LOC542479 cDNA ORF clone, Zea mays

Gene
Clones
gRNAs

Summary

Gene Symbol	LOC542479
Entrez Gene ID	542479
Full Name	phosphoenolpyruvate carboxylase 7
Synonyms	GRMZM2G069542,Ppc1C,pepcZm2A
Gene Type	protein-coding
Organism	Zea mays

mRNA and Protein(s)

mRNA	Protein	Name
NM_001112033.1	NP_001105503.1	phosphoenolpyruvate carboxylase
NM_001112033.2	NP_001105503.1	phosphoenolpyruvate carboxylase

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

The B73 maize genome: complexity, diversity, and dynamics.
Schnable PS, Ware D, Fulton RS, Stein JC, Wei F, Pasternak S, Liang C, Zhang J, Fulton L, Graves TA, Minx P, Reily AD, Courtney L, Kruchowski SS, Tomlinson C, Strong C, Delehaunty K, Fronick C, Courtney B, Rock SM, Belter E, Du F, Kim K, Abbott RM, Cotton M, Levy A, Marchetto P, Ochoa K, Jackson SM, Gillam B, Chen W, Yan L, Higginbotham J, Cardenas M, Waligorski J, Applebaum E, Phelps L, Falcone J, Kanchi K, Thane T, Scimone A, Thane N, Henke J, Wang T, Ruppert J, Shah N, Rotter K, Hodges J, Ingenthron E, Cordes M, Kohlberg S, Sgro J, Delgado B, Mead K, Chinwalla A, Leonard S, Crouse K, Collura K, Kudrna D, Currie J, He R, Angelova A, Rajasekar S, Mueller T, Lomeli R, Scara G, Ko A, Delaney K, Wissotski M, Lopez G, Campos D, Braidotti M, Ashley E, Golser W, Kim H, Lee S, Lin J, Dujmic Z, Kim W, Talag J, Zuccolo A, Fan C, Sebastian A, Kramer M, Spiegel L, Nascimento L, Zutavern T, Miller B, Ambroise C, Muller S, Spooner W, Narechania A, Ren L, Wei S, Kumari S, Faga B, Levy MJ, McMahan L, Van Buren P, Vaughn MW, Ying K, Yeh CT, Emrich SJ, Jia Y, Kalyanaraman A, Hsia AP, Barbazuk WB, Baucom RS, Brutnell TP, Carpita NC, Chaparro C, Chia JM, Deragon JM, Estill JC, Fu Y, Jeddeloh JA, Han Y, Lee H, Li P, Lisch DR, Liu S, Liu Z, Nagel DH, McCann MC, SanMiguel P, Myers AM, Nettleton D, Nguyen J, Penning BW, Ponnala L, Schneider KL, Schwartz DC, Sharma A, Soderlund C, Springer NM, Sun Q, Wang H, Waterman M, Westerman R, Wolfgruber TK, Yang L, Yu Y, Zhang L, Zhou S, Zhu Q, Bennetzen JL, Dawe RK, Jiang J, Jiang N, Presting GG, Wessler SR, Aluru S, Martienssen RA, Clifton SW, McCombie WR, Wing RA, Wilson RK
Science (New York, N.Y.)326(5956)1112-5(2009 Nov)

The regulatory role of residues 226-232 in phosphoenolpyruvate carboxylase from maize.
Yuan J, Sayegh J, Mendez J, Sward L, Sanchez N, Sanchez S, Waldrop G, Grover S
Photosynthesis research88(1)73-81(2006 Apr)

Characterization of the maize endosperm transcriptome and its comparison to the rice genome.
Lai J, Dey N, Kim CS, Bharti AK, Rudd S, Mayer KF, Larkins BA, Becraft P, Messing J
Genome research14(10A)1932-7(2004 Oct)

Phosphoenolpyruvate carboxylase prevalent in maize roots: isolation of a cDNA clone and its use for analyses of the gene and gene expression.
Kawamura T, Shigesada K, Yanagisawa S, Izui K
Journal of biochemistry107(1)165-8(1990 Jan)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following LOC542479 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC542479 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OZa05890	NM_001112033.1 Latest version!	Zea mays phosphoenolpyruvate carboxylase 7 (pep7), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
OZa70633	NM_001112033.2 Latest version!	Zea mays phosphoenolpyruvate carboxylase 7 (LOC542479), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LOC542479 ( NM_001112033.1 ) cDNA ORF clone, Zea mays -> NP_001105503.1 Zea mays phosphoenolpyruvate carboxylase 7 (pep7), mRNA.

Price & Availability↑

CloneID	OZa05890
Clone ID Related Accession (Same CDS sequence)	NM_001112033.1
Accession Version	NM_001112033.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2883bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1492531200000
Organism	Zea mays
Product	phosphoenolpyruvate carboxylase
Comment	PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AB012228.1. ##Evidence-Data-START## Transcript exon combination :: AB012228.1, SRR3147024.25154.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN01096209, SAMN03603544 [ECO:0000348] ##Evidence-Data-END##

ATGCCGGAGC GGCACCAGTC CATCGACGCG CAGCTGCGGC TGCTGGCCCC CGGGAAGGTC 
TCCGAGGACG ACAAGCTCGT CGAGTACGAC GCCCTCCTCG TCGACCGCTT CCTCGACATC 
CTCCAGGACC TGCACGGCCC CCACCTGCGC GAATTCGTGC AGGAGTGCTA CGAGCTCTCG 
GCGGAGTACG AGAACGACCG GGATGAGGCT CGGCTTGGCG AGCTCGGGAG CAAGCTCACC 
AGCCTGCCGC CGGGGGACTC CATTGTCGTC GCCAGCTCCT TCTCGCACAT GCTCAACCTC 
GCTAACCTCG CCGAGGAAGT GCAGATCGCG CACCGTCGCC GGATCAAGCT AAAGCGGGGA 
GACTTCGCCG ACGAGGCCTC CGCGCCGACG GAGTCGGACA TCGAGGAGAC GCTCAAGCGC 
CTCGTCTCTC AGCTCGGCAA GTCGCGGGAG GAGGTCTTCG ACGCGCTCAA GAACCAGACC 
GTCGACCTCG TTTTCACCGC CCACCCTACA CAGTCCGTGA GGAGGTCTCT TCTCCAGAAG 
CATGGAAGGA TCCGTAACTG CCTGAGGCAG CTTTATGCCA AGGACATCAC TGCTGACGAC 
AAGCAGGAGC TTGATGAGGC ACTTCAGAGG GAGATTCAAG CTGCTTTCAG AACTGATGAA 
ATCCGCAGAA CCCCTCCCAC TCCTCAAGAT GAAATGCGTG CTGGAATGAG TTACTTCCAT 
GAAACTATAT GGAAGGGTGT ACCAAAATTC TTGCGCCGTA TTGACACCGC TCTGAAAAAT 
ATTGGGATCA ATGAACGTCT CCCTTACAAT GCTCCTCTCA TTCAGTTCTC TTCCTGGATG 
GGTGGTGATC GTGATGGAAA TCCAAGAGTA ACACCAGAGG TTACACGGGA TGTATGCTTG 
TTGGCGAGAA TGATGGCTGC TAACTTGTAC TTCTCTCAGA TTGAAGATTT AATGTTTGAG 
CTGTCTATGT GGCGCTGCAG TGATGAACTT CGGATCCGTG CAGATGAACT ACATCGCTCT 
TCAAGAAAAG CTGCAAAGCA CTATATAGAA TTCTGGAAGC AAGTTCCGCC AAATGAACCA 
TATCGTGTCA TACTTGGTGA TGTCAGGGAT AAATTGTACT ACACACGTGA ACGTTCTCGT 
CACCTATTGA CATCTGGAAT TTCTGAGATT CTAGAGGAGG CAACTTTTAC TAATGTTGAA 
CAGTTTCTGG AACCTCTTGA GCTCTGTTAT AGATCATTAT GTGCTTGTGG TGACAAACCT 
ATAGCTGATG GAAGTCTTCT TGATTTCTTG CGTCAAGTAT CAACTTTTGG GCTTGCCCTT 
GTGAAGCTCG ACATCAGGCA GGAATCTGAT CGACACACTG ATGTCCTTGA TTCGATAACG 
ACACATCTTG GAATTGGCTC CTATGCTGAA TGGTCTGAGG AGAAACGCCA GGATTGGCTG 
TTGTCTGAAC TGAGGGGCAA ACGTCCATTG TTTGGTTCAG ATCTTCCTCA GACTGAAGAG 
ACTGCTGATG TTTTAGGCAC ATTTCATGTC CTTGCAGAGC TTCCTGCAGA TTGCTTTGGC 
GCGTATATCA TCTCTATGGC AACTGCCCCA TCCGATGTGC TTGCGGTCGA GCTTTTGCAG 
CGTGAGTGCC ATGTAAAACA TCCACTGAGA GTTGTTCCAC TTTTTGAGAA ACTTGCAGAT 
CTTGAAGCAG CTCCAGCAGC TGTAGCACGG CTCTTTTCAA TTGACTGGTA CATGGATAGG 
ATTAATGGCA AGCAGGAGGT CATGATTGGA TATTCAGACT CTGGCAAAGA TGCCGGGCGT 
CTCTCTGCAG CATGGCAAAT GTATAAAGCA CAAGAAGAGC TCATCAAAGT GGCAAAGCAT 
TATGGAGTCA AGTTGACAAT GTTTCATGGG AGGGGTGGAA CAGTTGGCAG AGGAGGTGGT 
CCAACTCATC TGGCCATATT ATCTCAGCCA CCAGACACGA TACATGGATC ACTTCGTGTA 
ACAGTTCAAG GCGAAGTTAT TGAGCACTCC TTTGGAGAGG AGCTCTTGTG CTTCAGAACT 
TTGCAACGCT ACACTGCAGC TACTCTTGAG CATGGCATGC ATCCTCCGAT TTCCCCTAAG 
CCAGAATGGC GTGCTCTGAT GGATGAAATG GCTGTTGTAG CAACTAAAGA ATATCGATCA 
ATTGTCTTCC AAGAACCACG CTTCGTTGAA TACTTCAGAT CGGCTACACC TGAGACTGAA 
TATGGTAGGA TGAATATTGG CAGCCGTCCA TCAAAGAGGA AGCCTAGTGG GGGAATAGAA 
TCACTCCGTG CAATTCCATG GATCTTTGCT TGGACACAAA CAAGGTTCCA TCTGCCTGTC 
TGGCTTGGAT TTGGTGCAGC GATAAAGCAC ATCATGCAGA AGGACATCAG GAACATCCAT 
ATACTGAGAG AAATGTACAA TGAGTGGCCA TTCTTCAGGG TCACCCTTGA TTTGCTTGAG 
ATGGTTTTCG CGAAGGGAGA CCCGGGAATC GCAGCTGTGT ACGACAAATT GCTAGTGGCT 
GATGATTTGC AATCCTTCGG CGAGCAACTG AGGAAGAACT ATGAGGAGAC AAAAGAGCTA 
CTCCTTCAGG TTGCTGGTCA CAAGGACGTC CTTGAAGGTG ATCCTTATCT GAAGCAGCGT 
CTGCGGCTGC GTGAGTCCTA CATCACCACT CTGAACGTGT GCCAGGCCTA CACCCTGAAG 
CGGATACGCG ACCCGAGCTT CCAGGTGAGC CCGCAGCCGC CCCTGTCCAA GGAGTTCACC 
GACGAGAGTC AGCCGGCGGA GCTGGTGCAG CTGAACCAGC AGAGCGAGTA CGCTCCAGGC 
CTGGAGGACA CCCTCATCCT GACCATGAAG GGCATCGCCG CCGGCATGCA GAACACCGGC 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001105503.1
CDS	198..3080
Translation	MPERHQSIDAQLRLLAPGKVSEDDKLVEYDALLVDRFLDILQDLHGPHLREFVQECYELSAEYENDRDEARLGELGSKLTSLPPGDSIVVASSFSHMLNLANLAEEVQIAHRRRIKLKRGDFADEASAPTESDIEETLKRLVSQLGKSREEVFDALKNQTVDLVFTAHPTQSVRRSLLQKHGRIRNCLRQLYAKDITADDKQELDEALQREIQAAFRTDEIRRTPPTPQDEMRAGMSYFHETIWKGVPKFLRRIDTALKNIGINERLPYNAPLIQFSSWMGGDRDGNPRVTPEVTRDVCLLARMMAANLYFSQIEDLMFELSMWRCSDELRIRADELHRSSRKAAKHYIEFWKQVPPNEPYRVILGDVRDKLYYTRERSRHLLTSGISEILEEATFTNVEQFLEPLELCYRSLCACGDKPIADGSLLDFLRQVSTFGLALVKLDIRQESDRHTDVLDSITTHLGIGSYAEWSEEKRQDWLLSELRGKRPLFGSDLPQTEETADVLGTFHVLAELPADCFGAYIISMATAPSDVLAVELLQRECHVKHPLRVVPLFEKLADLEAAPAAVARLFSIDWYMDRINGKQEVMIGYSDSGKDAGRLSAAWQMYKAQEELIKVAKHYGVKLTMFHGRGGTVGRGGGPTHLAILSQPPDTIHGSLRVTVQGEVIEHSFGEELLCFRTLQRYTAATLEHGMHPPISPKPEWRALMDEMAVVATKEYRSIVFQEPRFVEYFRSATPETEYGRMNIGSRPSKRKPSGGIESLRAIPWIFAWTQTRFHLPVWLGFGAAIKHIMQKDIRNIHILREMYNEWPFFRVTLDLLEMVFAKGDPGIAAVYDKLLVADDLQSFGEQLRKNYEETKELLLQVAGHKDVLEGDPYLKQRLRLRESYITTLNVCQAYTLKRIRDPSFQVSPQPPLSKEFTDESQPAELVQLNQQSEYAPGLEDTLILTMKGIAAGMQNTG

Target ORF information:

RefSeq Version	NM_001112033.1
Organism	Zea mays
Definition	Zea mays phosphoenolpyruvate carboxylase 7 (pep7), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001112033.1

ORF Insert Sequence:

ATGCCGGAGC GGCACCAGTC CATCGACGCG CAGCTGCGGC TGCTGGCCCC CGGGAAGGTC 
TCCGAGGACG ACAAGCTCGT CGAGTACGAC GCCCTCCTCG TCGACCGCTT CCTCGACATC 
CTCCAGGACC TGCACGGCCC CCACCTGCGC GAATTCGTGC AGGAGTGCTA CGAGCTCTCG 
GCGGAGTACG AGAACGACCG GGATGAGGCT CGGCTTGGCG AGCTCGGGAG CAAGCTCACC 
AGCCTGCCGC CGGGGGACTC CATTGTCGTC GCCAGCTCCT TCTCGCACAT GCTCAACCTC 
GCTAACCTCG CCGAGGAAGT GCAGATCGCG CACCGTCGCC GGATCAAGCT AAAGCGGGGA 
GACTTCGCCG ACGAGGCCTC CGCGCCGACG GAGTCGGACA TCGAGGAGAC GCTCAAGCGC 
CTCGTCTCTC AGCTCGGCAA GTCGCGGGAG GAGGTCTTCG ACGCGCTCAA GAACCAGACC 
GTCGACCTCG TTTTCACCGC CCACCCTACA CAGTCCGTGA GGAGGTCTCT TCTCCAGAAG 
CATGGAAGGA TCCGTAACTG CCTGAGGCAG CTTTATGCCA AGGACATCAC TGCTGACGAC 
AAGCAGGAGC TTGATGAGGC ACTTCAGAGG GAGATTCAAG CTGCTTTCAG AACTGATGAA 
ATCCGCAGAA CCCCTCCCAC TCCTCAAGAT GAAATGCGTG CTGGAATGAG TTACTTCCAT 
GAAACTATAT GGAAGGGTGT ACCAAAATTC TTGCGCCGTA TTGACACCGC TCTGAAAAAT 
ATTGGGATCA ATGAACGTCT CCCTTACAAT GCTCCTCTCA TTCAGTTCTC TTCCTGGATG 
GGTGGTGATC GTGATGGAAA TCCAAGAGTA ACACCAGAGG TTACACGGGA TGTATGCTTG 
TTGGCGAGAA TGATGGCTGC TAACTTGTAC TTCTCTCAGA TTGAAGATTT AATGTTTGAG 
CTGTCTATGT GGCGCTGCAG TGATGAACTT CGGATCCGTG CAGATGAACT ACATCGCTCT 
TCAAGAAAAG CTGCAAAGCA CTATATAGAA TTCTGGAAGC AAGTTCCGCC AAATGAACCA 
TATCGTGTCA TACTTGGTGA TGTCAGGGAT AAATTGTACT ACACACGTGA ACGTTCTCGT 
CACCTATTGA CATCTGGAAT TTCTGAGATT CTAGAGGAGG CAACTTTTAC TAATGTTGAA 
CAGTTTCTGG AACCTCTTGA GCTCTGTTAT AGATCATTAT GTGCTTGTGG TGACAAACCT 
ATAGCTGATG GAAGTCTTCT TGATTTCTTG CGTCAAGTAT CAACTTTTGG GCTTGCCCTT 
GTGAAGCTCG ACATCAGGCA GGAATCTGAT CGACACACTG ATGTCCTTGA TTCGATAACG 
ACACATCTTG GAATTGGCTC CTATGCTGAA TGGTCTGAGG AGAAACGCCA GGATTGGCTG 
TTGTCTGAAC TGAGGGGCAA ACGTCCATTG TTTGGTTCAG ATCTTCCTCA GACTGAAGAG 
ACTGCTGATG TTTTAGGCAC ATTTCATGTC CTTGCAGAGC TTCCTGCAGA TTGCTTTGGC 
GCGTATATCA TCTCTATGGC AACTGCCCCA TCCGATGTGC TTGCGGTCGA GCTTTTGCAG 
CGTGAGTGCC ATGTAAAACA TCCACTGAGA GTTGTTCCAC TTTTTGAGAA ACTTGCAGAT 
CTTGAAGCAG CTCCAGCAGC TGTAGCACGG CTCTTTTCAA TTGACTGGTA CATGGATAGG 
ATTAATGGCA AGCAGGAGGT CATGATTGGA TATTCAGACT CTGGCAAAGA TGCCGGGCGT 
CTCTCTGCAG CATGGCAAAT GTATAAAGCA CAAGAAGAGC TCATCAAAGT GGCAAAGCAT 
TATGGAGTCA AGTTGACAAT GTTTCATGGG AGGGGTGGAA CAGTTGGCAG AGGAGGTGGT 
CCAACTCATC TGGCCATATT ATCTCAGCCA CCAGACACGA TACATGGATC ACTTCGTGTA 
ACAGTTCAAG GCGAAGTTAT TGAGCACTCC TTTGGAGAGG AGCTCTTGTG CTTCAGAACT 
TTGCAACGCT ACACTGCAGC TACTCTTGAG CATGGCATGC ATCCTCCGAT TTCCCCTAAG 
CCAGAATGGC GTGCTCTGAT GGATGAAATG GCTGTTGTAG CAACTAAAGA ATATCGATCA 
ATTGTCTTCC AAGAACCACG CTTCGTTGAA TACTTCAGAT CGGCTACACC TGAGACTGAA 
TATGGTAGGA TGAATATTGG CAGCCGTCCA TCAAAGAGGA AGCCTAGTGG GGGAATAGAA 
TCACTCCGTG CAATTCCATG GATCTTTGCT TGGACACAAA CAAGGTTCCA TCTGCCTGTC 
TGGCTTGGAT TTGGTGCAGC GATAAAGCAC ATCATGCAGA AGGACATCAG GAACATCCAT 
ATACTGAGAG AAATGTACAA TGAGTGGCCA TTCTTCAGGG TCACCCTTGA TTTGCTTGAG 
ATGGTTTTCG CGAAGGGAGA CCCGGGAATC GCAGCTGTGT ACGACAAATT GCTAGTGGCT 
GATGATTTGC AATCCTTCGG CGAGCAACTG AGGAAGAACT ATGAGGAGAC AAAAGAGCTA 
CTCCTTCAGG TTGCTGGTCA CAAGGACGTC CTTGAAGGTG ATCCTTATCT GAAGCAGCGT 
CTGCGGCTGC GTGAGTCCTA CATCACCACT CTGAACGTGT GCCAGGCCTA CACCCTGAAG 
CGGATACGCG ACCCGAGCTT CCAGGTGAGC CCGCAGCCGC CCCTGTCCAA GGAGTTCACC 
GACGAGAGTC AGCCGGCGGA GCTGGTGCAG CTGAACCAGC AGAGCGAGTA CGCTCCAGGC 
CTGGAGGACA CCCTCATCCT GACCATGAAG GGCATCGCCG CCGGCATGCA GAACACCGGC 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC542479 ( NM_001112033.2 ) cDNA ORF clone, Zea mays -> NP_001105503.1 Zea mays phosphoenolpyruvate carboxylase 7 (LOC542479), mRNA.

Price & Availability↑

CloneID	OZa70633
Clone ID Related Accession (Same CDS sequence)	NM_001112033.2
Accession Version	NM_001112033.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2883bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1551715200000
Organism	Zea mays
Product	phosphoenolpyruvate carboxylase
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from EE041617.2, CD439953.1, AY109352.1, CA989174.1, CA452447.1, FK989379.1 and DN209714.1. On May 24, 2017 this sequence version replaced NM_001112033.1. ##Evidence-Data-START## Transcript exon combination :: SRR3147030.377.1, SRR3147030.3780.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN01096209, SAMN03603544 [ECO:0000348] ##Evidence-Data-END##

ATGCCGGAGC GGCACCAGTC CATCGACGCG CAGCTGCGGC TGCTGGCCCC CGGGAAGGTC 
TCCGAGGACG ACAAGCTCGT CGAGTACGAC GCCCTCCTCG TCGACCGCTT CCTCGACATC 
CTCCAGGACC TGCACGGCCC CCACCTGCGC GAATTCGTGC AGGAGTGCTA CGAGCTCTCG 
GCGGAGTACG AGAACGACCG GGATGAGGCT CGGCTTGGCG AGCTCGGGAG CAAGCTCACC 
AGCCTGCCGC CGGGGGACTC CATTGTCGTC GCCAGTTCCT TCTCGCACAT GCTCAACCTC 
GCTAACCTCG CCGAGGAAGT GCAGATCGCG CACCGTCGCC GGATCAAGCT AAAGCGGGGA 
GACTTCGCCG ACGAGGCCTC CGCGCCGACG GAGTCGGACA TCGAGGAGAC GCTCAAGCGC 
CTCGTCTCGC AGCTCGGCAA GTCGCGGGAG GAGGTCTTCG ACGCGCTCAA GAACCAGACC 
GTCGACCTCG TTTTCACCGC CCACCCTACA CAGTCCGTGA GGAGGTCTCT TCTCCAGAAG 
CATGGAAGGA TCCGTAACTG CCTGAGGCAG CTTTATGCCA AGGACATCAC TGCTGACGAC 
AAGCAGGAGC TTGATGAGGC ACTTCAGAGG GAGATTCAAG CTGCTTTCAG AACTGATGAA 
ATCCGCAGAA CCCCTCCCAC TCCTCAAGAT GAAATGCGTG CTGGAATGAG TTACTTCCAT 
GAAACTATAT GGAAGGGTGT ACCAAAATTC TTGCGCCGTA TTGACACCGC TCTGAAAAAT 
ATTGGGATCA ATGAACGTCT CCCTTACAAT GCTCCTCTCA TTCAGTTCTC TTCCTGGATG 
GGTGGTGATC GTGATGGAAA TCCAAGAGTA ACACCAGAGG TTACACGGGA TGTATGCTTG 
TTGGCGAGAA TGATGGCTGC TAACTTGTAC TTCTCTCAGA TTGAAGATTT AATGTTTGAG 
CTGTCTATGT GGCGCTGCAG TGATGAACTT CGGATCCGTG CAGATGAACT ACATCGCTCT 
TCAAGAAAAG CTGCAAAGCA CTATATAGAA TTCTGGAAGC AAGTTCCGCC AAATGAACCA 
TATCGTGTCA TACTTGGTGA TGTCAGGGAT AAATTGTACT ACACACGTGA ACGTTCTCGT 
CACCTATTGA CATCTGGAAT TTCTGAGATT CTAGAGGAGG CAACTTTTAC TAATGTTGAA 
CAGTTTCTGG AACCTCTTGA GCTCTGTTAT AGATCATTAT GTGCTTGTGG TGACAAACCT 
ATAGCTGATG GAAGTCTTCT TGATTTCTTG CGTCAAGTAT CAACTTTTGG GCTTGCCCTT 
GTGAAGCTCG ACATCAGGCA GGAATCTGAT CGACACACTG ATGTCCTTGA TTCGATAACG 
ACACATCTTG GAATTGGCTC CTATGCTGAA TGGTCTGAGG AGAAACGCCA GGATTGGCTG 
TTGTCTGAAC TGAGGGGCAA ACGTCCATTG TTTGGTTCAG ATCTTCCTCA GACTGAAGAG 
ACTGCTGATG TTTTAGGCAC ATTTCATGTC CTTGCAGAGC TTCCTGCAGA TTGCTTTGGC 
GCGTATATCA TCTCTATGGC AACTGCCCCA TCCGATGTGC TTGCGGTCGA GCTTTTGCAG 
CGTGAGTGCC ATGTAAAACA TCCACTGAGA GTTGTTCCAC TTTTTGAGAA ACTTGCAGAT 
CTTGAAGCAG CTCCAGCAGC TGTAGCACGG CTCTTTTCAA TTGACTGGTA CATGGATAGG 
ATTAATGGCA AGCAGGAGGT CATGATTGGA TATTCAGACT CTGGCAAAGA TGCCGGGCGT 
CTCTCTGCAG CATGGCAAAT GTATAAAGCA CAAGAAGAGC TCATCAAAGT GGCAAAGCAT 
TATGGAGTCA AGTTGACAAT GTTTCATGGG AGGGGTGGAA CAGTTGGCAG AGGAGGTGGT 
CCAACTCATC TGGCCATATT ATCTCAGCCA CCAGACACGA TACATGGATC ACTTCGTGTA 
ACAGTTCAAG GCGAAGTTAT TGAGCACTCC TTTGGAGAGG AGCTCTTGTG CTTCAGAACT 
TTGCAACGCT ACACTGCAGC TACTCTTGAG CATGGCATGC ATCCTCCGAT TTCCCCTAAG 
CCAGAATGGC GTGCTCTGAT GGATGAAATG GCTGTTGTAG CAACTAAAGA ATATCGATCA 
ATTGTCTTCC AAGAACCACG CTTCGTTGAA TACTTCAGAT CGGCTACACC TGAGACTGAA 
TATGGTAGGA TGAATATTGG CAGCCGTCCA TCAAAGAGGA AGCCTAGTGG GGGAATAGAA 
TCACTCCGTG CAATTCCATG GATCTTTGCT TGGACACAAA CAAGGTTCCA TCTGCCTGTC 
TGGCTTGGAT TTGGTGCAGC GATAAAGCAC ATCATGCAGA AGGACATCAG GAACATCCAT 
ATACTGAGAG AAATGTACAA TGAGTGGCCA TTCTTCAGGG TCACCCTTGA TTTGCTTGAG 
ATGGTTTTCG CGAAGGGAGA CCCGGGAATC GCAGCTGTGT ACGACAAATT GCTAGTGGCT 
GATGATTTGC AATCCTTCGG CGAGCAACTG AGGAAGAACT ATGAGGAGAC AAAAGAGCTA 
CTCCTTCAGG TTGCTGGTCA CAAGGACGTC CTTGAAGGTG ATCCTTATCT GAAGCAGCGT 
CTGCGGCTGC GTGAGTCCTA CATCACCACT CTGAACGTGT GCCAGGCCTA CACCCTGAAG 
CGGATACGCG ACCCGAGCTT CCAGGTGAGC CCGCAGCCGC CCCTGTCCAA GGAGTTCACC 
GACGAGAGTC AGCCGGCGGA GCTGGTGCAG CTGAACCAGC AGAGCGAGTA CGCTCCAGGC 
CTGGAGGACA CCCTCATCCT GACCATGAAG GGCATCGCCG CCGGCATGCA GAACACCGGC 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001105503.1
CDS	165..3047
Translation	MPERHQSIDAQLRLLAPGKVSEDDKLVEYDALLVDRFLDILQDLHGPHLREFVQECYELSAEYENDRDEARLGELGSKLTSLPPGDSIVVASSFSHMLNLANLAEEVQIAHRRRIKLKRGDFADEASAPTESDIEETLKRLVSQLGKSREEVFDALKNQTVDLVFTAHPTQSVRRSLLQKHGRIRNCLRQLYAKDITADDKQELDEALQREIQAAFRTDEIRRTPPTPQDEMRAGMSYFHETIWKGVPKFLRRIDTALKNIGINERLPYNAPLIQFSSWMGGDRDGNPRVTPEVTRDVCLLARMMAANLYFSQIEDLMFELSMWRCSDELRIRADELHRSSRKAAKHYIEFWKQVPPNEPYRVILGDVRDKLYYTRERSRHLLTSGISEILEEATFTNVEQFLEPLELCYRSLCACGDKPIADGSLLDFLRQVSTFGLALVKLDIRQESDRHTDVLDSITTHLGIGSYAEWSEEKRQDWLLSELRGKRPLFGSDLPQTEETADVLGTFHVLAELPADCFGAYIISMATAPSDVLAVELLQRECHVKHPLRVVPLFEKLADLEAAPAAVARLFSIDWYMDRINGKQEVMIGYSDSGKDAGRLSAAWQMYKAQEELIKVAKHYGVKLTMFHGRGGTVGRGGGPTHLAILSQPPDTIHGSLRVTVQGEVIEHSFGEELLCFRTLQRYTAATLEHGMHPPISPKPEWRALMDEMAVVATKEYRSIVFQEPRFVEYFRSATPETEYGRMNIGSRPSKRKPSGGIESLRAIPWIFAWTQTRFHLPVWLGFGAAIKHIMQKDIRNIHILREMYNEWPFFRVTLDLLEMVFAKGDPGIAAVYDKLLVADDLQSFGEQLRKNYEETKELLLQVAGHKDVLEGDPYLKQRLRLRESYITTLNVCQAYTLKRIRDPSFQVSPQPPLSKEFTDESQPAELVQLNQQSEYAPGLEDTLILTMKGIAAGMQNTG

Target ORF information:

RefSeq Version	NM_001112033.2
Organism	Zea mays
Definition	Zea mays phosphoenolpyruvate carboxylase 7 (LOC542479), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001112033.2

ORF Insert Sequence:

ATGCCGGAGC GGCACCAGTC CATCGACGCG CAGCTGCGGC TGCTGGCCCC CGGGAAGGTC 
TCCGAGGACG ACAAGCTCGT CGAGTACGAC GCCCTCCTCG TCGACCGCTT CCTCGACATC 
CTCCAGGACC TGCACGGCCC CCACCTGCGC GAATTCGTGC AGGAGTGCTA CGAGCTCTCG 
GCGGAGTACG AGAACGACCG GGATGAGGCT CGGCTTGGCG AGCTCGGGAG CAAGCTCACC 
AGCCTGCCGC CGGGGGACTC CATTGTCGTC GCCAGTTCCT TCTCGCACAT GCTCAACCTC 
GCTAACCTCG CCGAGGAAGT GCAGATCGCG CACCGTCGCC GGATCAAGCT AAAGCGGGGA 
GACTTCGCCG ACGAGGCCTC CGCGCCGACG GAGTCGGACA TCGAGGAGAC GCTCAAGCGC 
CTCGTCTCGC AGCTCGGCAA GTCGCGGGAG GAGGTCTTCG ACGCGCTCAA GAACCAGACC 
GTCGACCTCG TTTTCACCGC CCACCCTACA CAGTCCGTGA GGAGGTCTCT TCTCCAGAAG 
CATGGAAGGA TCCGTAACTG CCTGAGGCAG CTTTATGCCA AGGACATCAC TGCTGACGAC 
AAGCAGGAGC TTGATGAGGC ACTTCAGAGG GAGATTCAAG CTGCTTTCAG AACTGATGAA 
ATCCGCAGAA CCCCTCCCAC TCCTCAAGAT GAAATGCGTG CTGGAATGAG TTACTTCCAT 
GAAACTATAT GGAAGGGTGT ACCAAAATTC TTGCGCCGTA TTGACACCGC TCTGAAAAAT 
ATTGGGATCA ATGAACGTCT CCCTTACAAT GCTCCTCTCA TTCAGTTCTC TTCCTGGATG 
GGTGGTGATC GTGATGGAAA TCCAAGAGTA ACACCAGAGG TTACACGGGA TGTATGCTTG 
TTGGCGAGAA TGATGGCTGC TAACTTGTAC TTCTCTCAGA TTGAAGATTT AATGTTTGAG 
CTGTCTATGT GGCGCTGCAG TGATGAACTT CGGATCCGTG CAGATGAACT ACATCGCTCT 
TCAAGAAAAG CTGCAAAGCA CTATATAGAA TTCTGGAAGC AAGTTCCGCC AAATGAACCA 
TATCGTGTCA TACTTGGTGA TGTCAGGGAT AAATTGTACT ACACACGTGA ACGTTCTCGT 
CACCTATTGA CATCTGGAAT TTCTGAGATT CTAGAGGAGG CAACTTTTAC TAATGTTGAA 
CAGTTTCTGG AACCTCTTGA GCTCTGTTAT AGATCATTAT GTGCTTGTGG TGACAAACCT 
ATAGCTGATG GAAGTCTTCT TGATTTCTTG CGTCAAGTAT CAACTTTTGG GCTTGCCCTT 
GTGAAGCTCG ACATCAGGCA GGAATCTGAT CGACACACTG ATGTCCTTGA TTCGATAACG 
ACACATCTTG GAATTGGCTC CTATGCTGAA TGGTCTGAGG AGAAACGCCA GGATTGGCTG 
TTGTCTGAAC TGAGGGGCAA ACGTCCATTG TTTGGTTCAG ATCTTCCTCA GACTGAAGAG 
ACTGCTGATG TTTTAGGCAC ATTTCATGTC CTTGCAGAGC TTCCTGCAGA TTGCTTTGGC 
GCGTATATCA TCTCTATGGC AACTGCCCCA TCCGATGTGC TTGCGGTCGA GCTTTTGCAG 
CGTGAGTGCC ATGTAAAACA TCCACTGAGA GTTGTTCCAC TTTTTGAGAA ACTTGCAGAT 
CTTGAAGCAG CTCCAGCAGC TGTAGCACGG CTCTTTTCAA TTGACTGGTA CATGGATAGG 
ATTAATGGCA AGCAGGAGGT CATGATTGGA TATTCAGACT CTGGCAAAGA TGCCGGGCGT 
CTCTCTGCAG CATGGCAAAT GTATAAAGCA CAAGAAGAGC TCATCAAAGT GGCAAAGCAT 
TATGGAGTCA AGTTGACAAT GTTTCATGGG AGGGGTGGAA CAGTTGGCAG AGGAGGTGGT 
CCAACTCATC TGGCCATATT ATCTCAGCCA CCAGACACGA TACATGGATC ACTTCGTGTA 
ACAGTTCAAG GCGAAGTTAT TGAGCACTCC TTTGGAGAGG AGCTCTTGTG CTTCAGAACT 
TTGCAACGCT ACACTGCAGC TACTCTTGAG CATGGCATGC ATCCTCCGAT TTCCCCTAAG 
CCAGAATGGC GTGCTCTGAT GGATGAAATG GCTGTTGTAG CAACTAAAGA ATATCGATCA 
ATTGTCTTCC AAGAACCACG CTTCGTTGAA TACTTCAGAT CGGCTACACC TGAGACTGAA 
TATGGTAGGA TGAATATTGG CAGCCGTCCA TCAAAGAGGA AGCCTAGTGG GGGAATAGAA 
TCACTCCGTG CAATTCCATG GATCTTTGCT TGGACACAAA CAAGGTTCCA TCTGCCTGTC 
TGGCTTGGAT TTGGTGCAGC GATAAAGCAC ATCATGCAGA AGGACATCAG GAACATCCAT 
ATACTGAGAG AAATGTACAA TGAGTGGCCA TTCTTCAGGG TCACCCTTGA TTTGCTTGAG 
ATGGTTTTCG CGAAGGGAGA CCCGGGAATC GCAGCTGTGT ACGACAAATT GCTAGTGGCT 
GATGATTTGC AATCCTTCGG CGAGCAACTG AGGAAGAACT ATGAGGAGAC AAAAGAGCTA 
CTCCTTCAGG TTGCTGGTCA CAAGGACGTC CTTGAAGGTG ATCCTTATCT GAAGCAGCGT 
CTGCGGCTGC GTGAGTCCTA CATCACCACT CTGAACGTGT GCCAGGCCTA CACCCTGAAG 
CGGATACGCG ACCCGAGCTT CCAGGTGAGC CCGCAGCCGC CCCTGTCCAA GGAGTTCACC 
GACGAGAGTC AGCCGGCGGA GCTGGTGCAG CTGAACCAGC AGAGCGAGTA CGCTCCAGGC 
CTGGAGGACA CCCTCATCCT GACCATGAAG GGCATCGCCG CCGGCATGCA GAACACCGGC 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The B73 maize genome: complexity, diversity, and dynamics.
Science (New York, N.Y.)326(5956)1112-5(2009 Nov)
Schnable PS,Ware D,Fulton RS,Stein JC,Wei F,Pasternak S,Liang C,Zhang J,Fulton L,Graves TA,Minx P,Reily AD,Courtney L,Kruchowski SS,Tomlinson C,Strong C,Delehaunty K,Fronick C,Courtney B,Rock SM,Belter E,Du F,Kim K,Abbott RM,Cotton M,Levy A,Marchetto P,Ochoa K,Jackson SM,Gillam B,Chen W,Yan L,Higginbotham J,Cardenas M,Waligorski J,Applebaum E,Phelps L,Falcone J,Kanchi K,Thane T,Scimone A,Thane N,Henke J,Wang T,Ruppert J,Shah N,Rotter K,Hodges J,Ingenthron E,Cordes M,Kohlberg S,Sgro J,Delgado B,Mead K,Chinwalla A,Leonard S,Crouse K,Collura K,Kudrna D,Currie J,He R,Angelova A,Rajasekar S,Mueller T,Lomeli R,Scara G,Ko A,Delaney K,Wissotski M,Lopez G,Campos D,Braidotti M,Ashley E,Golser W,Kim H,Lee S,Lin J,Dujmic Z,Kim W,Talag J,Zuccolo A,Fan C,Sebastian A,Kramer M,Spiegel L,Nascimento L,Zutavern T,Miller B,Ambroise C,Muller S,Spooner W,Narechania A,Ren L,Wei S,Kumari S,Faga B,Levy MJ,McMahan L,Van Buren P,Vaughn MW,Ying K,Yeh CT,Emrich SJ,Jia Y,Kalyanaraman A,Hsia AP,Barbazuk WB,Baucom RS,Brutnell TP,Carpita NC,Chaparro C,Chia JM,Deragon JM,Estill JC,Fu Y,Jeddeloh JA,Han Y,Lee H,Li P,Lisch DR,Liu S,Liu Z,Nagel DH,McCann MC,SanMiguel P,Myers AM,Nettleton D,Nguyen J,Penning BW,Ponnala L,Schneider KL,Schwartz DC,Sharma A,Soderlund C,Springer NM,Sun Q,Wang H,Waterman M,Westerman R,Wolfgruber TK,Yang L,Yu Y,Zhang L,Zhou S,Zhu Q,Bennetzen JL,Dawe RK,Jiang J,Jiang N,Presting GG,Wessler SR,Aluru S,Martienssen RA,Clifton SW,McCombie WR,Wing RA,Wilson RK

The regulatory role of residues 226-232 in phosphoenolpyruvate carboxylase from maize.
Photosynthesis research88(1)73-81(2006 Apr)
Yuan J,Sayegh J,Mendez J,Sward L,Sanchez N,Sanchez S,Waldrop G,Grover S

Characterization of the maize endosperm transcriptome and its comparison to the rice genome.
Genome research14(10A)1932-7(2004 Oct)
Lai J,Dey N,Kim CS,Bharti AK,Rudd S,Mayer KF,Larkins BA,Becraft P,Messing J

Phosphoenolpyruvate carboxylase prevalent in maize roots: isolation of a cDNA clone and its use for analyses of the gene and gene expression.
Journal of biochemistry107(1)165-8(1990 Jan)
Kawamura T,Shigesada K,Yanagisawa S,Izui K

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LOC542479 cDNA ORF clone, Zea mays

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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