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processing

Before mRNA processing, a newly synthesized RNA molecule is a complementary copy of the DNA strand that served as the template for RNA synthesis. processing or maturation of the RNA molecule to its final form can involve endo- or exonucleolytic cleavage or cleavage/resealing steps (splicing), that remove specific regions of the RNA molecule before the RNA becomes biologically functional. Since a cDNA molecule is generally synthesized using an mRNA molecule as a template, the cDNA sequence corresponds to that of the mature, or fully processed RNA molecule. Regions such as the extreme 5´ terminus and initially transcribed intervening sequences are generally absent from an isolated cDNA molecule. Certain characteristic processing site sequence features, such as the junctions associated with removal of intervening sequences, may be retained in a cDNA molecule, but the biological significance of such features must be confirmed by comparison with the genomic DNA sequence.

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Service and Products

Gene Synthesis

Offer gene synthesis of 100% sequence guarantee, free codon optimization, DNA synthesis in as fast as 4 days.

ORF cDNA Clones and Custom Clones

Largest commercial ORF clones database, 70,000 next-day shipping ORF clones.

Plasmid DNA Preparation

High quality plasmids supported by fully-automated platform, 100% full insert sequence accuracy guaranteed.

Express Cloning & Subcloning

$49 and 2 day turnaround, choose from over 150 IP FREE vectors.

Mutation and Library Services

Mutagenesis Portfolio offers several mutation and library services to meet various customers’ needs.

Oligo Synthesis

Bridge the gap between chemically synthesized oligos and bological applications.

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