PPO cDNA ORF clone, Apis mellifera(honey bee)

Gene
Clones
gRNAs

Summary

Gene Symbol	PPO
Entrez Gene ID	406155
Full Name	phenoloxidase subunit A3
Synonyms	GB18313,ppoq
Gene Type	protein-coding
Organism	Apis mellifera(honey bee)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001011627.1	NP_001011627.1	phenoloxidase subunit A3

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Finding the missing honey bee genes: lessons learned from a genome upgrade.
Elsik CG, Worley KC, Bennett AK, Beye M, Camara F, Childers CP, de Graaf DC, Debyser G, Deng J, Devreese B, Elhaik E, Evans JD, Foster LJ, Graur D, Guigo R, , Hoff KJ, Holder ME, Hudson ME, Hunt GJ, Jiang H, Joshi V, Khetani RS, Kosarev P, Kovar CL, Ma J, Maleszka R, Moritz RF, Munoz-Torres MC, Murphy TD, Muzny DM, Newsham IF, Reese JT, Robertson HM, Robinson GE, Rueppell O, Solovyev V, Stanke M, Stolle E, Tsuruda JM, Vaerenbergh MV, Waterhouse RM, Weaver DB, Whitfield CW, Wu Y, Zdobnov EM, Zhang L, Zhu D, Gibbs RA,
BMC genomics1586(2014 Jan)

Organization, evolution and transcriptional profile of hexamerin genes of the parasitic wasp Nasonia vitripennis (Hymenoptera: Pteromalidae).
Cristino AS, Nunes FM, Barchuk AR, Aguiar-Coelho VM, Simões ZL, Bitondi MM
Insect molecular biology19 Suppl 1137-46(2010 Feb)

Insights into social insects from the genome of the honeybee Apis mellifera.

Nature443(7114)931-49(2006 Oct)

Molecular characterization of a cDNA encoding prophenoloxidase and its expression in Apis mellifera.
Lourenço AP, Zufelato MS, Bitondi MM, Simões ZL
Insect biochemistry and molecular biology35(6)541-52(2005 Jun)

Phenoloxidase activity in Apis mellifera honey bee pupae, and ecdysteroid-dependent expression of the prophenoloxidase mRNA.
Zufelato MS, Lourenço AP, Simões ZL, Jorge JA, Bitondi MM
Insect biochemistry and molecular biology34(12)1257-68(2004 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following PPO gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the PPO cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OHd00182	NM_001011627.1 Latest version!	Apis mellifera phenoloxidase subunit A3 (PPO), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

PPO ( NM_001011627.1 ) cDNA ORF clone, Apis mellifera(honey bee) -> NP_001011627.1 Apis mellifera phenoloxidase subunit A3 (PPO), mRNA.

Price & Availability↑

CloneID	OHd00182
Clone ID Related Accession (Same CDS sequence)	NM_001011627.1
Accession Version	NM_001011627.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2082bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1556812800000
Organism	Apis mellifera(honey bee)
Product	phenoloxidase subunit A3
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AY242387.2. On Feb 5, 2005 this sequence version replaced XM_392817.1. ##Evidence-Data-START## Transcript exon combination :: AY242387.2 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00008880, SAMN00120367 [ECO:0000348] ##Evidence-Data-END##

ATGGCGTCTG ATAAATCGGG TATTTTGTAT CTATTTGATC GACCTTCTGA ACCAGTTTAC 
GTCCCTAAGG GAGACAATAA AGTCGCTTTT GATATTCCAC CTGATTATTT GCCTGATAGA 
TATCGTTCTG TGGCTACTCA AGTATTTAAT CGTTTCGGCG ATGATACGGA ATCGAAACTT 
CCAGTGAAGG CAATTACTCT TCCGGATCTT AGTATTCCAA TGCAACTTGG ACGTCGTCAA 
CCTTTTTCAT TATTTATCCC GGCACATCGA AAAATAGCTG CTCGATTAAT TGATATTTTT 
ATGGGTATGC GAACATATGA GGACTTCCTG TCTGTTGCAG TTTATTGTCG CGATCGTTTA 
AATCCAAATT TGTTTATTTA TGCCCTATCA GTGGCGATAC TTCATCGTCC TGACACGAAA 
GATTTGCCAG TACCACCGCT TACCGAAGTA TTTCCAGACA AATATATGGA TAGTGGAATA 
TTTTCCAGGG CTAGAGAAGA AGCTAATGTT GTACCAGAGG GTGCTAGAGT TCCAATTGAA 
ATTCCAAGAG ATTATACCGC ATCAGATCTT GATGTGGAAC ATCGTGTTGC TTATTGGAGA 
GAAGATATTG GAATTAATCT TCATCATTGG CATTGGCACT TGGTGTATCC ATTTGAAGGT 
GACATTAGAA TTGTTAACAA GGATCGACGT GGAGAACTTT TCTATTATAT GCATCAACAG 
ATTATGGCTA GATATAATTG CGAACGGCTA TGTAATCGTC TTGGACGAGT AAAACGATTC 
ATTAATTGGC ACGAACCGAT TCCAGAAGCT TACTTCCCGA AATTAGATTC CCTTGTTGCC 
AGTCGGACAT GGCCATTTCG ACCCAGCGGT ACTGTTCTTA AGGACATCAA TCGACAAGTT 
GATGAATTGA ACTTTGACAT TCAGGATCTA GAAAGATGGC GTGATCGTAT TTACGAGGCA 
ATCCATACAG GATCTGTGAT TAATACCAGG GGTGAAAGAA TTCAATTGAC TGAGAAAAAT 
GGAATCGACG TCCTGGGAAA TATCATGGAA GCTAGTATTC TTTCTCCCAA TCAGAATGTC 
TATGGTGATC TCCATAATTT TGGTCATGTC GCCATTTCTT ATATCCATGA TCCGGATCAT 
CGATATTTGG AGAGTTTTGG TGTTATGGGT GATTCAGCAA CAGCTATGAG AGATCCTATA 
TTTTATAGAT GGCATGCATT TGTTGATGAC GTGTTTCAGG AACATAAAAA TACATTACCT 
CAGTATACTG TACAACAGTT AGACTTCCCA GGTATCGAAA TAGCAGATAT CAAATTGACA 
ACCAATCAAC AACGCAATAT TTTAAATACC TTTTGGACCA AAAGTGATGT AGATTTGTCA 
CGCGGACTCG ATTTCACTCC ACGTGGCGCA GTGCTTGCTA GATTTACTCA CTTGAACCAT 
GCTGATTTTT CATATACGAT TGTTATTAAT AATCGTAATA ATACATCAAT GAAAGGCACG 
GTCCGTATCT TTATAGGTCC TAAAGAAGAC GAGCGTGGAT TACCTTTTAC TTTCAGAGAA 
CAGAAGAATT TAATGATCGA ATTGGATAAG TTTCCTATAA CACTTCAACC AGGAAAAAAT 
ACGATTGAAC AGAAATCTAC GAAATCTTCA GTAACTATAC CATTTGAGAG AACTTTCCGC 
AACTTAGATG AAAATAGACC AATAGGAGGT GATAGTTTGG AACGATTCGA TTTTTGTGGA 
TGTGGATGGC CGCAACATAT GCTTATACCA AAAGGAAATA AAGAAGGTTT TGCAATGGAA 
CTTTTCGTCA TGGTATCAGA TTATAAAGAT GATCGTGTTG AACAAAATGA GCCAATTGGT 
TGTAAGGATG CCTCAAGTTA TTGTGGATTA AGAGATAGAA AGTATCCTGA TGCACGAGCA 
ATGGGATATC CATTTGATCG TCAACCTCGT GCAGGTGTTG AAACTTTAGC TCAATTCCTC 
ACCGGAAATA TGGCGGTAAC TGAAGTAACA GTTCGATTTA GTGATACAAT TGTACCTAGA 
TCTAGATCTG GAAGTATAAG CAATACTTTA ACCTTTATGT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001011627.1
CDS	209..2290
Translation	MASDKSGILYLFDRPSEPVYVPKGDNKVAFDIPPDYLPDRYRSVATQVFNRFGDDTESKLPVKAITLPDLSIPMQLGRRQPFSLFIPAHRKIAARLIDIFMGMRTYEDFLSVAVYCRDRLNPNLFIYALSVAILHRPDTKDLPVPPLTEVFPDKYMDSGIFSRAREEANVVPEGARVPIEIPRDYTASDLDVEHRVAYWREDIGINLHHWHWHLVYPFEGDIRIVNKDRRGELFYYMHQQIMARYNCERLCNRLGRVKRFINWHEPIPEAYFPKLDSLVASRTWPFRPSGTVLKDINRQVDELNFDIQDLERWRDRIYEAIHTGSVINTRGERIQLTEKNGIDVLGNIMEASILSPNQNVYGDLHNFGHVAISYIHDPDHRYLESFGVMGDSATAMRDPIFYRWHAFVDDVFQEHKNTLPQYTVQQLDFPGIEIADIKLTTNQQRNILNTFWTKSDVDLSRGLDFTPRGAVLARFTHLNHADFSYTIVINNRNNTSMKGTVRIFIGPKEDERGLPFTFREQKNLMIELDKFPITLQPGKNTIEQKSTKSSVTIPFERTFRNLDENRPIGGDSLERFDFCGCGWPQHMLIPKGNKEGFAMELFVMVSDYKDDRVEQNEPIGCKDASSYCGLRDRKYPDARAMGYPFDRQPRAGVETLAQFLTGNMAVTEVTVRFSDTIVPRSRSGSISNTLTFM

Target ORF information:

RefSeq Version	NM_001011627.1
Organism	Apis mellifera(honey bee)
Definition	Apis mellifera phenoloxidase subunit A3 (PPO), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001011627.1

ORF Insert Sequence:

ATGGCGTCTG ATAAATCGGG TATTTTGTAT CTATTTGATC GACCTTCTGA ACCAGTTTAC 
GTCCCTAAGG GAGACAATAA AGTCGCTTTT GATATTCCAC CTGATTATTT GCCTGATAGA 
TATCGTTCTG TGGCTACTCA AGTATTTAAT CGTTTCGGCG ATGATACGGA ATCGAAACTT 
CCAGTGAAGG CAATTACTCT TCCGGATCTT AGTATTCCAA TGCAACTTGG ACGTCGTCAA 
CCTTTTTCAT TATTTATCCC GGCACATCGA AAAATAGCTG CTCGATTAAT TGATATTTTT 
ATGGGTATGC GAACATATGA GGACTTCCTG TCTGTTGCAG TTTATTGTCG CGATCGTTTA 
AATCCAAATT TGTTTATTTA TGCCCTATCA GTGGCGATAC TTCATCGTCC TGACACGAAA 
GATTTGCCAG TACCACCGCT TACCGAAGTA TTTCCAGACA AATATATGGA TAGTGGAATA 
TTTTCCAGGG CTAGAGAAGA AGCTAATGTT GTACCAGAGG GTGCTAGAGT TCCAATTGAA 
ATTCCAAGAG ATTATACCGC ATCAGATCTT GATGTGGAAC ATCGTGTTGC TTATTGGAGA 
GAAGATATTG GAATTAATCT TCATCATTGG CATTGGCACT TGGTGTATCC ATTTGAAGGT 
GACATTAGAA TTGTTAACAA GGATCGACGT GGAGAACTTT TCTATTATAT GCATCAACAG 
ATTATGGCTA GATATAATTG CGAACGGCTA TGTAATCGTC TTGGACGAGT AAAACGATTC 
ATTAATTGGC ACGAACCGAT TCCAGAAGCT TACTTCCCGA AATTAGATTC CCTTGTTGCC 
AGTCGGACAT GGCCATTTCG ACCCAGCGGT ACTGTTCTTA AGGACATCAA TCGACAAGTT 
GATGAATTGA ACTTTGACAT TCAGGATCTA GAAAGATGGC GTGATCGTAT TTACGAGGCA 
ATCCATACAG GATCTGTGAT TAATACCAGG GGTGAAAGAA TTCAATTGAC TGAGAAAAAT 
GGAATCGACG TCCTGGGAAA TATCATGGAA GCTAGTATTC TTTCTCCCAA TCAGAATGTC 
TATGGTGATC TCCATAATTT TGGTCATGTC GCCATTTCTT ATATCCATGA TCCGGATCAT 
CGATATTTGG AGAGTTTTGG TGTTATGGGT GATTCAGCAA CAGCTATGAG AGATCCTATA 
TTTTATAGAT GGCATGCATT TGTTGATGAC GTGTTTCAGG AACATAAAAA TACATTACCT 
CAGTATACTG TACAACAGTT AGACTTCCCA GGTATCGAAA TAGCAGATAT CAAATTGACA 
ACCAATCAAC AACGCAATAT TTTAAATACC TTTTGGACCA AAAGTGATGT AGATTTGTCA 
CGCGGACTCG ATTTCACTCC ACGTGGCGCA GTGCTTGCTA GATTTACTCA CTTGAACCAT 
GCTGATTTTT CATATACGAT TGTTATTAAT AATCGTAATA ATACATCAAT GAAAGGCACG 
GTCCGTATCT TTATAGGTCC TAAAGAAGAC GAGCGTGGAT TACCTTTTAC TTTCAGAGAA 
CAGAAGAATT TAATGATCGA ATTGGATAAG TTTCCTATAA CACTTCAACC AGGAAAAAAT 
ACGATTGAAC AGAAATCTAC GAAATCTTCA GTAACTATAC CATTTGAGAG AACTTTCCGC 
AACTTAGATG AAAATAGACC AATAGGAGGT GATAGTTTGG AACGATTCGA TTTTTGTGGA 
TGTGGATGGC CGCAACATAT GCTTATACCA AAAGGAAATA AAGAAGGTTT TGCAATGGAA 
CTTTTCGTCA TGGTATCAGA TTATAAAGAT GATCGTGTTG AACAAAATGA GCCAATTGGT 
TGTAAGGATG CCTCAAGTTA TTGTGGATTA AGAGATAGAA AGTATCCTGA TGCACGAGCA 
ATGGGATATC CATTTGATCG TCAACCTCGT GCAGGTGTTG AAACTTTAGC TCAATTCCTC 
ACCGGAAATA TGGCGGTAAC TGAAGTAACA GTTCGATTTA GTGATACAAT TGTACCTAGA 
TCTAGATCTG GAAGTATAAG CAATACTTTA ACCTTTATGT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Finding the missing honey bee genes: lessons learned from a genome upgrade.
BMC genomics1586(2014 Jan)
Elsik CG,Worley KC,Bennett AK,Beye M,Camara F,Childers CP,de Graaf DC,Debyser G,Deng J,Devreese B,Elhaik E,Evans JD,Foster LJ,Graur D,Guigo R,,Hoff KJ,Holder ME,Hudson ME,Hunt GJ,Jiang H,Joshi V,Khetani RS,Kosarev P,Kovar CL,Ma J,Maleszka R,Moritz RF,Munoz-Torres MC,Murphy TD,Muzny DM,Newsham IF,Reese JT,Robertson HM,Robinson GE,Rueppell O,Solovyev V,Stanke M,Stolle E,Tsuruda JM,Vaerenbergh MV,Waterhouse RM,Weaver DB,Whitfield CW,Wu Y,Zdobnov EM,Zhang L,Zhu D,Gibbs RA,

Organization, evolution and transcriptional profile of hexamerin genes of the parasitic wasp Nasonia vitripennis (Hymenoptera: Pteromalidae).
Insect molecular biology19 Suppl 1137-46(2010 Feb)
Cristino AS,Nunes FM,Barchuk AR,Aguiar-Coelho VM,Simões ZL,Bitondi MM

Insights into social insects from the genome of the honeybee Apis mellifera.
Nature443(7114)931-49(2006 Oct)

Molecular characterization of a cDNA encoding prophenoloxidase and its expression in Apis mellifera.
Insect biochemistry and molecular biology35(6)541-52(2005 Jun)
Lourenço AP,Zufelato MS,Bitondi MM,Simões ZL

Phenoloxidase activity in Apis mellifera honey bee pupae, and ecdysteroid-dependent expression of the prophenoloxidase mRNA.
Insect biochemistry and molecular biology34(12)1257-68(2004 Dec)
Zufelato MS,Lourenço AP,Simões ZL,Jorge JA,Bitondi MM