nrap cDNA ORF clone, Astyanax mexicanus(Mexican tetra)

Gene
Clones
gRNAs

Summary

Gene Symbol	nrap
Entrez Gene ID	103040909
Full Name	nebulin related anchoring protein
Gene Type	protein-coding
Organism	Astyanax mexicanus(Mexican tetra)

mRNA and Protein(s)

mRNA	Protein	Name
XM_007259249.1	XP_007259311.2	nebulin-related-anchoring protein
XM_007259249.2	XP_007259311.3	LOW QUALITY PROTEIN: nebulin-related-anchoring protein

Pathways from BioSystems
Gene Ontology

The following nrap gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the nrap cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OAs124814	XM_007259249.2 Latest version!	Astyanax mexicanus nebulin related anchoring protein (nrap), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OAs40986	XM_007259249.1 Latest version!	Astyanax mexicanus nebulin related anchoring protein (nrap), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00

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Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

nrap ( XM_007259249.2 ) cDNA ORF clone, Astyanax mexicanus(Mexican tetra) -> XP_007259311.3 Astyanax mexicanus nebulin related anchoring protein (nrap), mRNA.

Price & Availability↑

CloneID	OAs124814
Clone ID Related Accession (Same CDS sequence)	XM_007259249.2
Accession Version	XM_007259249.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5520bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1506873600000
Organism	Astyanax mexicanus(Mexican tetra)
Product	LOW QUALITY PROTEIN: nebulin-related-anchoring protein
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_035900.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Oct 3, 2017 this sequence version replaced XM_007259249.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Astyanax mexicanus Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## frameshifts :: corrected 1 indel ##RefSeq-Attributes-END##

ATGAATATTC AAAACTGTGC CAGGTGTGGG TTTGTGGTGT ACCCAGCTGA GAAGATCAAT 
CTCATTAGTC AGAACTGGCA CAAAGCATGC TTCCACTGTG AGGTCTGTAA GATGGTTCTA 
ACTGCCAATA ATTTTGTAAG CCATCAGAAG AGACCGTATT GTCAAGTACA TAATCCACGG 
AACAACACCT TCACCAGCGT CTATGAAACT CCGGTCAACA TCAATGCTAA GAAGCAGGCA 
GAGGCTGTCA GTGAGATTAA ATACAGAGAA GAGGGTGAGC GTTTCATGTC CACGTTCCAC 
TATGACATGA GATCAAAGGA ACTTGAGCAG GCCCGCAAGG CCAGCCAAAT GACCAACCAG 
GCTTACCAGT CTTTAGGGTT TTCAGAGCAG CAGACATGGT ACTCAGGCAG TGTAACCAGT 
CAGGAGATAG TTCGCATGAG TCAAGCACAG AAGACCATCA GCGATGTGGA GTACAAACGG 
GGCCATGATG AACGAGTTAC ACAGTTCACC TCTGTGACTG ACACACCTGG AATCCTGCAC 
GCTAAGAGTG GCACCTCACT GGCTAGTGAT GTGAAGTACA CAGAGGTTTA TGAGCAGTCC 
AAAGGAAAAG GCAGCTTCCC TGCTATGCTA ACTCCTGGAT ATGAAGTGGC CAAGAAAGCA 
AATACTCTTG CTAGCAATGT GGAGTATAAG AAGGGGCATG AGGAGAGAGT GTCAAAGTAC 
ACCACTGTGA CAGATTCCCC TGAAGTGCTC CTGGCTAAAA GCCAAGGGAG GATTGCAAGC 
GATTTTGTCT ACACTGAAGA TTATGAACAG CAGCGAGGTA AAGGAAGCTT CCCTGCCCAC 
GTCACTCCTG GATACCAAAC AGTCAAGAAA GCAAATGAAA TGGCCAGCAA AGTGAAGTAC 
CAGCAGAAGT ATGAGCAGGA GATGAAGGGT AAAGCGAGTA CAGATGCTGG AGCTGCTGAG 
TTTGCGCTTG CCAAAGAAAA TGCTGAGAAA TTCAGCCAGC ATGCCTATAC TGAGGAATAT 
GAGCAGCAGA GAGGCAAAGG AAGCTTTCCT GCTATGATCA CTCCTGGATA CCAGATGGCC 
AAGAAAGCCA ATGACATTGC CAGCGATCTG AAATATAAGA AGGACCTGAA CAAGATGAAG 
GGCTCCTCTG CCTATCACAG CCTGACAACT GATGACAACC TGACTCTGAA GAACGCCTGC 
AAGATCAATA AGCTTGTCAG CGAGGTTGAA TACAAGAAGG ACCTTGAGAA CACAAAAGGA 
CACAGCATCA ACTACTGCGA AACACCCCAG TTTAAAAATG TGTCCAAGAT CGCCCAGTTC 
ACCAGTGATA ATAAATATAA GGAGAAGTAT ACCAGTCACA TGAAGGGTCA TTATGAGGGG 
ACTGGAGTGG ACAAGAAAAC TGCACATGCC ATGAAAGTCA GAAAGCTGGC CAGTAACATT 
GCATATAAGT CAGACTATGA GCAGGAAAAG ACAGAGCAGG GGGAGTACAA CTACCCAGCT 
GACATCACCC CATCTTACCA GACCCAGAAG AAACTGGAGC CTCTGAAAGA TAAAAATTAC 
AGACAGCACA TTGACAAGCT GAAGTACAGC CAGGTGACAG ACACTCCAGA TATCATTCAA 
GCTCGAATCA ATGCTCAGCA ACTGAGCAAT GTGAACTACA AAGCCGATTA TGAGAAAACT 
AAGACCCAGT ACACCATAGC TCAAGACACG CCTCAGCTCC AGAAGGCCAA AGCCAATGCT 
GAGCTTATCA GTGATATTAA GTATAAAGAG GAATGGGAGA AAAATAAGTC TAAAGTACAT 
GATGTTGGTG CGGACACACT GACCTTAAAA GCTGCCAAAG CCTCTAGAGA TCTGGCCAGC 
GATATCAAAT ATAAGGAATT GTTTGTAAAG AACAAGGACA AAGCAGTGGG TGTGAATGTG 
AGTGACTCCA AAACCCTCCA TTGTCTCCAA GTGGGGAAGC TGAACAGTGA GATTGCCTAT 
AAGAAGAATT CAAAGGAGAC TCAGGCTAAG TATAACCTGA CGAAAGACAT GGTGAATCTA 
AGCCATGCGA AGAAGGCCCA GGCCCTGGCC AGTGATCTGG ACTACAGGAA GAAGCTCCAC 
GAGTACACCA TCCTGCCTGA TGACATCAAA GTTCAGCAGG CTAAGAAGGC CTACAACCTC 
CAGAGTGATA ACCAGTACCG ATCTGACCTG GTCTGGATGA AGGGAGTGGG CTGGGAGGCT 
GAAGGCTGCC TGGATGTTGA ACAGGCCAAG AAGGCTGGAG AGCTTCTGAG CGATAAAAAG 
TATCGGCAGA AGGTGGACAG TGTAAAATTC ACCCAGGTGG CCGACACCCC ATCCATACAA 
CATGCCAAGA AGAGCCAGGA ACTACAGAGT GATCTGACAT ACAAGGCAGG TACAGAGCAG 
ATCATTCATC AGTACACTGT GAGCAAAGAC GAGCCTCTCT ACAAGCAGGC CAAAGCTAAC 
GCGGAGCTTC TCAGCAATAA ATCATATAAG AGTAGCTGGG AAGCCCAGAG GGAGAAGGGC 
TTTGAGCTGA GTATGGATGC ACTGTCTATC CTCACTGCCA AAGCTAAGAG GGATCTTGCC 
AGTGATGTGA AGTATAAGCA GCAGTATGAG ATGACAAAGG GGAAGATGAT AGGGGTGAAG 
AATGTAACAG ATGATTCTCA CTTGGCCCAC TCAGCTCTGG CCACTAAGCT CCAGAGTGAT 
CGCAACTATA AGAAAGAGTA CGAGGATACT AAGACCAAGC ACAGTGCCTC GCTTGATATG 
ATGACCATCA GCCATGCTAA AAAGGCCCAA GACCTGGCCA CAGAGACCAA CTACAGAACC 
ATCCTGCACG AGTACACCAC CCTGCCCAGT GATATGAAGG TGGCATGGGC CAAGAAAGCC 
TACGGCCAAC AAAGTGATAA AAAGTACCGC TCAGATCTGA ACTGGATGAA AGGTGTGGGC 
TGGGAGACCA CTGGATCTCT CGATGTGAAG CAGGCTAGGA AAGCTAGTGA TCTCGCCAGT 
GAGAAAAAGT ACCGCCAGCA TGTTAGTGCT CTGAAGTTCA CCAGCGTGGA GGACACGCCA 
GAGATGGTGC AGGCCAAACT CAGCAATAAG CTGGCACTAG ACAGGCTGTA CAGAGAGAAA 
GGAGAGACTG AGAAGCACAA GTACACAATG ACAGGAGATC TGCCAGAGCA CGTGCAGGCC 
AAGATGAACG CTAGGAACAT CAGTGAGTCA CGATACAAAG AGTCATGGAT GAAGCTCAGA 
GATGGGGGCT ACAAGCTGCG CTTGGATGCT ATTCCATTCC AGGCTGCCAA ATCCTCAGGG 
GAGGTGCTCA GTGATCAAAA GTACAAAGAG GAGTTTCTGA AGATGAAGGG TAAGATGATC 
GGGCTGAAAG CACTCCAGGA TGACATCAAC ATCGCCCACT CTGTGCATGC CAGCCATCTA 
CAGAGTGATA TCAAATATAA GAAGGATTCT ACTAAGGTGC ACTCTCAGTT CCACCTACCG 
ATGGACATGC TGGAAGTAGC CCATGCTAAG AAGGCCCAGG CTCTGGTCAG CGATCAGGAC 
TATAGAATGA CCCTACACAA CTACACTACA CTGCCTGATG ACCTAAATGT GCAGGCTGCT 
AAGAGAGCTT ATTCTCTGCA GAGCGAGAAA GCCTACCGTT CTGATCTGAA TTACCTGCGT 
GGTGCGGCCT GGATTGCCAC CGGCGCCTTA CAAATCGAAG GCTCCAAACA TGCAACACAG 
CTGATCAGCG ATAAAAAGTA TAGACAGCAG CCGTACACAT TTAAGCACAC ATCAGTTACA 
GACTCTCCTG ACATAGTTCA CGCCAAGCTC AGCAACAAGC TCACCAACGA GCGTCTATAC 
AAAGAAAAGG GTGAGAACTT CAGGCATAAC TACACAATCA CAGCTGAGCG ACCAGAAATC 
ACCCAGGCCA AGATCAACGC TGCTAATTAC AGTGATACCA AGTACAAAGA GTCATGGCAC 
ACGCTGAGAG CACAAGGCTA TAAACTGACC ATGCAAGACA TCCCCTTCCA AGCAGCTAAA 
GCATCTACCG ACATTGCCAG TGATTACCAG TACAAGCACA ACCACGTCCT GGAAAAAGGC 
AAGCACATCG GAGCACGGAG CATCATGGAT GACTCTCACC TCCTGCACTG CATGCAGACG 
GCACATCTGC AGAGTGATAA GAATTACCGT AAAGATGCTA TTGCCACCAG CGGCCAGTTT 
CACCTGGGAA TGGACCTGGT TAACCTGGTC CACGCTAAGA AAGCCCAGTC TCTGGCCAGT 
GATCAGGACT ACAAGACCCG CCTGCATTCC TACACTGTCC TGCCTGATGA CATAAAGGTG 
CAGTGGGCCA AGAAGGCCTA TGAGCTGCAG AGTCAGAATG CGTACAAGTC TGATTTGAAC 
TTCATGAAGG GAGTGGCATG GGATTCAGTA GGAGCTCCTC AGCTGGAGTC TGCAAAGAAA 
GCAGGAGAGC TCATCAGCGA TAAGAAGTAT CGTCAGCTTC CTGACAGGCT GAAGTTTACC 
CAGGTTGCAG ACTCACCTGA TATCATTCAT GCTAAGAACA GCTACATGCA GTGTAGTGAG 
AGGCTGTATA AGTCTGGAGA TTCTGACTCC ATGCATAAGT ACACCTTACA TGCTGATCAC 
CCCGACTTTA TCAGAGCAAA GATGAACGCT CAGCAGATCA GTGATAAAGT CTACAAGGCA 
TCAGGAGATC AGGTGAAGAC TGCAGGATAT GACTTGAGGC TAGATGCCAT TCCCTTCCAG 
ACAGCCAAGG CCTCTAGAGA AATTGCAAGT GATTTCCGCT ATAAGGAAGC CTTTGTGAAG 
CAGAAGGGTC AGCAGGTGGG TCTGCTCAGT GTAGAGGACG ACCCCAAGAT GAGGCACTCG 
CTGGCAGTAG GCAAACTGCA GAGCAACAAC GAGTACAAGA AGCAGTTCCA GGAGACCCGC 
TCCCAGTATA AGATCCACGC AGACCAGCCT GAATTCCTGC AAGCAAGGAA GAGCCAGGCT 
CAGGCCAGTG ACCTCAGCTA CCGCCAACGC CTCCACAACT ACACATGTGA CACAGAACAG 
CTCAACTTTA AACACGCCAA AGAGGCCTAC AAGCTGCAGA GTGATGTGAA ATATAAGTCA 
GACCTGAACT GGATAAAGGG AGTTGGCTGG ACTCCTCCGG GCTCTCATAA GGTAGAGATG 
GCTCGGAGGG CAGCAGAACT TGGCCTGGCT GATGGAGTGG ACACTGATGA AGCTATTGCG 
AAGTACCAGC AACTAATGAT GTTCCAGCGC GAGCAGCAGA TGCTAGAGCA GCAGCAGCAG 
CAGCAGGACT TCTCGGATCA GGTGGAAACT GAAGAACATG TCGAACAGTC TGTGGTCAAT 
CCAGATGCCA TGGAGATCCT CCATGTGAAG AGAAAGAAGA CCATCCACAC TACTTTCAAA 
CAAGCAAAGA CTATGACATC GCAGTCTGTA ACATCTCACT CTTCTACAAA GCAGTCCACC 
GAGAGCCAGC ACAGGTCCAT TACAGAGAAA TCCACTGAAA ACAAGTCCAT AACGATGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_007259311.3
CDS	179..5698
Translation	MNIQNCARCGFVVYPAEKINLISQNWHKACFHCEVCKMVLTANNFVSHQKRPYCQVHNPRNNTFTSVYETPVNINAKKQAEAVSEIKYREEGERFMSTFHYDMRSKELEQARKASQMTNQAYQSLGFSEQQTWYSGSVTSQEIVRMSQAQKTISDVEYKRGHDERVTQFTSVTDTPGILHAKSGTSLASDVKYTEVYEQSKGKGSFPAMLTPGYEVAKKANTLASNVEYKKGHEERVSKYTTVTDSPEVLLAKSQGRIASDFVYTEDYEQQRGKGSFPAHVTPGYQTVKKANEMASKVKYQQKYEQEMKGKASTDAGAAEFALAKENAEKFSQHAYTEEYEQQRGKGSFPAMITPGYQMAKKANDIASDLKYKKDLNKMKGSSAYHSLTTDDNLTLKNACKINKLVSEVEYKKDLENTKGHSINYCETPQFKNVSKIAQFTSDNKYKEKYTSHMKGHYEGTGVDKKTAHAMKVRKLASNIAYKSDYEQEKTEQGEYNYPADITPSYQTQKKLEPLKDKNYRQHIDKLKYSQVTDTPDIIQARINAQQLSNVNYKADYEKTKTQYTIAQDTPQLQKAKANAELISDIKYKEEWEKNKSKVHDVGADTLTLKAAKASRDLASDIKYKELFVKNKDKAVGVNVSDSKTLHCLQVGKLNSEIAYKKNSKETQAKYNLTKDMVNLSHAKKAQALASDLDYRKKLHEYTILPDDIKVQQAKKAYNLQSDNQYRSDLVWMKGVGWEAEGCLDVEQAKKAGELLSDKKYRQKVDSVKFTQVADTPSIQHAKKSQELQSDLTYKAGTEQIIHQYTVSKDEPLYKQAKANAELLSNKSYKSSWEAQREKGFELSMDALSILTAKAKRDLASDVKYKQQYEMTKGKMIGVKNVTDDSHLAHSALATKLQSDRNYKKEYEDTKTKHSASLDMMTISHAKKAQDLATETNYRTILHEYTTLPSDMKVAWAKKAYGQQSDKKYRSDLNWMKGVGWETTGSLDVKQARKASDLASEKKYRQHVSALKFTSVEDTPEMVQAKLSNKLALDRLYREKGETEKHKYTMTGDLPEHVQAKMNARNISESRYKESWMKLRDGGYKLRLDAIPFQAAKSSGEVLSDQKYKEEFLKMKGKMIGLKALQDDINIAHSVHASHLQSDIKYKKDSTKVHSQFHLPMDMLEVAHAKKAQALVSDQDYRMTLHNYTTLPDDLNVQAAKRAYSLQSEKAYRSDLNYLRGAAWIATGALQIEGSKHATQLISDKKYRQQPYTFKHTSVTDSPDIVHAKLSNKLTNERLYKEKGENFRHNYTITAERPEITQAKINAANYSDTKYKESWHTLRAQGYKLTMQDIPFQAAKASTDIASDYQYKHNHVLEKGKHIGARSIMDDSHLLHCMQTAHLQSDKNYRKDAIATSGQFHLGMDLVNLVHAKKAQSLASDQDYKTRLHSYTVLPDDIKVQWAKKAYELQSQNAYKSDLNFMKGVAWDSVGAPQLESAKKAGELISDKKYRQLPDRLKFTQVADSPDIIHAKNSYMQCSERLYKSGDSDSMHKYTLHADHPDFIRAKMNAQQISDKVYKASGDQVKTAGYDLRLDAIPFQTAKASREIASDFRYKEAFVKQKGQQVGLLSVEDDPKMRHSLAVGKLQSNNEYKKQFQETRSQYKIHADQPEFLQARKSQAQASDLSYRQRLHNYTCDTEQLNFKHAKEAYKLQSDVKYKSDLNWIKGVGWTPPGSHKVEMARRAAELGLADGVDTDEAIAKYQQLMMFQREQQMLEQQQQQQDFSDQVETEEHVEQSVVNPDAMEILHVKRKKTIHTTFKQAKTMTSQSVTSHSSTKQSTESQHRSITEKSTENKSITM

Target ORF information:

RefSeq Version	XM_007259249.2
Organism	Astyanax mexicanus(Mexican tetra)
Definition	Astyanax mexicanus nebulin related anchoring protein (nrap), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_007259249.2

ORF Insert Sequence:

ATGAATATTC AAAACTGTGC CAGGTGTGGG TTTGTGGTGT ACCCAGCTGA GAAGATCAAT 
CTCATTAGTC AGAACTGGCA CAAAGCATGC TTCCACTGTG AGGTCTGTAA GATGGTTCTA 
ACTGCCAATA ATTTTGTAAG CCATCAGAAG AGACCGTATT GTCAAGTACA TAATCCACGG 
AACAACACCT TCACCAGCGT CTATGAAACT CCGGTCAACA TCAATGCTAA GAAGCAGGCA 
GAGGCTGTCA GTGAGATTAA ATACAGAGAA GAGGGTGAGC GTTTCATGTC CACGTTCCAC 
TATGACATGA GATCAAAGGA ACTTGAGCAG GCCCGCAAGG CCAGCCAAAT GACCAACCAG 
GCTTACCAGT CTTTAGGGTT TTCAGAGCAG CAGACATGGT ACTCAGGCAG TGTAACCAGT 
CAGGAGATAG TTCGCATGAG TCAAGCACAG AAGACCATCA GCGATGTGGA GTACAAACGG 
GGCCATGATG AACGAGTTAC ACAGTTCACC TCTGTGACTG ACACACCTGG AATCCTGCAC 
GCTAAGAGTG GCACCTCACT GGCTAGTGAT GTGAAGTACA CAGAGGTTTA TGAGCAGTCC 
AAAGGAAAAG GCAGCTTCCC TGCTATGCTA ACTCCTGGAT ATGAAGTGGC CAAGAAAGCA 
AATACTCTTG CTAGCAATGT GGAGTATAAG AAGGGGCATG AGGAGAGAGT GTCAAAGTAC 
ACCACTGTGA CAGATTCCCC TGAAGTGCTC CTGGCTAAAA GCCAAGGGAG GATTGCAAGC 
GATTTTGTCT ACACTGAAGA TTATGAACAG CAGCGAGGTA AAGGAAGCTT CCCTGCCCAC 
GTCACTCCTG GATACCAAAC AGTCAAGAAA GCAAATGAAA TGGCCAGCAA AGTGAAGTAC 
CAGCAGAAGT ATGAGCAGGA GATGAAGGGT AAAGCGAGTA CAGATGCTGG AGCTGCTGAG 
TTTGCGCTTG CCAAAGAAAA TGCTGAGAAA TTCAGCCAGC ATGCCTATAC TGAGGAATAT 
GAGCAGCAGA GAGGCAAAGG AAGCTTTCCT GCTATGATCA CTCCTGGATA CCAGATGGCC 
AAGAAAGCCA ATGACATTGC CAGCGATCTG AAATATAAGA AGGACCTGAA CAAGATGAAG 
GGCTCCTCTG CCTATCACAG CCTGACAACT GATGACAACC TGACTCTGAA GAACGCCTGC 
AAGATCAATA AGCTTGTCAG CGAGGTTGAA TACAAGAAGG ACCTTGAGAA CACAAAAGGA 
CACAGCATCA ACTACTGCGA AACACCCCAG TTTAAAAATG TGTCCAAGAT CGCCCAGTTC 
ACCAGTGATA ATAAATATAA GGAGAAGTAT ACCAGTCACA TGAAGGGTCA TTATGAGGGG 
ACTGGAGTGG ACAAGAAAAC TGCACATGCC ATGAAAGTCA GAAAGCTGGC CAGTAACATT 
GCATATAAGT CAGACTATGA GCAGGAAAAG ACAGAGCAGG GGGAGTACAA CTACCCAGCT 
GACATCACCC CATCTTACCA GACCCAGAAG AAACTGGAGC CTCTGAAAGA TAAAAATTAC 
AGACAGCACA TTGACAAGCT GAAGTACAGC CAGGTGACAG ACACTCCAGA TATCATTCAA 
GCTCGAATCA ATGCTCAGCA ACTGAGCAAT GTGAACTACA AAGCCGATTA TGAGAAAACT 
AAGACCCAGT ACACCATAGC TCAAGACACG CCTCAGCTCC AGAAGGCCAA AGCCAATGCT 
GAGCTTATCA GTGATATTAA GTATAAAGAG GAATGGGAGA AAAATAAGTC TAAAGTACAT 
GATGTTGGTG CGGACACACT GACCTTAAAA GCTGCCAAAG CCTCTAGAGA TCTGGCCAGC 
GATATCAAAT ATAAGGAATT GTTTGTAAAG AACAAGGACA AAGCAGTGGG TGTGAATGTG 
AGTGACTCCA AAACCCTCCA TTGTCTCCAA GTGGGGAAGC TGAACAGTGA GATTGCCTAT 
AAGAAGAATT CAAAGGAGAC TCAGGCTAAG TATAACCTGA CGAAAGACAT GGTGAATCTA 
AGCCATGCGA AGAAGGCCCA GGCCCTGGCC AGTGATCTGG ACTACAGGAA GAAGCTCCAC 
GAGTACACCA TCCTGCCTGA TGACATCAAA GTTCAGCAGG CTAAGAAGGC CTACAACCTC 
CAGAGTGATA ACCAGTACCG ATCTGACCTG GTCTGGATGA AGGGAGTGGG CTGGGAGGCT 
GAAGGCTGCC TGGATGTTGA ACAGGCCAAG AAGGCTGGAG AGCTTCTGAG CGATAAAAAG 
TATCGGCAGA AGGTGGACAG TGTAAAATTC ACCCAGGTGG CCGACACCCC ATCCATACAA 
CATGCCAAGA AGAGCCAGGA ACTACAGAGT GATCTGACAT ACAAGGCAGG TACAGAGCAG 
ATCATTCATC AGTACACTGT GAGCAAAGAC GAGCCTCTCT ACAAGCAGGC CAAAGCTAAC 
GCGGAGCTTC TCAGCAATAA ATCATATAAG AGTAGCTGGG AAGCCCAGAG GGAGAAGGGC 
TTTGAGCTGA GTATGGATGC ACTGTCTATC CTCACTGCCA AAGCTAAGAG GGATCTTGCC 
AGTGATGTGA AGTATAAGCA GCAGTATGAG ATGACAAAGG GGAAGATGAT AGGGGTGAAG 
AATGTAACAG ATGATTCTCA CTTGGCCCAC TCAGCTCTGG CCACTAAGCT CCAGAGTGAT 
CGCAACTATA AGAAAGAGTA CGAGGATACT AAGACCAAGC ACAGTGCCTC GCTTGATATG 
ATGACCATCA GCCATGCTAA AAAGGCCCAA GACCTGGCCA CAGAGACCAA CTACAGAACC 
ATCCTGCACG AGTACACCAC CCTGCCCAGT GATATGAAGG TGGCATGGGC CAAGAAAGCC 
TACGGCCAAC AAAGTGATAA AAAGTACCGC TCAGATCTGA ACTGGATGAA AGGTGTGGGC 
TGGGAGACCA CTGGATCTCT CGATGTGAAG CAGGCTAGGA AAGCTAGTGA TCTCGCCAGT 
GAGAAAAAGT ACCGCCAGCA TGTTAGTGCT CTGAAGTTCA CCAGCGTGGA GGACACGCCA 
GAGATGGTGC AGGCCAAACT CAGCAATAAG CTGGCACTAG ACAGGCTGTA CAGAGAGAAA 
GGAGAGACTG AGAAGCACAA GTACACAATG ACAGGAGATC TGCCAGAGCA CGTGCAGGCC 
AAGATGAACG CTAGGAACAT CAGTGAGTCA CGATACAAAG AGTCATGGAT GAAGCTCAGA 
GATGGGGGCT ACAAGCTGCG CTTGGATGCT ATTCCATTCC AGGCTGCCAA ATCCTCAGGG 
GAGGTGCTCA GTGATCAAAA GTACAAAGAG GAGTTTCTGA AGATGAAGGG TAAGATGATC 
GGGCTGAAAG CACTCCAGGA TGACATCAAC ATCGCCCACT CTGTGCATGC CAGCCATCTA 
CAGAGTGATA TCAAATATAA GAAGGATTCT ACTAAGGTGC ACTCTCAGTT CCACCTACCG 
ATGGACATGC TGGAAGTAGC CCATGCTAAG AAGGCCCAGG CTCTGGTCAG CGATCAGGAC 
TATAGAATGA CCCTACACAA CTACACTACA CTGCCTGATG ACCTAAATGT GCAGGCTGCT 
AAGAGAGCTT ATTCTCTGCA GAGCGAGAAA GCCTACCGTT CTGATCTGAA TTACCTGCGT 
GGTGCGGCCT GGATTGCCAC CGGCGCCTTA CAAATCGAAG GCTCCAAACA TGCAACACAG 
CTGATCAGCG ATAAAAAGTA TAGACAGCAG CCGTACACAT TTAAGCACAC ATCAGTTACA 
GACTCTCCTG ACATAGTTCA CGCCAAGCTC AGCAACAAGC TCACCAACGA GCGTCTATAC 
AAAGAAAAGG GTGAGAACTT CAGGCATAAC TACACAATCA CAGCTGAGCG ACCAGAAATC 
ACCCAGGCCA AGATCAACGC TGCTAATTAC AGTGATACCA AGTACAAAGA GTCATGGCAC 
ACGCTGAGAG CACAAGGCTA TAAACTGACC ATGCAAGACA TCCCCTTCCA AGCAGCTAAA 
GCATCTACCG ACATTGCCAG TGATTACCAG TACAAGCACA ACCACGTCCT GGAAAAAGGC 
AAGCACATCG GAGCACGGAG CATCATGGAT GACTCTCACC TCCTGCACTG CATGCAGACG 
GCACATCTGC AGAGTGATAA GAATTACCGT AAAGATGCTA TTGCCACCAG CGGCCAGTTT 
CACCTGGGAA TGGACCTGGT TAACCTGGTC CACGCTAAGA AAGCCCAGTC TCTGGCCAGT 
GATCAGGACT ACAAGACCCG CCTGCATTCC TACACTGTCC TGCCTGATGA CATAAAGGTG 
CAGTGGGCCA AGAAGGCCTA TGAGCTGCAG AGTCAGAATG CGTACAAGTC TGATTTGAAC 
TTCATGAAGG GAGTGGCATG GGATTCAGTA GGAGCTCCTC AGCTGGAGTC TGCAAAGAAA 
GCAGGAGAGC TCATCAGCGA TAAGAAGTAT CGTCAGCTTC CTGACAGGCT GAAGTTTACC 
CAGGTTGCAG ACTCACCTGA TATCATTCAT GCTAAGAACA GCTACATGCA GTGTAGTGAG 
AGGCTGTATA AGTCTGGAGA TTCTGACTCC ATGCATAAGT ACACCTTACA TGCTGATCAC 
CCCGACTTTA TCAGAGCAAA GATGAACGCT CAGCAGATCA GTGATAAAGT CTACAAGGCA 
TCAGGAGATC AGGTGAAGAC TGCAGGATAT GACTTGAGGC TAGATGCCAT TCCCTTCCAG 
ACAGCCAAGG CCTCTAGAGA AATTGCAAGT GATTTCCGCT ATAAGGAAGC CTTTGTGAAG 
CAGAAGGGTC AGCAGGTGGG TCTGCTCAGT GTAGAGGACG ACCCCAAGAT GAGGCACTCG 
CTGGCAGTAG GCAAACTGCA GAGCAACAAC GAGTACAAGA AGCAGTTCCA GGAGACCCGC 
TCCCAGTATA AGATCCACGC AGACCAGCCT GAATTCCTGC AAGCAAGGAA GAGCCAGGCT 
CAGGCCAGTG ACCTCAGCTA CCGCCAACGC CTCCACAACT ACACATGTGA CACAGAACAG 
CTCAACTTTA AACACGCCAA AGAGGCCTAC AAGCTGCAGA GTGATGTGAA ATATAAGTCA 
GACCTGAACT GGATAAAGGG AGTTGGCTGG ACTCCTCCGG GCTCTCATAA GGTAGAGATG 
GCTCGGAGGG CAGCAGAACT TGGCCTGGCT GATGGAGTGG ACACTGATGA AGCTATTGCG 
AAGTACCAGC AACTAATGAT GTTCCAGCGC GAGCAGCAGA TGCTAGAGCA GCAGCAGCAG 
CAGCAGGACT TCTCGGATCA GGTGGAAACT GAAGAACATG TCGAACAGTC TGTGGTCAAT 
CCAGATGCCA TGGAGATCCT CCATGTGAAG AGAAAGAAGA CCATCCACAC TACTTTCAAA 
CAAGCAAAGA CTATGACATC GCAGTCTGTA ACATCTCACT CTTCTACAAA GCAGTCCACC 
GAGAGCCAGC ACAGGTCCAT TACAGAGAAA TCCACTGAAA ACAAGTCCAT AACGATGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

nrap ( XM_007259249.1 ) cDNA ORF clone, Astyanax mexicanus(Mexican tetra) -> XP_007259311.2 Astyanax mexicanus nebulin related anchoring protein (nrap), mRNA.

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CloneID	OAs40986
Clone ID Related Accession (Same CDS sequence)	XM_007259249.1
Accession Version	XM_007259249.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5520bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1455033600000
Organism	Astyanax mexicanus(Mexican tetra)
Product	nebulin-related-anchoring protein
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_006749457.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Astyanax mexicanus Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAATATTC AAAACTGTGC CAGGTGTGGG TTTGTGGTGT ACCCAGCTGA GAAGATCAGT 
CTCATTAGTC AGAACTGGCA CAAAGCATGC TTCCACTGTG AGGTCTGTAA GATGGTTCTA 
ACTGCCAATA ATTTTGTAAG CCATCAGAAG AGACCGTATT GTCAAGTACA TAATCCACGG 
AACAACACCT TCACCAGCGT CTATGAAACT CCGGTCAACA TCAATGCTAA GAAGCAGGCA 
GAGGCTGTCA GTGAGATTAA ATACAGAGAA GAGGGTGAGC GTTTCATGTC CACGTTCCAC 
TATGACATGA GATCAAAGGA ACTTGAGCAG GCCCGCAAGG CCAGCCAAAT GACCAACCAG 
GCTTACCAGT CTTCAGGGTT TTCAGAGCAG CAGACATGGT ACTCAGGCAG TGTAACCAGT 
CAGGAGATAG TTCGCGTGAG TCAAGCACAG AAGACCATCA GCGATGTGGA GTACAAACGG 
GGCCATGATG AACGAGTTAC ACAGTTCACC TCTGTGACTG ACACACCTGG AATCCTGCAC 
GCTAAGAGTG GCACCTCACT GGCTAGTGAT GTGAAGTACA CAGAGGTTTA TGAGCAGTCC 
AAAGGAAAAG GCAGCTTCCC TGCTATGCTA ACTCCTGGAT ATGAAGTGGC CAAGAAAGCA 
AATACTCTTG CTAGCAATGT GGAGTATAAG AAGGGGCATG AGGAGAGAGT GTCAAAGTAC 
ACCACTGTGA CAGATTCCCC TGAAGTGCTC CTGGCTAAAA GCCAAGGGAG GATTGCAAGC 
GATTTTGTCT ACACTGAAGA TTATGAACAG CAGCGAGGTA AAGGAAGCTT CCCTGCCCAC 
GTCACTCCTG GATACCAAAC AGTCAAGAAA GCAAATGAAA TGGCCAGCAA AGTGAAGTAC 
CAGCAGAAGT ATGAGCAGGA GATGAAGGGT AAAGCGAGTA CAGATGCTGG AGCTGCTGAG 
TTTGCGCTTG CCAAAGAAAA TGCTGAGAAA TTCAGCCAGC ATGCCTATAC TGAGGAATAT 
GAGCAGCAGA GAGGCAAAGG AAGCTTTCCT GCTATGATCA CTCCTGGATA CCAGATGGCC 
AAGAAAGCCA ATGACATTGC CAGCGATCTG AAATATAAGA AGGACCTGAA CAAGATGAAG 
GGCTCCTCTG CCTATCACAG CCTGACAACT GATGACAACC TGACTCTGAA GAACGCCCGC 
AAGATCAATA AGCTTGTAAG CGAGGTTGAA TACAAGAAGG ACCTTGAGAA CACAAAAGGA 
CACAGCATCA ACTACTGCGA AACACCCCAG TTTAAAACTG TGTCCAAGAT CGCCCAGTTC 
ACCAGTGATA ATAAATATAA GGAGAAGTAT ACCAGTCACA TGAAGGGTCA TTATGAGGGG 
ACTGGAGTGG ACAAGAAAAC TGCACATGCC ATGAAAGTCA GAAAGCTGGC CAGTAACATT 
GCATATAAGT CAGACTATGA GCAGGAAAAG ACAGAGCAGG GGGAGTACAA CTACCCAGCT 
GACATCACCC CATCTTACCA GACCCAGAAG AAACTGGAGC CTCTGAAAGA TAAAAATTAC 
AGACAGCACA TTGACAAGCT GAAGTACAGC CAGGTGACAG ACACTCCAGA TATCATTCAA 
GCTCGAATCA ATGCTCAGCA ACTGAGCAAT GTGAACTACA AAGCCGATTA TGAGAAAACT 
AAGACCCAGT ACACCATAGC TCAAGACACG CCTCAGCTCC AGAAGGCCAA AGCCAATGCT 
GAGCTTATCA GTGATATTAA GTATAAAGAG GAATGGGAGA AAAATAAGTC TAAAGTACAT 
GATGTTGGTG CGGACACACT GACCTTAAAA GCTGCCAAAG CCTCTAGAGA TCTGGCCAGC 
GATATCAAAT ATAAGGAATC GTTTGTAAAG AACAAGGACA AAGCAGTGGG TGTGAATGTG 
AGTGACTCCA AAACCCTCCA TTGTCTCCAA GTGGGGAAGC TGAACAGTGA GATTGCCTAT 
AAGAAGAATT CAAAGGAGAC TCAGGCTAAG TATAACCTGA CGAAAGACAT GGTGAATCTA 
AGCCATGCGA AGAAGGCCCA GGCCCTGGCC AGTGATCTGG ACTACAGGAA GAAGCTCCAC 
GAGTACACCA TCCTGCCTGA TGACATCAAA GTTCAGCAGG CTAAGAAGGC CTACAACCTC 
CAGAGTGATA ACCAGTACCG ATCTGACCTG GTCTGGATGA AGGGAGTGGG CTGGGAGGCT 
GAAGGCTGCC TGGATGTTGA ACAGGCCAAG AAGGCTGGAG AGCTTCTGAG CGATAAAAAG 
TATCGGCAGA AGGTGGACAG TGTAAAATTC ACCCAGGTGG CCGACACCCC ATCCATACAA 
CATGCCAAGA AGAGCCAGGA ACTACAGAGT GATCTGACAT ACAAGGCAGG TACAGAGCAG 
ATCATTCATC AGTACACTGT GAGCAAAGAC GAGCCTCTCT ACAAGCAGGC CAAAGCTAAC 
GCGGAGCTTC TCAGCAATAA ATCATATAAG AGTAGCTGGG AAGCCCAGAG GGAGAAGGGC 
TTTGAGCTGA GTATGGATGC ACTGTCTATC CTCACTGCCA AAGCTAAGAG GGATCTTGCC 
AGTGATGTGA AGTATAAGCA GCAGTATGAG ATGACAAAGG GGAAGATGAT AGGGGTGAAG 
AATGTAACAG ATGATTCTCA TTTGGCCCAC TCAGCTCTGG CCACTAAGCT CCAGAGTGAT 
CGCAACTATA AGAAAGAGTA CGAGGATACT AAGACCAAGC ACAGTGCCTC GCTTGATATG 
ATGACCATCA GCCATGCTAA AAAGGCCCAA GACCTGGCCA CAGAGACCAA CTACAGAACC 
ATCCTGCACG AGTACACCAC CCTGCCCAGT GATATGAAGG TGGCATGGGC CAAGAAAGCC 
TACGGCCAAC AAAGTGATAA AAAGTACCGC TCAGATCTGA ACTGGATGAA AGGTGTGGGC 
TGGGAGACCA CTGGATCTCT CGATGTGAAG CAGGCTAGGA AAGCTAGTGA TCTCGCCAGT 
GAGAAAAAGT ACCGCCAGCA TGTTAGTGCT CTGAAGTTCA CCAGCGTGGA GGACACGCCA 
GAGATGGTGC AGGCCAAACT CAGCAATAAG CTGGCACTAG ACAGGCTGTA CAGAGAGAAA 
GGAGAGACTG AGAAGCACAA GTACACAATG ACAGGAGATC TGCCAGAGCA CGTGCAGGCC 
AAGATGAACG CTATGAACAT CAGTGAGTCA CGATACAAAG AGTCATGGAT GAAGCTCAGA 
GATGGGGGCT ACAAGCTGCG CTTGGATGCT ATTCCATTCC AGGCTGCCAA ATCCTCAGGG 
GAGGTGCTCA GTGATCAAAA GTACAAAGAG GAGTTTCTGA AGATGAAGGG TAAGATGATC 
GGGCTGAAAG CACTCCAGGA TGACATCAAC ATCGCCCACT CTGTGCATGC CAGCCATCTA 
CAGAGTGATA TCAAATATAA GAAGGATTCT ACTAAGGTGC ACTCTCAGTT CCACCTACCG 
ATGGACATGC TGGAAGTAGC CCATGCTAAG AAGGCCCAGG CTCTGGTCAG CGATCAGGAC 
TATAGAATGA CCCTACACAA CTACACTACA CTGCCTGATG ACCTAAATGT GCAGGCTGCT 
AAGAGAGCTT ATTCTCTGCA GAGCGAGAAA GCCTACCGTT CTGATCTGAA TTACCTGCGT 
GGTGCGGCCT GGATTGCCAC CGGCGCCTTA CAAATCGAAG GCTCCAAACA TGCAACACAG 
CTGATCAGCG ATAAAAAGTA TAGACAGCAG CCGTACACAT TTAAGCACAC ATCAGTTACA 
GACTCTCCTG ACATAGTTCA CGCCAAGCTC AGCAACAAGC TCACCAACGA GCGTCTATAC 
AAAGAAAAGG GTGAGAACTT CAGGCATAAC TACACAATCA CAGCTGAGCG ACCAGAAATC 
ACCCAGGCCA AGATCAACGC TGCTAATTAC AGTGATACCA AGTACAAAGA GTCATGGCAC 
ACGCTGAGAG CACAAGGCTA TAAACTGACC ATGCAAGACA TCCCCTTCCA AGCAGCCAAA 
GCATCTACCG ACATTGCCAG TGATTACCAG TACAAGCACA ACCACGTCCT GGAAAAAGGC 
AAGCACATCG GAGCACGGAG CATCATGGAT GACTCTCACC TCCTGCACTG CATGCAGACG 
GCACATCTGC AGAGTGATAA GAATTACCGT AAAGATGCTA TTGCCACCAG CGGCCAGTTT 
CATCTGGGAA TGGACCTGGT TAACCTGGTC CACGCTAAGA AAGCCCAGTC TCTGGCCAGT 
GACCAGGACT ACAAGACCCG CCTGCATTCC TACACTGTCC TGCCTGATGA CATAAAGGTG 
CAGTGGGCCA AGAAGGCCTA TGAGCTGCAG AGTCAGAATG CGTACAAGTC TGATTTGAAC 
TTCATGAAGG GAGTGGCATG GGATTCAGTA GGAGCTCCTC AGCTGGAGTC TGCAAAGAAA 
GCAGGAGAGC TCATCAGCGA TAAGAAGTAT CGTCAGCTTC CTGACAGGCT GAAGTTTACC 
CAGGTTGCAG ACTCACCTGA TATCATTCAT GCTAAGAACA GCTACATGCA GTGTAGTGAG 
AGGCTGTATA AGTCTGGAGA TTCTGACTCC ATGCATAAGT ACACCTTACA TGCTGATCAC 
CCCGACTTTA TCAGAGCAAA GATGAACGCT CAGCAGATCA GTGATAAAGT CTACAAGGCA 
TCAGGAGATC AGGTGAAGAC TGCAGGATAT GACTTGAGGC TAGATGCCAT TCCCTTCCAG 
ACAGCCAAGG CCTCTAGAGA AATTGCAAGT GATTTCCGCT ATAAGGAAGC CTTTGTGAAG 
CAGAAGGGTC AGCAGGTGGG TCTGCTCAGT GTAGAGGACG ACCCCAAGAT GAGGCACTCG 
CTGGCTGTAG GCAAACTGCA GAGCGACAAT GAGTACAAGA AGCAGTTCCA GGAGACCCGC 
TCCCAGTATA AGATCCACGC AGACCAGCCT GAATTCCTGC AAGCAAGGAA GAGCCAGGCT 
CAGGCCAGTG ACCTCAGCTA CCGCCAACGC CTCCACAACT ACACATGTGA CCCAGAACAG 
CTCAACTTTA AACACGCCAA AGAGGCCTAC AAGCTGCAGA GTGATGTGAA ATATAAGTCA 
GACCTGAACT GGATAAAGGG AGTTGGCTGG ACTCCTCCGG GCTCTCATAA GGTAGAGATG 
GCTCGGAGGG CAGCAGAACT TGGCCTGGCT GATGGAGTGG ACACTGATGA AGCTATTGCG 
AAGTACCAGC AACTAATGAT GTTCCAGCGC GAGCAGCAGA TGCTAGAGCA GCAGCAGCAG 
CAGCAGGACT TCTCGGATCA GGTGGAAACT GAAGAACATG TCGAACAGTC TGTGGTCAAT 
CCAGATGCCA TGGAGATCCT CCATGTGAAG AGAAAGAAGA CCATCCACAC TACTTTCAAA 
CAAGCAAAGA CTATGGCATC GCAGTCTGTA ACATCTCACT CTTCTACAAA GCAGTCCACT 
GAGAGCCAGC ACAGGTCCAT TACAGAGAAA TCCACTGAAA ACAAGTCCAT AACGATGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_007259311.2
CDS	179..5698
Translation	MNIQNCARCGFVVYPAEKISLISQNWHKACFHCEVCKMVLTANNFVSHQKRPYCQVHNPRNNTFTSVYETPVNINAKKQAEAVSEIKYREEGERFMSTFHYDMRSKELEQARKASQMTNQAYQSSGFSEQQTWYSGSVTSQEIVRVSQAQKTISDVEYKRGHDERVTQFTSVTDTPGILHAKSGTSLASDVKYTEVYEQSKGKGSFPAMLTPGYEVAKKANTLASNVEYKKGHEERVSKYTTVTDSPEVLLAKSQGRIASDFVYTEDYEQQRGKGSFPAHVTPGYQTVKKANEMASKVKYQQKYEQEMKGKASTDAGAAEFALAKENAEKFSQHAYTEEYEQQRGKGSFPAMITPGYQMAKKANDIASDLKYKKDLNKMKGSSAYHSLTTDDNLTLKNARKINKLVSEVEYKKDLENTKGHSINYCETPQFKTVSKIAQFTSDNKYKEKYTSHMKGHYEGTGVDKKTAHAMKVRKLASNIAYKSDYEQEKTEQGEYNYPADITPSYQTQKKLEPLKDKNYRQHIDKLKYSQVTDTPDIIQARINAQQLSNVNYKADYEKTKTQYTIAQDTPQLQKAKANAELISDIKYKEEWEKNKSKVHDVGADTLTLKAAKASRDLASDIKYKESFVKNKDKAVGVNVSDSKTLHCLQVGKLNSEIAYKKNSKETQAKYNLTKDMVNLSHAKKAQALASDLDYRKKLHEYTILPDDIKVQQAKKAYNLQSDNQYRSDLVWMKGVGWEAEGCLDVEQAKKAGELLSDKKYRQKVDSVKFTQVADTPSIQHAKKSQELQSDLTYKAGTEQIIHQYTVSKDEPLYKQAKANAELLSNKSYKSSWEAQREKGFELSMDALSILTAKAKRDLASDVKYKQQYEMTKGKMIGVKNVTDDSHLAHSALATKLQSDRNYKKEYEDTKTKHSASLDMMTISHAKKAQDLATETNYRTILHEYTTLPSDMKVAWAKKAYGQQSDKKYRSDLNWMKGVGWETTGSLDVKQARKASDLASEKKYRQHVSALKFTSVEDTPEMVQAKLSNKLALDRLYREKGETEKHKYTMTGDLPEHVQAKMNAMNISESRYKESWMKLRDGGYKLRLDAIPFQAAKSSGEVLSDQKYKEEFLKMKGKMIGLKALQDDINIAHSVHASHLQSDIKYKKDSTKVHSQFHLPMDMLEVAHAKKAQALVSDQDYRMTLHNYTTLPDDLNVQAAKRAYSLQSEKAYRSDLNYLRGAAWIATGALQIEGSKHATQLISDKKYRQQPYTFKHTSVTDSPDIVHAKLSNKLTNERLYKEKGENFRHNYTITAERPEITQAKINAANYSDTKYKESWHTLRAQGYKLTMQDIPFQAAKASTDIASDYQYKHNHVLEKGKHIGARSIMDDSHLLHCMQTAHLQSDKNYRKDAIATSGQFHLGMDLVNLVHAKKAQSLASDQDYKTRLHSYTVLPDDIKVQWAKKAYELQSQNAYKSDLNFMKGVAWDSVGAPQLESAKKAGELISDKKYRQLPDRLKFTQVADSPDIIHAKNSYMQCSERLYKSGDSDSMHKYTLHADHPDFIRAKMNAQQISDKVYKASGDQVKTAGYDLRLDAIPFQTAKASREIASDFRYKEAFVKQKGQQVGLLSVEDDPKMRHSLAVGKLQSDNEYKKQFQETRSQYKIHADQPEFLQARKSQAQASDLSYRQRLHNYTCDPEQLNFKHAKEAYKLQSDVKYKSDLNWIKGVGWTPPGSHKVEMARRAAELGLADGVDTDEAIAKYQQLMMFQREQQMLEQQQQQQDFSDQVETEEHVEQSVVNPDAMEILHVKRKKTIHTTFKQAKTMASQSVTSHSSTKQSTESQHRSITEKSTENKSITM

Target ORF information:

RefSeq Version	XM_007259249.1
Organism	Astyanax mexicanus(Mexican tetra)
Definition	Astyanax mexicanus nebulin related anchoring protein (nrap), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_007259249.1

ORF Insert Sequence:

ATGAATATTC AAAACTGTGC CAGGTGTGGG TTTGTGGTGT ACCCAGCTGA GAAGATCAGT 
CTCATTAGTC AGAACTGGCA CAAAGCATGC TTCCACTGTG AGGTCTGTAA GATGGTTCTA 
ACTGCCAATA ATTTTGTAAG CCATCAGAAG AGACCGTATT GTCAAGTACA TAATCCACGG 
AACAACACCT TCACCAGCGT CTATGAAACT CCGGTCAACA TCAATGCTAA GAAGCAGGCA 
GAGGCTGTCA GTGAGATTAA ATACAGAGAA GAGGGTGAGC GTTTCATGTC CACGTTCCAC 
TATGACATGA GATCAAAGGA ACTTGAGCAG GCCCGCAAGG CCAGCCAAAT GACCAACCAG 
GCTTACCAGT CTTCAGGGTT TTCAGAGCAG CAGACATGGT ACTCAGGCAG TGTAACCAGT 
CAGGAGATAG TTCGCGTGAG TCAAGCACAG AAGACCATCA GCGATGTGGA GTACAAACGG 
GGCCATGATG AACGAGTTAC ACAGTTCACC TCTGTGACTG ACACACCTGG AATCCTGCAC 
GCTAAGAGTG GCACCTCACT GGCTAGTGAT GTGAAGTACA CAGAGGTTTA TGAGCAGTCC 
AAAGGAAAAG GCAGCTTCCC TGCTATGCTA ACTCCTGGAT ATGAAGTGGC CAAGAAAGCA 
AATACTCTTG CTAGCAATGT GGAGTATAAG AAGGGGCATG AGGAGAGAGT GTCAAAGTAC 
ACCACTGTGA CAGATTCCCC TGAAGTGCTC CTGGCTAAAA GCCAAGGGAG GATTGCAAGC 
GATTTTGTCT ACACTGAAGA TTATGAACAG CAGCGAGGTA AAGGAAGCTT CCCTGCCCAC 
GTCACTCCTG GATACCAAAC AGTCAAGAAA GCAAATGAAA TGGCCAGCAA AGTGAAGTAC 
CAGCAGAAGT ATGAGCAGGA GATGAAGGGT AAAGCGAGTA CAGATGCTGG AGCTGCTGAG 
TTTGCGCTTG CCAAAGAAAA TGCTGAGAAA TTCAGCCAGC ATGCCTATAC TGAGGAATAT 
GAGCAGCAGA GAGGCAAAGG AAGCTTTCCT GCTATGATCA CTCCTGGATA CCAGATGGCC 
AAGAAAGCCA ATGACATTGC CAGCGATCTG AAATATAAGA AGGACCTGAA CAAGATGAAG 
GGCTCCTCTG CCTATCACAG CCTGACAACT GATGACAACC TGACTCTGAA GAACGCCCGC 
AAGATCAATA AGCTTGTAAG CGAGGTTGAA TACAAGAAGG ACCTTGAGAA CACAAAAGGA 
CACAGCATCA ACTACTGCGA AACACCCCAG TTTAAAACTG TGTCCAAGAT CGCCCAGTTC 
ACCAGTGATA ATAAATATAA GGAGAAGTAT ACCAGTCACA TGAAGGGTCA TTATGAGGGG 
ACTGGAGTGG ACAAGAAAAC TGCACATGCC ATGAAAGTCA GAAAGCTGGC CAGTAACATT 
GCATATAAGT CAGACTATGA GCAGGAAAAG ACAGAGCAGG GGGAGTACAA CTACCCAGCT 
GACATCACCC CATCTTACCA GACCCAGAAG AAACTGGAGC CTCTGAAAGA TAAAAATTAC 
AGACAGCACA TTGACAAGCT GAAGTACAGC CAGGTGACAG ACACTCCAGA TATCATTCAA 
GCTCGAATCA ATGCTCAGCA ACTGAGCAAT GTGAACTACA AAGCCGATTA TGAGAAAACT 
AAGACCCAGT ACACCATAGC TCAAGACACG CCTCAGCTCC AGAAGGCCAA AGCCAATGCT 
GAGCTTATCA GTGATATTAA GTATAAAGAG GAATGGGAGA AAAATAAGTC TAAAGTACAT 
GATGTTGGTG CGGACACACT GACCTTAAAA GCTGCCAAAG CCTCTAGAGA TCTGGCCAGC 
GATATCAAAT ATAAGGAATC GTTTGTAAAG AACAAGGACA AAGCAGTGGG TGTGAATGTG 
AGTGACTCCA AAACCCTCCA TTGTCTCCAA GTGGGGAAGC TGAACAGTGA GATTGCCTAT 
AAGAAGAATT CAAAGGAGAC TCAGGCTAAG TATAACCTGA CGAAAGACAT GGTGAATCTA 
AGCCATGCGA AGAAGGCCCA GGCCCTGGCC AGTGATCTGG ACTACAGGAA GAAGCTCCAC 
GAGTACACCA TCCTGCCTGA TGACATCAAA GTTCAGCAGG CTAAGAAGGC CTACAACCTC 
CAGAGTGATA ACCAGTACCG ATCTGACCTG GTCTGGATGA AGGGAGTGGG CTGGGAGGCT 
GAAGGCTGCC TGGATGTTGA ACAGGCCAAG AAGGCTGGAG AGCTTCTGAG CGATAAAAAG 
TATCGGCAGA AGGTGGACAG TGTAAAATTC ACCCAGGTGG CCGACACCCC ATCCATACAA 
CATGCCAAGA AGAGCCAGGA ACTACAGAGT GATCTGACAT ACAAGGCAGG TACAGAGCAG 
ATCATTCATC AGTACACTGT GAGCAAAGAC GAGCCTCTCT ACAAGCAGGC CAAAGCTAAC 
GCGGAGCTTC TCAGCAATAA ATCATATAAG AGTAGCTGGG AAGCCCAGAG GGAGAAGGGC 
TTTGAGCTGA GTATGGATGC ACTGTCTATC CTCACTGCCA AAGCTAAGAG GGATCTTGCC 
AGTGATGTGA AGTATAAGCA GCAGTATGAG ATGACAAAGG GGAAGATGAT AGGGGTGAAG 
AATGTAACAG ATGATTCTCA TTTGGCCCAC TCAGCTCTGG CCACTAAGCT CCAGAGTGAT 
CGCAACTATA AGAAAGAGTA CGAGGATACT AAGACCAAGC ACAGTGCCTC GCTTGATATG 
ATGACCATCA GCCATGCTAA AAAGGCCCAA GACCTGGCCA CAGAGACCAA CTACAGAACC 
ATCCTGCACG AGTACACCAC CCTGCCCAGT GATATGAAGG TGGCATGGGC CAAGAAAGCC 
TACGGCCAAC AAAGTGATAA AAAGTACCGC TCAGATCTGA ACTGGATGAA AGGTGTGGGC 
TGGGAGACCA CTGGATCTCT CGATGTGAAG CAGGCTAGGA AAGCTAGTGA TCTCGCCAGT 
GAGAAAAAGT ACCGCCAGCA TGTTAGTGCT CTGAAGTTCA CCAGCGTGGA GGACACGCCA 
GAGATGGTGC AGGCCAAACT CAGCAATAAG CTGGCACTAG ACAGGCTGTA CAGAGAGAAA 
GGAGAGACTG AGAAGCACAA GTACACAATG ACAGGAGATC TGCCAGAGCA CGTGCAGGCC 
AAGATGAACG CTATGAACAT CAGTGAGTCA CGATACAAAG AGTCATGGAT GAAGCTCAGA 
GATGGGGGCT ACAAGCTGCG CTTGGATGCT ATTCCATTCC AGGCTGCCAA ATCCTCAGGG 
GAGGTGCTCA GTGATCAAAA GTACAAAGAG GAGTTTCTGA AGATGAAGGG TAAGATGATC 
GGGCTGAAAG CACTCCAGGA TGACATCAAC ATCGCCCACT CTGTGCATGC CAGCCATCTA 
CAGAGTGATA TCAAATATAA GAAGGATTCT ACTAAGGTGC ACTCTCAGTT CCACCTACCG 
ATGGACATGC TGGAAGTAGC CCATGCTAAG AAGGCCCAGG CTCTGGTCAG CGATCAGGAC 
TATAGAATGA CCCTACACAA CTACACTACA CTGCCTGATG ACCTAAATGT GCAGGCTGCT 
AAGAGAGCTT ATTCTCTGCA GAGCGAGAAA GCCTACCGTT CTGATCTGAA TTACCTGCGT 
GGTGCGGCCT GGATTGCCAC CGGCGCCTTA CAAATCGAAG GCTCCAAACA TGCAACACAG 
CTGATCAGCG ATAAAAAGTA TAGACAGCAG CCGTACACAT TTAAGCACAC ATCAGTTACA 
GACTCTCCTG ACATAGTTCA CGCCAAGCTC AGCAACAAGC TCACCAACGA GCGTCTATAC 
AAAGAAAAGG GTGAGAACTT CAGGCATAAC TACACAATCA CAGCTGAGCG ACCAGAAATC 
ACCCAGGCCA AGATCAACGC TGCTAATTAC AGTGATACCA AGTACAAAGA GTCATGGCAC 
ACGCTGAGAG CACAAGGCTA TAAACTGACC ATGCAAGACA TCCCCTTCCA AGCAGCCAAA 
GCATCTACCG ACATTGCCAG TGATTACCAG TACAAGCACA ACCACGTCCT GGAAAAAGGC 
AAGCACATCG GAGCACGGAG CATCATGGAT GACTCTCACC TCCTGCACTG CATGCAGACG 
GCACATCTGC AGAGTGATAA GAATTACCGT AAAGATGCTA TTGCCACCAG CGGCCAGTTT 
CATCTGGGAA TGGACCTGGT TAACCTGGTC CACGCTAAGA AAGCCCAGTC TCTGGCCAGT 
GACCAGGACT ACAAGACCCG CCTGCATTCC TACACTGTCC TGCCTGATGA CATAAAGGTG 
CAGTGGGCCA AGAAGGCCTA TGAGCTGCAG AGTCAGAATG CGTACAAGTC TGATTTGAAC 
TTCATGAAGG GAGTGGCATG GGATTCAGTA GGAGCTCCTC AGCTGGAGTC TGCAAAGAAA 
GCAGGAGAGC TCATCAGCGA TAAGAAGTAT CGTCAGCTTC CTGACAGGCT GAAGTTTACC 
CAGGTTGCAG ACTCACCTGA TATCATTCAT GCTAAGAACA GCTACATGCA GTGTAGTGAG 
AGGCTGTATA AGTCTGGAGA TTCTGACTCC ATGCATAAGT ACACCTTACA TGCTGATCAC 
CCCGACTTTA TCAGAGCAAA GATGAACGCT CAGCAGATCA GTGATAAAGT CTACAAGGCA 
TCAGGAGATC AGGTGAAGAC TGCAGGATAT GACTTGAGGC TAGATGCCAT TCCCTTCCAG 
ACAGCCAAGG CCTCTAGAGA AATTGCAAGT GATTTCCGCT ATAAGGAAGC CTTTGTGAAG 
CAGAAGGGTC AGCAGGTGGG TCTGCTCAGT GTAGAGGACG ACCCCAAGAT GAGGCACTCG 
CTGGCTGTAG GCAAACTGCA GAGCGACAAT GAGTACAAGA AGCAGTTCCA GGAGACCCGC 
TCCCAGTATA AGATCCACGC AGACCAGCCT GAATTCCTGC AAGCAAGGAA GAGCCAGGCT 
CAGGCCAGTG ACCTCAGCTA CCGCCAACGC CTCCACAACT ACACATGTGA CCCAGAACAG 
CTCAACTTTA AACACGCCAA AGAGGCCTAC AAGCTGCAGA GTGATGTGAA ATATAAGTCA 
GACCTGAACT GGATAAAGGG AGTTGGCTGG ACTCCTCCGG GCTCTCATAA GGTAGAGATG 
GCTCGGAGGG CAGCAGAACT TGGCCTGGCT GATGGAGTGG ACACTGATGA AGCTATTGCG 
AAGTACCAGC AACTAATGAT GTTCCAGCGC GAGCAGCAGA TGCTAGAGCA GCAGCAGCAG 
CAGCAGGACT TCTCGGATCA GGTGGAAACT GAAGAACATG TCGAACAGTC TGTGGTCAAT 
CCAGATGCCA TGGAGATCCT CCATGTGAAG AGAAAGAAGA CCATCCACAC TACTTTCAAA 
CAAGCAAAGA CTATGGCATC GCAGTCTGTA ACATCTCACT CTTCTACAAA GCAGTCCACT 
GAGAGCCAGC ACAGGTCCAT TACAGAGAAA TCCACTGAAA ACAAGTCCAT AACGATGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

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1-877-436-7274

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nrap cDNA ORF clone, Astyanax mexicanus(Mexican tetra)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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