itgae cDNA ORF clone, Astyanax mexicanus(Mexican tetra)

Gene
Clones
gRNAs

Summary

Gene Symbol	itgae
Entrez Gene ID	103040037
Full Name	integrin subunit alpha E
Gene Type	protein-coding
Organism	Astyanax mexicanus(Mexican tetra)

mRNA and Protein(s)

mRNA	Protein	Name
XM_015608558.1	XP_015464044.1	integrin alpha-E-like
XM_022677873.1	XP_022533594.1	integrin alpha-E

Pathways from BioSystems
Gene Ontology

The following itgae gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the itgae cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OAs15939	XM_015608558.1 Latest version!	Astyanax mexicanus integrin alpha-E-like (LOC103040037), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
OAs122407	XM_022677873.1 Latest version!	Astyanax mexicanus integrin subunit alpha E (itgae), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

itgae ( XM_015608558.1 ) cDNA ORF clone, Astyanax mexicanus(Mexican tetra) -> XP_015464044.1 Astyanax mexicanus integrin alpha-E-like (LOC103040037), mRNA.

Price & Availability↑

CloneID	OAs15939
Clone ID Related Accession (Same CDS sequence)	XM_015608558.1
Accession Version	XM_015608558.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2952bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1455033600000
Organism	Astyanax mexicanus(Mexican tetra)
Product	integrin alpha-E-like
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_006738844.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Astyanax mexicanus Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## ab initio :: 1% of CDS bases ##RefSeq-Attributes-END##

ATGAAGTATC AGTCACTTCA GTTGGATTAT CTCAGCAAGA TGTTTGCAGC ATTTCTCCTG 
GTGACAGGGC TATGCACCAC GGCAGCATTC AACATATTCA CAACGCAGGT AGAGACCTTT 
ACAAACATTG ATCCACGGTT TGGACAGACC ATAGTCAAGT CAAGAGATGG AGTTTACGTC 
CCTACCAAAG GAAAGGTTTT CAAATGTGTG CAGGACAGTG AGTGTGTTCT GGTGAATGAA 
ATCGAAATGA AAGGCCTGAA ACCAATCGTA TCTGTATCAT CCAACTTGAC TGAGGAGGAA 
GAGCAATTCC TGGTTTGTAA TCAAGTTCGG ACCCGGAACG GCTCTGCTGA GTATTTCAAT 
GGACTTTGTG TGCACATGAT TAATGAGGAT AAGCAGGCTG ACATCAATCC TTCCAAGTTA 
GTCTCACAGC AACAGCAAAA AAATGCTTTG AAGGGTGCCA GTGAGAATAA AACAGGTCAC 
TCAAGGCGGA AAAGGCAGGC TGAGCTAAAT GACGATGACG AAGAAGAAGA TGAAGAAGAA 
GACGCTGGGA CGGAGATTGC TTTTGTACTG GATGGTTCTG GGAGCATTGA ACCTGAGGAT 
TTTGTTCGGG CCAAAGACTT TATGTCTGAT GTGATGAAAA ATGTTTGGAC GACCTGTTTA 
ACATGTAATT TTGCAATAGT GCAGTTCGGA TCTGAAATAA GGACAGAACT CTCATTGATG 
GATAATGATG ATGGAGAAAA GGCCTTGGAT AAGGTGAAAA ACATAAACCA GATAGGCAAG 
CTGACCAAGA CTGCCTCAGC CCTCTACCAT GTTCTGACAG AAGTGTTTGT TCCTGAACAC 
GGGTCAAAAC CGAACGCCAC AAAAATGATT ATCGTATTAT CTGACGGAGA AATGAAAGGA 
ATCCAGCCGA GGAATCTCTC ATATGTCTTG GAAATGCCGC AGATGAAAGG AGTTCTTCGC 
TATTCCATAG GGGTTGGATC TGAGCTTCTG AACAGTAAAA AAGCAATAAA TGAGATGACG 
GAGATAGCTG GTAGCAAAGA CAGATTCTTT AAGGTTTCTG ATTACGCAGC CTTGAACACA 
ATTCTTTCTA AAATAGAAAA AAGCATATTT GACATTGAAG GCATTAATAA GGGTGCAGGA 
TTTCATTTTG AACTTGCAGA AGCTGGATTC AGCTCTCATC TAACTCACGA TGGGTCAATG 
CTGTTTGGGG CGGTGGGGGC CTATGACTGG AGTGGGGGAA TTATCCTGAA AGGGAAGGAA 
GAAGAGAGTG TGACATTTTT TAATGCAACT ACTGAAGAAC CGAGGTTTTC TTATTTGGGT 
TACAGTGTGA CATCTGGACG CATGGGTAAT GAAAACCTAT ACATATCTGG AGCTCCCAGG 
TACAACTTGA CTGGTGCAGT CTTTATCTTT AATGGAACCG ATCAGGAGTT GATTCTAGGA 
GATCAGGTTG GATCTTACTT TGGATCAGTT CTTTGTGCCT TGGACATTGA TAGCGATCAG 
GAGACAGATT ACCTGCTGGT TGGAGCCCCA CACTTTCATA TAAAAGGGGA AGAAGGCAAA 
GTGTTGGTGT ACAAACTTCA TGAAGGGAAG TTTCAGAGAG AAGATTATGT TCTGCAAGGA 
ACAGGGAAAC ACATCTACGC CAGGTTTGGC TCTGCCATAG TCGATATTGG AGATATTGAT 
GGGAACAAAT TCAGAGACGT GGCAGTGGGA GCTCCTCTGG AACCAGCAGA TGAGCTGACT 
GATGTTTCCG GAGCCATTTA CATCTATAAC GGATTTGAAT ATGGGATAAA GCAGCAGTTT 
TCACAGAGAA TTGCAGCTTC TGGTTTTGAG ATGAAACTTG TACATTTTGG TCAGGCAGTG 
AGTGCCATGA TGAAGAAAGA AGATTCAGAA AGAGACAAGA ACAATCTTTT ACTCAGTGTT 
GGGAGTCAAG GGGCTGTCAC TGTATTAATT GAGTTCCAAA AGGAGTGTAA TATCCCAACA 
AGCTGCCAGC CAAAATTTTC ATTAGCTGGT TCAAAACTAA GCAATGACTT GATCATAATT 
GGATCCTCTC AATCCTTGGG TATAATCTTT GATCTTCAAA ACTCTGGGGA AAGCTCGTAT 
ATCACAACTC TGACTCTGAC TTATCCTAAC ATACTACAGG CAAAGAAAAT TGAGGGCGGA 
TCATGTGAGA TGAAAAATGA GGACCTTCAA ATAATATGCA AACTCTTACA TCCTGTTTTT 
AAGGGAGGTG CACAGACCAA GGTCGCAATC GACTGGCAGC CGATTAACAG CAGAAAGTCA 
GACCTGACCA CTGGGACGAT CAGAGCTGTT TTAACAGGAG GAAATGCTGG CACTGAGACC 
CTGGCCTCTA AGGAGTATGA TTTTGTAGTG AGGAATGCCC TAAAACTTCA GCTCACTGGC 
ACAGCTTCAC AAAACATTTT CGCGATTGAA GAAGGAAAAG AAAGCCCAAA TAAGGACCCA 
AAAGTGCTGC AGTTCACTTT TAAGCTACTG GGTGACAACA AGTACAAGGC TGAAATACAT 
GTGATGATAA CAATAGAAAA GAAAACACAA GACATAAAAC TGATCATCAA GCAAGTTCGA 
CCTCAAGAGC ATTGTAAAAC ACTGTCTGGA GAACAGAACG AGAGCAACCT TCGTGTAGAA 
TGCATTCTGA CTGAATTGCA AGACATTACC ATCAACACTG ATGTCATTAT TAATGACATT 
AAGATTACTG AGAGGATCAC TGCCAAAGCA GAACTCAGCT TTAATGAGAG CATCTACGAA 
GGACAAGACA TGAGGAAGAT GGAAAAGGTG GAAGTGGTAG TTATCAAGCA GAAGATTGTG 
AAATCAACTG CAGTAATTGC AGGTGGCTCC GTTGGGGGGT TTCTGCTGTT AATCATCATC 
ATCATTGTTC TTATAAAGTG TGGCTTCTTC CGTCGACGGC ATCATATGGA GCGTACACAG 
AGCACACACT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015464044.1
CDS	57..3008
Translation	MKYQSLQLDYLSKMFAAFLLVTGLCTTAAFNIFTTQVETFTNIDPRFGQTIVKSRDGVYVPTKGKVFKCVQDSECVLVNEIEMKGLKPIVSVSSNLTEEEEQFLVCNQVRTRNGSAEYFNGLCVHMINEDKQADINPSKLVSQQQQKNALKGASENKTGHSRRKRQAELNDDDEEEDEEEDAGTEIAFVLDGSGSIEPEDFVRAKDFMSDVMKNVWTTCLTCNFAIVQFGSEIRTELSLMDNDDGEKALDKVKNINQIGKLTKTASALYHVLTEVFVPEHGSKPNATKMIIVLSDGEMKGIQPRNLSYVLEMPQMKGVLRYSIGVGSELLNSKKAINEMTEIAGSKDRFFKVSDYAALNTILSKIEKSIFDIEGINKGAGFHFELAEAGFSSHLTHDGSMLFGAVGAYDWSGGIILKGKEEESVTFFNATTEEPRFSYLGYSVTSGRMGNENLYISGAPRYNLTGAVFIFNGTDQELILGDQVGSYFGSVLCALDIDSDQETDYLLVGAPHFHIKGEEGKVLVYKLHEGKFQREDYVLQGTGKHIYARFGSAIVDIGDIDGNKFRDVAVGAPLEPADELTDVSGAIYIYNGFEYGIKQQFSQRIAASGFEMKLVHFGQAVSAMMKKEDSERDKNNLLLSVGSQGAVTVLIEFQKECNIPTSCQPKFSLAGSKLSNDLIIIGSSQSLGIIFDLQNSGESSYITTLTLTYPNILQAKKIEGGSCEMKNEDLQIICKLLHPVFKGGAQTKVAIDWQPINSRKSDLTTGTIRAVLTGGNAGTETLASKEYDFVVRNALKLQLTGTASQNIFAIEEGKESPNKDPKVLQFTFKLLGDNKYKAEIHVMITIEKKTQDIKLIIKQVRPQEHCKTLSGEQNESNLRVECILTELQDITINTDVIINDIKITERITAKAELSFNESIYEGQDMRKMEKVEVVVIKQKIVKSTAVIAGGSVGGFLLLIIIIIVLIKCGFFRRRHHMERTQSTH

Target ORF information:

RefSeq Version	XM_015608558.1
Organism	Astyanax mexicanus(Mexican tetra)
Definition	Astyanax mexicanus integrin alpha-E-like (LOC103040037), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015608558.1

ORF Insert Sequence:

ATGAAGTATC AGTCACTTCA GTTGGATTAT CTCAGCAAGA TGTTTGCAGC ATTTCTCCTG 
GTGACAGGGC TATGCACCAC GGCAGCATTC AACATATTCA CAACGCAGGT AGAGACCTTT 
ACAAACATTG ATCCACGGTT TGGACAGACC ATAGTCAAGT CAAGAGATGG AGTTTACGTC 
CCTACCAAAG GAAAGGTTTT CAAATGTGTG CAGGACAGTG AGTGTGTTCT GGTGAATGAA 
ATCGAAATGA AAGGCCTGAA ACCAATCGTA TCTGTATCAT CCAACTTGAC TGAGGAGGAA 
GAGCAATTCC TGGTTTGTAA TCAAGTTCGG ACCCGGAACG GCTCTGCTGA GTATTTCAAT 
GGACTTTGTG TGCACATGAT TAATGAGGAT AAGCAGGCTG ACATCAATCC TTCCAAGTTA 
GTCTCACAGC AACAGCAAAA AAATGCTTTG AAGGGTGCCA GTGAGAATAA AACAGGTCAC 
TCAAGGCGGA AAAGGCAGGC TGAGCTAAAT GACGATGACG AAGAAGAAGA TGAAGAAGAA 
GACGCTGGGA CGGAGATTGC TTTTGTACTG GATGGTTCTG GGAGCATTGA ACCTGAGGAT 
TTTGTTCGGG CCAAAGACTT TATGTCTGAT GTGATGAAAA ATGTTTGGAC GACCTGTTTA 
ACATGTAATT TTGCAATAGT GCAGTTCGGA TCTGAAATAA GGACAGAACT CTCATTGATG 
GATAATGATG ATGGAGAAAA GGCCTTGGAT AAGGTGAAAA ACATAAACCA GATAGGCAAG 
CTGACCAAGA CTGCCTCAGC CCTCTACCAT GTTCTGACAG AAGTGTTTGT TCCTGAACAC 
GGGTCAAAAC CGAACGCCAC AAAAATGATT ATCGTATTAT CTGACGGAGA AATGAAAGGA 
ATCCAGCCGA GGAATCTCTC ATATGTCTTG GAAATGCCGC AGATGAAAGG AGTTCTTCGC 
TATTCCATAG GGGTTGGATC TGAGCTTCTG AACAGTAAAA AAGCAATAAA TGAGATGACG 
GAGATAGCTG GTAGCAAAGA CAGATTCTTT AAGGTTTCTG ATTACGCAGC CTTGAACACA 
ATTCTTTCTA AAATAGAAAA AAGCATATTT GACATTGAAG GCATTAATAA GGGTGCAGGA 
TTTCATTTTG AACTTGCAGA AGCTGGATTC AGCTCTCATC TAACTCACGA TGGGTCAATG 
CTGTTTGGGG CGGTGGGGGC CTATGACTGG AGTGGGGGAA TTATCCTGAA AGGGAAGGAA 
GAAGAGAGTG TGACATTTTT TAATGCAACT ACTGAAGAAC CGAGGTTTTC TTATTTGGGT 
TACAGTGTGA CATCTGGACG CATGGGTAAT GAAAACCTAT ACATATCTGG AGCTCCCAGG 
TACAACTTGA CTGGTGCAGT CTTTATCTTT AATGGAACCG ATCAGGAGTT GATTCTAGGA 
GATCAGGTTG GATCTTACTT TGGATCAGTT CTTTGTGCCT TGGACATTGA TAGCGATCAG 
GAGACAGATT ACCTGCTGGT TGGAGCCCCA CACTTTCATA TAAAAGGGGA AGAAGGCAAA 
GTGTTGGTGT ACAAACTTCA TGAAGGGAAG TTTCAGAGAG AAGATTATGT TCTGCAAGGA 
ACAGGGAAAC ACATCTACGC CAGGTTTGGC TCTGCCATAG TCGATATTGG AGATATTGAT 
GGGAACAAAT TCAGAGACGT GGCAGTGGGA GCTCCTCTGG AACCAGCAGA TGAGCTGACT 
GATGTTTCCG GAGCCATTTA CATCTATAAC GGATTTGAAT ATGGGATAAA GCAGCAGTTT 
TCACAGAGAA TTGCAGCTTC TGGTTTTGAG ATGAAACTTG TACATTTTGG TCAGGCAGTG 
AGTGCCATGA TGAAGAAAGA AGATTCAGAA AGAGACAAGA ACAATCTTTT ACTCAGTGTT 
GGGAGTCAAG GGGCTGTCAC TGTATTAATT GAGTTCCAAA AGGAGTGTAA TATCCCAACA 
AGCTGCCAGC CAAAATTTTC ATTAGCTGGT TCAAAACTAA GCAATGACTT GATCATAATT 
GGATCCTCTC AATCCTTGGG TATAATCTTT GATCTTCAAA ACTCTGGGGA AAGCTCGTAT 
ATCACAACTC TGACTCTGAC TTATCCTAAC ATACTACAGG CAAAGAAAAT TGAGGGCGGA 
TCATGTGAGA TGAAAAATGA GGACCTTCAA ATAATATGCA AACTCTTACA TCCTGTTTTT 
AAGGGAGGTG CACAGACCAA GGTCGCAATC GACTGGCAGC CGATTAACAG CAGAAAGTCA 
GACCTGACCA CTGGGACGAT CAGAGCTGTT TTAACAGGAG GAAATGCTGG CACTGAGACC 
CTGGCCTCTA AGGAGTATGA TTTTGTAGTG AGGAATGCCC TAAAACTTCA GCTCACTGGC 
ACAGCTTCAC AAAACATTTT CGCGATTGAA GAAGGAAAAG AAAGCCCAAA TAAGGACCCA 
AAAGTGCTGC AGTTCACTTT TAAGCTACTG GGTGACAACA AGTACAAGGC TGAAATACAT 
GTGATGATAA CAATAGAAAA GAAAACACAA GACATAAAAC TGATCATCAA GCAAGTTCGA 
CCTCAAGAGC ATTGTAAAAC ACTGTCTGGA GAACAGAACG AGAGCAACCT TCGTGTAGAA 
TGCATTCTGA CTGAATTGCA AGACATTACC ATCAACACTG ATGTCATTAT TAATGACATT 
AAGATTACTG AGAGGATCAC TGCCAAAGCA GAACTCAGCT TTAATGAGAG CATCTACGAA 
GGACAAGACA TGAGGAAGAT GGAAAAGGTG GAAGTGGTAG TTATCAAGCA GAAGATTGTG 
AAATCAACTG CAGTAATTGC AGGTGGCTCC GTTGGGGGGT TTCTGCTGTT AATCATCATC 
ATCATTGTTC TTATAAAGTG TGGCTTCTTC CGTCGACGGC ATCATATGGA GCGTACACAG 
AGCACACACT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

itgae ( XM_022677873.1 ) cDNA ORF clone, Astyanax mexicanus(Mexican tetra) -> XP_022533594.1 Astyanax mexicanus integrin subunit alpha E (itgae), mRNA.

Price & Availability↑

CloneID	OAs122407
Clone ID Related Accession (Same CDS sequence)	XM_022677873.1
Accession Version	XM_022677873.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3375bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1506873600000
Organism	Astyanax mexicanus(Mexican tetra)
Product	integrin alpha-E
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_035898.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Astyanax mexicanus Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## ab initio :: 1% of CDS bases ##RefSeq-Attributes-END##

ATGAAGTATC AGTCACTTCA GTTGGATTAT CTCAGCAAGA TGTTTGCAGC ATTTCTCCTG 
GTGACAGGGC TATGCACCAC GGCAGCATTC AACATATTCA CAACGCAGGT AGAGACCTTT 
ACAAACATTG ATCCACGGTT TGGACAGACC ATAGTCAAGT CAAGAGATGG AGTTTACGTC 
CCTACCAAAG GAAAGGTTTT CAAATGTGTG CAGGACAGTG AGTGTGTTCT GGTGAATGAA 
ATCGAAATGA AAGGCCTGAA ACCAATCGTA TCTGTATCAT CCAACTTGAC TGAGGAGGAA 
GAGCAATTCC TGGTTTGTAA TCAAGTTCGG ACCCGGAACG GCTCTGCTGA GTATTTCAAT 
GGACTTTGTG TGCACATGAT TAATGAGGAT AAGCAGGCTG ACATCAATCC TTCCAAGTTA 
GTCTCACAGC AACAGCAAAA AAATGCTTTG AAGGGTGCCA GTGAGAATAA AACAGGTCAC 
TCAAGGCGGA AAAGGCAGGC TGAGCTAAAT GGCGATGACG AAGAAGAAGA TGAAGAAGAA 
GACGCTGGGA CGGAGATTGC TTTTGTACTG GATGGTTCTG GGAGCATTGA ACCTGAGGAT 
TTTGTTCGGG CCAAAGACTT TATGTCTGAT GTGATGAAAA ATGTTTGGAC GACCTGTTTA 
ACATGTAATT TTGCAATAGT GCAGTTCGGA TCTGAAATAA GGACAGAACT CTCATTGATG 
GATAATGATG ATGGAGAAAA GGCCTTGGAT AAGGTGAAAA ACATAAACCA GATAGGCAAG 
CTGACCAAGA CTGCCTCAGC CCTCTACCAT GTTCTGACAG AAGTGTTTGT TCCTGAACAC 
GGGTCAAAAC CGAACGCCAC AAAAATGATT ATCGTATTAT CTGACGGAGA AATGAAAGGA 
ATCCAGCCGA GGAATCTCTC ATATGTCTTG GAAATGCCGC AGATGAAAGG AGTTCTTCGC 
TATTCCATAG GGGTTGGATC TGAGCTTCTG AACAGTAAAA AAGCAATAAA TGAGATGACG 
GAGATAGCTG GTAGCAAAGA CAGATTCTTT AAGGTTTCTG ATTACGCAGC CTTGAACACA 
ATTCTTTCTA AAATAGAAAA AAGCATATTT GACATTGAAG GCATTAATAA GGGTGCAGGA 
TTTCATTTTG AACTTGCAGA AGCTGGATTC AGCTCTCATC TAACTCACGA AGGGTCAATG 
CTGTTTGGGG CGGTGGGGGC CTATGACTGG AGTGGGGGAA TTATCCTGAA AGGGAAGGAA 
GAAGAGAGTG TGACATTTTT TAATGCAACT ACTGAAGAAC CGAGGTTTTC TTATTTGGGT 
TACAGTGTGA CATCTGGACG CATGGGTAAT GAAAACCTAT ACATATCTGG AGCTCCCAGG 
TACAACTTGA CTGGTGCAGT CTTTATCTTT AATGGAACCG ATCAGGAGTT GATTCTAGGA 
GATCAGGTTG GATCTTACTT TGGATCAGTT CTTTGTGCCT TGGACATTGA TAGCGATCAG 
GAGACAGATT ACCTGCTGGT TGGAGCCCCA CACTTTCATA TAAAAGGGGA AGAAGGCAAA 
GTGTTTGTGT ACAAACTTCA TGAAGGGAAG TTTCAGAGAG AAGATTATGT ACTGCAAGGA 
ACAGGGAAAC ACATCTACGC CAGGTTTGGC TCTGCCATAG TCGATATTGG AGATATTGAT 
GGGAACAAAT TCAGAGACGT GGCAGTGGGA GCTCCTCTGG AACCAGCAGA TGAGCTGACT 
GATGTTTCCG GAGCCATTTA CATCTATAAC GGATTTGAAT ATGGGATAAA GCAGCAGTTT 
TCACAGAGAA TTGCAGCTTC TGGTTTTGAG ATGAAACTTG TACATTTTGG TCAGGCAGTG 
AGTGCCATGA TGAAGAAAGA AGATTCAGAA AGAGACAAGA ACAATCTTTT ACTCAGTGTT 
GGGAGTCAAG GGGCTGTCAC TGTATTAAAG ACAGTTCCTG TCATCATTAT CACACCAAAA 
ATAACACTTG GTCTAACAGA GATTTCCCCT GATAAGCAGA ACAATGAAAA ATCAAGCCAA 
ATCGAGTCAT TTATCCAAAT CTGCTTTGAT AACACAAGGA AAAGAATTGA CAATGATGAA 
GAACTACTCA TTGAGTATCA AATTGATTTG GACTTCGGTA AAGAAGAAAA ACGACTAATT 
TTTGTTGAAC ACCATGAAAA GCAAACCAGA TTCACTCTGA CAAAATATAA AAACTGCATA 
AGCAAAATAA ATGTTAAATA TATGGGCTGC TATGACTGTT TTTCACCTAT AAAAATAAAG 
GTACAATTTG AATTGCCCTC ACACACTGAG GGAATCCCTG TGCGAGTGCT TGATGCTTTT 
AGTCCAACGG AGTTCACAAA AGAGATTGAG TTCCAAAAGG AGTGTAATAT CACAACAAGC 
TGCAAGCCAA AATTTTCATT AGATGGTTCA AAACTAAGTT ACAGCAATTA CTTGATCATA 
ATTGGATCCT CTCAATCCTT GGGTATAATC TTTGATCTTC AAAACTCTGG GAAAAGCTCG 
TATATCACAA CTCTGACTCT GACTTATCCT AACATACTAC AGGCAAAGAA AATTGAGGGC 
GGATCATGTG AGATGAAAAA TGAGGACCTT CAAATAATAT GCAAACTCTT ACATCCTGTT 
TTTAAGGGAG GTGCACAGAC CAAGGTCGCA ATCGACTGGC AGCCGATTAA CAGCAGAAAG 
TCAGACCTGA CCACTGGGAC GATCAGAGCT GTTTTAACAG GAGGAAATGC TGGCACTAAG 
ACCCTGGCCT CTAAGGAGTA TGATTTTGTA GTGAGGAATG CCCTAAAACT TCAGCTCACT 
GGCACAGCTT CACAAAACAT TTTCGCGATT GAAGAAGGAA AAGAAAGCCC AAATAAGGAC 
CCAAAAGTGC TGCAGTTCAC TTTTAAGCTA CTGGGTGACA ACAAGTACAA GGCTGAAATA 
CATGTGATGA TAACAATAGA AAAGAAAACA CAAGACATAA AACTGATCAT CAAGCAAGTT 
CGACCTCAAG AGCATTGTAA AACACTGTCT GGAGAACAGA ACGAGAGCAA CCTTCGTGTA 
GAATGCATTC TGACTGAATT GCAAGACATT ACCATCAACA CTGATGTCAT TATTAATGAC 
ATTAAGATTA CTGAGAGGAT CACTGCCAAA GCAGAACTCA GCTTTAATGA GAGCATCTAC 
GAAGGACAAG ACATGAGGAA GATGGAAAAG GTGGAAGTGG TAGTTATCAA GCAGAAGATT 
GTGAAATCAA CTGCAGTAAT TGCAGGTGGC TCCGTTGGGG GGTTTCTGCT GTTAATCATC 
ATCATCATTG TTCTTATAAA GTGTGGCTTC TTCCGTCGAC GGCATCATAT GGAGCGTACA 
CAGAGCACAC ACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_022533594.1
CDS	64..3438
Translation	MKYQSLQLDYLSKMFAAFLLVTGLCTTAAFNIFTTQVETFTNIDPRFGQTIVKSRDGVYVPTKGKVFKCVQDSECVLVNEIEMKGLKPIVSVSSNLTEEEEQFLVCNQVRTRNGSAEYFNGLCVHMINEDKQADINPSKLVSQQQQKNALKGASENKTGHSRRKRQAELNGDDEEEDEEEDAGTEIAFVLDGSGSIEPEDFVRAKDFMSDVMKNVWTTCLTCNFAIVQFGSEIRTELSLMDNDDGEKALDKVKNINQIGKLTKTASALYHVLTEVFVPEHGSKPNATKMIIVLSDGEMKGIQPRNLSYVLEMPQMKGVLRYSIGVGSELLNSKKAINEMTEIAGSKDRFFKVSDYAALNTILSKIEKSIFDIEGINKGAGFHFELAEAGFSSHLTHEGSMLFGAVGAYDWSGGIILKGKEEESVTFFNATTEEPRFSYLGYSVTSGRMGNENLYISGAPRYNLTGAVFIFNGTDQELILGDQVGSYFGSVLCALDIDSDQETDYLLVGAPHFHIKGEEGKVFVYKLHEGKFQREDYVLQGTGKHIYARFGSAIVDIGDIDGNKFRDVAVGAPLEPADELTDVSGAIYIYNGFEYGIKQQFSQRIAASGFEMKLVHFGQAVSAMMKKEDSERDKNNLLLSVGSQGAVTVLKTVPVIIITPKITLGLTEISPDKQNNEKSSQIESFIQICFDNTRKRIDNDEELLIEYQIDLDFGKEEKRLIFVEHHEKQTRFTLTKYKNCISKINVKYMGCYDCFSPIKIKVQFELPSHTEGIPVRVLDAFSPTEFTKEIEFQKECNITTSCKPKFSLDGSKLSYSNYLIIIGSSQSLGIIFDLQNSGKSSYITTLTLTYPNILQAKKIEGGSCEMKNEDLQIICKLLHPVFKGGAQTKVAIDWQPINSRKSDLTTGTIRAVLTGGNAGTKTLASKEYDFVVRNALKLQLTGTASQNIFAIEEGKESPNKDPKVLQFTFKLLGDNKYKAEIHVMITIEKKTQDIKLIIKQVRPQEHCKTLSGEQNESNLRVECILTELQDITINTDVIINDIKITERITAKAELSFNESIYEGQDMRKMEKVEVVVIKQKIVKSTAVIAGGSVGGFLLLIIIIIVLIKCGFFRRRHHMERTQSTH

Target ORF information:

RefSeq Version	XM_022677873.1
Organism	Astyanax mexicanus(Mexican tetra)
Definition	Astyanax mexicanus integrin subunit alpha E (itgae), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_022677873.1

ORF Insert Sequence:

ATGAAGTATC AGTCACTTCA GTTGGATTAT CTCAGCAAGA TGTTTGCAGC ATTTCTCCTG 
GTGACAGGGC TATGCACCAC GGCAGCATTC AACATATTCA CAACGCAGGT AGAGACCTTT 
ACAAACATTG ATCCACGGTT TGGACAGACC ATAGTCAAGT CAAGAGATGG AGTTTACGTC 
CCTACCAAAG GAAAGGTTTT CAAATGTGTG CAGGACAGTG AGTGTGTTCT GGTGAATGAA 
ATCGAAATGA AAGGCCTGAA ACCAATCGTA TCTGTATCAT CCAACTTGAC TGAGGAGGAA 
GAGCAATTCC TGGTTTGTAA TCAAGTTCGG ACCCGGAACG GCTCTGCTGA GTATTTCAAT 
GGACTTTGTG TGCACATGAT TAATGAGGAT AAGCAGGCTG ACATCAATCC TTCCAAGTTA 
GTCTCACAGC AACAGCAAAA AAATGCTTTG AAGGGTGCCA GTGAGAATAA AACAGGTCAC 
TCAAGGCGGA AAAGGCAGGC TGAGCTAAAT GGCGATGACG AAGAAGAAGA TGAAGAAGAA 
GACGCTGGGA CGGAGATTGC TTTTGTACTG GATGGTTCTG GGAGCATTGA ACCTGAGGAT 
TTTGTTCGGG CCAAAGACTT TATGTCTGAT GTGATGAAAA ATGTTTGGAC GACCTGTTTA 
ACATGTAATT TTGCAATAGT GCAGTTCGGA TCTGAAATAA GGACAGAACT CTCATTGATG 
GATAATGATG ATGGAGAAAA GGCCTTGGAT AAGGTGAAAA ACATAAACCA GATAGGCAAG 
CTGACCAAGA CTGCCTCAGC CCTCTACCAT GTTCTGACAG AAGTGTTTGT TCCTGAACAC 
GGGTCAAAAC CGAACGCCAC AAAAATGATT ATCGTATTAT CTGACGGAGA AATGAAAGGA 
ATCCAGCCGA GGAATCTCTC ATATGTCTTG GAAATGCCGC AGATGAAAGG AGTTCTTCGC 
TATTCCATAG GGGTTGGATC TGAGCTTCTG AACAGTAAAA AAGCAATAAA TGAGATGACG 
GAGATAGCTG GTAGCAAAGA CAGATTCTTT AAGGTTTCTG ATTACGCAGC CTTGAACACA 
ATTCTTTCTA AAATAGAAAA AAGCATATTT GACATTGAAG GCATTAATAA GGGTGCAGGA 
TTTCATTTTG AACTTGCAGA AGCTGGATTC AGCTCTCATC TAACTCACGA AGGGTCAATG 
CTGTTTGGGG CGGTGGGGGC CTATGACTGG AGTGGGGGAA TTATCCTGAA AGGGAAGGAA 
GAAGAGAGTG TGACATTTTT TAATGCAACT ACTGAAGAAC CGAGGTTTTC TTATTTGGGT 
TACAGTGTGA CATCTGGACG CATGGGTAAT GAAAACCTAT ACATATCTGG AGCTCCCAGG 
TACAACTTGA CTGGTGCAGT CTTTATCTTT AATGGAACCG ATCAGGAGTT GATTCTAGGA 
GATCAGGTTG GATCTTACTT TGGATCAGTT CTTTGTGCCT TGGACATTGA TAGCGATCAG 
GAGACAGATT ACCTGCTGGT TGGAGCCCCA CACTTTCATA TAAAAGGGGA AGAAGGCAAA 
GTGTTTGTGT ACAAACTTCA TGAAGGGAAG TTTCAGAGAG AAGATTATGT ACTGCAAGGA 
ACAGGGAAAC ACATCTACGC CAGGTTTGGC TCTGCCATAG TCGATATTGG AGATATTGAT 
GGGAACAAAT TCAGAGACGT GGCAGTGGGA GCTCCTCTGG AACCAGCAGA TGAGCTGACT 
GATGTTTCCG GAGCCATTTA CATCTATAAC GGATTTGAAT ATGGGATAAA GCAGCAGTTT 
TCACAGAGAA TTGCAGCTTC TGGTTTTGAG ATGAAACTTG TACATTTTGG TCAGGCAGTG 
AGTGCCATGA TGAAGAAAGA AGATTCAGAA AGAGACAAGA ACAATCTTTT ACTCAGTGTT 
GGGAGTCAAG GGGCTGTCAC TGTATTAAAG ACAGTTCCTG TCATCATTAT CACACCAAAA 
ATAACACTTG GTCTAACAGA GATTTCCCCT GATAAGCAGA ACAATGAAAA ATCAAGCCAA 
ATCGAGTCAT TTATCCAAAT CTGCTTTGAT AACACAAGGA AAAGAATTGA CAATGATGAA 
GAACTACTCA TTGAGTATCA AATTGATTTG GACTTCGGTA AAGAAGAAAA ACGACTAATT 
TTTGTTGAAC ACCATGAAAA GCAAACCAGA TTCACTCTGA CAAAATATAA AAACTGCATA 
AGCAAAATAA ATGTTAAATA TATGGGCTGC TATGACTGTT TTTCACCTAT AAAAATAAAG 
GTACAATTTG AATTGCCCTC ACACACTGAG GGAATCCCTG TGCGAGTGCT TGATGCTTTT 
AGTCCAACGG AGTTCACAAA AGAGATTGAG TTCCAAAAGG AGTGTAATAT CACAACAAGC 
TGCAAGCCAA AATTTTCATT AGATGGTTCA AAACTAAGTT ACAGCAATTA CTTGATCATA 
ATTGGATCCT CTCAATCCTT GGGTATAATC TTTGATCTTC AAAACTCTGG GAAAAGCTCG 
TATATCACAA CTCTGACTCT GACTTATCCT AACATACTAC AGGCAAAGAA AATTGAGGGC 
GGATCATGTG AGATGAAAAA TGAGGACCTT CAAATAATAT GCAAACTCTT ACATCCTGTT 
TTTAAGGGAG GTGCACAGAC CAAGGTCGCA ATCGACTGGC AGCCGATTAA CAGCAGAAAG 
TCAGACCTGA CCACTGGGAC GATCAGAGCT GTTTTAACAG GAGGAAATGC TGGCACTAAG 
ACCCTGGCCT CTAAGGAGTA TGATTTTGTA GTGAGGAATG CCCTAAAACT TCAGCTCACT 
GGCACAGCTT CACAAAACAT TTTCGCGATT GAAGAAGGAA AAGAAAGCCC AAATAAGGAC 
CCAAAAGTGC TGCAGTTCAC TTTTAAGCTA CTGGGTGACA ACAAGTACAA GGCTGAAATA 
CATGTGATGA TAACAATAGA AAAGAAAACA CAAGACATAA AACTGATCAT CAAGCAAGTT 
CGACCTCAAG AGCATTGTAA AACACTGTCT GGAGAACAGA ACGAGAGCAA CCTTCGTGTA 
GAATGCATTC TGACTGAATT GCAAGACATT ACCATCAACA CTGATGTCAT TATTAATGAC 
ATTAAGATTA CTGAGAGGAT CACTGCCAAA GCAGAACTCA GCTTTAATGA GAGCATCTAC 
GAAGGACAAG ACATGAGGAA GATGGAAAAG GTGGAAGTGG TAGTTATCAA GCAGAAGATT 
GTGAAATCAA CTGCAGTAAT TGCAGGTGGC TCCGTTGGGG GGTTTCTGCT GTTAATCATC 
ATCATCATTG TTCTTATAAA GTGTGGCTTC TTCCGTCGAC GGCATCATAT GGAGCGTACA 
CAGAGCACAC ACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

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itgae cDNA ORF clone, Astyanax mexicanus(Mexican tetra)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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