AFF4 cDNA ORF clone, Ornithorhynchus anatinus(platypus)

Gene
Clones
gRNAs

Summary

Gene Symbol	AFF4
Entrez Gene ID	100079379
Full Name	AF4/FMR2 family member 4
Gene Type	protein-coding
Organism	Ornithorhynchus anatinus(platypus)

mRNA and Protein(s)

mRNA	Protein	Name
XM_029051767.1	XP_028907600.1	AF4/FMR2 family member 4 isoform X1
XM_029051768.1	XP_028907601.1	AF4/FMR2 family member 4 isoform X1
XM_029051769.1	XP_028907602.1	AF4/FMR2 family member 4 isoform X1
XM_029051770.1	XP_028907603.1	AF4/FMR2 family member 4 isoform X2
XM_007670296.1	XP_007668486.1	AF4/FMR2 family member 4

Pathways from BioSystems
Gene Ontology

The following AFF4 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the AFF4 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OOc136284	XM_029051770.1 Latest version!	Ornithorhynchus anatinus AF4/FMR2 family member 4 (AFF4), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
OOc12461	XM_007670296.1 Latest version!	Ornithorhynchus anatinus AF4/FMR2 family member 4 (AFF4), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OOc136283	XM_029051768.1 Latest version!	Ornithorhynchus anatinus AF4/FMR2 family member 4 (AFF4), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OOc136283	XM_029051767.1 Latest version!	Ornithorhynchus anatinus AF4/FMR2 family member 4 (AFF4), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OOc136283	XM_029051769.1 Latest version!	Ornithorhynchus anatinus AF4/FMR2 family member 4 (AFF4), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

AFF4 ( XM_029051770.1 ) cDNA ORF clone, Ornithorhynchus anatinus(platypus) -> XP_028907603.1 Ornithorhynchus anatinus AF4/FMR2 family member 4 (AFF4), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OOc136284
Clone ID Related Accession (Same CDS sequence)	XM_029051770.1
Accession Version	XM_029051770.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2538bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1556380800000
Organism	Ornithorhynchus anatinus(platypus)
Product	AF4/FMR2 family member 4 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_041749.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Ornithorhynchus anatinus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCACCC CCTCTAACAG CCATTCATAC GCCATGCAAA ACAGAGCCTT CCAAATTTCC 
TTTTCCAACC AAGGAGTCTC AGCAATCCAA TTTTGGTACT GGAGAACAAA AAAAAGATAC 
AATCCTCCAT CTAAAACATC AAATGGTCAC CAGGCCAAAT CATGTGAATC AAACCAGACA 
GATATGTTAA AAGATGACTT AAAACTCAGC AGCAGTGAAG ACAGTGATGG GGAACAGGAT 
TGTGATAAAA CAGTGCCAAG GAGTACCCCT GGAAGTAATT CTGAACCGTC ACACCATAAT 
AGTGAAGGAG CAGATAATTC CAGGGATGAC TCGAGCAGCC ATAGTGGATC CGAAAGCAGT 
TCCGGATCTG ACTCGGAAAG CGAGAGTAGT TCCAGTGACA GCGAGGCTAA CGAGCCATCC 
CAGAGTGCGT CTCCCGAGCC CGAGCCACCC CCAACCAACA AATGGCAACT GGACAATTGG 
TTGAATAAGG TGAATCCACA CAAAGTGTCA CCTGCTTCCT CTGTGGACAG CAACATCCCC 
TCCTCACAAG GTTATAAGAA GGAGGGTCGG GAGCAATGCC CTGGGAGTGG CTACGTCGAT 
CAGACAGGGC CTAAGGATTC CAACTCCTCC ACTCCCGGCC GAGATTCCAA AACCGTCCAA 
AAAGGATCCG AGGGTAGTCG CGGCAGACAA AAGTCTCCCG CCCAGAGTGA TAACACGACC 
CAAAGAAGAA CTGTGGGGAA GAAGCAGCCC AAAAAGGCAG AGAAAGCAGC TGTTGAGGAA 
CCTAGGGGAG GACTAAAAAT AGAAAGTGAA ACCCCCCTGG ATATGGCCAC AAGCATGCCC 
TCAAACAGGC ACAAGGCAGC CACAAAAGGC TCCAGGAAGC CCAACATAAA GAAGGAACCC 
AAGTCATCCC CTAGGCCCAC GACGGAGAAA AAGAAATACA AGTCAACAAG CAAATCTTCC 
CAGAAATCTC GGGAAATCAT AGAAACAGAT ACCTCATCCT CTGATTCTGA TGAAAGCGAG 
AGCCTCCCGC CTTCATCACA AACTCCTAAA TATCCCGAGA GCAATAGGAC TCCTGTTAAA 
CCCTCCTCCA TGGAGGAGGA AGACAGCTTT TTTCGGCAGA GAATGTTCTC TCCTATGGAA 
GAGAAGGAAC TCCTTTCACC ACTCAGTGAG CCTGATGACA GGTATCCTCT CATTGTGAAG 
ATTGACCTGA ATCTCTTGTC CAGAATACCA GGGAAGCCTT ACAAAGAAAC TGAGCCACCT 
AAGGTGGAAA AGAAAACTGT GCCAGAAAAG CACCCGAGAG ACCCACAGAA ACAAGCCTCA 
GACAAAGTCT CTAACAAGGG CAAGAGGAAA CATAAGAATG ATGATGACAA TAGACCCAGT 
GAAAGCAAGA AAACTAAACT GGAGGAAAAG GGTTCACTGA GCCACAAGAC ATCCAGCAAT 
AGAGAGTCAT CCAAACCAAG TGCTATAAAA GAGAAGGATC TTTTGCCTTC CCCAGCTGCA 
CCTATTCCCC CAAAAGATCC AAAACCAGAG CACGGGTCCC GGAAGAGGAC AGTCAGCCAA 
TCGTCTTCCT TAAAATCTAG CAGCAACAGT ATCAAGGAGA GCAGCAGCAG TAAAAACAGT 
TCTTCCTCTT CAAAGCAAAA AAAGACAGAA GGAAAGGTTT CTACCAGTTC CAAGGAAACC 
AAGGAAAAAA ATCCAAACAA CTCTTCAAAT TGTCCTCCAT CTACAACAAC TCCTGACAGT 
TCCAAGTCCC GAAGAGCAAA GCTTGCTTTT GATGACAGGA GCTATTCAGC AGATCATTAC 
TTACAAGAAG CAAAAAAACT AAAGCACAAT GCAGATGCGT TGTCCGATAG GTTTGAGAAA 
GCGGTCTTCT ACCTTGATGC TGTTGTGTCT TTCATTGAAT GTGGGAATGC ATTGGAGAAA 
AACGCTCAGG AATCCAAATC CCCGTTCCCT ATGTATTCAG AAACTGTGGA ACTAATTAAA 
TACACTATGA AGCTGAAGAA TTACTTGGCA CCAGATGCCA CAGCTGCAGA TAAAAGACTA 
GCCGTGCTAT GTCTGCGATG CCAGTCCCTG TTGTACCTGA GGTTGTTCAA GCTGAAGAAA 
GAGAGTGCGT TGAAATACTC AAAAACACTG ACGGAACATC TAAAGAACTC CTATAATAAT 
TCGCAAGCTC CCTCACCTGG CATAGGAAGC AAAGCCGCCG GGATGCCCTC GCCTGTCTCC 
CCCAAGCTTT CTCCGGGCAA TTCAGGAAAC TACTCCTCAG GGGCCAGCAA CCCTTCGGGG 
AGCGGTTCTT CGGTGACAAT TCCGCAGAGG ATCCACCAGA TGGCGGCCAG CTACGTCCAG 
GTCACCTCCA ACTTCCTCTA CGCGACTGAA ATTTGGGACC AAGCCGAACA GCTTTCCAAA 
GAGCAAAAAG AATTCTTTGC TGAACTGGAT AAAGTTATGG GCCCGCTCAT CTTTAATTCA 
AGCATCATGA CAGACCTAGT TCGTTACACC CGGCAGGGAC TCCACTGGTT GCGTCAGGAT 
GCCAAGTCGA TACCTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_028907603.1
CDS	104..2641
Translation	MATPSNSHSYAMQNRAFQISFSNQGVSAIQFWYWRTKKRYNPPSKTSNGHQAKSCESNQTDMLKDDLKLSSSEDSDGEQDCDKTVPRSTPGSNSEPSHHNSEGADNSRDDSSSHSGSESSSGSDSESESSSSDSEANEPSQSASPEPEPPPTNKWQLDNWLNKVNPHKVSPASSVDSNIPSSQGYKKEGREQCPGSGYVDQTGPKDSNSSTPGRDSKTVQKGSEGSRGRQKSPAQSDNTTQRRTVGKKQPKKAEKAAVEEPRGGLKIESETPLDMATSMPSNRHKAATKGSRKPNIKKEPKSSPRPTTEKKKYKSTSKSSQKSREIIETDTSSSDSDESESLPPSSQTPKYPESNRTPVKPSSMEEEDSFFRQRMFSPMEEKELLSPLSEPDDRYPLIVKIDLNLLSRIPGKPYKETEPPKVEKKTVPEKHPRDPQKQASDKVSNKGKRKHKNDDDNRPSESKKTKLEEKGSLSHKTSSNRESSKPSAIKEKDLLPSPAAPIPPKDPKPEHGSRKRTVSQSSSLKSSSNSIKESSSSKNSSSSSKQKKTEGKVSTSSKETKEKNPNNSSNCPPSTTTPDSSKSRRAKLAFDDRSYSADHYLQEAKKLKHNADALSDRFEKAVFYLDAVVSFIECGNALEKNAQESKSPFPMYSETVELIKYTMKLKNYLAPDATAADKRLAVLCLRCQSLLYLRLFKLKKESALKYSKTLTEHLKNSYNNSQAPSPGIGSKAAGMPSPVSPKLSPGNSGNYSSGASNPSGSGSSVTIPQRIHQMAASYVQVTSNFLYATEIWDQAEQLSKEQKEFFAELDKVMGPLIFNSSIMTDLVRYTRQGLHWLRQDAKSIP

Target ORF information:

RefSeq Version	XM_029051770.1
Organism	Ornithorhynchus anatinus(platypus)
Definition	Ornithorhynchus anatinus AF4/FMR2 family member 4 (AFF4), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_029051770.1

ORF Insert Sequence:

ATGGCCACCC CCTCTAACAG CCATTCATAC GCCATGCAAA ACAGAGCCTT CCAAATTTCC 
TTTTCCAACC AAGGAGTCTC AGCAATCCAA TTTTGGTACT GGAGAACAAA AAAAAGATAC 
AATCCTCCAT CTAAAACATC AAATGGTCAC CAGGCCAAAT CATGTGAATC AAACCAGACA 
GATATGTTAA AAGATGACTT AAAACTCAGC AGCAGTGAAG ACAGTGATGG GGAACAGGAT 
TGTGATAAAA CAGTGCCAAG GAGTACCCCT GGAAGTAATT CTGAACCGTC ACACCATAAT 
AGTGAAGGAG CAGATAATTC CAGGGATGAC TCGAGCAGCC ATAGTGGATC CGAAAGCAGT 
TCCGGATCTG ACTCGGAAAG CGAGAGTAGT TCCAGTGACA GCGAGGCTAA CGAGCCATCC 
CAGAGTGCGT CTCCCGAGCC CGAGCCACCC CCAACCAACA AATGGCAACT GGACAATTGG 
TTGAATAAGG TGAATCCACA CAAAGTGTCA CCTGCTTCCT CTGTGGACAG CAACATCCCC 
TCCTCACAAG GTTATAAGAA GGAGGGTCGG GAGCAATGCC CTGGGAGTGG CTACGTCGAT 
CAGACAGGGC CTAAGGATTC CAACTCCTCC ACTCCCGGCC GAGATTCCAA AACCGTCCAA 
AAAGGATCCG AGGGTAGTCG CGGCAGACAA AAGTCTCCCG CCCAGAGTGA TAACACGACC 
CAAAGAAGAA CTGTGGGGAA GAAGCAGCCC AAAAAGGCAG AGAAAGCAGC TGTTGAGGAA 
CCTAGGGGAG GACTAAAAAT AGAAAGTGAA ACCCCCCTGG ATATGGCCAC AAGCATGCCC 
TCAAACAGGC ACAAGGCAGC CACAAAAGGC TCCAGGAAGC CCAACATAAA GAAGGAACCC 
AAGTCATCCC CTAGGCCCAC GACGGAGAAA AAGAAATACA AGTCAACAAG CAAATCTTCC 
CAGAAATCTC GGGAAATCAT AGAAACAGAT ACCTCATCCT CTGATTCTGA TGAAAGCGAG 
AGCCTCCCGC CTTCATCACA AACTCCTAAA TATCCCGAGA GCAATAGGAC TCCTGTTAAA 
CCCTCCTCCA TGGAGGAGGA AGACAGCTTT TTTCGGCAGA GAATGTTCTC TCCTATGGAA 
GAGAAGGAAC TCCTTTCACC ACTCAGTGAG CCTGATGACA GGTATCCTCT CATTGTGAAG 
ATTGACCTGA ATCTCTTGTC CAGAATACCA GGGAAGCCTT ACAAAGAAAC TGAGCCACCT 
AAGGTGGAAA AGAAAACTGT GCCAGAAAAG CACCCGAGAG ACCCACAGAA ACAAGCCTCA 
GACAAAGTCT CTAACAAGGG CAAGAGGAAA CATAAGAATG ATGATGACAA TAGACCCAGT 
GAAAGCAAGA AAACTAAACT GGAGGAAAAG GGTTCACTGA GCCACAAGAC ATCCAGCAAT 
AGAGAGTCAT CCAAACCAAG TGCTATAAAA GAGAAGGATC TTTTGCCTTC CCCAGCTGCA 
CCTATTCCCC CAAAAGATCC AAAACCAGAG CACGGGTCCC GGAAGAGGAC AGTCAGCCAA 
TCGTCTTCCT TAAAATCTAG CAGCAACAGT ATCAAGGAGA GCAGCAGCAG TAAAAACAGT 
TCTTCCTCTT CAAAGCAAAA AAAGACAGAA GGAAAGGTTT CTACCAGTTC CAAGGAAACC 
AAGGAAAAAA ATCCAAACAA CTCTTCAAAT TGTCCTCCAT CTACAACAAC TCCTGACAGT 
TCCAAGTCCC GAAGAGCAAA GCTTGCTTTT GATGACAGGA GCTATTCAGC AGATCATTAC 
TTACAAGAAG CAAAAAAACT AAAGCACAAT GCAGATGCGT TGTCCGATAG GTTTGAGAAA 
GCGGTCTTCT ACCTTGATGC TGTTGTGTCT TTCATTGAAT GTGGGAATGC ATTGGAGAAA 
AACGCTCAGG AATCCAAATC CCCGTTCCCT ATGTATTCAG AAACTGTGGA ACTAATTAAA 
TACACTATGA AGCTGAAGAA TTACTTGGCA CCAGATGCCA CAGCTGCAGA TAAAAGACTA 
GCCGTGCTAT GTCTGCGATG CCAGTCCCTG TTGTACCTGA GGTTGTTCAA GCTGAAGAAA 
GAGAGTGCGT TGAAATACTC AAAAACACTG ACGGAACATC TAAAGAACTC CTATAATAAT 
TCGCAAGCTC CCTCACCTGG CATAGGAAGC AAAGCCGCCG GGATGCCCTC GCCTGTCTCC 
CCCAAGCTTT CTCCGGGCAA TTCAGGAAAC TACTCCTCAG GGGCCAGCAA CCCTTCGGGG 
AGCGGTTCTT CGGTGACAAT TCCGCAGAGG ATCCACCAGA TGGCGGCCAG CTACGTCCAG 
GTCACCTCCA ACTTCCTCTA CGCGACTGAA ATTTGGGACC AAGCCGAACA GCTTTCCAAA 
GAGCAAAAAG AATTCTTTGC TGAACTGGAT AAAGTTATGG GCCCGCTCAT CTTTAATTCA 
AGCATCATGA CAGACCTAGT TCGTTACACC CGGCAGGGAC TCCACTGGTT GCGTCAGGAT 
GCCAAGTCGA TACCTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

AFF4 ( XM_007670296.1 ) cDNA ORF clone, Ornithorhynchus anatinus(platypus) -> XP_007668486.1 Ornithorhynchus anatinus AF4/FMR2 family member 4 (AFF4), mRNA.

Price & Availability↑

CloneID	OOc12461
Clone ID Related Accession (Same CDS sequence)	XM_007670296.1
Accession Version	XM_007670296.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3648bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1460563200000
Organism	Ornithorhynchus anatinus(platypus)
Product	AF4/FMR2 family member 4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_001794391.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Ornithorhynchus anatinus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## ab initio :: 5% of CDS bases ##RefSeq-Attributes-END##

ATGAACCGTG AAGATCGGAA TGTGCTGCGT ATGAAAGAAA GGGAAAGGCG AAATCAGGAA 
ATTCAGCAGG GGGAAGAAGC CTTTCCACCT AACTCACCTC TCTTTGCTGA ACCTTATAAA 
GTTACCAGCA AGGAAGACAA ATTATCTAGT CGTATTCAGA GCATGCTCGG AAATTACGAT 
GAACAGGGCG TGCTTGGAAA CTACGATGAA ATGAAGGATT TTCGAGGAGA CCGATCTCTA 
CAGAAGCTGG TCGTGATCCC CAAGTCAACA GCGCCATCAG CAACAGATGA AAAATCCAAC 
CCAAGTTTCT TTGGTGAACA GAGACACAGT AGCTCTCATC AGAGCAGCAA ATGGACTCCT 
GTAGGGCCAG CACCTAGCAC TTCTTCCCAG TCCCAGAAAC GGTCCTCCGG TTTACAGAGT 
GGACACAGTA GCCAACGGAC CGGGGGCGGG GGCGGCGGCG GCGGCCAGAG GCATGACCGT 
GACTCTTACG GCGGCGGCGG CCGCAAAAAA ACTCAGCACG GAGCGGAACA CTCCAAATCG 
CGTTCTTCCA GCCCTGGGAA ACCCCCTGCT GTGTCTTCAT TAAGCTCCAG CCATTCCAGG 
TCTCATGGGA ATGATCATCA CAGTAAGGAA CATCAGCGCG CCAAGTCTCC TCGGGACCCC 
GAGGCAAACT GGGACTCGCC CTCCCGGGTT CCTTCATTTT CAAGCGGGCA GCACTCTAAC 
CAATCTTTCC CTCCTTCGTT GATGTCAAAG TCCAGCTCAA TGTTGCAGAA GCCCACTGCT 
TACGTAAGGC CCATGGATGG ACAGGAGTCC ATGGAACCAA AGCTATCCTC TGAGCACTAC 
AGCAGCCAAT CTCATAGCAA CAGCATGAGT GAGCTGAAGC CTAGCAGCAA AGCACACCTA 
ACCAAACTGA AAATTCCCTC TCAACCATTA GATGCATCAG TATCTGGTGA TGTGAGCTGT 
GTGGATGAAA TTCTCAAAGA GATGACTCAC TCATGGCCAC CCCCTCTAAC AGCCATTCAT 
ACGCCATGCA AAACAGAGCC TTCCAAATTT CCTTTTCCAA CCAAGGAGTC TCAGCAATCC 
AATTTTGGTA CTGGAGAACA AAAAAGATAC AATCCTCCAT CTAAAACATC AAATGGTCAC 
CAGGCCAAAT CATGTGAATC AAACCAGACA GATATGTTAA AAGATGACTT AAAACTCAGC 
AGCAGTGAAG ACAGTGATGG GGAACAGGAT TGTGATAAAA CAGTGCCAAG GAGTACCCCT 
GGAAGTAATT CTGAACCGTC ACACCATAAT AGTGAAGGAG CAGATAATTC CAGGGATGAC 
TCGAGCAGCC ATAGTGGATC CGAAAGCAGT TCCGGATCTG ACTCGGAAAG CGAGAGTAGT 
TCCAGTGACA GCGAGGCTAA CGAGCCATCC CAGAGTGCGT CTCCCGAGCC CGAGCCACCC 
CCAACCAACA AATGGCAACT GGACAATTGG TTGAATAAGG TGAATCCACA CAAAGTGTCA 
CCTGCTTCCT CTGTGGACAG CAACATCCCC TCCTCACAAG GTTATAAGAA GGAGGGTCGG 
GAGCAATGCC CTGGGAGTGG CTACGTCGAT CAGACAGGGC CTAAGGATTC CAACTCCTCC 
ACTCCCGGCC GAGATTCCAA AACCGTCCAA AAAGGATCCG AGGGTGGTCG CGGCAGACAA 
AAGTCTCCCG CCCAGAGTGA TAACACGACC CAAAGAAGAA CTGTGGGGAA GAAGCAGCCC 
AAAAAGGCAG AGAAAGCAGC TGTTGAGGAA CCTAGGGGAG GACTAAAAAT AGAAAGTGAA 
ACCCCCCTGG ATATGGCCAC AAGCATGCCC TCAAACAGGC ACAAGGCAGC CACAAAAGGC 
TCCAGGAAGC CCAACATAAA GAAGGAACCC AAGTCATCCC CTAGGCCCAC GACGGAGAAA 
AAGAAATACA AGTCAACAAG CAAATCTTCC CAGAAATCTC GGGAAATCAT AGAAACAGAT 
ACCTCATCCT CTGATTCTGA TGAAAGCGAG AGCCTCCCGC CTTCATCACA AACTCCTAAA 
TATCCCGAGA GCAATAGGAC TCCTGTTAAA CCCTCCTCCA TGGAGGAGGA AGACAGCTTT 
TTTCGGCAGA GAATGTTCTC TCCTATGGAA GAGAAGGAAC TCCTTTCACC ACTCAGTGAG 
CCTGACGACA GGTATCCTCT CATTGTGAAG ATTGACCTGA ATCTCTTGTC CAGAATACCA 
GGGAAGCCTT ACAAAGAAAC TGAGCCACCT AAGGTGGAAA AGAAAACTGT GCCAGAAAAG 
CACCCGAGAG ACCCACAGAA ACAAGCCTCA GACAAAGTCT CTAACAAGGG CAAGAGGAAA 
CATAAGAGAA AAGCTAGACT CCAGCCCCTA CTTCAGTGGG TACCATTAAT GGTAGAGGAC 
TTTCCATTCC CCTTTGACCC TCCTGCGATG TTCTGCTTGG AGAGTTTAGT CTTCCAGCTG 
GCGGCCCTGA GTCTCTTCCA GGAGCTCACA GATGATTCAT GGATGCTCTG GTACATCGCT 
CGCTTTATGG CGGCCAAGCA AAATTCAGAA TTACAGTCAT CCAAACCAAG TGCTATAAAA 
GAGAAGGATC TTTTGCCTTC CCCAGCTGCA CCTATTCCCC CAAAAGATCC AAAACCAGAG 
CACGGGTCCC GGAAGAGGAC AGTCAGCCAA TCGTCTTCCT TAAAATCTAG CAGCAACAGT 
ATCAAGGAGA GCAGCAGCAG TAAAAACAGT TCTTCCTCTT CAAAGCAAAA AAAGACAGAA 
GGAAAGGTTT CTACCAGTTC CAAGGAAACC AAGGAAAAAA ATCCAAACAA CTCTTCAAAT 
TGTCCTCCAT CTACAACAAC TCCTGACAGT TCCAAGTCCC GAAGAGCAAA GCTTGCTTTT 
GATGACAGGA GCTATTCAGC AGATCATTAC TTACAAGAAG CAAAAAAACT AAAGCACAAT 
GCAGATGCGT TGTCCGATAG GTTTGAGAAA GCGGTCTTCT ACCTTGATGC TGTTGTGTCT 
TTCATTGAAT GTGGGAATGC ATTGGAGAAA AACGCTCAGG AATCCAAATC CCCGTTCCCT 
ATGTATTCAG AAACTGTGGA ACTAATTAAA TACACTATGA AGCTGAAGAA TTACTTGGCA 
CCAGATGCCA CAGCTGCAGA TAAAAGACTA GCCGTGCTAT GTCTGCGATG CCAGTCCCTG 
TTGTACCTGA GGTTGTTCAA GCTGAAGAAA GAGAGTGCGT TGAAATACTC AAAAACACTG 
ACGGAACATC TAAAGAACTC CTATAATAAT TCGCAAGCTC CCTCACCTGG CATAGGAAGC 
AAAGCCGCCG GGATGCCCTC GCCTGTCTCC CCCAAGCTTT CTCCGGGCAA TTCAGGAAAC 
TACTCCTCAG GGGCCAGCAA CCCTTCGGGG AGCGGTTCTT CGGTGACAAT TCCGCAGAGG 
ATCCACCAGA TGGCGGCCAG CTACGTCCAG GTCACCTCCA ACTTCCTCTA CGCGACTGAA 
ATTTGGGACC AAGCCGAACA GCTTTCCAAA GAGCAAAAAG AATTCTTTGC TGAACTGGAT 
AAAGTTATGG GCCCGCTCAT CTTTAATTCA AGCATCATGA CAGACCTAGT TCGTTACACC 
CGGCAGGGAC TCCACTGGTT GCGTCAGGAT GCCAAGTCGA TACCTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_007668486.1
CDS	351..3998
Translation	MNREDRNVLRMKERERRNQEIQQGEEAFPPNSPLFAEPYKVTSKEDKLSSRIQSMLGNYDEQGVLGNYDEMKDFRGDRSLQKLVVIPKSTAPSATDEKSNPSFFGEQRHSSSHQSSKWTPVGPAPSTSSQSQKRSSGLQSGHSSQRTGGGGGGGGQRHDRDSYGGGGRKKTQHGAEHSKSRSSSPGKPPAVSSLSSSHSRSHGNDHHSKEHQRAKSPRDPEANWDSPSRVPSFSSGQHSNQSFPPSLMSKSSSMLQKPTAYVRPMDGQESMEPKLSSEHYSSQSHSNSMSELKPSSKAHLTKLKIPSQPLDASVSGDVSCVDEILKEMTHSWPPPLTAIHTPCKTEPSKFPFPTKESQQSNFGTGEQKRYNPPSKTSNGHQAKSCESNQTDMLKDDLKLSSSEDSDGEQDCDKTVPRSTPGSNSEPSHHNSEGADNSRDDSSSHSGSESSSGSDSESESSSSDSEANEPSQSASPEPEPPPTNKWQLDNWLNKVNPHKVSPASSVDSNIPSSQGYKKEGREQCPGSGYVDQTGPKDSNSSTPGRDSKTVQKGSEGGRGRQKSPAQSDNTTQRRTVGKKQPKKAEKAAVEEPRGGLKIESETPLDMATSMPSNRHKAATKGSRKPNIKKEPKSSPRPTTEKKKYKSTSKSSQKSREIIETDTSSSDSDESESLPPSSQTPKYPESNRTPVKPSSMEEEDSFFRQRMFSPMEEKELLSPLSEPDDRYPLIVKIDLNLLSRIPGKPYKETEPPKVEKKTVPEKHPRDPQKQASDKVSNKGKRKHKRKARLQPLLQWVPLMVEDFPFPFDPPAMFCLESLVFQLAALSLFQELTDDSWMLWYIARFMAAKQNSELQSSKPSAIKEKDLLPSPAAPIPPKDPKPEHGSRKRTVSQSSSLKSSSNSIKESSSSKNSSSSSKQKKTEGKVSTSSKETKEKNPNNSSNCPPSTTTPDSSKSRRAKLAFDDRSYSADHYLQEAKKLKHNADALSDRFEKAVFYLDAVVSFIECGNALEKNAQESKSPFPMYSETVELIKYTMKLKNYLAPDATAADKRLAVLCLRCQSLLYLRLFKLKKESALKYSKTLTEHLKNSYNNSQAPSPGIGSKAAGMPSPVSPKLSPGNSGNYSSGASNPSGSGSSVTIPQRIHQMAASYVQVTSNFLYATEIWDQAEQLSKEQKEFFAELDKVMGPLIFNSSIMTDLVRYTRQGLHWLRQDAKSIP

Target ORF information:

RefSeq Version	XM_007670296.1
Organism	Ornithorhynchus anatinus(platypus)
Definition	Ornithorhynchus anatinus AF4/FMR2 family member 4 (AFF4), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_007670296.1

ORF Insert Sequence:

ATGAACCGTG AAGATCGGAA TGTGCTGCGT ATGAAAGAAA GGGAAAGGCG AAATCAGGAA 
ATTCAGCAGG GGGAAGAAGC CTTTCCACCT AACTCACCTC TCTTTGCTGA ACCTTATAAA 
GTTACCAGCA AGGAAGACAA ATTATCTAGT CGTATTCAGA GCATGCTCGG AAATTACGAT 
GAACAGGGCG TGCTTGGAAA CTACGATGAA ATGAAGGATT TTCGAGGAGA CCGATCTCTA 
CAGAAGCTGG TCGTGATCCC CAAGTCAACA GCGCCATCAG CAACAGATGA AAAATCCAAC 
CCAAGTTTCT TTGGTGAACA GAGACACAGT AGCTCTCATC AGAGCAGCAA ATGGACTCCT 
GTAGGGCCAG CACCTAGCAC TTCTTCCCAG TCCCAGAAAC GGTCCTCCGG TTTACAGAGT 
GGACACAGTA GCCAACGGAC CGGGGGCGGG GGCGGCGGCG GCGGCCAGAG GCATGACCGT 
GACTCTTACG GCGGCGGCGG CCGCAAAAAA ACTCAGCACG GAGCGGAACA CTCCAAATCG 
CGTTCTTCCA GCCCTGGGAA ACCCCCTGCT GTGTCTTCAT TAAGCTCCAG CCATTCCAGG 
TCTCATGGGA ATGATCATCA CAGTAAGGAA CATCAGCGCG CCAAGTCTCC TCGGGACCCC 
GAGGCAAACT GGGACTCGCC CTCCCGGGTT CCTTCATTTT CAAGCGGGCA GCACTCTAAC 
CAATCTTTCC CTCCTTCGTT GATGTCAAAG TCCAGCTCAA TGTTGCAGAA GCCCACTGCT 
TACGTAAGGC CCATGGATGG ACAGGAGTCC ATGGAACCAA AGCTATCCTC TGAGCACTAC 
AGCAGCCAAT CTCATAGCAA CAGCATGAGT GAGCTGAAGC CTAGCAGCAA AGCACACCTA 
ACCAAACTGA AAATTCCCTC TCAACCATTA GATGCATCAG TATCTGGTGA TGTGAGCTGT 
GTGGATGAAA TTCTCAAAGA GATGACTCAC TCATGGCCAC CCCCTCTAAC AGCCATTCAT 
ACGCCATGCA AAACAGAGCC TTCCAAATTT CCTTTTCCAA CCAAGGAGTC TCAGCAATCC 
AATTTTGGTA CTGGAGAACA AAAAAGATAC AATCCTCCAT CTAAAACATC AAATGGTCAC 
CAGGCCAAAT CATGTGAATC AAACCAGACA GATATGTTAA AAGATGACTT AAAACTCAGC 
AGCAGTGAAG ACAGTGATGG GGAACAGGAT TGTGATAAAA CAGTGCCAAG GAGTACCCCT 
GGAAGTAATT CTGAACCGTC ACACCATAAT AGTGAAGGAG CAGATAATTC CAGGGATGAC 
TCGAGCAGCC ATAGTGGATC CGAAAGCAGT TCCGGATCTG ACTCGGAAAG CGAGAGTAGT 
TCCAGTGACA GCGAGGCTAA CGAGCCATCC CAGAGTGCGT CTCCCGAGCC CGAGCCACCC 
CCAACCAACA AATGGCAACT GGACAATTGG TTGAATAAGG TGAATCCACA CAAAGTGTCA 
CCTGCTTCCT CTGTGGACAG CAACATCCCC TCCTCACAAG GTTATAAGAA GGAGGGTCGG 
GAGCAATGCC CTGGGAGTGG CTACGTCGAT CAGACAGGGC CTAAGGATTC CAACTCCTCC 
ACTCCCGGCC GAGATTCCAA AACCGTCCAA AAAGGATCCG AGGGTGGTCG CGGCAGACAA 
AAGTCTCCCG CCCAGAGTGA TAACACGACC CAAAGAAGAA CTGTGGGGAA GAAGCAGCCC 
AAAAAGGCAG AGAAAGCAGC TGTTGAGGAA CCTAGGGGAG GACTAAAAAT AGAAAGTGAA 
ACCCCCCTGG ATATGGCCAC AAGCATGCCC TCAAACAGGC ACAAGGCAGC CACAAAAGGC 
TCCAGGAAGC CCAACATAAA GAAGGAACCC AAGTCATCCC CTAGGCCCAC GACGGAGAAA 
AAGAAATACA AGTCAACAAG CAAATCTTCC CAGAAATCTC GGGAAATCAT AGAAACAGAT 
ACCTCATCCT CTGATTCTGA TGAAAGCGAG AGCCTCCCGC CTTCATCACA AACTCCTAAA 
TATCCCGAGA GCAATAGGAC TCCTGTTAAA CCCTCCTCCA TGGAGGAGGA AGACAGCTTT 
TTTCGGCAGA GAATGTTCTC TCCTATGGAA GAGAAGGAAC TCCTTTCACC ACTCAGTGAG 
CCTGACGACA GGTATCCTCT CATTGTGAAG ATTGACCTGA ATCTCTTGTC CAGAATACCA 
GGGAAGCCTT ACAAAGAAAC TGAGCCACCT AAGGTGGAAA AGAAAACTGT GCCAGAAAAG 
CACCCGAGAG ACCCACAGAA ACAAGCCTCA GACAAAGTCT CTAACAAGGG CAAGAGGAAA 
CATAAGAGAA AAGCTAGACT CCAGCCCCTA CTTCAGTGGG TACCATTAAT GGTAGAGGAC 
TTTCCATTCC CCTTTGACCC TCCTGCGATG TTCTGCTTGG AGAGTTTAGT CTTCCAGCTG 
GCGGCCCTGA GTCTCTTCCA GGAGCTCACA GATGATTCAT GGATGCTCTG GTACATCGCT 
CGCTTTATGG CGGCCAAGCA AAATTCAGAA TTACAGTCAT CCAAACCAAG TGCTATAAAA 
GAGAAGGATC TTTTGCCTTC CCCAGCTGCA CCTATTCCCC CAAAAGATCC AAAACCAGAG 
CACGGGTCCC GGAAGAGGAC AGTCAGCCAA TCGTCTTCCT TAAAATCTAG CAGCAACAGT 
ATCAAGGAGA GCAGCAGCAG TAAAAACAGT TCTTCCTCTT CAAAGCAAAA AAAGACAGAA 
GGAAAGGTTT CTACCAGTTC CAAGGAAACC AAGGAAAAAA ATCCAAACAA CTCTTCAAAT 
TGTCCTCCAT CTACAACAAC TCCTGACAGT TCCAAGTCCC GAAGAGCAAA GCTTGCTTTT 
GATGACAGGA GCTATTCAGC AGATCATTAC TTACAAGAAG CAAAAAAACT AAAGCACAAT 
GCAGATGCGT TGTCCGATAG GTTTGAGAAA GCGGTCTTCT ACCTTGATGC TGTTGTGTCT 
TTCATTGAAT GTGGGAATGC ATTGGAGAAA AACGCTCAGG AATCCAAATC CCCGTTCCCT 
ATGTATTCAG AAACTGTGGA ACTAATTAAA TACACTATGA AGCTGAAGAA TTACTTGGCA 
CCAGATGCCA CAGCTGCAGA TAAAAGACTA GCCGTGCTAT GTCTGCGATG CCAGTCCCTG 
TTGTACCTGA GGTTGTTCAA GCTGAAGAAA GAGAGTGCGT TGAAATACTC AAAAACACTG 
ACGGAACATC TAAAGAACTC CTATAATAAT TCGCAAGCTC CCTCACCTGG CATAGGAAGC 
AAAGCCGCCG GGATGCCCTC GCCTGTCTCC CCCAAGCTTT CTCCGGGCAA TTCAGGAAAC 
TACTCCTCAG GGGCCAGCAA CCCTTCGGGG AGCGGTTCTT CGGTGACAAT TCCGCAGAGG 
ATCCACCAGA TGGCGGCCAG CTACGTCCAG GTCACCTCCA ACTTCCTCTA CGCGACTGAA 
ATTTGGGACC AAGCCGAACA GCTTTCCAAA GAGCAAAAAG AATTCTTTGC TGAACTGGAT 
AAAGTTATGG GCCCGCTCAT CTTTAATTCA AGCATCATGA CAGACCTAGT TCGTTACACC 
CGGCAGGGAC TCCACTGGTT GCGTCAGGAT GCCAAGTCGA TACCTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

AFF4 ( XM_029051767.1 ) cDNA ORF clone, Ornithorhynchus anatinus(platypus) -> XP_028907600.1 Ornithorhynchus anatinus AF4/FMR2 family member 4 (AFF4), transcript variant X1, mRNA.

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CloneID	OOc136283
Clone ID Related Accession (Same CDS sequence)	XM_029051767.1 , XM_029051768.1 , XM_029051769.1
Accession Version	XM_029051767.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3528bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1556380800000
Organism	Ornithorhynchus anatinus(platypus)
Product	AF4/FMR2 family member 4 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_041749.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Ornithorhynchus anatinus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAACCGTG AAGATCGGAA TGTGCTGCGT ATGAAAGAAA GGGAAAGGCG AAATCAGGAA 
ATTCAGCAGG GGGAAGAAGC CTTTCCACCT AACTCACCTC TCTTTGCTGA ACCTTATAAA 
GTTACCAGCA AGGAAGACAA ATTATCTAGT CGTATTCAGA GCATGCTCGG AAATTACGAT 
GAACAGGGCG TGCTTGGAAA CTACGATGAA ATGAAGGATT TTCGAGGAGA CCGATCTCTA 
CAGAAGCTGG TCGTGATCCC CAAGTCAACA GCGCCATCAG CAACAGATGA AAAATCCAAC 
CCAAGTTTCT TTGGTGAACA GAGACACAGT AGCTCTCATC AGAGCAGCAA ATGGACTCCT 
GTAGGGCCAG CACCTAGCAC TTCTTCCCAG TCCCAGAAAC GGTCCTCCGG TTTACAGAGT 
GGACACAGTA GCCAACGGAC CGGGGGCGGG GGCGGCGGCG GCGGCCAGAG GCATGACCGT 
GACTCTTACG GCGGCGGCGG CCGCAAAAAA ACTCAGCACG GAGCGGAACA CTCCAAATCG 
CGTTCTTCCA GCCCTGGGAA ACCCCCTGCT GTGTCTTCAT TAAGCTCCAG CCATTCCAGG 
TCTCATGGGA ATGATCATCA CAGTAAGGAA CATCAGCGCG CCAAGTCTCC TCGGGACCCC 
GAGGCAAACT GGGACTCGCC CTCCCGGGTT CCTTCATTTT CAAGCGGGCA GCACTCTAAC 
CAATCTTTCC CTCCTTCGTT GATGTCAAAG TCCAGCTCAA TGTTGCAGAA GCCCACTGCT 
TACGTAAGGC CCATGGATGG ACAGGAGTCC ATGGAACCAA AGCTATCCTC TGAGCACTAC 
AGCAGCCAAT CTCATAGCAA CAGCATGAGT GAGCTGAAGC CTAGCAGCAA AGCACACCTA 
ACCAAACTGA AAATTCCCTC TCAACCATTA GATGCATCAG TATCTGGTGA TGTGAGCTGT 
GTGGATGAAA TTCTCAAAGA GATGACTCAC TCATGGCCAC CCCCTCTAAC AGCCATTCAT 
ACGCCATGCA AAACAGAGCC TTCCAAATTT CCTTTTCCAA CCAAGGAGTC TCAGCAATCC 
AATTTTGGTA CTGGAGAACA AAAAAGATAC AATCCTCCAT CTAAAACATC AAATGGTCAC 
CAGGCCAAAT CATGTGAATC AAACCAGACA GATATGTTAA AAGATGACTT AAAACTCAGC 
AGCAGTGAAG ACAGTGATGG GGAACAGGAT TGTGATAAAA CAGTGCCAAG GAGTACCCCT 
GGAAGTAATT CTGAACCGTC ACACCATAAT AGTGAAGGAG CAGATAATTC CAGGGATGAC 
TCGAGCAGCC ATAGTGGATC CGAAAGCAGT TCCGGATCTG ACTCGGAAAG CGAGAGTAGT 
TCCAGTGACA GCGAGGCTAA CGAGCCATCC CAGAGTGCGT CTCCCGAGCC CGAGCCACCC 
CCAACCAACA AATGGCAACT GGACAATTGG TTGAATAAGG TGAATCCACA CAAAGTGTCA 
CCTGCTTCCT CTGTGGACAG CAACATCCCC TCCTCACAAG GTTATAAGAA GGAGGGTCGG 
GAGCAATGCC CTGGGAGTGG CTACGTCGAT CAGACAGGGC CTAAGGATTC CAACTCCTCC 
ACTCCCGGCC GAGATTCCAA AACCGTCCAA AAAGGATCCG AGGGTAGTCG CGGCAGACAA 
AAGTCTCCCG CCCAGAGTGA TAACACGACC CAAAGAAGAA CTGTGGGGAA GAAGCAGCCC 
AAAAAGGCAG AGAAAGCAGC TGTTGAGGAA CCTAGGGGAG GACTAAAAAT AGAAAGTGAA 
ACCCCCCTGG ATATGGCCAC AAGCATGCCC TCAAACAGGC ACAAGGCAGC CACAAAAGGC 
TCCAGGAAGC CCAACATAAA GAAGGAACCC AAGTCATCCC CTAGGCCCAC GACGGAGAAA 
AAGAAATACA AGTCAACAAG CAAATCTTCC CAGAAATCTC GGGAAATCAT AGAAACAGAT 
ACCTCATCCT CTGATTCTGA TGAAAGCGAG AGCCTCCCGC CTTCATCACA AACTCCTAAA 
TATCCCGAGA GCAATAGGAC TCCTGTTAAA CCCTCCTCCA TGGAGGAGGA AGACAGCTTT 
TTTCGGCAGA GAATGTTCTC TCCTATGGAA GAGAAGGAAC TCCTTTCACC ACTCAGTGAG 
CCTGATGACA GGTATCCTCT CATTGTGAAG ATTGACCTGA ATCTCTTGTC CAGAATACCA 
GGGAAGCCTT ACAAAGAAAC TGAGCCACCT AAGGTGGAAA AGAAAACTGT GCCAGAAAAG 
CACCCGAGAG ACCCACAGAA ACAAGCCTCA GACAAAGTCT CTAACAAGGG CAAGAGGAAA 
CATAAGAATG ATGATGACAA TAGACCCAGT GAAAGCAAGA AAACTAAACT GGAGGAAAAG 
GGTTCACTGA GCCACAAGAC ATCCAGCAAT AGAGAGTCAT CCAAACCAAG TGCTATAAAA 
GAGAAGGATC TTTTGCCTTC CCCAGCTGCA CCTATTCCCC CAAAAGATCC AAAACCAGAG 
CACGGGTCCC GGAAGAGGAC AGTCAGCCAA TCGTCTTCCT TAAAATCTAG CAGCAACAGT 
ATCAAGGAGA GCAGCAGCAG TAAAAACAGT TCTTCCTCTT CAAAGCAAAA AAAGACAGAA 
GGAAAGGTTT CTACCAGTTC CAAGGAAACC AAGGAAAAAA ATCCAAACAA CTCTTCAAAT 
TGTCCTCCAT CTACAACAAC TCCTGACAGT TCCAAGTCCC GAAGAGCAAA GCTTGCTTTT 
GATGACAGGA GCTATTCAGC AGATCATTAC TTACAAGAAG CAAAAAAACT AAAGCACAAT 
GCAGATGCGT TGTCCGATAG GTTTGAGAAA GCGGTCTTCT ACCTTGATGC TGTTGTGTCT 
TTCATTGAAT GTGGGAATGC ATTGGAGAAA AACGCTCAGG AATCCAAATC CCCGTTCCCT 
ATGTATTCAG AAACTGTGGA ACTAATTAAA TACACTATGA AGCTGAAGAA TTACTTGGCA 
CCAGATGCCA CAGCTGCAGA TAAAAGACTA GCCGTGCTAT GTCTGCGATG CCAGTCCCTG 
TTGTACCTGA GGTTGTTCAA GCTGAAGAAA GAGAGTGCGT TGAAATACTC AAAAACACTG 
ACGGAACATC TAAAGAACTC CTATAATAAT TCGCAAGCTC CCTCACCTGG CATAGGAAGC 
AAAGCCGCCG GGATGCCCTC GCCTGTCTCC CCCAAGCTTT CTCCGGGCAA TTCAGGAAAC 
TACTCCTCAG GGGCCAGCAA CCCTTCGGGG AGCGGTTCTT CGGTGACAAT TCCGCAGAGG 
ATCCACCAGA TGGCGGCCAG CTACGTCCAG GTCACCTCCA ACTTCCTCTA CGCGACTGAA 
ATTTGGGACC AAGCCGAACA GCTTTCCAAA GAGCAAAAAG AATTCTTTGC TGAACTGGAT 
AAAGTTATGG GCCCGCTCAT CTTTAATTCA AGCATCATGA CAGACCTAGT TCGTTACACC 
CGGCAGGGAC TCCACTGGTT GCGTCAGGAT GCCAAGTCGA TACCTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_028907600.1
CDS	441..3968
Translation	MNREDRNVLRMKERERRNQEIQQGEEAFPPNSPLFAEPYKVTSKEDKLSSRIQSMLGNYDEQGVLGNYDEMKDFRGDRSLQKLVVIPKSTAPSATDEKSNPSFFGEQRHSSSHQSSKWTPVGPAPSTSSQSQKRSSGLQSGHSSQRTGGGGGGGGQRHDRDSYGGGGRKKTQHGAEHSKSRSSSPGKPPAVSSLSSSHSRSHGNDHHSKEHQRAKSPRDPEANWDSPSRVPSFSSGQHSNQSFPPSLMSKSSSMLQKPTAYVRPMDGQESMEPKLSSEHYSSQSHSNSMSELKPSSKAHLTKLKIPSQPLDASVSGDVSCVDEILKEMTHSWPPPLTAIHTPCKTEPSKFPFPTKESQQSNFGTGEQKRYNPPSKTSNGHQAKSCESNQTDMLKDDLKLSSSEDSDGEQDCDKTVPRSTPGSNSEPSHHNSEGADNSRDDSSSHSGSESSSGSDSESESSSSDSEANEPSQSASPEPEPPPTNKWQLDNWLNKVNPHKVSPASSVDSNIPSSQGYKKEGREQCPGSGYVDQTGPKDSNSSTPGRDSKTVQKGSEGSRGRQKSPAQSDNTTQRRTVGKKQPKKAEKAAVEEPRGGLKIESETPLDMATSMPSNRHKAATKGSRKPNIKKEPKSSPRPTTEKKKYKSTSKSSQKSREIIETDTSSSDSDESESLPPSSQTPKYPESNRTPVKPSSMEEEDSFFRQRMFSPMEEKELLSPLSEPDDRYPLIVKIDLNLLSRIPGKPYKETEPPKVEKKTVPEKHPRDPQKQASDKVSNKGKRKHKNDDDNRPSESKKTKLEEKGSLSHKTSSNRESSKPSAIKEKDLLPSPAAPIPPKDPKPEHGSRKRTVSQSSSLKSSSNSIKESSSSKNSSSSSKQKKTEGKVSTSSKETKEKNPNNSSNCPPSTTTPDSSKSRRAKLAFDDRSYSADHYLQEAKKLKHNADALSDRFEKAVFYLDAVVSFIECGNALEKNAQESKSPFPMYSETVELIKYTMKLKNYLAPDATAADKRLAVLCLRCQSLLYLRLFKLKKESALKYSKTLTEHLKNSYNNSQAPSPGIGSKAAGMPSPVSPKLSPGNSGNYSSGASNPSGSGSSVTIPQRIHQMAASYVQVTSNFLYATEIWDQAEQLSKEQKEFFAELDKVMGPLIFNSSIMTDLVRYTRQGLHWLRQDAKSIP

Target ORF information:

RefSeq Version	XM_029051767.1
Organism	Ornithorhynchus anatinus(platypus)
Definition	Ornithorhynchus anatinus AF4/FMR2 family member 4 (AFF4), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_029051767.1

ORF Insert Sequence:

ATGAACCGTG AAGATCGGAA TGTGCTGCGT ATGAAAGAAA GGGAAAGGCG AAATCAGGAA 
ATTCAGCAGG GGGAAGAAGC CTTTCCACCT AACTCACCTC TCTTTGCTGA ACCTTATAAA 
GTTACCAGCA AGGAAGACAA ATTATCTAGT CGTATTCAGA GCATGCTCGG AAATTACGAT 
GAACAGGGCG TGCTTGGAAA CTACGATGAA ATGAAGGATT TTCGAGGAGA CCGATCTCTA 
CAGAAGCTGG TCGTGATCCC CAAGTCAACA GCGCCATCAG CAACAGATGA AAAATCCAAC 
CCAAGTTTCT TTGGTGAACA GAGACACAGT AGCTCTCATC AGAGCAGCAA ATGGACTCCT 
GTAGGGCCAG CACCTAGCAC TTCTTCCCAG TCCCAGAAAC GGTCCTCCGG TTTACAGAGT 
GGACACAGTA GCCAACGGAC CGGGGGCGGG GGCGGCGGCG GCGGCCAGAG GCATGACCGT 
GACTCTTACG GCGGCGGCGG CCGCAAAAAA ACTCAGCACG GAGCGGAACA CTCCAAATCG 
CGTTCTTCCA GCCCTGGGAA ACCCCCTGCT GTGTCTTCAT TAAGCTCCAG CCATTCCAGG 
TCTCATGGGA ATGATCATCA CAGTAAGGAA CATCAGCGCG CCAAGTCTCC TCGGGACCCC 
GAGGCAAACT GGGACTCGCC CTCCCGGGTT CCTTCATTTT CAAGCGGGCA GCACTCTAAC 
CAATCTTTCC CTCCTTCGTT GATGTCAAAG TCCAGCTCAA TGTTGCAGAA GCCCACTGCT 
TACGTAAGGC CCATGGATGG ACAGGAGTCC ATGGAACCAA AGCTATCCTC TGAGCACTAC 
AGCAGCCAAT CTCATAGCAA CAGCATGAGT GAGCTGAAGC CTAGCAGCAA AGCACACCTA 
ACCAAACTGA AAATTCCCTC TCAACCATTA GATGCATCAG TATCTGGTGA TGTGAGCTGT 
GTGGATGAAA TTCTCAAAGA GATGACTCAC TCATGGCCAC CCCCTCTAAC AGCCATTCAT 
ACGCCATGCA AAACAGAGCC TTCCAAATTT CCTTTTCCAA CCAAGGAGTC TCAGCAATCC 
AATTTTGGTA CTGGAGAACA AAAAAGATAC AATCCTCCAT CTAAAACATC AAATGGTCAC 
CAGGCCAAAT CATGTGAATC AAACCAGACA GATATGTTAA AAGATGACTT AAAACTCAGC 
AGCAGTGAAG ACAGTGATGG GGAACAGGAT TGTGATAAAA CAGTGCCAAG GAGTACCCCT 
GGAAGTAATT CTGAACCGTC ACACCATAAT AGTGAAGGAG CAGATAATTC CAGGGATGAC 
TCGAGCAGCC ATAGTGGATC CGAAAGCAGT TCCGGATCTG ACTCGGAAAG CGAGAGTAGT 
TCCAGTGACA GCGAGGCTAA CGAGCCATCC CAGAGTGCGT CTCCCGAGCC CGAGCCACCC 
CCAACCAACA AATGGCAACT GGACAATTGG TTGAATAAGG TGAATCCACA CAAAGTGTCA 
CCTGCTTCCT CTGTGGACAG CAACATCCCC TCCTCACAAG GTTATAAGAA GGAGGGTCGG 
GAGCAATGCC CTGGGAGTGG CTACGTCGAT CAGACAGGGC CTAAGGATTC CAACTCCTCC 
ACTCCCGGCC GAGATTCCAA AACCGTCCAA AAAGGATCCG AGGGTAGTCG CGGCAGACAA 
AAGTCTCCCG CCCAGAGTGA TAACACGACC CAAAGAAGAA CTGTGGGGAA GAAGCAGCCC 
AAAAAGGCAG AGAAAGCAGC TGTTGAGGAA CCTAGGGGAG GACTAAAAAT AGAAAGTGAA 
ACCCCCCTGG ATATGGCCAC AAGCATGCCC TCAAACAGGC ACAAGGCAGC CACAAAAGGC 
TCCAGGAAGC CCAACATAAA GAAGGAACCC AAGTCATCCC CTAGGCCCAC GACGGAGAAA 
AAGAAATACA AGTCAACAAG CAAATCTTCC CAGAAATCTC GGGAAATCAT AGAAACAGAT 
ACCTCATCCT CTGATTCTGA TGAAAGCGAG AGCCTCCCGC CTTCATCACA AACTCCTAAA 
TATCCCGAGA GCAATAGGAC TCCTGTTAAA CCCTCCTCCA TGGAGGAGGA AGACAGCTTT 
TTTCGGCAGA GAATGTTCTC TCCTATGGAA GAGAAGGAAC TCCTTTCACC ACTCAGTGAG 
CCTGATGACA GGTATCCTCT CATTGTGAAG ATTGACCTGA ATCTCTTGTC CAGAATACCA 
GGGAAGCCTT ACAAAGAAAC TGAGCCACCT AAGGTGGAAA AGAAAACTGT GCCAGAAAAG 
CACCCGAGAG ACCCACAGAA ACAAGCCTCA GACAAAGTCT CTAACAAGGG CAAGAGGAAA 
CATAAGAATG ATGATGACAA TAGACCCAGT GAAAGCAAGA AAACTAAACT GGAGGAAAAG 
GGTTCACTGA GCCACAAGAC ATCCAGCAAT AGAGAGTCAT CCAAACCAAG TGCTATAAAA 
GAGAAGGATC TTTTGCCTTC CCCAGCTGCA CCTATTCCCC CAAAAGATCC AAAACCAGAG 
CACGGGTCCC GGAAGAGGAC AGTCAGCCAA TCGTCTTCCT TAAAATCTAG CAGCAACAGT 
ATCAAGGAGA GCAGCAGCAG TAAAAACAGT TCTTCCTCTT CAAAGCAAAA AAAGACAGAA 
GGAAAGGTTT CTACCAGTTC CAAGGAAACC AAGGAAAAAA ATCCAAACAA CTCTTCAAAT 
TGTCCTCCAT CTACAACAAC TCCTGACAGT TCCAAGTCCC GAAGAGCAAA GCTTGCTTTT 
GATGACAGGA GCTATTCAGC AGATCATTAC TTACAAGAAG CAAAAAAACT AAAGCACAAT 
GCAGATGCGT TGTCCGATAG GTTTGAGAAA GCGGTCTTCT ACCTTGATGC TGTTGTGTCT 
TTCATTGAAT GTGGGAATGC ATTGGAGAAA AACGCTCAGG AATCCAAATC CCCGTTCCCT 
ATGTATTCAG AAACTGTGGA ACTAATTAAA TACACTATGA AGCTGAAGAA TTACTTGGCA 
CCAGATGCCA CAGCTGCAGA TAAAAGACTA GCCGTGCTAT GTCTGCGATG CCAGTCCCTG 
TTGTACCTGA GGTTGTTCAA GCTGAAGAAA GAGAGTGCGT TGAAATACTC AAAAACACTG 
ACGGAACATC TAAAGAACTC CTATAATAAT TCGCAAGCTC CCTCACCTGG CATAGGAAGC 
AAAGCCGCCG GGATGCCCTC GCCTGTCTCC CCCAAGCTTT CTCCGGGCAA TTCAGGAAAC 
TACTCCTCAG GGGCCAGCAA CCCTTCGGGG AGCGGTTCTT CGGTGACAAT TCCGCAGAGG 
ATCCACCAGA TGGCGGCCAG CTACGTCCAG GTCACCTCCA ACTTCCTCTA CGCGACTGAA 
ATTTGGGACC AAGCCGAACA GCTTTCCAAA GAGCAAAAAG AATTCTTTGC TGAACTGGAT 
AAAGTTATGG GCCCGCTCAT CTTTAATTCA AGCATCATGA CAGACCTAGT TCGTTACACC 
CGGCAGGGAC TCCACTGGTT GCGTCAGGAT GCCAAGTCGA TACCTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

AFF4 ( XM_029051768.1 ) cDNA ORF clone, Ornithorhynchus anatinus(platypus) -> XP_028907601.1 Ornithorhynchus anatinus AF4/FMR2 family member 4 (AFF4), transcript variant X2, mRNA.

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CloneID	OOc136283
Clone ID Related Accession (Same CDS sequence)	XM_029051767.1 , XM_029051768.1 , XM_029051769.1
Accession Version	XM_029051768.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3528bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1556380800000
Organism	Ornithorhynchus anatinus(platypus)
Product	AF4/FMR2 family member 4 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_041749.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Ornithorhynchus anatinus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAACCGTG AAGATCGGAA TGTGCTGCGT ATGAAAGAAA GGGAAAGGCG AAATCAGGAA 
ATTCAGCAGG GGGAAGAAGC CTTTCCACCT AACTCACCTC TCTTTGCTGA ACCTTATAAA 
GTTACCAGCA AGGAAGACAA ATTATCTAGT CGTATTCAGA GCATGCTCGG AAATTACGAT 
GAACAGGGCG TGCTTGGAAA CTACGATGAA ATGAAGGATT TTCGAGGAGA CCGATCTCTA 
CAGAAGCTGG TCGTGATCCC CAAGTCAACA GCGCCATCAG CAACAGATGA AAAATCCAAC 
CCAAGTTTCT TTGGTGAACA GAGACACAGT AGCTCTCATC AGAGCAGCAA ATGGACTCCT 
GTAGGGCCAG CACCTAGCAC TTCTTCCCAG TCCCAGAAAC GGTCCTCCGG TTTACAGAGT 
GGACACAGTA GCCAACGGAC CGGGGGCGGG GGCGGCGGCG GCGGCCAGAG GCATGACCGT 
GACTCTTACG GCGGCGGCGG CCGCAAAAAA ACTCAGCACG GAGCGGAACA CTCCAAATCG 
CGTTCTTCCA GCCCTGGGAA ACCCCCTGCT GTGTCTTCAT TAAGCTCCAG CCATTCCAGG 
TCTCATGGGA ATGATCATCA CAGTAAGGAA CATCAGCGCG CCAAGTCTCC TCGGGACCCC 
GAGGCAAACT GGGACTCGCC CTCCCGGGTT CCTTCATTTT CAAGCGGGCA GCACTCTAAC 
CAATCTTTCC CTCCTTCGTT GATGTCAAAG TCCAGCTCAA TGTTGCAGAA GCCCACTGCT 
TACGTAAGGC CCATGGATGG ACAGGAGTCC ATGGAACCAA AGCTATCCTC TGAGCACTAC 
AGCAGCCAAT CTCATAGCAA CAGCATGAGT GAGCTGAAGC CTAGCAGCAA AGCACACCTA 
ACCAAACTGA AAATTCCCTC TCAACCATTA GATGCATCAG TATCTGGTGA TGTGAGCTGT 
GTGGATGAAA TTCTCAAAGA GATGACTCAC TCATGGCCAC CCCCTCTAAC AGCCATTCAT 
ACGCCATGCA AAACAGAGCC TTCCAAATTT CCTTTTCCAA CCAAGGAGTC TCAGCAATCC 
AATTTTGGTA CTGGAGAACA AAAAAGATAC AATCCTCCAT CTAAAACATC AAATGGTCAC 
CAGGCCAAAT CATGTGAATC AAACCAGACA GATATGTTAA AAGATGACTT AAAACTCAGC 
AGCAGTGAAG ACAGTGATGG GGAACAGGAT TGTGATAAAA CAGTGCCAAG GAGTACCCCT 
GGAAGTAATT CTGAACCGTC ACACCATAAT AGTGAAGGAG CAGATAATTC CAGGGATGAC 
TCGAGCAGCC ATAGTGGATC CGAAAGCAGT TCCGGATCTG ACTCGGAAAG CGAGAGTAGT 
TCCAGTGACA GCGAGGCTAA CGAGCCATCC CAGAGTGCGT CTCCCGAGCC CGAGCCACCC 
CCAACCAACA AATGGCAACT GGACAATTGG TTGAATAAGG TGAATCCACA CAAAGTGTCA 
CCTGCTTCCT CTGTGGACAG CAACATCCCC TCCTCACAAG GTTATAAGAA GGAGGGTCGG 
GAGCAATGCC CTGGGAGTGG CTACGTCGAT CAGACAGGGC CTAAGGATTC CAACTCCTCC 
ACTCCCGGCC GAGATTCCAA AACCGTCCAA AAAGGATCCG AGGGTAGTCG CGGCAGACAA 
AAGTCTCCCG CCCAGAGTGA TAACACGACC CAAAGAAGAA CTGTGGGGAA GAAGCAGCCC 
AAAAAGGCAG AGAAAGCAGC TGTTGAGGAA CCTAGGGGAG GACTAAAAAT AGAAAGTGAA 
ACCCCCCTGG ATATGGCCAC AAGCATGCCC TCAAACAGGC ACAAGGCAGC CACAAAAGGC 
TCCAGGAAGC CCAACATAAA GAAGGAACCC AAGTCATCCC CTAGGCCCAC GACGGAGAAA 
AAGAAATACA AGTCAACAAG CAAATCTTCC CAGAAATCTC GGGAAATCAT AGAAACAGAT 
ACCTCATCCT CTGATTCTGA TGAAAGCGAG AGCCTCCCGC CTTCATCACA AACTCCTAAA 
TATCCCGAGA GCAATAGGAC TCCTGTTAAA CCCTCCTCCA TGGAGGAGGA AGACAGCTTT 
TTTCGGCAGA GAATGTTCTC TCCTATGGAA GAGAAGGAAC TCCTTTCACC ACTCAGTGAG 
CCTGATGACA GGTATCCTCT CATTGTGAAG ATTGACCTGA ATCTCTTGTC CAGAATACCA 
GGGAAGCCTT ACAAAGAAAC TGAGCCACCT AAGGTGGAAA AGAAAACTGT GCCAGAAAAG 
CACCCGAGAG ACCCACAGAA ACAAGCCTCA GACAAAGTCT CTAACAAGGG CAAGAGGAAA 
CATAAGAATG ATGATGACAA TAGACCCAGT GAAAGCAAGA AAACTAAACT GGAGGAAAAG 
GGTTCACTGA GCCACAAGAC ATCCAGCAAT AGAGAGTCAT CCAAACCAAG TGCTATAAAA 
GAGAAGGATC TTTTGCCTTC CCCAGCTGCA CCTATTCCCC CAAAAGATCC AAAACCAGAG 
CACGGGTCCC GGAAGAGGAC AGTCAGCCAA TCGTCTTCCT TAAAATCTAG CAGCAACAGT 
ATCAAGGAGA GCAGCAGCAG TAAAAACAGT TCTTCCTCTT CAAAGCAAAA AAAGACAGAA 
GGAAAGGTTT CTACCAGTTC CAAGGAAACC AAGGAAAAAA ATCCAAACAA CTCTTCAAAT 
TGTCCTCCAT CTACAACAAC TCCTGACAGT TCCAAGTCCC GAAGAGCAAA GCTTGCTTTT 
GATGACAGGA GCTATTCAGC AGATCATTAC TTACAAGAAG CAAAAAAACT AAAGCACAAT 
GCAGATGCGT TGTCCGATAG GTTTGAGAAA GCGGTCTTCT ACCTTGATGC TGTTGTGTCT 
TTCATTGAAT GTGGGAATGC ATTGGAGAAA AACGCTCAGG AATCCAAATC CCCGTTCCCT 
ATGTATTCAG AAACTGTGGA ACTAATTAAA TACACTATGA AGCTGAAGAA TTACTTGGCA 
CCAGATGCCA CAGCTGCAGA TAAAAGACTA GCCGTGCTAT GTCTGCGATG CCAGTCCCTG 
TTGTACCTGA GGTTGTTCAA GCTGAAGAAA GAGAGTGCGT TGAAATACTC AAAAACACTG 
ACGGAACATC TAAAGAACTC CTATAATAAT TCGCAAGCTC CCTCACCTGG CATAGGAAGC 
AAAGCCGCCG GGATGCCCTC GCCTGTCTCC CCCAAGCTTT CTCCGGGCAA TTCAGGAAAC 
TACTCCTCAG GGGCCAGCAA CCCTTCGGGG AGCGGTTCTT CGGTGACAAT TCCGCAGAGG 
ATCCACCAGA TGGCGGCCAG CTACGTCCAG GTCACCTCCA ACTTCCTCTA CGCGACTGAA 
ATTTGGGACC AAGCCGAACA GCTTTCCAAA GAGCAAAAAG AATTCTTTGC TGAACTGGAT 
AAAGTTATGG GCCCGCTCAT CTTTAATTCA AGCATCATGA CAGACCTAGT TCGTTACACC 
CGGCAGGGAC TCCACTGGTT GCGTCAGGAT GCCAAGTCGA TACCTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_028907601.1
CDS	348..3875
Translation	MNREDRNVLRMKERERRNQEIQQGEEAFPPNSPLFAEPYKVTSKEDKLSSRIQSMLGNYDEQGVLGNYDEMKDFRGDRSLQKLVVIPKSTAPSATDEKSNPSFFGEQRHSSSHQSSKWTPVGPAPSTSSQSQKRSSGLQSGHSSQRTGGGGGGGGQRHDRDSYGGGGRKKTQHGAEHSKSRSSSPGKPPAVSSLSSSHSRSHGNDHHSKEHQRAKSPRDPEANWDSPSRVPSFSSGQHSNQSFPPSLMSKSSSMLQKPTAYVRPMDGQESMEPKLSSEHYSSQSHSNSMSELKPSSKAHLTKLKIPSQPLDASVSGDVSCVDEILKEMTHSWPPPLTAIHTPCKTEPSKFPFPTKESQQSNFGTGEQKRYNPPSKTSNGHQAKSCESNQTDMLKDDLKLSSSEDSDGEQDCDKTVPRSTPGSNSEPSHHNSEGADNSRDDSSSHSGSESSSGSDSESESSSSDSEANEPSQSASPEPEPPPTNKWQLDNWLNKVNPHKVSPASSVDSNIPSSQGYKKEGREQCPGSGYVDQTGPKDSNSSTPGRDSKTVQKGSEGSRGRQKSPAQSDNTTQRRTVGKKQPKKAEKAAVEEPRGGLKIESETPLDMATSMPSNRHKAATKGSRKPNIKKEPKSSPRPTTEKKKYKSTSKSSQKSREIIETDTSSSDSDESESLPPSSQTPKYPESNRTPVKPSSMEEEDSFFRQRMFSPMEEKELLSPLSEPDDRYPLIVKIDLNLLSRIPGKPYKETEPPKVEKKTVPEKHPRDPQKQASDKVSNKGKRKHKNDDDNRPSESKKTKLEEKGSLSHKTSSNRESSKPSAIKEKDLLPSPAAPIPPKDPKPEHGSRKRTVSQSSSLKSSSNSIKESSSSKNSSSSSKQKKTEGKVSTSSKETKEKNPNNSSNCPPSTTTPDSSKSRRAKLAFDDRSYSADHYLQEAKKLKHNADALSDRFEKAVFYLDAVVSFIECGNALEKNAQESKSPFPMYSETVELIKYTMKLKNYLAPDATAADKRLAVLCLRCQSLLYLRLFKLKKESALKYSKTLTEHLKNSYNNSQAPSPGIGSKAAGMPSPVSPKLSPGNSGNYSSGASNPSGSGSSVTIPQRIHQMAASYVQVTSNFLYATEIWDQAEQLSKEQKEFFAELDKVMGPLIFNSSIMTDLVRYTRQGLHWLRQDAKSIP

Target ORF information:

RefSeq Version	XM_029051768.1
Organism	Ornithorhynchus anatinus(platypus)
Definition	Ornithorhynchus anatinus AF4/FMR2 family member 4 (AFF4), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_029051768.1

ORF Insert Sequence:

ATGAACCGTG AAGATCGGAA TGTGCTGCGT ATGAAAGAAA GGGAAAGGCG AAATCAGGAA 
ATTCAGCAGG GGGAAGAAGC CTTTCCACCT AACTCACCTC TCTTTGCTGA ACCTTATAAA 
GTTACCAGCA AGGAAGACAA ATTATCTAGT CGTATTCAGA GCATGCTCGG AAATTACGAT 
GAACAGGGCG TGCTTGGAAA CTACGATGAA ATGAAGGATT TTCGAGGAGA CCGATCTCTA 
CAGAAGCTGG TCGTGATCCC CAAGTCAACA GCGCCATCAG CAACAGATGA AAAATCCAAC 
CCAAGTTTCT TTGGTGAACA GAGACACAGT AGCTCTCATC AGAGCAGCAA ATGGACTCCT 
GTAGGGCCAG CACCTAGCAC TTCTTCCCAG TCCCAGAAAC GGTCCTCCGG TTTACAGAGT 
GGACACAGTA GCCAACGGAC CGGGGGCGGG GGCGGCGGCG GCGGCCAGAG GCATGACCGT 
GACTCTTACG GCGGCGGCGG CCGCAAAAAA ACTCAGCACG GAGCGGAACA CTCCAAATCG 
CGTTCTTCCA GCCCTGGGAA ACCCCCTGCT GTGTCTTCAT TAAGCTCCAG CCATTCCAGG 
TCTCATGGGA ATGATCATCA CAGTAAGGAA CATCAGCGCG CCAAGTCTCC TCGGGACCCC 
GAGGCAAACT GGGACTCGCC CTCCCGGGTT CCTTCATTTT CAAGCGGGCA GCACTCTAAC 
CAATCTTTCC CTCCTTCGTT GATGTCAAAG TCCAGCTCAA TGTTGCAGAA GCCCACTGCT 
TACGTAAGGC CCATGGATGG ACAGGAGTCC ATGGAACCAA AGCTATCCTC TGAGCACTAC 
AGCAGCCAAT CTCATAGCAA CAGCATGAGT GAGCTGAAGC CTAGCAGCAA AGCACACCTA 
ACCAAACTGA AAATTCCCTC TCAACCATTA GATGCATCAG TATCTGGTGA TGTGAGCTGT 
GTGGATGAAA TTCTCAAAGA GATGACTCAC TCATGGCCAC CCCCTCTAAC AGCCATTCAT 
ACGCCATGCA AAACAGAGCC TTCCAAATTT CCTTTTCCAA CCAAGGAGTC TCAGCAATCC 
AATTTTGGTA CTGGAGAACA AAAAAGATAC AATCCTCCAT CTAAAACATC AAATGGTCAC 
CAGGCCAAAT CATGTGAATC AAACCAGACA GATATGTTAA AAGATGACTT AAAACTCAGC 
AGCAGTGAAG ACAGTGATGG GGAACAGGAT TGTGATAAAA CAGTGCCAAG GAGTACCCCT 
GGAAGTAATT CTGAACCGTC ACACCATAAT AGTGAAGGAG CAGATAATTC CAGGGATGAC 
TCGAGCAGCC ATAGTGGATC CGAAAGCAGT TCCGGATCTG ACTCGGAAAG CGAGAGTAGT 
TCCAGTGACA GCGAGGCTAA CGAGCCATCC CAGAGTGCGT CTCCCGAGCC CGAGCCACCC 
CCAACCAACA AATGGCAACT GGACAATTGG TTGAATAAGG TGAATCCACA CAAAGTGTCA 
CCTGCTTCCT CTGTGGACAG CAACATCCCC TCCTCACAAG GTTATAAGAA GGAGGGTCGG 
GAGCAATGCC CTGGGAGTGG CTACGTCGAT CAGACAGGGC CTAAGGATTC CAACTCCTCC 
ACTCCCGGCC GAGATTCCAA AACCGTCCAA AAAGGATCCG AGGGTAGTCG CGGCAGACAA 
AAGTCTCCCG CCCAGAGTGA TAACACGACC CAAAGAAGAA CTGTGGGGAA GAAGCAGCCC 
AAAAAGGCAG AGAAAGCAGC TGTTGAGGAA CCTAGGGGAG GACTAAAAAT AGAAAGTGAA 
ACCCCCCTGG ATATGGCCAC AAGCATGCCC TCAAACAGGC ACAAGGCAGC CACAAAAGGC 
TCCAGGAAGC CCAACATAAA GAAGGAACCC AAGTCATCCC CTAGGCCCAC GACGGAGAAA 
AAGAAATACA AGTCAACAAG CAAATCTTCC CAGAAATCTC GGGAAATCAT AGAAACAGAT 
ACCTCATCCT CTGATTCTGA TGAAAGCGAG AGCCTCCCGC CTTCATCACA AACTCCTAAA 
TATCCCGAGA GCAATAGGAC TCCTGTTAAA CCCTCCTCCA TGGAGGAGGA AGACAGCTTT 
TTTCGGCAGA GAATGTTCTC TCCTATGGAA GAGAAGGAAC TCCTTTCACC ACTCAGTGAG 
CCTGATGACA GGTATCCTCT CATTGTGAAG ATTGACCTGA ATCTCTTGTC CAGAATACCA 
GGGAAGCCTT ACAAAGAAAC TGAGCCACCT AAGGTGGAAA AGAAAACTGT GCCAGAAAAG 
CACCCGAGAG ACCCACAGAA ACAAGCCTCA GACAAAGTCT CTAACAAGGG CAAGAGGAAA 
CATAAGAATG ATGATGACAA TAGACCCAGT GAAAGCAAGA AAACTAAACT GGAGGAAAAG 
GGTTCACTGA GCCACAAGAC ATCCAGCAAT AGAGAGTCAT CCAAACCAAG TGCTATAAAA 
GAGAAGGATC TTTTGCCTTC CCCAGCTGCA CCTATTCCCC CAAAAGATCC AAAACCAGAG 
CACGGGTCCC GGAAGAGGAC AGTCAGCCAA TCGTCTTCCT TAAAATCTAG CAGCAACAGT 
ATCAAGGAGA GCAGCAGCAG TAAAAACAGT TCTTCCTCTT CAAAGCAAAA AAAGACAGAA 
GGAAAGGTTT CTACCAGTTC CAAGGAAACC AAGGAAAAAA ATCCAAACAA CTCTTCAAAT 
TGTCCTCCAT CTACAACAAC TCCTGACAGT TCCAAGTCCC GAAGAGCAAA GCTTGCTTTT 
GATGACAGGA GCTATTCAGC AGATCATTAC TTACAAGAAG CAAAAAAACT AAAGCACAAT 
GCAGATGCGT TGTCCGATAG GTTTGAGAAA GCGGTCTTCT ACCTTGATGC TGTTGTGTCT 
TTCATTGAAT GTGGGAATGC ATTGGAGAAA AACGCTCAGG AATCCAAATC CCCGTTCCCT 
ATGTATTCAG AAACTGTGGA ACTAATTAAA TACACTATGA AGCTGAAGAA TTACTTGGCA 
CCAGATGCCA CAGCTGCAGA TAAAAGACTA GCCGTGCTAT GTCTGCGATG CCAGTCCCTG 
TTGTACCTGA GGTTGTTCAA GCTGAAGAAA GAGAGTGCGT TGAAATACTC AAAAACACTG 
ACGGAACATC TAAAGAACTC CTATAATAAT TCGCAAGCTC CCTCACCTGG CATAGGAAGC 
AAAGCCGCCG GGATGCCCTC GCCTGTCTCC CCCAAGCTTT CTCCGGGCAA TTCAGGAAAC 
TACTCCTCAG GGGCCAGCAA CCCTTCGGGG AGCGGTTCTT CGGTGACAAT TCCGCAGAGG 
ATCCACCAGA TGGCGGCCAG CTACGTCCAG GTCACCTCCA ACTTCCTCTA CGCGACTGAA 
ATTTGGGACC AAGCCGAACA GCTTTCCAAA GAGCAAAAAG AATTCTTTGC TGAACTGGAT 
AAAGTTATGG GCCCGCTCAT CTTTAATTCA AGCATCATGA CAGACCTAGT TCGTTACACC 
CGGCAGGGAC TCCACTGGTT GCGTCAGGAT GCCAAGTCGA TACCTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

AFF4 ( XM_029051769.1 ) cDNA ORF clone, Ornithorhynchus anatinus(platypus) -> XP_028907602.1 Ornithorhynchus anatinus AF4/FMR2 family member 4 (AFF4), transcript variant X3, mRNA.

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CloneID	OOc136283
Clone ID Related Accession (Same CDS sequence)	XM_029051767.1 , XM_029051768.1 , XM_029051769.1
Accession Version	XM_029051769.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3528bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1556380800000
Organism	Ornithorhynchus anatinus(platypus)
Product	AF4/FMR2 family member 4 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_041749.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Ornithorhynchus anatinus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAACCGTG AAGATCGGAA TGTGCTGCGT ATGAAAGAAA GGGAAAGGCG AAATCAGGAA 
ATTCAGCAGG GGGAAGAAGC CTTTCCACCT AACTCACCTC TCTTTGCTGA ACCTTATAAA 
GTTACCAGCA AGGAAGACAA ATTATCTAGT CGTATTCAGA GCATGCTCGG AAATTACGAT 
GAACAGGGCG TGCTTGGAAA CTACGATGAA ATGAAGGATT TTCGAGGAGA CCGATCTCTA 
CAGAAGCTGG TCGTGATCCC CAAGTCAACA GCGCCATCAG CAACAGATGA AAAATCCAAC 
CCAAGTTTCT TTGGTGAACA GAGACACAGT AGCTCTCATC AGAGCAGCAA ATGGACTCCT 
GTAGGGCCAG CACCTAGCAC TTCTTCCCAG TCCCAGAAAC GGTCCTCCGG TTTACAGAGT 
GGACACAGTA GCCAACGGAC CGGGGGCGGG GGCGGCGGCG GCGGCCAGAG GCATGACCGT 
GACTCTTACG GCGGCGGCGG CCGCAAAAAA ACTCAGCACG GAGCGGAACA CTCCAAATCG 
CGTTCTTCCA GCCCTGGGAA ACCCCCTGCT GTGTCTTCAT TAAGCTCCAG CCATTCCAGG 
TCTCATGGGA ATGATCATCA CAGTAAGGAA CATCAGCGCG CCAAGTCTCC TCGGGACCCC 
GAGGCAAACT GGGACTCGCC CTCCCGGGTT CCTTCATTTT CAAGCGGGCA GCACTCTAAC 
CAATCTTTCC CTCCTTCGTT GATGTCAAAG TCCAGCTCAA TGTTGCAGAA GCCCACTGCT 
TACGTAAGGC CCATGGATGG ACAGGAGTCC ATGGAACCAA AGCTATCCTC TGAGCACTAC 
AGCAGCCAAT CTCATAGCAA CAGCATGAGT GAGCTGAAGC CTAGCAGCAA AGCACACCTA 
ACCAAACTGA AAATTCCCTC TCAACCATTA GATGCATCAG TATCTGGTGA TGTGAGCTGT 
GTGGATGAAA TTCTCAAAGA GATGACTCAC TCATGGCCAC CCCCTCTAAC AGCCATTCAT 
ACGCCATGCA AAACAGAGCC TTCCAAATTT CCTTTTCCAA CCAAGGAGTC TCAGCAATCC 
AATTTTGGTA CTGGAGAACA AAAAAGATAC AATCCTCCAT CTAAAACATC AAATGGTCAC 
CAGGCCAAAT CATGTGAATC AAACCAGACA GATATGTTAA AAGATGACTT AAAACTCAGC 
AGCAGTGAAG ACAGTGATGG GGAACAGGAT TGTGATAAAA CAGTGCCAAG GAGTACCCCT 
GGAAGTAATT CTGAACCGTC ACACCATAAT AGTGAAGGAG CAGATAATTC CAGGGATGAC 
TCGAGCAGCC ATAGTGGATC CGAAAGCAGT TCCGGATCTG ACTCGGAAAG CGAGAGTAGT 
TCCAGTGACA GCGAGGCTAA CGAGCCATCC CAGAGTGCGT CTCCCGAGCC CGAGCCACCC 
CCAACCAACA AATGGCAACT GGACAATTGG TTGAATAAGG TGAATCCACA CAAAGTGTCA 
CCTGCTTCCT CTGTGGACAG CAACATCCCC TCCTCACAAG GTTATAAGAA GGAGGGTCGG 
GAGCAATGCC CTGGGAGTGG CTACGTCGAT CAGACAGGGC CTAAGGATTC CAACTCCTCC 
ACTCCCGGCC GAGATTCCAA AACCGTCCAA AAAGGATCCG AGGGTAGTCG CGGCAGACAA 
AAGTCTCCCG CCCAGAGTGA TAACACGACC CAAAGAAGAA CTGTGGGGAA GAAGCAGCCC 
AAAAAGGCAG AGAAAGCAGC TGTTGAGGAA CCTAGGGGAG GACTAAAAAT AGAAAGTGAA 
ACCCCCCTGG ATATGGCCAC AAGCATGCCC TCAAACAGGC ACAAGGCAGC CACAAAAGGC 
TCCAGGAAGC CCAACATAAA GAAGGAACCC AAGTCATCCC CTAGGCCCAC GACGGAGAAA 
AAGAAATACA AGTCAACAAG CAAATCTTCC CAGAAATCTC GGGAAATCAT AGAAACAGAT 
ACCTCATCCT CTGATTCTGA TGAAAGCGAG AGCCTCCCGC CTTCATCACA AACTCCTAAA 
TATCCCGAGA GCAATAGGAC TCCTGTTAAA CCCTCCTCCA TGGAGGAGGA AGACAGCTTT 
TTTCGGCAGA GAATGTTCTC TCCTATGGAA GAGAAGGAAC TCCTTTCACC ACTCAGTGAG 
CCTGATGACA GGTATCCTCT CATTGTGAAG ATTGACCTGA ATCTCTTGTC CAGAATACCA 
GGGAAGCCTT ACAAAGAAAC TGAGCCACCT AAGGTGGAAA AGAAAACTGT GCCAGAAAAG 
CACCCGAGAG ACCCACAGAA ACAAGCCTCA GACAAAGTCT CTAACAAGGG CAAGAGGAAA 
CATAAGAATG ATGATGACAA TAGACCCAGT GAAAGCAAGA AAACTAAACT GGAGGAAAAG 
GGTTCACTGA GCCACAAGAC ATCCAGCAAT AGAGAGTCAT CCAAACCAAG TGCTATAAAA 
GAGAAGGATC TTTTGCCTTC CCCAGCTGCA CCTATTCCCC CAAAAGATCC AAAACCAGAG 
CACGGGTCCC GGAAGAGGAC AGTCAGCCAA TCGTCTTCCT TAAAATCTAG CAGCAACAGT 
ATCAAGGAGA GCAGCAGCAG TAAAAACAGT TCTTCCTCTT CAAAGCAAAA AAAGACAGAA 
GGAAAGGTTT CTACCAGTTC CAAGGAAACC AAGGAAAAAA ATCCAAACAA CTCTTCAAAT 
TGTCCTCCAT CTACAACAAC TCCTGACAGT TCCAAGTCCC GAAGAGCAAA GCTTGCTTTT 
GATGACAGGA GCTATTCAGC AGATCATTAC TTACAAGAAG CAAAAAAACT AAAGCACAAT 
GCAGATGCGT TGTCCGATAG GTTTGAGAAA GCGGTCTTCT ACCTTGATGC TGTTGTGTCT 
TTCATTGAAT GTGGGAATGC ATTGGAGAAA AACGCTCAGG AATCCAAATC CCCGTTCCCT 
ATGTATTCAG AAACTGTGGA ACTAATTAAA TACACTATGA AGCTGAAGAA TTACTTGGCA 
CCAGATGCCA CAGCTGCAGA TAAAAGACTA GCCGTGCTAT GTCTGCGATG CCAGTCCCTG 
TTGTACCTGA GGTTGTTCAA GCTGAAGAAA GAGAGTGCGT TGAAATACTC AAAAACACTG 
ACGGAACATC TAAAGAACTC CTATAATAAT TCGCAAGCTC CCTCACCTGG CATAGGAAGC 
AAAGCCGCCG GGATGCCCTC GCCTGTCTCC CCCAAGCTTT CTCCGGGCAA TTCAGGAAAC 
TACTCCTCAG GGGCCAGCAA CCCTTCGGGG AGCGGTTCTT CGGTGACAAT TCCGCAGAGG 
ATCCACCAGA TGGCGGCCAG CTACGTCCAG GTCACCTCCA ACTTCCTCTA CGCGACTGAA 
ATTTGGGACC AAGCCGAACA GCTTTCCAAA GAGCAAAAAG AATTCTTTGC TGAACTGGAT 
AAAGTTATGG GCCCGCTCAT CTTTAATTCA AGCATCATGA CAGACCTAGT TCGTTACACC 
CGGCAGGGAC TCCACTGGTT GCGTCAGGAT GCCAAGTCGA TACCTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_028907602.1
CDS	27..3554
Translation	MNREDRNVLRMKERERRNQEIQQGEEAFPPNSPLFAEPYKVTSKEDKLSSRIQSMLGNYDEQGVLGNYDEMKDFRGDRSLQKLVVIPKSTAPSATDEKSNPSFFGEQRHSSSHQSSKWTPVGPAPSTSSQSQKRSSGLQSGHSSQRTGGGGGGGGQRHDRDSYGGGGRKKTQHGAEHSKSRSSSPGKPPAVSSLSSSHSRSHGNDHHSKEHQRAKSPRDPEANWDSPSRVPSFSSGQHSNQSFPPSLMSKSSSMLQKPTAYVRPMDGQESMEPKLSSEHYSSQSHSNSMSELKPSSKAHLTKLKIPSQPLDASVSGDVSCVDEILKEMTHSWPPPLTAIHTPCKTEPSKFPFPTKESQQSNFGTGEQKRYNPPSKTSNGHQAKSCESNQTDMLKDDLKLSSSEDSDGEQDCDKTVPRSTPGSNSEPSHHNSEGADNSRDDSSSHSGSESSSGSDSESESSSSDSEANEPSQSASPEPEPPPTNKWQLDNWLNKVNPHKVSPASSVDSNIPSSQGYKKEGREQCPGSGYVDQTGPKDSNSSTPGRDSKTVQKGSEGSRGRQKSPAQSDNTTQRRTVGKKQPKKAEKAAVEEPRGGLKIESETPLDMATSMPSNRHKAATKGSRKPNIKKEPKSSPRPTTEKKKYKSTSKSSQKSREIIETDTSSSDSDESESLPPSSQTPKYPESNRTPVKPSSMEEEDSFFRQRMFSPMEEKELLSPLSEPDDRYPLIVKIDLNLLSRIPGKPYKETEPPKVEKKTVPEKHPRDPQKQASDKVSNKGKRKHKNDDDNRPSESKKTKLEEKGSLSHKTSSNRESSKPSAIKEKDLLPSPAAPIPPKDPKPEHGSRKRTVSQSSSLKSSSNSIKESSSSKNSSSSSKQKKTEGKVSTSSKETKEKNPNNSSNCPPSTTTPDSSKSRRAKLAFDDRSYSADHYLQEAKKLKHNADALSDRFEKAVFYLDAVVSFIECGNALEKNAQESKSPFPMYSETVELIKYTMKLKNYLAPDATAADKRLAVLCLRCQSLLYLRLFKLKKESALKYSKTLTEHLKNSYNNSQAPSPGIGSKAAGMPSPVSPKLSPGNSGNYSSGASNPSGSGSSVTIPQRIHQMAASYVQVTSNFLYATEIWDQAEQLSKEQKEFFAELDKVMGPLIFNSSIMTDLVRYTRQGLHWLRQDAKSIP

Target ORF information:

RefSeq Version	XM_029051769.1
Organism	Ornithorhynchus anatinus(platypus)
Definition	Ornithorhynchus anatinus AF4/FMR2 family member 4 (AFF4), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_029051769.1

ORF Insert Sequence:

ATGAACCGTG AAGATCGGAA TGTGCTGCGT ATGAAAGAAA GGGAAAGGCG AAATCAGGAA 
ATTCAGCAGG GGGAAGAAGC CTTTCCACCT AACTCACCTC TCTTTGCTGA ACCTTATAAA 
GTTACCAGCA AGGAAGACAA ATTATCTAGT CGTATTCAGA GCATGCTCGG AAATTACGAT 
GAACAGGGCG TGCTTGGAAA CTACGATGAA ATGAAGGATT TTCGAGGAGA CCGATCTCTA 
CAGAAGCTGG TCGTGATCCC CAAGTCAACA GCGCCATCAG CAACAGATGA AAAATCCAAC 
CCAAGTTTCT TTGGTGAACA GAGACACAGT AGCTCTCATC AGAGCAGCAA ATGGACTCCT 
GTAGGGCCAG CACCTAGCAC TTCTTCCCAG TCCCAGAAAC GGTCCTCCGG TTTACAGAGT 
GGACACAGTA GCCAACGGAC CGGGGGCGGG GGCGGCGGCG GCGGCCAGAG GCATGACCGT 
GACTCTTACG GCGGCGGCGG CCGCAAAAAA ACTCAGCACG GAGCGGAACA CTCCAAATCG 
CGTTCTTCCA GCCCTGGGAA ACCCCCTGCT GTGTCTTCAT TAAGCTCCAG CCATTCCAGG 
TCTCATGGGA ATGATCATCA CAGTAAGGAA CATCAGCGCG CCAAGTCTCC TCGGGACCCC 
GAGGCAAACT GGGACTCGCC CTCCCGGGTT CCTTCATTTT CAAGCGGGCA GCACTCTAAC 
CAATCTTTCC CTCCTTCGTT GATGTCAAAG TCCAGCTCAA TGTTGCAGAA GCCCACTGCT 
TACGTAAGGC CCATGGATGG ACAGGAGTCC ATGGAACCAA AGCTATCCTC TGAGCACTAC 
AGCAGCCAAT CTCATAGCAA CAGCATGAGT GAGCTGAAGC CTAGCAGCAA AGCACACCTA 
ACCAAACTGA AAATTCCCTC TCAACCATTA GATGCATCAG TATCTGGTGA TGTGAGCTGT 
GTGGATGAAA TTCTCAAAGA GATGACTCAC TCATGGCCAC CCCCTCTAAC AGCCATTCAT 
ACGCCATGCA AAACAGAGCC TTCCAAATTT CCTTTTCCAA CCAAGGAGTC TCAGCAATCC 
AATTTTGGTA CTGGAGAACA AAAAAGATAC AATCCTCCAT CTAAAACATC AAATGGTCAC 
CAGGCCAAAT CATGTGAATC AAACCAGACA GATATGTTAA AAGATGACTT AAAACTCAGC 
AGCAGTGAAG ACAGTGATGG GGAACAGGAT TGTGATAAAA CAGTGCCAAG GAGTACCCCT 
GGAAGTAATT CTGAACCGTC ACACCATAAT AGTGAAGGAG CAGATAATTC CAGGGATGAC 
TCGAGCAGCC ATAGTGGATC CGAAAGCAGT TCCGGATCTG ACTCGGAAAG CGAGAGTAGT 
TCCAGTGACA GCGAGGCTAA CGAGCCATCC CAGAGTGCGT CTCCCGAGCC CGAGCCACCC 
CCAACCAACA AATGGCAACT GGACAATTGG TTGAATAAGG TGAATCCACA CAAAGTGTCA 
CCTGCTTCCT CTGTGGACAG CAACATCCCC TCCTCACAAG GTTATAAGAA GGAGGGTCGG 
GAGCAATGCC CTGGGAGTGG CTACGTCGAT CAGACAGGGC CTAAGGATTC CAACTCCTCC 
ACTCCCGGCC GAGATTCCAA AACCGTCCAA AAAGGATCCG AGGGTAGTCG CGGCAGACAA 
AAGTCTCCCG CCCAGAGTGA TAACACGACC CAAAGAAGAA CTGTGGGGAA GAAGCAGCCC 
AAAAAGGCAG AGAAAGCAGC TGTTGAGGAA CCTAGGGGAG GACTAAAAAT AGAAAGTGAA 
ACCCCCCTGG ATATGGCCAC AAGCATGCCC TCAAACAGGC ACAAGGCAGC CACAAAAGGC 
TCCAGGAAGC CCAACATAAA GAAGGAACCC AAGTCATCCC CTAGGCCCAC GACGGAGAAA 
AAGAAATACA AGTCAACAAG CAAATCTTCC CAGAAATCTC GGGAAATCAT AGAAACAGAT 
ACCTCATCCT CTGATTCTGA TGAAAGCGAG AGCCTCCCGC CTTCATCACA AACTCCTAAA 
TATCCCGAGA GCAATAGGAC TCCTGTTAAA CCCTCCTCCA TGGAGGAGGA AGACAGCTTT 
TTTCGGCAGA GAATGTTCTC TCCTATGGAA GAGAAGGAAC TCCTTTCACC ACTCAGTGAG 
CCTGATGACA GGTATCCTCT CATTGTGAAG ATTGACCTGA ATCTCTTGTC CAGAATACCA 
GGGAAGCCTT ACAAAGAAAC TGAGCCACCT AAGGTGGAAA AGAAAACTGT GCCAGAAAAG 
CACCCGAGAG ACCCACAGAA ACAAGCCTCA GACAAAGTCT CTAACAAGGG CAAGAGGAAA 
CATAAGAATG ATGATGACAA TAGACCCAGT GAAAGCAAGA AAACTAAACT GGAGGAAAAG 
GGTTCACTGA GCCACAAGAC ATCCAGCAAT AGAGAGTCAT CCAAACCAAG TGCTATAAAA 
GAGAAGGATC TTTTGCCTTC CCCAGCTGCA CCTATTCCCC CAAAAGATCC AAAACCAGAG 
CACGGGTCCC GGAAGAGGAC AGTCAGCCAA TCGTCTTCCT TAAAATCTAG CAGCAACAGT 
ATCAAGGAGA GCAGCAGCAG TAAAAACAGT TCTTCCTCTT CAAAGCAAAA AAAGACAGAA 
GGAAAGGTTT CTACCAGTTC CAAGGAAACC AAGGAAAAAA ATCCAAACAA CTCTTCAAAT 
TGTCCTCCAT CTACAACAAC TCCTGACAGT TCCAAGTCCC GAAGAGCAAA GCTTGCTTTT 
GATGACAGGA GCTATTCAGC AGATCATTAC TTACAAGAAG CAAAAAAACT AAAGCACAAT 
GCAGATGCGT TGTCCGATAG GTTTGAGAAA GCGGTCTTCT ACCTTGATGC TGTTGTGTCT 
TTCATTGAAT GTGGGAATGC ATTGGAGAAA AACGCTCAGG AATCCAAATC CCCGTTCCCT 
ATGTATTCAG AAACTGTGGA ACTAATTAAA TACACTATGA AGCTGAAGAA TTACTTGGCA 
CCAGATGCCA CAGCTGCAGA TAAAAGACTA GCCGTGCTAT GTCTGCGATG CCAGTCCCTG 
TTGTACCTGA GGTTGTTCAA GCTGAAGAAA GAGAGTGCGT TGAAATACTC AAAAACACTG 
ACGGAACATC TAAAGAACTC CTATAATAAT TCGCAAGCTC CCTCACCTGG CATAGGAAGC 
AAAGCCGCCG GGATGCCCTC GCCTGTCTCC CCCAAGCTTT CTCCGGGCAA TTCAGGAAAC 
TACTCCTCAG GGGCCAGCAA CCCTTCGGGG AGCGGTTCTT CGGTGACAAT TCCGCAGAGG 
ATCCACCAGA TGGCGGCCAG CTACGTCCAG GTCACCTCCA ACTTCCTCTA CGCGACTGAA 
ATTTGGGACC AAGCCGAACA GCTTTCCAAA GAGCAAAAAG AATTCTTTGC TGAACTGGAT 
AAAGTTATGG GCCCGCTCAT CTTTAATTCA AGCATCATGA CAGACCTAGT TCGTTACACC 
CGGCAGGGAC TCCACTGGTT GCGTCAGGAT GCCAAGTCGA TACCTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

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AFF4 cDNA ORF clone, Ornithorhynchus anatinus(platypus)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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