Spg11 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Spg11
Entrez Gene ID	311372
Full Name	SPG11, spatacsin vesicle trafficking associated
Synonyms	RGD1562529
General protein information	Preferred Names SPG11, spatacsin vesicle trafficking associated Names spatacsin spastic paraplegia 11 (autosomal recessive)
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 3 Map Location: 3q35

mRNA and Protein(s)

mRNA	Protein	Name
NM_001191571.1	NP_001178500.1	spatacsin
XM_006234855.3	XP_006234917.2	spatacsin isoform X2
XM_017591740.1	XP_017447229.1	spatacsin isoform X1

Pathways from BioSystems

Homologies

Rattus norvegicus (Norway rat)	Spg11	NP_001178500.1
Homo sapiens (human)	SPG11	NP_079413.3
Bos taurus (cattle)	SPG11	XP_002691138.2
Mus musculus (house mouse)	Spg11	NP_663506.2
Canis lupus familiaris (dog)	SPG11	XP_003640073.2
Macaca mulatta (Rhesus monkey)	LOC712221	XP_002808495.1
Gallus gallus (chicken)	SPG11	XP_003641879.2
Danio rerio (zebrafish)	spg11	NP_001181923.1

Gene Ontology
Bibliography

Related articles in PubMed

In Vivo Evidence for Lysosome Depletion and Impaired Autophagic Clearance in Hereditary Spastic Paraplegia Type SPG11.
Varga RE, Khundadze M, Damme M, Nietzsche S, Hoffmann B, Stauber T, Koch N, Hennings JC, Franzka P, Huebner AK, Kessels MM, Biskup C, Jentsch TJ, Qualmann B, Braulke T, Kurth I, Beetz C, Hübner CA
PLoS genetics11(8)e1005454(2015 Aug)

Dysfunction of spatacsin leads to axonal pathology in SPG11-linked hereditary spastic paraplegia.
Pérez-Brangulí F, Mishra HK, Prots I, Havlicek S, Kohl Z, Saul D, Rummel C, Dorca-Arevalo J, Regensburger M, Graef D, Sock E, Blasi J, Groemer TW, Schlötzer-Schrehardt U, Winkler J, Winner B
Human molecular genetics23(18)4859-74(2014 Sep)

Cellular distribution and subcellular localization of spatacsin and spastizin, two proteins involved in hereditary spastic paraplegia.
Murmu RP, Martin E, Rastetter A, Esteves T, Muriel MP, El Hachimi KH, Denora PS, Dauphin A, Fernandez JC, Duyckaerts C, Brice A, Darios F, Stevanin G
Molecular and cellular neurosciences47(3)191-202(2011 Jul)

Integral and associated lysosomal membrane proteins.
Schröder B, Wrocklage C, Pan C, Jäger R, Kösters B, Schäfer H, Elsässer HP, Mann M, Hasilik A
Traffic (Copenhagen, Denmark)8(12)1676-86(2007 Dec)

Mutations in SPG11, encoding spatacsin, are a major cause of spastic paraplegia with thin corpus callosum.
Stevanin G, Santorelli FM, Azzedine H, Coutinho P, Chomilier J, Denora PS, Martin E, Ouvrard-Hernandez AM, Tessa A, Bouslam N, Lossos A, Charles P, Loureiro JL, Elleuch N, Confavreux C, Cruz VT, Ruberg M, Leguern E, Grid D, Tazir M, Fontaine B, Filla A, Bertini E, Durr A, Brice A
Nature genetics39(3)366-72(2007 Mar)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Spg11 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Spg11 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ORa07025	NM_001191571.1 Latest version!	Rattus norvegicus SPG11 vesicle trafficking associated, spatacsin (Spg11), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
ORa60399	XM_017591740.1 Latest version!	Rattus norvegicus spastic paraplegia 11 (autosomal recessive) (Spg11), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1301.30 $1859.00
ORa60400	XM_006234855.3 Latest version!	Rattus norvegicus spastic paraplegia 11 (autosomal recessive) (Spg11), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1301.30 $1859.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Spg11 ( NM_001191571.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_001178500.1 Rattus norvegicus SPG11 vesicle trafficking associated, spatacsin (Spg11), mRNA.

Price & Availability↑

CloneID	ORa07025
Clone ID Related Accession (Same CDS sequence)	NM_001191571.1
Accession Version	NM_001191571.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6948bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1554652800000
Organism	Rattus norvegicus(Norway rat)
Product	spatacsin
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AC112350.4. On Jul 17, 2010 this sequence version replaced XM_002729179.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## RNAseq introns :: mixed/partial sample support SAMEA2689601, SAMEA2689603 [ECO:0000350] ##Evidence-Data-END##

ATGGCTGCCA AGCCAGGGCC CGGAAGTGCT GCCTCCCCCG GCTGTGCCGG CGCCATGGAG 
CGAGTTCTAC CCATGCTGCT GGTGCGGATC CCTGCCGAGG AGACGGCGCA GCTGGGACCG 
CGGGCGCAGC TGCACCGAGA GCTGGAGGCC CTGGGGAGCC TGACGGCTGC GGGCAGTCTC 
CAAGTGCTTT CCTTGGCGCC GGGCAGTAGG GGTGGAAGCA GCTGCCGCCT CCAAGGCCCC 
TTTAGGCAGT TCCTGTGGGA GGAGTCTCAG AACAGCACAT CAGCAGACAA GCCCAGAGTG 
CTTGTTGCTC GAGAAGATTG TGGGCTGCTC ATATATGAAT TTGACTTGAG AGATGGAAGA 
TGTGACGCAG CCTTACTACA CAGCTGTTGT GGGAGGACCC TGCAGAAGCT CACTGAAGAT 
CAAGGTGTCA GCATTTCCTT CAAGTCTTTG AGGATTCTGT CATTTGACAA CAGCACGTCC 
TTACTGCTCA TCAATGAGTG TCTCGTCGTA CGTGTCGTGT TTCCGGGGAG AGAATCTGGG 
ATTGGAGTGC TGGGCTGCCT CTCACTGCCC TTGCCTGCAC AGGCAGTGGA CACGATAATT 
GACGTGCAGC TCTGCAAGAG GTTTCTTTTC CTTCTGAGTA CATTAGGCTG GATCCATATT 
TTTGACACTG TAAAGGGCAT GCATGTAGCC CGCGTGGACT TGGCTCTTCC CCGAGAAGAT 
TCTGAGCAGC AGGAGCCTGC CAGCATTTCT TCCTTTATGT CACTGAAAGT GTCTCGGGAC 
TTAGATGTTT TGCTACTCGT CAGCTCGTCC AGTACTGCAG TTGCTCTTAA CCTTCACTTG 
TATTTCAGGC TACACCCAGG GCACCTGCTG TGTGAAGGAA CTCTGCAAGA CCTCCCTATT 
GAAGGGCCTA CAGGAATAGA TGAAGATGAC CTTGTTAATT CTGCCCACAA CATGAAACTG 
ACCAAGGTGT CCTTCCAAGT TGATAGGTCC TGGAGAGCCC AGCTGGCATC ACTGAATGAA 
TCTGTGAGGA GCTCAGAACC AGAGCTTTCC TGCTATCCTC CGTGGTTCCA GGGTGCTTTG 
TGTTTGGAGT CCCTGGAATC TGGTAACCAC TCCCCCATTG TGCCAAACCA CGTTTTCATT 
CCAGGGGATG TCCCGAGTGG CCAGTATGCT TTTCCTCAGA AGGAGCACAG CAAGACCAGT 
GAGCCAAGGC CGTGGAGGAC AATGCACCTG AGTGAACAAG AGCAGCCCAC AGAGCTCACG 
TGTCTGTCTG TGACGGGATT TACGGCCCTG TTTATTCAGG CAGTAGGAAC GACCAGCTGC 
ACCGTCGTCC TCTGGGACTT GGAGACCCAG AGCATGCAGT GTTTTTCTCT CAGCCAGAAG 
TGTACTCCTG TAGACATCAG CGGAGACCAG CAGCTGTGCC TTGCTTTGAC AGAGAACGGC 
CTGTCTCTGA TTTTGTTTGG TTTAACTCAA GAGGAGTTTC TGAACAGACT GATGATCCAT 
GGCAGTGCCA GCACTGTGGA CTCCCTTTGT CATCTCAACG GTTGGGGACG GTGCTCAATC 
CCCATCCACG CTCTAGAGGC TGGCATAGAA AATCGTCAGC TGGACACGGT AGATTTCTTC 
CTGAAGAGCA AGGAAAATCT TCTTACTCCA TCTTCAAAAT CTCCTGCCCC TGATCAGCAG 
CAGCACTTCT CCTCCCAGCT CTACCTAAGG CAGGTGGAAG AGATGACCCC AGCCCTGGAC 
TTACTCTGCT CAGCAATTAG AGAAAGCGAC TCCGAAACCC AAAGCAAGCA CTTTGCAGAG 
CAGCTGCTTC ACCTCACACT GTCTTTCCTT AACAAGCAAA TGAGGGAGCT TTTTGATCAC 
ACTGAAGAGC TAGATGAGCA TTTACAGAAA GGAGCAGCCA TTTTGACCAG CTACATCAAT 
GAACTTCGGA CTTTTATGAT AAAGTTTCCT TGGAAGCCAG GAGATGCCAT AGATGAATCT 
GATGAAAATG AAGAGGTCCT CACAGTGAAA GAAGGCCAGG TTTGGGAGGA ACTCCGCTCT 
GAGGAAGTTA TTGCTGATGC CATTTTAAAT AACAGAATAC CAGAGGCACA AACTTTCTTC 
AGGATTACTG GTCATTCCGC TCAACGACTT GAGGAACTGG TTAGGATAGG CCTAGATTTG 
GCATTTGACA GTTTAAAAAA GAACAGCGTG GAGGAGGCCT CCAGGCTCTT GAGGAACATG 
GGCTTCAGTG TGGAAGACGA GCTGCTTAAG ATAAGCTTTT ATACGACTGA TAAAAACATA 
CGGGACTTTT TGGTTGAAAT TTTAAAAGAA AAGGAATGTT TTTCTGAAAA AGAAAGAAGA 
ACTGTGGACT TTGTCCATCA AGCTGAAAGG CTTTACTCAG GACACTTCCA GGAAAATGCG 
CCGATGCAGG CTTTTCCCAG GTATTGGATA AAGGAACAGG ACTGTTTCAA ACACAAATCT 
GTCTTGGACA CGTTCCTCAA ATGTGATACA AAAGATGAGT TTACCAAACA GGACCATAGA 
ATTGCACTGA ATTGGGCTCA CTGGTGGGAT CGGCAAACAC AGGAGTGTAT CCTTCTCCCC 
AGAATAAGTT CAGAAGAATA TAAAGCACAT TCCCCAGAAG CCCTGTGGAG ACACCTCACA 
GCTCGCCATG ACTGGTCAAG CATCAGCTTG TGGATGGAAG AATTTCAGGC CCAGGAAACT 
GTGTCACCTC AGCAGAGCAA GTGGCCTCCT CTGAGTGCTG CCGTCATCGA GCAGAACACT 
TGCTGCAGCA GGCACATGAG GAACGCGATC TTAGACCAGC TGGCCAGATG TGGCATTTTT 
CTTGCATCTG AGCTGGAAGA CTTTGAACTT CTCCTCCTGA GACTGAGCCG GATTGGGGGT 
GTAATGCAGG ATGCCCTCCC CGTGCAGAGC TACAGATCCT CGGCAGGGTG CAACTTTGAT 
TCCCAAGTCA TCCTCTACTG TTTAGAGCAC GGCCTGCAGC ATCTTCTCTA CGTCTACCTT 
GACTATTACA AACTTAGCCC TGGAAATTGT CCCTTTTTGG AAAAGAAAGA ACTACATGAA 
GCTCACCCTT GGCTTGAATT TTTGGTTCAG TGTCGGCAAG TTTCCAGTAA CTTGACAGAT 
CCCAAACTGA TCTTTCAGGC CAGTCTTGCA AATGCTCAGA TTTCGATTCC CACCAATCAA 
GCCAGTGTGA GCAGCATGCT GTTGGAAGGA CACACCCTCT TGGCTCTTGC TACCACCATG 
TATGCTCCTG GGGGCGTCAG CCAGGTCATT CAGCATGAAG ACAGCGAGAA CTGTTTGAAG 
AAAGTGGACC CCCAGCTACT GAAGATGGCC TTAACGCCTT ACCCAAAGCT CAAAGCCGCG 
CTCTTCCCTC AGTGCACTGC TCCTAGTATT CTGCCCTCTG ACATTACACT CTACCACCTC 
ATCCAGTCCT TACCACCCTT TGATCCCAGC AGACTGTTTG GCTGGCAGTC TGCCAACACT 
CTAGCTATCG GAGATGCGGC CGGTCAGCTC CCACACTTCT CCAGTCCTGA CCTGGTGAGT 
AAATACGCCG TGGTGGAGCA GCTGAGCTAC ACGTATTACT TACATCACGG ACGCCCATCC 
TTCGCCTTCG GCACCTTTCT GGTCCAGGAG TTAATCAAGA GTAAGACTCC CAAGCAGCTG 
ATCCAGCAAG TAGGCAAGGA GGCCTATACC TTAGGGCTCT CCTCCTTCCA CAACCCTTCG 
GTAGGGGCCG CCTGTGTCTG CTTCTTAGAA TTGCTTGGCC TCAGCAGCCT CAAGCTCAGA 
GTTGACCTGA AAATGGCAAA CGTAGTTCTG GGCTCCAGGC GTAGAAATGA AGATGCACAT 
CCCAGCTTCA TCAGAGAGTC TCTGGCTGAG AAGCTGTCTA AGCTTGCTGA CGGTGACAGG 
GCAACCACAG AAGAGCTGCT CGTTCTCTTG GAGGAAGGCG TGTGGGACAG CATTGAGCAG 
CAGGGCTTTA ACAGGTTGTC CAGCGAATCC AGCAGCCAGT GGTCATTAGT GTTACAGTTC 
TGCATGCTCC ATGATATGAA ACTGAGCGTG TCCTACCTTA GAGAATGTGC CAAAGCAAAT 
GATTGGCTGC AGTTTCTGGT TCACAGCCAG CTCCATAACT ACCACCCAGA AGAGGTGGAA 
ACCCTGCTTC AGTATTTCAG TCCTGTCCTT CAGAGTCACT TGAAGCTGGC TTCTGAGAAA 
CTGTCATCAG GGTCCATCTC TAAGGACGAC AGCTGCCTCC AGGAACTCCA GAAAAACAAA 
GAAACAAGTG ATTTCTTTGA AATTCTCCAC CGGTGCTCGG ATGAGTCAGC TTCCTGGTGC 
TGGCTCCTGG CTGAAGCAGT GAGACATCGG GTTCCCATCC TCAGTGTCCT GGCCTCGTGT 
ATCCAGGGTG CCAGTGCTGT CCCCTGTCTC TGTGTTTGGA TTGTCACCTC TGTGGAGGAC 
AAGGTTGCAG CTGAAGCAAT AGAACACATT CAGATCTCTG TAGAGGACCA TCATTGGAGC 
CTCGAGGACC TGTCCATTAT CTGGAGAACT GTATTGACAA GACGGAAGAT CCACACTCTC 
ATCCGAGGCT TCCGGCTTTT CATTAAGGAT TCCCCACTTC TCTTGATTAT GGAAATGTAT 
GAACTGTGTG TGTTCTTCAA GAACTACGGA AAAGCCAAAG TTAAGCTTGT GGAGTTCCAG 
AAAAGCCTTG AAATGCTTGA CACAGTGGCT ACTCGGGTCC TTCCTGTCAT ACCTGCCACG 
TGGATGAGGG AGCAGGTGTG CTTCCTTTTG AAACTTATGC CACAGCAGTG CGAGACACAG 
TATGAGCTGC AGAAAGTCCT CCAACTGTTT GTCGGGATGG AGCAGCTCTT CTCCGATGGG 
CCAGACGTGC AGAAGCTGTA TCGGCTCAGC CAAGTACTGA AGGACACACC TATCACCATC 
AGCCCTGCAG TCATTTCCAG CTACAGCACT GAGAGTTTGC AGCGTGAGTG TAGATCTATT 
TTGGAGAAGC TAAAGGCAGA TGGACAGTTT GCTGTGGCCA GGAGAGTGGC AGAATTAGCA 
TCCCTGCCTG TGGACGGCTT GGTGATTGAA CAGTTAACGC AGGAAATGCA AATGCTAACA 
CACACCCAGC AGTGGTCCCT GAAACAAGCA AGACTTGACT TCTGGAGAAA ATGCCATGAA 
AATTTTAAGA AAAACTCTGT TTCAAACAGA GCAGCCTCTT CCTTCTTCTC CAGCCAAGCC 
CCGGAGGCGA GTGAGCACCC TGCTGAGCAG GGCAGCCTTG AGGAGCGCCA TCTGCTGCTC 
ACTCTGGCGG GCCACTGGCT TGCCCAAGAA GAGCCAGTTC CCGTGGAGGA ACTGGAGGAG 
CTGGAGAAAC AGATTTGGCT CTGCCGCATC GCCCAGCACA CATGTGGAGG GGCTGAAGAA 
GAAACCAAGC CCAGCCTCTC GCAACACAAG TTGGCCCCTG CTGAACTTTC TTCTGATAGC 
TCAGCCAGTG AGTTCTCCTT CTCCAAGCTT GCTGCTTTGA ACACATCCAA ATACTTAGAC 
CTTAATGGTC TTCCATCCAA GGAGATGTGT GAGAACACAC TGGAACACAG AGAGCAGGAG 
TCACTGAACA CTTTGATTGG GCAGCTGCTG GATGGTGGCT GTGTGCATGA AGCGAGCAGA 
GTGTGCCGGT ATTTCCGTGT CTATAGTCAG GATCTAGTGC TGCTGCTGCA CTGCAGAGCC 
TTGGCCTCCG CAGAGGCCAG CATGGAAGAT CTGCACTCAG AGATCCGTGC CCTCCTCTCA 
AATGCCACAC TACCTGAGGA CCTGGAGAGC CCTGGCGTCC CTCTTCGGAA AGTCCATAGC 
AGAGAAAAGC AGCCTTTCAA CCTAGCAGAG GAAAGCCAGA CATTTCTTCA GCTAACTGCT 
TTGTGTCAAG ACCGTACATT GGTAGGCATG AAGTTGTTGG ACAAGATTCC ATCTGTCCCC 
CATGGGGAGC TGTCATGCAC CACAGAGCTC CTGATCCTGG CCCATCACTG CTTTACCTGT 
GCGTGTTACA TGGAGGGCAT CATCAGAGTC CTACAGGCTG CCCGGATGCT TACAGATAAC 
CACTTGGCTC CCAATGAGGA GTATGGATTG GTGGTACGCC TCCTCACTGG CATTGGACGG 
TACAATGAGA TGACGTACAT ATTCGACTTG CTGCATCAAA AGCATTATTT TGAAGTTCTG 
ATGAGGAAGA AGCTAGATCC AACCGGCACC CTGAAAACAG CCCTACTAGA CTACATCAAA 
CGCTGCCACC CTGGAGACAG TGAAAAGCAC AATATGATCG CCCTGTGCTT CAGCATGTGT 
CGGGAGATAG GAGAGAACCA CGAGGCCGCA GCCTGCATCC AACTGAAGCT GATCGAATCT 
CAGCCCTGGG AGGAAAGTCT CAAGGATGGA GCCCAACTAA AACAGCTCCT GCTTAAAGCC 
CTGACTTTGA TGCTGGACGC AGCAGAGAGT TACGCAAAGG ACTCCTGTGT GCGGCAGGCC 
CTGCATTGTA ACCGGCTCAC CAAGTTGATA ACACTACAGA TTCACTTTCT CAACAGTGGC 
CAGAACACAA TGCTCATCAA CTTGGGCCAT CAGAAGCTAA TGGACTGTAT CATGACCCTA 
CCTCGGTTCT ACCAGGCTTC TATTGTAGCT GAGGCCTATG ACTTTGTTCC TGATTGGGCT 
GAAGTACTCT ACCAACAAGT GATTCTTAAA GGAGACTTTA GTTACTTGGA AGAATTTAAG 
CAGCAGAAGT TACTAAAACC CAATATATTT GAAGACATTT CCAAAAAATA TAAACAACAC 
CAGCCTACTG ACAGGGTCAC AGAAAACCTG AAGAAGTTAC TTTCATATTG TGAAGACATC 
TACCTGTATT ACAAGTTGGC ATATGAACAC AAGTTTTTTG AAATTGTAAA TATGCTTCTG 
AAGGATCCTC AAACAGGATG CTGTCTTAAA GATATGCTCG CGGGTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001178500.1
CDS	1..6948
Translation	MAAKPGPGSAASPGCAGAMERVLPMLLVRIPAEETAQLGPRAQLHRELEALGSLTAAGSLQVLSLAPGSRGGSSCRLQGPFRQFLWEESQNSTSADKPRVLVAREDCGLLIYEFDLRDGRCDAALLHSCCGRTLQKLTEDQGVSISFKSLRILSFDNSTSLLLINECLVVRVVFPGRESGIGVLGCLSLPLPAQAVDTIIDVQLCKRFLFLLSTLGWIHIFDTVKGMHVARVDLALPREDSEQQEPASISSFMSLKVSRDLDVLLLVSSSSTAVALNLHLYFRLHPGHLLCEGTLQDLPIEGPTGIDEDDLVNSAHNMKLTKVSFQVDRSWRAQLASLNESVRSSEPELSCYPPWFQGALCLESLESGNHSPIVPNHVFIPGDVPSGQYAFPQKEHSKTSEPRPWRTMHLSEQEQPTELTCLSVTGFTALFIQAVGTTSCTVVLWDLETQSMQCFSLSQKCTPVDISGDQQLCLALTENGLSLILFGLTQEEFLNRLMIHGSASTVDSLCHLNGWGRCSIPIHALEAGIENRQLDTVDFFLKSKENLLTPSSKSPAPDQQQHFSSQLYLRQVEEMTPALDLLCSAIRESDSETQSKHFAEQLLHLTLSFLNKQMRELFDHTEELDEHLQKGAAILTSYINELRTFMIKFPWKPGDAIDESDENEEVLTVKEGQVWEELRSEEVIADAILNNRIPEAQTFFRITGHSAQRLEELVRIGLDLAFDSLKKNSVEEASRLLRNMGFSVEDELLKISFYTTDKNIRDFLVEILKEKECFSEKERRTVDFVHQAERLYSGHFQENAPMQAFPRYWIKEQDCFKHKSVLDTFLKCDTKDEFTKQDHRIALNWAHWWDRQTQECILLPRISSEEYKAHSPEALWRHLTARHDWSSISLWMEEFQAQETVSPQQSKWPPLSAAVIEQNTCCSRHMRNAILDQLARCGIFLASELEDFELLLLRLSRIGGVMQDALPVQSYRSSAGCNFDSQVILYCLEHGLQHLLYVYLDYYKLSPGNCPFLEKKELHEAHPWLEFLVQCRQVSSNLTDPKLIFQASLANAQISIPTNQASVSSMLLEGHTLLALATTMYAPGGVSQVIQHEDSENCLKKVDPQLLKMALTPYPKLKAALFPQCTAPSILPSDITLYHLIQSLPPFDPSRLFGWQSANTLAIGDAAGQLPHFSSPDLVSKYAVVEQLSYTYYLHHGRPSFAFGTFLVQELIKSKTPKQLIQQVGKEAYTLGLSSFHNPSVGAACVCFLELLGLSSLKLRVDLKMANVVLGSRRRNEDAHPSFIRESLAEKLSKLADGDRATTEELLVLLEEGVWDSIEQQGFNRLSSESSSQWSLVLQFCMLHDMKLSVSYLRECAKANDWLQFLVHSQLHNYHPEEVETLLQYFSPVLQSHLKLASEKLSSGSISKDDSCLQELQKNKETSDFFEILHRCSDESASWCWLLAEAVRHRVPILSVLASCIQGASAVPCLCVWIVTSVEDKVAAEAIEHIQISVEDHHWSLEDLSIIWRTVLTRRKIHTLIRGFRLFIKDSPLLLIMEMYELCVFFKNYGKAKVKLVEFQKSLEMLDTVATRVLPVIPATWMREQVCFLLKLMPQQCETQYELQKVLQLFVGMEQLFSDGPDVQKLYRLSQVLKDTPITISPAVISSYSTESLQRECRSILEKLKADGQFAVARRVAELASLPVDGLVIEQLTQEMQMLTHTQQWSLKQARLDFWRKCHENFKKNSVSNRAASSFFSSQAPEASEHPAEQGSLEERHLLLTLAGHWLAQEEPVPVEELEELEKQIWLCRIAQHTCGGAEEETKPSLSQHKLAPAELSSDSSASEFSFSKLAALNTSKYLDLNGLPSKEMCENTLEHREQESLNTLIGQLLDGGCVHEASRVCRYFRVYSQDLVLLLHCRALASAEASMEDLHSEIRALLSNATLPEDLESPGVPLRKVHSREKQPFNLAEESQTFLQLTALCQDRTLVGMKLLDKIPSVPHGELSCTTELLILAHHCFTCACYMEGIIRVLQAARMLTDNHLAPNEEYGLVVRLLTGIGRYNEMTYIFDLLHQKHYFEVLMRKKLDPTGTLKTALLDYIKRCHPGDSEKHNMIALCFSMCREIGENHEAAACIQLKLIESQPWEESLKDGAQLKQLLLKALTLMLDAAESYAKDSCVRQALHCNRLTKLITLQIHFLNSGQNTMLINLGHQKLMDCIMTLPRFYQASIVAEAYDFVPDWAEVLYQQVILKGDFSYLEEFKQQKLLKPNIFEDISKKYKQHQPTDRVTENLKKLLSYCEDIYLYYKLAYEHKFFEIVNMLLKDPQTGCCLKDMLAG

Target ORF information:

RefSeq Version	NM_001191571.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus SPG11 vesicle trafficking associated, spatacsin (Spg11), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001191571.1

ORF Insert Sequence:

ATGGCTGCCA AGCCAGGGCC CGGAAGTGCT GCCTCCCCCG GCTGTGCCGG CGCCATGGAG 
CGAGTTCTAC CCATGCTGCT GGTGCGGATC CCTGCCGAGG AGACGGCGCA GCTGGGACCG 
CGGGCGCAGC TGCACCGAGA GCTGGAGGCC CTGGGGAGCC TGACGGCTGC GGGCAGTCTC 
CAAGTGCTTT CCTTGGCGCC GGGCAGTAGG GGTGGAAGCA GCTGCCGCCT CCAAGGCCCC 
TTTAGGCAGT TCCTGTGGGA GGAGTCTCAG AACAGCACAT CAGCAGACAA GCCCAGAGTG 
CTTGTTGCTC GAGAAGATTG TGGGCTGCTC ATATATGAAT TTGACTTGAG AGATGGAAGA 
TGTGACGCAG CCTTACTACA CAGCTGTTGT GGGAGGACCC TGCAGAAGCT CACTGAAGAT 
CAAGGTGTCA GCATTTCCTT CAAGTCTTTG AGGATTCTGT CATTTGACAA CAGCACGTCC 
TTACTGCTCA TCAATGAGTG TCTCGTCGTA CGTGTCGTGT TTCCGGGGAG AGAATCTGGG 
ATTGGAGTGC TGGGCTGCCT CTCACTGCCC TTGCCTGCAC AGGCAGTGGA CACGATAATT 
GACGTGCAGC TCTGCAAGAG GTTTCTTTTC CTTCTGAGTA CATTAGGCTG GATCCATATT 
TTTGACACTG TAAAGGGCAT GCATGTAGCC CGCGTGGACT TGGCTCTTCC CCGAGAAGAT 
TCTGAGCAGC AGGAGCCTGC CAGCATTTCT TCCTTTATGT CACTGAAAGT GTCTCGGGAC 
TTAGATGTTT TGCTACTCGT CAGCTCGTCC AGTACTGCAG TTGCTCTTAA CCTTCACTTG 
TATTTCAGGC TACACCCAGG GCACCTGCTG TGTGAAGGAA CTCTGCAAGA CCTCCCTATT 
GAAGGGCCTA CAGGAATAGA TGAAGATGAC CTTGTTAATT CTGCCCACAA CATGAAACTG 
ACCAAGGTGT CCTTCCAAGT TGATAGGTCC TGGAGAGCCC AGCTGGCATC ACTGAATGAA 
TCTGTGAGGA GCTCAGAACC AGAGCTTTCC TGCTATCCTC CGTGGTTCCA GGGTGCTTTG 
TGTTTGGAGT CCCTGGAATC TGGTAACCAC TCCCCCATTG TGCCAAACCA CGTTTTCATT 
CCAGGGGATG TCCCGAGTGG CCAGTATGCT TTTCCTCAGA AGGAGCACAG CAAGACCAGT 
GAGCCAAGGC CGTGGAGGAC AATGCACCTG AGTGAACAAG AGCAGCCCAC AGAGCTCACG 
TGTCTGTCTG TGACGGGATT TACGGCCCTG TTTATTCAGG CAGTAGGAAC GACCAGCTGC 
ACCGTCGTCC TCTGGGACTT GGAGACCCAG AGCATGCAGT GTTTTTCTCT CAGCCAGAAG 
TGTACTCCTG TAGACATCAG CGGAGACCAG CAGCTGTGCC TTGCTTTGAC AGAGAACGGC 
CTGTCTCTGA TTTTGTTTGG TTTAACTCAA GAGGAGTTTC TGAACAGACT GATGATCCAT 
GGCAGTGCCA GCACTGTGGA CTCCCTTTGT CATCTCAACG GTTGGGGACG GTGCTCAATC 
CCCATCCACG CTCTAGAGGC TGGCATAGAA AATCGTCAGC TGGACACGGT AGATTTCTTC 
CTGAAGAGCA AGGAAAATCT TCTTACTCCA TCTTCAAAAT CTCCTGCCCC TGATCAGCAG 
CAGCACTTCT CCTCCCAGCT CTACCTAAGG CAGGTGGAAG AGATGACCCC AGCCCTGGAC 
TTACTCTGCT CAGCAATTAG AGAAAGCGAC TCCGAAACCC AAAGCAAGCA CTTTGCAGAG 
CAGCTGCTTC ACCTCACACT GTCTTTCCTT AACAAGCAAA TGAGGGAGCT TTTTGATCAC 
ACTGAAGAGC TAGATGAGCA TTTACAGAAA GGAGCAGCCA TTTTGACCAG CTACATCAAT 
GAACTTCGGA CTTTTATGAT AAAGTTTCCT TGGAAGCCAG GAGATGCCAT AGATGAATCT 
GATGAAAATG AAGAGGTCCT CACAGTGAAA GAAGGCCAGG TTTGGGAGGA ACTCCGCTCT 
GAGGAAGTTA TTGCTGATGC CATTTTAAAT AACAGAATAC CAGAGGCACA AACTTTCTTC 
AGGATTACTG GTCATTCCGC TCAACGACTT GAGGAACTGG TTAGGATAGG CCTAGATTTG 
GCATTTGACA GTTTAAAAAA GAACAGCGTG GAGGAGGCCT CCAGGCTCTT GAGGAACATG 
GGCTTCAGTG TGGAAGACGA GCTGCTTAAG ATAAGCTTTT ATACGACTGA TAAAAACATA 
CGGGACTTTT TGGTTGAAAT TTTAAAAGAA AAGGAATGTT TTTCTGAAAA AGAAAGAAGA 
ACTGTGGACT TTGTCCATCA AGCTGAAAGG CTTTACTCAG GACACTTCCA GGAAAATGCG 
CCGATGCAGG CTTTTCCCAG GTATTGGATA AAGGAACAGG ACTGTTTCAA ACACAAATCT 
GTCTTGGACA CGTTCCTCAA ATGTGATACA AAAGATGAGT TTACCAAACA GGACCATAGA 
ATTGCACTGA ATTGGGCTCA CTGGTGGGAT CGGCAAACAC AGGAGTGTAT CCTTCTCCCC 
AGAATAAGTT CAGAAGAATA TAAAGCACAT TCCCCAGAAG CCCTGTGGAG ACACCTCACA 
GCTCGCCATG ACTGGTCAAG CATCAGCTTG TGGATGGAAG AATTTCAGGC CCAGGAAACT 
GTGTCACCTC AGCAGAGCAA GTGGCCTCCT CTGAGTGCTG CCGTCATCGA GCAGAACACT 
TGCTGCAGCA GGCACATGAG GAACGCGATC TTAGACCAGC TGGCCAGATG TGGCATTTTT 
CTTGCATCTG AGCTGGAAGA CTTTGAACTT CTCCTCCTGA GACTGAGCCG GATTGGGGGT 
GTAATGCAGG ATGCCCTCCC CGTGCAGAGC TACAGATCCT CGGCAGGGTG CAACTTTGAT 
TCCCAAGTCA TCCTCTACTG TTTAGAGCAC GGCCTGCAGC ATCTTCTCTA CGTCTACCTT 
GACTATTACA AACTTAGCCC TGGAAATTGT CCCTTTTTGG AAAAGAAAGA ACTACATGAA 
GCTCACCCTT GGCTTGAATT TTTGGTTCAG TGTCGGCAAG TTTCCAGTAA CTTGACAGAT 
CCCAAACTGA TCTTTCAGGC CAGTCTTGCA AATGCTCAGA TTTCGATTCC CACCAATCAA 
GCCAGTGTGA GCAGCATGCT GTTGGAAGGA CACACCCTCT TGGCTCTTGC TACCACCATG 
TATGCTCCTG GGGGCGTCAG CCAGGTCATT CAGCATGAAG ACAGCGAGAA CTGTTTGAAG 
AAAGTGGACC CCCAGCTACT GAAGATGGCC TTAACGCCTT ACCCAAAGCT CAAAGCCGCG 
CTCTTCCCTC AGTGCACTGC TCCTAGTATT CTGCCCTCTG ACATTACACT CTACCACCTC 
ATCCAGTCCT TACCACCCTT TGATCCCAGC AGACTGTTTG GCTGGCAGTC TGCCAACACT 
CTAGCTATCG GAGATGCGGC CGGTCAGCTC CCACACTTCT CCAGTCCTGA CCTGGTGAGT 
AAATACGCCG TGGTGGAGCA GCTGAGCTAC ACGTATTACT TACATCACGG ACGCCCATCC 
TTCGCCTTCG GCACCTTTCT GGTCCAGGAG TTAATCAAGA GTAAGACTCC CAAGCAGCTG 
ATCCAGCAAG TAGGCAAGGA GGCCTATACC TTAGGGCTCT CCTCCTTCCA CAACCCTTCG 
GTAGGGGCCG CCTGTGTCTG CTTCTTAGAA TTGCTTGGCC TCAGCAGCCT CAAGCTCAGA 
GTTGACCTGA AAATGGCAAA CGTAGTTCTG GGCTCCAGGC GTAGAAATGA AGATGCACAT 
CCCAGCTTCA TCAGAGAGTC TCTGGCTGAG AAGCTGTCTA AGCTTGCTGA CGGTGACAGG 
GCAACCACAG AAGAGCTGCT CGTTCTCTTG GAGGAAGGCG TGTGGGACAG CATTGAGCAG 
CAGGGCTTTA ACAGGTTGTC CAGCGAATCC AGCAGCCAGT GGTCATTAGT GTTACAGTTC 
TGCATGCTCC ATGATATGAA ACTGAGCGTG TCCTACCTTA GAGAATGTGC CAAAGCAAAT 
GATTGGCTGC AGTTTCTGGT TCACAGCCAG CTCCATAACT ACCACCCAGA AGAGGTGGAA 
ACCCTGCTTC AGTATTTCAG TCCTGTCCTT CAGAGTCACT TGAAGCTGGC TTCTGAGAAA 
CTGTCATCAG GGTCCATCTC TAAGGACGAC AGCTGCCTCC AGGAACTCCA GAAAAACAAA 
GAAACAAGTG ATTTCTTTGA AATTCTCCAC CGGTGCTCGG ATGAGTCAGC TTCCTGGTGC 
TGGCTCCTGG CTGAAGCAGT GAGACATCGG GTTCCCATCC TCAGTGTCCT GGCCTCGTGT 
ATCCAGGGTG CCAGTGCTGT CCCCTGTCTC TGTGTTTGGA TTGTCACCTC TGTGGAGGAC 
AAGGTTGCAG CTGAAGCAAT AGAACACATT CAGATCTCTG TAGAGGACCA TCATTGGAGC 
CTCGAGGACC TGTCCATTAT CTGGAGAACT GTATTGACAA GACGGAAGAT CCACACTCTC 
ATCCGAGGCT TCCGGCTTTT CATTAAGGAT TCCCCACTTC TCTTGATTAT GGAAATGTAT 
GAACTGTGTG TGTTCTTCAA GAACTACGGA AAAGCCAAAG TTAAGCTTGT GGAGTTCCAG 
AAAAGCCTTG AAATGCTTGA CACAGTGGCT ACTCGGGTCC TTCCTGTCAT ACCTGCCACG 
TGGATGAGGG AGCAGGTGTG CTTCCTTTTG AAACTTATGC CACAGCAGTG CGAGACACAG 
TATGAGCTGC AGAAAGTCCT CCAACTGTTT GTCGGGATGG AGCAGCTCTT CTCCGATGGG 
CCAGACGTGC AGAAGCTGTA TCGGCTCAGC CAAGTACTGA AGGACACACC TATCACCATC 
AGCCCTGCAG TCATTTCCAG CTACAGCACT GAGAGTTTGC AGCGTGAGTG TAGATCTATT 
TTGGAGAAGC TAAAGGCAGA TGGACAGTTT GCTGTGGCCA GGAGAGTGGC AGAATTAGCA 
TCCCTGCCTG TGGACGGCTT GGTGATTGAA CAGTTAACGC AGGAAATGCA AATGCTAACA 
CACACCCAGC AGTGGTCCCT GAAACAAGCA AGACTTGACT TCTGGAGAAA ATGCCATGAA 
AATTTTAAGA AAAACTCTGT TTCAAACAGA GCAGCCTCTT CCTTCTTCTC CAGCCAAGCC 
CCGGAGGCGA GTGAGCACCC TGCTGAGCAG GGCAGCCTTG AGGAGCGCCA TCTGCTGCTC 
ACTCTGGCGG GCCACTGGCT TGCCCAAGAA GAGCCAGTTC CCGTGGAGGA ACTGGAGGAG 
CTGGAGAAAC AGATTTGGCT CTGCCGCATC GCCCAGCACA CATGTGGAGG GGCTGAAGAA 
GAAACCAAGC CCAGCCTCTC GCAACACAAG TTGGCCCCTG CTGAACTTTC TTCTGATAGC 
TCAGCCAGTG AGTTCTCCTT CTCCAAGCTT GCTGCTTTGA ACACATCCAA ATACTTAGAC 
CTTAATGGTC TTCCATCCAA GGAGATGTGT GAGAACACAC TGGAACACAG AGAGCAGGAG 
TCACTGAACA CTTTGATTGG GCAGCTGCTG GATGGTGGCT GTGTGCATGA AGCGAGCAGA 
GTGTGCCGGT ATTTCCGTGT CTATAGTCAG GATCTAGTGC TGCTGCTGCA CTGCAGAGCC 
TTGGCCTCCG CAGAGGCCAG CATGGAAGAT CTGCACTCAG AGATCCGTGC CCTCCTCTCA 
AATGCCACAC TACCTGAGGA CCTGGAGAGC CCTGGCGTCC CTCTTCGGAA AGTCCATAGC 
AGAGAAAAGC AGCCTTTCAA CCTAGCAGAG GAAAGCCAGA CATTTCTTCA GCTAACTGCT 
TTGTGTCAAG ACCGTACATT GGTAGGCATG AAGTTGTTGG ACAAGATTCC ATCTGTCCCC 
CATGGGGAGC TGTCATGCAC CACAGAGCTC CTGATCCTGG CCCATCACTG CTTTACCTGT 
GCGTGTTACA TGGAGGGCAT CATCAGAGTC CTACAGGCTG CCCGGATGCT TACAGATAAC 
CACTTGGCTC CCAATGAGGA GTATGGATTG GTGGTACGCC TCCTCACTGG CATTGGACGG 
TACAATGAGA TGACGTACAT ATTCGACTTG CTGCATCAAA AGCATTATTT TGAAGTTCTG 
ATGAGGAAGA AGCTAGATCC AACCGGCACC CTGAAAACAG CCCTACTAGA CTACATCAAA 
CGCTGCCACC CTGGAGACAG TGAAAAGCAC AATATGATCG CCCTGTGCTT CAGCATGTGT 
CGGGAGATAG GAGAGAACCA CGAGGCCGCA GCCTGCATCC AACTGAAGCT GATCGAATCT 
CAGCCCTGGG AGGAAAGTCT CAAGGATGGA GCCCAACTAA AACAGCTCCT GCTTAAAGCC 
CTGACTTTGA TGCTGGACGC AGCAGAGAGT TACGCAAAGG ACTCCTGTGT GCGGCAGGCC 
CTGCATTGTA ACCGGCTCAC CAAGTTGATA ACACTACAGA TTCACTTTCT CAACAGTGGC 
CAGAACACAA TGCTCATCAA CTTGGGCCAT CAGAAGCTAA TGGACTGTAT CATGACCCTA 
CCTCGGTTCT ACCAGGCTTC TATTGTAGCT GAGGCCTATG ACTTTGTTCC TGATTGGGCT 
GAAGTACTCT ACCAACAAGT GATTCTTAAA GGAGACTTTA GTTACTTGGA AGAATTTAAG 
CAGCAGAAGT TACTAAAACC CAATATATTT GAAGACATTT CCAAAAAATA TAAACAACAC 
CAGCCTACTG ACAGGGTCAC AGAAAACCTG AAGAAGTTAC TTTCATATTG TGAAGACATC 
TACCTGTATT ACAAGTTGGC ATATGAACAC AAGTTTTTTG AAATTGTAAA TATGCTTCTG 
AAGGATCCTC AAACAGGATG CTGTCTTAAA GATATGCTCG CGGGTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Spg11 ( XM_017591740.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_017447229.1 Rattus norvegicus spastic paraplegia 11 (autosomal recessive) (Spg11), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ORa60399
Clone ID Related Accession (Same CDS sequence)	XM_017591740.1
Accession Version	XM_017591740.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7305bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1469376000000
Organism	Rattus norvegicus(Norway rat)
Product	spatacsin isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005102.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTGCCA AGCCAGGGCC CGGAAGTGCT GCCTCCCCCG GCTGTGCCGG CGCCATGGAG 
CGAGTTCTAC CCATGCTGCT GGTGCGGATC CCTGCCGAGG AGACGGCGCA GCTGGGACCG 
CGGGCGCAGC TGCACCGAGA GCTGGAGGCC CTGGGGAGCC TGACGGCTGC GGGCAGTCTC 
CAAGTGCTTT CCTTGGCGCC GGGCAGTAGG GGTGGAAGCA GCTGCCGCCT CCAAGGCCCC 
TTTAGGCAGT TCCTGTGGGA GGAGTCTCAG AACAGCACAT CAGCAGACAA GCCCAGAGTG 
CTTGTTGCTC GAGAAGATTG TGGGCTGCTC ATATATGAAT TTGACTTGAG AGATGGAAGA 
TGTGACGCAG CCTTACTACA CAGCTGTTGT GGGAGGACCC TGCAGAAGCT CACTGAAGAT 
CAAGGTGTCA GCATTTCCTT CAAGTCTTTG AGGATTCTGT CATTTGACAA CAGCACGTCC 
TTACTGCTCA TCAATGAGTG TCTCGTCGTA CGTGTCGTGT TTCCGGGGAG AGAATCTGGG 
ATTGGAGTGC TGGGCTGCCT CTCACTGCCC TTGCCTGCAC AGGCAGTGGA CACGATAATT 
GACGTGCAGC TCTGCAAGAG GTTTCTTTTC CTTCTGAGTA CATTAGGCTG GATCCATATT 
TTTGACACTG TAAAGGGCAT GCATGTAGCC CGCGTGGACT TGGCTCTTCC CCGAGAAGAT 
TCTGAGCAGC AGGAGCCTGC CAGCATTTCT TCCTTTATGT CACTGAAAGT GTCTCGGGAC 
TTAGATGTTT TGCTACTCGT CAGCTCGTCC AGTACTGCAG TTGCTCTTAA CCTTCACTTG 
TATTTCAGGC TACACCCAGG GCACCTGCTG TGTGAAGGAA CTCTGCAAGA CCTCCCTATT 
GAAGGGCCTA CAGGAATAGA TGAAGATGAC CTTGTTAATT CTGCCCACAA CATGAAACTG 
ACCAAGGTGT CCTTCCAAGT TGATAGGTCC TGGAGAGCCC AGCTGGCATC ACTGAATGAA 
TCTGTGAGGA GCTCAGAACC AGAGCTTTCC TGCTATCCTC CGTGGTTCCA GGGTGCTTTG 
TGTTTGGAGT CCCTGGAATC TGGTAACCAC TCCCCCATTG TGCCAAACCA CGTTTTCATT 
CCAGGGGATG TCCCGAGTGG CCAGTATGCT TTTCCTCAGA AGGAGCACAG CAAGACCAGT 
GAGCCAAGGC CGTGGAGGAC AATGCACCTG AGTGAACAAG AGCAGCCCAC AGAGCTCACG 
TGTCTGTCTG TGACGGGATT TACGGCCCTG TTTATTCAGG CAGTAGGAAC GACCAGCTGC 
ACCGTCGTCC TCTGGGACTT GGAGACCCAG AGCATGCAGT GTTTTTCTCT CAGCCAGAAG 
TGTACTCCTG TAGACATCAG CGGAGACCAG CAGCTGTGCC TTGCTTTGAC AGAGAACGGC 
CTGTCTCTGA TTTTGTTTGG TTTAACTCAA GAGGAGTTTC TGAACAGACT GATGATCCAT 
GGCAGTGCCA GCACTGTGGA CTCCCTTTGT CATCTCAACG GTTGGGGACG GTGCTCAATC 
CCCATCCACG CTCTAGAGGC TGGCATAGAA AATCGTCAGC TGGACACGGT AGATTTCTTC 
CTGAAGAGCA AGGAAAATCT TCTTACTCCA TCTTCAAAAT CTCCTGCCCC TGATCAGCAG 
CAGCACTTCT CCTCCCAGCT CTACCTAAGG CAGGTGGAAG AGATGACCCC AGCCCTGGAC 
TTACTCTGCT CAGCAATTAG AGAAAGCGAC TCCGAAACCC AAAGCAAGCA CTTTGCAGAG 
CAGCTGCTTC ACCTCACACT GTCTTTCCTT AACAAGCAAA TGAGGGAGCT TTTTGATCAC 
ACTGAAGAGC TAGATGAGCA TTTACAGAAA GGAGCAGCCA TTTTGACCAG CTACATCAAT 
GAACTTCGGA CTTTTATGAT AAAGTTTCCT TGGAAGCCAG GAGATGCCAT AGATGAATCT 
GATGAAAATG AAGAGGTCCT CACAGTGAAA GAAGGCCAGG TTTGGGAGGA ACTCCGCTCT 
GAGGAAGTTA TTGCTGATGC CATTTTAAAT AACAGAATAC CAGAGGCACA AACTTTCTTC 
AGGATTACTG GTCATTCCGC TCAACGACTT GAGGAACTGG TTAGGATAGG CCTAGATTTG 
GCATTTGACA GTTTAAAAAA GAACAGCGTG GAGGAGGCCT CCAGGCTCTT GAGGAACATG 
GGCTTCAGTG TGGAAGACGA GCTGCTTAAG ATAAGCTTTT ATACGACTGA TAAAAACATA 
CGGGACTTTT TGGTTGAAAT TTTAAAAGAA AAGGAATGTT TTTCTGAAAA AGAAAGAAGA 
ACTGTGGACT TTGTCCATCA AGCTGAAAGG CTTTACTCAG GACACTTCCA GGAAAATGCG 
CCGATGCAGG CTTTTCCCAG GTATTGGATA AAGGAACAGG ACTGTTTCAA ACACAAATCT 
GTCTTGGACA CGTTCCTCAA ATGTGATACA AAAGATGAGT TTACCAAACA GGACCATAGA 
ATTGCACTGA ATTGGGCTCA CTGGTGGGAT CGGCAAACAC AGGAGTGTAT CCTTCTCCCC 
AGAATAAGTT CAGAAGAATA TAAAGCACAT TCCCCAGAAG CCCTGTGGAG ACACCTCACA 
GCTCGCCATG ACTGGTCAAG CATCAGCTTG TGGATGGAAG AATTTCAGGC CCAGGAAACT 
GTGTCACCTC AGCAGAGCAA GTGGCCTCCT CTGAGTGCTG CCGTCATCGA GCAGAACACT 
TGCTGCAGCA GGCACATGAG GAACGCGATC TTAGACCAGC TGGCCAGATG TGGCATTTTT 
CTTGCATCTG AGCTGGAAGA CTTTGAACTT CTCCTCCTGA GACTGAGCCG GATTGGGGGT 
GTAATGCAGG ATGCCCTCCC CGTGCAGAGC TACAGATCCT CGGCAGGGTG CAACTTTGAT 
TCCCAAGTCA TCCTCTACTG TTTAGAGCAC GGCCTGCAGC ATCTTCTCTA CGTCTACCTT 
GACTATTACA AACTTAGCCC TGGAAATTGT CCCTTTTTGG AAAAGAAAGA ACTACATGAA 
GCTCACCCTT GGCTTGAATT TTTGGTTCAG TGTCGGCAAG TTTCCAGTAA CTTGACAGAT 
CCCAAACTGA TCTTTCAGGC CAGTCTTGCA AATGCTCAGA TTTCGATTCC CACCAATCAA 
GCCAGTGTGA GCAGCATGCT GTTGGAAGGA CACACCCTCT TGGCTCTTGC TACCACCATG 
TATGCTCCTG GGGGCGTCAG CCAGGTCATT CAGCATGAAG ACAGCGAGAA CTGTTTGAAG 
AAAGTGGACC CCCAGCTACT GAAGATGGCC TTAACGCCTT ACCCAAAGCT CAAAGCCGCG 
CTCTTCCCTC AGTGCACTGC TCCTAGTATT CTGCCCTCTG ACATTACACT CTACCACCTC 
ATCCAGTCCT TACCACCCTT TGATCCCAGC AGACTGTTTG GCTGGCAGTC TGCCAACACT 
CTAGCTATCG GAGATGCGGC CGGTCAGCTC CCACACTTCT CCAGTCCTGA CCTGGTGAGT 
AAATACGCCG TGGTGGAGCA GCTGAGCTAC ACGTATTACT TACATCACGG ACGCCCATCC 
TTCGCCTTCG GCACCTTTCT GGTCCAGGAG TTAATCAAGA GTAAGACTCC CAAGCAGCTG 
ATCCAGCAAG TAGGCAAGGA GGCCTATACC TTAGGGCTCT CCTCCTTCCA CAACCCTTCG 
GTAGGGGCCG CCTGTGTCTG CTTCTTAGAA TTGCTTGGCC TCAGCAGCCT CAAGCTCAGA 
GTTGACCTGA AAATGGCAAA CGTAGTTCTG GGCTCCAGGC GTAGAAATGA AGATGCACAT 
CCCAGCTTCA TCAGAGAGTC TCTGGCTGAG AAGCTGTCTA AGCTTGCTGA CGGTGACAGG 
GCAACCACAG AAGAGCTGCT CGTTCTCTTG GAGGAAGGCG TGTGGGACAG CATTGAGCAG 
CAGGGCTTTA ACAGGTTGTC CAGCGAATCC AGCAGCCAGT GGTCATTAGT GTTACAGTTC 
TGCATGCTCC ATGATATGAA ACTGAGCGTG TCCTACCTTA GAGAATGTGC CAAAGCAAAT 
GATTGGCTGC AGTTTCTGGT TCACAGCCAG CTCCATAACT ACCACCCAGA AGAGGTGGAA 
ACCCTGCTTC AGTATTTCAG TCCTGTCCTT CAGAGTCACT TGAAGCTGGC TTCTGAGAAA 
CTGTCATCAG GGTCCATCTC TAAGGACGAC AGCTGCCTCC AGGAACTCCA GAAAAACAAA 
GAAACAAGTG ATTTCTTTGA AATTCTCCAC CGGTGCTCGG ATGAGTCAGC TTCCTGGTGC 
TGGCTCCTGG CTGAAGCAGT GAGACATCGG GTTCCCATCC TCAGTGTCCT GGCCTCGTGT 
ATCCAGGGTG CCAGTGCTGT CCCCTGTCTC TGTGTTTGGA TTGTCACCTC TGTGGAGGAC 
AAGGTTGCAG CTGAAGCAAT AGAACACATT CAGATCTCTG TAGAGGACCA TCATTGGAGC 
CTCGAGGACC TGTCCATTAT CTGGAGAACT GTATTGACAA GACGGAAGAT CCACACTCTC 
ATCCGAGGCT TCCGGCTTTT CATTAAGGAT TCCCCACTTC TCTTGATTAT GGAAATGTAT 
GAACTGTGTG TGTTCTTCAA GAACTACGGA AAAGCCAAAG TTAAGCTTGT GGAGTTCCAG 
AAAAGCCTTG AAATGCTTGA CACAGTGGCT ACTCGGGTCC TTCCTGTCAT ACCTGCCACG 
TGGATGAGGG AGCAGGTGTG CTTCCTTTTG AAACTTATGC CACAGCAGTG CGAGACACAG 
TATGAGCTGC AGAAAGTCCT CCAACTGTTT GTCGGGATGG AGCAGCTCTT CTCCGATGGG 
CCAGACGTGC AGAAGCTGTA TCGGCTCAGC CAAGTACTGA AGGACACACC TATCACCATC 
AGCCCTGCAG TCATTTCCAG CTACAGCACT GAGAGTTTGC AGCGTGAGTG TAGATCTATT 
TTGGAGAAGC TAAAGGCAGA TGGACAGTTT GCTGTGGCCA GGAGAGTGGC AGAATTAGCA 
TCCCTGCCTG TGGACGGCTT GGTGATTGAA CAGTTAACGC AGGAAATGCA AATGCTAACA 
CACACCCAGC AGTGGTCCCT GAAACAAGCA AGACTTGACT TCTGGAGAAA ATGCCATGAA 
AATTTTAAGA AAAACTCTGT TTCAAACAGA GCAGCCTCTT CCTTCTTCTC CAGCCAAGCC 
CCGGAGGCGA GTGAGCACCC TGCTGAGCAG GGCAGCCTTG AGGAGCGCCA TCTGCTGCTC 
ACTCTGGCGG GCCACTGGCT TGCCCAAGAA GAGCCAGTTC CCGTGGAGGA ACTGGAGGAG 
CTGGAGAAAC AGATTTGGCT CTGCCGCATC GCCCAGCACA CATGTGGAGG GGCTGAAGAA 
GAAACCAAGC CCAGCCTCTC GCAACACAAG TTGGCCCCTG CTGAACTTTC TTCTGATAGC 
TCAGCCAGTG AGTTCTCCTT CTCCAAGCTT GCTGCTTTGA ACACATCCAA ATACTTAGAC 
CTTAATGGTC TTCCATCCAA GGAGATGTGT GAGAACACAC TGGAACACAG AGAGCAGGAG 
TCACTGAACA CTTTGATTGG GCAGCTGCTG GATGGTGGCT GTGTGCATGA AGCGAGCAGA 
GTGTGCCGGT ATTTCCGTGT CTATAGTCAG GATCTAGTGC TGCTGCTGCA CTGCAGAGCC 
TTGGCCTCCG CAGAGGCCAG CATGGAAGAT CTGCACTCAG AGATCCGTGC CCTCCTCTCA 
AATGCCACAC TACCTGAGGA CCTGGAGAGC CCTGGCGTCC CTCTTCGGAA AGTCCATAGC 
AACATGTGTT TGCTTTCAGC TTCGAGTTTG GACAGTCTGT CATTTGCGAT GGTACCACCT 
ACTGATGAAG TGGCCAGGAG TCTCCAAACT CTCATAAGCA GGTGCCTCCA TGGGAAGGAT 
TACTGTCGGC AGGTCCTCTG TCTGTATGAA CTTGCCAAGG AGTTGGGCTG TTCCTATGGA 
GATGTTGCTA CCAGGGACAG TGAAGCCATG CTCCGAGCAA TCTTGGCTTC TCAGCGGCCT 
GACCGATGCA GACAAGCCCA GGTCTTCATC AACACCCAGG GCCTTGAACC AGACACTGTG 
GCTGAACTTG TGGCTGAAGA GGTGACACGA GAGCTACTGA CCCCATCAGA GGGAGCAGGA 
GAAAAGCAGC CTTTCAACCT AGCAGAGGAA AGCCAGACAT TTCTTCAGCT AACTGCTTTG 
TGTCAAGACC GTACATTGGT AGGCATGAAG TTGTTGGACA AGATTCCATC TGTCCCCCAT 
GGGGAGCTGT CATGCACCAC AGAGCTCCTG ATCCTGGCCC ATCACTGCTT TACCTGTGCG 
TGTTACATGG AGGGCATCAT CAGAGTCCTA CAGGCTGCCC GGATGCTTAC AGATAACCAC 
TTGGCTCCCA ATGAGGAGTA TGGATTGGTG GTACGCCTCC TCACTGGCAT TGGACGGTAC 
AATGAGATGA CGTACATATT CGACTTGCTG CATCAAAAGC ATTATTTTGA AGTTCTGATG 
AGGAAGAAGC TAGATCCAAC CGGCACCCTG AAAACAGCCC TACTAGACTA CATCAAACGC 
TGCCACCCTG GAGACAGTGA AAAGCACAAT ATGATCGCCC TGTGCTTCAG CATGTGTCGG 
GAGATAGGAG AGAACCACGA GGCCGCAGCC TGCATCCAAC TGAAGCTGAT CGAATCTCAG 
CCCTGGGAGG AAAGTCTCAA GGATGGAGCC CAACTAAAAC AGCTCCTGCT TAAAGCCCTG 
ACTTTGATGC TGGACGCAGC AGAGAGTTAC GCAAAGGACT CCTGTGTGCG GCAGGCCCTG 
CATTGTAACC GGCTCACCAA GTTGATAACA CTACAGATTC ACTTTCTCAA CAGTGGCCAG 
AACACAATGC TCATCAACTT GGGCCATCAG AAGCTAATGG ACTGTATCAT GACCCTACCT 
CGGTTCTACC AGGCTTCTAT TGTAGCTGAG GCCTATGACT TTGTTCCTGA TTGGGCTGAA 
GTACTCTACC AACAAGTGAT TCTTAAAGGA GACTTTAGTT ACTTGGAAGA ATTTAAGCAG 
CAGAAGTTAC TAAAACCCAA TATATTTGAA GACATTTCCA AAAAATATAA ACAACACCAG 
CCTACTGACA GGGTCACAGA AAACCTGAAG AAGTTACTTT CATATTGTGA AGACATCTAC 
CTGTATTACA AGTTGGCATA TGAACACAAG TTTTTTGAAA TTGTAAATAT GCTTCTGAAG 
GATCCTCAAA CAGGATGCTG TCTTAAAGAT ATGCTCGCGG GTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017447229.1
CDS	50..7354
Translation	MAAKPGPGSAASPGCAGAMERVLPMLLVRIPAEETAQLGPRAQLHRELEALGSLTAAGSLQVLSLAPGSRGGSSCRLQGPFRQFLWEESQNSTSADKPRVLVAREDCGLLIYEFDLRDGRCDAALLHSCCGRTLQKLTEDQGVSISFKSLRILSFDNSTSLLLINECLVVRVVFPGRESGIGVLGCLSLPLPAQAVDTIIDVQLCKRFLFLLSTLGWIHIFDTVKGMHVARVDLALPREDSEQQEPASISSFMSLKVSRDLDVLLLVSSSSTAVALNLHLYFRLHPGHLLCEGTLQDLPIEGPTGIDEDDLVNSAHNMKLTKVSFQVDRSWRAQLASLNESVRSSEPELSCYPPWFQGALCLESLESGNHSPIVPNHVFIPGDVPSGQYAFPQKEHSKTSEPRPWRTMHLSEQEQPTELTCLSVTGFTALFIQAVGTTSCTVVLWDLETQSMQCFSLSQKCTPVDISGDQQLCLALTENGLSLILFGLTQEEFLNRLMIHGSASTVDSLCHLNGWGRCSIPIHALEAGIENRQLDTVDFFLKSKENLLTPSSKSPAPDQQQHFSSQLYLRQVEEMTPALDLLCSAIRESDSETQSKHFAEQLLHLTLSFLNKQMRELFDHTEELDEHLQKGAAILTSYINELRTFMIKFPWKPGDAIDESDENEEVLTVKEGQVWEELRSEEVIADAILNNRIPEAQTFFRITGHSAQRLEELVRIGLDLAFDSLKKNSVEEASRLLRNMGFSVEDELLKISFYTTDKNIRDFLVEILKEKECFSEKERRTVDFVHQAERLYSGHFQENAPMQAFPRYWIKEQDCFKHKSVLDTFLKCDTKDEFTKQDHRIALNWAHWWDRQTQECILLPRISSEEYKAHSPEALWRHLTARHDWSSISLWMEEFQAQETVSPQQSKWPPLSAAVIEQNTCCSRHMRNAILDQLARCGIFLASELEDFELLLLRLSRIGGVMQDALPVQSYRSSAGCNFDSQVILYCLEHGLQHLLYVYLDYYKLSPGNCPFLEKKELHEAHPWLEFLVQCRQVSSNLTDPKLIFQASLANAQISIPTNQASVSSMLLEGHTLLALATTMYAPGGVSQVIQHEDSENCLKKVDPQLLKMALTPYPKLKAALFPQCTAPSILPSDITLYHLIQSLPPFDPSRLFGWQSANTLAIGDAAGQLPHFSSPDLVSKYAVVEQLSYTYYLHHGRPSFAFGTFLVQELIKSKTPKQLIQQVGKEAYTLGLSSFHNPSVGAACVCFLELLGLSSLKLRVDLKMANVVLGSRRRNEDAHPSFIRESLAEKLSKLADGDRATTEELLVLLEEGVWDSIEQQGFNRLSSESSSQWSLVLQFCMLHDMKLSVSYLRECAKANDWLQFLVHSQLHNYHPEEVETLLQYFSPVLQSHLKLASEKLSSGSISKDDSCLQELQKNKETSDFFEILHRCSDESASWCWLLAEAVRHRVPILSVLASCIQGASAVPCLCVWIVTSVEDKVAAEAIEHIQISVEDHHWSLEDLSIIWRTVLTRRKIHTLIRGFRLFIKDSPLLLIMEMYELCVFFKNYGKAKVKLVEFQKSLEMLDTVATRVLPVIPATWMREQVCFLLKLMPQQCETQYELQKVLQLFVGMEQLFSDGPDVQKLYRLSQVLKDTPITISPAVISSYSTESLQRECRSILEKLKADGQFAVARRVAELASLPVDGLVIEQLTQEMQMLTHTQQWSLKQARLDFWRKCHENFKKNSVSNRAASSFFSSQAPEASEHPAEQGSLEERHLLLTLAGHWLAQEEPVPVEELEELEKQIWLCRIAQHTCGGAEEETKPSLSQHKLAPAELSSDSSASEFSFSKLAALNTSKYLDLNGLPSKEMCENTLEHREQESLNTLIGQLLDGGCVHEASRVCRYFRVYSQDLVLLLHCRALASAEASMEDLHSEIRALLSNATLPEDLESPGVPLRKVHSNMCLLSASSLDSLSFAMVPPTDEVARSLQTLISRCLHGKDYCRQVLCLYELAKELGCSYGDVATRDSEAMLRAILASQRPDRCRQAQVFINTQGLEPDTVAELVAEEVTRELLTPSEGAGEKQPFNLAEESQTFLQLTALCQDRTLVGMKLLDKIPSVPHGELSCTTELLILAHHCFTCACYMEGIIRVLQAARMLTDNHLAPNEEYGLVVRLLTGIGRYNEMTYIFDLLHQKHYFEVLMRKKLDPTGTLKTALLDYIKRCHPGDSEKHNMIALCFSMCREIGENHEAAACIQLKLIESQPWEESLKDGAQLKQLLLKALTLMLDAAESYAKDSCVRQALHCNRLTKLITLQIHFLNSGQNTMLINLGHQKLMDCIMTLPRFYQASIVAEAYDFVPDWAEVLYQQVILKGDFSYLEEFKQQKLLKPNIFEDISKKYKQHQPTDRVTENLKKLLSYCEDIYLYYKLAYEHKFFEIVNMLLKDPQTGCCLKDMLAG

Target ORF information:

RefSeq Version	XM_017591740.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus spastic paraplegia 11 (autosomal recessive) (Spg11), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017591740.1

ORF Insert Sequence:

ATGGCTGCCA AGCCAGGGCC CGGAAGTGCT GCCTCCCCCG GCTGTGCCGG CGCCATGGAG 
CGAGTTCTAC CCATGCTGCT GGTGCGGATC CCTGCCGAGG AGACGGCGCA GCTGGGACCG 
CGGGCGCAGC TGCACCGAGA GCTGGAGGCC CTGGGGAGCC TGACGGCTGC GGGCAGTCTC 
CAAGTGCTTT CCTTGGCGCC GGGCAGTAGG GGTGGAAGCA GCTGCCGCCT CCAAGGCCCC 
TTTAGGCAGT TCCTGTGGGA GGAGTCTCAG AACAGCACAT CAGCAGACAA GCCCAGAGTG 
CTTGTTGCTC GAGAAGATTG TGGGCTGCTC ATATATGAAT TTGACTTGAG AGATGGAAGA 
TGTGACGCAG CCTTACTACA CAGCTGTTGT GGGAGGACCC TGCAGAAGCT CACTGAAGAT 
CAAGGTGTCA GCATTTCCTT CAAGTCTTTG AGGATTCTGT CATTTGACAA CAGCACGTCC 
TTACTGCTCA TCAATGAGTG TCTCGTCGTA CGTGTCGTGT TTCCGGGGAG AGAATCTGGG 
ATTGGAGTGC TGGGCTGCCT CTCACTGCCC TTGCCTGCAC AGGCAGTGGA CACGATAATT 
GACGTGCAGC TCTGCAAGAG GTTTCTTTTC CTTCTGAGTA CATTAGGCTG GATCCATATT 
TTTGACACTG TAAAGGGCAT GCATGTAGCC CGCGTGGACT TGGCTCTTCC CCGAGAAGAT 
TCTGAGCAGC AGGAGCCTGC CAGCATTTCT TCCTTTATGT CACTGAAAGT GTCTCGGGAC 
TTAGATGTTT TGCTACTCGT CAGCTCGTCC AGTACTGCAG TTGCTCTTAA CCTTCACTTG 
TATTTCAGGC TACACCCAGG GCACCTGCTG TGTGAAGGAA CTCTGCAAGA CCTCCCTATT 
GAAGGGCCTA CAGGAATAGA TGAAGATGAC CTTGTTAATT CTGCCCACAA CATGAAACTG 
ACCAAGGTGT CCTTCCAAGT TGATAGGTCC TGGAGAGCCC AGCTGGCATC ACTGAATGAA 
TCTGTGAGGA GCTCAGAACC AGAGCTTTCC TGCTATCCTC CGTGGTTCCA GGGTGCTTTG 
TGTTTGGAGT CCCTGGAATC TGGTAACCAC TCCCCCATTG TGCCAAACCA CGTTTTCATT 
CCAGGGGATG TCCCGAGTGG CCAGTATGCT TTTCCTCAGA AGGAGCACAG CAAGACCAGT 
GAGCCAAGGC CGTGGAGGAC AATGCACCTG AGTGAACAAG AGCAGCCCAC AGAGCTCACG 
TGTCTGTCTG TGACGGGATT TACGGCCCTG TTTATTCAGG CAGTAGGAAC GACCAGCTGC 
ACCGTCGTCC TCTGGGACTT GGAGACCCAG AGCATGCAGT GTTTTTCTCT CAGCCAGAAG 
TGTACTCCTG TAGACATCAG CGGAGACCAG CAGCTGTGCC TTGCTTTGAC AGAGAACGGC 
CTGTCTCTGA TTTTGTTTGG TTTAACTCAA GAGGAGTTTC TGAACAGACT GATGATCCAT 
GGCAGTGCCA GCACTGTGGA CTCCCTTTGT CATCTCAACG GTTGGGGACG GTGCTCAATC 
CCCATCCACG CTCTAGAGGC TGGCATAGAA AATCGTCAGC TGGACACGGT AGATTTCTTC 
CTGAAGAGCA AGGAAAATCT TCTTACTCCA TCTTCAAAAT CTCCTGCCCC TGATCAGCAG 
CAGCACTTCT CCTCCCAGCT CTACCTAAGG CAGGTGGAAG AGATGACCCC AGCCCTGGAC 
TTACTCTGCT CAGCAATTAG AGAAAGCGAC TCCGAAACCC AAAGCAAGCA CTTTGCAGAG 
CAGCTGCTTC ACCTCACACT GTCTTTCCTT AACAAGCAAA TGAGGGAGCT TTTTGATCAC 
ACTGAAGAGC TAGATGAGCA TTTACAGAAA GGAGCAGCCA TTTTGACCAG CTACATCAAT 
GAACTTCGGA CTTTTATGAT AAAGTTTCCT TGGAAGCCAG GAGATGCCAT AGATGAATCT 
GATGAAAATG AAGAGGTCCT CACAGTGAAA GAAGGCCAGG TTTGGGAGGA ACTCCGCTCT 
GAGGAAGTTA TTGCTGATGC CATTTTAAAT AACAGAATAC CAGAGGCACA AACTTTCTTC 
AGGATTACTG GTCATTCCGC TCAACGACTT GAGGAACTGG TTAGGATAGG CCTAGATTTG 
GCATTTGACA GTTTAAAAAA GAACAGCGTG GAGGAGGCCT CCAGGCTCTT GAGGAACATG 
GGCTTCAGTG TGGAAGACGA GCTGCTTAAG ATAAGCTTTT ATACGACTGA TAAAAACATA 
CGGGACTTTT TGGTTGAAAT TTTAAAAGAA AAGGAATGTT TTTCTGAAAA AGAAAGAAGA 
ACTGTGGACT TTGTCCATCA AGCTGAAAGG CTTTACTCAG GACACTTCCA GGAAAATGCG 
CCGATGCAGG CTTTTCCCAG GTATTGGATA AAGGAACAGG ACTGTTTCAA ACACAAATCT 
GTCTTGGACA CGTTCCTCAA ATGTGATACA AAAGATGAGT TTACCAAACA GGACCATAGA 
ATTGCACTGA ATTGGGCTCA CTGGTGGGAT CGGCAAACAC AGGAGTGTAT CCTTCTCCCC 
AGAATAAGTT CAGAAGAATA TAAAGCACAT TCCCCAGAAG CCCTGTGGAG ACACCTCACA 
GCTCGCCATG ACTGGTCAAG CATCAGCTTG TGGATGGAAG AATTTCAGGC CCAGGAAACT 
GTGTCACCTC AGCAGAGCAA GTGGCCTCCT CTGAGTGCTG CCGTCATCGA GCAGAACACT 
TGCTGCAGCA GGCACATGAG GAACGCGATC TTAGACCAGC TGGCCAGATG TGGCATTTTT 
CTTGCATCTG AGCTGGAAGA CTTTGAACTT CTCCTCCTGA GACTGAGCCG GATTGGGGGT 
GTAATGCAGG ATGCCCTCCC CGTGCAGAGC TACAGATCCT CGGCAGGGTG CAACTTTGAT 
TCCCAAGTCA TCCTCTACTG TTTAGAGCAC GGCCTGCAGC ATCTTCTCTA CGTCTACCTT 
GACTATTACA AACTTAGCCC TGGAAATTGT CCCTTTTTGG AAAAGAAAGA ACTACATGAA 
GCTCACCCTT GGCTTGAATT TTTGGTTCAG TGTCGGCAAG TTTCCAGTAA CTTGACAGAT 
CCCAAACTGA TCTTTCAGGC CAGTCTTGCA AATGCTCAGA TTTCGATTCC CACCAATCAA 
GCCAGTGTGA GCAGCATGCT GTTGGAAGGA CACACCCTCT TGGCTCTTGC TACCACCATG 
TATGCTCCTG GGGGCGTCAG CCAGGTCATT CAGCATGAAG ACAGCGAGAA CTGTTTGAAG 
AAAGTGGACC CCCAGCTACT GAAGATGGCC TTAACGCCTT ACCCAAAGCT CAAAGCCGCG 
CTCTTCCCTC AGTGCACTGC TCCTAGTATT CTGCCCTCTG ACATTACACT CTACCACCTC 
ATCCAGTCCT TACCACCCTT TGATCCCAGC AGACTGTTTG GCTGGCAGTC TGCCAACACT 
CTAGCTATCG GAGATGCGGC CGGTCAGCTC CCACACTTCT CCAGTCCTGA CCTGGTGAGT 
AAATACGCCG TGGTGGAGCA GCTGAGCTAC ACGTATTACT TACATCACGG ACGCCCATCC 
TTCGCCTTCG GCACCTTTCT GGTCCAGGAG TTAATCAAGA GTAAGACTCC CAAGCAGCTG 
ATCCAGCAAG TAGGCAAGGA GGCCTATACC TTAGGGCTCT CCTCCTTCCA CAACCCTTCG 
GTAGGGGCCG CCTGTGTCTG CTTCTTAGAA TTGCTTGGCC TCAGCAGCCT CAAGCTCAGA 
GTTGACCTGA AAATGGCAAA CGTAGTTCTG GGCTCCAGGC GTAGAAATGA AGATGCACAT 
CCCAGCTTCA TCAGAGAGTC TCTGGCTGAG AAGCTGTCTA AGCTTGCTGA CGGTGACAGG 
GCAACCACAG AAGAGCTGCT CGTTCTCTTG GAGGAAGGCG TGTGGGACAG CATTGAGCAG 
CAGGGCTTTA ACAGGTTGTC CAGCGAATCC AGCAGCCAGT GGTCATTAGT GTTACAGTTC 
TGCATGCTCC ATGATATGAA ACTGAGCGTG TCCTACCTTA GAGAATGTGC CAAAGCAAAT 
GATTGGCTGC AGTTTCTGGT TCACAGCCAG CTCCATAACT ACCACCCAGA AGAGGTGGAA 
ACCCTGCTTC AGTATTTCAG TCCTGTCCTT CAGAGTCACT TGAAGCTGGC TTCTGAGAAA 
CTGTCATCAG GGTCCATCTC TAAGGACGAC AGCTGCCTCC AGGAACTCCA GAAAAACAAA 
GAAACAAGTG ATTTCTTTGA AATTCTCCAC CGGTGCTCGG ATGAGTCAGC TTCCTGGTGC 
TGGCTCCTGG CTGAAGCAGT GAGACATCGG GTTCCCATCC TCAGTGTCCT GGCCTCGTGT 
ATCCAGGGTG CCAGTGCTGT CCCCTGTCTC TGTGTTTGGA TTGTCACCTC TGTGGAGGAC 
AAGGTTGCAG CTGAAGCAAT AGAACACATT CAGATCTCTG TAGAGGACCA TCATTGGAGC 
CTCGAGGACC TGTCCATTAT CTGGAGAACT GTATTGACAA GACGGAAGAT CCACACTCTC 
ATCCGAGGCT TCCGGCTTTT CATTAAGGAT TCCCCACTTC TCTTGATTAT GGAAATGTAT 
GAACTGTGTG TGTTCTTCAA GAACTACGGA AAAGCCAAAG TTAAGCTTGT GGAGTTCCAG 
AAAAGCCTTG AAATGCTTGA CACAGTGGCT ACTCGGGTCC TTCCTGTCAT ACCTGCCACG 
TGGATGAGGG AGCAGGTGTG CTTCCTTTTG AAACTTATGC CACAGCAGTG CGAGACACAG 
TATGAGCTGC AGAAAGTCCT CCAACTGTTT GTCGGGATGG AGCAGCTCTT CTCCGATGGG 
CCAGACGTGC AGAAGCTGTA TCGGCTCAGC CAAGTACTGA AGGACACACC TATCACCATC 
AGCCCTGCAG TCATTTCCAG CTACAGCACT GAGAGTTTGC AGCGTGAGTG TAGATCTATT 
TTGGAGAAGC TAAAGGCAGA TGGACAGTTT GCTGTGGCCA GGAGAGTGGC AGAATTAGCA 
TCCCTGCCTG TGGACGGCTT GGTGATTGAA CAGTTAACGC AGGAAATGCA AATGCTAACA 
CACACCCAGC AGTGGTCCCT GAAACAAGCA AGACTTGACT TCTGGAGAAA ATGCCATGAA 
AATTTTAAGA AAAACTCTGT TTCAAACAGA GCAGCCTCTT CCTTCTTCTC CAGCCAAGCC 
CCGGAGGCGA GTGAGCACCC TGCTGAGCAG GGCAGCCTTG AGGAGCGCCA TCTGCTGCTC 
ACTCTGGCGG GCCACTGGCT TGCCCAAGAA GAGCCAGTTC CCGTGGAGGA ACTGGAGGAG 
CTGGAGAAAC AGATTTGGCT CTGCCGCATC GCCCAGCACA CATGTGGAGG GGCTGAAGAA 
GAAACCAAGC CCAGCCTCTC GCAACACAAG TTGGCCCCTG CTGAACTTTC TTCTGATAGC 
TCAGCCAGTG AGTTCTCCTT CTCCAAGCTT GCTGCTTTGA ACACATCCAA ATACTTAGAC 
CTTAATGGTC TTCCATCCAA GGAGATGTGT GAGAACACAC TGGAACACAG AGAGCAGGAG 
TCACTGAACA CTTTGATTGG GCAGCTGCTG GATGGTGGCT GTGTGCATGA AGCGAGCAGA 
GTGTGCCGGT ATTTCCGTGT CTATAGTCAG GATCTAGTGC TGCTGCTGCA CTGCAGAGCC 
TTGGCCTCCG CAGAGGCCAG CATGGAAGAT CTGCACTCAG AGATCCGTGC CCTCCTCTCA 
AATGCCACAC TACCTGAGGA CCTGGAGAGC CCTGGCGTCC CTCTTCGGAA AGTCCATAGC 
AACATGTGTT TGCTTTCAGC TTCGAGTTTG GACAGTCTGT CATTTGCGAT GGTACCACCT 
ACTGATGAAG TGGCCAGGAG TCTCCAAACT CTCATAAGCA GGTGCCTCCA TGGGAAGGAT 
TACTGTCGGC AGGTCCTCTG TCTGTATGAA CTTGCCAAGG AGTTGGGCTG TTCCTATGGA 
GATGTTGCTA CCAGGGACAG TGAAGCCATG CTCCGAGCAA TCTTGGCTTC TCAGCGGCCT 
GACCGATGCA GACAAGCCCA GGTCTTCATC AACACCCAGG GCCTTGAACC AGACACTGTG 
GCTGAACTTG TGGCTGAAGA GGTGACACGA GAGCTACTGA CCCCATCAGA GGGAGCAGGA 
GAAAAGCAGC CTTTCAACCT AGCAGAGGAA AGCCAGACAT TTCTTCAGCT AACTGCTTTG 
TGTCAAGACC GTACATTGGT AGGCATGAAG TTGTTGGACA AGATTCCATC TGTCCCCCAT 
GGGGAGCTGT CATGCACCAC AGAGCTCCTG ATCCTGGCCC ATCACTGCTT TACCTGTGCG 
TGTTACATGG AGGGCATCAT CAGAGTCCTA CAGGCTGCCC GGATGCTTAC AGATAACCAC 
TTGGCTCCCA ATGAGGAGTA TGGATTGGTG GTACGCCTCC TCACTGGCAT TGGACGGTAC 
AATGAGATGA CGTACATATT CGACTTGCTG CATCAAAAGC ATTATTTTGA AGTTCTGATG 
AGGAAGAAGC TAGATCCAAC CGGCACCCTG AAAACAGCCC TACTAGACTA CATCAAACGC 
TGCCACCCTG GAGACAGTGA AAAGCACAAT ATGATCGCCC TGTGCTTCAG CATGTGTCGG 
GAGATAGGAG AGAACCACGA GGCCGCAGCC TGCATCCAAC TGAAGCTGAT CGAATCTCAG 
CCCTGGGAGG AAAGTCTCAA GGATGGAGCC CAACTAAAAC AGCTCCTGCT TAAAGCCCTG 
ACTTTGATGC TGGACGCAGC AGAGAGTTAC GCAAAGGACT CCTGTGTGCG GCAGGCCCTG 
CATTGTAACC GGCTCACCAA GTTGATAACA CTACAGATTC ACTTTCTCAA CAGTGGCCAG 
AACACAATGC TCATCAACTT GGGCCATCAG AAGCTAATGG ACTGTATCAT GACCCTACCT 
CGGTTCTACC AGGCTTCTAT TGTAGCTGAG GCCTATGACT TTGTTCCTGA TTGGGCTGAA 
GTACTCTACC AACAAGTGAT TCTTAAAGGA GACTTTAGTT ACTTGGAAGA ATTTAAGCAG 
CAGAAGTTAC TAAAACCCAA TATATTTGAA GACATTTCCA AAAAATATAA ACAACACCAG 
CCTACTGACA GGGTCACAGA AAACCTGAAG AAGTTACTTT CATATTGTGA AGACATCTAC 
CTGTATTACA AGTTGGCATA TGAACACAAG TTTTTTGAAA TTGTAAATAT GCTTCTGAAG 
GATCCTCAAA CAGGATGCTG TCTTAAAGAT ATGCTCGCGG GTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Spg11 ( XM_006234855.3 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006234917.2 Rattus norvegicus spastic paraplegia 11 (autosomal recessive) (Spg11), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ORa60400
Clone ID Related Accession (Same CDS sequence)	XM_006234855.3
Accession Version	XM_006234855.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7287bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1469376000000
Organism	Rattus norvegicus(Norway rat)
Product	spatacsin isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005102.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006234855.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTGCCA AGCCAGGGCC CGGAAGTGCT GCCTCCCCCG GCTGTGCCGG CGCCATGGAG 
CGAGTTCTAC CCATGCTGCT GGTGCGGATC CCTGCCGAGG AGACGGCGCA GCTGGGACCG 
CGGGCGCAGC TGCACCGAGA GCTGGAGGCC CTGGGGAGCC TGACGGCTGC GGGCAGTCTC 
CAAGTGCTTT CCTTGGCGCC GGGCAGTAGG GGTGGAAGCA GCTGCCGCCT CCAAGGCCCC 
TTTAGGCAGT TCCTGTGGGA GGAGTCTCAG AACAGCACAT CAGCAGACAA GCCCAGAGTG 
CTTGTTGCTC GAGAAGATTG TGGGCTGCTC ATATATGAAT TTGACTTGAG AGATGGAAGA 
TGTGACGCAG CCTTACTACA CAGCTGTTGT GGGAGGACCC TGCAGAAGCT CACTGAAGAT 
CAAGGTGTCA GCATTTCCTT CAAGTCTTTG AGGATTCTGT CATTTGACAA CAGCACGTCC 
TTACTGCTCA TCAATGAGTG TCTCGTCGTA CGTGTCGTGT TTCCGGGGAG AGAATCTGGG 
ATTGGAGTGC TGGGCTGCCT CTCACTGCCC TTGCCTGCAC AGGCAGTGGA CACGATAATT 
GACGTGCAGC TCTGCAAGAG GTTTCTTTTC CTTCTGAGTA CATTAGGCTG GATCCATATT 
TTTGACACTG TAAAGGGCAT GCATGTAGCC CGCGTGGACT TGGCTCTTCC CCGAGAAGAT 
TCTGAGCAGC AGGAGCCTGC CAGCATTTCT TCCTTTATGT CACTGAAAGT GTCTCGGGAC 
TTAGATGTTT TGCTACTCGT CAGCTCGTCC AGTACTGCAG TTGCTCTTAA CCTTCACTTG 
TATTTCAGGC TACACCCAGG GCACCTGCTG TGTGAAGGAA CTCTGCAAGA CCTCCCTATT 
GAAGGGCCTA CAGGAATAGA TGAAGATGAC CTTGTTAATT CTGCCCACAA CATGAAACTG 
ACCAAGGTGT CCTTCCAAGT TGATAGGTCC TGGAGAGCCC AGCTGGCATC ACTGAATGAA 
TCTGTGAGGA GCTCAGAACC AGAGCTTTCC TGCTATCCTC CGTGGTTCCA GGGTGCTTTG 
TGTTTGGAGT CCCTGGAATC TGGTAACCAC TCCCCCATTG TGCCAAACCA CGTTTTCATT 
CCAGGGGATG TCCCGAGTGG CCAGTATGCT TTTCCTCAGA AGGAGCACAG CAAGACCAGT 
GAGCCAAGGC CGTGGAGGAC AATGCACCTG AGTGAACAAG AGCAGCCCAC AGAGCTCACG 
TGTCTGTCTG TGACGGGATT TACGGCCCTG TTTATTCAGG CAGTAGGAAC GACCAGCTGC 
ACCGTCGTCC TCTGGGACTT GGAGACCCAG AGCATGCAGT GTTTTTCTCT CAGCCAGAAG 
TGTACTCCTG TAGACATCAG CGGAGACCAG CAGCTGTGCC TTGCTTTGAC AGAGAACGGC 
CTGTCTCTGA TTTTGTTTGG TTTAACTCAA GAGGAGTTTC TGAACAGACT GATGATCCAT 
GGCAGTGCCA GCACTGTGGA CTCCCTTTGT CATCTCAACG GTTGGGGACG GTGCTCAATC 
CCCATCCACG CTCTAGAGGC TGGCATAGAA AATCGTCAGC TGGACACGGT AGATTTCTTC 
CTGAAGAGCA AGGAAAATCT TCTTACTCCA TCTTCAAAAT CTCCTGCCCC TGATCAGCAG 
CAGCACTTCT CCTCCCAGCT CTACCTAAGG CAGGTGGAAG AGATGACCCC AGCCCTGGAC 
TTACTCTGCT CAGCAATTAG AGAAAGCGAC TCCGAAACCC AAAGCAAGCA CTTTGCAGAG 
CAGCTGCTTC ACCTCACACT GTCTTTCCTT AACAAGCAAA TGAGGGAGCT TTTTGATCAC 
ACTGAAGAGC TAGATGAGCA TTTACAGAAA GGAGCAGCCA TTTTGACCAG CTACATCAAT 
GAACTTCGGA CTTTTATGAT AAAGTTTCCT TGGAAGCCAG GAGATGCCAT AGATGAATCT 
GATGAAAATG AAGAGGTCCT CACAGTGAAA GAAGGCCAGG TTTGGGAGGA ACTCCGCTCT 
GAGGAAGTTA TTGCTGATGC CATTTTAAAT AACAGAATAC CAGAGGCACA AACTTTCTTC 
AGGATTACTG GTCATTCCGC TCAACGACTT GAGGAACTGG TTAGGATAGG CCTAGATTTG 
GCATTTGACA GTTTAAAAAA GAACAGCGTG GAGGAGGCCT CCAGGCTCTT GAGGAACATG 
GGCTTCAGTG TGGAAGACGA GCTGCTTAAG ATAAGCTTTT ATACGACTGA TAAAAACATA 
CGGGACTTTT TGGTTGAAAT TTTAAAAGAA AAGGAATGTT TTTCTGAAAA AGAAAGAAGA 
ACTGTGGACT TTGTCCATCA AGCTGAAAGG CTTTACTCAG GACACTTCCA GGAAAATGCG 
CCGATGCAGG CTTTTCCCAG GTATTGGATA AAGGAACAGG ACTGTTTCAA ACACAAATCT 
GTCTTGGACA CGTTCCTCAA ATGTGATACA AAAGATGAGT TTACCAAACA GGACCATAGA 
ATTGCACTGA ATTGGGCTCA CTGGTGGGAT CGGCAAACAC AGGAGTGTAT CCTTCTCCCC 
AGAATAAGTT CAGAAGAATA TAAAGCACAT TCCCCAGAAG CCCTGTGGAG ACACCTCACA 
GCTCGCCATG ACTGGTCAAG CATCAGCTTG TGGATGGAAG AATTTCAGGC CCAGGAAACT 
GTGTCACCTC AGCAGAGCAA GTGGCCTCCT CTGAGTGCTG CCGTCATCGA GCAGAACACT 
TGCTGCAGCA GGCACATGAG GAACGCGATC TTAGACCAGC TGGCCAGATG TGGCATTTTT 
CTTGCATCTG AGCTGGAAGA CTTTGAACTT CTCCTCCTGA GACTGAGCCG GATTGGGGGT 
GTAATGCAGG ATGCCCTCCC CGTGCAGAGC TACAGATCCT CGGCAGGGTG CAACTTTGAT 
TCCCAAGTCA TCCTCTACTG TTTAGAGCAC GGCCTGCAGC ATCTTCTCTA CGTCTACCTT 
GACTATTACA AACTTAGCCC TGGAAATTGT CCCTTTTTGG AAAAGAAAGA ACTACATGAA 
GCTCACCCTT GGCTTGAATT TTTGGTTCAG TGTCGGCAAG TTTCCAGTAA CTTGACAGAT 
CCCAAACTGA TCTTTCAGGC CAGTCTTGCA AATGCTCAGA TTTCGATTCC CACCAATCAA 
GCCAGTGTGA GCAGCATGCT GTTGGAAGGA CACACCCTCT TGGCTCTTGC TACCACCATG 
TATGCTCCTG GGGGCGTCAG CCAGGTCATT CAGCATGAAG ACAGCGAGAA CTGTTTGAAG 
AAAGTGGACC CCCAGCTACT GAAGATGGCC TTAACGCCTT ACCCAAAGCT CAAAGCCGCG 
CTCTTCCCTC AGTGCACTGC TCCTAGTATT CTGCCCTCTG ACATTACACT CTACCACCTC 
ATCCAGTCCT TACCACCCTT TGATCCCAGC AGACTGTTTG GCTGGCAGTC TGCCAACACT 
CTAGCTATCG GAGATGCGGC CGGTCAGCTC CCACACTTCT CCAGTCCTGA CCTGGTGAGT 
AAATACGCCG TGGTGGAGCA GCTGAGCTAC ACGTATTACT TACATCACGG ACGCCCATCC 
TTCGCCTTCG GCACCTTTCT GGTCCAGGAG TTAATCAAGA GTAAGACTCC CAAGCAGCTG 
ATCCAGCAAG TAGGCAAGGA GGCCTATACC TTAGGGCTCT CCTCCTTCCA CAACCCTTCG 
GTAGGGGCCG CCTGTGTCTG CTTCTTAGAA TTGCTTGGCC TCAGCAGCCT CAAGCTCAGA 
GTTGACCTGA AAATGGCAAA CGTAGTTCTG GGCTCCAGGC GTAGAAATGA AGATGCACAT 
CCCAGCTTCA TCAGAGAGTC TCTGGCTGAG AAGCTGTCTA AGCTTGCTGA CGGTGACAGG 
GCAACCACAG AAGAGCTGCT CGTTCTCTTG GAGGAAGGCG TGTGGGACAG CATTGAGCAG 
CAGGGCTTTA ACAGGTTGTC CAGCGAATCC AGCAGCCAGT GGTCATTAGT GTTACAGTTC 
TGCATGCTCC ATGATATGAA ACTGAGCGTG TCCTACCTTA GAGAATGTGC CAAAGCAAAT 
GATTGGCTGC AGTTTCTGGT TCACAGCCAG CTCCATAACT ACCACCCAGA AGAGGTGGAA 
ACCCTGCTTC AGTATTTCAG TCCTGTCCTT CAGAGTCACT TGAAGCTGGC TTCTGAGAAA 
CTGTCATCAG GGTCCATCTC TAAGGACGAC AGCTGCCTCC AGGAACTCCA GAAAAACAAA 
GAAACAAGTG ATTTCTTTGA AATTCTCCAC CGGTGCTCGG ATGAGTCAGC TTCCTGGTGC 
TGGCTCCTGG CTGAAGCAGT GAGACATCGG GTTCCCATCC TCAGTGTCCT GGCCTCGTGT 
ATCCAGGGTG CCAGTGCTGT CCCCTGTCTC TGTGTTTGGA TTGTCACCTC TGTGGAGGAC 
AAGGTTGCAG CTGAAGCAAT AGAACACATT CAGATCTCTG TAGAGGACCA TCATTGGAGC 
CTCGAGGACC TGTCCATTAT CTGGAGAACT GTATTGACAA GACGGAAGAT CCACACTCTC 
ATCCGAGGCT TCCGGCTTTT CATTAAGGAT TCCCCACTTC TCTTGATTAT GGAAATGTAT 
GAACTGTGTG TGTTCTTCAA GAACTACGGA AAAGCCAAAG TTAAGCTTGT GGAGTTCCAG 
AAAAGCCTTG AAATGCTTGA CACAGTGGCT ACTCGGGTCC TTCCTGTCAT ACCTGCCACG 
TGGATGAGGG AGCAGGTGTG CTTCCTTTTG AAACTTATGC CACAGCAGTG CGAGACACAG 
TATGAGCTGC AGAAAGTCCT CCAACTGTTT GTCGGGATGG AGCAGCTCTT CTCCGATGGG 
CCAGACGTGC AGAAGCTGTA TCGGCTCAGC CAAGTACTGA AGGACACACC TATCACCATC 
AGCCCTGCAG TCATTTCCAG CTACAGCACT GAGAGTTTGC AGCGTGAGTG TAGATCTATT 
TTGGAGAAGC TAAAGGCAGA TGGACAGTTT GCTGTGGCCA GGAGAGTGGC AGAATTAGCA 
TCCCTGCCTG TGGACGGCTT GGTGATTGAA CAGTTAACGC AGGAAATGCA AATGCTAACA 
CACACCCAGC AGTGGTCCCT GAAACAAGCA AGACTTGACT TCTGGAGAAA ATGCCATGAA 
AATTTTAAGA AAAACTCTGT TTCAAACAGA GCAGCCTCTT CCTTCTTCTC CAGCCAAGCC 
CCGGAGGCGA GTGAGCACCC TGCTGAGCAG GGCAGCCTTG AGGAGCGCCA TCTGCTGCTC 
ACTCTGGCGG GCCACTGGCT TGCCCAAGAA GAGCCAGTTC CCGTGGAGGA ACTGGAGGAG 
CTGGAGAAAC AGATTTGGCT CTGCCGCATC GCCCAGCACA CATGTGGAGG GGCTGAAGAA 
GAAACCAAGC CCAGCCTCTC GCAACACAAG TTGGCCCCTG CTGAACTTTC TTCTGATAGC 
TCAGCCAGTG AGTTCTCCTT CTCCAAGCTT GCTGCTTTGA ACACATCCAA ATACTTAGAC 
CTTAATGGTC TTCCATCCAA GGAGATGTGT GAGAACACAC TGGAACACAG AGAGCAGGAG 
TCACTGAACA CTTTGATTGG GCAGCTGCTG GATGGTGGCT GTGTGCATGA AGCGAGCAGA 
GTGTGCCGGT ATTTCCGTGT CTATAGTCAG GATCTAGTGC TGCTGCTGCA CTGCAGAGCC 
TTGGCCTCCG CAGAGGCCAG CATGGAAGAT CTGCACTCAG AGATCCGTGC CCTCCTCTCA 
AATGCCACAC TACCTGAGGA CCTGGAGAGC CCTGGCGTCC CTCTTCGGAA AGTCCATAGC 
ACTTCGAGTT TGGACAGTCT GTCATTTGCG ATGGTACCAC CTACTGATGA AGTGGCCAGG 
AGTCTCCAAA CTCTCATAAG CAGGTGCCTC CATGGGAAGG ATTACTGTCG GCAGGTCCTC 
TGTCTGTATG AACTTGCCAA GGAGTTGGGC TGTTCCTATG GAGATGTTGC TACCAGGGAC 
AGTGAAGCCA TGCTCCGAGC AATCTTGGCT TCTCAGCGGC CTGACCGATG CAGACAAGCC 
CAGGTCTTCA TCAACACCCA GGGCCTTGAA CCAGACACTG TGGCTGAACT TGTGGCTGAA 
GAGGTGACAC GAGAGCTACT GACCCCATCA GAGGGAGCAG GAGAAAAGCA GCCTTTCAAC 
CTAGCAGAGG AAAGCCAGAC ATTTCTTCAG CTAACTGCTT TGTGTCAAGA CCGTACATTG 
GTAGGCATGA AGTTGTTGGA CAAGATTCCA TCTGTCCCCC ATGGGGAGCT GTCATGCACC 
ACAGAGCTCC TGATCCTGGC CCATCACTGC TTTACCTGTG CGTGTTACAT GGAGGGCATC 
ATCAGAGTCC TACAGGCTGC CCGGATGCTT ACAGATAACC ACTTGGCTCC CAATGAGGAG 
TATGGATTGG TGGTACGCCT CCTCACTGGC ATTGGACGGT ACAATGAGAT GACGTACATA 
TTCGACTTGC TGCATCAAAA GCATTATTTT GAAGTTCTGA TGAGGAAGAA GCTAGATCCA 
ACCGGCACCC TGAAAACAGC CCTACTAGAC TACATCAAAC GCTGCCACCC TGGAGACAGT 
GAAAAGCACA ATATGATCGC CCTGTGCTTC AGCATGTGTC GGGAGATAGG AGAGAACCAC 
GAGGCCGCAG CCTGCATCCA ACTGAAGCTG ATCGAATCTC AGCCCTGGGA GGAAAGTCTC 
AAGGATGGAG CCCAACTAAA ACAGCTCCTG CTTAAAGCCC TGACTTTGAT GCTGGACGCA 
GCAGAGAGTT ACGCAAAGGA CTCCTGTGTG CGGCAGGCCC TGCATTGTAA CCGGCTCACC 
AAGTTGATAA CACTACAGAT TCACTTTCTC AACAGTGGCC AGAACACAAT GCTCATCAAC 
TTGGGCCATC AGAAGCTAAT GGACTGTATC ATGACCCTAC CTCGGTTCTA CCAGGCTTCT 
ATTGTAGCTG AGGCCTATGA CTTTGTTCCT GATTGGGCTG AAGTACTCTA CCAACAAGTG 
ATTCTTAAAG GAGACTTTAG TTACTTGGAA GAATTTAAGC AGCAGAAGTT ACTAAAACCC 
AATATATTTG AAGACATTTC CAAAAAATAT AAACAACACC AGCCTACTGA CAGGGTCACA 
GAAAACCTGA AGAAGTTACT TTCATATTGT GAAGACATCT ACCTGTATTA CAAGTTGGCA 
TATGAACACA AGTTTTTTGA AATTGTAAAT ATGCTTCTGA AGGATCCTCA AACAGGATGC 
TGTCTTAAAG ATATGCTCGC GGGTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006234917.2
CDS	50..7336
Translation	MAAKPGPGSAASPGCAGAMERVLPMLLVRIPAEETAQLGPRAQLHRELEALGSLTAAGSLQVLSLAPGSRGGSSCRLQGPFRQFLWEESQNSTSADKPRVLVAREDCGLLIYEFDLRDGRCDAALLHSCCGRTLQKLTEDQGVSISFKSLRILSFDNSTSLLLINECLVVRVVFPGRESGIGVLGCLSLPLPAQAVDTIIDVQLCKRFLFLLSTLGWIHIFDTVKGMHVARVDLALPREDSEQQEPASISSFMSLKVSRDLDVLLLVSSSSTAVALNLHLYFRLHPGHLLCEGTLQDLPIEGPTGIDEDDLVNSAHNMKLTKVSFQVDRSWRAQLASLNESVRSSEPELSCYPPWFQGALCLESLESGNHSPIVPNHVFIPGDVPSGQYAFPQKEHSKTSEPRPWRTMHLSEQEQPTELTCLSVTGFTALFIQAVGTTSCTVVLWDLETQSMQCFSLSQKCTPVDISGDQQLCLALTENGLSLILFGLTQEEFLNRLMIHGSASTVDSLCHLNGWGRCSIPIHALEAGIENRQLDTVDFFLKSKENLLTPSSKSPAPDQQQHFSSQLYLRQVEEMTPALDLLCSAIRESDSETQSKHFAEQLLHLTLSFLNKQMRELFDHTEELDEHLQKGAAILTSYINELRTFMIKFPWKPGDAIDESDENEEVLTVKEGQVWEELRSEEVIADAILNNRIPEAQTFFRITGHSAQRLEELVRIGLDLAFDSLKKNSVEEASRLLRNMGFSVEDELLKISFYTTDKNIRDFLVEILKEKECFSEKERRTVDFVHQAERLYSGHFQENAPMQAFPRYWIKEQDCFKHKSVLDTFLKCDTKDEFTKQDHRIALNWAHWWDRQTQECILLPRISSEEYKAHSPEALWRHLTARHDWSSISLWMEEFQAQETVSPQQSKWPPLSAAVIEQNTCCSRHMRNAILDQLARCGIFLASELEDFELLLLRLSRIGGVMQDALPVQSYRSSAGCNFDSQVILYCLEHGLQHLLYVYLDYYKLSPGNCPFLEKKELHEAHPWLEFLVQCRQVSSNLTDPKLIFQASLANAQISIPTNQASVSSMLLEGHTLLALATTMYAPGGVSQVIQHEDSENCLKKVDPQLLKMALTPYPKLKAALFPQCTAPSILPSDITLYHLIQSLPPFDPSRLFGWQSANTLAIGDAAGQLPHFSSPDLVSKYAVVEQLSYTYYLHHGRPSFAFGTFLVQELIKSKTPKQLIQQVGKEAYTLGLSSFHNPSVGAACVCFLELLGLSSLKLRVDLKMANVVLGSRRRNEDAHPSFIRESLAEKLSKLADGDRATTEELLVLLEEGVWDSIEQQGFNRLSSESSSQWSLVLQFCMLHDMKLSVSYLRECAKANDWLQFLVHSQLHNYHPEEVETLLQYFSPVLQSHLKLASEKLSSGSISKDDSCLQELQKNKETSDFFEILHRCSDESASWCWLLAEAVRHRVPILSVLASCIQGASAVPCLCVWIVTSVEDKVAAEAIEHIQISVEDHHWSLEDLSIIWRTVLTRRKIHTLIRGFRLFIKDSPLLLIMEMYELCVFFKNYGKAKVKLVEFQKSLEMLDTVATRVLPVIPATWMREQVCFLLKLMPQQCETQYELQKVLQLFVGMEQLFSDGPDVQKLYRLSQVLKDTPITISPAVISSYSTESLQRECRSILEKLKADGQFAVARRVAELASLPVDGLVIEQLTQEMQMLTHTQQWSLKQARLDFWRKCHENFKKNSVSNRAASSFFSSQAPEASEHPAEQGSLEERHLLLTLAGHWLAQEEPVPVEELEELEKQIWLCRIAQHTCGGAEEETKPSLSQHKLAPAELSSDSSASEFSFSKLAALNTSKYLDLNGLPSKEMCENTLEHREQESLNTLIGQLLDGGCVHEASRVCRYFRVYSQDLVLLLHCRALASAEASMEDLHSEIRALLSNATLPEDLESPGVPLRKVHSTSSLDSLSFAMVPPTDEVARSLQTLISRCLHGKDYCRQVLCLYELAKELGCSYGDVATRDSEAMLRAILASQRPDRCRQAQVFINTQGLEPDTVAELVAEEVTRELLTPSEGAGEKQPFNLAEESQTFLQLTALCQDRTLVGMKLLDKIPSVPHGELSCTTELLILAHHCFTCACYMEGIIRVLQAARMLTDNHLAPNEEYGLVVRLLTGIGRYNEMTYIFDLLHQKHYFEVLMRKKLDPTGTLKTALLDYIKRCHPGDSEKHNMIALCFSMCREIGENHEAAACIQLKLIESQPWEESLKDGAQLKQLLLKALTLMLDAAESYAKDSCVRQALHCNRLTKLITLQIHFLNSGQNTMLINLGHQKLMDCIMTLPRFYQASIVAEAYDFVPDWAEVLYQQVILKGDFSYLEEFKQQKLLKPNIFEDISKKYKQHQPTDRVTENLKKLLSYCEDIYLYYKLAYEHKFFEIVNMLLKDPQTGCCLKDMLAG

Target ORF information:

RefSeq Version	XM_006234855.3
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus spastic paraplegia 11 (autosomal recessive) (Spg11), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006234855.3

ORF Insert Sequence:

ATGGCTGCCA AGCCAGGGCC CGGAAGTGCT GCCTCCCCCG GCTGTGCCGG CGCCATGGAG 
CGAGTTCTAC CCATGCTGCT GGTGCGGATC CCTGCCGAGG AGACGGCGCA GCTGGGACCG 
CGGGCGCAGC TGCACCGAGA GCTGGAGGCC CTGGGGAGCC TGACGGCTGC GGGCAGTCTC 
CAAGTGCTTT CCTTGGCGCC GGGCAGTAGG GGTGGAAGCA GCTGCCGCCT CCAAGGCCCC 
TTTAGGCAGT TCCTGTGGGA GGAGTCTCAG AACAGCACAT CAGCAGACAA GCCCAGAGTG 
CTTGTTGCTC GAGAAGATTG TGGGCTGCTC ATATATGAAT TTGACTTGAG AGATGGAAGA 
TGTGACGCAG CCTTACTACA CAGCTGTTGT GGGAGGACCC TGCAGAAGCT CACTGAAGAT 
CAAGGTGTCA GCATTTCCTT CAAGTCTTTG AGGATTCTGT CATTTGACAA CAGCACGTCC 
TTACTGCTCA TCAATGAGTG TCTCGTCGTA CGTGTCGTGT TTCCGGGGAG AGAATCTGGG 
ATTGGAGTGC TGGGCTGCCT CTCACTGCCC TTGCCTGCAC AGGCAGTGGA CACGATAATT 
GACGTGCAGC TCTGCAAGAG GTTTCTTTTC CTTCTGAGTA CATTAGGCTG GATCCATATT 
TTTGACACTG TAAAGGGCAT GCATGTAGCC CGCGTGGACT TGGCTCTTCC CCGAGAAGAT 
TCTGAGCAGC AGGAGCCTGC CAGCATTTCT TCCTTTATGT CACTGAAAGT GTCTCGGGAC 
TTAGATGTTT TGCTACTCGT CAGCTCGTCC AGTACTGCAG TTGCTCTTAA CCTTCACTTG 
TATTTCAGGC TACACCCAGG GCACCTGCTG TGTGAAGGAA CTCTGCAAGA CCTCCCTATT 
GAAGGGCCTA CAGGAATAGA TGAAGATGAC CTTGTTAATT CTGCCCACAA CATGAAACTG 
ACCAAGGTGT CCTTCCAAGT TGATAGGTCC TGGAGAGCCC AGCTGGCATC ACTGAATGAA 
TCTGTGAGGA GCTCAGAACC AGAGCTTTCC TGCTATCCTC CGTGGTTCCA GGGTGCTTTG 
TGTTTGGAGT CCCTGGAATC TGGTAACCAC TCCCCCATTG TGCCAAACCA CGTTTTCATT 
CCAGGGGATG TCCCGAGTGG CCAGTATGCT TTTCCTCAGA AGGAGCACAG CAAGACCAGT 
GAGCCAAGGC CGTGGAGGAC AATGCACCTG AGTGAACAAG AGCAGCCCAC AGAGCTCACG 
TGTCTGTCTG TGACGGGATT TACGGCCCTG TTTATTCAGG CAGTAGGAAC GACCAGCTGC 
ACCGTCGTCC TCTGGGACTT GGAGACCCAG AGCATGCAGT GTTTTTCTCT CAGCCAGAAG 
TGTACTCCTG TAGACATCAG CGGAGACCAG CAGCTGTGCC TTGCTTTGAC AGAGAACGGC 
CTGTCTCTGA TTTTGTTTGG TTTAACTCAA GAGGAGTTTC TGAACAGACT GATGATCCAT 
GGCAGTGCCA GCACTGTGGA CTCCCTTTGT CATCTCAACG GTTGGGGACG GTGCTCAATC 
CCCATCCACG CTCTAGAGGC TGGCATAGAA AATCGTCAGC TGGACACGGT AGATTTCTTC 
CTGAAGAGCA AGGAAAATCT TCTTACTCCA TCTTCAAAAT CTCCTGCCCC TGATCAGCAG 
CAGCACTTCT CCTCCCAGCT CTACCTAAGG CAGGTGGAAG AGATGACCCC AGCCCTGGAC 
TTACTCTGCT CAGCAATTAG AGAAAGCGAC TCCGAAACCC AAAGCAAGCA CTTTGCAGAG 
CAGCTGCTTC ACCTCACACT GTCTTTCCTT AACAAGCAAA TGAGGGAGCT TTTTGATCAC 
ACTGAAGAGC TAGATGAGCA TTTACAGAAA GGAGCAGCCA TTTTGACCAG CTACATCAAT 
GAACTTCGGA CTTTTATGAT AAAGTTTCCT TGGAAGCCAG GAGATGCCAT AGATGAATCT 
GATGAAAATG AAGAGGTCCT CACAGTGAAA GAAGGCCAGG TTTGGGAGGA ACTCCGCTCT 
GAGGAAGTTA TTGCTGATGC CATTTTAAAT AACAGAATAC CAGAGGCACA AACTTTCTTC 
AGGATTACTG GTCATTCCGC TCAACGACTT GAGGAACTGG TTAGGATAGG CCTAGATTTG 
GCATTTGACA GTTTAAAAAA GAACAGCGTG GAGGAGGCCT CCAGGCTCTT GAGGAACATG 
GGCTTCAGTG TGGAAGACGA GCTGCTTAAG ATAAGCTTTT ATACGACTGA TAAAAACATA 
CGGGACTTTT TGGTTGAAAT TTTAAAAGAA AAGGAATGTT TTTCTGAAAA AGAAAGAAGA 
ACTGTGGACT TTGTCCATCA AGCTGAAAGG CTTTACTCAG GACACTTCCA GGAAAATGCG 
CCGATGCAGG CTTTTCCCAG GTATTGGATA AAGGAACAGG ACTGTTTCAA ACACAAATCT 
GTCTTGGACA CGTTCCTCAA ATGTGATACA AAAGATGAGT TTACCAAACA GGACCATAGA 
ATTGCACTGA ATTGGGCTCA CTGGTGGGAT CGGCAAACAC AGGAGTGTAT CCTTCTCCCC 
AGAATAAGTT CAGAAGAATA TAAAGCACAT TCCCCAGAAG CCCTGTGGAG ACACCTCACA 
GCTCGCCATG ACTGGTCAAG CATCAGCTTG TGGATGGAAG AATTTCAGGC CCAGGAAACT 
GTGTCACCTC AGCAGAGCAA GTGGCCTCCT CTGAGTGCTG CCGTCATCGA GCAGAACACT 
TGCTGCAGCA GGCACATGAG GAACGCGATC TTAGACCAGC TGGCCAGATG TGGCATTTTT 
CTTGCATCTG AGCTGGAAGA CTTTGAACTT CTCCTCCTGA GACTGAGCCG GATTGGGGGT 
GTAATGCAGG ATGCCCTCCC CGTGCAGAGC TACAGATCCT CGGCAGGGTG CAACTTTGAT 
TCCCAAGTCA TCCTCTACTG TTTAGAGCAC GGCCTGCAGC ATCTTCTCTA CGTCTACCTT 
GACTATTACA AACTTAGCCC TGGAAATTGT CCCTTTTTGG AAAAGAAAGA ACTACATGAA 
GCTCACCCTT GGCTTGAATT TTTGGTTCAG TGTCGGCAAG TTTCCAGTAA CTTGACAGAT 
CCCAAACTGA TCTTTCAGGC CAGTCTTGCA AATGCTCAGA TTTCGATTCC CACCAATCAA 
GCCAGTGTGA GCAGCATGCT GTTGGAAGGA CACACCCTCT TGGCTCTTGC TACCACCATG 
TATGCTCCTG GGGGCGTCAG CCAGGTCATT CAGCATGAAG ACAGCGAGAA CTGTTTGAAG 
AAAGTGGACC CCCAGCTACT GAAGATGGCC TTAACGCCTT ACCCAAAGCT CAAAGCCGCG 
CTCTTCCCTC AGTGCACTGC TCCTAGTATT CTGCCCTCTG ACATTACACT CTACCACCTC 
ATCCAGTCCT TACCACCCTT TGATCCCAGC AGACTGTTTG GCTGGCAGTC TGCCAACACT 
CTAGCTATCG GAGATGCGGC CGGTCAGCTC CCACACTTCT CCAGTCCTGA CCTGGTGAGT 
AAATACGCCG TGGTGGAGCA GCTGAGCTAC ACGTATTACT TACATCACGG ACGCCCATCC 
TTCGCCTTCG GCACCTTTCT GGTCCAGGAG TTAATCAAGA GTAAGACTCC CAAGCAGCTG 
ATCCAGCAAG TAGGCAAGGA GGCCTATACC TTAGGGCTCT CCTCCTTCCA CAACCCTTCG 
GTAGGGGCCG CCTGTGTCTG CTTCTTAGAA TTGCTTGGCC TCAGCAGCCT CAAGCTCAGA 
GTTGACCTGA AAATGGCAAA CGTAGTTCTG GGCTCCAGGC GTAGAAATGA AGATGCACAT 
CCCAGCTTCA TCAGAGAGTC TCTGGCTGAG AAGCTGTCTA AGCTTGCTGA CGGTGACAGG 
GCAACCACAG AAGAGCTGCT CGTTCTCTTG GAGGAAGGCG TGTGGGACAG CATTGAGCAG 
CAGGGCTTTA ACAGGTTGTC CAGCGAATCC AGCAGCCAGT GGTCATTAGT GTTACAGTTC 
TGCATGCTCC ATGATATGAA ACTGAGCGTG TCCTACCTTA GAGAATGTGC CAAAGCAAAT 
GATTGGCTGC AGTTTCTGGT TCACAGCCAG CTCCATAACT ACCACCCAGA AGAGGTGGAA 
ACCCTGCTTC AGTATTTCAG TCCTGTCCTT CAGAGTCACT TGAAGCTGGC TTCTGAGAAA 
CTGTCATCAG GGTCCATCTC TAAGGACGAC AGCTGCCTCC AGGAACTCCA GAAAAACAAA 
GAAACAAGTG ATTTCTTTGA AATTCTCCAC CGGTGCTCGG ATGAGTCAGC TTCCTGGTGC 
TGGCTCCTGG CTGAAGCAGT GAGACATCGG GTTCCCATCC TCAGTGTCCT GGCCTCGTGT 
ATCCAGGGTG CCAGTGCTGT CCCCTGTCTC TGTGTTTGGA TTGTCACCTC TGTGGAGGAC 
AAGGTTGCAG CTGAAGCAAT AGAACACATT CAGATCTCTG TAGAGGACCA TCATTGGAGC 
CTCGAGGACC TGTCCATTAT CTGGAGAACT GTATTGACAA GACGGAAGAT CCACACTCTC 
ATCCGAGGCT TCCGGCTTTT CATTAAGGAT TCCCCACTTC TCTTGATTAT GGAAATGTAT 
GAACTGTGTG TGTTCTTCAA GAACTACGGA AAAGCCAAAG TTAAGCTTGT GGAGTTCCAG 
AAAAGCCTTG AAATGCTTGA CACAGTGGCT ACTCGGGTCC TTCCTGTCAT ACCTGCCACG 
TGGATGAGGG AGCAGGTGTG CTTCCTTTTG AAACTTATGC CACAGCAGTG CGAGACACAG 
TATGAGCTGC AGAAAGTCCT CCAACTGTTT GTCGGGATGG AGCAGCTCTT CTCCGATGGG 
CCAGACGTGC AGAAGCTGTA TCGGCTCAGC CAAGTACTGA AGGACACACC TATCACCATC 
AGCCCTGCAG TCATTTCCAG CTACAGCACT GAGAGTTTGC AGCGTGAGTG TAGATCTATT 
TTGGAGAAGC TAAAGGCAGA TGGACAGTTT GCTGTGGCCA GGAGAGTGGC AGAATTAGCA 
TCCCTGCCTG TGGACGGCTT GGTGATTGAA CAGTTAACGC AGGAAATGCA AATGCTAACA 
CACACCCAGC AGTGGTCCCT GAAACAAGCA AGACTTGACT TCTGGAGAAA ATGCCATGAA 
AATTTTAAGA AAAACTCTGT TTCAAACAGA GCAGCCTCTT CCTTCTTCTC CAGCCAAGCC 
CCGGAGGCGA GTGAGCACCC TGCTGAGCAG GGCAGCCTTG AGGAGCGCCA TCTGCTGCTC 
ACTCTGGCGG GCCACTGGCT TGCCCAAGAA GAGCCAGTTC CCGTGGAGGA ACTGGAGGAG 
CTGGAGAAAC AGATTTGGCT CTGCCGCATC GCCCAGCACA CATGTGGAGG GGCTGAAGAA 
GAAACCAAGC CCAGCCTCTC GCAACACAAG TTGGCCCCTG CTGAACTTTC TTCTGATAGC 
TCAGCCAGTG AGTTCTCCTT CTCCAAGCTT GCTGCTTTGA ACACATCCAA ATACTTAGAC 
CTTAATGGTC TTCCATCCAA GGAGATGTGT GAGAACACAC TGGAACACAG AGAGCAGGAG 
TCACTGAACA CTTTGATTGG GCAGCTGCTG GATGGTGGCT GTGTGCATGA AGCGAGCAGA 
GTGTGCCGGT ATTTCCGTGT CTATAGTCAG GATCTAGTGC TGCTGCTGCA CTGCAGAGCC 
TTGGCCTCCG CAGAGGCCAG CATGGAAGAT CTGCACTCAG AGATCCGTGC CCTCCTCTCA 
AATGCCACAC TACCTGAGGA CCTGGAGAGC CCTGGCGTCC CTCTTCGGAA AGTCCATAGC 
ACTTCGAGTT TGGACAGTCT GTCATTTGCG ATGGTACCAC CTACTGATGA AGTGGCCAGG 
AGTCTCCAAA CTCTCATAAG CAGGTGCCTC CATGGGAAGG ATTACTGTCG GCAGGTCCTC 
TGTCTGTATG AACTTGCCAA GGAGTTGGGC TGTTCCTATG GAGATGTTGC TACCAGGGAC 
AGTGAAGCCA TGCTCCGAGC AATCTTGGCT TCTCAGCGGC CTGACCGATG CAGACAAGCC 
CAGGTCTTCA TCAACACCCA GGGCCTTGAA CCAGACACTG TGGCTGAACT TGTGGCTGAA 
GAGGTGACAC GAGAGCTACT GACCCCATCA GAGGGAGCAG GAGAAAAGCA GCCTTTCAAC 
CTAGCAGAGG AAAGCCAGAC ATTTCTTCAG CTAACTGCTT TGTGTCAAGA CCGTACATTG 
GTAGGCATGA AGTTGTTGGA CAAGATTCCA TCTGTCCCCC ATGGGGAGCT GTCATGCACC 
ACAGAGCTCC TGATCCTGGC CCATCACTGC TTTACCTGTG CGTGTTACAT GGAGGGCATC 
ATCAGAGTCC TACAGGCTGC CCGGATGCTT ACAGATAACC ACTTGGCTCC CAATGAGGAG 
TATGGATTGG TGGTACGCCT CCTCACTGGC ATTGGACGGT ACAATGAGAT GACGTACATA 
TTCGACTTGC TGCATCAAAA GCATTATTTT GAAGTTCTGA TGAGGAAGAA GCTAGATCCA 
ACCGGCACCC TGAAAACAGC CCTACTAGAC TACATCAAAC GCTGCCACCC TGGAGACAGT 
GAAAAGCACA ATATGATCGC CCTGTGCTTC AGCATGTGTC GGGAGATAGG AGAGAACCAC 
GAGGCCGCAG CCTGCATCCA ACTGAAGCTG ATCGAATCTC AGCCCTGGGA GGAAAGTCTC 
AAGGATGGAG CCCAACTAAA ACAGCTCCTG CTTAAAGCCC TGACTTTGAT GCTGGACGCA 
GCAGAGAGTT ACGCAAAGGA CTCCTGTGTG CGGCAGGCCC TGCATTGTAA CCGGCTCACC 
AAGTTGATAA CACTACAGAT TCACTTTCTC AACAGTGGCC AGAACACAAT GCTCATCAAC 
TTGGGCCATC AGAAGCTAAT GGACTGTATC ATGACCCTAC CTCGGTTCTA CCAGGCTTCT 
ATTGTAGCTG AGGCCTATGA CTTTGTTCCT GATTGGGCTG AAGTACTCTA CCAACAAGTG 
ATTCTTAAAG GAGACTTTAG TTACTTGGAA GAATTTAAGC AGCAGAAGTT ACTAAAACCC 
AATATATTTG AAGACATTTC CAAAAAATAT AAACAACACC AGCCTACTGA CAGGGTCACA 
GAAAACCTGA AGAAGTTACT TTCATATTGT GAAGACATCT ACCTGTATTA CAAGTTGGCA 
TATGAACACA AGTTTTTTGA AATTGTAAAT ATGCTTCTGA AGGATCCTCA AACAGGATGC 
TGTCTTAAAG ATATGCTCGC GGGTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

In Vivo Evidence for Lysosome Depletion and Impaired Autophagic Clearance in Hereditary Spastic Paraplegia Type SPG11.
PLoS genetics11(8)e1005454(2015 Aug)
Varga RE,Khundadze M,Damme M,Nietzsche S,Hoffmann B,Stauber T,Koch N,Hennings JC,Franzka P,Huebner AK,Kessels MM,Biskup C,Jentsch TJ,Qualmann B,Braulke T,Kurth I,Beetz C,Hübner CA

Dysfunction of spatacsin leads to axonal pathology in SPG11-linked hereditary spastic paraplegia.
Human molecular genetics23(18)4859-74(2014 Sep)
Pérez-Brangulí F,Mishra HK,Prots I,Havlicek S,Kohl Z,Saul D,Rummel C,Dorca-Arevalo J,Regensburger M,Graef D,Sock E,Blasi J,Groemer TW,Schlötzer-Schrehardt U,Winkler J,Winner B

Cellular distribution and subcellular localization of spatacsin and spastizin, two proteins involved in hereditary spastic paraplegia.
Molecular and cellular neurosciences47(3)191-202(2011 Jul)
Murmu RP,Martin E,Rastetter A,Esteves T,Muriel MP,El Hachimi KH,Denora PS,Dauphin A,Fernandez JC,Duyckaerts C,Brice A,Darios F,Stevanin G

Integral and associated lysosomal membrane proteins.
Traffic (Copenhagen, Denmark)8(12)1676-86(2007 Dec)
Schröder B,Wrocklage C,Pan C,Jäger R,Kösters B,Schäfer H,Elsässer HP,Mann M,Hasilik A

Mutations in SPG11, encoding spatacsin, are a major cause of spastic paraplegia with thin corpus callosum.
Nature genetics39(3)366-72(2007 Mar)
Stevanin G,Santorelli FM,Azzedine H,Coutinho P,Chomilier J,Denora PS,Martin E,Ouvrard-Hernandez AM,Tessa A,Bouslam N,Lossos A,Charles P,Loureiro JL,Elleuch N,Confavreux C,Cruz VT,Ruberg M,Leguern E,Grid D,Tazir M,Fontaine B,Filla A,Bertini E,Durr A,Brice A

REQUEST A QUOTE

EMAIL

gene@genscript.com

PHONE

1-877-436-7274

ONLINE FORM

Online Quote Submission

ONLINE FORM

Online Quote Submission

Spg11 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Most Popular Services

Services & Products

News & Blogs

REQUEST A QUOTE

EMAIL

PHONE

ONLINE FORM

ONLINE FORM