DGKA cDNA ORF clone, Ailuropoda melanoleuca(giant panda)

Gene
Clones
gRNAs

Summary

Gene Symbol	DGKA
Entrez Gene ID	100467810
Full Name	diacylglycerol kinase alpha
Gene Type	protein-coding
Organism	Ailuropoda melanoleuca(giant panda)

mRNA and Protein(s)

mRNA	Protein	Name
XM_002916054.3	XP_002916100.1	diacylglycerol kinase alpha isoform X1
XM_019796140.1	XP_019651699.1	diacylglycerol kinase alpha isoform X1
XM_019796141.1	XP_019651700.1	diacylglycerol kinase alpha isoform X2

Pathways from BioSystems
Gene Ontology

The following DGKA gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the DGKA cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OAc03610	XM_002916054.3 Latest version!	Ailuropoda melanoleuca diacylglycerol kinase alpha (DGKA), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
OAc03610	XM_019796140.1 Latest version!	Ailuropoda melanoleuca diacylglycerol kinase alpha (DGKA), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
OAc90502	XM_019796141.1 Latest version!	Ailuropoda melanoleuca diacylglycerol kinase alpha (DGKA), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

DGKA ( XM_002916054.3 ) cDNA ORF clone, Ailuropoda melanoleuca(giant panda) -> XP_002916100.1 Ailuropoda melanoleuca diacylglycerol kinase alpha (DGKA), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OAc03610
Clone ID Related Accession (Same CDS sequence)	XM_002916054.3 , XM_019796140.1
Accession Version	XM_002916054.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2202bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1482768000000
Organism	Ailuropoda melanoleuca(giant panda)
Product	diacylglycerol kinase alpha isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003217407.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Dec 28, 2016 this sequence version replaced XM_002916054.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Ailuropoda melanoleuca Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCAAGG AGAGGGGCCT AATAAGCCCC AATGATTTTG CCCAGCTGCA AAAGTACATG 
GAATACTCCA CCAACAAGGT CAGCGATGTC CTAAAGCTCT TTGAGGATGG TGAAATGGCT 
GAATATCTCC AAGGAGATGC CATTGGGTAC GAGGGATTCC AGCAATTTTT GAAAATCTAT 
CTGGAAGCAG ATAACGTTCC CAATCACCTA AGCCTGGCAC TGTTTCAGTC CTTCAAGATG 
GGTCACTTTG AAGACACTGT GAAAAATGAT GTGGTGTATC TCAGTGATGT CTCCTGCTAC 
TTTTCCCTTC TGGAGGGTGG CCGGCCAGAA GACAAGCTAG AATTCACCTT CAAGCTGTAC 
GACACGGACA GAAATGGGAT CCTGGACAGC TCAGAAGTGG ACAGAATCAT CATACAGATG 
ATGCGAGTGG CTGAATACCT GGACTGGGAT GTGTCTGAGC TGAGGCCGAT TCTCCAGGAG 
ATGATGAAAG AGATTGACTA TGATGGCAGT GGCTCTGTCT CCCTCGCTGA GTGGCTCCGG 
GCTGGGGCGA CCACCGTGCC GCTGCTAGTG CTGCTGGGTC TGGAGATGAC TCTGAAGGAC 
AATGGGCAGC ACCTGTGGAG ACCCAAGAGG TTCCCCCGAC CGGTCTACTG CAATCTGTGC 
GAGTCGAGCA TTGGTCTTGG CAAACAGGGG CTGAGCTGTA ACCTCTGTAA GTACGTCGTT 
CACGACCAGT GTGCCATGAA GGCCTTGCCT TGTGAGGTCA GCACGTACGC CAAGTCTCGG 
AAGGACATCG GTATCCAATC GCATGTGTGG GTGAGAGGAG GCTGTGAGTC CGGCCGCTGC 
GACCGCTGTC AGAAGAAGAT CCGCATTTAC CACAGTCTCG TTGGACTGCA TTGTGTGTGG 
TGCCACCTAG AGATTCATGA TGACTGCCTA CAAGCCATGG GTCATGAATG TGACTGTGGG 
CTGCTCCGGG ATCATATCCT GCCTCCATCT TCCATCTATC CCAGTGTCCT GGCCTCTGGA 
CAGGAGCGTA AAATTAGCAA AACAAGCCAG AAGACCGTGG ATGACTTAAA TTTGAGCACC 
TTTGAGGCTC TGCGGATTGA CCCTGTTTAT AACACCCACC CACTTCTGGT CTTTGTCAAT 
CCTAAGAGTG GCGGGAAGCA GGGGGAGAGA GTGCTTTGGA AATTCCAGTA TCTACTAAAT 
CCTCGACAGG TATTCAACCT CCTAAAGGAT GGTCCTGAGC CAGGGCTCAG ATTCTTCAGG 
GATGTTCCCG GTTGCCGGAT TTTGGTGTGT GGTGGAGATG GCACAGTAGG CTGGATTCTA 
GAGACCATAG ACAAAGCCAA CTTGCCTGTT GTGCCTCCTG TTGCTGTGTT GCCCCTGGGC 
ACTGGAAATG ATCTGGCTCG TTGCCTAAGA TGGGGAGGAG GATATGAGGG ACAAAATTTG 
GGGAAGATCC TCAAGGATTT AGAGATGAGT AAGGTGGTAC ATATGGATCG ATGGTCCGTG 
GAGGTGATAC CTCAACAAAC TGAAGAAAAG AGTGACCCAG TCCCCTTTCA AATCATCAAT 
AACTACTTCT CCATCGGTGT GGATGCCTCT ATTGCTCACC GATTCCACAT CATGCGAGAG 
AAATATCCTG AGAAATTCAA CAGCAGAATG AAGAACAAGC TATGGTACTT CGAATTCGCC 
ACTTCCGAAT CCATCTTCTC AACGTGCAAA AAGCTGGAGG AGTCTTTGAC AGTCGAGATC 
TGTGGGAAAC CACTGGATCT GAGCAACCTG TCCCTAGAAG GCATCGCAGT GCTGAACATC 
CCTAGCATGC ATGGTGGTTC CAACCTCTGG GGTGATACCA AGAGACCTCA TGGAGATACC 
CATGGGATCA ACCAGGCCTT AGGCGCTGCA GCTAAAGTCA TCACTGACCC GGATATCCTG 
AAAACCTGTG TACCAGACCT AAGTGACAAG CGGCTGGAAG TAGTCGGGCT GGAGGGTGCA 
ATTGAGATGG GCCAGATTTA TACCAAGCTC AAGAATGCTG GACATCGGCT GGCCAAGTGC 
TCTGAAATCA CCTTCCACAC CACAAAAACC CTCCCCATGC AAATCGACGG AGAGCCTTGG 
ATGCAGACAC CCTGTACAAT CAAGATCACC CACAAGAACC AGATGCCCAT GCTTGTGGGC 
CCACCCCCTC GCTCCTCCAA TTTCTTTGGC TTCTTATGCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_002916100.1
CDS	263..2464
Translation	MAKERGLISPNDFAQLQKYMEYSTNKVSDVLKLFEDGEMAEYLQGDAIGYEGFQQFLKIYLEADNVPNHLSLALFQSFKMGHFEDTVKNDVVYLSDVSCYFSLLEGGRPEDKLEFTFKLYDTDRNGILDSSEVDRIIIQMMRVAEYLDWDVSELRPILQEMMKEIDYDGSGSVSLAEWLRAGATTVPLLVLLGLEMTLKDNGQHLWRPKRFPRPVYCNLCESSIGLGKQGLSCNLCKYVVHDQCAMKALPCEVSTYAKSRKDIGIQSHVWVRGGCESGRCDRCQKKIRIYHSLVGLHCVWCHLEIHDDCLQAMGHECDCGLLRDHILPPSSIYPSVLASGQERKISKTSQKTVDDLNLSTFEALRIDPVYNTHPLLVFVNPKSGGKQGERVLWKFQYLLNPRQVFNLLKDGPEPGLRFFRDVPGCRILVCGGDGTVGWILETIDKANLPVVPPVAVLPLGTGNDLARCLRWGGGYEGQNLGKILKDLEMSKVVHMDRWSVEVIPQQTEEKSDPVPFQIINNYFSIGVDASIAHRFHIMREKYPEKFNSRMKNKLWYFEFATSESIFSTCKKLEESLTVEICGKPLDLSNLSLEGIAVLNIPSMHGGSNLWGDTKRPHGDTHGINQALGAAAKVITDPDILKTCVPDLSDKRLEVVGLEGAIEMGQIYTKLKNAGHRLAKCSEITFHTTKTLPMQIDGEPWMQTPCTIKITHKNQMPMLVGPPPRSSNFFGFLC

Target ORF information:

RefSeq Version	XM_002916054.3
Organism	Ailuropoda melanoleuca(giant panda)
Definition	Ailuropoda melanoleuca diacylglycerol kinase alpha (DGKA), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_002916054.3

ORF Insert Sequence:

ATGGCCAAGG AGAGGGGCCT AATAAGCCCC AATGATTTTG CCCAGCTGCA AAAGTACATG 
GAATACTCCA CCAACAAGGT CAGCGATGTC CTAAAGCTCT TTGAGGATGG TGAAATGGCT 
GAATATCTCC AAGGAGATGC CATTGGGTAC GAGGGATTCC AGCAATTTTT GAAAATCTAT 
CTGGAAGCAG ATAACGTTCC CAATCACCTA AGCCTGGCAC TGTTTCAGTC CTTCAAGATG 
GGTCACTTTG AAGACACTGT GAAAAATGAT GTGGTGTATC TCAGTGATGT CTCCTGCTAC 
TTTTCCCTTC TGGAGGGTGG CCGGCCAGAA GACAAGCTAG AATTCACCTT CAAGCTGTAC 
GACACGGACA GAAATGGGAT CCTGGACAGC TCAGAAGTGG ACAGAATCAT CATACAGATG 
ATGCGAGTGG CTGAATACCT GGACTGGGAT GTGTCTGAGC TGAGGCCGAT TCTCCAGGAG 
ATGATGAAAG AGATTGACTA TGATGGCAGT GGCTCTGTCT CCCTCGCTGA GTGGCTCCGG 
GCTGGGGCGA CCACCGTGCC GCTGCTAGTG CTGCTGGGTC TGGAGATGAC TCTGAAGGAC 
AATGGGCAGC ACCTGTGGAG ACCCAAGAGG TTCCCCCGAC CGGTCTACTG CAATCTGTGC 
GAGTCGAGCA TTGGTCTTGG CAAACAGGGG CTGAGCTGTA ACCTCTGTAA GTACGTCGTT 
CACGACCAGT GTGCCATGAA GGCCTTGCCT TGTGAGGTCA GCACGTACGC CAAGTCTCGG 
AAGGACATCG GTATCCAATC GCATGTGTGG GTGAGAGGAG GCTGTGAGTC CGGCCGCTGC 
GACCGCTGTC AGAAGAAGAT CCGCATTTAC CACAGTCTCG TTGGACTGCA TTGTGTGTGG 
TGCCACCTAG AGATTCATGA TGACTGCCTA CAAGCCATGG GTCATGAATG TGACTGTGGG 
CTGCTCCGGG ATCATATCCT GCCTCCATCT TCCATCTATC CCAGTGTCCT GGCCTCTGGA 
CAGGAGCGTA AAATTAGCAA AACAAGCCAG AAGACCGTGG ATGACTTAAA TTTGAGCACC 
TTTGAGGCTC TGCGGATTGA CCCTGTTTAT AACACCCACC CACTTCTGGT CTTTGTCAAT 
CCTAAGAGTG GCGGGAAGCA GGGGGAGAGA GTGCTTTGGA AATTCCAGTA TCTACTAAAT 
CCTCGACAGG TATTCAACCT CCTAAAGGAT GGTCCTGAGC CAGGGCTCAG ATTCTTCAGG 
GATGTTCCCG GTTGCCGGAT TTTGGTGTGT GGTGGAGATG GCACAGTAGG CTGGATTCTA 
GAGACCATAG ACAAAGCCAA CTTGCCTGTT GTGCCTCCTG TTGCTGTGTT GCCCCTGGGC 
ACTGGAAATG ATCTGGCTCG TTGCCTAAGA TGGGGAGGAG GATATGAGGG ACAAAATTTG 
GGGAAGATCC TCAAGGATTT AGAGATGAGT AAGGTGGTAC ATATGGATCG ATGGTCCGTG 
GAGGTGATAC CTCAACAAAC TGAAGAAAAG AGTGACCCAG TCCCCTTTCA AATCATCAAT 
AACTACTTCT CCATCGGTGT GGATGCCTCT ATTGCTCACC GATTCCACAT CATGCGAGAG 
AAATATCCTG AGAAATTCAA CAGCAGAATG AAGAACAAGC TATGGTACTT CGAATTCGCC 
ACTTCCGAAT CCATCTTCTC AACGTGCAAA AAGCTGGAGG AGTCTTTGAC AGTCGAGATC 
TGTGGGAAAC CACTGGATCT GAGCAACCTG TCCCTAGAAG GCATCGCAGT GCTGAACATC 
CCTAGCATGC ATGGTGGTTC CAACCTCTGG GGTGATACCA AGAGACCTCA TGGAGATACC 
CATGGGATCA ACCAGGCCTT AGGCGCTGCA GCTAAAGTCA TCACTGACCC GGATATCCTG 
AAAACCTGTG TACCAGACCT AAGTGACAAG CGGCTGGAAG TAGTCGGGCT GGAGGGTGCA 
ATTGAGATGG GCCAGATTTA TACCAAGCTC AAGAATGCTG GACATCGGCT GGCCAAGTGC 
TCTGAAATCA CCTTCCACAC CACAAAAACC CTCCCCATGC AAATCGACGG AGAGCCTTGG 
ATGCAGACAC CCTGTACAAT CAAGATCACC CACAAGAACC AGATGCCCAT GCTTGTGGGC 
CCACCCCCTC GCTCCTCCAA TTTCTTTGGC TTCTTATGCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DGKA ( XM_019796140.1 ) cDNA ORF clone, Ailuropoda melanoleuca(giant panda) -> XP_019651699.1 Ailuropoda melanoleuca diacylglycerol kinase alpha (DGKA), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OAc03610
Clone ID Related Accession (Same CDS sequence)	XM_002916054.3 , XM_019796140.1
Accession Version	XM_019796140.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2202bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1482768000000
Organism	Ailuropoda melanoleuca(giant panda)
Product	diacylglycerol kinase alpha isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003217407.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Ailuropoda melanoleuca Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCAAGG AGAGGGGCCT AATAAGCCCC AATGATTTTG CCCAGCTGCA AAAGTACATG 
GAATACTCCA CCAACAAGGT CAGCGATGTC CTAAAGCTCT TTGAGGATGG TGAAATGGCT 
GAATATCTCC AAGGAGATGC CATTGGGTAC GAGGGATTCC AGCAATTTTT GAAAATCTAT 
CTGGAAGCAG ATAACGTTCC CAATCACCTA AGCCTGGCAC TGTTTCAGTC CTTCAAGATG 
GGTCACTTTG AAGACACTGT GAAAAATGAT GTGGTGTATC TCAGTGATGT CTCCTGCTAC 
TTTTCCCTTC TGGAGGGTGG CCGGCCAGAA GACAAGCTAG AATTCACCTT CAAGCTGTAC 
GACACGGACA GAAATGGGAT CCTGGACAGC TCAGAAGTGG ACAGAATCAT CATACAGATG 
ATGCGAGTGG CTGAATACCT GGACTGGGAT GTGTCTGAGC TGAGGCCGAT TCTCCAGGAG 
ATGATGAAAG AGATTGACTA TGATGGCAGT GGCTCTGTCT CCCTCGCTGA GTGGCTCCGG 
GCTGGGGCGA CCACCGTGCC GCTGCTAGTG CTGCTGGGTC TGGAGATGAC TCTGAAGGAC 
AATGGGCAGC ACCTGTGGAG ACCCAAGAGG TTCCCCCGAC CGGTCTACTG CAATCTGTGC 
GAGTCGAGCA TTGGTCTTGG CAAACAGGGG CTGAGCTGTA ACCTCTGTAA GTACGTCGTT 
CACGACCAGT GTGCCATGAA GGCCTTGCCT TGTGAGGTCA GCACGTACGC CAAGTCTCGG 
AAGGACATCG GTATCCAATC GCATGTGTGG GTGAGAGGAG GCTGTGAGTC CGGCCGCTGC 
GACCGCTGTC AGAAGAAGAT CCGCATTTAC CACAGTCTCG TTGGACTGCA TTGTGTGTGG 
TGCCACCTAG AGATTCATGA TGACTGCCTA CAAGCCATGG GTCATGAATG TGACTGTGGG 
CTGCTCCGGG ATCATATCCT GCCTCCATCT TCCATCTATC CCAGTGTCCT GGCCTCTGGA 
CAGGAGCGTA AAATTAGCAA AACAAGCCAG AAGACCGTGG ATGACTTAAA TTTGAGCACC 
TTTGAGGCTC TGCGGATTGA CCCTGTTTAT AACACCCACC CACTTCTGGT CTTTGTCAAT 
CCTAAGAGTG GCGGGAAGCA GGGGGAGAGA GTGCTTTGGA AATTCCAGTA TCTACTAAAT 
CCTCGACAGG TATTCAACCT CCTAAAGGAT GGTCCTGAGC CAGGGCTCAG ATTCTTCAGG 
GATGTTCCCG GTTGCCGGAT TTTGGTGTGT GGTGGAGATG GCACAGTAGG CTGGATTCTA 
GAGACCATAG ACAAAGCCAA CTTGCCTGTT GTGCCTCCTG TTGCTGTGTT GCCCCTGGGC 
ACTGGAAATG ATCTGGCTCG TTGCCTAAGA TGGGGAGGAG GATATGAGGG ACAAAATTTG 
GGGAAGATCC TCAAGGATTT AGAGATGAGT AAGGTGGTAC ATATGGATCG ATGGTCCGTG 
GAGGTGATAC CTCAACAAAC TGAAGAAAAG AGTGACCCAG TCCCCTTTCA AATCATCAAT 
AACTACTTCT CCATCGGTGT GGATGCCTCT ATTGCTCACC GATTCCACAT CATGCGAGAG 
AAATATCCTG AGAAATTCAA CAGCAGAATG AAGAACAAGC TATGGTACTT CGAATTCGCC 
ACTTCCGAAT CCATCTTCTC AACGTGCAAA AAGCTGGAGG AGTCTTTGAC AGTCGAGATC 
TGTGGGAAAC CACTGGATCT GAGCAACCTG TCCCTAGAAG GCATCGCAGT GCTGAACATC 
CCTAGCATGC ATGGTGGTTC CAACCTCTGG GGTGATACCA AGAGACCTCA TGGAGATACC 
CATGGGATCA ACCAGGCCTT AGGCGCTGCA GCTAAAGTCA TCACTGACCC GGATATCCTG 
AAAACCTGTG TACCAGACCT AAGTGACAAG CGGCTGGAAG TAGTCGGGCT GGAGGGTGCA 
ATTGAGATGG GCCAGATTTA TACCAAGCTC AAGAATGCTG GACATCGGCT GGCCAAGTGC 
TCTGAAATCA CCTTCCACAC CACAAAAACC CTCCCCATGC AAATCGACGG AGAGCCTTGG 
ATGCAGACAC CCTGTACAAT CAAGATCACC CACAAGAACC AGATGCCCAT GCTTGTGGGC 
CCACCCCCTC GCTCCTCCAA TTTCTTTGGC TTCTTATGCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_019651699.1
CDS	294..2495
Translation	MAKERGLISPNDFAQLQKYMEYSTNKVSDVLKLFEDGEMAEYLQGDAIGYEGFQQFLKIYLEADNVPNHLSLALFQSFKMGHFEDTVKNDVVYLSDVSCYFSLLEGGRPEDKLEFTFKLYDTDRNGILDSSEVDRIIIQMMRVAEYLDWDVSELRPILQEMMKEIDYDGSGSVSLAEWLRAGATTVPLLVLLGLEMTLKDNGQHLWRPKRFPRPVYCNLCESSIGLGKQGLSCNLCKYVVHDQCAMKALPCEVSTYAKSRKDIGIQSHVWVRGGCESGRCDRCQKKIRIYHSLVGLHCVWCHLEIHDDCLQAMGHECDCGLLRDHILPPSSIYPSVLASGQERKISKTSQKTVDDLNLSTFEALRIDPVYNTHPLLVFVNPKSGGKQGERVLWKFQYLLNPRQVFNLLKDGPEPGLRFFRDVPGCRILVCGGDGTVGWILETIDKANLPVVPPVAVLPLGTGNDLARCLRWGGGYEGQNLGKILKDLEMSKVVHMDRWSVEVIPQQTEEKSDPVPFQIINNYFSIGVDASIAHRFHIMREKYPEKFNSRMKNKLWYFEFATSESIFSTCKKLEESLTVEICGKPLDLSNLSLEGIAVLNIPSMHGGSNLWGDTKRPHGDTHGINQALGAAAKVITDPDILKTCVPDLSDKRLEVVGLEGAIEMGQIYTKLKNAGHRLAKCSEITFHTTKTLPMQIDGEPWMQTPCTIKITHKNQMPMLVGPPPRSSNFFGFLC

Target ORF information:

RefSeq Version	XM_019796140.1
Organism	Ailuropoda melanoleuca(giant panda)
Definition	Ailuropoda melanoleuca diacylglycerol kinase alpha (DGKA), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_019796140.1

ORF Insert Sequence:

ATGGCCAAGG AGAGGGGCCT AATAAGCCCC AATGATTTTG CCCAGCTGCA AAAGTACATG 
GAATACTCCA CCAACAAGGT CAGCGATGTC CTAAAGCTCT TTGAGGATGG TGAAATGGCT 
GAATATCTCC AAGGAGATGC CATTGGGTAC GAGGGATTCC AGCAATTTTT GAAAATCTAT 
CTGGAAGCAG ATAACGTTCC CAATCACCTA AGCCTGGCAC TGTTTCAGTC CTTCAAGATG 
GGTCACTTTG AAGACACTGT GAAAAATGAT GTGGTGTATC TCAGTGATGT CTCCTGCTAC 
TTTTCCCTTC TGGAGGGTGG CCGGCCAGAA GACAAGCTAG AATTCACCTT CAAGCTGTAC 
GACACGGACA GAAATGGGAT CCTGGACAGC TCAGAAGTGG ACAGAATCAT CATACAGATG 
ATGCGAGTGG CTGAATACCT GGACTGGGAT GTGTCTGAGC TGAGGCCGAT TCTCCAGGAG 
ATGATGAAAG AGATTGACTA TGATGGCAGT GGCTCTGTCT CCCTCGCTGA GTGGCTCCGG 
GCTGGGGCGA CCACCGTGCC GCTGCTAGTG CTGCTGGGTC TGGAGATGAC TCTGAAGGAC 
AATGGGCAGC ACCTGTGGAG ACCCAAGAGG TTCCCCCGAC CGGTCTACTG CAATCTGTGC 
GAGTCGAGCA TTGGTCTTGG CAAACAGGGG CTGAGCTGTA ACCTCTGTAA GTACGTCGTT 
CACGACCAGT GTGCCATGAA GGCCTTGCCT TGTGAGGTCA GCACGTACGC CAAGTCTCGG 
AAGGACATCG GTATCCAATC GCATGTGTGG GTGAGAGGAG GCTGTGAGTC CGGCCGCTGC 
GACCGCTGTC AGAAGAAGAT CCGCATTTAC CACAGTCTCG TTGGACTGCA TTGTGTGTGG 
TGCCACCTAG AGATTCATGA TGACTGCCTA CAAGCCATGG GTCATGAATG TGACTGTGGG 
CTGCTCCGGG ATCATATCCT GCCTCCATCT TCCATCTATC CCAGTGTCCT GGCCTCTGGA 
CAGGAGCGTA AAATTAGCAA AACAAGCCAG AAGACCGTGG ATGACTTAAA TTTGAGCACC 
TTTGAGGCTC TGCGGATTGA CCCTGTTTAT AACACCCACC CACTTCTGGT CTTTGTCAAT 
CCTAAGAGTG GCGGGAAGCA GGGGGAGAGA GTGCTTTGGA AATTCCAGTA TCTACTAAAT 
CCTCGACAGG TATTCAACCT CCTAAAGGAT GGTCCTGAGC CAGGGCTCAG ATTCTTCAGG 
GATGTTCCCG GTTGCCGGAT TTTGGTGTGT GGTGGAGATG GCACAGTAGG CTGGATTCTA 
GAGACCATAG ACAAAGCCAA CTTGCCTGTT GTGCCTCCTG TTGCTGTGTT GCCCCTGGGC 
ACTGGAAATG ATCTGGCTCG TTGCCTAAGA TGGGGAGGAG GATATGAGGG ACAAAATTTG 
GGGAAGATCC TCAAGGATTT AGAGATGAGT AAGGTGGTAC ATATGGATCG ATGGTCCGTG 
GAGGTGATAC CTCAACAAAC TGAAGAAAAG AGTGACCCAG TCCCCTTTCA AATCATCAAT 
AACTACTTCT CCATCGGTGT GGATGCCTCT ATTGCTCACC GATTCCACAT CATGCGAGAG 
AAATATCCTG AGAAATTCAA CAGCAGAATG AAGAACAAGC TATGGTACTT CGAATTCGCC 
ACTTCCGAAT CCATCTTCTC AACGTGCAAA AAGCTGGAGG AGTCTTTGAC AGTCGAGATC 
TGTGGGAAAC CACTGGATCT GAGCAACCTG TCCCTAGAAG GCATCGCAGT GCTGAACATC 
CCTAGCATGC ATGGTGGTTC CAACCTCTGG GGTGATACCA AGAGACCTCA TGGAGATACC 
CATGGGATCA ACCAGGCCTT AGGCGCTGCA GCTAAAGTCA TCACTGACCC GGATATCCTG 
AAAACCTGTG TACCAGACCT AAGTGACAAG CGGCTGGAAG TAGTCGGGCT GGAGGGTGCA 
ATTGAGATGG GCCAGATTTA TACCAAGCTC AAGAATGCTG GACATCGGCT GGCCAAGTGC 
TCTGAAATCA CCTTCCACAC CACAAAAACC CTCCCCATGC AAATCGACGG AGAGCCTTGG 
ATGCAGACAC CCTGTACAAT CAAGATCACC CACAAGAACC AGATGCCCAT GCTTGTGGGC 
CCACCCCCTC GCTCCTCCAA TTTCTTTGGC TTCTTATGCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DGKA ( XM_019796141.1 ) cDNA ORF clone, Ailuropoda melanoleuca(giant panda) -> XP_019651700.1 Ailuropoda melanoleuca diacylglycerol kinase alpha (DGKA), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OAc90502
Clone ID Related Accession (Same CDS sequence)	XM_019796141.1
Accession Version	XM_019796141.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1965bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1482768000000
Organism	Ailuropoda melanoleuca(giant panda)
Product	diacylglycerol kinase alpha isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003217407.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Ailuropoda melanoleuca Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGTCACT TTGAAGACAC TGTGAAAAAT GATGTGGTGT ATCTCAGTGA TGTCTCCTGC 
TACTTTTCCC TTCTGGAGGG TGGCCGGCCA GAAGACAAGC TAGAATTCAC CTTCAAGCTG 
TACGACACGG ACAGAAATGG GATCCTGGAC AGCTCAGAAG TGGACAGAAT CATCATACAG 
ATGATGCGAG TGGCTGAATA CCTGGACTGG GATGTGTCTG AGCTGAGGCC GATTCTCCAG 
GAGATGATGA AAGAGATTGA CTATGATGGC AGTGGCTCTG TCTCCCTCGC TGAGTGGCTC 
CGGGCTGGGG CGACCACCGT GCCGCTGCTA GTGCTGCTGG GTCTGGAGAT GACTCTGAAG 
GACAATGGGC AGCACCTGTG GAGACCCAAG AGGTTCCCCC GACCGGTCTA CTGCAATCTG 
TGCGAGTCGA GCATTGGTCT TGGCAAACAG GGGCTGAGCT GTAACCTCTG TAAGTACGTC 
GTTCACGACC AGTGTGCCAT GAAGGCCTTG CCTTGTGAGG TCAGCACGTA CGCCAAGTCT 
CGGAAGGACA TCGGTATCCA ATCGCATGTG TGGGTGAGAG GAGGCTGTGA GTCCGGCCGC 
TGCGACCGCT GTCAGAAGAA GATCCGCATT TACCACAGTC TCGTTGGACT GCATTGTGTG 
TGGTGCCACC TAGAGATTCA TGATGACTGC CTACAAGCCA TGGGTCATGA ATGTGACTGT 
GGGCTGCTCC GGGATCATAT CCTGCCTCCA TCTTCCATCT ATCCCAGTGT CCTGGCCTCT 
GGACAGGAGC GTAAAATTAG CAAAACAAGC CAGAAGACCG TGGATGACTT AAATTTGAGC 
ACCTTTGAGG CTCTGCGGAT TGACCCTGTT TATAACACCC ACCCACTTCT GGTCTTTGTC 
AATCCTAAGA GTGGCGGGAA GCAGGGGGAG AGAGTGCTTT GGAAATTCCA GTATCTACTA 
AATCCTCGAC AGGTATTCAA CCTCCTAAAG GATGGTCCTG AGCCAGGGCT CAGATTCTTC 
AGGGATGTTC CCGGTTGCCG GATTTTGGTG TGTGGTGGAG ATGGCACAGT AGGCTGGATT 
CTAGAGACCA TAGACAAAGC CAACTTGCCT GTTGTGCCTC CTGTTGCTGT GTTGCCCCTG 
GGCACTGGAA ATGATCTGGC TCGTTGCCTA AGATGGGGAG GAGGATATGA GGGACAAAAT 
TTGGGGAAGA TCCTCAAGGA TTTAGAGATG AGTAAGGTGG TACATATGGA TCGATGGTCC 
GTGGAGGTGA TACCTCAACA AACTGAAGAA AAGAGTGACC CAGTCCCCTT TCAAATCATC 
AATAACTACT TCTCCATCGG TGTGGATGCC TCTATTGCTC ACCGATTCCA CATCATGCGA 
GAGAAATATC CTGAGAAATT CAACAGCAGA ATGAAGAACA AGCTATGGTA CTTCGAATTC 
GCCACTTCCG AATCCATCTT CTCAACGTGC AAAAAGCTGG AGGAGTCTTT GACAGTCGAG 
ATCTGTGGGA AACCACTGGA TCTGAGCAAC CTGTCCCTAG AAGGCATCGC AGTGCTGAAC 
ATCCCTAGCA TGCATGGTGG TTCCAACCTC TGGGGTGATA CCAAGAGACC TCATGGAGAT 
ACCCATGGGA TCAACCAGGC CTTAGGCGCT GCAGCTAAAG TCATCACTGA CCCGGATATC 
CTGAAAACCT GTGTACCAGA CCTAAGTGAC AAGCGGCTGG AAGTAGTCGG GCTGGAGGGT 
GCAATTGAGA TGGGCCAGAT TTATACCAAG CTCAAGAATG CTGGACATCG GCTGGCCAAG 
TGCTCTGAAA TCACCTTCCA CACCACAAAA ACCCTCCCCA TGCAAATCGA CGGAGAGCCT 
TGGATGCAGA CACCCTGTAC AATCAAGATC ACCCACAAGA ACCAGATGCC CATGCTTGTG 
GGCCCACCCC CTCGCTCCTC CAATTTCTTT GGCTTCTTAT GCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_019651700.1
CDS	358..2322
Translation	MGHFEDTVKNDVVYLSDVSCYFSLLEGGRPEDKLEFTFKLYDTDRNGILDSSEVDRIIIQMMRVAEYLDWDVSELRPILQEMMKEIDYDGSGSVSLAEWLRAGATTVPLLVLLGLEMTLKDNGQHLWRPKRFPRPVYCNLCESSIGLGKQGLSCNLCKYVVHDQCAMKALPCEVSTYAKSRKDIGIQSHVWVRGGCESGRCDRCQKKIRIYHSLVGLHCVWCHLEIHDDCLQAMGHECDCGLLRDHILPPSSIYPSVLASGQERKISKTSQKTVDDLNLSTFEALRIDPVYNTHPLLVFVNPKSGGKQGERVLWKFQYLLNPRQVFNLLKDGPEPGLRFFRDVPGCRILVCGGDGTVGWILETIDKANLPVVPPVAVLPLGTGNDLARCLRWGGGYEGQNLGKILKDLEMSKVVHMDRWSVEVIPQQTEEKSDPVPFQIINNYFSIGVDASIAHRFHIMREKYPEKFNSRMKNKLWYFEFATSESIFSTCKKLEESLTVEICGKPLDLSNLSLEGIAVLNIPSMHGGSNLWGDTKRPHGDTHGINQALGAAAKVITDPDILKTCVPDLSDKRLEVVGLEGAIEMGQIYTKLKNAGHRLAKCSEITFHTTKTLPMQIDGEPWMQTPCTIKITHKNQMPMLVGPPPRSSNFFGFLC

Target ORF information:

RefSeq Version	XM_019796141.1
Organism	Ailuropoda melanoleuca(giant panda)
Definition	Ailuropoda melanoleuca diacylglycerol kinase alpha (DGKA), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_019796141.1

ORF Insert Sequence:

ATGGGTCACT TTGAAGACAC TGTGAAAAAT GATGTGGTGT ATCTCAGTGA TGTCTCCTGC 
TACTTTTCCC TTCTGGAGGG TGGCCGGCCA GAAGACAAGC TAGAATTCAC CTTCAAGCTG 
TACGACACGG ACAGAAATGG GATCCTGGAC AGCTCAGAAG TGGACAGAAT CATCATACAG 
ATGATGCGAG TGGCTGAATA CCTGGACTGG GATGTGTCTG AGCTGAGGCC GATTCTCCAG 
GAGATGATGA AAGAGATTGA CTATGATGGC AGTGGCTCTG TCTCCCTCGC TGAGTGGCTC 
CGGGCTGGGG CGACCACCGT GCCGCTGCTA GTGCTGCTGG GTCTGGAGAT GACTCTGAAG 
GACAATGGGC AGCACCTGTG GAGACCCAAG AGGTTCCCCC GACCGGTCTA CTGCAATCTG 
TGCGAGTCGA GCATTGGTCT TGGCAAACAG GGGCTGAGCT GTAACCTCTG TAAGTACGTC 
GTTCACGACC AGTGTGCCAT GAAGGCCTTG CCTTGTGAGG TCAGCACGTA CGCCAAGTCT 
CGGAAGGACA TCGGTATCCA ATCGCATGTG TGGGTGAGAG GAGGCTGTGA GTCCGGCCGC 
TGCGACCGCT GTCAGAAGAA GATCCGCATT TACCACAGTC TCGTTGGACT GCATTGTGTG 
TGGTGCCACC TAGAGATTCA TGATGACTGC CTACAAGCCA TGGGTCATGA ATGTGACTGT 
GGGCTGCTCC GGGATCATAT CCTGCCTCCA TCTTCCATCT ATCCCAGTGT CCTGGCCTCT 
GGACAGGAGC GTAAAATTAG CAAAACAAGC CAGAAGACCG TGGATGACTT AAATTTGAGC 
ACCTTTGAGG CTCTGCGGAT TGACCCTGTT TATAACACCC ACCCACTTCT GGTCTTTGTC 
AATCCTAAGA GTGGCGGGAA GCAGGGGGAG AGAGTGCTTT GGAAATTCCA GTATCTACTA 
AATCCTCGAC AGGTATTCAA CCTCCTAAAG GATGGTCCTG AGCCAGGGCT CAGATTCTTC 
AGGGATGTTC CCGGTTGCCG GATTTTGGTG TGTGGTGGAG ATGGCACAGT AGGCTGGATT 
CTAGAGACCA TAGACAAAGC CAACTTGCCT GTTGTGCCTC CTGTTGCTGT GTTGCCCCTG 
GGCACTGGAA ATGATCTGGC TCGTTGCCTA AGATGGGGAG GAGGATATGA GGGACAAAAT 
TTGGGGAAGA TCCTCAAGGA TTTAGAGATG AGTAAGGTGG TACATATGGA TCGATGGTCC 
GTGGAGGTGA TACCTCAACA AACTGAAGAA AAGAGTGACC CAGTCCCCTT TCAAATCATC 
AATAACTACT TCTCCATCGG TGTGGATGCC TCTATTGCTC ACCGATTCCA CATCATGCGA 
GAGAAATATC CTGAGAAATT CAACAGCAGA ATGAAGAACA AGCTATGGTA CTTCGAATTC 
GCCACTTCCG AATCCATCTT CTCAACGTGC AAAAAGCTGG AGGAGTCTTT GACAGTCGAG 
ATCTGTGGGA AACCACTGGA TCTGAGCAAC CTGTCCCTAG AAGGCATCGC AGTGCTGAAC 
ATCCCTAGCA TGCATGGTGG TTCCAACCTC TGGGGTGATA CCAAGAGACC TCATGGAGAT 
ACCCATGGGA TCAACCAGGC CTTAGGCGCT GCAGCTAAAG TCATCACTGA CCCGGATATC 
CTGAAAACCT GTGTACCAGA CCTAAGTGAC AAGCGGCTGG AAGTAGTCGG GCTGGAGGGT 
GCAATTGAGA TGGGCCAGAT TTATACCAAG CTCAAGAATG CTGGACATCG GCTGGCCAAG 
TGCTCTGAAA TCACCTTCCA CACCACAAAA ACCCTCCCCA TGCAAATCGA CGGAGAGCCT 
TGGATGCAGA CACCCTGTAC AATCAAGATC ACCCACAAGA ACCAGATGCC CATGCTTGTG 
GGCCCACCCC CTCGCTCCTC CAATTTCTTT GGCTTCTTAT GCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

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DGKA cDNA ORF clone, Ailuropoda melanoleuca(giant panda)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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