LOC103033153 cDNA ORF clone, Astyanax mexicanus(Mexican tetra)

Gene
Clones
gRNAs

Summary

Gene Symbol	LOC103033153
Entrez Gene ID	103033153
Full Name	tet methylcytosine dioxygenase 1
Gene Type	protein-coding
Organism	Astyanax mexicanus(Mexican tetra)

mRNA and Protein(s)

mRNA	Protein	Name
XM_007240272.2	XP_007240334.1	methylcytosine dioxygenase TET3-like isoform X1
XM_007240272.3	XP_007240334.2	methylcytosine dioxygenase TET1 isoform X1
XM_007240273.2	XP_007240335.1	methylcytosine dioxygenase TET3-like isoform X2
XM_007240273.3	XP_007240335.2	methylcytosine dioxygenase TET1 isoform X2
XM_007240274.2	XP_007240336.1	methylcytosine dioxygenase TET3-like isoform X3

Pathways from BioSystems
Gene Ontology

The following LOC103033153 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC103033153 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OAs24336	XM_007240274.2 Latest version!	Astyanax mexicanus methylcytosine dioxygenase TET3-like (LOC103033153), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OAs120915	XM_007240272.3 Latest version!	Astyanax mexicanus tet methylcytosine dioxygenase 1 (tet1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OAs120916	XM_007240273.3 Latest version!	Astyanax mexicanus tet methylcytosine dioxygenase 1 (tet1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OAs24334	XM_007240272.2 Latest version!	Astyanax mexicanus methylcytosine dioxygenase TET3-like (LOC103033153), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OAs24335	XM_007240273.2 Latest version!	Astyanax mexicanus methylcytosine dioxygenase TET3-like (LOC103033153), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00

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Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LOC103033153 ( XM_007240274.2 ) cDNA ORF clone, Astyanax mexicanus(Mexican tetra) -> XP_007240336.1 Astyanax mexicanus methylcytosine dioxygenase TET3-like (LOC103033153), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OAs24336
Clone ID Related Accession (Same CDS sequence)	XM_007240274.2
Accession Version	XM_007240274.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4389bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1455033600000
Organism	Astyanax mexicanus(Mexican tetra)
Product	methylcytosine dioxygenase TET3-like isoform X3
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_006739483.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Feb 11, 2016 this sequence version replaced XM_007240274.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Astyanax mexicanus Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGCCTA AAATTGAGGC TCTGCCTCTG AGGAGCGAGA AGGTGGTGTG CAAGAATATT 
CCCAAATCAC GCAAAAGGAA CAGAGTGTCC AAGGTTGACT CAGAGGGCAT CTTAGTAAAT 
GGCACTAATT CAGAGCAGAT GGAAGAGACC TGTGTGGTGC CAGAGGAAGA GGCGCGTGAC 
GTATCTCAAG CACACTCCGC TCCACCTGAC TCTCCCACGC CTCTCGGGCA CGACACTGAA 
CCAGCACCAG CCGAAAGAGC CCTTCAGCCT GCTGTCAGCC ACCATGCACT TCTTACTCAC 
CAGAGCTCTT TAAGCTCCCA CAATGGTGCT AATCATATGG AACCAAACAC TGAGGATCAC 
AGAGATCCCA CTGACCTGCA GTCTAAGTCT CAAAGGACTG TGAACATGCC CACCAAAAGC 
CAGACGCCTG AGGCAATTCC TGCACAGACA GTTCCTCTGA AGAAGATCAA GCTAGAAGAG 
CCTAGCACAG TGTCAGATCA ACACAGCACA TCCTACCTAA GCAGCAGCGA CTTCTACCAG 
GATGCTTTGT CTACTTTAGC AAATGTTGTA TGTTCAACCA TCTCTGATCT GAAGGGTTTG 
GAGGAGAAGA CAGTTGGTCC TCAGACCTCA AATGCCTGTA ATATTAAAAC TGAAAGAGAA 
GAAGAACCAT ATCAGAGTCA TTCACATCAG GAAAGGACTT CTAACAGTCC AGCAGGTGCT 
GATAACTCCC ATCCGAAAGA CCCAGTTCCG CTGACTAATA ATAGTGTCCA GTCTTTAGTT 
GAGCATAGGA ACATAAGCAT TGATCAGGCA ATAGCTATTG AGGCCTTGAC CCAGCTGGCA 
GCTACCCCAA AATCAGCTCC TGTCAAGGCA GATTCTAAAG TTCAGGACCC TCCACAAGAA 
TCTTGTAACA AATTAGCAAA TGTTTCATCA AGCAGGCAAA GCCCGGTCCA AGAGGCTAAA 
CCCATATCTG AGGTCATCTC CAATAAAGTC TCTGTAATCA ATTCACATCA GACTTCTGTA 
ATTTGCAGCC CTCTGAACAG ACAGGAAAAC CCCACCCACC CCAAACTCTC CTTACAGGAT 
CTTTTAATAG CTAGCTCATA TGATAAGCTC TTGCATAGTC CAGAGAATGG ACGAGTAAGT 
CAAGCACTTT GTAAAACAGA AACGTTAGAT GGCACTTTTA AGACTTTTAA GCATGTTGAA 
AGGACGCAGA CCTCAAGGAG GAGAGATGAG GAGGAAGTTG CAGCTCAGTT GGTGCAACTT 
GCATTCATAA TTCAATCAAG AACCAATCCC ATGTCTTCTG AGAACAGTCC CCCCAAAGGG 
ATGCCAGTAC AGACCATAAA ATACAACCAT CATGTCGTTG GACAAAATGT AAAAAAGCAA 
CGGAAACCAA GAACAACTCC CTCAAGTCCC AGGATATCAA AGAAAAAAGC CTCTGAGGTT 
GAGGGAGGCA ACCATAGGAT ACCACTAGCC AAAAGGACAC CCAATAGCAA ACCCCCTTTC 
AAGACAAAAG CCCAGCATGT GGCCTTGCAG GAAAAGCCCA AACCACAACC CAAGTGGAGC 
CCGTTTCTCC CACAGACTCA GATAGACCTG AAAAAATACC TAGCACAGGC TCATCATGAG 
AAACATCAGC TGTTCCATTT TAGCAGCAAG AGACAAGAGT TGGACATGCA GACTCTCAAG 
AGTTCCGGAA ATCAGAAAGC TTTTACTTAC AAAACAGACC ACGCAGCTCT GAGTCATTCC 
CATGTTAACC AATTCAGATC CAACGGACAT TGTCACAGTC TCACAAATGG TCATCTAGGT 
GCTACACATG GCCAGAAGTA TGAATGTGAA GAACATTTGA TTTCTCAGGT ATCGAAAACA 
TACAGTGTGC TAAATCATGG TGCTAATCCA CAAGCCCAGT ATGCCATGCC TAGTGTCCCT 
GGTGCAGATC CATTGCCAGG GAGTCCAGGG AGGTGCCATA AAGTAAACAG CCTAAGCTGT 
GAGCCGCGGC AGACTTCCTT TGGTCAGAAT GGACGTTACA AAGTGGACAC GGCTGGCGCT 
GTCACCGTTT TGTCTGTGTC AGCTGGAAAC ATGGAAAATG CTAACACTGA ATCCGTTGAA 
GAAAACACTC CCACCAAGCA TATGCTTAAC AGCTTTTTGG AATCTCCGTT GAAGTACTTG 
GACACTCCAA CCAAGAATCT AATCAACACA CCCTCCAAAA AAACTCCGGA GCTCCCCTCC 
TGTGACTGTA TGGAGCAAAT CATTGAAAAA GAGGAGGGTC CATTCTACAC ACACCTTGGC 
TCTGGGCCAA CTGTAGCAGC AGTCCGAGAG ATGATGGAGG ACAGGTATGG GGAGAAGGGC 
AAAGCAGTGC GTGTGGAAGT TGTAGTTTAT ACTGGGAGAG AAGGAAGGAG CTCCCAGGGC 
TGTCCAATAG CCAAATGGGT GATCCGTCGG GCCAGTGAGG AAGAGAAGCT GCTGTGCCTA 
GTGCGCCAGC GTGCAGGTCA CCGCTGTCAG AGCGCAGTAG TGGTAATCCT CATCCTGGCC 
TGGGAGGGGA TTCCACGCAA CATGGCCGAC AGACTGTACC AGGAGCTTAC CCAGACCCTC 
TATAAATATG GCTCGCCCAC CAGCCGCCGC TGCGCCCTTA ATGAAGATCG TACCTGTGCC 
TGTCAGGGGC TGGACCCAGA TACCTGTGGA GCGTCCTTCT CTTTCGGCTG CTCGTGGAGT 
ATGTATTTTA ACGGCTGCAA GTTTGCCCGG AGCAAAGTGC CTAGAAAGTT CCGGCTGCAA 
GGAGACTACC CAGAGGAGGA AGAAAAGATC GAAAGCAACC TCCTAAATCT GGCAACTGAT 
GTTGCACCTT TGTATAAGAA ACTTGCTCCT GAAGCCTATG AAAACCAGGT GGAGCAGGAG 
CAGTCAGGGC AAAACTGTCG GCTGGGCAGG AGAGAGGGTC GGCCATTTTC TGGTGTGACA 
GCATGTGTGG ACTTTTGTGC TCATGCTCAC AGGGACACAC ACAACATGCA CAACGGGAGT 
ACTGTGGTAT GCACTTTAAC TAAGGAGGAT AACCGTGCTG TAAGAAACAT TCCAGAGGAT 
GAGCAGCTGC ATGTCCTGCC TCTTTACAAG ATCTCGGAGA CTGATGAGTT CGGTCGTGTG 
GAGGGTCAGT GGGCTAAGAT ACAGACTGGA GCTCTGCAGG TCCTCTCTTC CTTCCCAAGA 
GAGGTGCGTC TGCAGGCAGA GCCCGTCAAG TCTGCCCGTA AAAGGAGACA AGAGGCCAAG 
AGAGAGCGAG AGAAGCAGAG AAACCAGGAG AAGAAGCAGG TCACACCAGT GAAAGTGAAG 
AACGAGCCCC TCAAAGGTTT TAAAAACTCT TCCTCTGAGC TTTCACCGTG TTTCAAAACA 
GAAGCATGTT CTCCAACTCC TTATTGTTCC CCAATGCGAC TACCTAGGAC TCCAGACAAG 
GGGAGCCACT TACTGGACTC GGCCAGTCCT TACAGGAGCA CATCATGTAC TCCTCCATTA 
AGCTCTACCA GAGACACAGC AGCACTTTCG TCCTCCCCTC ACACCGTCCT CCCTGTGCCA 
CAATATCAGA ATGTTGGAGA ACTGGAGCAG AGCAATGAAT CTTCCCTCCT CAGACAGAGA 
CCAGGGTTAG AAGCAGCAAG ACACAGCTCC CCTGAGCTCT CAGAACAGAA ACCATTCAGA 
AACCAGCAGC AGATGGGCGG ATCTTTGGGT TTCCGTGACA AATGTTTTGC AGTAAAACCG 
GAGCCTGAGG AGGTCCGTTG TTTTCAAGGA GGAGCTGCTC CCCGACTGCT CTCCCCTTCC 
CGGGCTGAGG GTCTCCACAG CCGCCTGAAC TTTCGCCAAG CACAGCCGAG CCTGGATGAC 
CACCACGGGC CGCCGGTCAC CCCAAGGCCC CTGACCCCTG AGGAGGTCAA GGCAGAAGAG 
GTGTGGTCAG ACAGCGAGCA CAACTTCCTA GATGGAGACA TAGGCGGGGT CGCGGTGGCC 
CCCTCTCACG GCTCCATCCT TATCGAGTGT GCCCGGCGGG AGCTGCACGC TACCACTCCC 
ATTCTGCGGC CCAACCGCAG CCATCCCACC CGAATCTCTC TGGTCTTCTA CCAGCACAAG 
AGCCTTAACG TGCCTGGTCA CGGGCTGCTC CGGTGGGAAG CCAAGATGGC GGAGAAAGCC 
AGGGAGAAAG AGGAGGAGGC TATGCGGCTG GGACTAGAGA CGGAAGGTCT TGGGTCAGCT 
AAGACCAGAA AATCACAGCC GGCAGCGGGG AGCGACACGG AAAGCGGTGA GGAGGATGTG 
TGGCAGGACG AGAGGGAGAA GCTACAGGTC CCTACCCGTC AGTCACTAAC AGCTACTCGG 
GACGGCCTCG TCACATCAGC CCCCTACTCC CTTACCCGGG TCACCGGGCC TTACAACCGC 
TGGATGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_007240336.1
CDS	168..4556
Translation	MLPKIEALPLRSEKVVCKNIPKSRKRNRVSKVDSEGILVNGTNSEQMEETCVVPEEEARDVSQAHSAPPDSPTPLGHDTEPAPAERALQPAVSHHALLTHQSSLSSHNGANHMEPNTEDHRDPTDLQSKSQRTVNMPTKSQTPEAIPAQTVPLKKIKLEEPSTVSDQHSTSYLSSSDFYQDALSTLANVVCSTISDLKGLEEKTVGPQTSNACNIKTEREEEPYQSHSHQERTSNSPAGADNSHPKDPVPLTNNSVQSLVEHRNISIDQAIAIEALTQLAATPKSAPVKADSKVQDPPQESCNKLANVSSSRQSPVQEAKPISEVISNKVSVINSHQTSVICSPLNRQENPTHPKLSLQDLLIASSYDKLLHSPENGRVSQALCKTETLDGTFKTFKHVERTQTSRRRDEEEVAAQLVQLAFIIQSRTNPMSSENSPPKGMPVQTIKYNHHVVGQNVKKQRKPRTTPSSPRISKKKASEVEGGNHRIPLAKRTPNSKPPFKTKAQHVALQEKPKPQPKWSPFLPQTQIDLKKYLAQAHHEKHQLFHFSSKRQELDMQTLKSSGNQKAFTYKTDHAALSHSHVNQFRSNGHCHSLTNGHLGATHGQKYECEEHLISQVSKTYSVLNHGANPQAQYAMPSVPGADPLPGSPGRCHKVNSLSCEPRQTSFGQNGRYKVDTAGAVTVLSVSAGNMENANTESVEENTPTKHMLNSFLESPLKYLDTPTKNLINTPSKKTPELPSCDCMEQIIEKEEGPFYTHLGSGPTVAAVREMMEDRYGEKGKAVRVEVVVYTGREGRSSQGCPIAKWVIRRASEEEKLLCLVRQRAGHRCQSAVVVILILAWEGIPRNMADRLYQELTQTLYKYGSPTSRRCALNEDRTCACQGLDPDTCGASFSFGCSWSMYFNGCKFARSKVPRKFRLQGDYPEEEEKIESNLLNLATDVAPLYKKLAPEAYENQVEQEQSGQNCRLGRREGRPFSGVTACVDFCAHAHRDTHNMHNGSTVVCTLTKEDNRAVRNIPEDEQLHVLPLYKISETDEFGRVEGQWAKIQTGALQVLSSFPREVRLQAEPVKSARKRRQEAKREREKQRNQEKKQVTPVKVKNEPLKGFKNSSSELSPCFKTEACSPTPYCSPMRLPRTPDKGSHLLDSASPYRSTSCTPPLSSTRDTAALSSSPHTVLPVPQYQNVGELEQSNESSLLRQRPGLEAARHSSPELSEQKPFRNQQQMGGSLGFRDKCFAVKPEPEEVRCFQGGAAPRLLSPSRAEGLHSRLNFRQAQPSLDDHHGPPVTPRPLTPEEVKAEEVWSDSEHNFLDGDIGGVAVAPSHGSILIECARRELHATTPILRPNRSHPTRISLVFYQHKSLNVPGHGLLRWEAKMAEKAREKEEEAMRLGLETEGLGSAKTRKSQPAAGSDTESGEEDVWQDEREKLQVPTRQSLTATRDGLVTSAPYSLTRVTGPYNRWM

Target ORF information:

RefSeq Version	XM_007240274.2
Organism	Astyanax mexicanus(Mexican tetra)
Definition	Astyanax mexicanus methylcytosine dioxygenase TET3-like (LOC103033153), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_007240274.2

ORF Insert Sequence:

ATGCTGCCTA AAATTGAGGC TCTGCCTCTG AGGAGCGAGA AGGTGGTGTG CAAGAATATT 
CCCAAATCAC GCAAAAGGAA CAGAGTGTCC AAGGTTGACT CAGAGGGCAT CTTAGTAAAT 
GGCACTAATT CAGAGCAGAT GGAAGAGACC TGTGTGGTGC CAGAGGAAGA GGCGCGTGAC 
GTATCTCAAG CACACTCCGC TCCACCTGAC TCTCCCACGC CTCTCGGGCA CGACACTGAA 
CCAGCACCAG CCGAAAGAGC CCTTCAGCCT GCTGTCAGCC ACCATGCACT TCTTACTCAC 
CAGAGCTCTT TAAGCTCCCA CAATGGTGCT AATCATATGG AACCAAACAC TGAGGATCAC 
AGAGATCCCA CTGACCTGCA GTCTAAGTCT CAAAGGACTG TGAACATGCC CACCAAAAGC 
CAGACGCCTG AGGCAATTCC TGCACAGACA GTTCCTCTGA AGAAGATCAA GCTAGAAGAG 
CCTAGCACAG TGTCAGATCA ACACAGCACA TCCTACCTAA GCAGCAGCGA CTTCTACCAG 
GATGCTTTGT CTACTTTAGC AAATGTTGTA TGTTCAACCA TCTCTGATCT GAAGGGTTTG 
GAGGAGAAGA CAGTTGGTCC TCAGACCTCA AATGCCTGTA ATATTAAAAC TGAAAGAGAA 
GAAGAACCAT ATCAGAGTCA TTCACATCAG GAAAGGACTT CTAACAGTCC AGCAGGTGCT 
GATAACTCCC ATCCGAAAGA CCCAGTTCCG CTGACTAATA ATAGTGTCCA GTCTTTAGTT 
GAGCATAGGA ACATAAGCAT TGATCAGGCA ATAGCTATTG AGGCCTTGAC CCAGCTGGCA 
GCTACCCCAA AATCAGCTCC TGTCAAGGCA GATTCTAAAG TTCAGGACCC TCCACAAGAA 
TCTTGTAACA AATTAGCAAA TGTTTCATCA AGCAGGCAAA GCCCGGTCCA AGAGGCTAAA 
CCCATATCTG AGGTCATCTC CAATAAAGTC TCTGTAATCA ATTCACATCA GACTTCTGTA 
ATTTGCAGCC CTCTGAACAG ACAGGAAAAC CCCACCCACC CCAAACTCTC CTTACAGGAT 
CTTTTAATAG CTAGCTCATA TGATAAGCTC TTGCATAGTC CAGAGAATGG ACGAGTAAGT 
CAAGCACTTT GTAAAACAGA AACGTTAGAT GGCACTTTTA AGACTTTTAA GCATGTTGAA 
AGGACGCAGA CCTCAAGGAG GAGAGATGAG GAGGAAGTTG CAGCTCAGTT GGTGCAACTT 
GCATTCATAA TTCAATCAAG AACCAATCCC ATGTCTTCTG AGAACAGTCC CCCCAAAGGG 
ATGCCAGTAC AGACCATAAA ATACAACCAT CATGTCGTTG GACAAAATGT AAAAAAGCAA 
CGGAAACCAA GAACAACTCC CTCAAGTCCC AGGATATCAA AGAAAAAAGC CTCTGAGGTT 
GAGGGAGGCA ACCATAGGAT ACCACTAGCC AAAAGGACAC CCAATAGCAA ACCCCCTTTC 
AAGACAAAAG CCCAGCATGT GGCCTTGCAG GAAAAGCCCA AACCACAACC CAAGTGGAGC 
CCGTTTCTCC CACAGACTCA GATAGACCTG AAAAAATACC TAGCACAGGC TCATCATGAG 
AAACATCAGC TGTTCCATTT TAGCAGCAAG AGACAAGAGT TGGACATGCA GACTCTCAAG 
AGTTCCGGAA ATCAGAAAGC TTTTACTTAC AAAACAGACC ACGCAGCTCT GAGTCATTCC 
CATGTTAACC AATTCAGATC CAACGGACAT TGTCACAGTC TCACAAATGG TCATCTAGGT 
GCTACACATG GCCAGAAGTA TGAATGTGAA GAACATTTGA TTTCTCAGGT ATCGAAAACA 
TACAGTGTGC TAAATCATGG TGCTAATCCA CAAGCCCAGT ATGCCATGCC TAGTGTCCCT 
GGTGCAGATC CATTGCCAGG GAGTCCAGGG AGGTGCCATA AAGTAAACAG CCTAAGCTGT 
GAGCCGCGGC AGACTTCCTT TGGTCAGAAT GGACGTTACA AAGTGGACAC GGCTGGCGCT 
GTCACCGTTT TGTCTGTGTC AGCTGGAAAC ATGGAAAATG CTAACACTGA ATCCGTTGAA 
GAAAACACTC CCACCAAGCA TATGCTTAAC AGCTTTTTGG AATCTCCGTT GAAGTACTTG 
GACACTCCAA CCAAGAATCT AATCAACACA CCCTCCAAAA AAACTCCGGA GCTCCCCTCC 
TGTGACTGTA TGGAGCAAAT CATTGAAAAA GAGGAGGGTC CATTCTACAC ACACCTTGGC 
TCTGGGCCAA CTGTAGCAGC AGTCCGAGAG ATGATGGAGG ACAGGTATGG GGAGAAGGGC 
AAAGCAGTGC GTGTGGAAGT TGTAGTTTAT ACTGGGAGAG AAGGAAGGAG CTCCCAGGGC 
TGTCCAATAG CCAAATGGGT GATCCGTCGG GCCAGTGAGG AAGAGAAGCT GCTGTGCCTA 
GTGCGCCAGC GTGCAGGTCA CCGCTGTCAG AGCGCAGTAG TGGTAATCCT CATCCTGGCC 
TGGGAGGGGA TTCCACGCAA CATGGCCGAC AGACTGTACC AGGAGCTTAC CCAGACCCTC 
TATAAATATG GCTCGCCCAC CAGCCGCCGC TGCGCCCTTA ATGAAGATCG TACCTGTGCC 
TGTCAGGGGC TGGACCCAGA TACCTGTGGA GCGTCCTTCT CTTTCGGCTG CTCGTGGAGT 
ATGTATTTTA ACGGCTGCAA GTTTGCCCGG AGCAAAGTGC CTAGAAAGTT CCGGCTGCAA 
GGAGACTACC CAGAGGAGGA AGAAAAGATC GAAAGCAACC TCCTAAATCT GGCAACTGAT 
GTTGCACCTT TGTATAAGAA ACTTGCTCCT GAAGCCTATG AAAACCAGGT GGAGCAGGAG 
CAGTCAGGGC AAAACTGTCG GCTGGGCAGG AGAGAGGGTC GGCCATTTTC TGGTGTGACA 
GCATGTGTGG ACTTTTGTGC TCATGCTCAC AGGGACACAC ACAACATGCA CAACGGGAGT 
ACTGTGGTAT GCACTTTAAC TAAGGAGGAT AACCGTGCTG TAAGAAACAT TCCAGAGGAT 
GAGCAGCTGC ATGTCCTGCC TCTTTACAAG ATCTCGGAGA CTGATGAGTT CGGTCGTGTG 
GAGGGTCAGT GGGCTAAGAT ACAGACTGGA GCTCTGCAGG TCCTCTCTTC CTTCCCAAGA 
GAGGTGCGTC TGCAGGCAGA GCCCGTCAAG TCTGCCCGTA AAAGGAGACA AGAGGCCAAG 
AGAGAGCGAG AGAAGCAGAG AAACCAGGAG AAGAAGCAGG TCACACCAGT GAAAGTGAAG 
AACGAGCCCC TCAAAGGTTT TAAAAACTCT TCCTCTGAGC TTTCACCGTG TTTCAAAACA 
GAAGCATGTT CTCCAACTCC TTATTGTTCC CCAATGCGAC TACCTAGGAC TCCAGACAAG 
GGGAGCCACT TACTGGACTC GGCCAGTCCT TACAGGAGCA CATCATGTAC TCCTCCATTA 
AGCTCTACCA GAGACACAGC AGCACTTTCG TCCTCCCCTC ACACCGTCCT CCCTGTGCCA 
CAATATCAGA ATGTTGGAGA ACTGGAGCAG AGCAATGAAT CTTCCCTCCT CAGACAGAGA 
CCAGGGTTAG AAGCAGCAAG ACACAGCTCC CCTGAGCTCT CAGAACAGAA ACCATTCAGA 
AACCAGCAGC AGATGGGCGG ATCTTTGGGT TTCCGTGACA AATGTTTTGC AGTAAAACCG 
GAGCCTGAGG AGGTCCGTTG TTTTCAAGGA GGAGCTGCTC CCCGACTGCT CTCCCCTTCC 
CGGGCTGAGG GTCTCCACAG CCGCCTGAAC TTTCGCCAAG CACAGCCGAG CCTGGATGAC 
CACCACGGGC CGCCGGTCAC CCCAAGGCCC CTGACCCCTG AGGAGGTCAA GGCAGAAGAG 
GTGTGGTCAG ACAGCGAGCA CAACTTCCTA GATGGAGACA TAGGCGGGGT CGCGGTGGCC 
CCCTCTCACG GCTCCATCCT TATCGAGTGT GCCCGGCGGG AGCTGCACGC TACCACTCCC 
ATTCTGCGGC CCAACCGCAG CCATCCCACC CGAATCTCTC TGGTCTTCTA CCAGCACAAG 
AGCCTTAACG TGCCTGGTCA CGGGCTGCTC CGGTGGGAAG CCAAGATGGC GGAGAAAGCC 
AGGGAGAAAG AGGAGGAGGC TATGCGGCTG GGACTAGAGA CGGAAGGTCT TGGGTCAGCT 
AAGACCAGAA AATCACAGCC GGCAGCGGGG AGCGACACGG AAAGCGGTGA GGAGGATGTG 
TGGCAGGACG AGAGGGAGAA GCTACAGGTC CCTACCCGTC AGTCACTAAC AGCTACTCGG 
GACGGCCTCG TCACATCAGC CCCCTACTCC CTTACCCGGG TCACCGGGCC TTACAACCGC 
TGGATGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC103033153 ( XM_007240272.3 ) cDNA ORF clone, Astyanax mexicanus(Mexican tetra) -> XP_007240334.2 Astyanax mexicanus tet methylcytosine dioxygenase 1 (tet1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OAs120915
Clone ID Related Accession (Same CDS sequence)	XM_007240272.3
Accession Version	XM_007240272.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5811bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1506873600000
Organism	Astyanax mexicanus(Mexican tetra)
Product	methylcytosine dioxygenase TET1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_035921.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Oct 3, 2017 this sequence version replaced XM_007240272.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Astyanax mexicanus Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCTCGTC CTCCCAAACC ACAAAGGAAG GTGCATCCCC CAGCTAAGCG AAATGTGAGG 
AAGTTGAGGG CGTCCACTGA TAAAAAGCGA GTAGTAAATT CAAACAAGCC CAAGGGTCCA 
GCAACACGTA CAAGACAGGC GAAGAGCAGG AAATCCACAG CCAAGAAACA GGCAACCAAG 
AAGCTCCAGG CAGGTGCTGC TGATAGCAAG TCTGCCGTAA AGGCATCCCA GGAAAGAGCA 
AGCAGAAGCA AGAACACATC TAACTCAACA GAATCTGTGA GAATCTCCAG ACACACGTGT 
AACTCTCTGT CACAGCATGA CCCGGCTGCA GACCTCAGGA GCAGAACGAG GTCTTTTCGA 
GGCAGCCCGT TGTTCCAGAC TTTCCCACAG GAAGTAAAGC CAACAAGGAG GGCGCCCTCA 
AGCAGAGGGA GAGGTCAGCA GGCAAGGGAA CAGGATAATT TCAAAATCAC AGAATGTAAT 
GACCCTAATA GTGATGATCA ACAAAAGAAA GCACTTGTTA CCAATGGAGA GGCTCAACTT 
GTTAACAGAA ACTCTGATGA AACAGCAGAG GAGGAGCACA GAGAACCTCT TGTTCACGAG 
ACTCCTGCGG TGGTGGATGA GGAGGAGGTG GCTGTGGCTG CGGTCACTGC AGAAACAGTG 
CAAAGTGAAA ACTGCCTAGC CTTCATTGAT AACACTGAAC AACCAAGCAC AACTAATGAG 
TCAGATGACG CATTTGTGAA AGAGCATGAC CCAGCTGTTA AAGTGGACTA CGTTTGTGAA 
ACTGATACCT CAGGCCCAAC AGATCCTCCT CCTTCAGACC TTCAGTCTTG TGACGTGGCT 
ATTCGAAGCA CTGTAGACGA TTCTTCTCAA CCAGATGTGT CTGTGGCATC TCCCGTATGT 
GAAATTCCCA AAGAATCACC AGCACTTCTG GAAGCTACAG ATGTGAAGTA TGCTGAGAAC 
GGAGAGCTGG ACAGCACTGA CGACTTAAAG ACCAAAGATG AGAATTATGA CAATGACAAT 
GATGAGTTTG CAAGCAAGAA CGAAGGAGCA CTTTCACTCC TCTCACTGAG CACTGGTGCT 
TATAGTGGCT TTCTGTCCAC TCTCGACTGT GGGCCTTCAA CAACAGCATC ACTTGACAGC 
AGCAAAGTGT CCAGCAGCAC AGATAGCACC CGATTTTCAT TTGATACCGA GTCTGAAGCA 
GGGAGTTTAG AGCTAGCTCT AGAGCACAGA GCTGCTGGTG CTTCTCCTCT GCTGGATGTG 
GATATAAGGC AGCGCTTACT GCAAGCTCAG GTGAGAAAAG AGAGGAAGAA GAGGAGTAGA 
TGTGGGGCCT GCGGACCCTG CCTCCTCAAG TACAGCTGTG GGCAGTGCAG CTGCTGCCTA 
AATCGAAAGA CTGGCCATCA GATCTGTAAG CTCAGAAAAT GTGTGATGCT GAAGAAAAGA 
CCCTGGCAAG TTACAGCTGG AGAGGTGGTG TGCAAGAATA TTCCCAAATC ACGCAAAAGG 
AACAGAGTGT CCAAGGTTGA CTCAGAGGGC ATCTTAGTAA ATGGCACTAA TTCAGAGCAG 
ATGGAAGAGA CCTGTGTGGT GCCAGAGGAA GAGGCGCGTG ACGTATCTCA AGCACACTCC 
GCTCCACCTG ACTCTCCCAC GCCTCTCGGG CACGACACTG AACCAGCACC AGCCGAAAGA 
GCCCTTCAGC CTGCTGTCAG CCACCATGCA CTTCTTACTC ACCAGAGCTC TTTAAGCTCC 
CACAATGGTG CTAATCATAT GGAACCAAAC ACTGAGGATC ACAGAGATCC CACTGACCTG 
CAGTCTAAGT CTCAAAGGAC TGTGAACATG CCCACCAAAA GCCAGACACC TGAGGCAATT 
CCTGCACAGA CAGTTCCTCT GAAGAAGATC AAGCTAGAAG AGCCTAGCAC AGTGTCAGAT 
CAACACAGCA CATCCTACCT AAGCAGCAGC GACTTCTACC AGGATGCTTT GTCTACTTTA 
GCAAATGTTG TATGTTCAAC CATCTCTGAT CTGAAGGGTT TGGAGGAGAA GACAGTTGGT 
CCTCAGACCT CAAATGCCTG TAATATTAAA ACTGAAAGAG AAGAAGAACC ATATCAGAGT 
CATTCACATC AGGAAAGGAC TTCTAACAGT CCAGCAGGTG CTGATAACTC CCATCCGAAA 
GACCCAGTTC CGCTGACTAA TAATAGTGTC CAGTCTTTAG TTGAGCATAG GAACATAAGC 
ATTGATCAGG CAATAGCTAT TGAGGCCTTG ACCCAGCTGG CAGCTACCCC AAAATCAGCT 
CCTGTCAAGG CAGATTCTAA AGTTCAGGAC CCTCCACAAG AATCTTGTAA CAAATTAGCA 
AATGTTTCAT CAAGCAGGCA AAGTCCGGTC CAAGAGGCTA AACCCATATC TGAGGTCATC 
TCCAATAAAG TCTCTGTAAT CAATTCACAT CAGACTTCTG TAATTTGCAG CCCTCTGAAC 
AGACAGGAAA ACCCCACCCA CCCCAAACTC TCCTTACAGG ATCTTTTAAT AGCTAGCTCA 
TATGATAAGC TCTTGCATAG TCCAGAGAAT GGACGAGTAA GTCAAGCACT TTGTAAAACA 
GAAAAGTTAG ATGGCACTTT TAAGACTTTT AAGCATGTTG AAAGGACGCA GACCTCAAGG 
AGGAGAGATG AGGAGGAAGT TGCAGCTCAG TTGGTGCAAC TTGCATTCAT AATTCAATCA 
AGAACCAATC CCATGTCTTC TGAGAACAGT CCCCCCAAAG GGATGCCAGT ACAGACCATA 
AAATACAACC ATCATGTCGT TGGACAAAAT GTAAAAAAGC AACGGAAACC AAGAACAACT 
CCCTCAAGTC CCAGGATATC AAAGAAAAAA GCCTCTGAGG TTGAGGGAGG CAACCATAGG 
ATACCACTAG CCAAAAGGAC ACCCAATAGC AAACCCCCTT TCAAGACAAA AGCCCAGCAT 
GTGGCCTTGC AGGAAAAGCC CAAACCACAA CCCAAGTGGA GCCCGTTTCT CCCACAGACT 
CAGATAGACC TGAAAAAATA CCTAGCACAG GCTCATCATG AGAAACATCA GCTGTTCCAT 
TTTAGCAGCA AGAGACAAGA CTTGGACATG CAGACTCTCA AGAGTTCCGG AAATCAGAAA 
GCTTTTACTT ACAAAACAGA CCACGCAGCT CTGAGTCATT CCCATGTTAA CCAATTCAGA 
TCCAACGGAC ATTGTCACAG TCTCACAAAT GGTCATCTAG GTGCTACACA TGGCCAGAAG 
TATGAATGTG AAGAACATTT GATTTCTCAG GTATCGAAAA CATACAGTGT GCTAAATCAT 
GGTGCTAATC CACAAGCCCA GTATGCCATG CCTAGTGTCC CTGGTGCAGA TCCATTGCCA 
GGGAGTCCAG GGAGGTGCCA TAAAGTAAAC AGCCTAAGCT GTGAGCCGCG GCAGACTTCC 
TTTGGTCAGA ATGGACGTTA CAAAGTGGAC ACGGCTGGCG CTGTCACCGT TTTGTCTGTG 
TCAGCTGGAA ACATGGAAAA TGCTAACACT GAATCCGTTG AAGAAAACAC TCCCACCAAG 
CATATGCTTA ACAGCTTTTT GGAATCTCCG TTGAAGTACT TGGACACTCC AACCAAGAAT 
CTAATCAACA CACCCTCCAA AAAAACTCCG GAGCTCCCCT CCTGTGACTG TATGGAGCAA 
ATCATTGAAA AAGAGGAGGG TCCATTCTAC ACACACCTTG GCTCTGGGCC AACTGTAGCA 
GCAGTCCGAG AGATGATGGA GGACAGGTAT GGGGAGAAGG GCAAAGCAGT GCGTGTGGAA 
GTTGTAGTTT ATACTGGGAG AGAAGGAAGG AGCTCCCAGG GCTGTCCAAT AGCCAAATGG 
GTGATCCGTC GGGCCAGTGA GGAAGAGAAG CTGCTGTGCC TAGTGCGCCA GCGTGCAGGT 
CACCGCTGTC AGAGCGCAGT AGTGGTAATC CTCATCCTGG CCTGGGAGGG GATTCCACGC 
AACATGGCCG ACAGACTGTA CCAGGAGCTT ACCCAGACCC TCTATAAATA TGGCTCGCCC 
ACCAGCCGCC GCTGCGCCCT TAATGAAGAT CGTACCTGTG CCTGTCAGGG GCTGGACCCA 
GATACCTGTG GAGCGTCCTT CTCTTTCGGC TGCTCGTGGA GTATGTATTT TAACGGCTGC 
AAGTTTGCCC GGAGCAAAGT GCCTAGAAAG TTCCGGCTGC AAGGAGACTA CCCAGAGGAG 
GAAGAAAAGA TCGAAAGCAA CCTCCTAAAT CTGGCAACTG ATGTTGCACC TTTGTATAAG 
AAACTTGCTC CTGAAGCCTA TGAAAACCAG GTGGAGCAGG AGCAGTCAGG GCAAAACTGT 
CGGCTGGGCA GGAGAGAGGG TCGGCCATTT TCTGGTGTGA CAGCATGTGT GGACTTTTGT 
GCTCATGCTC ACAGGGACAC ACACAACATG CACAACGGGA GTACTGTGGT ATGCACTTTA 
ACTAAGGAGG ATAACCGTGC TGTAAGAAAC ATTCCAGAGG ATGAGCAGCT GCATGTCCTG 
CCTCTTTACA AGATCTCGGA GACTGATGAG TTCGGTCGTG TGGAGGGTCA GTGGGCTAAG 
ATACAGACTG GAGCTCTGCA GGTCCTCTCT TCCTTCCCAA GAGAGGTGCG TCTGCAGGCA 
GAGCCCGTCA AGTCTGCCCG TAAAAGGAGA CAAGAGGCCA AGAGAGAGCG AGAGAAGCAG 
AGAAACCAGG AGAAGAAGCA GGTCACACCA GTGAAAGTGA AGAACGAGCC CCTCAAAGGT 
TTTAAAAACT CTTCCTCTGA GCTTTCACCG TGTTTCAAAA CAGAAGCATG TTCTCCAACT 
CCTTATTGTT CCCCAATGCG ACTACCTAGG ACTCCAGACA AGGGGAGCCA CTTACTGGAC 
TCGGCCAGTC CTTACAGGAG CACATCATGT ACTCCTCCAT TAAGCTCTAC CAGAGACACA 
GCAGCACTTT CGTCCTCCCC TCACACCGTC CTCCCTGTGC CACAATATCA GAATGTTGGA 
GAACTGGAGC AGAGCAATGA ATCTTCCCTC CTCAGACAGA GACCAGGGTT AGAAGCAGCA 
AGACACAGCT CCCCTGAGCT CTCAGAACAG AAACCATTCA GAAACCAGCA GCAGATGGGC 
GGATCTTTGG GTTTCCGTGA CAAATGTTTT GCAGTAAAAC CGGAGCCTGA GGAGGTCCGT 
TGTTTTCAAG GAGGAGCTGC TCCCCGACTG CTCTCCCCTT CCCGGGCTGA GGGTCTCCAC 
AGCCGCCTGA ACTTTCGCCA AGCACAGCCG AGCCTGGATG ACCACCACGG GCCGCCGGTC 
ACCCCAAGGC CCCTGACCCC TGAGGAGGTC AAGGCAGAAG AGGTGTGGTC AGACAGCGAG 
CACAACTTCC TAGATGGAGA CATAGGCGGG GTCGCGGTGG CCCCCTCTCA CGGCTCCATC 
CTTATCGAGT GTGCCCGGCG GGAGCTGCAC GCTACCACTC CCATTCTGCG GCCCAACCGC 
AGCCATCCCA CCCGAATCTC TCTGGTCTTC TACCAGCACA AGAGCCTTAA CGTGCCTGGT 
CACGGGCTGC TCCGGTGGGA AGCCAAGATG GCGGAGAAAG CCAGGGAGAA AGAGGAGGAG 
GCTATGCGGC TGGGACTAGA GACGGAAGGT CTTGGGTCAG CTAAGACCAG AAAATCACAG 
CCGGCAGCGG GGAGCGACAC GGAAAGTGGT GAGGAGGATG TGTGGCAGGA CGAGAGGGAG 
AAGCTACAGG TCCCTACCCG TCAGTCACTA ACAGCTACTC GGGACGGCCT CGTCACATCA 
GCCCCCTACT CCCTTACCCG GGTCACCGGG CCTTACAACC GCTGGATGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_007240334.2
CDS	472..6282
Translation	MPRPPKPQRKVHPPAKRNVRKLRASTDKKRVVNSNKPKGPATRTRQAKSRKSTAKKQATKKLQAGAADSKSAVKASQERASRSKNTSNSTESVRISRHTCNSLSQHDPAADLRSRTRSFRGSPLFQTFPQEVKPTRRAPSSRGRGQQAREQDNFKITECNDPNSDDQQKKALVTNGEAQLVNRNSDETAEEEHREPLVHETPAVVDEEEVAVAAVTAETVQSENCLAFIDNTEQPSTTNESDDAFVKEHDPAVKVDYVCETDTSGPTDPPPSDLQSCDVAIRSTVDDSSQPDVSVASPVCEIPKESPALLEATDVKYAENGELDSTDDLKTKDENYDNDNDEFASKNEGALSLLSLSTGAYSGFLSTLDCGPSTTASLDSSKVSSSTDSTRFSFDTESEAGSLELALEHRAAGASPLLDVDIRQRLLQAQVRKERKKRSRCGACGPCLLKYSCGQCSCCLNRKTGHQICKLRKCVMLKKRPWQVTAGEVVCKNIPKSRKRNRVSKVDSEGILVNGTNSEQMEETCVVPEEEARDVSQAHSAPPDSPTPLGHDTEPAPAERALQPAVSHHALLTHQSSLSSHNGANHMEPNTEDHRDPTDLQSKSQRTVNMPTKSQTPEAIPAQTVPLKKIKLEEPSTVSDQHSTSYLSSSDFYQDALSTLANVVCSTISDLKGLEEKTVGPQTSNACNIKTEREEEPYQSHSHQERTSNSPAGADNSHPKDPVPLTNNSVQSLVEHRNISIDQAIAIEALTQLAATPKSAPVKADSKVQDPPQESCNKLANVSSSRQSPVQEAKPISEVISNKVSVINSHQTSVICSPLNRQENPTHPKLSLQDLLIASSYDKLLHSPENGRVSQALCKTEKLDGTFKTFKHVERTQTSRRRDEEEVAAQLVQLAFIIQSRTNPMSSENSPPKGMPVQTIKYNHHVVGQNVKKQRKPRTTPSSPRISKKKASEVEGGNHRIPLAKRTPNSKPPFKTKAQHVALQEKPKPQPKWSPFLPQTQIDLKKYLAQAHHEKHQLFHFSSKRQDLDMQTLKSSGNQKAFTYKTDHAALSHSHVNQFRSNGHCHSLTNGHLGATHGQKYECEEHLISQVSKTYSVLNHGANPQAQYAMPSVPGADPLPGSPGRCHKVNSLSCEPRQTSFGQNGRYKVDTAGAVTVLSVSAGNMENANTESVEENTPTKHMLNSFLESPLKYLDTPTKNLINTPSKKTPELPSCDCMEQIIEKEEGPFYTHLGSGPTVAAVREMMEDRYGEKGKAVRVEVVVYTGREGRSSQGCPIAKWVIRRASEEEKLLCLVRQRAGHRCQSAVVVILILAWEGIPRNMADRLYQELTQTLYKYGSPTSRRCALNEDRTCACQGLDPDTCGASFSFGCSWSMYFNGCKFARSKVPRKFRLQGDYPEEEEKIESNLLNLATDVAPLYKKLAPEAYENQVEQEQSGQNCRLGRREGRPFSGVTACVDFCAHAHRDTHNMHNGSTVVCTLTKEDNRAVRNIPEDEQLHVLPLYKISETDEFGRVEGQWAKIQTGALQVLSSFPREVRLQAEPVKSARKRRQEAKREREKQRNQEKKQVTPVKVKNEPLKGFKNSSSELSPCFKTEACSPTPYCSPMRLPRTPDKGSHLLDSASPYRSTSCTPPLSSTRDTAALSSSPHTVLPVPQYQNVGELEQSNESSLLRQRPGLEAARHSSPELSEQKPFRNQQQMGGSLGFRDKCFAVKPEPEEVRCFQGGAAPRLLSPSRAEGLHSRLNFRQAQPSLDDHHGPPVTPRPLTPEEVKAEEVWSDSEHNFLDGDIGGVAVAPSHGSILIECARRELHATTPILRPNRSHPTRISLVFYQHKSLNVPGHGLLRWEAKMAEKAREKEEEAMRLGLETEGLGSAKTRKSQPAAGSDTESGEEDVWQDEREKLQVPTRQSLTATRDGLVTSAPYSLTRVTGPYNRWM

Target ORF information:

RefSeq Version	XM_007240272.3
Organism	Astyanax mexicanus(Mexican tetra)
Definition	Astyanax mexicanus tet methylcytosine dioxygenase 1 (tet1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_007240272.3

ORF Insert Sequence:

ATGCCTCGTC CTCCCAAACC ACAAAGGAAG GTGCATCCCC CAGCTAAGCG AAATGTGAGG 
AAGTTGAGGG CGTCCACTGA TAAAAAGCGA GTAGTAAATT CAAACAAGCC CAAGGGTCCA 
GCAACACGTA CAAGACAGGC GAAGAGCAGG AAATCCACAG CCAAGAAACA GGCAACCAAG 
AAGCTCCAGG CAGGTGCTGC TGATAGCAAG TCTGCCGTAA AGGCATCCCA GGAAAGAGCA 
AGCAGAAGCA AGAACACATC TAACTCAACA GAATCTGTGA GAATCTCCAG ACACACGTGT 
AACTCTCTGT CACAGCATGA CCCGGCTGCA GACCTCAGGA GCAGAACGAG GTCTTTTCGA 
GGCAGCCCGT TGTTCCAGAC TTTCCCACAG GAAGTAAAGC CAACAAGGAG GGCGCCCTCA 
AGCAGAGGGA GAGGTCAGCA GGCAAGGGAA CAGGATAATT TCAAAATCAC AGAATGTAAT 
GACCCTAATA GTGATGATCA ACAAAAGAAA GCACTTGTTA CCAATGGAGA GGCTCAACTT 
GTTAACAGAA ACTCTGATGA AACAGCAGAG GAGGAGCACA GAGAACCTCT TGTTCACGAG 
ACTCCTGCGG TGGTGGATGA GGAGGAGGTG GCTGTGGCTG CGGTCACTGC AGAAACAGTG 
CAAAGTGAAA ACTGCCTAGC CTTCATTGAT AACACTGAAC AACCAAGCAC AACTAATGAG 
TCAGATGACG CATTTGTGAA AGAGCATGAC CCAGCTGTTA AAGTGGACTA CGTTTGTGAA 
ACTGATACCT CAGGCCCAAC AGATCCTCCT CCTTCAGACC TTCAGTCTTG TGACGTGGCT 
ATTCGAAGCA CTGTAGACGA TTCTTCTCAA CCAGATGTGT CTGTGGCATC TCCCGTATGT 
GAAATTCCCA AAGAATCACC AGCACTTCTG GAAGCTACAG ATGTGAAGTA TGCTGAGAAC 
GGAGAGCTGG ACAGCACTGA CGACTTAAAG ACCAAAGATG AGAATTATGA CAATGACAAT 
GATGAGTTTG CAAGCAAGAA CGAAGGAGCA CTTTCACTCC TCTCACTGAG CACTGGTGCT 
TATAGTGGCT TTCTGTCCAC TCTCGACTGT GGGCCTTCAA CAACAGCATC ACTTGACAGC 
AGCAAAGTGT CCAGCAGCAC AGATAGCACC CGATTTTCAT TTGATACCGA GTCTGAAGCA 
GGGAGTTTAG AGCTAGCTCT AGAGCACAGA GCTGCTGGTG CTTCTCCTCT GCTGGATGTG 
GATATAAGGC AGCGCTTACT GCAAGCTCAG GTGAGAAAAG AGAGGAAGAA GAGGAGTAGA 
TGTGGGGCCT GCGGACCCTG CCTCCTCAAG TACAGCTGTG GGCAGTGCAG CTGCTGCCTA 
AATCGAAAGA CTGGCCATCA GATCTGTAAG CTCAGAAAAT GTGTGATGCT GAAGAAAAGA 
CCCTGGCAAG TTACAGCTGG AGAGGTGGTG TGCAAGAATA TTCCCAAATC ACGCAAAAGG 
AACAGAGTGT CCAAGGTTGA CTCAGAGGGC ATCTTAGTAA ATGGCACTAA TTCAGAGCAG 
ATGGAAGAGA CCTGTGTGGT GCCAGAGGAA GAGGCGCGTG ACGTATCTCA AGCACACTCC 
GCTCCACCTG ACTCTCCCAC GCCTCTCGGG CACGACACTG AACCAGCACC AGCCGAAAGA 
GCCCTTCAGC CTGCTGTCAG CCACCATGCA CTTCTTACTC ACCAGAGCTC TTTAAGCTCC 
CACAATGGTG CTAATCATAT GGAACCAAAC ACTGAGGATC ACAGAGATCC CACTGACCTG 
CAGTCTAAGT CTCAAAGGAC TGTGAACATG CCCACCAAAA GCCAGACACC TGAGGCAATT 
CCTGCACAGA CAGTTCCTCT GAAGAAGATC AAGCTAGAAG AGCCTAGCAC AGTGTCAGAT 
CAACACAGCA CATCCTACCT AAGCAGCAGC GACTTCTACC AGGATGCTTT GTCTACTTTA 
GCAAATGTTG TATGTTCAAC CATCTCTGAT CTGAAGGGTT TGGAGGAGAA GACAGTTGGT 
CCTCAGACCT CAAATGCCTG TAATATTAAA ACTGAAAGAG AAGAAGAACC ATATCAGAGT 
CATTCACATC AGGAAAGGAC TTCTAACAGT CCAGCAGGTG CTGATAACTC CCATCCGAAA 
GACCCAGTTC CGCTGACTAA TAATAGTGTC CAGTCTTTAG TTGAGCATAG GAACATAAGC 
ATTGATCAGG CAATAGCTAT TGAGGCCTTG ACCCAGCTGG CAGCTACCCC AAAATCAGCT 
CCTGTCAAGG CAGATTCTAA AGTTCAGGAC CCTCCACAAG AATCTTGTAA CAAATTAGCA 
AATGTTTCAT CAAGCAGGCA AAGTCCGGTC CAAGAGGCTA AACCCATATC TGAGGTCATC 
TCCAATAAAG TCTCTGTAAT CAATTCACAT CAGACTTCTG TAATTTGCAG CCCTCTGAAC 
AGACAGGAAA ACCCCACCCA CCCCAAACTC TCCTTACAGG ATCTTTTAAT AGCTAGCTCA 
TATGATAAGC TCTTGCATAG TCCAGAGAAT GGACGAGTAA GTCAAGCACT TTGTAAAACA 
GAAAAGTTAG ATGGCACTTT TAAGACTTTT AAGCATGTTG AAAGGACGCA GACCTCAAGG 
AGGAGAGATG AGGAGGAAGT TGCAGCTCAG TTGGTGCAAC TTGCATTCAT AATTCAATCA 
AGAACCAATC CCATGTCTTC TGAGAACAGT CCCCCCAAAG GGATGCCAGT ACAGACCATA 
AAATACAACC ATCATGTCGT TGGACAAAAT GTAAAAAAGC AACGGAAACC AAGAACAACT 
CCCTCAAGTC CCAGGATATC AAAGAAAAAA GCCTCTGAGG TTGAGGGAGG CAACCATAGG 
ATACCACTAG CCAAAAGGAC ACCCAATAGC AAACCCCCTT TCAAGACAAA AGCCCAGCAT 
GTGGCCTTGC AGGAAAAGCC CAAACCACAA CCCAAGTGGA GCCCGTTTCT CCCACAGACT 
CAGATAGACC TGAAAAAATA CCTAGCACAG GCTCATCATG AGAAACATCA GCTGTTCCAT 
TTTAGCAGCA AGAGACAAGA CTTGGACATG CAGACTCTCA AGAGTTCCGG AAATCAGAAA 
GCTTTTACTT ACAAAACAGA CCACGCAGCT CTGAGTCATT CCCATGTTAA CCAATTCAGA 
TCCAACGGAC ATTGTCACAG TCTCACAAAT GGTCATCTAG GTGCTACACA TGGCCAGAAG 
TATGAATGTG AAGAACATTT GATTTCTCAG GTATCGAAAA CATACAGTGT GCTAAATCAT 
GGTGCTAATC CACAAGCCCA GTATGCCATG CCTAGTGTCC CTGGTGCAGA TCCATTGCCA 
GGGAGTCCAG GGAGGTGCCA TAAAGTAAAC AGCCTAAGCT GTGAGCCGCG GCAGACTTCC 
TTTGGTCAGA ATGGACGTTA CAAAGTGGAC ACGGCTGGCG CTGTCACCGT TTTGTCTGTG 
TCAGCTGGAA ACATGGAAAA TGCTAACACT GAATCCGTTG AAGAAAACAC TCCCACCAAG 
CATATGCTTA ACAGCTTTTT GGAATCTCCG TTGAAGTACT TGGACACTCC AACCAAGAAT 
CTAATCAACA CACCCTCCAA AAAAACTCCG GAGCTCCCCT CCTGTGACTG TATGGAGCAA 
ATCATTGAAA AAGAGGAGGG TCCATTCTAC ACACACCTTG GCTCTGGGCC AACTGTAGCA 
GCAGTCCGAG AGATGATGGA GGACAGGTAT GGGGAGAAGG GCAAAGCAGT GCGTGTGGAA 
GTTGTAGTTT ATACTGGGAG AGAAGGAAGG AGCTCCCAGG GCTGTCCAAT AGCCAAATGG 
GTGATCCGTC GGGCCAGTGA GGAAGAGAAG CTGCTGTGCC TAGTGCGCCA GCGTGCAGGT 
CACCGCTGTC AGAGCGCAGT AGTGGTAATC CTCATCCTGG CCTGGGAGGG GATTCCACGC 
AACATGGCCG ACAGACTGTA CCAGGAGCTT ACCCAGACCC TCTATAAATA TGGCTCGCCC 
ACCAGCCGCC GCTGCGCCCT TAATGAAGAT CGTACCTGTG CCTGTCAGGG GCTGGACCCA 
GATACCTGTG GAGCGTCCTT CTCTTTCGGC TGCTCGTGGA GTATGTATTT TAACGGCTGC 
AAGTTTGCCC GGAGCAAAGT GCCTAGAAAG TTCCGGCTGC AAGGAGACTA CCCAGAGGAG 
GAAGAAAAGA TCGAAAGCAA CCTCCTAAAT CTGGCAACTG ATGTTGCACC TTTGTATAAG 
AAACTTGCTC CTGAAGCCTA TGAAAACCAG GTGGAGCAGG AGCAGTCAGG GCAAAACTGT 
CGGCTGGGCA GGAGAGAGGG TCGGCCATTT TCTGGTGTGA CAGCATGTGT GGACTTTTGT 
GCTCATGCTC ACAGGGACAC ACACAACATG CACAACGGGA GTACTGTGGT ATGCACTTTA 
ACTAAGGAGG ATAACCGTGC TGTAAGAAAC ATTCCAGAGG ATGAGCAGCT GCATGTCCTG 
CCTCTTTACA AGATCTCGGA GACTGATGAG TTCGGTCGTG TGGAGGGTCA GTGGGCTAAG 
ATACAGACTG GAGCTCTGCA GGTCCTCTCT TCCTTCCCAA GAGAGGTGCG TCTGCAGGCA 
GAGCCCGTCA AGTCTGCCCG TAAAAGGAGA CAAGAGGCCA AGAGAGAGCG AGAGAAGCAG 
AGAAACCAGG AGAAGAAGCA GGTCACACCA GTGAAAGTGA AGAACGAGCC CCTCAAAGGT 
TTTAAAAACT CTTCCTCTGA GCTTTCACCG TGTTTCAAAA CAGAAGCATG TTCTCCAACT 
CCTTATTGTT CCCCAATGCG ACTACCTAGG ACTCCAGACA AGGGGAGCCA CTTACTGGAC 
TCGGCCAGTC CTTACAGGAG CACATCATGT ACTCCTCCAT TAAGCTCTAC CAGAGACACA 
GCAGCACTTT CGTCCTCCCC TCACACCGTC CTCCCTGTGC CACAATATCA GAATGTTGGA 
GAACTGGAGC AGAGCAATGA ATCTTCCCTC CTCAGACAGA GACCAGGGTT AGAAGCAGCA 
AGACACAGCT CCCCTGAGCT CTCAGAACAG AAACCATTCA GAAACCAGCA GCAGATGGGC 
GGATCTTTGG GTTTCCGTGA CAAATGTTTT GCAGTAAAAC CGGAGCCTGA GGAGGTCCGT 
TGTTTTCAAG GAGGAGCTGC TCCCCGACTG CTCTCCCCTT CCCGGGCTGA GGGTCTCCAC 
AGCCGCCTGA ACTTTCGCCA AGCACAGCCG AGCCTGGATG ACCACCACGG GCCGCCGGTC 
ACCCCAAGGC CCCTGACCCC TGAGGAGGTC AAGGCAGAAG AGGTGTGGTC AGACAGCGAG 
CACAACTTCC TAGATGGAGA CATAGGCGGG GTCGCGGTGG CCCCCTCTCA CGGCTCCATC 
CTTATCGAGT GTGCCCGGCG GGAGCTGCAC GCTACCACTC CCATTCTGCG GCCCAACCGC 
AGCCATCCCA CCCGAATCTC TCTGGTCTTC TACCAGCACA AGAGCCTTAA CGTGCCTGGT 
CACGGGCTGC TCCGGTGGGA AGCCAAGATG GCGGAGAAAG CCAGGGAGAA AGAGGAGGAG 
GCTATGCGGC TGGGACTAGA GACGGAAGGT CTTGGGTCAG CTAAGACCAG AAAATCACAG 
CCGGCAGCGG GGAGCGACAC GGAAAGTGGT GAGGAGGATG TGTGGCAGGA CGAGAGGGAG 
AAGCTACAGG TCCCTACCCG TCAGTCACTA ACAGCTACTC GGGACGGCCT CGTCACATCA 
GCCCCCTACT CCCTTACCCG GGTCACCGGG CCTTACAACC GCTGGATGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC103033153 ( XM_007240273.3 ) cDNA ORF clone, Astyanax mexicanus(Mexican tetra) -> XP_007240335.2 Astyanax mexicanus tet methylcytosine dioxygenase 1 (tet1), transcript variant X2, mRNA.

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CloneID	OAs120916
Clone ID Related Accession (Same CDS sequence)	XM_007240273.3
Accession Version	XM_007240273.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5760bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1506873600000
Organism	Astyanax mexicanus(Mexican tetra)
Product	methylcytosine dioxygenase TET1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_035921.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Oct 3, 2017 this sequence version replaced XM_007240273.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Astyanax mexicanus Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCTCGTC CTCCCAAACC ACAAAGGAAG GTGCATCCCC CAGCTAAGCG AAATGTGAGG 
AAGTTGAGGG CGTCCACTGA TAAAAAGCGA GTAGTAAATT CAAACAAGCC CAAGGGTCCA 
GCAACACGTA CAAGACAGGC GAAGAGCAGG AAATCCACAG CCAAGAAACA GGCAACCAAG 
AAGCTCCAGG CAGGTGCTGC TGATAGCAAG TCTGCCGTAA AGGCATCCCA GGAAAGAGCA 
AGCAGAAGCA AGAACACATC TAACTCAACA GAATCTGTGA GAATCTCCAG ACACACGTGT 
AACTCTCTGT CACAGCATGA CCCGGCTGCA GACCTCAGGA GCAGAACGAG GTCTTTTCGA 
GGCAGCCCGT TGTTCCAGAC TTTCCCACAG GAAGTAAAGC CAACAAGGAG GGCGCCCTCA 
AGCAGAGGGA GAGGTCAGCA GGCAAGGGAA CAGGATAATT TCAAAATCAC AGAATGTAAT 
GACCCTAATA GTGATGATCA ACAAAAGAAA GCACTTGTTA CCAATGGAGA GGCTCAACTT 
GTTAACAGAA ACTCTGATGA AACAGCAGAG GAGGAGCACA GAGAACCTCT TGTTCACGAG 
ACTCCTGCGG TGGTGGATGA GGAGGAGGTG GCTGTGGCTG CGGTCACTGC AGAAACAGTG 
CAAAGTGAAA ACTGCCTAGC CTTCATTGAT AACACTGAAC AACCAAGCAC AACTAATGAG 
TCAGATGACG CATTTGTGAA AGAGCATGAC CCAGCTGTTA AAGTGGACTA CGTTTGTGAA 
ACTGATACCT CAGGCCCAAC AGATCCTCCT CCTTCAGACC TTCAGTCTTG TGACGTGGCT 
ATTCGAAGCA CTGTAGACGA TTCTTCTCAA CCAGATGTGT CTGTGGCATC TCCCGTATGT 
GAAATTCCCA AAGAATCACC AGCACTTCTG GAAGCTACAG ATGTGAAGTA TGCTGAGAAC 
GGAGAGCTGG ACAGCACTGA CGACTTAAAG ACCAAAGATG AGAATTATGA CAATGACAAT 
GATGAGTTTG CAAGCAAGAA CGAAGGAGCA CTTTCACTCC TCTCACTGAG CACTGGTGCT 
TATAGTGGCT TTCTGTCCAC TCTCGACTGT GGGCCTTCAA CAACAGCATC ACTTGACAGC 
AGCAAAGTGT CCAGCAGCAC AGATAGCACC CGATTTTCAT TTGATACCGA GTCTGAAGCA 
GGGAGTTTAG AGCTAGCTCT AGAGCACAGA GCTGCTGGTG CTTCTCCTCT GCTGGATGTG 
GATATAAGGC AGCGCTTACT GCAAGCTCAG GTGAGAAAAG AGAGGAAGAA GAGGAGTAGA 
TGTGGGGCCT GCGGACCCTG CCTCCTCAAG TACAGCTGTG GGCAGTGCAG CTGCTGCCTA 
AATCGAAAGA CTGGCCATCA GATCTGTAAG CTCAGAAAAT GTGTGATGCT GAAGAAAAGA 
CCCTGGCAAG TTACAGCTGG AGAGGTTGAC TCAGAGGGCA TCTTAGTAAA TGGCACTAAT 
TCAGAGCAGA TGGAAGAGAC CTGTGTGGTG CCAGAGGAAG AGGCGCGTGA CGTATCTCAA 
GCACACTCCG CTCCACCTGA CTCTCCCACG CCTCTCGGGC ACGACACTGA ACCAGCACCA 
GCCGAAAGAG CCCTTCAGCC TGCTGTCAGC CACCATGCAC TTCTTACTCA CCAGAGCTCT 
TTAAGCTCCC ACAATGGTGC TAATCATATG GAACCAAACA CTGAGGATCA CAGAGATCCC 
ACTGACCTGC AGTCTAAGTC TCAAAGGACT GTGAACATGC CCACCAAAAG CCAGACACCT 
GAGGCAATTC CTGCACAGAC AGTTCCTCTG AAGAAGATCA AGCTAGAAGA GCCTAGCACA 
GTGTCAGATC AACACAGCAC ATCCTACCTA AGCAGCAGCG ACTTCTACCA GGATGCTTTG 
TCTACTTTAG CAAATGTTGT ATGTTCAACC ATCTCTGATC TGAAGGGTTT GGAGGAGAAG 
ACAGTTGGTC CTCAGACCTC AAATGCCTGT AATATTAAAA CTGAAAGAGA AGAAGAACCA 
TATCAGAGTC ATTCACATCA GGAAAGGACT TCTAACAGTC CAGCAGGTGC TGATAACTCC 
CATCCGAAAG ACCCAGTTCC GCTGACTAAT AATAGTGTCC AGTCTTTAGT TGAGCATAGG 
AACATAAGCA TTGATCAGGC AATAGCTATT GAGGCCTTGA CCCAGCTGGC AGCTACCCCA 
AAATCAGCTC CTGTCAAGGC AGATTCTAAA GTTCAGGACC CTCCACAAGA ATCTTGTAAC 
AAATTAGCAA ATGTTTCATC AAGCAGGCAA AGTCCGGTCC AAGAGGCTAA ACCCATATCT 
GAGGTCATCT CCAATAAAGT CTCTGTAATC AATTCACATC AGACTTCTGT AATTTGCAGC 
CCTCTGAACA GACAGGAAAA CCCCACCCAC CCCAAACTCT CCTTACAGGA TCTTTTAATA 
GCTAGCTCAT ATGATAAGCT CTTGCATAGT CCAGAGAATG GACGAGTAAG TCAAGCACTT 
TGTAAAACAG AAAAGTTAGA TGGCACTTTT AAGACTTTTA AGCATGTTGA AAGGACGCAG 
ACCTCAAGGA GGAGAGATGA GGAGGAAGTT GCAGCTCAGT TGGTGCAACT TGCATTCATA 
ATTCAATCAA GAACCAATCC CATGTCTTCT GAGAACAGTC CCCCCAAAGG GATGCCAGTA 
CAGACCATAA AATACAACCA TCATGTCGTT GGACAAAATG TAAAAAAGCA ACGGAAACCA 
AGAACAACTC CCTCAAGTCC CAGGATATCA AAGAAAAAAG CCTCTGAGGT TGAGGGAGGC 
AACCATAGGA TACCACTAGC CAAAAGGACA CCCAATAGCA AACCCCCTTT CAAGACAAAA 
GCCCAGCATG TGGCCTTGCA GGAAAAGCCC AAACCACAAC CCAAGTGGAG CCCGTTTCTC 
CCACAGACTC AGATAGACCT GAAAAAATAC CTAGCACAGG CTCATCATGA GAAACATCAG 
CTGTTCCATT TTAGCAGCAA GAGACAAGAC TTGGACATGC AGACTCTCAA GAGTTCCGGA 
AATCAGAAAG CTTTTACTTA CAAAACAGAC CACGCAGCTC TGAGTCATTC CCATGTTAAC 
CAATTCAGAT CCAACGGACA TTGTCACAGT CTCACAAATG GTCATCTAGG TGCTACACAT 
GGCCAGAAGT ATGAATGTGA AGAACATTTG ATTTCTCAGG TATCGAAAAC ATACAGTGTG 
CTAAATCATG GTGCTAATCC ACAAGCCCAG TATGCCATGC CTAGTGTCCC TGGTGCAGAT 
CCATTGCCAG GGAGTCCAGG GAGGTGCCAT AAAGTAAACA GCCTAAGCTG TGAGCCGCGG 
CAGACTTCCT TTGGTCAGAA TGGACGTTAC AAAGTGGACA CGGCTGGCGC TGTCACCGTT 
TTGTCTGTGT CAGCTGGAAA CATGGAAAAT GCTAACACTG AATCCGTTGA AGAAAACACT 
CCCACCAAGC ATATGCTTAA CAGCTTTTTG GAATCTCCGT TGAAGTACTT GGACACTCCA 
ACCAAGAATC TAATCAACAC ACCCTCCAAA AAAACTCCGG AGCTCCCCTC CTGTGACTGT 
ATGGAGCAAA TCATTGAAAA AGAGGAGGGT CCATTCTACA CACACCTTGG CTCTGGGCCA 
ACTGTAGCAG CAGTCCGAGA GATGATGGAG GACAGGTATG GGGAGAAGGG CAAAGCAGTG 
CGTGTGGAAG TTGTAGTTTA TACTGGGAGA GAAGGAAGGA GCTCCCAGGG CTGTCCAATA 
GCCAAATGGG TGATCCGTCG GGCCAGTGAG GAAGAGAAGC TGCTGTGCCT AGTGCGCCAG 
CGTGCAGGTC ACCGCTGTCA GAGCGCAGTA GTGGTAATCC TCATCCTGGC CTGGGAGGGG 
ATTCCACGCA ACATGGCCGA CAGACTGTAC CAGGAGCTTA CCCAGACCCT CTATAAATAT 
GGCTCGCCCA CCAGCCGCCG CTGCGCCCTT AATGAAGATC GTACCTGTGC CTGTCAGGGG 
CTGGACCCAG ATACCTGTGG AGCGTCCTTC TCTTTCGGCT GCTCGTGGAG TATGTATTTT 
AACGGCTGCA AGTTTGCCCG GAGCAAAGTG CCTAGAAAGT TCCGGCTGCA AGGAGACTAC 
CCAGAGGAGG AAGAAAAGAT CGAAAGCAAC CTCCTAAATC TGGCAACTGA TGTTGCACCT 
TTGTATAAGA AACTTGCTCC TGAAGCCTAT GAAAACCAGG TGGAGCAGGA GCAGTCAGGG 
CAAAACTGTC GGCTGGGCAG GAGAGAGGGT CGGCCATTTT CTGGTGTGAC AGCATGTGTG 
GACTTTTGTG CTCATGCTCA CAGGGACACA CACAACATGC ACAACGGGAG TACTGTGGTA 
TGCACTTTAA CTAAGGAGGA TAACCGTGCT GTAAGAAACA TTCCAGAGGA TGAGCAGCTG 
CATGTCCTGC CTCTTTACAA GATCTCGGAG ACTGATGAGT TCGGTCGTGT GGAGGGTCAG 
TGGGCTAAGA TACAGACTGG AGCTCTGCAG GTCCTCTCTT CCTTCCCAAG AGAGGTGCGT 
CTGCAGGCAG AGCCCGTCAA GTCTGCCCGT AAAAGGAGAC AAGAGGCCAA GAGAGAGCGA 
GAGAAGCAGA GAAACCAGGA GAAGAAGCAG GTCACACCAG TGAAAGTGAA GAACGAGCCC 
CTCAAAGGTT TTAAAAACTC TTCCTCTGAG CTTTCACCGT GTTTCAAAAC AGAAGCATGT 
TCTCCAACTC CTTATTGTTC CCCAATGCGA CTACCTAGGA CTCCAGACAA GGGGAGCCAC 
TTACTGGACT CGGCCAGTCC TTACAGGAGC ACATCATGTA CTCCTCCATT AAGCTCTACC 
AGAGACACAG CAGCACTTTC GTCCTCCCCT CACACCGTCC TCCCTGTGCC ACAATATCAG 
AATGTTGGAG AACTGGAGCA GAGCAATGAA TCTTCCCTCC TCAGACAGAG ACCAGGGTTA 
GAAGCAGCAA GACACAGCTC CCCTGAGCTC TCAGAACAGA AACCATTCAG AAACCAGCAG 
CAGATGGGCG GATCTTTGGG TTTCCGTGAC AAATGTTTTG CAGTAAAACC GGAGCCTGAG 
GAGGTCCGTT GTTTTCAAGG AGGAGCTGCT CCCCGACTGC TCTCCCCTTC CCGGGCTGAG 
GGTCTCCACA GCCGCCTGAA CTTTCGCCAA GCACAGCCGA GCCTGGATGA CCACCACGGG 
CCGCCGGTCA CCCCAAGGCC CCTGACCCCT GAGGAGGTCA AGGCAGAAGA GGTGTGGTCA 
GACAGCGAGC ACAACTTCCT AGATGGAGAC ATAGGCGGGG TCGCGGTGGC CCCCTCTCAC 
GGCTCCATCC TTATCGAGTG TGCCCGGCGG GAGCTGCACG CTACCACTCC CATTCTGCGG 
CCCAACCGCA GCCATCCCAC CCGAATCTCT CTGGTCTTCT ACCAGCACAA GAGCCTTAAC 
GTGCCTGGTC ACGGGCTGCT CCGGTGGGAA GCCAAGATGG CGGAGAAAGC CAGGGAGAAA 
GAGGAGGAGG CTATGCGGCT GGGACTAGAG ACGGAAGGTC TTGGGTCAGC TAAGACCAGA 
AAATCACAGC CGGCAGCGGG GAGCGACACG GAAAGTGGTG AGGAGGATGT GTGGCAGGAC 
GAGAGGGAGA AGCTACAGGT CCCTACCCGT CAGTCACTAA CAGCTACTCG GGACGGCCTC 
GTCACATCAG CCCCCTACTC CCTTACCCGG GTCACCGGGC CTTACAACCG CTGGATGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_007240335.2
CDS	472..6231
Translation	MPRPPKPQRKVHPPAKRNVRKLRASTDKKRVVNSNKPKGPATRTRQAKSRKSTAKKQATKKLQAGAADSKSAVKASQERASRSKNTSNSTESVRISRHTCNSLSQHDPAADLRSRTRSFRGSPLFQTFPQEVKPTRRAPSSRGRGQQAREQDNFKITECNDPNSDDQQKKALVTNGEAQLVNRNSDETAEEEHREPLVHETPAVVDEEEVAVAAVTAETVQSENCLAFIDNTEQPSTTNESDDAFVKEHDPAVKVDYVCETDTSGPTDPPPSDLQSCDVAIRSTVDDSSQPDVSVASPVCEIPKESPALLEATDVKYAENGELDSTDDLKTKDENYDNDNDEFASKNEGALSLLSLSTGAYSGFLSTLDCGPSTTASLDSSKVSSSTDSTRFSFDTESEAGSLELALEHRAAGASPLLDVDIRQRLLQAQVRKERKKRSRCGACGPCLLKYSCGQCSCCLNRKTGHQICKLRKCVMLKKRPWQVTAGEVDSEGILVNGTNSEQMEETCVVPEEEARDVSQAHSAPPDSPTPLGHDTEPAPAERALQPAVSHHALLTHQSSLSSHNGANHMEPNTEDHRDPTDLQSKSQRTVNMPTKSQTPEAIPAQTVPLKKIKLEEPSTVSDQHSTSYLSSSDFYQDALSTLANVVCSTISDLKGLEEKTVGPQTSNACNIKTEREEEPYQSHSHQERTSNSPAGADNSHPKDPVPLTNNSVQSLVEHRNISIDQAIAIEALTQLAATPKSAPVKADSKVQDPPQESCNKLANVSSSRQSPVQEAKPISEVISNKVSVINSHQTSVICSPLNRQENPTHPKLSLQDLLIASSYDKLLHSPENGRVSQALCKTEKLDGTFKTFKHVERTQTSRRRDEEEVAAQLVQLAFIIQSRTNPMSSENSPPKGMPVQTIKYNHHVVGQNVKKQRKPRTTPSSPRISKKKASEVEGGNHRIPLAKRTPNSKPPFKTKAQHVALQEKPKPQPKWSPFLPQTQIDLKKYLAQAHHEKHQLFHFSSKRQDLDMQTLKSSGNQKAFTYKTDHAALSHSHVNQFRSNGHCHSLTNGHLGATHGQKYECEEHLISQVSKTYSVLNHGANPQAQYAMPSVPGADPLPGSPGRCHKVNSLSCEPRQTSFGQNGRYKVDTAGAVTVLSVSAGNMENANTESVEENTPTKHMLNSFLESPLKYLDTPTKNLINTPSKKTPELPSCDCMEQIIEKEEGPFYTHLGSGPTVAAVREMMEDRYGEKGKAVRVEVVVYTGREGRSSQGCPIAKWVIRRASEEEKLLCLVRQRAGHRCQSAVVVILILAWEGIPRNMADRLYQELTQTLYKYGSPTSRRCALNEDRTCACQGLDPDTCGASFSFGCSWSMYFNGCKFARSKVPRKFRLQGDYPEEEEKIESNLLNLATDVAPLYKKLAPEAYENQVEQEQSGQNCRLGRREGRPFSGVTACVDFCAHAHRDTHNMHNGSTVVCTLTKEDNRAVRNIPEDEQLHVLPLYKISETDEFGRVEGQWAKIQTGALQVLSSFPREVRLQAEPVKSARKRRQEAKREREKQRNQEKKQVTPVKVKNEPLKGFKNSSSELSPCFKTEACSPTPYCSPMRLPRTPDKGSHLLDSASPYRSTSCTPPLSSTRDTAALSSSPHTVLPVPQYQNVGELEQSNESSLLRQRPGLEAARHSSPELSEQKPFRNQQQMGGSLGFRDKCFAVKPEPEEVRCFQGGAAPRLLSPSRAEGLHSRLNFRQAQPSLDDHHGPPVTPRPLTPEEVKAEEVWSDSEHNFLDGDIGGVAVAPSHGSILIECARRELHATTPILRPNRSHPTRISLVFYQHKSLNVPGHGLLRWEAKMAEKAREKEEEAMRLGLETEGLGSAKTRKSQPAAGSDTESGEEDVWQDEREKLQVPTRQSLTATRDGLVTSAPYSLTRVTGPYNRWM

Target ORF information:

RefSeq Version	XM_007240273.3
Organism	Astyanax mexicanus(Mexican tetra)
Definition	Astyanax mexicanus tet methylcytosine dioxygenase 1 (tet1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_007240273.3

ORF Insert Sequence:

ATGCCTCGTC CTCCCAAACC ACAAAGGAAG GTGCATCCCC CAGCTAAGCG AAATGTGAGG 
AAGTTGAGGG CGTCCACTGA TAAAAAGCGA GTAGTAAATT CAAACAAGCC CAAGGGTCCA 
GCAACACGTA CAAGACAGGC GAAGAGCAGG AAATCCACAG CCAAGAAACA GGCAACCAAG 
AAGCTCCAGG CAGGTGCTGC TGATAGCAAG TCTGCCGTAA AGGCATCCCA GGAAAGAGCA 
AGCAGAAGCA AGAACACATC TAACTCAACA GAATCTGTGA GAATCTCCAG ACACACGTGT 
AACTCTCTGT CACAGCATGA CCCGGCTGCA GACCTCAGGA GCAGAACGAG GTCTTTTCGA 
GGCAGCCCGT TGTTCCAGAC TTTCCCACAG GAAGTAAAGC CAACAAGGAG GGCGCCCTCA 
AGCAGAGGGA GAGGTCAGCA GGCAAGGGAA CAGGATAATT TCAAAATCAC AGAATGTAAT 
GACCCTAATA GTGATGATCA ACAAAAGAAA GCACTTGTTA CCAATGGAGA GGCTCAACTT 
GTTAACAGAA ACTCTGATGA AACAGCAGAG GAGGAGCACA GAGAACCTCT TGTTCACGAG 
ACTCCTGCGG TGGTGGATGA GGAGGAGGTG GCTGTGGCTG CGGTCACTGC AGAAACAGTG 
CAAAGTGAAA ACTGCCTAGC CTTCATTGAT AACACTGAAC AACCAAGCAC AACTAATGAG 
TCAGATGACG CATTTGTGAA AGAGCATGAC CCAGCTGTTA AAGTGGACTA CGTTTGTGAA 
ACTGATACCT CAGGCCCAAC AGATCCTCCT CCTTCAGACC TTCAGTCTTG TGACGTGGCT 
ATTCGAAGCA CTGTAGACGA TTCTTCTCAA CCAGATGTGT CTGTGGCATC TCCCGTATGT 
GAAATTCCCA AAGAATCACC AGCACTTCTG GAAGCTACAG ATGTGAAGTA TGCTGAGAAC 
GGAGAGCTGG ACAGCACTGA CGACTTAAAG ACCAAAGATG AGAATTATGA CAATGACAAT 
GATGAGTTTG CAAGCAAGAA CGAAGGAGCA CTTTCACTCC TCTCACTGAG CACTGGTGCT 
TATAGTGGCT TTCTGTCCAC TCTCGACTGT GGGCCTTCAA CAACAGCATC ACTTGACAGC 
AGCAAAGTGT CCAGCAGCAC AGATAGCACC CGATTTTCAT TTGATACCGA GTCTGAAGCA 
GGGAGTTTAG AGCTAGCTCT AGAGCACAGA GCTGCTGGTG CTTCTCCTCT GCTGGATGTG 
GATATAAGGC AGCGCTTACT GCAAGCTCAG GTGAGAAAAG AGAGGAAGAA GAGGAGTAGA 
TGTGGGGCCT GCGGACCCTG CCTCCTCAAG TACAGCTGTG GGCAGTGCAG CTGCTGCCTA 
AATCGAAAGA CTGGCCATCA GATCTGTAAG CTCAGAAAAT GTGTGATGCT GAAGAAAAGA 
CCCTGGCAAG TTACAGCTGG AGAGGTTGAC TCAGAGGGCA TCTTAGTAAA TGGCACTAAT 
TCAGAGCAGA TGGAAGAGAC CTGTGTGGTG CCAGAGGAAG AGGCGCGTGA CGTATCTCAA 
GCACACTCCG CTCCACCTGA CTCTCCCACG CCTCTCGGGC ACGACACTGA ACCAGCACCA 
GCCGAAAGAG CCCTTCAGCC TGCTGTCAGC CACCATGCAC TTCTTACTCA CCAGAGCTCT 
TTAAGCTCCC ACAATGGTGC TAATCATATG GAACCAAACA CTGAGGATCA CAGAGATCCC 
ACTGACCTGC AGTCTAAGTC TCAAAGGACT GTGAACATGC CCACCAAAAG CCAGACACCT 
GAGGCAATTC CTGCACAGAC AGTTCCTCTG AAGAAGATCA AGCTAGAAGA GCCTAGCACA 
GTGTCAGATC AACACAGCAC ATCCTACCTA AGCAGCAGCG ACTTCTACCA GGATGCTTTG 
TCTACTTTAG CAAATGTTGT ATGTTCAACC ATCTCTGATC TGAAGGGTTT GGAGGAGAAG 
ACAGTTGGTC CTCAGACCTC AAATGCCTGT AATATTAAAA CTGAAAGAGA AGAAGAACCA 
TATCAGAGTC ATTCACATCA GGAAAGGACT TCTAACAGTC CAGCAGGTGC TGATAACTCC 
CATCCGAAAG ACCCAGTTCC GCTGACTAAT AATAGTGTCC AGTCTTTAGT TGAGCATAGG 
AACATAAGCA TTGATCAGGC AATAGCTATT GAGGCCTTGA CCCAGCTGGC AGCTACCCCA 
AAATCAGCTC CTGTCAAGGC AGATTCTAAA GTTCAGGACC CTCCACAAGA ATCTTGTAAC 
AAATTAGCAA ATGTTTCATC AAGCAGGCAA AGTCCGGTCC AAGAGGCTAA ACCCATATCT 
GAGGTCATCT CCAATAAAGT CTCTGTAATC AATTCACATC AGACTTCTGT AATTTGCAGC 
CCTCTGAACA GACAGGAAAA CCCCACCCAC CCCAAACTCT CCTTACAGGA TCTTTTAATA 
GCTAGCTCAT ATGATAAGCT CTTGCATAGT CCAGAGAATG GACGAGTAAG TCAAGCACTT 
TGTAAAACAG AAAAGTTAGA TGGCACTTTT AAGACTTTTA AGCATGTTGA AAGGACGCAG 
ACCTCAAGGA GGAGAGATGA GGAGGAAGTT GCAGCTCAGT TGGTGCAACT TGCATTCATA 
ATTCAATCAA GAACCAATCC CATGTCTTCT GAGAACAGTC CCCCCAAAGG GATGCCAGTA 
CAGACCATAA AATACAACCA TCATGTCGTT GGACAAAATG TAAAAAAGCA ACGGAAACCA 
AGAACAACTC CCTCAAGTCC CAGGATATCA AAGAAAAAAG CCTCTGAGGT TGAGGGAGGC 
AACCATAGGA TACCACTAGC CAAAAGGACA CCCAATAGCA AACCCCCTTT CAAGACAAAA 
GCCCAGCATG TGGCCTTGCA GGAAAAGCCC AAACCACAAC CCAAGTGGAG CCCGTTTCTC 
CCACAGACTC AGATAGACCT GAAAAAATAC CTAGCACAGG CTCATCATGA GAAACATCAG 
CTGTTCCATT TTAGCAGCAA GAGACAAGAC TTGGACATGC AGACTCTCAA GAGTTCCGGA 
AATCAGAAAG CTTTTACTTA CAAAACAGAC CACGCAGCTC TGAGTCATTC CCATGTTAAC 
CAATTCAGAT CCAACGGACA TTGTCACAGT CTCACAAATG GTCATCTAGG TGCTACACAT 
GGCCAGAAGT ATGAATGTGA AGAACATTTG ATTTCTCAGG TATCGAAAAC ATACAGTGTG 
CTAAATCATG GTGCTAATCC ACAAGCCCAG TATGCCATGC CTAGTGTCCC TGGTGCAGAT 
CCATTGCCAG GGAGTCCAGG GAGGTGCCAT AAAGTAAACA GCCTAAGCTG TGAGCCGCGG 
CAGACTTCCT TTGGTCAGAA TGGACGTTAC AAAGTGGACA CGGCTGGCGC TGTCACCGTT 
TTGTCTGTGT CAGCTGGAAA CATGGAAAAT GCTAACACTG AATCCGTTGA AGAAAACACT 
CCCACCAAGC ATATGCTTAA CAGCTTTTTG GAATCTCCGT TGAAGTACTT GGACACTCCA 
ACCAAGAATC TAATCAACAC ACCCTCCAAA AAAACTCCGG AGCTCCCCTC CTGTGACTGT 
ATGGAGCAAA TCATTGAAAA AGAGGAGGGT CCATTCTACA CACACCTTGG CTCTGGGCCA 
ACTGTAGCAG CAGTCCGAGA GATGATGGAG GACAGGTATG GGGAGAAGGG CAAAGCAGTG 
CGTGTGGAAG TTGTAGTTTA TACTGGGAGA GAAGGAAGGA GCTCCCAGGG CTGTCCAATA 
GCCAAATGGG TGATCCGTCG GGCCAGTGAG GAAGAGAAGC TGCTGTGCCT AGTGCGCCAG 
CGTGCAGGTC ACCGCTGTCA GAGCGCAGTA GTGGTAATCC TCATCCTGGC CTGGGAGGGG 
ATTCCACGCA ACATGGCCGA CAGACTGTAC CAGGAGCTTA CCCAGACCCT CTATAAATAT 
GGCTCGCCCA CCAGCCGCCG CTGCGCCCTT AATGAAGATC GTACCTGTGC CTGTCAGGGG 
CTGGACCCAG ATACCTGTGG AGCGTCCTTC TCTTTCGGCT GCTCGTGGAG TATGTATTTT 
AACGGCTGCA AGTTTGCCCG GAGCAAAGTG CCTAGAAAGT TCCGGCTGCA AGGAGACTAC 
CCAGAGGAGG AAGAAAAGAT CGAAAGCAAC CTCCTAAATC TGGCAACTGA TGTTGCACCT 
TTGTATAAGA AACTTGCTCC TGAAGCCTAT GAAAACCAGG TGGAGCAGGA GCAGTCAGGG 
CAAAACTGTC GGCTGGGCAG GAGAGAGGGT CGGCCATTTT CTGGTGTGAC AGCATGTGTG 
GACTTTTGTG CTCATGCTCA CAGGGACACA CACAACATGC ACAACGGGAG TACTGTGGTA 
TGCACTTTAA CTAAGGAGGA TAACCGTGCT GTAAGAAACA TTCCAGAGGA TGAGCAGCTG 
CATGTCCTGC CTCTTTACAA GATCTCGGAG ACTGATGAGT TCGGTCGTGT GGAGGGTCAG 
TGGGCTAAGA TACAGACTGG AGCTCTGCAG GTCCTCTCTT CCTTCCCAAG AGAGGTGCGT 
CTGCAGGCAG AGCCCGTCAA GTCTGCCCGT AAAAGGAGAC AAGAGGCCAA GAGAGAGCGA 
GAGAAGCAGA GAAACCAGGA GAAGAAGCAG GTCACACCAG TGAAAGTGAA GAACGAGCCC 
CTCAAAGGTT TTAAAAACTC TTCCTCTGAG CTTTCACCGT GTTTCAAAAC AGAAGCATGT 
TCTCCAACTC CTTATTGTTC CCCAATGCGA CTACCTAGGA CTCCAGACAA GGGGAGCCAC 
TTACTGGACT CGGCCAGTCC TTACAGGAGC ACATCATGTA CTCCTCCATT AAGCTCTACC 
AGAGACACAG CAGCACTTTC GTCCTCCCCT CACACCGTCC TCCCTGTGCC ACAATATCAG 
AATGTTGGAG AACTGGAGCA GAGCAATGAA TCTTCCCTCC TCAGACAGAG ACCAGGGTTA 
GAAGCAGCAA GACACAGCTC CCCTGAGCTC TCAGAACAGA AACCATTCAG AAACCAGCAG 
CAGATGGGCG GATCTTTGGG TTTCCGTGAC AAATGTTTTG CAGTAAAACC GGAGCCTGAG 
GAGGTCCGTT GTTTTCAAGG AGGAGCTGCT CCCCGACTGC TCTCCCCTTC CCGGGCTGAG 
GGTCTCCACA GCCGCCTGAA CTTTCGCCAA GCACAGCCGA GCCTGGATGA CCACCACGGG 
CCGCCGGTCA CCCCAAGGCC CCTGACCCCT GAGGAGGTCA AGGCAGAAGA GGTGTGGTCA 
GACAGCGAGC ACAACTTCCT AGATGGAGAC ATAGGCGGGG TCGCGGTGGC CCCCTCTCAC 
GGCTCCATCC TTATCGAGTG TGCCCGGCGG GAGCTGCACG CTACCACTCC CATTCTGCGG 
CCCAACCGCA GCCATCCCAC CCGAATCTCT CTGGTCTTCT ACCAGCACAA GAGCCTTAAC 
GTGCCTGGTC ACGGGCTGCT CCGGTGGGAA GCCAAGATGG CGGAGAAAGC CAGGGAGAAA 
GAGGAGGAGG CTATGCGGCT GGGACTAGAG ACGGAAGGTC TTGGGTCAGC TAAGACCAGA 
AAATCACAGC CGGCAGCGGG GAGCGACACG GAAAGTGGTG AGGAGGATGT GTGGCAGGAC 
GAGAGGGAGA AGCTACAGGT CCCTACCCGT CAGTCACTAA CAGCTACTCG GGACGGCCTC 
GTCACATCAG CCCCCTACTC CCTTACCCGG GTCACCGGGC CTTACAACCG CTGGATGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC103033153 ( XM_007240272.2 ) cDNA ORF clone, Astyanax mexicanus(Mexican tetra) -> XP_007240334.1 Astyanax mexicanus methylcytosine dioxygenase TET3-like (LOC103033153), transcript variant X1, mRNA.

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CloneID	OAs24334
Clone ID Related Accession (Same CDS sequence)	XM_007240272.2
Accession Version	XM_007240272.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5811bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1455033600000
Organism	Astyanax mexicanus(Mexican tetra)
Product	methylcytosine dioxygenase TET3-like isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_006739483.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Feb 11, 2016 this sequence version replaced XM_007240272.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Astyanax mexicanus Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCTCGTC CTCCCAAACC ACAAAGGAAG GTGCATCCCC CAGCTAAGCG AAATGTGAGG 
AAGTTGAGGG CGTCCACTGA TAAAAAGCGA GTAGTAAATT CAAACAAGCC CAAGGGTCCA 
GCAACACGTA CAAGACAGGC GAAGAGCAGG AAATCCACAG CCAAGAAACA GGCAACCAAG 
AAGCTCCAGG CAGGTGCTGC TGATAGCAAG TCTGCCGTAA AGGCATCCCA GGAAAGAGCA 
AGCAGAAGCA AGAACACATC TAACTCAACA GAATCTGTGA GAATCTCCAG ACACACGTGT 
AACTCTCTGT CACAGCATGA CCCGGCTGCA GACCTCAGGA GCAGAACGAG GTCTTTTCGA 
GGCAGCCTGT TGTTCCAGAC TTTCCCACAG GAAGTAAAGC CAACAAGGAG GGCGCCCTCA 
AGCAGAGGGA GAGGTCAGCA GGCAAGGGAA CAGGATAATT TCAAAATCAC AGAATGTAAT 
GACCCTAATA GTGATGATCA ACAAAAGAAA GCACTTGCTA CCAATGGAGA GGCTCAACTT 
GTTAACAGAA ACTCTGATGA AACAGCAGAG GAGGAGCACA GAGAACCTCT TGTTCACGAG 
ACTCCTGCGG TGGTGGATGA GGAGGAGGTG GCTGTGGCTG CGGTCACTGC AGAAACAGTG 
CAAAGTGAAA ACAGCCTAGC CTTCATTGAT AACACTGAAC AACCAAGCAC AACTAATGAG 
TCAGATGACG CATTTGTGAA AGAGCATGAC CCAGCTGTTA AAGTGGACTA CGTTTGTGAA 
ACTGATACCT CAGGCCCAAC AGATCCTCCT CCTTCAGACC TTCAGTCTTG TGACGTGGCT 
ATTCGAAGCA CTGTAGACGA TTCTTCTCAA CCAGATGTGT CTGTGGCATC TCCCGTATGT 
GAAATTCCCA AAGAATCACC AGCACTTCTG GAAGCTACAG ATGTGAAGTA TGCTGAGAAC 
GGAGAGCTGG ACAGCACTGA CGACTTAAAG ACCAAAGATG AGAATTATGA CAATGACAAT 
GATGAGTTTG CAAGCAAGAA CGAAGGAGCA CTTTCACTCC TCTCACTGAG CACTGGTGCT 
TATAGTGGCT TTCTGTCCAC TCTCGACTGT GGGCCTTCAA CAACAGCATC ACTTGACAGC 
AGCAAAGTGT CCAGCAGCAC AGATAGCACC CGATTTTCAT TTGATACCGA GTCTGAAGCA 
GGGAGTTTAG AGCTAGCTCT AGAGCACAGA GCTGCTGGTG CTTCTCCTCT GCTGGATGTG 
GATATAAGGC AGCGCTTACT GCAAGCTCAG GTGAGAAAAG AGAGGAAGAA GAGGAGTAGA 
TGTGGGGCCT GCGGACCCTG CCTCCTCAAG TACAGCTGTG GGCAGTGCAG CTGCTGCCTA 
AATCGAAAGA CTGGCCATCA GATCTGTAAG CTCAGAAAAT GTGTGATGCT GAAGAAAAGA 
CCCTGGCAAG TTACAGCTGG AGAGGTGGTG TGCAAGAATA TTCCCAAATC ACGCAAAAGG 
AACAGAGTGT CCAAGGTTGA CTCAGAGGGC ATCTTAGTAA ATGGCACTAA TTCAGAGCAG 
ATGGAAGAGA CCTGTGTGGT GCCAGAGGAA GAGGCGCGTG ACGTATCTCA AGCACACTCC 
GCTCCACCTG ACTCTCCCAC GCCTCTCGGG CACGACACTG AACCAGCACC AGCCGAAAGA 
GCCCTTCAGC CTGCTGTCAG CCACCATGCA CTTCTTACTC ACCAGAGCTC TTTAAGCTCC 
CACAATGGTG CTAATCATAT GGAACCAAAC ACTGAGGATC ACAGAGATCC CACTGACCTG 
CAGTCTAAGT CTCAAAGGAC TGTGAACATG CCCACCAAAA GCCAGACGCC TGAGGCAATT 
CCTGCACAGA CAGTTCCTCT GAAGAAGATC AAGCTAGAAG AGCCTAGCAC AGTGTCAGAT 
CAACACAGCA CATCCTACCT AAGCAGCAGC GACTTCTACC AGGATGCTTT GTCTACTTTA 
GCAAATGTTG TATGTTCAAC CATCTCTGAT CTGAAGGGTT TGGAGGAGAA GACAGTTGGT 
CCTCAGACCT CAAATGCCTG TAATATTAAA ACTGAAAGAG AAGAAGAACC ATATCAGAGT 
CATTCACATC AGGAAAGGAC TTCTAACAGT CCAGCAGGTG CTGATAACTC CCATCCGAAA 
GACCCAGTTC CGCTGACTAA TAATAGTGTC CAGTCTTTAG TTGAGCATAG GAACATAAGC 
ATTGATCAGG CAATAGCTAT TGAGGCCTTG ACCCAGCTGG CAGCTACCCC AAAATCAGCT 
CCTGTCAAGG CAGATTCTAA AGTTCAGGAC CCTCCACAAG AATCTTGTAA CAAATTAGCA 
AATGTTTCAT CAAGCAGGCA AAGCCCGGTC CAAGAGGCTA AACCCATATC TGAGGTCATC 
TCCAATAAAG TCTCTGTAAT CAATTCACAT CAGACTTCTG TAATTTGCAG CCCTCTGAAC 
AGACAGGAAA ACCCCACCCA CCCCAAACTC TCCTTACAGG ATCTTTTAAT AGCTAGCTCA 
TATGATAAGC TCTTGCATAG TCCAGAGAAT GGACGAGTAA GTCAAGCACT TTGTAAAACA 
GAAACGTTAG ATGGCACTTT TAAGACTTTT AAGCATGTTG AAAGGACGCA GACCTCAAGG 
AGGAGAGATG AGGAGGAAGT TGCAGCTCAG TTGGTGCAAC TTGCATTCAT AATTCAATCA 
AGAACCAATC CCATGTCTTC TGAGAACAGT CCCCCCAAAG GGATGCCAGT ACAGACCATA 
AAATACAACC ATCATGTCGT TGGACAAAAT GTAAAAAAGC AACGGAAACC AAGAACAACT 
CCCTCAAGTC CCAGGATATC AAAGAAAAAA GCCTCTGAGG TTGAGGGAGG CAACCATAGG 
ATACCACTAG CCAAAAGGAC ACCCAATAGC AAACCCCCTT TCAAGACAAA AGCCCAGCAT 
GTGGCCTTGC AGGAAAAGCC CAAACCACAA CCCAAGTGGA GCCCGTTTCT CCCACAGACT 
CAGATAGACC TGAAAAAATA CCTAGCACAG GCTCATCATG AGAAACATCA GCTGTTCCAT 
TTTAGCAGCA AGAGACAAGA GTTGGACATG CAGACTCTCA AGAGTTCCGG AAATCAGAAA 
GCTTTTACTT ACAAAACAGA CCACGCAGCT CTGAGTCATT CCCATGTTAA CCAATTCAGA 
TCCAACGGAC ATTGTCACAG TCTCACAAAT GGTCATCTAG GTGCTACACA TGGCCAGAAG 
TATGAATGTG AAGAACATTT GATTTCTCAG GTATCGAAAA CATACAGTGT GCTAAATCAT 
GGTGCTAATC CACAAGCCCA GTATGCCATG CCTAGTGTCC CTGGTGCAGA TCCATTGCCA 
GGGAGTCCAG GGAGGTGCCA TAAAGTAAAC AGCCTAAGCT GTGAGCCGCG GCAGACTTCC 
TTTGGTCAGA ATGGACGTTA CAAAGTGGAC ACGGCTGGCG CTGTCACCGT TTTGTCTGTG 
TCAGCTGGAA ACATGGAAAA TGCTAACACT GAATCCGTTG AAGAAAACAC TCCCACCAAG 
CATATGCTTA ACAGCTTTTT GGAATCTCCG TTGAAGTACT TGGACACTCC AACCAAGAAT 
CTAATCAACA CACCCTCCAA AAAAACTCCG GAGCTCCCCT CCTGTGACTG TATGGAGCAA 
ATCATTGAAA AAGAGGAGGG TCCATTCTAC ACACACCTTG GCTCTGGGCC AACTGTAGCA 
GCAGTCCGAG AGATGATGGA GGACAGGTAT GGGGAGAAGG GCAAAGCAGT GCGTGTGGAA 
GTTGTAGTTT ATACTGGGAG AGAAGGAAGG AGCTCCCAGG GCTGTCCAAT AGCCAAATGG 
GTGATCCGTC GGGCCAGTGA GGAAGAGAAG CTGCTGTGCC TAGTGCGCCA GCGTGCAGGT 
CACCGCTGTC AGAGCGCAGT AGTGGTAATC CTCATCCTGG CCTGGGAGGG GATTCCACGC 
AACATGGCCG ACAGACTGTA CCAGGAGCTT ACCCAGACCC TCTATAAATA TGGCTCGCCC 
ACCAGCCGCC GCTGCGCCCT TAATGAAGAT CGTACCTGTG CCTGTCAGGG GCTGGACCCA 
GATACCTGTG GAGCGTCCTT CTCTTTCGGC TGCTCGTGGA GTATGTATTT TAACGGCTGC 
AAGTTTGCCC GGAGCAAAGT GCCTAGAAAG TTCCGGCTGC AAGGAGACTA CCCAGAGGAG 
GAAGAAAAGA TCGAAAGCAA CCTCCTAAAT CTGGCAACTG ATGTTGCACC TTTGTATAAG 
AAACTTGCTC CTGAAGCCTA TGAAAACCAG GTGGAGCAGG AGCAGTCAGG GCAAAACTGT 
CGGCTGGGCA GGAGAGAGGG TCGGCCATTT TCTGGTGTGA CAGCATGTGT GGACTTTTGT 
GCTCATGCTC ACAGGGACAC ACACAACATG CACAACGGGA GTACTGTGGT ATGCACTTTA 
ACTAAGGAGG ATAACCGTGC TGTAAGAAAC ATTCCAGAGG ATGAGCAGCT GCATGTCCTG 
CCTCTTTACA AGATCTCGGA GACTGATGAG TTCGGTCGTG TGGAGGGTCA GTGGGCTAAG 
ATACAGACTG GAGCTCTGCA GGTCCTCTCT TCCTTCCCAA GAGAGGTGCG TCTGCAGGCA 
GAGCCCGTCA AGTCTGCCCG TAAAAGGAGA CAAGAGGCCA AGAGAGAGCG AGAGAAGCAG 
AGAAACCAGG AGAAGAAGCA GGTCACACCA GTGAAAGTGA AGAACGAGCC CCTCAAAGGT 
TTTAAAAACT CTTCCTCTGA GCTTTCACCG TGTTTCAAAA CAGAAGCATG TTCTCCAACT 
CCTTATTGTT CCCCAATGCG ACTACCTAGG ACTCCAGACA AGGGGAGCCA CTTACTGGAC 
TCGGCCAGTC CTTACAGGAG CACATCATGT ACTCCTCCAT TAAGCTCTAC CAGAGACACA 
GCAGCACTTT CGTCCTCCCC TCACACCGTC CTCCCTGTGC CACAATATCA GAATGTTGGA 
GAACTGGAGC AGAGCAATGA ATCTTCCCTC CTCAGACAGA GACCAGGGTT AGAAGCAGCA 
AGACACAGCT CCCCTGAGCT CTCAGAACAG AAACCATTCA GAAACCAGCA GCAGATGGGC 
GGATCTTTGG GTTTCCGTGA CAAATGTTTT GCAGTAAAAC CGGAGCCTGA GGAGGTCCGT 
TGTTTTCAAG GAGGAGCTGC TCCCCGACTG CTCTCCCCTT CCCGGGCTGA GGGTCTCCAC 
AGCCGCCTGA ACTTTCGCCA AGCACAGCCG AGCCTGGATG ACCACCACGG GCCGCCGGTC 
ACCCCAAGGC CCCTGACCCC TGAGGAGGTC AAGGCAGAAG AGGTGTGGTC AGACAGCGAG 
CACAACTTCC TAGATGGAGA CATAGGCGGG GTCGCGGTGG CCCCCTCTCA CGGCTCCATC 
CTTATCGAGT GTGCCCGGCG GGAGCTGCAC GCTACCACTC CCATTCTGCG GCCCAACCGC 
AGCCATCCCA CCCGAATCTC TCTGGTCTTC TACCAGCACA AGAGCCTTAA CGTGCCTGGT 
CACGGGCTGC TCCGGTGGGA AGCCAAGATG GCGGAGAAAG CCAGGGAGAA AGAGGAGGAG 
GCTATGCGGC TGGGACTAGA GACGGAAGGT CTTGGGTCAG CTAAGACCAG AAAATCACAG 
CCGGCAGCGG GGAGCGACAC GGAAAGCGGT GAGGAGGATG TGTGGCAGGA CGAGAGGGAG 
AAGCTACAGG TCCCTACCCG TCAGTCACTA ACAGCTACTC GGGACGGCCT CGTCACATCA 
GCCCCCTACT CCCTTACCCG GGTCACCGGG CCTTACAACC GCTGGATGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_007240334.1
CDS	457..6267
Translation	MPRPPKPQRKVHPPAKRNVRKLRASTDKKRVVNSNKPKGPATRTRQAKSRKSTAKKQATKKLQAGAADSKSAVKASQERASRSKNTSNSTESVRISRHTCNSLSQHDPAADLRSRTRSFRGSLLFQTFPQEVKPTRRAPSSRGRGQQAREQDNFKITECNDPNSDDQQKKALATNGEAQLVNRNSDETAEEEHREPLVHETPAVVDEEEVAVAAVTAETVQSENSLAFIDNTEQPSTTNESDDAFVKEHDPAVKVDYVCETDTSGPTDPPPSDLQSCDVAIRSTVDDSSQPDVSVASPVCEIPKESPALLEATDVKYAENGELDSTDDLKTKDENYDNDNDEFASKNEGALSLLSLSTGAYSGFLSTLDCGPSTTASLDSSKVSSSTDSTRFSFDTESEAGSLELALEHRAAGASPLLDVDIRQRLLQAQVRKERKKRSRCGACGPCLLKYSCGQCSCCLNRKTGHQICKLRKCVMLKKRPWQVTAGEVVCKNIPKSRKRNRVSKVDSEGILVNGTNSEQMEETCVVPEEEARDVSQAHSAPPDSPTPLGHDTEPAPAERALQPAVSHHALLTHQSSLSSHNGANHMEPNTEDHRDPTDLQSKSQRTVNMPTKSQTPEAIPAQTVPLKKIKLEEPSTVSDQHSTSYLSSSDFYQDALSTLANVVCSTISDLKGLEEKTVGPQTSNACNIKTEREEEPYQSHSHQERTSNSPAGADNSHPKDPVPLTNNSVQSLVEHRNISIDQAIAIEALTQLAATPKSAPVKADSKVQDPPQESCNKLANVSSSRQSPVQEAKPISEVISNKVSVINSHQTSVICSPLNRQENPTHPKLSLQDLLIASSYDKLLHSPENGRVSQALCKTETLDGTFKTFKHVERTQTSRRRDEEEVAAQLVQLAFIIQSRTNPMSSENSPPKGMPVQTIKYNHHVVGQNVKKQRKPRTTPSSPRISKKKASEVEGGNHRIPLAKRTPNSKPPFKTKAQHVALQEKPKPQPKWSPFLPQTQIDLKKYLAQAHHEKHQLFHFSSKRQELDMQTLKSSGNQKAFTYKTDHAALSHSHVNQFRSNGHCHSLTNGHLGATHGQKYECEEHLISQVSKTYSVLNHGANPQAQYAMPSVPGADPLPGSPGRCHKVNSLSCEPRQTSFGQNGRYKVDTAGAVTVLSVSAGNMENANTESVEENTPTKHMLNSFLESPLKYLDTPTKNLINTPSKKTPELPSCDCMEQIIEKEEGPFYTHLGSGPTVAAVREMMEDRYGEKGKAVRVEVVVYTGREGRSSQGCPIAKWVIRRASEEEKLLCLVRQRAGHRCQSAVVVILILAWEGIPRNMADRLYQELTQTLYKYGSPTSRRCALNEDRTCACQGLDPDTCGASFSFGCSWSMYFNGCKFARSKVPRKFRLQGDYPEEEEKIESNLLNLATDVAPLYKKLAPEAYENQVEQEQSGQNCRLGRREGRPFSGVTACVDFCAHAHRDTHNMHNGSTVVCTLTKEDNRAVRNIPEDEQLHVLPLYKISETDEFGRVEGQWAKIQTGALQVLSSFPREVRLQAEPVKSARKRRQEAKREREKQRNQEKKQVTPVKVKNEPLKGFKNSSSELSPCFKTEACSPTPYCSPMRLPRTPDKGSHLLDSASPYRSTSCTPPLSSTRDTAALSSSPHTVLPVPQYQNVGELEQSNESSLLRQRPGLEAARHSSPELSEQKPFRNQQQMGGSLGFRDKCFAVKPEPEEVRCFQGGAAPRLLSPSRAEGLHSRLNFRQAQPSLDDHHGPPVTPRPLTPEEVKAEEVWSDSEHNFLDGDIGGVAVAPSHGSILIECARRELHATTPILRPNRSHPTRISLVFYQHKSLNVPGHGLLRWEAKMAEKAREKEEEAMRLGLETEGLGSAKTRKSQPAAGSDTESGEEDVWQDEREKLQVPTRQSLTATRDGLVTSAPYSLTRVTGPYNRWM

Target ORF information:

RefSeq Version	XM_007240272.2
Organism	Astyanax mexicanus(Mexican tetra)
Definition	Astyanax mexicanus methylcytosine dioxygenase TET3-like (LOC103033153), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_007240272.2

ORF Insert Sequence:

ATGCCTCGTC CTCCCAAACC ACAAAGGAAG GTGCATCCCC CAGCTAAGCG AAATGTGAGG 
AAGTTGAGGG CGTCCACTGA TAAAAAGCGA GTAGTAAATT CAAACAAGCC CAAGGGTCCA 
GCAACACGTA CAAGACAGGC GAAGAGCAGG AAATCCACAG CCAAGAAACA GGCAACCAAG 
AAGCTCCAGG CAGGTGCTGC TGATAGCAAG TCTGCCGTAA AGGCATCCCA GGAAAGAGCA 
AGCAGAAGCA AGAACACATC TAACTCAACA GAATCTGTGA GAATCTCCAG ACACACGTGT 
AACTCTCTGT CACAGCATGA CCCGGCTGCA GACCTCAGGA GCAGAACGAG GTCTTTTCGA 
GGCAGCCTGT TGTTCCAGAC TTTCCCACAG GAAGTAAAGC CAACAAGGAG GGCGCCCTCA 
AGCAGAGGGA GAGGTCAGCA GGCAAGGGAA CAGGATAATT TCAAAATCAC AGAATGTAAT 
GACCCTAATA GTGATGATCA ACAAAAGAAA GCACTTGCTA CCAATGGAGA GGCTCAACTT 
GTTAACAGAA ACTCTGATGA AACAGCAGAG GAGGAGCACA GAGAACCTCT TGTTCACGAG 
ACTCCTGCGG TGGTGGATGA GGAGGAGGTG GCTGTGGCTG CGGTCACTGC AGAAACAGTG 
CAAAGTGAAA ACAGCCTAGC CTTCATTGAT AACACTGAAC AACCAAGCAC AACTAATGAG 
TCAGATGACG CATTTGTGAA AGAGCATGAC CCAGCTGTTA AAGTGGACTA CGTTTGTGAA 
ACTGATACCT CAGGCCCAAC AGATCCTCCT CCTTCAGACC TTCAGTCTTG TGACGTGGCT 
ATTCGAAGCA CTGTAGACGA TTCTTCTCAA CCAGATGTGT CTGTGGCATC TCCCGTATGT 
GAAATTCCCA AAGAATCACC AGCACTTCTG GAAGCTACAG ATGTGAAGTA TGCTGAGAAC 
GGAGAGCTGG ACAGCACTGA CGACTTAAAG ACCAAAGATG AGAATTATGA CAATGACAAT 
GATGAGTTTG CAAGCAAGAA CGAAGGAGCA CTTTCACTCC TCTCACTGAG CACTGGTGCT 
TATAGTGGCT TTCTGTCCAC TCTCGACTGT GGGCCTTCAA CAACAGCATC ACTTGACAGC 
AGCAAAGTGT CCAGCAGCAC AGATAGCACC CGATTTTCAT TTGATACCGA GTCTGAAGCA 
GGGAGTTTAG AGCTAGCTCT AGAGCACAGA GCTGCTGGTG CTTCTCCTCT GCTGGATGTG 
GATATAAGGC AGCGCTTACT GCAAGCTCAG GTGAGAAAAG AGAGGAAGAA GAGGAGTAGA 
TGTGGGGCCT GCGGACCCTG CCTCCTCAAG TACAGCTGTG GGCAGTGCAG CTGCTGCCTA 
AATCGAAAGA CTGGCCATCA GATCTGTAAG CTCAGAAAAT GTGTGATGCT GAAGAAAAGA 
CCCTGGCAAG TTACAGCTGG AGAGGTGGTG TGCAAGAATA TTCCCAAATC ACGCAAAAGG 
AACAGAGTGT CCAAGGTTGA CTCAGAGGGC ATCTTAGTAA ATGGCACTAA TTCAGAGCAG 
ATGGAAGAGA CCTGTGTGGT GCCAGAGGAA GAGGCGCGTG ACGTATCTCA AGCACACTCC 
GCTCCACCTG ACTCTCCCAC GCCTCTCGGG CACGACACTG AACCAGCACC AGCCGAAAGA 
GCCCTTCAGC CTGCTGTCAG CCACCATGCA CTTCTTACTC ACCAGAGCTC TTTAAGCTCC 
CACAATGGTG CTAATCATAT GGAACCAAAC ACTGAGGATC ACAGAGATCC CACTGACCTG 
CAGTCTAAGT CTCAAAGGAC TGTGAACATG CCCACCAAAA GCCAGACGCC TGAGGCAATT 
CCTGCACAGA CAGTTCCTCT GAAGAAGATC AAGCTAGAAG AGCCTAGCAC AGTGTCAGAT 
CAACACAGCA CATCCTACCT AAGCAGCAGC GACTTCTACC AGGATGCTTT GTCTACTTTA 
GCAAATGTTG TATGTTCAAC CATCTCTGAT CTGAAGGGTT TGGAGGAGAA GACAGTTGGT 
CCTCAGACCT CAAATGCCTG TAATATTAAA ACTGAAAGAG AAGAAGAACC ATATCAGAGT 
CATTCACATC AGGAAAGGAC TTCTAACAGT CCAGCAGGTG CTGATAACTC CCATCCGAAA 
GACCCAGTTC CGCTGACTAA TAATAGTGTC CAGTCTTTAG TTGAGCATAG GAACATAAGC 
ATTGATCAGG CAATAGCTAT TGAGGCCTTG ACCCAGCTGG CAGCTACCCC AAAATCAGCT 
CCTGTCAAGG CAGATTCTAA AGTTCAGGAC CCTCCACAAG AATCTTGTAA CAAATTAGCA 
AATGTTTCAT CAAGCAGGCA AAGCCCGGTC CAAGAGGCTA AACCCATATC TGAGGTCATC 
TCCAATAAAG TCTCTGTAAT CAATTCACAT CAGACTTCTG TAATTTGCAG CCCTCTGAAC 
AGACAGGAAA ACCCCACCCA CCCCAAACTC TCCTTACAGG ATCTTTTAAT AGCTAGCTCA 
TATGATAAGC TCTTGCATAG TCCAGAGAAT GGACGAGTAA GTCAAGCACT TTGTAAAACA 
GAAACGTTAG ATGGCACTTT TAAGACTTTT AAGCATGTTG AAAGGACGCA GACCTCAAGG 
AGGAGAGATG AGGAGGAAGT TGCAGCTCAG TTGGTGCAAC TTGCATTCAT AATTCAATCA 
AGAACCAATC CCATGTCTTC TGAGAACAGT CCCCCCAAAG GGATGCCAGT ACAGACCATA 
AAATACAACC ATCATGTCGT TGGACAAAAT GTAAAAAAGC AACGGAAACC AAGAACAACT 
CCCTCAAGTC CCAGGATATC AAAGAAAAAA GCCTCTGAGG TTGAGGGAGG CAACCATAGG 
ATACCACTAG CCAAAAGGAC ACCCAATAGC AAACCCCCTT TCAAGACAAA AGCCCAGCAT 
GTGGCCTTGC AGGAAAAGCC CAAACCACAA CCCAAGTGGA GCCCGTTTCT CCCACAGACT 
CAGATAGACC TGAAAAAATA CCTAGCACAG GCTCATCATG AGAAACATCA GCTGTTCCAT 
TTTAGCAGCA AGAGACAAGA GTTGGACATG CAGACTCTCA AGAGTTCCGG AAATCAGAAA 
GCTTTTACTT ACAAAACAGA CCACGCAGCT CTGAGTCATT CCCATGTTAA CCAATTCAGA 
TCCAACGGAC ATTGTCACAG TCTCACAAAT GGTCATCTAG GTGCTACACA TGGCCAGAAG 
TATGAATGTG AAGAACATTT GATTTCTCAG GTATCGAAAA CATACAGTGT GCTAAATCAT 
GGTGCTAATC CACAAGCCCA GTATGCCATG CCTAGTGTCC CTGGTGCAGA TCCATTGCCA 
GGGAGTCCAG GGAGGTGCCA TAAAGTAAAC AGCCTAAGCT GTGAGCCGCG GCAGACTTCC 
TTTGGTCAGA ATGGACGTTA CAAAGTGGAC ACGGCTGGCG CTGTCACCGT TTTGTCTGTG 
TCAGCTGGAA ACATGGAAAA TGCTAACACT GAATCCGTTG AAGAAAACAC TCCCACCAAG 
CATATGCTTA ACAGCTTTTT GGAATCTCCG TTGAAGTACT TGGACACTCC AACCAAGAAT 
CTAATCAACA CACCCTCCAA AAAAACTCCG GAGCTCCCCT CCTGTGACTG TATGGAGCAA 
ATCATTGAAA AAGAGGAGGG TCCATTCTAC ACACACCTTG GCTCTGGGCC AACTGTAGCA 
GCAGTCCGAG AGATGATGGA GGACAGGTAT GGGGAGAAGG GCAAAGCAGT GCGTGTGGAA 
GTTGTAGTTT ATACTGGGAG AGAAGGAAGG AGCTCCCAGG GCTGTCCAAT AGCCAAATGG 
GTGATCCGTC GGGCCAGTGA GGAAGAGAAG CTGCTGTGCC TAGTGCGCCA GCGTGCAGGT 
CACCGCTGTC AGAGCGCAGT AGTGGTAATC CTCATCCTGG CCTGGGAGGG GATTCCACGC 
AACATGGCCG ACAGACTGTA CCAGGAGCTT ACCCAGACCC TCTATAAATA TGGCTCGCCC 
ACCAGCCGCC GCTGCGCCCT TAATGAAGAT CGTACCTGTG CCTGTCAGGG GCTGGACCCA 
GATACCTGTG GAGCGTCCTT CTCTTTCGGC TGCTCGTGGA GTATGTATTT TAACGGCTGC 
AAGTTTGCCC GGAGCAAAGT GCCTAGAAAG TTCCGGCTGC AAGGAGACTA CCCAGAGGAG 
GAAGAAAAGA TCGAAAGCAA CCTCCTAAAT CTGGCAACTG ATGTTGCACC TTTGTATAAG 
AAACTTGCTC CTGAAGCCTA TGAAAACCAG GTGGAGCAGG AGCAGTCAGG GCAAAACTGT 
CGGCTGGGCA GGAGAGAGGG TCGGCCATTT TCTGGTGTGA CAGCATGTGT GGACTTTTGT 
GCTCATGCTC ACAGGGACAC ACACAACATG CACAACGGGA GTACTGTGGT ATGCACTTTA 
ACTAAGGAGG ATAACCGTGC TGTAAGAAAC ATTCCAGAGG ATGAGCAGCT GCATGTCCTG 
CCTCTTTACA AGATCTCGGA GACTGATGAG TTCGGTCGTG TGGAGGGTCA GTGGGCTAAG 
ATACAGACTG GAGCTCTGCA GGTCCTCTCT TCCTTCCCAA GAGAGGTGCG TCTGCAGGCA 
GAGCCCGTCA AGTCTGCCCG TAAAAGGAGA CAAGAGGCCA AGAGAGAGCG AGAGAAGCAG 
AGAAACCAGG AGAAGAAGCA GGTCACACCA GTGAAAGTGA AGAACGAGCC CCTCAAAGGT 
TTTAAAAACT CTTCCTCTGA GCTTTCACCG TGTTTCAAAA CAGAAGCATG TTCTCCAACT 
CCTTATTGTT CCCCAATGCG ACTACCTAGG ACTCCAGACA AGGGGAGCCA CTTACTGGAC 
TCGGCCAGTC CTTACAGGAG CACATCATGT ACTCCTCCAT TAAGCTCTAC CAGAGACACA 
GCAGCACTTT CGTCCTCCCC TCACACCGTC CTCCCTGTGC CACAATATCA GAATGTTGGA 
GAACTGGAGC AGAGCAATGA ATCTTCCCTC CTCAGACAGA GACCAGGGTT AGAAGCAGCA 
AGACACAGCT CCCCTGAGCT CTCAGAACAG AAACCATTCA GAAACCAGCA GCAGATGGGC 
GGATCTTTGG GTTTCCGTGA CAAATGTTTT GCAGTAAAAC CGGAGCCTGA GGAGGTCCGT 
TGTTTTCAAG GAGGAGCTGC TCCCCGACTG CTCTCCCCTT CCCGGGCTGA GGGTCTCCAC 
AGCCGCCTGA ACTTTCGCCA AGCACAGCCG AGCCTGGATG ACCACCACGG GCCGCCGGTC 
ACCCCAAGGC CCCTGACCCC TGAGGAGGTC AAGGCAGAAG AGGTGTGGTC AGACAGCGAG 
CACAACTTCC TAGATGGAGA CATAGGCGGG GTCGCGGTGG CCCCCTCTCA CGGCTCCATC 
CTTATCGAGT GTGCCCGGCG GGAGCTGCAC GCTACCACTC CCATTCTGCG GCCCAACCGC 
AGCCATCCCA CCCGAATCTC TCTGGTCTTC TACCAGCACA AGAGCCTTAA CGTGCCTGGT 
CACGGGCTGC TCCGGTGGGA AGCCAAGATG GCGGAGAAAG CCAGGGAGAA AGAGGAGGAG 
GCTATGCGGC TGGGACTAGA GACGGAAGGT CTTGGGTCAG CTAAGACCAG AAAATCACAG 
CCGGCAGCGG GGAGCGACAC GGAAAGCGGT GAGGAGGATG TGTGGCAGGA CGAGAGGGAG 
AAGCTACAGG TCCCTACCCG TCAGTCACTA ACAGCTACTC GGGACGGCCT CGTCACATCA 
GCCCCCTACT CCCTTACCCG GGTCACCGGG CCTTACAACC GCTGGATGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC103033153 ( XM_007240273.2 ) cDNA ORF clone, Astyanax mexicanus(Mexican tetra) -> XP_007240335.1 Astyanax mexicanus methylcytosine dioxygenase TET3-like (LOC103033153), transcript variant X2, mRNA.

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CloneID	OAs24335
Clone ID Related Accession (Same CDS sequence)	XM_007240273.2
Accession Version	XM_007240273.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5760bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1455033600000
Organism	Astyanax mexicanus(Mexican tetra)
Product	methylcytosine dioxygenase TET3-like isoform X2
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_006739483.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Feb 11, 2016 this sequence version replaced XM_007240273.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Astyanax mexicanus Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCTCGTC CTCCCAAACC ACAAAGGAAG GTGCATCCCC CAGCTAAGCG AAATGTGAGG 
AAGTTGAGGG CGTCCACTGA TAAAAAGCGA GTAGTAAATT CAAACAAGCC CAAGGGTCCA 
GCAACACGTA CAAGACAGGC GAAGAGCAGG AAATCCACAG CCAAGAAACA GGCAACCAAG 
AAGCTCCAGG CAGGTGCTGC TGATAGCAAG TCTGCCGTAA AGGCATCCCA GGAAAGAGCA 
AGCAGAAGCA AGAACACATC TAACTCAACA GAATCTGTGA GAATCTCCAG ACACACGTGT 
AACTCTCTGT CACAGCATGA CCCGGCTGCA GACCTCAGGA GCAGAACGAG GTCTTTTCGA 
GGCAGCCTGT TGTTCCAGAC TTTCCCACAG GAAGTAAAGC CAACAAGGAG GGCGCCCTCA 
AGCAGAGGGA GAGGTCAGCA GGCAAGGGAA CAGGATAATT TCAAAATCAC AGAATGTAAT 
GACCCTAATA GTGATGATCA ACAAAAGAAA GCACTTGCTA CCAATGGAGA GGCTCAACTT 
GTTAACAGAA ACTCTGATGA AACAGCAGAG GAGGAGCACA GAGAACCTCT TGTTCACGAG 
ACTCCTGCGG TGGTGGATGA GGAGGAGGTG GCTGTGGCTG CGGTCACTGC AGAAACAGTG 
CAAAGTGAAA ACAGCCTAGC CTTCATTGAT AACACTGAAC AACCAAGCAC AACTAATGAG 
TCAGATGACG CATTTGTGAA AGAGCATGAC CCAGCTGTTA AAGTGGACTA CGTTTGTGAA 
ACTGATACCT CAGGCCCAAC AGATCCTCCT CCTTCAGACC TTCAGTCTTG TGACGTGGCT 
ATTCGAAGCA CTGTAGACGA TTCTTCTCAA CCAGATGTGT CTGTGGCATC TCCCGTATGT 
GAAATTCCCA AAGAATCACC AGCACTTCTG GAAGCTACAG ATGTGAAGTA TGCTGAGAAC 
GGAGAGCTGG ACAGCACTGA CGACTTAAAG ACCAAAGATG AGAATTATGA CAATGACAAT 
GATGAGTTTG CAAGCAAGAA CGAAGGAGCA CTTTCACTCC TCTCACTGAG CACTGGTGCT 
TATAGTGGCT TTCTGTCCAC TCTCGACTGT GGGCCTTCAA CAACAGCATC ACTTGACAGC 
AGCAAAGTGT CCAGCAGCAC AGATAGCACC CGATTTTCAT TTGATACCGA GTCTGAAGCA 
GGGAGTTTAG AGCTAGCTCT AGAGCACAGA GCTGCTGGTG CTTCTCCTCT GCTGGATGTG 
GATATAAGGC AGCGCTTACT GCAAGCTCAG GTGAGAAAAG AGAGGAAGAA GAGGAGTAGA 
TGTGGGGCCT GCGGACCCTG CCTCCTCAAG TACAGCTGTG GGCAGTGCAG CTGCTGCCTA 
AATCGAAAGA CTGGCCATCA GATCTGTAAG CTCAGAAAAT GTGTGATGCT GAAGAAAAGA 
CCCTGGCAAG TTACAGCTGG AGAGGTTGAC TCAGAGGGCA TCTTAGTAAA TGGCACTAAT 
TCAGAGCAGA TGGAAGAGAC CTGTGTGGTG CCAGAGGAAG AGGCGCGTGA CGTATCTCAA 
GCACACTCCG CTCCACCTGA CTCTCCCACG CCTCTCGGGC ACGACACTGA ACCAGCACCA 
GCCGAAAGAG CCCTTCAGCC TGCTGTCAGC CACCATGCAC TTCTTACTCA CCAGAGCTCT 
TTAAGCTCCC ACAATGGTGC TAATCATATG GAACCAAACA CTGAGGATCA CAGAGATCCC 
ACTGACCTGC AGTCTAAGTC TCAAAGGACT GTGAACATGC CCACCAAAAG CCAGACGCCT 
GAGGCAATTC CTGCACAGAC AGTTCCTCTG AAGAAGATCA AGCTAGAAGA GCCTAGCACA 
GTGTCAGATC AACACAGCAC ATCCTACCTA AGCAGCAGCG ACTTCTACCA GGATGCTTTG 
TCTACTTTAG CAAATGTTGT ATGTTCAACC ATCTCTGATC TGAAGGGTTT GGAGGAGAAG 
ACAGTTGGTC CTCAGACCTC AAATGCCTGT AATATTAAAA CTGAAAGAGA AGAAGAACCA 
TATCAGAGTC ATTCACATCA GGAAAGGACT TCTAACAGTC CAGCAGGTGC TGATAACTCC 
CATCCGAAAG ACCCAGTTCC GCTGACTAAT AATAGTGTCC AGTCTTTAGT TGAGCATAGG 
AACATAAGCA TTGATCAGGC AATAGCTATT GAGGCCTTGA CCCAGCTGGC AGCTACCCCA 
AAATCAGCTC CTGTCAAGGC AGATTCTAAA GTTCAGGACC CTCCACAAGA ATCTTGTAAC 
AAATTAGCAA ATGTTTCATC AAGCAGGCAA AGCCCGGTCC AAGAGGCTAA ACCCATATCT 
GAGGTCATCT CCAATAAAGT CTCTGTAATC AATTCACATC AGACTTCTGT AATTTGCAGC 
CCTCTGAACA GACAGGAAAA CCCCACCCAC CCCAAACTCT CCTTACAGGA TCTTTTAATA 
GCTAGCTCAT ATGATAAGCT CTTGCATAGT CCAGAGAATG GACGAGTAAG TCAAGCACTT 
TGTAAAACAG AAACGTTAGA TGGCACTTTT AAGACTTTTA AGCATGTTGA AAGGACGCAG 
ACCTCAAGGA GGAGAGATGA GGAGGAAGTT GCAGCTCAGT TGGTGCAACT TGCATTCATA 
ATTCAATCAA GAACCAATCC CATGTCTTCT GAGAACAGTC CCCCCAAAGG GATGCCAGTA 
CAGACCATAA AATACAACCA TCATGTCGTT GGACAAAATG TAAAAAAGCA ACGGAAACCA 
AGAACAACTC CCTCAAGTCC CAGGATATCA AAGAAAAAAG CCTCTGAGGT TGAGGGAGGC 
AACCATAGGA TACCACTAGC CAAAAGGACA CCCAATAGCA AACCCCCTTT CAAGACAAAA 
GCCCAGCATG TGGCCTTGCA GGAAAAGCCC AAACCACAAC CCAAGTGGAG CCCGTTTCTC 
CCACAGACTC AGATAGACCT GAAAAAATAC CTAGCACAGG CTCATCATGA GAAACATCAG 
CTGTTCCATT TTAGCAGCAA GAGACAAGAG TTGGACATGC AGACTCTCAA GAGTTCCGGA 
AATCAGAAAG CTTTTACTTA CAAAACAGAC CACGCAGCTC TGAGTCATTC CCATGTTAAC 
CAATTCAGAT CCAACGGACA TTGTCACAGT CTCACAAATG GTCATCTAGG TGCTACACAT 
GGCCAGAAGT ATGAATGTGA AGAACATTTG ATTTCTCAGG TATCGAAAAC ATACAGTGTG 
CTAAATCATG GTGCTAATCC ACAAGCCCAG TATGCCATGC CTAGTGTCCC TGGTGCAGAT 
CCATTGCCAG GGAGTCCAGG GAGGTGCCAT AAAGTAAACA GCCTAAGCTG TGAGCCGCGG 
CAGACTTCCT TTGGTCAGAA TGGACGTTAC AAAGTGGACA CGGCTGGCGC TGTCACCGTT 
TTGTCTGTGT CAGCTGGAAA CATGGAAAAT GCTAACACTG AATCCGTTGA AGAAAACACT 
CCCACCAAGC ATATGCTTAA CAGCTTTTTG GAATCTCCGT TGAAGTACTT GGACACTCCA 
ACCAAGAATC TAATCAACAC ACCCTCCAAA AAAACTCCGG AGCTCCCCTC CTGTGACTGT 
ATGGAGCAAA TCATTGAAAA AGAGGAGGGT CCATTCTACA CACACCTTGG CTCTGGGCCA 
ACTGTAGCAG CAGTCCGAGA GATGATGGAG GACAGGTATG GGGAGAAGGG CAAAGCAGTG 
CGTGTGGAAG TTGTAGTTTA TACTGGGAGA GAAGGAAGGA GCTCCCAGGG CTGTCCAATA 
GCCAAATGGG TGATCCGTCG GGCCAGTGAG GAAGAGAAGC TGCTGTGCCT AGTGCGCCAG 
CGTGCAGGTC ACCGCTGTCA GAGCGCAGTA GTGGTAATCC TCATCCTGGC CTGGGAGGGG 
ATTCCACGCA ACATGGCCGA CAGACTGTAC CAGGAGCTTA CCCAGACCCT CTATAAATAT 
GGCTCGCCCA CCAGCCGCCG CTGCGCCCTT AATGAAGATC GTACCTGTGC CTGTCAGGGG 
CTGGACCCAG ATACCTGTGG AGCGTCCTTC TCTTTCGGCT GCTCGTGGAG TATGTATTTT 
AACGGCTGCA AGTTTGCCCG GAGCAAAGTG CCTAGAAAGT TCCGGCTGCA AGGAGACTAC 
CCAGAGGAGG AAGAAAAGAT CGAAAGCAAC CTCCTAAATC TGGCAACTGA TGTTGCACCT 
TTGTATAAGA AACTTGCTCC TGAAGCCTAT GAAAACCAGG TGGAGCAGGA GCAGTCAGGG 
CAAAACTGTC GGCTGGGCAG GAGAGAGGGT CGGCCATTTT CTGGTGTGAC AGCATGTGTG 
GACTTTTGTG CTCATGCTCA CAGGGACACA CACAACATGC ACAACGGGAG TACTGTGGTA 
TGCACTTTAA CTAAGGAGGA TAACCGTGCT GTAAGAAACA TTCCAGAGGA TGAGCAGCTG 
CATGTCCTGC CTCTTTACAA GATCTCGGAG ACTGATGAGT TCGGTCGTGT GGAGGGTCAG 
TGGGCTAAGA TACAGACTGG AGCTCTGCAG GTCCTCTCTT CCTTCCCAAG AGAGGTGCGT 
CTGCAGGCAG AGCCCGTCAA GTCTGCCCGT AAAAGGAGAC AAGAGGCCAA GAGAGAGCGA 
GAGAAGCAGA GAAACCAGGA GAAGAAGCAG GTCACACCAG TGAAAGTGAA GAACGAGCCC 
CTCAAAGGTT TTAAAAACTC TTCCTCTGAG CTTTCACCGT GTTTCAAAAC AGAAGCATGT 
TCTCCAACTC CTTATTGTTC CCCAATGCGA CTACCTAGGA CTCCAGACAA GGGGAGCCAC 
TTACTGGACT CGGCCAGTCC TTACAGGAGC ACATCATGTA CTCCTCCATT AAGCTCTACC 
AGAGACACAG CAGCACTTTC GTCCTCCCCT CACACCGTCC TCCCTGTGCC ACAATATCAG 
AATGTTGGAG AACTGGAGCA GAGCAATGAA TCTTCCCTCC TCAGACAGAG ACCAGGGTTA 
GAAGCAGCAA GACACAGCTC CCCTGAGCTC TCAGAACAGA AACCATTCAG AAACCAGCAG 
CAGATGGGCG GATCTTTGGG TTTCCGTGAC AAATGTTTTG CAGTAAAACC GGAGCCTGAG 
GAGGTCCGTT GTTTTCAAGG AGGAGCTGCT CCCCGACTGC TCTCCCCTTC CCGGGCTGAG 
GGTCTCCACA GCCGCCTGAA CTTTCGCCAA GCACAGCCGA GCCTGGATGA CCACCACGGG 
CCGCCGGTCA CCCCAAGGCC CCTGACCCCT GAGGAGGTCA AGGCAGAAGA GGTGTGGTCA 
GACAGCGAGC ACAACTTCCT AGATGGAGAC ATAGGCGGGG TCGCGGTGGC CCCCTCTCAC 
GGCTCCATCC TTATCGAGTG TGCCCGGCGG GAGCTGCACG CTACCACTCC CATTCTGCGG 
CCCAACCGCA GCCATCCCAC CCGAATCTCT CTGGTCTTCT ACCAGCACAA GAGCCTTAAC 
GTGCCTGGTC ACGGGCTGCT CCGGTGGGAA GCCAAGATGG CGGAGAAAGC CAGGGAGAAA 
GAGGAGGAGG CTATGCGGCT GGGACTAGAG ACGGAAGGTC TTGGGTCAGC TAAGACCAGA 
AAATCACAGC CGGCAGCGGG GAGCGACACG GAAAGCGGTG AGGAGGATGT GTGGCAGGAC 
GAGAGGGAGA AGCTACAGGT CCCTACCCGT CAGTCACTAA CAGCTACTCG GGACGGCCTC 
GTCACATCAG CCCCCTACTC CCTTACCCGG GTCACCGGGC CTTACAACCG CTGGATGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_007240335.1
CDS	457..6216
Translation	MPRPPKPQRKVHPPAKRNVRKLRASTDKKRVVNSNKPKGPATRTRQAKSRKSTAKKQATKKLQAGAADSKSAVKASQERASRSKNTSNSTESVRISRHTCNSLSQHDPAADLRSRTRSFRGSLLFQTFPQEVKPTRRAPSSRGRGQQAREQDNFKITECNDPNSDDQQKKALATNGEAQLVNRNSDETAEEEHREPLVHETPAVVDEEEVAVAAVTAETVQSENSLAFIDNTEQPSTTNESDDAFVKEHDPAVKVDYVCETDTSGPTDPPPSDLQSCDVAIRSTVDDSSQPDVSVASPVCEIPKESPALLEATDVKYAENGELDSTDDLKTKDENYDNDNDEFASKNEGALSLLSLSTGAYSGFLSTLDCGPSTTASLDSSKVSSSTDSTRFSFDTESEAGSLELALEHRAAGASPLLDVDIRQRLLQAQVRKERKKRSRCGACGPCLLKYSCGQCSCCLNRKTGHQICKLRKCVMLKKRPWQVTAGEVDSEGILVNGTNSEQMEETCVVPEEEARDVSQAHSAPPDSPTPLGHDTEPAPAERALQPAVSHHALLTHQSSLSSHNGANHMEPNTEDHRDPTDLQSKSQRTVNMPTKSQTPEAIPAQTVPLKKIKLEEPSTVSDQHSTSYLSSSDFYQDALSTLANVVCSTISDLKGLEEKTVGPQTSNACNIKTEREEEPYQSHSHQERTSNSPAGADNSHPKDPVPLTNNSVQSLVEHRNISIDQAIAIEALTQLAATPKSAPVKADSKVQDPPQESCNKLANVSSSRQSPVQEAKPISEVISNKVSVINSHQTSVICSPLNRQENPTHPKLSLQDLLIASSYDKLLHSPENGRVSQALCKTETLDGTFKTFKHVERTQTSRRRDEEEVAAQLVQLAFIIQSRTNPMSSENSPPKGMPVQTIKYNHHVVGQNVKKQRKPRTTPSSPRISKKKASEVEGGNHRIPLAKRTPNSKPPFKTKAQHVALQEKPKPQPKWSPFLPQTQIDLKKYLAQAHHEKHQLFHFSSKRQELDMQTLKSSGNQKAFTYKTDHAALSHSHVNQFRSNGHCHSLTNGHLGATHGQKYECEEHLISQVSKTYSVLNHGANPQAQYAMPSVPGADPLPGSPGRCHKVNSLSCEPRQTSFGQNGRYKVDTAGAVTVLSVSAGNMENANTESVEENTPTKHMLNSFLESPLKYLDTPTKNLINTPSKKTPELPSCDCMEQIIEKEEGPFYTHLGSGPTVAAVREMMEDRYGEKGKAVRVEVVVYTGREGRSSQGCPIAKWVIRRASEEEKLLCLVRQRAGHRCQSAVVVILILAWEGIPRNMADRLYQELTQTLYKYGSPTSRRCALNEDRTCACQGLDPDTCGASFSFGCSWSMYFNGCKFARSKVPRKFRLQGDYPEEEEKIESNLLNLATDVAPLYKKLAPEAYENQVEQEQSGQNCRLGRREGRPFSGVTACVDFCAHAHRDTHNMHNGSTVVCTLTKEDNRAVRNIPEDEQLHVLPLYKISETDEFGRVEGQWAKIQTGALQVLSSFPREVRLQAEPVKSARKRRQEAKREREKQRNQEKKQVTPVKVKNEPLKGFKNSSSELSPCFKTEACSPTPYCSPMRLPRTPDKGSHLLDSASPYRSTSCTPPLSSTRDTAALSSSPHTVLPVPQYQNVGELEQSNESSLLRQRPGLEAARHSSPELSEQKPFRNQQQMGGSLGFRDKCFAVKPEPEEVRCFQGGAAPRLLSPSRAEGLHSRLNFRQAQPSLDDHHGPPVTPRPLTPEEVKAEEVWSDSEHNFLDGDIGGVAVAPSHGSILIECARRELHATTPILRPNRSHPTRISLVFYQHKSLNVPGHGLLRWEAKMAEKAREKEEEAMRLGLETEGLGSAKTRKSQPAAGSDTESGEEDVWQDEREKLQVPTRQSLTATRDGLVTSAPYSLTRVTGPYNRWM

Target ORF information:

RefSeq Version	XM_007240273.2
Organism	Astyanax mexicanus(Mexican tetra)
Definition	Astyanax mexicanus methylcytosine dioxygenase TET3-like (LOC103033153), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_007240273.2

ORF Insert Sequence:

ATGCCTCGTC CTCCCAAACC ACAAAGGAAG GTGCATCCCC CAGCTAAGCG AAATGTGAGG 
AAGTTGAGGG CGTCCACTGA TAAAAAGCGA GTAGTAAATT CAAACAAGCC CAAGGGTCCA 
GCAACACGTA CAAGACAGGC GAAGAGCAGG AAATCCACAG CCAAGAAACA GGCAACCAAG 
AAGCTCCAGG CAGGTGCTGC TGATAGCAAG TCTGCCGTAA AGGCATCCCA GGAAAGAGCA 
AGCAGAAGCA AGAACACATC TAACTCAACA GAATCTGTGA GAATCTCCAG ACACACGTGT 
AACTCTCTGT CACAGCATGA CCCGGCTGCA GACCTCAGGA GCAGAACGAG GTCTTTTCGA 
GGCAGCCTGT TGTTCCAGAC TTTCCCACAG GAAGTAAAGC CAACAAGGAG GGCGCCCTCA 
AGCAGAGGGA GAGGTCAGCA GGCAAGGGAA CAGGATAATT TCAAAATCAC AGAATGTAAT 
GACCCTAATA GTGATGATCA ACAAAAGAAA GCACTTGCTA CCAATGGAGA GGCTCAACTT 
GTTAACAGAA ACTCTGATGA AACAGCAGAG GAGGAGCACA GAGAACCTCT TGTTCACGAG 
ACTCCTGCGG TGGTGGATGA GGAGGAGGTG GCTGTGGCTG CGGTCACTGC AGAAACAGTG 
CAAAGTGAAA ACAGCCTAGC CTTCATTGAT AACACTGAAC AACCAAGCAC AACTAATGAG 
TCAGATGACG CATTTGTGAA AGAGCATGAC CCAGCTGTTA AAGTGGACTA CGTTTGTGAA 
ACTGATACCT CAGGCCCAAC AGATCCTCCT CCTTCAGACC TTCAGTCTTG TGACGTGGCT 
ATTCGAAGCA CTGTAGACGA TTCTTCTCAA CCAGATGTGT CTGTGGCATC TCCCGTATGT 
GAAATTCCCA AAGAATCACC AGCACTTCTG GAAGCTACAG ATGTGAAGTA TGCTGAGAAC 
GGAGAGCTGG ACAGCACTGA CGACTTAAAG ACCAAAGATG AGAATTATGA CAATGACAAT 
GATGAGTTTG CAAGCAAGAA CGAAGGAGCA CTTTCACTCC TCTCACTGAG CACTGGTGCT 
TATAGTGGCT TTCTGTCCAC TCTCGACTGT GGGCCTTCAA CAACAGCATC ACTTGACAGC 
AGCAAAGTGT CCAGCAGCAC AGATAGCACC CGATTTTCAT TTGATACCGA GTCTGAAGCA 
GGGAGTTTAG AGCTAGCTCT AGAGCACAGA GCTGCTGGTG CTTCTCCTCT GCTGGATGTG 
GATATAAGGC AGCGCTTACT GCAAGCTCAG GTGAGAAAAG AGAGGAAGAA GAGGAGTAGA 
TGTGGGGCCT GCGGACCCTG CCTCCTCAAG TACAGCTGTG GGCAGTGCAG CTGCTGCCTA 
AATCGAAAGA CTGGCCATCA GATCTGTAAG CTCAGAAAAT GTGTGATGCT GAAGAAAAGA 
CCCTGGCAAG TTACAGCTGG AGAGGTTGAC TCAGAGGGCA TCTTAGTAAA TGGCACTAAT 
TCAGAGCAGA TGGAAGAGAC CTGTGTGGTG CCAGAGGAAG AGGCGCGTGA CGTATCTCAA 
GCACACTCCG CTCCACCTGA CTCTCCCACG CCTCTCGGGC ACGACACTGA ACCAGCACCA 
GCCGAAAGAG CCCTTCAGCC TGCTGTCAGC CACCATGCAC TTCTTACTCA CCAGAGCTCT 
TTAAGCTCCC ACAATGGTGC TAATCATATG GAACCAAACA CTGAGGATCA CAGAGATCCC 
ACTGACCTGC AGTCTAAGTC TCAAAGGACT GTGAACATGC CCACCAAAAG CCAGACGCCT 
GAGGCAATTC CTGCACAGAC AGTTCCTCTG AAGAAGATCA AGCTAGAAGA GCCTAGCACA 
GTGTCAGATC AACACAGCAC ATCCTACCTA AGCAGCAGCG ACTTCTACCA GGATGCTTTG 
TCTACTTTAG CAAATGTTGT ATGTTCAACC ATCTCTGATC TGAAGGGTTT GGAGGAGAAG 
ACAGTTGGTC CTCAGACCTC AAATGCCTGT AATATTAAAA CTGAAAGAGA AGAAGAACCA 
TATCAGAGTC ATTCACATCA GGAAAGGACT TCTAACAGTC CAGCAGGTGC TGATAACTCC 
CATCCGAAAG ACCCAGTTCC GCTGACTAAT AATAGTGTCC AGTCTTTAGT TGAGCATAGG 
AACATAAGCA TTGATCAGGC AATAGCTATT GAGGCCTTGA CCCAGCTGGC AGCTACCCCA 
AAATCAGCTC CTGTCAAGGC AGATTCTAAA GTTCAGGACC CTCCACAAGA ATCTTGTAAC 
AAATTAGCAA ATGTTTCATC AAGCAGGCAA AGCCCGGTCC AAGAGGCTAA ACCCATATCT 
GAGGTCATCT CCAATAAAGT CTCTGTAATC AATTCACATC AGACTTCTGT AATTTGCAGC 
CCTCTGAACA GACAGGAAAA CCCCACCCAC CCCAAACTCT CCTTACAGGA TCTTTTAATA 
GCTAGCTCAT ATGATAAGCT CTTGCATAGT CCAGAGAATG GACGAGTAAG TCAAGCACTT 
TGTAAAACAG AAACGTTAGA TGGCACTTTT AAGACTTTTA AGCATGTTGA AAGGACGCAG 
ACCTCAAGGA GGAGAGATGA GGAGGAAGTT GCAGCTCAGT TGGTGCAACT TGCATTCATA 
ATTCAATCAA GAACCAATCC CATGTCTTCT GAGAACAGTC CCCCCAAAGG GATGCCAGTA 
CAGACCATAA AATACAACCA TCATGTCGTT GGACAAAATG TAAAAAAGCA ACGGAAACCA 
AGAACAACTC CCTCAAGTCC CAGGATATCA AAGAAAAAAG CCTCTGAGGT TGAGGGAGGC 
AACCATAGGA TACCACTAGC CAAAAGGACA CCCAATAGCA AACCCCCTTT CAAGACAAAA 
GCCCAGCATG TGGCCTTGCA GGAAAAGCCC AAACCACAAC CCAAGTGGAG CCCGTTTCTC 
CCACAGACTC AGATAGACCT GAAAAAATAC CTAGCACAGG CTCATCATGA GAAACATCAG 
CTGTTCCATT TTAGCAGCAA GAGACAAGAG TTGGACATGC AGACTCTCAA GAGTTCCGGA 
AATCAGAAAG CTTTTACTTA CAAAACAGAC CACGCAGCTC TGAGTCATTC CCATGTTAAC 
CAATTCAGAT CCAACGGACA TTGTCACAGT CTCACAAATG GTCATCTAGG TGCTACACAT 
GGCCAGAAGT ATGAATGTGA AGAACATTTG ATTTCTCAGG TATCGAAAAC ATACAGTGTG 
CTAAATCATG GTGCTAATCC ACAAGCCCAG TATGCCATGC CTAGTGTCCC TGGTGCAGAT 
CCATTGCCAG GGAGTCCAGG GAGGTGCCAT AAAGTAAACA GCCTAAGCTG TGAGCCGCGG 
CAGACTTCCT TTGGTCAGAA TGGACGTTAC AAAGTGGACA CGGCTGGCGC TGTCACCGTT 
TTGTCTGTGT CAGCTGGAAA CATGGAAAAT GCTAACACTG AATCCGTTGA AGAAAACACT 
CCCACCAAGC ATATGCTTAA CAGCTTTTTG GAATCTCCGT TGAAGTACTT GGACACTCCA 
ACCAAGAATC TAATCAACAC ACCCTCCAAA AAAACTCCGG AGCTCCCCTC CTGTGACTGT 
ATGGAGCAAA TCATTGAAAA AGAGGAGGGT CCATTCTACA CACACCTTGG CTCTGGGCCA 
ACTGTAGCAG CAGTCCGAGA GATGATGGAG GACAGGTATG GGGAGAAGGG CAAAGCAGTG 
CGTGTGGAAG TTGTAGTTTA TACTGGGAGA GAAGGAAGGA GCTCCCAGGG CTGTCCAATA 
GCCAAATGGG TGATCCGTCG GGCCAGTGAG GAAGAGAAGC TGCTGTGCCT AGTGCGCCAG 
CGTGCAGGTC ACCGCTGTCA GAGCGCAGTA GTGGTAATCC TCATCCTGGC CTGGGAGGGG 
ATTCCACGCA ACATGGCCGA CAGACTGTAC CAGGAGCTTA CCCAGACCCT CTATAAATAT 
GGCTCGCCCA CCAGCCGCCG CTGCGCCCTT AATGAAGATC GTACCTGTGC CTGTCAGGGG 
CTGGACCCAG ATACCTGTGG AGCGTCCTTC TCTTTCGGCT GCTCGTGGAG TATGTATTTT 
AACGGCTGCA AGTTTGCCCG GAGCAAAGTG CCTAGAAAGT TCCGGCTGCA AGGAGACTAC 
CCAGAGGAGG AAGAAAAGAT CGAAAGCAAC CTCCTAAATC TGGCAACTGA TGTTGCACCT 
TTGTATAAGA AACTTGCTCC TGAAGCCTAT GAAAACCAGG TGGAGCAGGA GCAGTCAGGG 
CAAAACTGTC GGCTGGGCAG GAGAGAGGGT CGGCCATTTT CTGGTGTGAC AGCATGTGTG 
GACTTTTGTG CTCATGCTCA CAGGGACACA CACAACATGC ACAACGGGAG TACTGTGGTA 
TGCACTTTAA CTAAGGAGGA TAACCGTGCT GTAAGAAACA TTCCAGAGGA TGAGCAGCTG 
CATGTCCTGC CTCTTTACAA GATCTCGGAG ACTGATGAGT TCGGTCGTGT GGAGGGTCAG 
TGGGCTAAGA TACAGACTGG AGCTCTGCAG GTCCTCTCTT CCTTCCCAAG AGAGGTGCGT 
CTGCAGGCAG AGCCCGTCAA GTCTGCCCGT AAAAGGAGAC AAGAGGCCAA GAGAGAGCGA 
GAGAAGCAGA GAAACCAGGA GAAGAAGCAG GTCACACCAG TGAAAGTGAA GAACGAGCCC 
CTCAAAGGTT TTAAAAACTC TTCCTCTGAG CTTTCACCGT GTTTCAAAAC AGAAGCATGT 
TCTCCAACTC CTTATTGTTC CCCAATGCGA CTACCTAGGA CTCCAGACAA GGGGAGCCAC 
TTACTGGACT CGGCCAGTCC TTACAGGAGC ACATCATGTA CTCCTCCATT AAGCTCTACC 
AGAGACACAG CAGCACTTTC GTCCTCCCCT CACACCGTCC TCCCTGTGCC ACAATATCAG 
AATGTTGGAG AACTGGAGCA GAGCAATGAA TCTTCCCTCC TCAGACAGAG ACCAGGGTTA 
GAAGCAGCAA GACACAGCTC CCCTGAGCTC TCAGAACAGA AACCATTCAG AAACCAGCAG 
CAGATGGGCG GATCTTTGGG TTTCCGTGAC AAATGTTTTG CAGTAAAACC GGAGCCTGAG 
GAGGTCCGTT GTTTTCAAGG AGGAGCTGCT CCCCGACTGC TCTCCCCTTC CCGGGCTGAG 
GGTCTCCACA GCCGCCTGAA CTTTCGCCAA GCACAGCCGA GCCTGGATGA CCACCACGGG 
CCGCCGGTCA CCCCAAGGCC CCTGACCCCT GAGGAGGTCA AGGCAGAAGA GGTGTGGTCA 
GACAGCGAGC ACAACTTCCT AGATGGAGAC ATAGGCGGGG TCGCGGTGGC CCCCTCTCAC 
GGCTCCATCC TTATCGAGTG TGCCCGGCGG GAGCTGCACG CTACCACTCC CATTCTGCGG 
CCCAACCGCA GCCATCCCAC CCGAATCTCT CTGGTCTTCT ACCAGCACAA GAGCCTTAAC 
GTGCCTGGTC ACGGGCTGCT CCGGTGGGAA GCCAAGATGG CGGAGAAAGC CAGGGAGAAA 
GAGGAGGAGG CTATGCGGCT GGGACTAGAG ACGGAAGGTC TTGGGTCAGC TAAGACCAGA 
AAATCACAGC CGGCAGCGGG GAGCGACACG GAAAGCGGTG AGGAGGATGT GTGGCAGGAC 
GAGAGGGAGA AGCTACAGGT CCCTACCCGT CAGTCACTAA CAGCTACTCG GGACGGCCTC 
GTCACATCAG CCCCCTACTC CCTTACCCGG GTCACCGGGC CTTACAACCG CTGGATGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

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1-877-436-7274

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LOC103033153 cDNA ORF clone, Astyanax mexicanus(Mexican tetra)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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