crocc cDNA ORF clone, Oryzias latipes(Japanese medaka)

Gene
Clones
gRNAs

Summary

Gene Symbol	crocc
Entrez Gene ID	101159974
Full Name	ciliary rootlet coiled-coil, rootletin
Gene Type	protein-coding
Organism	Oryzias latipes(Japanese medaka)

mRNA and Protein(s)

mRNA	Protein	Name
XM_020701418.1	XP_020557077.1	rootletin isoform X1
XM_020701419.2	XP_020557078.1	rootletin isoform X2
XM_020701421.1	XP_020557080.1	rootletin isoform X4
XM_020701420.1	XP_020557079.1	rootletin isoform X3
XM_020701421.2	XP_020557080.1	rootletin isoform X4
XM_020701419.1	XP_020557078.1	rootletin isoform X2
XM_020701420.2	XP_020557079.1	rootletin isoform X3
XM_020701418.2	XP_020557077.1	rootletin isoform X1

Pathways from BioSystems
Gene Ontology

The following crocc gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the crocc cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OJa68720	XM_020701421.2 Latest version!	Oryzias latipes ciliary rootlet coiled-coil, rootletin (crocc), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OJa25264	XM_020701418.2 Latest version!	Oryzias latipes ciliary rootlet coiled-coil, rootletin (crocc), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OJa25265	XM_020701419.2 Latest version!	Oryzias latipes ciliary rootlet coiled-coil, rootletin (crocc), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OJa25266	XM_020701420.2 Latest version!	Oryzias latipes ciliary rootlet coiled-coil, rootletin (crocc), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
View Old Accession Versions >>
OJa68720	XM_020701421.1	Oryzias latipes ciliary rootlet coiled-coil, rootletin (crocc), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OJa25265	XM_020701419.1	Oryzias latipes ciliary rootlet coiled-coil, rootletin (crocc), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OJa25266	XM_020701420.1	Oryzias latipes ciliary rootlet coiled-coil, rootletin (crocc), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OJa25264	XM_020701418.1	Oryzias latipes ciliary rootlet coiled-coil, rootletin (crocc), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

crocc ( XM_020701421.1 ) cDNA ORF clone, Oryzias latipes(Japanese medaka) -> XP_020557080.1 Oryzias latipes ciliary rootlet coiled-coil, rootletin (crocc), transcript variant X4, mRNA.

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CloneID	OJa68720
Clone ID Related Accession (Same CDS sequence)	XM_020701421.1 , XM_020701421.2
Accession Version	XM_020701421.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5847bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1491408000000
Organism	Oryzias latipes(Japanese medaka)
Product	rootletin isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_004095157.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Oryzias latipes Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCTCGC TCATGTCCCT CCAGGAGGAG AACCGGGTGC TGCAGGGCGA GCTGGCCCGG 
CTGGAGGACC TGCTGGCGCA CAGCAGGGCG GATCGGGACG AGCTCGCCAT CAAGTACGGG 
GCAATCAGTG AACGGCTGGA GCAGGCTCTA CGCTCAGAAA CGGGGGAGGG AGAGGCCGAC 
TCACAGGCGT CCCGCAGCCT GGCTCAGCAG AATGTGGAAT TGCGCCGACG GCTGGACGAG 
GAACAGGCTG CATACAGGCG CAAACTCACC GCCTACCAAG AAGGCCAGCA GAGGCAGGCG 
CAGCTGGTGC AGAAGCTTCA GGCCAAGGTG CTTCAGTACA AAAAGAGATG TGGGGATCTG 
GAGCAGAAGC TGCAGGAGAA GTCCTCAGAG TTAGAGAAAT GCAGTGAGCG CAGTGAAACG 
TCTAACGGTC GCCATCACGA GGACTCGAGC AGCAGTTTGG AGGAGGCTTT ACTACACCTG 
GAGGAGGAGC AACAGAGGAG CAGCAGTTTA TCTGCAGTGA ACGCCATGCT GAGAGAACAG 
TTGGAGCAGG CCGGCTTGGC CAACGAGGCT CTGAGCCGGG ACATCCGGAG GCTCACCGCT 
GATTGGACGA AAGCCAGAGA GGAGCTGGAA CAAAAGGAGT CGGACTGGAG GAGAGAGGAG 
GAGTCTTTCC ACAGCTACTT CAGCAGTGAA CACGGTCGAC TGCTGACCTT TTGGCGACAG 
GTGGTGGGGT TCAGGAGGCA CGTCTGTGAG ATGAAGAGCA GCGCTGAGAG GGACCTGTCA 
GACATGCGGG GCGAGCTGGT TCGGATGTCC CACTTGGTCC AGGTGTCCTG CGCGGGTCTG 
TCCGCCGTGC TGCGCAGTCA GGAAGCAGGA ACGGCTCTAG CCCTGGAGCA GGAGAGAGCT 
CTGAGGGATC AGCTGGAGCA GCAGCTGAGA GGTCAAGTCA AAGAGACGAT GAACCTTCAG 
AGCAGAGCCG ATGCAGAGAG AAGTGAGCTG AGCCTCAGGC TTTCAGATGC GGCTCAAGAA 
AATGAAAGAC TAAAAAGTCA AATGGAAGAA AGAGACCGTG AGATCGACAA TCTGAGCAGG 
AGGCTTGAGG AGCAGAGCCG CAGCGATGAG ACGGACTCCC AGCTGCTGAG GGCTCATTGC 
AACGCTCTGT TGGACACGCT GAAGGACATC GCTCAGACGG TTCTGTCAGA CGGAGAGTCG 
TCTTCAGAGG CCGATCCGGA CAACTCGGCG GTCGTGCTGC AGGCGCTGAT CCGTGACTCC 
TCCCCTCAAC GCTCCTCCCA AACCAGCTCC TCGTTGTCCC CTCTCCCGGA AGCGGCGCTG 
TCTGCGCTGC GTTCGGTGGT CACAAACATG ATGCTGCAGC TGCAGGATGT TCGAGGTCGT 
CTGCTCTCTG CACAGTCCTC TGCGCAGAAG TTTCGCAAGC AGCTGTCAGA AATCGACTCT 
GCTAAAAGAG ACGCAGACCA ACGAAACCAA GCTCTGCAGC GAGACAAGGA CGCTGCTCTC 
AGAGAGCGGG AAACTGCCCA GAGGGAGAAG GAGCAACTGA AGCAAGAGAG AGACAAGCTG 
GCCAGTGAGA AGGCCAACCT GGAGAAGGCT GTTCAGGCGC TGCACAGCAG CAACCAGATG 
CTGCAGATGG ACTACGAGAA GCTCCAGCTG GCTGTGGCTT CTGCACAGCG AGAGCGAGAC 
CGCGAGAGGG AGGAGAAGGA GGCTGCCATC CATGACGGGG AGCGCTGGAA GACGGAGATT 
CAGAGGATAC AGAAGCAGAG GGACCAGAGT GAAAGTCGAG CCTCGGCTCA GCGGGGAGAA 
CTGTCTGCAG TGTTACAAAC TCACCAGCAG GGGGAGGTGG AGAGGCAGCT GCTGGAACAG 
GAGAGTGCCC ACCTTTCAGA GGCCCTTGCT CGGGCTGCAA GCACCAACAA AGAGCTGTCG 
CTGCATCTGA GCAAGCTGCA GTATGAAGAT GCAGCTCTCA GAGACTCTCT CACAAAGATG 
GGAAACCTGA ATGAGGGTCT GGTCCAGGAC AAGGTGGACC TTAACAAGTA TATTCTGCAG 
CTTGAGGAGG AGAAGGCCGT CCTACAGGCT CAGAAACGGG AGGCGGAGCA GGAGAAACTG 
TCCATCAGGG ACGAGCTGGT CCGGCAGGAG CAGAACCGAC TGGAGTTGGA GTCTTCCCGC 
GTCACACTGC AGCAGTCTCT GCAGGACACC GAGTTCAGTC GGGTGGGCGT GGAGGCAGAG 
CTGCAGAACC TCAGGGCTGA ACGACTGAAG CTACAGGAAA AGGTCGCCCA GTTGTGTGGT 
GAGGTGGCCT CTCTAGATTC AGAGTTGAGT CTCGCCAAGG GGGAGGGCCA GAGGAGCGAA 
ATGGCCTTGG AGGAAGCCGG CCGCAGTCGA GCAGAACTGA CCAAAGACAA AGCAGCTCTA 
GTGGTTCAGC TGACGGCATC TGAGAGAGAA AACGCAATGC TAGCCGAGGA ACTGACAGCT 
TTCAGGTCGG AGCGGGAGAC CCTGGAAACG AGTCTCTTTG ATGCGCAGCA GCAGCTTTTG 
CAGGCAGAGT CTTGCAGGGA ACAGCTGGAA GCAGAAAACC AAAACCTCCG CGTTCGCTGT 
GAAACTGCCA CAGTGGAGCT CAGGCGTTTG CGCTCAGATG CTGAGAATCA GCTGGCCCAG 
GCTGAGAGAG ACAAACAAGC GCTGACTCAA GCTCTCAGCG CCGCTCAGCT GGAGGCCCAG 
CAGGCTTCAC GCAAGGCCAC CTCCGAGCAT CAGGAGGAGG TGGAGAGACT GGCCTTAGAG 
AAGGAAGTCG TGCGGCACAG CTTGCAGATG GAGCACGAGA CGGCTGTCCG GAAACTCCGA 
CAAGAAGTCG AAGATCAGCT CCAGAAAGCC AGGAGAGAAA AAGAGGAGCT GCAGGACGGA 
CTGAGGGCTC TGCAGCACGA GCGAGATCAG AGCCTGCTGC AGGCGGAAAC GGAGAAACAA 
CAGGCCCTTT CACTGAAGGA GGCAGAGAAA ACGGTTTTGT TGGAGAAGGT GTCCGGTCTG 
CAGGCTGATC TGTCAGCGCT GAGCCTGGAG GCTGAGCGGA CAGCCAGAGA AGCTGCCCAG 
TACAAAGAGC AGGAGCAGAT CAGAGTTGGG GCTCTAACCA GCGAACTGCA AGAACTTCGG 
TCACAGATGG AAGATGCAGC GGCCGGTCAT GAAAGGGACA TCCTGAGTCT GAACGAAACC 
TGTGCAGACC TCCGTTCTCG TGCTGATGTT GCCCTCAAAG AGCTGGAGCA GTGCAGAGCT 
TCTCTCTCTG CTGCTGAGGA GAATCGAGAG CAGCTGAGGC GGGACTTCCT GGAGACGGAG 
CGCAGACTCA CCCAAACTCA GGAAGCGTCA GAAAACCACC GGAGGGAAGG AGCCGAGCTG 
CGGCGCAGCC TCTGTGATGT CACCAGGGAG AGAGATGCTC TCAGCCAATC AAATGCTCAG 
CTGAGGGAAG CTCTCCGAAG CGCAGAAACG GAGAGAATAA GCGTGAAACG GCAGTGTGAG 
GAGAAGGAGC AGCGGCTGGT TGTGCTGGAG GAGAGCCTGT CCTCCTCGCA GAAGGAGGTG 
GCAGAGCTGC GGAGTTGCCT GAGGGAGGTG GAAAGGTCCC GCCTTGAGGC TCGCCGTGAG 
CTTCAGGAGC TTCGGAGACA GCTGAAGGTT CTGGATGCAG ACAGAGAGCA GAAAGGGAGG 
GAGCTGGCGG AGCTGCAAAC CCGCTTGTCG TTGGAGGAGC AAAGAGAGGA GGAAAGAGGA 
AAGGAGGTTT TCATTTTAAA ACAGAAGCTC GCTGAAACCC AAACGGCCAG AGAGTCCCTC 
AAGAGAGAGC TCTCCATGAC ACAGAAGCGT CTGGTGGAGT CCGAGTCCGG CTGGCGCAGC 
TGTGAGCGGG AGCTGACGGC ACAGCTGCAG GAGGCGCGAG GCTGCGAGAA GAAGCTCCAG 
GATGAGGCCA AGAACCTGTC GGTGCGCGCG CAAGCGGCGC AGGAATCCGC CGCTCAGTGC 
AGCCTGCAGC TCAGCGAGGC GCAGGGGAGA CTGACCGCCA CCGAAGCTGA GCTGACGCGG 
GCGGAGGCCT CCAAGAGGGA TCTGGAGTTT CGCCTCAGCA GCCTCCAGTC GGCGCTGACG 
CGAACGCTGG GCATCGGGGC AAGCGGCAGG GCGAGCAGGG GGAGGAGTCC AGGCGGGAGC 
TCTGCATCAC CTGGCAGTAT CTCCCGTCAC CACAGCATCT CACCTTTGCG CCCTTCGCTG 
TCGCCCCCTA AAGAATTCAG AGGAAGCACG CCTGATAACA CCATGGGATC AGGAGCTGTG 
TCTCCTGAAG GAGGCGGTAC TCCTTTGCCT CCCGCTCAGC CAGAGCTGGA TCCCGACACG 
CTGCGCAGTG GCCTCAGAGA CTTCCTGCAG GAGCTGCGAG ACGCACACAG ACAAAGGGAT 
GATGCCCAAT GCCAGCTGAT GACTCTGCAG CGGGAGCTGG AGGAGCAGAA GCTGGAGCGA 
GAATGTGCCG AGAACCGCTC CACTCAGCTG CAAAACAGCC TGAAGGAATG CCAAGAAGGG 
AAGCGTGGAC TTGACGACCG TCTGACCAGC ACTCAGATTC TCCTGCAGCA GCAGGAGGAT 
GCGGTGAAGA GAGTGGACAG AGAGAGGAGA GCTCTTGCCG ACAGGGTGAG GGAGTTGGAG 
CGCTCGCTGC ACACCGCAGA GATGGAGAAG AAGCACACTC AGGAGCAGTT GAATAAGCAG 
CGGGCCACTG AGGCACGCCT GGAGGCGGAG AGGAAGCGCC TGCGGGAGGC TCTGGAGGCC 
GCCGAAGCTC GAGCCACCCG GGTGGAGCTG GGGAGGCGCA GCCTGGAAGG GGAGCTGCAG 
AGGCTCAAAC TCGGTCTGGG AGACCGGGAG GCTGAAAGCC AGGCCTCACA GGAGCGCCAC 
GACTGTTTGC TCAGACAGGT GGCGGAAGGA GAAGCACAAG TCTCTCTGCT GCAGACAGAG 
GTGGACAGAC TGAGTCAAGC TCTGCTGAAA GCTCAGGAAG GGGAAGCTGC TCTGAAGCAG 
AAGGTCACGA CTCTCAGCCA GAGCCTCCAG GAGGCTGCAG CTGATCACAG CAGCACGCAG 
ACTCGCCTCA CTGCTCTGCA GAAAACACTG AGCTTGGTGG AACAGGACAA AAGACTTCTG 
CAGGAGAAGA TGGATGAAGC CCGCACTTCT GTAACTGAAG CAAAGAGAAG CATGTTTGCT 
CTCACTGAGC GCGTGCAGAG CCTCCAGGGT GAGCTGACCC AGAGTGAACT GCTGAGAGAG 
GAGCTGGAGG CGGAGCTTAA GAACACACAG GAGGCACTCC GACAGCGCTC AGGAAGTTTG 
GCAGAGACTC AGCACAGCAT CCAGAAAGCT CAGACGGAGC GGGCAGCTGT GGAGGAGCGT 
CTGCTCGCAC TGCAAAGAGC TGTGGCTCTG CTGGAGACAG AGAAAAAAGA TGCAGAGAGG 
CAAGCTGCAA GGCTGGAGAA GGACAAGGAT GCACTGAGGA ACACGTTGGA TAAGGTTGAA 
AGGCAGAAAC TGAAGTGTGA GGAGGGCAGC ATGCGGCTCT CAGCAGAGAA GACCCGGCTG 
GACCGCTCCC TGAACGCAGT GGAGCAGGAG CTGCAGGAGG CGCAGCAGCA GATGCTGATG 
CTGCAGACTC AGCTGGCTGA GATGGAGCAG GCCCACAGTG GGTGTGAGGG TTTGCTGCGA 
CAGCGGGATG AAGCTCAGCA GGAGGCGGAG AAACTGAGGA ACAGCTGTAA GGACCTGGAA 
CGCACTCTGA GCACCAGAGA ACGAGCCCAC CGGCACCGAG TCAAAGGACT GGAGGAGCAG 
GTGTCCACTT TGAAGGAGCA GCTGCAGCAG GAGATGAAAC GGCGGCAGCC TCCTCTTCCG 
TCCACGTTTA CGTCCACTGG GATCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_020557080.1
CDS	480..6326
Translation	MSSLMSLQEENRVLQGELARLEDLLAHSRADRDELAIKYGAISERLEQALRSETGEGEADSQASRSLAQQNVELRRRLDEEQAAYRRKLTAYQEGQQRQAQLVQKLQAKVLQYKKRCGDLEQKLQEKSSELEKCSERSETSNGRHHEDSSSSLEEALLHLEEEQQRSSSLSAVNAMLREQLEQAGLANEALSRDIRRLTADWTKAREELEQKESDWRREEESFHSYFSSEHGRLLTFWRQVVGFRRHVCEMKSSAERDLSDMRGELVRMSHLVQVSCAGLSAVLRSQEAGTALALEQERALRDQLEQQLRGQVKETMNLQSRADAERSELSLRLSDAAQENERLKSQMEERDREIDNLSRRLEEQSRSDETDSQLLRAHCNALLDTLKDIAQTVLSDGESSSEADPDNSAVVLQALIRDSSPQRSSQTSSSLSPLPEAALSALRSVVTNMMLQLQDVRGRLLSAQSSAQKFRKQLSEIDSAKRDADQRNQALQRDKDAALRERETAQREKEQLKQERDKLASEKANLEKAVQALHSSNQMLQMDYEKLQLAVASAQRERDREREEKEAAIHDGERWKTEIQRIQKQRDQSESRASAQRGELSAVLQTHQQGEVERQLLEQESAHLSEALARAASTNKELSLHLSKLQYEDAALRDSLTKMGNLNEGLVQDKVDLNKYILQLEEEKAVLQAQKREAEQEKLSIRDELVRQEQNRLELESSRVTLQQSLQDTEFSRVGVEAELQNLRAERLKLQEKVAQLCGEVASLDSELSLAKGEGQRSEMALEEAGRSRAELTKDKAALVVQLTASERENAMLAEELTAFRSERETLETSLFDAQQQLLQAESCREQLEAENQNLRVRCETATVELRRLRSDAENQLAQAERDKQALTQALSAAQLEAQQASRKATSEHQEEVERLALEKEVVRHSLQMEHETAVRKLRQEVEDQLQKARREKEELQDGLRALQHERDQSLLQAETEKQQALSLKEAEKTVLLEKVSGLQADLSALSLEAERTAREAAQYKEQEQIRVGALTSELQELRSQMEDAAAGHERDILSLNETCADLRSRADVALKELEQCRASLSAAEENREQLRRDFLETERRLTQTQEASENHRREGAELRRSLCDVTRERDALSQSNAQLREALRSAETERISVKRQCEEKEQRLVVLEESLSSSQKEVAELRSCLREVERSRLEARRELQELRRQLKVLDADREQKGRELAELQTRLSLEEQREEERGKEVFILKQKLAETQTARESLKRELSMTQKRLVESESGWRSCERELTAQLQEARGCEKKLQDEAKNLSVRAQAAQESAAQCSLQLSEAQGRLTATEAELTRAEASKRDLEFRLSSLQSALTRTLGIGASGRASRGRSPGGSSASPGSISRHHSISPLRPSLSPPKEFRGSTPDNTMGSGAVSPEGGGTPLPPAQPELDPDTLRSGLRDFLQELRDAHRQRDDAQCQLMTLQRELEEQKLERECAENRSTQLQNSLKECQEGKRGLDDRLTSTQILLQQQEDAVKRVDRERRALADRVRELERSLHTAEMEKKHTQEQLNKQRATEARLEAERKRLREALEAAEARATRVELGRRSLEGELQRLKLGLGDREAESQASQERHDCLLRQVAEGEAQVSLLQTEVDRLSQALLKAQEGEAALKQKVTTLSQSLQEAAADHSSTQTRLTALQKTLSLVEQDKRLLQEKMDEARTSVTEAKRSMFALTERVQSLQGELTQSELLREELEAELKNTQEALRQRSGSLAETQHSIQKAQTERAAVEERLLALQRAVALLETEKKDAERQAARLEKDKDALRNTLDKVERQKLKCEEGSMRLSAEKTRLDRSLNAVEQELQEAQQQMLMLQTQLAEMEQAHSGCEGLLRQRDEAQQEAEKLRNSCKDLERTLSTRERAHRHRVKGLEEQVSTLKEQLQQEMKRRQPPLPSTFTSTGI

Target ORF information:

RefSeq Version	XM_020701421.1
Organism	Oryzias latipes(Japanese medaka)
Definition	Oryzias latipes ciliary rootlet coiled-coil, rootletin (crocc), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_020701421.1

ORF Insert Sequence:

ATGTCCTCGC TCATGTCCCT CCAGGAGGAG AACCGGGTGC TGCAGGGCGA GCTGGCCCGG 
CTGGAGGACC TGCTGGCGCA CAGCAGGGCG GATCGGGACG AGCTCGCCAT CAAGTACGGG 
GCAATCAGTG AACGGCTGGA GCAGGCTCTA CGCTCAGAAA CGGGGGAGGG AGAGGCCGAC 
TCACAGGCGT CCCGCAGCCT GGCTCAGCAG AATGTGGAAT TGCGCCGACG GCTGGACGAG 
GAACAGGCTG CATACAGGCG CAAACTCACC GCCTACCAAG AAGGCCAGCA GAGGCAGGCG 
CAGCTGGTGC AGAAGCTTCA GGCCAAGGTG CTTCAGTACA AAAAGAGATG TGGGGATCTG 
GAGCAGAAGC TGCAGGAGAA GTCCTCAGAG TTAGAGAAAT GCAGTGAGCG CAGTGAAACG 
TCTAACGGTC GCCATCACGA GGACTCGAGC AGCAGTTTGG AGGAGGCTTT ACTACACCTG 
GAGGAGGAGC AACAGAGGAG CAGCAGTTTA TCTGCAGTGA ACGCCATGCT GAGAGAACAG 
TTGGAGCAGG CCGGCTTGGC CAACGAGGCT CTGAGCCGGG ACATCCGGAG GCTCACCGCT 
GATTGGACGA AAGCCAGAGA GGAGCTGGAA CAAAAGGAGT CGGACTGGAG GAGAGAGGAG 
GAGTCTTTCC ACAGCTACTT CAGCAGTGAA CACGGTCGAC TGCTGACCTT TTGGCGACAG 
GTGGTGGGGT TCAGGAGGCA CGTCTGTGAG ATGAAGAGCA GCGCTGAGAG GGACCTGTCA 
GACATGCGGG GCGAGCTGGT TCGGATGTCC CACTTGGTCC AGGTGTCCTG CGCGGGTCTG 
TCCGCCGTGC TGCGCAGTCA GGAAGCAGGA ACGGCTCTAG CCCTGGAGCA GGAGAGAGCT 
CTGAGGGATC AGCTGGAGCA GCAGCTGAGA GGTCAAGTCA AAGAGACGAT GAACCTTCAG 
AGCAGAGCCG ATGCAGAGAG AAGTGAGCTG AGCCTCAGGC TTTCAGATGC GGCTCAAGAA 
AATGAAAGAC TAAAAAGTCA AATGGAAGAA AGAGACCGTG AGATCGACAA TCTGAGCAGG 
AGGCTTGAGG AGCAGAGCCG CAGCGATGAG ACGGACTCCC AGCTGCTGAG GGCTCATTGC 
AACGCTCTGT TGGACACGCT GAAGGACATC GCTCAGACGG TTCTGTCAGA CGGAGAGTCG 
TCTTCAGAGG CCGATCCGGA CAACTCGGCG GTCGTGCTGC AGGCGCTGAT CCGTGACTCC 
TCCCCTCAAC GCTCCTCCCA AACCAGCTCC TCGTTGTCCC CTCTCCCGGA AGCGGCGCTG 
TCTGCGCTGC GTTCGGTGGT CACAAACATG ATGCTGCAGC TGCAGGATGT TCGAGGTCGT 
CTGCTCTCTG CACAGTCCTC TGCGCAGAAG TTTCGCAAGC AGCTGTCAGA AATCGACTCT 
GCTAAAAGAG ACGCAGACCA ACGAAACCAA GCTCTGCAGC GAGACAAGGA CGCTGCTCTC 
AGAGAGCGGG AAACTGCCCA GAGGGAGAAG GAGCAACTGA AGCAAGAGAG AGACAAGCTG 
GCCAGTGAGA AGGCCAACCT GGAGAAGGCT GTTCAGGCGC TGCACAGCAG CAACCAGATG 
CTGCAGATGG ACTACGAGAA GCTCCAGCTG GCTGTGGCTT CTGCACAGCG AGAGCGAGAC 
CGCGAGAGGG AGGAGAAGGA GGCTGCCATC CATGACGGGG AGCGCTGGAA GACGGAGATT 
CAGAGGATAC AGAAGCAGAG GGACCAGAGT GAAAGTCGAG CCTCGGCTCA GCGGGGAGAA 
CTGTCTGCAG TGTTACAAAC TCACCAGCAG GGGGAGGTGG AGAGGCAGCT GCTGGAACAG 
GAGAGTGCCC ACCTTTCAGA GGCCCTTGCT CGGGCTGCAA GCACCAACAA AGAGCTGTCG 
CTGCATCTGA GCAAGCTGCA GTATGAAGAT GCAGCTCTCA GAGACTCTCT CACAAAGATG 
GGAAACCTGA ATGAGGGTCT GGTCCAGGAC AAGGTGGACC TTAACAAGTA TATTCTGCAG 
CTTGAGGAGG AGAAGGCCGT CCTACAGGCT CAGAAACGGG AGGCGGAGCA GGAGAAACTG 
TCCATCAGGG ACGAGCTGGT CCGGCAGGAG CAGAACCGAC TGGAGTTGGA GTCTTCCCGC 
GTCACACTGC AGCAGTCTCT GCAGGACACC GAGTTCAGTC GGGTGGGCGT GGAGGCAGAG 
CTGCAGAACC TCAGGGCTGA ACGACTGAAG CTACAGGAAA AGGTCGCCCA GTTGTGTGGT 
GAGGTGGCCT CTCTAGATTC AGAGTTGAGT CTCGCCAAGG GGGAGGGCCA GAGGAGCGAA 
ATGGCCTTGG AGGAAGCCGG CCGCAGTCGA GCAGAACTGA CCAAAGACAA AGCAGCTCTA 
GTGGTTCAGC TGACGGCATC TGAGAGAGAA AACGCAATGC TAGCCGAGGA ACTGACAGCT 
TTCAGGTCGG AGCGGGAGAC CCTGGAAACG AGTCTCTTTG ATGCGCAGCA GCAGCTTTTG 
CAGGCAGAGT CTTGCAGGGA ACAGCTGGAA GCAGAAAACC AAAACCTCCG CGTTCGCTGT 
GAAACTGCCA CAGTGGAGCT CAGGCGTTTG CGCTCAGATG CTGAGAATCA GCTGGCCCAG 
GCTGAGAGAG ACAAACAAGC GCTGACTCAA GCTCTCAGCG CCGCTCAGCT GGAGGCCCAG 
CAGGCTTCAC GCAAGGCCAC CTCCGAGCAT CAGGAGGAGG TGGAGAGACT GGCCTTAGAG 
AAGGAAGTCG TGCGGCACAG CTTGCAGATG GAGCACGAGA CGGCTGTCCG GAAACTCCGA 
CAAGAAGTCG AAGATCAGCT CCAGAAAGCC AGGAGAGAAA AAGAGGAGCT GCAGGACGGA 
CTGAGGGCTC TGCAGCACGA GCGAGATCAG AGCCTGCTGC AGGCGGAAAC GGAGAAACAA 
CAGGCCCTTT CACTGAAGGA GGCAGAGAAA ACGGTTTTGT TGGAGAAGGT GTCCGGTCTG 
CAGGCTGATC TGTCAGCGCT GAGCCTGGAG GCTGAGCGGA CAGCCAGAGA AGCTGCCCAG 
TACAAAGAGC AGGAGCAGAT CAGAGTTGGG GCTCTAACCA GCGAACTGCA AGAACTTCGG 
TCACAGATGG AAGATGCAGC GGCCGGTCAT GAAAGGGACA TCCTGAGTCT GAACGAAACC 
TGTGCAGACC TCCGTTCTCG TGCTGATGTT GCCCTCAAAG AGCTGGAGCA GTGCAGAGCT 
TCTCTCTCTG CTGCTGAGGA GAATCGAGAG CAGCTGAGGC GGGACTTCCT GGAGACGGAG 
CGCAGACTCA CCCAAACTCA GGAAGCGTCA GAAAACCACC GGAGGGAAGG AGCCGAGCTG 
CGGCGCAGCC TCTGTGATGT CACCAGGGAG AGAGATGCTC TCAGCCAATC AAATGCTCAG 
CTGAGGGAAG CTCTCCGAAG CGCAGAAACG GAGAGAATAA GCGTGAAACG GCAGTGTGAG 
GAGAAGGAGC AGCGGCTGGT TGTGCTGGAG GAGAGCCTGT CCTCCTCGCA GAAGGAGGTG 
GCAGAGCTGC GGAGTTGCCT GAGGGAGGTG GAAAGGTCCC GCCTTGAGGC TCGCCGTGAG 
CTTCAGGAGC TTCGGAGACA GCTGAAGGTT CTGGATGCAG ACAGAGAGCA GAAAGGGAGG 
GAGCTGGCGG AGCTGCAAAC CCGCTTGTCG TTGGAGGAGC AAAGAGAGGA GGAAAGAGGA 
AAGGAGGTTT TCATTTTAAA ACAGAAGCTC GCTGAAACCC AAACGGCCAG AGAGTCCCTC 
AAGAGAGAGC TCTCCATGAC ACAGAAGCGT CTGGTGGAGT CCGAGTCCGG CTGGCGCAGC 
TGTGAGCGGG AGCTGACGGC ACAGCTGCAG GAGGCGCGAG GCTGCGAGAA GAAGCTCCAG 
GATGAGGCCA AGAACCTGTC GGTGCGCGCG CAAGCGGCGC AGGAATCCGC CGCTCAGTGC 
AGCCTGCAGC TCAGCGAGGC GCAGGGGAGA CTGACCGCCA CCGAAGCTGA GCTGACGCGG 
GCGGAGGCCT CCAAGAGGGA TCTGGAGTTT CGCCTCAGCA GCCTCCAGTC GGCGCTGACG 
CGAACGCTGG GCATCGGGGC AAGCGGCAGG GCGAGCAGGG GGAGGAGTCC AGGCGGGAGC 
TCTGCATCAC CTGGCAGTAT CTCCCGTCAC CACAGCATCT CACCTTTGCG CCCTTCGCTG 
TCGCCCCCTA AAGAATTCAG AGGAAGCACG CCTGATAACA CCATGGGATC AGGAGCTGTG 
TCTCCTGAAG GAGGCGGTAC TCCTTTGCCT CCCGCTCAGC CAGAGCTGGA TCCCGACACG 
CTGCGCAGTG GCCTCAGAGA CTTCCTGCAG GAGCTGCGAG ACGCACACAG ACAAAGGGAT 
GATGCCCAAT GCCAGCTGAT GACTCTGCAG CGGGAGCTGG AGGAGCAGAA GCTGGAGCGA 
GAATGTGCCG AGAACCGCTC CACTCAGCTG CAAAACAGCC TGAAGGAATG CCAAGAAGGG 
AAGCGTGGAC TTGACGACCG TCTGACCAGC ACTCAGATTC TCCTGCAGCA GCAGGAGGAT 
GCGGTGAAGA GAGTGGACAG AGAGAGGAGA GCTCTTGCCG ACAGGGTGAG GGAGTTGGAG 
CGCTCGCTGC ACACCGCAGA GATGGAGAAG AAGCACACTC AGGAGCAGTT GAATAAGCAG 
CGGGCCACTG AGGCACGCCT GGAGGCGGAG AGGAAGCGCC TGCGGGAGGC TCTGGAGGCC 
GCCGAAGCTC GAGCCACCCG GGTGGAGCTG GGGAGGCGCA GCCTGGAAGG GGAGCTGCAG 
AGGCTCAAAC TCGGTCTGGG AGACCGGGAG GCTGAAAGCC AGGCCTCACA GGAGCGCCAC 
GACTGTTTGC TCAGACAGGT GGCGGAAGGA GAAGCACAAG TCTCTCTGCT GCAGACAGAG 
GTGGACAGAC TGAGTCAAGC TCTGCTGAAA GCTCAGGAAG GGGAAGCTGC TCTGAAGCAG 
AAGGTCACGA CTCTCAGCCA GAGCCTCCAG GAGGCTGCAG CTGATCACAG CAGCACGCAG 
ACTCGCCTCA CTGCTCTGCA GAAAACACTG AGCTTGGTGG AACAGGACAA AAGACTTCTG 
CAGGAGAAGA TGGATGAAGC CCGCACTTCT GTAACTGAAG CAAAGAGAAG CATGTTTGCT 
CTCACTGAGC GCGTGCAGAG CCTCCAGGGT GAGCTGACCC AGAGTGAACT GCTGAGAGAG 
GAGCTGGAGG CGGAGCTTAA GAACACACAG GAGGCACTCC GACAGCGCTC AGGAAGTTTG 
GCAGAGACTC AGCACAGCAT CCAGAAAGCT CAGACGGAGC GGGCAGCTGT GGAGGAGCGT 
CTGCTCGCAC TGCAAAGAGC TGTGGCTCTG CTGGAGACAG AGAAAAAAGA TGCAGAGAGG 
CAAGCTGCAA GGCTGGAGAA GGACAAGGAT GCACTGAGGA ACACGTTGGA TAAGGTTGAA 
AGGCAGAAAC TGAAGTGTGA GGAGGGCAGC ATGCGGCTCT CAGCAGAGAA GACCCGGCTG 
GACCGCTCCC TGAACGCAGT GGAGCAGGAG CTGCAGGAGG CGCAGCAGCA GATGCTGATG 
CTGCAGACTC AGCTGGCTGA GATGGAGCAG GCCCACAGTG GGTGTGAGGG TTTGCTGCGA 
CAGCGGGATG AAGCTCAGCA GGAGGCGGAG AAACTGAGGA ACAGCTGTAA GGACCTGGAA 
CGCACTCTGA GCACCAGAGA ACGAGCCCAC CGGCACCGAG TCAAAGGACT GGAGGAGCAG 
GTGTCCACTT TGAAGGAGCA GCTGCAGCAG GAGATGAAAC GGCGGCAGCC TCCTCTTCCG 
TCCACGTTTA CGTCCACTGG GATCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

crocc ( XM_020701421.2 ) cDNA ORF clone, Oryzias latipes(Japanese medaka) -> XP_020557080.1 Oryzias latipes ciliary rootlet coiled-coil, rootletin (crocc), transcript variant X4, mRNA.

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CloneID	OJa68720
Clone ID Related Accession (Same CDS sequence)	XM_020701421.1 , XM_020701421.2
Accession Version	XM_020701421.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5847bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1518537600000
Organism	Oryzias latipes(Japanese medaka)
Product	rootletin isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_019863.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Feb 15, 2018 this sequence version replaced XM_020701421.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Oryzias latipes Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCTCGC TCATGTCCCT CCAGGAGGAG AACCGGGTGC TGCAGGGCGA GCTGGCCCGG 
CTGGAGGACC TGCTGGCGCA CAGCAGGGCG GATCGGGACG AGCTCGCCAT CAAGTACGGG 
GCAATCAGTG AACGGCTGGA GCAGGCTCTA CGCTCAGAAA CGGGGGAGGG AGAGGCCGAC 
TCACAGGCGT CCCGCAGCCT GGCTCAGCAG AATGTGGAAT TGCGCCGACG GCTGGACGAG 
GAACAGGCTG CATACAGGCG CAAACTCACC GCCTACCAAG AAGGCCAGCA GAGGCAGGCG 
CAGCTGGTGC AGAAGCTTCA GGCCAAGGTG CTTCAGTACA AAAAGAGATG TGGGGATCTG 
GAGCAGAAGC TGCAGGAGAA GTCCTCAGAG TTAGAGAAAT GCAGTGAGCG CAGTGAAACG 
TCTAACGGTC GCCATCACGA GGACTCGAGC AGCAGTTTGG AGGAGGCTTT ACTACACCTG 
GAGGAGGAGC AACAGAGGAG CAGCAGTTTA TCTGCAGTGA ACGCCATGCT GAGAGAACAG 
TTGGAGCAGG CCGGCTTGGC CAACGAGGCT CTGAGCCGGG ACATCCGGAG GCTCACCGCT 
GATTGGACGA AAGCCAGAGA GGAGCTGGAA CAAAAGGAGT CGGACTGGAG GAGAGAGGAG 
GAGTCTTTCC ACAGCTACTT CAGCAGTGAA CACGGTCGAC TGCTGACCTT TTGGCGACAG 
GTGGTGGGGT TCAGGAGGCA CGTCTGTGAG ATGAAGAGCA GCGCTGAGAG GGACCTGTCA 
GACATGCGGG GCGAGCTGGT TCGGATGTCC CACTTGGTCC AGGTGTCCTG CGCGGGTCTG 
TCCGCCGTGC TGCGCAGTCA GGAAGCAGGA ACGGCTCTAG CCCTGGAGCA GGAGAGAGCT 
CTGAGGGATC AGCTGGAGCA GCAGCTGAGA GGTCAAGTCA AAGAGACGAT GAACCTTCAG 
AGCAGAGCCG ATGCAGAGAG AAGTGAGCTG AGCCTCAGGC TTTCAGATGC GGCTCAAGAA 
AATGAAAGAC TAAAAAGTCA AATGGAAGAA AGAGACCGTG AGATCGACAA TCTGAGCAGG 
AGGCTTGAGG AGCAGAGCCG CAGCGATGAG ACGGACTCCC AGCTGCTGAG GGCTCATTGC 
AACGCTCTGT TGGACACGCT GAAGGACATC GCTCAGACGG TTCTGTCAGA CGGAGAGTCG 
TCTTCAGAGG CCGATCCGGA CAACTCGGCG GTCGTGCTGC AGGCGCTGAT CCGTGACTCC 
TCCCCTCAAC GCTCCTCCCA AACCAGCTCC TCGTTGTCCC CTCTCCCGGA AGCGGCGCTG 
TCTGCGCTGC GTTCGGTGGT CACAAACATG ATGCTGCAGC TGCAGGATGT TCGAGGTCGT 
CTGCTCTCTG CACAGTCCTC TGCGCAGAAG TTTCGCAAGC AGCTGTCAGA AATCGACTCT 
GCTAAAAGAG ACGCAGACCA ACGAAACCAA GCTCTGCAGC GAGACAAGGA CGCTGCTCTC 
AGAGAGCGGG AAACTGCCCA GAGGGAGAAG GAGCAACTGA AGCAAGAGAG AGACAAGCTG 
GCCAGTGAGA AGGCCAACCT GGAGAAGGCT GTTCAGGCGC TGCACAGCAG CAACCAGATG 
CTGCAGATGG ACTACGAGAA GCTCCAGCTG GCTGTGGCTT CTGCACAGCG AGAGCGAGAC 
CGCGAGAGGG AGGAGAAGGA GGCTGCCATC CATGACGGGG AGCGCTGGAA GACGGAGATT 
CAGAGGATAC AGAAGCAGAG GGACCAGAGT GAAAGTCGAG CCTCGGCTCA GCGGGGAGAA 
CTGTCTGCAG TGTTACAAAC TCACCAGCAG GGGGAGGTGG AGAGGCAGCT GCTGGAACAG 
GAGAGTGCCC ACCTTTCAGA GGCCCTTGCT CGGGCTGCAA GCACCAACAA AGAGCTGTCG 
CTGCATCTGA GCAAGCTGCA GTATGAAGAT GCAGCTCTCA GAGACTCTCT CACAAAGATG 
GGAAACCTGA ATGAGGGTCT GGTCCAGGAC AAGGTGGACC TTAACAAGTA TATTCTGCAG 
CTTGAGGAGG AGAAGGCCGT CCTACAGGCT CAGAAACGGG AGGCGGAGCA GGAGAAACTG 
TCCATCAGGG ACGAGCTGGT CCGGCAGGAG CAGAACCGAC TGGAGTTGGA GTCTTCCCGC 
GTCACACTGC AGCAGTCTCT GCAGGACACC GAGTTCAGTC GGGTGGGCGT GGAGGCAGAG 
CTGCAGAACC TCAGGGCTGA ACGACTGAAG CTACAGGAAA AGGTCGCCCA GTTGTGTGGT 
GAGGTGGCCT CTCTAGATTC AGAGTTGAGT CTCGCCAAGG GGGAGGGCCA GAGGAGCGAA 
ATGGCCTTGG AGGAAGCCGG CCGCAGTCGA GCAGAACTGA CCAAAGACAA AGCAGCTCTA 
GTGGTTCAGC TGACGGCATC TGAGAGAGAA AACGCAATGC TAGCCGAGGA ACTGACAGCT 
TTCAGGTCGG AGCGGGAGAC CCTGGAAACG AGTCTCTTTG ATGCGCAGCA GCAGCTTTTG 
CAGGCAGAGT CTTGCAGGGA ACAGCTGGAA GCAGAAAACC AAAACCTCCG CGTTCGCTGT 
GAAACTGCCA CAGTGGAGCT CAGGCGTTTG CGCTCAGATG CTGAGAATCA GCTGGCCCAG 
GCTGAGAGAG ACAAACAAGC GCTGACTCAA GCTCTCAGCG CCGCTCAGCT GGAGGCCCAG 
CAGGCTTCAC GCAAGGCCAC CTCCGAGCAT CAGGAGGAGG TGGAGAGACT GGCCTTAGAG 
AAGGAAGTCG TGCGGCACAG CTTGCAGATG GAGCACGAGA CGGCTGTCCG GAAACTCCGA 
CAAGAAGTCG AAGATCAGCT CCAGAAAGCC AGGAGAGAAA AAGAGGAGCT GCAGGACGGA 
CTGAGGGCTC TGCAGCACGA GCGAGATCAG AGCCTGCTGC AGGCGGAAAC GGAGAAACAA 
CAGGCCCTTT CACTGAAGGA GGCAGAGAAA ACGGTTTTGT TGGAGAAGGT GTCCGGTCTG 
CAGGCTGATC TGTCAGCGCT GAGCCTGGAG GCTGAGCGGA CAGCCAGAGA AGCTGCCCAG 
TACAAAGAGC AGGAGCAGAT CAGAGTTGGG GCTCTAACCA GCGAACTGCA AGAACTTCGG 
TCACAGATGG AAGATGCAGC GGCCGGTCAT GAAAGGGACA TCCTGAGTCT GAACGAAACC 
TGTGCAGACC TCCGTTCTCG TGCTGATGTT GCCCTCAAAG AGCTGGAGCA GTGCAGAGCT 
TCTCTCTCTG CTGCTGAGGA GAATCGAGAG CAGCTGAGGC GGGACTTCCT GGAGACGGAG 
CGCAGACTCA CCCAAACTCA GGAAGCGTCA GAAAACCACC GGAGGGAAGG AGCCGAGCTG 
CGGCGCAGCC TCTGTGATGT CACCAGGGAG AGAGATGCTC TCAGCCAATC AAATGCTCAG 
CTGAGGGAAG CTCTCCGAAG CGCAGAAACG GAGAGAATAA GCGTGAAACG GCAGTGTGAG 
GAGAAGGAGC AGCGGCTGGT TGTGCTGGAG GAGAGCCTGT CCTCCTCGCA GAAGGAGGTG 
GCAGAGCTGC GGAGTTGCCT GAGGGAGGTG GAAAGGTCCC GCCTTGAGGC TCGCCGTGAG 
CTTCAGGAGC TTCGGAGACA GCTGAAGGTT CTGGATGCAG ACAGAGAGCA GAAAGGGAGG 
GAGCTGGCGG AGCTGCAAAC CCGCTTGTCG TTGGAGGAGC AAAGAGAGGA GGAAAGAGGA 
AAGGAGGTTT TCATTTTAAA ACAGAAGCTC GCTGAAACCC AAACGGCCAG AGAGTCCCTC 
AAGAGAGAGC TCTCCATGAC ACAGAAGCGT CTGGTGGAGT CCGAGTCCGG CTGGCGCAGC 
TGTGAGCGGG AGCTGACGGC ACAGCTGCAG GAGGCGCGAG GCTGCGAGAA GAAGCTCCAG 
GATGAGGCCA AGAACCTGTC GGTGCGCGCG CAAGCGGCGC AGGAATCCGC CGCTCAGTGC 
AGCCTGCAGC TCAGCGAGGC GCAGGGGAGA CTGACCGCCA CCGAAGCTGA GCTGACGCGG 
GCGGAGGCCT CCAAGAGGGA TCTGGAGTTT CGCCTCAGCA GCCTCCAGTC GGCGCTGACG 
CGAACGCTGG GCATCGGGGC AAGCGGCAGG GCGAGCAGGG GGAGGAGTCC AGGCGGGAGC 
TCTGCATCAC CTGGCAGTAT CTCCCGTCAC CACAGCATCT CACCTTTGCG CCCTTCGCTG 
TCGCCCCCTA AAGAATTCAG AGGAAGCACG CCTGATAACA CCATGGGATC AGGAGCTGTG 
TCTCCTGAAG GAGGCGGTAC TCCTTTGCCT CCCGCTCAGC CAGAGCTGGA TCCCGACACG 
CTGCGCAGTG GCCTCAGAGA CTTCCTGCAG GAGCTGCGAG ACGCACACAG ACAAAGGGAT 
GATGCCCAAT GCCAGCTGAT GACTCTGCAG CGGGAGCTGG AGGAGCAGAA GCTGGAGCGA 
GAATGTGCCG AGAACCGCTC CACTCAGCTG CAAAACAGCC TGAAGGAATG CCAAGAAGGG 
AAGCGTGGAC TTGACGACCG TCTGACCAGC ACTCAGATTC TCCTGCAGCA GCAGGAGGAT 
GCGGTGAAGA GAGTGGACAG AGAGAGGAGA GCTCTTGCCG ACAGGGTGAG GGAGTTGGAG 
CGCTCGCTGC ACACCGCAGA GATGGAGAAG AAGCACACTC AGGAGCAGTT GAATAAGCAG 
CGGGCCACTG AGGCACGCCT GGAGGCGGAG AGGAAGCGCC TGCGGGAGGC TCTGGAGGCC 
GCCGAAGCTC GAGCCACCCG GGTGGAGCTG GGGAGGCGCA GCCTGGAAGG GGAGCTGCAG 
AGGCTCAAAC TCGGTCTGGG AGACCGGGAG GCTGAAAGCC AGGCCTCACA GGAGCGCCAC 
GACTGTTTGC TCAGACAGGT GGCGGAAGGA GAAGCACAAG TCTCTCTGCT GCAGACAGAG 
GTGGACAGAC TGAGTCAAGC TCTGCTGAAA GCTCAGGAAG GGGAAGCTGC TCTGAAGCAG 
AAGGTCACGA CTCTCAGCCA GAGCCTCCAG GAGGCTGCAG CTGATCACAG CAGCACGCAG 
ACTCGCCTCA CTGCTCTGCA GAAAACACTG AGCTTGGTGG AACAGGACAA AAGACTTCTG 
CAGGAGAAGA TGGATGAAGC CCGCACTTCT GTAACTGAAG CAAAGAGAAG CATGTTTGCT 
CTCACTGAGC GCGTGCAGAG CCTCCAGGGT GAGCTGACCC AGAGTGAACT GCTGAGAGAG 
GAGCTGGAGG CGGAGCTTAA GAACACACAG GAGGCACTCC GACAGCGCTC AGGAAGTTTG 
GCAGAGACTC AGCACAGCAT CCAGAAAGCT CAGACGGAGC GGGCAGCTGT GGAGGAGCGT 
CTGCTCGCAC TGCAAAGAGC TGTGGCTCTG CTGGAGACAG AGAAAAAAGA TGCAGAGAGG 
CAAGCTGCAA GGCTGGAGAA GGACAAGGAT GCACTGAGGA ACACGTTGGA TAAGGTTGAA 
AGGCAGAAAC TGAAGTGTGA GGAGGGCAGC ATGCGGCTCT CAGCAGAGAA GACCCGGCTG 
GACCGCTCCC TGAACGCAGT GGAGCAGGAG CTGCAGGAGG CGCAGCAGCA GATGCTGATG 
CTGCAGACTC AGCTGGCTGA GATGGAGCAG GCCCACAGTG GGTGTGAGGG TTTGCTGCGA 
CAGCGGGATG AAGCTCAGCA GGAGGCGGAG AAACTGAGGA ACAGCTGTAA GGACCTGGAA 
CGCACTCTGA GCACCAGAGA ACGAGCCCAC CGGCACCGAG TCAAAGGACT GGAGGAGCAG 
GTGTCCACTT TGAAGGAGCA GCTGCAGCAG GAGATGAAAC GGCGGCAGCC TCCTCTTCCG 
TCCACGTTTA CGTCCACTGG GATCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_020557080.1
CDS	484..6330
Translation	MSSLMSLQEENRVLQGELARLEDLLAHSRADRDELAIKYGAISERLEQALRSETGEGEADSQASRSLAQQNVELRRRLDEEQAAYRRKLTAYQEGQQRQAQLVQKLQAKVLQYKKRCGDLEQKLQEKSSELEKCSERSETSNGRHHEDSSSSLEEALLHLEEEQQRSSSLSAVNAMLREQLEQAGLANEALSRDIRRLTADWTKAREELEQKESDWRREEESFHSYFSSEHGRLLTFWRQVVGFRRHVCEMKSSAERDLSDMRGELVRMSHLVQVSCAGLSAVLRSQEAGTALALEQERALRDQLEQQLRGQVKETMNLQSRADAERSELSLRLSDAAQENERLKSQMEERDREIDNLSRRLEEQSRSDETDSQLLRAHCNALLDTLKDIAQTVLSDGESSSEADPDNSAVVLQALIRDSSPQRSSQTSSSLSPLPEAALSALRSVVTNMMLQLQDVRGRLLSAQSSAQKFRKQLSEIDSAKRDADQRNQALQRDKDAALRERETAQREKEQLKQERDKLASEKANLEKAVQALHSSNQMLQMDYEKLQLAVASAQRERDREREEKEAAIHDGERWKTEIQRIQKQRDQSESRASAQRGELSAVLQTHQQGEVERQLLEQESAHLSEALARAASTNKELSLHLSKLQYEDAALRDSLTKMGNLNEGLVQDKVDLNKYILQLEEEKAVLQAQKREAEQEKLSIRDELVRQEQNRLELESSRVTLQQSLQDTEFSRVGVEAELQNLRAERLKLQEKVAQLCGEVASLDSELSLAKGEGQRSEMALEEAGRSRAELTKDKAALVVQLTASERENAMLAEELTAFRSERETLETSLFDAQQQLLQAESCREQLEAENQNLRVRCETATVELRRLRSDAENQLAQAERDKQALTQALSAAQLEAQQASRKATSEHQEEVERLALEKEVVRHSLQMEHETAVRKLRQEVEDQLQKARREKEELQDGLRALQHERDQSLLQAETEKQQALSLKEAEKTVLLEKVSGLQADLSALSLEAERTAREAAQYKEQEQIRVGALTSELQELRSQMEDAAAGHERDILSLNETCADLRSRADVALKELEQCRASLSAAEENREQLRRDFLETERRLTQTQEASENHRREGAELRRSLCDVTRERDALSQSNAQLREALRSAETERISVKRQCEEKEQRLVVLEESLSSSQKEVAELRSCLREVERSRLEARRELQELRRQLKVLDADREQKGRELAELQTRLSLEEQREEERGKEVFILKQKLAETQTARESLKRELSMTQKRLVESESGWRSCERELTAQLQEARGCEKKLQDEAKNLSVRAQAAQESAAQCSLQLSEAQGRLTATEAELTRAEASKRDLEFRLSSLQSALTRTLGIGASGRASRGRSPGGSSASPGSISRHHSISPLRPSLSPPKEFRGSTPDNTMGSGAVSPEGGGTPLPPAQPELDPDTLRSGLRDFLQELRDAHRQRDDAQCQLMTLQRELEEQKLERECAENRSTQLQNSLKECQEGKRGLDDRLTSTQILLQQQEDAVKRVDRERRALADRVRELERSLHTAEMEKKHTQEQLNKQRATEARLEAERKRLREALEAAEARATRVELGRRSLEGELQRLKLGLGDREAESQASQERHDCLLRQVAEGEAQVSLLQTEVDRLSQALLKAQEGEAALKQKVTTLSQSLQEAAADHSSTQTRLTALQKTLSLVEQDKRLLQEKMDEARTSVTEAKRSMFALTERVQSLQGELTQSELLREELEAELKNTQEALRQRSGSLAETQHSIQKAQTERAAVEERLLALQRAVALLETEKKDAERQAARLEKDKDALRNTLDKVERQKLKCEEGSMRLSAEKTRLDRSLNAVEQELQEAQQQMLMLQTQLAEMEQAHSGCEGLLRQRDEAQQEAEKLRNSCKDLERTLSTRERAHRHRVKGLEEQVSTLKEQLQQEMKRRQPPLPSTFTSTGI

Target ORF information:

RefSeq Version	XM_020701421.2
Organism	Oryzias latipes(Japanese medaka)
Definition	Oryzias latipes ciliary rootlet coiled-coil, rootletin (crocc), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_020701421.2

ORF Insert Sequence:

ATGTCCTCGC TCATGTCCCT CCAGGAGGAG AACCGGGTGC TGCAGGGCGA GCTGGCCCGG 
CTGGAGGACC TGCTGGCGCA CAGCAGGGCG GATCGGGACG AGCTCGCCAT CAAGTACGGG 
GCAATCAGTG AACGGCTGGA GCAGGCTCTA CGCTCAGAAA CGGGGGAGGG AGAGGCCGAC 
TCACAGGCGT CCCGCAGCCT GGCTCAGCAG AATGTGGAAT TGCGCCGACG GCTGGACGAG 
GAACAGGCTG CATACAGGCG CAAACTCACC GCCTACCAAG AAGGCCAGCA GAGGCAGGCG 
CAGCTGGTGC AGAAGCTTCA GGCCAAGGTG CTTCAGTACA AAAAGAGATG TGGGGATCTG 
GAGCAGAAGC TGCAGGAGAA GTCCTCAGAG TTAGAGAAAT GCAGTGAGCG CAGTGAAACG 
TCTAACGGTC GCCATCACGA GGACTCGAGC AGCAGTTTGG AGGAGGCTTT ACTACACCTG 
GAGGAGGAGC AACAGAGGAG CAGCAGTTTA TCTGCAGTGA ACGCCATGCT GAGAGAACAG 
TTGGAGCAGG CCGGCTTGGC CAACGAGGCT CTGAGCCGGG ACATCCGGAG GCTCACCGCT 
GATTGGACGA AAGCCAGAGA GGAGCTGGAA CAAAAGGAGT CGGACTGGAG GAGAGAGGAG 
GAGTCTTTCC ACAGCTACTT CAGCAGTGAA CACGGTCGAC TGCTGACCTT TTGGCGACAG 
GTGGTGGGGT TCAGGAGGCA CGTCTGTGAG ATGAAGAGCA GCGCTGAGAG GGACCTGTCA 
GACATGCGGG GCGAGCTGGT TCGGATGTCC CACTTGGTCC AGGTGTCCTG CGCGGGTCTG 
TCCGCCGTGC TGCGCAGTCA GGAAGCAGGA ACGGCTCTAG CCCTGGAGCA GGAGAGAGCT 
CTGAGGGATC AGCTGGAGCA GCAGCTGAGA GGTCAAGTCA AAGAGACGAT GAACCTTCAG 
AGCAGAGCCG ATGCAGAGAG AAGTGAGCTG AGCCTCAGGC TTTCAGATGC GGCTCAAGAA 
AATGAAAGAC TAAAAAGTCA AATGGAAGAA AGAGACCGTG AGATCGACAA TCTGAGCAGG 
AGGCTTGAGG AGCAGAGCCG CAGCGATGAG ACGGACTCCC AGCTGCTGAG GGCTCATTGC 
AACGCTCTGT TGGACACGCT GAAGGACATC GCTCAGACGG TTCTGTCAGA CGGAGAGTCG 
TCTTCAGAGG CCGATCCGGA CAACTCGGCG GTCGTGCTGC AGGCGCTGAT CCGTGACTCC 
TCCCCTCAAC GCTCCTCCCA AACCAGCTCC TCGTTGTCCC CTCTCCCGGA AGCGGCGCTG 
TCTGCGCTGC GTTCGGTGGT CACAAACATG ATGCTGCAGC TGCAGGATGT TCGAGGTCGT 
CTGCTCTCTG CACAGTCCTC TGCGCAGAAG TTTCGCAAGC AGCTGTCAGA AATCGACTCT 
GCTAAAAGAG ACGCAGACCA ACGAAACCAA GCTCTGCAGC GAGACAAGGA CGCTGCTCTC 
AGAGAGCGGG AAACTGCCCA GAGGGAGAAG GAGCAACTGA AGCAAGAGAG AGACAAGCTG 
GCCAGTGAGA AGGCCAACCT GGAGAAGGCT GTTCAGGCGC TGCACAGCAG CAACCAGATG 
CTGCAGATGG ACTACGAGAA GCTCCAGCTG GCTGTGGCTT CTGCACAGCG AGAGCGAGAC 
CGCGAGAGGG AGGAGAAGGA GGCTGCCATC CATGACGGGG AGCGCTGGAA GACGGAGATT 
CAGAGGATAC AGAAGCAGAG GGACCAGAGT GAAAGTCGAG CCTCGGCTCA GCGGGGAGAA 
CTGTCTGCAG TGTTACAAAC TCACCAGCAG GGGGAGGTGG AGAGGCAGCT GCTGGAACAG 
GAGAGTGCCC ACCTTTCAGA GGCCCTTGCT CGGGCTGCAA GCACCAACAA AGAGCTGTCG 
CTGCATCTGA GCAAGCTGCA GTATGAAGAT GCAGCTCTCA GAGACTCTCT CACAAAGATG 
GGAAACCTGA ATGAGGGTCT GGTCCAGGAC AAGGTGGACC TTAACAAGTA TATTCTGCAG 
CTTGAGGAGG AGAAGGCCGT CCTACAGGCT CAGAAACGGG AGGCGGAGCA GGAGAAACTG 
TCCATCAGGG ACGAGCTGGT CCGGCAGGAG CAGAACCGAC TGGAGTTGGA GTCTTCCCGC 
GTCACACTGC AGCAGTCTCT GCAGGACACC GAGTTCAGTC GGGTGGGCGT GGAGGCAGAG 
CTGCAGAACC TCAGGGCTGA ACGACTGAAG CTACAGGAAA AGGTCGCCCA GTTGTGTGGT 
GAGGTGGCCT CTCTAGATTC AGAGTTGAGT CTCGCCAAGG GGGAGGGCCA GAGGAGCGAA 
ATGGCCTTGG AGGAAGCCGG CCGCAGTCGA GCAGAACTGA CCAAAGACAA AGCAGCTCTA 
GTGGTTCAGC TGACGGCATC TGAGAGAGAA AACGCAATGC TAGCCGAGGA ACTGACAGCT 
TTCAGGTCGG AGCGGGAGAC CCTGGAAACG AGTCTCTTTG ATGCGCAGCA GCAGCTTTTG 
CAGGCAGAGT CTTGCAGGGA ACAGCTGGAA GCAGAAAACC AAAACCTCCG CGTTCGCTGT 
GAAACTGCCA CAGTGGAGCT CAGGCGTTTG CGCTCAGATG CTGAGAATCA GCTGGCCCAG 
GCTGAGAGAG ACAAACAAGC GCTGACTCAA GCTCTCAGCG CCGCTCAGCT GGAGGCCCAG 
CAGGCTTCAC GCAAGGCCAC CTCCGAGCAT CAGGAGGAGG TGGAGAGACT GGCCTTAGAG 
AAGGAAGTCG TGCGGCACAG CTTGCAGATG GAGCACGAGA CGGCTGTCCG GAAACTCCGA 
CAAGAAGTCG AAGATCAGCT CCAGAAAGCC AGGAGAGAAA AAGAGGAGCT GCAGGACGGA 
CTGAGGGCTC TGCAGCACGA GCGAGATCAG AGCCTGCTGC AGGCGGAAAC GGAGAAACAA 
CAGGCCCTTT CACTGAAGGA GGCAGAGAAA ACGGTTTTGT TGGAGAAGGT GTCCGGTCTG 
CAGGCTGATC TGTCAGCGCT GAGCCTGGAG GCTGAGCGGA CAGCCAGAGA AGCTGCCCAG 
TACAAAGAGC AGGAGCAGAT CAGAGTTGGG GCTCTAACCA GCGAACTGCA AGAACTTCGG 
TCACAGATGG AAGATGCAGC GGCCGGTCAT GAAAGGGACA TCCTGAGTCT GAACGAAACC 
TGTGCAGACC TCCGTTCTCG TGCTGATGTT GCCCTCAAAG AGCTGGAGCA GTGCAGAGCT 
TCTCTCTCTG CTGCTGAGGA GAATCGAGAG CAGCTGAGGC GGGACTTCCT GGAGACGGAG 
CGCAGACTCA CCCAAACTCA GGAAGCGTCA GAAAACCACC GGAGGGAAGG AGCCGAGCTG 
CGGCGCAGCC TCTGTGATGT CACCAGGGAG AGAGATGCTC TCAGCCAATC AAATGCTCAG 
CTGAGGGAAG CTCTCCGAAG CGCAGAAACG GAGAGAATAA GCGTGAAACG GCAGTGTGAG 
GAGAAGGAGC AGCGGCTGGT TGTGCTGGAG GAGAGCCTGT CCTCCTCGCA GAAGGAGGTG 
GCAGAGCTGC GGAGTTGCCT GAGGGAGGTG GAAAGGTCCC GCCTTGAGGC TCGCCGTGAG 
CTTCAGGAGC TTCGGAGACA GCTGAAGGTT CTGGATGCAG ACAGAGAGCA GAAAGGGAGG 
GAGCTGGCGG AGCTGCAAAC CCGCTTGTCG TTGGAGGAGC AAAGAGAGGA GGAAAGAGGA 
AAGGAGGTTT TCATTTTAAA ACAGAAGCTC GCTGAAACCC AAACGGCCAG AGAGTCCCTC 
AAGAGAGAGC TCTCCATGAC ACAGAAGCGT CTGGTGGAGT CCGAGTCCGG CTGGCGCAGC 
TGTGAGCGGG AGCTGACGGC ACAGCTGCAG GAGGCGCGAG GCTGCGAGAA GAAGCTCCAG 
GATGAGGCCA AGAACCTGTC GGTGCGCGCG CAAGCGGCGC AGGAATCCGC CGCTCAGTGC 
AGCCTGCAGC TCAGCGAGGC GCAGGGGAGA CTGACCGCCA CCGAAGCTGA GCTGACGCGG 
GCGGAGGCCT CCAAGAGGGA TCTGGAGTTT CGCCTCAGCA GCCTCCAGTC GGCGCTGACG 
CGAACGCTGG GCATCGGGGC AAGCGGCAGG GCGAGCAGGG GGAGGAGTCC AGGCGGGAGC 
TCTGCATCAC CTGGCAGTAT CTCCCGTCAC CACAGCATCT CACCTTTGCG CCCTTCGCTG 
TCGCCCCCTA AAGAATTCAG AGGAAGCACG CCTGATAACA CCATGGGATC AGGAGCTGTG 
TCTCCTGAAG GAGGCGGTAC TCCTTTGCCT CCCGCTCAGC CAGAGCTGGA TCCCGACACG 
CTGCGCAGTG GCCTCAGAGA CTTCCTGCAG GAGCTGCGAG ACGCACACAG ACAAAGGGAT 
GATGCCCAAT GCCAGCTGAT GACTCTGCAG CGGGAGCTGG AGGAGCAGAA GCTGGAGCGA 
GAATGTGCCG AGAACCGCTC CACTCAGCTG CAAAACAGCC TGAAGGAATG CCAAGAAGGG 
AAGCGTGGAC TTGACGACCG TCTGACCAGC ACTCAGATTC TCCTGCAGCA GCAGGAGGAT 
GCGGTGAAGA GAGTGGACAG AGAGAGGAGA GCTCTTGCCG ACAGGGTGAG GGAGTTGGAG 
CGCTCGCTGC ACACCGCAGA GATGGAGAAG AAGCACACTC AGGAGCAGTT GAATAAGCAG 
CGGGCCACTG AGGCACGCCT GGAGGCGGAG AGGAAGCGCC TGCGGGAGGC TCTGGAGGCC 
GCCGAAGCTC GAGCCACCCG GGTGGAGCTG GGGAGGCGCA GCCTGGAAGG GGAGCTGCAG 
AGGCTCAAAC TCGGTCTGGG AGACCGGGAG GCTGAAAGCC AGGCCTCACA GGAGCGCCAC 
GACTGTTTGC TCAGACAGGT GGCGGAAGGA GAAGCACAAG TCTCTCTGCT GCAGACAGAG 
GTGGACAGAC TGAGTCAAGC TCTGCTGAAA GCTCAGGAAG GGGAAGCTGC TCTGAAGCAG 
AAGGTCACGA CTCTCAGCCA GAGCCTCCAG GAGGCTGCAG CTGATCACAG CAGCACGCAG 
ACTCGCCTCA CTGCTCTGCA GAAAACACTG AGCTTGGTGG AACAGGACAA AAGACTTCTG 
CAGGAGAAGA TGGATGAAGC CCGCACTTCT GTAACTGAAG CAAAGAGAAG CATGTTTGCT 
CTCACTGAGC GCGTGCAGAG CCTCCAGGGT GAGCTGACCC AGAGTGAACT GCTGAGAGAG 
GAGCTGGAGG CGGAGCTTAA GAACACACAG GAGGCACTCC GACAGCGCTC AGGAAGTTTG 
GCAGAGACTC AGCACAGCAT CCAGAAAGCT CAGACGGAGC GGGCAGCTGT GGAGGAGCGT 
CTGCTCGCAC TGCAAAGAGC TGTGGCTCTG CTGGAGACAG AGAAAAAAGA TGCAGAGAGG 
CAAGCTGCAA GGCTGGAGAA GGACAAGGAT GCACTGAGGA ACACGTTGGA TAAGGTTGAA 
AGGCAGAAAC TGAAGTGTGA GGAGGGCAGC ATGCGGCTCT CAGCAGAGAA GACCCGGCTG 
GACCGCTCCC TGAACGCAGT GGAGCAGGAG CTGCAGGAGG CGCAGCAGCA GATGCTGATG 
CTGCAGACTC AGCTGGCTGA GATGGAGCAG GCCCACAGTG GGTGTGAGGG TTTGCTGCGA 
CAGCGGGATG AAGCTCAGCA GGAGGCGGAG AAACTGAGGA ACAGCTGTAA GGACCTGGAA 
CGCACTCTGA GCACCAGAGA ACGAGCCCAC CGGCACCGAG TCAAAGGACT GGAGGAGCAG 
GTGTCCACTT TGAAGGAGCA GCTGCAGCAG GAGATGAAAC GGCGGCAGCC TCCTCTTCCG 
TCCACGTTTA CGTCCACTGG GATCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

crocc ( XM_020701418.1 ) cDNA ORF clone, Oryzias latipes(Japanese medaka) -> XP_020557077.1 Oryzias latipes ciliary rootlet coiled-coil, rootletin (crocc), transcript variant X1, mRNA.

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CloneID	OJa25264
Clone ID Related Accession (Same CDS sequence)	XM_020701418.1 , XM_020701418.2
Accession Version	XM_020701418.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6039bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1491408000000
Organism	Oryzias latipes(Japanese medaka)
Product	rootletin isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_004095157.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Oryzias latipes Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCGCTG CGGAAAACTC CGACAGCGAC CCCAGCAAGC TGGAGTCAGT GATCCGGAGG 
CTGGAGGAAA GTGTTCTGTC TGAGGAGAAG AGGTTGACGG TTCGAGGCCC TTCACCGGAT 
GACCCCCCTG CGTGTCTGCC TGCCAGAGTG CGAGAGATCG TCACGAAGAA CCTCAGTGAC 
AGCACAGGGG AGATGTCCTC GCTCATGTCC CTCCAGGAGG AGAACCGGGT GCTGCAGGGC 
GAGCTGGCCC GGCTGGAGGA CCTGCTGGCG CACAGCAGGG CGGATCGGGA CGAGCTCGCC 
ATCAAGTACG GGGCAATCAG TGAACGGCTG GAGCAGGCTC TACGCTCAGA AACGGGGGAG 
GGAGAGGCCG ACTCACAGGC GTCCCGCAGC CTGGCTCAGC AGAATGTGGA ATTGCGCCGA 
CGGCTGGACG AGGAACAGGC TGCATACAGG CGCAAACTCA CCGCCTACCA AGAAGGCCAG 
CAGAGGCAGG CGCAGCTGGT GCAGAAGCTT CAGGCCAAGG TGCTTCAGTA CAAAAAGAGA 
TGTGGGGATC TGGAGCAGAA GCTGCAGGAG AAGTCCTCAG AGTTAGAGAA ATGCAGTGAG 
CGCAGTGAAA CGTCTAACGG TCGCCATCAC GAGGACTCGA GCAGCAGTTT GGAGGAGGCT 
TTACTACACC TGGAGGAGGA GCAACAGAGG AGCAGCAGTT TATCTGCAGT GAACGCCATG 
CTGAGAGAAC AGTTGGAGCA GGCCGGCTTG GCCAACGAGG CTCTGAGCCG GGACATCCGG 
AGGCTCACCG CTGATTGGAC GAAAGCCAGA GAGGAGCTGG AACAAAAGGA GTCGGACTGG 
AGGAGAGAGG AGGAGTCTTT CCACAGCTAC TTCAGCAGTG AACACGGTCG ACTGCTGACC 
TTTTGGCGAC AGGTGGTGGG GTTCAGGAGG CACGTCTGTG AGATGAAGAG CAGCGCTGAG 
AGGGACCTGT CAGACATGCG GGGCGAGCTG GTTCGGATGT CCCACTTGGT CCAGGTGTCC 
TGCGCGGGTC TGTCCGCCGT GCTGCGCAGT CAGGAAGCAG GAACGGCTCT AGCCCTGGAG 
CAGGAGAGAG CTCTGAGGGA TCAGCTGGAG CAGCAGCTGA GAGGTCAAGT CAAAGAGACG 
ATGAACCTTC AGAGCAGAGC CGATGCAGAG AGAAGTGAGC TGAGCCTCAG GCTTTCAGAT 
GCGGCTCAAG AAAATGAAAG ACTAAAAAGT CAAATGGAAG AAAGAGACCG TGAGATCGAC 
AATCTGAGCA GGAGGCTTGA GGAGCAGAGC CGCAGCGATG AGACGGACTC CCAGCTGCTG 
AGGGCTCATT GCAACGCTCT GTTGGACACG CTGAAGGACA TCGCTCAGAC GGTTCTGTCA 
GACGGAGAGT CGTCTTCAGA GGCCGATCCG GACAACTCGG CGGTCGTGCT GCAGGCGCTG 
ATCCGTGACT CCTCCCCTCA ACGCTCCTCC CAAACCAGCT CCTCGTTGTC CCCTCTCCCG 
GAAGCGGCGC TGTCTGCGCT GCGTTCGGTG GTCACAAACA TGATGCTGCA GCTGCAGGAT 
GTTCGAGGTC GTCTGCTCTC TGCACAGTCC TCTGCGCAGA AGTTTCGCAA GCAGCTGTCA 
GAAATCGACT CTGCTAAAAG AGACGCAGAC CAACGAAACC AAGCTCTGCA GCGAGACAAG 
GACGCTGCTC TCAGAGAGCG GGAAACTGCC CAGAGGGAGA AGGAGCAACT GAAGCAAGAG 
AGAGACAAGC TGGCCAGTGA GAAGGCCAAC CTGGAGAAGG CTGTTCAGGC GCTGCACAGC 
AGCAACCAGA TGCTGCAGAT GGACTACGAG AAGCTCCAGC TGGCTGTGGC TTCTGCACAG 
CGAGAGCGAG ACCGCGAGAG GGAGGAGAAG GAGGCTGCCA TCCATGACGG GGAGCGCTGG 
AAGACGGAGA TTCAGAGGAT ACAGAAGCAG AGGGACCAGA GTGAAAGTCG AGCCTCGGCT 
CAGCGGGGAG AACTGTCTGC AGTGTTACAA ACTCACCAGC AGGGGGAGGT GGAGAGGCAG 
CTGCTGGAAC AGGAGAGTGC CCACCTTTCA GAGGCCCTTG CTCGGGCTGC AAGCACCAAC 
AAAGAGCTGT CGCTGCATCT GAGCAAGCTG CAGTATGAAG ATGCAGCTCT CAGAGACTCT 
CTCACAAAGA TGGGAAACCT GAATGAGGGT CTGGTCCAGG ACAAGGTGGA CCTTAACAAG 
TATATTCTGC AGCTTGAGGA GGAGAAGGCC GTCCTACAGG CTCAGAAACG GGAGGCGGAG 
CAGGAGAAAC TGTCCATCAG GGACGAGCTG GTCCGGCAGG AGCAGAACCG ACTGGAGTTG 
GAGTCTTCCC GCGTCACACT GCAGCAGTCT CTGCAGGACA CCGAGTTCAG TCGGGTGGGC 
GTGGAGGCAG AGCTGCAGAA CCTCAGGGCT GAACGACTGA AGCTACAGGA AAAGGTCGCC 
CAGTTGTGTG GTGAGGTGGC CTCTCTAGAT TCAGAGTTGA GTCTCGCCAA GGGGGAGGGC 
CAGAGGAGCG AAATGGCCTT GGAGGAAGCC GGCCGCAGTC GAGCAGAACT GACCAAAGAC 
AAAGCAGCTC TAGTGGTTCA GCTGACGGCA TCTGAGAGAG AAAACGCAAT GCTAGCCGAG 
GAACTGACAG CTTTCAGGTC GGAGCGGGAG ACCCTGGAAA CGAGTCTCTT TGATGCGCAG 
CAGCAGCTTT TGCAGGCAGA GTCTTGCAGG GAACAGCTGG AAGCAGAAAA CCAAAACCTC 
CGCGTTCGCT GTGAAACTGC CACAGTGGAG CTCAGGCGTT TGCGCTCAGA TGCTGAGAAT 
CAGCTGGCCC AGGCTGAGAG AGACAAACAA GCGCTGACTC AAGCTCTCAG CGCCGCTCAG 
CTGGAGGCCC AGCAGGCTTC ACGCAAGGCC ACCTCCGAGC ATCAGGAGGA GGTGGAGAGA 
CTGGCCTTAG AGAAGGAAGT CGTGCGGCAC AGCTTGCAGA TGGAGCACGA GACGGCTGTC 
CGGAAACTCC GACAAGAAGT CGAAGATCAG CTCCAGAAAG CCAGGAGAGA AAAAGAGGAG 
CTGCAGGACG GACTGAGGGC TCTGCAGCAC GAGCGAGATC AGAGCCTGCT GCAGGCGGAA 
ACGGAGAAAC AACAGGCCCT TTCACTGAAG GAGGCAGAGA AAACGGTTTT GTTGGAGAAG 
GTGTCCGGTC TGCAGGCTGA TCTGTCAGCG CTGAGCCTGG AGGCTGAGCG GACAGCCAGA 
GAAGCTGCCC AGTACAAAGA GCAGGAGCAG ATCAGAGTTG GGGCTCTAAC CAGCGAACTG 
CAAGAACTTC GGTCACAGAT GGAAGATGCA GCGGCCGGTC ATGAAAGGGA CATCCTGAGT 
CTGAACGAAA CCTGTGCAGA CCTCCGTTCT CGTGCTGATG TTGCCCTCAA AGAGCTGGAG 
CAGTGCAGAG CTTCTCTCTC TGCTGCTGAG GAGAATCGAG AGCAGCTGAG GCGGGACTTC 
CTGGAGACGG AGCGCAGACT CACCCAAACT CAGGAAGCGT CAGAAAACCA CCGGAGGGAA 
GGAGCCGAGC TGCGGCGCAG CCTCTGTGAT GTCACCAGGG AGAGAGATGC TCTCAGCCAA 
TCAAATGCTC AGCTGAGGGA AGCTCTCCGA AGCGCAGAAA CGGAGAGAAT AAGCGTGAAA 
CGGCAGTGTG AGGAGAAGGA GCAGCGGCTG GTTGTGCTGG AGGAGAGCCT GTCCTCCTCG 
CAGAAGGAGG TGGCAGAGCT GCGGAGTTGC CTGAGGGAGG TGGAAAGGTC CCGCCTTGAG 
GCTCGCCGTG AGCTTCAGGA GCTTCGGAGA CAGCTGAAGG TTCTGGATGC AGACAGAGAG 
CAGAAAGGGA GGGAGCTGGC GGAGCTGCAA ACCCGCTTGT CGTTGGAGGA GCAAAGAGAG 
GAGGAAAGAG GAAAGGAGGT TTTCATTTTA AAACAGAAGC TCGCTGAAAC CCAAACGGCC 
AGAGAGTCCC TCAAGAGAGA GCTCTCCATG ACACAGAAGC GTCTGGTGGA GTCCGAGTCC 
GGCTGGCGCA GCTGTGAGCG GGAGCTGACG GCACAGCTGC AGGAGGCGCG AGGCTGCGAG 
AAGAAGCTCC AGGATGAGGC CAAGAACCTG TCGGTGCGCG CGCAAGCGGC GCAGGAATCC 
GCCGCTCAGT GCAGCCTGCA GCTCAGCGAG GCGCAGGGGA GACTGACCGC CACCGAAGCT 
GAGCTGACGC GGGCGGAGGC CTCCAAGAGG GATCTGGAGT TTCGCCTCAG CAGCCTCCAG 
TCGGCGCTGA CGCGAACGCT GGGCATCGGG GCAAGCGGCA GGGCGAGCAG GGGGAGGAGT 
CCAGGCGGGA GCTCTGCATC ACCTGGCAGT ATCTCCCGTC ACCACAGCAT CTCACCTTTG 
CGCCCTTCGC TGTCGCCCCC TAAAGAATTC AGAGGAAGCA CGCCTGATAA CACCATGGGA 
TCAGGAGCTG TGTCTCCTGA AGGAGGCGGT ACTCCTTTGC CTCCCGCTCA GCCAGAGCTG 
GATCCCGACA CGCTGCGCAG TGGCCTCAGA GACTTCCTGC AGGAGCTGCG AGACGCACAC 
AGACAAAGGG ATGATGCCCA ATGCCAGCTG ATGACTCTGC AGCGGGAGCT GGAGGAGCAG 
AAGCTGGAGC GAGAATGTGC CGAGAACCGC TCCACTCAGC TGCAAAACAG CCTGAAGGAA 
TGCCAAGAAG GGAAGCGTGG ACTTGACGAC CGTCTGACCA GCACTCAGAT TCTCCTGCAG 
CAGCAGGAGG ATGCGGTGAA GAGAGTGGAC AGAGAGAGGA GAGCTCTTGC CGACAGGGTG 
AGGGAGTTGG AGCGCTCGCT GCACACCGCA GAGATGGAGA AGAAGCACAC TCAGGAGCAG 
TTGAATAAGC AGCGGGCCAC TGAGGCACGC CTGGAGGCGG AGAGGAAGCG CCTGCGGGAG 
GCTCTGGAGG CCGCCGAAGC TCGAGCCACC CGGGTGGAGC TGGGGAGGCG CAGCCTGGAA 
GGGGAGCTGC AGAGGCTCAA ACTCGGTCTG GGAGACCGGG AGGCTGAAAG CCAGGCCTCA 
CAGGAGCGCC ACGACTGTTT GCTCAGACAG GTGGCGGAAG GAGAAGCACA AGTCTCTCTG 
CTGCAGACAG AGGTGGACAG ACTGAGTCAA GCTCTGCTGA AAGCTCAGGA AGGGGAAGCT 
GCTCTGAAGC AGAAGGTCAC GACTCTCAGC CAGAGCCTCC AGGAGGCTGC AGCTGATCAC 
AGCAGCACGC AGACTCGCCT CACTGCTCTG CAGAAAACAC TGAGCTTGGT GGAACAGGAC 
AAAAGACTTC TGCAGGAGAA GATGGATGAA GCCCGCACTT CTGTAACTGA AGCAAAGAGA 
AGCATGTTTG CTCTCACTGA GCGCGTGCAG AGCCTCCAGG GTGAGCTGAC CCAGAGTGAA 
CTGCTGAGAG AGGAGCTGGA GGCGGAGCTT AAGAACACAC AGGAGGCACT CCGACAGCGC 
TCAGGAAGTT TGGCAGAGAC TCAGCACAGC ATCCAGAAAG CTCAGACGGA GCGGGCAGCT 
GTGGAGGAGC GTCTGCTCGC ACTGCAAAGA GCTGTGGCTC TGCTGGAGAC AGAGAAAAAA 
GATGCAGAGA GGCAAGCTGC AAGGCTGGAG AAGGACAAGG ATGCACTGAG GAACACGTTG 
GATAAGGTTG AAAGGCAGAA ACTGAAGTGT GAGGAGGGCA GCATGCGGCT CTCAGCAGAG 
AAGACCCGGC TGGACCGCTC CCTGAACGCA GTGGAGCAGG AGCTGCAGGA GGCGCAGCAG 
CAGATGCTGA TGCTGCAGAC TCAGCTGGCT GAGATGGAGC AGGCCCACAG TGGGTGTGAG 
GGTTTGCTGC GACAGCGGGA TGAAGCTCAG CAGGAGGCGG AGAAACTGAG GAACAGCTGT 
AAGGACCTGG AACGCACTCT GAGCACCAGA GAACGAGCCC ACCGGCACCG AGTCAAAGGA 
CTGGAGGAGC AGGTGTCCAC TTTGAAGGAG CAGCTGCAGC AGGAGATGAA ACGGCGGCAG 
CCTCCTCTTC CGTCCACGTT TACGTCCACT GGGATCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_020557077.1
CDS	188..6226
Translation	MSAAENSDSDPSKLESVIRRLEESVLSEEKRLTVRGPSPDDPPACLPARVREIVTKNLSDSTGEMSSLMSLQEENRVLQGELARLEDLLAHSRADRDELAIKYGAISERLEQALRSETGEGEADSQASRSLAQQNVELRRRLDEEQAAYRRKLTAYQEGQQRQAQLVQKLQAKVLQYKKRCGDLEQKLQEKSSELEKCSERSETSNGRHHEDSSSSLEEALLHLEEEQQRSSSLSAVNAMLREQLEQAGLANEALSRDIRRLTADWTKAREELEQKESDWRREEESFHSYFSSEHGRLLTFWRQVVGFRRHVCEMKSSAERDLSDMRGELVRMSHLVQVSCAGLSAVLRSQEAGTALALEQERALRDQLEQQLRGQVKETMNLQSRADAERSELSLRLSDAAQENERLKSQMEERDREIDNLSRRLEEQSRSDETDSQLLRAHCNALLDTLKDIAQTVLSDGESSSEADPDNSAVVLQALIRDSSPQRSSQTSSSLSPLPEAALSALRSVVTNMMLQLQDVRGRLLSAQSSAQKFRKQLSEIDSAKRDADQRNQALQRDKDAALRERETAQREKEQLKQERDKLASEKANLEKAVQALHSSNQMLQMDYEKLQLAVASAQRERDREREEKEAAIHDGERWKTEIQRIQKQRDQSESRASAQRGELSAVLQTHQQGEVERQLLEQESAHLSEALARAASTNKELSLHLSKLQYEDAALRDSLTKMGNLNEGLVQDKVDLNKYILQLEEEKAVLQAQKREAEQEKLSIRDELVRQEQNRLELESSRVTLQQSLQDTEFSRVGVEAELQNLRAERLKLQEKVAQLCGEVASLDSELSLAKGEGQRSEMALEEAGRSRAELTKDKAALVVQLTASERENAMLAEELTAFRSERETLETSLFDAQQQLLQAESCREQLEAENQNLRVRCETATVELRRLRSDAENQLAQAERDKQALTQALSAAQLEAQQASRKATSEHQEEVERLALEKEVVRHSLQMEHETAVRKLRQEVEDQLQKARREKEELQDGLRALQHERDQSLLQAETEKQQALSLKEAEKTVLLEKVSGLQADLSALSLEAERTAREAAQYKEQEQIRVGALTSELQELRSQMEDAAAGHERDILSLNETCADLRSRADVALKELEQCRASLSAAEENREQLRRDFLETERRLTQTQEASENHRREGAELRRSLCDVTRERDALSQSNAQLREALRSAETERISVKRQCEEKEQRLVVLEESLSSSQKEVAELRSCLREVERSRLEARRELQELRRQLKVLDADREQKGRELAELQTRLSLEEQREEERGKEVFILKQKLAETQTARESLKRELSMTQKRLVESESGWRSCERELTAQLQEARGCEKKLQDEAKNLSVRAQAAQESAAQCSLQLSEAQGRLTATEAELTRAEASKRDLEFRLSSLQSALTRTLGIGASGRASRGRSPGGSSASPGSISRHHSISPLRPSLSPPKEFRGSTPDNTMGSGAVSPEGGGTPLPPAQPELDPDTLRSGLRDFLQELRDAHRQRDDAQCQLMTLQRELEEQKLERECAENRSTQLQNSLKECQEGKRGLDDRLTSTQILLQQQEDAVKRVDRERRALADRVRELERSLHTAEMEKKHTQEQLNKQRATEARLEAERKRLREALEAAEARATRVELGRRSLEGELQRLKLGLGDREAESQASQERHDCLLRQVAEGEAQVSLLQTEVDRLSQALLKAQEGEAALKQKVTTLSQSLQEAAADHSSTQTRLTALQKTLSLVEQDKRLLQEKMDEARTSVTEAKRSMFALTERVQSLQGELTQSELLREELEAELKNTQEALRQRSGSLAETQHSIQKAQTERAAVEERLLALQRAVALLETEKKDAERQAARLEKDKDALRNTLDKVERQKLKCEEGSMRLSAEKTRLDRSLNAVEQELQEAQQQMLMLQTQLAEMEQAHSGCEGLLRQRDEAQQEAEKLRNSCKDLERTLSTRERAHRHRVKGLEEQVSTLKEQLQQEMKRRQPPLPSTFTSTGI

Target ORF information:

RefSeq Version	XM_020701418.1
Organism	Oryzias latipes(Japanese medaka)
Definition	Oryzias latipes ciliary rootlet coiled-coil, rootletin (crocc), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_020701418.1

ORF Insert Sequence:

ATGAGCGCTG CGGAAAACTC CGACAGCGAC CCCAGCAAGC TGGAGTCAGT GATCCGGAGG 
CTGGAGGAAA GTGTTCTGTC TGAGGAGAAG AGGTTGACGG TTCGAGGCCC TTCACCGGAT 
GACCCCCCTG CGTGTCTGCC TGCCAGAGTG CGAGAGATCG TCACGAAGAA CCTCAGTGAC 
AGCACAGGGG AGATGTCCTC GCTCATGTCC CTCCAGGAGG AGAACCGGGT GCTGCAGGGC 
GAGCTGGCCC GGCTGGAGGA CCTGCTGGCG CACAGCAGGG CGGATCGGGA CGAGCTCGCC 
ATCAAGTACG GGGCAATCAG TGAACGGCTG GAGCAGGCTC TACGCTCAGA AACGGGGGAG 
GGAGAGGCCG ACTCACAGGC GTCCCGCAGC CTGGCTCAGC AGAATGTGGA ATTGCGCCGA 
CGGCTGGACG AGGAACAGGC TGCATACAGG CGCAAACTCA CCGCCTACCA AGAAGGCCAG 
CAGAGGCAGG CGCAGCTGGT GCAGAAGCTT CAGGCCAAGG TGCTTCAGTA CAAAAAGAGA 
TGTGGGGATC TGGAGCAGAA GCTGCAGGAG AAGTCCTCAG AGTTAGAGAA ATGCAGTGAG 
CGCAGTGAAA CGTCTAACGG TCGCCATCAC GAGGACTCGA GCAGCAGTTT GGAGGAGGCT 
TTACTACACC TGGAGGAGGA GCAACAGAGG AGCAGCAGTT TATCTGCAGT GAACGCCATG 
CTGAGAGAAC AGTTGGAGCA GGCCGGCTTG GCCAACGAGG CTCTGAGCCG GGACATCCGG 
AGGCTCACCG CTGATTGGAC GAAAGCCAGA GAGGAGCTGG AACAAAAGGA GTCGGACTGG 
AGGAGAGAGG AGGAGTCTTT CCACAGCTAC TTCAGCAGTG AACACGGTCG ACTGCTGACC 
TTTTGGCGAC AGGTGGTGGG GTTCAGGAGG CACGTCTGTG AGATGAAGAG CAGCGCTGAG 
AGGGACCTGT CAGACATGCG GGGCGAGCTG GTTCGGATGT CCCACTTGGT CCAGGTGTCC 
TGCGCGGGTC TGTCCGCCGT GCTGCGCAGT CAGGAAGCAG GAACGGCTCT AGCCCTGGAG 
CAGGAGAGAG CTCTGAGGGA TCAGCTGGAG CAGCAGCTGA GAGGTCAAGT CAAAGAGACG 
ATGAACCTTC AGAGCAGAGC CGATGCAGAG AGAAGTGAGC TGAGCCTCAG GCTTTCAGAT 
GCGGCTCAAG AAAATGAAAG ACTAAAAAGT CAAATGGAAG AAAGAGACCG TGAGATCGAC 
AATCTGAGCA GGAGGCTTGA GGAGCAGAGC CGCAGCGATG AGACGGACTC CCAGCTGCTG 
AGGGCTCATT GCAACGCTCT GTTGGACACG CTGAAGGACA TCGCTCAGAC GGTTCTGTCA 
GACGGAGAGT CGTCTTCAGA GGCCGATCCG GACAACTCGG CGGTCGTGCT GCAGGCGCTG 
ATCCGTGACT CCTCCCCTCA ACGCTCCTCC CAAACCAGCT CCTCGTTGTC CCCTCTCCCG 
GAAGCGGCGC TGTCTGCGCT GCGTTCGGTG GTCACAAACA TGATGCTGCA GCTGCAGGAT 
GTTCGAGGTC GTCTGCTCTC TGCACAGTCC TCTGCGCAGA AGTTTCGCAA GCAGCTGTCA 
GAAATCGACT CTGCTAAAAG AGACGCAGAC CAACGAAACC AAGCTCTGCA GCGAGACAAG 
GACGCTGCTC TCAGAGAGCG GGAAACTGCC CAGAGGGAGA AGGAGCAACT GAAGCAAGAG 
AGAGACAAGC TGGCCAGTGA GAAGGCCAAC CTGGAGAAGG CTGTTCAGGC GCTGCACAGC 
AGCAACCAGA TGCTGCAGAT GGACTACGAG AAGCTCCAGC TGGCTGTGGC TTCTGCACAG 
CGAGAGCGAG ACCGCGAGAG GGAGGAGAAG GAGGCTGCCA TCCATGACGG GGAGCGCTGG 
AAGACGGAGA TTCAGAGGAT ACAGAAGCAG AGGGACCAGA GTGAAAGTCG AGCCTCGGCT 
CAGCGGGGAG AACTGTCTGC AGTGTTACAA ACTCACCAGC AGGGGGAGGT GGAGAGGCAG 
CTGCTGGAAC AGGAGAGTGC CCACCTTTCA GAGGCCCTTG CTCGGGCTGC AAGCACCAAC 
AAAGAGCTGT CGCTGCATCT GAGCAAGCTG CAGTATGAAG ATGCAGCTCT CAGAGACTCT 
CTCACAAAGA TGGGAAACCT GAATGAGGGT CTGGTCCAGG ACAAGGTGGA CCTTAACAAG 
TATATTCTGC AGCTTGAGGA GGAGAAGGCC GTCCTACAGG CTCAGAAACG GGAGGCGGAG 
CAGGAGAAAC TGTCCATCAG GGACGAGCTG GTCCGGCAGG AGCAGAACCG ACTGGAGTTG 
GAGTCTTCCC GCGTCACACT GCAGCAGTCT CTGCAGGACA CCGAGTTCAG TCGGGTGGGC 
GTGGAGGCAG AGCTGCAGAA CCTCAGGGCT GAACGACTGA AGCTACAGGA AAAGGTCGCC 
CAGTTGTGTG GTGAGGTGGC CTCTCTAGAT TCAGAGTTGA GTCTCGCCAA GGGGGAGGGC 
CAGAGGAGCG AAATGGCCTT GGAGGAAGCC GGCCGCAGTC GAGCAGAACT GACCAAAGAC 
AAAGCAGCTC TAGTGGTTCA GCTGACGGCA TCTGAGAGAG AAAACGCAAT GCTAGCCGAG 
GAACTGACAG CTTTCAGGTC GGAGCGGGAG ACCCTGGAAA CGAGTCTCTT TGATGCGCAG 
CAGCAGCTTT TGCAGGCAGA GTCTTGCAGG GAACAGCTGG AAGCAGAAAA CCAAAACCTC 
CGCGTTCGCT GTGAAACTGC CACAGTGGAG CTCAGGCGTT TGCGCTCAGA TGCTGAGAAT 
CAGCTGGCCC AGGCTGAGAG AGACAAACAA GCGCTGACTC AAGCTCTCAG CGCCGCTCAG 
CTGGAGGCCC AGCAGGCTTC ACGCAAGGCC ACCTCCGAGC ATCAGGAGGA GGTGGAGAGA 
CTGGCCTTAG AGAAGGAAGT CGTGCGGCAC AGCTTGCAGA TGGAGCACGA GACGGCTGTC 
CGGAAACTCC GACAAGAAGT CGAAGATCAG CTCCAGAAAG CCAGGAGAGA AAAAGAGGAG 
CTGCAGGACG GACTGAGGGC TCTGCAGCAC GAGCGAGATC AGAGCCTGCT GCAGGCGGAA 
ACGGAGAAAC AACAGGCCCT TTCACTGAAG GAGGCAGAGA AAACGGTTTT GTTGGAGAAG 
GTGTCCGGTC TGCAGGCTGA TCTGTCAGCG CTGAGCCTGG AGGCTGAGCG GACAGCCAGA 
GAAGCTGCCC AGTACAAAGA GCAGGAGCAG ATCAGAGTTG GGGCTCTAAC CAGCGAACTG 
CAAGAACTTC GGTCACAGAT GGAAGATGCA GCGGCCGGTC ATGAAAGGGA CATCCTGAGT 
CTGAACGAAA CCTGTGCAGA CCTCCGTTCT CGTGCTGATG TTGCCCTCAA AGAGCTGGAG 
CAGTGCAGAG CTTCTCTCTC TGCTGCTGAG GAGAATCGAG AGCAGCTGAG GCGGGACTTC 
CTGGAGACGG AGCGCAGACT CACCCAAACT CAGGAAGCGT CAGAAAACCA CCGGAGGGAA 
GGAGCCGAGC TGCGGCGCAG CCTCTGTGAT GTCACCAGGG AGAGAGATGC TCTCAGCCAA 
TCAAATGCTC AGCTGAGGGA AGCTCTCCGA AGCGCAGAAA CGGAGAGAAT AAGCGTGAAA 
CGGCAGTGTG AGGAGAAGGA GCAGCGGCTG GTTGTGCTGG AGGAGAGCCT GTCCTCCTCG 
CAGAAGGAGG TGGCAGAGCT GCGGAGTTGC CTGAGGGAGG TGGAAAGGTC CCGCCTTGAG 
GCTCGCCGTG AGCTTCAGGA GCTTCGGAGA CAGCTGAAGG TTCTGGATGC AGACAGAGAG 
CAGAAAGGGA GGGAGCTGGC GGAGCTGCAA ACCCGCTTGT CGTTGGAGGA GCAAAGAGAG 
GAGGAAAGAG GAAAGGAGGT TTTCATTTTA AAACAGAAGC TCGCTGAAAC CCAAACGGCC 
AGAGAGTCCC TCAAGAGAGA GCTCTCCATG ACACAGAAGC GTCTGGTGGA GTCCGAGTCC 
GGCTGGCGCA GCTGTGAGCG GGAGCTGACG GCACAGCTGC AGGAGGCGCG AGGCTGCGAG 
AAGAAGCTCC AGGATGAGGC CAAGAACCTG TCGGTGCGCG CGCAAGCGGC GCAGGAATCC 
GCCGCTCAGT GCAGCCTGCA GCTCAGCGAG GCGCAGGGGA GACTGACCGC CACCGAAGCT 
GAGCTGACGC GGGCGGAGGC CTCCAAGAGG GATCTGGAGT TTCGCCTCAG CAGCCTCCAG 
TCGGCGCTGA CGCGAACGCT GGGCATCGGG GCAAGCGGCA GGGCGAGCAG GGGGAGGAGT 
CCAGGCGGGA GCTCTGCATC ACCTGGCAGT ATCTCCCGTC ACCACAGCAT CTCACCTTTG 
CGCCCTTCGC TGTCGCCCCC TAAAGAATTC AGAGGAAGCA CGCCTGATAA CACCATGGGA 
TCAGGAGCTG TGTCTCCTGA AGGAGGCGGT ACTCCTTTGC CTCCCGCTCA GCCAGAGCTG 
GATCCCGACA CGCTGCGCAG TGGCCTCAGA GACTTCCTGC AGGAGCTGCG AGACGCACAC 
AGACAAAGGG ATGATGCCCA ATGCCAGCTG ATGACTCTGC AGCGGGAGCT GGAGGAGCAG 
AAGCTGGAGC GAGAATGTGC CGAGAACCGC TCCACTCAGC TGCAAAACAG CCTGAAGGAA 
TGCCAAGAAG GGAAGCGTGG ACTTGACGAC CGTCTGACCA GCACTCAGAT TCTCCTGCAG 
CAGCAGGAGG ATGCGGTGAA GAGAGTGGAC AGAGAGAGGA GAGCTCTTGC CGACAGGGTG 
AGGGAGTTGG AGCGCTCGCT GCACACCGCA GAGATGGAGA AGAAGCACAC TCAGGAGCAG 
TTGAATAAGC AGCGGGCCAC TGAGGCACGC CTGGAGGCGG AGAGGAAGCG CCTGCGGGAG 
GCTCTGGAGG CCGCCGAAGC TCGAGCCACC CGGGTGGAGC TGGGGAGGCG CAGCCTGGAA 
GGGGAGCTGC AGAGGCTCAA ACTCGGTCTG GGAGACCGGG AGGCTGAAAG CCAGGCCTCA 
CAGGAGCGCC ACGACTGTTT GCTCAGACAG GTGGCGGAAG GAGAAGCACA AGTCTCTCTG 
CTGCAGACAG AGGTGGACAG ACTGAGTCAA GCTCTGCTGA AAGCTCAGGA AGGGGAAGCT 
GCTCTGAAGC AGAAGGTCAC GACTCTCAGC CAGAGCCTCC AGGAGGCTGC AGCTGATCAC 
AGCAGCACGC AGACTCGCCT CACTGCTCTG CAGAAAACAC TGAGCTTGGT GGAACAGGAC 
AAAAGACTTC TGCAGGAGAA GATGGATGAA GCCCGCACTT CTGTAACTGA AGCAAAGAGA 
AGCATGTTTG CTCTCACTGA GCGCGTGCAG AGCCTCCAGG GTGAGCTGAC CCAGAGTGAA 
CTGCTGAGAG AGGAGCTGGA GGCGGAGCTT AAGAACACAC AGGAGGCACT CCGACAGCGC 
TCAGGAAGTT TGGCAGAGAC TCAGCACAGC ATCCAGAAAG CTCAGACGGA GCGGGCAGCT 
GTGGAGGAGC GTCTGCTCGC ACTGCAAAGA GCTGTGGCTC TGCTGGAGAC AGAGAAAAAA 
GATGCAGAGA GGCAAGCTGC AAGGCTGGAG AAGGACAAGG ATGCACTGAG GAACACGTTG 
GATAAGGTTG AAAGGCAGAA ACTGAAGTGT GAGGAGGGCA GCATGCGGCT CTCAGCAGAG 
AAGACCCGGC TGGACCGCTC CCTGAACGCA GTGGAGCAGG AGCTGCAGGA GGCGCAGCAG 
CAGATGCTGA TGCTGCAGAC TCAGCTGGCT GAGATGGAGC AGGCCCACAG TGGGTGTGAG 
GGTTTGCTGC GACAGCGGGA TGAAGCTCAG CAGGAGGCGG AGAAACTGAG GAACAGCTGT 
AAGGACCTGG AACGCACTCT GAGCACCAGA GAACGAGCCC ACCGGCACCG AGTCAAAGGA 
CTGGAGGAGC AGGTGTCCAC TTTGAAGGAG CAGCTGCAGC AGGAGATGAA ACGGCGGCAG 
CCTCCTCTTC CGTCCACGTT TACGTCCACT GGGATCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

crocc ( XM_020701418.2 ) cDNA ORF clone, Oryzias latipes(Japanese medaka) -> XP_020557077.1 Oryzias latipes ciliary rootlet coiled-coil, rootletin (crocc), transcript variant X1, mRNA.

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CloneID	OJa25264
Clone ID Related Accession (Same CDS sequence)	XM_020701418.1 , XM_020701418.2
Accession Version	XM_020701418.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6039bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1518537600000
Organism	Oryzias latipes(Japanese medaka)
Product	rootletin isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_019863.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Feb 15, 2018 this sequence version replaced XM_020701418.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Oryzias latipes Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCGCTG CGGAAAACTC CGACAGCGAC CCCAGCAAGC TGGAGTCAGT GATCCGGAGG 
CTGGAGGAAA GTGTTCTGTC TGAGGAGAAG AGGTTGACGG TTCGAGGCCC TTCACCGGAT 
GACCCCCCTG CGTGTCTGCC TGCCAGAGTG CGAGAGATCG TCACGAAGAA CCTCAGTGAC 
AGCACAGGGG AGATGTCCTC GCTCATGTCC CTCCAGGAGG AGAACCGGGT GCTGCAGGGC 
GAGCTGGCCC GGCTGGAGGA CCTGCTGGCG CACAGCAGGG CGGATCGGGA CGAGCTCGCC 
ATCAAGTACG GGGCAATCAG TGAACGGCTG GAGCAGGCTC TACGCTCAGA AACGGGGGAG 
GGAGAGGCCG ACTCACAGGC GTCCCGCAGC CTGGCTCAGC AGAATGTGGA ATTGCGCCGA 
CGGCTGGACG AGGAACAGGC TGCATACAGG CGCAAACTCA CCGCCTACCA AGAAGGCCAG 
CAGAGGCAGG CGCAGCTGGT GCAGAAGCTT CAGGCCAAGG TGCTTCAGTA CAAAAAGAGA 
TGTGGGGATC TGGAGCAGAA GCTGCAGGAG AAGTCCTCAG AGTTAGAGAA ATGCAGTGAG 
CGCAGTGAAA CGTCTAACGG TCGCCATCAC GAGGACTCGA GCAGCAGTTT GGAGGAGGCT 
TTACTACACC TGGAGGAGGA GCAACAGAGG AGCAGCAGTT TATCTGCAGT GAACGCCATG 
CTGAGAGAAC AGTTGGAGCA GGCCGGCTTG GCCAACGAGG CTCTGAGCCG GGACATCCGG 
AGGCTCACCG CTGATTGGAC GAAAGCCAGA GAGGAGCTGG AACAAAAGGA GTCGGACTGG 
AGGAGAGAGG AGGAGTCTTT CCACAGCTAC TTCAGCAGTG AACACGGTCG ACTGCTGACC 
TTTTGGCGAC AGGTGGTGGG GTTCAGGAGG CACGTCTGTG AGATGAAGAG CAGCGCTGAG 
AGGGACCTGT CAGACATGCG GGGCGAGCTG GTTCGGATGT CCCACTTGGT CCAGGTGTCC 
TGCGCGGGTC TGTCCGCCGT GCTGCGCAGT CAGGAAGCAG GAACGGCTCT AGCCCTGGAG 
CAGGAGAGAG CTCTGAGGGA TCAGCTGGAG CAGCAGCTGA GAGGTCAAGT CAAAGAGACG 
ATGAACCTTC AGAGCAGAGC CGATGCAGAG AGAAGTGAGC TGAGCCTCAG GCTTTCAGAT 
GCGGCTCAAG AAAATGAAAG ACTAAAAAGT CAAATGGAAG AAAGAGACCG TGAGATCGAC 
AATCTGAGCA GGAGGCTTGA GGAGCAGAGC CGCAGCGATG AGACGGACTC CCAGCTGCTG 
AGGGCTCATT GCAACGCTCT GTTGGACACG CTGAAGGACA TCGCTCAGAC GGTTCTGTCA 
GACGGAGAGT CGTCTTCAGA GGCCGATCCG GACAACTCGG CGGTCGTGCT GCAGGCGCTG 
ATCCGTGACT CCTCCCCTCA ACGCTCCTCC CAAACCAGCT CCTCGTTGTC CCCTCTCCCG 
GAAGCGGCGC TGTCTGCGCT GCGTTCGGTG GTCACAAACA TGATGCTGCA GCTGCAGGAT 
GTTCGAGGTC GTCTGCTCTC TGCACAGTCC TCTGCGCAGA AGTTTCGCAA GCAGCTGTCA 
GAAATCGACT CTGCTAAAAG AGACGCAGAC CAACGAAACC AAGCTCTGCA GCGAGACAAG 
GACGCTGCTC TCAGAGAGCG GGAAACTGCC CAGAGGGAGA AGGAGCAACT GAAGCAAGAG 
AGAGACAAGC TGGCCAGTGA GAAGGCCAAC CTGGAGAAGG CTGTTCAGGC GCTGCACAGC 
AGCAACCAGA TGCTGCAGAT GGACTACGAG AAGCTCCAGC TGGCTGTGGC TTCTGCACAG 
CGAGAGCGAG ACCGCGAGAG GGAGGAGAAG GAGGCTGCCA TCCATGACGG GGAGCGCTGG 
AAGACGGAGA TTCAGAGGAT ACAGAAGCAG AGGGACCAGA GTGAAAGTCG AGCCTCGGCT 
CAGCGGGGAG AACTGTCTGC AGTGTTACAA ACTCACCAGC AGGGGGAGGT GGAGAGGCAG 
CTGCTGGAAC AGGAGAGTGC CCACCTTTCA GAGGCCCTTG CTCGGGCTGC AAGCACCAAC 
AAAGAGCTGT CGCTGCATCT GAGCAAGCTG CAGTATGAAG ATGCAGCTCT CAGAGACTCT 
CTCACAAAGA TGGGAAACCT GAATGAGGGT CTGGTCCAGG ACAAGGTGGA CCTTAACAAG 
TATATTCTGC AGCTTGAGGA GGAGAAGGCC GTCCTACAGG CTCAGAAACG GGAGGCGGAG 
CAGGAGAAAC TGTCCATCAG GGACGAGCTG GTCCGGCAGG AGCAGAACCG ACTGGAGTTG 
GAGTCTTCCC GCGTCACACT GCAGCAGTCT CTGCAGGACA CCGAGTTCAG TCGGGTGGGC 
GTGGAGGCAG AGCTGCAGAA CCTCAGGGCT GAACGACTGA AGCTACAGGA AAAGGTCGCC 
CAGTTGTGTG GTGAGGTGGC CTCTCTAGAT TCAGAGTTGA GTCTCGCCAA GGGGGAGGGC 
CAGAGGAGCG AAATGGCCTT GGAGGAAGCC GGCCGCAGTC GAGCAGAACT GACCAAAGAC 
AAAGCAGCTC TAGTGGTTCA GCTGACGGCA TCTGAGAGAG AAAACGCAAT GCTAGCCGAG 
GAACTGACAG CTTTCAGGTC GGAGCGGGAG ACCCTGGAAA CGAGTCTCTT TGATGCGCAG 
CAGCAGCTTT TGCAGGCAGA GTCTTGCAGG GAACAGCTGG AAGCAGAAAA CCAAAACCTC 
CGCGTTCGCT GTGAAACTGC CACAGTGGAG CTCAGGCGTT TGCGCTCAGA TGCTGAGAAT 
CAGCTGGCCC AGGCTGAGAG AGACAAACAA GCGCTGACTC AAGCTCTCAG CGCCGCTCAG 
CTGGAGGCCC AGCAGGCTTC ACGCAAGGCC ACCTCCGAGC ATCAGGAGGA GGTGGAGAGA 
CTGGCCTTAG AGAAGGAAGT CGTGCGGCAC AGCTTGCAGA TGGAGCACGA GACGGCTGTC 
CGGAAACTCC GACAAGAAGT CGAAGATCAG CTCCAGAAAG CCAGGAGAGA AAAAGAGGAG 
CTGCAGGACG GACTGAGGGC TCTGCAGCAC GAGCGAGATC AGAGCCTGCT GCAGGCGGAA 
ACGGAGAAAC AACAGGCCCT TTCACTGAAG GAGGCAGAGA AAACGGTTTT GTTGGAGAAG 
GTGTCCGGTC TGCAGGCTGA TCTGTCAGCG CTGAGCCTGG AGGCTGAGCG GACAGCCAGA 
GAAGCTGCCC AGTACAAAGA GCAGGAGCAG ATCAGAGTTG GGGCTCTAAC CAGCGAACTG 
CAAGAACTTC GGTCACAGAT GGAAGATGCA GCGGCCGGTC ATGAAAGGGA CATCCTGAGT 
CTGAACGAAA CCTGTGCAGA CCTCCGTTCT CGTGCTGATG TTGCCCTCAA AGAGCTGGAG 
CAGTGCAGAG CTTCTCTCTC TGCTGCTGAG GAGAATCGAG AGCAGCTGAG GCGGGACTTC 
CTGGAGACGG AGCGCAGACT CACCCAAACT CAGGAAGCGT CAGAAAACCA CCGGAGGGAA 
GGAGCCGAGC TGCGGCGCAG CCTCTGTGAT GTCACCAGGG AGAGAGATGC TCTCAGCCAA 
TCAAATGCTC AGCTGAGGGA AGCTCTCCGA AGCGCAGAAA CGGAGAGAAT AAGCGTGAAA 
CGGCAGTGTG AGGAGAAGGA GCAGCGGCTG GTTGTGCTGG AGGAGAGCCT GTCCTCCTCG 
CAGAAGGAGG TGGCAGAGCT GCGGAGTTGC CTGAGGGAGG TGGAAAGGTC CCGCCTTGAG 
GCTCGCCGTG AGCTTCAGGA GCTTCGGAGA CAGCTGAAGG TTCTGGATGC AGACAGAGAG 
CAGAAAGGGA GGGAGCTGGC GGAGCTGCAA ACCCGCTTGT CGTTGGAGGA GCAAAGAGAG 
GAGGAAAGAG GAAAGGAGGT TTTCATTTTA AAACAGAAGC TCGCTGAAAC CCAAACGGCC 
AGAGAGTCCC TCAAGAGAGA GCTCTCCATG ACACAGAAGC GTCTGGTGGA GTCCGAGTCC 
GGCTGGCGCA GCTGTGAGCG GGAGCTGACG GCACAGCTGC AGGAGGCGCG AGGCTGCGAG 
AAGAAGCTCC AGGATGAGGC CAAGAACCTG TCGGTGCGCG CGCAAGCGGC GCAGGAATCC 
GCCGCTCAGT GCAGCCTGCA GCTCAGCGAG GCGCAGGGGA GACTGACCGC CACCGAAGCT 
GAGCTGACGC GGGCGGAGGC CTCCAAGAGG GATCTGGAGT TTCGCCTCAG CAGCCTCCAG 
TCGGCGCTGA CGCGAACGCT GGGCATCGGG GCAAGCGGCA GGGCGAGCAG GGGGAGGAGT 
CCAGGCGGGA GCTCTGCATC ACCTGGCAGT ATCTCCCGTC ACCACAGCAT CTCACCTTTG 
CGCCCTTCGC TGTCGCCCCC TAAAGAATTC AGAGGAAGCA CGCCTGATAA CACCATGGGA 
TCAGGAGCTG TGTCTCCTGA AGGAGGCGGT ACTCCTTTGC CTCCCGCTCA GCCAGAGCTG 
GATCCCGACA CGCTGCGCAG TGGCCTCAGA GACTTCCTGC AGGAGCTGCG AGACGCACAC 
AGACAAAGGG ATGATGCCCA ATGCCAGCTG ATGACTCTGC AGCGGGAGCT GGAGGAGCAG 
AAGCTGGAGC GAGAATGTGC CGAGAACCGC TCCACTCAGC TGCAAAACAG CCTGAAGGAA 
TGCCAAGAAG GGAAGCGTGG ACTTGACGAC CGTCTGACCA GCACTCAGAT TCTCCTGCAG 
CAGCAGGAGG ATGCGGTGAA GAGAGTGGAC AGAGAGAGGA GAGCTCTTGC CGACAGGGTG 
AGGGAGTTGG AGCGCTCGCT GCACACCGCA GAGATGGAGA AGAAGCACAC TCAGGAGCAG 
TTGAATAAGC AGCGGGCCAC TGAGGCACGC CTGGAGGCGG AGAGGAAGCG CCTGCGGGAG 
GCTCTGGAGG CCGCCGAAGC TCGAGCCACC CGGGTGGAGC TGGGGAGGCG CAGCCTGGAA 
GGGGAGCTGC AGAGGCTCAA ACTCGGTCTG GGAGACCGGG AGGCTGAAAG CCAGGCCTCA 
CAGGAGCGCC ACGACTGTTT GCTCAGACAG GTGGCGGAAG GAGAAGCACA AGTCTCTCTG 
CTGCAGACAG AGGTGGACAG ACTGAGTCAA GCTCTGCTGA AAGCTCAGGA AGGGGAAGCT 
GCTCTGAAGC AGAAGGTCAC GACTCTCAGC CAGAGCCTCC AGGAGGCTGC AGCTGATCAC 
AGCAGCACGC AGACTCGCCT CACTGCTCTG CAGAAAACAC TGAGCTTGGT GGAACAGGAC 
AAAAGACTTC TGCAGGAGAA GATGGATGAA GCCCGCACTT CTGTAACTGA AGCAAAGAGA 
AGCATGTTTG CTCTCACTGA GCGCGTGCAG AGCCTCCAGG GTGAGCTGAC CCAGAGTGAA 
CTGCTGAGAG AGGAGCTGGA GGCGGAGCTT AAGAACACAC AGGAGGCACT CCGACAGCGC 
TCAGGAAGTT TGGCAGAGAC TCAGCACAGC ATCCAGAAAG CTCAGACGGA GCGGGCAGCT 
GTGGAGGAGC GTCTGCTCGC ACTGCAAAGA GCTGTGGCTC TGCTGGAGAC AGAGAAAAAA 
GATGCAGAGA GGCAAGCTGC AAGGCTGGAG AAGGACAAGG ATGCACTGAG GAACACGTTG 
GATAAGGTTG AAAGGCAGAA ACTGAAGTGT GAGGAGGGCA GCATGCGGCT CTCAGCAGAG 
AAGACCCGGC TGGACCGCTC CCTGAACGCA GTGGAGCAGG AGCTGCAGGA GGCGCAGCAG 
CAGATGCTGA TGCTGCAGAC TCAGCTGGCT GAGATGGAGC AGGCCCACAG TGGGTGTGAG 
GGTTTGCTGC GACAGCGGGA TGAAGCTCAG CAGGAGGCGG AGAAACTGAG GAACAGCTGT 
AAGGACCTGG AACGCACTCT GAGCACCAGA GAACGAGCCC ACCGGCACCG AGTCAAAGGA 
CTGGAGGAGC AGGTGTCCAC TTTGAAGGAG CAGCTGCAGC AGGAGATGAA ACGGCGGCAG 
CCTCCTCTTC CGTCCACGTT TACGTCCACT GGGATCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_020557077.1
CDS	199..6237
Translation	MSAAENSDSDPSKLESVIRRLEESVLSEEKRLTVRGPSPDDPPACLPARVREIVTKNLSDSTGEMSSLMSLQEENRVLQGELARLEDLLAHSRADRDELAIKYGAISERLEQALRSETGEGEADSQASRSLAQQNVELRRRLDEEQAAYRRKLTAYQEGQQRQAQLVQKLQAKVLQYKKRCGDLEQKLQEKSSELEKCSERSETSNGRHHEDSSSSLEEALLHLEEEQQRSSSLSAVNAMLREQLEQAGLANEALSRDIRRLTADWTKAREELEQKESDWRREEESFHSYFSSEHGRLLTFWRQVVGFRRHVCEMKSSAERDLSDMRGELVRMSHLVQVSCAGLSAVLRSQEAGTALALEQERALRDQLEQQLRGQVKETMNLQSRADAERSELSLRLSDAAQENERLKSQMEERDREIDNLSRRLEEQSRSDETDSQLLRAHCNALLDTLKDIAQTVLSDGESSSEADPDNSAVVLQALIRDSSPQRSSQTSSSLSPLPEAALSALRSVVTNMMLQLQDVRGRLLSAQSSAQKFRKQLSEIDSAKRDADQRNQALQRDKDAALRERETAQREKEQLKQERDKLASEKANLEKAVQALHSSNQMLQMDYEKLQLAVASAQRERDREREEKEAAIHDGERWKTEIQRIQKQRDQSESRASAQRGELSAVLQTHQQGEVERQLLEQESAHLSEALARAASTNKELSLHLSKLQYEDAALRDSLTKMGNLNEGLVQDKVDLNKYILQLEEEKAVLQAQKREAEQEKLSIRDELVRQEQNRLELESSRVTLQQSLQDTEFSRVGVEAELQNLRAERLKLQEKVAQLCGEVASLDSELSLAKGEGQRSEMALEEAGRSRAELTKDKAALVVQLTASERENAMLAEELTAFRSERETLETSLFDAQQQLLQAESCREQLEAENQNLRVRCETATVELRRLRSDAENQLAQAERDKQALTQALSAAQLEAQQASRKATSEHQEEVERLALEKEVVRHSLQMEHETAVRKLRQEVEDQLQKARREKEELQDGLRALQHERDQSLLQAETEKQQALSLKEAEKTVLLEKVSGLQADLSALSLEAERTAREAAQYKEQEQIRVGALTSELQELRSQMEDAAAGHERDILSLNETCADLRSRADVALKELEQCRASLSAAEENREQLRRDFLETERRLTQTQEASENHRREGAELRRSLCDVTRERDALSQSNAQLREALRSAETERISVKRQCEEKEQRLVVLEESLSSSQKEVAELRSCLREVERSRLEARRELQELRRQLKVLDADREQKGRELAELQTRLSLEEQREEERGKEVFILKQKLAETQTARESLKRELSMTQKRLVESESGWRSCERELTAQLQEARGCEKKLQDEAKNLSVRAQAAQESAAQCSLQLSEAQGRLTATEAELTRAEASKRDLEFRLSSLQSALTRTLGIGASGRASRGRSPGGSSASPGSISRHHSISPLRPSLSPPKEFRGSTPDNTMGSGAVSPEGGGTPLPPAQPELDPDTLRSGLRDFLQELRDAHRQRDDAQCQLMTLQRELEEQKLERECAENRSTQLQNSLKECQEGKRGLDDRLTSTQILLQQQEDAVKRVDRERRALADRVRELERSLHTAEMEKKHTQEQLNKQRATEARLEAERKRLREALEAAEARATRVELGRRSLEGELQRLKLGLGDREAESQASQERHDCLLRQVAEGEAQVSLLQTEVDRLSQALLKAQEGEAALKQKVTTLSQSLQEAAADHSSTQTRLTALQKTLSLVEQDKRLLQEKMDEARTSVTEAKRSMFALTERVQSLQGELTQSELLREELEAELKNTQEALRQRSGSLAETQHSIQKAQTERAAVEERLLALQRAVALLETEKKDAERQAARLEKDKDALRNTLDKVERQKLKCEEGSMRLSAEKTRLDRSLNAVEQELQEAQQQMLMLQTQLAEMEQAHSGCEGLLRQRDEAQQEAEKLRNSCKDLERTLSTRERAHRHRVKGLEEQVSTLKEQLQQEMKRRQPPLPSTFTSTGI

Target ORF information:

RefSeq Version	XM_020701418.2
Organism	Oryzias latipes(Japanese medaka)
Definition	Oryzias latipes ciliary rootlet coiled-coil, rootletin (crocc), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_020701418.2

ORF Insert Sequence:

ATGAGCGCTG CGGAAAACTC CGACAGCGAC CCCAGCAAGC TGGAGTCAGT GATCCGGAGG 
CTGGAGGAAA GTGTTCTGTC TGAGGAGAAG AGGTTGACGG TTCGAGGCCC TTCACCGGAT 
GACCCCCCTG CGTGTCTGCC TGCCAGAGTG CGAGAGATCG TCACGAAGAA CCTCAGTGAC 
AGCACAGGGG AGATGTCCTC GCTCATGTCC CTCCAGGAGG AGAACCGGGT GCTGCAGGGC 
GAGCTGGCCC GGCTGGAGGA CCTGCTGGCG CACAGCAGGG CGGATCGGGA CGAGCTCGCC 
ATCAAGTACG GGGCAATCAG TGAACGGCTG GAGCAGGCTC TACGCTCAGA AACGGGGGAG 
GGAGAGGCCG ACTCACAGGC GTCCCGCAGC CTGGCTCAGC AGAATGTGGA ATTGCGCCGA 
CGGCTGGACG AGGAACAGGC TGCATACAGG CGCAAACTCA CCGCCTACCA AGAAGGCCAG 
CAGAGGCAGG CGCAGCTGGT GCAGAAGCTT CAGGCCAAGG TGCTTCAGTA CAAAAAGAGA 
TGTGGGGATC TGGAGCAGAA GCTGCAGGAG AAGTCCTCAG AGTTAGAGAA ATGCAGTGAG 
CGCAGTGAAA CGTCTAACGG TCGCCATCAC GAGGACTCGA GCAGCAGTTT GGAGGAGGCT 
TTACTACACC TGGAGGAGGA GCAACAGAGG AGCAGCAGTT TATCTGCAGT GAACGCCATG 
CTGAGAGAAC AGTTGGAGCA GGCCGGCTTG GCCAACGAGG CTCTGAGCCG GGACATCCGG 
AGGCTCACCG CTGATTGGAC GAAAGCCAGA GAGGAGCTGG AACAAAAGGA GTCGGACTGG 
AGGAGAGAGG AGGAGTCTTT CCACAGCTAC TTCAGCAGTG AACACGGTCG ACTGCTGACC 
TTTTGGCGAC AGGTGGTGGG GTTCAGGAGG CACGTCTGTG AGATGAAGAG CAGCGCTGAG 
AGGGACCTGT CAGACATGCG GGGCGAGCTG GTTCGGATGT CCCACTTGGT CCAGGTGTCC 
TGCGCGGGTC TGTCCGCCGT GCTGCGCAGT CAGGAAGCAG GAACGGCTCT AGCCCTGGAG 
CAGGAGAGAG CTCTGAGGGA TCAGCTGGAG CAGCAGCTGA GAGGTCAAGT CAAAGAGACG 
ATGAACCTTC AGAGCAGAGC CGATGCAGAG AGAAGTGAGC TGAGCCTCAG GCTTTCAGAT 
GCGGCTCAAG AAAATGAAAG ACTAAAAAGT CAAATGGAAG AAAGAGACCG TGAGATCGAC 
AATCTGAGCA GGAGGCTTGA GGAGCAGAGC CGCAGCGATG AGACGGACTC CCAGCTGCTG 
AGGGCTCATT GCAACGCTCT GTTGGACACG CTGAAGGACA TCGCTCAGAC GGTTCTGTCA 
GACGGAGAGT CGTCTTCAGA GGCCGATCCG GACAACTCGG CGGTCGTGCT GCAGGCGCTG 
ATCCGTGACT CCTCCCCTCA ACGCTCCTCC CAAACCAGCT CCTCGTTGTC CCCTCTCCCG 
GAAGCGGCGC TGTCTGCGCT GCGTTCGGTG GTCACAAACA TGATGCTGCA GCTGCAGGAT 
GTTCGAGGTC GTCTGCTCTC TGCACAGTCC TCTGCGCAGA AGTTTCGCAA GCAGCTGTCA 
GAAATCGACT CTGCTAAAAG AGACGCAGAC CAACGAAACC AAGCTCTGCA GCGAGACAAG 
GACGCTGCTC TCAGAGAGCG GGAAACTGCC CAGAGGGAGA AGGAGCAACT GAAGCAAGAG 
AGAGACAAGC TGGCCAGTGA GAAGGCCAAC CTGGAGAAGG CTGTTCAGGC GCTGCACAGC 
AGCAACCAGA TGCTGCAGAT GGACTACGAG AAGCTCCAGC TGGCTGTGGC TTCTGCACAG 
CGAGAGCGAG ACCGCGAGAG GGAGGAGAAG GAGGCTGCCA TCCATGACGG GGAGCGCTGG 
AAGACGGAGA TTCAGAGGAT ACAGAAGCAG AGGGACCAGA GTGAAAGTCG AGCCTCGGCT 
CAGCGGGGAG AACTGTCTGC AGTGTTACAA ACTCACCAGC AGGGGGAGGT GGAGAGGCAG 
CTGCTGGAAC AGGAGAGTGC CCACCTTTCA GAGGCCCTTG CTCGGGCTGC AAGCACCAAC 
AAAGAGCTGT CGCTGCATCT GAGCAAGCTG CAGTATGAAG ATGCAGCTCT CAGAGACTCT 
CTCACAAAGA TGGGAAACCT GAATGAGGGT CTGGTCCAGG ACAAGGTGGA CCTTAACAAG 
TATATTCTGC AGCTTGAGGA GGAGAAGGCC GTCCTACAGG CTCAGAAACG GGAGGCGGAG 
CAGGAGAAAC TGTCCATCAG GGACGAGCTG GTCCGGCAGG AGCAGAACCG ACTGGAGTTG 
GAGTCTTCCC GCGTCACACT GCAGCAGTCT CTGCAGGACA CCGAGTTCAG TCGGGTGGGC 
GTGGAGGCAG AGCTGCAGAA CCTCAGGGCT GAACGACTGA AGCTACAGGA AAAGGTCGCC 
CAGTTGTGTG GTGAGGTGGC CTCTCTAGAT TCAGAGTTGA GTCTCGCCAA GGGGGAGGGC 
CAGAGGAGCG AAATGGCCTT GGAGGAAGCC GGCCGCAGTC GAGCAGAACT GACCAAAGAC 
AAAGCAGCTC TAGTGGTTCA GCTGACGGCA TCTGAGAGAG AAAACGCAAT GCTAGCCGAG 
GAACTGACAG CTTTCAGGTC GGAGCGGGAG ACCCTGGAAA CGAGTCTCTT TGATGCGCAG 
CAGCAGCTTT TGCAGGCAGA GTCTTGCAGG GAACAGCTGG AAGCAGAAAA CCAAAACCTC 
CGCGTTCGCT GTGAAACTGC CACAGTGGAG CTCAGGCGTT TGCGCTCAGA TGCTGAGAAT 
CAGCTGGCCC AGGCTGAGAG AGACAAACAA GCGCTGACTC AAGCTCTCAG CGCCGCTCAG 
CTGGAGGCCC AGCAGGCTTC ACGCAAGGCC ACCTCCGAGC ATCAGGAGGA GGTGGAGAGA 
CTGGCCTTAG AGAAGGAAGT CGTGCGGCAC AGCTTGCAGA TGGAGCACGA GACGGCTGTC 
CGGAAACTCC GACAAGAAGT CGAAGATCAG CTCCAGAAAG CCAGGAGAGA AAAAGAGGAG 
CTGCAGGACG GACTGAGGGC TCTGCAGCAC GAGCGAGATC AGAGCCTGCT GCAGGCGGAA 
ACGGAGAAAC AACAGGCCCT TTCACTGAAG GAGGCAGAGA AAACGGTTTT GTTGGAGAAG 
GTGTCCGGTC TGCAGGCTGA TCTGTCAGCG CTGAGCCTGG AGGCTGAGCG GACAGCCAGA 
GAAGCTGCCC AGTACAAAGA GCAGGAGCAG ATCAGAGTTG GGGCTCTAAC CAGCGAACTG 
CAAGAACTTC GGTCACAGAT GGAAGATGCA GCGGCCGGTC ATGAAAGGGA CATCCTGAGT 
CTGAACGAAA CCTGTGCAGA CCTCCGTTCT CGTGCTGATG TTGCCCTCAA AGAGCTGGAG 
CAGTGCAGAG CTTCTCTCTC TGCTGCTGAG GAGAATCGAG AGCAGCTGAG GCGGGACTTC 
CTGGAGACGG AGCGCAGACT CACCCAAACT CAGGAAGCGT CAGAAAACCA CCGGAGGGAA 
GGAGCCGAGC TGCGGCGCAG CCTCTGTGAT GTCACCAGGG AGAGAGATGC TCTCAGCCAA 
TCAAATGCTC AGCTGAGGGA AGCTCTCCGA AGCGCAGAAA CGGAGAGAAT AAGCGTGAAA 
CGGCAGTGTG AGGAGAAGGA GCAGCGGCTG GTTGTGCTGG AGGAGAGCCT GTCCTCCTCG 
CAGAAGGAGG TGGCAGAGCT GCGGAGTTGC CTGAGGGAGG TGGAAAGGTC CCGCCTTGAG 
GCTCGCCGTG AGCTTCAGGA GCTTCGGAGA CAGCTGAAGG TTCTGGATGC AGACAGAGAG 
CAGAAAGGGA GGGAGCTGGC GGAGCTGCAA ACCCGCTTGT CGTTGGAGGA GCAAAGAGAG 
GAGGAAAGAG GAAAGGAGGT TTTCATTTTA AAACAGAAGC TCGCTGAAAC CCAAACGGCC 
AGAGAGTCCC TCAAGAGAGA GCTCTCCATG ACACAGAAGC GTCTGGTGGA GTCCGAGTCC 
GGCTGGCGCA GCTGTGAGCG GGAGCTGACG GCACAGCTGC AGGAGGCGCG AGGCTGCGAG 
AAGAAGCTCC AGGATGAGGC CAAGAACCTG TCGGTGCGCG CGCAAGCGGC GCAGGAATCC 
GCCGCTCAGT GCAGCCTGCA GCTCAGCGAG GCGCAGGGGA GACTGACCGC CACCGAAGCT 
GAGCTGACGC GGGCGGAGGC CTCCAAGAGG GATCTGGAGT TTCGCCTCAG CAGCCTCCAG 
TCGGCGCTGA CGCGAACGCT GGGCATCGGG GCAAGCGGCA GGGCGAGCAG GGGGAGGAGT 
CCAGGCGGGA GCTCTGCATC ACCTGGCAGT ATCTCCCGTC ACCACAGCAT CTCACCTTTG 
CGCCCTTCGC TGTCGCCCCC TAAAGAATTC AGAGGAAGCA CGCCTGATAA CACCATGGGA 
TCAGGAGCTG TGTCTCCTGA AGGAGGCGGT ACTCCTTTGC CTCCCGCTCA GCCAGAGCTG 
GATCCCGACA CGCTGCGCAG TGGCCTCAGA GACTTCCTGC AGGAGCTGCG AGACGCACAC 
AGACAAAGGG ATGATGCCCA ATGCCAGCTG ATGACTCTGC AGCGGGAGCT GGAGGAGCAG 
AAGCTGGAGC GAGAATGTGC CGAGAACCGC TCCACTCAGC TGCAAAACAG CCTGAAGGAA 
TGCCAAGAAG GGAAGCGTGG ACTTGACGAC CGTCTGACCA GCACTCAGAT TCTCCTGCAG 
CAGCAGGAGG ATGCGGTGAA GAGAGTGGAC AGAGAGAGGA GAGCTCTTGC CGACAGGGTG 
AGGGAGTTGG AGCGCTCGCT GCACACCGCA GAGATGGAGA AGAAGCACAC TCAGGAGCAG 
TTGAATAAGC AGCGGGCCAC TGAGGCACGC CTGGAGGCGG AGAGGAAGCG CCTGCGGGAG 
GCTCTGGAGG CCGCCGAAGC TCGAGCCACC CGGGTGGAGC TGGGGAGGCG CAGCCTGGAA 
GGGGAGCTGC AGAGGCTCAA ACTCGGTCTG GGAGACCGGG AGGCTGAAAG CCAGGCCTCA 
CAGGAGCGCC ACGACTGTTT GCTCAGACAG GTGGCGGAAG GAGAAGCACA AGTCTCTCTG 
CTGCAGACAG AGGTGGACAG ACTGAGTCAA GCTCTGCTGA AAGCTCAGGA AGGGGAAGCT 
GCTCTGAAGC AGAAGGTCAC GACTCTCAGC CAGAGCCTCC AGGAGGCTGC AGCTGATCAC 
AGCAGCACGC AGACTCGCCT CACTGCTCTG CAGAAAACAC TGAGCTTGGT GGAACAGGAC 
AAAAGACTTC TGCAGGAGAA GATGGATGAA GCCCGCACTT CTGTAACTGA AGCAAAGAGA 
AGCATGTTTG CTCTCACTGA GCGCGTGCAG AGCCTCCAGG GTGAGCTGAC CCAGAGTGAA 
CTGCTGAGAG AGGAGCTGGA GGCGGAGCTT AAGAACACAC AGGAGGCACT CCGACAGCGC 
TCAGGAAGTT TGGCAGAGAC TCAGCACAGC ATCCAGAAAG CTCAGACGGA GCGGGCAGCT 
GTGGAGGAGC GTCTGCTCGC ACTGCAAAGA GCTGTGGCTC TGCTGGAGAC AGAGAAAAAA 
GATGCAGAGA GGCAAGCTGC AAGGCTGGAG AAGGACAAGG ATGCACTGAG GAACACGTTG 
GATAAGGTTG AAAGGCAGAA ACTGAAGTGT GAGGAGGGCA GCATGCGGCT CTCAGCAGAG 
AAGACCCGGC TGGACCGCTC CCTGAACGCA GTGGAGCAGG AGCTGCAGGA GGCGCAGCAG 
CAGATGCTGA TGCTGCAGAC TCAGCTGGCT GAGATGGAGC AGGCCCACAG TGGGTGTGAG 
GGTTTGCTGC GACAGCGGGA TGAAGCTCAG CAGGAGGCGG AGAAACTGAG GAACAGCTGT 
AAGGACCTGG AACGCACTCT GAGCACCAGA GAACGAGCCC ACCGGCACCG AGTCAAAGGA 
CTGGAGGAGC AGGTGTCCAC TTTGAAGGAG CAGCTGCAGC AGGAGATGAA ACGGCGGCAG 
CCTCCTCTTC CGTCCACGTT TACGTCCACT GGGATCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

crocc ( XM_020701419.2 ) cDNA ORF clone, Oryzias latipes(Japanese medaka) -> XP_020557078.1 Oryzias latipes ciliary rootlet coiled-coil, rootletin (crocc), transcript variant X2, mRNA.

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CloneID	OJa25265
Clone ID Related Accession (Same CDS sequence)	XM_020701419.2 , XM_020701419.1
Accession Version	XM_020701419.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6036bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1518537600000
Organism	Oryzias latipes(Japanese medaka)
Product	rootletin isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_019863.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Feb 15, 2018 this sequence version replaced XM_020701419.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Oryzias latipes Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCGCTG CGGAAAACTC CGACAGCGAC CCCAGCAAGC TGGAGTCAGT GATCCGGAGG 
CTGGAGGAAA GTGTTCTGTC TGAGGAGAAG AGGTTGACGG TTCGAGGCCC TTCACCGGAT 
GACCCCCCTG CGTGTCTGCC TGCCAGAGTG CGAGAGATCG TCACGAAGAA CCTCAGTGAC 
AGCAGGGAGA TGTCCTCGCT CATGTCCCTC CAGGAGGAGA ACCGGGTGCT GCAGGGCGAG 
CTGGCCCGGC TGGAGGACCT GCTGGCGCAC AGCAGGGCGG ATCGGGACGA GCTCGCCATC 
AAGTACGGGG CAATCAGTGA ACGGCTGGAG CAGGCTCTAC GCTCAGAAAC GGGGGAGGGA 
GAGGCCGACT CACAGGCGTC CCGCAGCCTG GCTCAGCAGA ATGTGGAATT GCGCCGACGG 
CTGGACGAGG AACAGGCTGC ATACAGGCGC AAACTCACCG CCTACCAAGA AGGCCAGCAG 
AGGCAGGCGC AGCTGGTGCA GAAGCTTCAG GCCAAGGTGC TTCAGTACAA AAAGAGATGT 
GGGGATCTGG AGCAGAAGCT GCAGGAGAAG TCCTCAGAGT TAGAGAAATG CAGTGAGCGC 
AGTGAAACGT CTAACGGTCG CCATCACGAG GACTCGAGCA GCAGTTTGGA GGAGGCTTTA 
CTACACCTGG AGGAGGAGCA ACAGAGGAGC AGCAGTTTAT CTGCAGTGAA CGCCATGCTG 
AGAGAACAGT TGGAGCAGGC CGGCTTGGCC AACGAGGCTC TGAGCCGGGA CATCCGGAGG 
CTCACCGCTG ATTGGACGAA AGCCAGAGAG GAGCTGGAAC AAAAGGAGTC GGACTGGAGG 
AGAGAGGAGG AGTCTTTCCA CAGCTACTTC AGCAGTGAAC ACGGTCGACT GCTGACCTTT 
TGGCGACAGG TGGTGGGGTT CAGGAGGCAC GTCTGTGAGA TGAAGAGCAG CGCTGAGAGG 
GACCTGTCAG ACATGCGGGG CGAGCTGGTT CGGATGTCCC ACTTGGTCCA GGTGTCCTGC 
GCGGGTCTGT CCGCCGTGCT GCGCAGTCAG GAAGCAGGAA CGGCTCTAGC CCTGGAGCAG 
GAGAGAGCTC TGAGGGATCA GCTGGAGCAG CAGCTGAGAG GTCAAGTCAA AGAGACGATG 
AACCTTCAGA GCAGAGCCGA TGCAGAGAGA AGTGAGCTGA GCCTCAGGCT TTCAGATGCG 
GCTCAAGAAA ATGAAAGACT AAAAAGTCAA ATGGAAGAAA GAGACCGTGA GATCGACAAT 
CTGAGCAGGA GGCTTGAGGA GCAGAGCCGC AGCGATGAGA CGGACTCCCA GCTGCTGAGG 
GCTCATTGCA ACGCTCTGTT GGACACGCTG AAGGACATCG CTCAGACGGT TCTGTCAGAC 
GGAGAGTCGT CTTCAGAGGC CGATCCGGAC AACTCGGCGG TCGTGCTGCA GGCGCTGATC 
CGTGACTCCT CCCCTCAACG CTCCTCCCAA ACCAGCTCCT CGTTGTCCCC TCTCCCGGAA 
GCGGCGCTGT CTGCGCTGCG TTCGGTGGTC ACAAACATGA TGCTGCAGCT GCAGGATGTT 
CGAGGTCGTC TGCTCTCTGC ACAGTCCTCT GCGCAGAAGT TTCGCAAGCA GCTGTCAGAA 
ATCGACTCTG CTAAAAGAGA CGCAGACCAA CGAAACCAAG CTCTGCAGCG AGACAAGGAC 
GCTGCTCTCA GAGAGCGGGA AACTGCCCAG AGGGAGAAGG AGCAACTGAA GCAAGAGAGA 
GACAAGCTGG CCAGTGAGAA GGCCAACCTG GAGAAGGCTG TTCAGGCGCT GCACAGCAGC 
AACCAGATGC TGCAGATGGA CTACGAGAAG CTCCAGCTGG CTGTGGCTTC TGCACAGCGA 
GAGCGAGACC GCGAGAGGGA GGAGAAGGAG GCTGCCATCC ATGACGGGGA GCGCTGGAAG 
ACGGAGATTC AGAGGATACA GAAGCAGAGG GACCAGAGTG AAAGTCGAGC CTCGGCTCAG 
CGGGGAGAAC TGTCTGCAGT GTTACAAACT CACCAGCAGG GGGAGGTGGA GAGGCAGCTG 
CTGGAACAGG AGAGTGCCCA CCTTTCAGAG GCCCTTGCTC GGGCTGCAAG CACCAACAAA 
GAGCTGTCGC TGCATCTGAG CAAGCTGCAG TATGAAGATG CAGCTCTCAG AGACTCTCTC 
ACAAAGATGG GAAACCTGAA TGAGGGTCTG GTCCAGGACA AGGTGGACCT TAACAAGTAT 
ATTCTGCAGC TTGAGGAGGA GAAGGCCGTC CTACAGGCTC AGAAACGGGA GGCGGAGCAG 
GAGAAACTGT CCATCAGGGA CGAGCTGGTC CGGCAGGAGC AGAACCGACT GGAGTTGGAG 
TCTTCCCGCG TCACACTGCA GCAGTCTCTG CAGGACACCG AGTTCAGTCG GGTGGGCGTG 
GAGGCAGAGC TGCAGAACCT CAGGGCTGAA CGACTGAAGC TACAGGAAAA GGTCGCCCAG 
TTGTGTGGTG AGGTGGCCTC TCTAGATTCA GAGTTGAGTC TCGCCAAGGG GGAGGGCCAG 
AGGAGCGAAA TGGCCTTGGA GGAAGCCGGC CGCAGTCGAG CAGAACTGAC CAAAGACAAA 
GCAGCTCTAG TGGTTCAGCT GACGGCATCT GAGAGAGAAA ACGCAATGCT AGCCGAGGAA 
CTGACAGCTT TCAGGTCGGA GCGGGAGACC CTGGAAACGA GTCTCTTTGA TGCGCAGCAG 
CAGCTTTTGC AGGCAGAGTC TTGCAGGGAA CAGCTGGAAG CAGAAAACCA AAACCTCCGC 
GTTCGCTGTG AAACTGCCAC AGTGGAGCTC AGGCGTTTGC GCTCAGATGC TGAGAATCAG 
CTGGCCCAGG CTGAGAGAGA CAAACAAGCG CTGACTCAAG CTCTCAGCGC CGCTCAGCTG 
GAGGCCCAGC AGGCTTCACG CAAGGCCACC TCCGAGCATC AGGAGGAGGT GGAGAGACTG 
GCCTTAGAGA AGGAAGTCGT GCGGCACAGC TTGCAGATGG AGCACGAGAC GGCTGTCCGG 
AAACTCCGAC AAGAAGTCGA AGATCAGCTC CAGAAAGCCA GGAGAGAAAA AGAGGAGCTG 
CAGGACGGAC TGAGGGCTCT GCAGCACGAG CGAGATCAGA GCCTGCTGCA GGCGGAAACG 
GAGAAACAAC AGGCCCTTTC ACTGAAGGAG GCAGAGAAAA CGGTTTTGTT GGAGAAGGTG 
TCCGGTCTGC AGGCTGATCT GTCAGCGCTG AGCCTGGAGG CTGAGCGGAC AGCCAGAGAA 
GCTGCCCAGT ACAAAGAGCA GGAGCAGATC AGAGTTGGGG CTCTAACCAG CGAACTGCAA 
GAACTTCGGT CACAGATGGA AGATGCAGCG GCCGGTCATG AAAGGGACAT CCTGAGTCTG 
AACGAAACCT GTGCAGACCT CCGTTCTCGT GCTGATGTTG CCCTCAAAGA GCTGGAGCAG 
TGCAGAGCTT CTCTCTCTGC TGCTGAGGAG AATCGAGAGC AGCTGAGGCG GGACTTCCTG 
GAGACGGAGC GCAGACTCAC CCAAACTCAG GAAGCGTCAG AAAACCACCG GAGGGAAGGA 
GCCGAGCTGC GGCGCAGCCT CTGTGATGTC ACCAGGGAGA GAGATGCTCT CAGCCAATCA 
AATGCTCAGC TGAGGGAAGC TCTCCGAAGC GCAGAAACGG AGAGAATAAG CGTGAAACGG 
CAGTGTGAGG AGAAGGAGCA GCGGCTGGTT GTGCTGGAGG AGAGCCTGTC CTCCTCGCAG 
AAGGAGGTGG CAGAGCTGCG GAGTTGCCTG AGGGAGGTGG AAAGGTCCCG CCTTGAGGCT 
CGCCGTGAGC TTCAGGAGCT TCGGAGACAG CTGAAGGTTC TGGATGCAGA CAGAGAGCAG 
AAAGGGAGGG AGCTGGCGGA GCTGCAAACC CGCTTGTCGT TGGAGGAGCA AAGAGAGGAG 
GAAAGAGGAA AGGAGGTTTT CATTTTAAAA CAGAAGCTCG CTGAAACCCA AACGGCCAGA 
GAGTCCCTCA AGAGAGAGCT CTCCATGACA CAGAAGCGTC TGGTGGAGTC CGAGTCCGGC 
TGGCGCAGCT GTGAGCGGGA GCTGACGGCA CAGCTGCAGG AGGCGCGAGG CTGCGAGAAG 
AAGCTCCAGG ATGAGGCCAA GAACCTGTCG GTGCGCGCGC AAGCGGCGCA GGAATCCGCC 
GCTCAGTGCA GCCTGCAGCT CAGCGAGGCG CAGGGGAGAC TGACCGCCAC CGAAGCTGAG 
CTGACGCGGG CGGAGGCCTC CAAGAGGGAT CTGGAGTTTC GCCTCAGCAG CCTCCAGTCG 
GCGCTGACGC GAACGCTGGG CATCGGGGCA AGCGGCAGGG CGAGCAGGGG GAGGAGTCCA 
GGCGGGAGCT CTGCATCACC TGGCAGTATC TCCCGTCACC ACAGCATCTC ACCTTTGCGC 
CCTTCGCTGT CGCCCCCTAA AGAATTCAGA GGAAGCACGC CTGATAACAC CATGGGATCA 
GGAGCTGTGT CTCCTGAAGG AGGCGGTACT CCTTTGCCTC CCGCTCAGCC AGAGCTGGAT 
CCCGACACGC TGCGCAGTGG CCTCAGAGAC TTCCTGCAGG AGCTGCGAGA CGCACACAGA 
CAAAGGGATG ATGCCCAATG CCAGCTGATG ACTCTGCAGC GGGAGCTGGA GGAGCAGAAG 
CTGGAGCGAG AATGTGCCGA GAACCGCTCC ACTCAGCTGC AAAACAGCCT GAAGGAATGC 
CAAGAAGGGA AGCGTGGACT TGACGACCGT CTGACCAGCA CTCAGATTCT CCTGCAGCAG 
CAGGAGGATG CGGTGAAGAG AGTGGACAGA GAGAGGAGAG CTCTTGCCGA CAGGGTGAGG 
GAGTTGGAGC GCTCGCTGCA CACCGCAGAG ATGGAGAAGA AGCACACTCA GGAGCAGTTG 
AATAAGCAGC GGGCCACTGA GGCACGCCTG GAGGCGGAGA GGAAGCGCCT GCGGGAGGCT 
CTGGAGGCCG CCGAAGCTCG AGCCACCCGG GTGGAGCTGG GGAGGCGCAG CCTGGAAGGG 
GAGCTGCAGA GGCTCAAACT CGGTCTGGGA GACCGGGAGG CTGAAAGCCA GGCCTCACAG 
GAGCGCCACG ACTGTTTGCT CAGACAGGTG GCGGAAGGAG AAGCACAAGT CTCTCTGCTG 
CAGACAGAGG TGGACAGACT GAGTCAAGCT CTGCTGAAAG CTCAGGAAGG GGAAGCTGCT 
CTGAAGCAGA AGGTCACGAC TCTCAGCCAG AGCCTCCAGG AGGCTGCAGC TGATCACAGC 
AGCACGCAGA CTCGCCTCAC TGCTCTGCAG AAAACACTGA GCTTGGTGGA ACAGGACAAA 
AGACTTCTGC AGGAGAAGAT GGATGAAGCC CGCACTTCTG TAACTGAAGC AAAGAGAAGC 
ATGTTTGCTC TCACTGAGCG CGTGCAGAGC CTCCAGGGTG AGCTGACCCA GAGTGAACTG 
CTGAGAGAGG AGCTGGAGGC GGAGCTTAAG AACACACAGG AGGCACTCCG ACAGCGCTCA 
GGAAGTTTGG CAGAGACTCA GCACAGCATC CAGAAAGCTC AGACGGAGCG GGCAGCTGTG 
GAGGAGCGTC TGCTCGCACT GCAAAGAGCT GTGGCTCTGC TGGAGACAGA GAAAAAAGAT 
GCAGAGAGGC AAGCTGCAAG GCTGGAGAAG GACAAGGATG CACTGAGGAA CACGTTGGAT 
AAGGTTGAAA GGCAGAAACT GAAGTGTGAG GAGGGCAGCA TGCGGCTCTC AGCAGAGAAG 
ACCCGGCTGG ACCGCTCCCT GAACGCAGTG GAGCAGGAGC TGCAGGAGGC GCAGCAGCAG 
ATGCTGATGC TGCAGACTCA GCTGGCTGAG ATGGAGCAGG CCCACAGTGG GTGTGAGGGT 
TTGCTGCGAC AGCGGGATGA AGCTCAGCAG GAGGCGGAGA AACTGAGGAA CAGCTGTAAG 
GACCTGGAAC GCACTCTGAG CACCAGAGAA CGAGCCCACC GGCACCGAGT CAAAGGACTG 
GAGGAGCAGG TGTCCACTTT GAAGGAGCAG CTGCAGCAGG AGATGAAACG GCGGCAGCCT 
CCTCTTCCGT CCACGTTTAC GTCCACTGGG ATCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_020557078.1
CDS	199..6234
Translation	MSAAENSDSDPSKLESVIRRLEESVLSEEKRLTVRGPSPDDPPACLPARVREIVTKNLSDSREMSSLMSLQEENRVLQGELARLEDLLAHSRADRDELAIKYGAISERLEQALRSETGEGEADSQASRSLAQQNVELRRRLDEEQAAYRRKLTAYQEGQQRQAQLVQKLQAKVLQYKKRCGDLEQKLQEKSSELEKCSERSETSNGRHHEDSSSSLEEALLHLEEEQQRSSSLSAVNAMLREQLEQAGLANEALSRDIRRLTADWTKAREELEQKESDWRREEESFHSYFSSEHGRLLTFWRQVVGFRRHVCEMKSSAERDLSDMRGELVRMSHLVQVSCAGLSAVLRSQEAGTALALEQERALRDQLEQQLRGQVKETMNLQSRADAERSELSLRLSDAAQENERLKSQMEERDREIDNLSRRLEEQSRSDETDSQLLRAHCNALLDTLKDIAQTVLSDGESSSEADPDNSAVVLQALIRDSSPQRSSQTSSSLSPLPEAALSALRSVVTNMMLQLQDVRGRLLSAQSSAQKFRKQLSEIDSAKRDADQRNQALQRDKDAALRERETAQREKEQLKQERDKLASEKANLEKAVQALHSSNQMLQMDYEKLQLAVASAQRERDREREEKEAAIHDGERWKTEIQRIQKQRDQSESRASAQRGELSAVLQTHQQGEVERQLLEQESAHLSEALARAASTNKELSLHLSKLQYEDAALRDSLTKMGNLNEGLVQDKVDLNKYILQLEEEKAVLQAQKREAEQEKLSIRDELVRQEQNRLELESSRVTLQQSLQDTEFSRVGVEAELQNLRAERLKLQEKVAQLCGEVASLDSELSLAKGEGQRSEMALEEAGRSRAELTKDKAALVVQLTASERENAMLAEELTAFRSERETLETSLFDAQQQLLQAESCREQLEAENQNLRVRCETATVELRRLRSDAENQLAQAERDKQALTQALSAAQLEAQQASRKATSEHQEEVERLALEKEVVRHSLQMEHETAVRKLRQEVEDQLQKARREKEELQDGLRALQHERDQSLLQAETEKQQALSLKEAEKTVLLEKVSGLQADLSALSLEAERTAREAAQYKEQEQIRVGALTSELQELRSQMEDAAAGHERDILSLNETCADLRSRADVALKELEQCRASLSAAEENREQLRRDFLETERRLTQTQEASENHRREGAELRRSLCDVTRERDALSQSNAQLREALRSAETERISVKRQCEEKEQRLVVLEESLSSSQKEVAELRSCLREVERSRLEARRELQELRRQLKVLDADREQKGRELAELQTRLSLEEQREEERGKEVFILKQKLAETQTARESLKRELSMTQKRLVESESGWRSCERELTAQLQEARGCEKKLQDEAKNLSVRAQAAQESAAQCSLQLSEAQGRLTATEAELTRAEASKRDLEFRLSSLQSALTRTLGIGASGRASRGRSPGGSSASPGSISRHHSISPLRPSLSPPKEFRGSTPDNTMGSGAVSPEGGGTPLPPAQPELDPDTLRSGLRDFLQELRDAHRQRDDAQCQLMTLQRELEEQKLERECAENRSTQLQNSLKECQEGKRGLDDRLTSTQILLQQQEDAVKRVDRERRALADRVRELERSLHTAEMEKKHTQEQLNKQRATEARLEAERKRLREALEAAEARATRVELGRRSLEGELQRLKLGLGDREAESQASQERHDCLLRQVAEGEAQVSLLQTEVDRLSQALLKAQEGEAALKQKVTTLSQSLQEAAADHSSTQTRLTALQKTLSLVEQDKRLLQEKMDEARTSVTEAKRSMFALTERVQSLQGELTQSELLREELEAELKNTQEALRQRSGSLAETQHSIQKAQTERAAVEERLLALQRAVALLETEKKDAERQAARLEKDKDALRNTLDKVERQKLKCEEGSMRLSAEKTRLDRSLNAVEQELQEAQQQMLMLQTQLAEMEQAHSGCEGLLRQRDEAQQEAEKLRNSCKDLERTLSTRERAHRHRVKGLEEQVSTLKEQLQQEMKRRQPPLPSTFTSTGI

Target ORF information:

RefSeq Version	XM_020701419.2
Organism	Oryzias latipes(Japanese medaka)
Definition	Oryzias latipes ciliary rootlet coiled-coil, rootletin (crocc), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_020701419.2

ORF Insert Sequence:

ATGAGCGCTG CGGAAAACTC CGACAGCGAC CCCAGCAAGC TGGAGTCAGT GATCCGGAGG 
CTGGAGGAAA GTGTTCTGTC TGAGGAGAAG AGGTTGACGG TTCGAGGCCC TTCACCGGAT 
GACCCCCCTG CGTGTCTGCC TGCCAGAGTG CGAGAGATCG TCACGAAGAA CCTCAGTGAC 
AGCAGGGAGA TGTCCTCGCT CATGTCCCTC CAGGAGGAGA ACCGGGTGCT GCAGGGCGAG 
CTGGCCCGGC TGGAGGACCT GCTGGCGCAC AGCAGGGCGG ATCGGGACGA GCTCGCCATC 
AAGTACGGGG CAATCAGTGA ACGGCTGGAG CAGGCTCTAC GCTCAGAAAC GGGGGAGGGA 
GAGGCCGACT CACAGGCGTC CCGCAGCCTG GCTCAGCAGA ATGTGGAATT GCGCCGACGG 
CTGGACGAGG AACAGGCTGC ATACAGGCGC AAACTCACCG CCTACCAAGA AGGCCAGCAG 
AGGCAGGCGC AGCTGGTGCA GAAGCTTCAG GCCAAGGTGC TTCAGTACAA AAAGAGATGT 
GGGGATCTGG AGCAGAAGCT GCAGGAGAAG TCCTCAGAGT TAGAGAAATG CAGTGAGCGC 
AGTGAAACGT CTAACGGTCG CCATCACGAG GACTCGAGCA GCAGTTTGGA GGAGGCTTTA 
CTACACCTGG AGGAGGAGCA ACAGAGGAGC AGCAGTTTAT CTGCAGTGAA CGCCATGCTG 
AGAGAACAGT TGGAGCAGGC CGGCTTGGCC AACGAGGCTC TGAGCCGGGA CATCCGGAGG 
CTCACCGCTG ATTGGACGAA AGCCAGAGAG GAGCTGGAAC AAAAGGAGTC GGACTGGAGG 
AGAGAGGAGG AGTCTTTCCA CAGCTACTTC AGCAGTGAAC ACGGTCGACT GCTGACCTTT 
TGGCGACAGG TGGTGGGGTT CAGGAGGCAC GTCTGTGAGA TGAAGAGCAG CGCTGAGAGG 
GACCTGTCAG ACATGCGGGG CGAGCTGGTT CGGATGTCCC ACTTGGTCCA GGTGTCCTGC 
GCGGGTCTGT CCGCCGTGCT GCGCAGTCAG GAAGCAGGAA CGGCTCTAGC CCTGGAGCAG 
GAGAGAGCTC TGAGGGATCA GCTGGAGCAG CAGCTGAGAG GTCAAGTCAA AGAGACGATG 
AACCTTCAGA GCAGAGCCGA TGCAGAGAGA AGTGAGCTGA GCCTCAGGCT TTCAGATGCG 
GCTCAAGAAA ATGAAAGACT AAAAAGTCAA ATGGAAGAAA GAGACCGTGA GATCGACAAT 
CTGAGCAGGA GGCTTGAGGA GCAGAGCCGC AGCGATGAGA CGGACTCCCA GCTGCTGAGG 
GCTCATTGCA ACGCTCTGTT GGACACGCTG AAGGACATCG CTCAGACGGT TCTGTCAGAC 
GGAGAGTCGT CTTCAGAGGC CGATCCGGAC AACTCGGCGG TCGTGCTGCA GGCGCTGATC 
CGTGACTCCT CCCCTCAACG CTCCTCCCAA ACCAGCTCCT CGTTGTCCCC TCTCCCGGAA 
GCGGCGCTGT CTGCGCTGCG TTCGGTGGTC ACAAACATGA TGCTGCAGCT GCAGGATGTT 
CGAGGTCGTC TGCTCTCTGC ACAGTCCTCT GCGCAGAAGT TTCGCAAGCA GCTGTCAGAA 
ATCGACTCTG CTAAAAGAGA CGCAGACCAA CGAAACCAAG CTCTGCAGCG AGACAAGGAC 
GCTGCTCTCA GAGAGCGGGA AACTGCCCAG AGGGAGAAGG AGCAACTGAA GCAAGAGAGA 
GACAAGCTGG CCAGTGAGAA GGCCAACCTG GAGAAGGCTG TTCAGGCGCT GCACAGCAGC 
AACCAGATGC TGCAGATGGA CTACGAGAAG CTCCAGCTGG CTGTGGCTTC TGCACAGCGA 
GAGCGAGACC GCGAGAGGGA GGAGAAGGAG GCTGCCATCC ATGACGGGGA GCGCTGGAAG 
ACGGAGATTC AGAGGATACA GAAGCAGAGG GACCAGAGTG AAAGTCGAGC CTCGGCTCAG 
CGGGGAGAAC TGTCTGCAGT GTTACAAACT CACCAGCAGG GGGAGGTGGA GAGGCAGCTG 
CTGGAACAGG AGAGTGCCCA CCTTTCAGAG GCCCTTGCTC GGGCTGCAAG CACCAACAAA 
GAGCTGTCGC TGCATCTGAG CAAGCTGCAG TATGAAGATG CAGCTCTCAG AGACTCTCTC 
ACAAAGATGG GAAACCTGAA TGAGGGTCTG GTCCAGGACA AGGTGGACCT TAACAAGTAT 
ATTCTGCAGC TTGAGGAGGA GAAGGCCGTC CTACAGGCTC AGAAACGGGA GGCGGAGCAG 
GAGAAACTGT CCATCAGGGA CGAGCTGGTC CGGCAGGAGC AGAACCGACT GGAGTTGGAG 
TCTTCCCGCG TCACACTGCA GCAGTCTCTG CAGGACACCG AGTTCAGTCG GGTGGGCGTG 
GAGGCAGAGC TGCAGAACCT CAGGGCTGAA CGACTGAAGC TACAGGAAAA GGTCGCCCAG 
TTGTGTGGTG AGGTGGCCTC TCTAGATTCA GAGTTGAGTC TCGCCAAGGG GGAGGGCCAG 
AGGAGCGAAA TGGCCTTGGA GGAAGCCGGC CGCAGTCGAG CAGAACTGAC CAAAGACAAA 
GCAGCTCTAG TGGTTCAGCT GACGGCATCT GAGAGAGAAA ACGCAATGCT AGCCGAGGAA 
CTGACAGCTT TCAGGTCGGA GCGGGAGACC CTGGAAACGA GTCTCTTTGA TGCGCAGCAG 
CAGCTTTTGC AGGCAGAGTC TTGCAGGGAA CAGCTGGAAG CAGAAAACCA AAACCTCCGC 
GTTCGCTGTG AAACTGCCAC AGTGGAGCTC AGGCGTTTGC GCTCAGATGC TGAGAATCAG 
CTGGCCCAGG CTGAGAGAGA CAAACAAGCG CTGACTCAAG CTCTCAGCGC CGCTCAGCTG 
GAGGCCCAGC AGGCTTCACG CAAGGCCACC TCCGAGCATC AGGAGGAGGT GGAGAGACTG 
GCCTTAGAGA AGGAAGTCGT GCGGCACAGC TTGCAGATGG AGCACGAGAC GGCTGTCCGG 
AAACTCCGAC AAGAAGTCGA AGATCAGCTC CAGAAAGCCA GGAGAGAAAA AGAGGAGCTG 
CAGGACGGAC TGAGGGCTCT GCAGCACGAG CGAGATCAGA GCCTGCTGCA GGCGGAAACG 
GAGAAACAAC AGGCCCTTTC ACTGAAGGAG GCAGAGAAAA CGGTTTTGTT GGAGAAGGTG 
TCCGGTCTGC AGGCTGATCT GTCAGCGCTG AGCCTGGAGG CTGAGCGGAC AGCCAGAGAA 
GCTGCCCAGT ACAAAGAGCA GGAGCAGATC AGAGTTGGGG CTCTAACCAG CGAACTGCAA 
GAACTTCGGT CACAGATGGA AGATGCAGCG GCCGGTCATG AAAGGGACAT CCTGAGTCTG 
AACGAAACCT GTGCAGACCT CCGTTCTCGT GCTGATGTTG CCCTCAAAGA GCTGGAGCAG 
TGCAGAGCTT CTCTCTCTGC TGCTGAGGAG AATCGAGAGC AGCTGAGGCG GGACTTCCTG 
GAGACGGAGC GCAGACTCAC CCAAACTCAG GAAGCGTCAG AAAACCACCG GAGGGAAGGA 
GCCGAGCTGC GGCGCAGCCT CTGTGATGTC ACCAGGGAGA GAGATGCTCT CAGCCAATCA 
AATGCTCAGC TGAGGGAAGC TCTCCGAAGC GCAGAAACGG AGAGAATAAG CGTGAAACGG 
CAGTGTGAGG AGAAGGAGCA GCGGCTGGTT GTGCTGGAGG AGAGCCTGTC CTCCTCGCAG 
AAGGAGGTGG CAGAGCTGCG GAGTTGCCTG AGGGAGGTGG AAAGGTCCCG CCTTGAGGCT 
CGCCGTGAGC TTCAGGAGCT TCGGAGACAG CTGAAGGTTC TGGATGCAGA CAGAGAGCAG 
AAAGGGAGGG AGCTGGCGGA GCTGCAAACC CGCTTGTCGT TGGAGGAGCA AAGAGAGGAG 
GAAAGAGGAA AGGAGGTTTT CATTTTAAAA CAGAAGCTCG CTGAAACCCA AACGGCCAGA 
GAGTCCCTCA AGAGAGAGCT CTCCATGACA CAGAAGCGTC TGGTGGAGTC CGAGTCCGGC 
TGGCGCAGCT GTGAGCGGGA GCTGACGGCA CAGCTGCAGG AGGCGCGAGG CTGCGAGAAG 
AAGCTCCAGG ATGAGGCCAA GAACCTGTCG GTGCGCGCGC AAGCGGCGCA GGAATCCGCC 
GCTCAGTGCA GCCTGCAGCT CAGCGAGGCG CAGGGGAGAC TGACCGCCAC CGAAGCTGAG 
CTGACGCGGG CGGAGGCCTC CAAGAGGGAT CTGGAGTTTC GCCTCAGCAG CCTCCAGTCG 
GCGCTGACGC GAACGCTGGG CATCGGGGCA AGCGGCAGGG CGAGCAGGGG GAGGAGTCCA 
GGCGGGAGCT CTGCATCACC TGGCAGTATC TCCCGTCACC ACAGCATCTC ACCTTTGCGC 
CCTTCGCTGT CGCCCCCTAA AGAATTCAGA GGAAGCACGC CTGATAACAC CATGGGATCA 
GGAGCTGTGT CTCCTGAAGG AGGCGGTACT CCTTTGCCTC CCGCTCAGCC AGAGCTGGAT 
CCCGACACGC TGCGCAGTGG CCTCAGAGAC TTCCTGCAGG AGCTGCGAGA CGCACACAGA 
CAAAGGGATG ATGCCCAATG CCAGCTGATG ACTCTGCAGC GGGAGCTGGA GGAGCAGAAG 
CTGGAGCGAG AATGTGCCGA GAACCGCTCC ACTCAGCTGC AAAACAGCCT GAAGGAATGC 
CAAGAAGGGA AGCGTGGACT TGACGACCGT CTGACCAGCA CTCAGATTCT CCTGCAGCAG 
CAGGAGGATG CGGTGAAGAG AGTGGACAGA GAGAGGAGAG CTCTTGCCGA CAGGGTGAGG 
GAGTTGGAGC GCTCGCTGCA CACCGCAGAG ATGGAGAAGA AGCACACTCA GGAGCAGTTG 
AATAAGCAGC GGGCCACTGA GGCACGCCTG GAGGCGGAGA GGAAGCGCCT GCGGGAGGCT 
CTGGAGGCCG CCGAAGCTCG AGCCACCCGG GTGGAGCTGG GGAGGCGCAG CCTGGAAGGG 
GAGCTGCAGA GGCTCAAACT CGGTCTGGGA GACCGGGAGG CTGAAAGCCA GGCCTCACAG 
GAGCGCCACG ACTGTTTGCT CAGACAGGTG GCGGAAGGAG AAGCACAAGT CTCTCTGCTG 
CAGACAGAGG TGGACAGACT GAGTCAAGCT CTGCTGAAAG CTCAGGAAGG GGAAGCTGCT 
CTGAAGCAGA AGGTCACGAC TCTCAGCCAG AGCCTCCAGG AGGCTGCAGC TGATCACAGC 
AGCACGCAGA CTCGCCTCAC TGCTCTGCAG AAAACACTGA GCTTGGTGGA ACAGGACAAA 
AGACTTCTGC AGGAGAAGAT GGATGAAGCC CGCACTTCTG TAACTGAAGC AAAGAGAAGC 
ATGTTTGCTC TCACTGAGCG CGTGCAGAGC CTCCAGGGTG AGCTGACCCA GAGTGAACTG 
CTGAGAGAGG AGCTGGAGGC GGAGCTTAAG AACACACAGG AGGCACTCCG ACAGCGCTCA 
GGAAGTTTGG CAGAGACTCA GCACAGCATC CAGAAAGCTC AGACGGAGCG GGCAGCTGTG 
GAGGAGCGTC TGCTCGCACT GCAAAGAGCT GTGGCTCTGC TGGAGACAGA GAAAAAAGAT 
GCAGAGAGGC AAGCTGCAAG GCTGGAGAAG GACAAGGATG CACTGAGGAA CACGTTGGAT 
AAGGTTGAAA GGCAGAAACT GAAGTGTGAG GAGGGCAGCA TGCGGCTCTC AGCAGAGAAG 
ACCCGGCTGG ACCGCTCCCT GAACGCAGTG GAGCAGGAGC TGCAGGAGGC GCAGCAGCAG 
ATGCTGATGC TGCAGACTCA GCTGGCTGAG ATGGAGCAGG CCCACAGTGG GTGTGAGGGT 
TTGCTGCGAC AGCGGGATGA AGCTCAGCAG GAGGCGGAGA AACTGAGGAA CAGCTGTAAG 
GACCTGGAAC GCACTCTGAG CACCAGAGAA CGAGCCCACC GGCACCGAGT CAAAGGACTG 
GAGGAGCAGG TGTCCACTTT GAAGGAGCAG CTGCAGCAGG AGATGAAACG GCGGCAGCCT 
CCTCTTCCGT CCACGTTTAC GTCCACTGGG ATCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

crocc ( XM_020701419.1 ) cDNA ORF clone, Oryzias latipes(Japanese medaka) -> XP_020557078.1 Oryzias latipes ciliary rootlet coiled-coil, rootletin (crocc), transcript variant X2, mRNA.

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CloneID	OJa25265
Clone ID Related Accession (Same CDS sequence)	XM_020701419.2 , XM_020701419.1
Accession Version	XM_020701419.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6036bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1491408000000
Organism	Oryzias latipes(Japanese medaka)
Product	rootletin isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_004095157.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Oryzias latipes Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCGCTG CGGAAAACTC CGACAGCGAC CCCAGCAAGC TGGAGTCAGT GATCCGGAGG 
CTGGAGGAAA GTGTTCTGTC TGAGGAGAAG AGGTTGACGG TTCGAGGCCC TTCACCGGAT 
GACCCCCCTG CGTGTCTGCC TGCCAGAGTG CGAGAGATCG TCACGAAGAA CCTCAGTGAC 
AGCAGGGAGA TGTCCTCGCT CATGTCCCTC CAGGAGGAGA ACCGGGTGCT GCAGGGCGAG 
CTGGCCCGGC TGGAGGACCT GCTGGCGCAC AGCAGGGCGG ATCGGGACGA GCTCGCCATC 
AAGTACGGGG CAATCAGTGA ACGGCTGGAG CAGGCTCTAC GCTCAGAAAC GGGGGAGGGA 
GAGGCCGACT CACAGGCGTC CCGCAGCCTG GCTCAGCAGA ATGTGGAATT GCGCCGACGG 
CTGGACGAGG AACAGGCTGC ATACAGGCGC AAACTCACCG CCTACCAAGA AGGCCAGCAG 
AGGCAGGCGC AGCTGGTGCA GAAGCTTCAG GCCAAGGTGC TTCAGTACAA AAAGAGATGT 
GGGGATCTGG AGCAGAAGCT GCAGGAGAAG TCCTCAGAGT TAGAGAAATG CAGTGAGCGC 
AGTGAAACGT CTAACGGTCG CCATCACGAG GACTCGAGCA GCAGTTTGGA GGAGGCTTTA 
CTACACCTGG AGGAGGAGCA ACAGAGGAGC AGCAGTTTAT CTGCAGTGAA CGCCATGCTG 
AGAGAACAGT TGGAGCAGGC CGGCTTGGCC AACGAGGCTC TGAGCCGGGA CATCCGGAGG 
CTCACCGCTG ATTGGACGAA AGCCAGAGAG GAGCTGGAAC AAAAGGAGTC GGACTGGAGG 
AGAGAGGAGG AGTCTTTCCA CAGCTACTTC AGCAGTGAAC ACGGTCGACT GCTGACCTTT 
TGGCGACAGG TGGTGGGGTT CAGGAGGCAC GTCTGTGAGA TGAAGAGCAG CGCTGAGAGG 
GACCTGTCAG ACATGCGGGG CGAGCTGGTT CGGATGTCCC ACTTGGTCCA GGTGTCCTGC 
GCGGGTCTGT CCGCCGTGCT GCGCAGTCAG GAAGCAGGAA CGGCTCTAGC CCTGGAGCAG 
GAGAGAGCTC TGAGGGATCA GCTGGAGCAG CAGCTGAGAG GTCAAGTCAA AGAGACGATG 
AACCTTCAGA GCAGAGCCGA TGCAGAGAGA AGTGAGCTGA GCCTCAGGCT TTCAGATGCG 
GCTCAAGAAA ATGAAAGACT AAAAAGTCAA ATGGAAGAAA GAGACCGTGA GATCGACAAT 
CTGAGCAGGA GGCTTGAGGA GCAGAGCCGC AGCGATGAGA CGGACTCCCA GCTGCTGAGG 
GCTCATTGCA ACGCTCTGTT GGACACGCTG AAGGACATCG CTCAGACGGT TCTGTCAGAC 
GGAGAGTCGT CTTCAGAGGC CGATCCGGAC AACTCGGCGG TCGTGCTGCA GGCGCTGATC 
CGTGACTCCT CCCCTCAACG CTCCTCCCAA ACCAGCTCCT CGTTGTCCCC TCTCCCGGAA 
GCGGCGCTGT CTGCGCTGCG TTCGGTGGTC ACAAACATGA TGCTGCAGCT GCAGGATGTT 
CGAGGTCGTC TGCTCTCTGC ACAGTCCTCT GCGCAGAAGT TTCGCAAGCA GCTGTCAGAA 
ATCGACTCTG CTAAAAGAGA CGCAGACCAA CGAAACCAAG CTCTGCAGCG AGACAAGGAC 
GCTGCTCTCA GAGAGCGGGA AACTGCCCAG AGGGAGAAGG AGCAACTGAA GCAAGAGAGA 
GACAAGCTGG CCAGTGAGAA GGCCAACCTG GAGAAGGCTG TTCAGGCGCT GCACAGCAGC 
AACCAGATGC TGCAGATGGA CTACGAGAAG CTCCAGCTGG CTGTGGCTTC TGCACAGCGA 
GAGCGAGACC GCGAGAGGGA GGAGAAGGAG GCTGCCATCC ATGACGGGGA GCGCTGGAAG 
ACGGAGATTC AGAGGATACA GAAGCAGAGG GACCAGAGTG AAAGTCGAGC CTCGGCTCAG 
CGGGGAGAAC TGTCTGCAGT GTTACAAACT CACCAGCAGG GGGAGGTGGA GAGGCAGCTG 
CTGGAACAGG AGAGTGCCCA CCTTTCAGAG GCCCTTGCTC GGGCTGCAAG CACCAACAAA 
GAGCTGTCGC TGCATCTGAG CAAGCTGCAG TATGAAGATG CAGCTCTCAG AGACTCTCTC 
ACAAAGATGG GAAACCTGAA TGAGGGTCTG GTCCAGGACA AGGTGGACCT TAACAAGTAT 
ATTCTGCAGC TTGAGGAGGA GAAGGCCGTC CTACAGGCTC AGAAACGGGA GGCGGAGCAG 
GAGAAACTGT CCATCAGGGA CGAGCTGGTC CGGCAGGAGC AGAACCGACT GGAGTTGGAG 
TCTTCCCGCG TCACACTGCA GCAGTCTCTG CAGGACACCG AGTTCAGTCG GGTGGGCGTG 
GAGGCAGAGC TGCAGAACCT CAGGGCTGAA CGACTGAAGC TACAGGAAAA GGTCGCCCAG 
TTGTGTGGTG AGGTGGCCTC TCTAGATTCA GAGTTGAGTC TCGCCAAGGG GGAGGGCCAG 
AGGAGCGAAA TGGCCTTGGA GGAAGCCGGC CGCAGTCGAG CAGAACTGAC CAAAGACAAA 
GCAGCTCTAG TGGTTCAGCT GACGGCATCT GAGAGAGAAA ACGCAATGCT AGCCGAGGAA 
CTGACAGCTT TCAGGTCGGA GCGGGAGACC CTGGAAACGA GTCTCTTTGA TGCGCAGCAG 
CAGCTTTTGC AGGCAGAGTC TTGCAGGGAA CAGCTGGAAG CAGAAAACCA AAACCTCCGC 
GTTCGCTGTG AAACTGCCAC AGTGGAGCTC AGGCGTTTGC GCTCAGATGC TGAGAATCAG 
CTGGCCCAGG CTGAGAGAGA CAAACAAGCG CTGACTCAAG CTCTCAGCGC CGCTCAGCTG 
GAGGCCCAGC AGGCTTCACG CAAGGCCACC TCCGAGCATC AGGAGGAGGT GGAGAGACTG 
GCCTTAGAGA AGGAAGTCGT GCGGCACAGC TTGCAGATGG AGCACGAGAC GGCTGTCCGG 
AAACTCCGAC AAGAAGTCGA AGATCAGCTC CAGAAAGCCA GGAGAGAAAA AGAGGAGCTG 
CAGGACGGAC TGAGGGCTCT GCAGCACGAG CGAGATCAGA GCCTGCTGCA GGCGGAAACG 
GAGAAACAAC AGGCCCTTTC ACTGAAGGAG GCAGAGAAAA CGGTTTTGTT GGAGAAGGTG 
TCCGGTCTGC AGGCTGATCT GTCAGCGCTG AGCCTGGAGG CTGAGCGGAC AGCCAGAGAA 
GCTGCCCAGT ACAAAGAGCA GGAGCAGATC AGAGTTGGGG CTCTAACCAG CGAACTGCAA 
GAACTTCGGT CACAGATGGA AGATGCAGCG GCCGGTCATG AAAGGGACAT CCTGAGTCTG 
AACGAAACCT GTGCAGACCT CCGTTCTCGT GCTGATGTTG CCCTCAAAGA GCTGGAGCAG 
TGCAGAGCTT CTCTCTCTGC TGCTGAGGAG AATCGAGAGC AGCTGAGGCG GGACTTCCTG 
GAGACGGAGC GCAGACTCAC CCAAACTCAG GAAGCGTCAG AAAACCACCG GAGGGAAGGA 
GCCGAGCTGC GGCGCAGCCT CTGTGATGTC ACCAGGGAGA GAGATGCTCT CAGCCAATCA 
AATGCTCAGC TGAGGGAAGC TCTCCGAAGC GCAGAAACGG AGAGAATAAG CGTGAAACGG 
CAGTGTGAGG AGAAGGAGCA GCGGCTGGTT GTGCTGGAGG AGAGCCTGTC CTCCTCGCAG 
AAGGAGGTGG CAGAGCTGCG GAGTTGCCTG AGGGAGGTGG AAAGGTCCCG CCTTGAGGCT 
CGCCGTGAGC TTCAGGAGCT TCGGAGACAG CTGAAGGTTC TGGATGCAGA CAGAGAGCAG 
AAAGGGAGGG AGCTGGCGGA GCTGCAAACC CGCTTGTCGT TGGAGGAGCA AAGAGAGGAG 
GAAAGAGGAA AGGAGGTTTT CATTTTAAAA CAGAAGCTCG CTGAAACCCA AACGGCCAGA 
GAGTCCCTCA AGAGAGAGCT CTCCATGACA CAGAAGCGTC TGGTGGAGTC CGAGTCCGGC 
TGGCGCAGCT GTGAGCGGGA GCTGACGGCA CAGCTGCAGG AGGCGCGAGG CTGCGAGAAG 
AAGCTCCAGG ATGAGGCCAA GAACCTGTCG GTGCGCGCGC AAGCGGCGCA GGAATCCGCC 
GCTCAGTGCA GCCTGCAGCT CAGCGAGGCG CAGGGGAGAC TGACCGCCAC CGAAGCTGAG 
CTGACGCGGG CGGAGGCCTC CAAGAGGGAT CTGGAGTTTC GCCTCAGCAG CCTCCAGTCG 
GCGCTGACGC GAACGCTGGG CATCGGGGCA AGCGGCAGGG CGAGCAGGGG GAGGAGTCCA 
GGCGGGAGCT CTGCATCACC TGGCAGTATC TCCCGTCACC ACAGCATCTC ACCTTTGCGC 
CCTTCGCTGT CGCCCCCTAA AGAATTCAGA GGAAGCACGC CTGATAACAC CATGGGATCA 
GGAGCTGTGT CTCCTGAAGG AGGCGGTACT CCTTTGCCTC CCGCTCAGCC AGAGCTGGAT 
CCCGACACGC TGCGCAGTGG CCTCAGAGAC TTCCTGCAGG AGCTGCGAGA CGCACACAGA 
CAAAGGGATG ATGCCCAATG CCAGCTGATG ACTCTGCAGC GGGAGCTGGA GGAGCAGAAG 
CTGGAGCGAG AATGTGCCGA GAACCGCTCC ACTCAGCTGC AAAACAGCCT GAAGGAATGC 
CAAGAAGGGA AGCGTGGACT TGACGACCGT CTGACCAGCA CTCAGATTCT CCTGCAGCAG 
CAGGAGGATG CGGTGAAGAG AGTGGACAGA GAGAGGAGAG CTCTTGCCGA CAGGGTGAGG 
GAGTTGGAGC GCTCGCTGCA CACCGCAGAG ATGGAGAAGA AGCACACTCA GGAGCAGTTG 
AATAAGCAGC GGGCCACTGA GGCACGCCTG GAGGCGGAGA GGAAGCGCCT GCGGGAGGCT 
CTGGAGGCCG CCGAAGCTCG AGCCACCCGG GTGGAGCTGG GGAGGCGCAG CCTGGAAGGG 
GAGCTGCAGA GGCTCAAACT CGGTCTGGGA GACCGGGAGG CTGAAAGCCA GGCCTCACAG 
GAGCGCCACG ACTGTTTGCT CAGACAGGTG GCGGAAGGAG AAGCACAAGT CTCTCTGCTG 
CAGACAGAGG TGGACAGACT GAGTCAAGCT CTGCTGAAAG CTCAGGAAGG GGAAGCTGCT 
CTGAAGCAGA AGGTCACGAC TCTCAGCCAG AGCCTCCAGG AGGCTGCAGC TGATCACAGC 
AGCACGCAGA CTCGCCTCAC TGCTCTGCAG AAAACACTGA GCTTGGTGGA ACAGGACAAA 
AGACTTCTGC AGGAGAAGAT GGATGAAGCC CGCACTTCTG TAACTGAAGC AAAGAGAAGC 
ATGTTTGCTC TCACTGAGCG CGTGCAGAGC CTCCAGGGTG AGCTGACCCA GAGTGAACTG 
CTGAGAGAGG AGCTGGAGGC GGAGCTTAAG AACACACAGG AGGCACTCCG ACAGCGCTCA 
GGAAGTTTGG CAGAGACTCA GCACAGCATC CAGAAAGCTC AGACGGAGCG GGCAGCTGTG 
GAGGAGCGTC TGCTCGCACT GCAAAGAGCT GTGGCTCTGC TGGAGACAGA GAAAAAAGAT 
GCAGAGAGGC AAGCTGCAAG GCTGGAGAAG GACAAGGATG CACTGAGGAA CACGTTGGAT 
AAGGTTGAAA GGCAGAAACT GAAGTGTGAG GAGGGCAGCA TGCGGCTCTC AGCAGAGAAG 
ACCCGGCTGG ACCGCTCCCT GAACGCAGTG GAGCAGGAGC TGCAGGAGGC GCAGCAGCAG 
ATGCTGATGC TGCAGACTCA GCTGGCTGAG ATGGAGCAGG CCCACAGTGG GTGTGAGGGT 
TTGCTGCGAC AGCGGGATGA AGCTCAGCAG GAGGCGGAGA AACTGAGGAA CAGCTGTAAG 
GACCTGGAAC GCACTCTGAG CACCAGAGAA CGAGCCCACC GGCACCGAGT CAAAGGACTG 
GAGGAGCAGG TGTCCACTTT GAAGGAGCAG CTGCAGCAGG AGATGAAACG GCGGCAGCCT 
CCTCTTCCGT CCACGTTTAC GTCCACTGGG ATCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_020557078.1
CDS	189..6224
Translation	MSAAENSDSDPSKLESVIRRLEESVLSEEKRLTVRGPSPDDPPACLPARVREIVTKNLSDSREMSSLMSLQEENRVLQGELARLEDLLAHSRADRDELAIKYGAISERLEQALRSETGEGEADSQASRSLAQQNVELRRRLDEEQAAYRRKLTAYQEGQQRQAQLVQKLQAKVLQYKKRCGDLEQKLQEKSSELEKCSERSETSNGRHHEDSSSSLEEALLHLEEEQQRSSSLSAVNAMLREQLEQAGLANEALSRDIRRLTADWTKAREELEQKESDWRREEESFHSYFSSEHGRLLTFWRQVVGFRRHVCEMKSSAERDLSDMRGELVRMSHLVQVSCAGLSAVLRSQEAGTALALEQERALRDQLEQQLRGQVKETMNLQSRADAERSELSLRLSDAAQENERLKSQMEERDREIDNLSRRLEEQSRSDETDSQLLRAHCNALLDTLKDIAQTVLSDGESSSEADPDNSAVVLQALIRDSSPQRSSQTSSSLSPLPEAALSALRSVVTNMMLQLQDVRGRLLSAQSSAQKFRKQLSEIDSAKRDADQRNQALQRDKDAALRERETAQREKEQLKQERDKLASEKANLEKAVQALHSSNQMLQMDYEKLQLAVASAQRERDREREEKEAAIHDGERWKTEIQRIQKQRDQSESRASAQRGELSAVLQTHQQGEVERQLLEQESAHLSEALARAASTNKELSLHLSKLQYEDAALRDSLTKMGNLNEGLVQDKVDLNKYILQLEEEKAVLQAQKREAEQEKLSIRDELVRQEQNRLELESSRVTLQQSLQDTEFSRVGVEAELQNLRAERLKLQEKVAQLCGEVASLDSELSLAKGEGQRSEMALEEAGRSRAELTKDKAALVVQLTASERENAMLAEELTAFRSERETLETSLFDAQQQLLQAESCREQLEAENQNLRVRCETATVELRRLRSDAENQLAQAERDKQALTQALSAAQLEAQQASRKATSEHQEEVERLALEKEVVRHSLQMEHETAVRKLRQEVEDQLQKARREKEELQDGLRALQHERDQSLLQAETEKQQALSLKEAEKTVLLEKVSGLQADLSALSLEAERTAREAAQYKEQEQIRVGALTSELQELRSQMEDAAAGHERDILSLNETCADLRSRADVALKELEQCRASLSAAEENREQLRRDFLETERRLTQTQEASENHRREGAELRRSLCDVTRERDALSQSNAQLREALRSAETERISVKRQCEEKEQRLVVLEESLSSSQKEVAELRSCLREVERSRLEARRELQELRRQLKVLDADREQKGRELAELQTRLSLEEQREEERGKEVFILKQKLAETQTARESLKRELSMTQKRLVESESGWRSCERELTAQLQEARGCEKKLQDEAKNLSVRAQAAQESAAQCSLQLSEAQGRLTATEAELTRAEASKRDLEFRLSSLQSALTRTLGIGASGRASRGRSPGGSSASPGSISRHHSISPLRPSLSPPKEFRGSTPDNTMGSGAVSPEGGGTPLPPAQPELDPDTLRSGLRDFLQELRDAHRQRDDAQCQLMTLQRELEEQKLERECAENRSTQLQNSLKECQEGKRGLDDRLTSTQILLQQQEDAVKRVDRERRALADRVRELERSLHTAEMEKKHTQEQLNKQRATEARLEAERKRLREALEAAEARATRVELGRRSLEGELQRLKLGLGDREAESQASQERHDCLLRQVAEGEAQVSLLQTEVDRLSQALLKAQEGEAALKQKVTTLSQSLQEAAADHSSTQTRLTALQKTLSLVEQDKRLLQEKMDEARTSVTEAKRSMFALTERVQSLQGELTQSELLREELEAELKNTQEALRQRSGSLAETQHSIQKAQTERAAVEERLLALQRAVALLETEKKDAERQAARLEKDKDALRNTLDKVERQKLKCEEGSMRLSAEKTRLDRSLNAVEQELQEAQQQMLMLQTQLAEMEQAHSGCEGLLRQRDEAQQEAEKLRNSCKDLERTLSTRERAHRHRVKGLEEQVSTLKEQLQQEMKRRQPPLPSTFTSTGI

Target ORF information:

RefSeq Version	XM_020701419.1
Organism	Oryzias latipes(Japanese medaka)
Definition	Oryzias latipes ciliary rootlet coiled-coil, rootletin (crocc), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_020701419.1

ORF Insert Sequence:

ATGAGCGCTG CGGAAAACTC CGACAGCGAC CCCAGCAAGC TGGAGTCAGT GATCCGGAGG 
CTGGAGGAAA GTGTTCTGTC TGAGGAGAAG AGGTTGACGG TTCGAGGCCC TTCACCGGAT 
GACCCCCCTG CGTGTCTGCC TGCCAGAGTG CGAGAGATCG TCACGAAGAA CCTCAGTGAC 
AGCAGGGAGA TGTCCTCGCT CATGTCCCTC CAGGAGGAGA ACCGGGTGCT GCAGGGCGAG 
CTGGCCCGGC TGGAGGACCT GCTGGCGCAC AGCAGGGCGG ATCGGGACGA GCTCGCCATC 
AAGTACGGGG CAATCAGTGA ACGGCTGGAG CAGGCTCTAC GCTCAGAAAC GGGGGAGGGA 
GAGGCCGACT CACAGGCGTC CCGCAGCCTG GCTCAGCAGA ATGTGGAATT GCGCCGACGG 
CTGGACGAGG AACAGGCTGC ATACAGGCGC AAACTCACCG CCTACCAAGA AGGCCAGCAG 
AGGCAGGCGC AGCTGGTGCA GAAGCTTCAG GCCAAGGTGC TTCAGTACAA AAAGAGATGT 
GGGGATCTGG AGCAGAAGCT GCAGGAGAAG TCCTCAGAGT TAGAGAAATG CAGTGAGCGC 
AGTGAAACGT CTAACGGTCG CCATCACGAG GACTCGAGCA GCAGTTTGGA GGAGGCTTTA 
CTACACCTGG AGGAGGAGCA ACAGAGGAGC AGCAGTTTAT CTGCAGTGAA CGCCATGCTG 
AGAGAACAGT TGGAGCAGGC CGGCTTGGCC AACGAGGCTC TGAGCCGGGA CATCCGGAGG 
CTCACCGCTG ATTGGACGAA AGCCAGAGAG GAGCTGGAAC AAAAGGAGTC GGACTGGAGG 
AGAGAGGAGG AGTCTTTCCA CAGCTACTTC AGCAGTGAAC ACGGTCGACT GCTGACCTTT 
TGGCGACAGG TGGTGGGGTT CAGGAGGCAC GTCTGTGAGA TGAAGAGCAG CGCTGAGAGG 
GACCTGTCAG ACATGCGGGG CGAGCTGGTT CGGATGTCCC ACTTGGTCCA GGTGTCCTGC 
GCGGGTCTGT CCGCCGTGCT GCGCAGTCAG GAAGCAGGAA CGGCTCTAGC CCTGGAGCAG 
GAGAGAGCTC TGAGGGATCA GCTGGAGCAG CAGCTGAGAG GTCAAGTCAA AGAGACGATG 
AACCTTCAGA GCAGAGCCGA TGCAGAGAGA AGTGAGCTGA GCCTCAGGCT TTCAGATGCG 
GCTCAAGAAA ATGAAAGACT AAAAAGTCAA ATGGAAGAAA GAGACCGTGA GATCGACAAT 
CTGAGCAGGA GGCTTGAGGA GCAGAGCCGC AGCGATGAGA CGGACTCCCA GCTGCTGAGG 
GCTCATTGCA ACGCTCTGTT GGACACGCTG AAGGACATCG CTCAGACGGT TCTGTCAGAC 
GGAGAGTCGT CTTCAGAGGC CGATCCGGAC AACTCGGCGG TCGTGCTGCA GGCGCTGATC 
CGTGACTCCT CCCCTCAACG CTCCTCCCAA ACCAGCTCCT CGTTGTCCCC TCTCCCGGAA 
GCGGCGCTGT CTGCGCTGCG TTCGGTGGTC ACAAACATGA TGCTGCAGCT GCAGGATGTT 
CGAGGTCGTC TGCTCTCTGC ACAGTCCTCT GCGCAGAAGT TTCGCAAGCA GCTGTCAGAA 
ATCGACTCTG CTAAAAGAGA CGCAGACCAA CGAAACCAAG CTCTGCAGCG AGACAAGGAC 
GCTGCTCTCA GAGAGCGGGA AACTGCCCAG AGGGAGAAGG AGCAACTGAA GCAAGAGAGA 
GACAAGCTGG CCAGTGAGAA GGCCAACCTG GAGAAGGCTG TTCAGGCGCT GCACAGCAGC 
AACCAGATGC TGCAGATGGA CTACGAGAAG CTCCAGCTGG CTGTGGCTTC TGCACAGCGA 
GAGCGAGACC GCGAGAGGGA GGAGAAGGAG GCTGCCATCC ATGACGGGGA GCGCTGGAAG 
ACGGAGATTC AGAGGATACA GAAGCAGAGG GACCAGAGTG AAAGTCGAGC CTCGGCTCAG 
CGGGGAGAAC TGTCTGCAGT GTTACAAACT CACCAGCAGG GGGAGGTGGA GAGGCAGCTG 
CTGGAACAGG AGAGTGCCCA CCTTTCAGAG GCCCTTGCTC GGGCTGCAAG CACCAACAAA 
GAGCTGTCGC TGCATCTGAG CAAGCTGCAG TATGAAGATG CAGCTCTCAG AGACTCTCTC 
ACAAAGATGG GAAACCTGAA TGAGGGTCTG GTCCAGGACA AGGTGGACCT TAACAAGTAT 
ATTCTGCAGC TTGAGGAGGA GAAGGCCGTC CTACAGGCTC AGAAACGGGA GGCGGAGCAG 
GAGAAACTGT CCATCAGGGA CGAGCTGGTC CGGCAGGAGC AGAACCGACT GGAGTTGGAG 
TCTTCCCGCG TCACACTGCA GCAGTCTCTG CAGGACACCG AGTTCAGTCG GGTGGGCGTG 
GAGGCAGAGC TGCAGAACCT CAGGGCTGAA CGACTGAAGC TACAGGAAAA GGTCGCCCAG 
TTGTGTGGTG AGGTGGCCTC TCTAGATTCA GAGTTGAGTC TCGCCAAGGG GGAGGGCCAG 
AGGAGCGAAA TGGCCTTGGA GGAAGCCGGC CGCAGTCGAG CAGAACTGAC CAAAGACAAA 
GCAGCTCTAG TGGTTCAGCT GACGGCATCT GAGAGAGAAA ACGCAATGCT AGCCGAGGAA 
CTGACAGCTT TCAGGTCGGA GCGGGAGACC CTGGAAACGA GTCTCTTTGA TGCGCAGCAG 
CAGCTTTTGC AGGCAGAGTC TTGCAGGGAA CAGCTGGAAG CAGAAAACCA AAACCTCCGC 
GTTCGCTGTG AAACTGCCAC AGTGGAGCTC AGGCGTTTGC GCTCAGATGC TGAGAATCAG 
CTGGCCCAGG CTGAGAGAGA CAAACAAGCG CTGACTCAAG CTCTCAGCGC CGCTCAGCTG 
GAGGCCCAGC AGGCTTCACG CAAGGCCACC TCCGAGCATC AGGAGGAGGT GGAGAGACTG 
GCCTTAGAGA AGGAAGTCGT GCGGCACAGC TTGCAGATGG AGCACGAGAC GGCTGTCCGG 
AAACTCCGAC AAGAAGTCGA AGATCAGCTC CAGAAAGCCA GGAGAGAAAA AGAGGAGCTG 
CAGGACGGAC TGAGGGCTCT GCAGCACGAG CGAGATCAGA GCCTGCTGCA GGCGGAAACG 
GAGAAACAAC AGGCCCTTTC ACTGAAGGAG GCAGAGAAAA CGGTTTTGTT GGAGAAGGTG 
TCCGGTCTGC AGGCTGATCT GTCAGCGCTG AGCCTGGAGG CTGAGCGGAC AGCCAGAGAA 
GCTGCCCAGT ACAAAGAGCA GGAGCAGATC AGAGTTGGGG CTCTAACCAG CGAACTGCAA 
GAACTTCGGT CACAGATGGA AGATGCAGCG GCCGGTCATG AAAGGGACAT CCTGAGTCTG 
AACGAAACCT GTGCAGACCT CCGTTCTCGT GCTGATGTTG CCCTCAAAGA GCTGGAGCAG 
TGCAGAGCTT CTCTCTCTGC TGCTGAGGAG AATCGAGAGC AGCTGAGGCG GGACTTCCTG 
GAGACGGAGC GCAGACTCAC CCAAACTCAG GAAGCGTCAG AAAACCACCG GAGGGAAGGA 
GCCGAGCTGC GGCGCAGCCT CTGTGATGTC ACCAGGGAGA GAGATGCTCT CAGCCAATCA 
AATGCTCAGC TGAGGGAAGC TCTCCGAAGC GCAGAAACGG AGAGAATAAG CGTGAAACGG 
CAGTGTGAGG AGAAGGAGCA GCGGCTGGTT GTGCTGGAGG AGAGCCTGTC CTCCTCGCAG 
AAGGAGGTGG CAGAGCTGCG GAGTTGCCTG AGGGAGGTGG AAAGGTCCCG CCTTGAGGCT 
CGCCGTGAGC TTCAGGAGCT TCGGAGACAG CTGAAGGTTC TGGATGCAGA CAGAGAGCAG 
AAAGGGAGGG AGCTGGCGGA GCTGCAAACC CGCTTGTCGT TGGAGGAGCA AAGAGAGGAG 
GAAAGAGGAA AGGAGGTTTT CATTTTAAAA CAGAAGCTCG CTGAAACCCA AACGGCCAGA 
GAGTCCCTCA AGAGAGAGCT CTCCATGACA CAGAAGCGTC TGGTGGAGTC CGAGTCCGGC 
TGGCGCAGCT GTGAGCGGGA GCTGACGGCA CAGCTGCAGG AGGCGCGAGG CTGCGAGAAG 
AAGCTCCAGG ATGAGGCCAA GAACCTGTCG GTGCGCGCGC AAGCGGCGCA GGAATCCGCC 
GCTCAGTGCA GCCTGCAGCT CAGCGAGGCG CAGGGGAGAC TGACCGCCAC CGAAGCTGAG 
CTGACGCGGG CGGAGGCCTC CAAGAGGGAT CTGGAGTTTC GCCTCAGCAG CCTCCAGTCG 
GCGCTGACGC GAACGCTGGG CATCGGGGCA AGCGGCAGGG CGAGCAGGGG GAGGAGTCCA 
GGCGGGAGCT CTGCATCACC TGGCAGTATC TCCCGTCACC ACAGCATCTC ACCTTTGCGC 
CCTTCGCTGT CGCCCCCTAA AGAATTCAGA GGAAGCACGC CTGATAACAC CATGGGATCA 
GGAGCTGTGT CTCCTGAAGG AGGCGGTACT CCTTTGCCTC CCGCTCAGCC AGAGCTGGAT 
CCCGACACGC TGCGCAGTGG CCTCAGAGAC TTCCTGCAGG AGCTGCGAGA CGCACACAGA 
CAAAGGGATG ATGCCCAATG CCAGCTGATG ACTCTGCAGC GGGAGCTGGA GGAGCAGAAG 
CTGGAGCGAG AATGTGCCGA GAACCGCTCC ACTCAGCTGC AAAACAGCCT GAAGGAATGC 
CAAGAAGGGA AGCGTGGACT TGACGACCGT CTGACCAGCA CTCAGATTCT CCTGCAGCAG 
CAGGAGGATG CGGTGAAGAG AGTGGACAGA GAGAGGAGAG CTCTTGCCGA CAGGGTGAGG 
GAGTTGGAGC GCTCGCTGCA CACCGCAGAG ATGGAGAAGA AGCACACTCA GGAGCAGTTG 
AATAAGCAGC GGGCCACTGA GGCACGCCTG GAGGCGGAGA GGAAGCGCCT GCGGGAGGCT 
CTGGAGGCCG CCGAAGCTCG AGCCACCCGG GTGGAGCTGG GGAGGCGCAG CCTGGAAGGG 
GAGCTGCAGA GGCTCAAACT CGGTCTGGGA GACCGGGAGG CTGAAAGCCA GGCCTCACAG 
GAGCGCCACG ACTGTTTGCT CAGACAGGTG GCGGAAGGAG AAGCACAAGT CTCTCTGCTG 
CAGACAGAGG TGGACAGACT GAGTCAAGCT CTGCTGAAAG CTCAGGAAGG GGAAGCTGCT 
CTGAAGCAGA AGGTCACGAC TCTCAGCCAG AGCCTCCAGG AGGCTGCAGC TGATCACAGC 
AGCACGCAGA CTCGCCTCAC TGCTCTGCAG AAAACACTGA GCTTGGTGGA ACAGGACAAA 
AGACTTCTGC AGGAGAAGAT GGATGAAGCC CGCACTTCTG TAACTGAAGC AAAGAGAAGC 
ATGTTTGCTC TCACTGAGCG CGTGCAGAGC CTCCAGGGTG AGCTGACCCA GAGTGAACTG 
CTGAGAGAGG AGCTGGAGGC GGAGCTTAAG AACACACAGG AGGCACTCCG ACAGCGCTCA 
GGAAGTTTGG CAGAGACTCA GCACAGCATC CAGAAAGCTC AGACGGAGCG GGCAGCTGTG 
GAGGAGCGTC TGCTCGCACT GCAAAGAGCT GTGGCTCTGC TGGAGACAGA GAAAAAAGAT 
GCAGAGAGGC AAGCTGCAAG GCTGGAGAAG GACAAGGATG CACTGAGGAA CACGTTGGAT 
AAGGTTGAAA GGCAGAAACT GAAGTGTGAG GAGGGCAGCA TGCGGCTCTC AGCAGAGAAG 
ACCCGGCTGG ACCGCTCCCT GAACGCAGTG GAGCAGGAGC TGCAGGAGGC GCAGCAGCAG 
ATGCTGATGC TGCAGACTCA GCTGGCTGAG ATGGAGCAGG CCCACAGTGG GTGTGAGGGT 
TTGCTGCGAC AGCGGGATGA AGCTCAGCAG GAGGCGGAGA AACTGAGGAA CAGCTGTAAG 
GACCTGGAAC GCACTCTGAG CACCAGAGAA CGAGCCCACC GGCACCGAGT CAAAGGACTG 
GAGGAGCAGG TGTCCACTTT GAAGGAGCAG CTGCAGCAGG AGATGAAACG GCGGCAGCCT 
CCTCTTCCGT CCACGTTTAC GTCCACTGGG ATCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

crocc ( XM_020701420.1 ) cDNA ORF clone, Oryzias latipes(Japanese medaka) -> XP_020557079.1 Oryzias latipes ciliary rootlet coiled-coil, rootletin (crocc), transcript variant X3, mRNA.

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CloneID	OJa25266
Clone ID Related Accession (Same CDS sequence)	XM_020701420.1 , XM_020701420.2
Accession Version	XM_020701420.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6033bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1491408000000
Organism	Oryzias latipes(Japanese medaka)
Product	rootletin isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_004095157.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Oryzias latipes Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCGCTG CGGAAAACTC CGACAGCGAC CCCAGCAAGC TGGAGTCAGT GATCCGGAGG 
CTGGAGGAAA GTGTTCTGTC TGAGGAGAAG AGGTTGACGG TTCGAGGCCC TTCACCGGAT 
GACCCCCCTG CGTGTCTGCC TGCCAGAGTG CGAGAGATCG TCACGAAGAA CCTCAGTGAC 
AGCACAGGGG AGATGTCCTC GCTCATGTCC CTCCAGGAGG AGAACCGGGT GCTGCAGGGC 
GAGCTGGCCC GGCTGGAGGA CCTGCTGGCG CACAGCAGGG CGGATCGGGA CGAGCTCGCC 
ATCAAGTACG GGGCAATCAG TGAACGGCTG GAGCAGGCTC TACGCTCAGA AACGGGGGAG 
GGAGAGGCCG ACTCACAGGC GTCCCGCAGC CTGGCTCAGC AGAATGTGGA ATTGCGCCGA 
CGGCTGGACG AGGAACAGGC TGCATACAGG CGCAAACTCA CCGCCTACCA AGAAGGCCAG 
CAGAGGCAGG CGCAGCTGGT GCAGAAGCTT CAGGCCAAGG TGCTTCAGTA CAAAAAGAGA 
TGTGGGGATC TGGAGCAGAA GCTGCAGGAG AAGTCCTCAG AGTTAGAGAA ATGCAGTGAG 
CGCAGTGAAA CGTCTAACGG TCGCCATCAC GAGGACTCGA GCAGCAGTTT GGAGGAGGCT 
TTACTACACC TGGAGGAGGA GCAACAGAGG AGCAGCAGTT TATCTGCAGT GAACGCCATG 
CTGAGAGAAC AGTTGGAGCA GGCCGGCTTG GCCAACGAGG CTCTGAGCCG GGACATCCGG 
AGGCTCACCG CTGATTGGAC GAAAGCCAGA GAGGAGCTGG AACAAAAGGA GTCGGACTGG 
AGGAGAGAGG AGGAGTCTTT CCACAGCTAC TTCAGCAGTG AACACGGTCG ACTGCTGACC 
TTTTGGCGAC AGGTGGTGGG GTTCAGGAGG CACGTCTGTG AGATGAAGAG CAGCGCTGAG 
AGGGACCTGT CAGACATGCG GGGCGAGCTG GTTCGGATGT CCCACTTGGT CCAGGTGTCC 
TGCGCGGGTC TGTCCGCCGT GCTGCGCAGT CAGGAAGCAG GAACGGCTCT AGCCCTGGAG 
CAGGAGAGAG CTCTGAGGGA TCAGCTGGAG CAGCAGCTGA GAGGTCAAGT CAAAGAGACG 
ATGAACCTTC AGAGCAGAGC CGATGCAGAG AGAAGTGAGC TGAGCCTCAG GCTTTCAGAT 
GCGGCTCAAG AAAATGAAAG ACTAAAAAGT CAAATGGAAG AAAGAGACCG TGAGATCGAC 
AATCTGAGCA GGAGGCTTGA GAGCCGCAGC GATGAGACGG ACTCCCAGCT GCTGAGGGCT 
CATTGCAACG CTCTGTTGGA CACGCTGAAG GACATCGCTC AGACGGTTCT GTCAGACGGA 
GAGTCGTCTT CAGAGGCCGA TCCGGACAAC TCGGCGGTCG TGCTGCAGGC GCTGATCCGT 
GACTCCTCCC CTCAACGCTC CTCCCAAACC AGCTCCTCGT TGTCCCCTCT CCCGGAAGCG 
GCGCTGTCTG CGCTGCGTTC GGTGGTCACA AACATGATGC TGCAGCTGCA GGATGTTCGA 
GGTCGTCTGC TCTCTGCACA GTCCTCTGCG CAGAAGTTTC GCAAGCAGCT GTCAGAAATC 
GACTCTGCTA AAAGAGACGC AGACCAACGA AACCAAGCTC TGCAGCGAGA CAAGGACGCT 
GCTCTCAGAG AGCGGGAAAC TGCCCAGAGG GAGAAGGAGC AACTGAAGCA AGAGAGAGAC 
AAGCTGGCCA GTGAGAAGGC CAACCTGGAG AAGGCTGTTC AGGCGCTGCA CAGCAGCAAC 
CAGATGCTGC AGATGGACTA CGAGAAGCTC CAGCTGGCTG TGGCTTCTGC ACAGCGAGAG 
CGAGACCGCG AGAGGGAGGA GAAGGAGGCT GCCATCCATG ACGGGGAGCG CTGGAAGACG 
GAGATTCAGA GGATACAGAA GCAGAGGGAC CAGAGTGAAA GTCGAGCCTC GGCTCAGCGG 
GGAGAACTGT CTGCAGTGTT ACAAACTCAC CAGCAGGGGG AGGTGGAGAG GCAGCTGCTG 
GAACAGGAGA GTGCCCACCT TTCAGAGGCC CTTGCTCGGG CTGCAAGCAC CAACAAAGAG 
CTGTCGCTGC ATCTGAGCAA GCTGCAGTAT GAAGATGCAG CTCTCAGAGA CTCTCTCACA 
AAGATGGGAA ACCTGAATGA GGGTCTGGTC CAGGACAAGG TGGACCTTAA CAAGTATATT 
CTGCAGCTTG AGGAGGAGAA GGCCGTCCTA CAGGCTCAGA AACGGGAGGC GGAGCAGGAG 
AAACTGTCCA TCAGGGACGA GCTGGTCCGG CAGGAGCAGA ACCGACTGGA GTTGGAGTCT 
TCCCGCGTCA CACTGCAGCA GTCTCTGCAG GACACCGAGT TCAGTCGGGT GGGCGTGGAG 
GCAGAGCTGC AGAACCTCAG GGCTGAACGA CTGAAGCTAC AGGAAAAGGT CGCCCAGTTG 
TGTGGTGAGG TGGCCTCTCT AGATTCAGAG TTGAGTCTCG CCAAGGGGGA GGGCCAGAGG 
AGCGAAATGG CCTTGGAGGA AGCCGGCCGC AGTCGAGCAG AACTGACCAA AGACAAAGCA 
GCTCTAGTGG TTCAGCTGAC GGCATCTGAG AGAGAAAACG CAATGCTAGC CGAGGAACTG 
ACAGCTTTCA GGTCGGAGCG GGAGACCCTG GAAACGAGTC TCTTTGATGC GCAGCAGCAG 
CTTTTGCAGG CAGAGTCTTG CAGGGAACAG CTGGAAGCAG AAAACCAAAA CCTCCGCGTT 
CGCTGTGAAA CTGCCACAGT GGAGCTCAGG CGTTTGCGCT CAGATGCTGA GAATCAGCTG 
GCCCAGGCTG AGAGAGACAA ACAAGCGCTG ACTCAAGCTC TCAGCGCCGC TCAGCTGGAG 
GCCCAGCAGG CTTCACGCAA GGCCACCTCC GAGCATCAGG AGGAGGTGGA GAGACTGGCC 
TTAGAGAAGG AAGTCGTGCG GCACAGCTTG CAGATGGAGC ACGAGACGGC TGTCCGGAAA 
CTCCGACAAG AAGTCGAAGA TCAGCTCCAG AAAGCCAGGA GAGAAAAAGA GGAGCTGCAG 
GACGGACTGA GGGCTCTGCA GCACGAGCGA GATCAGAGCC TGCTGCAGGC GGAAACGGAG 
AAACAACAGG CCCTTTCACT GAAGGAGGCA GAGAAAACGG TTTTGTTGGA GAAGGTGTCC 
GGTCTGCAGG CTGATCTGTC AGCGCTGAGC CTGGAGGCTG AGCGGACAGC CAGAGAAGCT 
GCCCAGTACA AAGAGCAGGA GCAGATCAGA GTTGGGGCTC TAACCAGCGA ACTGCAAGAA 
CTTCGGTCAC AGATGGAAGA TGCAGCGGCC GGTCATGAAA GGGACATCCT GAGTCTGAAC 
GAAACCTGTG CAGACCTCCG TTCTCGTGCT GATGTTGCCC TCAAAGAGCT GGAGCAGTGC 
AGAGCTTCTC TCTCTGCTGC TGAGGAGAAT CGAGAGCAGC TGAGGCGGGA CTTCCTGGAG 
ACGGAGCGCA GACTCACCCA AACTCAGGAA GCGTCAGAAA ACCACCGGAG GGAAGGAGCC 
GAGCTGCGGC GCAGCCTCTG TGATGTCACC AGGGAGAGAG ATGCTCTCAG CCAATCAAAT 
GCTCAGCTGA GGGAAGCTCT CCGAAGCGCA GAAACGGAGA GAATAAGCGT GAAACGGCAG 
TGTGAGGAGA AGGAGCAGCG GCTGGTTGTG CTGGAGGAGA GCCTGTCCTC CTCGCAGAAG 
GAGGTGGCAG AGCTGCGGAG TTGCCTGAGG GAGGTGGAAA GGTCCCGCCT TGAGGCTCGC 
CGTGAGCTTC AGGAGCTTCG GAGACAGCTG AAGGTTCTGG ATGCAGACAG AGAGCAGAAA 
GGGAGGGAGC TGGCGGAGCT GCAAACCCGC TTGTCGTTGG AGGAGCAAAG AGAGGAGGAA 
AGAGGAAAGG AGGTTTTCAT TTTAAAACAG AAGCTCGCTG AAACCCAAAC GGCCAGAGAG 
TCCCTCAAGA GAGAGCTCTC CATGACACAG AAGCGTCTGG TGGAGTCCGA GTCCGGCTGG 
CGCAGCTGTG AGCGGGAGCT GACGGCACAG CTGCAGGAGG CGCGAGGCTG CGAGAAGAAG 
CTCCAGGATG AGGCCAAGAA CCTGTCGGTG CGCGCGCAAG CGGCGCAGGA ATCCGCCGCT 
CAGTGCAGCC TGCAGCTCAG CGAGGCGCAG GGGAGACTGA CCGCCACCGA AGCTGAGCTG 
ACGCGGGCGG AGGCCTCCAA GAGGGATCTG GAGTTTCGCC TCAGCAGCCT CCAGTCGGCG 
CTGACGCGAA CGCTGGGCAT CGGGGCAAGC GGCAGGGCGA GCAGGGGGAG GAGTCCAGGC 
GGGAGCTCTG CATCACCTGG CAGTATCTCC CGTCACCACA GCATCTCACC TTTGCGCCCT 
TCGCTGTCGC CCCCTAAAGA ATTCAGAGGA AGCACGCCTG ATAACACCAT GGGATCAGGA 
GCTGTGTCTC CTGAAGGAGG CGGTACTCCT TTGCCTCCCG CTCAGCCAGA GCTGGATCCC 
GACACGCTGC GCAGTGGCCT CAGAGACTTC CTGCAGGAGC TGCGAGACGC ACACAGACAA 
AGGGATGATG CCCAATGCCA GCTGATGACT CTGCAGCGGG AGCTGGAGGA GCAGAAGCTG 
GAGCGAGAAT GTGCCGAGAA CCGCTCCACT CAGCTGCAAA ACAGCCTGAA GGAATGCCAA 
GAAGGGAAGC GTGGACTTGA CGACCGTCTG ACCAGCACTC AGATTCTCCT GCAGCAGCAG 
GAGGATGCGG TGAAGAGAGT GGACAGAGAG AGGAGAGCTC TTGCCGACAG GGTGAGGGAG 
TTGGAGCGCT CGCTGCACAC CGCAGAGATG GAGAAGAAGC ACACTCAGGA GCAGTTGAAT 
AAGCAGCGGG CCACTGAGGC ACGCCTGGAG GCGGAGAGGA AGCGCCTGCG GGAGGCTCTG 
GAGGCCGCCG AAGCTCGAGC CACCCGGGTG GAGCTGGGGA GGCGCAGCCT GGAAGGGGAG 
CTGCAGAGGC TCAAACTCGG TCTGGGAGAC CGGGAGGCTG AAAGCCAGGC CTCACAGGAG 
CGCCACGACT GTTTGCTCAG ACAGGTGGCG GAAGGAGAAG CACAAGTCTC TCTGCTGCAG 
ACAGAGGTGG ACAGACTGAG TCAAGCTCTG CTGAAAGCTC AGGAAGGGGA AGCTGCTCTG 
AAGCAGAAGG TCACGACTCT CAGCCAGAGC CTCCAGGAGG CTGCAGCTGA TCACAGCAGC 
ACGCAGACTC GCCTCACTGC TCTGCAGAAA ACACTGAGCT TGGTGGAACA GGACAAAAGA 
CTTCTGCAGG AGAAGATGGA TGAAGCCCGC ACTTCTGTAA CTGAAGCAAA GAGAAGCATG 
TTTGCTCTCA CTGAGCGCGT GCAGAGCCTC CAGGGTGAGC TGACCCAGAG TGAACTGCTG 
AGAGAGGAGC TGGAGGCGGA GCTTAAGAAC ACACAGGAGG CACTCCGACA GCGCTCAGGA 
AGTTTGGCAG AGACTCAGCA CAGCATCCAG AAAGCTCAGA CGGAGCGGGC AGCTGTGGAG 
GAGCGTCTGC TCGCACTGCA AAGAGCTGTG GCTCTGCTGG AGACAGAGAA AAAAGATGCA 
GAGAGGCAAG CTGCAAGGCT GGAGAAGGAC AAGGATGCAC TGAGGAACAC GTTGGATAAG 
GTTGAAAGGC AGAAACTGAA GTGTGAGGAG GGCAGCATGC GGCTCTCAGC AGAGAAGACC 
CGGCTGGACC GCTCCCTGAA CGCAGTGGAG CAGGAGCTGC AGGAGGCGCA GCAGCAGATG 
CTGATGCTGC AGACTCAGCT GGCTGAGATG GAGCAGGCCC ACAGTGGGTG TGAGGGTTTG 
CTGCGACAGC GGGATGAAGC TCAGCAGGAG GCGGAGAAAC TGAGGAACAG CTGTAAGGAC 
CTGGAACGCA CTCTGAGCAC CAGAGAACGA GCCCACCGGC ACCGAGTCAA AGGACTGGAG 
GAGCAGGTGT CCACTTTGAA GGAGCAGCTG CAGCAGGAGA TGAAACGGCG GCAGCCTCCT 
CTTCCGTCCA CGTTTACGTC CACTGGGATC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_020557079.1
CDS	189..6221
Translation	MSAAENSDSDPSKLESVIRRLEESVLSEEKRLTVRGPSPDDPPACLPARVREIVTKNLSDSTGEMSSLMSLQEENRVLQGELARLEDLLAHSRADRDELAIKYGAISERLEQALRSETGEGEADSQASRSLAQQNVELRRRLDEEQAAYRRKLTAYQEGQQRQAQLVQKLQAKVLQYKKRCGDLEQKLQEKSSELEKCSERSETSNGRHHEDSSSSLEEALLHLEEEQQRSSSLSAVNAMLREQLEQAGLANEALSRDIRRLTADWTKAREELEQKESDWRREEESFHSYFSSEHGRLLTFWRQVVGFRRHVCEMKSSAERDLSDMRGELVRMSHLVQVSCAGLSAVLRSQEAGTALALEQERALRDQLEQQLRGQVKETMNLQSRADAERSELSLRLSDAAQENERLKSQMEERDREIDNLSRRLESRSDETDSQLLRAHCNALLDTLKDIAQTVLSDGESSSEADPDNSAVVLQALIRDSSPQRSSQTSSSLSPLPEAALSALRSVVTNMMLQLQDVRGRLLSAQSSAQKFRKQLSEIDSAKRDADQRNQALQRDKDAALRERETAQREKEQLKQERDKLASEKANLEKAVQALHSSNQMLQMDYEKLQLAVASAQRERDREREEKEAAIHDGERWKTEIQRIQKQRDQSESRASAQRGELSAVLQTHQQGEVERQLLEQESAHLSEALARAASTNKELSLHLSKLQYEDAALRDSLTKMGNLNEGLVQDKVDLNKYILQLEEEKAVLQAQKREAEQEKLSIRDELVRQEQNRLELESSRVTLQQSLQDTEFSRVGVEAELQNLRAERLKLQEKVAQLCGEVASLDSELSLAKGEGQRSEMALEEAGRSRAELTKDKAALVVQLTASERENAMLAEELTAFRSERETLETSLFDAQQQLLQAESCREQLEAENQNLRVRCETATVELRRLRSDAENQLAQAERDKQALTQALSAAQLEAQQASRKATSEHQEEVERLALEKEVVRHSLQMEHETAVRKLRQEVEDQLQKARREKEELQDGLRALQHERDQSLLQAETEKQQALSLKEAEKTVLLEKVSGLQADLSALSLEAERTAREAAQYKEQEQIRVGALTSELQELRSQMEDAAAGHERDILSLNETCADLRSRADVALKELEQCRASLSAAEENREQLRRDFLETERRLTQTQEASENHRREGAELRRSLCDVTRERDALSQSNAQLREALRSAETERISVKRQCEEKEQRLVVLEESLSSSQKEVAELRSCLREVERSRLEARRELQELRRQLKVLDADREQKGRELAELQTRLSLEEQREEERGKEVFILKQKLAETQTARESLKRELSMTQKRLVESESGWRSCERELTAQLQEARGCEKKLQDEAKNLSVRAQAAQESAAQCSLQLSEAQGRLTATEAELTRAEASKRDLEFRLSSLQSALTRTLGIGASGRASRGRSPGGSSASPGSISRHHSISPLRPSLSPPKEFRGSTPDNTMGSGAVSPEGGGTPLPPAQPELDPDTLRSGLRDFLQELRDAHRQRDDAQCQLMTLQRELEEQKLERECAENRSTQLQNSLKECQEGKRGLDDRLTSTQILLQQQEDAVKRVDRERRALADRVRELERSLHTAEMEKKHTQEQLNKQRATEARLEAERKRLREALEAAEARATRVELGRRSLEGELQRLKLGLGDREAESQASQERHDCLLRQVAEGEAQVSLLQTEVDRLSQALLKAQEGEAALKQKVTTLSQSLQEAAADHSSTQTRLTALQKTLSLVEQDKRLLQEKMDEARTSVTEAKRSMFALTERVQSLQGELTQSELLREELEAELKNTQEALRQRSGSLAETQHSIQKAQTERAAVEERLLALQRAVALLETEKKDAERQAARLEKDKDALRNTLDKVERQKLKCEEGSMRLSAEKTRLDRSLNAVEQELQEAQQQMLMLQTQLAEMEQAHSGCEGLLRQRDEAQQEAEKLRNSCKDLERTLSTRERAHRHRVKGLEEQVSTLKEQLQQEMKRRQPPLPSTFTSTGI

Target ORF information:

RefSeq Version	XM_020701420.1
Organism	Oryzias latipes(Japanese medaka)
Definition	Oryzias latipes ciliary rootlet coiled-coil, rootletin (crocc), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_020701420.1

ORF Insert Sequence:

ATGAGCGCTG CGGAAAACTC CGACAGCGAC CCCAGCAAGC TGGAGTCAGT GATCCGGAGG 
CTGGAGGAAA GTGTTCTGTC TGAGGAGAAG AGGTTGACGG TTCGAGGCCC TTCACCGGAT 
GACCCCCCTG CGTGTCTGCC TGCCAGAGTG CGAGAGATCG TCACGAAGAA CCTCAGTGAC 
AGCACAGGGG AGATGTCCTC GCTCATGTCC CTCCAGGAGG AGAACCGGGT GCTGCAGGGC 
GAGCTGGCCC GGCTGGAGGA CCTGCTGGCG CACAGCAGGG CGGATCGGGA CGAGCTCGCC 
ATCAAGTACG GGGCAATCAG TGAACGGCTG GAGCAGGCTC TACGCTCAGA AACGGGGGAG 
GGAGAGGCCG ACTCACAGGC GTCCCGCAGC CTGGCTCAGC AGAATGTGGA ATTGCGCCGA 
CGGCTGGACG AGGAACAGGC TGCATACAGG CGCAAACTCA CCGCCTACCA AGAAGGCCAG 
CAGAGGCAGG CGCAGCTGGT GCAGAAGCTT CAGGCCAAGG TGCTTCAGTA CAAAAAGAGA 
TGTGGGGATC TGGAGCAGAA GCTGCAGGAG AAGTCCTCAG AGTTAGAGAA ATGCAGTGAG 
CGCAGTGAAA CGTCTAACGG TCGCCATCAC GAGGACTCGA GCAGCAGTTT GGAGGAGGCT 
TTACTACACC TGGAGGAGGA GCAACAGAGG AGCAGCAGTT TATCTGCAGT GAACGCCATG 
CTGAGAGAAC AGTTGGAGCA GGCCGGCTTG GCCAACGAGG CTCTGAGCCG GGACATCCGG 
AGGCTCACCG CTGATTGGAC GAAAGCCAGA GAGGAGCTGG AACAAAAGGA GTCGGACTGG 
AGGAGAGAGG AGGAGTCTTT CCACAGCTAC TTCAGCAGTG AACACGGTCG ACTGCTGACC 
TTTTGGCGAC AGGTGGTGGG GTTCAGGAGG CACGTCTGTG AGATGAAGAG CAGCGCTGAG 
AGGGACCTGT CAGACATGCG GGGCGAGCTG GTTCGGATGT CCCACTTGGT CCAGGTGTCC 
TGCGCGGGTC TGTCCGCCGT GCTGCGCAGT CAGGAAGCAG GAACGGCTCT AGCCCTGGAG 
CAGGAGAGAG CTCTGAGGGA TCAGCTGGAG CAGCAGCTGA GAGGTCAAGT CAAAGAGACG 
ATGAACCTTC AGAGCAGAGC CGATGCAGAG AGAAGTGAGC TGAGCCTCAG GCTTTCAGAT 
GCGGCTCAAG AAAATGAAAG ACTAAAAAGT CAAATGGAAG AAAGAGACCG TGAGATCGAC 
AATCTGAGCA GGAGGCTTGA GAGCCGCAGC GATGAGACGG ACTCCCAGCT GCTGAGGGCT 
CATTGCAACG CTCTGTTGGA CACGCTGAAG GACATCGCTC AGACGGTTCT GTCAGACGGA 
GAGTCGTCTT CAGAGGCCGA TCCGGACAAC TCGGCGGTCG TGCTGCAGGC GCTGATCCGT 
GACTCCTCCC CTCAACGCTC CTCCCAAACC AGCTCCTCGT TGTCCCCTCT CCCGGAAGCG 
GCGCTGTCTG CGCTGCGTTC GGTGGTCACA AACATGATGC TGCAGCTGCA GGATGTTCGA 
GGTCGTCTGC TCTCTGCACA GTCCTCTGCG CAGAAGTTTC GCAAGCAGCT GTCAGAAATC 
GACTCTGCTA AAAGAGACGC AGACCAACGA AACCAAGCTC TGCAGCGAGA CAAGGACGCT 
GCTCTCAGAG AGCGGGAAAC TGCCCAGAGG GAGAAGGAGC AACTGAAGCA AGAGAGAGAC 
AAGCTGGCCA GTGAGAAGGC CAACCTGGAG AAGGCTGTTC AGGCGCTGCA CAGCAGCAAC 
CAGATGCTGC AGATGGACTA CGAGAAGCTC CAGCTGGCTG TGGCTTCTGC ACAGCGAGAG 
CGAGACCGCG AGAGGGAGGA GAAGGAGGCT GCCATCCATG ACGGGGAGCG CTGGAAGACG 
GAGATTCAGA GGATACAGAA GCAGAGGGAC CAGAGTGAAA GTCGAGCCTC GGCTCAGCGG 
GGAGAACTGT CTGCAGTGTT ACAAACTCAC CAGCAGGGGG AGGTGGAGAG GCAGCTGCTG 
GAACAGGAGA GTGCCCACCT TTCAGAGGCC CTTGCTCGGG CTGCAAGCAC CAACAAAGAG 
CTGTCGCTGC ATCTGAGCAA GCTGCAGTAT GAAGATGCAG CTCTCAGAGA CTCTCTCACA 
AAGATGGGAA ACCTGAATGA GGGTCTGGTC CAGGACAAGG TGGACCTTAA CAAGTATATT 
CTGCAGCTTG AGGAGGAGAA GGCCGTCCTA CAGGCTCAGA AACGGGAGGC GGAGCAGGAG 
AAACTGTCCA TCAGGGACGA GCTGGTCCGG CAGGAGCAGA ACCGACTGGA GTTGGAGTCT 
TCCCGCGTCA CACTGCAGCA GTCTCTGCAG GACACCGAGT TCAGTCGGGT GGGCGTGGAG 
GCAGAGCTGC AGAACCTCAG GGCTGAACGA CTGAAGCTAC AGGAAAAGGT CGCCCAGTTG 
TGTGGTGAGG TGGCCTCTCT AGATTCAGAG TTGAGTCTCG CCAAGGGGGA GGGCCAGAGG 
AGCGAAATGG CCTTGGAGGA AGCCGGCCGC AGTCGAGCAG AACTGACCAA AGACAAAGCA 
GCTCTAGTGG TTCAGCTGAC GGCATCTGAG AGAGAAAACG CAATGCTAGC CGAGGAACTG 
ACAGCTTTCA GGTCGGAGCG GGAGACCCTG GAAACGAGTC TCTTTGATGC GCAGCAGCAG 
CTTTTGCAGG CAGAGTCTTG CAGGGAACAG CTGGAAGCAG AAAACCAAAA CCTCCGCGTT 
CGCTGTGAAA CTGCCACAGT GGAGCTCAGG CGTTTGCGCT CAGATGCTGA GAATCAGCTG 
GCCCAGGCTG AGAGAGACAA ACAAGCGCTG ACTCAAGCTC TCAGCGCCGC TCAGCTGGAG 
GCCCAGCAGG CTTCACGCAA GGCCACCTCC GAGCATCAGG AGGAGGTGGA GAGACTGGCC 
TTAGAGAAGG AAGTCGTGCG GCACAGCTTG CAGATGGAGC ACGAGACGGC TGTCCGGAAA 
CTCCGACAAG AAGTCGAAGA TCAGCTCCAG AAAGCCAGGA GAGAAAAAGA GGAGCTGCAG 
GACGGACTGA GGGCTCTGCA GCACGAGCGA GATCAGAGCC TGCTGCAGGC GGAAACGGAG 
AAACAACAGG CCCTTTCACT GAAGGAGGCA GAGAAAACGG TTTTGTTGGA GAAGGTGTCC 
GGTCTGCAGG CTGATCTGTC AGCGCTGAGC CTGGAGGCTG AGCGGACAGC CAGAGAAGCT 
GCCCAGTACA AAGAGCAGGA GCAGATCAGA GTTGGGGCTC TAACCAGCGA ACTGCAAGAA 
CTTCGGTCAC AGATGGAAGA TGCAGCGGCC GGTCATGAAA GGGACATCCT GAGTCTGAAC 
GAAACCTGTG CAGACCTCCG TTCTCGTGCT GATGTTGCCC TCAAAGAGCT GGAGCAGTGC 
AGAGCTTCTC TCTCTGCTGC TGAGGAGAAT CGAGAGCAGC TGAGGCGGGA CTTCCTGGAG 
ACGGAGCGCA GACTCACCCA AACTCAGGAA GCGTCAGAAA ACCACCGGAG GGAAGGAGCC 
GAGCTGCGGC GCAGCCTCTG TGATGTCACC AGGGAGAGAG ATGCTCTCAG CCAATCAAAT 
GCTCAGCTGA GGGAAGCTCT CCGAAGCGCA GAAACGGAGA GAATAAGCGT GAAACGGCAG 
TGTGAGGAGA AGGAGCAGCG GCTGGTTGTG CTGGAGGAGA GCCTGTCCTC CTCGCAGAAG 
GAGGTGGCAG AGCTGCGGAG TTGCCTGAGG GAGGTGGAAA GGTCCCGCCT TGAGGCTCGC 
CGTGAGCTTC AGGAGCTTCG GAGACAGCTG AAGGTTCTGG ATGCAGACAG AGAGCAGAAA 
GGGAGGGAGC TGGCGGAGCT GCAAACCCGC TTGTCGTTGG AGGAGCAAAG AGAGGAGGAA 
AGAGGAAAGG AGGTTTTCAT TTTAAAACAG AAGCTCGCTG AAACCCAAAC GGCCAGAGAG 
TCCCTCAAGA GAGAGCTCTC CATGACACAG AAGCGTCTGG TGGAGTCCGA GTCCGGCTGG 
CGCAGCTGTG AGCGGGAGCT GACGGCACAG CTGCAGGAGG CGCGAGGCTG CGAGAAGAAG 
CTCCAGGATG AGGCCAAGAA CCTGTCGGTG CGCGCGCAAG CGGCGCAGGA ATCCGCCGCT 
CAGTGCAGCC TGCAGCTCAG CGAGGCGCAG GGGAGACTGA CCGCCACCGA AGCTGAGCTG 
ACGCGGGCGG AGGCCTCCAA GAGGGATCTG GAGTTTCGCC TCAGCAGCCT CCAGTCGGCG 
CTGACGCGAA CGCTGGGCAT CGGGGCAAGC GGCAGGGCGA GCAGGGGGAG GAGTCCAGGC 
GGGAGCTCTG CATCACCTGG CAGTATCTCC CGTCACCACA GCATCTCACC TTTGCGCCCT 
TCGCTGTCGC CCCCTAAAGA ATTCAGAGGA AGCACGCCTG ATAACACCAT GGGATCAGGA 
GCTGTGTCTC CTGAAGGAGG CGGTACTCCT TTGCCTCCCG CTCAGCCAGA GCTGGATCCC 
GACACGCTGC GCAGTGGCCT CAGAGACTTC CTGCAGGAGC TGCGAGACGC ACACAGACAA 
AGGGATGATG CCCAATGCCA GCTGATGACT CTGCAGCGGG AGCTGGAGGA GCAGAAGCTG 
GAGCGAGAAT GTGCCGAGAA CCGCTCCACT CAGCTGCAAA ACAGCCTGAA GGAATGCCAA 
GAAGGGAAGC GTGGACTTGA CGACCGTCTG ACCAGCACTC AGATTCTCCT GCAGCAGCAG 
GAGGATGCGG TGAAGAGAGT GGACAGAGAG AGGAGAGCTC TTGCCGACAG GGTGAGGGAG 
TTGGAGCGCT CGCTGCACAC CGCAGAGATG GAGAAGAAGC ACACTCAGGA GCAGTTGAAT 
AAGCAGCGGG CCACTGAGGC ACGCCTGGAG GCGGAGAGGA AGCGCCTGCG GGAGGCTCTG 
GAGGCCGCCG AAGCTCGAGC CACCCGGGTG GAGCTGGGGA GGCGCAGCCT GGAAGGGGAG 
CTGCAGAGGC TCAAACTCGG TCTGGGAGAC CGGGAGGCTG AAAGCCAGGC CTCACAGGAG 
CGCCACGACT GTTTGCTCAG ACAGGTGGCG GAAGGAGAAG CACAAGTCTC TCTGCTGCAG 
ACAGAGGTGG ACAGACTGAG TCAAGCTCTG CTGAAAGCTC AGGAAGGGGA AGCTGCTCTG 
AAGCAGAAGG TCACGACTCT CAGCCAGAGC CTCCAGGAGG CTGCAGCTGA TCACAGCAGC 
ACGCAGACTC GCCTCACTGC TCTGCAGAAA ACACTGAGCT TGGTGGAACA GGACAAAAGA 
CTTCTGCAGG AGAAGATGGA TGAAGCCCGC ACTTCTGTAA CTGAAGCAAA GAGAAGCATG 
TTTGCTCTCA CTGAGCGCGT GCAGAGCCTC CAGGGTGAGC TGACCCAGAG TGAACTGCTG 
AGAGAGGAGC TGGAGGCGGA GCTTAAGAAC ACACAGGAGG CACTCCGACA GCGCTCAGGA 
AGTTTGGCAG AGACTCAGCA CAGCATCCAG AAAGCTCAGA CGGAGCGGGC AGCTGTGGAG 
GAGCGTCTGC TCGCACTGCA AAGAGCTGTG GCTCTGCTGG AGACAGAGAA AAAAGATGCA 
GAGAGGCAAG CTGCAAGGCT GGAGAAGGAC AAGGATGCAC TGAGGAACAC GTTGGATAAG 
GTTGAAAGGC AGAAACTGAA GTGTGAGGAG GGCAGCATGC GGCTCTCAGC AGAGAAGACC 
CGGCTGGACC GCTCCCTGAA CGCAGTGGAG CAGGAGCTGC AGGAGGCGCA GCAGCAGATG 
CTGATGCTGC AGACTCAGCT GGCTGAGATG GAGCAGGCCC ACAGTGGGTG TGAGGGTTTG 
CTGCGACAGC GGGATGAAGC TCAGCAGGAG GCGGAGAAAC TGAGGAACAG CTGTAAGGAC 
CTGGAACGCA CTCTGAGCAC CAGAGAACGA GCCCACCGGC ACCGAGTCAA AGGACTGGAG 
GAGCAGGTGT CCACTTTGAA GGAGCAGCTG CAGCAGGAGA TGAAACGGCG GCAGCCTCCT 
CTTCCGTCCA CGTTTACGTC CACTGGGATC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

crocc ( XM_020701420.2 ) cDNA ORF clone, Oryzias latipes(Japanese medaka) -> XP_020557079.1 Oryzias latipes ciliary rootlet coiled-coil, rootletin (crocc), transcript variant X3, mRNA.

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CloneID	OJa25266
Clone ID Related Accession (Same CDS sequence)	XM_020701420.1 , XM_020701420.2
Accession Version	XM_020701420.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6033bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1518537600000
Organism	Oryzias latipes(Japanese medaka)
Product	rootletin isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_019863.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Feb 15, 2018 this sequence version replaced XM_020701420.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Oryzias latipes Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCGCTG CGGAAAACTC CGACAGCGAC CCCAGCAAGC TGGAGTCAGT GATCCGGAGG 
CTGGAGGAAA GTGTTCTGTC TGAGGAGAAG AGGTTGACGG TTCGAGGCCC TTCACCGGAT 
GACCCCCCTG CGTGTCTGCC TGCCAGAGTG CGAGAGATCG TCACGAAGAA CCTCAGTGAC 
AGCACAGGGG AGATGTCCTC GCTCATGTCC CTCCAGGAGG AGAACCGGGT GCTGCAGGGC 
GAGCTGGCCC GGCTGGAGGA CCTGCTGGCG CACAGCAGGG CGGATCGGGA CGAGCTCGCC 
ATCAAGTACG GGGCAATCAG TGAACGGCTG GAGCAGGCTC TACGCTCAGA AACGGGGGAG 
GGAGAGGCCG ACTCACAGGC GTCCCGCAGC CTGGCTCAGC AGAATGTGGA ATTGCGCCGA 
CGGCTGGACG AGGAACAGGC TGCATACAGG CGCAAACTCA CCGCCTACCA AGAAGGCCAG 
CAGAGGCAGG CGCAGCTGGT GCAGAAGCTT CAGGCCAAGG TGCTTCAGTA CAAAAAGAGA 
TGTGGGGATC TGGAGCAGAA GCTGCAGGAG AAGTCCTCAG AGTTAGAGAA ATGCAGTGAG 
CGCAGTGAAA CGTCTAACGG TCGCCATCAC GAGGACTCGA GCAGCAGTTT GGAGGAGGCT 
TTACTACACC TGGAGGAGGA GCAACAGAGG AGCAGCAGTT TATCTGCAGT GAACGCCATG 
CTGAGAGAAC AGTTGGAGCA GGCCGGCTTG GCCAACGAGG CTCTGAGCCG GGACATCCGG 
AGGCTCACCG CTGATTGGAC GAAAGCCAGA GAGGAGCTGG AACAAAAGGA GTCGGACTGG 
AGGAGAGAGG AGGAGTCTTT CCACAGCTAC TTCAGCAGTG AACACGGTCG ACTGCTGACC 
TTTTGGCGAC AGGTGGTGGG GTTCAGGAGG CACGTCTGTG AGATGAAGAG CAGCGCTGAG 
AGGGACCTGT CAGACATGCG GGGCGAGCTG GTTCGGATGT CCCACTTGGT CCAGGTGTCC 
TGCGCGGGTC TGTCCGCCGT GCTGCGCAGT CAGGAAGCAG GAACGGCTCT AGCCCTGGAG 
CAGGAGAGAG CTCTGAGGGA TCAGCTGGAG CAGCAGCTGA GAGGTCAAGT CAAAGAGACG 
ATGAACCTTC AGAGCAGAGC CGATGCAGAG AGAAGTGAGC TGAGCCTCAG GCTTTCAGAT 
GCGGCTCAAG AAAATGAAAG ACTAAAAAGT CAAATGGAAG AAAGAGACCG TGAGATCGAC 
AATCTGAGCA GGAGGCTTGA GAGCCGCAGC GATGAGACGG ACTCCCAGCT GCTGAGGGCT 
CATTGCAACG CTCTGTTGGA CACGCTGAAG GACATCGCTC AGACGGTTCT GTCAGACGGA 
GAGTCGTCTT CAGAGGCCGA TCCGGACAAC TCGGCGGTCG TGCTGCAGGC GCTGATCCGT 
GACTCCTCCC CTCAACGCTC CTCCCAAACC AGCTCCTCGT TGTCCCCTCT CCCGGAAGCG 
GCGCTGTCTG CGCTGCGTTC GGTGGTCACA AACATGATGC TGCAGCTGCA GGATGTTCGA 
GGTCGTCTGC TCTCTGCACA GTCCTCTGCG CAGAAGTTTC GCAAGCAGCT GTCAGAAATC 
GACTCTGCTA AAAGAGACGC AGACCAACGA AACCAAGCTC TGCAGCGAGA CAAGGACGCT 
GCTCTCAGAG AGCGGGAAAC TGCCCAGAGG GAGAAGGAGC AACTGAAGCA AGAGAGAGAC 
AAGCTGGCCA GTGAGAAGGC CAACCTGGAG AAGGCTGTTC AGGCGCTGCA CAGCAGCAAC 
CAGATGCTGC AGATGGACTA CGAGAAGCTC CAGCTGGCTG TGGCTTCTGC ACAGCGAGAG 
CGAGACCGCG AGAGGGAGGA GAAGGAGGCT GCCATCCATG ACGGGGAGCG CTGGAAGACG 
GAGATTCAGA GGATACAGAA GCAGAGGGAC CAGAGTGAAA GTCGAGCCTC GGCTCAGCGG 
GGAGAACTGT CTGCAGTGTT ACAAACTCAC CAGCAGGGGG AGGTGGAGAG GCAGCTGCTG 
GAACAGGAGA GTGCCCACCT TTCAGAGGCC CTTGCTCGGG CTGCAAGCAC CAACAAAGAG 
CTGTCGCTGC ATCTGAGCAA GCTGCAGTAT GAAGATGCAG CTCTCAGAGA CTCTCTCACA 
AAGATGGGAA ACCTGAATGA GGGTCTGGTC CAGGACAAGG TGGACCTTAA CAAGTATATT 
CTGCAGCTTG AGGAGGAGAA GGCCGTCCTA CAGGCTCAGA AACGGGAGGC GGAGCAGGAG 
AAACTGTCCA TCAGGGACGA GCTGGTCCGG CAGGAGCAGA ACCGACTGGA GTTGGAGTCT 
TCCCGCGTCA CACTGCAGCA GTCTCTGCAG GACACCGAGT TCAGTCGGGT GGGCGTGGAG 
GCAGAGCTGC AGAACCTCAG GGCTGAACGA CTGAAGCTAC AGGAAAAGGT CGCCCAGTTG 
TGTGGTGAGG TGGCCTCTCT AGATTCAGAG TTGAGTCTCG CCAAGGGGGA GGGCCAGAGG 
AGCGAAATGG CCTTGGAGGA AGCCGGCCGC AGTCGAGCAG AACTGACCAA AGACAAAGCA 
GCTCTAGTGG TTCAGCTGAC GGCATCTGAG AGAGAAAACG CAATGCTAGC CGAGGAACTG 
ACAGCTTTCA GGTCGGAGCG GGAGACCCTG GAAACGAGTC TCTTTGATGC GCAGCAGCAG 
CTTTTGCAGG CAGAGTCTTG CAGGGAACAG CTGGAAGCAG AAAACCAAAA CCTCCGCGTT 
CGCTGTGAAA CTGCCACAGT GGAGCTCAGG CGTTTGCGCT CAGATGCTGA GAATCAGCTG 
GCCCAGGCTG AGAGAGACAA ACAAGCGCTG ACTCAAGCTC TCAGCGCCGC TCAGCTGGAG 
GCCCAGCAGG CTTCACGCAA GGCCACCTCC GAGCATCAGG AGGAGGTGGA GAGACTGGCC 
TTAGAGAAGG AAGTCGTGCG GCACAGCTTG CAGATGGAGC ACGAGACGGC TGTCCGGAAA 
CTCCGACAAG AAGTCGAAGA TCAGCTCCAG AAAGCCAGGA GAGAAAAAGA GGAGCTGCAG 
GACGGACTGA GGGCTCTGCA GCACGAGCGA GATCAGAGCC TGCTGCAGGC GGAAACGGAG 
AAACAACAGG CCCTTTCACT GAAGGAGGCA GAGAAAACGG TTTTGTTGGA GAAGGTGTCC 
GGTCTGCAGG CTGATCTGTC AGCGCTGAGC CTGGAGGCTG AGCGGACAGC CAGAGAAGCT 
GCCCAGTACA AAGAGCAGGA GCAGATCAGA GTTGGGGCTC TAACCAGCGA ACTGCAAGAA 
CTTCGGTCAC AGATGGAAGA TGCAGCGGCC GGTCATGAAA GGGACATCCT GAGTCTGAAC 
GAAACCTGTG CAGACCTCCG TTCTCGTGCT GATGTTGCCC TCAAAGAGCT GGAGCAGTGC 
AGAGCTTCTC TCTCTGCTGC TGAGGAGAAT CGAGAGCAGC TGAGGCGGGA CTTCCTGGAG 
ACGGAGCGCA GACTCACCCA AACTCAGGAA GCGTCAGAAA ACCACCGGAG GGAAGGAGCC 
GAGCTGCGGC GCAGCCTCTG TGATGTCACC AGGGAGAGAG ATGCTCTCAG CCAATCAAAT 
GCTCAGCTGA GGGAAGCTCT CCGAAGCGCA GAAACGGAGA GAATAAGCGT GAAACGGCAG 
TGTGAGGAGA AGGAGCAGCG GCTGGTTGTG CTGGAGGAGA GCCTGTCCTC CTCGCAGAAG 
GAGGTGGCAG AGCTGCGGAG TTGCCTGAGG GAGGTGGAAA GGTCCCGCCT TGAGGCTCGC 
CGTGAGCTTC AGGAGCTTCG GAGACAGCTG AAGGTTCTGG ATGCAGACAG AGAGCAGAAA 
GGGAGGGAGC TGGCGGAGCT GCAAACCCGC TTGTCGTTGG AGGAGCAAAG AGAGGAGGAA 
AGAGGAAAGG AGGTTTTCAT TTTAAAACAG AAGCTCGCTG AAACCCAAAC GGCCAGAGAG 
TCCCTCAAGA GAGAGCTCTC CATGACACAG AAGCGTCTGG TGGAGTCCGA GTCCGGCTGG 
CGCAGCTGTG AGCGGGAGCT GACGGCACAG CTGCAGGAGG CGCGAGGCTG CGAGAAGAAG 
CTCCAGGATG AGGCCAAGAA CCTGTCGGTG CGCGCGCAAG CGGCGCAGGA ATCCGCCGCT 
CAGTGCAGCC TGCAGCTCAG CGAGGCGCAG GGGAGACTGA CCGCCACCGA AGCTGAGCTG 
ACGCGGGCGG AGGCCTCCAA GAGGGATCTG GAGTTTCGCC TCAGCAGCCT CCAGTCGGCG 
CTGACGCGAA CGCTGGGCAT CGGGGCAAGC GGCAGGGCGA GCAGGGGGAG GAGTCCAGGC 
GGGAGCTCTG CATCACCTGG CAGTATCTCC CGTCACCACA GCATCTCACC TTTGCGCCCT 
TCGCTGTCGC CCCCTAAAGA ATTCAGAGGA AGCACGCCTG ATAACACCAT GGGATCAGGA 
GCTGTGTCTC CTGAAGGAGG CGGTACTCCT TTGCCTCCCG CTCAGCCAGA GCTGGATCCC 
GACACGCTGC GCAGTGGCCT CAGAGACTTC CTGCAGGAGC TGCGAGACGC ACACAGACAA 
AGGGATGATG CCCAATGCCA GCTGATGACT CTGCAGCGGG AGCTGGAGGA GCAGAAGCTG 
GAGCGAGAAT GTGCCGAGAA CCGCTCCACT CAGCTGCAAA ACAGCCTGAA GGAATGCCAA 
GAAGGGAAGC GTGGACTTGA CGACCGTCTG ACCAGCACTC AGATTCTCCT GCAGCAGCAG 
GAGGATGCGG TGAAGAGAGT GGACAGAGAG AGGAGAGCTC TTGCCGACAG GGTGAGGGAG 
TTGGAGCGCT CGCTGCACAC CGCAGAGATG GAGAAGAAGC ACACTCAGGA GCAGTTGAAT 
AAGCAGCGGG CCACTGAGGC ACGCCTGGAG GCGGAGAGGA AGCGCCTGCG GGAGGCTCTG 
GAGGCCGCCG AAGCTCGAGC CACCCGGGTG GAGCTGGGGA GGCGCAGCCT GGAAGGGGAG 
CTGCAGAGGC TCAAACTCGG TCTGGGAGAC CGGGAGGCTG AAAGCCAGGC CTCACAGGAG 
CGCCACGACT GTTTGCTCAG ACAGGTGGCG GAAGGAGAAG CACAAGTCTC TCTGCTGCAG 
ACAGAGGTGG ACAGACTGAG TCAAGCTCTG CTGAAAGCTC AGGAAGGGGA AGCTGCTCTG 
AAGCAGAAGG TCACGACTCT CAGCCAGAGC CTCCAGGAGG CTGCAGCTGA TCACAGCAGC 
ACGCAGACTC GCCTCACTGC TCTGCAGAAA ACACTGAGCT TGGTGGAACA GGACAAAAGA 
CTTCTGCAGG AGAAGATGGA TGAAGCCCGC ACTTCTGTAA CTGAAGCAAA GAGAAGCATG 
TTTGCTCTCA CTGAGCGCGT GCAGAGCCTC CAGGGTGAGC TGACCCAGAG TGAACTGCTG 
AGAGAGGAGC TGGAGGCGGA GCTTAAGAAC ACACAGGAGG CACTCCGACA GCGCTCAGGA 
AGTTTGGCAG AGACTCAGCA CAGCATCCAG AAAGCTCAGA CGGAGCGGGC AGCTGTGGAG 
GAGCGTCTGC TCGCACTGCA AAGAGCTGTG GCTCTGCTGG AGACAGAGAA AAAAGATGCA 
GAGAGGCAAG CTGCAAGGCT GGAGAAGGAC AAGGATGCAC TGAGGAACAC GTTGGATAAG 
GTTGAAAGGC AGAAACTGAA GTGTGAGGAG GGCAGCATGC GGCTCTCAGC AGAGAAGACC 
CGGCTGGACC GCTCCCTGAA CGCAGTGGAG CAGGAGCTGC AGGAGGCGCA GCAGCAGATG 
CTGATGCTGC AGACTCAGCT GGCTGAGATG GAGCAGGCCC ACAGTGGGTG TGAGGGTTTG 
CTGCGACAGC GGGATGAAGC TCAGCAGGAG GCGGAGAAAC TGAGGAACAG CTGTAAGGAC 
CTGGAACGCA CTCTGAGCAC CAGAGAACGA GCCCACCGGC ACCGAGTCAA AGGACTGGAG 
GAGCAGGTGT CCACTTTGAA GGAGCAGCTG CAGCAGGAGA TGAAACGGCG GCAGCCTCCT 
CTTCCGTCCA CGTTTACGTC CACTGGGATC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_020557079.1
CDS	199..6231
Translation	MSAAENSDSDPSKLESVIRRLEESVLSEEKRLTVRGPSPDDPPACLPARVREIVTKNLSDSTGEMSSLMSLQEENRVLQGELARLEDLLAHSRADRDELAIKYGAISERLEQALRSETGEGEADSQASRSLAQQNVELRRRLDEEQAAYRRKLTAYQEGQQRQAQLVQKLQAKVLQYKKRCGDLEQKLQEKSSELEKCSERSETSNGRHHEDSSSSLEEALLHLEEEQQRSSSLSAVNAMLREQLEQAGLANEALSRDIRRLTADWTKAREELEQKESDWRREEESFHSYFSSEHGRLLTFWRQVVGFRRHVCEMKSSAERDLSDMRGELVRMSHLVQVSCAGLSAVLRSQEAGTALALEQERALRDQLEQQLRGQVKETMNLQSRADAERSELSLRLSDAAQENERLKSQMEERDREIDNLSRRLESRSDETDSQLLRAHCNALLDTLKDIAQTVLSDGESSSEADPDNSAVVLQALIRDSSPQRSSQTSSSLSPLPEAALSALRSVVTNMMLQLQDVRGRLLSAQSSAQKFRKQLSEIDSAKRDADQRNQALQRDKDAALRERETAQREKEQLKQERDKLASEKANLEKAVQALHSSNQMLQMDYEKLQLAVASAQRERDREREEKEAAIHDGERWKTEIQRIQKQRDQSESRASAQRGELSAVLQTHQQGEVERQLLEQESAHLSEALARAASTNKELSLHLSKLQYEDAALRDSLTKMGNLNEGLVQDKVDLNKYILQLEEEKAVLQAQKREAEQEKLSIRDELVRQEQNRLELESSRVTLQQSLQDTEFSRVGVEAELQNLRAERLKLQEKVAQLCGEVASLDSELSLAKGEGQRSEMALEEAGRSRAELTKDKAALVVQLTASERENAMLAEELTAFRSERETLETSLFDAQQQLLQAESCREQLEAENQNLRVRCETATVELRRLRSDAENQLAQAERDKQALTQALSAAQLEAQQASRKATSEHQEEVERLALEKEVVRHSLQMEHETAVRKLRQEVEDQLQKARREKEELQDGLRALQHERDQSLLQAETEKQQALSLKEAEKTVLLEKVSGLQADLSALSLEAERTAREAAQYKEQEQIRVGALTSELQELRSQMEDAAAGHERDILSLNETCADLRSRADVALKELEQCRASLSAAEENREQLRRDFLETERRLTQTQEASENHRREGAELRRSLCDVTRERDALSQSNAQLREALRSAETERISVKRQCEEKEQRLVVLEESLSSSQKEVAELRSCLREVERSRLEARRELQELRRQLKVLDADREQKGRELAELQTRLSLEEQREEERGKEVFILKQKLAETQTARESLKRELSMTQKRLVESESGWRSCERELTAQLQEARGCEKKLQDEAKNLSVRAQAAQESAAQCSLQLSEAQGRLTATEAELTRAEASKRDLEFRLSSLQSALTRTLGIGASGRASRGRSPGGSSASPGSISRHHSISPLRPSLSPPKEFRGSTPDNTMGSGAVSPEGGGTPLPPAQPELDPDTLRSGLRDFLQELRDAHRQRDDAQCQLMTLQRELEEQKLERECAENRSTQLQNSLKECQEGKRGLDDRLTSTQILLQQQEDAVKRVDRERRALADRVRELERSLHTAEMEKKHTQEQLNKQRATEARLEAERKRLREALEAAEARATRVELGRRSLEGELQRLKLGLGDREAESQASQERHDCLLRQVAEGEAQVSLLQTEVDRLSQALLKAQEGEAALKQKVTTLSQSLQEAAADHSSTQTRLTALQKTLSLVEQDKRLLQEKMDEARTSVTEAKRSMFALTERVQSLQGELTQSELLREELEAELKNTQEALRQRSGSLAETQHSIQKAQTERAAVEERLLALQRAVALLETEKKDAERQAARLEKDKDALRNTLDKVERQKLKCEEGSMRLSAEKTRLDRSLNAVEQELQEAQQQMLMLQTQLAEMEQAHSGCEGLLRQRDEAQQEAEKLRNSCKDLERTLSTRERAHRHRVKGLEEQVSTLKEQLQQEMKRRQPPLPSTFTSTGI

Target ORF information:

RefSeq Version	XM_020701420.2
Organism	Oryzias latipes(Japanese medaka)
Definition	Oryzias latipes ciliary rootlet coiled-coil, rootletin (crocc), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_020701420.2

ORF Insert Sequence:

ATGAGCGCTG CGGAAAACTC CGACAGCGAC CCCAGCAAGC TGGAGTCAGT GATCCGGAGG 
CTGGAGGAAA GTGTTCTGTC TGAGGAGAAG AGGTTGACGG TTCGAGGCCC TTCACCGGAT 
GACCCCCCTG CGTGTCTGCC TGCCAGAGTG CGAGAGATCG TCACGAAGAA CCTCAGTGAC 
AGCACAGGGG AGATGTCCTC GCTCATGTCC CTCCAGGAGG AGAACCGGGT GCTGCAGGGC 
GAGCTGGCCC GGCTGGAGGA CCTGCTGGCG CACAGCAGGG CGGATCGGGA CGAGCTCGCC 
ATCAAGTACG GGGCAATCAG TGAACGGCTG GAGCAGGCTC TACGCTCAGA AACGGGGGAG 
GGAGAGGCCG ACTCACAGGC GTCCCGCAGC CTGGCTCAGC AGAATGTGGA ATTGCGCCGA 
CGGCTGGACG AGGAACAGGC TGCATACAGG CGCAAACTCA CCGCCTACCA AGAAGGCCAG 
CAGAGGCAGG CGCAGCTGGT GCAGAAGCTT CAGGCCAAGG TGCTTCAGTA CAAAAAGAGA 
TGTGGGGATC TGGAGCAGAA GCTGCAGGAG AAGTCCTCAG AGTTAGAGAA ATGCAGTGAG 
CGCAGTGAAA CGTCTAACGG TCGCCATCAC GAGGACTCGA GCAGCAGTTT GGAGGAGGCT 
TTACTACACC TGGAGGAGGA GCAACAGAGG AGCAGCAGTT TATCTGCAGT GAACGCCATG 
CTGAGAGAAC AGTTGGAGCA GGCCGGCTTG GCCAACGAGG CTCTGAGCCG GGACATCCGG 
AGGCTCACCG CTGATTGGAC GAAAGCCAGA GAGGAGCTGG AACAAAAGGA GTCGGACTGG 
AGGAGAGAGG AGGAGTCTTT CCACAGCTAC TTCAGCAGTG AACACGGTCG ACTGCTGACC 
TTTTGGCGAC AGGTGGTGGG GTTCAGGAGG CACGTCTGTG AGATGAAGAG CAGCGCTGAG 
AGGGACCTGT CAGACATGCG GGGCGAGCTG GTTCGGATGT CCCACTTGGT CCAGGTGTCC 
TGCGCGGGTC TGTCCGCCGT GCTGCGCAGT CAGGAAGCAG GAACGGCTCT AGCCCTGGAG 
CAGGAGAGAG CTCTGAGGGA TCAGCTGGAG CAGCAGCTGA GAGGTCAAGT CAAAGAGACG 
ATGAACCTTC AGAGCAGAGC CGATGCAGAG AGAAGTGAGC TGAGCCTCAG GCTTTCAGAT 
GCGGCTCAAG AAAATGAAAG ACTAAAAAGT CAAATGGAAG AAAGAGACCG TGAGATCGAC 
AATCTGAGCA GGAGGCTTGA GAGCCGCAGC GATGAGACGG ACTCCCAGCT GCTGAGGGCT 
CATTGCAACG CTCTGTTGGA CACGCTGAAG GACATCGCTC AGACGGTTCT GTCAGACGGA 
GAGTCGTCTT CAGAGGCCGA TCCGGACAAC TCGGCGGTCG TGCTGCAGGC GCTGATCCGT 
GACTCCTCCC CTCAACGCTC CTCCCAAACC AGCTCCTCGT TGTCCCCTCT CCCGGAAGCG 
GCGCTGTCTG CGCTGCGTTC GGTGGTCACA AACATGATGC TGCAGCTGCA GGATGTTCGA 
GGTCGTCTGC TCTCTGCACA GTCCTCTGCG CAGAAGTTTC GCAAGCAGCT GTCAGAAATC 
GACTCTGCTA AAAGAGACGC AGACCAACGA AACCAAGCTC TGCAGCGAGA CAAGGACGCT 
GCTCTCAGAG AGCGGGAAAC TGCCCAGAGG GAGAAGGAGC AACTGAAGCA AGAGAGAGAC 
AAGCTGGCCA GTGAGAAGGC CAACCTGGAG AAGGCTGTTC AGGCGCTGCA CAGCAGCAAC 
CAGATGCTGC AGATGGACTA CGAGAAGCTC CAGCTGGCTG TGGCTTCTGC ACAGCGAGAG 
CGAGACCGCG AGAGGGAGGA GAAGGAGGCT GCCATCCATG ACGGGGAGCG CTGGAAGACG 
GAGATTCAGA GGATACAGAA GCAGAGGGAC CAGAGTGAAA GTCGAGCCTC GGCTCAGCGG 
GGAGAACTGT CTGCAGTGTT ACAAACTCAC CAGCAGGGGG AGGTGGAGAG GCAGCTGCTG 
GAACAGGAGA GTGCCCACCT TTCAGAGGCC CTTGCTCGGG CTGCAAGCAC CAACAAAGAG 
CTGTCGCTGC ATCTGAGCAA GCTGCAGTAT GAAGATGCAG CTCTCAGAGA CTCTCTCACA 
AAGATGGGAA ACCTGAATGA GGGTCTGGTC CAGGACAAGG TGGACCTTAA CAAGTATATT 
CTGCAGCTTG AGGAGGAGAA GGCCGTCCTA CAGGCTCAGA AACGGGAGGC GGAGCAGGAG 
AAACTGTCCA TCAGGGACGA GCTGGTCCGG CAGGAGCAGA ACCGACTGGA GTTGGAGTCT 
TCCCGCGTCA CACTGCAGCA GTCTCTGCAG GACACCGAGT TCAGTCGGGT GGGCGTGGAG 
GCAGAGCTGC AGAACCTCAG GGCTGAACGA CTGAAGCTAC AGGAAAAGGT CGCCCAGTTG 
TGTGGTGAGG TGGCCTCTCT AGATTCAGAG TTGAGTCTCG CCAAGGGGGA GGGCCAGAGG 
AGCGAAATGG CCTTGGAGGA AGCCGGCCGC AGTCGAGCAG AACTGACCAA AGACAAAGCA 
GCTCTAGTGG TTCAGCTGAC GGCATCTGAG AGAGAAAACG CAATGCTAGC CGAGGAACTG 
ACAGCTTTCA GGTCGGAGCG GGAGACCCTG GAAACGAGTC TCTTTGATGC GCAGCAGCAG 
CTTTTGCAGG CAGAGTCTTG CAGGGAACAG CTGGAAGCAG AAAACCAAAA CCTCCGCGTT 
CGCTGTGAAA CTGCCACAGT GGAGCTCAGG CGTTTGCGCT CAGATGCTGA GAATCAGCTG 
GCCCAGGCTG AGAGAGACAA ACAAGCGCTG ACTCAAGCTC TCAGCGCCGC TCAGCTGGAG 
GCCCAGCAGG CTTCACGCAA GGCCACCTCC GAGCATCAGG AGGAGGTGGA GAGACTGGCC 
TTAGAGAAGG AAGTCGTGCG GCACAGCTTG CAGATGGAGC ACGAGACGGC TGTCCGGAAA 
CTCCGACAAG AAGTCGAAGA TCAGCTCCAG AAAGCCAGGA GAGAAAAAGA GGAGCTGCAG 
GACGGACTGA GGGCTCTGCA GCACGAGCGA GATCAGAGCC TGCTGCAGGC GGAAACGGAG 
AAACAACAGG CCCTTTCACT GAAGGAGGCA GAGAAAACGG TTTTGTTGGA GAAGGTGTCC 
GGTCTGCAGG CTGATCTGTC AGCGCTGAGC CTGGAGGCTG AGCGGACAGC CAGAGAAGCT 
GCCCAGTACA AAGAGCAGGA GCAGATCAGA GTTGGGGCTC TAACCAGCGA ACTGCAAGAA 
CTTCGGTCAC AGATGGAAGA TGCAGCGGCC GGTCATGAAA GGGACATCCT GAGTCTGAAC 
GAAACCTGTG CAGACCTCCG TTCTCGTGCT GATGTTGCCC TCAAAGAGCT GGAGCAGTGC 
AGAGCTTCTC TCTCTGCTGC TGAGGAGAAT CGAGAGCAGC TGAGGCGGGA CTTCCTGGAG 
ACGGAGCGCA GACTCACCCA AACTCAGGAA GCGTCAGAAA ACCACCGGAG GGAAGGAGCC 
GAGCTGCGGC GCAGCCTCTG TGATGTCACC AGGGAGAGAG ATGCTCTCAG CCAATCAAAT 
GCTCAGCTGA GGGAAGCTCT CCGAAGCGCA GAAACGGAGA GAATAAGCGT GAAACGGCAG 
TGTGAGGAGA AGGAGCAGCG GCTGGTTGTG CTGGAGGAGA GCCTGTCCTC CTCGCAGAAG 
GAGGTGGCAG AGCTGCGGAG TTGCCTGAGG GAGGTGGAAA GGTCCCGCCT TGAGGCTCGC 
CGTGAGCTTC AGGAGCTTCG GAGACAGCTG AAGGTTCTGG ATGCAGACAG AGAGCAGAAA 
GGGAGGGAGC TGGCGGAGCT GCAAACCCGC TTGTCGTTGG AGGAGCAAAG AGAGGAGGAA 
AGAGGAAAGG AGGTTTTCAT TTTAAAACAG AAGCTCGCTG AAACCCAAAC GGCCAGAGAG 
TCCCTCAAGA GAGAGCTCTC CATGACACAG AAGCGTCTGG TGGAGTCCGA GTCCGGCTGG 
CGCAGCTGTG AGCGGGAGCT GACGGCACAG CTGCAGGAGG CGCGAGGCTG CGAGAAGAAG 
CTCCAGGATG AGGCCAAGAA CCTGTCGGTG CGCGCGCAAG CGGCGCAGGA ATCCGCCGCT 
CAGTGCAGCC TGCAGCTCAG CGAGGCGCAG GGGAGACTGA CCGCCACCGA AGCTGAGCTG 
ACGCGGGCGG AGGCCTCCAA GAGGGATCTG GAGTTTCGCC TCAGCAGCCT CCAGTCGGCG 
CTGACGCGAA CGCTGGGCAT CGGGGCAAGC GGCAGGGCGA GCAGGGGGAG GAGTCCAGGC 
GGGAGCTCTG CATCACCTGG CAGTATCTCC CGTCACCACA GCATCTCACC TTTGCGCCCT 
TCGCTGTCGC CCCCTAAAGA ATTCAGAGGA AGCACGCCTG ATAACACCAT GGGATCAGGA 
GCTGTGTCTC CTGAAGGAGG CGGTACTCCT TTGCCTCCCG CTCAGCCAGA GCTGGATCCC 
GACACGCTGC GCAGTGGCCT CAGAGACTTC CTGCAGGAGC TGCGAGACGC ACACAGACAA 
AGGGATGATG CCCAATGCCA GCTGATGACT CTGCAGCGGG AGCTGGAGGA GCAGAAGCTG 
GAGCGAGAAT GTGCCGAGAA CCGCTCCACT CAGCTGCAAA ACAGCCTGAA GGAATGCCAA 
GAAGGGAAGC GTGGACTTGA CGACCGTCTG ACCAGCACTC AGATTCTCCT GCAGCAGCAG 
GAGGATGCGG TGAAGAGAGT GGACAGAGAG AGGAGAGCTC TTGCCGACAG GGTGAGGGAG 
TTGGAGCGCT CGCTGCACAC CGCAGAGATG GAGAAGAAGC ACACTCAGGA GCAGTTGAAT 
AAGCAGCGGG CCACTGAGGC ACGCCTGGAG GCGGAGAGGA AGCGCCTGCG GGAGGCTCTG 
GAGGCCGCCG AAGCTCGAGC CACCCGGGTG GAGCTGGGGA GGCGCAGCCT GGAAGGGGAG 
CTGCAGAGGC TCAAACTCGG TCTGGGAGAC CGGGAGGCTG AAAGCCAGGC CTCACAGGAG 
CGCCACGACT GTTTGCTCAG ACAGGTGGCG GAAGGAGAAG CACAAGTCTC TCTGCTGCAG 
ACAGAGGTGG ACAGACTGAG TCAAGCTCTG CTGAAAGCTC AGGAAGGGGA AGCTGCTCTG 
AAGCAGAAGG TCACGACTCT CAGCCAGAGC CTCCAGGAGG CTGCAGCTGA TCACAGCAGC 
ACGCAGACTC GCCTCACTGC TCTGCAGAAA ACACTGAGCT TGGTGGAACA GGACAAAAGA 
CTTCTGCAGG AGAAGATGGA TGAAGCCCGC ACTTCTGTAA CTGAAGCAAA GAGAAGCATG 
TTTGCTCTCA CTGAGCGCGT GCAGAGCCTC CAGGGTGAGC TGACCCAGAG TGAACTGCTG 
AGAGAGGAGC TGGAGGCGGA GCTTAAGAAC ACACAGGAGG CACTCCGACA GCGCTCAGGA 
AGTTTGGCAG AGACTCAGCA CAGCATCCAG AAAGCTCAGA CGGAGCGGGC AGCTGTGGAG 
GAGCGTCTGC TCGCACTGCA AAGAGCTGTG GCTCTGCTGG AGACAGAGAA AAAAGATGCA 
GAGAGGCAAG CTGCAAGGCT GGAGAAGGAC AAGGATGCAC TGAGGAACAC GTTGGATAAG 
GTTGAAAGGC AGAAACTGAA GTGTGAGGAG GGCAGCATGC GGCTCTCAGC AGAGAAGACC 
CGGCTGGACC GCTCCCTGAA CGCAGTGGAG CAGGAGCTGC AGGAGGCGCA GCAGCAGATG 
CTGATGCTGC AGACTCAGCT GGCTGAGATG GAGCAGGCCC ACAGTGGGTG TGAGGGTTTG 
CTGCGACAGC GGGATGAAGC TCAGCAGGAG GCGGAGAAAC TGAGGAACAG CTGTAAGGAC 
CTGGAACGCA CTCTGAGCAC CAGAGAACGA GCCCACCGGC ACCGAGTCAA AGGACTGGAG 
GAGCAGGTGT CCACTTTGAA GGAGCAGCTG CAGCAGGAGA TGAAACGGCG GCAGCCTCCT 
CTTCCGTCCA CGTTTACGTC CACTGGGATC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

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crocc cDNA ORF clone, Oryzias latipes(Japanese medaka)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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