jag1a cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	jag1a
Entrez Gene ID	140421
Full Name	jagged 1a
Synonyms	cb243,jag1,sb:cb243,serrateC
General protein information	Preferred Names jagged 1a Names protein jagged-1a jagged1
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
NM_131861.1	NP_571936.1	protein jagged-1a precursor
XM_005168204.4	XP_005168261.1	protein jagged-1a isoform X1
XM_005168205.3	XP_005168262.1	protein jagged-1a isoform X2
XM_005168204.3	XP_005168261.1	protein jagged-1a isoform X1
XM_005168205.4	XP_005168262.1	protein jagged-1a isoform X2

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Wnt/β-catenin signaling controls intrahepatic biliary network formation in zebrafish by regulating notch activity.
So J, Khaliq M, Evason K, Ninov N, Martin BL, Stainier DYR, Shin D
Hepatology (Baltimore, Md.)67(6)2352-2366(2018 06)

Ultrafine Particle Exposure Reveals the Importance of FOXO1/Notch Activation Complex for Vascular Regeneration.
Baek KI, Packard RRS, Hsu JJ, Saffari A, Ma Z, Luu AP, Pietersen A, Yen H, Ren B, Ding Y, Sioutas C, Li R, Hsiai TK
Antioxidants & redox signaling28(13)1209-1223(2018 05)

itFISH: Enhanced Staining by Iterative Fluorescent In Situ Hybridization.
Row RH, Martin BL
Zebrafish14(6)578-580(2017 12)

Automated Segmentation of Light-Sheet Fluorescent Imaging to Characterize Experimental Doxorubicin-Induced Cardiac Injury and Repair.
Packard RRS, Baek KI, Beebe T, Jen N, Ding Y, Shi F, Fei P, Kang BJ, Chen PH, Gau J, Chen M, Tang JY, Shih YH, Ding Y, Li D, Xu X, Hsiai TK
Scientific reports7(1)8603(2017 08)

Zebrafish Caudal Haematopoietic Embryonic Stromal Tissue (CHEST) Cells Support Haematopoiesis.
Wolf A, Aggio J, Campbell C, Wright F, Marquez G, Traver D, Stachura DL
Scientific reports744644(2017 03)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following jag1a gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the jag1a cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ODa00351	NM_131861.1 Latest version!	Danio rerio jagged canonical Notch ligand 1a (jag1a), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
ODa06193	XM_005168204.4 Latest version!	Danio rerio jagged 1a (jag1a), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
ODa06194	XM_005168205.4 Latest version!	Danio rerio jagged 1a (jag1a), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
View Old Accession Versions >>
ODa06193	XM_005168204.3	Danio rerio jagged 1a (jag1a), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
ODa06194	XM_005168205.3	Danio rerio jagged 1a (jag1a), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
Hide Old Accession Versions >>

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

jag1a ( NM_131861.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_571936.1 Danio rerio jagged canonical Notch ligand 1a (jag1a), mRNA.

Price & Availability↑

CloneID	ODa00351
Clone ID Related Accession (Same CDS sequence)	NM_131861.1
Accession Version	NM_131861.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3729bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1577116800000
Organism	Danio rerio(zebrafish)
Product	protein jagged-1a precursor
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AF229448.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF229448.1, AY221107.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA2168446, SAMEA2168447 [ECO:0000350] ##Evidence-Data-END##

ATGATTCTCA GACCGAGCGC AACTTTTGCG GCGCTGTCCG CTCACGTGCT GCTGAGGTGC 
TTGTGGATGC GGGTATGTGA GGCTTCGGGG CATTTCGAGA TGCAGGTTTT GTCCATGCAG 
AATGTGAACG GGGAACTGCA GAGCGGCGCG TGCTGTGACG GTGCGCGTGA CCCGGCGGAG 
CGCTCCTGCG CGGCGGACCA GTGTGATACG TTTTTCCGCG TATGTTTGAA GGAGTATCAG 
TCGCGGGTGT CTTCGGGAGG CCCTTGCAGC TACGGAAGCG GATCTACTCC TGTAATTGGT 
GGGAATACTT TTTCTGTCAA GCCCCTTGAC CAAACAAACG ACAAAACCCG GATCGTGCTG 
CCCTTCAGCT TCGCGTGGCC GCGGTCGTAT ACGCTGATTG TGGAGGCGTT GGACTTCAAT 
AATGACTCGA GCACTGGCAG CATAAATGGA CAAGTGATCG AGAAAGCGGT GCAGTCGGGT 
ATGATCAACC CAAACCGTCA ATGGCAGGTC CTGAAACACA ACGGCCCCGT GGCTCAGTTC 
CAGTACCAGA TTCGGGTGAC CTGCGACGAG CACTACTTTG GCTTTGGCTG TAATAAGTTC 
TGTCGGCCCA GAGACGACTT TTTCGGCCAC TATACCTGCG ATCACAACGG AAACAAGACC 
TGTTTGGAAG GATGGGCCGG CCCAGAATGC AACACTGCTA TCTGTAAGCA GGGCTGCAGC 
ATCGAGCACG GCTCCTGTAA AGTGCCTGGA AACTGCAGGT GTCTGTACGG GTGGCAGGGT 
GAATACTGTG ATCAGTGTAT TCCTCATCCC GGATGTGTCC ATGGAACATG CATCGAACCC 
TGGCAGTGCC TGTGTGACAC CAACTGGGGA GGACAGCTCT GTGACAAAGA CTTGAACACC 
TGTGGCACAC TTCAGCCGTG TCTCAATGGT GGCACCTGCA GTAACACGGG ACCAGACAAA 
TACCACTGTG CCTGTCCTGA CGGCTACTCC GGACAGAACT GCGAGAGAGC TGATAACGCC 
TGTCTATCGG AGCCCTGTCT GAACGGAGGC CTCTGTGTGG AGTCCAGTCT GGGCTTTGAG 
TGTCAGTGTG CGGCCGGCTG GACGGGACCC TCCTGCAACA TCAATGAAGA CGACTGCAGT 
CCAAACCCGT GTAATCACAG CGGTGTGTGT GTGGACCTGG TGGACGGGTT TAAATGCATC 
TGCCCTGTAC AGTGGACTGG AAAGACCTGT CTCATCGATG CTAATGAGTG TGAGGAAAGC 
CCGTGCGTCA ATGCCCACTC CTGCCGCAAT CTGATTGGTG GATACTTCTG CGAGTGCCTG 
CCTGGCTGGA CGGGACAGAA TTGCGACATC AATGTCAACG ACTGCCACGG CCAGTGTCTG 
AACGGAGGTT TGTGTAAGGA TCTGGTGAAT GGATATCGGT GTGTGTGCGC AGCAGGGTTT 
GCAGGCGATC GCTGCGAGCG GGACGTGGAT GAGTGCGCAA GTCGCCCCTG TCTGAATGGC 
GGACGCTGCC AGGACACACT CAATGGATTC CAGTGTTTGT GCCCGCCTGG TTTCTCCGGT 
GCCACCTGCC AGCTGGATTT GGACTACTGT GAGAGCGGGC CGTGTCAGAA CGGGGCTCAG 
TGCTTCAGTC TGGCCTCAGA TTATTACTGT AAATGTCCAG AAGATTACGA AGGCAAGAAC 
TGCTCTCAGC TCAAAGACCA CTGCCTCATC ACACCCTGCC AAGTGATTGA CAGCTGTACT 
GTGGCGGTGG TGTCAAACAG CACCCCAGGC GGTCTGAGGC TAATCTCTTC CAGTGTGTGC 
GGTCCACACG GCCGCTGCCG TAGCCACAGC CACGCCGGAG GACACTTCAG CTGCGACTGT 
CAAGACGGCT TCACCGGGAC CTACTGCCAC GAGAACATCA ATGACTGTGA AAGCAGCCCC 
TGTCTCAGCG GAGGCACTTG CATTGACAAA ATCAATGCAT ATCAGTGTAT CTGTGCTGAC 
GGATGGGAAG GACCAAACTG CGAGACCAAC ATTGACGACT GCCGCACGAA CCCATGTCGA 
GATCGAGGAG TGTGTCGTGA CCTGGTCAAT GACTTTTACT GCGAGTGTGA GAACGGCTGG 
AAGGGAAAGA CCTGTCACTC ACGCGAGAGT CAATGCGATG AAGACACATG CAACAACGGA 
GGAACATGTT CAGATGAAGG CGACTCGTTC AAGTGCTTGT GTTCACCTGG ATGGGAAGGA 
GCAACGTGTA ATATAGCCAA GAACAGCAGC TGCCTGCCGA ACCCGTGTGA GAACGGAGCC 
ACGTGTGTGG TGACAGGCGA CGGCTTCACC TGCGTCTGCA AAGAAGGCTG GGAAGGGCCC 
ACCTGCAGCC AGAATTCCAA TGATTGCAAC CCACAACCAT GTTATAACAG TGGGACATGT 
GTGGATGGGG ATAACTGGTA CCGCTGTGAG TGCGCCAGTG GCTTTGCAGG ACCAGACTGC 
CGCATCAACA TCAATGAGTG CCAGTCGTCA CCCTGTGCGT TCGGCTCTAC CTGTGTGGAT 
GAAATTAATG GCTACCGCTG CTTGTGTCCA CCAGGCAGAA CCGGACCCAG ATGTCAGGAA 
GTCACAGGAC GCCCGTGTGT GATTGGCGGT CGGATTGCTG TTGATGGAGC AAAGTGGGCT 
GAAGACTGCA ATACTTGCTA CTGCCACAAA GGCATTGTCA CATGTACTAA GCTGTTCTGT 
GGCCCTAAAG CATGCCGTAT GCTTGGCTCG GGTCGAGGGG ACTGTCCTAC GGGTCAGCTG 
TGTGTGCCGG TCCGCGATGA GCAGTGTTTC GTCAAGCCCT GTTCCTCCCA GGGCGAGTGC 
TGGTCTGCTC ACCGGCCGGC CGTACGCACA CACTGCCAAC CTGACAGCCA CTGCGCAAAC 
GTCACCTTCA CCTTCAACAA GGACACCATG CCACAGGGGG TGACGGTGGA GCAGGTTTGT 
CGTGAACTCA GGCATCTTTA TGTGACTAAA AATGTTACAT CAGAGTTTTC CATCTCTGTG 
AGCTGTGAAC TCTCTTCTGC CGCCAGTAAC GAGATCCATG TGGCCATTCA TGTGACTGAG 
AACGGCATTC ATGGCCGGGT TCCTGTTAAG GAAATTACAG ACAACATCAT TGATCTGGTG 
AGCAAACACA GTGCTAACAG CTCCGTCATC GGCTCCATCG CTGAGGTTCG AGTGCAGCGC 
AAACAACCGC AAAACCCCAA CGTAGACTAC ATGGTGCCGC TGCTAGTTTC GGTCGTGACT 
GCAATCTGGG TTTTGGCCCT GGCGTCAGTG TTCCTGTGGT GCATCCGTCA TCACCGTAAA 
CAAAGCTCCA GCGCCACCGC CATCAACCCT ACCAGCCCCT TCTCCACACC TGAAGAAAAC 
ACAGCGAACA ACGCCCGCGA GCATCTCAAC CAGATCAAGA ACCACATTGA GAAAAATGCC 
TCAAACGGCA GCCTGCCAGG AAAAGAGTTG CACTGTGACG ACAAAAACAC AGTCAACGCC 
AAAATCAGGA CGCAATTCCC TGAATCTGAC GCCAGTAGAC GACTACAAAA AACACGGTTT 
CCACATCAGC CAGCTTATAT GCTGGTGGAC CGAGATGATA GGTTGAGCTC CAATGGCACA 
GACATAAAGA AACACCCCCA ATGGACAAAT AAACGCGACA ATAGAGACCT TGAGTCGCAG 
CACCGGGTGC CAGATTCACA GCACAGAGAC TCGCAACACA GCTTGCAGAA AATGGAGTAT 
ATCGTATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_571936.1
CDS	207..3935
Translation	MILRPSATFAALSAHVLLRCLWMRVCEASGHFEMQVLSMQNVNGELQSGACCDGARDPAERSCAADQCDTFFRVCLKEYQSRVSSGGPCSYGSGSTPVIGGNTFSVKPLDQTNDKTRIVLPFSFAWPRSYTLIVEALDFNNDSSTGSINGQVIEKAVQSGMINPNRQWQVLKHNGPVAQFQYQIRVTCDEHYFGFGCNKFCRPRDDFFGHYTCDHNGNKTCLEGWAGPECNTAICKQGCSIEHGSCKVPGNCRCLYGWQGEYCDQCIPHPGCVHGTCIEPWQCLCDTNWGGQLCDKDLNTCGTLQPCLNGGTCSNTGPDKYHCACPDGYSGQNCERADNACLSEPCLNGGLCVESSLGFECQCAAGWTGPSCNINEDDCSPNPCNHSGVCVDLVDGFKCICPVQWTGKTCLIDANECEESPCVNAHSCRNLIGGYFCECLPGWTGQNCDINVNDCHGQCLNGGLCKDLVNGYRCVCAAGFAGDRCERDVDECASRPCLNGGRCQDTLNGFQCLCPPGFSGATCQLDLDYCESGPCQNGAQCFSLASDYYCKCPEDYEGKNCSQLKDHCLITPCQVIDSCTVAVVSNSTPGGLRLISSSVCGPHGRCRSHSHAGGHFSCDCQDGFTGTYCHENINDCESSPCLSGGTCIDKINAYQCICADGWEGPNCETNIDDCRTNPCRDRGVCRDLVNDFYCECENGWKGKTCHSRESQCDEDTCNNGGTCSDEGDSFKCLCSPGWEGATCNIAKNSSCLPNPCENGATCVVTGDGFTCVCKEGWEGPTCSQNSNDCNPQPCYNSGTCVDGDNWYRCECASGFAGPDCRININECQSSPCAFGSTCVDEINGYRCLCPPGRTGPRCQEVTGRPCVIGGRIAVDGAKWAEDCNTCYCHKGIVTCTKLFCGPKACRMLGSGRGDCPTGQLCVPVRDEQCFVKPCSSQGECWSAHRPAVRTHCQPDSHCANVTFTFNKDTMPQGVTVEQVCRELRHLYVTKNVTSEFSISVSCELSSAASNEIHVAIHVTENGIHGRVPVKEITDNIIDLVSKHSANSSVIGSIAEVRVQRKQPQNPNVDYMVPLLVSVVTAIWVLALASVFLWCIRHHRKQSSSATAINPTSPFSTPEENTANNAREHLNQIKNHIEKNASNGSLPGKELHCDDKNTVNAKIRTQFPESDASRRLQKTRFPHQPAYMLVDRDDRLSSNGTDIKKHPQWTNKRDNRDLESQHRVPDSQHRDSQHSLQKMEYIV

Target ORF information:

RefSeq Version	NM_131861.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio jagged canonical Notch ligand 1a (jag1a), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_131861.1

ORF Insert Sequence:

ATGATTCTCA GACCGAGCGC AACTTTTGCG GCGCTGTCCG CTCACGTGCT GCTGAGGTGC 
TTGTGGATGC GGGTATGTGA GGCTTCGGGG CATTTCGAGA TGCAGGTTTT GTCCATGCAG 
AATGTGAACG GGGAACTGCA GAGCGGCGCG TGCTGTGACG GTGCGCGTGA CCCGGCGGAG 
CGCTCCTGCG CGGCGGACCA GTGTGATACG TTTTTCCGCG TATGTTTGAA GGAGTATCAG 
TCGCGGGTGT CTTCGGGAGG CCCTTGCAGC TACGGAAGCG GATCTACTCC TGTAATTGGT 
GGGAATACTT TTTCTGTCAA GCCCCTTGAC CAAACAAACG ACAAAACCCG GATCGTGCTG 
CCCTTCAGCT TCGCGTGGCC GCGGTCGTAT ACGCTGATTG TGGAGGCGTT GGACTTCAAT 
AATGACTCGA GCACTGGCAG CATAAATGGA CAAGTGATCG AGAAAGCGGT GCAGTCGGGT 
ATGATCAACC CAAACCGTCA ATGGCAGGTC CTGAAACACA ACGGCCCCGT GGCTCAGTTC 
CAGTACCAGA TTCGGGTGAC CTGCGACGAG CACTACTTTG GCTTTGGCTG TAATAAGTTC 
TGTCGGCCCA GAGACGACTT TTTCGGCCAC TATACCTGCG ATCACAACGG AAACAAGACC 
TGTTTGGAAG GATGGGCCGG CCCAGAATGC AACACTGCTA TCTGTAAGCA GGGCTGCAGC 
ATCGAGCACG GCTCCTGTAA AGTGCCTGGA AACTGCAGGT GTCTGTACGG GTGGCAGGGT 
GAATACTGTG ATCAGTGTAT TCCTCATCCC GGATGTGTCC ATGGAACATG CATCGAACCC 
TGGCAGTGCC TGTGTGACAC CAACTGGGGA GGACAGCTCT GTGACAAAGA CTTGAACACC 
TGTGGCACAC TTCAGCCGTG TCTCAATGGT GGCACCTGCA GTAACACGGG ACCAGACAAA 
TACCACTGTG CCTGTCCTGA CGGCTACTCC GGACAGAACT GCGAGAGAGC TGATAACGCC 
TGTCTATCGG AGCCCTGTCT GAACGGAGGC CTCTGTGTGG AGTCCAGTCT GGGCTTTGAG 
TGTCAGTGTG CGGCCGGCTG GACGGGACCC TCCTGCAACA TCAATGAAGA CGACTGCAGT 
CCAAACCCGT GTAATCACAG CGGTGTGTGT GTGGACCTGG TGGACGGGTT TAAATGCATC 
TGCCCTGTAC AGTGGACTGG AAAGACCTGT CTCATCGATG CTAATGAGTG TGAGGAAAGC 
CCGTGCGTCA ATGCCCACTC CTGCCGCAAT CTGATTGGTG GATACTTCTG CGAGTGCCTG 
CCTGGCTGGA CGGGACAGAA TTGCGACATC AATGTCAACG ACTGCCACGG CCAGTGTCTG 
AACGGAGGTT TGTGTAAGGA TCTGGTGAAT GGATATCGGT GTGTGTGCGC AGCAGGGTTT 
GCAGGCGATC GCTGCGAGCG GGACGTGGAT GAGTGCGCAA GTCGCCCCTG TCTGAATGGC 
GGACGCTGCC AGGACACACT CAATGGATTC CAGTGTTTGT GCCCGCCTGG TTTCTCCGGT 
GCCACCTGCC AGCTGGATTT GGACTACTGT GAGAGCGGGC CGTGTCAGAA CGGGGCTCAG 
TGCTTCAGTC TGGCCTCAGA TTATTACTGT AAATGTCCAG AAGATTACGA AGGCAAGAAC 
TGCTCTCAGC TCAAAGACCA CTGCCTCATC ACACCCTGCC AAGTGATTGA CAGCTGTACT 
GTGGCGGTGG TGTCAAACAG CACCCCAGGC GGTCTGAGGC TAATCTCTTC CAGTGTGTGC 
GGTCCACACG GCCGCTGCCG TAGCCACAGC CACGCCGGAG GACACTTCAG CTGCGACTGT 
CAAGACGGCT TCACCGGGAC CTACTGCCAC GAGAACATCA ATGACTGTGA AAGCAGCCCC 
TGTCTCAGCG GAGGCACTTG CATTGACAAA ATCAATGCAT ATCAGTGTAT CTGTGCTGAC 
GGATGGGAAG GACCAAACTG CGAGACCAAC ATTGACGACT GCCGCACGAA CCCATGTCGA 
GATCGAGGAG TGTGTCGTGA CCTGGTCAAT GACTTTTACT GCGAGTGTGA GAACGGCTGG 
AAGGGAAAGA CCTGTCACTC ACGCGAGAGT CAATGCGATG AAGACACATG CAACAACGGA 
GGAACATGTT CAGATGAAGG CGACTCGTTC AAGTGCTTGT GTTCACCTGG ATGGGAAGGA 
GCAACGTGTA ATATAGCCAA GAACAGCAGC TGCCTGCCGA ACCCGTGTGA GAACGGAGCC 
ACGTGTGTGG TGACAGGCGA CGGCTTCACC TGCGTCTGCA AAGAAGGCTG GGAAGGGCCC 
ACCTGCAGCC AGAATTCCAA TGATTGCAAC CCACAACCAT GTTATAACAG TGGGACATGT 
GTGGATGGGG ATAACTGGTA CCGCTGTGAG TGCGCCAGTG GCTTTGCAGG ACCAGACTGC 
CGCATCAACA TCAATGAGTG CCAGTCGTCA CCCTGTGCGT TCGGCTCTAC CTGTGTGGAT 
GAAATTAATG GCTACCGCTG CTTGTGTCCA CCAGGCAGAA CCGGACCCAG ATGTCAGGAA 
GTCACAGGAC GCCCGTGTGT GATTGGCGGT CGGATTGCTG TTGATGGAGC AAAGTGGGCT 
GAAGACTGCA ATACTTGCTA CTGCCACAAA GGCATTGTCA CATGTACTAA GCTGTTCTGT 
GGCCCTAAAG CATGCCGTAT GCTTGGCTCG GGTCGAGGGG ACTGTCCTAC GGGTCAGCTG 
TGTGTGCCGG TCCGCGATGA GCAGTGTTTC GTCAAGCCCT GTTCCTCCCA GGGCGAGTGC 
TGGTCTGCTC ACCGGCCGGC CGTACGCACA CACTGCCAAC CTGACAGCCA CTGCGCAAAC 
GTCACCTTCA CCTTCAACAA GGACACCATG CCACAGGGGG TGACGGTGGA GCAGGTTTGT 
CGTGAACTCA GGCATCTTTA TGTGACTAAA AATGTTACAT CAGAGTTTTC CATCTCTGTG 
AGCTGTGAAC TCTCTTCTGC CGCCAGTAAC GAGATCCATG TGGCCATTCA TGTGACTGAG 
AACGGCATTC ATGGCCGGGT TCCTGTTAAG GAAATTACAG ACAACATCAT TGATCTGGTG 
AGCAAACACA GTGCTAACAG CTCCGTCATC GGCTCCATCG CTGAGGTTCG AGTGCAGCGC 
AAACAACCGC AAAACCCCAA CGTAGACTAC ATGGTGCCGC TGCTAGTTTC GGTCGTGACT 
GCAATCTGGG TTTTGGCCCT GGCGTCAGTG TTCCTGTGGT GCATCCGTCA TCACCGTAAA 
CAAAGCTCCA GCGCCACCGC CATCAACCCT ACCAGCCCCT TCTCCACACC TGAAGAAAAC 
ACAGCGAACA ACGCCCGCGA GCATCTCAAC CAGATCAAGA ACCACATTGA GAAAAATGCC 
TCAAACGGCA GCCTGCCAGG AAAAGAGTTG CACTGTGACG ACAAAAACAC AGTCAACGCC 
AAAATCAGGA CGCAATTCCC TGAATCTGAC GCCAGTAGAC GACTACAAAA AACACGGTTT 
CCACATCAGC CAGCTTATAT GCTGGTGGAC CGAGATGATA GGTTGAGCTC CAATGGCACA 
GACATAAAGA AACACCCCCA ATGGACAAAT AAACGCGACA ATAGAGACCT TGAGTCGCAG 
CACCGGGTGC CAGATTCACA GCACAGAGAC TCGCAACACA GCTTGCAGAA AATGGAGTAT 
ATCGTATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

jag1a ( XM_005168204.4 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005168261.1 Danio rerio jagged 1a (jag1a), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa06193
Clone ID Related Accession (Same CDS sequence)	XM_005168204.4 , XM_005168204.3
Accession Version	XM_005168204.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3762bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1497369600000
Organism	Danio rerio(zebrafish)
Product	protein jagged-1a isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007112.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_005168204.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATTCTCA GACCGAGCTC AACTTTTGCG GCGCTGTCCG CTCACGTGCT GCTGAGGTGC 
TTGTGGATGC GGGTATGTGA GGCTTCGGGG CATTTCGAGA TGCAGGTTTT GTCCATGCAG 
AATGTGAACG GGGAGCTGCA GAACGGCGCG TGCTGTGACG GTGCGCGTGA CCCGGCGGAG 
CGCTCCTGCG CGGCGGACCA GTGTGATACG TTTTTCCGCG TATGTTTGAA GGAGTATCAG 
TCGCGGGTGT CTTCGGGAGG CCCTTGCAGC TACGGAAGCG GATCTACTCC TGTAATTGGT 
GGGAATACTT TTTCTGTCAA GCCCCTTGAC CAAACAAATG ACAAAACCCG GATCGTGCTG 
CCCTTCAGCT TCGCGTGGCC GCGGTCGTAT ACGCTGATTG TGGAGGCGTT GGACTTCAAT 
AATGACTCAA GCACTGGCAG CATAAATGGA CAAGTGATCG AGAAAGCGGT GCAGTCGGGC 
ATGATCAACC CAAACCGTCA ATGGCAGGTC CTGAAGCACA ACGGCCCCGT GGCTCAGTTC 
CAGTACCAAA TTCGGGTGAC CTGCGACGAG CACTACTTTG GCTTTGGCTG TAATAAGTTC 
TGTCGGCCCA GAGACGACTT TTTCGGCCAC TATACCTGCG ATCACAACGG AAACAAGACC 
TGTTTGGAAG GATGGGCCGG CCCAGAATGC AACACTGCTA TCTGTAAGCA GGGCTGCAGC 
ATCGAGCACG GCTCCTGTAA AGTGCCTGGA AACTGCAGGT GTCTGTACGG GTGGCAGGGT 
GAATACTGTG ATCAGTGTAT TCCTCATCCC GGATGTGTCC ATGGAACATG CATCGAACCC 
TGGCAGTGCC TGTGTGACAC CAACTGGGGA GGACAGCTCT GTGACAAAGA CTTAAACACC 
TGTGGCACAC TTCAGCCGTG TCTCAATGGT GGCACCTGCA GTAACACGGG ACCAGACAAA 
TACCACTGCG CCTGTCCTGA GGGCTACTCC GGACAGAACT GCGAGAGAGC TGATAACGCC 
TGTCTATCGG AGCCCTGTCT GAACGGAGGC CTCTGTGTGG AGTCCAGTCT GGGCTTTGAG 
TGTCAGTGTG CGGCCGGCTG GACGGGACCC TCCTGCAACA TCAATGAAGA CGACTGCAGT 
CCAAACCCGT GTAATCACAG CGGTGTGTGT GTGGACCTGG TGGACGGGTT CAAATGCATC 
TGCCCCGTGC AGTGGACTGG AAAGACCTGT CTCATCGATG CTAATGAGTG TGAGGAAAGC 
CCGTGCGTCA ATGCCCACTC CTGCCGCAAT CTGATTGGTG GATACTTCTG CGAGTGCCTG 
CCTGGCTGGA CGGGACAGAA TTGCGACATC AATGTCAACG ACTGCCACGG CCAGTGTCTG 
AACGGAGGTT TGTGTAAGGA TCTGGTGAAT GGATATCGGT GTGTGTGCGC AGCAGGGTTT 
GCAGGCGATC GCTGCGAGCG GGACGTGGAT GAGTGCGCCA GTCGCCCCTG TCTGAATGGC 
GGACGCTGCC AGGACACACT CAATGGATTC CAGTGTTTGT GCCCGCCTGG TTTCTCCGGT 
GCCACCTGCC AGCTGGATTT GGACTACTGT GAGAGCGGGC CGTGTCAGAA CGGGGCTCAG 
TGCTTCAGTC TGGCCTCAGA TTATTACTGT AAATGTCCAG AAGATTACGA AGGCAAGAAC 
TGCTCTCAGC TCAAAGACCA CTGCCTCATC ACACCCTGCC AAGTGATTGA CAGCTGTACG 
GTGGCGGTGG TGTCAAACAG CACCCCAGGC GGTCTGAGGC TAATCTCTTC CAGTGTGTGC 
GGTCCACACG GCCGCTGCCG TAGCCACAGC CATGCCGGAG GACACTTCAG CTGCGACTGT 
CAAGACGGCT TCACCGGGAC CTACTGCCAC GAGAACATCA ATGACTGTGA AAGCAGCCCC 
TGTCTCAGCG GAGGCACTTG CATTGACAAA ATCAATGCAT ATCAGTGTAT CTGTGCTGAC 
GGATGGGAAG GACCAAACTG CGAGACCAAC ATTGACGACT GCCGCACGAA CCCATGTCGA 
GATCGAGGAG TGTGTCGTGA CCTGGTCAAT GACTTTTACT GCGAGTGTGA GAACGGCTGG 
AAGGGAAAGA CCTGTCACTC ACGCGAGAGT CAATGCGATG AAGACACATG CAACAACGGA 
GGAACATGTT CAGATGAAGG CGACTCGTTC AAGTGCTTGT GTTCACCCGG ATGGGAAGGA 
GCAACGTGTA ATATAGCCAA GAACAGCAGC TGCCTGCCGA ACCCGTGTGA GAACGGAGCC 
ACGTGTGTGG TGACAGGCGA CGGCTTCACC TGCGTCTGCA AAGAAGGCTG GGAAGGGCCC 
ACCTGCAGCC AGAATTCCAA TGATTGCAAC CCACAACCAT GTTATAACAG TGGGACATGT 
GTGGATGGGG ATAACTGGTA CCGCTGTGAG TGCGCCAGTG GCTTTGCAGG ACCAGACTGC 
CGCATCAACA TCAATGAGTG CCAGTCGTCA CCCTGTGCGT TCGGCTCTAC CTGTGTGGAT 
GAAATTAATG GCTACCGCTG CTTGTGTCCA CCAGGCAGAA CCGGACCCAG ATGTCAGGAA 
GTCACAGGAC GCCCGTGTGT GATTGGCGGT CGGATTGCTG TTGATGGAGC AAAGTGGGCT 
GAAGACTGCA ATACTTGCTA CTGCCACAAA GGCATTGTCA CATGTACTAA GCTGTTCTGT 
GGCCCTAAAG CATGCCGTAT GCTTGGCTCG GGTCGAGGGG ACTGTCCTAC GGGTCAGATG 
TGTGTGCCGG TCCGCGATGA GCAGTGTTTC GTCAAGCCCT GTTCCTCCCA GGGCGAGTGT 
TTCGTCAAGC CCTGTTCCTC CCAGGGCGAG TGCTGGTCTG CTCACCGGCC GACCGTACGC 
ACACACTGCC AACCTGACAG CCACTGCGCA AACGTCACCT TCACCTTCAA CAAGGACACC 
ATGCCACAGG GGGTGACGGT GGAGCAGGTT TGTCGTGAAC TCAGGCATCT TTATGTGACT 
AAAAATGTTA CATCAGAGTT TTCCATCTCT GTGAGCTGTG AACTCTCTTC TGCCGCCAGT 
AACGAGATCC ATGTGGCCAT TCATGTGACT GAGAACGGCA TTCATGGCCG GGTTCCTGTT 
AAGGAAATTA CAGACAACAT CATTGATCTG GTGAGCAAAC ACAGTGCTAA CAGCTCCGTC 
ATCGGCTCCA TCGCTGAGGT TCGAGTGCAG CGCAAACAAC CGCAAAACCC CAACGTAGAC 
TACATGGTGC CGCTGCTAGT TTCGGTCGTG ACTGCAATCT GGGTTTTGGC CCTGGCGTCA 
GTGTTCCTGT GGTGCATCCG TCATCACCGT AAACAAAGCT CCAGCGCCAC CGCCATCAAC 
CCTACCAGCC CCTTCTCCAC ACCTGAAGAA AACACAGCGA ACAACGCCCG CGAGCATCTC 
AACCAGATCA AGAACCACAT TGAGAAAAAT GCCTCAAACG GCAGCCTGCC AGGAAAAGAG 
TTGCACTGTG ACGACAAAAA CACAGTCAAC GCCAAAATCA GGACGCAATT CCCTGAATCT 
GACGCCAGTA GACGACTACA AAAAACACGG TTTCCACATC AGCCAGCTTA TATGCTGGTG 
GACCGAGATG ATAGGTTGAG CTCCAATGGC ACAGACATAA AGAAACACCC CCAATGGACA 
AATAAACGCG ACAATAGAGA CCTTGAGTCG CAGCACCGGG TGCCAGATTC ACAGCACAGA 
GACTCGCAAC ACAGCTTGCA GAAAATGGAG TATATCGTAT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005168261.1
CDS	251..4012
Translation	MILRPSSTFAALSAHVLLRCLWMRVCEASGHFEMQVLSMQNVNGELQNGACCDGARDPAERSCAADQCDTFFRVCLKEYQSRVSSGGPCSYGSGSTPVIGGNTFSVKPLDQTNDKTRIVLPFSFAWPRSYTLIVEALDFNNDSSTGSINGQVIEKAVQSGMINPNRQWQVLKHNGPVAQFQYQIRVTCDEHYFGFGCNKFCRPRDDFFGHYTCDHNGNKTCLEGWAGPECNTAICKQGCSIEHGSCKVPGNCRCLYGWQGEYCDQCIPHPGCVHGTCIEPWQCLCDTNWGGQLCDKDLNTCGTLQPCLNGGTCSNTGPDKYHCACPEGYSGQNCERADNACLSEPCLNGGLCVESSLGFECQCAAGWTGPSCNINEDDCSPNPCNHSGVCVDLVDGFKCICPVQWTGKTCLIDANECEESPCVNAHSCRNLIGGYFCECLPGWTGQNCDINVNDCHGQCLNGGLCKDLVNGYRCVCAAGFAGDRCERDVDECASRPCLNGGRCQDTLNGFQCLCPPGFSGATCQLDLDYCESGPCQNGAQCFSLASDYYCKCPEDYEGKNCSQLKDHCLITPCQVIDSCTVAVVSNSTPGGLRLISSSVCGPHGRCRSHSHAGGHFSCDCQDGFTGTYCHENINDCESSPCLSGGTCIDKINAYQCICADGWEGPNCETNIDDCRTNPCRDRGVCRDLVNDFYCECENGWKGKTCHSRESQCDEDTCNNGGTCSDEGDSFKCLCSPGWEGATCNIAKNSSCLPNPCENGATCVVTGDGFTCVCKEGWEGPTCSQNSNDCNPQPCYNSGTCVDGDNWYRCECASGFAGPDCRININECQSSPCAFGSTCVDEINGYRCLCPPGRTGPRCQEVTGRPCVIGGRIAVDGAKWAEDCNTCYCHKGIVTCTKLFCGPKACRMLGSGRGDCPTGQMCVPVRDEQCFVKPCSSQGECFVKPCSSQGECWSAHRPTVRTHCQPDSHCANVTFTFNKDTMPQGVTVEQVCRELRHLYVTKNVTSEFSISVSCELSSAASNEIHVAIHVTENGIHGRVPVKEITDNIIDLVSKHSANSSVIGSIAEVRVQRKQPQNPNVDYMVPLLVSVVTAIWVLALASVFLWCIRHHRKQSSSATAINPTSPFSTPEENTANNAREHLNQIKNHIEKNASNGSLPGKELHCDDKNTVNAKIRTQFPESDASRRLQKTRFPHQPAYMLVDRDDRLSSNGTDIKKHPQWTNKRDNRDLESQHRVPDSQHRDSQHSLQKMEYIV

Target ORF information:

RefSeq Version	XM_005168204.4
Organism	Danio rerio(zebrafish)
Definition	Danio rerio jagged 1a (jag1a), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005168204.4

ORF Insert Sequence:

ATGATTCTCA GACCGAGCTC AACTTTTGCG GCGCTGTCCG CTCACGTGCT GCTGAGGTGC 
TTGTGGATGC GGGTATGTGA GGCTTCGGGG CATTTCGAGA TGCAGGTTTT GTCCATGCAG 
AATGTGAACG GGGAGCTGCA GAACGGCGCG TGCTGTGACG GTGCGCGTGA CCCGGCGGAG 
CGCTCCTGCG CGGCGGACCA GTGTGATACG TTTTTCCGCG TATGTTTGAA GGAGTATCAG 
TCGCGGGTGT CTTCGGGAGG CCCTTGCAGC TACGGAAGCG GATCTACTCC TGTAATTGGT 
GGGAATACTT TTTCTGTCAA GCCCCTTGAC CAAACAAATG ACAAAACCCG GATCGTGCTG 
CCCTTCAGCT TCGCGTGGCC GCGGTCGTAT ACGCTGATTG TGGAGGCGTT GGACTTCAAT 
AATGACTCAA GCACTGGCAG CATAAATGGA CAAGTGATCG AGAAAGCGGT GCAGTCGGGC 
ATGATCAACC CAAACCGTCA ATGGCAGGTC CTGAAGCACA ACGGCCCCGT GGCTCAGTTC 
CAGTACCAAA TTCGGGTGAC CTGCGACGAG CACTACTTTG GCTTTGGCTG TAATAAGTTC 
TGTCGGCCCA GAGACGACTT TTTCGGCCAC TATACCTGCG ATCACAACGG AAACAAGACC 
TGTTTGGAAG GATGGGCCGG CCCAGAATGC AACACTGCTA TCTGTAAGCA GGGCTGCAGC 
ATCGAGCACG GCTCCTGTAA AGTGCCTGGA AACTGCAGGT GTCTGTACGG GTGGCAGGGT 
GAATACTGTG ATCAGTGTAT TCCTCATCCC GGATGTGTCC ATGGAACATG CATCGAACCC 
TGGCAGTGCC TGTGTGACAC CAACTGGGGA GGACAGCTCT GTGACAAAGA CTTAAACACC 
TGTGGCACAC TTCAGCCGTG TCTCAATGGT GGCACCTGCA GTAACACGGG ACCAGACAAA 
TACCACTGCG CCTGTCCTGA GGGCTACTCC GGACAGAACT GCGAGAGAGC TGATAACGCC 
TGTCTATCGG AGCCCTGTCT GAACGGAGGC CTCTGTGTGG AGTCCAGTCT GGGCTTTGAG 
TGTCAGTGTG CGGCCGGCTG GACGGGACCC TCCTGCAACA TCAATGAAGA CGACTGCAGT 
CCAAACCCGT GTAATCACAG CGGTGTGTGT GTGGACCTGG TGGACGGGTT CAAATGCATC 
TGCCCCGTGC AGTGGACTGG AAAGACCTGT CTCATCGATG CTAATGAGTG TGAGGAAAGC 
CCGTGCGTCA ATGCCCACTC CTGCCGCAAT CTGATTGGTG GATACTTCTG CGAGTGCCTG 
CCTGGCTGGA CGGGACAGAA TTGCGACATC AATGTCAACG ACTGCCACGG CCAGTGTCTG 
AACGGAGGTT TGTGTAAGGA TCTGGTGAAT GGATATCGGT GTGTGTGCGC AGCAGGGTTT 
GCAGGCGATC GCTGCGAGCG GGACGTGGAT GAGTGCGCCA GTCGCCCCTG TCTGAATGGC 
GGACGCTGCC AGGACACACT CAATGGATTC CAGTGTTTGT GCCCGCCTGG TTTCTCCGGT 
GCCACCTGCC AGCTGGATTT GGACTACTGT GAGAGCGGGC CGTGTCAGAA CGGGGCTCAG 
TGCTTCAGTC TGGCCTCAGA TTATTACTGT AAATGTCCAG AAGATTACGA AGGCAAGAAC 
TGCTCTCAGC TCAAAGACCA CTGCCTCATC ACACCCTGCC AAGTGATTGA CAGCTGTACG 
GTGGCGGTGG TGTCAAACAG CACCCCAGGC GGTCTGAGGC TAATCTCTTC CAGTGTGTGC 
GGTCCACACG GCCGCTGCCG TAGCCACAGC CATGCCGGAG GACACTTCAG CTGCGACTGT 
CAAGACGGCT TCACCGGGAC CTACTGCCAC GAGAACATCA ATGACTGTGA AAGCAGCCCC 
TGTCTCAGCG GAGGCACTTG CATTGACAAA ATCAATGCAT ATCAGTGTAT CTGTGCTGAC 
GGATGGGAAG GACCAAACTG CGAGACCAAC ATTGACGACT GCCGCACGAA CCCATGTCGA 
GATCGAGGAG TGTGTCGTGA CCTGGTCAAT GACTTTTACT GCGAGTGTGA GAACGGCTGG 
AAGGGAAAGA CCTGTCACTC ACGCGAGAGT CAATGCGATG AAGACACATG CAACAACGGA 
GGAACATGTT CAGATGAAGG CGACTCGTTC AAGTGCTTGT GTTCACCCGG ATGGGAAGGA 
GCAACGTGTA ATATAGCCAA GAACAGCAGC TGCCTGCCGA ACCCGTGTGA GAACGGAGCC 
ACGTGTGTGG TGACAGGCGA CGGCTTCACC TGCGTCTGCA AAGAAGGCTG GGAAGGGCCC 
ACCTGCAGCC AGAATTCCAA TGATTGCAAC CCACAACCAT GTTATAACAG TGGGACATGT 
GTGGATGGGG ATAACTGGTA CCGCTGTGAG TGCGCCAGTG GCTTTGCAGG ACCAGACTGC 
CGCATCAACA TCAATGAGTG CCAGTCGTCA CCCTGTGCGT TCGGCTCTAC CTGTGTGGAT 
GAAATTAATG GCTACCGCTG CTTGTGTCCA CCAGGCAGAA CCGGACCCAG ATGTCAGGAA 
GTCACAGGAC GCCCGTGTGT GATTGGCGGT CGGATTGCTG TTGATGGAGC AAAGTGGGCT 
GAAGACTGCA ATACTTGCTA CTGCCACAAA GGCATTGTCA CATGTACTAA GCTGTTCTGT 
GGCCCTAAAG CATGCCGTAT GCTTGGCTCG GGTCGAGGGG ACTGTCCTAC GGGTCAGATG 
TGTGTGCCGG TCCGCGATGA GCAGTGTTTC GTCAAGCCCT GTTCCTCCCA GGGCGAGTGT 
TTCGTCAAGC CCTGTTCCTC CCAGGGCGAG TGCTGGTCTG CTCACCGGCC GACCGTACGC 
ACACACTGCC AACCTGACAG CCACTGCGCA AACGTCACCT TCACCTTCAA CAAGGACACC 
ATGCCACAGG GGGTGACGGT GGAGCAGGTT TGTCGTGAAC TCAGGCATCT TTATGTGACT 
AAAAATGTTA CATCAGAGTT TTCCATCTCT GTGAGCTGTG AACTCTCTTC TGCCGCCAGT 
AACGAGATCC ATGTGGCCAT TCATGTGACT GAGAACGGCA TTCATGGCCG GGTTCCTGTT 
AAGGAAATTA CAGACAACAT CATTGATCTG GTGAGCAAAC ACAGTGCTAA CAGCTCCGTC 
ATCGGCTCCA TCGCTGAGGT TCGAGTGCAG CGCAAACAAC CGCAAAACCC CAACGTAGAC 
TACATGGTGC CGCTGCTAGT TTCGGTCGTG ACTGCAATCT GGGTTTTGGC CCTGGCGTCA 
GTGTTCCTGT GGTGCATCCG TCATCACCGT AAACAAAGCT CCAGCGCCAC CGCCATCAAC 
CCTACCAGCC CCTTCTCCAC ACCTGAAGAA AACACAGCGA ACAACGCCCG CGAGCATCTC 
AACCAGATCA AGAACCACAT TGAGAAAAAT GCCTCAAACG GCAGCCTGCC AGGAAAAGAG 
TTGCACTGTG ACGACAAAAA CACAGTCAAC GCCAAAATCA GGACGCAATT CCCTGAATCT 
GACGCCAGTA GACGACTACA AAAAACACGG TTTCCACATC AGCCAGCTTA TATGCTGGTG 
GACCGAGATG ATAGGTTGAG CTCCAATGGC ACAGACATAA AGAAACACCC CCAATGGACA 
AATAAACGCG ACAATAGAGA CCTTGAGTCG CAGCACCGGG TGCCAGATTC ACAGCACAGA 
GACTCGCAAC ACAGCTTGCA GAAAATGGAG TATATCGTAT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

jag1a ( XM_005168204.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005168261.1 Danio rerio jagged 1a (jag1a), transcript variant X1, mRNA.

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CloneID	ODa06193
Clone ID Related Accession (Same CDS sequence)	XM_005168204.4 , XM_005168204.3
Accession Version	XM_005168204.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3762bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1466611200000
Organism	Danio rerio(Zebrafish)
Product	protein jagged-1a isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007112.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005168204.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATTCTCA GACCGAGCTC AACTTTTGCG GCGCTGTCCG CTCACGTGCT GCTGAGGTGC 
TTGTGGATGC GGGTATGTGA GGCTTCGGGG CATTTCGAGA TGCAGGTTTT GTCCATGCAG 
AATGTGAACG GGGAGCTGCA GAACGGCGCG TGCTGTGACG GTGCGCGTGA CCCGGCGGAG 
CGCTCCTGCG CGGCGGACCA GTGTGATACG TTTTTCCGCG TATGTTTGAA GGAGTATCAG 
TCGCGGGTGT CTTCGGGAGG CCCTTGCAGC TACGGAAGCG GATCTACTCC TGTAATTGGT 
GGGAATACTT TTTCTGTCAA GCCCCTTGAC CAAACAAATG ACAAAACCCG GATCGTGCTG 
CCCTTCAGCT TCGCGTGGCC GCGGTCGTAT ACGCTGATTG TGGAGGCGTT GGACTTCAAT 
AATGACTCAA GCACTGGCAG CATAAATGGA CAAGTGATCG AGAAAGCGGT GCAGTCGGGC 
ATGATCAACC CAAACCGTCA ATGGCAGGTC CTGAAGCACA ACGGCCCCGT GGCTCAGTTC 
CAGTACCAAA TTCGGGTGAC CTGCGACGAG CACTACTTTG GCTTTGGCTG TAATAAGTTC 
TGTCGGCCCA GAGACGACTT TTTCGGCCAC TATACCTGCG ATCACAACGG AAACAAGACC 
TGTTTGGAAG GATGGGCCGG CCCAGAATGC AACACTGCTA TCTGTAAGCA GGGCTGCAGC 
ATCGAGCACG GCTCCTGTAA AGTGCCTGGA AACTGCAGGT GTCTGTACGG GTGGCAGGGT 
GAATACTGTG ATCAGTGTAT TCCTCATCCC GGATGTGTCC ATGGAACATG CATCGAACCC 
TGGCAGTGCC TGTGTGACAC CAACTGGGGA GGACAGCTCT GTGACAAAGA CTTAAACACC 
TGTGGCACAC TTCAGCCGTG TCTCAATGGT GGCACCTGCA GTAACACGGG ACCAGACAAA 
TACCACTGCG CCTGTCCTGA GGGCTACTCC GGACAGAACT GCGAGAGAGC TGATAACGCC 
TGTCTATCGG AGCCCTGTCT GAACGGAGGC CTCTGTGTGG AGTCCAGTCT GGGCTTTGAG 
TGTCAGTGTG CGGCCGGCTG GACGGGACCC TCCTGCAACA TCAATGAAGA CGACTGCAGT 
CCAAACCCGT GTAATCACAG CGGTGTGTGT GTGGACCTGG TGGACGGGTT CAAATGCATC 
TGCCCCGTGC AGTGGACTGG AAAGACCTGT CTCATCGATG CTAATGAGTG TGAGGAAAGC 
CCGTGCGTCA ATGCCCACTC CTGCCGCAAT CTGATTGGTG GATACTTCTG CGAGTGCCTG 
CCTGGCTGGA CGGGACAGAA TTGCGACATC AATGTCAACG ACTGCCACGG CCAGTGTCTG 
AACGGAGGTT TGTGTAAGGA TCTGGTGAAT GGATATCGGT GTGTGTGCGC AGCAGGGTTT 
GCAGGCGATC GCTGCGAGCG GGACGTGGAT GAGTGCGCCA GTCGCCCCTG TCTGAATGGC 
GGACGCTGCC AGGACACACT CAATGGATTC CAGTGTTTGT GCCCGCCTGG TTTCTCCGGT 
GCCACCTGCC AGCTGGATTT GGACTACTGT GAGAGCGGGC CGTGTCAGAA CGGGGCTCAG 
TGCTTCAGTC TGGCCTCAGA TTATTACTGT AAATGTCCAG AAGATTACGA AGGCAAGAAC 
TGCTCTCAGC TCAAAGACCA CTGCCTCATC ACACCCTGCC AAGTGATTGA CAGCTGTACG 
GTGGCGGTGG TGTCAAACAG CACCCCAGGC GGTCTGAGGC TAATCTCTTC CAGTGTGTGC 
GGTCCACACG GCCGCTGCCG TAGCCACAGC CATGCCGGAG GACACTTCAG CTGCGACTGT 
CAAGACGGCT TCACCGGGAC CTACTGCCAC GAGAACATCA ATGACTGTGA AAGCAGCCCC 
TGTCTCAGCG GAGGCACTTG CATTGACAAA ATCAATGCAT ATCAGTGTAT CTGTGCTGAC 
GGATGGGAAG GACCAAACTG CGAGACCAAC ATTGACGACT GCCGCACGAA CCCATGTCGA 
GATCGAGGAG TGTGTCGTGA CCTGGTCAAT GACTTTTACT GCGAGTGTGA GAACGGCTGG 
AAGGGAAAGA CCTGTCACTC ACGCGAGAGT CAATGCGATG AAGACACATG CAACAACGGA 
GGAACATGTT CAGATGAAGG CGACTCGTTC AAGTGCTTGT GTTCACCCGG ATGGGAAGGA 
GCAACGTGTA ATATAGCCAA GAACAGCAGC TGCCTGCCGA ACCCGTGTGA GAACGGAGCC 
ACGTGTGTGG TGACAGGCGA CGGCTTCACC TGCGTCTGCA AAGAAGGCTG GGAAGGGCCC 
ACCTGCAGCC AGAATTCCAA TGATTGCAAC CCACAACCAT GTTATAACAG TGGGACATGT 
GTGGATGGGG ATAACTGGTA CCGCTGTGAG TGCGCCAGTG GCTTTGCAGG ACCAGACTGC 
CGCATCAACA TCAATGAGTG CCAGTCGTCA CCCTGTGCGT TCGGCTCTAC CTGTGTGGAT 
GAAATTAATG GCTACCGCTG CTTGTGTCCA CCAGGCAGAA CCGGACCCAG ATGTCAGGAA 
GTCACAGGAC GCCCGTGTGT GATTGGCGGT CGGATTGCTG TTGATGGAGC AAAGTGGGCT 
GAAGACTGCA ATACTTGCTA CTGCCACAAA GGCATTGTCA CATGTACTAA GCTGTTCTGT 
GGCCCTAAAG CATGCCGTAT GCTTGGCTCG GGTCGAGGGG ACTGTCCTAC GGGTCAGATG 
TGTGTGCCGG TCCGCGATGA GCAGTGTTTC GTCAAGCCCT GTTCCTCCCA GGGCGAGTGT 
TTCGTCAAGC CCTGTTCCTC CCAGGGCGAG TGCTGGTCTG CTCACCGGCC GACCGTACGC 
ACACACTGCC AACCTGACAG CCACTGCGCA AACGTCACCT TCACCTTCAA CAAGGACACC 
ATGCCACAGG GGGTGACGGT GGAGCAGGTT TGTCGTGAAC TCAGGCATCT TTATGTGACT 
AAAAATGTTA CATCAGAGTT TTCCATCTCT GTGAGCTGTG AACTCTCTTC TGCCGCCAGT 
AACGAGATCC ATGTGGCCAT TCATGTGACT GAGAACGGCA TTCATGGCCG GGTTCCTGTT 
AAGGAAATTA CAGACAACAT CATTGATCTG GTGAGCAAAC ACAGTGCTAA CAGCTCCGTC 
ATCGGCTCCA TCGCTGAGGT TCGAGTGCAG CGCAAACAAC CGCAAAACCC CAACGTAGAC 
TACATGGTGC CGCTGCTAGT TTCGGTCGTG ACTGCAATCT GGGTTTTGGC CCTGGCGTCA 
GTGTTCCTGT GGTGCATCCG TCATCACCGT AAACAAAGCT CCAGCGCCAC CGCCATCAAC 
CCTACCAGCC CCTTCTCCAC ACCTGAAGAA AACACAGCGA ACAACGCCCG CGAGCATCTC 
AACCAGATCA AGAACCACAT TGAGAAAAAT GCCTCAAACG GCAGCCTGCC AGGAAAAGAG 
TTGCACTGTG ACGACAAAAA CACAGTCAAC GCCAAAATCA GGACGCAATT CCCTGAATCT 
GACGCCAGTA GACGACTACA AAAAACACGG TTTCCACATC AGCCAGCTTA TATGCTGGTG 
GACCGAGATG ATAGGTTGAG CTCCAATGGC ACAGACATAA AGAAACACCC CCAATGGACA 
AATAAACGCG ACAATAGAGA CCTTGAGTCG CAGCACCGGG TGCCAGATTC ACAGCACAGA 
GACTCGCAAC ACAGCTTGCA GAAAATGGAG TATATCGTAT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005168261.1
CDS	236..3997
Translation	MILRPSSTFAALSAHVLLRCLWMRVCEASGHFEMQVLSMQNVNGELQNGACCDGARDPAERSCAADQCDTFFRVCLKEYQSRVSSGGPCSYGSGSTPVIGGNTFSVKPLDQTNDKTRIVLPFSFAWPRSYTLIVEALDFNNDSSTGSINGQVIEKAVQSGMINPNRQWQVLKHNGPVAQFQYQIRVTCDEHYFGFGCNKFCRPRDDFFGHYTCDHNGNKTCLEGWAGPECNTAICKQGCSIEHGSCKVPGNCRCLYGWQGEYCDQCIPHPGCVHGTCIEPWQCLCDTNWGGQLCDKDLNTCGTLQPCLNGGTCSNTGPDKYHCACPEGYSGQNCERADNACLSEPCLNGGLCVESSLGFECQCAAGWTGPSCNINEDDCSPNPCNHSGVCVDLVDGFKCICPVQWTGKTCLIDANECEESPCVNAHSCRNLIGGYFCECLPGWTGQNCDINVNDCHGQCLNGGLCKDLVNGYRCVCAAGFAGDRCERDVDECASRPCLNGGRCQDTLNGFQCLCPPGFSGATCQLDLDYCESGPCQNGAQCFSLASDYYCKCPEDYEGKNCSQLKDHCLITPCQVIDSCTVAVVSNSTPGGLRLISSSVCGPHGRCRSHSHAGGHFSCDCQDGFTGTYCHENINDCESSPCLSGGTCIDKINAYQCICADGWEGPNCETNIDDCRTNPCRDRGVCRDLVNDFYCECENGWKGKTCHSRESQCDEDTCNNGGTCSDEGDSFKCLCSPGWEGATCNIAKNSSCLPNPCENGATCVVTGDGFTCVCKEGWEGPTCSQNSNDCNPQPCYNSGTCVDGDNWYRCECASGFAGPDCRININECQSSPCAFGSTCVDEINGYRCLCPPGRTGPRCQEVTGRPCVIGGRIAVDGAKWAEDCNTCYCHKGIVTCTKLFCGPKACRMLGSGRGDCPTGQMCVPVRDEQCFVKPCSSQGECFVKPCSSQGECWSAHRPTVRTHCQPDSHCANVTFTFNKDTMPQGVTVEQVCRELRHLYVTKNVTSEFSISVSCELSSAASNEIHVAIHVTENGIHGRVPVKEITDNIIDLVSKHSANSSVIGSIAEVRVQRKQPQNPNVDYMVPLLVSVVTAIWVLALASVFLWCIRHHRKQSSSATAINPTSPFSTPEENTANNAREHLNQIKNHIEKNASNGSLPGKELHCDDKNTVNAKIRTQFPESDASRRLQKTRFPHQPAYMLVDRDDRLSSNGTDIKKHPQWTNKRDNRDLESQHRVPDSQHRDSQHSLQKMEYIV

Target ORF information:

RefSeq Version	XM_005168204.3
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio jagged 1a (jag1a), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005168204.3

ORF Insert Sequence:

ATGATTCTCA GACCGAGCTC AACTTTTGCG GCGCTGTCCG CTCACGTGCT GCTGAGGTGC 
TTGTGGATGC GGGTATGTGA GGCTTCGGGG CATTTCGAGA TGCAGGTTTT GTCCATGCAG 
AATGTGAACG GGGAGCTGCA GAACGGCGCG TGCTGTGACG GTGCGCGTGA CCCGGCGGAG 
CGCTCCTGCG CGGCGGACCA GTGTGATACG TTTTTCCGCG TATGTTTGAA GGAGTATCAG 
TCGCGGGTGT CTTCGGGAGG CCCTTGCAGC TACGGAAGCG GATCTACTCC TGTAATTGGT 
GGGAATACTT TTTCTGTCAA GCCCCTTGAC CAAACAAATG ACAAAACCCG GATCGTGCTG 
CCCTTCAGCT TCGCGTGGCC GCGGTCGTAT ACGCTGATTG TGGAGGCGTT GGACTTCAAT 
AATGACTCAA GCACTGGCAG CATAAATGGA CAAGTGATCG AGAAAGCGGT GCAGTCGGGC 
ATGATCAACC CAAACCGTCA ATGGCAGGTC CTGAAGCACA ACGGCCCCGT GGCTCAGTTC 
CAGTACCAAA TTCGGGTGAC CTGCGACGAG CACTACTTTG GCTTTGGCTG TAATAAGTTC 
TGTCGGCCCA GAGACGACTT TTTCGGCCAC TATACCTGCG ATCACAACGG AAACAAGACC 
TGTTTGGAAG GATGGGCCGG CCCAGAATGC AACACTGCTA TCTGTAAGCA GGGCTGCAGC 
ATCGAGCACG GCTCCTGTAA AGTGCCTGGA AACTGCAGGT GTCTGTACGG GTGGCAGGGT 
GAATACTGTG ATCAGTGTAT TCCTCATCCC GGATGTGTCC ATGGAACATG CATCGAACCC 
TGGCAGTGCC TGTGTGACAC CAACTGGGGA GGACAGCTCT GTGACAAAGA CTTAAACACC 
TGTGGCACAC TTCAGCCGTG TCTCAATGGT GGCACCTGCA GTAACACGGG ACCAGACAAA 
TACCACTGCG CCTGTCCTGA GGGCTACTCC GGACAGAACT GCGAGAGAGC TGATAACGCC 
TGTCTATCGG AGCCCTGTCT GAACGGAGGC CTCTGTGTGG AGTCCAGTCT GGGCTTTGAG 
TGTCAGTGTG CGGCCGGCTG GACGGGACCC TCCTGCAACA TCAATGAAGA CGACTGCAGT 
CCAAACCCGT GTAATCACAG CGGTGTGTGT GTGGACCTGG TGGACGGGTT CAAATGCATC 
TGCCCCGTGC AGTGGACTGG AAAGACCTGT CTCATCGATG CTAATGAGTG TGAGGAAAGC 
CCGTGCGTCA ATGCCCACTC CTGCCGCAAT CTGATTGGTG GATACTTCTG CGAGTGCCTG 
CCTGGCTGGA CGGGACAGAA TTGCGACATC AATGTCAACG ACTGCCACGG CCAGTGTCTG 
AACGGAGGTT TGTGTAAGGA TCTGGTGAAT GGATATCGGT GTGTGTGCGC AGCAGGGTTT 
GCAGGCGATC GCTGCGAGCG GGACGTGGAT GAGTGCGCCA GTCGCCCCTG TCTGAATGGC 
GGACGCTGCC AGGACACACT CAATGGATTC CAGTGTTTGT GCCCGCCTGG TTTCTCCGGT 
GCCACCTGCC AGCTGGATTT GGACTACTGT GAGAGCGGGC CGTGTCAGAA CGGGGCTCAG 
TGCTTCAGTC TGGCCTCAGA TTATTACTGT AAATGTCCAG AAGATTACGA AGGCAAGAAC 
TGCTCTCAGC TCAAAGACCA CTGCCTCATC ACACCCTGCC AAGTGATTGA CAGCTGTACG 
GTGGCGGTGG TGTCAAACAG CACCCCAGGC GGTCTGAGGC TAATCTCTTC CAGTGTGTGC 
GGTCCACACG GCCGCTGCCG TAGCCACAGC CATGCCGGAG GACACTTCAG CTGCGACTGT 
CAAGACGGCT TCACCGGGAC CTACTGCCAC GAGAACATCA ATGACTGTGA AAGCAGCCCC 
TGTCTCAGCG GAGGCACTTG CATTGACAAA ATCAATGCAT ATCAGTGTAT CTGTGCTGAC 
GGATGGGAAG GACCAAACTG CGAGACCAAC ATTGACGACT GCCGCACGAA CCCATGTCGA 
GATCGAGGAG TGTGTCGTGA CCTGGTCAAT GACTTTTACT GCGAGTGTGA GAACGGCTGG 
AAGGGAAAGA CCTGTCACTC ACGCGAGAGT CAATGCGATG AAGACACATG CAACAACGGA 
GGAACATGTT CAGATGAAGG CGACTCGTTC AAGTGCTTGT GTTCACCCGG ATGGGAAGGA 
GCAACGTGTA ATATAGCCAA GAACAGCAGC TGCCTGCCGA ACCCGTGTGA GAACGGAGCC 
ACGTGTGTGG TGACAGGCGA CGGCTTCACC TGCGTCTGCA AAGAAGGCTG GGAAGGGCCC 
ACCTGCAGCC AGAATTCCAA TGATTGCAAC CCACAACCAT GTTATAACAG TGGGACATGT 
GTGGATGGGG ATAACTGGTA CCGCTGTGAG TGCGCCAGTG GCTTTGCAGG ACCAGACTGC 
CGCATCAACA TCAATGAGTG CCAGTCGTCA CCCTGTGCGT TCGGCTCTAC CTGTGTGGAT 
GAAATTAATG GCTACCGCTG CTTGTGTCCA CCAGGCAGAA CCGGACCCAG ATGTCAGGAA 
GTCACAGGAC GCCCGTGTGT GATTGGCGGT CGGATTGCTG TTGATGGAGC AAAGTGGGCT 
GAAGACTGCA ATACTTGCTA CTGCCACAAA GGCATTGTCA CATGTACTAA GCTGTTCTGT 
GGCCCTAAAG CATGCCGTAT GCTTGGCTCG GGTCGAGGGG ACTGTCCTAC GGGTCAGATG 
TGTGTGCCGG TCCGCGATGA GCAGTGTTTC GTCAAGCCCT GTTCCTCCCA GGGCGAGTGT 
TTCGTCAAGC CCTGTTCCTC CCAGGGCGAG TGCTGGTCTG CTCACCGGCC GACCGTACGC 
ACACACTGCC AACCTGACAG CCACTGCGCA AACGTCACCT TCACCTTCAA CAAGGACACC 
ATGCCACAGG GGGTGACGGT GGAGCAGGTT TGTCGTGAAC TCAGGCATCT TTATGTGACT 
AAAAATGTTA CATCAGAGTT TTCCATCTCT GTGAGCTGTG AACTCTCTTC TGCCGCCAGT 
AACGAGATCC ATGTGGCCAT TCATGTGACT GAGAACGGCA TTCATGGCCG GGTTCCTGTT 
AAGGAAATTA CAGACAACAT CATTGATCTG GTGAGCAAAC ACAGTGCTAA CAGCTCCGTC 
ATCGGCTCCA TCGCTGAGGT TCGAGTGCAG CGCAAACAAC CGCAAAACCC CAACGTAGAC 
TACATGGTGC CGCTGCTAGT TTCGGTCGTG ACTGCAATCT GGGTTTTGGC CCTGGCGTCA 
GTGTTCCTGT GGTGCATCCG TCATCACCGT AAACAAAGCT CCAGCGCCAC CGCCATCAAC 
CCTACCAGCC CCTTCTCCAC ACCTGAAGAA AACACAGCGA ACAACGCCCG CGAGCATCTC 
AACCAGATCA AGAACCACAT TGAGAAAAAT GCCTCAAACG GCAGCCTGCC AGGAAAAGAG 
TTGCACTGTG ACGACAAAAA CACAGTCAAC GCCAAAATCA GGACGCAATT CCCTGAATCT 
GACGCCAGTA GACGACTACA AAAAACACGG TTTCCACATC AGCCAGCTTA TATGCTGGTG 
GACCGAGATG ATAGGTTGAG CTCCAATGGC ACAGACATAA AGAAACACCC CCAATGGACA 
AATAAACGCG ACAATAGAGA CCTTGAGTCG CAGCACCGGG TGCCAGATTC ACAGCACAGA 
GACTCGCAAC ACAGCTTGCA GAAAATGGAG TATATCGTAT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

jag1a ( XM_005168205.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005168262.1 Danio rerio jagged 1a (jag1a), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ODa06194
Clone ID Related Accession (Same CDS sequence)	XM_005168205.3 , XM_005168205.4
Accession Version	XM_005168205.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3759bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1466611200000
Organism	Danio rerio(Zebrafish)
Product	protein jagged-1a isoform X2
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007112.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005168205.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATTCTCA GACCGAGCTC AACTTTTGCG GCGCTGTCCG CTCACGTGCT GCTGAGGTGC 
TTGTGGATGC GGGTATGTGA GGCTTCGGGG CATTTCGAGA TGCAGGTTTT GTCCATGCAG 
AATGTGAACG GGGAGCTGCA GAACGGCGCG TGCTGTGACG GTGCGCGTGA CCCGGCGGAG 
CGCTCCTGCG CGGCGGACCA GTGTGATACG TTTTTCCGCG TATGTTTGAA GGAGTATCAG 
TCGCGGGTGT CTTCGGGAGG CCCTTGCAGC TACGGAAGCG GATCTACTCC TGTAATTGGT 
GGGAATACTT TTTCTGTCAA GCCCCTTGAC CAAACAAATG ACAAAACCCG GATCGTGCTG 
CCCTTCAGCT TCGCGTGGCC GCGGTCGTAT ACGCTGATTG TGGAGGCGTT GGACTTCAAT 
AATGACTCAA GCACTGGCAG CATAAATGGA CAAGTGATCG AGAAAGCGGT GCAGTCGGGC 
ATGATCAACC CAAACCGTCA ATGGCAGGTC CTGAAGCACA ACGGCCCCGT GGCTCAGTTC 
CAGTACCAAA TTCGGGTGAC CTGCGACGAG CACTACTTTG GCTTTGGCTG TAATAAGTTC 
TGTCGGCCCA GAGACGACTT TTTCGGCCAC TATACCTGCG ATCACAACGG AAACAAGACC 
TGTTTGGAAG GATGGGCCGG CCCAGAATGC AACACTGCTA TCTGTAAGCA GGGCTGCAGC 
ATCGAGCACG GCTCCTGTAA AGTGCCTGGA AACTGCAGGT GTCTGTACGG GTGGCAGGGT 
GAATACTGTG ATCAGTGTAT TCCTCATCCC GGATGTGTCC ATGGAACATG CATCGAACCC 
TGGCAGTGCC TGTGTGACAC CAACTGGGGA GGACAGCTCT GTGACAAAGA CTTAAACACC 
TGTGGCACAC TTCAGCCGTG TCTCAATGGT GGCACCTGCA GTAACACGGG ACCAGACAAA 
TACCACTGCG CCTGTCCTGA GGGCTACTCC GGACAGAACT GCGAGAGAGC TGATAACGCC 
TGTCTATCGG AGCCCTGTCT GAACGGAGGC CTCTGTGTGG AGTCCAGTCT GGGCTTTGAG 
TGTCAGTGTG CGGCCGGCTG GACGGGACCC TCCTGCAACA TCAATGAAGA CGACTGCAGT 
CCAAACCCGT GTAATCACAG CGGTGTGTGT GTGGACCTGG TGGACGGGTT CAAATGCATC 
TGCCCCGTGC AGTGGACTGG AAAGACCTGT CTCATCGATG CTAATGAGTG TGAGGAAAGC 
CCGTGCGTCA ATGCCCACTC CTGCCGCAAT CTGATTGGTG GATACTTCTG CGAGTGCCTG 
CCTGGCTGGA CGGGACAGAA TTGCGACATC AATGTCAACG ACTGCCACGG CCAGTGTCTG 
AACGGAGGTT TGTGTAAGGA TCTGGTGAAT GGATATCGGT GTGTGTGCGC AGCAGGGTTT 
GCAGGCGATC GCTGCGAGCG GGACGTGGAT GAGTGCGCCA GTCGCCCCTG TCTGAATGGC 
GGACGCTGCC AGGACACACT CAATGGATTC CAGTGTTTGT GCCCGCCTGG TTTCTCCGGT 
GCCACCTGCC AGCTGGATTT GGACTACTGT GAGAGCGGGC CGTGTCAGAA CGGGGCTCAG 
TGCTTCAGTC TGGCCTCAGA TTATTACTGT AAATGTCCAG AAGATTACGA AGGCAAGAAC 
TGCTCTCAGC TCAAAGACCA CTGCCTCATC ACACCCTGCC AAGTGATTGA CAGCTGTACG 
GTGGCGGTGG TGTCAAACAG CACCCCAGGC GGTCTGAGGC TAATCTCTTC CAGTGTGTGC 
GGTCCACACG GCCGCTGCCG TAGCCACAGC CATGCCGGAG GACACTTCAG CTGCGACTGT 
CAAGACGGCT TCACCGGGAC CTACTGCCAC GAGAACATCA ATGACTGTGA AAGCAGCCCC 
TGTCTCAGCG GAGGCACTTG CATTGACAAA ATCAATGCAT ATCAGTGTAT CTGTGCTGAC 
GGATGGGAAG GACCAAACTG CGAGACCAAC ATTGACGACT GCCGCACGAA CCCATGTCGA 
GATCGAGGAG TGTGTCGTGA CCTGGTCAAT GACTTTTACT GCGAGTGTGA GAACGGCTGG 
AAGGGAAAGA CCTGTCACTC ACGCGAGAGT CAATGCGATG AAGACACATG CAACAACGGA 
GGAACATGTT CAGATGAAGG CGACTCGTTC AAGTGCTTGT GTTCACCCGG ATGGGAAGGA 
GCAACGTGTA ATATAGCCAA GAACAGCAGC TGCCTGCCGA ACCCGTGTGA GAACGGAGCC 
ACGTGTGTGG TGACAGGCGA CGGCTTCACC TGCGTCTGCA AAGAAGGCTG GGAAGGGCCC 
ACCTGCAGCC AGAATTCCAA TGATTGCAAC CCACAACCAT GTTATAACAG TGGGACATGT 
GTGGATGGGG ATAACTGGTA CCGCTGTGAG TGCGCCAGTG GCTTTGCAGG ACCAGACTGC 
CGCATCAACA TCAATGAGTG CCAGTCGTCA CCCTGTGCGT TCGGCTCTAC CTGTGTGGAT 
GAAATTAATG GCTACCGCTG CTTGTGTCCA CCAGGCAGAA CCGGACCCAG ATGTCAGGAA 
GTCACAGGAC GCCCGTGTGT GATTGGCGGT CGGATTGCTG TTGATGGAGC AAAGTGGGCT 
GAAGACTGCA ATACTTGCTA CTGCCACAAA GGCATTGTCA CATGTACTAA GCTGTTCTGT 
GGCCCTAAAG CATGCCGTAT GCTTGGCTCG GGTCGAGGGG ACTGTCCTAC GGGTCAGATG 
TGTGTGCCGG TCCGCGATGA GCAGTGTTTC GTCAAGCCCT GTTCCTCCCA GGGCGAGTGT 
TTCGTCAAGC CCTGTTCCTC CCAGGGCGAG TGCTGGTCTG CTCACCGGCC GACCGTACGC 
ACACACTGCC AACCTGACAG CCACTGCGCA AACGTCACCT TCACCTTCAA CAAGGACACC 
ATGCCACAGG GGGTGACGGT GGAGCAGGTT TGTCGTGAAC TCAGGCATCT TTATGTGACT 
AAAAATGTTA CATCAGAGTT TTCCATCTCT GTGAGCTGTG AACTCTCTTC TGCCGCCAGT 
AACGAGATCC ATGTGGCCAT TCATGTGACT GAGAACGGCA TTCATGGCCG GGTTCCTGTT 
AAGGAAATTA CAGACAACAT CATTGATCTG GTGAGCAAAC ACAGTGCTAA CAGCTCCGTC 
ATCGGCTCCA TCGCTGAGGT TCGAGTGCAG CGCAAACAAC CGCAAAACCC CAACGACTAC 
ATGGTGCCGC TGCTAGTTTC GGTCGTGACT GCAATCTGGG TTTTGGCCCT GGCGTCAGTG 
TTCCTGTGGT GCATCCGTCA TCACCGTAAA CAAAGCTCCA GCGCCACCGC CATCAACCCT 
ACCAGCCCCT TCTCCACACC TGAAGAAAAC ACAGCGAACA ACGCCCGCGA GCATCTCAAC 
CAGATCAAGA ACCACATTGA GAAAAATGCC TCAAACGGCA GCCTGCCAGG AAAAGAGTTG 
CACTGTGACG ACAAAAACAC AGTCAACGCC AAAATCAGGA CGCAATTCCC TGAATCTGAC 
GCCAGTAGAC GACTACAAAA AACACGGTTT CCACATCAGC CAGCTTATAT GCTGGTGGAC 
CGAGATGATA GGTTGAGCTC CAATGGCACA GACATAAAGA AACACCCCCA ATGGACAAAT 
AAACGCGACA ATAGAGACCT TGAGTCGCAG CACCGGGTGC CAGATTCACA GCACAGAGAC 
TCGCAACACA GCTTGCAGAA AATGGAGTAT ATCGTATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005168262.1
CDS	236..3994
Translation	MILRPSSTFAALSAHVLLRCLWMRVCEASGHFEMQVLSMQNVNGELQNGACCDGARDPAERSCAADQCDTFFRVCLKEYQSRVSSGGPCSYGSGSTPVIGGNTFSVKPLDQTNDKTRIVLPFSFAWPRSYTLIVEALDFNNDSSTGSINGQVIEKAVQSGMINPNRQWQVLKHNGPVAQFQYQIRVTCDEHYFGFGCNKFCRPRDDFFGHYTCDHNGNKTCLEGWAGPECNTAICKQGCSIEHGSCKVPGNCRCLYGWQGEYCDQCIPHPGCVHGTCIEPWQCLCDTNWGGQLCDKDLNTCGTLQPCLNGGTCSNTGPDKYHCACPEGYSGQNCERADNACLSEPCLNGGLCVESSLGFECQCAAGWTGPSCNINEDDCSPNPCNHSGVCVDLVDGFKCICPVQWTGKTCLIDANECEESPCVNAHSCRNLIGGYFCECLPGWTGQNCDINVNDCHGQCLNGGLCKDLVNGYRCVCAAGFAGDRCERDVDECASRPCLNGGRCQDTLNGFQCLCPPGFSGATCQLDLDYCESGPCQNGAQCFSLASDYYCKCPEDYEGKNCSQLKDHCLITPCQVIDSCTVAVVSNSTPGGLRLISSSVCGPHGRCRSHSHAGGHFSCDCQDGFTGTYCHENINDCESSPCLSGGTCIDKINAYQCICADGWEGPNCETNIDDCRTNPCRDRGVCRDLVNDFYCECENGWKGKTCHSRESQCDEDTCNNGGTCSDEGDSFKCLCSPGWEGATCNIAKNSSCLPNPCENGATCVVTGDGFTCVCKEGWEGPTCSQNSNDCNPQPCYNSGTCVDGDNWYRCECASGFAGPDCRININECQSSPCAFGSTCVDEINGYRCLCPPGRTGPRCQEVTGRPCVIGGRIAVDGAKWAEDCNTCYCHKGIVTCTKLFCGPKACRMLGSGRGDCPTGQMCVPVRDEQCFVKPCSSQGECFVKPCSSQGECWSAHRPTVRTHCQPDSHCANVTFTFNKDTMPQGVTVEQVCRELRHLYVTKNVTSEFSISVSCELSSAASNEIHVAIHVTENGIHGRVPVKEITDNIIDLVSKHSANSSVIGSIAEVRVQRKQPQNPNDYMVPLLVSVVTAIWVLALASVFLWCIRHHRKQSSSATAINPTSPFSTPEENTANNAREHLNQIKNHIEKNASNGSLPGKELHCDDKNTVNAKIRTQFPESDASRRLQKTRFPHQPAYMLVDRDDRLSSNGTDIKKHPQWTNKRDNRDLESQHRVPDSQHRDSQHSLQKMEYIV

Target ORF information:

RefSeq Version	XM_005168205.3
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio jagged 1a (jag1a), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005168205.3

ORF Insert Sequence:

ATGATTCTCA GACCGAGCTC AACTTTTGCG GCGCTGTCCG CTCACGTGCT GCTGAGGTGC 
TTGTGGATGC GGGTATGTGA GGCTTCGGGG CATTTCGAGA TGCAGGTTTT GTCCATGCAG 
AATGTGAACG GGGAGCTGCA GAACGGCGCG TGCTGTGACG GTGCGCGTGA CCCGGCGGAG 
CGCTCCTGCG CGGCGGACCA GTGTGATACG TTTTTCCGCG TATGTTTGAA GGAGTATCAG 
TCGCGGGTGT CTTCGGGAGG CCCTTGCAGC TACGGAAGCG GATCTACTCC TGTAATTGGT 
GGGAATACTT TTTCTGTCAA GCCCCTTGAC CAAACAAATG ACAAAACCCG GATCGTGCTG 
CCCTTCAGCT TCGCGTGGCC GCGGTCGTAT ACGCTGATTG TGGAGGCGTT GGACTTCAAT 
AATGACTCAA GCACTGGCAG CATAAATGGA CAAGTGATCG AGAAAGCGGT GCAGTCGGGC 
ATGATCAACC CAAACCGTCA ATGGCAGGTC CTGAAGCACA ACGGCCCCGT GGCTCAGTTC 
CAGTACCAAA TTCGGGTGAC CTGCGACGAG CACTACTTTG GCTTTGGCTG TAATAAGTTC 
TGTCGGCCCA GAGACGACTT TTTCGGCCAC TATACCTGCG ATCACAACGG AAACAAGACC 
TGTTTGGAAG GATGGGCCGG CCCAGAATGC AACACTGCTA TCTGTAAGCA GGGCTGCAGC 
ATCGAGCACG GCTCCTGTAA AGTGCCTGGA AACTGCAGGT GTCTGTACGG GTGGCAGGGT 
GAATACTGTG ATCAGTGTAT TCCTCATCCC GGATGTGTCC ATGGAACATG CATCGAACCC 
TGGCAGTGCC TGTGTGACAC CAACTGGGGA GGACAGCTCT GTGACAAAGA CTTAAACACC 
TGTGGCACAC TTCAGCCGTG TCTCAATGGT GGCACCTGCA GTAACACGGG ACCAGACAAA 
TACCACTGCG CCTGTCCTGA GGGCTACTCC GGACAGAACT GCGAGAGAGC TGATAACGCC 
TGTCTATCGG AGCCCTGTCT GAACGGAGGC CTCTGTGTGG AGTCCAGTCT GGGCTTTGAG 
TGTCAGTGTG CGGCCGGCTG GACGGGACCC TCCTGCAACA TCAATGAAGA CGACTGCAGT 
CCAAACCCGT GTAATCACAG CGGTGTGTGT GTGGACCTGG TGGACGGGTT CAAATGCATC 
TGCCCCGTGC AGTGGACTGG AAAGACCTGT CTCATCGATG CTAATGAGTG TGAGGAAAGC 
CCGTGCGTCA ATGCCCACTC CTGCCGCAAT CTGATTGGTG GATACTTCTG CGAGTGCCTG 
CCTGGCTGGA CGGGACAGAA TTGCGACATC AATGTCAACG ACTGCCACGG CCAGTGTCTG 
AACGGAGGTT TGTGTAAGGA TCTGGTGAAT GGATATCGGT GTGTGTGCGC AGCAGGGTTT 
GCAGGCGATC GCTGCGAGCG GGACGTGGAT GAGTGCGCCA GTCGCCCCTG TCTGAATGGC 
GGACGCTGCC AGGACACACT CAATGGATTC CAGTGTTTGT GCCCGCCTGG TTTCTCCGGT 
GCCACCTGCC AGCTGGATTT GGACTACTGT GAGAGCGGGC CGTGTCAGAA CGGGGCTCAG 
TGCTTCAGTC TGGCCTCAGA TTATTACTGT AAATGTCCAG AAGATTACGA AGGCAAGAAC 
TGCTCTCAGC TCAAAGACCA CTGCCTCATC ACACCCTGCC AAGTGATTGA CAGCTGTACG 
GTGGCGGTGG TGTCAAACAG CACCCCAGGC GGTCTGAGGC TAATCTCTTC CAGTGTGTGC 
GGTCCACACG GCCGCTGCCG TAGCCACAGC CATGCCGGAG GACACTTCAG CTGCGACTGT 
CAAGACGGCT TCACCGGGAC CTACTGCCAC GAGAACATCA ATGACTGTGA AAGCAGCCCC 
TGTCTCAGCG GAGGCACTTG CATTGACAAA ATCAATGCAT ATCAGTGTAT CTGTGCTGAC 
GGATGGGAAG GACCAAACTG CGAGACCAAC ATTGACGACT GCCGCACGAA CCCATGTCGA 
GATCGAGGAG TGTGTCGTGA CCTGGTCAAT GACTTTTACT GCGAGTGTGA GAACGGCTGG 
AAGGGAAAGA CCTGTCACTC ACGCGAGAGT CAATGCGATG AAGACACATG CAACAACGGA 
GGAACATGTT CAGATGAAGG CGACTCGTTC AAGTGCTTGT GTTCACCCGG ATGGGAAGGA 
GCAACGTGTA ATATAGCCAA GAACAGCAGC TGCCTGCCGA ACCCGTGTGA GAACGGAGCC 
ACGTGTGTGG TGACAGGCGA CGGCTTCACC TGCGTCTGCA AAGAAGGCTG GGAAGGGCCC 
ACCTGCAGCC AGAATTCCAA TGATTGCAAC CCACAACCAT GTTATAACAG TGGGACATGT 
GTGGATGGGG ATAACTGGTA CCGCTGTGAG TGCGCCAGTG GCTTTGCAGG ACCAGACTGC 
CGCATCAACA TCAATGAGTG CCAGTCGTCA CCCTGTGCGT TCGGCTCTAC CTGTGTGGAT 
GAAATTAATG GCTACCGCTG CTTGTGTCCA CCAGGCAGAA CCGGACCCAG ATGTCAGGAA 
GTCACAGGAC GCCCGTGTGT GATTGGCGGT CGGATTGCTG TTGATGGAGC AAAGTGGGCT 
GAAGACTGCA ATACTTGCTA CTGCCACAAA GGCATTGTCA CATGTACTAA GCTGTTCTGT 
GGCCCTAAAG CATGCCGTAT GCTTGGCTCG GGTCGAGGGG ACTGTCCTAC GGGTCAGATG 
TGTGTGCCGG TCCGCGATGA GCAGTGTTTC GTCAAGCCCT GTTCCTCCCA GGGCGAGTGT 
TTCGTCAAGC CCTGTTCCTC CCAGGGCGAG TGCTGGTCTG CTCACCGGCC GACCGTACGC 
ACACACTGCC AACCTGACAG CCACTGCGCA AACGTCACCT TCACCTTCAA CAAGGACACC 
ATGCCACAGG GGGTGACGGT GGAGCAGGTT TGTCGTGAAC TCAGGCATCT TTATGTGACT 
AAAAATGTTA CATCAGAGTT TTCCATCTCT GTGAGCTGTG AACTCTCTTC TGCCGCCAGT 
AACGAGATCC ATGTGGCCAT TCATGTGACT GAGAACGGCA TTCATGGCCG GGTTCCTGTT 
AAGGAAATTA CAGACAACAT CATTGATCTG GTGAGCAAAC ACAGTGCTAA CAGCTCCGTC 
ATCGGCTCCA TCGCTGAGGT TCGAGTGCAG CGCAAACAAC CGCAAAACCC CAACGACTAC 
ATGGTGCCGC TGCTAGTTTC GGTCGTGACT GCAATCTGGG TTTTGGCCCT GGCGTCAGTG 
TTCCTGTGGT GCATCCGTCA TCACCGTAAA CAAAGCTCCA GCGCCACCGC CATCAACCCT 
ACCAGCCCCT TCTCCACACC TGAAGAAAAC ACAGCGAACA ACGCCCGCGA GCATCTCAAC 
CAGATCAAGA ACCACATTGA GAAAAATGCC TCAAACGGCA GCCTGCCAGG AAAAGAGTTG 
CACTGTGACG ACAAAAACAC AGTCAACGCC AAAATCAGGA CGCAATTCCC TGAATCTGAC 
GCCAGTAGAC GACTACAAAA AACACGGTTT CCACATCAGC CAGCTTATAT GCTGGTGGAC 
CGAGATGATA GGTTGAGCTC CAATGGCACA GACATAAAGA AACACCCCCA ATGGACAAAT 
AAACGCGACA ATAGAGACCT TGAGTCGCAG CACCGGGTGC CAGATTCACA GCACAGAGAC 
TCGCAACACA GCTTGCAGAA AATGGAGTAT ATCGTATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

jag1a ( XM_005168205.4 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005168262.1 Danio rerio jagged 1a (jag1a), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ODa06194
Clone ID Related Accession (Same CDS sequence)	XM_005168205.3 , XM_005168205.4
Accession Version	XM_005168205.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3759bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1497369600000
Organism	Danio rerio(zebrafish)
Product	protein jagged-1a isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007112.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_005168205.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATTCTCA GACCGAGCTC AACTTTTGCG GCGCTGTCCG CTCACGTGCT GCTGAGGTGC 
TTGTGGATGC GGGTATGTGA GGCTTCGGGG CATTTCGAGA TGCAGGTTTT GTCCATGCAG 
AATGTGAACG GGGAGCTGCA GAACGGCGCG TGCTGTGACG GTGCGCGTGA CCCGGCGGAG 
CGCTCCTGCG CGGCGGACCA GTGTGATACG TTTTTCCGCG TATGTTTGAA GGAGTATCAG 
TCGCGGGTGT CTTCGGGAGG CCCTTGCAGC TACGGAAGCG GATCTACTCC TGTAATTGGT 
GGGAATACTT TTTCTGTCAA GCCCCTTGAC CAAACAAATG ACAAAACCCG GATCGTGCTG 
CCCTTCAGCT TCGCGTGGCC GCGGTCGTAT ACGCTGATTG TGGAGGCGTT GGACTTCAAT 
AATGACTCAA GCACTGGCAG CATAAATGGA CAAGTGATCG AGAAAGCGGT GCAGTCGGGC 
ATGATCAACC CAAACCGTCA ATGGCAGGTC CTGAAGCACA ACGGCCCCGT GGCTCAGTTC 
CAGTACCAAA TTCGGGTGAC CTGCGACGAG CACTACTTTG GCTTTGGCTG TAATAAGTTC 
TGTCGGCCCA GAGACGACTT TTTCGGCCAC TATACCTGCG ATCACAACGG AAACAAGACC 
TGTTTGGAAG GATGGGCCGG CCCAGAATGC AACACTGCTA TCTGTAAGCA GGGCTGCAGC 
ATCGAGCACG GCTCCTGTAA AGTGCCTGGA AACTGCAGGT GTCTGTACGG GTGGCAGGGT 
GAATACTGTG ATCAGTGTAT TCCTCATCCC GGATGTGTCC ATGGAACATG CATCGAACCC 
TGGCAGTGCC TGTGTGACAC CAACTGGGGA GGACAGCTCT GTGACAAAGA CTTAAACACC 
TGTGGCACAC TTCAGCCGTG TCTCAATGGT GGCACCTGCA GTAACACGGG ACCAGACAAA 
TACCACTGCG CCTGTCCTGA GGGCTACTCC GGACAGAACT GCGAGAGAGC TGATAACGCC 
TGTCTATCGG AGCCCTGTCT GAACGGAGGC CTCTGTGTGG AGTCCAGTCT GGGCTTTGAG 
TGTCAGTGTG CGGCCGGCTG GACGGGACCC TCCTGCAACA TCAATGAAGA CGACTGCAGT 
CCAAACCCGT GTAATCACAG CGGTGTGTGT GTGGACCTGG TGGACGGGTT CAAATGCATC 
TGCCCCGTGC AGTGGACTGG AAAGACCTGT CTCATCGATG CTAATGAGTG TGAGGAAAGC 
CCGTGCGTCA ATGCCCACTC CTGCCGCAAT CTGATTGGTG GATACTTCTG CGAGTGCCTG 
CCTGGCTGGA CGGGACAGAA TTGCGACATC AATGTCAACG ACTGCCACGG CCAGTGTCTG 
AACGGAGGTT TGTGTAAGGA TCTGGTGAAT GGATATCGGT GTGTGTGCGC AGCAGGGTTT 
GCAGGCGATC GCTGCGAGCG GGACGTGGAT GAGTGCGCCA GTCGCCCCTG TCTGAATGGC 
GGACGCTGCC AGGACACACT CAATGGATTC CAGTGTTTGT GCCCGCCTGG TTTCTCCGGT 
GCCACCTGCC AGCTGGATTT GGACTACTGT GAGAGCGGGC CGTGTCAGAA CGGGGCTCAG 
TGCTTCAGTC TGGCCTCAGA TTATTACTGT AAATGTCCAG AAGATTACGA AGGCAAGAAC 
TGCTCTCAGC TCAAAGACCA CTGCCTCATC ACACCCTGCC AAGTGATTGA CAGCTGTACG 
GTGGCGGTGG TGTCAAACAG CACCCCAGGC GGTCTGAGGC TAATCTCTTC CAGTGTGTGC 
GGTCCACACG GCCGCTGCCG TAGCCACAGC CATGCCGGAG GACACTTCAG CTGCGACTGT 
CAAGACGGCT TCACCGGGAC CTACTGCCAC GAGAACATCA ATGACTGTGA AAGCAGCCCC 
TGTCTCAGCG GAGGCACTTG CATTGACAAA ATCAATGCAT ATCAGTGTAT CTGTGCTGAC 
GGATGGGAAG GACCAAACTG CGAGACCAAC ATTGACGACT GCCGCACGAA CCCATGTCGA 
GATCGAGGAG TGTGTCGTGA CCTGGTCAAT GACTTTTACT GCGAGTGTGA GAACGGCTGG 
AAGGGAAAGA CCTGTCACTC ACGCGAGAGT CAATGCGATG AAGACACATG CAACAACGGA 
GGAACATGTT CAGATGAAGG CGACTCGTTC AAGTGCTTGT GTTCACCCGG ATGGGAAGGA 
GCAACGTGTA ATATAGCCAA GAACAGCAGC TGCCTGCCGA ACCCGTGTGA GAACGGAGCC 
ACGTGTGTGG TGACAGGCGA CGGCTTCACC TGCGTCTGCA AAGAAGGCTG GGAAGGGCCC 
ACCTGCAGCC AGAATTCCAA TGATTGCAAC CCACAACCAT GTTATAACAG TGGGACATGT 
GTGGATGGGG ATAACTGGTA CCGCTGTGAG TGCGCCAGTG GCTTTGCAGG ACCAGACTGC 
CGCATCAACA TCAATGAGTG CCAGTCGTCA CCCTGTGCGT TCGGCTCTAC CTGTGTGGAT 
GAAATTAATG GCTACCGCTG CTTGTGTCCA CCAGGCAGAA CCGGACCCAG ATGTCAGGAA 
GTCACAGGAC GCCCGTGTGT GATTGGCGGT CGGATTGCTG TTGATGGAGC AAAGTGGGCT 
GAAGACTGCA ATACTTGCTA CTGCCACAAA GGCATTGTCA CATGTACTAA GCTGTTCTGT 
GGCCCTAAAG CATGCCGTAT GCTTGGCTCG GGTCGAGGGG ACTGTCCTAC GGGTCAGATG 
TGTGTGCCGG TCCGCGATGA GCAGTGTTTC GTCAAGCCCT GTTCCTCCCA GGGCGAGTGT 
TTCGTCAAGC CCTGTTCCTC CCAGGGCGAG TGCTGGTCTG CTCACCGGCC GACCGTACGC 
ACACACTGCC AACCTGACAG CCACTGCGCA AACGTCACCT TCACCTTCAA CAAGGACACC 
ATGCCACAGG GGGTGACGGT GGAGCAGGTT TGTCGTGAAC TCAGGCATCT TTATGTGACT 
AAAAATGTTA CATCAGAGTT TTCCATCTCT GTGAGCTGTG AACTCTCTTC TGCCGCCAGT 
AACGAGATCC ATGTGGCCAT TCATGTGACT GAGAACGGCA TTCATGGCCG GGTTCCTGTT 
AAGGAAATTA CAGACAACAT CATTGATCTG GTGAGCAAAC ACAGTGCTAA CAGCTCCGTC 
ATCGGCTCCA TCGCTGAGGT TCGAGTGCAG CGCAAACAAC CGCAAAACCC CAACGACTAC 
ATGGTGCCGC TGCTAGTTTC GGTCGTGACT GCAATCTGGG TTTTGGCCCT GGCGTCAGTG 
TTCCTGTGGT GCATCCGTCA TCACCGTAAA CAAAGCTCCA GCGCCACCGC CATCAACCCT 
ACCAGCCCCT TCTCCACACC TGAAGAAAAC ACAGCGAACA ACGCCCGCGA GCATCTCAAC 
CAGATCAAGA ACCACATTGA GAAAAATGCC TCAAACGGCA GCCTGCCAGG AAAAGAGTTG 
CACTGTGACG ACAAAAACAC AGTCAACGCC AAAATCAGGA CGCAATTCCC TGAATCTGAC 
GCCAGTAGAC GACTACAAAA AACACGGTTT CCACATCAGC CAGCTTATAT GCTGGTGGAC 
CGAGATGATA GGTTGAGCTC CAATGGCACA GACATAAAGA AACACCCCCA ATGGACAAAT 
AAACGCGACA ATAGAGACCT TGAGTCGCAG CACCGGGTGC CAGATTCACA GCACAGAGAC 
TCGCAACACA GCTTGCAGAA AATGGAGTAT ATCGTATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005168262.1
CDS	251..4009
Translation	MILRPSSTFAALSAHVLLRCLWMRVCEASGHFEMQVLSMQNVNGELQNGACCDGARDPAERSCAADQCDTFFRVCLKEYQSRVSSGGPCSYGSGSTPVIGGNTFSVKPLDQTNDKTRIVLPFSFAWPRSYTLIVEALDFNNDSSTGSINGQVIEKAVQSGMINPNRQWQVLKHNGPVAQFQYQIRVTCDEHYFGFGCNKFCRPRDDFFGHYTCDHNGNKTCLEGWAGPECNTAICKQGCSIEHGSCKVPGNCRCLYGWQGEYCDQCIPHPGCVHGTCIEPWQCLCDTNWGGQLCDKDLNTCGTLQPCLNGGTCSNTGPDKYHCACPEGYSGQNCERADNACLSEPCLNGGLCVESSLGFECQCAAGWTGPSCNINEDDCSPNPCNHSGVCVDLVDGFKCICPVQWTGKTCLIDANECEESPCVNAHSCRNLIGGYFCECLPGWTGQNCDINVNDCHGQCLNGGLCKDLVNGYRCVCAAGFAGDRCERDVDECASRPCLNGGRCQDTLNGFQCLCPPGFSGATCQLDLDYCESGPCQNGAQCFSLASDYYCKCPEDYEGKNCSQLKDHCLITPCQVIDSCTVAVVSNSTPGGLRLISSSVCGPHGRCRSHSHAGGHFSCDCQDGFTGTYCHENINDCESSPCLSGGTCIDKINAYQCICADGWEGPNCETNIDDCRTNPCRDRGVCRDLVNDFYCECENGWKGKTCHSRESQCDEDTCNNGGTCSDEGDSFKCLCSPGWEGATCNIAKNSSCLPNPCENGATCVVTGDGFTCVCKEGWEGPTCSQNSNDCNPQPCYNSGTCVDGDNWYRCECASGFAGPDCRININECQSSPCAFGSTCVDEINGYRCLCPPGRTGPRCQEVTGRPCVIGGRIAVDGAKWAEDCNTCYCHKGIVTCTKLFCGPKACRMLGSGRGDCPTGQMCVPVRDEQCFVKPCSSQGECFVKPCSSQGECWSAHRPTVRTHCQPDSHCANVTFTFNKDTMPQGVTVEQVCRELRHLYVTKNVTSEFSISVSCELSSAASNEIHVAIHVTENGIHGRVPVKEITDNIIDLVSKHSANSSVIGSIAEVRVQRKQPQNPNDYMVPLLVSVVTAIWVLALASVFLWCIRHHRKQSSSATAINPTSPFSTPEENTANNAREHLNQIKNHIEKNASNGSLPGKELHCDDKNTVNAKIRTQFPESDASRRLQKTRFPHQPAYMLVDRDDRLSSNGTDIKKHPQWTNKRDNRDLESQHRVPDSQHRDSQHSLQKMEYIV

Target ORF information:

RefSeq Version	XM_005168205.4
Organism	Danio rerio(zebrafish)
Definition	Danio rerio jagged 1a (jag1a), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005168205.4

ORF Insert Sequence:

ATGATTCTCA GACCGAGCTC AACTTTTGCG GCGCTGTCCG CTCACGTGCT GCTGAGGTGC 
TTGTGGATGC GGGTATGTGA GGCTTCGGGG CATTTCGAGA TGCAGGTTTT GTCCATGCAG 
AATGTGAACG GGGAGCTGCA GAACGGCGCG TGCTGTGACG GTGCGCGTGA CCCGGCGGAG 
CGCTCCTGCG CGGCGGACCA GTGTGATACG TTTTTCCGCG TATGTTTGAA GGAGTATCAG 
TCGCGGGTGT CTTCGGGAGG CCCTTGCAGC TACGGAAGCG GATCTACTCC TGTAATTGGT 
GGGAATACTT TTTCTGTCAA GCCCCTTGAC CAAACAAATG ACAAAACCCG GATCGTGCTG 
CCCTTCAGCT TCGCGTGGCC GCGGTCGTAT ACGCTGATTG TGGAGGCGTT GGACTTCAAT 
AATGACTCAA GCACTGGCAG CATAAATGGA CAAGTGATCG AGAAAGCGGT GCAGTCGGGC 
ATGATCAACC CAAACCGTCA ATGGCAGGTC CTGAAGCACA ACGGCCCCGT GGCTCAGTTC 
CAGTACCAAA TTCGGGTGAC CTGCGACGAG CACTACTTTG GCTTTGGCTG TAATAAGTTC 
TGTCGGCCCA GAGACGACTT TTTCGGCCAC TATACCTGCG ATCACAACGG AAACAAGACC 
TGTTTGGAAG GATGGGCCGG CCCAGAATGC AACACTGCTA TCTGTAAGCA GGGCTGCAGC 
ATCGAGCACG GCTCCTGTAA AGTGCCTGGA AACTGCAGGT GTCTGTACGG GTGGCAGGGT 
GAATACTGTG ATCAGTGTAT TCCTCATCCC GGATGTGTCC ATGGAACATG CATCGAACCC 
TGGCAGTGCC TGTGTGACAC CAACTGGGGA GGACAGCTCT GTGACAAAGA CTTAAACACC 
TGTGGCACAC TTCAGCCGTG TCTCAATGGT GGCACCTGCA GTAACACGGG ACCAGACAAA 
TACCACTGCG CCTGTCCTGA GGGCTACTCC GGACAGAACT GCGAGAGAGC TGATAACGCC 
TGTCTATCGG AGCCCTGTCT GAACGGAGGC CTCTGTGTGG AGTCCAGTCT GGGCTTTGAG 
TGTCAGTGTG CGGCCGGCTG GACGGGACCC TCCTGCAACA TCAATGAAGA CGACTGCAGT 
CCAAACCCGT GTAATCACAG CGGTGTGTGT GTGGACCTGG TGGACGGGTT CAAATGCATC 
TGCCCCGTGC AGTGGACTGG AAAGACCTGT CTCATCGATG CTAATGAGTG TGAGGAAAGC 
CCGTGCGTCA ATGCCCACTC CTGCCGCAAT CTGATTGGTG GATACTTCTG CGAGTGCCTG 
CCTGGCTGGA CGGGACAGAA TTGCGACATC AATGTCAACG ACTGCCACGG CCAGTGTCTG 
AACGGAGGTT TGTGTAAGGA TCTGGTGAAT GGATATCGGT GTGTGTGCGC AGCAGGGTTT 
GCAGGCGATC GCTGCGAGCG GGACGTGGAT GAGTGCGCCA GTCGCCCCTG TCTGAATGGC 
GGACGCTGCC AGGACACACT CAATGGATTC CAGTGTTTGT GCCCGCCTGG TTTCTCCGGT 
GCCACCTGCC AGCTGGATTT GGACTACTGT GAGAGCGGGC CGTGTCAGAA CGGGGCTCAG 
TGCTTCAGTC TGGCCTCAGA TTATTACTGT AAATGTCCAG AAGATTACGA AGGCAAGAAC 
TGCTCTCAGC TCAAAGACCA CTGCCTCATC ACACCCTGCC AAGTGATTGA CAGCTGTACG 
GTGGCGGTGG TGTCAAACAG CACCCCAGGC GGTCTGAGGC TAATCTCTTC CAGTGTGTGC 
GGTCCACACG GCCGCTGCCG TAGCCACAGC CATGCCGGAG GACACTTCAG CTGCGACTGT 
CAAGACGGCT TCACCGGGAC CTACTGCCAC GAGAACATCA ATGACTGTGA AAGCAGCCCC 
TGTCTCAGCG GAGGCACTTG CATTGACAAA ATCAATGCAT ATCAGTGTAT CTGTGCTGAC 
GGATGGGAAG GACCAAACTG CGAGACCAAC ATTGACGACT GCCGCACGAA CCCATGTCGA 
GATCGAGGAG TGTGTCGTGA CCTGGTCAAT GACTTTTACT GCGAGTGTGA GAACGGCTGG 
AAGGGAAAGA CCTGTCACTC ACGCGAGAGT CAATGCGATG AAGACACATG CAACAACGGA 
GGAACATGTT CAGATGAAGG CGACTCGTTC AAGTGCTTGT GTTCACCCGG ATGGGAAGGA 
GCAACGTGTA ATATAGCCAA GAACAGCAGC TGCCTGCCGA ACCCGTGTGA GAACGGAGCC 
ACGTGTGTGG TGACAGGCGA CGGCTTCACC TGCGTCTGCA AAGAAGGCTG GGAAGGGCCC 
ACCTGCAGCC AGAATTCCAA TGATTGCAAC CCACAACCAT GTTATAACAG TGGGACATGT 
GTGGATGGGG ATAACTGGTA CCGCTGTGAG TGCGCCAGTG GCTTTGCAGG ACCAGACTGC 
CGCATCAACA TCAATGAGTG CCAGTCGTCA CCCTGTGCGT TCGGCTCTAC CTGTGTGGAT 
GAAATTAATG GCTACCGCTG CTTGTGTCCA CCAGGCAGAA CCGGACCCAG ATGTCAGGAA 
GTCACAGGAC GCCCGTGTGT GATTGGCGGT CGGATTGCTG TTGATGGAGC AAAGTGGGCT 
GAAGACTGCA ATACTTGCTA CTGCCACAAA GGCATTGTCA CATGTACTAA GCTGTTCTGT 
GGCCCTAAAG CATGCCGTAT GCTTGGCTCG GGTCGAGGGG ACTGTCCTAC GGGTCAGATG 
TGTGTGCCGG TCCGCGATGA GCAGTGTTTC GTCAAGCCCT GTTCCTCCCA GGGCGAGTGT 
TTCGTCAAGC CCTGTTCCTC CCAGGGCGAG TGCTGGTCTG CTCACCGGCC GACCGTACGC 
ACACACTGCC AACCTGACAG CCACTGCGCA AACGTCACCT TCACCTTCAA CAAGGACACC 
ATGCCACAGG GGGTGACGGT GGAGCAGGTT TGTCGTGAAC TCAGGCATCT TTATGTGACT 
AAAAATGTTA CATCAGAGTT TTCCATCTCT GTGAGCTGTG AACTCTCTTC TGCCGCCAGT 
AACGAGATCC ATGTGGCCAT TCATGTGACT GAGAACGGCA TTCATGGCCG GGTTCCTGTT 
AAGGAAATTA CAGACAACAT CATTGATCTG GTGAGCAAAC ACAGTGCTAA CAGCTCCGTC 
ATCGGCTCCA TCGCTGAGGT TCGAGTGCAG CGCAAACAAC CGCAAAACCC CAACGACTAC 
ATGGTGCCGC TGCTAGTTTC GGTCGTGACT GCAATCTGGG TTTTGGCCCT GGCGTCAGTG 
TTCCTGTGGT GCATCCGTCA TCACCGTAAA CAAAGCTCCA GCGCCACCGC CATCAACCCT 
ACCAGCCCCT TCTCCACACC TGAAGAAAAC ACAGCGAACA ACGCCCGCGA GCATCTCAAC 
CAGATCAAGA ACCACATTGA GAAAAATGCC TCAAACGGCA GCCTGCCAGG AAAAGAGTTG 
CACTGTGACG ACAAAAACAC AGTCAACGCC AAAATCAGGA CGCAATTCCC TGAATCTGAC 
GCCAGTAGAC GACTACAAAA AACACGGTTT CCACATCAGC CAGCTTATAT GCTGGTGGAC 
CGAGATGATA GGTTGAGCTC CAATGGCACA GACATAAAGA AACACCCCCA ATGGACAAAT 
AAACGCGACA ATAGAGACCT TGAGTCGCAG CACCGGGTGC CAGATTCACA GCACAGAGAC 
TCGCAACACA GCTTGCAGAA AATGGAGTAT ATCGTATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Wnt/β-catenin signaling controls intrahepatic biliary network formation in zebrafish by regulating notch activity.
Hepatology (Baltimore, Md.)67(6)2352-2366(2018 06)
So J,Khaliq M,Evason K,Ninov N,Martin BL,Stainier DYR,Shin D

Ultrafine Particle Exposure Reveals the Importance of FOXO1/Notch Activation Complex for Vascular Regeneration.
Antioxidants & redox signaling28(13)1209-1223(2018 05)
Baek KI,Packard RRS,Hsu JJ,Saffari A,Ma Z,Luu AP,Pietersen A,Yen H,Ren B,Ding Y,Sioutas C,Li R,Hsiai TK

itFISH: Enhanced Staining by Iterative Fluorescent In Situ Hybridization.
Zebrafish14(6)578-580(2017 12)
Row RH,Martin BL

Automated Segmentation of Light-Sheet Fluorescent Imaging to Characterize Experimental Doxorubicin-Induced Cardiac Injury and Repair.
Scientific reports7(1)8603(2017 08)
Packard RRS,Baek KI,Beebe T,Jen N,Ding Y,Shi F,Fei P,Kang BJ,Chen PH,Gau J,Chen M,Tang JY,Shih YH,Ding Y,Li D,Xu X,Hsiai TK

Zebrafish Caudal Haematopoietic Embryonic Stromal Tissue (CHEST) Cells Support Haematopoiesis.
Scientific reports744644(2017 03)
Wolf A,Aggio J,Campbell C,Wright F,Marquez G,Traver D,Stachura DL

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jag1a cDNA ORF clone, Danio rerio(zebrafish)

Gene

Clones

gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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