ddx58 cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	ddx58
Entrez Gene ID	100333797
Full Name	DEAD (Asp-Glu-Ala-Asp) box polypeptide 58
Synonyms	RIG-I,RIG-Ia,RIG-Ib,RIG-Ic,RIG-Id
General protein information	Preferred Names DEAD (Asp-Glu-Ala-Asp) box polypeptide 58 Names probable ATP-dependent RNA helicase DDX58 RIG-1 retinoic acid-inducible protein Ia retinoic acid-inducible protein Ib retinoic acid-inducible protein Ic retinoic acid-inducible protein Id rig1
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001306095.1	NP_001293024.1	probable ATP-dependent RNA helicase DDX58

Pathways from BioSystems

Homologies

Rattus norvegicus (Norway rat)	Ddx58	NP_001100115.1
Danio rerio (zebrafish)	LOC100333797	XP_002666571.3

Gene Ontology
Bibliography

Related articles in PubMed

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Liu L, Hu Y, Lu J, Wang G
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Lai Y, Liang M, Hu, Zeng Z, Lin H, Yi G, Li M, Liu Z
Fish & shellfish immunology86474-479(2019 Mar)

Neddylation Facilitates the Antiviral Response in Zebrafish.
Yu G, Liu X, Tang J, Xu C, Ouyang G, Xiao W
Frontiers in immunology101432(2019)

Synthesis and antiviral activity of a new coumarin derivative against spring viraemia of carp virus.
Shen YF, Liu L, Feng CZ, Hu Y, Chen C, Wang GX, Zhu B
Fish & shellfish immunology8157-66(2018 Oct)

TANK-Binding Kinase 1 (TBK1) Isoforms Negatively Regulate Type I Interferon Induction by Inhibiting TBK1-IRF3 Interaction and IRF3 Phosphorylation.
Hu YW, Zhang J, Wu XM, Cao L, Nie P, Chang MX
Frontiers in immunology984(2018)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following ddx58 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ddx58 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ODa53152	NM_001306095.1 Latest version!	Danio rerio DEAD (Asp-Glu-Ala-Asp) box polypeptide 58 (ddx58), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

ddx58 ( NM_001306095.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_001293024.1 Danio rerio DEAD (Asp-Glu-Ala-Asp) box polypeptide 58 (ddx58), mRNA.

Price & Availability↑

CloneID	ODa53152
Clone ID Related Accession (Same CDS sequence)	NM_001306095.1
Accession Version	NM_001306095.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2814bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1573747200000
Organism	Danio rerio(zebrafish)
Product	probable ATP-dependent RNA helicase DDX58
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from KM281808.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: KM281808.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA2168446, SAMEA2168447 [ECO:0000350] ##Evidence-Data-END##

ATGTACGAGC TGGAGAAGGA GAATCTGCGG CGCTTGAGCT CTTACATCAC GGCCATTATA 
CGACCGTCAC TAATAAAAAC CTTCATGACG ACATACCTGA AGAAAGAAAC AGTGGAGCGG 
ATTATGTCCA CGGAGCAGAG CTCTCCGTCT GCAGCCGCTC AGATGCTGCT GGACTGCATG 
TGTGATCTGG AGGAGGACGG CTGGTTTCAG GCCTTTCTGG ACTTCCTACT CACTGCTGAG 
TACTCCGGCC TGCACGATGC CATTAAAAAC TGGAACTTCC TCGAGTACGA GGAGCTGCAT 
GAGCACCGGA AGCTTCTGGA GAGAATCGAA CCCTCAATCA CCAAACACTT GAAGCCCAGA 
GAGCTGCTCA TGCACATGAA CTGCTGCCTG AACCAGAGGG AGTCTGAGGA GATTCAAGCG 
GTGGAAGCGC AGCGTGGACC GATCGCCGCC AGTGAGAAGC TTGTGGACTG TCTCCTGCGC 
TCAGATAAAT CAAACTGGTT TAAGGTGCTG AAGATGGCTC TGGACACCTG CGAATTATAT 
GAGGCTCTCG ACCTGCTGGA GACAGATGGA GCAGGAAAGT CAGTGCAGGA CTCAGACTGC 
ATGATGGCTG AATCCTCCAC TGTGTGCTTT GAGTACCGAG AGGAGATGGA GACAGAAAAC 
ATGGCGGCGC CGGAGAGCTG CAGCCACATG AGCATCACTG AGACACCTGC TGGAGTTACA 
GGTGACTCTG GGCGTTCAGG TAAGCGTAAG CTGAGGGAAT ATCAGAGAGA GCTGATGACT 
GCAGCCGTCA ACGGACAGAA CACCATCATC TGCGCTCCTA CAGGTTGTGG GAAGACTATC 
GTGGCGCTGG GCATCTGTGA AGAGCATTTG AAGCTGCTGC GCGGACGAGC AAAGATCGCC 
TTCCTGGCCA CTAAAGTGGA CGTTTATGAG CAGCAGTACA AACTCTTCCA GGAGCACTTC 
AGCAGCAAAG ACCCCAACAT CAGGGTGACG GGGATGTGTG GAGATATGGC TCTCTCAGTG 
CGGATGCTGA TCGAGACTCA TGATATTGTG GTCCTGACGG CTCAGATTCT GGTGAACGCC 
CTGCAGAGCG GAGAGATGGA GAACCTGCAG CTGCTGACGC TCATTCTGCT GGACGAGTGC 
CACAACACCA CCGGCAAACA CCCCTACAAC AATATCATGA CCCGCTACCT GGACAGCAAG 
CTCACCGGCA ACGGCAGCCC ACTGCCACAG ATTGTGGGCC TGACAGCATC GGTTGGCATC 
GGTTCCTTCA AAAACCAGAT GGAGGCGGAG AACAATGTTA CACAGCTGTG TGCGAACATG 
GACACCAGAG TCATTACCAC TGTTAGCAAA CACCTGGACG AGCTCAGGTC ATACGTGCAC 
ACGCCGGAGA AAGAATTTTT CCATGTGTTT CAGTCGCCGT CAGATCCCTT CATCAGAATC 
ATCCAGAACA TCATGAGTAA CATTGAGCAG ATGGCGAAGA AAACCTACAA CATCGAGAGT 
CTGTCGAACA TCTCGAATCG TGAGTATGGC TCTCAGAAGT ACGAGCAGTG GATCGTGTCG 
GTGCAGAAGA GCTGCAGAGT TTTGCAGATG CAGAACGCAG ACGGGGAGCG ACGCATCTGC 
AGAGAGCTCT GCAACTACAC TGAACACCTG CGGAAATATA ACGACGCTCT GATCATTAAT 
GAAGACGCTC GCACTATAGA CGCTCTGGAT TATCTGGACG TGTTTTTCCA GCAGGTCCGA 
AACGCCGGAT TCCAGACCAC AGAGAGGAAA CTCACGGCGC TTTACGACAC CCAGCGTCCG 
CAGCTGCAGT GTTTGGCCAC TGAGGGGCAG CAGAACCCCA AACTGGAGGA GCTGCGCTTC 
ATCCTGGAGG AGGAATACCA CAACAATCAG GAGACGCGCA CCGTCATGTT CGTCCGCACA 
CGAGCCCTCG CTGACGCCAT GAAGAAATGG ATTGACGACA CTGATACTCT GAAGTTTCTG 
AAGCCCGGAG TTCTGATTGG AAAAGGCCGG AAATCCAACT TTACTGGTTC AGGAATGACC 
CCCAGTAATC AGAGGGGTGT TCTGGACACG TTTAAGAGCT CAGATCAGAG TAAGATGCTC 
ATCGCCACAT CAGTCGCCGA CGAGGGCATC GATATCCCAC AATGCAACCT GGTTCTGATG 
TACGAGTATG TGGGGAACGT GGTGAAGATG GTGCAGGTTC GAGGTCGTGG GCGTGCGCAG 
GGCAGTCGGT GTTTCCTGAT CTCCAGCAGT GCCGAACGCA TCGAGAAGGA GAAGATGAAC 
ATGATGAAGG AGAGACTGGT GGAGGCGGCC ATTGTGGCTC TGCAGAGTCG CCCTGCGGAC 
CTCAGTTTCA AGGTGGACAT ATTACAGAAG GAAGATAAAG CGCGGAGAGA TTTCCTCAGC 
GCCAGTCCTG ATAAACCCAA AACACAGGAC AGCTTCAAGC TCCTCTGCGC AAAGTGCAAG 
ATGTTTGCGT GTCAGAGCGA CGACCTGCGA GTCGTGCAGG AGTCTCATCA CATAGTTGTG 
GACCGCTCCA TATTCTCCCG CTGCTCCAGA GTTCCTCACA GAAAGCCCAT CAACTTCTGT 
GGCTTCAGTA AGAAGGAGAA GATGCTGTGT GTGGAGTGCA AACACGACTG GGGGCTCGTC 
GTCTCATACC TCACCATACA GGATCTGCCG CTGCTGAAGA TCGAGAGTTT CGTGGTGCAG 
AATTTGGTCA CTGGAGAGCA GCATTACTTC AGAAAGTGGT GCAAGGTGAC GTTCGCCATA 
CGAGAGTTTG ACATGACGGA GATCACACCT GAGACATGGG CGCTGGTCAA CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001293024.1
CDS	101..2914
Translation	MYELEKENLRRLSSYITAIIRPSLIKTFMTTYLKKETVERIMSTEQSSPSAAAQMLLDCMCDLEEDGWFQAFLDFLLTAEYSGLHDAIKNWNFLEYEELHEHRKLLERIEPSITKHLKPRELLMHMNCCLNQRESEEIQAVEAQRGPIAASEKLVDCLLRSDKSNWFKVLKMALDTCELYEALDLLETDGAGKSVQDSDCMMAESSTVCFEYREEMETENMAAPESCSHMSITETPAGVTGDSGRSGKRKLREYQRELMTAAVNGQNTIICAPTGCGKTIVALGICEEHLKLLRGRAKIAFLATKVDVYEQQYKLFQEHFSSKDPNIRVTGMCGDMALSVRMLIETHDIVVLTAQILVNALQSGEMENLQLLTLILLDECHNTTGKHPYNNIMTRYLDSKLTGNGSPLPQIVGLTASVGIGSFKNQMEAENNVTQLCANMDTRVITTVSKHLDELRSYVHTPEKEFFHVFQSPSDPFIRIIQNIMSNIEQMAKKTYNIESLSNISNREYGSQKYEQWIVSVQKSCRVLQMQNADGERRICRELCNYTEHLRKYNDALIINEDARTIDALDYLDVFFQQVRNAGFQTTERKLTALYDTQRPQLQCLATEGQQNPKLEELRFILEEEYHNNQETRTVMFVRTRALADAMKKWIDDTDTLKFLKPGVLIGKGRKSNFTGSGMTPSNQRGVLDTFKSSDQSKMLIATSVADEGIDIPQCNLVLMYEYVGNVVKMVQVRGRGRAQGSRCFLISSSAERIEKEKMNMMKERLVEAAIVALQSRPADLSFKVDILQKEDKARRDFLSASPDKPKTQDSFKLLCAKCKMFACQSDDLRVVQESHHIVVDRSIFSRCSRVPHRKPINFCGFSKKEKMLCVECKHDWGLVVSYLTIQDLPLLKIESFVVQNLVTGEQHYFRKWCKVTFAIREFDMTEITPETWALVN

Target ORF information:

RefSeq Version	NM_001306095.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio DEAD (Asp-Glu-Ala-Asp) box polypeptide 58 (ddx58), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001306095.1

ORF Insert Sequence:

ATGTACGAGC TGGAGAAGGA GAATCTGCGG CGCTTGAGCT CTTACATCAC GGCCATTATA 
CGACCGTCAC TAATAAAAAC CTTCATGACG ACATACCTGA AGAAAGAAAC AGTGGAGCGG 
ATTATGTCCA CGGAGCAGAG CTCTCCGTCT GCAGCCGCTC AGATGCTGCT GGACTGCATG 
TGTGATCTGG AGGAGGACGG CTGGTTTCAG GCCTTTCTGG ACTTCCTACT CACTGCTGAG 
TACTCCGGCC TGCACGATGC CATTAAAAAC TGGAACTTCC TCGAGTACGA GGAGCTGCAT 
GAGCACCGGA AGCTTCTGGA GAGAATCGAA CCCTCAATCA CCAAACACTT GAAGCCCAGA 
GAGCTGCTCA TGCACATGAA CTGCTGCCTG AACCAGAGGG AGTCTGAGGA GATTCAAGCG 
GTGGAAGCGC AGCGTGGACC GATCGCCGCC AGTGAGAAGC TTGTGGACTG TCTCCTGCGC 
TCAGATAAAT CAAACTGGTT TAAGGTGCTG AAGATGGCTC TGGACACCTG CGAATTATAT 
GAGGCTCTCG ACCTGCTGGA GACAGATGGA GCAGGAAAGT CAGTGCAGGA CTCAGACTGC 
ATGATGGCTG AATCCTCCAC TGTGTGCTTT GAGTACCGAG AGGAGATGGA GACAGAAAAC 
ATGGCGGCGC CGGAGAGCTG CAGCCACATG AGCATCACTG AGACACCTGC TGGAGTTACA 
GGTGACTCTG GGCGTTCAGG TAAGCGTAAG CTGAGGGAAT ATCAGAGAGA GCTGATGACT 
GCAGCCGTCA ACGGACAGAA CACCATCATC TGCGCTCCTA CAGGTTGTGG GAAGACTATC 
GTGGCGCTGG GCATCTGTGA AGAGCATTTG AAGCTGCTGC GCGGACGAGC AAAGATCGCC 
TTCCTGGCCA CTAAAGTGGA CGTTTATGAG CAGCAGTACA AACTCTTCCA GGAGCACTTC 
AGCAGCAAAG ACCCCAACAT CAGGGTGACG GGGATGTGTG GAGATATGGC TCTCTCAGTG 
CGGATGCTGA TCGAGACTCA TGATATTGTG GTCCTGACGG CTCAGATTCT GGTGAACGCC 
CTGCAGAGCG GAGAGATGGA GAACCTGCAG CTGCTGACGC TCATTCTGCT GGACGAGTGC 
CACAACACCA CCGGCAAACA CCCCTACAAC AATATCATGA CCCGCTACCT GGACAGCAAG 
CTCACCGGCA ACGGCAGCCC ACTGCCACAG ATTGTGGGCC TGACAGCATC GGTTGGCATC 
GGTTCCTTCA AAAACCAGAT GGAGGCGGAG AACAATGTTA CACAGCTGTG TGCGAACATG 
GACACCAGAG TCATTACCAC TGTTAGCAAA CACCTGGACG AGCTCAGGTC ATACGTGCAC 
ACGCCGGAGA AAGAATTTTT CCATGTGTTT CAGTCGCCGT CAGATCCCTT CATCAGAATC 
ATCCAGAACA TCATGAGTAA CATTGAGCAG ATGGCGAAGA AAACCTACAA CATCGAGAGT 
CTGTCGAACA TCTCGAATCG TGAGTATGGC TCTCAGAAGT ACGAGCAGTG GATCGTGTCG 
GTGCAGAAGA GCTGCAGAGT TTTGCAGATG CAGAACGCAG ACGGGGAGCG ACGCATCTGC 
AGAGAGCTCT GCAACTACAC TGAACACCTG CGGAAATATA ACGACGCTCT GATCATTAAT 
GAAGACGCTC GCACTATAGA CGCTCTGGAT TATCTGGACG TGTTTTTCCA GCAGGTCCGA 
AACGCCGGAT TCCAGACCAC AGAGAGGAAA CTCACGGCGC TTTACGACAC CCAGCGTCCG 
CAGCTGCAGT GTTTGGCCAC TGAGGGGCAG CAGAACCCCA AACTGGAGGA GCTGCGCTTC 
ATCCTGGAGG AGGAATACCA CAACAATCAG GAGACGCGCA CCGTCATGTT CGTCCGCACA 
CGAGCCCTCG CTGACGCCAT GAAGAAATGG ATTGACGACA CTGATACTCT GAAGTTTCTG 
AAGCCCGGAG TTCTGATTGG AAAAGGCCGG AAATCCAACT TTACTGGTTC AGGAATGACC 
CCCAGTAATC AGAGGGGTGT TCTGGACACG TTTAAGAGCT CAGATCAGAG TAAGATGCTC 
ATCGCCACAT CAGTCGCCGA CGAGGGCATC GATATCCCAC AATGCAACCT GGTTCTGATG 
TACGAGTATG TGGGGAACGT GGTGAAGATG GTGCAGGTTC GAGGTCGTGG GCGTGCGCAG 
GGCAGTCGGT GTTTCCTGAT CTCCAGCAGT GCCGAACGCA TCGAGAAGGA GAAGATGAAC 
ATGATGAAGG AGAGACTGGT GGAGGCGGCC ATTGTGGCTC TGCAGAGTCG CCCTGCGGAC 
CTCAGTTTCA AGGTGGACAT ATTACAGAAG GAAGATAAAG CGCGGAGAGA TTTCCTCAGC 
GCCAGTCCTG ATAAACCCAA AACACAGGAC AGCTTCAAGC TCCTCTGCGC AAAGTGCAAG 
ATGTTTGCGT GTCAGAGCGA CGACCTGCGA GTCGTGCAGG AGTCTCATCA CATAGTTGTG 
GACCGCTCCA TATTCTCCCG CTGCTCCAGA GTTCCTCACA GAAAGCCCAT CAACTTCTGT 
GGCTTCAGTA AGAAGGAGAA GATGCTGTGT GTGGAGTGCA AACACGACTG GGGGCTCGTC 
GTCTCATACC TCACCATACA GGATCTGCCG CTGCTGAAGA TCGAGAGTTT CGTGGTGCAG 
AATTTGGTCA CTGGAGAGCA GCATTACTTC AGAAAGTGGT GCAAGGTGAC GTTCGCCATA 
CGAGAGTTTG ACATGACGGA GATCACACCT GAGACATGGG CGCTGGTCAA CTGA