PLCB1 cDNA ORF clone, Bos taurus(cattle)

Gene
Clones
gRNAs

Summary

Gene Symbol	PLCB1
Entrez Gene ID	287026
Full Name	phospholipase C beta 1
General protein information	Preferred Names phospholipase C beta 1 Names 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase beta-1 PLC-154 PLC-I PLC-beta-1 phosphoinositide phospholipase C phosphoinositide phospholipase C-beta-1 phospholipase C, beta 1 (phosphoinositide-specific)
Gene Type	protein-coding
Organism	Bos taurus(cattle)

mRNA and Protein(s)

mRNA	Protein	Name
NM_174817.1	NP_777242.1	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase beta-1
XM_005214055.3	XP_005214112.1	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase beta-1 isoform X1
XM_005214055.4	XP_005214112.1	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase beta-1 isoform X1

Pathways from BioSystems

Homologies

Macaca mulatta (Rhesus monkey)	PLCB1	XP_001116017.1
Mus musculus (house mouse)	Plcb1	NP_001139302.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP009115	XP_319865.4
Canis lupus familiaris (dog)	PLCB1	XP_005634858.1
Bos taurus (cattle)	PLCB1	NP_777242.1
Gallus gallus (chicken)	PLCB1	XP_425261.3
Drosophila melanogaster (fruit fly)	Plc21C	NP_995604.2
Homo sapiens (human)	PLCB1	NP_056007.1
Pan troglodytes (chimpanzee)	PLCB1	XP_001170216.3
Rattus norvegicus (Norway rat)	Plcb1	NP_001071109.1

Gene Ontology
Bibliography

Related articles in PubMed

A whole-genome assembly of the domestic cow, Bos taurus.
Zimin AV, Delcher AL, Florea L, Kelley DR, Schatz MC, Puiu D, Hanrahan F, Pertea G, Van Tassell CP, Sonstegard TS, Marçais G, Roberts M, Subramanian P, Yorke JA, Salzberg SL
Genome biology10(4)R42(2009)

Concentration of enzyme-dependent activation of PLC-beta 1 and PLC-beta 2 by G alpha 11 and beta gamma-subunits.
Paterson A, Boyer JL, Watts VJ, Morris AJ, Price EM, Harden TK
Cellular signalling7(7)709-20(1995 Sep)

Determination of the primary structure of PLC-154 demonstrates diversity of phosphoinositide-specific phospholipase C activities.
Katan M, Kriz RW, Totty N, Philp R, Meldrum E, Aldape RA, Knopf JL, Parker PJ
Cell54(2)171-7(1988 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following PLCB1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the PLCB1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OBa41627	NM_174817.1 Latest version!	Bos taurus phospholipase C beta 1 (PLCB1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OBa221455	XM_005214055.4 Latest version!	Bos taurus phospholipase C beta 1 (PLCB1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OBa24969	XM_005214055.3 Latest version!	Bos taurus phospholipase C beta 1 (PLCB1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

PLCB1 ( NM_174817.1 ) cDNA ORF clone, Bos taurus(cattle) -> NP_777242.1 Bos taurus phospholipase C beta 1 (PLCB1), mRNA.

Price & Availability↑

CloneID	OBa41627
Clone ID Related Accession (Same CDS sequence)	NM_174817.1
Accession Version	NM_174817.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3651bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1571328000000
Organism	Bos taurus(cattle)
Product	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase beta-1
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from J03137.1. On Mar 29, 2005 this sequence version replaced XM_598713.1. ##Evidence-Data-START## Transcript exon combination :: J03137.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN03145413, SAMN03145431 [ECO:0000348] ##Evidence-Data-END##

ATGGCCGGGG CACAGCCTGG AGTGCACGCG CTGCAACTCA AGCCGGTGTG CGTGTCCGAC 
AGCCTCAAGA AGGGCACCAA ATTCGTCAAG TGGGAGGATG ACTCCACTGT TGTCACTCCG 
ATTATTTTGA GGACTGACCC TCAGGGATTT TTCTTTTACT GGACAGATCA AAACAAGGAG 
ACGGAGCTGT TAGATCTCAG CCTTGTCAAA GACGCCAGAT GTGGGAAACA TGCCAAGGCG 
CCCAAGGACC CTAAATTACG TGAACTTCTG GATGTGGGGA ACATTGGGCG CCTGGAACAT 
CGCATGATCA CAGTGGTGTA TGGGCCTGAC TTAGTGAACA TCTCCCATTT GAACCTCGTG 
GCTTTCCAAG AAGAAGTGGC CAAGGAATGG ACAAATGAGG TTTTCAGTTT GGCAACAAAC 
CTGCTGGCCC AAAACATGTC CAGGGATGCA TTTCTGGAAA AGGCGTATAC TAAGCTTAAG 
CTGCAAGTCA CTCCAGAAGG ACGCATTCCT CTCAAAAACA TATACCGCCT GTTCTCAGCA 
GACCGGAAGC GAGTCGAGAC AGCTTTAGAG GCTTGTAGTC TTCCATCTTC AAGGAATGAT 
TCGATACCTC AAGAAGATTT CACTCCTGAA GTGTACAGAG TTTTCCTGAA CAACCTTTGT 
CCTCGACCTG AAATTGATAA CATCTTTTCC GAATTTGGTG CCAAAAGCAA ACCATACCTT 
ACAGTTGATC AGATGATGGA TTTTATCAAC CTCAAGCAGC GAGATCCCCG GCTAAATGAA 
ATACTTTACC CACCTTTAAA GCAAGAGCAA GTCCAAGTGT TGATTGAGAA GTATGAACCT 
AACAACAGCC TCGCCAAAAA AGGACAGATA TCAGTGGATG GATTTATGCG CTATCTGAGT 
GGAGAAGAAA ATGGAGTCGT TTCACCTGAG AAACTGGATT TGAATGAAGA TATGTCTCAG 
CCCCTTTCTC ACTATTTCAT TAATTCCTCA CACAACACCT ACCTCACAGC TGGCCAGCTG 
GCTGGAAACT CCTCCGTTGA GATGTATCGC CAAGTGCTCC TGTCTGGTTG TCGCTGTGTG 
GAGCTGGACT GCTGGAAGGG ACGAACTGCA GAAGAGGAGC CAGTCATCAC CCATGGGTTC 
ACCATGACCA CAGAAATATC CTTCAAGGAA GTGATTGAAG CCATTGCTGA ATGTGCATTT 
AAGACTTCAC CTTTTCCAAT TCTGCTTTCA TTTGAGAACC ATGTGGACTC GCCGAAGCAG 
CAAGCCAAGA TGGCGGAGTA CTGCAGACTC ATCTTCGGCG ACGCCCTCCT CATGGAGCCG 
CTGGACAAGT ACCCGTTGGA ATCTGGAGTT CCCCTTCCAA GTCCTATGGA TTTAATGTAC 
AAAATTTTGG TGAAAAATAA GAAGAAATCA CACAAGTCGT CAGAAGGAAG TGGCAAGAAG 
AAGCTCTCAG AACAAGCTTC CAACACGTAC AGCGACTCCT CCAGTGTGTT TGAGCCCTCG 
TCCCCAGGAG CCGGAGAAGC TGATACAGAG AGTGACGATG ATGACGATGA TGATGACTGT 
AAAAAGTCTT CAATGGATGA GGGGACCGCA GGGAGCGAGG CCATGGCCAC AGAAGAGATG 
TCTAATCTGG TGAACTATAT CCAGCCAGTC AAGTTCGAGT CATTTGAAAT TTCAAAAAAA 
AGAAATAGAA GCTTCGAGAT GTCTTCCTTT GTGGAAACCA AAGGTCTTGA GCAACTTACA 
AAGTCTCCGG TGGAATTTGT AGAATATAAC AAAATGCAGC TTAGCAGGAT ATATCCAAAG 
GGAACACGCG TGGATTCATC TAACTATATG CCTCAGCTCT TCTGGAATGC AGGCTGCCAA 
ATGGTTGCTC TTAATTTCCA AACAGTGGAC CTGGCTATGC AGATAAACAT GGGGATGTAT 
GAATATAATG GGAAGAGTGG CTACAGGCTG AAGCCGGAGT TCATGAGGAG ACCTGACAAG 
CACTTTGATC CATTCACAGA AGGCATCGTG GACGGCATAG TGGCCAACAC TTTATCTGTT 
AAGATTATTT CAGGTCAGTT TCTCTCTGAT AAGAAAGTCG GGACTTATGT GGAAGTGGAT 
ATGTTTGGTT TGCCTGTGGA CACAAGGAGG AAGGCATTTA AGACCAAAAC ATCACAAGGA 
AACGCTGTAA ATCCCATCTG GGAAGAAGAA CCCATTGTAT TCAAAAAGGT GGTCCTTCCT 
TCGCTAGCCT GTTTGAGGAT AGCAGTTTAT GAAGAAGGAG GTAAATTTAT TGGTCACCGA 
ATCTTGCCAG TACAAGCAAT TCGGCCAGGC TATCACTACA TCTGTCTGAG GAATGAGAGG 
AACCAGCCTC TCATGCTGCC AGCGCTCTTT GTCTACATAG AGGTGAAGGA CTATGTCCCA 
GACACATATG CAGATGTGAT TGAAGCTTTG TCAAATCCAA TCCGATATGT GAATCTGATG 
GAGCAGAGAG CTAAACAACT GGCTGCTTTG ACACTGGAAG ATGAAGAAGA AGTGAAGAAA 
GAGGCTGATC CTGGGGAAAC ACCATCAGAA GCTCCGAGTG AAGCCAGGCC AACTCCAGCA 
GAAAACGGGG TGAATCACAC AACGTCCCTG ACACCCAAAC CACCCTCCCA GGCTCTGCAC 
AGCCAGCCAG CTCCAGGTTC TGTAAAGGCA CCTGCCAAAA CAGAAGATCT TATTCAGAGT 
GTTTTAACAG AAGTAGAAGC GCAGACGATT GAAGAGCTAA AGCAGCAGAA GTCGTTTGTG 
AAACTTCAAA AGAAGCACTA CAAGGAAATG AAGGACCTGG TTAAGAGACA CCACAAGAAA 
ACCACCGACC TCATCAAAGA GCACACTACA AAATACAATG AAATCCAGAA TGACTACTTG 
AGAAGGAGAG CAGCTCTGGA AAAGACAGCC AAAAAGGACA ATAAGAAAAA ATCAGAACCC 
AGTAGCCCTG ACCACGTCTC ATCAACAATT GAGCAAGACC TTGCTGCCCT GGATGCCGAG 
ATGACCCAAA AGTTAGTAGA TCTGAAGGAC AAACAGCAGC AGCAGCTGCT TAATCTTCGA 
CAAGAGCAAT ACTATAGCGA AAAATACCAG AAACGAGAAC ACATTAAACT GCTTATTCAA 
AAATTGACTG ATGTTGCTGA AGAATGTCAG AATAATCAGT TAAAGAAGCT CAAAGAAATC 
TGTGAGAAAG AGAAGAAGGA ATTAAAGAAG AAAATGGATA AAAAGAGGCA GGAGAAGATA 
ACAGAAGCAA AATCCAAAGA CAAAAGCCAG ATGGAGGAGG AAAAGACAGA GATGATCCGG 
TCATACATCC AGGAGGTGGT GCAGTACATC AAGAGGTTAG AAGAGGCGCA AAGTAAACGG 
CAAGAAAAAC TCGTAGAGAA ACACAAAGAA ATCCGCCAGC AGATCCTGGA TGAAAAGCCC 
AAGCTACAGG TGGAACTGGA GCAGGAATAC CAAGACAAGT TCAAAAGGCT GCCCCTGGAG 
ATTTTGGAAT TTGTGCAGGA AGCCATGAAA GGGAAGATTA GTGAAGACAG CAATCACAGC 
TCTGCCCCTC CCCTGATGAC CTCAGACTCT GGAAAACTAA ACCAGAAGCC TCCTTCCAGT 
GAAGAATTGG AAGGAGAAAA CCCAGGAAAA GAGTTTGATA CCCCTCTGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_777242.1
CDS	13..3663
Translation	MAGAQPGVHALQLKPVCVSDSLKKGTKFVKWEDDSTVVTPIILRTDPQGFFFYWTDQNKETELLDLSLVKDARCGKHAKAPKDPKLRELLDVGNIGRLEHRMITVVYGPDLVNISHLNLVAFQEEVAKEWTNEVFSLATNLLAQNMSRDAFLEKAYTKLKLQVTPEGRIPLKNIYRLFSADRKRVETALEACSLPSSRNDSIPQEDFTPEVYRVFLNNLCPRPEIDNIFSEFGAKSKPYLTVDQMMDFINLKQRDPRLNEILYPPLKQEQVQVLIEKYEPNNSLAKKGQISVDGFMRYLSGEENGVVSPEKLDLNEDMSQPLSHYFINSSHNTYLTAGQLAGNSSVEMYRQVLLSGCRCVELDCWKGRTAEEEPVITHGFTMTTEISFKEVIEAIAECAFKTSPFPILLSFENHVDSPKQQAKMAEYCRLIFGDALLMEPLDKYPLESGVPLPSPMDLMYKILVKNKKKSHKSSEGSGKKKLSEQASNTYSDSSSVFEPSSPGAGEADTESDDDDDDDDCKKSSMDEGTAGSEAMATEEMSNLVNYIQPVKFESFEISKKRNRSFEMSSFVETKGLEQLTKSPVEFVEYNKMQLSRIYPKGTRVDSSNYMPQLFWNAGCQMVALNFQTVDLAMQINMGMYEYNGKSGYRLKPEFMRRPDKHFDPFTEGIVDGIVANTLSVKIISGQFLSDKKVGTYVEVDMFGLPVDTRRKAFKTKTSQGNAVNPIWEEEPIVFKKVVLPSLACLRIAVYEEGGKFIGHRILPVQAIRPGYHYICLRNERNQPLMLPALFVYIEVKDYVPDTYADVIEALSNPIRYVNLMEQRAKQLAALTLEDEEEVKKEADPGETPSEAPSEARPTPAENGVNHTTSLTPKPPSQALHSQPAPGSVKAPAKTEDLIQSVLTEVEAQTIEELKQQKSFVKLQKKHYKEMKDLVKRHHKKTTDLIKEHTTKYNEIQNDYLRRRAALEKTAKKDNKKKSEPSSPDHVSSTIEQDLAALDAEMTQKLVDLKDKQQQQLLNLRQEQYYSEKYQKREHIKLLIQKLTDVAEECQNNQLKKLKEICEKEKKELKKKMDKKRQEKITEAKSKDKSQMEEEKTEMIRSYIQEVVQYIKRLEEAQSKRQEKLVEKHKEIRQQILDEKPKLQVELEQEYQDKFKRLPLEILEFVQEAMKGKISEDSNHSSAPPLMTSDSGKLNQKPPSSEELEGENPGKEFDTPL

Target ORF information:

RefSeq Version	NM_174817.1
Organism	Bos taurus(cattle)
Definition	Bos taurus phospholipase C beta 1 (PLCB1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_174817.1

ORF Insert Sequence:

ATGGCCGGGG CACAGCCTGG AGTGCACGCG CTGCAACTCA AGCCGGTGTG CGTGTCCGAC 
AGCCTCAAGA AGGGCACCAA ATTCGTCAAG TGGGAGGATG ACTCCACTGT TGTCACTCCG 
ATTATTTTGA GGACTGACCC TCAGGGATTT TTCTTTTACT GGACAGATCA AAACAAGGAG 
ACGGAGCTGT TAGATCTCAG CCTTGTCAAA GACGCCAGAT GTGGGAAACA TGCCAAGGCG 
CCCAAGGACC CTAAATTACG TGAACTTCTG GATGTGGGGA ACATTGGGCG CCTGGAACAT 
CGCATGATCA CAGTGGTGTA TGGGCCTGAC TTAGTGAACA TCTCCCATTT GAACCTCGTG 
GCTTTCCAAG AAGAAGTGGC CAAGGAATGG ACAAATGAGG TTTTCAGTTT GGCAACAAAC 
CTGCTGGCCC AAAACATGTC CAGGGATGCA TTTCTGGAAA AGGCGTATAC TAAGCTTAAG 
CTGCAAGTCA CTCCAGAAGG ACGCATTCCT CTCAAAAACA TATACCGCCT GTTCTCAGCA 
GACCGGAAGC GAGTCGAGAC AGCTTTAGAG GCTTGTAGTC TTCCATCTTC AAGGAATGAT 
TCGATACCTC AAGAAGATTT CACTCCTGAA GTGTACAGAG TTTTCCTGAA CAACCTTTGT 
CCTCGACCTG AAATTGATAA CATCTTTTCC GAATTTGGTG CCAAAAGCAA ACCATACCTT 
ACAGTTGATC AGATGATGGA TTTTATCAAC CTCAAGCAGC GAGATCCCCG GCTAAATGAA 
ATACTTTACC CACCTTTAAA GCAAGAGCAA GTCCAAGTGT TGATTGAGAA GTATGAACCT 
AACAACAGCC TCGCCAAAAA AGGACAGATA TCAGTGGATG GATTTATGCG CTATCTGAGT 
GGAGAAGAAA ATGGAGTCGT TTCACCTGAG AAACTGGATT TGAATGAAGA TATGTCTCAG 
CCCCTTTCTC ACTATTTCAT TAATTCCTCA CACAACACCT ACCTCACAGC TGGCCAGCTG 
GCTGGAAACT CCTCCGTTGA GATGTATCGC CAAGTGCTCC TGTCTGGTTG TCGCTGTGTG 
GAGCTGGACT GCTGGAAGGG ACGAACTGCA GAAGAGGAGC CAGTCATCAC CCATGGGTTC 
ACCATGACCA CAGAAATATC CTTCAAGGAA GTGATTGAAG CCATTGCTGA ATGTGCATTT 
AAGACTTCAC CTTTTCCAAT TCTGCTTTCA TTTGAGAACC ATGTGGACTC GCCGAAGCAG 
CAAGCCAAGA TGGCGGAGTA CTGCAGACTC ATCTTCGGCG ACGCCCTCCT CATGGAGCCG 
CTGGACAAGT ACCCGTTGGA ATCTGGAGTT CCCCTTCCAA GTCCTATGGA TTTAATGTAC 
AAAATTTTGG TGAAAAATAA GAAGAAATCA CACAAGTCGT CAGAAGGAAG TGGCAAGAAG 
AAGCTCTCAG AACAAGCTTC CAACACGTAC AGCGACTCCT CCAGTGTGTT TGAGCCCTCG 
TCCCCAGGAG CCGGAGAAGC TGATACAGAG AGTGACGATG ATGACGATGA TGATGACTGT 
AAAAAGTCTT CAATGGATGA GGGGACCGCA GGGAGCGAGG CCATGGCCAC AGAAGAGATG 
TCTAATCTGG TGAACTATAT CCAGCCAGTC AAGTTCGAGT CATTTGAAAT TTCAAAAAAA 
AGAAATAGAA GCTTCGAGAT GTCTTCCTTT GTGGAAACCA AAGGTCTTGA GCAACTTACA 
AAGTCTCCGG TGGAATTTGT AGAATATAAC AAAATGCAGC TTAGCAGGAT ATATCCAAAG 
GGAACACGCG TGGATTCATC TAACTATATG CCTCAGCTCT TCTGGAATGC AGGCTGCCAA 
ATGGTTGCTC TTAATTTCCA AACAGTGGAC CTGGCTATGC AGATAAACAT GGGGATGTAT 
GAATATAATG GGAAGAGTGG CTACAGGCTG AAGCCGGAGT TCATGAGGAG ACCTGACAAG 
CACTTTGATC CATTCACAGA AGGCATCGTG GACGGCATAG TGGCCAACAC TTTATCTGTT 
AAGATTATTT CAGGTCAGTT TCTCTCTGAT AAGAAAGTCG GGACTTATGT GGAAGTGGAT 
ATGTTTGGTT TGCCTGTGGA CACAAGGAGG AAGGCATTTA AGACCAAAAC ATCACAAGGA 
AACGCTGTAA ATCCCATCTG GGAAGAAGAA CCCATTGTAT TCAAAAAGGT GGTCCTTCCT 
TCGCTAGCCT GTTTGAGGAT AGCAGTTTAT GAAGAAGGAG GTAAATTTAT TGGTCACCGA 
ATCTTGCCAG TACAAGCAAT TCGGCCAGGC TATCACTACA TCTGTCTGAG GAATGAGAGG 
AACCAGCCTC TCATGCTGCC AGCGCTCTTT GTCTACATAG AGGTGAAGGA CTATGTCCCA 
GACACATATG CAGATGTGAT TGAAGCTTTG TCAAATCCAA TCCGATATGT GAATCTGATG 
GAGCAGAGAG CTAAACAACT GGCTGCTTTG ACACTGGAAG ATGAAGAAGA AGTGAAGAAA 
GAGGCTGATC CTGGGGAAAC ACCATCAGAA GCTCCGAGTG AAGCCAGGCC AACTCCAGCA 
GAAAACGGGG TGAATCACAC AACGTCCCTG ACACCCAAAC CACCCTCCCA GGCTCTGCAC 
AGCCAGCCAG CTCCAGGTTC TGTAAAGGCA CCTGCCAAAA CAGAAGATCT TATTCAGAGT 
GTTTTAACAG AAGTAGAAGC GCAGACGATT GAAGAGCTAA AGCAGCAGAA GTCGTTTGTG 
AAACTTCAAA AGAAGCACTA CAAGGAAATG AAGGACCTGG TTAAGAGACA CCACAAGAAA 
ACCACCGACC TCATCAAAGA GCACACTACA AAATACAATG AAATCCAGAA TGACTACTTG 
AGAAGGAGAG CAGCTCTGGA AAAGACAGCC AAAAAGGACA ATAAGAAAAA ATCAGAACCC 
AGTAGCCCTG ACCACGTCTC ATCAACAATT GAGCAAGACC TTGCTGCCCT GGATGCCGAG 
ATGACCCAAA AGTTAGTAGA TCTGAAGGAC AAACAGCAGC AGCAGCTGCT TAATCTTCGA 
CAAGAGCAAT ACTATAGCGA AAAATACCAG AAACGAGAAC ACATTAAACT GCTTATTCAA 
AAATTGACTG ATGTTGCTGA AGAATGTCAG AATAATCAGT TAAAGAAGCT CAAAGAAATC 
TGTGAGAAAG AGAAGAAGGA ATTAAAGAAG AAAATGGATA AAAAGAGGCA GGAGAAGATA 
ACAGAAGCAA AATCCAAAGA CAAAAGCCAG ATGGAGGAGG AAAAGACAGA GATGATCCGG 
TCATACATCC AGGAGGTGGT GCAGTACATC AAGAGGTTAG AAGAGGCGCA AAGTAAACGG 
CAAGAAAAAC TCGTAGAGAA ACACAAAGAA ATCCGCCAGC AGATCCTGGA TGAAAAGCCC 
AAGCTACAGG TGGAACTGGA GCAGGAATAC CAAGACAAGT TCAAAAGGCT GCCCCTGGAG 
ATTTTGGAAT TTGTGCAGGA AGCCATGAAA GGGAAGATTA GTGAAGACAG CAATCACAGC 
TCTGCCCCTC CCCTGATGAC CTCAGACTCT GGAAAACTAA ACCAGAAGCC TCCTTCCAGT 
GAAGAATTGG AAGGAGAAAA CCCAGGAAAA GAGTTTGATA CCCCTCTGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PLCB1 ( XM_005214055.4 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005214112.1 Bos taurus phospholipase C beta 1 (PLCB1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OBa221455
Clone ID Related Accession (Same CDS sequence)	XM_005214055.4
Accession Version	XM_005214055.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3519bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1525536000000
Organism	Bos taurus(cattle)
Product	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase beta-1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037340.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005214055.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCGGGG CACAGCCTGG AGTGCACGCG CTGCAACTCA AGCCGGTGTG CGTGTCCGAC 
AGCCTCAAGA AGGGCACCAA ATTCGTCAAG TGGGAGGATG ACTCCACTGT TGTCACTCCG 
ATTATTTTGA GGACTGACCC TCAGGGATTT TTCTTTTACT GGACAGATCA AAACAAGGAG 
ACGGAGCTGT TAGATCTCAG CCTTGTCAAA GACGCCAGAT GTGGGAAACA TGCCAAGGCG 
CCCAAGGACC CTAAATTACG TGAACTTCTG GATGTGGGGA ACATTGGGCG CCTGGAACAT 
CGCATGATCA CAGTGGTGTA TGGGCCTGAC TTAGTGAACA TCTCCCATTT GAACCTCGTG 
GCTTTCCAAG AAGAAGTGGC CAAGGAATGG ACAAATGAGG TTTTCAGTTT GGCAACAAAC 
CTGCTGGCCC AAAACATGTC CAGGGATGCA TTTCTGGAAA AGGCGTATAC TAAGCTTAAG 
CTGCAAGTCA CTCCAGAAGG ACGCATTCCT CTCAAAAACA TATACCGCCT GTTCTCAGCA 
GACCGGAAGC GAGTCGAGAC AGCTTTAGAG GCTTGTAGTC TTCCATCTTC AAGGAATGAT 
TCGATACCTC AAGAAGATTT CACTCCTGAA GTGTACAGAG TTTTCCTGAA CAACCTTTGT 
CCTCGACCTG AAATTGATAA CATCTTTTCC GAATTTGGTG CCAAAAGCAA ACCATACCTT 
ACAGTTGATC AGATGATGGA TTTTATCAAC CTCAAGCAGC GAGATCCCCG GCTAAATGAA 
ATACTTTACC CACCTTTAAA GCAAGAGCAA GTCCAAGTGT TGATTGAGAA GTATGAACCT 
AACAACAGCC TCGCCAAAAA AGGACAGATA TCAGTGGATG GATTTATGCG CTATCTGAGT 
GGAGAAGAAA ATGGAGTCGT TTCACCTGAG AAACTGGATT TGAATGAAGA TATGTCTCAG 
CCCCTTTCTC ACTATTTCAT TAATTCCTCA CACAACACCT ACCTCACAGC TGGCCAGCTG 
GCTGGAAACT CCTCCGTTGA GATGTATCGC CAAGTGCTCC TGTCTGGTTG TCGCTGTGTG 
GAGCTGGACT GCTGGAAGGG ACGAACTGCA GAAGAGGAGC CAGTCATCAC CCATGGGTTC 
ACCATGACCA CAGAAATATC CTTCAAGGAA GTGATTGAAG CCATTGCTGA ATGTGCATTT 
AAGACTTCAC CTTTTCCAAT TCTGCTTTCA TTTGAGAACC ATGTGGACTC GCCGAAGCAG 
CAAGCCAAGA TGGCGGAGTA CTGCAGACTC ATCTTCGGCG ACGCCCTCCT CATGGAGCCG 
CTGGACAAGT ACCCGTTGGA ATCTGGAGTT CCCCTTCCAA GTCCTATGGA TTTAATGTAC 
AAAATTTTGG TGAAAAATAA GAAGAAATCA CACAAGTCGT CAGAAGGAAG TGGCAAGAAG 
AAGCTCTCAG AACAAGCTTC CAACACGTAC AGCGACTCCT CCAGTGTGTT TGAGCCCTCG 
TCCCCAGGAG CCGGAGAAGC TGATACAGAG AGTGACGATG ATGACGATGA TGATGACTGT 
AAAAAGTCTT CAATGGATGA GGGGACCGCA GGGAGCGAGG CCATGGCCAC AGAAGAGATG 
TCTAATCTGG TGAACTATAT CCAGCCAGTC AAGTTCGAGT CATTTGAAAT TTCAAAAAAA 
AGAAATAGAA GCTTCGAGAT GTCTTCCTTT GTGGAAACCA AAGGTCTTGA GCAACTTACA 
AAGTCTCCGG TGGAATTTGT AGAATATAAC AAAATGCAGC TTAGCAGGAT ATATCCAAAG 
GGAACACGCG TGGATTCATC TAACTATATG CCTCAGCTCT TCTGGAATGC AGGCTGCCAA 
ATGGTTGCTC TTAATTTCCA AACAGTGGAC CTGGCTATGC AGATAAACAT GGGGATGTAT 
GAATATAATG GGAAGAGTGG CTACAGGCTG AAGCCGGAGT TCATGAGGAG ACCTGACAAG 
CACTTTGATC CATTCACAGA AGGCATCGTG GACGGCATAG TGGCCAACAC TTTATCTGTT 
AAGATTATTT CAGGTCAGTT TCTCTCTGAT AAGAAAGTCG GGACTTATGT GGAAGTGGAT 
ATGTTTGGTT TGCCTGTGGA CACAAGGAGG AAGGCATTTA AGACCAAAAC ATCACAAGGA 
AACGCTGTAA ATCCCATCTG GGAAGAAGAA CCCATTGTAT TCAAAAAGGT GGTTCTTCCT 
TCGCTAGCCT GTCTGAGGAT AGCAGTTTAT GAAGAAGGAG GTAAATTTAT TGGTCACCGA 
ATCTTGCCAG TACAAGCAAT TCGGCCAGGC TATCACTACA TCTGTCTGAG GAATGAGAGG 
AACCAGCCTC TCATGCTGCC AGCGCTCTTT GTCTACATAG AGGTGAAGGA CTATGTCCCA 
GACACATATG CAGATGTGAT TGAAGCTTTG TCAAATCCAA TCCGATATGT GAATCTGATG 
GAGCAGAGAG CTAAACAACT GGCTGCTTTG ACACTGGAAG ATGAAGAAGA AGTGAAGAAA 
GAGGCTGATC CTGGGGAAAC ACCATCAGAA GCTCCGAGTG AAGCCAGGCC AACTCCAGCA 
GAAAACGGGG TGAATCACAC AACGTCCCTG ACACCCAAAC CACCCTCCCA GGCTCTGCAC 
AGCCAGCCAG CTCCAGGTTC TGTAAAGGCA CCTGCCAAAA CAGAAGATCT TATTCAGAGT 
GTTTTAACAG AAGTAGAAGC GCAGACGATT GAAGAGCTAA AGCAGCAGAA GTCGTTTGTG 
AAACTTCAAA AGAAGCACTA CAAGGAAATG AAGGACCTGG TTAAGAGACA CCACAAGAAA 
ACCACCGACC TCATCAAAGA GCACACTACA AAATACAATG AAATCCAGAA CGACTACTTG 
AGAAGGAGAG CAGCTCTGGA AAAGACAGCC AAAAAGGACA ATAAGAAAAA ATCAGAACCC 
AGTAGCCCTG ACCACGTCTC ATCAACAATT GAGCAAGACC TTGCTGCCCT GGATGCCGAG 
ATGACCCAAA AGTTAGTAGA TCTGAAGGAC AAACAGCAGC AGCAGCTGCT TAATCTTCGA 
CAAGAGCAAT ACTATAGCGA AAAATACCAG AAACGAGAAC ACATTAAACT GCTTATTCAA 
AAATTGACTG ATGTTGCTGA AGAATGTCAG AATAATCAGT TAAAGAAGCT CAAAGAAATC 
TGTGAGAAAG AGAAGAAGGA ATTAAAGAAG AAAATGGATA AAAAGAGGCA GGAGAAGATA 
ACAGAAGCAA AATCCAAAGA CAAAAGCCAG ATGGAGGAGG AAAAGACAGA GATGATCCGG 
TCATACATCC AGGAGGTGGT GCAGTACATC AAGAGGTTAG AAGAGGCGCA AAGTAAACGG 
CAAGAAAAAC TCGTAGAGAA ACACAAAGAA ATCCGCCAGC AGATCCTGGA TGAAAAGCCC 
AAGGGGGACG GTTCCTCCTC ATTCTTGTCG GAAACTTGCC ATGAGGATCC CTCTGTTTCC 
CCCAACTTTA CTCCCCCCAA CCCTCAAGCT CTCAAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005214112.1
CDS	70..3588
Translation	MAGAQPGVHALQLKPVCVSDSLKKGTKFVKWEDDSTVVTPIILRTDPQGFFFYWTDQNKETELLDLSLVKDARCGKHAKAPKDPKLRELLDVGNIGRLEHRMITVVYGPDLVNISHLNLVAFQEEVAKEWTNEVFSLATNLLAQNMSRDAFLEKAYTKLKLQVTPEGRIPLKNIYRLFSADRKRVETALEACSLPSSRNDSIPQEDFTPEVYRVFLNNLCPRPEIDNIFSEFGAKSKPYLTVDQMMDFINLKQRDPRLNEILYPPLKQEQVQVLIEKYEPNNSLAKKGQISVDGFMRYLSGEENGVVSPEKLDLNEDMSQPLSHYFINSSHNTYLTAGQLAGNSSVEMYRQVLLSGCRCVELDCWKGRTAEEEPVITHGFTMTTEISFKEVIEAIAECAFKTSPFPILLSFENHVDSPKQQAKMAEYCRLIFGDALLMEPLDKYPLESGVPLPSPMDLMYKILVKNKKKSHKSSEGSGKKKLSEQASNTYSDSSSVFEPSSPGAGEADTESDDDDDDDDCKKSSMDEGTAGSEAMATEEMSNLVNYIQPVKFESFEISKKRNRSFEMSSFVETKGLEQLTKSPVEFVEYNKMQLSRIYPKGTRVDSSNYMPQLFWNAGCQMVALNFQTVDLAMQINMGMYEYNGKSGYRLKPEFMRRPDKHFDPFTEGIVDGIVANTLSVKIISGQFLSDKKVGTYVEVDMFGLPVDTRRKAFKTKTSQGNAVNPIWEEEPIVFKKVVLPSLACLRIAVYEEGGKFIGHRILPVQAIRPGYHYICLRNERNQPLMLPALFVYIEVKDYVPDTYADVIEALSNPIRYVNLMEQRAKQLAALTLEDEEEVKKEADPGETPSEAPSEARPTPAENGVNHTTSLTPKPPSQALHSQPAPGSVKAPAKTEDLIQSVLTEVEAQTIEELKQQKSFVKLQKKHYKEMKDLVKRHHKKTTDLIKEHTTKYNEIQNDYLRRRAALEKTAKKDNKKKSEPSSPDHVSSTIEQDLAALDAEMTQKLVDLKDKQQQQLLNLRQEQYYSEKYQKREHIKLLIQKLTDVAEECQNNQLKKLKEICEKEKKELKKKMDKKRQEKITEAKSKDKSQMEEEKTEMIRSYIQEVVQYIKRLEEAQSKRQEKLVEKHKEIRQQILDEKPKGDGSSSFLSETCHEDPSVSPNFTPPNPQALK

Target ORF information:

RefSeq Version	XM_005214055.4
Organism	Bos taurus(cattle)
Definition	Bos taurus phospholipase C beta 1 (PLCB1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005214055.4

ORF Insert Sequence:

ATGGCCGGGG CACAGCCTGG AGTGCACGCG CTGCAACTCA AGCCGGTGTG CGTGTCCGAC 
AGCCTCAAGA AGGGCACCAA ATTCGTCAAG TGGGAGGATG ACTCCACTGT TGTCACTCCG 
ATTATTTTGA GGACTGACCC TCAGGGATTT TTCTTTTACT GGACAGATCA AAACAAGGAG 
ACGGAGCTGT TAGATCTCAG CCTTGTCAAA GACGCCAGAT GTGGGAAACA TGCCAAGGCG 
CCCAAGGACC CTAAATTACG TGAACTTCTG GATGTGGGGA ACATTGGGCG CCTGGAACAT 
CGCATGATCA CAGTGGTGTA TGGGCCTGAC TTAGTGAACA TCTCCCATTT GAACCTCGTG 
GCTTTCCAAG AAGAAGTGGC CAAGGAATGG ACAAATGAGG TTTTCAGTTT GGCAACAAAC 
CTGCTGGCCC AAAACATGTC CAGGGATGCA TTTCTGGAAA AGGCGTATAC TAAGCTTAAG 
CTGCAAGTCA CTCCAGAAGG ACGCATTCCT CTCAAAAACA TATACCGCCT GTTCTCAGCA 
GACCGGAAGC GAGTCGAGAC AGCTTTAGAG GCTTGTAGTC TTCCATCTTC AAGGAATGAT 
TCGATACCTC AAGAAGATTT CACTCCTGAA GTGTACAGAG TTTTCCTGAA CAACCTTTGT 
CCTCGACCTG AAATTGATAA CATCTTTTCC GAATTTGGTG CCAAAAGCAA ACCATACCTT 
ACAGTTGATC AGATGATGGA TTTTATCAAC CTCAAGCAGC GAGATCCCCG GCTAAATGAA 
ATACTTTACC CACCTTTAAA GCAAGAGCAA GTCCAAGTGT TGATTGAGAA GTATGAACCT 
AACAACAGCC TCGCCAAAAA AGGACAGATA TCAGTGGATG GATTTATGCG CTATCTGAGT 
GGAGAAGAAA ATGGAGTCGT TTCACCTGAG AAACTGGATT TGAATGAAGA TATGTCTCAG 
CCCCTTTCTC ACTATTTCAT TAATTCCTCA CACAACACCT ACCTCACAGC TGGCCAGCTG 
GCTGGAAACT CCTCCGTTGA GATGTATCGC CAAGTGCTCC TGTCTGGTTG TCGCTGTGTG 
GAGCTGGACT GCTGGAAGGG ACGAACTGCA GAAGAGGAGC CAGTCATCAC CCATGGGTTC 
ACCATGACCA CAGAAATATC CTTCAAGGAA GTGATTGAAG CCATTGCTGA ATGTGCATTT 
AAGACTTCAC CTTTTCCAAT TCTGCTTTCA TTTGAGAACC ATGTGGACTC GCCGAAGCAG 
CAAGCCAAGA TGGCGGAGTA CTGCAGACTC ATCTTCGGCG ACGCCCTCCT CATGGAGCCG 
CTGGACAAGT ACCCGTTGGA ATCTGGAGTT CCCCTTCCAA GTCCTATGGA TTTAATGTAC 
AAAATTTTGG TGAAAAATAA GAAGAAATCA CACAAGTCGT CAGAAGGAAG TGGCAAGAAG 
AAGCTCTCAG AACAAGCTTC CAACACGTAC AGCGACTCCT CCAGTGTGTT TGAGCCCTCG 
TCCCCAGGAG CCGGAGAAGC TGATACAGAG AGTGACGATG ATGACGATGA TGATGACTGT 
AAAAAGTCTT CAATGGATGA GGGGACCGCA GGGAGCGAGG CCATGGCCAC AGAAGAGATG 
TCTAATCTGG TGAACTATAT CCAGCCAGTC AAGTTCGAGT CATTTGAAAT TTCAAAAAAA 
AGAAATAGAA GCTTCGAGAT GTCTTCCTTT GTGGAAACCA AAGGTCTTGA GCAACTTACA 
AAGTCTCCGG TGGAATTTGT AGAATATAAC AAAATGCAGC TTAGCAGGAT ATATCCAAAG 
GGAACACGCG TGGATTCATC TAACTATATG CCTCAGCTCT TCTGGAATGC AGGCTGCCAA 
ATGGTTGCTC TTAATTTCCA AACAGTGGAC CTGGCTATGC AGATAAACAT GGGGATGTAT 
GAATATAATG GGAAGAGTGG CTACAGGCTG AAGCCGGAGT TCATGAGGAG ACCTGACAAG 
CACTTTGATC CATTCACAGA AGGCATCGTG GACGGCATAG TGGCCAACAC TTTATCTGTT 
AAGATTATTT CAGGTCAGTT TCTCTCTGAT AAGAAAGTCG GGACTTATGT GGAAGTGGAT 
ATGTTTGGTT TGCCTGTGGA CACAAGGAGG AAGGCATTTA AGACCAAAAC ATCACAAGGA 
AACGCTGTAA ATCCCATCTG GGAAGAAGAA CCCATTGTAT TCAAAAAGGT GGTTCTTCCT 
TCGCTAGCCT GTCTGAGGAT AGCAGTTTAT GAAGAAGGAG GTAAATTTAT TGGTCACCGA 
ATCTTGCCAG TACAAGCAAT TCGGCCAGGC TATCACTACA TCTGTCTGAG GAATGAGAGG 
AACCAGCCTC TCATGCTGCC AGCGCTCTTT GTCTACATAG AGGTGAAGGA CTATGTCCCA 
GACACATATG CAGATGTGAT TGAAGCTTTG TCAAATCCAA TCCGATATGT GAATCTGATG 
GAGCAGAGAG CTAAACAACT GGCTGCTTTG ACACTGGAAG ATGAAGAAGA AGTGAAGAAA 
GAGGCTGATC CTGGGGAAAC ACCATCAGAA GCTCCGAGTG AAGCCAGGCC AACTCCAGCA 
GAAAACGGGG TGAATCACAC AACGTCCCTG ACACCCAAAC CACCCTCCCA GGCTCTGCAC 
AGCCAGCCAG CTCCAGGTTC TGTAAAGGCA CCTGCCAAAA CAGAAGATCT TATTCAGAGT 
GTTTTAACAG AAGTAGAAGC GCAGACGATT GAAGAGCTAA AGCAGCAGAA GTCGTTTGTG 
AAACTTCAAA AGAAGCACTA CAAGGAAATG AAGGACCTGG TTAAGAGACA CCACAAGAAA 
ACCACCGACC TCATCAAAGA GCACACTACA AAATACAATG AAATCCAGAA CGACTACTTG 
AGAAGGAGAG CAGCTCTGGA AAAGACAGCC AAAAAGGACA ATAAGAAAAA ATCAGAACCC 
AGTAGCCCTG ACCACGTCTC ATCAACAATT GAGCAAGACC TTGCTGCCCT GGATGCCGAG 
ATGACCCAAA AGTTAGTAGA TCTGAAGGAC AAACAGCAGC AGCAGCTGCT TAATCTTCGA 
CAAGAGCAAT ACTATAGCGA AAAATACCAG AAACGAGAAC ACATTAAACT GCTTATTCAA 
AAATTGACTG ATGTTGCTGA AGAATGTCAG AATAATCAGT TAAAGAAGCT CAAAGAAATC 
TGTGAGAAAG AGAAGAAGGA ATTAAAGAAG AAAATGGATA AAAAGAGGCA GGAGAAGATA 
ACAGAAGCAA AATCCAAAGA CAAAAGCCAG ATGGAGGAGG AAAAGACAGA GATGATCCGG 
TCATACATCC AGGAGGTGGT GCAGTACATC AAGAGGTTAG AAGAGGCGCA AAGTAAACGG 
CAAGAAAAAC TCGTAGAGAA ACACAAAGAA ATCCGCCAGC AGATCCTGGA TGAAAAGCCC 
AAGGGGGACG GTTCCTCCTC ATTCTTGTCG GAAACTTGCC ATGAGGATCC CTCTGTTTCC 
CCCAACTTTA CTCCCCCCAA CCCTCAAGCT CTCAAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PLCB1 ( XM_005214055.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005214112.1 Bos taurus phospholipase C beta 1 (PLCB1), transcript variant X1, mRNA.

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CloneID	OBa24969
Clone ID Related Accession (Same CDS sequence)	XM_005214055.3
Accession Version	XM_005214055.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3519bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1453651200000
Organism	Bos taurus(cattle)
Product	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase beta-1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104359.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005214055.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCGGGG CACAGCCTGG AGTGCACGCG TTGCAACTCA AGCCGGTGTG CGTGTCCGAC 
AGCCTCAAGA AGGGCACCAA ATTCGTCAAG TGGGAGGATG ACTCCACTGT TGTCACTCCG 
ATTATTTTGA GGACTGACCC TCAGGGATTT TTCTTTTACT GGACAGATCA AAACAAGGAG 
ACGGAGCTGT TAGATCTCAG CCTTGTCAAA GACGCCAGAT GTGGGAAGCA TGCCAAGGCG 
CCCAAGGACC CTAAATTACG TGAACTTCTG GATGTGGGGA ACATTGGGCG CCTGGAACAT 
CGCATGATCA CAGTGGTGTA TGGGCCTGAC TTAGTGAACA TCTCCCATTT GAACCTCGTG 
GCTTTCCAAG AAGAAGTGGC CAAGGAATGG ACAAATGAGG TTTTCAGTTT GGCAACAAAC 
CTGCTGGCCC AAAACATGTC CAGGGATGCA TTTCTGGAAA AGGCGTATAC TAAGCTTAAG 
CTGCAAGTCA CTCCAGAAGG ACGCATTCCT CTCAAAAACA TATACCGCCT GTTCTCAGCA 
GACCGGAAGC GAGTCGAGAC AGCTTTAGAG GCTTGTAGTC TTCCATCTTC AAGGAATGAT 
TCGATACCTC AAGAAGATTT CACTCCTGAA GTGTACAGAG TTTTCCTGAA CAACCTTTGT 
CCTCGACCTG AAATTGATAA CATCTTTTCC GAATTTGGTG CCAAAAGCAA ACCATACCTT 
ACAGTTGATC AGATGATGGA TTTTATCAAC CTCAAGCAGC GAGATCCCCG GCTAAATGAA 
ATACTTTACC CACCTTTAAA GCAAGAGCAA GTCCAAGTGT TGATTGAGAA GTATGAACCT 
AACAACAGCC TCGCCAAAAA AGGACAGATA TCAGTGGATG GATTTATGCG CTATCTGAGT 
GGAGAAGAAA ATGGAGTCGT TTCACCTGAG AAACTGGATT TGAATGAAGA TATGTCTCAG 
CCCCTTTCTC ACTATTTCAT TAATTCCTCA CACAACACCT ACCTCACAGC TGGCCAGCTG 
GCTGGAAACT CCTCCGTTGA GATGTATCGC CAAGTGCTCC TGTCTGGTTG TCGCTGTGTG 
GAGCTGGACT GCTGGAAGGG ACGAACTGCA GAAGAGGAGC CAGTCATCAC CCATGGGTTC 
ACCATGACCA CAGAAATATC CTTCAAGGAA GTGATTGAAG CCATTGCTGA ATGTGCATTT 
AAGACTTCAC CTTTTCCAAT TCTGCTTTCA TTTGAGAACC ATGTGGACTC GCCGAAGCAG 
CAAGCCAAGA TGGCGGAGTA CTGCAGACTC ATCTTCGGCG ACGCCCTCCT CATGGAGCCG 
CTGGACAAGT ACCCGTTGGA ATCTGGAGTT CCCCTTCCAA GTCCTATGGA TTTAATGTAC 
AAAATTTTGG TGAAAAATAA GAAGAAATCA CACAAGTCGT CAGAAGGAAG TGGCAAGAAG 
AAGCTCTCAG AACAAGCTTC CAACACGTAC AGCGACTCCT CCAGTGTGTT TGAGCCCTCG 
TCCCCAGGAG CTGGAGAAGC TGATACAGAG AGTGACGATG ATGACGATGA TGATGACTGT 
AAAAAGTCTT CAATGGATGA GGGGACCGCA GGGAGCGAGG CCATGGCCAC AGAAGAGATG 
TCTAATCTGG TGAACTATAT CCAGCCAGTC AAGTTCGAGT CATTTGAAAT TTCAAAAAAA 
AGAAATAGAA GCTTCGAGAT GTCTTCCTTT GTGGAAACCA AAGGTCTTGA GCAACTTACA 
AAGTCTCCGG TGGAATTTGT AGAATATAAC AAAATGCAGC TTAGCAGGAT ATATCCAAAG 
GGAACACGCG TGGATTCATC TAACTATATG CCTCAGCTCT TCTGGAATGC AGGCTGCCAA 
ATGGTTGCTC TTAATTTCCA AACAGTGGAC CTGGCTATGC AGATAAACAT GGGGATGTAT 
GAATATAATG GGAAGAGTGG CTACAGGCTG AAGCCGGAGT TCATGAGGAG ACCTGACAAG 
CACTTTGATC CATTCACAGA AGGCATCGTG GACGGCATAG TGGCCAACAC TTTATCTGTT 
AAGATTATTT CAGGTCAGTT TCTCTCTGAT AAGAAAGTCG GGACTTATGT GGAAGTGGAT 
ATGTTTGGTT TGCCTGTGGA CACAAGGAGG AAGGCATTTA AGACCAAAAC ATCACAAGGA 
AACGCTGTAA ATCCCATCTG GGAAGAAGAA CCCATTGTAT TCAAAAAGGT GGTTCTTCCT 
TCGCTAGCCT GTCTGAGGAT AGCAGTTTAT GAAGAAGGAG GTAAATTTAT TGGTCACCGA 
ATCTTGCCAG TACAAGCAAT TCGGCCAGGC TATCACTACA TCTGTCTGAG GAATGAGAGG 
AACCAGCCTC TCATGCTGCC AGCGCTCTTT GTCTACATAG AGGTGAAGGA CTATGTCCCA 
GACACATATG CAGATGTGAT TGAAGCTTTG TCAAATCCAA TCCGATATGT GAATCTGATG 
GAGCAGAGAG CTAAACAACT GGCTGCTTTG ACACTGGAAG ATGAAGAAGA AGTGAAGAAA 
GAGGCTGATC CTGGGGAAAC ACCATCAGAA GCTCCGAGTG AAGCCAGGCC AACTCCAGCA 
GAAAACGGGG TGAATCACAC AACGTCCCTG ACACCCAAAC CACCCTCCCA GGCTCTGCAC 
AGCCAGCCAG CTCCAGGTTC TGTAAAGGCA CCTGCCAAAA CAGAAGATCT TATTCAGAGT 
GTTTTAACAG AAGTAGAAGC GCAGACGATT GAAGAGCTAA AGCAGCAGAA GTCGTTTGTG 
AAACTTCAAA AGAAGCACTA CAAGGAAATG AAGGACCTGG TTAAGAGACA CCACAAGAAA 
ACCACCGACC TCATCAAAGA GCACACTACA AAATACAATG AAATCCAGAA TGACTACTTG 
AGAAGGAGAG CAGCTCTGGA AAAGACAGCC AAAAAGGACA ATAAGAAAAA ATCAGAACCC 
AGTAGCCCTG ACCACGTCTC ATCAACAATT GAGCAAGACC TTGCTGCCCT GGATGCCGAG 
ATGACCCAAA AGTTAGTAGA TCTGAAGGAC AAACAGCAGC AGCAGCTGCT TAATCTTCGA 
CAAGAGCAAT ACTATAGCGA AAAATACCAG AAACGAGAAC ACATTAAACT GCTTATTCAA 
AAATTGACTG ATGTTGCTGA AGAATGTCAG AATAATCAGT TAAAGAAGCT CAAAGAAATC 
TGTGAGAAAG AGAAGAAGGA ATTAAAGAAG AAAATGGATA AAAAGAGGCA GGAGAAGATA 
ACAGAAGCAA AATCCAAAGA CAAAAGCCAG ATGGAGGAGG AAAAGACAGA GATGATCCGG 
TCATACATCC AGGAGGTGGT GCAGTACATC AAGAGGTTAG AAGAGGCGCA AAGTAAACGG 
CAAGAAAAAC TCGTAGAGAA ACACAAAGAA ATCCGCCAGC AGATCCTGGA TGAAAAGCCC 
AAGGGGGACG GTTCCTCCTC ATTCTTGTCG GAAACTTGCC ATGAGGATCC CTCTGTTTCC 
CCCAACTTTA CTCCCCCCAA CCCTCAAGCT CTCAAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005214112.1
CDS	45..3563
Translation	MAGAQPGVHALQLKPVCVSDSLKKGTKFVKWEDDSTVVTPIILRTDPQGFFFYWTDQNKETELLDLSLVKDARCGKHAKAPKDPKLRELLDVGNIGRLEHRMITVVYGPDLVNISHLNLVAFQEEVAKEWTNEVFSLATNLLAQNMSRDAFLEKAYTKLKLQVTPEGRIPLKNIYRLFSADRKRVETALEACSLPSSRNDSIPQEDFTPEVYRVFLNNLCPRPEIDNIFSEFGAKSKPYLTVDQMMDFINLKQRDPRLNEILYPPLKQEQVQVLIEKYEPNNSLAKKGQISVDGFMRYLSGEENGVVSPEKLDLNEDMSQPLSHYFINSSHNTYLTAGQLAGNSSVEMYRQVLLSGCRCVELDCWKGRTAEEEPVITHGFTMTTEISFKEVIEAIAECAFKTSPFPILLSFENHVDSPKQQAKMAEYCRLIFGDALLMEPLDKYPLESGVPLPSPMDLMYKILVKNKKKSHKSSEGSGKKKLSEQASNTYSDSSSVFEPSSPGAGEADTESDDDDDDDDCKKSSMDEGTAGSEAMATEEMSNLVNYIQPVKFESFEISKKRNRSFEMSSFVETKGLEQLTKSPVEFVEYNKMQLSRIYPKGTRVDSSNYMPQLFWNAGCQMVALNFQTVDLAMQINMGMYEYNGKSGYRLKPEFMRRPDKHFDPFTEGIVDGIVANTLSVKIISGQFLSDKKVGTYVEVDMFGLPVDTRRKAFKTKTSQGNAVNPIWEEEPIVFKKVVLPSLACLRIAVYEEGGKFIGHRILPVQAIRPGYHYICLRNERNQPLMLPALFVYIEVKDYVPDTYADVIEALSNPIRYVNLMEQRAKQLAALTLEDEEEVKKEADPGETPSEAPSEARPTPAENGVNHTTSLTPKPPSQALHSQPAPGSVKAPAKTEDLIQSVLTEVEAQTIEELKQQKSFVKLQKKHYKEMKDLVKRHHKKTTDLIKEHTTKYNEIQNDYLRRRAALEKTAKKDNKKKSEPSSPDHVSSTIEQDLAALDAEMTQKLVDLKDKQQQQLLNLRQEQYYSEKYQKREHIKLLIQKLTDVAEECQNNQLKKLKEICEKEKKELKKKMDKKRQEKITEAKSKDKSQMEEEKTEMIRSYIQEVVQYIKRLEEAQSKRQEKLVEKHKEIRQQILDEKPKGDGSSSFLSETCHEDPSVSPNFTPPNPQALK

Target ORF information:

RefSeq Version	XM_005214055.3
Organism	Bos taurus(cattle)
Definition	Bos taurus phospholipase C beta 1 (PLCB1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005214055.3

ORF Insert Sequence:

ATGGCCGGGG CACAGCCTGG AGTGCACGCG TTGCAACTCA AGCCGGTGTG CGTGTCCGAC 
AGCCTCAAGA AGGGCACCAA ATTCGTCAAG TGGGAGGATG ACTCCACTGT TGTCACTCCG 
ATTATTTTGA GGACTGACCC TCAGGGATTT TTCTTTTACT GGACAGATCA AAACAAGGAG 
ACGGAGCTGT TAGATCTCAG CCTTGTCAAA GACGCCAGAT GTGGGAAGCA TGCCAAGGCG 
CCCAAGGACC CTAAATTACG TGAACTTCTG GATGTGGGGA ACATTGGGCG CCTGGAACAT 
CGCATGATCA CAGTGGTGTA TGGGCCTGAC TTAGTGAACA TCTCCCATTT GAACCTCGTG 
GCTTTCCAAG AAGAAGTGGC CAAGGAATGG ACAAATGAGG TTTTCAGTTT GGCAACAAAC 
CTGCTGGCCC AAAACATGTC CAGGGATGCA TTTCTGGAAA AGGCGTATAC TAAGCTTAAG 
CTGCAAGTCA CTCCAGAAGG ACGCATTCCT CTCAAAAACA TATACCGCCT GTTCTCAGCA 
GACCGGAAGC GAGTCGAGAC AGCTTTAGAG GCTTGTAGTC TTCCATCTTC AAGGAATGAT 
TCGATACCTC AAGAAGATTT CACTCCTGAA GTGTACAGAG TTTTCCTGAA CAACCTTTGT 
CCTCGACCTG AAATTGATAA CATCTTTTCC GAATTTGGTG CCAAAAGCAA ACCATACCTT 
ACAGTTGATC AGATGATGGA TTTTATCAAC CTCAAGCAGC GAGATCCCCG GCTAAATGAA 
ATACTTTACC CACCTTTAAA GCAAGAGCAA GTCCAAGTGT TGATTGAGAA GTATGAACCT 
AACAACAGCC TCGCCAAAAA AGGACAGATA TCAGTGGATG GATTTATGCG CTATCTGAGT 
GGAGAAGAAA ATGGAGTCGT TTCACCTGAG AAACTGGATT TGAATGAAGA TATGTCTCAG 
CCCCTTTCTC ACTATTTCAT TAATTCCTCA CACAACACCT ACCTCACAGC TGGCCAGCTG 
GCTGGAAACT CCTCCGTTGA GATGTATCGC CAAGTGCTCC TGTCTGGTTG TCGCTGTGTG 
GAGCTGGACT GCTGGAAGGG ACGAACTGCA GAAGAGGAGC CAGTCATCAC CCATGGGTTC 
ACCATGACCA CAGAAATATC CTTCAAGGAA GTGATTGAAG CCATTGCTGA ATGTGCATTT 
AAGACTTCAC CTTTTCCAAT TCTGCTTTCA TTTGAGAACC ATGTGGACTC GCCGAAGCAG 
CAAGCCAAGA TGGCGGAGTA CTGCAGACTC ATCTTCGGCG ACGCCCTCCT CATGGAGCCG 
CTGGACAAGT ACCCGTTGGA ATCTGGAGTT CCCCTTCCAA GTCCTATGGA TTTAATGTAC 
AAAATTTTGG TGAAAAATAA GAAGAAATCA CACAAGTCGT CAGAAGGAAG TGGCAAGAAG 
AAGCTCTCAG AACAAGCTTC CAACACGTAC AGCGACTCCT CCAGTGTGTT TGAGCCCTCG 
TCCCCAGGAG CTGGAGAAGC TGATACAGAG AGTGACGATG ATGACGATGA TGATGACTGT 
AAAAAGTCTT CAATGGATGA GGGGACCGCA GGGAGCGAGG CCATGGCCAC AGAAGAGATG 
TCTAATCTGG TGAACTATAT CCAGCCAGTC AAGTTCGAGT CATTTGAAAT TTCAAAAAAA 
AGAAATAGAA GCTTCGAGAT GTCTTCCTTT GTGGAAACCA AAGGTCTTGA GCAACTTACA 
AAGTCTCCGG TGGAATTTGT AGAATATAAC AAAATGCAGC TTAGCAGGAT ATATCCAAAG 
GGAACACGCG TGGATTCATC TAACTATATG CCTCAGCTCT TCTGGAATGC AGGCTGCCAA 
ATGGTTGCTC TTAATTTCCA AACAGTGGAC CTGGCTATGC AGATAAACAT GGGGATGTAT 
GAATATAATG GGAAGAGTGG CTACAGGCTG AAGCCGGAGT TCATGAGGAG ACCTGACAAG 
CACTTTGATC CATTCACAGA AGGCATCGTG GACGGCATAG TGGCCAACAC TTTATCTGTT 
AAGATTATTT CAGGTCAGTT TCTCTCTGAT AAGAAAGTCG GGACTTATGT GGAAGTGGAT 
ATGTTTGGTT TGCCTGTGGA CACAAGGAGG AAGGCATTTA AGACCAAAAC ATCACAAGGA 
AACGCTGTAA ATCCCATCTG GGAAGAAGAA CCCATTGTAT TCAAAAAGGT GGTTCTTCCT 
TCGCTAGCCT GTCTGAGGAT AGCAGTTTAT GAAGAAGGAG GTAAATTTAT TGGTCACCGA 
ATCTTGCCAG TACAAGCAAT TCGGCCAGGC TATCACTACA TCTGTCTGAG GAATGAGAGG 
AACCAGCCTC TCATGCTGCC AGCGCTCTTT GTCTACATAG AGGTGAAGGA CTATGTCCCA 
GACACATATG CAGATGTGAT TGAAGCTTTG TCAAATCCAA TCCGATATGT GAATCTGATG 
GAGCAGAGAG CTAAACAACT GGCTGCTTTG ACACTGGAAG ATGAAGAAGA AGTGAAGAAA 
GAGGCTGATC CTGGGGAAAC ACCATCAGAA GCTCCGAGTG AAGCCAGGCC AACTCCAGCA 
GAAAACGGGG TGAATCACAC AACGTCCCTG ACACCCAAAC CACCCTCCCA GGCTCTGCAC 
AGCCAGCCAG CTCCAGGTTC TGTAAAGGCA CCTGCCAAAA CAGAAGATCT TATTCAGAGT 
GTTTTAACAG AAGTAGAAGC GCAGACGATT GAAGAGCTAA AGCAGCAGAA GTCGTTTGTG 
AAACTTCAAA AGAAGCACTA CAAGGAAATG AAGGACCTGG TTAAGAGACA CCACAAGAAA 
ACCACCGACC TCATCAAAGA GCACACTACA AAATACAATG AAATCCAGAA TGACTACTTG 
AGAAGGAGAG CAGCTCTGGA AAAGACAGCC AAAAAGGACA ATAAGAAAAA ATCAGAACCC 
AGTAGCCCTG ACCACGTCTC ATCAACAATT GAGCAAGACC TTGCTGCCCT GGATGCCGAG 
ATGACCCAAA AGTTAGTAGA TCTGAAGGAC AAACAGCAGC AGCAGCTGCT TAATCTTCGA 
CAAGAGCAAT ACTATAGCGA AAAATACCAG AAACGAGAAC ACATTAAACT GCTTATTCAA 
AAATTGACTG ATGTTGCTGA AGAATGTCAG AATAATCAGT TAAAGAAGCT CAAAGAAATC 
TGTGAGAAAG AGAAGAAGGA ATTAAAGAAG AAAATGGATA AAAAGAGGCA GGAGAAGATA 
ACAGAAGCAA AATCCAAAGA CAAAAGCCAG ATGGAGGAGG AAAAGACAGA GATGATCCGG 
TCATACATCC AGGAGGTGGT GCAGTACATC AAGAGGTTAG AAGAGGCGCA AAGTAAACGG 
CAAGAAAAAC TCGTAGAGAA ACACAAAGAA ATCCGCCAGC AGATCCTGGA TGAAAAGCCC 
AAGGGGGACG GTTCCTCCTC ATTCTTGTCG GAAACTTGCC ATGAGGATCC CTCTGTTTCC 
CCCAACTTTA CTCCCCCCAA CCCTCAAGCT CTCAAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

A whole-genome assembly of the domestic cow, Bos taurus.
Genome biology10(4)R42(2009)
Zimin AV,Delcher AL,Florea L,Kelley DR,Schatz MC,Puiu D,Hanrahan F,Pertea G,Van Tassell CP,Sonstegard TS,Marçais G,Roberts M,Subramanian P,Yorke JA,Salzberg SL

Concentration of enzyme-dependent activation of PLC-beta 1 and PLC-beta 2 by G alpha 11 and beta gamma-subunits.
Cellular signalling7(7)709-20(1995 Sep)
Paterson A,Boyer JL,Watts VJ,Morris AJ,Price EM,Harden TK

Determination of the primary structure of PLC-154 demonstrates diversity of phosphoinositide-specific phospholipase C activities.
Cell54(2)171-7(1988 Jul)
Katan M,Kriz RW,Totty N,Philp R,Meldrum E,Aldape RA,Knopf JL,Parker PJ

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PLCB1 cDNA ORF clone, Bos taurus(cattle)

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