SPG11 cDNA ORF clone, Canis lupus familiaris(dog)

Gene
Clones
gRNAs

Summary

Gene Symbol	SPG11
Entrez Gene ID	100856719
Full Name	SPG11, spatacsin vesicle trafficking associated
General protein information	Preferred Names SPG11, spatacsin vesicle trafficking associated Names spatacsin spastic paraplegia 11 (autosomal recessive)
Gene Type	protein-coding
Organism	Canis lupus familiaris(dog)

mRNA and Protein(s)

mRNA	Protein	Name
XM_003640025.3	XP_003640073.2	spatacsin isoform X1
XM_003640025.4	XP_003640073.2	spatacsin isoform X1
XM_014109490.1	XP_013964965.1	spatacsin isoform X2
XM_005638363.2	XP_005638420.1	spatacsin isoform X4
XM_005638363.3	XP_005638420.1	spatacsin isoform X4
XM_014109491.1	XP_013964966.1	spatacsin isoform X3
XM_014109491.2	XP_013964966.1	spatacsin isoform X3
XM_014109490.2	XP_013964965.1	spatacsin isoform X2

Pathways from BioSystems

Homologies

Rattus norvegicus (Norway rat)	Spg11	NP_001178500.1
Homo sapiens (human)	SPG11	NP_079413.3
Bos taurus (cattle)	SPG11	XP_002691138.2
Mus musculus (house mouse)	Spg11	NP_663506.2
Canis lupus familiaris (dog)	SPG11	XP_003640073.2
Macaca mulatta (Rhesus monkey)	LOC712221	XP_002808495.1
Gallus gallus (chicken)	SPG11	XP_003641879.2
Danio rerio (zebrafish)	spg11	NP_001181923.1

Gene Ontology

The following SPG11 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the SPG11 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OCe28791	XM_003640025.4 Latest version!	Canis lupus familiaris SPG11, spatacsin vesicle trafficking associated (SPG11), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1301.30 $1859.00
OCe28793	XM_005638363.3 Latest version!	Canis lupus familiaris SPG11, spatacsin vesicle trafficking associated (SPG11), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OCe44278	XM_014109490.2 Latest version!	Canis lupus familiaris SPG11, spatacsin vesicle trafficking associated (SPG11), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1301.30 $1859.00
OCe44279	XM_014109491.2 Latest version!	Canis lupus familiaris SPG11, spatacsin vesicle trafficking associated (SPG11), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1301.30 $1859.00
View Old Accession Versions >>
OCe28791	XM_003640025.3	Canis lupus familiaris spastic paraplegia 11 (autosomal recessive) (SPG11), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1301.30 $1859.00
OCe28793	XM_005638363.2	Canis lupus familiaris spastic paraplegia 11 (autosomal recessive) (SPG11), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OCe44279	XM_014109491.1	Canis lupus familiaris spastic paraplegia 11 (autosomal recessive) (SPG11), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1301.30 $1859.00
OCe44278	XM_014109490.1	Canis lupus familiaris spastic paraplegia 11 (autosomal recessive) (SPG11), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1301.30 $1859.00
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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

SPG11 ( XM_003640025.3 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_003640073.2 Canis lupus familiaris spastic paraplegia 11 (autosomal recessive) (SPG11), transcript variant X1, mRNA.

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CloneID	OCe28791
Clone ID Related Accession (Same CDS sequence)	XM_003640025.3 , XM_003640025.4
Accession Version	XM_003640025.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7326bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1442332800000
Organism	Canis lupus familiaris(Dog)
Product	spatacsin isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726113.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_003640025.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCGCAG GAGAGGCAGC TGCGAGAGCT GCTGGTTGCA GCAGCTGCGG CGCTGAAACT 
ATGGAACGGG TTCTACCTAT GCTGTTGGTG CCGGTCCCTG TGGAGGCAAC CGGGCGGTTG 
GGCTCCCGGG CGCAGTTGCA CAGAGAGCAG GAGGTTCTGG GGAGCCTAAC AGCTGCAGGC 
AGCCTGCAGG TGCTTTCCTT GGCGCCGGGC GGCCGGAGCG GCGGGGCCTG CTGCCTCAAT 
GGCCCCTTTC GGCACTTTAT ATGGGAGGAT TCTCATAACG GCAGCTCACC AACAGACAAG 
CCCAAACTTC TTGCTCTTAG TGAAAACTAT GAACTGTTTA TCTATGAATT TAATTTGAAA 
GATGGAAGAT GTGATGCAAC CATTTTGTAT AGCTGTAGTG AGGAGACATT GCAGAAGCTC 
ATTGAAGATC AAAATATCAG TGTTTCCTTT TTATCTTTGA GAATTCTGTC ATTTCACAAT 
AACACATCAT TATTGTTCAT CAATAAATGT ATTGTCCTAC ACATTATATT TCCTGAAAGA 
GATGCTGAGA TCAGAGTACT CAACTGTTTC ACGTTACCCT TGCCTGCACA GGCAGTGGAC 
AGGATCATTG ATGTGCAGCT CTGCAGAGGA ATTCTTTTTG TTTTGAGTAG TTTAGGCTGG 
ATCTACATTT TTGACATTGT GGATGGAAGA CATGTAGCTC ACGTGGATTT AGCACTTCAC 
CAAGAAGATA TGTGTAATGA ACAGCAACAG GAACTAGCCA AGATTTCTTC CTTTACTTCA 
CTGAAAGTGT CTCAAGACCT AGATGTTGTA GTGATTGTCA GCTCCTTCAA CTCTGCAGTT 
GCTCTTAACC TAAACTTGTA TTTCAGGCAA CACCCAGGAC ACCTACTGTG TGAAAGAACT 
CTAGAAGACA TTCCTATTGA AGGCCCTGAG GGGATCGATG AAGATGATCC TGTTAATTCT 
GACTACAACA TGAAACTGAC CAAGTTTTCC TTTCAAGTTG ATAGGTCTTG GAAAGCCCAG 
CTATCATCAC TGAATGAAAT AATAAAGAGC TCCAAATGGG AGGTTCCCTG TTGTGCTCCA 
TGGTTCCAGA ATATTTTGCA TTTGGAGTCA CCGGAATCTG GTTACCACAG TACTAGTACA 
CCAGGCTGGG CCTTCATCCC ACAGGATGAA ATTCATAGCC ACTACAAGTT TCCACAGAAA 
GATCATGCCA ACACCGGTGA CCCAGGAAGA TCATGGAAAA TAATGAACAT TAGTGAACTA 
AAGGAACCTG TTGAGCTTAC ATGTATGTCT GTGACAGGAT TCAGTGTACT GTTTACTTGG 
GCAGTGGAAA AGATGGGCTG CACCATTGTC CTTTGGGATT TGGAGACCCA GGGTATCCAG 
TGTTTTTCCC TTGGCAAAAG GTGTATTCCT ATAGATATGA GTGGAGACCA GCAGCTGTGC 
CTTGTTTTGA CAGAAAATGG ACTCTCCCTG ATTTTGTTTG GATTGACTCA GGAGGAGTTT 
TTAAATAGAC TAATGATCCA TGGAAGTGCC AGCACTGTGG ACTCTCTTTG CCATCTCAAT 
GGTTGGGGGA GGTGCTCAAT CCCCATACAT GCGCTGGAGG CTGGGATAGA AAATCGTCAG 
CTGGACACCG TAGATTTCTT TTTGAAGAGC AAAGAAAATC TTTTGAATCC ATCCTCAAAA 
TCTTCCGTAC CGGATCAGTT TGATGACTTT CCATCTCATT TATATTTAAG AAATGTGGAA 
AAGCTGACAC CAGCATTGGA TTTACTTTGC TCAGCAATTA GAGAAAGTGA TTCTGAAACC 
CAAAGCAAAC ACTTTTCAGA ACAGTTGCTT AACCTTACAC TGTCTTTTCT TAACAAACAA 
ATAAAGGAGC TTTTTGTTCA CAATGAAGAA TTAGATGAAC ATCTACAGAA AGGAGTGAAC 
ATTTTGACCA GCTACATTAA TGAACTTCGA ACCTTCATGA TAAAATTTCC TTGGAAGATA 
GTAGACCCAG TAGATGAATA TGATGTAAGT GAAAATGTCC TCAAAGTAAG AGAGAGTGAT 
ACATGGGAGA AACTCAGCAT TGAGGAAGTT ATTGCCAATG CTATTTTAAA CAACAAAATA 
CCAGAGGCAC AGACTTTCTT CAGGATTAAC AATCATTCTG CTCAAAGACT TGAGGAGCTG 
ATTAGAATAG GCCTAAATTT GGTCTTTGAC TGTTTGAAAA AGAACAATAT AAAGGAAGCA 
TCTGAACTTT TGAAGAATAT GGGCTTTGAT GTAAAAGACC AGTTGCTCAA GATATGTTTC 
TATACAACTG ATAAAAATAT ACGGGACTTT TTGGTGGAAA TTTTAAAAGA AAAAAATTAT 
TTTTCTGAAA ATGAAAAAAG AACTATAGAC TTCGTGCATC AAGTTGAGAA GTTTTATTCA 
GGACATTTTC AAGAAAATGT GCAGATCCAG TCCTTTACCA GGTATTGGAT AAAGGAACAG 
GATTTTTTCA AGCACAAATC TGTTTTGGAC TCACTCCTGA AATATGATCC TAAAGATAAA 
TTTAACAAAC AGGACCATAG AATTGTGTTA AATTGGGCTC AGTGGTGGAA TCAACTAACA 
CAAGAATCCA TCCTTCTCCC CAGGATAAGT CCAGAAGAAT ACAAATCATG TTCTCCTGAA 
GCCCTTTGGA GCTATCTCAC AGCCCATCAT GATTGGTCAA GCATTAGCTT GTGGATTGAA 
GAATTTCAGG CCCAGAACAG TTGTGTTTTA CTGCAGCAGA ACAAATGGCC CCTTCTGACT 
GTTGATGTTA TAGACCAGAA TACTTGCTGC AACAACTACA TGAGGAATGA AATTTTAGAT 
CAGCTGGCCA GGAATGGGAT TTTTCTTCAA TCTGAGCTAG AAGACTTTGA ACTCTTCCTG 
CTAAGACTGA ATCGCATTGG GGGTGTGATG CAAGATGCTC TCCCCATTCA AAACTACAGA 
TCCAAAGAAG GTTGGGATTT CCATTCTCAC TTCATTCTCT ATTGTTTGGA ACATGGTCTG 
CAGTATCCTC TCTATGTCTA TCTTGACTTT TACAAACTTA GTCCTGCAAA TTGTCCCTTT 
TTGGAAAAAA AAGAATTACA TGAAACTCAC CCTTGGTTTG AATTTTTGGT TCAGTGTCGA 
CAGGTTTCCA GTAATTTAAC AGATCCCAAA CTGATCTTCC AGGCTAGCCT TGCAAACGCT 
CAGATACTGA TTCCCAGCAA CCAGGCCAGT GTAAGCAGTA TGCTATTGGA AGGACATACT 
CTCCTGGCCC TTGCTACCAC AATGTATGCC CCTGGCGGTG TCAGCCAGGT TGTTCAGGAT 
GATGAAAATG AGACCTGTTT GAAGAAAGTA GATCCCCAGT TACTGAAGAT GGCATTAACT 
CCTTATCCCA AGCTAAAAAC TGCTCTCTTC CCACAGTACA CTACCTCTAA TGTTCTGCCG 
CCTGATATTA CGCTTTACCA CCTTATTCAG TCACTATTGC CCTTTGACTC TAGCAGATTG 
TTTGGCTGGC AGTCTGCCAA CACACTGGCT ATTGGAGATG CAGTGAGTCA TCTCCCACAT 
TTTTCTAGCC CTGATCTGGT TAATAAATAT GCTATCATGG AACGTCTGAA TTTTGCATAT 
TATTTACAAC ATGGACGGCC GTCATTTGCA TTTGGTACTT TTCTGGTCCA GGAATTAACC 
AAGAGCAAGA CTCCCAAGCA GCTGATCCAG CAAGTAGGCA ATGAAGCTTA TGTTTTAGGG 
CTCTCCTCCT TCTACATTCC ATCAGTAGGA GCTGCCTGTG TTTGTTTCTT AGAACTGCTT 
GGCCTCGATA GCCTCAAGCT CAGAGTTGAC ATGAAAGTGG CCAATATAAT TTTGAACTAC 
AAATGTAGAA ATGAAGATGT TCAGTACAGC TGTATCAGAG AGGCTCTAGT TGAAAAACTA 
TCTAAACTGG CTGTGGGTGA AAAGGCCACC GTAGAGGAAT TGCTTATTCT CTTAGAAGAA 
GGTATATGGA ACAACATTCA GCAGCAAGAG ATAAAGAGGT TATCTAGTGA ATCTAGCAGC 
CAGTGGGCAT TAGCAGTACA GTTCTGCAGG CTTCACAATG TGAAGCTGAG CACGTCTTAC 
CTTAGAGAAT GTGCCAAAGC AAATGACTGG TTACAGTTCA TTATTCACAG CCAACTCTAT 
AACTATCACC CAGCGGAGGT GAAATCTCTT CTTCAGTATT TCAGTCCTGT CCTTCAAGAC 
CACTTAAGAC TAGCTTTTGA GAACCTGACC TCAGTGTCTA AATCCAAAAT GGATAGTGGT 
GAAGTCTGCA GCAGGTCTCC CCAGGAACTC CAGAGAAACA AAGAAGAAAT GGCTGATTTC 
TTTCAAGTTC TACTCCAGTG CTCAGAGGAA CCAAACCCCT GGTGCTGGCT CCTAGCTGAA 
GCAATGAAAC AACAGGCTCC TATCCTTAGT GTCCTGGCCT CATGTCTTCA GGATGCCAGT 
ACTATTCCTT GTCTCTGTGT TTGGATCATC ACATCTGTGG AGGACAGTGT TGCAGCTGAA 
GCAACAGAAC ACATTCAGGG CTCAGTGGAA GGCCACATAT GGGACCTTCA AGACCTCTCA 
GTCATCTGGA GAACATTATT AACAAGGCAG AAGAACAAAA CTCTCATCAG AGGTTTCCAA 
CTTTTCTTTA AGGATTCCCC ACTACTGTTG ATGATGGAGA TGTATGAACT GTGTATGTTC 
TTCAAGAATT ATAAAGAAGC TGAAGCTAAA CTTCTGGAGT TCCAAAAGAG CTTTGAAACT 
GTTGAAACAG CAGCCACAAG GGTCCACACT ACCATCCCTG CCCCATGGCT GAGGGAGCAG 
GTGTGTTTTC TTCTGAAGCT TATGCTGCAG CAGTGTGGGA CCCAGTATGA ACTGGGGAAG 
CTTTTACAGC TATTTGTTGG AATAGAAAAT TTCTTCTCTG ATGGTCCAGA TGTGAAGAAG 
CTCTGTGTCC TTAGTGAAAT TTTGAAGGAC ACATCCATAG CCATTAATCA TGTAATTATT 
AGCAGCCACA GCCTTGAGAA TTTTCAGCAT GAATGTACAT CTATTTTGGA AAAACTGGCG 
ATGGAGGGAC AGTTTGCTTT GGCCAGGAGA GTAGCAGAAT TAGCTGAATT ACCTGTGGAC 
AACTTGGTTA TTGAAGAGAT GACACAGGAA ATGCAAACCT TAAAACACAT TGACCAGTGG 
TCCCTGAAAC AAGCAAGAAT TGACTTCTGG AAAAAATGTC ATGAGAATTT TAAGAAAAAT 
TCCATTTCAA GCAAAGCAGC TTCTTCCTTT TTCTCAGCCC AGGCACCTGA GTGCCCAACT 
GAAGAGGGTC GGAGCAGCAG TGAGGAGCGC CATCTGCTGC TCACATTGGC AGCACACTGG 
CTTGCTCAGG AGGATCTGGT GCCGGTGGCC GAACTGGAGG AGCTAGAGAA GCAGATCTGG 
CTCTGCCGCA TTACCCAATA CACCCTCAGA GAAAAACAGG AGCAAGTAGA GCTCGGGTTT 
TCTCAACAGA TTTCAACTAG TGGGGAACTT TCCTTTGATG GCATAGCCAG TAAGTTTTCA 
CTCTCCATGT TGGCTGTTCT GAACACATCA AAGTACTTAG AACTCAGTGG CTTTCCATCC 
AAAGAGACAT GTGATAGCGG ACTGGATTGG AAGGAGCAGG AGTCACTGAA CTTTCTGATT 
GGGCACCTAT TGGATGATGG CTTTGTGCAT GAAGCAAGTA GAGTGTGTCG GTATTTTCAT 
TTCTATAACC AAGATGTTGC CTTGGTGTTG CACTGCAGAG CACTGGCCTC AGGGGAAGCT 
AGTGTGGATG ACCTGCACCC AGAGATCCAT GCTCTCCTAC AAAGTGCTGA GCCGCTTGAG 
GAGGAGGAAG CTGGCATTCC ACTAAGAAAA GTCCAGAGCA GTTCAAGTTT GGATAGTCAG 
TCATTTGTGA TGCTGCCCCC CAGTGATGAA GTGGTGACTA ATCTGGAAAC TCTGACAAGT 
AAATGCCTCC ATGGGAAGAA CTACTGTCGG CAGGTCCTGT GTCTGTATGA ACTTGCCAAG 
GAGTTGGGCT GTTCCTACAC AGACGTGGCC TCTCAGGACG GGGAGGCCAT GCTCCGGGCA 
ATCTTGGCTT CCCAGCAGCC CGACCGATGC AAGCGAGCCC AGGCTTTCAT TAGCACCCAG 
GGCCTCGAGG CAGACACGGT GGCTGAACTT GTGGCTGAAG AGGTGACCCG GGAGCTGCTG 
ACCCCACCGG AGGGAACAGG ACATCAGCAG ATGTTTGCCC CAGCAGAGGA AAGCCGGACA 
TTTCTTCAGC TGACCACTCT GTGTCAAGAC CGCACACTGG TAGGCATGAA GTTGTTGGAC 
AAGATTTCGT CCGTTCCCCA TGGAGAATTA TCTTGCACCA CAGAGCTGCT GATCCTGGCC 
CATCACTGCT TCACACTGAC ATGCCACATG GAGGGCATCA TCCGAGTCCT GCAAGCTGCC 
CGGCTGCTCA CGGACAAACA CTTGGCCCCC AATGAAGAGT ACGGGCTGAT GGTACGCCTC 
CTCACCGGCA TCGGAAGGTA CAACGAGATG ACGTACATAT TTGACTTGCT GCATAATAAG 
CACTATTTTG AAGTGCTGAT GAGGAAGAAG TTGGATCCGA GTGGCTCCCT GAAGACCGCC 
CTACTGGACT ACATCAAACG CTGCCGCCCT GGGGACAGTG AGAAGCACAA CATGATCGCT 
CTGTGCTTCA GCATGTGTCG GGAGATTGGG GAGAACCATG AGGCAGCTGC CCGCATCCAG 
CTGAAACTGA TCGAGTCTCA GCCCTGGGAG GACAGCCTCA AGGACGGGCA CCACCTGAAG 
CAGCTGCTGC TGAAAGCCCT GACTCTGATG CTGGATGCAG CCGAGAGCTA TGCCAAGGAC 
TCCTGTGTGC GACAGGCCCA GCACTGTCAC CGGCTCACCA AGTTGATAAC ACTGCAGATT 
CATTTTCTGA ACACTGGCCA GAACACAATG CTCATCAACC TGGGCCGCCA CCGGCTGATG 
GACTGTATCC TCGCTCTACC TCGGTTCTAC CAGGCTTCTA TTGTGGCCGA AGCCTATGAC 
TTTGTTCCTG ATTGGGCAGA AATCCTCTAC CAACAAGTGA TTCTTAAAGG AGACTTTAAT 
TACCTGGAAG AATTTAAGCA GCAAAGGTTA TTAAGATCCA GTATATTTGA AGAGATTTCA 
AAAAAATATA AACAACATCA GCCGACAGAC ACGGCTGTGA AAAACCTGAA GAAGTTACTC 
ACATACTGTG AAGATGTCTA CTTGTACTAC AAGCTCGCAT ATGAACACAA ATTTTATGAC 
ATTGTGAATG TGCTTCTGAA GGACCCTCAG AGCGGCTGCT GTCTAAAGGA CATGCTAGCA 
GGTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_003640073.2
CDS	12..7337
Translation	MAAGEAAARAAGCSSCGAETMERVLPMLLVPVPVEATGRLGSRAQLHREQEVLGSLTAAGSLQVLSLAPGGRSGGACCLNGPFRHFIWEDSHNGSSPTDKPKLLALSENYELFIYEFNLKDGRCDATILYSCSEETLQKLIEDQNISVSFLSLRILSFHNNTSLLFINKCIVLHIIFPERDAEIRVLNCFTLPLPAQAVDRIIDVQLCRGILFVLSSLGWIYIFDIVDGRHVAHVDLALHQEDMCNEQQQELAKISSFTSLKVSQDLDVVVIVSSFNSAVALNLNLYFRQHPGHLLCERTLEDIPIEGPEGIDEDDPVNSDYNMKLTKFSFQVDRSWKAQLSSLNEIIKSSKWEVPCCAPWFQNILHLESPESGYHSTSTPGWAFIPQDEIHSHYKFPQKDHANTGDPGRSWKIMNISELKEPVELTCMSVTGFSVLFTWAVEKMGCTIVLWDLETQGIQCFSLGKRCIPIDMSGDQQLCLVLTENGLSLILFGLTQEEFLNRLMIHGSASTVDSLCHLNGWGRCSIPIHALEAGIENRQLDTVDFFLKSKENLLNPSSKSSVPDQFDDFPSHLYLRNVEKLTPALDLLCSAIRESDSETQSKHFSEQLLNLTLSFLNKQIKELFVHNEELDEHLQKGVNILTSYINELRTFMIKFPWKIVDPVDEYDVSENVLKVRESDTWEKLSIEEVIANAILNNKIPEAQTFFRINNHSAQRLEELIRIGLNLVFDCLKKNNIKEASELLKNMGFDVKDQLLKICFYTTDKNIRDFLVEILKEKNYFSENEKRTIDFVHQVEKFYSGHFQENVQIQSFTRYWIKEQDFFKHKSVLDSLLKYDPKDKFNKQDHRIVLNWAQWWNQLTQESILLPRISPEEYKSCSPEALWSYLTAHHDWSSISLWIEEFQAQNSCVLLQQNKWPLLTVDVIDQNTCCNNYMRNEILDQLARNGIFLQSELEDFELFLLRLNRIGGVMQDALPIQNYRSKEGWDFHSHFILYCLEHGLQYPLYVYLDFYKLSPANCPFLEKKELHETHPWFEFLVQCRQVSSNLTDPKLIFQASLANAQILIPSNQASVSSMLLEGHTLLALATTMYAPGGVSQVVQDDENETCLKKVDPQLLKMALTPYPKLKTALFPQYTTSNVLPPDITLYHLIQSLLPFDSSRLFGWQSANTLAIGDAVSHLPHFSSPDLVNKYAIMERLNFAYYLQHGRPSFAFGTFLVQELTKSKTPKQLIQQVGNEAYVLGLSSFYIPSVGAACVCFLELLGLDSLKLRVDMKVANIILNYKCRNEDVQYSCIREALVEKLSKLAVGEKATVEELLILLEEGIWNNIQQQEIKRLSSESSSQWALAVQFCRLHNVKLSTSYLRECAKANDWLQFIIHSQLYNYHPAEVKSLLQYFSPVLQDHLRLAFENLTSVSKSKMDSGEVCSRSPQELQRNKEEMADFFQVLLQCSEEPNPWCWLLAEAMKQQAPILSVLASCLQDASTIPCLCVWIITSVEDSVAAEATEHIQGSVEGHIWDLQDLSVIWRTLLTRQKNKTLIRGFQLFFKDSPLLLMMEMYELCMFFKNYKEAEAKLLEFQKSFETVETAATRVHTTIPAPWLREQVCFLLKLMLQQCGTQYELGKLLQLFVGIENFFSDGPDVKKLCVLSEILKDTSIAINHVIISSHSLENFQHECTSILEKLAMEGQFALARRVAELAELPVDNLVIEEMTQEMQTLKHIDQWSLKQARIDFWKKCHENFKKNSISSKAASSFFSAQAPECPTEEGRSSSEERHLLLTLAAHWLAQEDLVPVAELEELEKQIWLCRITQYTLREKQEQVELGFSQQISTSGELSFDGIASKFSLSMLAVLNTSKYLELSGFPSKETCDSGLDWKEQESLNFLIGHLLDDGFVHEASRVCRYFHFYNQDVALVLHCRALASGEASVDDLHPEIHALLQSAEPLEEEEAGIPLRKVQSSSSLDSQSFVMLPPSDEVVTNLETLTSKCLHGKNYCRQVLCLYELAKELGCSYTDVASQDGEAMLRAILASQQPDRCKRAQAFISTQGLEADTVAELVAEEVTRELLTPPEGTGHQQMFAPAEESRTFLQLTTLCQDRTLVGMKLLDKISSVPHGELSCTTELLILAHHCFTLTCHMEGIIRVLQAARLLTDKHLAPNEEYGLMVRLLTGIGRYNEMTYIFDLLHNKHYFEVLMRKKLDPSGSLKTALLDYIKRCRPGDSEKHNMIALCFSMCREIGENHEAAARIQLKLIESQPWEDSLKDGHHLKQLLLKALTLMLDAAESYAKDSCVRQAQHCHRLTKLITLQIHFLNTGQNTMLINLGRHRLMDCILALPRFYQASIVAEAYDFVPDWAEILYQQVILKGDFNYLEEFKQQRLLRSSIFEEISKKYKQHQPTDTAVKNLKKLLTYCEDVYLYYKLAYEHKFYDIVNVLLKDPQSGCCLKDMLAG

Target ORF information:

RefSeq Version	XM_003640025.3
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris spastic paraplegia 11 (autosomal recessive) (SPG11), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_003640025.3

ORF Insert Sequence:

ATGGCCGCAG GAGAGGCAGC TGCGAGAGCT GCTGGTTGCA GCAGCTGCGG CGCTGAAACT 
ATGGAACGGG TTCTACCTAT GCTGTTGGTG CCGGTCCCTG TGGAGGCAAC CGGGCGGTTG 
GGCTCCCGGG CGCAGTTGCA CAGAGAGCAG GAGGTTCTGG GGAGCCTAAC AGCTGCAGGC 
AGCCTGCAGG TGCTTTCCTT GGCGCCGGGC GGCCGGAGCG GCGGGGCCTG CTGCCTCAAT 
GGCCCCTTTC GGCACTTTAT ATGGGAGGAT TCTCATAACG GCAGCTCACC AACAGACAAG 
CCCAAACTTC TTGCTCTTAG TGAAAACTAT GAACTGTTTA TCTATGAATT TAATTTGAAA 
GATGGAAGAT GTGATGCAAC CATTTTGTAT AGCTGTAGTG AGGAGACATT GCAGAAGCTC 
ATTGAAGATC AAAATATCAG TGTTTCCTTT TTATCTTTGA GAATTCTGTC ATTTCACAAT 
AACACATCAT TATTGTTCAT CAATAAATGT ATTGTCCTAC ACATTATATT TCCTGAAAGA 
GATGCTGAGA TCAGAGTACT CAACTGTTTC ACGTTACCCT TGCCTGCACA GGCAGTGGAC 
AGGATCATTG ATGTGCAGCT CTGCAGAGGA ATTCTTTTTG TTTTGAGTAG TTTAGGCTGG 
ATCTACATTT TTGACATTGT GGATGGAAGA CATGTAGCTC ACGTGGATTT AGCACTTCAC 
CAAGAAGATA TGTGTAATGA ACAGCAACAG GAACTAGCCA AGATTTCTTC CTTTACTTCA 
CTGAAAGTGT CTCAAGACCT AGATGTTGTA GTGATTGTCA GCTCCTTCAA CTCTGCAGTT 
GCTCTTAACC TAAACTTGTA TTTCAGGCAA CACCCAGGAC ACCTACTGTG TGAAAGAACT 
CTAGAAGACA TTCCTATTGA AGGCCCTGAG GGGATCGATG AAGATGATCC TGTTAATTCT 
GACTACAACA TGAAACTGAC CAAGTTTTCC TTTCAAGTTG ATAGGTCTTG GAAAGCCCAG 
CTATCATCAC TGAATGAAAT AATAAAGAGC TCCAAATGGG AGGTTCCCTG TTGTGCTCCA 
TGGTTCCAGA ATATTTTGCA TTTGGAGTCA CCGGAATCTG GTTACCACAG TACTAGTACA 
CCAGGCTGGG CCTTCATCCC ACAGGATGAA ATTCATAGCC ACTACAAGTT TCCACAGAAA 
GATCATGCCA ACACCGGTGA CCCAGGAAGA TCATGGAAAA TAATGAACAT TAGTGAACTA 
AAGGAACCTG TTGAGCTTAC ATGTATGTCT GTGACAGGAT TCAGTGTACT GTTTACTTGG 
GCAGTGGAAA AGATGGGCTG CACCATTGTC CTTTGGGATT TGGAGACCCA GGGTATCCAG 
TGTTTTTCCC TTGGCAAAAG GTGTATTCCT ATAGATATGA GTGGAGACCA GCAGCTGTGC 
CTTGTTTTGA CAGAAAATGG ACTCTCCCTG ATTTTGTTTG GATTGACTCA GGAGGAGTTT 
TTAAATAGAC TAATGATCCA TGGAAGTGCC AGCACTGTGG ACTCTCTTTG CCATCTCAAT 
GGTTGGGGGA GGTGCTCAAT CCCCATACAT GCGCTGGAGG CTGGGATAGA AAATCGTCAG 
CTGGACACCG TAGATTTCTT TTTGAAGAGC AAAGAAAATC TTTTGAATCC ATCCTCAAAA 
TCTTCCGTAC CGGATCAGTT TGATGACTTT CCATCTCATT TATATTTAAG AAATGTGGAA 
AAGCTGACAC CAGCATTGGA TTTACTTTGC TCAGCAATTA GAGAAAGTGA TTCTGAAACC 
CAAAGCAAAC ACTTTTCAGA ACAGTTGCTT AACCTTACAC TGTCTTTTCT TAACAAACAA 
ATAAAGGAGC TTTTTGTTCA CAATGAAGAA TTAGATGAAC ATCTACAGAA AGGAGTGAAC 
ATTTTGACCA GCTACATTAA TGAACTTCGA ACCTTCATGA TAAAATTTCC TTGGAAGATA 
GTAGACCCAG TAGATGAATA TGATGTAAGT GAAAATGTCC TCAAAGTAAG AGAGAGTGAT 
ACATGGGAGA AACTCAGCAT TGAGGAAGTT ATTGCCAATG CTATTTTAAA CAACAAAATA 
CCAGAGGCAC AGACTTTCTT CAGGATTAAC AATCATTCTG CTCAAAGACT TGAGGAGCTG 
ATTAGAATAG GCCTAAATTT GGTCTTTGAC TGTTTGAAAA AGAACAATAT AAAGGAAGCA 
TCTGAACTTT TGAAGAATAT GGGCTTTGAT GTAAAAGACC AGTTGCTCAA GATATGTTTC 
TATACAACTG ATAAAAATAT ACGGGACTTT TTGGTGGAAA TTTTAAAAGA AAAAAATTAT 
TTTTCTGAAA ATGAAAAAAG AACTATAGAC TTCGTGCATC AAGTTGAGAA GTTTTATTCA 
GGACATTTTC AAGAAAATGT GCAGATCCAG TCCTTTACCA GGTATTGGAT AAAGGAACAG 
GATTTTTTCA AGCACAAATC TGTTTTGGAC TCACTCCTGA AATATGATCC TAAAGATAAA 
TTTAACAAAC AGGACCATAG AATTGTGTTA AATTGGGCTC AGTGGTGGAA TCAACTAACA 
CAAGAATCCA TCCTTCTCCC CAGGATAAGT CCAGAAGAAT ACAAATCATG TTCTCCTGAA 
GCCCTTTGGA GCTATCTCAC AGCCCATCAT GATTGGTCAA GCATTAGCTT GTGGATTGAA 
GAATTTCAGG CCCAGAACAG TTGTGTTTTA CTGCAGCAGA ACAAATGGCC CCTTCTGACT 
GTTGATGTTA TAGACCAGAA TACTTGCTGC AACAACTACA TGAGGAATGA AATTTTAGAT 
CAGCTGGCCA GGAATGGGAT TTTTCTTCAA TCTGAGCTAG AAGACTTTGA ACTCTTCCTG 
CTAAGACTGA ATCGCATTGG GGGTGTGATG CAAGATGCTC TCCCCATTCA AAACTACAGA 
TCCAAAGAAG GTTGGGATTT CCATTCTCAC TTCATTCTCT ATTGTTTGGA ACATGGTCTG 
CAGTATCCTC TCTATGTCTA TCTTGACTTT TACAAACTTA GTCCTGCAAA TTGTCCCTTT 
TTGGAAAAAA AAGAATTACA TGAAACTCAC CCTTGGTTTG AATTTTTGGT TCAGTGTCGA 
CAGGTTTCCA GTAATTTAAC AGATCCCAAA CTGATCTTCC AGGCTAGCCT TGCAAACGCT 
CAGATACTGA TTCCCAGCAA CCAGGCCAGT GTAAGCAGTA TGCTATTGGA AGGACATACT 
CTCCTGGCCC TTGCTACCAC AATGTATGCC CCTGGCGGTG TCAGCCAGGT TGTTCAGGAT 
GATGAAAATG AGACCTGTTT GAAGAAAGTA GATCCCCAGT TACTGAAGAT GGCATTAACT 
CCTTATCCCA AGCTAAAAAC TGCTCTCTTC CCACAGTACA CTACCTCTAA TGTTCTGCCG 
CCTGATATTA CGCTTTACCA CCTTATTCAG TCACTATTGC CCTTTGACTC TAGCAGATTG 
TTTGGCTGGC AGTCTGCCAA CACACTGGCT ATTGGAGATG CAGTGAGTCA TCTCCCACAT 
TTTTCTAGCC CTGATCTGGT TAATAAATAT GCTATCATGG AACGTCTGAA TTTTGCATAT 
TATTTACAAC ATGGACGGCC GTCATTTGCA TTTGGTACTT TTCTGGTCCA GGAATTAACC 
AAGAGCAAGA CTCCCAAGCA GCTGATCCAG CAAGTAGGCA ATGAAGCTTA TGTTTTAGGG 
CTCTCCTCCT TCTACATTCC ATCAGTAGGA GCTGCCTGTG TTTGTTTCTT AGAACTGCTT 
GGCCTCGATA GCCTCAAGCT CAGAGTTGAC ATGAAAGTGG CCAATATAAT TTTGAACTAC 
AAATGTAGAA ATGAAGATGT TCAGTACAGC TGTATCAGAG AGGCTCTAGT TGAAAAACTA 
TCTAAACTGG CTGTGGGTGA AAAGGCCACC GTAGAGGAAT TGCTTATTCT CTTAGAAGAA 
GGTATATGGA ACAACATTCA GCAGCAAGAG ATAAAGAGGT TATCTAGTGA ATCTAGCAGC 
CAGTGGGCAT TAGCAGTACA GTTCTGCAGG CTTCACAATG TGAAGCTGAG CACGTCTTAC 
CTTAGAGAAT GTGCCAAAGC AAATGACTGG TTACAGTTCA TTATTCACAG CCAACTCTAT 
AACTATCACC CAGCGGAGGT GAAATCTCTT CTTCAGTATT TCAGTCCTGT CCTTCAAGAC 
CACTTAAGAC TAGCTTTTGA GAACCTGACC TCAGTGTCTA AATCCAAAAT GGATAGTGGT 
GAAGTCTGCA GCAGGTCTCC CCAGGAACTC CAGAGAAACA AAGAAGAAAT GGCTGATTTC 
TTTCAAGTTC TACTCCAGTG CTCAGAGGAA CCAAACCCCT GGTGCTGGCT CCTAGCTGAA 
GCAATGAAAC AACAGGCTCC TATCCTTAGT GTCCTGGCCT CATGTCTTCA GGATGCCAGT 
ACTATTCCTT GTCTCTGTGT TTGGATCATC ACATCTGTGG AGGACAGTGT TGCAGCTGAA 
GCAACAGAAC ACATTCAGGG CTCAGTGGAA GGCCACATAT GGGACCTTCA AGACCTCTCA 
GTCATCTGGA GAACATTATT AACAAGGCAG AAGAACAAAA CTCTCATCAG AGGTTTCCAA 
CTTTTCTTTA AGGATTCCCC ACTACTGTTG ATGATGGAGA TGTATGAACT GTGTATGTTC 
TTCAAGAATT ATAAAGAAGC TGAAGCTAAA CTTCTGGAGT TCCAAAAGAG CTTTGAAACT 
GTTGAAACAG CAGCCACAAG GGTCCACACT ACCATCCCTG CCCCATGGCT GAGGGAGCAG 
GTGTGTTTTC TTCTGAAGCT TATGCTGCAG CAGTGTGGGA CCCAGTATGA ACTGGGGAAG 
CTTTTACAGC TATTTGTTGG AATAGAAAAT TTCTTCTCTG ATGGTCCAGA TGTGAAGAAG 
CTCTGTGTCC TTAGTGAAAT TTTGAAGGAC ACATCCATAG CCATTAATCA TGTAATTATT 
AGCAGCCACA GCCTTGAGAA TTTTCAGCAT GAATGTACAT CTATTTTGGA AAAACTGGCG 
ATGGAGGGAC AGTTTGCTTT GGCCAGGAGA GTAGCAGAAT TAGCTGAATT ACCTGTGGAC 
AACTTGGTTA TTGAAGAGAT GACACAGGAA ATGCAAACCT TAAAACACAT TGACCAGTGG 
TCCCTGAAAC AAGCAAGAAT TGACTTCTGG AAAAAATGTC ATGAGAATTT TAAGAAAAAT 
TCCATTTCAA GCAAAGCAGC TTCTTCCTTT TTCTCAGCCC AGGCACCTGA GTGCCCAACT 
GAAGAGGGTC GGAGCAGCAG TGAGGAGCGC CATCTGCTGC TCACATTGGC AGCACACTGG 
CTTGCTCAGG AGGATCTGGT GCCGGTGGCC GAACTGGAGG AGCTAGAGAA GCAGATCTGG 
CTCTGCCGCA TTACCCAATA CACCCTCAGA GAAAAACAGG AGCAAGTAGA GCTCGGGTTT 
TCTCAACAGA TTTCAACTAG TGGGGAACTT TCCTTTGATG GCATAGCCAG TAAGTTTTCA 
CTCTCCATGT TGGCTGTTCT GAACACATCA AAGTACTTAG AACTCAGTGG CTTTCCATCC 
AAAGAGACAT GTGATAGCGG ACTGGATTGG AAGGAGCAGG AGTCACTGAA CTTTCTGATT 
GGGCACCTAT TGGATGATGG CTTTGTGCAT GAAGCAAGTA GAGTGTGTCG GTATTTTCAT 
TTCTATAACC AAGATGTTGC CTTGGTGTTG CACTGCAGAG CACTGGCCTC AGGGGAAGCT 
AGTGTGGATG ACCTGCACCC AGAGATCCAT GCTCTCCTAC AAAGTGCTGA GCCGCTTGAG 
GAGGAGGAAG CTGGCATTCC ACTAAGAAAA GTCCAGAGCA GTTCAAGTTT GGATAGTCAG 
TCATTTGTGA TGCTGCCCCC CAGTGATGAA GTGGTGACTA ATCTGGAAAC TCTGACAAGT 
AAATGCCTCC ATGGGAAGAA CTACTGTCGG CAGGTCCTGT GTCTGTATGA ACTTGCCAAG 
GAGTTGGGCT GTTCCTACAC AGACGTGGCC TCTCAGGACG GGGAGGCCAT GCTCCGGGCA 
ATCTTGGCTT CCCAGCAGCC CGACCGATGC AAGCGAGCCC AGGCTTTCAT TAGCACCCAG 
GGCCTCGAGG CAGACACGGT GGCTGAACTT GTGGCTGAAG AGGTGACCCG GGAGCTGCTG 
ACCCCACCGG AGGGAACAGG ACATCAGCAG ATGTTTGCCC CAGCAGAGGA AAGCCGGACA 
TTTCTTCAGC TGACCACTCT GTGTCAAGAC CGCACACTGG TAGGCATGAA GTTGTTGGAC 
AAGATTTCGT CCGTTCCCCA TGGAGAATTA TCTTGCACCA CAGAGCTGCT GATCCTGGCC 
CATCACTGCT TCACACTGAC ATGCCACATG GAGGGCATCA TCCGAGTCCT GCAAGCTGCC 
CGGCTGCTCA CGGACAAACA CTTGGCCCCC AATGAAGAGT ACGGGCTGAT GGTACGCCTC 
CTCACCGGCA TCGGAAGGTA CAACGAGATG ACGTACATAT TTGACTTGCT GCATAATAAG 
CACTATTTTG AAGTGCTGAT GAGGAAGAAG TTGGATCCGA GTGGCTCCCT GAAGACCGCC 
CTACTGGACT ACATCAAACG CTGCCGCCCT GGGGACAGTG AGAAGCACAA CATGATCGCT 
CTGTGCTTCA GCATGTGTCG GGAGATTGGG GAGAACCATG AGGCAGCTGC CCGCATCCAG 
CTGAAACTGA TCGAGTCTCA GCCCTGGGAG GACAGCCTCA AGGACGGGCA CCACCTGAAG 
CAGCTGCTGC TGAAAGCCCT GACTCTGATG CTGGATGCAG CCGAGAGCTA TGCCAAGGAC 
TCCTGTGTGC GACAGGCCCA GCACTGTCAC CGGCTCACCA AGTTGATAAC ACTGCAGATT 
CATTTTCTGA ACACTGGCCA GAACACAATG CTCATCAACC TGGGCCGCCA CCGGCTGATG 
GACTGTATCC TCGCTCTACC TCGGTTCTAC CAGGCTTCTA TTGTGGCCGA AGCCTATGAC 
TTTGTTCCTG ATTGGGCAGA AATCCTCTAC CAACAAGTGA TTCTTAAAGG AGACTTTAAT 
TACCTGGAAG AATTTAAGCA GCAAAGGTTA TTAAGATCCA GTATATTTGA AGAGATTTCA 
AAAAAATATA AACAACATCA GCCGACAGAC ACGGCTGTGA AAAACCTGAA GAAGTTACTC 
ACATACTGTG AAGATGTCTA CTTGTACTAC AAGCTCGCAT ATGAACACAA ATTTTATGAC 
ATTGTGAATG TGCTTCTGAA GGACCCTCAG AGCGGCTGCT GTCTAAAGGA CATGCTAGCA 
GGTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SPG11 ( XM_003640025.4 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_003640073.2 Canis lupus familiaris SPG11, spatacsin vesicle trafficking associated (SPG11), transcript variant X1, mRNA.

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CloneID	OCe28791
Clone ID Related Accession (Same CDS sequence)	XM_003640025.3 , XM_003640025.4
Accession Version	XM_003640025.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7326bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1504454400000
Organism	Canis lupus familiaris(dog)
Product	spatacsin isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006612.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_003640025.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCGCAG GAGAGGCAGC TGCGAGAGCT GCTGGTTGCA GCAGCTGCGG CGCTGAAACT 
ATGGAACGGG TTCTACCTAT GCTGTTGGTG CCGGTCCCTG TGGAGGCAAC CGGGCGGTTG 
GGCTCCCGGG CGCAGTTGCA CAGAGAGCAG GAGGTTCTGG GGAGCCTAAC AGCTGCAGGC 
AGCCTGCAGG TGCTTTCCTT GGCGCCGGGC GGCCGGAGCG GCGGGGCCTG CTGCCTCAAT 
GGCCCCTTTC GGCACTTTAT ATGGGAGGAT TCTCATAACG GCAGCTCACC AACAGACAAG 
CCCAAACTTC TTGCTCTTAG TGAAAACTAT GAACTGTTTA TCTATGAATT TAATTTGAAA 
GATGGAAGAT GTGATGCAAC CATTTTGTAT AGCTGTAGTG AGGAGACATT GCAGAAGCTC 
ATTGAAGATC AAAATATCAG TGTTTCCTTT TTATCTTTGA GAATTCTGTC ATTTCACAAT 
AACACATCAT TATTGTTCAT CAATAAATGT ATTGTCCTAC ACATTATATT TCCTGAAAGA 
GATGCTGAGA TCAGAGTACT CAACTGTTTC ACGTTACCCT TGCCTGCACA GGCAGTGGAC 
AGGATCATTG ATGTGCAGCT CTGCAGAGGA ATTCTTTTTG TTTTGAGTAG TTTAGGCTGG 
ATCTACATTT TTGACATTGT GGATGGAAGA CATGTAGCTC ACGTGGATTT AGCACTTCAC 
CAAGAAGATA TGTGTAATGA ACAGCAACAG GAACTAGCCA AGATTTCTTC CTTTACTTCA 
CTGAAAGTGT CTCAAGACCT AGATGTTGTA GTGATTGTCA GCTCCTTCAA CTCTGCAGTT 
GCTCTTAACC TAAACTTGTA TTTCAGGCAA CACCCAGGAC ACCTACTGTG TGAAAGAACT 
CTAGAAGACA TTCCTATTGA AGGCCCTGAG GGGATCGATG AAGATGATCC TGTTAATTCT 
GACTACAACA TGAAACTGAC CAAGTTTTCC TTTCAAGTTG ATAGGTCTTG GAAAGCCCAG 
CTATCATCAC TGAATGAAAT AATAAAGAGC TCCAAATGGG AGGTTCCCTG TTGTGCTCCA 
TGGTTCCAGA ATATTTTGCA TTTGGAGTCA CCGGAATCTG GTTACCACAG TACTAGTACA 
CCAGGCTGGG CCTTCATCCC ACAGGATGAA ATTCATAGCC ACTACAAGTT TCCACAGAAA 
GATCATGCCA ACACCGGTGA CCCAGGAAGA TCATGGAAAA TAATGAACAT TAGTGAACTA 
AAGGAACCTG TTGAGCTTAC ATGTATGTCT GTGACAGGAT TCAGTGTACT GTTTACTTGG 
GCAGTGGAAA AGATGGGCTG CACCATTGTC CTTTGGGATT TGGAGACCCA GGGTATCCAG 
TGTTTTTCCC TTGGCAAAAG GTGTATTCCT ATAGATATGA GTGGAGACCA GCAGCTGTGC 
CTTGTTTTGA CAGAAAATGG ACTCTCCCTG ATTTTGTTTG GATTGACTCA GGAGGAGTTT 
TTAAATAGAC TAATGATCCA TGGAAGTGCC AGCACTGTGG ACTCTCTTTG CCATCTCAAT 
GGTTGGGGGA GGTGCTCAAT CCCCATACAT GCGCTGGAGG CTGGGATAGA AAATCGTCAG 
CTGGACACCG TAGATTTCTT TTTGAAGAGC AAAGAAAATC TTTTGAATCC ATCCTCAAAA 
TCTTCCGTAC CGGATCAGTT TGATGACTTT CCATCTCATT TATATTTAAG AAATGTGGAA 
AAGCTGACAC CAGCATTGGA TTTACTTTGC TCAGCAATTA GAGAAAGTGA TTCTGAAACC 
CAAAGCAAAC ACTTTTCAGA ACAGTTGCTT AACCTTACAC TGTCTTTTCT TAACAAACAA 
ATAAAGGAGC TTTTTGTTCA CAATGAAGAA TTAGATGAAC ATCTACAGAA AGGAGTGAAC 
ATTTTGACCA GCTACATTAA TGAACTTCGA ACCTTCATGA TAAAATTTCC TTGGAAGATA 
GTAGACCCAG TAGATGAATA TGATGTAAGT GAAAATGTCC TCAAAGTAAG AGAGAGTGAT 
ACATGGGAGA AACTCAGCAT TGAGGAAGTT ATTGCCAATG CTATTTTAAA CAACAAAATA 
CCAGAGGCAC AGACTTTCTT CAGGATTAAC AATCATTCTG CTCAAAGACT TGAGGAGCTG 
ATTAGAATAG GCCTAAATTT GGTCTTTGAC TGTTTGAAAA AGAACAATAT AAAGGAAGCA 
TCTGAACTTT TGAAGAATAT GGGCTTTGAT GTAAAAGACC AGTTGCTCAA GATATGTTTC 
TATACAACTG ATAAAAATAT ACGGGACTTT TTGGTGGAAA TTTTAAAAGA AAAAAATTAT 
TTTTCTGAAA ATGAAAAAAG AACTATAGAC TTCGTGCATC AAGTTGAGAA GTTTTATTCA 
GGACATTTTC AAGAAAATGT GCAGATCCAG TCCTTTACCA GGTATTGGAT AAAGGAACAG 
GATTTTTTCA AGCACAAATC TGTTTTGGAC TCACTCCTGA AATATGATCC TAAAGATAAA 
TTTAACAAAC AGGACCATAG AATTGTGTTA AATTGGGCTC AGTGGTGGAA TCAACTAACA 
CAAGAATCCA TCCTTCTCCC CAGGATAAGT CCAGAAGAAT ACAAATCATG TTCTCCTGAA 
GCCCTTTGGA GCTATCTCAC AGCCCATCAT GATTGGTCAA GCATTAGCTT GTGGATTGAA 
GAATTTCAGG CCCAGAACAG TTGTGTTTTA CTGCAGCAGA ACAAATGGCC CCTTCTGACT 
GTTGATGTTA TAGACCAGAA TACTTGCTGC AACAACTACA TGAGGAATGA AATTTTAGAT 
CAGCTGGCCA GGAATGGGAT TTTTCTTCAA TCTGAGCTAG AAGACTTTGA ACTCTTCCTG 
CTAAGACTGA ATCGCATTGG GGGTGTGATG CAAGATGCTC TCCCCATTCA AAACTACAGA 
TCCAAAGAAG GTTGGGATTT CCATTCTCAC TTCATTCTCT ATTGTTTGGA ACATGGTCTG 
CAGTATCCTC TCTATGTCTA TCTTGACTTT TACAAACTTA GTCCTGCAAA TTGTCCCTTT 
TTGGAAAAAA AAGAATTACA TGAAACTCAC CCTTGGTTTG AATTTTTGGT TCAGTGTCGA 
CAGGTTTCCA GTAATTTAAC AGATCCCAAA CTGATCTTCC AGGCTAGCCT TGCAAACGCT 
CAGATACTGA TTCCCAGCAA CCAGGCCAGT GTAAGCAGTA TGCTATTGGA AGGACATACT 
CTCCTGGCCC TTGCTACCAC AATGTATGCC CCTGGCGGTG TCAGCCAGGT TGTTCAGGAT 
GATGAAAATG AGACCTGTTT GAAGAAAGTA GATCCCCAGT TACTGAAGAT GGCATTAACT 
CCTTATCCCA AGCTAAAAAC TGCTCTCTTC CCACAGTACA CTACCTCTAA TGTTCTGCCG 
CCTGATATTA CGCTTTACCA CCTTATTCAG TCACTATTGC CCTTTGACTC TAGCAGATTG 
TTTGGCTGGC AGTCTGCCAA CACACTGGCT ATTGGAGATG CAGTGAGTCA TCTCCCACAT 
TTTTCTAGCC CTGATCTGGT TAATAAATAT GCTATCATGG AACGTCTGAA TTTTGCATAT 
TATTTACAAC ATGGACGGCC GTCATTTGCA TTTGGTACTT TTCTGGTCCA GGAATTAACC 
AAGAGCAAGA CTCCCAAGCA GCTGATCCAG CAAGTAGGCA ATGAAGCTTA TGTTTTAGGG 
CTCTCCTCCT TCTACATTCC ATCAGTAGGA GCTGCCTGTG TTTGTTTCTT AGAACTGCTT 
GGCCTCGATA GCCTCAAGCT CAGAGTTGAC ATGAAAGTGG CCAATATAAT TTTGAACTAC 
AAATGTAGAA ATGAAGATGT TCAGTACAGC TGTATCAGAG AGGCTCTAGT TGAAAAACTA 
TCTAAACTGG CTGTGGGTGA AAAGGCCACC GTAGAGGAAT TGCTTATTCT CTTAGAAGAA 
GGTATATGGA ACAACATTCA GCAGCAAGAG ATAAAGAGGT TATCTAGTGA ATCTAGCAGC 
CAGTGGGCAT TAGCAGTACA GTTCTGCAGG CTTCACAATG TGAAGCTGAG CACGTCTTAC 
CTTAGAGAAT GTGCCAAAGC AAATGACTGG TTACAGTTCA TTATTCACAG CCAACTCTAT 
AACTATCACC CAGCGGAGGT GAAATCTCTT CTTCAGTATT TCAGTCCTGT CCTTCAAGAC 
CACTTAAGAC TAGCTTTTGA GAACCTGACC TCAGTGTCTA AATCCAAAAT GGATAGTGGT 
GAAGTCTGCA GCAGGTCTCC CCAGGAACTC CAGAGAAACA AAGAAGAAAT GGCTGATTTC 
TTTCAAGTTC TACTCCAGTG CTCAGAGGAA CCAAACCCCT GGTGCTGGCT CCTAGCTGAA 
GCAATGAAAC AACAGGCTCC TATCCTTAGT GTCCTGGCCT CATGTCTTCA GGATGCCAGT 
ACTATTCCTT GTCTCTGTGT TTGGATCATC ACATCTGTGG AGGACAGTGT TGCAGCTGAA 
GCAACAGAAC ACATTCAGGG CTCAGTGGAA GGCCACATAT GGGACCTTCA AGACCTCTCA 
GTCATCTGGA GAACATTATT AACAAGGCAG AAGAACAAAA CTCTCATCAG AGGTTTCCAA 
CTTTTCTTTA AGGATTCCCC ACTACTGTTG ATGATGGAGA TGTATGAACT GTGTATGTTC 
TTCAAGAATT ATAAAGAAGC TGAAGCTAAA CTTCTGGAGT TCCAAAAGAG CTTTGAAACT 
GTTGAAACAG CAGCCACAAG GGTCCACACT ACCATCCCTG CCCCATGGCT GAGGGAGCAG 
GTGTGTTTTC TTCTGAAGCT TATGCTGCAG CAGTGTGGGA CCCAGTATGA ACTGGGGAAG 
CTTTTACAGC TATTTGTTGG AATAGAAAAT TTCTTCTCTG ATGGTCCAGA TGTGAAGAAG 
CTCTGTGTCC TTAGTGAAAT TTTGAAGGAC ACATCCATAG CCATTAATCA TGTAATTATT 
AGCAGCCACA GCCTTGAGAA TTTTCAGCAT GAATGTACAT CTATTTTGGA AAAACTGGCG 
ATGGAGGGAC AGTTTGCTTT GGCCAGGAGA GTAGCAGAAT TAGCTGAATT ACCTGTGGAC 
AACTTGGTTA TTGAAGAGAT GACACAGGAA ATGCAAACCT TAAAACACAT TGACCAGTGG 
TCCCTGAAAC AAGCAAGAAT TGACTTCTGG AAAAAATGTC ATGAGAATTT TAAGAAAAAT 
TCCATTTCAA GCAAAGCAGC TTCTTCCTTT TTCTCAGCCC AGGCACCTGA GTGCCCAACT 
GAAGAGGGTC GGAGCAGCAG TGAGGAGCGC CATCTGCTGC TCACATTGGC AGCACACTGG 
CTTGCTCAGG AGGATCTGGT GCCGGTGGCC GAACTGGAGG AGCTAGAGAA GCAGATCTGG 
CTCTGCCGCA TTACCCAATA CACCCTCAGA GAAAAACAGG AGCAAGTAGA GCTCGGGTTT 
TCTCAACAGA TTTCAACTAG TGGGGAACTT TCCTTTGATG GCATAGCCAG TAAGTTTTCA 
CTCTCCATGT TGGCTGTTCT GAACACATCA AAGTACTTAG AACTCAGTGG CTTTCCATCC 
AAAGAGACAT GTGATAGCGG ACTGGATTGG AAGGAGCAGG AGTCACTGAA CTTTCTGATT 
GGGCACCTAT TGGATGATGG CTTTGTGCAT GAAGCAAGTA GAGTGTGTCG GTATTTTCAT 
TTCTATAACC AAGATGTTGC CTTGGTGTTG CACTGCAGAG CACTGGCCTC AGGGGAAGCT 
AGTGTGGATG ACCTGCACCC AGAGATCCAT GCTCTCCTAC AAAGTGCTGA GCCGCTTGAG 
GAGGAGGAAG CTGGCATTCC ACTAAGAAAA GTCCAGAGCA GTTCAAGTTT GGATAGTCAG 
TCATTTGTGA TGCTGCCCCC CAGTGATGAA GTGGTGACTA ATCTGGAAAC TCTGACAAGT 
AAATGCCTCC ATGGGAAGAA CTACTGTCGG CAGGTCCTGT GTCTGTATGA ACTTGCCAAG 
GAGTTGGGCT GTTCCTACAC AGACGTGGCC TCTCAGGACG GGGAGGCCAT GCTCCGGGCA 
ATCTTGGCTT CCCAGCAGCC CGACCGATGC AAGCGAGCCC AGGCTTTCAT TAGCACCCAG 
GGCCTCGAGG CAGACACGGT GGCTGAACTT GTGGCTGAAG AGGTGACCCG GGAGCTGCTG 
ACCCCACCGG AGGGAACAGG ACATCAGCAG ATGTTTGCCC CAGCAGAGGA AAGCCGGACA 
TTTCTTCAGC TGACCACTCT GTGTCAAGAC CGCACACTGG TAGGCATGAA GTTGTTGGAC 
AAGATTTCGT CCGTTCCCCA TGGAGAATTA TCTTGCACCA CAGAGCTGCT GATCCTGGCC 
CATCACTGCT TCACACTGAC ATGCCACATG GAGGGCATCA TCCGAGTCCT GCAAGCTGCC 
CGGCTGCTCA CGGACAAACA CTTGGCCCCC AATGAAGAGT ACGGGCTGAT GGTACGCCTC 
CTCACCGGCA TCGGAAGGTA CAACGAGATG ACGTACATAT TTGACTTGCT GCATAATAAG 
CACTATTTTG AAGTGCTGAT GAGGAAGAAG TTGGATCCGA GTGGCTCCCT GAAGACCGCC 
CTACTGGACT ACATCAAACG CTGCCGCCCT GGGGACAGTG AGAAGCACAA CATGATCGCT 
CTGTGCTTCA GCATGTGTCG GGAGATTGGG GAGAACCATG AGGCAGCTGC CCGCATCCAG 
CTGAAACTGA TCGAGTCTCA GCCCTGGGAG GACAGCCTCA AGGACGGGCA CCACCTGAAG 
CAGCTGCTGC TGAAAGCCCT GACTCTGATG CTGGATGCAG CCGAGAGCTA TGCCAAGGAC 
TCCTGTGTGC GACAGGCCCA GCACTGTCAC CGGCTCACCA AGTTGATAAC ACTGCAGATT 
CATTTTCTGA ACACTGGCCA GAACACAATG CTCATCAACC TGGGCCGCCA CCGGCTGATG 
GACTGTATCC TCGCTCTACC TCGGTTCTAC CAGGCTTCTA TTGTGGCCGA AGCCTATGAC 
TTTGTTCCTG ATTGGGCAGA AATCCTCTAC CAACAAGTGA TTCTTAAAGG AGACTTTAAT 
TACCTGGAAG AATTTAAGCA GCAAAGGTTA TTAAGATCCA GTATATTTGA AGAGATTTCA 
AAAAAATATA AACAACATCA GCCGACAGAC ACGGCTGTGA AAAACCTGAA GAAGTTACTC 
ACATACTGTG AAGATGTCTA CTTGTACTAC AAGCTCGCAT ATGAACACAA ATTTTATGAC 
ATTGTGAATG TGCTTCTGAA GGACCCTCAG AGCGGCTGCT GTCTAAAGGA CATGCTAGCA 
GGTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_003640073.2
CDS	21..7346
Translation	MAAGEAAARAAGCSSCGAETMERVLPMLLVPVPVEATGRLGSRAQLHREQEVLGSLTAAGSLQVLSLAPGGRSGGACCLNGPFRHFIWEDSHNGSSPTDKPKLLALSENYELFIYEFNLKDGRCDATILYSCSEETLQKLIEDQNISVSFLSLRILSFHNNTSLLFINKCIVLHIIFPERDAEIRVLNCFTLPLPAQAVDRIIDVQLCRGILFVLSSLGWIYIFDIVDGRHVAHVDLALHQEDMCNEQQQELAKISSFTSLKVSQDLDVVVIVSSFNSAVALNLNLYFRQHPGHLLCERTLEDIPIEGPEGIDEDDPVNSDYNMKLTKFSFQVDRSWKAQLSSLNEIIKSSKWEVPCCAPWFQNILHLESPESGYHSTSTPGWAFIPQDEIHSHYKFPQKDHANTGDPGRSWKIMNISELKEPVELTCMSVTGFSVLFTWAVEKMGCTIVLWDLETQGIQCFSLGKRCIPIDMSGDQQLCLVLTENGLSLILFGLTQEEFLNRLMIHGSASTVDSLCHLNGWGRCSIPIHALEAGIENRQLDTVDFFLKSKENLLNPSSKSSVPDQFDDFPSHLYLRNVEKLTPALDLLCSAIRESDSETQSKHFSEQLLNLTLSFLNKQIKELFVHNEELDEHLQKGVNILTSYINELRTFMIKFPWKIVDPVDEYDVSENVLKVRESDTWEKLSIEEVIANAILNNKIPEAQTFFRINNHSAQRLEELIRIGLNLVFDCLKKNNIKEASELLKNMGFDVKDQLLKICFYTTDKNIRDFLVEILKEKNYFSENEKRTIDFVHQVEKFYSGHFQENVQIQSFTRYWIKEQDFFKHKSVLDSLLKYDPKDKFNKQDHRIVLNWAQWWNQLTQESILLPRISPEEYKSCSPEALWSYLTAHHDWSSISLWIEEFQAQNSCVLLQQNKWPLLTVDVIDQNTCCNNYMRNEILDQLARNGIFLQSELEDFELFLLRLNRIGGVMQDALPIQNYRSKEGWDFHSHFILYCLEHGLQYPLYVYLDFYKLSPANCPFLEKKELHETHPWFEFLVQCRQVSSNLTDPKLIFQASLANAQILIPSNQASVSSMLLEGHTLLALATTMYAPGGVSQVVQDDENETCLKKVDPQLLKMALTPYPKLKTALFPQYTTSNVLPPDITLYHLIQSLLPFDSSRLFGWQSANTLAIGDAVSHLPHFSSPDLVNKYAIMERLNFAYYLQHGRPSFAFGTFLVQELTKSKTPKQLIQQVGNEAYVLGLSSFYIPSVGAACVCFLELLGLDSLKLRVDMKVANIILNYKCRNEDVQYSCIREALVEKLSKLAVGEKATVEELLILLEEGIWNNIQQQEIKRLSSESSSQWALAVQFCRLHNVKLSTSYLRECAKANDWLQFIIHSQLYNYHPAEVKSLLQYFSPVLQDHLRLAFENLTSVSKSKMDSGEVCSRSPQELQRNKEEMADFFQVLLQCSEEPNPWCWLLAEAMKQQAPILSVLASCLQDASTIPCLCVWIITSVEDSVAAEATEHIQGSVEGHIWDLQDLSVIWRTLLTRQKNKTLIRGFQLFFKDSPLLLMMEMYELCMFFKNYKEAEAKLLEFQKSFETVETAATRVHTTIPAPWLREQVCFLLKLMLQQCGTQYELGKLLQLFVGIENFFSDGPDVKKLCVLSEILKDTSIAINHVIISSHSLENFQHECTSILEKLAMEGQFALARRVAELAELPVDNLVIEEMTQEMQTLKHIDQWSLKQARIDFWKKCHENFKKNSISSKAASSFFSAQAPECPTEEGRSSSEERHLLLTLAAHWLAQEDLVPVAELEELEKQIWLCRITQYTLREKQEQVELGFSQQISTSGELSFDGIASKFSLSMLAVLNTSKYLELSGFPSKETCDSGLDWKEQESLNFLIGHLLDDGFVHEASRVCRYFHFYNQDVALVLHCRALASGEASVDDLHPEIHALLQSAEPLEEEEAGIPLRKVQSSSSLDSQSFVMLPPSDEVVTNLETLTSKCLHGKNYCRQVLCLYELAKELGCSYTDVASQDGEAMLRAILASQQPDRCKRAQAFISTQGLEADTVAELVAEEVTRELLTPPEGTGHQQMFAPAEESRTFLQLTTLCQDRTLVGMKLLDKISSVPHGELSCTTELLILAHHCFTLTCHMEGIIRVLQAARLLTDKHLAPNEEYGLMVRLLTGIGRYNEMTYIFDLLHNKHYFEVLMRKKLDPSGSLKTALLDYIKRCRPGDSEKHNMIALCFSMCREIGENHEAAARIQLKLIESQPWEDSLKDGHHLKQLLLKALTLMLDAAESYAKDSCVRQAQHCHRLTKLITLQIHFLNTGQNTMLINLGRHRLMDCILALPRFYQASIVAEAYDFVPDWAEILYQQVILKGDFNYLEEFKQQRLLRSSIFEEISKKYKQHQPTDTAVKNLKKLLTYCEDVYLYYKLAYEHKFYDIVNVLLKDPQSGCCLKDMLAG

Target ORF information:

RefSeq Version	XM_003640025.4
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris SPG11, spatacsin vesicle trafficking associated (SPG11), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_003640025.4

ORF Insert Sequence:

ATGGCCGCAG GAGAGGCAGC TGCGAGAGCT GCTGGTTGCA GCAGCTGCGG CGCTGAAACT 
ATGGAACGGG TTCTACCTAT GCTGTTGGTG CCGGTCCCTG TGGAGGCAAC CGGGCGGTTG 
GGCTCCCGGG CGCAGTTGCA CAGAGAGCAG GAGGTTCTGG GGAGCCTAAC AGCTGCAGGC 
AGCCTGCAGG TGCTTTCCTT GGCGCCGGGC GGCCGGAGCG GCGGGGCCTG CTGCCTCAAT 
GGCCCCTTTC GGCACTTTAT ATGGGAGGAT TCTCATAACG GCAGCTCACC AACAGACAAG 
CCCAAACTTC TTGCTCTTAG TGAAAACTAT GAACTGTTTA TCTATGAATT TAATTTGAAA 
GATGGAAGAT GTGATGCAAC CATTTTGTAT AGCTGTAGTG AGGAGACATT GCAGAAGCTC 
ATTGAAGATC AAAATATCAG TGTTTCCTTT TTATCTTTGA GAATTCTGTC ATTTCACAAT 
AACACATCAT TATTGTTCAT CAATAAATGT ATTGTCCTAC ACATTATATT TCCTGAAAGA 
GATGCTGAGA TCAGAGTACT CAACTGTTTC ACGTTACCCT TGCCTGCACA GGCAGTGGAC 
AGGATCATTG ATGTGCAGCT CTGCAGAGGA ATTCTTTTTG TTTTGAGTAG TTTAGGCTGG 
ATCTACATTT TTGACATTGT GGATGGAAGA CATGTAGCTC ACGTGGATTT AGCACTTCAC 
CAAGAAGATA TGTGTAATGA ACAGCAACAG GAACTAGCCA AGATTTCTTC CTTTACTTCA 
CTGAAAGTGT CTCAAGACCT AGATGTTGTA GTGATTGTCA GCTCCTTCAA CTCTGCAGTT 
GCTCTTAACC TAAACTTGTA TTTCAGGCAA CACCCAGGAC ACCTACTGTG TGAAAGAACT 
CTAGAAGACA TTCCTATTGA AGGCCCTGAG GGGATCGATG AAGATGATCC TGTTAATTCT 
GACTACAACA TGAAACTGAC CAAGTTTTCC TTTCAAGTTG ATAGGTCTTG GAAAGCCCAG 
CTATCATCAC TGAATGAAAT AATAAAGAGC TCCAAATGGG AGGTTCCCTG TTGTGCTCCA 
TGGTTCCAGA ATATTTTGCA TTTGGAGTCA CCGGAATCTG GTTACCACAG TACTAGTACA 
CCAGGCTGGG CCTTCATCCC ACAGGATGAA ATTCATAGCC ACTACAAGTT TCCACAGAAA 
GATCATGCCA ACACCGGTGA CCCAGGAAGA TCATGGAAAA TAATGAACAT TAGTGAACTA 
AAGGAACCTG TTGAGCTTAC ATGTATGTCT GTGACAGGAT TCAGTGTACT GTTTACTTGG 
GCAGTGGAAA AGATGGGCTG CACCATTGTC CTTTGGGATT TGGAGACCCA GGGTATCCAG 
TGTTTTTCCC TTGGCAAAAG GTGTATTCCT ATAGATATGA GTGGAGACCA GCAGCTGTGC 
CTTGTTTTGA CAGAAAATGG ACTCTCCCTG ATTTTGTTTG GATTGACTCA GGAGGAGTTT 
TTAAATAGAC TAATGATCCA TGGAAGTGCC AGCACTGTGG ACTCTCTTTG CCATCTCAAT 
GGTTGGGGGA GGTGCTCAAT CCCCATACAT GCGCTGGAGG CTGGGATAGA AAATCGTCAG 
CTGGACACCG TAGATTTCTT TTTGAAGAGC AAAGAAAATC TTTTGAATCC ATCCTCAAAA 
TCTTCCGTAC CGGATCAGTT TGATGACTTT CCATCTCATT TATATTTAAG AAATGTGGAA 
AAGCTGACAC CAGCATTGGA TTTACTTTGC TCAGCAATTA GAGAAAGTGA TTCTGAAACC 
CAAAGCAAAC ACTTTTCAGA ACAGTTGCTT AACCTTACAC TGTCTTTTCT TAACAAACAA 
ATAAAGGAGC TTTTTGTTCA CAATGAAGAA TTAGATGAAC ATCTACAGAA AGGAGTGAAC 
ATTTTGACCA GCTACATTAA TGAACTTCGA ACCTTCATGA TAAAATTTCC TTGGAAGATA 
GTAGACCCAG TAGATGAATA TGATGTAAGT GAAAATGTCC TCAAAGTAAG AGAGAGTGAT 
ACATGGGAGA AACTCAGCAT TGAGGAAGTT ATTGCCAATG CTATTTTAAA CAACAAAATA 
CCAGAGGCAC AGACTTTCTT CAGGATTAAC AATCATTCTG CTCAAAGACT TGAGGAGCTG 
ATTAGAATAG GCCTAAATTT GGTCTTTGAC TGTTTGAAAA AGAACAATAT AAAGGAAGCA 
TCTGAACTTT TGAAGAATAT GGGCTTTGAT GTAAAAGACC AGTTGCTCAA GATATGTTTC 
TATACAACTG ATAAAAATAT ACGGGACTTT TTGGTGGAAA TTTTAAAAGA AAAAAATTAT 
TTTTCTGAAA ATGAAAAAAG AACTATAGAC TTCGTGCATC AAGTTGAGAA GTTTTATTCA 
GGACATTTTC AAGAAAATGT GCAGATCCAG TCCTTTACCA GGTATTGGAT AAAGGAACAG 
GATTTTTTCA AGCACAAATC TGTTTTGGAC TCACTCCTGA AATATGATCC TAAAGATAAA 
TTTAACAAAC AGGACCATAG AATTGTGTTA AATTGGGCTC AGTGGTGGAA TCAACTAACA 
CAAGAATCCA TCCTTCTCCC CAGGATAAGT CCAGAAGAAT ACAAATCATG TTCTCCTGAA 
GCCCTTTGGA GCTATCTCAC AGCCCATCAT GATTGGTCAA GCATTAGCTT GTGGATTGAA 
GAATTTCAGG CCCAGAACAG TTGTGTTTTA CTGCAGCAGA ACAAATGGCC CCTTCTGACT 
GTTGATGTTA TAGACCAGAA TACTTGCTGC AACAACTACA TGAGGAATGA AATTTTAGAT 
CAGCTGGCCA GGAATGGGAT TTTTCTTCAA TCTGAGCTAG AAGACTTTGA ACTCTTCCTG 
CTAAGACTGA ATCGCATTGG GGGTGTGATG CAAGATGCTC TCCCCATTCA AAACTACAGA 
TCCAAAGAAG GTTGGGATTT CCATTCTCAC TTCATTCTCT ATTGTTTGGA ACATGGTCTG 
CAGTATCCTC TCTATGTCTA TCTTGACTTT TACAAACTTA GTCCTGCAAA TTGTCCCTTT 
TTGGAAAAAA AAGAATTACA TGAAACTCAC CCTTGGTTTG AATTTTTGGT TCAGTGTCGA 
CAGGTTTCCA GTAATTTAAC AGATCCCAAA CTGATCTTCC AGGCTAGCCT TGCAAACGCT 
CAGATACTGA TTCCCAGCAA CCAGGCCAGT GTAAGCAGTA TGCTATTGGA AGGACATACT 
CTCCTGGCCC TTGCTACCAC AATGTATGCC CCTGGCGGTG TCAGCCAGGT TGTTCAGGAT 
GATGAAAATG AGACCTGTTT GAAGAAAGTA GATCCCCAGT TACTGAAGAT GGCATTAACT 
CCTTATCCCA AGCTAAAAAC TGCTCTCTTC CCACAGTACA CTACCTCTAA TGTTCTGCCG 
CCTGATATTA CGCTTTACCA CCTTATTCAG TCACTATTGC CCTTTGACTC TAGCAGATTG 
TTTGGCTGGC AGTCTGCCAA CACACTGGCT ATTGGAGATG CAGTGAGTCA TCTCCCACAT 
TTTTCTAGCC CTGATCTGGT TAATAAATAT GCTATCATGG AACGTCTGAA TTTTGCATAT 
TATTTACAAC ATGGACGGCC GTCATTTGCA TTTGGTACTT TTCTGGTCCA GGAATTAACC 
AAGAGCAAGA CTCCCAAGCA GCTGATCCAG CAAGTAGGCA ATGAAGCTTA TGTTTTAGGG 
CTCTCCTCCT TCTACATTCC ATCAGTAGGA GCTGCCTGTG TTTGTTTCTT AGAACTGCTT 
GGCCTCGATA GCCTCAAGCT CAGAGTTGAC ATGAAAGTGG CCAATATAAT TTTGAACTAC 
AAATGTAGAA ATGAAGATGT TCAGTACAGC TGTATCAGAG AGGCTCTAGT TGAAAAACTA 
TCTAAACTGG CTGTGGGTGA AAAGGCCACC GTAGAGGAAT TGCTTATTCT CTTAGAAGAA 
GGTATATGGA ACAACATTCA GCAGCAAGAG ATAAAGAGGT TATCTAGTGA ATCTAGCAGC 
CAGTGGGCAT TAGCAGTACA GTTCTGCAGG CTTCACAATG TGAAGCTGAG CACGTCTTAC 
CTTAGAGAAT GTGCCAAAGC AAATGACTGG TTACAGTTCA TTATTCACAG CCAACTCTAT 
AACTATCACC CAGCGGAGGT GAAATCTCTT CTTCAGTATT TCAGTCCTGT CCTTCAAGAC 
CACTTAAGAC TAGCTTTTGA GAACCTGACC TCAGTGTCTA AATCCAAAAT GGATAGTGGT 
GAAGTCTGCA GCAGGTCTCC CCAGGAACTC CAGAGAAACA AAGAAGAAAT GGCTGATTTC 
TTTCAAGTTC TACTCCAGTG CTCAGAGGAA CCAAACCCCT GGTGCTGGCT CCTAGCTGAA 
GCAATGAAAC AACAGGCTCC TATCCTTAGT GTCCTGGCCT CATGTCTTCA GGATGCCAGT 
ACTATTCCTT GTCTCTGTGT TTGGATCATC ACATCTGTGG AGGACAGTGT TGCAGCTGAA 
GCAACAGAAC ACATTCAGGG CTCAGTGGAA GGCCACATAT GGGACCTTCA AGACCTCTCA 
GTCATCTGGA GAACATTATT AACAAGGCAG AAGAACAAAA CTCTCATCAG AGGTTTCCAA 
CTTTTCTTTA AGGATTCCCC ACTACTGTTG ATGATGGAGA TGTATGAACT GTGTATGTTC 
TTCAAGAATT ATAAAGAAGC TGAAGCTAAA CTTCTGGAGT TCCAAAAGAG CTTTGAAACT 
GTTGAAACAG CAGCCACAAG GGTCCACACT ACCATCCCTG CCCCATGGCT GAGGGAGCAG 
GTGTGTTTTC TTCTGAAGCT TATGCTGCAG CAGTGTGGGA CCCAGTATGA ACTGGGGAAG 
CTTTTACAGC TATTTGTTGG AATAGAAAAT TTCTTCTCTG ATGGTCCAGA TGTGAAGAAG 
CTCTGTGTCC TTAGTGAAAT TTTGAAGGAC ACATCCATAG CCATTAATCA TGTAATTATT 
AGCAGCCACA GCCTTGAGAA TTTTCAGCAT GAATGTACAT CTATTTTGGA AAAACTGGCG 
ATGGAGGGAC AGTTTGCTTT GGCCAGGAGA GTAGCAGAAT TAGCTGAATT ACCTGTGGAC 
AACTTGGTTA TTGAAGAGAT GACACAGGAA ATGCAAACCT TAAAACACAT TGACCAGTGG 
TCCCTGAAAC AAGCAAGAAT TGACTTCTGG AAAAAATGTC ATGAGAATTT TAAGAAAAAT 
TCCATTTCAA GCAAAGCAGC TTCTTCCTTT TTCTCAGCCC AGGCACCTGA GTGCCCAACT 
GAAGAGGGTC GGAGCAGCAG TGAGGAGCGC CATCTGCTGC TCACATTGGC AGCACACTGG 
CTTGCTCAGG AGGATCTGGT GCCGGTGGCC GAACTGGAGG AGCTAGAGAA GCAGATCTGG 
CTCTGCCGCA TTACCCAATA CACCCTCAGA GAAAAACAGG AGCAAGTAGA GCTCGGGTTT 
TCTCAACAGA TTTCAACTAG TGGGGAACTT TCCTTTGATG GCATAGCCAG TAAGTTTTCA 
CTCTCCATGT TGGCTGTTCT GAACACATCA AAGTACTTAG AACTCAGTGG CTTTCCATCC 
AAAGAGACAT GTGATAGCGG ACTGGATTGG AAGGAGCAGG AGTCACTGAA CTTTCTGATT 
GGGCACCTAT TGGATGATGG CTTTGTGCAT GAAGCAAGTA GAGTGTGTCG GTATTTTCAT 
TTCTATAACC AAGATGTTGC CTTGGTGTTG CACTGCAGAG CACTGGCCTC AGGGGAAGCT 
AGTGTGGATG ACCTGCACCC AGAGATCCAT GCTCTCCTAC AAAGTGCTGA GCCGCTTGAG 
GAGGAGGAAG CTGGCATTCC ACTAAGAAAA GTCCAGAGCA GTTCAAGTTT GGATAGTCAG 
TCATTTGTGA TGCTGCCCCC CAGTGATGAA GTGGTGACTA ATCTGGAAAC TCTGACAAGT 
AAATGCCTCC ATGGGAAGAA CTACTGTCGG CAGGTCCTGT GTCTGTATGA ACTTGCCAAG 
GAGTTGGGCT GTTCCTACAC AGACGTGGCC TCTCAGGACG GGGAGGCCAT GCTCCGGGCA 
ATCTTGGCTT CCCAGCAGCC CGACCGATGC AAGCGAGCCC AGGCTTTCAT TAGCACCCAG 
GGCCTCGAGG CAGACACGGT GGCTGAACTT GTGGCTGAAG AGGTGACCCG GGAGCTGCTG 
ACCCCACCGG AGGGAACAGG ACATCAGCAG ATGTTTGCCC CAGCAGAGGA AAGCCGGACA 
TTTCTTCAGC TGACCACTCT GTGTCAAGAC CGCACACTGG TAGGCATGAA GTTGTTGGAC 
AAGATTTCGT CCGTTCCCCA TGGAGAATTA TCTTGCACCA CAGAGCTGCT GATCCTGGCC 
CATCACTGCT TCACACTGAC ATGCCACATG GAGGGCATCA TCCGAGTCCT GCAAGCTGCC 
CGGCTGCTCA CGGACAAACA CTTGGCCCCC AATGAAGAGT ACGGGCTGAT GGTACGCCTC 
CTCACCGGCA TCGGAAGGTA CAACGAGATG ACGTACATAT TTGACTTGCT GCATAATAAG 
CACTATTTTG AAGTGCTGAT GAGGAAGAAG TTGGATCCGA GTGGCTCCCT GAAGACCGCC 
CTACTGGACT ACATCAAACG CTGCCGCCCT GGGGACAGTG AGAAGCACAA CATGATCGCT 
CTGTGCTTCA GCATGTGTCG GGAGATTGGG GAGAACCATG AGGCAGCTGC CCGCATCCAG 
CTGAAACTGA TCGAGTCTCA GCCCTGGGAG GACAGCCTCA AGGACGGGCA CCACCTGAAG 
CAGCTGCTGC TGAAAGCCCT GACTCTGATG CTGGATGCAG CCGAGAGCTA TGCCAAGGAC 
TCCTGTGTGC GACAGGCCCA GCACTGTCAC CGGCTCACCA AGTTGATAAC ACTGCAGATT 
CATTTTCTGA ACACTGGCCA GAACACAATG CTCATCAACC TGGGCCGCCA CCGGCTGATG 
GACTGTATCC TCGCTCTACC TCGGTTCTAC CAGGCTTCTA TTGTGGCCGA AGCCTATGAC 
TTTGTTCCTG ATTGGGCAGA AATCCTCTAC CAACAAGTGA TTCTTAAAGG AGACTTTAAT 
TACCTGGAAG AATTTAAGCA GCAAAGGTTA TTAAGATCCA GTATATTTGA AGAGATTTCA 
AAAAAATATA AACAACATCA GCCGACAGAC ACGGCTGTGA AAAACCTGAA GAAGTTACTC 
ACATACTGTG AAGATGTCTA CTTGTACTAC AAGCTCGCAT ATGAACACAA ATTTTATGAC 
ATTGTGAATG TGCTTCTGAA GGACCCTCAG AGCGGCTGCT GTCTAAAGGA CATGCTAGCA 
GGTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SPG11 ( XM_005638363.2 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_005638420.1 Canis lupus familiaris spastic paraplegia 11 (autosomal recessive) (SPG11), transcript variant X4, mRNA.

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CloneID	OCe28793
Clone ID Related Accession (Same CDS sequence)	XM_005638363.2 , XM_005638363.3
Accession Version	XM_005638363.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6357bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1442332800000
Organism	Canis lupus familiaris(Dog)
Product	spatacsin isoform X4
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726113.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_005638363.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAACTGA CCAAGTTTTC CTTTCAAGTT GATAGGTCTT GGAAAGCCCA GCTATCATCA 
CTGAATGAAA TAATAAAGAG CTCCAAATGG GAGGTTCCCT GTTGTGCTCC ATGGTTCCAG 
AATATTTTGC ATTTGGAGTC ACCGGAATCT GGTTACCACA GTACTAGTAC ACCAGGCTGG 
GCCTTCATCC CACAGGATGA AATTCATAGC CACTACAAGT TTCCACAGAA AGATCATGCC 
AACACCGGTG ACCCAGGAAG ATCATGGAAA ATAATGAACA TTAGTGAACT AAAGGAACCT 
GTTGAGCTTA CATGTATGTC TGTGACAGGA TTCAGTGTAC TGTTTACTTG GGCAGTGGAA 
AAGATGGGCT GCACCATTGT CCTTTGGGAT TTGGAGACCC AGGGTATCCA GTGTTTTTCC 
CTTGGCAAAA GGTGTATTCC TATAGATATG AGTGGAGACC AGCAGCTGTG CCTTGTTTTG 
ACAGAAAATG GACTCTCCCT GATTTTGTTT GGATTGACTC AGGAGGAGTT TTTAAATAGA 
CTAATGATCC ATGGAAGTGC CAGCACTGTG GACTCTCTTT GCCATCTCAA TGGTTGGGGG 
AGGTGCTCAA TCCCCATACA TGCGCTGGAG GCTGGGATAG AAAATCGTCA GCTGGACACC 
GTAGATTTCT TTTTGAAGAG CAAAGAAAAT CTTTTGAATC CATCCTCAAA ATCTTCCGTA 
CCGGATCAGT TTGATGACTT TCCATCTCAT TTATATTTAA GAAATGTGGA AAAGCTGACA 
CCAGCATTGG ATTTACTTTG CTCAGCAATT AGAGAAAGTG ATTCTGAAAC CCAAAGCAAA 
CACTTTTCAG AACAGTTGCT TAACCTTACA CTGTCTTTTC TTAACAAACA AATAAAGGAG 
CTTTTTGTTC ACAATGAAGA ATTAGATGAA CATCTACAGA AAGGAGTGAA CATTTTGACC 
AGCTACATTA ATGAACTTCG AACCTTCATG ATAAAATTTC CTTGGAAGAT AGTAGACCCA 
GTAGATGAAT ATGATGTAAG TGAAAATGTC CTCAAAGTAA GAGAGAGTGA TACATGGGAG 
AAACTCAGCA TTGAGGAAGT TATTGCCAAT GCTATTTTAA ACAACAAAAT ACCAGAGGCA 
CAGACTTTCT TCAGGATTAA CAATCATTCT GCTCAAAGAC TTGAGGAGCT GATTAGAATA 
GGCCTAAATT TGGTCTTTGA CTGTTTGAAA AAGAACAATA TAAAGGAAGC ATCTGAACTT 
TTGAAGAATA TGGGCTTTGA TGTAAAAGAC CAGTTGCTCA AGATATGTTT CTATACAACT 
GATAAAAATA TACGGGACTT TTTGGTGGAA ATTTTAAAAG AAAAAAATTA TTTTTCTGAA 
AATGAAAAAA GAACTATAGA CTTCGTGCAT CAAGTTGAGA AGTTTTATTC AGGACATTTT 
CAAGAAAATG TGCAGATCCA GTCCTTTACC AGGTATTGGA TAAAGGAACA GGATTTTTTC 
AAGCACAAAT CTGTTTTGGA CTCACTCCTG AAATATGATC CTAAAGATAA ATTTAACAAA 
CAGGACCATA GAATTGTGTT AAATTGGGCT CAGTGGTGGA ATCAACTAAC ACAAGAATCC 
ATCCTTCTCC CCAGGATAAG TCCAGAAGAA TACAAATCAT GTTCTCCTGA AGCCCTTTGG 
AGCTATCTCA CAGCCCATCA TGATTGGTCA AGCATTAGCT TGTGGATTGA AGAATTTCAG 
GCCCAGAACA GTTGTGTTTT ACTGCAGCAG AACAAATGGC CCCTTCTGAC TGTTGATGTT 
ATAGACCAGA ATACTTGCTG CAACAACTAC ATGAGGAATG AAATTTTAGA TCAGCTGGCC 
AGGAATGGGA TTTTTCTTCA ATCTGAGCTA GAAGACTTTG AACTCTTCCT GCTAAGACTG 
AATCGCATTG GGGGTGTGAT GCAAGATGCT CTCCCCATTC AAAACTACAG ATCCAAAGAA 
GGTTGGGATT TCCATTCTCA CTTCATTCTC TATTGTTTGG AACATGGTCT GCAGTATCCT 
CTCTATGTCT ATCTTGACTT TTACAAACTT AGTCCTGCAA ATTGTCCCTT TTTGGAAAAA 
AAAGAATTAC ATGAAACTCA CCCTTGGTTT GAATTTTTGG TTCAGTGTCG ACAGGTTTCC 
AGTAATTTAA CAGATCCCAA ACTGATCTTC CAGGCTAGCC TTGCAAACGC TCAGATACTG 
ATTCCCAGCA ACCAGGCCAG TGTAAGCAGT ATGCTATTGG AAGGACATAC TCTCCTGGCC 
CTTGCTACCA CAATGTATGC CCCTGGCGGT GTCAGCCAGG TTGTTCAGGA TGATGAAAAT 
GAGACCTGTT TGAAGAAAGT AGATCCCCAG TTACTGAAGA TGGCATTAAC TCCTTATCCC 
AAGCTAAAAA CTGCTCTCTT CCCACAGTAC ACTACCTCTA ATGTTCTGCC GCCTGATATT 
ACGCTTTACC ACCTTATTCA GTCACTATTG CCCTTTGACT CTAGCAGATT GTTTGGCTGG 
CAGTCTGCCA ACACACTGGC TATTGGAGAT GCAGTGAGTC ATCTCCCACA TTTTTCTAGC 
CCTGATCTGG TTAATAAATA TGCTATCATG GAACGTCTGA ATTTTGCATA TTATTTACAA 
CATGGACGGC CGTCATTTGC ATTTGGTACT TTTCTGGTCC AGGAATTAAC CAAGAGCAAG 
ACTCCCAAGC AGCTGATCCA GCAAGTAGGC AATGAAGCTT ATGTTTTAGG GCTCTCCTCC 
TTCTACATTC CATCAGTAGG AGCTGCCTGT GTTTGTTTCT TAGAACTGCT TGGCCTCGAT 
AGCCTCAAGC TCAGAGTTGA CATGAAAGTG GCCAATATAA TTTTGAACTA CAAATGTAGA 
AATGAAGATG TTCAGTACAG CTGTATCAGA GAGGCTCTAG TTGAAAAACT ATCTAAACTG 
GCTGTGGGTG AAAAGGCCAC CGTAGAGGAA TTGCTTATTC TCTTAGAAGA AGGTATATGG 
AACAACATTC AGCAGCAAGA GATAAAGAGG TTATCTAGTG AATCTAGCAG CCAGTGGGCA 
TTAGCAGTAC AGTTCTGCAG GCTTCACAAT GTGAAGCTGA GCACGTCTTA CCTTAGAGAA 
TGTGCCAAAG CAAATGACTG GTTACAGTTC ATTATTCACA GCCAACTCTA TAACTATCAC 
CCAGCGGAGG TGAAATCTCT TCTTCAGTAT TTCAGTCCTG TCCTTCAAGA CCACTTAAGA 
CTAGCTTTTG AGAACCTGAC CTCAGTGTCT AAATCCAAAA TGGATAGTGG TGAAGTCTGC 
AGCAGGTCTC CCCAGGAACT CCAGAGAAAC AAAGAAGAAA TGGCTGATTT CTTTCAAGTT 
CTACTCCAGT GCTCAGAGGA ACCAAACCCC TGGTGCTGGC TCCTAGCTGA AGCAATGAAA 
CAACAGGCTC CTATCCTTAG TGTCCTGGCC TCATGTCTTC AGGATGCCAG TACTATTCCT 
TGTCTCTGTG TTTGGATCAT CACATCTGTG GAGGACAGTG TTGCAGCTGA AGCAACAGAA 
CACATTCAGG GCTCAGTGGA AGGCCACATA TGGGACCTTC AAGACCTCTC AGTCATCTGG 
AGAACATTAT TAACAAGGCA GAAGAACAAA ACTCTCATCA GAGGTTTCCA ACTTTTCTTT 
AAGGATTCCC CACTACTGTT GATGATGGAG ATGTATGAAC TGTGTATGTT CTTCAAGAAT 
TATAAAGAAG CTGAAGCTAA ACTTCTGGAG TTCCAAAAGA GCTTTGAAAC TGTTGAAACA 
GCAGCCACAA GGGTCCACAC TACCATCCCT GCCCCATGGC TGAGGGAGCA GGTGTGTTTT 
CTTCTGAAGC TTATGCTGCA GCAGTGTGGG ACCCAGTATG AACTGGGGAA GCTTTTACAG 
CTATTTGTTG GAATAGAAAA TTTCTTCTCT GATGGTCCAG ATGTGAAGAA GCTCTGTGTC 
CTTAGTGAAA TTTTGAAGGA CACATCCATA GCCATTAATC ATGTAATTAT TAGCAGCCAC 
AGCCTTGAGA ATTTTCAGCA TGAATGTACA TCTATTTTGG AAAAACTGGC GATGGAGGGA 
CAGTTTGCTT TGGCCAGGAG AGTAGCAGAA TTAGCTGAAT TACCTGTGGA CAACTTGGTT 
ATTGAAGAGA TGACACAGGA AATGCAAACC TTAAAACACA TTGACCAGTG GTCCCTGAAA 
CAAGCAAGAA TTGACTTCTG GAAAAAATGT CATGAGAATT TTAAGAAAAA TTCCATTTCA 
AGCAAAGCAG CTTCTTCCTT TTTCTCAGCC CAGGCACCTG AGTGCCCAAC TGAAGAGGGT 
CGGAGCAGCA GTGAGGAGCG CCATCTGCTG CTCACATTGG CAGCACACTG GCTTGCTCAG 
GAGGATCTGG TGCCGGTGGC CGAACTGGAG GAGCTAGAGA AGCAGATCTG GCTCTGCCGC 
ATTACCCAAT ACACCCTCAG AGAAAAACAG GAGCAAGTAG AGCTCGGGTT TTCTCAACAG 
ATTTCAACTA GTGGGGAACT TTCCTTTGAT GGCATAGCCA GTAAGTTTTC ACTCTCCATG 
TTGGCTGTTC TGAACACATC AAAGTACTTA GAACTCAGTG GCTTTCCATC CAAAGAGACA 
TGTGATAGCG GACTGGATTG GAAGGAGCAG GAGTCACTGA ACTTTCTGAT TGGGCACCTA 
TTGGATGATG GCTTTGTGCA TGAAGCAAGT AGAGTGTGTC GGTATTTTCA TTTCTATAAC 
CAAGATGTTG CCTTGGTGTT GCACTGCAGA GCACTGGCCT CAGGGGAAGC TAGTGTGGAT 
GACCTGCACC CAGAGATCCA TGCTCTCCTA CAAAGTGCTG AGCCGCTTGA GGAGGAGGAA 
GCTGGCATTC CACTAAGAAA AGTCCAGAGC AGTTCAAGTT TGGATAGTCA GTCATTTGTG 
ATGCTGCCCC CCAGTGATGA AGTGGTGACT AATCTGGAAA CTCTGACAAG TAAATGCCTC 
CATGGGAAGA ACTACTGTCG GCAGGTCCTG TGTCTGTATG AACTTGCCAA GGAGTTGGGC 
TGTTCCTACA CAGACGTGGC CTCTCAGGAC GGGGAGGCCA TGCTCCGGGC AATCTTGGCT 
TCCCAGCAGC CCGACCGATG CAAGCGAGCC CAGGCTTTCA TTAGCACCCA GGGCCTCGAG 
GCAGACACGG TGGCTGAACT TGTGGCTGAA GAGGTGACCC GGGAGCTGCT GACCCCACCG 
GAGGGAACAG GACATCAGCA GATGTTTGCC CCAGCAGAGG AAAGCCGGAC ATTTCTTCAG 
CTGACCACTC TGTGTCAAGA CCGCACACTG GTAGGCATGA AGTTGTTGGA CAAGATTTCG 
TCCGTTCCCC ATGGAGAATT ATCTTGCACC ACAGAGCTGC TGATCCTGGC CCATCACTGC 
TTCACACTGA CATGCCACAT GGAGGGCATC ATCCGAGTCC TGCAAGCTGC CCGGCTGCTC 
ACGGACAAAC ACTTGGCCCC CAATGAAGAG TACGGGCTGA TGGTACGCCT CCTCACCGGC 
ATCGGAAGGT ACAACGAGAT GACGTACATA TTTGACTTGC TGCATAATAA GCACTATTTT 
GAAGTGCTGA TGAGGAAGAA GTTGGATCCG AGTGGCTCCC TGAAGACCGC CCTACTGGAC 
TACATCAAAC GCTGCCGCCC TGGGGACAGT GAGAAGCACA ACATGATCGC TCTGTGCTTC 
AGCATGTGTC GGGAGATTGG GGAGAACCAT GAGGCAGCTG CCCGCATCCA GCTGAAACTG 
ATCGAGTCTC AGCCCTGGGA GGACAGCCTC AAGGACGGGC ACCACCTGAA GCAGCTGCTG 
CTGAAAGCCC TGACTCTGAT GCTGGATGCA GCCGAGAGCT ATGCCAAGGA CTCCTGTGTG 
CGACAGGCCC AGCACTGTCA CCGGCTCACC AAGTTGATAA CACTGCAGAT TCATTTTCTG 
AACACTGGCC AGAACACAAT GCTCATCAAC CTGGGCCGCC ACCGGCTGAT GGACTGTATC 
CTCGCTCTAC CTCGGTTCTA CCAGGCTTCT ATTGTGGCCG AAGCCTATGA CTTTGTTCCT 
GATTGGGCAG AAATCCTCTA CCAACAAGTG ATTCTTAAAG GAGACTTTAA TTACCTGGAA 
GAATTTAAGC AGCAAAGGTT ATTAAGATCC AGTATATTTG AAGAGATTTC AAAAAAATAT 
AAACAACATC AGCCGACAGA CACGGCTGTG AAAAACCTGA AGAAGTTACT CACATACTGT 
GAAGATGTCT ACTTGTACTA CAAGCTCGCA TATGAACACA AATTTTATGA CATTGTGAAT 
GTGCTTCTGA AGGACCCTCA GAGCGGCTGC TGTCTAAAGG ACATGCTAGC AGGTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005638420.1
CDS	187..6543
Translation	MKLTKFSFQVDRSWKAQLSSLNEIIKSSKWEVPCCAPWFQNILHLESPESGYHSTSTPGWAFIPQDEIHSHYKFPQKDHANTGDPGRSWKIMNISELKEPVELTCMSVTGFSVLFTWAVEKMGCTIVLWDLETQGIQCFSLGKRCIPIDMSGDQQLCLVLTENGLSLILFGLTQEEFLNRLMIHGSASTVDSLCHLNGWGRCSIPIHALEAGIENRQLDTVDFFLKSKENLLNPSSKSSVPDQFDDFPSHLYLRNVEKLTPALDLLCSAIRESDSETQSKHFSEQLLNLTLSFLNKQIKELFVHNEELDEHLQKGVNILTSYINELRTFMIKFPWKIVDPVDEYDVSENVLKVRESDTWEKLSIEEVIANAILNNKIPEAQTFFRINNHSAQRLEELIRIGLNLVFDCLKKNNIKEASELLKNMGFDVKDQLLKICFYTTDKNIRDFLVEILKEKNYFSENEKRTIDFVHQVEKFYSGHFQENVQIQSFTRYWIKEQDFFKHKSVLDSLLKYDPKDKFNKQDHRIVLNWAQWWNQLTQESILLPRISPEEYKSCSPEALWSYLTAHHDWSSISLWIEEFQAQNSCVLLQQNKWPLLTVDVIDQNTCCNNYMRNEILDQLARNGIFLQSELEDFELFLLRLNRIGGVMQDALPIQNYRSKEGWDFHSHFILYCLEHGLQYPLYVYLDFYKLSPANCPFLEKKELHETHPWFEFLVQCRQVSSNLTDPKLIFQASLANAQILIPSNQASVSSMLLEGHTLLALATTMYAPGGVSQVVQDDENETCLKKVDPQLLKMALTPYPKLKTALFPQYTTSNVLPPDITLYHLIQSLLPFDSSRLFGWQSANTLAIGDAVSHLPHFSSPDLVNKYAIMERLNFAYYLQHGRPSFAFGTFLVQELTKSKTPKQLIQQVGNEAYVLGLSSFYIPSVGAACVCFLELLGLDSLKLRVDMKVANIILNYKCRNEDVQYSCIREALVEKLSKLAVGEKATVEELLILLEEGIWNNIQQQEIKRLSSESSSQWALAVQFCRLHNVKLSTSYLRECAKANDWLQFIIHSQLYNYHPAEVKSLLQYFSPVLQDHLRLAFENLTSVSKSKMDSGEVCSRSPQELQRNKEEMADFFQVLLQCSEEPNPWCWLLAEAMKQQAPILSVLASCLQDASTIPCLCVWIITSVEDSVAAEATEHIQGSVEGHIWDLQDLSVIWRTLLTRQKNKTLIRGFQLFFKDSPLLLMMEMYELCMFFKNYKEAEAKLLEFQKSFETVETAATRVHTTIPAPWLREQVCFLLKLMLQQCGTQYELGKLLQLFVGIENFFSDGPDVKKLCVLSEILKDTSIAINHVIISSHSLENFQHECTSILEKLAMEGQFALARRVAELAELPVDNLVIEEMTQEMQTLKHIDQWSLKQARIDFWKKCHENFKKNSISSKAASSFFSAQAPECPTEEGRSSSEERHLLLTLAAHWLAQEDLVPVAELEELEKQIWLCRITQYTLREKQEQVELGFSQQISTSGELSFDGIASKFSLSMLAVLNTSKYLELSGFPSKETCDSGLDWKEQESLNFLIGHLLDDGFVHEASRVCRYFHFYNQDVALVLHCRALASGEASVDDLHPEIHALLQSAEPLEEEEAGIPLRKVQSSSSLDSQSFVMLPPSDEVVTNLETLTSKCLHGKNYCRQVLCLYELAKELGCSYTDVASQDGEAMLRAILASQQPDRCKRAQAFISTQGLEADTVAELVAEEVTRELLTPPEGTGHQQMFAPAEESRTFLQLTTLCQDRTLVGMKLLDKISSVPHGELSCTTELLILAHHCFTLTCHMEGIIRVLQAARLLTDKHLAPNEEYGLMVRLLTGIGRYNEMTYIFDLLHNKHYFEVLMRKKLDPSGSLKTALLDYIKRCRPGDSEKHNMIALCFSMCREIGENHEAAARIQLKLIESQPWEDSLKDGHHLKQLLLKALTLMLDAAESYAKDSCVRQAQHCHRLTKLITLQIHFLNTGQNTMLINLGRHRLMDCILALPRFYQASIVAEAYDFVPDWAEILYQQVILKGDFNYLEEFKQQRLLRSSIFEEISKKYKQHQPTDTAVKNLKKLLTYCEDVYLYYKLAYEHKFYDIVNVLLKDPQSGCCLKDMLAG

Target ORF information:

RefSeq Version	XM_005638363.2
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris spastic paraplegia 11 (autosomal recessive) (SPG11), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005638363.2

ORF Insert Sequence:

ATGAAACTGA CCAAGTTTTC CTTTCAAGTT GATAGGTCTT GGAAAGCCCA GCTATCATCA 
CTGAATGAAA TAATAAAGAG CTCCAAATGG GAGGTTCCCT GTTGTGCTCC ATGGTTCCAG 
AATATTTTGC ATTTGGAGTC ACCGGAATCT GGTTACCACA GTACTAGTAC ACCAGGCTGG 
GCCTTCATCC CACAGGATGA AATTCATAGC CACTACAAGT TTCCACAGAA AGATCATGCC 
AACACCGGTG ACCCAGGAAG ATCATGGAAA ATAATGAACA TTAGTGAACT AAAGGAACCT 
GTTGAGCTTA CATGTATGTC TGTGACAGGA TTCAGTGTAC TGTTTACTTG GGCAGTGGAA 
AAGATGGGCT GCACCATTGT CCTTTGGGAT TTGGAGACCC AGGGTATCCA GTGTTTTTCC 
CTTGGCAAAA GGTGTATTCC TATAGATATG AGTGGAGACC AGCAGCTGTG CCTTGTTTTG 
ACAGAAAATG GACTCTCCCT GATTTTGTTT GGATTGACTC AGGAGGAGTT TTTAAATAGA 
CTAATGATCC ATGGAAGTGC CAGCACTGTG GACTCTCTTT GCCATCTCAA TGGTTGGGGG 
AGGTGCTCAA TCCCCATACA TGCGCTGGAG GCTGGGATAG AAAATCGTCA GCTGGACACC 
GTAGATTTCT TTTTGAAGAG CAAAGAAAAT CTTTTGAATC CATCCTCAAA ATCTTCCGTA 
CCGGATCAGT TTGATGACTT TCCATCTCAT TTATATTTAA GAAATGTGGA AAAGCTGACA 
CCAGCATTGG ATTTACTTTG CTCAGCAATT AGAGAAAGTG ATTCTGAAAC CCAAAGCAAA 
CACTTTTCAG AACAGTTGCT TAACCTTACA CTGTCTTTTC TTAACAAACA AATAAAGGAG 
CTTTTTGTTC ACAATGAAGA ATTAGATGAA CATCTACAGA AAGGAGTGAA CATTTTGACC 
AGCTACATTA ATGAACTTCG AACCTTCATG ATAAAATTTC CTTGGAAGAT AGTAGACCCA 
GTAGATGAAT ATGATGTAAG TGAAAATGTC CTCAAAGTAA GAGAGAGTGA TACATGGGAG 
AAACTCAGCA TTGAGGAAGT TATTGCCAAT GCTATTTTAA ACAACAAAAT ACCAGAGGCA 
CAGACTTTCT TCAGGATTAA CAATCATTCT GCTCAAAGAC TTGAGGAGCT GATTAGAATA 
GGCCTAAATT TGGTCTTTGA CTGTTTGAAA AAGAACAATA TAAAGGAAGC ATCTGAACTT 
TTGAAGAATA TGGGCTTTGA TGTAAAAGAC CAGTTGCTCA AGATATGTTT CTATACAACT 
GATAAAAATA TACGGGACTT TTTGGTGGAA ATTTTAAAAG AAAAAAATTA TTTTTCTGAA 
AATGAAAAAA GAACTATAGA CTTCGTGCAT CAAGTTGAGA AGTTTTATTC AGGACATTTT 
CAAGAAAATG TGCAGATCCA GTCCTTTACC AGGTATTGGA TAAAGGAACA GGATTTTTTC 
AAGCACAAAT CTGTTTTGGA CTCACTCCTG AAATATGATC CTAAAGATAA ATTTAACAAA 
CAGGACCATA GAATTGTGTT AAATTGGGCT CAGTGGTGGA ATCAACTAAC ACAAGAATCC 
ATCCTTCTCC CCAGGATAAG TCCAGAAGAA TACAAATCAT GTTCTCCTGA AGCCCTTTGG 
AGCTATCTCA CAGCCCATCA TGATTGGTCA AGCATTAGCT TGTGGATTGA AGAATTTCAG 
GCCCAGAACA GTTGTGTTTT ACTGCAGCAG AACAAATGGC CCCTTCTGAC TGTTGATGTT 
ATAGACCAGA ATACTTGCTG CAACAACTAC ATGAGGAATG AAATTTTAGA TCAGCTGGCC 
AGGAATGGGA TTTTTCTTCA ATCTGAGCTA GAAGACTTTG AACTCTTCCT GCTAAGACTG 
AATCGCATTG GGGGTGTGAT GCAAGATGCT CTCCCCATTC AAAACTACAG ATCCAAAGAA 
GGTTGGGATT TCCATTCTCA CTTCATTCTC TATTGTTTGG AACATGGTCT GCAGTATCCT 
CTCTATGTCT ATCTTGACTT TTACAAACTT AGTCCTGCAA ATTGTCCCTT TTTGGAAAAA 
AAAGAATTAC ATGAAACTCA CCCTTGGTTT GAATTTTTGG TTCAGTGTCG ACAGGTTTCC 
AGTAATTTAA CAGATCCCAA ACTGATCTTC CAGGCTAGCC TTGCAAACGC TCAGATACTG 
ATTCCCAGCA ACCAGGCCAG TGTAAGCAGT ATGCTATTGG AAGGACATAC TCTCCTGGCC 
CTTGCTACCA CAATGTATGC CCCTGGCGGT GTCAGCCAGG TTGTTCAGGA TGATGAAAAT 
GAGACCTGTT TGAAGAAAGT AGATCCCCAG TTACTGAAGA TGGCATTAAC TCCTTATCCC 
AAGCTAAAAA CTGCTCTCTT CCCACAGTAC ACTACCTCTA ATGTTCTGCC GCCTGATATT 
ACGCTTTACC ACCTTATTCA GTCACTATTG CCCTTTGACT CTAGCAGATT GTTTGGCTGG 
CAGTCTGCCA ACACACTGGC TATTGGAGAT GCAGTGAGTC ATCTCCCACA TTTTTCTAGC 
CCTGATCTGG TTAATAAATA TGCTATCATG GAACGTCTGA ATTTTGCATA TTATTTACAA 
CATGGACGGC CGTCATTTGC ATTTGGTACT TTTCTGGTCC AGGAATTAAC CAAGAGCAAG 
ACTCCCAAGC AGCTGATCCA GCAAGTAGGC AATGAAGCTT ATGTTTTAGG GCTCTCCTCC 
TTCTACATTC CATCAGTAGG AGCTGCCTGT GTTTGTTTCT TAGAACTGCT TGGCCTCGAT 
AGCCTCAAGC TCAGAGTTGA CATGAAAGTG GCCAATATAA TTTTGAACTA CAAATGTAGA 
AATGAAGATG TTCAGTACAG CTGTATCAGA GAGGCTCTAG TTGAAAAACT ATCTAAACTG 
GCTGTGGGTG AAAAGGCCAC CGTAGAGGAA TTGCTTATTC TCTTAGAAGA AGGTATATGG 
AACAACATTC AGCAGCAAGA GATAAAGAGG TTATCTAGTG AATCTAGCAG CCAGTGGGCA 
TTAGCAGTAC AGTTCTGCAG GCTTCACAAT GTGAAGCTGA GCACGTCTTA CCTTAGAGAA 
TGTGCCAAAG CAAATGACTG GTTACAGTTC ATTATTCACA GCCAACTCTA TAACTATCAC 
CCAGCGGAGG TGAAATCTCT TCTTCAGTAT TTCAGTCCTG TCCTTCAAGA CCACTTAAGA 
CTAGCTTTTG AGAACCTGAC CTCAGTGTCT AAATCCAAAA TGGATAGTGG TGAAGTCTGC 
AGCAGGTCTC CCCAGGAACT CCAGAGAAAC AAAGAAGAAA TGGCTGATTT CTTTCAAGTT 
CTACTCCAGT GCTCAGAGGA ACCAAACCCC TGGTGCTGGC TCCTAGCTGA AGCAATGAAA 
CAACAGGCTC CTATCCTTAG TGTCCTGGCC TCATGTCTTC AGGATGCCAG TACTATTCCT 
TGTCTCTGTG TTTGGATCAT CACATCTGTG GAGGACAGTG TTGCAGCTGA AGCAACAGAA 
CACATTCAGG GCTCAGTGGA AGGCCACATA TGGGACCTTC AAGACCTCTC AGTCATCTGG 
AGAACATTAT TAACAAGGCA GAAGAACAAA ACTCTCATCA GAGGTTTCCA ACTTTTCTTT 
AAGGATTCCC CACTACTGTT GATGATGGAG ATGTATGAAC TGTGTATGTT CTTCAAGAAT 
TATAAAGAAG CTGAAGCTAA ACTTCTGGAG TTCCAAAAGA GCTTTGAAAC TGTTGAAACA 
GCAGCCACAA GGGTCCACAC TACCATCCCT GCCCCATGGC TGAGGGAGCA GGTGTGTTTT 
CTTCTGAAGC TTATGCTGCA GCAGTGTGGG ACCCAGTATG AACTGGGGAA GCTTTTACAG 
CTATTTGTTG GAATAGAAAA TTTCTTCTCT GATGGTCCAG ATGTGAAGAA GCTCTGTGTC 
CTTAGTGAAA TTTTGAAGGA CACATCCATA GCCATTAATC ATGTAATTAT TAGCAGCCAC 
AGCCTTGAGA ATTTTCAGCA TGAATGTACA TCTATTTTGG AAAAACTGGC GATGGAGGGA 
CAGTTTGCTT TGGCCAGGAG AGTAGCAGAA TTAGCTGAAT TACCTGTGGA CAACTTGGTT 
ATTGAAGAGA TGACACAGGA AATGCAAACC TTAAAACACA TTGACCAGTG GTCCCTGAAA 
CAAGCAAGAA TTGACTTCTG GAAAAAATGT CATGAGAATT TTAAGAAAAA TTCCATTTCA 
AGCAAAGCAG CTTCTTCCTT TTTCTCAGCC CAGGCACCTG AGTGCCCAAC TGAAGAGGGT 
CGGAGCAGCA GTGAGGAGCG CCATCTGCTG CTCACATTGG CAGCACACTG GCTTGCTCAG 
GAGGATCTGG TGCCGGTGGC CGAACTGGAG GAGCTAGAGA AGCAGATCTG GCTCTGCCGC 
ATTACCCAAT ACACCCTCAG AGAAAAACAG GAGCAAGTAG AGCTCGGGTT TTCTCAACAG 
ATTTCAACTA GTGGGGAACT TTCCTTTGAT GGCATAGCCA GTAAGTTTTC ACTCTCCATG 
TTGGCTGTTC TGAACACATC AAAGTACTTA GAACTCAGTG GCTTTCCATC CAAAGAGACA 
TGTGATAGCG GACTGGATTG GAAGGAGCAG GAGTCACTGA ACTTTCTGAT TGGGCACCTA 
TTGGATGATG GCTTTGTGCA TGAAGCAAGT AGAGTGTGTC GGTATTTTCA TTTCTATAAC 
CAAGATGTTG CCTTGGTGTT GCACTGCAGA GCACTGGCCT CAGGGGAAGC TAGTGTGGAT 
GACCTGCACC CAGAGATCCA TGCTCTCCTA CAAAGTGCTG AGCCGCTTGA GGAGGAGGAA 
GCTGGCATTC CACTAAGAAA AGTCCAGAGC AGTTCAAGTT TGGATAGTCA GTCATTTGTG 
ATGCTGCCCC CCAGTGATGA AGTGGTGACT AATCTGGAAA CTCTGACAAG TAAATGCCTC 
CATGGGAAGA ACTACTGTCG GCAGGTCCTG TGTCTGTATG AACTTGCCAA GGAGTTGGGC 
TGTTCCTACA CAGACGTGGC CTCTCAGGAC GGGGAGGCCA TGCTCCGGGC AATCTTGGCT 
TCCCAGCAGC CCGACCGATG CAAGCGAGCC CAGGCTTTCA TTAGCACCCA GGGCCTCGAG 
GCAGACACGG TGGCTGAACT TGTGGCTGAA GAGGTGACCC GGGAGCTGCT GACCCCACCG 
GAGGGAACAG GACATCAGCA GATGTTTGCC CCAGCAGAGG AAAGCCGGAC ATTTCTTCAG 
CTGACCACTC TGTGTCAAGA CCGCACACTG GTAGGCATGA AGTTGTTGGA CAAGATTTCG 
TCCGTTCCCC ATGGAGAATT ATCTTGCACC ACAGAGCTGC TGATCCTGGC CCATCACTGC 
TTCACACTGA CATGCCACAT GGAGGGCATC ATCCGAGTCC TGCAAGCTGC CCGGCTGCTC 
ACGGACAAAC ACTTGGCCCC CAATGAAGAG TACGGGCTGA TGGTACGCCT CCTCACCGGC 
ATCGGAAGGT ACAACGAGAT GACGTACATA TTTGACTTGC TGCATAATAA GCACTATTTT 
GAAGTGCTGA TGAGGAAGAA GTTGGATCCG AGTGGCTCCC TGAAGACCGC CCTACTGGAC 
TACATCAAAC GCTGCCGCCC TGGGGACAGT GAGAAGCACA ACATGATCGC TCTGTGCTTC 
AGCATGTGTC GGGAGATTGG GGAGAACCAT GAGGCAGCTG CCCGCATCCA GCTGAAACTG 
ATCGAGTCTC AGCCCTGGGA GGACAGCCTC AAGGACGGGC ACCACCTGAA GCAGCTGCTG 
CTGAAAGCCC TGACTCTGAT GCTGGATGCA GCCGAGAGCT ATGCCAAGGA CTCCTGTGTG 
CGACAGGCCC AGCACTGTCA CCGGCTCACC AAGTTGATAA CACTGCAGAT TCATTTTCTG 
AACACTGGCC AGAACACAAT GCTCATCAAC CTGGGCCGCC ACCGGCTGAT GGACTGTATC 
CTCGCTCTAC CTCGGTTCTA CCAGGCTTCT ATTGTGGCCG AAGCCTATGA CTTTGTTCCT 
GATTGGGCAG AAATCCTCTA CCAACAAGTG ATTCTTAAAG GAGACTTTAA TTACCTGGAA 
GAATTTAAGC AGCAAAGGTT ATTAAGATCC AGTATATTTG AAGAGATTTC AAAAAAATAT 
AAACAACATC AGCCGACAGA CACGGCTGTG AAAAACCTGA AGAAGTTACT CACATACTGT 
GAAGATGTCT ACTTGTACTA CAAGCTCGCA TATGAACACA AATTTTATGA CATTGTGAAT 
GTGCTTCTGA AGGACCCTCA GAGCGGCTGC TGTCTAAAGG ACATGCTAGC AGGTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SPG11 ( XM_005638363.3 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_005638420.1 Canis lupus familiaris SPG11, spatacsin vesicle trafficking associated (SPG11), transcript variant X4, mRNA.

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CloneID	OCe28793
Clone ID Related Accession (Same CDS sequence)	XM_005638363.2 , XM_005638363.3
Accession Version	XM_005638363.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6357bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1504454400000
Organism	Canis lupus familiaris(dog)
Product	spatacsin isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006612.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_005638363.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAACTGA CCAAGTTTTC CTTTCAAGTT GATAGGTCTT GGAAAGCCCA GCTATCATCA 
CTGAATGAAA TAATAAAGAG CTCCAAATGG GAGGTTCCCT GTTGTGCTCC ATGGTTCCAG 
AATATTTTGC ATTTGGAGTC ACCGGAATCT GGTTACCACA GTACTAGTAC ACCAGGCTGG 
GCCTTCATCC CACAGGATGA AATTCATAGC CACTACAAGT TTCCACAGAA AGATCATGCC 
AACACCGGTG ACCCAGGAAG ATCATGGAAA ATAATGAACA TTAGTGAACT AAAGGAACCT 
GTTGAGCTTA CATGTATGTC TGTGACAGGA TTCAGTGTAC TGTTTACTTG GGCAGTGGAA 
AAGATGGGCT GCACCATTGT CCTTTGGGAT TTGGAGACCC AGGGTATCCA GTGTTTTTCC 
CTTGGCAAAA GGTGTATTCC TATAGATATG AGTGGAGACC AGCAGCTGTG CCTTGTTTTG 
ACAGAAAATG GACTCTCCCT GATTTTGTTT GGATTGACTC AGGAGGAGTT TTTAAATAGA 
CTAATGATCC ATGGAAGTGC CAGCACTGTG GACTCTCTTT GCCATCTCAA TGGTTGGGGG 
AGGTGCTCAA TCCCCATACA TGCGCTGGAG GCTGGGATAG AAAATCGTCA GCTGGACACC 
GTAGATTTCT TTTTGAAGAG CAAAGAAAAT CTTTTGAATC CATCCTCAAA ATCTTCCGTA 
CCGGATCAGT TTGATGACTT TCCATCTCAT TTATATTTAA GAAATGTGGA AAAGCTGACA 
CCAGCATTGG ATTTACTTTG CTCAGCAATT AGAGAAAGTG ATTCTGAAAC CCAAAGCAAA 
CACTTTTCAG AACAGTTGCT TAACCTTACA CTGTCTTTTC TTAACAAACA AATAAAGGAG 
CTTTTTGTTC ACAATGAAGA ATTAGATGAA CATCTACAGA AAGGAGTGAA CATTTTGACC 
AGCTACATTA ATGAACTTCG AACCTTCATG ATAAAATTTC CTTGGAAGAT AGTAGACCCA 
GTAGATGAAT ATGATGTAAG TGAAAATGTC CTCAAAGTAA GAGAGAGTGA TACATGGGAG 
AAACTCAGCA TTGAGGAAGT TATTGCCAAT GCTATTTTAA ACAACAAAAT ACCAGAGGCA 
CAGACTTTCT TCAGGATTAA CAATCATTCT GCTCAAAGAC TTGAGGAGCT GATTAGAATA 
GGCCTAAATT TGGTCTTTGA CTGTTTGAAA AAGAACAATA TAAAGGAAGC ATCTGAACTT 
TTGAAGAATA TGGGCTTTGA TGTAAAAGAC CAGTTGCTCA AGATATGTTT CTATACAACT 
GATAAAAATA TACGGGACTT TTTGGTGGAA ATTTTAAAAG AAAAAAATTA TTTTTCTGAA 
AATGAAAAAA GAACTATAGA CTTCGTGCAT CAAGTTGAGA AGTTTTATTC AGGACATTTT 
CAAGAAAATG TGCAGATCCA GTCCTTTACC AGGTATTGGA TAAAGGAACA GGATTTTTTC 
AAGCACAAAT CTGTTTTGGA CTCACTCCTG AAATATGATC CTAAAGATAA ATTTAACAAA 
CAGGACCATA GAATTGTGTT AAATTGGGCT CAGTGGTGGA ATCAACTAAC ACAAGAATCC 
ATCCTTCTCC CCAGGATAAG TCCAGAAGAA TACAAATCAT GTTCTCCTGA AGCCCTTTGG 
AGCTATCTCA CAGCCCATCA TGATTGGTCA AGCATTAGCT TGTGGATTGA AGAATTTCAG 
GCCCAGAACA GTTGTGTTTT ACTGCAGCAG AACAAATGGC CCCTTCTGAC TGTTGATGTT 
ATAGACCAGA ATACTTGCTG CAACAACTAC ATGAGGAATG AAATTTTAGA TCAGCTGGCC 
AGGAATGGGA TTTTTCTTCA ATCTGAGCTA GAAGACTTTG AACTCTTCCT GCTAAGACTG 
AATCGCATTG GGGGTGTGAT GCAAGATGCT CTCCCCATTC AAAACTACAG ATCCAAAGAA 
GGTTGGGATT TCCATTCTCA CTTCATTCTC TATTGTTTGG AACATGGTCT GCAGTATCCT 
CTCTATGTCT ATCTTGACTT TTACAAACTT AGTCCTGCAA ATTGTCCCTT TTTGGAAAAA 
AAAGAATTAC ATGAAACTCA CCCTTGGTTT GAATTTTTGG TTCAGTGTCG ACAGGTTTCC 
AGTAATTTAA CAGATCCCAA ACTGATCTTC CAGGCTAGCC TTGCAAACGC TCAGATACTG 
ATTCCCAGCA ACCAGGCCAG TGTAAGCAGT ATGCTATTGG AAGGACATAC TCTCCTGGCC 
CTTGCTACCA CAATGTATGC CCCTGGCGGT GTCAGCCAGG TTGTTCAGGA TGATGAAAAT 
GAGACCTGTT TGAAGAAAGT AGATCCCCAG TTACTGAAGA TGGCATTAAC TCCTTATCCC 
AAGCTAAAAA CTGCTCTCTT CCCACAGTAC ACTACCTCTA ATGTTCTGCC GCCTGATATT 
ACGCTTTACC ACCTTATTCA GTCACTATTG CCCTTTGACT CTAGCAGATT GTTTGGCTGG 
CAGTCTGCCA ACACACTGGC TATTGGAGAT GCAGTGAGTC ATCTCCCACA TTTTTCTAGC 
CCTGATCTGG TTAATAAATA TGCTATCATG GAACGTCTGA ATTTTGCATA TTATTTACAA 
CATGGACGGC CGTCATTTGC ATTTGGTACT TTTCTGGTCC AGGAATTAAC CAAGAGCAAG 
ACTCCCAAGC AGCTGATCCA GCAAGTAGGC AATGAAGCTT ATGTTTTAGG GCTCTCCTCC 
TTCTACATTC CATCAGTAGG AGCTGCCTGT GTTTGTTTCT TAGAACTGCT TGGCCTCGAT 
AGCCTCAAGC TCAGAGTTGA CATGAAAGTG GCCAATATAA TTTTGAACTA CAAATGTAGA 
AATGAAGATG TTCAGTACAG CTGTATCAGA GAGGCTCTAG TTGAAAAACT ATCTAAACTG 
GCTGTGGGTG AAAAGGCCAC CGTAGAGGAA TTGCTTATTC TCTTAGAAGA AGGTATATGG 
AACAACATTC AGCAGCAAGA GATAAAGAGG TTATCTAGTG AATCTAGCAG CCAGTGGGCA 
TTAGCAGTAC AGTTCTGCAG GCTTCACAAT GTGAAGCTGA GCACGTCTTA CCTTAGAGAA 
TGTGCCAAAG CAAATGACTG GTTACAGTTC ATTATTCACA GCCAACTCTA TAACTATCAC 
CCAGCGGAGG TGAAATCTCT TCTTCAGTAT TTCAGTCCTG TCCTTCAAGA CCACTTAAGA 
CTAGCTTTTG AGAACCTGAC CTCAGTGTCT AAATCCAAAA TGGATAGTGG TGAAGTCTGC 
AGCAGGTCTC CCCAGGAACT CCAGAGAAAC AAAGAAGAAA TGGCTGATTT CTTTCAAGTT 
CTACTCCAGT GCTCAGAGGA ACCAAACCCC TGGTGCTGGC TCCTAGCTGA AGCAATGAAA 
CAACAGGCTC CTATCCTTAG TGTCCTGGCC TCATGTCTTC AGGATGCCAG TACTATTCCT 
TGTCTCTGTG TTTGGATCAT CACATCTGTG GAGGACAGTG TTGCAGCTGA AGCAACAGAA 
CACATTCAGG GCTCAGTGGA AGGCCACATA TGGGACCTTC AAGACCTCTC AGTCATCTGG 
AGAACATTAT TAACAAGGCA GAAGAACAAA ACTCTCATCA GAGGTTTCCA ACTTTTCTTT 
AAGGATTCCC CACTACTGTT GATGATGGAG ATGTATGAAC TGTGTATGTT CTTCAAGAAT 
TATAAAGAAG CTGAAGCTAA ACTTCTGGAG TTCCAAAAGA GCTTTGAAAC TGTTGAAACA 
GCAGCCACAA GGGTCCACAC TACCATCCCT GCCCCATGGC TGAGGGAGCA GGTGTGTTTT 
CTTCTGAAGC TTATGCTGCA GCAGTGTGGG ACCCAGTATG AACTGGGGAA GCTTTTACAG 
CTATTTGTTG GAATAGAAAA TTTCTTCTCT GATGGTCCAG ATGTGAAGAA GCTCTGTGTC 
CTTAGTGAAA TTTTGAAGGA CACATCCATA GCCATTAATC ATGTAATTAT TAGCAGCCAC 
AGCCTTGAGA ATTTTCAGCA TGAATGTACA TCTATTTTGG AAAAACTGGC GATGGAGGGA 
CAGTTTGCTT TGGCCAGGAG AGTAGCAGAA TTAGCTGAAT TACCTGTGGA CAACTTGGTT 
ATTGAAGAGA TGACACAGGA AATGCAAACC TTAAAACACA TTGACCAGTG GTCCCTGAAA 
CAAGCAAGAA TTGACTTCTG GAAAAAATGT CATGAGAATT TTAAGAAAAA TTCCATTTCA 
AGCAAAGCAG CTTCTTCCTT TTTCTCAGCC CAGGCACCTG AGTGCCCAAC TGAAGAGGGT 
CGGAGCAGCA GTGAGGAGCG CCATCTGCTG CTCACATTGG CAGCACACTG GCTTGCTCAG 
GAGGATCTGG TGCCGGTGGC CGAACTGGAG GAGCTAGAGA AGCAGATCTG GCTCTGCCGC 
ATTACCCAAT ACACCCTCAG AGAAAAACAG GAGCAAGTAG AGCTCGGGTT TTCTCAACAG 
ATTTCAACTA GTGGGGAACT TTCCTTTGAT GGCATAGCCA GTAAGTTTTC ACTCTCCATG 
TTGGCTGTTC TGAACACATC AAAGTACTTA GAACTCAGTG GCTTTCCATC CAAAGAGACA 
TGTGATAGCG GACTGGATTG GAAGGAGCAG GAGTCACTGA ACTTTCTGAT TGGGCACCTA 
TTGGATGATG GCTTTGTGCA TGAAGCAAGT AGAGTGTGTC GGTATTTTCA TTTCTATAAC 
CAAGATGTTG CCTTGGTGTT GCACTGCAGA GCACTGGCCT CAGGGGAAGC TAGTGTGGAT 
GACCTGCACC CAGAGATCCA TGCTCTCCTA CAAAGTGCTG AGCCGCTTGA GGAGGAGGAA 
GCTGGCATTC CACTAAGAAA AGTCCAGAGC AGTTCAAGTT TGGATAGTCA GTCATTTGTG 
ATGCTGCCCC CCAGTGATGA AGTGGTGACT AATCTGGAAA CTCTGACAAG TAAATGCCTC 
CATGGGAAGA ACTACTGTCG GCAGGTCCTG TGTCTGTATG AACTTGCCAA GGAGTTGGGC 
TGTTCCTACA CAGACGTGGC CTCTCAGGAC GGGGAGGCCA TGCTCCGGGC AATCTTGGCT 
TCCCAGCAGC CCGACCGATG CAAGCGAGCC CAGGCTTTCA TTAGCACCCA GGGCCTCGAG 
GCAGACACGG TGGCTGAACT TGTGGCTGAA GAGGTGACCC GGGAGCTGCT GACCCCACCG 
GAGGGAACAG GACATCAGCA GATGTTTGCC CCAGCAGAGG AAAGCCGGAC ATTTCTTCAG 
CTGACCACTC TGTGTCAAGA CCGCACACTG GTAGGCATGA AGTTGTTGGA CAAGATTTCG 
TCCGTTCCCC ATGGAGAATT ATCTTGCACC ACAGAGCTGC TGATCCTGGC CCATCACTGC 
TTCACACTGA CATGCCACAT GGAGGGCATC ATCCGAGTCC TGCAAGCTGC CCGGCTGCTC 
ACGGACAAAC ACTTGGCCCC CAATGAAGAG TACGGGCTGA TGGTACGCCT CCTCACCGGC 
ATCGGAAGGT ACAACGAGAT GACGTACATA TTTGACTTGC TGCATAATAA GCACTATTTT 
GAAGTGCTGA TGAGGAAGAA GTTGGATCCG AGTGGCTCCC TGAAGACCGC CCTACTGGAC 
TACATCAAAC GCTGCCGCCC TGGGGACAGT GAGAAGCACA ACATGATCGC TCTGTGCTTC 
AGCATGTGTC GGGAGATTGG GGAGAACCAT GAGGCAGCTG CCCGCATCCA GCTGAAACTG 
ATCGAGTCTC AGCCCTGGGA GGACAGCCTC AAGGACGGGC ACCACCTGAA GCAGCTGCTG 
CTGAAAGCCC TGACTCTGAT GCTGGATGCA GCCGAGAGCT ATGCCAAGGA CTCCTGTGTG 
CGACAGGCCC AGCACTGTCA CCGGCTCACC AAGTTGATAA CACTGCAGAT TCATTTTCTG 
AACACTGGCC AGAACACAAT GCTCATCAAC CTGGGCCGCC ACCGGCTGAT GGACTGTATC 
CTCGCTCTAC CTCGGTTCTA CCAGGCTTCT ATTGTGGCCG AAGCCTATGA CTTTGTTCCT 
GATTGGGCAG AAATCCTCTA CCAACAAGTG ATTCTTAAAG GAGACTTTAA TTACCTGGAA 
GAATTTAAGC AGCAAAGGTT ATTAAGATCC AGTATATTTG AAGAGATTTC AAAAAAATAT 
AAACAACATC AGCCGACAGA CACGGCTGTG AAAAACCTGA AGAAGTTACT CACATACTGT 
GAAGATGTCT ACTTGTACTA CAAGCTCGCA TATGAACACA AATTTTATGA CATTGTGAAT 
GTGCTTCTGA AGGACCCTCA GAGCGGCTGC TGTCTAAAGG ACATGCTAGC AGGTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005638420.1
CDS	238..6594
Translation	MKLTKFSFQVDRSWKAQLSSLNEIIKSSKWEVPCCAPWFQNILHLESPESGYHSTSTPGWAFIPQDEIHSHYKFPQKDHANTGDPGRSWKIMNISELKEPVELTCMSVTGFSVLFTWAVEKMGCTIVLWDLETQGIQCFSLGKRCIPIDMSGDQQLCLVLTENGLSLILFGLTQEEFLNRLMIHGSASTVDSLCHLNGWGRCSIPIHALEAGIENRQLDTVDFFLKSKENLLNPSSKSSVPDQFDDFPSHLYLRNVEKLTPALDLLCSAIRESDSETQSKHFSEQLLNLTLSFLNKQIKELFVHNEELDEHLQKGVNILTSYINELRTFMIKFPWKIVDPVDEYDVSENVLKVRESDTWEKLSIEEVIANAILNNKIPEAQTFFRINNHSAQRLEELIRIGLNLVFDCLKKNNIKEASELLKNMGFDVKDQLLKICFYTTDKNIRDFLVEILKEKNYFSENEKRTIDFVHQVEKFYSGHFQENVQIQSFTRYWIKEQDFFKHKSVLDSLLKYDPKDKFNKQDHRIVLNWAQWWNQLTQESILLPRISPEEYKSCSPEALWSYLTAHHDWSSISLWIEEFQAQNSCVLLQQNKWPLLTVDVIDQNTCCNNYMRNEILDQLARNGIFLQSELEDFELFLLRLNRIGGVMQDALPIQNYRSKEGWDFHSHFILYCLEHGLQYPLYVYLDFYKLSPANCPFLEKKELHETHPWFEFLVQCRQVSSNLTDPKLIFQASLANAQILIPSNQASVSSMLLEGHTLLALATTMYAPGGVSQVVQDDENETCLKKVDPQLLKMALTPYPKLKTALFPQYTTSNVLPPDITLYHLIQSLLPFDSSRLFGWQSANTLAIGDAVSHLPHFSSPDLVNKYAIMERLNFAYYLQHGRPSFAFGTFLVQELTKSKTPKQLIQQVGNEAYVLGLSSFYIPSVGAACVCFLELLGLDSLKLRVDMKVANIILNYKCRNEDVQYSCIREALVEKLSKLAVGEKATVEELLILLEEGIWNNIQQQEIKRLSSESSSQWALAVQFCRLHNVKLSTSYLRECAKANDWLQFIIHSQLYNYHPAEVKSLLQYFSPVLQDHLRLAFENLTSVSKSKMDSGEVCSRSPQELQRNKEEMADFFQVLLQCSEEPNPWCWLLAEAMKQQAPILSVLASCLQDASTIPCLCVWIITSVEDSVAAEATEHIQGSVEGHIWDLQDLSVIWRTLLTRQKNKTLIRGFQLFFKDSPLLLMMEMYELCMFFKNYKEAEAKLLEFQKSFETVETAATRVHTTIPAPWLREQVCFLLKLMLQQCGTQYELGKLLQLFVGIENFFSDGPDVKKLCVLSEILKDTSIAINHVIISSHSLENFQHECTSILEKLAMEGQFALARRVAELAELPVDNLVIEEMTQEMQTLKHIDQWSLKQARIDFWKKCHENFKKNSISSKAASSFFSAQAPECPTEEGRSSSEERHLLLTLAAHWLAQEDLVPVAELEELEKQIWLCRITQYTLREKQEQVELGFSQQISTSGELSFDGIASKFSLSMLAVLNTSKYLELSGFPSKETCDSGLDWKEQESLNFLIGHLLDDGFVHEASRVCRYFHFYNQDVALVLHCRALASGEASVDDLHPEIHALLQSAEPLEEEEAGIPLRKVQSSSSLDSQSFVMLPPSDEVVTNLETLTSKCLHGKNYCRQVLCLYELAKELGCSYTDVASQDGEAMLRAILASQQPDRCKRAQAFISTQGLEADTVAELVAEEVTRELLTPPEGTGHQQMFAPAEESRTFLQLTTLCQDRTLVGMKLLDKISSVPHGELSCTTELLILAHHCFTLTCHMEGIIRVLQAARLLTDKHLAPNEEYGLMVRLLTGIGRYNEMTYIFDLLHNKHYFEVLMRKKLDPSGSLKTALLDYIKRCRPGDSEKHNMIALCFSMCREIGENHEAAARIQLKLIESQPWEDSLKDGHHLKQLLLKALTLMLDAAESYAKDSCVRQAQHCHRLTKLITLQIHFLNTGQNTMLINLGRHRLMDCILALPRFYQASIVAEAYDFVPDWAEILYQQVILKGDFNYLEEFKQQRLLRSSIFEEISKKYKQHQPTDTAVKNLKKLLTYCEDVYLYYKLAYEHKFYDIVNVLLKDPQSGCCLKDMLAG

Target ORF information:

RefSeq Version	XM_005638363.3
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris SPG11, spatacsin vesicle trafficking associated (SPG11), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005638363.3

ORF Insert Sequence:

ATGAAACTGA CCAAGTTTTC CTTTCAAGTT GATAGGTCTT GGAAAGCCCA GCTATCATCA 
CTGAATGAAA TAATAAAGAG CTCCAAATGG GAGGTTCCCT GTTGTGCTCC ATGGTTCCAG 
AATATTTTGC ATTTGGAGTC ACCGGAATCT GGTTACCACA GTACTAGTAC ACCAGGCTGG 
GCCTTCATCC CACAGGATGA AATTCATAGC CACTACAAGT TTCCACAGAA AGATCATGCC 
AACACCGGTG ACCCAGGAAG ATCATGGAAA ATAATGAACA TTAGTGAACT AAAGGAACCT 
GTTGAGCTTA CATGTATGTC TGTGACAGGA TTCAGTGTAC TGTTTACTTG GGCAGTGGAA 
AAGATGGGCT GCACCATTGT CCTTTGGGAT TTGGAGACCC AGGGTATCCA GTGTTTTTCC 
CTTGGCAAAA GGTGTATTCC TATAGATATG AGTGGAGACC AGCAGCTGTG CCTTGTTTTG 
ACAGAAAATG GACTCTCCCT GATTTTGTTT GGATTGACTC AGGAGGAGTT TTTAAATAGA 
CTAATGATCC ATGGAAGTGC CAGCACTGTG GACTCTCTTT GCCATCTCAA TGGTTGGGGG 
AGGTGCTCAA TCCCCATACA TGCGCTGGAG GCTGGGATAG AAAATCGTCA GCTGGACACC 
GTAGATTTCT TTTTGAAGAG CAAAGAAAAT CTTTTGAATC CATCCTCAAA ATCTTCCGTA 
CCGGATCAGT TTGATGACTT TCCATCTCAT TTATATTTAA GAAATGTGGA AAAGCTGACA 
CCAGCATTGG ATTTACTTTG CTCAGCAATT AGAGAAAGTG ATTCTGAAAC CCAAAGCAAA 
CACTTTTCAG AACAGTTGCT TAACCTTACA CTGTCTTTTC TTAACAAACA AATAAAGGAG 
CTTTTTGTTC ACAATGAAGA ATTAGATGAA CATCTACAGA AAGGAGTGAA CATTTTGACC 
AGCTACATTA ATGAACTTCG AACCTTCATG ATAAAATTTC CTTGGAAGAT AGTAGACCCA 
GTAGATGAAT ATGATGTAAG TGAAAATGTC CTCAAAGTAA GAGAGAGTGA TACATGGGAG 
AAACTCAGCA TTGAGGAAGT TATTGCCAAT GCTATTTTAA ACAACAAAAT ACCAGAGGCA 
CAGACTTTCT TCAGGATTAA CAATCATTCT GCTCAAAGAC TTGAGGAGCT GATTAGAATA 
GGCCTAAATT TGGTCTTTGA CTGTTTGAAA AAGAACAATA TAAAGGAAGC ATCTGAACTT 
TTGAAGAATA TGGGCTTTGA TGTAAAAGAC CAGTTGCTCA AGATATGTTT CTATACAACT 
GATAAAAATA TACGGGACTT TTTGGTGGAA ATTTTAAAAG AAAAAAATTA TTTTTCTGAA 
AATGAAAAAA GAACTATAGA CTTCGTGCAT CAAGTTGAGA AGTTTTATTC AGGACATTTT 
CAAGAAAATG TGCAGATCCA GTCCTTTACC AGGTATTGGA TAAAGGAACA GGATTTTTTC 
AAGCACAAAT CTGTTTTGGA CTCACTCCTG AAATATGATC CTAAAGATAA ATTTAACAAA 
CAGGACCATA GAATTGTGTT AAATTGGGCT CAGTGGTGGA ATCAACTAAC ACAAGAATCC 
ATCCTTCTCC CCAGGATAAG TCCAGAAGAA TACAAATCAT GTTCTCCTGA AGCCCTTTGG 
AGCTATCTCA CAGCCCATCA TGATTGGTCA AGCATTAGCT TGTGGATTGA AGAATTTCAG 
GCCCAGAACA GTTGTGTTTT ACTGCAGCAG AACAAATGGC CCCTTCTGAC TGTTGATGTT 
ATAGACCAGA ATACTTGCTG CAACAACTAC ATGAGGAATG AAATTTTAGA TCAGCTGGCC 
AGGAATGGGA TTTTTCTTCA ATCTGAGCTA GAAGACTTTG AACTCTTCCT GCTAAGACTG 
AATCGCATTG GGGGTGTGAT GCAAGATGCT CTCCCCATTC AAAACTACAG ATCCAAAGAA 
GGTTGGGATT TCCATTCTCA CTTCATTCTC TATTGTTTGG AACATGGTCT GCAGTATCCT 
CTCTATGTCT ATCTTGACTT TTACAAACTT AGTCCTGCAA ATTGTCCCTT TTTGGAAAAA 
AAAGAATTAC ATGAAACTCA CCCTTGGTTT GAATTTTTGG TTCAGTGTCG ACAGGTTTCC 
AGTAATTTAA CAGATCCCAA ACTGATCTTC CAGGCTAGCC TTGCAAACGC TCAGATACTG 
ATTCCCAGCA ACCAGGCCAG TGTAAGCAGT ATGCTATTGG AAGGACATAC TCTCCTGGCC 
CTTGCTACCA CAATGTATGC CCCTGGCGGT GTCAGCCAGG TTGTTCAGGA TGATGAAAAT 
GAGACCTGTT TGAAGAAAGT AGATCCCCAG TTACTGAAGA TGGCATTAAC TCCTTATCCC 
AAGCTAAAAA CTGCTCTCTT CCCACAGTAC ACTACCTCTA ATGTTCTGCC GCCTGATATT 
ACGCTTTACC ACCTTATTCA GTCACTATTG CCCTTTGACT CTAGCAGATT GTTTGGCTGG 
CAGTCTGCCA ACACACTGGC TATTGGAGAT GCAGTGAGTC ATCTCCCACA TTTTTCTAGC 
CCTGATCTGG TTAATAAATA TGCTATCATG GAACGTCTGA ATTTTGCATA TTATTTACAA 
CATGGACGGC CGTCATTTGC ATTTGGTACT TTTCTGGTCC AGGAATTAAC CAAGAGCAAG 
ACTCCCAAGC AGCTGATCCA GCAAGTAGGC AATGAAGCTT ATGTTTTAGG GCTCTCCTCC 
TTCTACATTC CATCAGTAGG AGCTGCCTGT GTTTGTTTCT TAGAACTGCT TGGCCTCGAT 
AGCCTCAAGC TCAGAGTTGA CATGAAAGTG GCCAATATAA TTTTGAACTA CAAATGTAGA 
AATGAAGATG TTCAGTACAG CTGTATCAGA GAGGCTCTAG TTGAAAAACT ATCTAAACTG 
GCTGTGGGTG AAAAGGCCAC CGTAGAGGAA TTGCTTATTC TCTTAGAAGA AGGTATATGG 
AACAACATTC AGCAGCAAGA GATAAAGAGG TTATCTAGTG AATCTAGCAG CCAGTGGGCA 
TTAGCAGTAC AGTTCTGCAG GCTTCACAAT GTGAAGCTGA GCACGTCTTA CCTTAGAGAA 
TGTGCCAAAG CAAATGACTG GTTACAGTTC ATTATTCACA GCCAACTCTA TAACTATCAC 
CCAGCGGAGG TGAAATCTCT TCTTCAGTAT TTCAGTCCTG TCCTTCAAGA CCACTTAAGA 
CTAGCTTTTG AGAACCTGAC CTCAGTGTCT AAATCCAAAA TGGATAGTGG TGAAGTCTGC 
AGCAGGTCTC CCCAGGAACT CCAGAGAAAC AAAGAAGAAA TGGCTGATTT CTTTCAAGTT 
CTACTCCAGT GCTCAGAGGA ACCAAACCCC TGGTGCTGGC TCCTAGCTGA AGCAATGAAA 
CAACAGGCTC CTATCCTTAG TGTCCTGGCC TCATGTCTTC AGGATGCCAG TACTATTCCT 
TGTCTCTGTG TTTGGATCAT CACATCTGTG GAGGACAGTG TTGCAGCTGA AGCAACAGAA 
CACATTCAGG GCTCAGTGGA AGGCCACATA TGGGACCTTC AAGACCTCTC AGTCATCTGG 
AGAACATTAT TAACAAGGCA GAAGAACAAA ACTCTCATCA GAGGTTTCCA ACTTTTCTTT 
AAGGATTCCC CACTACTGTT GATGATGGAG ATGTATGAAC TGTGTATGTT CTTCAAGAAT 
TATAAAGAAG CTGAAGCTAA ACTTCTGGAG TTCCAAAAGA GCTTTGAAAC TGTTGAAACA 
GCAGCCACAA GGGTCCACAC TACCATCCCT GCCCCATGGC TGAGGGAGCA GGTGTGTTTT 
CTTCTGAAGC TTATGCTGCA GCAGTGTGGG ACCCAGTATG AACTGGGGAA GCTTTTACAG 
CTATTTGTTG GAATAGAAAA TTTCTTCTCT GATGGTCCAG ATGTGAAGAA GCTCTGTGTC 
CTTAGTGAAA TTTTGAAGGA CACATCCATA GCCATTAATC ATGTAATTAT TAGCAGCCAC 
AGCCTTGAGA ATTTTCAGCA TGAATGTACA TCTATTTTGG AAAAACTGGC GATGGAGGGA 
CAGTTTGCTT TGGCCAGGAG AGTAGCAGAA TTAGCTGAAT TACCTGTGGA CAACTTGGTT 
ATTGAAGAGA TGACACAGGA AATGCAAACC TTAAAACACA TTGACCAGTG GTCCCTGAAA 
CAAGCAAGAA TTGACTTCTG GAAAAAATGT CATGAGAATT TTAAGAAAAA TTCCATTTCA 
AGCAAAGCAG CTTCTTCCTT TTTCTCAGCC CAGGCACCTG AGTGCCCAAC TGAAGAGGGT 
CGGAGCAGCA GTGAGGAGCG CCATCTGCTG CTCACATTGG CAGCACACTG GCTTGCTCAG 
GAGGATCTGG TGCCGGTGGC CGAACTGGAG GAGCTAGAGA AGCAGATCTG GCTCTGCCGC 
ATTACCCAAT ACACCCTCAG AGAAAAACAG GAGCAAGTAG AGCTCGGGTT TTCTCAACAG 
ATTTCAACTA GTGGGGAACT TTCCTTTGAT GGCATAGCCA GTAAGTTTTC ACTCTCCATG 
TTGGCTGTTC TGAACACATC AAAGTACTTA GAACTCAGTG GCTTTCCATC CAAAGAGACA 
TGTGATAGCG GACTGGATTG GAAGGAGCAG GAGTCACTGA ACTTTCTGAT TGGGCACCTA 
TTGGATGATG GCTTTGTGCA TGAAGCAAGT AGAGTGTGTC GGTATTTTCA TTTCTATAAC 
CAAGATGTTG CCTTGGTGTT GCACTGCAGA GCACTGGCCT CAGGGGAAGC TAGTGTGGAT 
GACCTGCACC CAGAGATCCA TGCTCTCCTA CAAAGTGCTG AGCCGCTTGA GGAGGAGGAA 
GCTGGCATTC CACTAAGAAA AGTCCAGAGC AGTTCAAGTT TGGATAGTCA GTCATTTGTG 
ATGCTGCCCC CCAGTGATGA AGTGGTGACT AATCTGGAAA CTCTGACAAG TAAATGCCTC 
CATGGGAAGA ACTACTGTCG GCAGGTCCTG TGTCTGTATG AACTTGCCAA GGAGTTGGGC 
TGTTCCTACA CAGACGTGGC CTCTCAGGAC GGGGAGGCCA TGCTCCGGGC AATCTTGGCT 
TCCCAGCAGC CCGACCGATG CAAGCGAGCC CAGGCTTTCA TTAGCACCCA GGGCCTCGAG 
GCAGACACGG TGGCTGAACT TGTGGCTGAA GAGGTGACCC GGGAGCTGCT GACCCCACCG 
GAGGGAACAG GACATCAGCA GATGTTTGCC CCAGCAGAGG AAAGCCGGAC ATTTCTTCAG 
CTGACCACTC TGTGTCAAGA CCGCACACTG GTAGGCATGA AGTTGTTGGA CAAGATTTCG 
TCCGTTCCCC ATGGAGAATT ATCTTGCACC ACAGAGCTGC TGATCCTGGC CCATCACTGC 
TTCACACTGA CATGCCACAT GGAGGGCATC ATCCGAGTCC TGCAAGCTGC CCGGCTGCTC 
ACGGACAAAC ACTTGGCCCC CAATGAAGAG TACGGGCTGA TGGTACGCCT CCTCACCGGC 
ATCGGAAGGT ACAACGAGAT GACGTACATA TTTGACTTGC TGCATAATAA GCACTATTTT 
GAAGTGCTGA TGAGGAAGAA GTTGGATCCG AGTGGCTCCC TGAAGACCGC CCTACTGGAC 
TACATCAAAC GCTGCCGCCC TGGGGACAGT GAGAAGCACA ACATGATCGC TCTGTGCTTC 
AGCATGTGTC GGGAGATTGG GGAGAACCAT GAGGCAGCTG CCCGCATCCA GCTGAAACTG 
ATCGAGTCTC AGCCCTGGGA GGACAGCCTC AAGGACGGGC ACCACCTGAA GCAGCTGCTG 
CTGAAAGCCC TGACTCTGAT GCTGGATGCA GCCGAGAGCT ATGCCAAGGA CTCCTGTGTG 
CGACAGGCCC AGCACTGTCA CCGGCTCACC AAGTTGATAA CACTGCAGAT TCATTTTCTG 
AACACTGGCC AGAACACAAT GCTCATCAAC CTGGGCCGCC ACCGGCTGAT GGACTGTATC 
CTCGCTCTAC CTCGGTTCTA CCAGGCTTCT ATTGTGGCCG AAGCCTATGA CTTTGTTCCT 
GATTGGGCAG AAATCCTCTA CCAACAAGTG ATTCTTAAAG GAGACTTTAA TTACCTGGAA 
GAATTTAAGC AGCAAAGGTT ATTAAGATCC AGTATATTTG AAGAGATTTC AAAAAAATAT 
AAACAACATC AGCCGACAGA CACGGCTGTG AAAAACCTGA AGAAGTTACT CACATACTGT 
GAAGATGTCT ACTTGTACTA CAAGCTCGCA TATGAACACA AATTTTATGA CATTGTGAAT 
GTGCTTCTGA AGGACCCTCA GAGCGGCTGC TGTCTAAAGG ACATGCTAGC AGGTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SPG11 ( XM_014109490.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_013964965.1 Canis lupus familiaris spastic paraplegia 11 (autosomal recessive) (SPG11), transcript variant X2, mRNA.

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CloneID	OCe44278
Clone ID Related Accession (Same CDS sequence)	XM_014109490.1 , XM_014109490.2
Accession Version	XM_014109490.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7122bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1442332800000
Organism	Canis lupus familiaris(Dog)
Product	spatacsin isoform X2
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726113.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCGCAG GAGAGGCAGC TGCGAGAGCT GCTGGTTGCA GCAGCTGCGG CGCTGAAACT 
ATGGAACGGG TTCTACCTAT GCTGTTGGTG CCGGTCCCTG TGGAGGCAAC CGGGCGGTTG 
GGCTCCCGGG CGCAGTTGCA CAGAGAGCAG GAGGTTCTGG GGAGCCTAAC AGCTGCAGGC 
AGCCTGCAGG TGCTTTCCTT GGCGCCGGGC GGCCGGAGCG GCGGGGCCTG CTGCCTCAAT 
GGCCCCTTTC GGCACTTTAT ATGGGAGGAT TCTCATAACG GCAGCTCACC AACAGACAAG 
CCCAAACTTC TTGCTCTTAG TGAAAACTAT GAACTGTTTA TCTATGAATT TAATTTGAAA 
GATGGAAGAT GTGATGCAAC CATTTTGTAT AGCTGTAGTG AGGAGACATT GCAGAAGCTC 
ATTGAAGATC AAAATATCAG TGTTTCCTTT TTATCTTTGA GAATTCTGTC ATTTCACAAT 
AACACATCAT TATTGTTCAT CAATAAATGT ATTGTCCTAC ACATTATATT TCCTGAAAGA 
GATGCTGAGA TCAGAGTACT CAACTGTTTC ACGTTACCCT TGCCTGCACA GGCAGTGGAC 
AGGATCATTG ATGTGCAGCT CTGCAGAGGA ATTCTTTTTG TTTTGAGTAG TTTAGGCTGG 
ATCTACATTT TTGACATTGT GGATGGAAGA CATGTAGCTC ACGTGGATTT AGCACTTCAC 
CAAGAAGATA TGTGTAATGA ACAGCAACAG GAACTAGCCA AGATTTCTTC CTTTACTTCA 
CTGAAAGTGT CTCAAGACCT AGATGTTGTA GTGATTGTCA GCTCCTTCAA CTCTGCAGTT 
GCTCTTAACC TAAACTTGTA TTTCAGGCAA CACCCAGGAC ACCTACTGTG TGAAAGAACT 
CTAGAAGACA TTCCTATTGA AGGCCCTGAG GGGATCGATG AAGATGATCC TGTTAATTCT 
GACTACAACA TGAAACTGAC CAAGTTTTCC TTTCAAGTTG ATAGGTCTTG GAAAGCCCAG 
CTATCATCAC TGAATGAAAT AATAAAGAGC TCCAAATGGG AGGTTCCCTG TTGTGCTCCA 
TGGTTCCAGA ATATTTTGCA TTTGGAGTCA CCGGAATCTG GTTACCACAG TACTAGTACA 
CCAGGCTGGG CCTTCATCCC ACAGGATGAA ATTCATAGCC ACTACAAGTT TCCACAGAAA 
GATCATGCCA ACACCGGTGA CCCAGGAAGA TCATGGAAAA TAATGAACAT TAGTGAACTA 
AAGGAACCTG TTGAGCTTAC ATGTATGTCT GTGACAGGAT TCAGTGTACT GTTTACTTGG 
GCAGTGGAAA AGATGGGCTG CACCATTGTC CTTTGGGATT TGGAGACCCA GGGTATCCAG 
TGTTTTTCCC TTGGCAAAAG GTGTATTCCT ATAGATATGA GTGGAGACCA GCAGCTGTGC 
CTTGTTTTGA CAGAAAATGG ACTCTCCCTG ATTTTGTTTG GATTGACTCA GGAGGAGTTT 
TTAAATAGAC TAATGATCCA TGGAAGTGCC AGCACTGTGG ACTCTCTTTG CCATCTCAAT 
GGTTGGGGGA GGTGCTCAAT CCCCATACAT GCGCTGGAGG CTGGGATAGA AAATCGTCAG 
CTGGACACCG TAGATTTCTT TTTGAAGAGC AAAGAAAATC TTTTGAATCC ATCCTCAAAA 
TCTTCCGTAC CGGATCAGTT TGATGACTTT CCATCTCATT TATATTTAAG AAATGTGGAA 
AAGCTGACAC CAGCATTGGA TTTACTTTGC TCAGCAATTA GAGAAAGTGA TTCTGAAACC 
CAAAGCAAAC ACTTTTCAGA ACAGTTGCTT AACCTTACAC TGTCTTTTCT TAACAAACAA 
ATAAAGGAGC TTTTTGTTCA CAATGAAGAA TTAGATGAAC ATCTACAGAA AGGAGTGAAC 
ATTTTGACCA GCTACATTAA TGAACTTCGA ACCTTCATGA TAAAATTTCC TTGGAAGATA 
GTAGACCCAG TAGATGAATA TGATGTAAGT GAAAATGTCC TCAAAGTAAG AGAGAGTGAT 
ACATGGGAGA AACTCAGCAT TGAGGAAGTT ATTGCCAATG CTATTTTAAA CAACAAAATA 
CCAGAGGCAC AGACTTTCTT CAGGATTAAC AATCATTCTG CTCAAAGACT TGAGGAGCTG 
ATTAGAATAG GCCTAAATTT GGTCTTTGAC TGTTTGAAAA AGAACAATAT AAAGGAAGCA 
TCTGAACTTT TGAAGAATAT GGGCTTTGAT GTAAAAGACC AGTTGCTCAA GATATGTTTC 
TATACAACTG ATAAAAATAT ACGGGACTTT TTGGTGGAAA TTTTAAAAGA AAAAAATTAT 
TTTTCTGAAA ATGAAAAAAG AACTATAGAC TTCGTGCATC AAGTTGAGAA GTTTTATTCA 
GGACATTTTC AAGAAAATGT GCAGATCCAG TCCTTTACCA GGTATTGGAT AAAGGAACAG 
GATTTTTTCA AGCACAAATC TGTTTTGGAC TCACTCCTGA AATATGATCC TAAAGATAAA 
TTTAACAAAC AGGACCATAG AATTGTGTTA AATTGGGCTC AGTGGTGGAA TCAACTAACA 
CAAGAATCCA TCCTTCTCCC CAGGATAAGT CCAGAAGAAT ACAAATCATG TTCTCCTGAA 
GCCCTTTGGA GCTATCTCAC AGCCCATCAT GATTGGTCAA GCATTAGCTT GTGGATTGAA 
GAATTTCAGG CCCAGAACAG TTGTGTTTTA CTGCAGCAGA ACAAATGGCC CCTTCTGACT 
GTTGATGTTA TAGACCAGAA TACTTGCTGC AACAACTACA TGAGGAATGA AATTTTAGAT 
CAGCTGGCCA GACTTAGTCC TGCAAATTGT CCCTTTTTGG AAAAAAAAGA ATTACATGAA 
ACTCACCCTT GGTTTGAATT TTTGGTTCAG TGTCGACAGG TTTCCAGTAA TTTAACAGAT 
CCCAAACTGA TCTTCCAGGC TAGCCTTGCA AACGCTCAGA TACTGATTCC CAGCAACCAG 
GCCAGTGTAA GCAGTATGCT ATTGGAAGGA CATACTCTCC TGGCCCTTGC TACCACAATG 
TATGCCCCTG GCGGTGTCAG CCAGGTTGTT CAGGATGATG AAAATGAGAC CTGTTTGAAG 
AAAGTAGATC CCCAGTTACT GAAGATGGCA TTAACTCCTT ATCCCAAGCT AAAAACTGCT 
CTCTTCCCAC AGTACACTAC CTCTAATGTT CTGCCGCCTG ATATTACGCT TTACCACCTT 
ATTCAGTCAC TATTGCCCTT TGACTCTAGC AGATTGTTTG GCTGGCAGTC TGCCAACACA 
CTGGCTATTG GAGATGCAGT GAGTCATCTC CCACATTTTT CTAGCCCTGA TCTGGTTAAT 
AAATATGCTA TCATGGAACG TCTGAATTTT GCATATTATT TACAACATGG ACGGCCGTCA 
TTTGCATTTG GTACTTTTCT GGTCCAGGAA TTAACCAAGA GCAAGACTCC CAAGCAGCTG 
ATCCAGCAAG TAGGCAATGA AGCTTATGTT TTAGGGCTCT CCTCCTTCTA CATTCCATCA 
GTAGGAGCTG CCTGTGTTTG TTTCTTAGAA CTGCTTGGCC TCGATAGCCT CAAGCTCAGA 
GTTGACATGA AAGTGGCCAA TATAATTTTG AACTACAAAT GTAGAAATGA AGATGTTCAG 
TACAGCTGTA TCAGAGAGGC TCTAGTTGAA AAACTATCTA AACTGGCTGT GGGTGAAAAG 
GCCACCGTAG AGGAATTGCT TATTCTCTTA GAAGAAGGTA TATGGAACAA CATTCAGCAG 
CAAGAGATAA AGAGGTTATC TAGTGAATCT AGCAGCCAGT GGGCATTAGC AGTACAGTTC 
TGCAGGCTTC ACAATGTGAA GCTGAGCACG TCTTACCTTA GAGAATGTGC CAAAGCAAAT 
GACTGGTTAC AGTTCATTAT TCACAGCCAA CTCTATAACT ATCACCCAGC GGAGGTGAAA 
TCTCTTCTTC AGTATTTCAG TCCTGTCCTT CAAGACCACT TAAGACTAGC TTTTGAGAAC 
CTGACCTCAG TGTCTAAATC CAAAATGGAT AGTGGTGAAG TCTGCAGCAG GTCTCCCCAG 
GAACTCCAGA GAAACAAAGA AGAAATGGCT GATTTCTTTC AAGTTCTACT CCAGTGCTCA 
GAGGAACCAA ACCCCTGGTG CTGGCTCCTA GCTGAAGCAA TGAAACAACA GGCTCCTATC 
CTTAGTGTCC TGGCCTCATG TCTTCAGGAT GCCAGTACTA TTCCTTGTCT CTGTGTTTGG 
ATCATCACAT CTGTGGAGGA CAGTGTTGCA GCTGAAGCAA CAGAACACAT TCAGGGCTCA 
GTGGAAGGCC ACATATGGGA CCTTCAAGAC CTCTCAGTCA TCTGGAGAAC ATTATTAACA 
AGGCAGAAGA ACAAAACTCT CATCAGAGGT TTCCAACTTT TCTTTAAGGA TTCCCCACTA 
CTGTTGATGA TGGAGATGTA TGAACTGTGT ATGTTCTTCA AGAATTATAA AGAAGCTGAA 
GCTAAACTTC TGGAGTTCCA AAAGAGCTTT GAAACTGTTG AAACAGCAGC CACAAGGGTC 
CACACTACCA TCCCTGCCCC ATGGCTGAGG GAGCAGGTGT GTTTTCTTCT GAAGCTTATG 
CTGCAGCAGT GTGGGACCCA GTATGAACTG GGGAAGCTTT TACAGCTATT TGTTGGAATA 
GAAAATTTCT TCTCTGATGG TCCAGATGTG AAGAAGCTCT GTGTCCTTAG TGAAATTTTG 
AAGGACACAT CCATAGCCAT TAATCATGTA ATTATTAGCA GCCACAGCCT TGAGAATTTT 
CAGCATGAAT GTACATCTAT TTTGGAAAAA CTGGCGATGG AGGGACAGTT TGCTTTGGCC 
AGGAGAGTAG CAGAATTAGC TGAATTACCT GTGGACAACT TGGTTATTGA AGAGATGACA 
CAGGAAATGC AAACCTTAAA ACACATTGAC CAGTGGTCCC TGAAACAAGC AAGAATTGAC 
TTCTGGAAAA AATGTCATGA GAATTTTAAG AAAAATTCCA TTTCAAGCAA AGCAGCTTCT 
TCCTTTTTCT CAGCCCAGGC ACCTGAGTGC CCAACTGAAG AGGGTCGGAG CAGCAGTGAG 
GAGCGCCATC TGCTGCTCAC ATTGGCAGCA CACTGGCTTG CTCAGGAGGA TCTGGTGCCG 
GTGGCCGAAC TGGAGGAGCT AGAGAAGCAG ATCTGGCTCT GCCGCATTAC CCAATACACC 
CTCAGAGAAA AACAGGAGCA AGTAGAGCTC GGGTTTTCTC AACAGATTTC AACTAGTGGG 
GAACTTTCCT TTGATGGCAT AGCCAGTAAG TTTTCACTCT CCATGTTGGC TGTTCTGAAC 
ACATCAAAGT ACTTAGAACT CAGTGGCTTT CCATCCAAAG AGACATGTGA TAGCGGACTG 
GATTGGAAGG AGCAGGAGTC ACTGAACTTT CTGATTGGGC ACCTATTGGA TGATGGCTTT 
GTGCATGAAG CAAGTAGAGT GTGTCGGTAT TTTCATTTCT ATAACCAAGA TGTTGCCTTG 
GTGTTGCACT GCAGAGCACT GGCCTCAGGG GAAGCTAGTG TGGATGACCT GCACCCAGAG 
ATCCATGCTC TCCTACAAAG TGCTGAGCCG CTTGAGGAGG AGGAAGCTGG CATTCCACTA 
AGAAAAGTCC AGAGCAGTTC AAGTTTGGAT AGTCAGTCAT TTGTGATGCT GCCCCCCAGT 
GATGAAGTGG TGACTAATCT GGAAACTCTG ACAAGTAAAT GCCTCCATGG GAAGAACTAC 
TGTCGGCAGG TCCTGTGTCT GTATGAACTT GCCAAGGAGT TGGGCTGTTC CTACACAGAC 
GTGGCCTCTC AGGACGGGGA GGCCATGCTC CGGGCAATCT TGGCTTCCCA GCAGCCCGAC 
CGATGCAAGC GAGCCCAGGC TTTCATTAGC ACCCAGGGCC TCGAGGCAGA CACGGTGGCT 
GAACTTGTGG CTGAAGAGGT GACCCGGGAG CTGCTGACCC CACCGGAGGG AACAGGACAT 
CAGCAGATGT TTGCCCCAGC AGAGGAAAGC CGGACATTTC TTCAGCTGAC CACTCTGTGT 
CAAGACCGCA CACTGGTAGG CATGAAGTTG TTGGACAAGA TTTCGTCCGT TCCCCATGGA 
GAATTATCTT GCACCACAGA GCTGCTGATC CTGGCCCATC ACTGCTTCAC ACTGACATGC 
CACATGGAGG GCATCATCCG AGTCCTGCAA GCTGCCCGGC TGCTCACGGA CAAACACTTG 
GCCCCCAATG AAGAGTACGG GCTGATGGTA CGCCTCCTCA CCGGCATCGG AAGGTACAAC 
GAGATGACGT ACATATTTGA CTTGCTGCAT AATAAGCACT ATTTTGAAGT GCTGATGAGG 
AAGAAGTTGG ATCCGAGTGG CTCCCTGAAG ACCGCCCTAC TGGACTACAT CAAACGCTGC 
CGCCCTGGGG ACAGTGAGAA GCACAACATG ATCGCTCTGT GCTTCAGCAT GTGTCGGGAG 
ATTGGGGAGA ACCATGAGGC AGCTGCCCGC ATCCAGCTGA AACTGATCGA GTCTCAGCCC 
TGGGAGGACA GCCTCAAGGA CGGGCACCAC CTGAAGCAGC TGCTGCTGAA AGCCCTGACT 
CTGATGCTGG ATGCAGCCGA GAGCTATGCC AAGGACTCCT GTGTGCGACA GGCCCAGCAC 
TGTCACCGGC TCACCAAGTT GATAACACTG CAGATTCATT TTCTGAACAC TGGCCAGAAC 
ACAATGCTCA TCAACCTGGG CCGCCACCGG CTGATGGACT GTATCCTCGC TCTACCTCGG 
TTCTACCAGG CTTCTATTGT GGCCGAAGCC TATGACTTTG TTCCTGATTG GGCAGAAATC 
CTCTACCAAC AAGTGATTCT TAAAGGAGAC TTTAATTACC TGGAAGAATT TAAGCAGCAA 
AGGTTATTAA GATCCAGTAT ATTTGAAGAG ATTTCAAAAA AATATAAACA ACATCAGCCG 
ACAGACACGG CTGTGAAAAA CCTGAAGAAG TTACTCACAT ACTGTGAAGA TGTCTACTTG 
TACTACAAGC TCGCATATGA ACACAAATTT TATGACATTG TGAATGTGCT TCTGAAGGAC 
CCTCAGAGCG GCTGCTGTCT AAAGGACATG CTAGCAGGTT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013964965.1
CDS	12..7133
Translation	MAAGEAAARAAGCSSCGAETMERVLPMLLVPVPVEATGRLGSRAQLHREQEVLGSLTAAGSLQVLSLAPGGRSGGACCLNGPFRHFIWEDSHNGSSPTDKPKLLALSENYELFIYEFNLKDGRCDATILYSCSEETLQKLIEDQNISVSFLSLRILSFHNNTSLLFINKCIVLHIIFPERDAEIRVLNCFTLPLPAQAVDRIIDVQLCRGILFVLSSLGWIYIFDIVDGRHVAHVDLALHQEDMCNEQQQELAKISSFTSLKVSQDLDVVVIVSSFNSAVALNLNLYFRQHPGHLLCERTLEDIPIEGPEGIDEDDPVNSDYNMKLTKFSFQVDRSWKAQLSSLNEIIKSSKWEVPCCAPWFQNILHLESPESGYHSTSTPGWAFIPQDEIHSHYKFPQKDHANTGDPGRSWKIMNISELKEPVELTCMSVTGFSVLFTWAVEKMGCTIVLWDLETQGIQCFSLGKRCIPIDMSGDQQLCLVLTENGLSLILFGLTQEEFLNRLMIHGSASTVDSLCHLNGWGRCSIPIHALEAGIENRQLDTVDFFLKSKENLLNPSSKSSVPDQFDDFPSHLYLRNVEKLTPALDLLCSAIRESDSETQSKHFSEQLLNLTLSFLNKQIKELFVHNEELDEHLQKGVNILTSYINELRTFMIKFPWKIVDPVDEYDVSENVLKVRESDTWEKLSIEEVIANAILNNKIPEAQTFFRINNHSAQRLEELIRIGLNLVFDCLKKNNIKEASELLKNMGFDVKDQLLKICFYTTDKNIRDFLVEILKEKNYFSENEKRTIDFVHQVEKFYSGHFQENVQIQSFTRYWIKEQDFFKHKSVLDSLLKYDPKDKFNKQDHRIVLNWAQWWNQLTQESILLPRISPEEYKSCSPEALWSYLTAHHDWSSISLWIEEFQAQNSCVLLQQNKWPLLTVDVIDQNTCCNNYMRNEILDQLARLSPANCPFLEKKELHETHPWFEFLVQCRQVSSNLTDPKLIFQASLANAQILIPSNQASVSSMLLEGHTLLALATTMYAPGGVSQVVQDDENETCLKKVDPQLLKMALTPYPKLKTALFPQYTTSNVLPPDITLYHLIQSLLPFDSSRLFGWQSANTLAIGDAVSHLPHFSSPDLVNKYAIMERLNFAYYLQHGRPSFAFGTFLVQELTKSKTPKQLIQQVGNEAYVLGLSSFYIPSVGAACVCFLELLGLDSLKLRVDMKVANIILNYKCRNEDVQYSCIREALVEKLSKLAVGEKATVEELLILLEEGIWNNIQQQEIKRLSSESSSQWALAVQFCRLHNVKLSTSYLRECAKANDWLQFIIHSQLYNYHPAEVKSLLQYFSPVLQDHLRLAFENLTSVSKSKMDSGEVCSRSPQELQRNKEEMADFFQVLLQCSEEPNPWCWLLAEAMKQQAPILSVLASCLQDASTIPCLCVWIITSVEDSVAAEATEHIQGSVEGHIWDLQDLSVIWRTLLTRQKNKTLIRGFQLFFKDSPLLLMMEMYELCMFFKNYKEAEAKLLEFQKSFETVETAATRVHTTIPAPWLREQVCFLLKLMLQQCGTQYELGKLLQLFVGIENFFSDGPDVKKLCVLSEILKDTSIAINHVIISSHSLENFQHECTSILEKLAMEGQFALARRVAELAELPVDNLVIEEMTQEMQTLKHIDQWSLKQARIDFWKKCHENFKKNSISSKAASSFFSAQAPECPTEEGRSSSEERHLLLTLAAHWLAQEDLVPVAELEELEKQIWLCRITQYTLREKQEQVELGFSQQISTSGELSFDGIASKFSLSMLAVLNTSKYLELSGFPSKETCDSGLDWKEQESLNFLIGHLLDDGFVHEASRVCRYFHFYNQDVALVLHCRALASGEASVDDLHPEIHALLQSAEPLEEEEAGIPLRKVQSSSSLDSQSFVMLPPSDEVVTNLETLTSKCLHGKNYCRQVLCLYELAKELGCSYTDVASQDGEAMLRAILASQQPDRCKRAQAFISTQGLEADTVAELVAEEVTRELLTPPEGTGHQQMFAPAEESRTFLQLTTLCQDRTLVGMKLLDKISSVPHGELSCTTELLILAHHCFTLTCHMEGIIRVLQAARLLTDKHLAPNEEYGLMVRLLTGIGRYNEMTYIFDLLHNKHYFEVLMRKKLDPSGSLKTALLDYIKRCRPGDSEKHNMIALCFSMCREIGENHEAAARIQLKLIESQPWEDSLKDGHHLKQLLLKALTLMLDAAESYAKDSCVRQAQHCHRLTKLITLQIHFLNTGQNTMLINLGRHRLMDCILALPRFYQASIVAEAYDFVPDWAEILYQQVILKGDFNYLEEFKQQRLLRSSIFEEISKKYKQHQPTDTAVKNLKKLLTYCEDVYLYYKLAYEHKFYDIVNVLLKDPQSGCCLKDMLAG

Target ORF information:

RefSeq Version	XM_014109490.1
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris spastic paraplegia 11 (autosomal recessive) (SPG11), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014109490.1

ORF Insert Sequence:

ATGGCCGCAG GAGAGGCAGC TGCGAGAGCT GCTGGTTGCA GCAGCTGCGG CGCTGAAACT 
ATGGAACGGG TTCTACCTAT GCTGTTGGTG CCGGTCCCTG TGGAGGCAAC CGGGCGGTTG 
GGCTCCCGGG CGCAGTTGCA CAGAGAGCAG GAGGTTCTGG GGAGCCTAAC AGCTGCAGGC 
AGCCTGCAGG TGCTTTCCTT GGCGCCGGGC GGCCGGAGCG GCGGGGCCTG CTGCCTCAAT 
GGCCCCTTTC GGCACTTTAT ATGGGAGGAT TCTCATAACG GCAGCTCACC AACAGACAAG 
CCCAAACTTC TTGCTCTTAG TGAAAACTAT GAACTGTTTA TCTATGAATT TAATTTGAAA 
GATGGAAGAT GTGATGCAAC CATTTTGTAT AGCTGTAGTG AGGAGACATT GCAGAAGCTC 
ATTGAAGATC AAAATATCAG TGTTTCCTTT TTATCTTTGA GAATTCTGTC ATTTCACAAT 
AACACATCAT TATTGTTCAT CAATAAATGT ATTGTCCTAC ACATTATATT TCCTGAAAGA 
GATGCTGAGA TCAGAGTACT CAACTGTTTC ACGTTACCCT TGCCTGCACA GGCAGTGGAC 
AGGATCATTG ATGTGCAGCT CTGCAGAGGA ATTCTTTTTG TTTTGAGTAG TTTAGGCTGG 
ATCTACATTT TTGACATTGT GGATGGAAGA CATGTAGCTC ACGTGGATTT AGCACTTCAC 
CAAGAAGATA TGTGTAATGA ACAGCAACAG GAACTAGCCA AGATTTCTTC CTTTACTTCA 
CTGAAAGTGT CTCAAGACCT AGATGTTGTA GTGATTGTCA GCTCCTTCAA CTCTGCAGTT 
GCTCTTAACC TAAACTTGTA TTTCAGGCAA CACCCAGGAC ACCTACTGTG TGAAAGAACT 
CTAGAAGACA TTCCTATTGA AGGCCCTGAG GGGATCGATG AAGATGATCC TGTTAATTCT 
GACTACAACA TGAAACTGAC CAAGTTTTCC TTTCAAGTTG ATAGGTCTTG GAAAGCCCAG 
CTATCATCAC TGAATGAAAT AATAAAGAGC TCCAAATGGG AGGTTCCCTG TTGTGCTCCA 
TGGTTCCAGA ATATTTTGCA TTTGGAGTCA CCGGAATCTG GTTACCACAG TACTAGTACA 
CCAGGCTGGG CCTTCATCCC ACAGGATGAA ATTCATAGCC ACTACAAGTT TCCACAGAAA 
GATCATGCCA ACACCGGTGA CCCAGGAAGA TCATGGAAAA TAATGAACAT TAGTGAACTA 
AAGGAACCTG TTGAGCTTAC ATGTATGTCT GTGACAGGAT TCAGTGTACT GTTTACTTGG 
GCAGTGGAAA AGATGGGCTG CACCATTGTC CTTTGGGATT TGGAGACCCA GGGTATCCAG 
TGTTTTTCCC TTGGCAAAAG GTGTATTCCT ATAGATATGA GTGGAGACCA GCAGCTGTGC 
CTTGTTTTGA CAGAAAATGG ACTCTCCCTG ATTTTGTTTG GATTGACTCA GGAGGAGTTT 
TTAAATAGAC TAATGATCCA TGGAAGTGCC AGCACTGTGG ACTCTCTTTG CCATCTCAAT 
GGTTGGGGGA GGTGCTCAAT CCCCATACAT GCGCTGGAGG CTGGGATAGA AAATCGTCAG 
CTGGACACCG TAGATTTCTT TTTGAAGAGC AAAGAAAATC TTTTGAATCC ATCCTCAAAA 
TCTTCCGTAC CGGATCAGTT TGATGACTTT CCATCTCATT TATATTTAAG AAATGTGGAA 
AAGCTGACAC CAGCATTGGA TTTACTTTGC TCAGCAATTA GAGAAAGTGA TTCTGAAACC 
CAAAGCAAAC ACTTTTCAGA ACAGTTGCTT AACCTTACAC TGTCTTTTCT TAACAAACAA 
ATAAAGGAGC TTTTTGTTCA CAATGAAGAA TTAGATGAAC ATCTACAGAA AGGAGTGAAC 
ATTTTGACCA GCTACATTAA TGAACTTCGA ACCTTCATGA TAAAATTTCC TTGGAAGATA 
GTAGACCCAG TAGATGAATA TGATGTAAGT GAAAATGTCC TCAAAGTAAG AGAGAGTGAT 
ACATGGGAGA AACTCAGCAT TGAGGAAGTT ATTGCCAATG CTATTTTAAA CAACAAAATA 
CCAGAGGCAC AGACTTTCTT CAGGATTAAC AATCATTCTG CTCAAAGACT TGAGGAGCTG 
ATTAGAATAG GCCTAAATTT GGTCTTTGAC TGTTTGAAAA AGAACAATAT AAAGGAAGCA 
TCTGAACTTT TGAAGAATAT GGGCTTTGAT GTAAAAGACC AGTTGCTCAA GATATGTTTC 
TATACAACTG ATAAAAATAT ACGGGACTTT TTGGTGGAAA TTTTAAAAGA AAAAAATTAT 
TTTTCTGAAA ATGAAAAAAG AACTATAGAC TTCGTGCATC AAGTTGAGAA GTTTTATTCA 
GGACATTTTC AAGAAAATGT GCAGATCCAG TCCTTTACCA GGTATTGGAT AAAGGAACAG 
GATTTTTTCA AGCACAAATC TGTTTTGGAC TCACTCCTGA AATATGATCC TAAAGATAAA 
TTTAACAAAC AGGACCATAG AATTGTGTTA AATTGGGCTC AGTGGTGGAA TCAACTAACA 
CAAGAATCCA TCCTTCTCCC CAGGATAAGT CCAGAAGAAT ACAAATCATG TTCTCCTGAA 
GCCCTTTGGA GCTATCTCAC AGCCCATCAT GATTGGTCAA GCATTAGCTT GTGGATTGAA 
GAATTTCAGG CCCAGAACAG TTGTGTTTTA CTGCAGCAGA ACAAATGGCC CCTTCTGACT 
GTTGATGTTA TAGACCAGAA TACTTGCTGC AACAACTACA TGAGGAATGA AATTTTAGAT 
CAGCTGGCCA GACTTAGTCC TGCAAATTGT CCCTTTTTGG AAAAAAAAGA ATTACATGAA 
ACTCACCCTT GGTTTGAATT TTTGGTTCAG TGTCGACAGG TTTCCAGTAA TTTAACAGAT 
CCCAAACTGA TCTTCCAGGC TAGCCTTGCA AACGCTCAGA TACTGATTCC CAGCAACCAG 
GCCAGTGTAA GCAGTATGCT ATTGGAAGGA CATACTCTCC TGGCCCTTGC TACCACAATG 
TATGCCCCTG GCGGTGTCAG CCAGGTTGTT CAGGATGATG AAAATGAGAC CTGTTTGAAG 
AAAGTAGATC CCCAGTTACT GAAGATGGCA TTAACTCCTT ATCCCAAGCT AAAAACTGCT 
CTCTTCCCAC AGTACACTAC CTCTAATGTT CTGCCGCCTG ATATTACGCT TTACCACCTT 
ATTCAGTCAC TATTGCCCTT TGACTCTAGC AGATTGTTTG GCTGGCAGTC TGCCAACACA 
CTGGCTATTG GAGATGCAGT GAGTCATCTC CCACATTTTT CTAGCCCTGA TCTGGTTAAT 
AAATATGCTA TCATGGAACG TCTGAATTTT GCATATTATT TACAACATGG ACGGCCGTCA 
TTTGCATTTG GTACTTTTCT GGTCCAGGAA TTAACCAAGA GCAAGACTCC CAAGCAGCTG 
ATCCAGCAAG TAGGCAATGA AGCTTATGTT TTAGGGCTCT CCTCCTTCTA CATTCCATCA 
GTAGGAGCTG CCTGTGTTTG TTTCTTAGAA CTGCTTGGCC TCGATAGCCT CAAGCTCAGA 
GTTGACATGA AAGTGGCCAA TATAATTTTG AACTACAAAT GTAGAAATGA AGATGTTCAG 
TACAGCTGTA TCAGAGAGGC TCTAGTTGAA AAACTATCTA AACTGGCTGT GGGTGAAAAG 
GCCACCGTAG AGGAATTGCT TATTCTCTTA GAAGAAGGTA TATGGAACAA CATTCAGCAG 
CAAGAGATAA AGAGGTTATC TAGTGAATCT AGCAGCCAGT GGGCATTAGC AGTACAGTTC 
TGCAGGCTTC ACAATGTGAA GCTGAGCACG TCTTACCTTA GAGAATGTGC CAAAGCAAAT 
GACTGGTTAC AGTTCATTAT TCACAGCCAA CTCTATAACT ATCACCCAGC GGAGGTGAAA 
TCTCTTCTTC AGTATTTCAG TCCTGTCCTT CAAGACCACT TAAGACTAGC TTTTGAGAAC 
CTGACCTCAG TGTCTAAATC CAAAATGGAT AGTGGTGAAG TCTGCAGCAG GTCTCCCCAG 
GAACTCCAGA GAAACAAAGA AGAAATGGCT GATTTCTTTC AAGTTCTACT CCAGTGCTCA 
GAGGAACCAA ACCCCTGGTG CTGGCTCCTA GCTGAAGCAA TGAAACAACA GGCTCCTATC 
CTTAGTGTCC TGGCCTCATG TCTTCAGGAT GCCAGTACTA TTCCTTGTCT CTGTGTTTGG 
ATCATCACAT CTGTGGAGGA CAGTGTTGCA GCTGAAGCAA CAGAACACAT TCAGGGCTCA 
GTGGAAGGCC ACATATGGGA CCTTCAAGAC CTCTCAGTCA TCTGGAGAAC ATTATTAACA 
AGGCAGAAGA ACAAAACTCT CATCAGAGGT TTCCAACTTT TCTTTAAGGA TTCCCCACTA 
CTGTTGATGA TGGAGATGTA TGAACTGTGT ATGTTCTTCA AGAATTATAA AGAAGCTGAA 
GCTAAACTTC TGGAGTTCCA AAAGAGCTTT GAAACTGTTG AAACAGCAGC CACAAGGGTC 
CACACTACCA TCCCTGCCCC ATGGCTGAGG GAGCAGGTGT GTTTTCTTCT GAAGCTTATG 
CTGCAGCAGT GTGGGACCCA GTATGAACTG GGGAAGCTTT TACAGCTATT TGTTGGAATA 
GAAAATTTCT TCTCTGATGG TCCAGATGTG AAGAAGCTCT GTGTCCTTAG TGAAATTTTG 
AAGGACACAT CCATAGCCAT TAATCATGTA ATTATTAGCA GCCACAGCCT TGAGAATTTT 
CAGCATGAAT GTACATCTAT TTTGGAAAAA CTGGCGATGG AGGGACAGTT TGCTTTGGCC 
AGGAGAGTAG CAGAATTAGC TGAATTACCT GTGGACAACT TGGTTATTGA AGAGATGACA 
CAGGAAATGC AAACCTTAAA ACACATTGAC CAGTGGTCCC TGAAACAAGC AAGAATTGAC 
TTCTGGAAAA AATGTCATGA GAATTTTAAG AAAAATTCCA TTTCAAGCAA AGCAGCTTCT 
TCCTTTTTCT CAGCCCAGGC ACCTGAGTGC CCAACTGAAG AGGGTCGGAG CAGCAGTGAG 
GAGCGCCATC TGCTGCTCAC ATTGGCAGCA CACTGGCTTG CTCAGGAGGA TCTGGTGCCG 
GTGGCCGAAC TGGAGGAGCT AGAGAAGCAG ATCTGGCTCT GCCGCATTAC CCAATACACC 
CTCAGAGAAA AACAGGAGCA AGTAGAGCTC GGGTTTTCTC AACAGATTTC AACTAGTGGG 
GAACTTTCCT TTGATGGCAT AGCCAGTAAG TTTTCACTCT CCATGTTGGC TGTTCTGAAC 
ACATCAAAGT ACTTAGAACT CAGTGGCTTT CCATCCAAAG AGACATGTGA TAGCGGACTG 
GATTGGAAGG AGCAGGAGTC ACTGAACTTT CTGATTGGGC ACCTATTGGA TGATGGCTTT 
GTGCATGAAG CAAGTAGAGT GTGTCGGTAT TTTCATTTCT ATAACCAAGA TGTTGCCTTG 
GTGTTGCACT GCAGAGCACT GGCCTCAGGG GAAGCTAGTG TGGATGACCT GCACCCAGAG 
ATCCATGCTC TCCTACAAAG TGCTGAGCCG CTTGAGGAGG AGGAAGCTGG CATTCCACTA 
AGAAAAGTCC AGAGCAGTTC AAGTTTGGAT AGTCAGTCAT TTGTGATGCT GCCCCCCAGT 
GATGAAGTGG TGACTAATCT GGAAACTCTG ACAAGTAAAT GCCTCCATGG GAAGAACTAC 
TGTCGGCAGG TCCTGTGTCT GTATGAACTT GCCAAGGAGT TGGGCTGTTC CTACACAGAC 
GTGGCCTCTC AGGACGGGGA GGCCATGCTC CGGGCAATCT TGGCTTCCCA GCAGCCCGAC 
CGATGCAAGC GAGCCCAGGC TTTCATTAGC ACCCAGGGCC TCGAGGCAGA CACGGTGGCT 
GAACTTGTGG CTGAAGAGGT GACCCGGGAG CTGCTGACCC CACCGGAGGG AACAGGACAT 
CAGCAGATGT TTGCCCCAGC AGAGGAAAGC CGGACATTTC TTCAGCTGAC CACTCTGTGT 
CAAGACCGCA CACTGGTAGG CATGAAGTTG TTGGACAAGA TTTCGTCCGT TCCCCATGGA 
GAATTATCTT GCACCACAGA GCTGCTGATC CTGGCCCATC ACTGCTTCAC ACTGACATGC 
CACATGGAGG GCATCATCCG AGTCCTGCAA GCTGCCCGGC TGCTCACGGA CAAACACTTG 
GCCCCCAATG AAGAGTACGG GCTGATGGTA CGCCTCCTCA CCGGCATCGG AAGGTACAAC 
GAGATGACGT ACATATTTGA CTTGCTGCAT AATAAGCACT ATTTTGAAGT GCTGATGAGG 
AAGAAGTTGG ATCCGAGTGG CTCCCTGAAG ACCGCCCTAC TGGACTACAT CAAACGCTGC 
CGCCCTGGGG ACAGTGAGAA GCACAACATG ATCGCTCTGT GCTTCAGCAT GTGTCGGGAG 
ATTGGGGAGA ACCATGAGGC AGCTGCCCGC ATCCAGCTGA AACTGATCGA GTCTCAGCCC 
TGGGAGGACA GCCTCAAGGA CGGGCACCAC CTGAAGCAGC TGCTGCTGAA AGCCCTGACT 
CTGATGCTGG ATGCAGCCGA GAGCTATGCC AAGGACTCCT GTGTGCGACA GGCCCAGCAC 
TGTCACCGGC TCACCAAGTT GATAACACTG CAGATTCATT TTCTGAACAC TGGCCAGAAC 
ACAATGCTCA TCAACCTGGG CCGCCACCGG CTGATGGACT GTATCCTCGC TCTACCTCGG 
TTCTACCAGG CTTCTATTGT GGCCGAAGCC TATGACTTTG TTCCTGATTG GGCAGAAATC 
CTCTACCAAC AAGTGATTCT TAAAGGAGAC TTTAATTACC TGGAAGAATT TAAGCAGCAA 
AGGTTATTAA GATCCAGTAT ATTTGAAGAG ATTTCAAAAA AATATAAACA ACATCAGCCG 
ACAGACACGG CTGTGAAAAA CCTGAAGAAG TTACTCACAT ACTGTGAAGA TGTCTACTTG 
TACTACAAGC TCGCATATGA ACACAAATTT TATGACATTG TGAATGTGCT TCTGAAGGAC 
CCTCAGAGCG GCTGCTGTCT AAAGGACATG CTAGCAGGTT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SPG11 ( XM_014109490.2 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_013964965.1 Canis lupus familiaris SPG11, spatacsin vesicle trafficking associated (SPG11), transcript variant X2, mRNA.

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CloneID	OCe44278
Clone ID Related Accession (Same CDS sequence)	XM_014109490.1 , XM_014109490.2
Accession Version	XM_014109490.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7122bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1504454400000
Organism	Canis lupus familiaris(dog)
Product	spatacsin isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006612.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_014109490.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCGCAG GAGAGGCAGC TGCGAGAGCT GCTGGTTGCA GCAGCTGCGG CGCTGAAACT 
ATGGAACGGG TTCTACCTAT GCTGTTGGTG CCGGTCCCTG TGGAGGCAAC CGGGCGGTTG 
GGCTCCCGGG CGCAGTTGCA CAGAGAGCAG GAGGTTCTGG GGAGCCTAAC AGCTGCAGGC 
AGCCTGCAGG TGCTTTCCTT GGCGCCGGGC GGCCGGAGCG GCGGGGCCTG CTGCCTCAAT 
GGCCCCTTTC GGCACTTTAT ATGGGAGGAT TCTCATAACG GCAGCTCACC AACAGACAAG 
CCCAAACTTC TTGCTCTTAG TGAAAACTAT GAACTGTTTA TCTATGAATT TAATTTGAAA 
GATGGAAGAT GTGATGCAAC CATTTTGTAT AGCTGTAGTG AGGAGACATT GCAGAAGCTC 
ATTGAAGATC AAAATATCAG TGTTTCCTTT TTATCTTTGA GAATTCTGTC ATTTCACAAT 
AACACATCAT TATTGTTCAT CAATAAATGT ATTGTCCTAC ACATTATATT TCCTGAAAGA 
GATGCTGAGA TCAGAGTACT CAACTGTTTC ACGTTACCCT TGCCTGCACA GGCAGTGGAC 
AGGATCATTG ATGTGCAGCT CTGCAGAGGA ATTCTTTTTG TTTTGAGTAG TTTAGGCTGG 
ATCTACATTT TTGACATTGT GGATGGAAGA CATGTAGCTC ACGTGGATTT AGCACTTCAC 
CAAGAAGATA TGTGTAATGA ACAGCAACAG GAACTAGCCA AGATTTCTTC CTTTACTTCA 
CTGAAAGTGT CTCAAGACCT AGATGTTGTA GTGATTGTCA GCTCCTTCAA CTCTGCAGTT 
GCTCTTAACC TAAACTTGTA TTTCAGGCAA CACCCAGGAC ACCTACTGTG TGAAAGAACT 
CTAGAAGACA TTCCTATTGA AGGCCCTGAG GGGATCGATG AAGATGATCC TGTTAATTCT 
GACTACAACA TGAAACTGAC CAAGTTTTCC TTTCAAGTTG ATAGGTCTTG GAAAGCCCAG 
CTATCATCAC TGAATGAAAT AATAAAGAGC TCCAAATGGG AGGTTCCCTG TTGTGCTCCA 
TGGTTCCAGA ATATTTTGCA TTTGGAGTCA CCGGAATCTG GTTACCACAG TACTAGTACA 
CCAGGCTGGG CCTTCATCCC ACAGGATGAA ATTCATAGCC ACTACAAGTT TCCACAGAAA 
GATCATGCCA ACACCGGTGA CCCAGGAAGA TCATGGAAAA TAATGAACAT TAGTGAACTA 
AAGGAACCTG TTGAGCTTAC ATGTATGTCT GTGACAGGAT TCAGTGTACT GTTTACTTGG 
GCAGTGGAAA AGATGGGCTG CACCATTGTC CTTTGGGATT TGGAGACCCA GGGTATCCAG 
TGTTTTTCCC TTGGCAAAAG GTGTATTCCT ATAGATATGA GTGGAGACCA GCAGCTGTGC 
CTTGTTTTGA CAGAAAATGG ACTCTCCCTG ATTTTGTTTG GATTGACTCA GGAGGAGTTT 
TTAAATAGAC TAATGATCCA TGGAAGTGCC AGCACTGTGG ACTCTCTTTG CCATCTCAAT 
GGTTGGGGGA GGTGCTCAAT CCCCATACAT GCGCTGGAGG CTGGGATAGA AAATCGTCAG 
CTGGACACCG TAGATTTCTT TTTGAAGAGC AAAGAAAATC TTTTGAATCC ATCCTCAAAA 
TCTTCCGTAC CGGATCAGTT TGATGACTTT CCATCTCATT TATATTTAAG AAATGTGGAA 
AAGCTGACAC CAGCATTGGA TTTACTTTGC TCAGCAATTA GAGAAAGTGA TTCTGAAACC 
CAAAGCAAAC ACTTTTCAGA ACAGTTGCTT AACCTTACAC TGTCTTTTCT TAACAAACAA 
ATAAAGGAGC TTTTTGTTCA CAATGAAGAA TTAGATGAAC ATCTACAGAA AGGAGTGAAC 
ATTTTGACCA GCTACATTAA TGAACTTCGA ACCTTCATGA TAAAATTTCC TTGGAAGATA 
GTAGACCCAG TAGATGAATA TGATGTAAGT GAAAATGTCC TCAAAGTAAG AGAGAGTGAT 
ACATGGGAGA AACTCAGCAT TGAGGAAGTT ATTGCCAATG CTATTTTAAA CAACAAAATA 
CCAGAGGCAC AGACTTTCTT CAGGATTAAC AATCATTCTG CTCAAAGACT TGAGGAGCTG 
ATTAGAATAG GCCTAAATTT GGTCTTTGAC TGTTTGAAAA AGAACAATAT AAAGGAAGCA 
TCTGAACTTT TGAAGAATAT GGGCTTTGAT GTAAAAGACC AGTTGCTCAA GATATGTTTC 
TATACAACTG ATAAAAATAT ACGGGACTTT TTGGTGGAAA TTTTAAAAGA AAAAAATTAT 
TTTTCTGAAA ATGAAAAAAG AACTATAGAC TTCGTGCATC AAGTTGAGAA GTTTTATTCA 
GGACATTTTC AAGAAAATGT GCAGATCCAG TCCTTTACCA GGTATTGGAT AAAGGAACAG 
GATTTTTTCA AGCACAAATC TGTTTTGGAC TCACTCCTGA AATATGATCC TAAAGATAAA 
TTTAACAAAC AGGACCATAG AATTGTGTTA AATTGGGCTC AGTGGTGGAA TCAACTAACA 
CAAGAATCCA TCCTTCTCCC CAGGATAAGT CCAGAAGAAT ACAAATCATG TTCTCCTGAA 
GCCCTTTGGA GCTATCTCAC AGCCCATCAT GATTGGTCAA GCATTAGCTT GTGGATTGAA 
GAATTTCAGG CCCAGAACAG TTGTGTTTTA CTGCAGCAGA ACAAATGGCC CCTTCTGACT 
GTTGATGTTA TAGACCAGAA TACTTGCTGC AACAACTACA TGAGGAATGA AATTTTAGAT 
CAGCTGGCCA GACTTAGTCC TGCAAATTGT CCCTTTTTGG AAAAAAAAGA ATTACATGAA 
ACTCACCCTT GGTTTGAATT TTTGGTTCAG TGTCGACAGG TTTCCAGTAA TTTAACAGAT 
CCCAAACTGA TCTTCCAGGC TAGCCTTGCA AACGCTCAGA TACTGATTCC CAGCAACCAG 
GCCAGTGTAA GCAGTATGCT ATTGGAAGGA CATACTCTCC TGGCCCTTGC TACCACAATG 
TATGCCCCTG GCGGTGTCAG CCAGGTTGTT CAGGATGATG AAAATGAGAC CTGTTTGAAG 
AAAGTAGATC CCCAGTTACT GAAGATGGCA TTAACTCCTT ATCCCAAGCT AAAAACTGCT 
CTCTTCCCAC AGTACACTAC CTCTAATGTT CTGCCGCCTG ATATTACGCT TTACCACCTT 
ATTCAGTCAC TATTGCCCTT TGACTCTAGC AGATTGTTTG GCTGGCAGTC TGCCAACACA 
CTGGCTATTG GAGATGCAGT GAGTCATCTC CCACATTTTT CTAGCCCTGA TCTGGTTAAT 
AAATATGCTA TCATGGAACG TCTGAATTTT GCATATTATT TACAACATGG ACGGCCGTCA 
TTTGCATTTG GTACTTTTCT GGTCCAGGAA TTAACCAAGA GCAAGACTCC CAAGCAGCTG 
ATCCAGCAAG TAGGCAATGA AGCTTATGTT TTAGGGCTCT CCTCCTTCTA CATTCCATCA 
GTAGGAGCTG CCTGTGTTTG TTTCTTAGAA CTGCTTGGCC TCGATAGCCT CAAGCTCAGA 
GTTGACATGA AAGTGGCCAA TATAATTTTG AACTACAAAT GTAGAAATGA AGATGTTCAG 
TACAGCTGTA TCAGAGAGGC TCTAGTTGAA AAACTATCTA AACTGGCTGT GGGTGAAAAG 
GCCACCGTAG AGGAATTGCT TATTCTCTTA GAAGAAGGTA TATGGAACAA CATTCAGCAG 
CAAGAGATAA AGAGGTTATC TAGTGAATCT AGCAGCCAGT GGGCATTAGC AGTACAGTTC 
TGCAGGCTTC ACAATGTGAA GCTGAGCACG TCTTACCTTA GAGAATGTGC CAAAGCAAAT 
GACTGGTTAC AGTTCATTAT TCACAGCCAA CTCTATAACT ATCACCCAGC GGAGGTGAAA 
TCTCTTCTTC AGTATTTCAG TCCTGTCCTT CAAGACCACT TAAGACTAGC TTTTGAGAAC 
CTGACCTCAG TGTCTAAATC CAAAATGGAT AGTGGTGAAG TCTGCAGCAG GTCTCCCCAG 
GAACTCCAGA GAAACAAAGA AGAAATGGCT GATTTCTTTC AAGTTCTACT CCAGTGCTCA 
GAGGAACCAA ACCCCTGGTG CTGGCTCCTA GCTGAAGCAA TGAAACAACA GGCTCCTATC 
CTTAGTGTCC TGGCCTCATG TCTTCAGGAT GCCAGTACTA TTCCTTGTCT CTGTGTTTGG 
ATCATCACAT CTGTGGAGGA CAGTGTTGCA GCTGAAGCAA CAGAACACAT TCAGGGCTCA 
GTGGAAGGCC ACATATGGGA CCTTCAAGAC CTCTCAGTCA TCTGGAGAAC ATTATTAACA 
AGGCAGAAGA ACAAAACTCT CATCAGAGGT TTCCAACTTT TCTTTAAGGA TTCCCCACTA 
CTGTTGATGA TGGAGATGTA TGAACTGTGT ATGTTCTTCA AGAATTATAA AGAAGCTGAA 
GCTAAACTTC TGGAGTTCCA AAAGAGCTTT GAAACTGTTG AAACAGCAGC CACAAGGGTC 
CACACTACCA TCCCTGCCCC ATGGCTGAGG GAGCAGGTGT GTTTTCTTCT GAAGCTTATG 
CTGCAGCAGT GTGGGACCCA GTATGAACTG GGGAAGCTTT TACAGCTATT TGTTGGAATA 
GAAAATTTCT TCTCTGATGG TCCAGATGTG AAGAAGCTCT GTGTCCTTAG TGAAATTTTG 
AAGGACACAT CCATAGCCAT TAATCATGTA ATTATTAGCA GCCACAGCCT TGAGAATTTT 
CAGCATGAAT GTACATCTAT TTTGGAAAAA CTGGCGATGG AGGGACAGTT TGCTTTGGCC 
AGGAGAGTAG CAGAATTAGC TGAATTACCT GTGGACAACT TGGTTATTGA AGAGATGACA 
CAGGAAATGC AAACCTTAAA ACACATTGAC CAGTGGTCCC TGAAACAAGC AAGAATTGAC 
TTCTGGAAAA AATGTCATGA GAATTTTAAG AAAAATTCCA TTTCAAGCAA AGCAGCTTCT 
TCCTTTTTCT CAGCCCAGGC ACCTGAGTGC CCAACTGAAG AGGGTCGGAG CAGCAGTGAG 
GAGCGCCATC TGCTGCTCAC ATTGGCAGCA CACTGGCTTG CTCAGGAGGA TCTGGTGCCG 
GTGGCCGAAC TGGAGGAGCT AGAGAAGCAG ATCTGGCTCT GCCGCATTAC CCAATACACC 
CTCAGAGAAA AACAGGAGCA AGTAGAGCTC GGGTTTTCTC AACAGATTTC AACTAGTGGG 
GAACTTTCCT TTGATGGCAT AGCCAGTAAG TTTTCACTCT CCATGTTGGC TGTTCTGAAC 
ACATCAAAGT ACTTAGAACT CAGTGGCTTT CCATCCAAAG AGACATGTGA TAGCGGACTG 
GATTGGAAGG AGCAGGAGTC ACTGAACTTT CTGATTGGGC ACCTATTGGA TGATGGCTTT 
GTGCATGAAG CAAGTAGAGT GTGTCGGTAT TTTCATTTCT ATAACCAAGA TGTTGCCTTG 
GTGTTGCACT GCAGAGCACT GGCCTCAGGG GAAGCTAGTG TGGATGACCT GCACCCAGAG 
ATCCATGCTC TCCTACAAAG TGCTGAGCCG CTTGAGGAGG AGGAAGCTGG CATTCCACTA 
AGAAAAGTCC AGAGCAGTTC AAGTTTGGAT AGTCAGTCAT TTGTGATGCT GCCCCCCAGT 
GATGAAGTGG TGACTAATCT GGAAACTCTG ACAAGTAAAT GCCTCCATGG GAAGAACTAC 
TGTCGGCAGG TCCTGTGTCT GTATGAACTT GCCAAGGAGT TGGGCTGTTC CTACACAGAC 
GTGGCCTCTC AGGACGGGGA GGCCATGCTC CGGGCAATCT TGGCTTCCCA GCAGCCCGAC 
CGATGCAAGC GAGCCCAGGC TTTCATTAGC ACCCAGGGCC TCGAGGCAGA CACGGTGGCT 
GAACTTGTGG CTGAAGAGGT GACCCGGGAG CTGCTGACCC CACCGGAGGG AACAGGACAT 
CAGCAGATGT TTGCCCCAGC AGAGGAAAGC CGGACATTTC TTCAGCTGAC CACTCTGTGT 
CAAGACCGCA CACTGGTAGG CATGAAGTTG TTGGACAAGA TTTCGTCCGT TCCCCATGGA 
GAATTATCTT GCACCACAGA GCTGCTGATC CTGGCCCATC ACTGCTTCAC ACTGACATGC 
CACATGGAGG GCATCATCCG AGTCCTGCAA GCTGCCCGGC TGCTCACGGA CAAACACTTG 
GCCCCCAATG AAGAGTACGG GCTGATGGTA CGCCTCCTCA CCGGCATCGG AAGGTACAAC 
GAGATGACGT ACATATTTGA CTTGCTGCAT AATAAGCACT ATTTTGAAGT GCTGATGAGG 
AAGAAGTTGG ATCCGAGTGG CTCCCTGAAG ACCGCCCTAC TGGACTACAT CAAACGCTGC 
CGCCCTGGGG ACAGTGAGAA GCACAACATG ATCGCTCTGT GCTTCAGCAT GTGTCGGGAG 
ATTGGGGAGA ACCATGAGGC AGCTGCCCGC ATCCAGCTGA AACTGATCGA GTCTCAGCCC 
TGGGAGGACA GCCTCAAGGA CGGGCACCAC CTGAAGCAGC TGCTGCTGAA AGCCCTGACT 
CTGATGCTGG ATGCAGCCGA GAGCTATGCC AAGGACTCCT GTGTGCGACA GGCCCAGCAC 
TGTCACCGGC TCACCAAGTT GATAACACTG CAGATTCATT TTCTGAACAC TGGCCAGAAC 
ACAATGCTCA TCAACCTGGG CCGCCACCGG CTGATGGACT GTATCCTCGC TCTACCTCGG 
TTCTACCAGG CTTCTATTGT GGCCGAAGCC TATGACTTTG TTCCTGATTG GGCAGAAATC 
CTCTACCAAC AAGTGATTCT TAAAGGAGAC TTTAATTACC TGGAAGAATT TAAGCAGCAA 
AGGTTATTAA GATCCAGTAT ATTTGAAGAG ATTTCAAAAA AATATAAACA ACATCAGCCG 
ACAGACACGG CTGTGAAAAA CCTGAAGAAG TTACTCACAT ACTGTGAAGA TGTCTACTTG 
TACTACAAGC TCGCATATGA ACACAAATTT TATGACATTG TGAATGTGCT TCTGAAGGAC 
CCTCAGAGCG GCTGCTGTCT AAAGGACATG CTAGCAGGTT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013964965.1
CDS	21..7142
Translation	MAAGEAAARAAGCSSCGAETMERVLPMLLVPVPVEATGRLGSRAQLHREQEVLGSLTAAGSLQVLSLAPGGRSGGACCLNGPFRHFIWEDSHNGSSPTDKPKLLALSENYELFIYEFNLKDGRCDATILYSCSEETLQKLIEDQNISVSFLSLRILSFHNNTSLLFINKCIVLHIIFPERDAEIRVLNCFTLPLPAQAVDRIIDVQLCRGILFVLSSLGWIYIFDIVDGRHVAHVDLALHQEDMCNEQQQELAKISSFTSLKVSQDLDVVVIVSSFNSAVALNLNLYFRQHPGHLLCERTLEDIPIEGPEGIDEDDPVNSDYNMKLTKFSFQVDRSWKAQLSSLNEIIKSSKWEVPCCAPWFQNILHLESPESGYHSTSTPGWAFIPQDEIHSHYKFPQKDHANTGDPGRSWKIMNISELKEPVELTCMSVTGFSVLFTWAVEKMGCTIVLWDLETQGIQCFSLGKRCIPIDMSGDQQLCLVLTENGLSLILFGLTQEEFLNRLMIHGSASTVDSLCHLNGWGRCSIPIHALEAGIENRQLDTVDFFLKSKENLLNPSSKSSVPDQFDDFPSHLYLRNVEKLTPALDLLCSAIRESDSETQSKHFSEQLLNLTLSFLNKQIKELFVHNEELDEHLQKGVNILTSYINELRTFMIKFPWKIVDPVDEYDVSENVLKVRESDTWEKLSIEEVIANAILNNKIPEAQTFFRINNHSAQRLEELIRIGLNLVFDCLKKNNIKEASELLKNMGFDVKDQLLKICFYTTDKNIRDFLVEILKEKNYFSENEKRTIDFVHQVEKFYSGHFQENVQIQSFTRYWIKEQDFFKHKSVLDSLLKYDPKDKFNKQDHRIVLNWAQWWNQLTQESILLPRISPEEYKSCSPEALWSYLTAHHDWSSISLWIEEFQAQNSCVLLQQNKWPLLTVDVIDQNTCCNNYMRNEILDQLARLSPANCPFLEKKELHETHPWFEFLVQCRQVSSNLTDPKLIFQASLANAQILIPSNQASVSSMLLEGHTLLALATTMYAPGGVSQVVQDDENETCLKKVDPQLLKMALTPYPKLKTALFPQYTTSNVLPPDITLYHLIQSLLPFDSSRLFGWQSANTLAIGDAVSHLPHFSSPDLVNKYAIMERLNFAYYLQHGRPSFAFGTFLVQELTKSKTPKQLIQQVGNEAYVLGLSSFYIPSVGAACVCFLELLGLDSLKLRVDMKVANIILNYKCRNEDVQYSCIREALVEKLSKLAVGEKATVEELLILLEEGIWNNIQQQEIKRLSSESSSQWALAVQFCRLHNVKLSTSYLRECAKANDWLQFIIHSQLYNYHPAEVKSLLQYFSPVLQDHLRLAFENLTSVSKSKMDSGEVCSRSPQELQRNKEEMADFFQVLLQCSEEPNPWCWLLAEAMKQQAPILSVLASCLQDASTIPCLCVWIITSVEDSVAAEATEHIQGSVEGHIWDLQDLSVIWRTLLTRQKNKTLIRGFQLFFKDSPLLLMMEMYELCMFFKNYKEAEAKLLEFQKSFETVETAATRVHTTIPAPWLREQVCFLLKLMLQQCGTQYELGKLLQLFVGIENFFSDGPDVKKLCVLSEILKDTSIAINHVIISSHSLENFQHECTSILEKLAMEGQFALARRVAELAELPVDNLVIEEMTQEMQTLKHIDQWSLKQARIDFWKKCHENFKKNSISSKAASSFFSAQAPECPTEEGRSSSEERHLLLTLAAHWLAQEDLVPVAELEELEKQIWLCRITQYTLREKQEQVELGFSQQISTSGELSFDGIASKFSLSMLAVLNTSKYLELSGFPSKETCDSGLDWKEQESLNFLIGHLLDDGFVHEASRVCRYFHFYNQDVALVLHCRALASGEASVDDLHPEIHALLQSAEPLEEEEAGIPLRKVQSSSSLDSQSFVMLPPSDEVVTNLETLTSKCLHGKNYCRQVLCLYELAKELGCSYTDVASQDGEAMLRAILASQQPDRCKRAQAFISTQGLEADTVAELVAEEVTRELLTPPEGTGHQQMFAPAEESRTFLQLTTLCQDRTLVGMKLLDKISSVPHGELSCTTELLILAHHCFTLTCHMEGIIRVLQAARLLTDKHLAPNEEYGLMVRLLTGIGRYNEMTYIFDLLHNKHYFEVLMRKKLDPSGSLKTALLDYIKRCRPGDSEKHNMIALCFSMCREIGENHEAAARIQLKLIESQPWEDSLKDGHHLKQLLLKALTLMLDAAESYAKDSCVRQAQHCHRLTKLITLQIHFLNTGQNTMLINLGRHRLMDCILALPRFYQASIVAEAYDFVPDWAEILYQQVILKGDFNYLEEFKQQRLLRSSIFEEISKKYKQHQPTDTAVKNLKKLLTYCEDVYLYYKLAYEHKFYDIVNVLLKDPQSGCCLKDMLAG

Target ORF information:

RefSeq Version	XM_014109490.2
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris SPG11, spatacsin vesicle trafficking associated (SPG11), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014109490.2

ORF Insert Sequence:

ATGGCCGCAG GAGAGGCAGC TGCGAGAGCT GCTGGTTGCA GCAGCTGCGG CGCTGAAACT 
ATGGAACGGG TTCTACCTAT GCTGTTGGTG CCGGTCCCTG TGGAGGCAAC CGGGCGGTTG 
GGCTCCCGGG CGCAGTTGCA CAGAGAGCAG GAGGTTCTGG GGAGCCTAAC AGCTGCAGGC 
AGCCTGCAGG TGCTTTCCTT GGCGCCGGGC GGCCGGAGCG GCGGGGCCTG CTGCCTCAAT 
GGCCCCTTTC GGCACTTTAT ATGGGAGGAT TCTCATAACG GCAGCTCACC AACAGACAAG 
CCCAAACTTC TTGCTCTTAG TGAAAACTAT GAACTGTTTA TCTATGAATT TAATTTGAAA 
GATGGAAGAT GTGATGCAAC CATTTTGTAT AGCTGTAGTG AGGAGACATT GCAGAAGCTC 
ATTGAAGATC AAAATATCAG TGTTTCCTTT TTATCTTTGA GAATTCTGTC ATTTCACAAT 
AACACATCAT TATTGTTCAT CAATAAATGT ATTGTCCTAC ACATTATATT TCCTGAAAGA 
GATGCTGAGA TCAGAGTACT CAACTGTTTC ACGTTACCCT TGCCTGCACA GGCAGTGGAC 
AGGATCATTG ATGTGCAGCT CTGCAGAGGA ATTCTTTTTG TTTTGAGTAG TTTAGGCTGG 
ATCTACATTT TTGACATTGT GGATGGAAGA CATGTAGCTC ACGTGGATTT AGCACTTCAC 
CAAGAAGATA TGTGTAATGA ACAGCAACAG GAACTAGCCA AGATTTCTTC CTTTACTTCA 
CTGAAAGTGT CTCAAGACCT AGATGTTGTA GTGATTGTCA GCTCCTTCAA CTCTGCAGTT 
GCTCTTAACC TAAACTTGTA TTTCAGGCAA CACCCAGGAC ACCTACTGTG TGAAAGAACT 
CTAGAAGACA TTCCTATTGA AGGCCCTGAG GGGATCGATG AAGATGATCC TGTTAATTCT 
GACTACAACA TGAAACTGAC CAAGTTTTCC TTTCAAGTTG ATAGGTCTTG GAAAGCCCAG 
CTATCATCAC TGAATGAAAT AATAAAGAGC TCCAAATGGG AGGTTCCCTG TTGTGCTCCA 
TGGTTCCAGA ATATTTTGCA TTTGGAGTCA CCGGAATCTG GTTACCACAG TACTAGTACA 
CCAGGCTGGG CCTTCATCCC ACAGGATGAA ATTCATAGCC ACTACAAGTT TCCACAGAAA 
GATCATGCCA ACACCGGTGA CCCAGGAAGA TCATGGAAAA TAATGAACAT TAGTGAACTA 
AAGGAACCTG TTGAGCTTAC ATGTATGTCT GTGACAGGAT TCAGTGTACT GTTTACTTGG 
GCAGTGGAAA AGATGGGCTG CACCATTGTC CTTTGGGATT TGGAGACCCA GGGTATCCAG 
TGTTTTTCCC TTGGCAAAAG GTGTATTCCT ATAGATATGA GTGGAGACCA GCAGCTGTGC 
CTTGTTTTGA CAGAAAATGG ACTCTCCCTG ATTTTGTTTG GATTGACTCA GGAGGAGTTT 
TTAAATAGAC TAATGATCCA TGGAAGTGCC AGCACTGTGG ACTCTCTTTG CCATCTCAAT 
GGTTGGGGGA GGTGCTCAAT CCCCATACAT GCGCTGGAGG CTGGGATAGA AAATCGTCAG 
CTGGACACCG TAGATTTCTT TTTGAAGAGC AAAGAAAATC TTTTGAATCC ATCCTCAAAA 
TCTTCCGTAC CGGATCAGTT TGATGACTTT CCATCTCATT TATATTTAAG AAATGTGGAA 
AAGCTGACAC CAGCATTGGA TTTACTTTGC TCAGCAATTA GAGAAAGTGA TTCTGAAACC 
CAAAGCAAAC ACTTTTCAGA ACAGTTGCTT AACCTTACAC TGTCTTTTCT TAACAAACAA 
ATAAAGGAGC TTTTTGTTCA CAATGAAGAA TTAGATGAAC ATCTACAGAA AGGAGTGAAC 
ATTTTGACCA GCTACATTAA TGAACTTCGA ACCTTCATGA TAAAATTTCC TTGGAAGATA 
GTAGACCCAG TAGATGAATA TGATGTAAGT GAAAATGTCC TCAAAGTAAG AGAGAGTGAT 
ACATGGGAGA AACTCAGCAT TGAGGAAGTT ATTGCCAATG CTATTTTAAA CAACAAAATA 
CCAGAGGCAC AGACTTTCTT CAGGATTAAC AATCATTCTG CTCAAAGACT TGAGGAGCTG 
ATTAGAATAG GCCTAAATTT GGTCTTTGAC TGTTTGAAAA AGAACAATAT AAAGGAAGCA 
TCTGAACTTT TGAAGAATAT GGGCTTTGAT GTAAAAGACC AGTTGCTCAA GATATGTTTC 
TATACAACTG ATAAAAATAT ACGGGACTTT TTGGTGGAAA TTTTAAAAGA AAAAAATTAT 
TTTTCTGAAA ATGAAAAAAG AACTATAGAC TTCGTGCATC AAGTTGAGAA GTTTTATTCA 
GGACATTTTC AAGAAAATGT GCAGATCCAG TCCTTTACCA GGTATTGGAT AAAGGAACAG 
GATTTTTTCA AGCACAAATC TGTTTTGGAC TCACTCCTGA AATATGATCC TAAAGATAAA 
TTTAACAAAC AGGACCATAG AATTGTGTTA AATTGGGCTC AGTGGTGGAA TCAACTAACA 
CAAGAATCCA TCCTTCTCCC CAGGATAAGT CCAGAAGAAT ACAAATCATG TTCTCCTGAA 
GCCCTTTGGA GCTATCTCAC AGCCCATCAT GATTGGTCAA GCATTAGCTT GTGGATTGAA 
GAATTTCAGG CCCAGAACAG TTGTGTTTTA CTGCAGCAGA ACAAATGGCC CCTTCTGACT 
GTTGATGTTA TAGACCAGAA TACTTGCTGC AACAACTACA TGAGGAATGA AATTTTAGAT 
CAGCTGGCCA GACTTAGTCC TGCAAATTGT CCCTTTTTGG AAAAAAAAGA ATTACATGAA 
ACTCACCCTT GGTTTGAATT TTTGGTTCAG TGTCGACAGG TTTCCAGTAA TTTAACAGAT 
CCCAAACTGA TCTTCCAGGC TAGCCTTGCA AACGCTCAGA TACTGATTCC CAGCAACCAG 
GCCAGTGTAA GCAGTATGCT ATTGGAAGGA CATACTCTCC TGGCCCTTGC TACCACAATG 
TATGCCCCTG GCGGTGTCAG CCAGGTTGTT CAGGATGATG AAAATGAGAC CTGTTTGAAG 
AAAGTAGATC CCCAGTTACT GAAGATGGCA TTAACTCCTT ATCCCAAGCT AAAAACTGCT 
CTCTTCCCAC AGTACACTAC CTCTAATGTT CTGCCGCCTG ATATTACGCT TTACCACCTT 
ATTCAGTCAC TATTGCCCTT TGACTCTAGC AGATTGTTTG GCTGGCAGTC TGCCAACACA 
CTGGCTATTG GAGATGCAGT GAGTCATCTC CCACATTTTT CTAGCCCTGA TCTGGTTAAT 
AAATATGCTA TCATGGAACG TCTGAATTTT GCATATTATT TACAACATGG ACGGCCGTCA 
TTTGCATTTG GTACTTTTCT GGTCCAGGAA TTAACCAAGA GCAAGACTCC CAAGCAGCTG 
ATCCAGCAAG TAGGCAATGA AGCTTATGTT TTAGGGCTCT CCTCCTTCTA CATTCCATCA 
GTAGGAGCTG CCTGTGTTTG TTTCTTAGAA CTGCTTGGCC TCGATAGCCT CAAGCTCAGA 
GTTGACATGA AAGTGGCCAA TATAATTTTG AACTACAAAT GTAGAAATGA AGATGTTCAG 
TACAGCTGTA TCAGAGAGGC TCTAGTTGAA AAACTATCTA AACTGGCTGT GGGTGAAAAG 
GCCACCGTAG AGGAATTGCT TATTCTCTTA GAAGAAGGTA TATGGAACAA CATTCAGCAG 
CAAGAGATAA AGAGGTTATC TAGTGAATCT AGCAGCCAGT GGGCATTAGC AGTACAGTTC 
TGCAGGCTTC ACAATGTGAA GCTGAGCACG TCTTACCTTA GAGAATGTGC CAAAGCAAAT 
GACTGGTTAC AGTTCATTAT TCACAGCCAA CTCTATAACT ATCACCCAGC GGAGGTGAAA 
TCTCTTCTTC AGTATTTCAG TCCTGTCCTT CAAGACCACT TAAGACTAGC TTTTGAGAAC 
CTGACCTCAG TGTCTAAATC CAAAATGGAT AGTGGTGAAG TCTGCAGCAG GTCTCCCCAG 
GAACTCCAGA GAAACAAAGA AGAAATGGCT GATTTCTTTC AAGTTCTACT CCAGTGCTCA 
GAGGAACCAA ACCCCTGGTG CTGGCTCCTA GCTGAAGCAA TGAAACAACA GGCTCCTATC 
CTTAGTGTCC TGGCCTCATG TCTTCAGGAT GCCAGTACTA TTCCTTGTCT CTGTGTTTGG 
ATCATCACAT CTGTGGAGGA CAGTGTTGCA GCTGAAGCAA CAGAACACAT TCAGGGCTCA 
GTGGAAGGCC ACATATGGGA CCTTCAAGAC CTCTCAGTCA TCTGGAGAAC ATTATTAACA 
AGGCAGAAGA ACAAAACTCT CATCAGAGGT TTCCAACTTT TCTTTAAGGA TTCCCCACTA 
CTGTTGATGA TGGAGATGTA TGAACTGTGT ATGTTCTTCA AGAATTATAA AGAAGCTGAA 
GCTAAACTTC TGGAGTTCCA AAAGAGCTTT GAAACTGTTG AAACAGCAGC CACAAGGGTC 
CACACTACCA TCCCTGCCCC ATGGCTGAGG GAGCAGGTGT GTTTTCTTCT GAAGCTTATG 
CTGCAGCAGT GTGGGACCCA GTATGAACTG GGGAAGCTTT TACAGCTATT TGTTGGAATA 
GAAAATTTCT TCTCTGATGG TCCAGATGTG AAGAAGCTCT GTGTCCTTAG TGAAATTTTG 
AAGGACACAT CCATAGCCAT TAATCATGTA ATTATTAGCA GCCACAGCCT TGAGAATTTT 
CAGCATGAAT GTACATCTAT TTTGGAAAAA CTGGCGATGG AGGGACAGTT TGCTTTGGCC 
AGGAGAGTAG CAGAATTAGC TGAATTACCT GTGGACAACT TGGTTATTGA AGAGATGACA 
CAGGAAATGC AAACCTTAAA ACACATTGAC CAGTGGTCCC TGAAACAAGC AAGAATTGAC 
TTCTGGAAAA AATGTCATGA GAATTTTAAG AAAAATTCCA TTTCAAGCAA AGCAGCTTCT 
TCCTTTTTCT CAGCCCAGGC ACCTGAGTGC CCAACTGAAG AGGGTCGGAG CAGCAGTGAG 
GAGCGCCATC TGCTGCTCAC ATTGGCAGCA CACTGGCTTG CTCAGGAGGA TCTGGTGCCG 
GTGGCCGAAC TGGAGGAGCT AGAGAAGCAG ATCTGGCTCT GCCGCATTAC CCAATACACC 
CTCAGAGAAA AACAGGAGCA AGTAGAGCTC GGGTTTTCTC AACAGATTTC AACTAGTGGG 
GAACTTTCCT TTGATGGCAT AGCCAGTAAG TTTTCACTCT CCATGTTGGC TGTTCTGAAC 
ACATCAAAGT ACTTAGAACT CAGTGGCTTT CCATCCAAAG AGACATGTGA TAGCGGACTG 
GATTGGAAGG AGCAGGAGTC ACTGAACTTT CTGATTGGGC ACCTATTGGA TGATGGCTTT 
GTGCATGAAG CAAGTAGAGT GTGTCGGTAT TTTCATTTCT ATAACCAAGA TGTTGCCTTG 
GTGTTGCACT GCAGAGCACT GGCCTCAGGG GAAGCTAGTG TGGATGACCT GCACCCAGAG 
ATCCATGCTC TCCTACAAAG TGCTGAGCCG CTTGAGGAGG AGGAAGCTGG CATTCCACTA 
AGAAAAGTCC AGAGCAGTTC AAGTTTGGAT AGTCAGTCAT TTGTGATGCT GCCCCCCAGT 
GATGAAGTGG TGACTAATCT GGAAACTCTG ACAAGTAAAT GCCTCCATGG GAAGAACTAC 
TGTCGGCAGG TCCTGTGTCT GTATGAACTT GCCAAGGAGT TGGGCTGTTC CTACACAGAC 
GTGGCCTCTC AGGACGGGGA GGCCATGCTC CGGGCAATCT TGGCTTCCCA GCAGCCCGAC 
CGATGCAAGC GAGCCCAGGC TTTCATTAGC ACCCAGGGCC TCGAGGCAGA CACGGTGGCT 
GAACTTGTGG CTGAAGAGGT GACCCGGGAG CTGCTGACCC CACCGGAGGG AACAGGACAT 
CAGCAGATGT TTGCCCCAGC AGAGGAAAGC CGGACATTTC TTCAGCTGAC CACTCTGTGT 
CAAGACCGCA CACTGGTAGG CATGAAGTTG TTGGACAAGA TTTCGTCCGT TCCCCATGGA 
GAATTATCTT GCACCACAGA GCTGCTGATC CTGGCCCATC ACTGCTTCAC ACTGACATGC 
CACATGGAGG GCATCATCCG AGTCCTGCAA GCTGCCCGGC TGCTCACGGA CAAACACTTG 
GCCCCCAATG AAGAGTACGG GCTGATGGTA CGCCTCCTCA CCGGCATCGG AAGGTACAAC 
GAGATGACGT ACATATTTGA CTTGCTGCAT AATAAGCACT ATTTTGAAGT GCTGATGAGG 
AAGAAGTTGG ATCCGAGTGG CTCCCTGAAG ACCGCCCTAC TGGACTACAT CAAACGCTGC 
CGCCCTGGGG ACAGTGAGAA GCACAACATG ATCGCTCTGT GCTTCAGCAT GTGTCGGGAG 
ATTGGGGAGA ACCATGAGGC AGCTGCCCGC ATCCAGCTGA AACTGATCGA GTCTCAGCCC 
TGGGAGGACA GCCTCAAGGA CGGGCACCAC CTGAAGCAGC TGCTGCTGAA AGCCCTGACT 
CTGATGCTGG ATGCAGCCGA GAGCTATGCC AAGGACTCCT GTGTGCGACA GGCCCAGCAC 
TGTCACCGGC TCACCAAGTT GATAACACTG CAGATTCATT TTCTGAACAC TGGCCAGAAC 
ACAATGCTCA TCAACCTGGG CCGCCACCGG CTGATGGACT GTATCCTCGC TCTACCTCGG 
TTCTACCAGG CTTCTATTGT GGCCGAAGCC TATGACTTTG TTCCTGATTG GGCAGAAATC 
CTCTACCAAC AAGTGATTCT TAAAGGAGAC TTTAATTACC TGGAAGAATT TAAGCAGCAA 
AGGTTATTAA GATCCAGTAT ATTTGAAGAG ATTTCAAAAA AATATAAACA ACATCAGCCG 
ACAGACACGG CTGTGAAAAA CCTGAAGAAG TTACTCACAT ACTGTGAAGA TGTCTACTTG 
TACTACAAGC TCGCATATGA ACACAAATTT TATGACATTG TGAATGTGCT TCTGAAGGAC 
CCTCAGAGCG GCTGCTGTCT AAAGGACATG CTAGCAGGTT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SPG11 ( XM_014109491.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_013964966.1 Canis lupus familiaris spastic paraplegia 11 (autosomal recessive) (SPG11), transcript variant X3, mRNA.

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CloneID	OCe44279
Clone ID Related Accession (Same CDS sequence)	XM_014109491.1 , XM_014109491.2
Accession Version	XM_014109491.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7101bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1442332800000
Organism	Canis lupus familiaris(Dog)
Product	spatacsin isoform X3
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726113.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCGCAG GAGAGGCAGC TGCGAGAGCT GCTGGTTGCA GCAGCTGCGG CGCTGAAACT 
ATGGAACGGG TTCTACCTAT GCTGTTGGTG CCGGTCCCTG TGGAGGCAAC CGGGCGGTTG 
GGCTCCCGGG CGCAGTTGCA CAGAGAGCAG GAGGTTCTGG GGAGCCTAAC AGCTGCAGGC 
AGCCTGCAGG TGCTTTCCTT GGCGCCGGGC GGCCGGAGCG GCGGGGCCTG CTGCCTCAAT 
GGCCCCTTTC GGCACTTTAT ATGGGAGGAT TCTCATAACG GCAGCTCACC AACAGACAAG 
CCCAAACTTC TTGCTCTTAG TGAAAACTAT GAACTGTTTA TCTATGAATT TAATTTGAAA 
GATGGAAGAT GTGATGCAAC CATTTTGTAT AGCTGTAGTG AGGAGACATT GCAGAAGCTC 
ATTGAAGATC AAAATATCAA CATTTTTGAC ATTGTGGATG GAAGACATGT AGCTCACGTG 
GATTTAGCAC TTCACCAAGA AGATATGTGT AATGAACAGC AACAGGAACT AGCCAAGATT 
TCTTCCTTTA CTTCACTGAA AGTGTCTCAA GACCTAGATG TTGTAGTGAT TGTCAGCTCC 
TTCAACTCTG CAGTTGCTCT TAACCTAAAC TTGTATTTCA GGCAACACCC AGGACACCTA 
CTGTGTGAAA GAACTCTAGA AGACATTCCT ATTGAAGGCC CTGAGGGGAT CGATGAAGAT 
GATCCTGTTA ATTCTGACTA CAACATGAAA CTGACCAAGT TTTCCTTTCA AGTTGATAGG 
TCTTGGAAAG CCCAGCTATC ATCACTGAAT GAAATAATAA AGAGCTCCAA ATGGGAGGTT 
CCCTGTTGTG CTCCATGGTT CCAGAATATT TTGCATTTGG AGTCACCGGA ATCTGGTTAC 
CACAGTACTA GTACACCAGG CTGGGCCTTC ATCCCACAGG ATGAAATTCA TAGCCACTAC 
AAGTTTCCAC AGAAAGATCA TGCCAACACC GGTGACCCAG GAAGATCATG GAAAATAATG 
AACATTAGTG AACTAAAGGA ACCTGTTGAG CTTACATGTA TGTCTGTGAC AGGATTCAGT 
GTACTGTTTA CTTGGGCAGT GGAAAAGATG GGCTGCACCA TTGTCCTTTG GGATTTGGAG 
ACCCAGGGTA TCCAGTGTTT TTCCCTTGGC AAAAGGTGTA TTCCTATAGA TATGAGTGGA 
GACCAGCAGC TGTGCCTTGT TTTGACAGAA AATGGACTCT CCCTGATTTT GTTTGGATTG 
ACTCAGGAGG AGTTTTTAAA TAGACTAATG ATCCATGGAA GTGCCAGCAC TGTGGACTCT 
CTTTGCCATC TCAATGGTTG GGGGAGGTGC TCAATCCCCA TACATGCGCT GGAGGCTGGG 
ATAGAAAATC GTCAGCTGGA CACCGTAGAT TTCTTTTTGA AGAGCAAAGA AAATCTTTTG 
AATCCATCCT CAAAATCTTC CGTACCGGAT CAGTTTGATG ACTTTCCATC TCATTTATAT 
TTAAGAAATG TGGAAAAGCT GACACCAGCA TTGGATTTAC TTTGCTCAGC AATTAGAGAA 
AGTGATTCTG AAACCCAAAG CAAACACTTT TCAGAACAGT TGCTTAACCT TACACTGTCT 
TTTCTTAACA AACAAATAAA GGAGCTTTTT GTTCACAATG AAGAATTAGA TGAACATCTA 
CAGAAAGGAG TGAACATTTT GACCAGCTAC ATTAATGAAC TTCGAACCTT CATGATAAAA 
TTTCCTTGGA AGATAGTAGA CCCAGTAGAT GAATATGATG TAAGTGAAAA TGTCCTCAAA 
GTAAGAGAGA GTGATACATG GGAGAAACTC AGCATTGAGG AAGTTATTGC CAATGCTATT 
TTAAACAACA AAATACCAGA GGCACAGACT TTCTTCAGGA TTAACAATCA TTCTGCTCAA 
AGACTTGAGG AGCTGATTAG AATAGGCCTA AATTTGGTCT TTGACTGTTT GAAAAAGAAC 
AATATAAAGG AAGCATCTGA ACTTTTGAAG AATATGGGCT TTGATGTAAA AGACCAGTTG 
CTCAAGATAT GTTTCTATAC AACTGATAAA AATATACGGG ACTTTTTGGT GGAAATTTTA 
AAAGAAAAAA ATTATTTTTC TGAAAATGAA AAAAGAACTA TAGACTTCGT GCATCAAGTT 
GAGAAGTTTT ATTCAGGACA TTTTCAAGAA AATGTGCAGA TCCAGTCCTT TACCAGGTAT 
TGGATAAAGG AACAGGATTT TTTCAAGCAC AAATCTGTTT TGGACTCACT CCTGAAATAT 
GATCCTAAAG ATAAATTTAA CAAACAGGAC CATAGAATTG TGTTAAATTG GGCTCAGTGG 
TGGAATCAAC TAACACAAGA ATCCATCCTT CTCCCCAGGA TAAGTCCAGA AGAATACAAA 
TCATGTTCTC CTGAAGCCCT TTGGAGCTAT CTCACAGCCC ATCATGATTG GTCAAGCATT 
AGCTTGTGGA TTGAAGAATT TCAGGCCCAG AACAGTTGTG TTTTACTGCA GCAGAACAAA 
TGGCCCCTTC TGACTGTTGA TGTTATAGAC CAGAATACTT GCTGCAACAA CTACATGAGG 
AATGAAATTT TAGATCAGCT GGCCAGGAAT GGGATTTTTC TTCAATCTGA GCTAGAAGAC 
TTTGAACTCT TCCTGCTAAG ACTGAATCGC ATTGGGGGTG TGATGCAAGA TGCTCTCCCC 
ATTCAAAACT ACAGATCCAA AGAAGGTTGG GATTTCCATT CTCACTTCAT TCTCTATTGT 
TTGGAACATG GTCTGCAGTA TCCTCTCTAT GTCTATCTTG ACTTTTACAA ACTTAGTCCT 
GCAAATTGTC CCTTTTTGGA AAAAAAAGAA TTACATGAAA CTCACCCTTG GTTTGAATTT 
TTGGTTCAGT GTCGACAGGT TTCCAGTAAT TTAACAGATC CCAAACTGAT CTTCCAGGCT 
AGCCTTGCAA ACGCTCAGAT ACTGATTCCC AGCAACCAGG CCAGTGTAAG CAGTATGCTA 
TTGGAAGGAC ATACTCTCCT GGCCCTTGCT ACCACAATGT ATGCCCCTGG CGGTGTCAGC 
CAGGTTGTTC AGGATGATGA AAATGAGACC TGTTTGAAGA AAGTAGATCC CCAGTTACTG 
AAGATGGCAT TAACTCCTTA TCCCAAGCTA AAAACTGCTC TCTTCCCACA GTACACTACC 
TCTAATGTTC TGCCGCCTGA TATTACGCTT TACCACCTTA TTCAGTCACT ATTGCCCTTT 
GACTCTAGCA GATTGTTTGG CTGGCAGTCT GCCAACACAC TGGCTATTGG AGATGCAGTG 
AGTCATCTCC CACATTTTTC TAGCCCTGAT CTGGTTAATA AATATGCTAT CATGGAACGT 
CTGAATTTTG CATATTATTT ACAACATGGA CGGCCGTCAT TTGCATTTGG TACTTTTCTG 
GTCCAGGAAT TAACCAAGAG CAAGACTCCC AAGCAGCTGA TCCAGCAAGT AGGCAATGAA 
GCTTATGTTT TAGGGCTCTC CTCCTTCTAC ATTCCATCAG TAGGAGCTGC CTGTGTTTGT 
TTCTTAGAAC TGCTTGGCCT CGATAGCCTC AAGCTCAGAG TTGACATGAA AGTGGCCAAT 
ATAATTTTGA ACTACAAATG TAGAAATGAA GATGTTCAGT ACAGCTGTAT CAGAGAGGCT 
CTAGTTGAAA AACTATCTAA ACTGGCTGTG GGTGAAAAGG CCACCGTAGA GGAATTGCTT 
ATTCTCTTAG AAGAAGGTAT ATGGAACAAC ATTCAGCAGC AAGAGATAAA GAGGTTATCT 
AGTGAATCTA GCAGCCAGTG GGCATTAGCA GTACAGTTCT GCAGGCTTCA CAATGTGAAG 
CTGAGCACGT CTTACCTTAG AGAATGTGCC AAAGCAAATG ACTGGTTACA GTTCATTATT 
CACAGCCAAC TCTATAACTA TCACCCAGCG GAGGTGAAAT CTCTTCTTCA GTATTTCAGT 
CCTGTCCTTC AAGACCACTT AAGACTAGCT TTTGAGAACC TGACCTCAGT GTCTAAATCC 
AAAATGGATA GTGGTGAAGT CTGCAGCAGG TCTCCCCAGG AACTCCAGAG AAACAAAGAA 
GAAATGGCTG ATTTCTTTCA AGTTCTACTC CAGTGCTCAG AGGAACCAAA CCCCTGGTGC 
TGGCTCCTAG CTGAAGCAAT GAAACAACAG GCTCCTATCC TTAGTGTCCT GGCCTCATGT 
CTTCAGGATG CCAGTACTAT TCCTTGTCTC TGTGTTTGGA TCATCACATC TGTGGAGGAC 
AGTGTTGCAG CTGAAGCAAC AGAACACATT CAGGGCTCAG TGGAAGGCCA CATATGGGAC 
CTTCAAGACC TCTCAGTCAT CTGGAGAACA TTATTAACAA GGCAGAAGAA CAAAACTCTC 
ATCAGAGGTT TCCAACTTTT CTTTAAGGAT TCCCCACTAC TGTTGATGAT GGAGATGTAT 
GAACTGTGTA TGTTCTTCAA GAATTATAAA GAAGCTGAAG CTAAACTTCT GGAGTTCCAA 
AAGAGCTTTG AAACTGTTGA AACAGCAGCC ACAAGGGTCC ACACTACCAT CCCTGCCCCA 
TGGCTGAGGG AGCAGGTGTG TTTTCTTCTG AAGCTTATGC TGCAGCAGTG TGGGACCCAG 
TATGAACTGG GGAAGCTTTT ACAGCTATTT GTTGGAATAG AAAATTTCTT CTCTGATGGT 
CCAGATGTGA AGAAGCTCTG TGTCCTTAGT GAAATTTTGA AGGACACATC CATAGCCATT 
AATCATGTAA TTATTAGCAG CCACAGCCTT GAGAATTTTC AGCATGAATG TACATCTATT 
TTGGAAAAAC TGGCGATGGA GGGACAGTTT GCTTTGGCCA GGAGAGTAGC AGAATTAGCT 
GAATTACCTG TGGACAACTT GGTTATTGAA GAGATGACAC AGGAAATGCA AACCTTAAAA 
CACATTGACC AGTGGTCCCT GAAACAAGCA AGAATTGACT TCTGGAAAAA ATGTCATGAG 
AATTTTAAGA AAAATTCCAT TTCAAGCAAA GCAGCTTCTT CCTTTTTCTC AGCCCAGGCA 
CCTGAGTGCC CAACTGAAGA GGGTCGGAGC AGCAGTGAGG AGCGCCATCT GCTGCTCACA 
TTGGCAGCAC ACTGGCTTGC TCAGGAGGAT CTGGTGCCGG TGGCCGAACT GGAGGAGCTA 
GAGAAGCAGA TCTGGCTCTG CCGCATTACC CAATACACCC TCAGAGAAAA ACAGGAGCAA 
GTAGAGCTCG GGTTTTCTCA ACAGATTTCA ACTAGTGGGG AACTTTCCTT TGATGGCATA 
GCCAGTAAGT TTTCACTCTC CATGTTGGCT GTTCTGAACA CATCAAAGTA CTTAGAACTC 
AGTGGCTTTC CATCCAAAGA GACATGTGAT AGCGGACTGG ATTGGAAGGA GCAGGAGTCA 
CTGAACTTTC TGATTGGGCA CCTATTGGAT GATGGCTTTG TGCATGAAGC AAGTAGAGTG 
TGTCGGTATT TTCATTTCTA TAACCAAGAT GTTGCCTTGG TGTTGCACTG CAGAGCACTG 
GCCTCAGGGG AAGCTAGTGT GGATGACCTG CACCCAGAGA TCCATGCTCT CCTACAAAGT 
GCTGAGCCGC TTGAGGAGGA GGAAGCTGGC ATTCCACTAA GAAAAGTCCA GAGCAGTTCA 
AGTTTGGATA GTCAGTCATT TGTGATGCTG CCCCCCAGTG ATGAAGTGGT GACTAATCTG 
GAAACTCTGA CAAGTAAATG CCTCCATGGG AAGAACTACT GTCGGCAGGT CCTGTGTCTG 
TATGAACTTG CCAAGGAGTT GGGCTGTTCC TACACAGACG TGGCCTCTCA GGACGGGGAG 
GCCATGCTCC GGGCAATCTT GGCTTCCCAG CAGCCCGACC GATGCAAGCG AGCCCAGGCT 
TTCATTAGCA CCCAGGGCCT CGAGGCAGAC ACGGTGGCTG AACTTGTGGC TGAAGAGGTG 
ACCCGGGAGC TGCTGACCCC ACCGGAGGGA ACAGGACATC AGCAGATGTT TGCCCCAGCA 
GAGGAAAGCC GGACATTTCT TCAGCTGACC ACTCTGTGTC AAGACCGCAC ACTGGTAGGC 
ATGAAGTTGT TGGACAAGAT TTCGTCCGTT CCCCATGGAG AATTATCTTG CACCACAGAG 
CTGCTGATCC TGGCCCATCA CTGCTTCACA CTGACATGCC ACATGGAGGG CATCATCCGA 
GTCCTGCAAG CTGCCCGGCT GCTCACGGAC AAACACTTGG CCCCCAATGA AGAGTACGGG 
CTGATGGTAC GCCTCCTCAC CGGCATCGGA AGGTACAACG AGATGACGTA CATATTTGAC 
TTGCTGCATA ATAAGCACTA TTTTGAAGTG CTGATGAGGA AGAAGTTGGA TCCGAGTGGC 
TCCCTGAAGA CCGCCCTACT GGACTACATC AAACGCTGCC GCCCTGGGGA CAGTGAGAAG 
CACAACATGA TCGCTCTGTG CTTCAGCATG TGTCGGGAGA TTGGGGAGAA CCATGAGGCA 
GCTGCCCGCA TCCAGCTGAA ACTGATCGAG TCTCAGCCCT GGGAGGACAG CCTCAAGGAC 
GGGCACCACC TGAAGCAGCT GCTGCTGAAA GCCCTGACTC TGATGCTGGA TGCAGCCGAG 
AGCTATGCCA AGGACTCCTG TGTGCGACAG GCCCAGCACT GTCACCGGCT CACCAAGTTG 
ATAACACTGC AGATTCATTT TCTGAACACT GGCCAGAACA CAATGCTCAT CAACCTGGGC 
CGCCACCGGC TGATGGACTG TATCCTCGCT CTACCTCGGT TCTACCAGGC TTCTATTGTG 
GCCGAAGCCT ATGACTTTGT TCCTGATTGG GCAGAAATCC TCTACCAACA AGTGATTCTT 
AAAGGAGACT TTAATTACCT GGAAGAATTT AAGCAGCAAA GGTTATTAAG ATCCAGTATA 
TTTGAAGAGA TTTCAAAAAA ATATAAACAA CATCAGCCGA CAGACACGGC TGTGAAAAAC 
CTGAAGAAGT TACTCACATA CTGTGAAGAT GTCTACTTGT ACTACAAGCT CGCATATGAA 
CACAAATTTT ATGACATTGT GAATGTGCTT CTGAAGGACC CTCAGAGCGG CTGCTGTCTA 
AAGGACATGC TAGCAGGTTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013964966.1
CDS	12..7112
Translation	MAAGEAAARAAGCSSCGAETMERVLPMLLVPVPVEATGRLGSRAQLHREQEVLGSLTAAGSLQVLSLAPGGRSGGACCLNGPFRHFIWEDSHNGSSPTDKPKLLALSENYELFIYEFNLKDGRCDATILYSCSEETLQKLIEDQNINIFDIVDGRHVAHVDLALHQEDMCNEQQQELAKISSFTSLKVSQDLDVVVIVSSFNSAVALNLNLYFRQHPGHLLCERTLEDIPIEGPEGIDEDDPVNSDYNMKLTKFSFQVDRSWKAQLSSLNEIIKSSKWEVPCCAPWFQNILHLESPESGYHSTSTPGWAFIPQDEIHSHYKFPQKDHANTGDPGRSWKIMNISELKEPVELTCMSVTGFSVLFTWAVEKMGCTIVLWDLETQGIQCFSLGKRCIPIDMSGDQQLCLVLTENGLSLILFGLTQEEFLNRLMIHGSASTVDSLCHLNGWGRCSIPIHALEAGIENRQLDTVDFFLKSKENLLNPSSKSSVPDQFDDFPSHLYLRNVEKLTPALDLLCSAIRESDSETQSKHFSEQLLNLTLSFLNKQIKELFVHNEELDEHLQKGVNILTSYINELRTFMIKFPWKIVDPVDEYDVSENVLKVRESDTWEKLSIEEVIANAILNNKIPEAQTFFRINNHSAQRLEELIRIGLNLVFDCLKKNNIKEASELLKNMGFDVKDQLLKICFYTTDKNIRDFLVEILKEKNYFSENEKRTIDFVHQVEKFYSGHFQENVQIQSFTRYWIKEQDFFKHKSVLDSLLKYDPKDKFNKQDHRIVLNWAQWWNQLTQESILLPRISPEEYKSCSPEALWSYLTAHHDWSSISLWIEEFQAQNSCVLLQQNKWPLLTVDVIDQNTCCNNYMRNEILDQLARNGIFLQSELEDFELFLLRLNRIGGVMQDALPIQNYRSKEGWDFHSHFILYCLEHGLQYPLYVYLDFYKLSPANCPFLEKKELHETHPWFEFLVQCRQVSSNLTDPKLIFQASLANAQILIPSNQASVSSMLLEGHTLLALATTMYAPGGVSQVVQDDENETCLKKVDPQLLKMALTPYPKLKTALFPQYTTSNVLPPDITLYHLIQSLLPFDSSRLFGWQSANTLAIGDAVSHLPHFSSPDLVNKYAIMERLNFAYYLQHGRPSFAFGTFLVQELTKSKTPKQLIQQVGNEAYVLGLSSFYIPSVGAACVCFLELLGLDSLKLRVDMKVANIILNYKCRNEDVQYSCIREALVEKLSKLAVGEKATVEELLILLEEGIWNNIQQQEIKRLSSESSSQWALAVQFCRLHNVKLSTSYLRECAKANDWLQFIIHSQLYNYHPAEVKSLLQYFSPVLQDHLRLAFENLTSVSKSKMDSGEVCSRSPQELQRNKEEMADFFQVLLQCSEEPNPWCWLLAEAMKQQAPILSVLASCLQDASTIPCLCVWIITSVEDSVAAEATEHIQGSVEGHIWDLQDLSVIWRTLLTRQKNKTLIRGFQLFFKDSPLLLMMEMYELCMFFKNYKEAEAKLLEFQKSFETVETAATRVHTTIPAPWLREQVCFLLKLMLQQCGTQYELGKLLQLFVGIENFFSDGPDVKKLCVLSEILKDTSIAINHVIISSHSLENFQHECTSILEKLAMEGQFALARRVAELAELPVDNLVIEEMTQEMQTLKHIDQWSLKQARIDFWKKCHENFKKNSISSKAASSFFSAQAPECPTEEGRSSSEERHLLLTLAAHWLAQEDLVPVAELEELEKQIWLCRITQYTLREKQEQVELGFSQQISTSGELSFDGIASKFSLSMLAVLNTSKYLELSGFPSKETCDSGLDWKEQESLNFLIGHLLDDGFVHEASRVCRYFHFYNQDVALVLHCRALASGEASVDDLHPEIHALLQSAEPLEEEEAGIPLRKVQSSSSLDSQSFVMLPPSDEVVTNLETLTSKCLHGKNYCRQVLCLYELAKELGCSYTDVASQDGEAMLRAILASQQPDRCKRAQAFISTQGLEADTVAELVAEEVTRELLTPPEGTGHQQMFAPAEESRTFLQLTTLCQDRTLVGMKLLDKISSVPHGELSCTTELLILAHHCFTLTCHMEGIIRVLQAARLLTDKHLAPNEEYGLMVRLLTGIGRYNEMTYIFDLLHNKHYFEVLMRKKLDPSGSLKTALLDYIKRCRPGDSEKHNMIALCFSMCREIGENHEAAARIQLKLIESQPWEDSLKDGHHLKQLLLKALTLMLDAAESYAKDSCVRQAQHCHRLTKLITLQIHFLNTGQNTMLINLGRHRLMDCILALPRFYQASIVAEAYDFVPDWAEILYQQVILKGDFNYLEEFKQQRLLRSSIFEEISKKYKQHQPTDTAVKNLKKLLTYCEDVYLYYKLAYEHKFYDIVNVLLKDPQSGCCLKDMLAG

Target ORF information:

RefSeq Version	XM_014109491.1
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris spastic paraplegia 11 (autosomal recessive) (SPG11), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014109491.1

ORF Insert Sequence:

ATGGCCGCAG GAGAGGCAGC TGCGAGAGCT GCTGGTTGCA GCAGCTGCGG CGCTGAAACT 
ATGGAACGGG TTCTACCTAT GCTGTTGGTG CCGGTCCCTG TGGAGGCAAC CGGGCGGTTG 
GGCTCCCGGG CGCAGTTGCA CAGAGAGCAG GAGGTTCTGG GGAGCCTAAC AGCTGCAGGC 
AGCCTGCAGG TGCTTTCCTT GGCGCCGGGC GGCCGGAGCG GCGGGGCCTG CTGCCTCAAT 
GGCCCCTTTC GGCACTTTAT ATGGGAGGAT TCTCATAACG GCAGCTCACC AACAGACAAG 
CCCAAACTTC TTGCTCTTAG TGAAAACTAT GAACTGTTTA TCTATGAATT TAATTTGAAA 
GATGGAAGAT GTGATGCAAC CATTTTGTAT AGCTGTAGTG AGGAGACATT GCAGAAGCTC 
ATTGAAGATC AAAATATCAA CATTTTTGAC ATTGTGGATG GAAGACATGT AGCTCACGTG 
GATTTAGCAC TTCACCAAGA AGATATGTGT AATGAACAGC AACAGGAACT AGCCAAGATT 
TCTTCCTTTA CTTCACTGAA AGTGTCTCAA GACCTAGATG TTGTAGTGAT TGTCAGCTCC 
TTCAACTCTG CAGTTGCTCT TAACCTAAAC TTGTATTTCA GGCAACACCC AGGACACCTA 
CTGTGTGAAA GAACTCTAGA AGACATTCCT ATTGAAGGCC CTGAGGGGAT CGATGAAGAT 
GATCCTGTTA ATTCTGACTA CAACATGAAA CTGACCAAGT TTTCCTTTCA AGTTGATAGG 
TCTTGGAAAG CCCAGCTATC ATCACTGAAT GAAATAATAA AGAGCTCCAA ATGGGAGGTT 
CCCTGTTGTG CTCCATGGTT CCAGAATATT TTGCATTTGG AGTCACCGGA ATCTGGTTAC 
CACAGTACTA GTACACCAGG CTGGGCCTTC ATCCCACAGG ATGAAATTCA TAGCCACTAC 
AAGTTTCCAC AGAAAGATCA TGCCAACACC GGTGACCCAG GAAGATCATG GAAAATAATG 
AACATTAGTG AACTAAAGGA ACCTGTTGAG CTTACATGTA TGTCTGTGAC AGGATTCAGT 
GTACTGTTTA CTTGGGCAGT GGAAAAGATG GGCTGCACCA TTGTCCTTTG GGATTTGGAG 
ACCCAGGGTA TCCAGTGTTT TTCCCTTGGC AAAAGGTGTA TTCCTATAGA TATGAGTGGA 
GACCAGCAGC TGTGCCTTGT TTTGACAGAA AATGGACTCT CCCTGATTTT GTTTGGATTG 
ACTCAGGAGG AGTTTTTAAA TAGACTAATG ATCCATGGAA GTGCCAGCAC TGTGGACTCT 
CTTTGCCATC TCAATGGTTG GGGGAGGTGC TCAATCCCCA TACATGCGCT GGAGGCTGGG 
ATAGAAAATC GTCAGCTGGA CACCGTAGAT TTCTTTTTGA AGAGCAAAGA AAATCTTTTG 
AATCCATCCT CAAAATCTTC CGTACCGGAT CAGTTTGATG ACTTTCCATC TCATTTATAT 
TTAAGAAATG TGGAAAAGCT GACACCAGCA TTGGATTTAC TTTGCTCAGC AATTAGAGAA 
AGTGATTCTG AAACCCAAAG CAAACACTTT TCAGAACAGT TGCTTAACCT TACACTGTCT 
TTTCTTAACA AACAAATAAA GGAGCTTTTT GTTCACAATG AAGAATTAGA TGAACATCTA 
CAGAAAGGAG TGAACATTTT GACCAGCTAC ATTAATGAAC TTCGAACCTT CATGATAAAA 
TTTCCTTGGA AGATAGTAGA CCCAGTAGAT GAATATGATG TAAGTGAAAA TGTCCTCAAA 
GTAAGAGAGA GTGATACATG GGAGAAACTC AGCATTGAGG AAGTTATTGC CAATGCTATT 
TTAAACAACA AAATACCAGA GGCACAGACT TTCTTCAGGA TTAACAATCA TTCTGCTCAA 
AGACTTGAGG AGCTGATTAG AATAGGCCTA AATTTGGTCT TTGACTGTTT GAAAAAGAAC 
AATATAAAGG AAGCATCTGA ACTTTTGAAG AATATGGGCT TTGATGTAAA AGACCAGTTG 
CTCAAGATAT GTTTCTATAC AACTGATAAA AATATACGGG ACTTTTTGGT GGAAATTTTA 
AAAGAAAAAA ATTATTTTTC TGAAAATGAA AAAAGAACTA TAGACTTCGT GCATCAAGTT 
GAGAAGTTTT ATTCAGGACA TTTTCAAGAA AATGTGCAGA TCCAGTCCTT TACCAGGTAT 
TGGATAAAGG AACAGGATTT TTTCAAGCAC AAATCTGTTT TGGACTCACT CCTGAAATAT 
GATCCTAAAG ATAAATTTAA CAAACAGGAC CATAGAATTG TGTTAAATTG GGCTCAGTGG 
TGGAATCAAC TAACACAAGA ATCCATCCTT CTCCCCAGGA TAAGTCCAGA AGAATACAAA 
TCATGTTCTC CTGAAGCCCT TTGGAGCTAT CTCACAGCCC ATCATGATTG GTCAAGCATT 
AGCTTGTGGA TTGAAGAATT TCAGGCCCAG AACAGTTGTG TTTTACTGCA GCAGAACAAA 
TGGCCCCTTC TGACTGTTGA TGTTATAGAC CAGAATACTT GCTGCAACAA CTACATGAGG 
AATGAAATTT TAGATCAGCT GGCCAGGAAT GGGATTTTTC TTCAATCTGA GCTAGAAGAC 
TTTGAACTCT TCCTGCTAAG ACTGAATCGC ATTGGGGGTG TGATGCAAGA TGCTCTCCCC 
ATTCAAAACT ACAGATCCAA AGAAGGTTGG GATTTCCATT CTCACTTCAT TCTCTATTGT 
TTGGAACATG GTCTGCAGTA TCCTCTCTAT GTCTATCTTG ACTTTTACAA ACTTAGTCCT 
GCAAATTGTC CCTTTTTGGA AAAAAAAGAA TTACATGAAA CTCACCCTTG GTTTGAATTT 
TTGGTTCAGT GTCGACAGGT TTCCAGTAAT TTAACAGATC CCAAACTGAT CTTCCAGGCT 
AGCCTTGCAA ACGCTCAGAT ACTGATTCCC AGCAACCAGG CCAGTGTAAG CAGTATGCTA 
TTGGAAGGAC ATACTCTCCT GGCCCTTGCT ACCACAATGT ATGCCCCTGG CGGTGTCAGC 
CAGGTTGTTC AGGATGATGA AAATGAGACC TGTTTGAAGA AAGTAGATCC CCAGTTACTG 
AAGATGGCAT TAACTCCTTA TCCCAAGCTA AAAACTGCTC TCTTCCCACA GTACACTACC 
TCTAATGTTC TGCCGCCTGA TATTACGCTT TACCACCTTA TTCAGTCACT ATTGCCCTTT 
GACTCTAGCA GATTGTTTGG CTGGCAGTCT GCCAACACAC TGGCTATTGG AGATGCAGTG 
AGTCATCTCC CACATTTTTC TAGCCCTGAT CTGGTTAATA AATATGCTAT CATGGAACGT 
CTGAATTTTG CATATTATTT ACAACATGGA CGGCCGTCAT TTGCATTTGG TACTTTTCTG 
GTCCAGGAAT TAACCAAGAG CAAGACTCCC AAGCAGCTGA TCCAGCAAGT AGGCAATGAA 
GCTTATGTTT TAGGGCTCTC CTCCTTCTAC ATTCCATCAG TAGGAGCTGC CTGTGTTTGT 
TTCTTAGAAC TGCTTGGCCT CGATAGCCTC AAGCTCAGAG TTGACATGAA AGTGGCCAAT 
ATAATTTTGA ACTACAAATG TAGAAATGAA GATGTTCAGT ACAGCTGTAT CAGAGAGGCT 
CTAGTTGAAA AACTATCTAA ACTGGCTGTG GGTGAAAAGG CCACCGTAGA GGAATTGCTT 
ATTCTCTTAG AAGAAGGTAT ATGGAACAAC ATTCAGCAGC AAGAGATAAA GAGGTTATCT 
AGTGAATCTA GCAGCCAGTG GGCATTAGCA GTACAGTTCT GCAGGCTTCA CAATGTGAAG 
CTGAGCACGT CTTACCTTAG AGAATGTGCC AAAGCAAATG ACTGGTTACA GTTCATTATT 
CACAGCCAAC TCTATAACTA TCACCCAGCG GAGGTGAAAT CTCTTCTTCA GTATTTCAGT 
CCTGTCCTTC AAGACCACTT AAGACTAGCT TTTGAGAACC TGACCTCAGT GTCTAAATCC 
AAAATGGATA GTGGTGAAGT CTGCAGCAGG TCTCCCCAGG AACTCCAGAG AAACAAAGAA 
GAAATGGCTG ATTTCTTTCA AGTTCTACTC CAGTGCTCAG AGGAACCAAA CCCCTGGTGC 
TGGCTCCTAG CTGAAGCAAT GAAACAACAG GCTCCTATCC TTAGTGTCCT GGCCTCATGT 
CTTCAGGATG CCAGTACTAT TCCTTGTCTC TGTGTTTGGA TCATCACATC TGTGGAGGAC 
AGTGTTGCAG CTGAAGCAAC AGAACACATT CAGGGCTCAG TGGAAGGCCA CATATGGGAC 
CTTCAAGACC TCTCAGTCAT CTGGAGAACA TTATTAACAA GGCAGAAGAA CAAAACTCTC 
ATCAGAGGTT TCCAACTTTT CTTTAAGGAT TCCCCACTAC TGTTGATGAT GGAGATGTAT 
GAACTGTGTA TGTTCTTCAA GAATTATAAA GAAGCTGAAG CTAAACTTCT GGAGTTCCAA 
AAGAGCTTTG AAACTGTTGA AACAGCAGCC ACAAGGGTCC ACACTACCAT CCCTGCCCCA 
TGGCTGAGGG AGCAGGTGTG TTTTCTTCTG AAGCTTATGC TGCAGCAGTG TGGGACCCAG 
TATGAACTGG GGAAGCTTTT ACAGCTATTT GTTGGAATAG AAAATTTCTT CTCTGATGGT 
CCAGATGTGA AGAAGCTCTG TGTCCTTAGT GAAATTTTGA AGGACACATC CATAGCCATT 
AATCATGTAA TTATTAGCAG CCACAGCCTT GAGAATTTTC AGCATGAATG TACATCTATT 
TTGGAAAAAC TGGCGATGGA GGGACAGTTT GCTTTGGCCA GGAGAGTAGC AGAATTAGCT 
GAATTACCTG TGGACAACTT GGTTATTGAA GAGATGACAC AGGAAATGCA AACCTTAAAA 
CACATTGACC AGTGGTCCCT GAAACAAGCA AGAATTGACT TCTGGAAAAA ATGTCATGAG 
AATTTTAAGA AAAATTCCAT TTCAAGCAAA GCAGCTTCTT CCTTTTTCTC AGCCCAGGCA 
CCTGAGTGCC CAACTGAAGA GGGTCGGAGC AGCAGTGAGG AGCGCCATCT GCTGCTCACA 
TTGGCAGCAC ACTGGCTTGC TCAGGAGGAT CTGGTGCCGG TGGCCGAACT GGAGGAGCTA 
GAGAAGCAGA TCTGGCTCTG CCGCATTACC CAATACACCC TCAGAGAAAA ACAGGAGCAA 
GTAGAGCTCG GGTTTTCTCA ACAGATTTCA ACTAGTGGGG AACTTTCCTT TGATGGCATA 
GCCAGTAAGT TTTCACTCTC CATGTTGGCT GTTCTGAACA CATCAAAGTA CTTAGAACTC 
AGTGGCTTTC CATCCAAAGA GACATGTGAT AGCGGACTGG ATTGGAAGGA GCAGGAGTCA 
CTGAACTTTC TGATTGGGCA CCTATTGGAT GATGGCTTTG TGCATGAAGC AAGTAGAGTG 
TGTCGGTATT TTCATTTCTA TAACCAAGAT GTTGCCTTGG TGTTGCACTG CAGAGCACTG 
GCCTCAGGGG AAGCTAGTGT GGATGACCTG CACCCAGAGA TCCATGCTCT CCTACAAAGT 
GCTGAGCCGC TTGAGGAGGA GGAAGCTGGC ATTCCACTAA GAAAAGTCCA GAGCAGTTCA 
AGTTTGGATA GTCAGTCATT TGTGATGCTG CCCCCCAGTG ATGAAGTGGT GACTAATCTG 
GAAACTCTGA CAAGTAAATG CCTCCATGGG AAGAACTACT GTCGGCAGGT CCTGTGTCTG 
TATGAACTTG CCAAGGAGTT GGGCTGTTCC TACACAGACG TGGCCTCTCA GGACGGGGAG 
GCCATGCTCC GGGCAATCTT GGCTTCCCAG CAGCCCGACC GATGCAAGCG AGCCCAGGCT 
TTCATTAGCA CCCAGGGCCT CGAGGCAGAC ACGGTGGCTG AACTTGTGGC TGAAGAGGTG 
ACCCGGGAGC TGCTGACCCC ACCGGAGGGA ACAGGACATC AGCAGATGTT TGCCCCAGCA 
GAGGAAAGCC GGACATTTCT TCAGCTGACC ACTCTGTGTC AAGACCGCAC ACTGGTAGGC 
ATGAAGTTGT TGGACAAGAT TTCGTCCGTT CCCCATGGAG AATTATCTTG CACCACAGAG 
CTGCTGATCC TGGCCCATCA CTGCTTCACA CTGACATGCC ACATGGAGGG CATCATCCGA 
GTCCTGCAAG CTGCCCGGCT GCTCACGGAC AAACACTTGG CCCCCAATGA AGAGTACGGG 
CTGATGGTAC GCCTCCTCAC CGGCATCGGA AGGTACAACG AGATGACGTA CATATTTGAC 
TTGCTGCATA ATAAGCACTA TTTTGAAGTG CTGATGAGGA AGAAGTTGGA TCCGAGTGGC 
TCCCTGAAGA CCGCCCTACT GGACTACATC AAACGCTGCC GCCCTGGGGA CAGTGAGAAG 
CACAACATGA TCGCTCTGTG CTTCAGCATG TGTCGGGAGA TTGGGGAGAA CCATGAGGCA 
GCTGCCCGCA TCCAGCTGAA ACTGATCGAG TCTCAGCCCT GGGAGGACAG CCTCAAGGAC 
GGGCACCACC TGAAGCAGCT GCTGCTGAAA GCCCTGACTC TGATGCTGGA TGCAGCCGAG 
AGCTATGCCA AGGACTCCTG TGTGCGACAG GCCCAGCACT GTCACCGGCT CACCAAGTTG 
ATAACACTGC AGATTCATTT TCTGAACACT GGCCAGAACA CAATGCTCAT CAACCTGGGC 
CGCCACCGGC TGATGGACTG TATCCTCGCT CTACCTCGGT TCTACCAGGC TTCTATTGTG 
GCCGAAGCCT ATGACTTTGT TCCTGATTGG GCAGAAATCC TCTACCAACA AGTGATTCTT 
AAAGGAGACT TTAATTACCT GGAAGAATTT AAGCAGCAAA GGTTATTAAG ATCCAGTATA 
TTTGAAGAGA TTTCAAAAAA ATATAAACAA CATCAGCCGA CAGACACGGC TGTGAAAAAC 
CTGAAGAAGT TACTCACATA CTGTGAAGAT GTCTACTTGT ACTACAAGCT CGCATATGAA 
CACAAATTTT ATGACATTGT GAATGTGCTT CTGAAGGACC CTCAGAGCGG CTGCTGTCTA 
AAGGACATGC TAGCAGGTTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SPG11 ( XM_014109491.2 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_013964966.1 Canis lupus familiaris SPG11, spatacsin vesicle trafficking associated (SPG11), transcript variant X3, mRNA.

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CloneID	OCe44279
Clone ID Related Accession (Same CDS sequence)	XM_014109491.1 , XM_014109491.2
Accession Version	XM_014109491.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7101bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1504454400000
Organism	Canis lupus familiaris(dog)
Product	spatacsin isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006612.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_014109491.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCGCAG GAGAGGCAGC TGCGAGAGCT GCTGGTTGCA GCAGCTGCGG CGCTGAAACT 
ATGGAACGGG TTCTACCTAT GCTGTTGGTG CCGGTCCCTG TGGAGGCAAC CGGGCGGTTG 
GGCTCCCGGG CGCAGTTGCA CAGAGAGCAG GAGGTTCTGG GGAGCCTAAC AGCTGCAGGC 
AGCCTGCAGG TGCTTTCCTT GGCGCCGGGC GGCCGGAGCG GCGGGGCCTG CTGCCTCAAT 
GGCCCCTTTC GGCACTTTAT ATGGGAGGAT TCTCATAACG GCAGCTCACC AACAGACAAG 
CCCAAACTTC TTGCTCTTAG TGAAAACTAT GAACTGTTTA TCTATGAATT TAATTTGAAA 
GATGGAAGAT GTGATGCAAC CATTTTGTAT AGCTGTAGTG AGGAGACATT GCAGAAGCTC 
ATTGAAGATC AAAATATCAA CATTTTTGAC ATTGTGGATG GAAGACATGT AGCTCACGTG 
GATTTAGCAC TTCACCAAGA AGATATGTGT AATGAACAGC AACAGGAACT AGCCAAGATT 
TCTTCCTTTA CTTCACTGAA AGTGTCTCAA GACCTAGATG TTGTAGTGAT TGTCAGCTCC 
TTCAACTCTG CAGTTGCTCT TAACCTAAAC TTGTATTTCA GGCAACACCC AGGACACCTA 
CTGTGTGAAA GAACTCTAGA AGACATTCCT ATTGAAGGCC CTGAGGGGAT CGATGAAGAT 
GATCCTGTTA ATTCTGACTA CAACATGAAA CTGACCAAGT TTTCCTTTCA AGTTGATAGG 
TCTTGGAAAG CCCAGCTATC ATCACTGAAT GAAATAATAA AGAGCTCCAA ATGGGAGGTT 
CCCTGTTGTG CTCCATGGTT CCAGAATATT TTGCATTTGG AGTCACCGGA ATCTGGTTAC 
CACAGTACTA GTACACCAGG CTGGGCCTTC ATCCCACAGG ATGAAATTCA TAGCCACTAC 
AAGTTTCCAC AGAAAGATCA TGCCAACACC GGTGACCCAG GAAGATCATG GAAAATAATG 
AACATTAGTG AACTAAAGGA ACCTGTTGAG CTTACATGTA TGTCTGTGAC AGGATTCAGT 
GTACTGTTTA CTTGGGCAGT GGAAAAGATG GGCTGCACCA TTGTCCTTTG GGATTTGGAG 
ACCCAGGGTA TCCAGTGTTT TTCCCTTGGC AAAAGGTGTA TTCCTATAGA TATGAGTGGA 
GACCAGCAGC TGTGCCTTGT TTTGACAGAA AATGGACTCT CCCTGATTTT GTTTGGATTG 
ACTCAGGAGG AGTTTTTAAA TAGACTAATG ATCCATGGAA GTGCCAGCAC TGTGGACTCT 
CTTTGCCATC TCAATGGTTG GGGGAGGTGC TCAATCCCCA TACATGCGCT GGAGGCTGGG 
ATAGAAAATC GTCAGCTGGA CACCGTAGAT TTCTTTTTGA AGAGCAAAGA AAATCTTTTG 
AATCCATCCT CAAAATCTTC CGTACCGGAT CAGTTTGATG ACTTTCCATC TCATTTATAT 
TTAAGAAATG TGGAAAAGCT GACACCAGCA TTGGATTTAC TTTGCTCAGC AATTAGAGAA 
AGTGATTCTG AAACCCAAAG CAAACACTTT TCAGAACAGT TGCTTAACCT TACACTGTCT 
TTTCTTAACA AACAAATAAA GGAGCTTTTT GTTCACAATG AAGAATTAGA TGAACATCTA 
CAGAAAGGAG TGAACATTTT GACCAGCTAC ATTAATGAAC TTCGAACCTT CATGATAAAA 
TTTCCTTGGA AGATAGTAGA CCCAGTAGAT GAATATGATG TAAGTGAAAA TGTCCTCAAA 
GTAAGAGAGA GTGATACATG GGAGAAACTC AGCATTGAGG AAGTTATTGC CAATGCTATT 
TTAAACAACA AAATACCAGA GGCACAGACT TTCTTCAGGA TTAACAATCA TTCTGCTCAA 
AGACTTGAGG AGCTGATTAG AATAGGCCTA AATTTGGTCT TTGACTGTTT GAAAAAGAAC 
AATATAAAGG AAGCATCTGA ACTTTTGAAG AATATGGGCT TTGATGTAAA AGACCAGTTG 
CTCAAGATAT GTTTCTATAC AACTGATAAA AATATACGGG ACTTTTTGGT GGAAATTTTA 
AAAGAAAAAA ATTATTTTTC TGAAAATGAA AAAAGAACTA TAGACTTCGT GCATCAAGTT 
GAGAAGTTTT ATTCAGGACA TTTTCAAGAA AATGTGCAGA TCCAGTCCTT TACCAGGTAT 
TGGATAAAGG AACAGGATTT TTTCAAGCAC AAATCTGTTT TGGACTCACT CCTGAAATAT 
GATCCTAAAG ATAAATTTAA CAAACAGGAC CATAGAATTG TGTTAAATTG GGCTCAGTGG 
TGGAATCAAC TAACACAAGA ATCCATCCTT CTCCCCAGGA TAAGTCCAGA AGAATACAAA 
TCATGTTCTC CTGAAGCCCT TTGGAGCTAT CTCACAGCCC ATCATGATTG GTCAAGCATT 
AGCTTGTGGA TTGAAGAATT TCAGGCCCAG AACAGTTGTG TTTTACTGCA GCAGAACAAA 
TGGCCCCTTC TGACTGTTGA TGTTATAGAC CAGAATACTT GCTGCAACAA CTACATGAGG 
AATGAAATTT TAGATCAGCT GGCCAGGAAT GGGATTTTTC TTCAATCTGA GCTAGAAGAC 
TTTGAACTCT TCCTGCTAAG ACTGAATCGC ATTGGGGGTG TGATGCAAGA TGCTCTCCCC 
ATTCAAAACT ACAGATCCAA AGAAGGTTGG GATTTCCATT CTCACTTCAT TCTCTATTGT 
TTGGAACATG GTCTGCAGTA TCCTCTCTAT GTCTATCTTG ACTTTTACAA ACTTAGTCCT 
GCAAATTGTC CCTTTTTGGA AAAAAAAGAA TTACATGAAA CTCACCCTTG GTTTGAATTT 
TTGGTTCAGT GTCGACAGGT TTCCAGTAAT TTAACAGATC CCAAACTGAT CTTCCAGGCT 
AGCCTTGCAA ACGCTCAGAT ACTGATTCCC AGCAACCAGG CCAGTGTAAG CAGTATGCTA 
TTGGAAGGAC ATACTCTCCT GGCCCTTGCT ACCACAATGT ATGCCCCTGG CGGTGTCAGC 
CAGGTTGTTC AGGATGATGA AAATGAGACC TGTTTGAAGA AAGTAGATCC CCAGTTACTG 
AAGATGGCAT TAACTCCTTA TCCCAAGCTA AAAACTGCTC TCTTCCCACA GTACACTACC 
TCTAATGTTC TGCCGCCTGA TATTACGCTT TACCACCTTA TTCAGTCACT ATTGCCCTTT 
GACTCTAGCA GATTGTTTGG CTGGCAGTCT GCCAACACAC TGGCTATTGG AGATGCAGTG 
AGTCATCTCC CACATTTTTC TAGCCCTGAT CTGGTTAATA AATATGCTAT CATGGAACGT 
CTGAATTTTG CATATTATTT ACAACATGGA CGGCCGTCAT TTGCATTTGG TACTTTTCTG 
GTCCAGGAAT TAACCAAGAG CAAGACTCCC AAGCAGCTGA TCCAGCAAGT AGGCAATGAA 
GCTTATGTTT TAGGGCTCTC CTCCTTCTAC ATTCCATCAG TAGGAGCTGC CTGTGTTTGT 
TTCTTAGAAC TGCTTGGCCT CGATAGCCTC AAGCTCAGAG TTGACATGAA AGTGGCCAAT 
ATAATTTTGA ACTACAAATG TAGAAATGAA GATGTTCAGT ACAGCTGTAT CAGAGAGGCT 
CTAGTTGAAA AACTATCTAA ACTGGCTGTG GGTGAAAAGG CCACCGTAGA GGAATTGCTT 
ATTCTCTTAG AAGAAGGTAT ATGGAACAAC ATTCAGCAGC AAGAGATAAA GAGGTTATCT 
AGTGAATCTA GCAGCCAGTG GGCATTAGCA GTACAGTTCT GCAGGCTTCA CAATGTGAAG 
CTGAGCACGT CTTACCTTAG AGAATGTGCC AAAGCAAATG ACTGGTTACA GTTCATTATT 
CACAGCCAAC TCTATAACTA TCACCCAGCG GAGGTGAAAT CTCTTCTTCA GTATTTCAGT 
CCTGTCCTTC AAGACCACTT AAGACTAGCT TTTGAGAACC TGACCTCAGT GTCTAAATCC 
AAAATGGATA GTGGTGAAGT CTGCAGCAGG TCTCCCCAGG AACTCCAGAG AAACAAAGAA 
GAAATGGCTG ATTTCTTTCA AGTTCTACTC CAGTGCTCAG AGGAACCAAA CCCCTGGTGC 
TGGCTCCTAG CTGAAGCAAT GAAACAACAG GCTCCTATCC TTAGTGTCCT GGCCTCATGT 
CTTCAGGATG CCAGTACTAT TCCTTGTCTC TGTGTTTGGA TCATCACATC TGTGGAGGAC 
AGTGTTGCAG CTGAAGCAAC AGAACACATT CAGGGCTCAG TGGAAGGCCA CATATGGGAC 
CTTCAAGACC TCTCAGTCAT CTGGAGAACA TTATTAACAA GGCAGAAGAA CAAAACTCTC 
ATCAGAGGTT TCCAACTTTT CTTTAAGGAT TCCCCACTAC TGTTGATGAT GGAGATGTAT 
GAACTGTGTA TGTTCTTCAA GAATTATAAA GAAGCTGAAG CTAAACTTCT GGAGTTCCAA 
AAGAGCTTTG AAACTGTTGA AACAGCAGCC ACAAGGGTCC ACACTACCAT CCCTGCCCCA 
TGGCTGAGGG AGCAGGTGTG TTTTCTTCTG AAGCTTATGC TGCAGCAGTG TGGGACCCAG 
TATGAACTGG GGAAGCTTTT ACAGCTATTT GTTGGAATAG AAAATTTCTT CTCTGATGGT 
CCAGATGTGA AGAAGCTCTG TGTCCTTAGT GAAATTTTGA AGGACACATC CATAGCCATT 
AATCATGTAA TTATTAGCAG CCACAGCCTT GAGAATTTTC AGCATGAATG TACATCTATT 
TTGGAAAAAC TGGCGATGGA GGGACAGTTT GCTTTGGCCA GGAGAGTAGC AGAATTAGCT 
GAATTACCTG TGGACAACTT GGTTATTGAA GAGATGACAC AGGAAATGCA AACCTTAAAA 
CACATTGACC AGTGGTCCCT GAAACAAGCA AGAATTGACT TCTGGAAAAA ATGTCATGAG 
AATTTTAAGA AAAATTCCAT TTCAAGCAAA GCAGCTTCTT CCTTTTTCTC AGCCCAGGCA 
CCTGAGTGCC CAACTGAAGA GGGTCGGAGC AGCAGTGAGG AGCGCCATCT GCTGCTCACA 
TTGGCAGCAC ACTGGCTTGC TCAGGAGGAT CTGGTGCCGG TGGCCGAACT GGAGGAGCTA 
GAGAAGCAGA TCTGGCTCTG CCGCATTACC CAATACACCC TCAGAGAAAA ACAGGAGCAA 
GTAGAGCTCG GGTTTTCTCA ACAGATTTCA ACTAGTGGGG AACTTTCCTT TGATGGCATA 
GCCAGTAAGT TTTCACTCTC CATGTTGGCT GTTCTGAACA CATCAAAGTA CTTAGAACTC 
AGTGGCTTTC CATCCAAAGA GACATGTGAT AGCGGACTGG ATTGGAAGGA GCAGGAGTCA 
CTGAACTTTC TGATTGGGCA CCTATTGGAT GATGGCTTTG TGCATGAAGC AAGTAGAGTG 
TGTCGGTATT TTCATTTCTA TAACCAAGAT GTTGCCTTGG TGTTGCACTG CAGAGCACTG 
GCCTCAGGGG AAGCTAGTGT GGATGACCTG CACCCAGAGA TCCATGCTCT CCTACAAAGT 
GCTGAGCCGC TTGAGGAGGA GGAAGCTGGC ATTCCACTAA GAAAAGTCCA GAGCAGTTCA 
AGTTTGGATA GTCAGTCATT TGTGATGCTG CCCCCCAGTG ATGAAGTGGT GACTAATCTG 
GAAACTCTGA CAAGTAAATG CCTCCATGGG AAGAACTACT GTCGGCAGGT CCTGTGTCTG 
TATGAACTTG CCAAGGAGTT GGGCTGTTCC TACACAGACG TGGCCTCTCA GGACGGGGAG 
GCCATGCTCC GGGCAATCTT GGCTTCCCAG CAGCCCGACC GATGCAAGCG AGCCCAGGCT 
TTCATTAGCA CCCAGGGCCT CGAGGCAGAC ACGGTGGCTG AACTTGTGGC TGAAGAGGTG 
ACCCGGGAGC TGCTGACCCC ACCGGAGGGA ACAGGACATC AGCAGATGTT TGCCCCAGCA 
GAGGAAAGCC GGACATTTCT TCAGCTGACC ACTCTGTGTC AAGACCGCAC ACTGGTAGGC 
ATGAAGTTGT TGGACAAGAT TTCGTCCGTT CCCCATGGAG AATTATCTTG CACCACAGAG 
CTGCTGATCC TGGCCCATCA CTGCTTCACA CTGACATGCC ACATGGAGGG CATCATCCGA 
GTCCTGCAAG CTGCCCGGCT GCTCACGGAC AAACACTTGG CCCCCAATGA AGAGTACGGG 
CTGATGGTAC GCCTCCTCAC CGGCATCGGA AGGTACAACG AGATGACGTA CATATTTGAC 
TTGCTGCATA ATAAGCACTA TTTTGAAGTG CTGATGAGGA AGAAGTTGGA TCCGAGTGGC 
TCCCTGAAGA CCGCCCTACT GGACTACATC AAACGCTGCC GCCCTGGGGA CAGTGAGAAG 
CACAACATGA TCGCTCTGTG CTTCAGCATG TGTCGGGAGA TTGGGGAGAA CCATGAGGCA 
GCTGCCCGCA TCCAGCTGAA ACTGATCGAG TCTCAGCCCT GGGAGGACAG CCTCAAGGAC 
GGGCACCACC TGAAGCAGCT GCTGCTGAAA GCCCTGACTC TGATGCTGGA TGCAGCCGAG 
AGCTATGCCA AGGACTCCTG TGTGCGACAG GCCCAGCACT GTCACCGGCT CACCAAGTTG 
ATAACACTGC AGATTCATTT TCTGAACACT GGCCAGAACA CAATGCTCAT CAACCTGGGC 
CGCCACCGGC TGATGGACTG TATCCTCGCT CTACCTCGGT TCTACCAGGC TTCTATTGTG 
GCCGAAGCCT ATGACTTTGT TCCTGATTGG GCAGAAATCC TCTACCAACA AGTGATTCTT 
AAAGGAGACT TTAATTACCT GGAAGAATTT AAGCAGCAAA GGTTATTAAG ATCCAGTATA 
TTTGAAGAGA TTTCAAAAAA ATATAAACAA CATCAGCCGA CAGACACGGC TGTGAAAAAC 
CTGAAGAAGT TACTCACATA CTGTGAAGAT GTCTACTTGT ACTACAAGCT CGCATATGAA 
CACAAATTTT ATGACATTGT GAATGTGCTT CTGAAGGACC CTCAGAGCGG CTGCTGTCTA 
AAGGACATGC TAGCAGGTTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013964966.1
CDS	21..7121
Translation	MAAGEAAARAAGCSSCGAETMERVLPMLLVPVPVEATGRLGSRAQLHREQEVLGSLTAAGSLQVLSLAPGGRSGGACCLNGPFRHFIWEDSHNGSSPTDKPKLLALSENYELFIYEFNLKDGRCDATILYSCSEETLQKLIEDQNINIFDIVDGRHVAHVDLALHQEDMCNEQQQELAKISSFTSLKVSQDLDVVVIVSSFNSAVALNLNLYFRQHPGHLLCERTLEDIPIEGPEGIDEDDPVNSDYNMKLTKFSFQVDRSWKAQLSSLNEIIKSSKWEVPCCAPWFQNILHLESPESGYHSTSTPGWAFIPQDEIHSHYKFPQKDHANTGDPGRSWKIMNISELKEPVELTCMSVTGFSVLFTWAVEKMGCTIVLWDLETQGIQCFSLGKRCIPIDMSGDQQLCLVLTENGLSLILFGLTQEEFLNRLMIHGSASTVDSLCHLNGWGRCSIPIHALEAGIENRQLDTVDFFLKSKENLLNPSSKSSVPDQFDDFPSHLYLRNVEKLTPALDLLCSAIRESDSETQSKHFSEQLLNLTLSFLNKQIKELFVHNEELDEHLQKGVNILTSYINELRTFMIKFPWKIVDPVDEYDVSENVLKVRESDTWEKLSIEEVIANAILNNKIPEAQTFFRINNHSAQRLEELIRIGLNLVFDCLKKNNIKEASELLKNMGFDVKDQLLKICFYTTDKNIRDFLVEILKEKNYFSENEKRTIDFVHQVEKFYSGHFQENVQIQSFTRYWIKEQDFFKHKSVLDSLLKYDPKDKFNKQDHRIVLNWAQWWNQLTQESILLPRISPEEYKSCSPEALWSYLTAHHDWSSISLWIEEFQAQNSCVLLQQNKWPLLTVDVIDQNTCCNNYMRNEILDQLARNGIFLQSELEDFELFLLRLNRIGGVMQDALPIQNYRSKEGWDFHSHFILYCLEHGLQYPLYVYLDFYKLSPANCPFLEKKELHETHPWFEFLVQCRQVSSNLTDPKLIFQASLANAQILIPSNQASVSSMLLEGHTLLALATTMYAPGGVSQVVQDDENETCLKKVDPQLLKMALTPYPKLKTALFPQYTTSNVLPPDITLYHLIQSLLPFDSSRLFGWQSANTLAIGDAVSHLPHFSSPDLVNKYAIMERLNFAYYLQHGRPSFAFGTFLVQELTKSKTPKQLIQQVGNEAYVLGLSSFYIPSVGAACVCFLELLGLDSLKLRVDMKVANIILNYKCRNEDVQYSCIREALVEKLSKLAVGEKATVEELLILLEEGIWNNIQQQEIKRLSSESSSQWALAVQFCRLHNVKLSTSYLRECAKANDWLQFIIHSQLYNYHPAEVKSLLQYFSPVLQDHLRLAFENLTSVSKSKMDSGEVCSRSPQELQRNKEEMADFFQVLLQCSEEPNPWCWLLAEAMKQQAPILSVLASCLQDASTIPCLCVWIITSVEDSVAAEATEHIQGSVEGHIWDLQDLSVIWRTLLTRQKNKTLIRGFQLFFKDSPLLLMMEMYELCMFFKNYKEAEAKLLEFQKSFETVETAATRVHTTIPAPWLREQVCFLLKLMLQQCGTQYELGKLLQLFVGIENFFSDGPDVKKLCVLSEILKDTSIAINHVIISSHSLENFQHECTSILEKLAMEGQFALARRVAELAELPVDNLVIEEMTQEMQTLKHIDQWSLKQARIDFWKKCHENFKKNSISSKAASSFFSAQAPECPTEEGRSSSEERHLLLTLAAHWLAQEDLVPVAELEELEKQIWLCRITQYTLREKQEQVELGFSQQISTSGELSFDGIASKFSLSMLAVLNTSKYLELSGFPSKETCDSGLDWKEQESLNFLIGHLLDDGFVHEASRVCRYFHFYNQDVALVLHCRALASGEASVDDLHPEIHALLQSAEPLEEEEAGIPLRKVQSSSSLDSQSFVMLPPSDEVVTNLETLTSKCLHGKNYCRQVLCLYELAKELGCSYTDVASQDGEAMLRAILASQQPDRCKRAQAFISTQGLEADTVAELVAEEVTRELLTPPEGTGHQQMFAPAEESRTFLQLTTLCQDRTLVGMKLLDKISSVPHGELSCTTELLILAHHCFTLTCHMEGIIRVLQAARLLTDKHLAPNEEYGLMVRLLTGIGRYNEMTYIFDLLHNKHYFEVLMRKKLDPSGSLKTALLDYIKRCRPGDSEKHNMIALCFSMCREIGENHEAAARIQLKLIESQPWEDSLKDGHHLKQLLLKALTLMLDAAESYAKDSCVRQAQHCHRLTKLITLQIHFLNTGQNTMLINLGRHRLMDCILALPRFYQASIVAEAYDFVPDWAEILYQQVILKGDFNYLEEFKQQRLLRSSIFEEISKKYKQHQPTDTAVKNLKKLLTYCEDVYLYYKLAYEHKFYDIVNVLLKDPQSGCCLKDMLAG

Target ORF information:

RefSeq Version	XM_014109491.2
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris SPG11, spatacsin vesicle trafficking associated (SPG11), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014109491.2

ORF Insert Sequence:

ATGGCCGCAG GAGAGGCAGC TGCGAGAGCT GCTGGTTGCA GCAGCTGCGG CGCTGAAACT 
ATGGAACGGG TTCTACCTAT GCTGTTGGTG CCGGTCCCTG TGGAGGCAAC CGGGCGGTTG 
GGCTCCCGGG CGCAGTTGCA CAGAGAGCAG GAGGTTCTGG GGAGCCTAAC AGCTGCAGGC 
AGCCTGCAGG TGCTTTCCTT GGCGCCGGGC GGCCGGAGCG GCGGGGCCTG CTGCCTCAAT 
GGCCCCTTTC GGCACTTTAT ATGGGAGGAT TCTCATAACG GCAGCTCACC AACAGACAAG 
CCCAAACTTC TTGCTCTTAG TGAAAACTAT GAACTGTTTA TCTATGAATT TAATTTGAAA 
GATGGAAGAT GTGATGCAAC CATTTTGTAT AGCTGTAGTG AGGAGACATT GCAGAAGCTC 
ATTGAAGATC AAAATATCAA CATTTTTGAC ATTGTGGATG GAAGACATGT AGCTCACGTG 
GATTTAGCAC TTCACCAAGA AGATATGTGT AATGAACAGC AACAGGAACT AGCCAAGATT 
TCTTCCTTTA CTTCACTGAA AGTGTCTCAA GACCTAGATG TTGTAGTGAT TGTCAGCTCC 
TTCAACTCTG CAGTTGCTCT TAACCTAAAC TTGTATTTCA GGCAACACCC AGGACACCTA 
CTGTGTGAAA GAACTCTAGA AGACATTCCT ATTGAAGGCC CTGAGGGGAT CGATGAAGAT 
GATCCTGTTA ATTCTGACTA CAACATGAAA CTGACCAAGT TTTCCTTTCA AGTTGATAGG 
TCTTGGAAAG CCCAGCTATC ATCACTGAAT GAAATAATAA AGAGCTCCAA ATGGGAGGTT 
CCCTGTTGTG CTCCATGGTT CCAGAATATT TTGCATTTGG AGTCACCGGA ATCTGGTTAC 
CACAGTACTA GTACACCAGG CTGGGCCTTC ATCCCACAGG ATGAAATTCA TAGCCACTAC 
AAGTTTCCAC AGAAAGATCA TGCCAACACC GGTGACCCAG GAAGATCATG GAAAATAATG 
AACATTAGTG AACTAAAGGA ACCTGTTGAG CTTACATGTA TGTCTGTGAC AGGATTCAGT 
GTACTGTTTA CTTGGGCAGT GGAAAAGATG GGCTGCACCA TTGTCCTTTG GGATTTGGAG 
ACCCAGGGTA TCCAGTGTTT TTCCCTTGGC AAAAGGTGTA TTCCTATAGA TATGAGTGGA 
GACCAGCAGC TGTGCCTTGT TTTGACAGAA AATGGACTCT CCCTGATTTT GTTTGGATTG 
ACTCAGGAGG AGTTTTTAAA TAGACTAATG ATCCATGGAA GTGCCAGCAC TGTGGACTCT 
CTTTGCCATC TCAATGGTTG GGGGAGGTGC TCAATCCCCA TACATGCGCT GGAGGCTGGG 
ATAGAAAATC GTCAGCTGGA CACCGTAGAT TTCTTTTTGA AGAGCAAAGA AAATCTTTTG 
AATCCATCCT CAAAATCTTC CGTACCGGAT CAGTTTGATG ACTTTCCATC TCATTTATAT 
TTAAGAAATG TGGAAAAGCT GACACCAGCA TTGGATTTAC TTTGCTCAGC AATTAGAGAA 
AGTGATTCTG AAACCCAAAG CAAACACTTT TCAGAACAGT TGCTTAACCT TACACTGTCT 
TTTCTTAACA AACAAATAAA GGAGCTTTTT GTTCACAATG AAGAATTAGA TGAACATCTA 
CAGAAAGGAG TGAACATTTT GACCAGCTAC ATTAATGAAC TTCGAACCTT CATGATAAAA 
TTTCCTTGGA AGATAGTAGA CCCAGTAGAT GAATATGATG TAAGTGAAAA TGTCCTCAAA 
GTAAGAGAGA GTGATACATG GGAGAAACTC AGCATTGAGG AAGTTATTGC CAATGCTATT 
TTAAACAACA AAATACCAGA GGCACAGACT TTCTTCAGGA TTAACAATCA TTCTGCTCAA 
AGACTTGAGG AGCTGATTAG AATAGGCCTA AATTTGGTCT TTGACTGTTT GAAAAAGAAC 
AATATAAAGG AAGCATCTGA ACTTTTGAAG AATATGGGCT TTGATGTAAA AGACCAGTTG 
CTCAAGATAT GTTTCTATAC AACTGATAAA AATATACGGG ACTTTTTGGT GGAAATTTTA 
AAAGAAAAAA ATTATTTTTC TGAAAATGAA AAAAGAACTA TAGACTTCGT GCATCAAGTT 
GAGAAGTTTT ATTCAGGACA TTTTCAAGAA AATGTGCAGA TCCAGTCCTT TACCAGGTAT 
TGGATAAAGG AACAGGATTT TTTCAAGCAC AAATCTGTTT TGGACTCACT CCTGAAATAT 
GATCCTAAAG ATAAATTTAA CAAACAGGAC CATAGAATTG TGTTAAATTG GGCTCAGTGG 
TGGAATCAAC TAACACAAGA ATCCATCCTT CTCCCCAGGA TAAGTCCAGA AGAATACAAA 
TCATGTTCTC CTGAAGCCCT TTGGAGCTAT CTCACAGCCC ATCATGATTG GTCAAGCATT 
AGCTTGTGGA TTGAAGAATT TCAGGCCCAG AACAGTTGTG TTTTACTGCA GCAGAACAAA 
TGGCCCCTTC TGACTGTTGA TGTTATAGAC CAGAATACTT GCTGCAACAA CTACATGAGG 
AATGAAATTT TAGATCAGCT GGCCAGGAAT GGGATTTTTC TTCAATCTGA GCTAGAAGAC 
TTTGAACTCT TCCTGCTAAG ACTGAATCGC ATTGGGGGTG TGATGCAAGA TGCTCTCCCC 
ATTCAAAACT ACAGATCCAA AGAAGGTTGG GATTTCCATT CTCACTTCAT TCTCTATTGT 
TTGGAACATG GTCTGCAGTA TCCTCTCTAT GTCTATCTTG ACTTTTACAA ACTTAGTCCT 
GCAAATTGTC CCTTTTTGGA AAAAAAAGAA TTACATGAAA CTCACCCTTG GTTTGAATTT 
TTGGTTCAGT GTCGACAGGT TTCCAGTAAT TTAACAGATC CCAAACTGAT CTTCCAGGCT 
AGCCTTGCAA ACGCTCAGAT ACTGATTCCC AGCAACCAGG CCAGTGTAAG CAGTATGCTA 
TTGGAAGGAC ATACTCTCCT GGCCCTTGCT ACCACAATGT ATGCCCCTGG CGGTGTCAGC 
CAGGTTGTTC AGGATGATGA AAATGAGACC TGTTTGAAGA AAGTAGATCC CCAGTTACTG 
AAGATGGCAT TAACTCCTTA TCCCAAGCTA AAAACTGCTC TCTTCCCACA GTACACTACC 
TCTAATGTTC TGCCGCCTGA TATTACGCTT TACCACCTTA TTCAGTCACT ATTGCCCTTT 
GACTCTAGCA GATTGTTTGG CTGGCAGTCT GCCAACACAC TGGCTATTGG AGATGCAGTG 
AGTCATCTCC CACATTTTTC TAGCCCTGAT CTGGTTAATA AATATGCTAT CATGGAACGT 
CTGAATTTTG CATATTATTT ACAACATGGA CGGCCGTCAT TTGCATTTGG TACTTTTCTG 
GTCCAGGAAT TAACCAAGAG CAAGACTCCC AAGCAGCTGA TCCAGCAAGT AGGCAATGAA 
GCTTATGTTT TAGGGCTCTC CTCCTTCTAC ATTCCATCAG TAGGAGCTGC CTGTGTTTGT 
TTCTTAGAAC TGCTTGGCCT CGATAGCCTC AAGCTCAGAG TTGACATGAA AGTGGCCAAT 
ATAATTTTGA ACTACAAATG TAGAAATGAA GATGTTCAGT ACAGCTGTAT CAGAGAGGCT 
CTAGTTGAAA AACTATCTAA ACTGGCTGTG GGTGAAAAGG CCACCGTAGA GGAATTGCTT 
ATTCTCTTAG AAGAAGGTAT ATGGAACAAC ATTCAGCAGC AAGAGATAAA GAGGTTATCT 
AGTGAATCTA GCAGCCAGTG GGCATTAGCA GTACAGTTCT GCAGGCTTCA CAATGTGAAG 
CTGAGCACGT CTTACCTTAG AGAATGTGCC AAAGCAAATG ACTGGTTACA GTTCATTATT 
CACAGCCAAC TCTATAACTA TCACCCAGCG GAGGTGAAAT CTCTTCTTCA GTATTTCAGT 
CCTGTCCTTC AAGACCACTT AAGACTAGCT TTTGAGAACC TGACCTCAGT GTCTAAATCC 
AAAATGGATA GTGGTGAAGT CTGCAGCAGG TCTCCCCAGG AACTCCAGAG AAACAAAGAA 
GAAATGGCTG ATTTCTTTCA AGTTCTACTC CAGTGCTCAG AGGAACCAAA CCCCTGGTGC 
TGGCTCCTAG CTGAAGCAAT GAAACAACAG GCTCCTATCC TTAGTGTCCT GGCCTCATGT 
CTTCAGGATG CCAGTACTAT TCCTTGTCTC TGTGTTTGGA TCATCACATC TGTGGAGGAC 
AGTGTTGCAG CTGAAGCAAC AGAACACATT CAGGGCTCAG TGGAAGGCCA CATATGGGAC 
CTTCAAGACC TCTCAGTCAT CTGGAGAACA TTATTAACAA GGCAGAAGAA CAAAACTCTC 
ATCAGAGGTT TCCAACTTTT CTTTAAGGAT TCCCCACTAC TGTTGATGAT GGAGATGTAT 
GAACTGTGTA TGTTCTTCAA GAATTATAAA GAAGCTGAAG CTAAACTTCT GGAGTTCCAA 
AAGAGCTTTG AAACTGTTGA AACAGCAGCC ACAAGGGTCC ACACTACCAT CCCTGCCCCA 
TGGCTGAGGG AGCAGGTGTG TTTTCTTCTG AAGCTTATGC TGCAGCAGTG TGGGACCCAG 
TATGAACTGG GGAAGCTTTT ACAGCTATTT GTTGGAATAG AAAATTTCTT CTCTGATGGT 
CCAGATGTGA AGAAGCTCTG TGTCCTTAGT GAAATTTTGA AGGACACATC CATAGCCATT 
AATCATGTAA TTATTAGCAG CCACAGCCTT GAGAATTTTC AGCATGAATG TACATCTATT 
TTGGAAAAAC TGGCGATGGA GGGACAGTTT GCTTTGGCCA GGAGAGTAGC AGAATTAGCT 
GAATTACCTG TGGACAACTT GGTTATTGAA GAGATGACAC AGGAAATGCA AACCTTAAAA 
CACATTGACC AGTGGTCCCT GAAACAAGCA AGAATTGACT TCTGGAAAAA ATGTCATGAG 
AATTTTAAGA AAAATTCCAT TTCAAGCAAA GCAGCTTCTT CCTTTTTCTC AGCCCAGGCA 
CCTGAGTGCC CAACTGAAGA GGGTCGGAGC AGCAGTGAGG AGCGCCATCT GCTGCTCACA 
TTGGCAGCAC ACTGGCTTGC TCAGGAGGAT CTGGTGCCGG TGGCCGAACT GGAGGAGCTA 
GAGAAGCAGA TCTGGCTCTG CCGCATTACC CAATACACCC TCAGAGAAAA ACAGGAGCAA 
GTAGAGCTCG GGTTTTCTCA ACAGATTTCA ACTAGTGGGG AACTTTCCTT TGATGGCATA 
GCCAGTAAGT TTTCACTCTC CATGTTGGCT GTTCTGAACA CATCAAAGTA CTTAGAACTC 
AGTGGCTTTC CATCCAAAGA GACATGTGAT AGCGGACTGG ATTGGAAGGA GCAGGAGTCA 
CTGAACTTTC TGATTGGGCA CCTATTGGAT GATGGCTTTG TGCATGAAGC AAGTAGAGTG 
TGTCGGTATT TTCATTTCTA TAACCAAGAT GTTGCCTTGG TGTTGCACTG CAGAGCACTG 
GCCTCAGGGG AAGCTAGTGT GGATGACCTG CACCCAGAGA TCCATGCTCT CCTACAAAGT 
GCTGAGCCGC TTGAGGAGGA GGAAGCTGGC ATTCCACTAA GAAAAGTCCA GAGCAGTTCA 
AGTTTGGATA GTCAGTCATT TGTGATGCTG CCCCCCAGTG ATGAAGTGGT GACTAATCTG 
GAAACTCTGA CAAGTAAATG CCTCCATGGG AAGAACTACT GTCGGCAGGT CCTGTGTCTG 
TATGAACTTG CCAAGGAGTT GGGCTGTTCC TACACAGACG TGGCCTCTCA GGACGGGGAG 
GCCATGCTCC GGGCAATCTT GGCTTCCCAG CAGCCCGACC GATGCAAGCG AGCCCAGGCT 
TTCATTAGCA CCCAGGGCCT CGAGGCAGAC ACGGTGGCTG AACTTGTGGC TGAAGAGGTG 
ACCCGGGAGC TGCTGACCCC ACCGGAGGGA ACAGGACATC AGCAGATGTT TGCCCCAGCA 
GAGGAAAGCC GGACATTTCT TCAGCTGACC ACTCTGTGTC AAGACCGCAC ACTGGTAGGC 
ATGAAGTTGT TGGACAAGAT TTCGTCCGTT CCCCATGGAG AATTATCTTG CACCACAGAG 
CTGCTGATCC TGGCCCATCA CTGCTTCACA CTGACATGCC ACATGGAGGG CATCATCCGA 
GTCCTGCAAG CTGCCCGGCT GCTCACGGAC AAACACTTGG CCCCCAATGA AGAGTACGGG 
CTGATGGTAC GCCTCCTCAC CGGCATCGGA AGGTACAACG AGATGACGTA CATATTTGAC 
TTGCTGCATA ATAAGCACTA TTTTGAAGTG CTGATGAGGA AGAAGTTGGA TCCGAGTGGC 
TCCCTGAAGA CCGCCCTACT GGACTACATC AAACGCTGCC GCCCTGGGGA CAGTGAGAAG 
CACAACATGA TCGCTCTGTG CTTCAGCATG TGTCGGGAGA TTGGGGAGAA CCATGAGGCA 
GCTGCCCGCA TCCAGCTGAA ACTGATCGAG TCTCAGCCCT GGGAGGACAG CCTCAAGGAC 
GGGCACCACC TGAAGCAGCT GCTGCTGAAA GCCCTGACTC TGATGCTGGA TGCAGCCGAG 
AGCTATGCCA AGGACTCCTG TGTGCGACAG GCCCAGCACT GTCACCGGCT CACCAAGTTG 
ATAACACTGC AGATTCATTT TCTGAACACT GGCCAGAACA CAATGCTCAT CAACCTGGGC 
CGCCACCGGC TGATGGACTG TATCCTCGCT CTACCTCGGT TCTACCAGGC TTCTATTGTG 
GCCGAAGCCT ATGACTTTGT TCCTGATTGG GCAGAAATCC TCTACCAACA AGTGATTCTT 
AAAGGAGACT TTAATTACCT GGAAGAATTT AAGCAGCAAA GGTTATTAAG ATCCAGTATA 
TTTGAAGAGA TTTCAAAAAA ATATAAACAA CATCAGCCGA CAGACACGGC TGTGAAAAAC 
CTGAAGAAGT TACTCACATA CTGTGAAGAT GTCTACTTGT ACTACAAGCT CGCATATGAA 
CACAAATTTT ATGACATTGT GAATGTGCTT CTGAAGGACC CTCAGAGCGG CTGCTGTCTA 
AAGGACATGC TAGCAGGTTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

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SPG11 cDNA ORF clone, Canis lupus familiaris(dog)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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