eftud2 cDNA ORF clone, Takifugu rubripes(torafugu)

Gene
Clones
gRNAs

Summary

Gene Symbol	eftud2
Entrez Gene ID	101074480
Full Name	elongation factor Tu GTP binding domain containing 2
Gene Type	protein-coding
Organism	Takifugu rubripes(torafugu)

mRNA and Protein(s)

mRNA	Protein	Name
XM_003964812.2	XP_003964861.1	116 kDa U5 small nuclear ribonucleoprotein component
XM_003964812.3	XP_003964861.1	116 kDa U5 small nuclear ribonucleoprotein component

Pathways from BioSystems
Gene Ontology

The following eftud2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the eftud2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OTo03814	XM_003964812.2 Latest version!	Takifugu rubripes elongation factor Tu GTP binding domain containing 2 (eftud2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
OTo67001	XM_003964812.3 Latest version!	Takifugu rubripes elongation factor Tu GTP binding domain containing 2 (eftud2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

eftud2 ( XM_003964812.2 ) cDNA ORF clone, Takifugu rubripes(torafugu) -> XP_003964861.1 Takifugu rubripes elongation factor Tu GTP binding domain containing 2 (eftud2), mRNA.

Price & Availability↑

CloneID	OTo03814
Clone ID Related Accession (Same CDS sequence)	XM_003964812.2
Accession Version	XM_003964812.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2916bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1426348800000
Organism	Takifugu rubripes(Fugu rubripes)
Product	116 kDa U5 small nuclear ribonucleoprotein component
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_004070961.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Mar 16, 2015 this sequence version replaced XM_003964812.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Takifugu rubripes Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAGCCG ATCTGTATGA TGAGTTTGGT AACTACATCG GTCCAGAGCT GGATTCTGAC 
GATGATGAAG ATGAACTGGA TGCAGAGGAC AGAGATGTTG ATGAGGGTGA TGAGGATGAT 
GAAGATGAAC CCGCCGATGC TGATGACGAT GTCCCAGGTA TGGAGGTCGT ACTACACGAG 
GACAAGAAAT ACTATCCTAC TGCAGAAGAG GTGTATGGAC CAGAGGTGGA AACCATTGTC 
CAAGAGGAAG ACACACAACC TCTTACAGAG CCCATTATTA AGCCAGTAAG GCACCGGCAG 
TTTACCCTGA TGGAGCAGGA GTTACCTGCC ACCGTGTATG ATATGGAATT CCTCGCAGAC 
CTGATGGACA GTTCTGAGCT GATCCGAAAT GTCACTCTGT GCGGACACCT CCATCATGGC 
AAGACCTGCT TTGTGGACTG CCTGATTGAA CAAACACATC CAGAAATCAG GAAGAGATAT 
GATGTTGATC TCCGATACAC AGACACGCTG TTCACGGAAC AAGAGAGGGG CGTTGGCATC 
AAGAGTACCC CCGTCACAAT GGTGTTGCCT GACTCCAGAG GCAAATCTTA TCTTTTCAAC 
GTTATGGATA CTCCAGGCCA CATAAACTTC TCTGATGAAG TCACATCAAG TATCCGGATC 
TCGGACGGAA TTGTCCTCTT CATAGATGCA GCAGAAGGGG TGATGTTGAA CACAGAGCGC 
CTGATTAAAC ATGCTGTCCA GGAGCGAATG GCCATCACCA TCTGCATCAA CAAGGTGGAC 
AGGCTGATTC TGGAGCTCAA ATTACCTCCC ACAGATGCTT ATTATAAACT TCGCCACATT 
GTAGACGAGG TCAACGGTTT GCTGAACACG TACTCCACAG ATGAGACCAT GGTGGTCTCT 
CCTCTGCTTG GAAATGTGTG TTTCGCCAGT CCTCAGTACA GCATCTGCTT CACCCTGGGC 
TCTTTTGCTA AAATCTACGC AGACACATAC GGAGACATCA ACTATACCGA GTTTTCCAAG 
AGGCTCTGGG GTGACATTTA CTTTAACCCT AAAACCCACA AGTTTACCAA GAAAGCTCCC 
ACCAGTAATT CCCAGCGCAG TTTTGTGGAA TTTGTCCTGG AACCTTTGTA TAAGATTCTC 
TCTCAGGTGG TGGGGGACGT GGACACGTCT CTCCCACGAG TTCTAGATGA GCTGGGGATC 
CATCTCTCTA AAGAGGAGCT CAAGCTGAAC ATCAAACCTT TGCTTAGGTT GGTGTGTAAC 
CGCTTCTTTG GAGAATTCAC CGGCTTTGTG GACATGTGCG TGCAACACAT CCCCTCACCA 
CAGAGGGGTG CTAGAACCAA GATAGAGCAT ACCTATACAG GAGGGCTGGA CTCGGACCTG 
GCAGAGGCGA TGACAGAGTG TGATCCTGAA GGTCCTTTGA TGTGCCACAC CACTAAGATG 
TACAGCACAG AGGACGGGGT TCAGTTCCAT GCATTTGGCA GGGTGCTCAG TGGGACCATC 
CAGGCAGGGC AACCTGTCAA GGTTTTAGGA GAGAACTACA CTCTGGAAGA TGAGGAGGAC 
TCACAGATCT GCACTGTTGG CCGTCTTTGG ATTTCAGTTG CCAGGTACCA AATTGAGGTA 
AACAGAGTTC CTGCTGGAAA CTGGGTCCTC ATCGAAGGTT GCGACCAGCC CATTGTCAAG 
ACCGCCACGA TCACTGAGCC CAGAGGGAAC GAGGAGGCTC AGATTTTCAG GCCATTGAAG 
TTCAACACGG CATCAGTTAT AAAAATTGCC GTGGAGCCTG TCAACCCATC AGAGCTCCCA 
AAGATGTTGG ATGGTCTGAG GAAGGTCAAC AAGAGTTATC CATCTCTCAC CACAAAGGTG 
GAAGAGTCAG GAGAGCACGT TATCTTGGGA ACAGGGGAGC TTTACTTGGA CTGTGTCATG 
CACGACCTGC GCAAGATGTA CTCTGAAATT GACATTAAAG TCGCCGACCC CGTTGTGACC 
TTTTGTGAGA CGGTTGTGGA GACGTCGTCA CTCAAGTGCT TTGCTGAAAC GCCCAACAAA 
AAGAATAAGA TCACCATGAT TGCAGAGCCC CTGGAGAAGG GACTGGCTGA GGACATTGAG 
AACGAAGTTG TGCAGATCAC ATGGAACAGG AAGAAGCTGG GGGAGTTTTT CCAGACAAAG 
TATGACTGGG ATCTGCTGGC TGCCAGGTCC ATCTGGGCCT TTGGACCAGA CACCACGGGT 
CCAAACATCC TCGTGGACGA CACGCTGCCC TCTGAGGTGG ACAAGGCGCT GCTGGGCTCG 
GTCAAAGACA GCATCGTTCA GGGCTTCCAG TGGGGCACCA GGGAGGGGCC TCTGTGCGAC 
GAGCCGATCA GGAACGTCAA GTTTAAGATC CTGGACGCCG TCATTGCTCA GGAGCCTCTC 
CACAGAGGCG GGGGCCAGGT CATACCTACA GCCAGAAGAG TGGTGTACTC CGCCTTCCTC 
ATGGCCACTC CAAGGTTGAT GGAGCCGTAT TACTTTGTGG AAGTTCAGGC TCCGGCTGAT 
TGTGTTTCTG CCGTGTACAC CGTGCTGGCT CGGAGAAGGG GTCACGTCAC CCAGGACGCG 
CCCATTCCAG GCTCTCCTCT CTACACCATC AAGGCCTTCA TCCCCGCCAT CGACTCGTTT 
GGCTTTGAGA CCGACCTCCG CACACACACA CAGGGGCAGG CCTTCGCTCT CTCTGTGTTC 
CACCACTGGC AGATCGTTCC CGGTGACCCT CTGGATAAAA GCATCGTGAT CAGGCCGCTG 
GAGCCACAAC CCGCCCCCCA CCTGGCCAGA GAGTTCATGA TCAAGACCCG GAGGCGCAAG 
GGTCTGAGCG AGGACGTCAG CATCAGCAAG TTCTTCGACG ATCCTATGTT GTTGGAGTTG 
GCCAAACAGG ACGTGGTGCT CAACTACCCC ATGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_003964861.1
CDS	158..3073
Translation	MEADLYDEFGNYIGPELDSDDDEDELDAEDRDVDEGDEDDEDEPADADDDVPGMEVVLHEDKKYYPTAEEVYGPEVETIVQEEDTQPLTEPIIKPVRHRQFTLMEQELPATVYDMEFLADLMDSSELIRNVTLCGHLHHGKTCFVDCLIEQTHPEIRKRYDVDLRYTDTLFTEQERGVGIKSTPVTMVLPDSRGKSYLFNVMDTPGHINFSDEVTSSIRISDGIVLFIDAAEGVMLNTERLIKHAVQERMAITICINKVDRLILELKLPPTDAYYKLRHIVDEVNGLLNTYSTDETMVVSPLLGNVCFASPQYSICFTLGSFAKIYADTYGDINYTEFSKRLWGDIYFNPKTHKFTKKAPTSNSQRSFVEFVLEPLYKILSQVVGDVDTSLPRVLDELGIHLSKEELKLNIKPLLRLVCNRFFGEFTGFVDMCVQHIPSPQRGARTKIEHTYTGGLDSDLAEAMTECDPEGPLMCHTTKMYSTEDGVQFHAFGRVLSGTIQAGQPVKVLGENYTLEDEEDSQICTVGRLWISVARYQIEVNRVPAGNWVLIEGCDQPIVKTATITEPRGNEEAQIFRPLKFNTASVIKIAVEPVNPSELPKMLDGLRKVNKSYPSLTTKVEESGEHVILGTGELYLDCVMHDLRKMYSEIDIKVADPVVTFCETVVETSSLKCFAETPNKKNKITMIAEPLEKGLAEDIENEVVQITWNRKKLGEFFQTKYDWDLLAARSIWAFGPDTTGPNILVDDTLPSEVDKALLGSVKDSIVQGFQWGTREGPLCDEPIRNVKFKILDAVIAQEPLHRGGGQVIPTARRVVYSAFLMATPRLMEPYYFVEVQAPADCVSAVYTVLARRRGHVTQDAPIPGSPLYTIKAFIPAIDSFGFETDLRTHTQGQAFALSVFHHWQIVPGDPLDKSIVIRPLEPQPAPHLAREFMIKTRRRKGLSEDVSISKFFDDPMLLELAKQDVVLNYPM

Target ORF information:

RefSeq Version	XM_003964812.2
Organism	Takifugu rubripes(Fugu rubripes)
Definition	Takifugu rubripes elongation factor Tu GTP binding domain containing 2 (eftud2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_003964812.2

ORF Insert Sequence:

ATGGAAGCCG ATCTGTATGA TGAGTTTGGT AACTACATCG GTCCAGAGCT GGATTCTGAC 
GATGATGAAG ATGAACTGGA TGCAGAGGAC AGAGATGTTG ATGAGGGTGA TGAGGATGAT 
GAAGATGAAC CCGCCGATGC TGATGACGAT GTCCCAGGTA TGGAGGTCGT ACTACACGAG 
GACAAGAAAT ACTATCCTAC TGCAGAAGAG GTGTATGGAC CAGAGGTGGA AACCATTGTC 
CAAGAGGAAG ACACACAACC TCTTACAGAG CCCATTATTA AGCCAGTAAG GCACCGGCAG 
TTTACCCTGA TGGAGCAGGA GTTACCTGCC ACCGTGTATG ATATGGAATT CCTCGCAGAC 
CTGATGGACA GTTCTGAGCT GATCCGAAAT GTCACTCTGT GCGGACACCT CCATCATGGC 
AAGACCTGCT TTGTGGACTG CCTGATTGAA CAAACACATC CAGAAATCAG GAAGAGATAT 
GATGTTGATC TCCGATACAC AGACACGCTG TTCACGGAAC AAGAGAGGGG CGTTGGCATC 
AAGAGTACCC CCGTCACAAT GGTGTTGCCT GACTCCAGAG GCAAATCTTA TCTTTTCAAC 
GTTATGGATA CTCCAGGCCA CATAAACTTC TCTGATGAAG TCACATCAAG TATCCGGATC 
TCGGACGGAA TTGTCCTCTT CATAGATGCA GCAGAAGGGG TGATGTTGAA CACAGAGCGC 
CTGATTAAAC ATGCTGTCCA GGAGCGAATG GCCATCACCA TCTGCATCAA CAAGGTGGAC 
AGGCTGATTC TGGAGCTCAA ATTACCTCCC ACAGATGCTT ATTATAAACT TCGCCACATT 
GTAGACGAGG TCAACGGTTT GCTGAACACG TACTCCACAG ATGAGACCAT GGTGGTCTCT 
CCTCTGCTTG GAAATGTGTG TTTCGCCAGT CCTCAGTACA GCATCTGCTT CACCCTGGGC 
TCTTTTGCTA AAATCTACGC AGACACATAC GGAGACATCA ACTATACCGA GTTTTCCAAG 
AGGCTCTGGG GTGACATTTA CTTTAACCCT AAAACCCACA AGTTTACCAA GAAAGCTCCC 
ACCAGTAATT CCCAGCGCAG TTTTGTGGAA TTTGTCCTGG AACCTTTGTA TAAGATTCTC 
TCTCAGGTGG TGGGGGACGT GGACACGTCT CTCCCACGAG TTCTAGATGA GCTGGGGATC 
CATCTCTCTA AAGAGGAGCT CAAGCTGAAC ATCAAACCTT TGCTTAGGTT GGTGTGTAAC 
CGCTTCTTTG GAGAATTCAC CGGCTTTGTG GACATGTGCG TGCAACACAT CCCCTCACCA 
CAGAGGGGTG CTAGAACCAA GATAGAGCAT ACCTATACAG GAGGGCTGGA CTCGGACCTG 
GCAGAGGCGA TGACAGAGTG TGATCCTGAA GGTCCTTTGA TGTGCCACAC CACTAAGATG 
TACAGCACAG AGGACGGGGT TCAGTTCCAT GCATTTGGCA GGGTGCTCAG TGGGACCATC 
CAGGCAGGGC AACCTGTCAA GGTTTTAGGA GAGAACTACA CTCTGGAAGA TGAGGAGGAC 
TCACAGATCT GCACTGTTGG CCGTCTTTGG ATTTCAGTTG CCAGGTACCA AATTGAGGTA 
AACAGAGTTC CTGCTGGAAA CTGGGTCCTC ATCGAAGGTT GCGACCAGCC CATTGTCAAG 
ACCGCCACGA TCACTGAGCC CAGAGGGAAC GAGGAGGCTC AGATTTTCAG GCCATTGAAG 
TTCAACACGG CATCAGTTAT AAAAATTGCC GTGGAGCCTG TCAACCCATC AGAGCTCCCA 
AAGATGTTGG ATGGTCTGAG GAAGGTCAAC AAGAGTTATC CATCTCTCAC CACAAAGGTG 
GAAGAGTCAG GAGAGCACGT TATCTTGGGA ACAGGGGAGC TTTACTTGGA CTGTGTCATG 
CACGACCTGC GCAAGATGTA CTCTGAAATT GACATTAAAG TCGCCGACCC CGTTGTGACC 
TTTTGTGAGA CGGTTGTGGA GACGTCGTCA CTCAAGTGCT TTGCTGAAAC GCCCAACAAA 
AAGAATAAGA TCACCATGAT TGCAGAGCCC CTGGAGAAGG GACTGGCTGA GGACATTGAG 
AACGAAGTTG TGCAGATCAC ATGGAACAGG AAGAAGCTGG GGGAGTTTTT CCAGACAAAG 
TATGACTGGG ATCTGCTGGC TGCCAGGTCC ATCTGGGCCT TTGGACCAGA CACCACGGGT 
CCAAACATCC TCGTGGACGA CACGCTGCCC TCTGAGGTGG ACAAGGCGCT GCTGGGCTCG 
GTCAAAGACA GCATCGTTCA GGGCTTCCAG TGGGGCACCA GGGAGGGGCC TCTGTGCGAC 
GAGCCGATCA GGAACGTCAA GTTTAAGATC CTGGACGCCG TCATTGCTCA GGAGCCTCTC 
CACAGAGGCG GGGGCCAGGT CATACCTACA GCCAGAAGAG TGGTGTACTC CGCCTTCCTC 
ATGGCCACTC CAAGGTTGAT GGAGCCGTAT TACTTTGTGG AAGTTCAGGC TCCGGCTGAT 
TGTGTTTCTG CCGTGTACAC CGTGCTGGCT CGGAGAAGGG GTCACGTCAC CCAGGACGCG 
CCCATTCCAG GCTCTCCTCT CTACACCATC AAGGCCTTCA TCCCCGCCAT CGACTCGTTT 
GGCTTTGAGA CCGACCTCCG CACACACACA CAGGGGCAGG CCTTCGCTCT CTCTGTGTTC 
CACCACTGGC AGATCGTTCC CGGTGACCCT CTGGATAAAA GCATCGTGAT CAGGCCGCTG 
GAGCCACAAC CCGCCCCCCA CCTGGCCAGA GAGTTCATGA TCAAGACCCG GAGGCGCAAG 
GGTCTGAGCG AGGACGTCAG CATCAGCAAG TTCTTCGACG ATCCTATGTT GTTGGAGTTG 
GCCAAACAGG ACGTGGTGCT CAACTACCCC ATGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

eftud2 ( XM_003964812.3 ) cDNA ORF clone, Takifugu rubripes(torafugu) -> XP_003964861.1 Takifugu rubripes elongation factor Tu GTP binding domain containing 2 (eftud2), mRNA.

Price & Availability↑

CloneID	OTo67001
Clone ID Related Accession (Same CDS sequence)	XM_003964812.3
Accession Version	XM_003964812.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2916bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1562169600000
Organism	Takifugu rubripes(torafugu)
Product	116 kDa U5 small nuclear ribonucleoprotein component
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_042289.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Jul 5, 2019 this sequence version replaced XM_003964812.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Takifugu rubripes Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAGCCG ATCTGTATGA TGAGTTTGGT AACTACATCG GTCCAGAGCT GGATTCTGAC 
GATGATGAAG ATGAACTGGA TGCAGAGGAC AGAGATGTTG ATGAGGGTGA TGAGGATGAT 
GAAGATGAAC CCGCCGATGC TGATGACGAT GTCCCAGGTA TGGAGGTCGT ACTACACGAG 
GACAAGAAAT ACTATCCTAC TGCAGAAGAG GTGTATGGAC CAGAGGTGGA AACCATTGTC 
CAAGAGGAAG ACACACAACC TCTTACAGAG CCCATTATTA AGCCAGTAAG GCACCGGCAG 
TTTACCCTGA TGGAGCAGGA GTTACCTGCC ACCGTGTATG ATATGGAATT CCTCGCAGAC 
CTGATGGACA GTTCTGAGCT GATCCGAAAT GTCACTCTGT GCGGACACCT CCATCATGGC 
AAGACCTGCT TTGTGGACTG CCTGATTGAA CAAACACATC CAGAAATCAG GAAGAGATAT 
GATGTTGATC TCCGATACAC AGACACGCTG TTCACGGAAC AAGAGAGGGG CGTTGGCATC 
AAGAGTACCC CCGTCACAAT GGTGTTGCCT GACTCCAGAG GCAAATCTTA TCTTTTCAAC 
GTTATGGATA CTCCAGGCCA CATAAACTTC TCTGATGAAG TCACATCAAG TATCCGGATC 
TCGGACGGAA TTGTCCTCTT CATAGATGCA GCAGAAGGGG TGATGTTGAA CACAGAGCGC 
CTGATTAAAC ATGCTGTCCA GGAGCGAATG GCCATCACCA TCTGCATCAA CAAGGTGGAC 
AGGCTGATTC TGGAGCTCAA ATTACCTCCC ACAGATGCTT ATTATAAACT TCGCCACATT 
GTAGACGAGG TCAACGGTTT GCTGAACACG TACTCCACAG ATGAGACCAT GGTGGTCTCT 
CCTCTGCTTG GAAATGTGTG TTTCGCCAGC CCTCAGTACA GCATCTGCTT CACCCTGGGC 
TCTTTTGCTA AAATCTACGC AGACACATAC GGAGACATCA ACTATACCGA GTTTTCCAAG 
AGGCTCTGGG GTGACATTTA CTTTAACCCT AAAACCCACA AGTTTACCAA GAAAGCTCCC 
ACCAGTAATT CCCAGCGCAG TTTTGTGGAA TTTGTCCTGG AACCTTTGTA TAAGATTCTC 
TCTCAGGTGG TGGGGGACGT GGACACGTCT CTCCCACGAG TTCTAGATGA GCTGGGGATC 
CATCTCTCTA AAGAGGAGCT CAAGCTGAAC ATCAAACCTT TGCTTAGGTT GGTGTGTAAC 
CGCTTCTTTG GAGAATTCAC CGGCTTTGTG GACATGTGCG TGCAACACAT CCCCTCACCA 
CAGAGGGGTG CTAGAACCAA GATAGAGCAT ACCTATACAG GAGGGCTGGA CTCGGACCTG 
GCAGAGGCGA TGACAGAGTG TGATCCTGAA GGTCCTTTGA TGTGCCACAC CACTAAGATG 
TACAGCACAG AGGACGGGGT TCAGTTCCAT GCATTTGGCA GGGTGCTCAG TGGGACCATC 
CAGGCAGGGC AACCTGTCAA GGTTTTAGGA GAGAACTACA CTCTGGAAGA TGAGGAGGAC 
TCACAGATCT GCACTGTTGG CCGTCTTTGG ATTTCAGTTG CCAGGTACCA AATTGAGGTA 
AACAGAGTTC CTGCTGGAAA CTGGGTCCTC ATCGAAGGTT GCGACCAGCC CATTGTCAAG 
ACCGCCACGA TCACTGAGCC CAGAGGGAAC GAGGAGGCTC AGATTTTCAG GCCATTGAAG 
TTCAACACGG CATCAGTTAT AAAAATTGCC GTGGAGCCTG TCAACCCATC AGAGCTCCCA 
AAGATGTTGG ATGGTCTGAG GAAGGTCAAC AAGAGTTATC CATCGCTCAC CACAAAGGTG 
GAAGAGTCAG GAGAGCACGT TATCTTGGGA ACAGGGGAGC TTTACTTGGA CTGTGTCATG 
CACGACCTAC GCAAGATGTA CTCTGAAATT GACATTAAAG TCGCCGACCC CGTTGTGACC 
TTTTGTGAGA CGGTTGTGGA GACGTCGTCA CTCAAGTGCT TTGCTGAAAC GCCCAACAAA 
AAGAATAAGA TCACCATGAT TGCAGAGCCC CTGGAGAAGG GACTGGCTGA GGACATTGAG 
AACGAAGTTG TGCAGATCAC ATGGAACAGG AAGAAGCTGG GGGAGTTTTT CCAGACAAAG 
TATGACTGGG ATCTGCTGGC TGCCAGGTCC ATCTGGGCCT TTGGACCAGA CACCACGGGT 
CCAAACATCC TCGTGGACGA CACGCTGCCC TCTGAGGTGG ACAAGGCGCT GCTGGGCTCG 
GTCAAAGACA GCATCGTTCA GGGCTTCCAG TGGGGCACCA GGGAGGGGCC TCTGTGCGAC 
GAGCCGATCA GGAACGTCAA GTTTAAGATC CTGGACGCCG TCATTGCTCA GGAGCCTCTC 
CACAGAGGCG GGGGCCAGGT CATACCTACA GCCAGAAGAG TGGTGTACTC CGCCTTCCTC 
ATGGCCACTC CAAGGTTGAT GGAGCCGTAT TACTTTGTGG AAGTTCAGGC TCCGGCTGAT 
TGTGTTTCTG CCGTGTACAC CGTGCTGGCT CGGAGAAGGG GTCACGTCAC CCAGGACGCG 
CCCATTCCAG GCTCTCCTCT CTACACCATC AAGGCCTTCA TCCCCGCCAT CGACTCGTTT 
GGCTTTGAGA CCGACCTCCG CACACACACA CAGGGGCAGG CCTTCGCTCT CTCTGTGTTC 
CACCACTGGC AGATCGTTCC CGGTGACCCT CTGGATAAAA GCATCGTGAT CAGGCCGCTG 
GAGCCACAAC CCGCCCCCCA CCTGGCCAGA GAGTTCATGA TCAAGACCCG GAGGCGCAAG 
GGTCTGAGCG AGGACGTCAG CATCAGCAAG TTCTTCGACG ATCCTATGTT GTTGGAGTTG 
GCCAAACAGG ACGTGGTGCT CAACTACCCC ATGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_003964861.1
CDS	177..3092
Translation	MEADLYDEFGNYIGPELDSDDDEDELDAEDRDVDEGDEDDEDEPADADDDVPGMEVVLHEDKKYYPTAEEVYGPEVETIVQEEDTQPLTEPIIKPVRHRQFTLMEQELPATVYDMEFLADLMDSSELIRNVTLCGHLHHGKTCFVDCLIEQTHPEIRKRYDVDLRYTDTLFTEQERGVGIKSTPVTMVLPDSRGKSYLFNVMDTPGHINFSDEVTSSIRISDGIVLFIDAAEGVMLNTERLIKHAVQERMAITICINKVDRLILELKLPPTDAYYKLRHIVDEVNGLLNTYSTDETMVVSPLLGNVCFASPQYSICFTLGSFAKIYADTYGDINYTEFSKRLWGDIYFNPKTHKFTKKAPTSNSQRSFVEFVLEPLYKILSQVVGDVDTSLPRVLDELGIHLSKEELKLNIKPLLRLVCNRFFGEFTGFVDMCVQHIPSPQRGARTKIEHTYTGGLDSDLAEAMTECDPEGPLMCHTTKMYSTEDGVQFHAFGRVLSGTIQAGQPVKVLGENYTLEDEEDSQICTVGRLWISVARYQIEVNRVPAGNWVLIEGCDQPIVKTATITEPRGNEEAQIFRPLKFNTASVIKIAVEPVNPSELPKMLDGLRKVNKSYPSLTTKVEESGEHVILGTGELYLDCVMHDLRKMYSEIDIKVADPVVTFCETVVETSSLKCFAETPNKKNKITMIAEPLEKGLAEDIENEVVQITWNRKKLGEFFQTKYDWDLLAARSIWAFGPDTTGPNILVDDTLPSEVDKALLGSVKDSIVQGFQWGTREGPLCDEPIRNVKFKILDAVIAQEPLHRGGGQVIPTARRVVYSAFLMATPRLMEPYYFVEVQAPADCVSAVYTVLARRRGHVTQDAPIPGSPLYTIKAFIPAIDSFGFETDLRTHTQGQAFALSVFHHWQIVPGDPLDKSIVIRPLEPQPAPHLAREFMIKTRRRKGLSEDVSISKFFDDPMLLELAKQDVVLNYPM

Target ORF information:

RefSeq Version	XM_003964812.3
Organism	Takifugu rubripes(torafugu)
Definition	Takifugu rubripes elongation factor Tu GTP binding domain containing 2 (eftud2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_003964812.3

ORF Insert Sequence:

ATGGAAGCCG ATCTGTATGA TGAGTTTGGT AACTACATCG GTCCAGAGCT GGATTCTGAC 
GATGATGAAG ATGAACTGGA TGCAGAGGAC AGAGATGTTG ATGAGGGTGA TGAGGATGAT 
GAAGATGAAC CCGCCGATGC TGATGACGAT GTCCCAGGTA TGGAGGTCGT ACTACACGAG 
GACAAGAAAT ACTATCCTAC TGCAGAAGAG GTGTATGGAC CAGAGGTGGA AACCATTGTC 
CAAGAGGAAG ACACACAACC TCTTACAGAG CCCATTATTA AGCCAGTAAG GCACCGGCAG 
TTTACCCTGA TGGAGCAGGA GTTACCTGCC ACCGTGTATG ATATGGAATT CCTCGCAGAC 
CTGATGGACA GTTCTGAGCT GATCCGAAAT GTCACTCTGT GCGGACACCT CCATCATGGC 
AAGACCTGCT TTGTGGACTG CCTGATTGAA CAAACACATC CAGAAATCAG GAAGAGATAT 
GATGTTGATC TCCGATACAC AGACACGCTG TTCACGGAAC AAGAGAGGGG CGTTGGCATC 
AAGAGTACCC CCGTCACAAT GGTGTTGCCT GACTCCAGAG GCAAATCTTA TCTTTTCAAC 
GTTATGGATA CTCCAGGCCA CATAAACTTC TCTGATGAAG TCACATCAAG TATCCGGATC 
TCGGACGGAA TTGTCCTCTT CATAGATGCA GCAGAAGGGG TGATGTTGAA CACAGAGCGC 
CTGATTAAAC ATGCTGTCCA GGAGCGAATG GCCATCACCA TCTGCATCAA CAAGGTGGAC 
AGGCTGATTC TGGAGCTCAA ATTACCTCCC ACAGATGCTT ATTATAAACT TCGCCACATT 
GTAGACGAGG TCAACGGTTT GCTGAACACG TACTCCACAG ATGAGACCAT GGTGGTCTCT 
CCTCTGCTTG GAAATGTGTG TTTCGCCAGC CCTCAGTACA GCATCTGCTT CACCCTGGGC 
TCTTTTGCTA AAATCTACGC AGACACATAC GGAGACATCA ACTATACCGA GTTTTCCAAG 
AGGCTCTGGG GTGACATTTA CTTTAACCCT AAAACCCACA AGTTTACCAA GAAAGCTCCC 
ACCAGTAATT CCCAGCGCAG TTTTGTGGAA TTTGTCCTGG AACCTTTGTA TAAGATTCTC 
TCTCAGGTGG TGGGGGACGT GGACACGTCT CTCCCACGAG TTCTAGATGA GCTGGGGATC 
CATCTCTCTA AAGAGGAGCT CAAGCTGAAC ATCAAACCTT TGCTTAGGTT GGTGTGTAAC 
CGCTTCTTTG GAGAATTCAC CGGCTTTGTG GACATGTGCG TGCAACACAT CCCCTCACCA 
CAGAGGGGTG CTAGAACCAA GATAGAGCAT ACCTATACAG GAGGGCTGGA CTCGGACCTG 
GCAGAGGCGA TGACAGAGTG TGATCCTGAA GGTCCTTTGA TGTGCCACAC CACTAAGATG 
TACAGCACAG AGGACGGGGT TCAGTTCCAT GCATTTGGCA GGGTGCTCAG TGGGACCATC 
CAGGCAGGGC AACCTGTCAA GGTTTTAGGA GAGAACTACA CTCTGGAAGA TGAGGAGGAC 
TCACAGATCT GCACTGTTGG CCGTCTTTGG ATTTCAGTTG CCAGGTACCA AATTGAGGTA 
AACAGAGTTC CTGCTGGAAA CTGGGTCCTC ATCGAAGGTT GCGACCAGCC CATTGTCAAG 
ACCGCCACGA TCACTGAGCC CAGAGGGAAC GAGGAGGCTC AGATTTTCAG GCCATTGAAG 
TTCAACACGG CATCAGTTAT AAAAATTGCC GTGGAGCCTG TCAACCCATC AGAGCTCCCA 
AAGATGTTGG ATGGTCTGAG GAAGGTCAAC AAGAGTTATC CATCGCTCAC CACAAAGGTG 
GAAGAGTCAG GAGAGCACGT TATCTTGGGA ACAGGGGAGC TTTACTTGGA CTGTGTCATG 
CACGACCTAC GCAAGATGTA CTCTGAAATT GACATTAAAG TCGCCGACCC CGTTGTGACC 
TTTTGTGAGA CGGTTGTGGA GACGTCGTCA CTCAAGTGCT TTGCTGAAAC GCCCAACAAA 
AAGAATAAGA TCACCATGAT TGCAGAGCCC CTGGAGAAGG GACTGGCTGA GGACATTGAG 
AACGAAGTTG TGCAGATCAC ATGGAACAGG AAGAAGCTGG GGGAGTTTTT CCAGACAAAG 
TATGACTGGG ATCTGCTGGC TGCCAGGTCC ATCTGGGCCT TTGGACCAGA CACCACGGGT 
CCAAACATCC TCGTGGACGA CACGCTGCCC TCTGAGGTGG ACAAGGCGCT GCTGGGCTCG 
GTCAAAGACA GCATCGTTCA GGGCTTCCAG TGGGGCACCA GGGAGGGGCC TCTGTGCGAC 
GAGCCGATCA GGAACGTCAA GTTTAAGATC CTGGACGCCG TCATTGCTCA GGAGCCTCTC 
CACAGAGGCG GGGGCCAGGT CATACCTACA GCCAGAAGAG TGGTGTACTC CGCCTTCCTC 
ATGGCCACTC CAAGGTTGAT GGAGCCGTAT TACTTTGTGG AAGTTCAGGC TCCGGCTGAT 
TGTGTTTCTG CCGTGTACAC CGTGCTGGCT CGGAGAAGGG GTCACGTCAC CCAGGACGCG 
CCCATTCCAG GCTCTCCTCT CTACACCATC AAGGCCTTCA TCCCCGCCAT CGACTCGTTT 
GGCTTTGAGA CCGACCTCCG CACACACACA CAGGGGCAGG CCTTCGCTCT CTCTGTGTTC 
CACCACTGGC AGATCGTTCC CGGTGACCCT CTGGATAAAA GCATCGTGAT CAGGCCGCTG 
GAGCCACAAC CCGCCCCCCA CCTGGCCAGA GAGTTCATGA TCAAGACCCG GAGGCGCAAG 
GGTCTGAGCG AGGACGTCAG CATCAGCAAG TTCTTCGACG ATCCTATGTT GTTGGAGTTG 
GCCAAACAGG ACGTGGTGCT CAACTACCCC ATGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

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eftud2 cDNA ORF clone, Takifugu rubripes(torafugu)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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