tep1 cDNA ORF clone, Astyanax mexicanus(Mexican tetra)

Gene
Clones
gRNAs

Summary

Gene Symbol	tep1
Entrez Gene ID	103036747
Full Name	telomerase associated protein 1
Gene Type	protein-coding
Organism	Astyanax mexicanus(Mexican tetra)

mRNA and Protein(s)

mRNA	Protein	Name
XM_015604764.1	XP_015460250.1	telomerase protein component 1-like
XM_022671227.1	XP_022526948.1	telomerase protein component 1

Pathways from BioSystems
Gene Ontology

The following tep1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the tep1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OAs115165	XM_022671227.1 Latest version!	Astyanax mexicanus telomerase associated protein 1 (tep1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1301.30 $1859.00
OAs27950	XM_015604764.1 Latest version!	Astyanax mexicanus telomerase protein component 1-like (LOC103036747), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

tep1 ( XM_022671227.1 ) cDNA ORF clone, Astyanax mexicanus(Mexican tetra) -> XP_022526948.1 Astyanax mexicanus telomerase associated protein 1 (tep1), mRNA.

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CloneID	OAs115165
Clone ID Related Accession (Same CDS sequence)	XM_022671227.1
Accession Version	XM_022671227.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7590bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1506873600000
Organism	Astyanax mexicanus(Mexican tetra)
Product	telomerase protein component 1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_035897.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Astyanax mexicanus Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## ab initio :: 1% of CDS bases ##RefSeq-Attributes-END##

ATGGAGGCGG CGACCCTGAT CCCCTCCGAC CCCAAGGTCA ATCCGCTGAT CCACTCCGAC 
CCCAAGGTCA ATCCAGAGGG CTTTGGTTTT TGCAACAGCG GCCCAAATCT GCTGTTACAA 
AAAGGTATGG AGAACAGGAT CCTGGCCCAG GCCTCTTCAC TTCTGCCCCA CGCTTCTGCT 
TTAACTCCAT CCTGGGCGAC TCCTTCCCCC TCCATCTCCT CCTGTTCTGC TCTGCAGCCC 
TCCTGGCTCT CCTCCACCTC CACCACTCAT CCTCTTCTCT CCTCCTCCAT CTCCTCCTCC 
TCTCATCTCC TGTCCTCCAC TGTGTTCTCC TCCTCCTCTC TGCTCAGCAC TAAGAACACC 
CAGCTGAAGC AAGATCTCCT GTCCTCCTCT CTCTGCTCTC CTCCCTCCAC CCTCCCTCTG 
CTCCACAGCA CATCTCTCTC TGCTGCTGTA GCTGATCCTC CTCAGCCAGA GCAGCTGAGT 
GTGGCTTTAA AAAAGAGGAA AATCGAGCTG GAAAGTCTGA GTTCCAACGA TTACACCACA 
TCACAAATCG AGGAGCTGAA CGAGGATTTA GAGAATGAGG ACCTGGTAGA GATGGACTCT 
GGGCACACAG AGTTGCCTGT GCTGGACCTG AGACTGAGTG ATGAAGAGGA GAAGCAGACA 
GTGGATGTTG GGGAGGTGTT CACTGTGTCC AGCACTGACT CAGACCGTAC TGTAGAGTTA 
CTAAAGGAAA AGAAGTATAT TCTGCTGAAT GTGGTGTGCT GCTCCCTGGT GAATAAAAGC 
AGTGCTCCGA ACCAGAACGA CTGGGACAGC AAGAAAAATG TTTGGACACG GATAAAAGAC 
CTCGGCACAG AGATCACCCG CAGAGACCCT GAGTTCATCC TTAAGGTGGC CGTCTACACC 
AGGCAGGAGC TCAACATCCG CATCACCACC AACTTCCTGC TGGCCCTGGC GGCCTACCTG 
CCAGAGTCTA AACCCCACCT GAGGAGGTAC TTCTGTGCAG CGGTGCAGCT GCCCTCTGAT 
TGGCTGGAAG TGACCAGACT GTTCAGCACG TGTTTCAGCT CGTCTCTCCC AGCATGCCTG 
AAAAAAGCCC TGGTGGACAA ATTCAAGCAG TTCAGCGAGT ATCAGCTGGC CAAGTACAAC 
ACCCGCAAAC TCCGCGGCAA ACACAACAAG AAGAAGCGTG AAGTCAAGGT AAATCTCACT 
ACTGACAATG ATGCACTACT GATAATTGAT CGTCCATGTG TTGTGTTGGC ACTAATTATT 
GTGTGTGTGT GTGTTGTTTT GTGCAGGTAC CCGTCTGATC TGAGGGCGTT TGTGAGCAGT 
GGTTTAAGTG GTGTGTGGGA GAGCGAGCGA GCCGGTCAGC GTATGAAGCT GAAGCAGCCG 
GACACCTGGG AGACCAAACT CAGTCTGGAG GGAAACAACG CGGCTGTGTG GGAGAAACTC 
ATAGACAATA ACTCTCTACC CTTCATGGCC ATGCTGAGGA ACCTGAGGAA CATGATCACT 
CAAGGGATCA GCGACCAGCA CCATGAACGC ATACTGAAAC GACTGACCTC AAAGAATGCT 
GTGATCCAGA GCAGGCAGTT TCCTTTCCGC TTCCTGTCTG CGTATAAAGT GATTATGGAG 
CTTAACAAGT TCGTGAGTGG TCCTGCCCAG CAGGGGATCA GCAAGTGCAT CATTTTACGG 
AAGATTGTGA CGAAACTTCC GAAGTGCAGA CGCTACCGGC GGAATGACTG GAGCACAGCC 
AGCCGGAAGA AGCTGCTGCA GACTTTGGGG GTTCCGGTTA TCTATGGCAT GTGCAAGATG 
AAGCTCAAGC AGCAGAAGAA GTTCAGTGCA GCTACCAGAG TGAGGAATTA CACTCCGGAG 
CTGCTGGTGC GTTATCGTCA GGCTCTGGAG AAAGCTGTTC AGATCTCCTG CAGGTACAAC 
ATCCCTCCTC TGCCTGGACG CACCATCATC ATCTTCAGGA GCGACTGGAC GCCAGCCCCG 
AACTGGAAAG GAGCTGACGA CATCTGCGTG CCTTCAGACC CTTCAGAAGA CTCGGACAAC 
ACCAGCCAGT CAGCTTCACT TGAGGAGGCG GCGGTGCTGC TGGCCCTGAT GATCAGCCAC 
TGCTGTGAGC ACCCGATGTT TCTGATACAC GACTTCGTTA ACGTACATGA AGTCAAGCTC 
AAATCCGACG TTTTCCTGGA GAATGTGAAA CATGTGATGA AGGAGCTCTC GGTAAAATTT 
CTTAAATATC TCAGACATGC CTCTACACTA GGGACTGTGT GGTTTTGTCC ACACTGGTCT 
CTCTCTCTGC AGGTGGATAA TATAATCCTG CTGAAGGATG TGAGTGTGTA TTATTCAGTA 
GATCAGGCTG TGGAGAAGTA CAGGAAGGAG TCGCTCAGTG ATCCTCTACT GGTCAATATC 
ATGATGAGAT CCAGTATGAA TAATTGTCGT TCTGAGGGTG GAGACCCCAG AAGTGTGTAT 
CTGAGTGGGT TTAGTGAGCA GATCCTCAGG TTTGTTTCAG AAAGAGGCTC CTCCAGGTTT 
TTGGATCATG TAGAGAGCAT CGACAGGCTG CGCAACATCC CCCCACCAGC AGGTGGCTCC 
ACCAAACCTG AGAGAGCAGC CGACATCACC CCATTGCCCG TCACTCCTAA ACTCAGGTGG 
CAAGGGGTTC GTGTTTTTAT CTCCTCTACG TTCAGGGACA TGCATGCTGA GCGGGACGTC 
CTGGTGCGGA GCGTGTTTCC GGAGCTGCGG CGCCGTGCAG CTCCACACTG CCTCTACCTG 
CAGGAGGTGG AACTGCGCTG GGGCGTAACC GAGGAGGAGT CTAACCAGGC AATCCAGCTC 
TGTCTGTCCG AGGTGTGCCG CAGCCAGCTG CTGCTCAGCA TTCTGGGAGA GAGATACGGG 
CAGGTGCCAC CTCGACCTAC ACTTCCTGAG CTTCCTCAGT ACACCTGGCT GAACTCGGCC 
CCTGATGGGA TGTCGATCAC AGAGATGGAG ATTCGTCAGT TTCAGGCTGT GTATCCAGAC 
TCCGCCCAGG GCAGAATGTT CTTCTACTTC AGATCTCCTC ACCTGGCCAG TTCTGTTCCT 
GTGGAGTGGA GAGAGGACTT CTCGTCCGAG TCGAGCCAAT CAGCAGCTAA AATGAGCGAT 
CTGAAAGAAT GGATCCGGAG CAACCAGTTC AGAGTCACAG AGGATTACCC GTGTGAGTGG 
GGCGGGGCTG TCGACGGAAG GCCGTATGTG AAAGGTCTGG AGGATTTTGC GAAAGCAGCT 
CTGGAGGATC TGTGGGAAGC CCTGGAGAAA CTCTTTGTGG AGGAAGTAGA CGAGGCTGAT 
CTGATGTCGG AGATTAATGA ACAGGAGGTT CATCAGGACG CTCAGCAGCG GCATTTCTAC 
GGCCGGAAGA AACTGGTTTC CTCAGCAATA GAGAAAATTC AGGAGTGCCA GCAGAAAGGA 
GGCATCCTGC TGGTGGAGGG AGCTCAGGGA GAGGGCAAGA CCACCTTTAT GGCTGGTCTG 
GCTCACGCGC TCCAAACTCC AGATAAGCGC AGAAAAGTGC CAGTTTGTGA TGTCATATCC 
TATTCCACAG AGGCTAGCCA ATCAGCTTCT GGCGTAGAGC ACCTCCTACG CTGCCTCATC 
CAATGGCTGA GGAGGAGGAA ACAACAGGAG GAGGAGTTAG TCACTAGCAC ACCTTACAGG 
GGTGTCTGTC TGGTTCTGAG TGTCACCAGA GGCTCCGCCC TCCGCACCAC CCTCTCAGGT 
AAGCAGGGGT GTGTGGTGTT CGGGATGGGT CAGTTATCTC TGCCGGACAG GAAGGAGATT 
GTGCAGAAGG AGCTGAGTGT GTACGGGAAG AAGCTGAGTG ACTCTGCCTT TAATAACCAG 
CTTCAGACGC TGCTGATGAA GAAAGGAGCC GTCAGCCCAC TCTACCTCCA CTTCACCTGC 
GAGGAGCTGA GGAACTACGC CTCCTTCGAA ACGATGAAGA GGAACCTGCA GACTCTTCCT 
CCGTCTCTGT GTGAGCTGGT GCAGCACAGT CTGCTCAGGC TGCAGTCTCA ATACGGGGCG 
GCGGGGCTGG ACTGGACCCT GGCGGCGCTG ACCGTCAGCT CTACAGGTCT GAGGGAGAGA 
GATCTGTACG CTGTGCTGAA CCTCTGCAGT GACCTGATCT CCACACAGGG TCAGCTCACC 
TGGAACAGAG CGCTCACAGC GGCCCGGAGC CCGAGGAGCC GTGTGCCCAT GGCGCTGTTC 
TCTCAGCTGG CACAGGGTTT ACGGGGTTTG ATCCGTCCGT CTCTCTCTCA TGAGCCCGAC 
GAGCCCCTGA CCCTGACCCA CCCGGATGTG AAGCTGGCGT TTGAGCAGCT GTATCTCTCC 
ACTGATCAGG ACAGAGCCAG AGCACACCTA TTACTGGCAG CTCACCTGTG GGTGCGGTCG 
GACCCTCTAG GTAACGACAC ATTTCTTCAC TGTGAGGCAG ATGCTCTGCT GCATCTTCCT 
GCTCATCTGA TGGAGAGTTC TCAGTGGCAG CCCCTGCTCC TACTGCTCTC TAGTTACTAC 
TTCCTGTTCG CCAACGTGCT CCATGGACTC CTGCACTCTC TACTGGAGAC GTACATCCTG 
TTTGCAAAGA GAACTGAAGC TGAGCAGCAG AGCTGGACTG AAACATCAGG TGATCTAAAG 
GAGCTTCAGG CCTGTCACTC ATTTCTGAAG CGCCACGCCC CTCTCCTGTC CTGCTGGCCG 
CGGCTGTTTG TGCAGCAGGC TCTGAACGAA GCGGCCGGCT CGTCTGCTCA CGCCTGGGCT 
CGCACGCTGG TGGAGGAGGG AGGAGTGAAC GCAGTCAGGA ACCTGAACAA CACTGACGGC 
CCTCAGCTGG AAACTGGAGA GCTGGTGTCC ACCTTCCAGG CCGAGCCGTC CTGTGTGGCT 
CTGAGTCCGG GGGGAGGGTG TGTGGCTGTG GGGACGGAGC AGGGAACCAT ACACTTCATC 
CACACACACA CCAACCAGGA AGTGAAGTCA CTGGTCAGTA ACTGTGATGG CATCTCCAGC 
TGTGTGTTTC TGGATGACGG ATTTCTAAGC ACCACATCAT ATGATGGGCA GGTGGAGGTG 
TGGGATATTA ACAGTGGCAG CAGGACTGTC CACCTGAACG CCCACTCCAA CAGGATCACA 
GGATGTGATG TCAGCTCAGA TAAGAAGCAC TTTGCCACAG TCTCTTTAGA CTCCAATCTG 
AAGGTGTGGT CGTCTCAGAA GGGAAAGCAG GTGGTGTCTC TGCTCACCCC CTGCCCTCTA 
AACTGTGTGA CCTTTGACCC TGAGGGAGGT CTGCTGGCGG TGGGCTGCTG GGACAGAACG 
GTGCGAGTGT GGAACTGGAT CAAACAGGAA AGATGGACGA CTCTCTCAGG TCATCAGTCG 
TCGGTGCGCA GTGTCTCGTT CTCTCCCTCC TCCTCTCTGC TGTGCTCTGG TAGTCTGGAT 
GGTGAGGTGC TGCTGTGGTC ATCCTCTGGC TTGGCCTGTG TGGGGCGCTA TCAGGCTCAC 
CGTGGCTCTA CTGAGGCCCT GAGTTTCCTG CCTGGAGGAG AAACACTGCT GAGTGCTGGA 
CACGATGGCA TGGTTCAGTT GTGGTCTGGA GGTCTGGGGT GCTCTGTTTC TGTGCTGGGA 
GAGGAAACGA AAGCGGTGAA GTCGAGTGTG TCCAGAGCTG CAGCAGTCTC TGAAAGTGCC 
GCTCTCTCTG TGGCTGGAGC TCATGGATAC GCTGCTGTGG GATATCATGG AGATGGTATC 
AGACTGTTTG AGCTGAAATC AGGTGAGAGG GTGTGGTCCA CTGAGGACCT GCGGGTGTCT 
GTGCCGTGCC TGCTGTGGAT GGAGGCTGCT GGGGTGGATC TGCTTTTGTC TGGAGCTTTA 
GACAGTGGTC TCCGGCTGTG GAGCAGGCAG GGGGCTGGTA TGACCCTCAG GGGGGCTTTT 
GGGGTACAGA AGGGGGGGAT CCTCTCATTG GCTCAGAGCT CTGTGTACGT GGCGTCTGCT 
TCAGACGACT TCACCATCGC TCTGTGGCTG AAGAGTGACC TGACCTCTGA CCCCTGGGTT 
CAGCCCAGTG CGGTTAGTGT ACTGAGGGGT CACAGTGGAG GGGTCACGTG TCTGGCCTTC 
AGTCCAGACG GAGACCATTT ACTGTCTGGA GGCAAAGACA AGGCTTTGAT GGTGTGGAAG 
GTGGACTCCT CTCCTCCGGT TCTCTCTCGC TCTCTTCCTC ACTGCCACGG AGACTGGATC 
ACAGACTGTG CGTGGACACC AGCAGCTCTG CTGAGCTGCT CGAGTGACTG TAGGCTGAGA 
CTGTGGGACG TGCAGACGGG ACGCTGCCTC CGAGAAGTTT CAGCTGCTTC ATCTCTCAGC 
GCTCTGAGCT GCTGGGAGGA TTATGTAATG CTGGGCTGTG CTGATGGTCT GCTGATGGTG 
TGGAAATCAG ATATTGGAGT TATCGCTGAG ATCCATGCTC ACCAGTCCCG TGTCCACCAC 
AGCACCATTA CCACTGCACC TGTAGGTGGG CAGAAGGTGG AGGAGCTGGT GGTGGCCACA 
GCGTCTGATG ATGGAACTGT GAAGGTCTGG CTCCCTCTGC AGGTTCAGCA CCGCAGCACT 
CTGCTGGGTC ACAGCGGTGC TGTCCAGGCG CTGGCGAGTG GACGAGGTCC ACAGTTCCTC 
ACGGTGTCTG AGGACCGCTC TCTCCGAGCC TGGAGCGTTC GTACAGAGAA TCCTCCCTTT 
CAGGAAGAGT GTGTGAGTGC AGTGTGTTTT CTGGATTCAG AGAAGCTGCT GGTGTGTGGT 
TATACCTCAG GAAAACTGGA GATATGGCAC AACACTTTAA AGATCTACTC CAGTAAGGTG 
AGCGACAGTC GTATTCGAGC GGTGAGCGGA ATGCCGGATC AGAGCCTGGC TGTGGGCTGC 
AGTGATTACA CTGTGACTGT CTTTAAACTG ACCAGAGACC ATCAGCACTG CACTGCCAGT 
TTGACTAAAG GATCGTTGTA CACTTTGGAG AAGGGTGTGA ATTTCCTGTG TTACTGTAAC 
ACTCTGATGG GAGTGTGTGT GGACAGTTCT GTCATCGACA TCTTCTCCAC TGTGACCGAC 
AATACAAGAA TGATCGACAC GTGGCAGTAC ACCATGCGGA CTATGGGCGT TTTCAAGAGT 
GATGTAAACA GCCTGTGGCT GCTGGGAGAG GAAAACGACA ACATCCACCT TTCATACCTG 
CTCTGTGCCA GTGCGGAACT GACCTCAAAT TCAACGACTG TGAGTGCGAA AGTGGGAGGG 
GACAAGGACA GTGATGATGA AGATGAGAAG GACACGGAGA AAGAGCAGAC TTTCACCATT 
ACAGCTGCTA CCATGCACAA CAATTTTATC GTGTGTGGAG ACTCTAAAGG CAATATGTGG 
TTCAACAATG TGTCGAACAC GGACAGCTGG AGCCCAAGAA GACCCGTTCA CAGTGACAGG 
ATCAGTGTTC TCCGGCTGAC GGACAGCACT ATAATCTCCG CCTCTCACGA CAGAACCGTC 
AAACTGTGGG ACAGAACCAC CAACAAACAG GTGGGTCTGT TTGTGTGTGC TGCTGCGGTG 
GAGGTGATGG AGGTGGACCC CGCGGACCCC TGCAGGCTGG TGTGTGGAGA TGCGCTGGGG 
AAGCTCTACT TCCTGTCCTG GAAAGAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_022526948.1
CDS	203..7792
Translation	MEAATLIPSDPKVNPLIHSDPKVNPEGFGFCNSGPNLLLQKGMENRILAQASSLLPHASALTPSWATPSPSISSCSALQPSWLSSTSTTHPLLSSSISSSSHLLSSTVFSSSSLLSTKNTQLKQDLLSSSLCSPPSTLPLLHSTSLSAAVADPPQPEQLSVALKKRKIELESLSSNDYTTSQIEELNEDLENEDLVEMDSGHTELPVLDLRLSDEEEKQTVDVGEVFTVSSTDSDRTVELLKEKKYILLNVVCCSLVNKSSAPNQNDWDSKKNVWTRIKDLGTEITRRDPEFILKVAVYTRQELNIRITTNFLLALAAYLPESKPHLRRYFCAAVQLPSDWLEVTRLFSTCFSSSLPACLKKALVDKFKQFSEYQLAKYNTRKLRGKHNKKKREVKVNLTTDNDALLIIDRPCVVLALIIVCVCVVLCRYPSDLRAFVSSGLSGVWESERAGQRMKLKQPDTWETKLSLEGNNAAVWEKLIDNNSLPFMAMLRNLRNMITQGISDQHHERILKRLTSKNAVIQSRQFPFRFLSAYKVIMELNKFVSGPAQQGISKCIILRKIVTKLPKCRRYRRNDWSTASRKKLLQTLGVPVIYGMCKMKLKQQKKFSAATRVRNYTPELLVRYRQALEKAVQISCRYNIPPLPGRTIIIFRSDWTPAPNWKGADDICVPSDPSEDSDNTSQSASLEEAAVLLALMISHCCEHPMFLIHDFVNVHEVKLKSDVFLENVKHVMKELSVKFLKYLRHASTLGTVWFCPHWSLSLQVDNIILLKDVSVYYSVDQAVEKYRKESLSDPLLVNIMMRSSMNNCRSEGGDPRSVYLSGFSEQILRFVSERGSSRFLDHVESIDRLRNIPPPAGGSTKPERAADITPLPVTPKLRWQGVRVFISSTFRDMHAERDVLVRSVFPELRRRAAPHCLYLQEVELRWGVTEEESNQAIQLCLSEVCRSQLLLSILGERYGQVPPRPTLPELPQYTWLNSAPDGMSITEMEIRQFQAVYPDSAQGRMFFYFRSPHLASSVPVEWREDFSSESSQSAAKMSDLKEWIRSNQFRVTEDYPCEWGGAVDGRPYVKGLEDFAKAALEDLWEALEKLFVEEVDEADLMSEINEQEVHQDAQQRHFYGRKKLVSSAIEKIQECQQKGGILLVEGAQGEGKTTFMAGLAHALQTPDKRRKVPVCDVISYSTEASQSASGVEHLLRCLIQWLRRRKQQEEELVTSTPYRGVCLVLSVTRGSALRTTLSGKQGCVVFGMGQLSLPDRKEIVQKELSVYGKKLSDSAFNNQLQTLLMKKGAVSPLYLHFTCEELRNYASFETMKRNLQTLPPSLCELVQHSLLRLQSQYGAAGLDWTLAALTVSSTGLRERDLYAVLNLCSDLISTQGQLTWNRALTAARSPRSRVPMALFSQLAQGLRGLIRPSLSHEPDEPLTLTHPDVKLAFEQLYLSTDQDRARAHLLLAAHLWVRSDPLGNDTFLHCEADALLHLPAHLMESSQWQPLLLLLSSYYFLFANVLHGLLHSLLETYILFAKRTEAEQQSWTETSGDLKELQACHSFLKRHAPLLSCWPRLFVQQALNEAAGSSAHAWARTLVEEGGVNAVRNLNNTDGPQLETGELVSTFQAEPSCVALSPGGGCVAVGTEQGTIHFIHTHTNQEVKSLVSNCDGISSCVFLDDGFLSTTSYDGQVEVWDINSGSRTVHLNAHSNRITGCDVSSDKKHFATVSLDSNLKVWSSQKGKQVVSLLTPCPLNCVTFDPEGGLLAVGCWDRTVRVWNWIKQERWTTLSGHQSSVRSVSFSPSSSLLCSGSLDGEVLLWSSSGLACVGRYQAHRGSTEALSFLPGGETLLSAGHDGMVQLWSGGLGCSVSVLGEETKAVKSSVSRAAAVSESAALSVAGAHGYAAVGYHGDGIRLFELKSGERVWSTEDLRVSVPCLLWMEAAGVDLLLSGALDSGLRLWSRQGAGMTLRGAFGVQKGGILSLAQSSVYVASASDDFTIALWLKSDLTSDPWVQPSAVSVLRGHSGGVTCLAFSPDGDHLLSGGKDKALMVWKVDSSPPVLSRSLPHCHGDWITDCAWTPAALLSCSSDCRLRLWDVQTGRCLREVSAASSLSALSCWEDYVMLGCADGLLMVWKSDIGVIAEIHAHQSRVHHSTITTAPVGGQKVEELVVATASDDGTVKVWLPLQVQHRSTLLGHSGAVQALASGRGPQFLTVSEDRSLRAWSVRTENPPFQEECVSAVCFLDSEKLLVCGYTSGKLEIWHNTLKIYSSKVSDSRIRAVSGMPDQSLAVGCSDYTVTVFKLTRDHQHCTASLTKGSLYTLEKGVNFLCYCNTLMGVCVDSSVIDIFSTVTDNTRMIDTWQYTMRTMGVFKSDVNSLWLLGEENDNIHLSYLLCASAELTSNSTTVSAKVGGDKDSDDEDEKDTEKEQTFTITAATMHNNFIVCGDSKGNMWFNNVSNTDSWSPRRPVHSDRISVLRLTDSTIISASHDRTVKLWDRTTNKQVGLFVCAAAVEVMEVDPADPCRLVCGDALGKLYFLSWKE

Target ORF information:

RefSeq Version	XM_022671227.1
Organism	Astyanax mexicanus(Mexican tetra)
Definition	Astyanax mexicanus telomerase associated protein 1 (tep1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_022671227.1

ORF Insert Sequence:

ATGGAGGCGG CGACCCTGAT CCCCTCCGAC CCCAAGGTCA ATCCGCTGAT CCACTCCGAC 
CCCAAGGTCA ATCCAGAGGG CTTTGGTTTT TGCAACAGCG GCCCAAATCT GCTGTTACAA 
AAAGGTATGG AGAACAGGAT CCTGGCCCAG GCCTCTTCAC TTCTGCCCCA CGCTTCTGCT 
TTAACTCCAT CCTGGGCGAC TCCTTCCCCC TCCATCTCCT CCTGTTCTGC TCTGCAGCCC 
TCCTGGCTCT CCTCCACCTC CACCACTCAT CCTCTTCTCT CCTCCTCCAT CTCCTCCTCC 
TCTCATCTCC TGTCCTCCAC TGTGTTCTCC TCCTCCTCTC TGCTCAGCAC TAAGAACACC 
CAGCTGAAGC AAGATCTCCT GTCCTCCTCT CTCTGCTCTC CTCCCTCCAC CCTCCCTCTG 
CTCCACAGCA CATCTCTCTC TGCTGCTGTA GCTGATCCTC CTCAGCCAGA GCAGCTGAGT 
GTGGCTTTAA AAAAGAGGAA AATCGAGCTG GAAAGTCTGA GTTCCAACGA TTACACCACA 
TCACAAATCG AGGAGCTGAA CGAGGATTTA GAGAATGAGG ACCTGGTAGA GATGGACTCT 
GGGCACACAG AGTTGCCTGT GCTGGACCTG AGACTGAGTG ATGAAGAGGA GAAGCAGACA 
GTGGATGTTG GGGAGGTGTT CACTGTGTCC AGCACTGACT CAGACCGTAC TGTAGAGTTA 
CTAAAGGAAA AGAAGTATAT TCTGCTGAAT GTGGTGTGCT GCTCCCTGGT GAATAAAAGC 
AGTGCTCCGA ACCAGAACGA CTGGGACAGC AAGAAAAATG TTTGGACACG GATAAAAGAC 
CTCGGCACAG AGATCACCCG CAGAGACCCT GAGTTCATCC TTAAGGTGGC CGTCTACACC 
AGGCAGGAGC TCAACATCCG CATCACCACC AACTTCCTGC TGGCCCTGGC GGCCTACCTG 
CCAGAGTCTA AACCCCACCT GAGGAGGTAC TTCTGTGCAG CGGTGCAGCT GCCCTCTGAT 
TGGCTGGAAG TGACCAGACT GTTCAGCACG TGTTTCAGCT CGTCTCTCCC AGCATGCCTG 
AAAAAAGCCC TGGTGGACAA ATTCAAGCAG TTCAGCGAGT ATCAGCTGGC CAAGTACAAC 
ACCCGCAAAC TCCGCGGCAA ACACAACAAG AAGAAGCGTG AAGTCAAGGT AAATCTCACT 
ACTGACAATG ATGCACTACT GATAATTGAT CGTCCATGTG TTGTGTTGGC ACTAATTATT 
GTGTGTGTGT GTGTTGTTTT GTGCAGGTAC CCGTCTGATC TGAGGGCGTT TGTGAGCAGT 
GGTTTAAGTG GTGTGTGGGA GAGCGAGCGA GCCGGTCAGC GTATGAAGCT GAAGCAGCCG 
GACACCTGGG AGACCAAACT CAGTCTGGAG GGAAACAACG CGGCTGTGTG GGAGAAACTC 
ATAGACAATA ACTCTCTACC CTTCATGGCC ATGCTGAGGA ACCTGAGGAA CATGATCACT 
CAAGGGATCA GCGACCAGCA CCATGAACGC ATACTGAAAC GACTGACCTC AAAGAATGCT 
GTGATCCAGA GCAGGCAGTT TCCTTTCCGC TTCCTGTCTG CGTATAAAGT GATTATGGAG 
CTTAACAAGT TCGTGAGTGG TCCTGCCCAG CAGGGGATCA GCAAGTGCAT CATTTTACGG 
AAGATTGTGA CGAAACTTCC GAAGTGCAGA CGCTACCGGC GGAATGACTG GAGCACAGCC 
AGCCGGAAGA AGCTGCTGCA GACTTTGGGG GTTCCGGTTA TCTATGGCAT GTGCAAGATG 
AAGCTCAAGC AGCAGAAGAA GTTCAGTGCA GCTACCAGAG TGAGGAATTA CACTCCGGAG 
CTGCTGGTGC GTTATCGTCA GGCTCTGGAG AAAGCTGTTC AGATCTCCTG CAGGTACAAC 
ATCCCTCCTC TGCCTGGACG CACCATCATC ATCTTCAGGA GCGACTGGAC GCCAGCCCCG 
AACTGGAAAG GAGCTGACGA CATCTGCGTG CCTTCAGACC CTTCAGAAGA CTCGGACAAC 
ACCAGCCAGT CAGCTTCACT TGAGGAGGCG GCGGTGCTGC TGGCCCTGAT GATCAGCCAC 
TGCTGTGAGC ACCCGATGTT TCTGATACAC GACTTCGTTA ACGTACATGA AGTCAAGCTC 
AAATCCGACG TTTTCCTGGA GAATGTGAAA CATGTGATGA AGGAGCTCTC GGTAAAATTT 
CTTAAATATC TCAGACATGC CTCTACACTA GGGACTGTGT GGTTTTGTCC ACACTGGTCT 
CTCTCTCTGC AGGTGGATAA TATAATCCTG CTGAAGGATG TGAGTGTGTA TTATTCAGTA 
GATCAGGCTG TGGAGAAGTA CAGGAAGGAG TCGCTCAGTG ATCCTCTACT GGTCAATATC 
ATGATGAGAT CCAGTATGAA TAATTGTCGT TCTGAGGGTG GAGACCCCAG AAGTGTGTAT 
CTGAGTGGGT TTAGTGAGCA GATCCTCAGG TTTGTTTCAG AAAGAGGCTC CTCCAGGTTT 
TTGGATCATG TAGAGAGCAT CGACAGGCTG CGCAACATCC CCCCACCAGC AGGTGGCTCC 
ACCAAACCTG AGAGAGCAGC CGACATCACC CCATTGCCCG TCACTCCTAA ACTCAGGTGG 
CAAGGGGTTC GTGTTTTTAT CTCCTCTACG TTCAGGGACA TGCATGCTGA GCGGGACGTC 
CTGGTGCGGA GCGTGTTTCC GGAGCTGCGG CGCCGTGCAG CTCCACACTG CCTCTACCTG 
CAGGAGGTGG AACTGCGCTG GGGCGTAACC GAGGAGGAGT CTAACCAGGC AATCCAGCTC 
TGTCTGTCCG AGGTGTGCCG CAGCCAGCTG CTGCTCAGCA TTCTGGGAGA GAGATACGGG 
CAGGTGCCAC CTCGACCTAC ACTTCCTGAG CTTCCTCAGT ACACCTGGCT GAACTCGGCC 
CCTGATGGGA TGTCGATCAC AGAGATGGAG ATTCGTCAGT TTCAGGCTGT GTATCCAGAC 
TCCGCCCAGG GCAGAATGTT CTTCTACTTC AGATCTCCTC ACCTGGCCAG TTCTGTTCCT 
GTGGAGTGGA GAGAGGACTT CTCGTCCGAG TCGAGCCAAT CAGCAGCTAA AATGAGCGAT 
CTGAAAGAAT GGATCCGGAG CAACCAGTTC AGAGTCACAG AGGATTACCC GTGTGAGTGG 
GGCGGGGCTG TCGACGGAAG GCCGTATGTG AAAGGTCTGG AGGATTTTGC GAAAGCAGCT 
CTGGAGGATC TGTGGGAAGC CCTGGAGAAA CTCTTTGTGG AGGAAGTAGA CGAGGCTGAT 
CTGATGTCGG AGATTAATGA ACAGGAGGTT CATCAGGACG CTCAGCAGCG GCATTTCTAC 
GGCCGGAAGA AACTGGTTTC CTCAGCAATA GAGAAAATTC AGGAGTGCCA GCAGAAAGGA 
GGCATCCTGC TGGTGGAGGG AGCTCAGGGA GAGGGCAAGA CCACCTTTAT GGCTGGTCTG 
GCTCACGCGC TCCAAACTCC AGATAAGCGC AGAAAAGTGC CAGTTTGTGA TGTCATATCC 
TATTCCACAG AGGCTAGCCA ATCAGCTTCT GGCGTAGAGC ACCTCCTACG CTGCCTCATC 
CAATGGCTGA GGAGGAGGAA ACAACAGGAG GAGGAGTTAG TCACTAGCAC ACCTTACAGG 
GGTGTCTGTC TGGTTCTGAG TGTCACCAGA GGCTCCGCCC TCCGCACCAC CCTCTCAGGT 
AAGCAGGGGT GTGTGGTGTT CGGGATGGGT CAGTTATCTC TGCCGGACAG GAAGGAGATT 
GTGCAGAAGG AGCTGAGTGT GTACGGGAAG AAGCTGAGTG ACTCTGCCTT TAATAACCAG 
CTTCAGACGC TGCTGATGAA GAAAGGAGCC GTCAGCCCAC TCTACCTCCA CTTCACCTGC 
GAGGAGCTGA GGAACTACGC CTCCTTCGAA ACGATGAAGA GGAACCTGCA GACTCTTCCT 
CCGTCTCTGT GTGAGCTGGT GCAGCACAGT CTGCTCAGGC TGCAGTCTCA ATACGGGGCG 
GCGGGGCTGG ACTGGACCCT GGCGGCGCTG ACCGTCAGCT CTACAGGTCT GAGGGAGAGA 
GATCTGTACG CTGTGCTGAA CCTCTGCAGT GACCTGATCT CCACACAGGG TCAGCTCACC 
TGGAACAGAG CGCTCACAGC GGCCCGGAGC CCGAGGAGCC GTGTGCCCAT GGCGCTGTTC 
TCTCAGCTGG CACAGGGTTT ACGGGGTTTG ATCCGTCCGT CTCTCTCTCA TGAGCCCGAC 
GAGCCCCTGA CCCTGACCCA CCCGGATGTG AAGCTGGCGT TTGAGCAGCT GTATCTCTCC 
ACTGATCAGG ACAGAGCCAG AGCACACCTA TTACTGGCAG CTCACCTGTG GGTGCGGTCG 
GACCCTCTAG GTAACGACAC ATTTCTTCAC TGTGAGGCAG ATGCTCTGCT GCATCTTCCT 
GCTCATCTGA TGGAGAGTTC TCAGTGGCAG CCCCTGCTCC TACTGCTCTC TAGTTACTAC 
TTCCTGTTCG CCAACGTGCT CCATGGACTC CTGCACTCTC TACTGGAGAC GTACATCCTG 
TTTGCAAAGA GAACTGAAGC TGAGCAGCAG AGCTGGACTG AAACATCAGG TGATCTAAAG 
GAGCTTCAGG CCTGTCACTC ATTTCTGAAG CGCCACGCCC CTCTCCTGTC CTGCTGGCCG 
CGGCTGTTTG TGCAGCAGGC TCTGAACGAA GCGGCCGGCT CGTCTGCTCA CGCCTGGGCT 
CGCACGCTGG TGGAGGAGGG AGGAGTGAAC GCAGTCAGGA ACCTGAACAA CACTGACGGC 
CCTCAGCTGG AAACTGGAGA GCTGGTGTCC ACCTTCCAGG CCGAGCCGTC CTGTGTGGCT 
CTGAGTCCGG GGGGAGGGTG TGTGGCTGTG GGGACGGAGC AGGGAACCAT ACACTTCATC 
CACACACACA CCAACCAGGA AGTGAAGTCA CTGGTCAGTA ACTGTGATGG CATCTCCAGC 
TGTGTGTTTC TGGATGACGG ATTTCTAAGC ACCACATCAT ATGATGGGCA GGTGGAGGTG 
TGGGATATTA ACAGTGGCAG CAGGACTGTC CACCTGAACG CCCACTCCAA CAGGATCACA 
GGATGTGATG TCAGCTCAGA TAAGAAGCAC TTTGCCACAG TCTCTTTAGA CTCCAATCTG 
AAGGTGTGGT CGTCTCAGAA GGGAAAGCAG GTGGTGTCTC TGCTCACCCC CTGCCCTCTA 
AACTGTGTGA CCTTTGACCC TGAGGGAGGT CTGCTGGCGG TGGGCTGCTG GGACAGAACG 
GTGCGAGTGT GGAACTGGAT CAAACAGGAA AGATGGACGA CTCTCTCAGG TCATCAGTCG 
TCGGTGCGCA GTGTCTCGTT CTCTCCCTCC TCCTCTCTGC TGTGCTCTGG TAGTCTGGAT 
GGTGAGGTGC TGCTGTGGTC ATCCTCTGGC TTGGCCTGTG TGGGGCGCTA TCAGGCTCAC 
CGTGGCTCTA CTGAGGCCCT GAGTTTCCTG CCTGGAGGAG AAACACTGCT GAGTGCTGGA 
CACGATGGCA TGGTTCAGTT GTGGTCTGGA GGTCTGGGGT GCTCTGTTTC TGTGCTGGGA 
GAGGAAACGA AAGCGGTGAA GTCGAGTGTG TCCAGAGCTG CAGCAGTCTC TGAAAGTGCC 
GCTCTCTCTG TGGCTGGAGC TCATGGATAC GCTGCTGTGG GATATCATGG AGATGGTATC 
AGACTGTTTG AGCTGAAATC AGGTGAGAGG GTGTGGTCCA CTGAGGACCT GCGGGTGTCT 
GTGCCGTGCC TGCTGTGGAT GGAGGCTGCT GGGGTGGATC TGCTTTTGTC TGGAGCTTTA 
GACAGTGGTC TCCGGCTGTG GAGCAGGCAG GGGGCTGGTA TGACCCTCAG GGGGGCTTTT 
GGGGTACAGA AGGGGGGGAT CCTCTCATTG GCTCAGAGCT CTGTGTACGT GGCGTCTGCT 
TCAGACGACT TCACCATCGC TCTGTGGCTG AAGAGTGACC TGACCTCTGA CCCCTGGGTT 
CAGCCCAGTG CGGTTAGTGT ACTGAGGGGT CACAGTGGAG GGGTCACGTG TCTGGCCTTC 
AGTCCAGACG GAGACCATTT ACTGTCTGGA GGCAAAGACA AGGCTTTGAT GGTGTGGAAG 
GTGGACTCCT CTCCTCCGGT TCTCTCTCGC TCTCTTCCTC ACTGCCACGG AGACTGGATC 
ACAGACTGTG CGTGGACACC AGCAGCTCTG CTGAGCTGCT CGAGTGACTG TAGGCTGAGA 
CTGTGGGACG TGCAGACGGG ACGCTGCCTC CGAGAAGTTT CAGCTGCTTC ATCTCTCAGC 
GCTCTGAGCT GCTGGGAGGA TTATGTAATG CTGGGCTGTG CTGATGGTCT GCTGATGGTG 
TGGAAATCAG ATATTGGAGT TATCGCTGAG ATCCATGCTC ACCAGTCCCG TGTCCACCAC 
AGCACCATTA CCACTGCACC TGTAGGTGGG CAGAAGGTGG AGGAGCTGGT GGTGGCCACA 
GCGTCTGATG ATGGAACTGT GAAGGTCTGG CTCCCTCTGC AGGTTCAGCA CCGCAGCACT 
CTGCTGGGTC ACAGCGGTGC TGTCCAGGCG CTGGCGAGTG GACGAGGTCC ACAGTTCCTC 
ACGGTGTCTG AGGACCGCTC TCTCCGAGCC TGGAGCGTTC GTACAGAGAA TCCTCCCTTT 
CAGGAAGAGT GTGTGAGTGC AGTGTGTTTT CTGGATTCAG AGAAGCTGCT GGTGTGTGGT 
TATACCTCAG GAAAACTGGA GATATGGCAC AACACTTTAA AGATCTACTC CAGTAAGGTG 
AGCGACAGTC GTATTCGAGC GGTGAGCGGA ATGCCGGATC AGAGCCTGGC TGTGGGCTGC 
AGTGATTACA CTGTGACTGT CTTTAAACTG ACCAGAGACC ATCAGCACTG CACTGCCAGT 
TTGACTAAAG GATCGTTGTA CACTTTGGAG AAGGGTGTGA ATTTCCTGTG TTACTGTAAC 
ACTCTGATGG GAGTGTGTGT GGACAGTTCT GTCATCGACA TCTTCTCCAC TGTGACCGAC 
AATACAAGAA TGATCGACAC GTGGCAGTAC ACCATGCGGA CTATGGGCGT TTTCAAGAGT 
GATGTAAACA GCCTGTGGCT GCTGGGAGAG GAAAACGACA ACATCCACCT TTCATACCTG 
CTCTGTGCCA GTGCGGAACT GACCTCAAAT TCAACGACTG TGAGTGCGAA AGTGGGAGGG 
GACAAGGACA GTGATGATGA AGATGAGAAG GACACGGAGA AAGAGCAGAC TTTCACCATT 
ACAGCTGCTA CCATGCACAA CAATTTTATC GTGTGTGGAG ACTCTAAAGG CAATATGTGG 
TTCAACAATG TGTCGAACAC GGACAGCTGG AGCCCAAGAA GACCCGTTCA CAGTGACAGG 
ATCAGTGTTC TCCGGCTGAC GGACAGCACT ATAATCTCCG CCTCTCACGA CAGAACCGTC 
AAACTGTGGG ACAGAACCAC CAACAAACAG GTGGGTCTGT TTGTGTGTGC TGCTGCGGTG 
GAGGTGATGG AGGTGGACCC CGCGGACCCC TGCAGGCTGG TGTGTGGAGA TGCGCTGGGG 
AAGCTCTACT TCCTGTCCTG GAAAGAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

tep1 ( XM_015604764.1 ) cDNA ORF clone, Astyanax mexicanus(Mexican tetra) -> XP_015460250.1 Astyanax mexicanus telomerase protein component 1-like (LOC103036747), mRNA.

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CloneID	OAs27950
Clone ID Related Accession (Same CDS sequence)	XM_015604764.1
Accession Version	XM_015604764.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6699bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1455033600000
Organism	Astyanax mexicanus(Mexican tetra)
Product	telomerase protein component 1-like
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_006749275.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Astyanax mexicanus Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## ab initio :: 1% of CDS bases ##RefSeq-Attributes-END##

ATGGAGGCGG CGACCCTGAT CCCCTCCGAC CCCAAGGTCA ATCCGCTGAT CCACTCCGAC 
CCCAAGGTCA ATTCAGAGGC CTTTAATTAT TGCAACAGTG GCCCAAATCT GCTGTTACAA 
AAAGGTATGG AGAACAGGAT CCTGGCCCAG GCCTCTTCTC TTCTGCCCCA CGCTCCTGCT 
TTAACTCCAT CCTGGGCGAC TCCTTCTTCC TCCATCTCCT CCTGTTCTGC TCTGCAGCCC 
TCCTGGCTCT CCTCCACCTA CACCACTCAT CCTCTTCTCT CCTCCTCCAT CTCCTCCTCC 
TCTCATCTCC TGTCCTCCAC TGTGTTCTCC TCCTCCTCTC TGCTCAGCAC TAAGAACACC 
CAGCTGAAGC GGGATCCCCT GTCCTCCTCT CTCTGCTCTC CTCCCTCCAC CCTCCCTCTG 
CTCCACAGCA CATCTCTCTC TGCTGCTGTA GCTGATCCTC CTCAGCCAGA GCAGCTGAGT 
GTGGCTTTAA AAAAGAGGAA AATCGAGCTG CAAAGTCTGA GCTCCAACGA TTACACCACA 
TCACAAATCG AGGAGCTGAA CGAGGATTTA GAGAATGAGG ACCTGGTAGA GATGGACTCT 
GGGCACACAG AGTTGCCTGT GCTGGACCTG AGACTGAGTG ATGAAGAGGA GAAGCAGACA 
GTGGATGTTG GGGAGGTGTT CACTGTGTCC AGCACTGACT CAGACTGGAC TGTAGAGTTA 
CTAAAGGAAA AGAAGTATGT TCTGCTGAAT GTGGTGTGCT GCTCCCTGGT GAATAAAAGC 
AGTGCTCCGA ACCAGAACGA CTGGGACAGC AAGGAAAATG TTTGGACACG GATAAAAGAC 
CTCGGCACAC AGATCACCCG CAGAGACCCT GAGTTCATCC TTAAGGTGGC CGTCTACACC 
AGGCAGGAGC TCAACATCCG CATCACCACC AACTTCCTGC TGGCCCTGGC GGCCTACCTG 
CCAGAGTCTA AACCCCACCT GAGGAGGTAC TTCTGTGCAG CGGTGCAGCT GCCCTCTGAT 
TGGCTGGAAG TGACCAGACT GTTCAGCACG TGTTTCAGCT CGTCTCTCCC AGCATGCCTG 
AAAAAAGCCC TGGTGGACAA ATTCAAGCAG TTCAGCGAGT ATCAGCTGGC CAAGTACAAC 
ACCCGCAAAC TCCGCGGCAA ACACAACAAG AAGAAGCGTG AAGTCAAGGA GTCGACCCCT 
GAGCAATGGG AGAAGTGGGC ACAGATGCTG AAAGTAGAGT CTGACGTTAT GAAGAAAATG 
GTGGAGATGT CTGAGCGTCC TGCAGTGGAT AAAAAGCAGA GCGAGTTCAA CATGAAGAAG 
ATGATAAAAC GCCTTCATAT TAAAGAGCCT GCAGAGTTTG TGATGGGCAT TCTGGGAAAG 
AGGTACCCGT CTGATCTGAG GGCGTTTGTG AGCAGTGGTT TAAGTGGTGT GTGGGAGAGC 
GAGCGAGCCG GTCAGCGTAT GAAGCTGAAG CAGCCGGACA CCTGGGAGAC CAAACTCAGT 
CTGGAGGGAA ACAACGCGGC TGTGTGGGAG AAACTCATAG ACAATAACTC TCTACCCTTC 
ATGGCCATGC TGAGGAACCT GAGGAACATG ATCACTCAAG GGATCAGCGA CCAGCACCAT 
GAACGCATAC TGAAACGACT GACCTCAAAG CTTGAGGAGG CGGCGGTGCT GCTGGCCCTG 
ATGATCAGCC ACTGCTGTGA GCACCCGATG TTTCTGATAC ACGACTTCGA TAACATACAT 
GAAGTCAAGC TCAAATCCGA CGTTTTCCTG GAGAATGTGA AACATGTGAT GAAGGAGCTC 
TCGGAGATAA ATGATTCTTC TGAAAAGATT CTGCTTGAAA GCTTCTTCTT CAGGCTGACT 
GAAGAGAAAA CAAAGGTGGA TAATATAATC GTGCTGAATG ATGTGAGGAT GTATTATTCA 
GTAGATCAGG CTGTGGAGAA GTACAGGAAG GAGTCGCTCA GTGATCCTCT ACTGGTCAAT 
ATCATGATGA GATCCAGTAT GAATAATTGT CATTCTGAGG GTGGAGACCC CAGAAGTGTG 
TATCTGAGTG GGTTTAGTGA GCAGATCCTC AGGTTTGTTT CAGAAAGAGG CTCCTCCAGG 
TTTTTGGATC ATGTAGAGAG CATCGACAGG CTGCGCAACA TCCCCCCACC AGCAGGTGGC 
TCCACCAAAC CTGAGAGAGC AGCCGACATC ACCCCATTGC CCGTCACTCC TAAACTCAGG 
TGGCAAGGGG TTCGTGTTTT TATCTCCTCT ACGTTCAGGG ACATGCATGC TGAGCGGGAC 
GTCCTGGTGC GGAGCGTGTT TCCGGAGCTG CGGCGCCGTG CAGCTCCACA CTGCCTCTAC 
CTGCAGGAGG TGGAACTGCG CTGGGGCGTA ACCGAGGAGG AGTCTAACCA GGCAATCCAG 
CTCTGTCTGT CCGAGGTGTG CCGCAGCCAG CTGCTGCTCA GCATTCTGGG AGAGAGATAC 
GGGCAGGTGC CACCTCGACC TACACTTCCT GAGCTTCCTC AGTACACCTG GCTGAACTCG 
GCCCCTGATG GGATGTCGAT CACAGAGATG GAGATTCGTC AGTTTCAGGC TGTGTATCCA 
GACTCCGCCC AGGGCAGAAT GTTCTTCTAC TTCAGATCTC CTCACCTGGC CAGTTCTGTT 
CCTGTGGAGT GGAGAGAGGA CTTCACGTCC GAGTCGAGCC AATCAGCAGC TAAAATGAGC 
GATCTGAAAG AATGGATCCG GAGCAACCAG TTCAGAGTCA CAGAGGATTA CCCGTGTGAG 
TGGGGCGGGG CTGTGGACGG AAGGCCGTAT GTGAAAGGTC TGGAGGATTT TGCGAAAGCA 
GCTCTGGAGG ATCTGTGGGA AGCCCTGGAG AAACTCTTTG TGGAGGAAGT AGACGAGGCT 
GATCTGATGT CGGAGATTAA TGAACAGGAG GTTCATCAGG ACGCTCAGCA GCGGCATTTC 
TACGGCCGGA AGAAACTGGT TTCCTCAGCA ATAGAGAAAA TTCAGGAGTG CCAGCAGAAA 
GGAGGCATCC TGCTGGTGGA GGGAGCTCAG GGAGAGGGCA AGACCGCCTT TATGGCTGGT 
CTGGCTCACG CGCTCCAAAC TCCAGATAAG CGCAGAAAAG TGCCAGTTTG TGATGTCATA 
TCCTATTCCA CAGAGGCTAG CCAATCAGCT TCTGGCGTAG AGCACCTCCT ACGCTGCCTC 
ATCCAATGGC TGAGGAGGAG GAAACAACAG GAGGAGGAGT TAGTCACTAG CACACCTTAC 
AGGGAGCTGC TGTGTGAGTT CCTAGTTCAG ATATCTGAGC TGAAGAGAGG GAATCCTCTA 
GTGATTCTGG TGGACGGTGC GGACGTTGTG CACGATCCCA GAGGACAAGC TGTTTCTGAA 
TGGATACCAC AACACATTCC CAAGGGTGTC TGTCTGGTTC TGAGTGTCAC CAGAGGCTCC 
GCCCTCCGCA CCACCCTCTC AGGTAAGCAG GGGTGTGTGG TGTTCGGGAT GGGTCAGTTA 
TCTCTGCCGG ACAGGAAGGA GATTGTGCAG AAGGAGCTGA GTGTGTACGG GAAGAAGCTG 
AGTGACTCTG CCTTTAATAA CCAGCTTCAG ACGCTGCTGA TGAAGAAAGG AGCCGTCAGC 
CCACTCTACC TCCACTTCAC CTGCGAGGAG CTGAGGAACT ACGCCTCCTT CGAAACGATG 
AAGAGGAACC TGCAGACTCT TCCTCCGTCT CTGTGTGAGC TGGTGCAGCA CAGTCTGCTC 
AGGCTGCAGT CTCAATACGG GGCGGCGGGG CTGGACTGGA CCCTGGCGGC GCTGACCGTC 
AGCTCTACAG GTCTGAGGGA GAGAGATCTG TACGCTGTGC TGAACCTCTG CAGTGACCTG 
ATCTCCACAC AGGGTCAGCT CACCTGGAAC AGAGCGCTCA CAGCGGCCCG GAGCCCGAGG 
AGCCGTGTGC CCATGGCGCT GTTCTCTCAG CTGGCACAGG GTTTACAGGG TTTGATCCGT 
CCGTCTCTCT CTCATGAGCC GGACGAGCCC CTGACCCTGA CCCACCCGGA TGTGAAGCTG 
GCGTTTGAGC AGCTGTATCT CTCCACTGAT CAGGACAGAG CCAGAGCACA CCTATTACTG 
GCAGGTGATC TTAAGGAGCT TCAGGCCTGT CACTCATTTC TGAAGCGCCA CGCCCCTCTC 
CTGTCCTGCT GGCCGCGGCT GTTTGTGCAG CAGGCTCTGA ACGAAGCGGC CGGCTCGTCT 
GCTCACGCCT GGGCTCGCAC GCTGGTGGAG GAGGGAGGAG TGAACGCAGT CAGGAACCTG 
AACAACACTG ACGGCCCTCA GCTGGAAACT GGAGAGCTGG TGTCCACCTT CCAGGCCAAG 
CCGTCCTGTG TGGCTCTGAG TCCGGGGGGA GGGTGTGTGG CTGTGGGGAC GGAGCAGGGA 
ACCATACACT TCATCCACAC ACACACCAAC CAGGAAGTGA AGTCACTGGT CAGTAACTGT 
GATGGCATCT CCAGCTGTGT GTTTCTGGAT GACGGATTTC TAAGCACCAC ATCATATGAT 
GGGCAGGTGG AGGTGTGGGA TATTAACAGT GGCAGCAGGA CTGTCCACCT GAACGCCCAC 
TCCAACAGGA TCACAGGATG TGATGTCAGC TCAGATAAGA AGCACTTTGC CACAGTCTCT 
TTAGACTCCA ATCTGAAGGT GTGGTCGTCT CAGAAGGGAA AGCAGGTGGT GTCTCTGCTC 
ACCCCCTGCC CTCTAAACTG TGTGACCTTT GACCCTGAGG GAGGTCTGCT GGCGGTGGGC 
TGCTGGGACA GAACGGTGCG AGTGTGGAAC TGGATCAAAC AGGAAAGATG GACGACTCTC 
TCAGGTCATC AGTCGTCGGT GCGCAGTGTC TCGTTCTCTC CCTCCTCCTC TCTGCTGTGC 
TCTGGTAGTC TGGATGGTGA GGTGCTTCTG TGGTCATCCT CTGGCTTGGC CTGTGTGGGG 
CGCTATCAGG CTCACCGTGG CTCTACTGAG GCCCTGAGTT TCCTGTCTGG AGGAGAAGCA 
CTGCTGAGTG CTGGACACGA TGGCATGGTT CAGTTGTGGT CTGGAGGTCT GGGGTGCTCT 
GTTTCTGTGC TGGGAGAGGA AACGAAAGCG GTGAAGTCGA GTGTGTCCAG AGCTGCAGCA 
GTCTCTGAAA GTGCCGCTCT CTCTGTGGCT GGAGCTCATG GATACGCTGC TGTGGGATAT 
CATGGAGATG GTATCAGACT GTTTGAGCTG AAATCAGGTG AGAGGGTGTG GTCCACTGAG 
GACCTGCGGG TGTCTGTGCC GTGCCTGCTG TGGATGGAGG CTGCTGGGGT GGATCTGCTT 
TTGTCTGGAG CTTTAGACAG TGGTCTCCGG CTGTGGAGCA GGCAGGGGGC TGGTATGACC 
CTCAGGGGGG CTTTTGGGGT ACAGAAGGGG GGGATCCTCT CATTGGCTCA GAGCTCTGTG 
TACGTGGCGT CTGCTTCAGA CGACTTCACC ATCGCTCTGT GGCTGAAGAG TGACCTGACC 
TCTGACCCCT GGGTTCAGCC CAGTGCGGTT AGTGTACTGA TGGGTCACAG TGGAGGGGTC 
ACGTGTCTGG CCTTCAGTCC AGACGGAGAC CATTTACTGT CTGGAGGCAA AGACAAGTAT 
GACACAGAGA GATTGATTAA CACTCTGGTC TGTCTCTCAT GCTCTCTGCA GGTTCAGCAC 
CGCAGCACTC TGCTGGGTCA CAGCGGTGCT GTCCAGGCGC TGGCGAGTGG ACGAGGTCCA 
CAGTTCCTCA CGGTGTCTGA GGACCGCTCT CTCCGAGCCT GGAGCGTTCG TACAGAGAAT 
CCTCCCTTTC AGGAAGAGTG TGTGAGTGCA GTGTGTTTTC TGGATTCAGA GAAGCTGCTG 
GTGTGTGGTT ATACCTCAGG AAAACTGGAG ATATGGCACA ACACTTTAAA GATCTACTCC 
AGTAAGGTGA GCGACAGTCG TATTCGAGCG GTGAGCGGAA TGCCGGATCA GAGCCTGGCT 
GTGGGCTGCA GTGATTACAC TGTGACTGTC TTTAAACTGA CCAGAGACCA TCAGCACTGC 
ACTGCCAGTT TGACTAAAGG ATCGTTGTAT ACTTTGGAGA AGGGTGTGAA TTTCCTGTGT 
TACTGTAACA CTCTGATGGG AGTGTGTGTG GACAGTTCTG TCATCGACAT CTTCTCCACT 
GTGACCGACA ATACAAGAAT GATCGACACG TGGCAGTACA CCATGCGGAT TATGGGCGTT 
TTCAAGAGTG ATGTAAACAG CCTGTGGCTG CTGGGAGAGG AAAACGACAA CATCCACCTT 
TCATACCTGC TCTGTGCCAG TGCGGAACTG ACCTCAAATT CAACGACTAT GAGTGCGAAA 
GTGGGAGGGG ACAAGGACAG TGATGATGAA GATGAGAAGG ACACGGAGAA AGAGCAGACT 
TTCACCATTA CAGCTGCTAC CATGCACAAC AATTTTATCG TGTGTGGAGA CTCTAAAGGC 
AATATGTGGT TCAACAATGT GTCGAACACG GACAGCTGGA GCCCAAGAAG ACCCGTTCAC 
AGTGACAGGA TCAGTGTTCT GCGGCTGACG GACAGCACTA TAATCTCCGC CTCTCACGAC 
AGAACCGTCA AACTGTGGGA CAGAACCACC AACAAACAGG TGGGTCTGTT TGTGTGTGCT 
GCTGCGGTGG AGGTGATGGA GGTGGACCCC GCGGACCCCT GCAGGCTGGT GTGTGGAGAT 
GCGCTGGGGA AGCTCTACTT CCTGTCCTGG AAAGAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015460250.1
CDS	173..6871
Translation	MEAATLIPSDPKVNPLIHSDPKVNSEAFNYCNSGPNLLLQKGMENRILAQASSLLPHAPALTPSWATPSSSISSCSALQPSWLSSTYTTHPLLSSSISSSSHLLSSTVFSSSSLLSTKNTQLKRDPLSSSLCSPPSTLPLLHSTSLSAAVADPPQPEQLSVALKKRKIELQSLSSNDYTTSQIEELNEDLENEDLVEMDSGHTELPVLDLRLSDEEEKQTVDVGEVFTVSSTDSDWTVELLKEKKYVLLNVVCCSLVNKSSAPNQNDWDSKENVWTRIKDLGTQITRRDPEFILKVAVYTRQELNIRITTNFLLALAAYLPESKPHLRRYFCAAVQLPSDWLEVTRLFSTCFSSSLPACLKKALVDKFKQFSEYQLAKYNTRKLRGKHNKKKREVKESTPEQWEKWAQMLKVESDVMKKMVEMSERPAVDKKQSEFNMKKMIKRLHIKEPAEFVMGILGKRYPSDLRAFVSSGLSGVWESERAGQRMKLKQPDTWETKLSLEGNNAAVWEKLIDNNSLPFMAMLRNLRNMITQGISDQHHERILKRLTSKLEEAAVLLALMISHCCEHPMFLIHDFDNIHEVKLKSDVFLENVKHVMKELSEINDSSEKILLESFFFRLTEEKTKVDNIIVLNDVRMYYSVDQAVEKYRKESLSDPLLVNIMMRSSMNNCHSEGGDPRSVYLSGFSEQILRFVSERGSSRFLDHVESIDRLRNIPPPAGGSTKPERAADITPLPVTPKLRWQGVRVFISSTFRDMHAERDVLVRSVFPELRRRAAPHCLYLQEVELRWGVTEEESNQAIQLCLSEVCRSQLLLSILGERYGQVPPRPTLPELPQYTWLNSAPDGMSITEMEIRQFQAVYPDSAQGRMFFYFRSPHLASSVPVEWREDFTSESSQSAAKMSDLKEWIRSNQFRVTEDYPCEWGGAVDGRPYVKGLEDFAKAALEDLWEALEKLFVEEVDEADLMSEINEQEVHQDAQQRHFYGRKKLVSSAIEKIQECQQKGGILLVEGAQGEGKTAFMAGLAHALQTPDKRRKVPVCDVISYSTEASQSASGVEHLLRCLIQWLRRRKQQEEELVTSTPYRELLCEFLVQISELKRGNPLVILVDGADVVHDPRGQAVSEWIPQHIPKGVCLVLSVTRGSALRTTLSGKQGCVVFGMGQLSLPDRKEIVQKELSVYGKKLSDSAFNNQLQTLLMKKGAVSPLYLHFTCEELRNYASFETMKRNLQTLPPSLCELVQHSLLRLQSQYGAAGLDWTLAALTVSSTGLRERDLYAVLNLCSDLISTQGQLTWNRALTAARSPRSRVPMALFSQLAQGLQGLIRPSLSHEPDEPLTLTHPDVKLAFEQLYLSTDQDRARAHLLLAGDLKELQACHSFLKRHAPLLSCWPRLFVQQALNEAAGSSAHAWARTLVEEGGVNAVRNLNNTDGPQLETGELVSTFQAKPSCVALSPGGGCVAVGTEQGTIHFIHTHTNQEVKSLVSNCDGISSCVFLDDGFLSTTSYDGQVEVWDINSGSRTVHLNAHSNRITGCDVSSDKKHFATVSLDSNLKVWSSQKGKQVVSLLTPCPLNCVTFDPEGGLLAVGCWDRTVRVWNWIKQERWTTLSGHQSSVRSVSFSPSSSLLCSGSLDGEVLLWSSSGLACVGRYQAHRGSTEALSFLSGGEALLSAGHDGMVQLWSGGLGCSVSVLGEETKAVKSSVSRAAAVSESAALSVAGAHGYAAVGYHGDGIRLFELKSGERVWSTEDLRVSVPCLLWMEAAGVDLLLSGALDSGLRLWSRQGAGMTLRGAFGVQKGGILSLAQSSVYVASASDDFTIALWLKSDLTSDPWVQPSAVSVLMGHSGGVTCLAFSPDGDHLLSGGKDKYDTERLINTLVCLSCSLQVQHRSTLLGHSGAVQALASGRGPQFLTVSEDRSLRAWSVRTENPPFQEECVSAVCFLDSEKLLVCGYTSGKLEIWHNTLKIYSSKVSDSRIRAVSGMPDQSLAVGCSDYTVTVFKLTRDHQHCTASLTKGSLYTLEKGVNFLCYCNTLMGVCVDSSVIDIFSTVTDNTRMIDTWQYTMRIMGVFKSDVNSLWLLGEENDNIHLSYLLCASAELTSNSTTMSAKVGGDKDSDDEDEKDTEKEQTFTITAATMHNNFIVCGDSKGNMWFNNVSNTDSWSPRRPVHSDRISVLRLTDSTIISASHDRTVKLWDRTTNKQVGLFVCAAAVEVMEVDPADPCRLVCGDALGKLYFLSWKE

Target ORF information:

RefSeq Version	XM_015604764.1
Organism	Astyanax mexicanus(Mexican tetra)
Definition	Astyanax mexicanus telomerase protein component 1-like (LOC103036747), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015604764.1

ORF Insert Sequence:

ATGGAGGCGG CGACCCTGAT CCCCTCCGAC CCCAAGGTCA ATCCGCTGAT CCACTCCGAC 
CCCAAGGTCA ATTCAGAGGC CTTTAATTAT TGCAACAGTG GCCCAAATCT GCTGTTACAA 
AAAGGTATGG AGAACAGGAT CCTGGCCCAG GCCTCTTCTC TTCTGCCCCA CGCTCCTGCT 
TTAACTCCAT CCTGGGCGAC TCCTTCTTCC TCCATCTCCT CCTGTTCTGC TCTGCAGCCC 
TCCTGGCTCT CCTCCACCTA CACCACTCAT CCTCTTCTCT CCTCCTCCAT CTCCTCCTCC 
TCTCATCTCC TGTCCTCCAC TGTGTTCTCC TCCTCCTCTC TGCTCAGCAC TAAGAACACC 
CAGCTGAAGC GGGATCCCCT GTCCTCCTCT CTCTGCTCTC CTCCCTCCAC CCTCCCTCTG 
CTCCACAGCA CATCTCTCTC TGCTGCTGTA GCTGATCCTC CTCAGCCAGA GCAGCTGAGT 
GTGGCTTTAA AAAAGAGGAA AATCGAGCTG CAAAGTCTGA GCTCCAACGA TTACACCACA 
TCACAAATCG AGGAGCTGAA CGAGGATTTA GAGAATGAGG ACCTGGTAGA GATGGACTCT 
GGGCACACAG AGTTGCCTGT GCTGGACCTG AGACTGAGTG ATGAAGAGGA GAAGCAGACA 
GTGGATGTTG GGGAGGTGTT CACTGTGTCC AGCACTGACT CAGACTGGAC TGTAGAGTTA 
CTAAAGGAAA AGAAGTATGT TCTGCTGAAT GTGGTGTGCT GCTCCCTGGT GAATAAAAGC 
AGTGCTCCGA ACCAGAACGA CTGGGACAGC AAGGAAAATG TTTGGACACG GATAAAAGAC 
CTCGGCACAC AGATCACCCG CAGAGACCCT GAGTTCATCC TTAAGGTGGC CGTCTACACC 
AGGCAGGAGC TCAACATCCG CATCACCACC AACTTCCTGC TGGCCCTGGC GGCCTACCTG 
CCAGAGTCTA AACCCCACCT GAGGAGGTAC TTCTGTGCAG CGGTGCAGCT GCCCTCTGAT 
TGGCTGGAAG TGACCAGACT GTTCAGCACG TGTTTCAGCT CGTCTCTCCC AGCATGCCTG 
AAAAAAGCCC TGGTGGACAA ATTCAAGCAG TTCAGCGAGT ATCAGCTGGC CAAGTACAAC 
ACCCGCAAAC TCCGCGGCAA ACACAACAAG AAGAAGCGTG AAGTCAAGGA GTCGACCCCT 
GAGCAATGGG AGAAGTGGGC ACAGATGCTG AAAGTAGAGT CTGACGTTAT GAAGAAAATG 
GTGGAGATGT CTGAGCGTCC TGCAGTGGAT AAAAAGCAGA GCGAGTTCAA CATGAAGAAG 
ATGATAAAAC GCCTTCATAT TAAAGAGCCT GCAGAGTTTG TGATGGGCAT TCTGGGAAAG 
AGGTACCCGT CTGATCTGAG GGCGTTTGTG AGCAGTGGTT TAAGTGGTGT GTGGGAGAGC 
GAGCGAGCCG GTCAGCGTAT GAAGCTGAAG CAGCCGGACA CCTGGGAGAC CAAACTCAGT 
CTGGAGGGAA ACAACGCGGC TGTGTGGGAG AAACTCATAG ACAATAACTC TCTACCCTTC 
ATGGCCATGC TGAGGAACCT GAGGAACATG ATCACTCAAG GGATCAGCGA CCAGCACCAT 
GAACGCATAC TGAAACGACT GACCTCAAAG CTTGAGGAGG CGGCGGTGCT GCTGGCCCTG 
ATGATCAGCC ACTGCTGTGA GCACCCGATG TTTCTGATAC ACGACTTCGA TAACATACAT 
GAAGTCAAGC TCAAATCCGA CGTTTTCCTG GAGAATGTGA AACATGTGAT GAAGGAGCTC 
TCGGAGATAA ATGATTCTTC TGAAAAGATT CTGCTTGAAA GCTTCTTCTT CAGGCTGACT 
GAAGAGAAAA CAAAGGTGGA TAATATAATC GTGCTGAATG ATGTGAGGAT GTATTATTCA 
GTAGATCAGG CTGTGGAGAA GTACAGGAAG GAGTCGCTCA GTGATCCTCT ACTGGTCAAT 
ATCATGATGA GATCCAGTAT GAATAATTGT CATTCTGAGG GTGGAGACCC CAGAAGTGTG 
TATCTGAGTG GGTTTAGTGA GCAGATCCTC AGGTTTGTTT CAGAAAGAGG CTCCTCCAGG 
TTTTTGGATC ATGTAGAGAG CATCGACAGG CTGCGCAACA TCCCCCCACC AGCAGGTGGC 
TCCACCAAAC CTGAGAGAGC AGCCGACATC ACCCCATTGC CCGTCACTCC TAAACTCAGG 
TGGCAAGGGG TTCGTGTTTT TATCTCCTCT ACGTTCAGGG ACATGCATGC TGAGCGGGAC 
GTCCTGGTGC GGAGCGTGTT TCCGGAGCTG CGGCGCCGTG CAGCTCCACA CTGCCTCTAC 
CTGCAGGAGG TGGAACTGCG CTGGGGCGTA ACCGAGGAGG AGTCTAACCA GGCAATCCAG 
CTCTGTCTGT CCGAGGTGTG CCGCAGCCAG CTGCTGCTCA GCATTCTGGG AGAGAGATAC 
GGGCAGGTGC CACCTCGACC TACACTTCCT GAGCTTCCTC AGTACACCTG GCTGAACTCG 
GCCCCTGATG GGATGTCGAT CACAGAGATG GAGATTCGTC AGTTTCAGGC TGTGTATCCA 
GACTCCGCCC AGGGCAGAAT GTTCTTCTAC TTCAGATCTC CTCACCTGGC CAGTTCTGTT 
CCTGTGGAGT GGAGAGAGGA CTTCACGTCC GAGTCGAGCC AATCAGCAGC TAAAATGAGC 
GATCTGAAAG AATGGATCCG GAGCAACCAG TTCAGAGTCA CAGAGGATTA CCCGTGTGAG 
TGGGGCGGGG CTGTGGACGG AAGGCCGTAT GTGAAAGGTC TGGAGGATTT TGCGAAAGCA 
GCTCTGGAGG ATCTGTGGGA AGCCCTGGAG AAACTCTTTG TGGAGGAAGT AGACGAGGCT 
GATCTGATGT CGGAGATTAA TGAACAGGAG GTTCATCAGG ACGCTCAGCA GCGGCATTTC 
TACGGCCGGA AGAAACTGGT TTCCTCAGCA ATAGAGAAAA TTCAGGAGTG CCAGCAGAAA 
GGAGGCATCC TGCTGGTGGA GGGAGCTCAG GGAGAGGGCA AGACCGCCTT TATGGCTGGT 
CTGGCTCACG CGCTCCAAAC TCCAGATAAG CGCAGAAAAG TGCCAGTTTG TGATGTCATA 
TCCTATTCCA CAGAGGCTAG CCAATCAGCT TCTGGCGTAG AGCACCTCCT ACGCTGCCTC 
ATCCAATGGC TGAGGAGGAG GAAACAACAG GAGGAGGAGT TAGTCACTAG CACACCTTAC 
AGGGAGCTGC TGTGTGAGTT CCTAGTTCAG ATATCTGAGC TGAAGAGAGG GAATCCTCTA 
GTGATTCTGG TGGACGGTGC GGACGTTGTG CACGATCCCA GAGGACAAGC TGTTTCTGAA 
TGGATACCAC AACACATTCC CAAGGGTGTC TGTCTGGTTC TGAGTGTCAC CAGAGGCTCC 
GCCCTCCGCA CCACCCTCTC AGGTAAGCAG GGGTGTGTGG TGTTCGGGAT GGGTCAGTTA 
TCTCTGCCGG ACAGGAAGGA GATTGTGCAG AAGGAGCTGA GTGTGTACGG GAAGAAGCTG 
AGTGACTCTG CCTTTAATAA CCAGCTTCAG ACGCTGCTGA TGAAGAAAGG AGCCGTCAGC 
CCACTCTACC TCCACTTCAC CTGCGAGGAG CTGAGGAACT ACGCCTCCTT CGAAACGATG 
AAGAGGAACC TGCAGACTCT TCCTCCGTCT CTGTGTGAGC TGGTGCAGCA CAGTCTGCTC 
AGGCTGCAGT CTCAATACGG GGCGGCGGGG CTGGACTGGA CCCTGGCGGC GCTGACCGTC 
AGCTCTACAG GTCTGAGGGA GAGAGATCTG TACGCTGTGC TGAACCTCTG CAGTGACCTG 
ATCTCCACAC AGGGTCAGCT CACCTGGAAC AGAGCGCTCA CAGCGGCCCG GAGCCCGAGG 
AGCCGTGTGC CCATGGCGCT GTTCTCTCAG CTGGCACAGG GTTTACAGGG TTTGATCCGT 
CCGTCTCTCT CTCATGAGCC GGACGAGCCC CTGACCCTGA CCCACCCGGA TGTGAAGCTG 
GCGTTTGAGC AGCTGTATCT CTCCACTGAT CAGGACAGAG CCAGAGCACA CCTATTACTG 
GCAGGTGATC TTAAGGAGCT TCAGGCCTGT CACTCATTTC TGAAGCGCCA CGCCCCTCTC 
CTGTCCTGCT GGCCGCGGCT GTTTGTGCAG CAGGCTCTGA ACGAAGCGGC CGGCTCGTCT 
GCTCACGCCT GGGCTCGCAC GCTGGTGGAG GAGGGAGGAG TGAACGCAGT CAGGAACCTG 
AACAACACTG ACGGCCCTCA GCTGGAAACT GGAGAGCTGG TGTCCACCTT CCAGGCCAAG 
CCGTCCTGTG TGGCTCTGAG TCCGGGGGGA GGGTGTGTGG CTGTGGGGAC GGAGCAGGGA 
ACCATACACT TCATCCACAC ACACACCAAC CAGGAAGTGA AGTCACTGGT CAGTAACTGT 
GATGGCATCT CCAGCTGTGT GTTTCTGGAT GACGGATTTC TAAGCACCAC ATCATATGAT 
GGGCAGGTGG AGGTGTGGGA TATTAACAGT GGCAGCAGGA CTGTCCACCT GAACGCCCAC 
TCCAACAGGA TCACAGGATG TGATGTCAGC TCAGATAAGA AGCACTTTGC CACAGTCTCT 
TTAGACTCCA ATCTGAAGGT GTGGTCGTCT CAGAAGGGAA AGCAGGTGGT GTCTCTGCTC 
ACCCCCTGCC CTCTAAACTG TGTGACCTTT GACCCTGAGG GAGGTCTGCT GGCGGTGGGC 
TGCTGGGACA GAACGGTGCG AGTGTGGAAC TGGATCAAAC AGGAAAGATG GACGACTCTC 
TCAGGTCATC AGTCGTCGGT GCGCAGTGTC TCGTTCTCTC CCTCCTCCTC TCTGCTGTGC 
TCTGGTAGTC TGGATGGTGA GGTGCTTCTG TGGTCATCCT CTGGCTTGGC CTGTGTGGGG 
CGCTATCAGG CTCACCGTGG CTCTACTGAG GCCCTGAGTT TCCTGTCTGG AGGAGAAGCA 
CTGCTGAGTG CTGGACACGA TGGCATGGTT CAGTTGTGGT CTGGAGGTCT GGGGTGCTCT 
GTTTCTGTGC TGGGAGAGGA AACGAAAGCG GTGAAGTCGA GTGTGTCCAG AGCTGCAGCA 
GTCTCTGAAA GTGCCGCTCT CTCTGTGGCT GGAGCTCATG GATACGCTGC TGTGGGATAT 
CATGGAGATG GTATCAGACT GTTTGAGCTG AAATCAGGTG AGAGGGTGTG GTCCACTGAG 
GACCTGCGGG TGTCTGTGCC GTGCCTGCTG TGGATGGAGG CTGCTGGGGT GGATCTGCTT 
TTGTCTGGAG CTTTAGACAG TGGTCTCCGG CTGTGGAGCA GGCAGGGGGC TGGTATGACC 
CTCAGGGGGG CTTTTGGGGT ACAGAAGGGG GGGATCCTCT CATTGGCTCA GAGCTCTGTG 
TACGTGGCGT CTGCTTCAGA CGACTTCACC ATCGCTCTGT GGCTGAAGAG TGACCTGACC 
TCTGACCCCT GGGTTCAGCC CAGTGCGGTT AGTGTACTGA TGGGTCACAG TGGAGGGGTC 
ACGTGTCTGG CCTTCAGTCC AGACGGAGAC CATTTACTGT CTGGAGGCAA AGACAAGTAT 
GACACAGAGA GATTGATTAA CACTCTGGTC TGTCTCTCAT GCTCTCTGCA GGTTCAGCAC 
CGCAGCACTC TGCTGGGTCA CAGCGGTGCT GTCCAGGCGC TGGCGAGTGG ACGAGGTCCA 
CAGTTCCTCA CGGTGTCTGA GGACCGCTCT CTCCGAGCCT GGAGCGTTCG TACAGAGAAT 
CCTCCCTTTC AGGAAGAGTG TGTGAGTGCA GTGTGTTTTC TGGATTCAGA GAAGCTGCTG 
GTGTGTGGTT ATACCTCAGG AAAACTGGAG ATATGGCACA ACACTTTAAA GATCTACTCC 
AGTAAGGTGA GCGACAGTCG TATTCGAGCG GTGAGCGGAA TGCCGGATCA GAGCCTGGCT 
GTGGGCTGCA GTGATTACAC TGTGACTGTC TTTAAACTGA CCAGAGACCA TCAGCACTGC 
ACTGCCAGTT TGACTAAAGG ATCGTTGTAT ACTTTGGAGA AGGGTGTGAA TTTCCTGTGT 
TACTGTAACA CTCTGATGGG AGTGTGTGTG GACAGTTCTG TCATCGACAT CTTCTCCACT 
GTGACCGACA ATACAAGAAT GATCGACACG TGGCAGTACA CCATGCGGAT TATGGGCGTT 
TTCAAGAGTG ATGTAAACAG CCTGTGGCTG CTGGGAGAGG AAAACGACAA CATCCACCTT 
TCATACCTGC TCTGTGCCAG TGCGGAACTG ACCTCAAATT CAACGACTAT GAGTGCGAAA 
GTGGGAGGGG ACAAGGACAG TGATGATGAA GATGAGAAGG ACACGGAGAA AGAGCAGACT 
TTCACCATTA CAGCTGCTAC CATGCACAAC AATTTTATCG TGTGTGGAGA CTCTAAAGGC 
AATATGTGGT TCAACAATGT GTCGAACACG GACAGCTGGA GCCCAAGAAG ACCCGTTCAC 
AGTGACAGGA TCAGTGTTCT GCGGCTGACG GACAGCACTA TAATCTCCGC CTCTCACGAC 
AGAACCGTCA AACTGTGGGA CAGAACCACC AACAAACAGG TGGGTCTGTT TGTGTGTGCT 
GCTGCGGTGG AGGTGATGGA GGTGGACCCC GCGGACCCCT GCAGGCTGGT GTGTGGAGAT 
GCGCTGGGGA AGCTCTACTT CCTGTCCTGG AAAGAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

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tep1 cDNA ORF clone, Astyanax mexicanus(Mexican tetra)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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