LOC103639062 cDNA ORF clone, Zea mays

Gene
Clones
gRNAs

Summary

Gene Symbol	LOC103639062
Entrez Gene ID	103639062
Full Name	pentatricopeptide repeat-containing protein At4g31850, chloroplastic
Synonyms	GRMZM2G372632
Gene Type	protein-coding
Organism	Zea mays

mRNA and Protein(s)

mRNA	Protein	Name
XM_008661857.3	XP_008660079.1	pentatricopeptide repeat-containing protein At4g31850, chloroplastic
XM_008661857.2	XP_008660079.1	pentatricopeptide repeat-containing protein At4g31850, chloroplastic

Pathways from BioSystems
Gene Ontology

The following LOC103639062 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC103639062 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OZa41499	XM_008661857.3 Latest version!	Zea mays pentatricopeptide repeat-containing protein At4g31850, chloroplastic (LOC103639062), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
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OZa41499	XM_008661857.2	Zea mays pentatricopeptide repeat-containing protein At4g31850, chloroplastic (LOC103639062), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LOC103639062 ( XM_008661857.3 ) cDNA ORF clone, Zea mays -> XP_008660079.1 Zea mays pentatricopeptide repeat-containing protein At4g31850, chloroplastic (LOC103639062), mRNA.

Price & Availability↑

CloneID	OZa41499
Clone ID Related Accession (Same CDS sequence)	XM_008661857.3 , XM_008661857.2
Accession Version	XM_008661857.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3276bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1513440000000
Organism	Zea mays
Product	pentatricopeptide repeat-containing protein At4g31850, chloroplastic
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_024467.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2017 this sequence version replaced XM_008661857.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Zea mays Annotation Release 102 Annotation Version :: 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGCTCT GCTGCTCAGG CGTCCTAAGC GGCGCCGGCG CCTCGCGACC GGCGCCATCG 
AGGACCTCAA GGCCCGTGAA AATGGGGGCT CCGTCCACTG GGCTCTTGGT AGCCCCGCCG 
AAGCGGCGGC GAGGCGGCCG CGCCGGCTGC CGCCAGTCGG CCCCGCCAGT GCCGCCCCGG 
GACGAACGGC GGGTCGTGGG CACGGACAGC GTCGTCCACA TGCTCCGGTC GGCGCCCGAC 
CCCGCCGAGG CCCTGGAACT CTTCACGGCC GCGGCGCGGC AGCCCACGAA GGTCCACACA 
ACGGAGTCGT GCAACTACAT GCTGGAGCTG ATGCGCGCCC ACGGTCGTGT CGGGGACATG 
GCGCAGGTGT TCGACCTAAT GCAGAAACAG GTCGTCAAGA CCAACGTCGG CACCTTCGCC 
ACCATCTTCG GCGGCGTCGG CGTCGAGGGG GGGCTCCGCA GCGCGCCGGT CGCCCTGCCT 
GTGATGAGGG AGGCGGGGAT GTCCCTCAAC GCCTACACGT ACAATGGCTT GATTTACTTC 
CTCGTTAAGT CCGGCTTCGA TGCGGAGGCC ATGGAGGTTT ACAAGGCGAT GGTTGAGGAC 
GGCATCTCGC CGAGTGTGAG GACCTACTCC GTGCTGATGG TGGCGTTTGG GAAGAAGAGG 
GATGTCGACA CTGTTCTCTG GTTGCTGAAC GAGATGGAGG CCCGCGGCGT CAAGCCAAAC 
GTGTATAGCT ACACCATCTG CATTCGGGTT CTTGGGCAGG CCGCAAGGTT TGATGAAGCA 
TATCACATTC TTGGAAAGAT GGAGGATTCA GGGTGCAAAC CAGATGTCGT CACACATACT 
GTGATTATAC AGGTTCTCTG TGATGCTGGC CGTCTGAGCG ACGCCAAGGC TGTCTTTTGG 
AAGATGAAAG CGAGTGATCA GAAACCTGAT CGTGTCACTT ACATCACTCT CTTAGACAAG 
TGTGGTGACA GTGGTGACTC ACAATCAGTT GTCGAAGTTT GGAATGCCAT GGTAGCTGAT 
GGTTATAATG ACAATATTGT TTCTTACACT GCAGTTGTGG ATGCATTATG CCAAGTTGGC 
CGGGTTGATG AAGCTTTAGC TGTGTTTGAT GAGATGAAGG AAAAGGGTAT GTCGCCTGAG 
CAGTATTCCT ATAACTCCTT AATATCTGGG TTTCTGAAAG CTGATATGTT TGACCGTGCT 
CTGGAGCTCT TCAACCATAT GAATGCTTGT GGTCCTAGTC CAAATGGCTA CACTCATGTC 
CTCTTCATCA ACTACTACGG AAAATCTGGT CAATCTTTGA AAGCAATACA AAGATATGAG 
CACATGAAGA GCAAAGGGAT TGTTCCTGAT GTTGCTGCTG CTAATGCTGT TCTGTATAGT 
CTTGCTGGAT CTGGCAGACT TGGAATGGCA AAAAGGGTCT TTTATGAACT AAAAGCGATG 
GGAGTCTCTC CAGACACTAT CACGTACACA ATGATGATCA AATGTTGCAG TAAGGCATCA 
AAAGCTGATG AAGCTATGAA TTTTTTTTCT GACATGGTGG AAAGTGGATG TGTTCCTGAT 
GTTCTTGCAT TGAATTCATT GATTGATACA CTCTACAAGG GAGGCAAGGG AAACGAAGCA 
TGGCAACTTT TCCATAAATT GAAAGAAATG AAAATTGAGC CCACTAATGG TACGTACAAC 
ACACTTTTGT CAGGATTAGG AAGAGAAGGC AAAGTTAAGG AGGTAATGCA GCTGCTCGAA 
GAAATGACAC GTACCATTTA TCCACCTAAT TTAATTACAT ACAACACAGT TCTAGATTGC 
CTCAGCAAAA ATGGGGAGGT GAACTGTGCA ATAGATATGC TTTACAGTAT GACTGAGAAA 
GGTTGCGCAC CTGACCTTTC ATCTTATAAC ACTGTCATGT ATGGCCTTAT TAAAGAGGAA 
AGACTTGAAG AGGCATTCAG GATGTTTTGT CAAATGAAAA AGATTCTTGC TCCAGATTAT 
GCCACACTGT GTACTATCCT CCCAAGTTTT GTTAAAAATG GGTTGATGAA GGAAGCTCTG 
CATACTGTAA AGGAATATAT TTTGAAGGCT GGTTGTAACG TGGACAAGTC TTCATTCCAT 
TCACTGATGG AAGGGATACT GAAGAAGGCT GGTGTGGAAA AATCAATCGA GTTTGCTGAA 
AACATAGCAT CTAGGGGAAT TCTGCTGAAT GATTTCTTTT TATGCCCATT GATTAGACAC 
CTCTGCAAGC ACAAGAAAGC TCTTGAAGCG CATCAACTAT TCAATAAGTT TAAAGGTCTT 
GGAGTTTCAC TAAAAACTGG TTCTTATAAT TCTCTTATCC GTGGCCTTGT GGATGAAAAC 
CTGATTGATA TCGCTGAAGA CTTGTTCACT GAAATGAAAA GACTAGGATG TGGCCCAGAT 
GAATTCACTT ACAATTTGAT TCTTGATGCC ATGGGAAAAT CGATGCGGGT TGAGGAAATG 
TTAAAAGTCC AAAAGGAGAT GCATCGTAAG GGATATGAAT CAACATATGT TACTTATAAC 
ACTATCATTT CGGGTCTCGT AAAATCTAAA AGATTGGAAC AGGCTATAGA CCTGTACTAC 
AATCTGATGA GTGAAGGCTT CTCCCCAACA CCATGTACAT ATGGTCCTCT TCTTGATGGC 
TTATTAAAAT CTGGAAAGAT GGTGGATGCT GAAAATCTTT TTAATGAGAT GCTAGAGTAT 
GGATGCGAAC CTAATTGCAC CATCTACAAT ATTCTACTGA ATGGACACCG AATAGCTGGC 
AACACAGAAA ACGTTTGTCA GCTTTTTGAA AAGATGGTTG AGCAGGGAAT AAACCCAGAT 
ATAAAATCCT ATACAATTCT TATTGATACC CTGTGCACAG CTGGAAGATT AAATGATGGT 
TTGTGTTATT TTAGACAGCT ACATGAATTA GGACTTGAGC CTGATCTTAT CGTTTATAAT 
TTGCTCATTG ATGGTCTTGG AAAATCTGAA AGAATAGAGG AAGCAGTCAG TCTCTTCAAC 
GAGATGAAGA AGAAAGGAAT TATCCCAAAC TTATACACAT ACAATTCACT AATTCTCCAC 
TTAGGGAAAG CAGGGAAAGC TGCTGAAGCT GCGCAAATGT ATGAAGAACT ACTGAGAAAA 
GGATGGAAAC CTAGTGTTTT CACATATAAT GCACTCATCA GGGGTTACAG TGTTTCTGGC 
AGTACCGATA ATGCATACGC TGCCTATGGT CAGATGATTG TTGGAGGGTG CCAGCCTAAT 
TCTAGCACTT ATATGCAACT TCCAAATCAA TTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008660079.1
CDS	452..3727
Translation	MELCCSGVLSGAGASRPAPSRTSRPVKMGAPSTGLLVAPPKRRRGGRAGCRQSAPPVPPRDERRVVGTDSVVHMLRSAPDPAEALELFTAAARQPTKVHTTESCNYMLELMRAHGRVGDMAQVFDLMQKQVVKTNVGTFATIFGGVGVEGGLRSAPVALPVMREAGMSLNAYTYNGLIYFLVKSGFDAEAMEVYKAMVEDGISPSVRTYSVLMVAFGKKRDVDTVLWLLNEMEARGVKPNVYSYTICIRVLGQAARFDEAYHILGKMEDSGCKPDVVTHTVIIQVLCDAGRLSDAKAVFWKMKASDQKPDRVTYITLLDKCGDSGDSQSVVEVWNAMVADGYNDNIVSYTAVVDALCQVGRVDEALAVFDEMKEKGMSPEQYSYNSLISGFLKADMFDRALELFNHMNACGPSPNGYTHVLFINYYGKSGQSLKAIQRYEHMKSKGIVPDVAAANAVLYSLAGSGRLGMAKRVFYELKAMGVSPDTITYTMMIKCCSKASKADEAMNFFSDMVESGCVPDVLALNSLIDTLYKGGKGNEAWQLFHKLKEMKIEPTNGTYNTLLSGLGREGKVKEVMQLLEEMTRTIYPPNLITYNTVLDCLSKNGEVNCAIDMLYSMTEKGCAPDLSSYNTVMYGLIKEERLEEAFRMFCQMKKILAPDYATLCTILPSFVKNGLMKEALHTVKEYILKAGCNVDKSSFHSLMEGILKKAGVEKSIEFAENIASRGILLNDFFLCPLIRHLCKHKKALEAHQLFNKFKGLGVSLKTGSYNSLIRGLVDENLIDIAEDLFTEMKRLGCGPDEFTYNLILDAMGKSMRVEEMLKVQKEMHRKGYESTYVTYNTIISGLVKSKRLEQAIDLYYNLMSEGFSPTPCTYGPLLDGLLKSGKMVDAENLFNEMLEYGCEPNCTIYNILLNGHRIAGNTENVCQLFEKMVEQGINPDIKSYTILIDTLCTAGRLNDGLCYFRQLHELGLEPDLIVYNLLIDGLGKSERIEEAVSLFNEMKKKGIIPNLYTYNSLILHLGKAGKAAEAAQMYEELLRKGWKPSVFTYNALIRGYSVSGSTDNAYAAYGQMIVGGCQPNSSTYMQLPNQL

Target ORF information:

RefSeq Version	XM_008661857.3
Organism	Zea mays
Definition	Zea mays pentatricopeptide repeat-containing protein At4g31850, chloroplastic (LOC103639062), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008661857.3

ORF Insert Sequence:

ATGGAGCTCT GCTGCTCAGG CGTCCTAAGC GGCGCCGGCG CCTCGCGACC GGCGCCATCG 
AGGACCTCAA GGCCCGTGAA AATGGGGGCT CCGTCCACTG GGCTCTTGGT AGCCCCGCCG 
AAGCGGCGGC GAGGCGGCCG CGCCGGCTGC CGCCAGTCGG CCCCGCCAGT GCCGCCCCGG 
GACGAACGGC GGGTCGTGGG CACGGACAGC GTCGTCCACA TGCTCCGGTC GGCGCCCGAC 
CCCGCCGAGG CCCTGGAACT CTTCACGGCC GCGGCGCGGC AGCCCACGAA GGTCCACACA 
ACGGAGTCGT GCAACTACAT GCTGGAGCTG ATGCGCGCCC ACGGTCGTGT CGGGGACATG 
GCGCAGGTGT TCGACCTAAT GCAGAAACAG GTCGTCAAGA CCAACGTCGG CACCTTCGCC 
ACCATCTTCG GCGGCGTCGG CGTCGAGGGG GGGCTCCGCA GCGCGCCGGT CGCCCTGCCT 
GTGATGAGGG AGGCGGGGAT GTCCCTCAAC GCCTACACGT ACAATGGCTT GATTTACTTC 
CTCGTTAAGT CCGGCTTCGA TGCGGAGGCC ATGGAGGTTT ACAAGGCGAT GGTTGAGGAC 
GGCATCTCGC CGAGTGTGAG GACCTACTCC GTGCTGATGG TGGCGTTTGG GAAGAAGAGG 
GATGTCGACA CTGTTCTCTG GTTGCTGAAC GAGATGGAGG CCCGCGGCGT CAAGCCAAAC 
GTGTATAGCT ACACCATCTG CATTCGGGTT CTTGGGCAGG CCGCAAGGTT TGATGAAGCA 
TATCACATTC TTGGAAAGAT GGAGGATTCA GGGTGCAAAC CAGATGTCGT CACACATACT 
GTGATTATAC AGGTTCTCTG TGATGCTGGC CGTCTGAGCG ACGCCAAGGC TGTCTTTTGG 
AAGATGAAAG CGAGTGATCA GAAACCTGAT CGTGTCACTT ACATCACTCT CTTAGACAAG 
TGTGGTGACA GTGGTGACTC ACAATCAGTT GTCGAAGTTT GGAATGCCAT GGTAGCTGAT 
GGTTATAATG ACAATATTGT TTCTTACACT GCAGTTGTGG ATGCATTATG CCAAGTTGGC 
CGGGTTGATG AAGCTTTAGC TGTGTTTGAT GAGATGAAGG AAAAGGGTAT GTCGCCTGAG 
CAGTATTCCT ATAACTCCTT AATATCTGGG TTTCTGAAAG CTGATATGTT TGACCGTGCT 
CTGGAGCTCT TCAACCATAT GAATGCTTGT GGTCCTAGTC CAAATGGCTA CACTCATGTC 
CTCTTCATCA ACTACTACGG AAAATCTGGT CAATCTTTGA AAGCAATACA AAGATATGAG 
CACATGAAGA GCAAAGGGAT TGTTCCTGAT GTTGCTGCTG CTAATGCTGT TCTGTATAGT 
CTTGCTGGAT CTGGCAGACT TGGAATGGCA AAAAGGGTCT TTTATGAACT AAAAGCGATG 
GGAGTCTCTC CAGACACTAT CACGTACACA ATGATGATCA AATGTTGCAG TAAGGCATCA 
AAAGCTGATG AAGCTATGAA TTTTTTTTCT GACATGGTGG AAAGTGGATG TGTTCCTGAT 
GTTCTTGCAT TGAATTCATT GATTGATACA CTCTACAAGG GAGGCAAGGG AAACGAAGCA 
TGGCAACTTT TCCATAAATT GAAAGAAATG AAAATTGAGC CCACTAATGG TACGTACAAC 
ACACTTTTGT CAGGATTAGG AAGAGAAGGC AAAGTTAAGG AGGTAATGCA GCTGCTCGAA 
GAAATGACAC GTACCATTTA TCCACCTAAT TTAATTACAT ACAACACAGT TCTAGATTGC 
CTCAGCAAAA ATGGGGAGGT GAACTGTGCA ATAGATATGC TTTACAGTAT GACTGAGAAA 
GGTTGCGCAC CTGACCTTTC ATCTTATAAC ACTGTCATGT ATGGCCTTAT TAAAGAGGAA 
AGACTTGAAG AGGCATTCAG GATGTTTTGT CAAATGAAAA AGATTCTTGC TCCAGATTAT 
GCCACACTGT GTACTATCCT CCCAAGTTTT GTTAAAAATG GGTTGATGAA GGAAGCTCTG 
CATACTGTAA AGGAATATAT TTTGAAGGCT GGTTGTAACG TGGACAAGTC TTCATTCCAT 
TCACTGATGG AAGGGATACT GAAGAAGGCT GGTGTGGAAA AATCAATCGA GTTTGCTGAA 
AACATAGCAT CTAGGGGAAT TCTGCTGAAT GATTTCTTTT TATGCCCATT GATTAGACAC 
CTCTGCAAGC ACAAGAAAGC TCTTGAAGCG CATCAACTAT TCAATAAGTT TAAAGGTCTT 
GGAGTTTCAC TAAAAACTGG TTCTTATAAT TCTCTTATCC GTGGCCTTGT GGATGAAAAC 
CTGATTGATA TCGCTGAAGA CTTGTTCACT GAAATGAAAA GACTAGGATG TGGCCCAGAT 
GAATTCACTT ACAATTTGAT TCTTGATGCC ATGGGAAAAT CGATGCGGGT TGAGGAAATG 
TTAAAAGTCC AAAAGGAGAT GCATCGTAAG GGATATGAAT CAACATATGT TACTTATAAC 
ACTATCATTT CGGGTCTCGT AAAATCTAAA AGATTGGAAC AGGCTATAGA CCTGTACTAC 
AATCTGATGA GTGAAGGCTT CTCCCCAACA CCATGTACAT ATGGTCCTCT TCTTGATGGC 
TTATTAAAAT CTGGAAAGAT GGTGGATGCT GAAAATCTTT TTAATGAGAT GCTAGAGTAT 
GGATGCGAAC CTAATTGCAC CATCTACAAT ATTCTACTGA ATGGACACCG AATAGCTGGC 
AACACAGAAA ACGTTTGTCA GCTTTTTGAA AAGATGGTTG AGCAGGGAAT AAACCCAGAT 
ATAAAATCCT ATACAATTCT TATTGATACC CTGTGCACAG CTGGAAGATT AAATGATGGT 
TTGTGTTATT TTAGACAGCT ACATGAATTA GGACTTGAGC CTGATCTTAT CGTTTATAAT 
TTGCTCATTG ATGGTCTTGG AAAATCTGAA AGAATAGAGG AAGCAGTCAG TCTCTTCAAC 
GAGATGAAGA AGAAAGGAAT TATCCCAAAC TTATACACAT ACAATTCACT AATTCTCCAC 
TTAGGGAAAG CAGGGAAAGC TGCTGAAGCT GCGCAAATGT ATGAAGAACT ACTGAGAAAA 
GGATGGAAAC CTAGTGTTTT CACATATAAT GCACTCATCA GGGGTTACAG TGTTTCTGGC 
AGTACCGATA ATGCATACGC TGCCTATGGT CAGATGATTG TTGGAGGGTG CCAGCCTAAT 
TCTAGCACTT ATATGCAACT TCCAAATCAA TTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC103639062 ( XM_008661857.2 ) cDNA ORF clone, Zea mays -> XP_008660079.1 Zea mays pentatricopeptide repeat-containing protein At4g31850, chloroplastic (LOC103639062), mRNA.

Price & Availability↑

CloneID	OZa41499
Clone ID Related Accession (Same CDS sequence)	XM_008661857.3 , XM_008661857.2
Accession Version	XM_008661857.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3276bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1489852800000
Organism	Zea mays
Product	pentatricopeptide repeat-containing protein At4g31850, chloroplastic
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_024467.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 20, 2017 this sequence version replaced XM_008661857.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Zea mays Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGCTCT GCTGCTCAGG CGTCCTAAGC GGCGCCGGCG CCTCGCGACC GGCGCCATCG 
AGGACCTCAA GGCCCGTGAA AATGGGGGCT CCGTCCACTG GGCTCTTGGT AGCCCCGCCG 
AAGCGGCGGC GAGGCGGCCG CGCCGGCTGC CGCCAGTCGG CCCCGCCAGT GCCGCCCCGG 
GACGAACGGC GGGTCGTGGG CACGGACAGC GTCGTCCACA TGCTCCGGTC GGCGCCCGAC 
CCCGCCGAGG CCCTGGAACT CTTCACGGCC GCGGCGCGGC AGCCCACGAA GGTCCACACA 
ACGGAGTCGT GCAACTACAT GCTGGAGCTG ATGCGCGCCC ACGGTCGTGT CGGGGACATG 
GCGCAGGTGT TCGACCTAAT GCAGAAACAG GTCGTCAAGA CCAACGTCGG CACCTTCGCC 
ACCATCTTCG GCGGCGTCGG CGTCGAGGGG GGGCTCCGCA GCGCGCCGGT CGCCCTGCCT 
GTGATGAGGG AGGCGGGGAT GTCCCTCAAC GCCTACACGT ACAATGGCTT GATTTACTTC 
CTCGTTAAGT CCGGCTTCGA TGCGGAGGCC ATGGAGGTTT ACAAGGCGAT GGTTGAGGAC 
GGCATCTCGC CGAGTGTGAG GACCTACTCC GTGCTGATGG TGGCGTTTGG GAAGAAGAGG 
GATGTCGACA CTGTTCTCTG GTTGCTGAAC GAGATGGAGG CCCGCGGCGT CAAGCCAAAC 
GTGTATAGCT ACACCATCTG CATTCGGGTT CTTGGGCAGG CCGCAAGGTT TGATGAAGCA 
TATCACATTC TTGGAAAGAT GGAGGATTCA GGGTGCAAAC CAGATGTCGT CACACATACT 
GTGATTATAC AGGTTCTCTG TGATGCTGGC CGTCTGAGCG ACGCCAAGGC TGTCTTTTGG 
AAGATGAAAG CGAGTGATCA GAAACCTGAT CGTGTCACTT ACATCACTCT CTTAGACAAG 
TGTGGTGACA GTGGTGACTC ACAATCAGTT GTCGAAGTTT GGAATGCCAT GGTAGCTGAT 
GGTTATAATG ACAATATTGT TTCTTACACT GCAGTTGTGG ATGCATTATG CCAAGTTGGC 
CGGGTTGATG AAGCTTTAGC TGTGTTTGAT GAGATGAAGG AAAAGGGTAT GTCGCCTGAG 
CAGTATTCCT ATAACTCCTT AATATCTGGG TTTCTGAAAG CTGATATGTT TGACCGTGCT 
CTGGAGCTCT TCAACCATAT GAATGCTTGT GGTCCTAGTC CAAATGGCTA CACTCATGTC 
CTCTTCATCA ACTACTACGG AAAATCTGGT CAATCTTTGA AAGCAATACA AAGATATGAG 
CACATGAAGA GCAAAGGGAT TGTTCCTGAT GTTGCTGCTG CTAATGCTGT TCTGTATAGT 
CTTGCTGGAT CTGGCAGACT TGGAATGGCA AAAAGGGTCT TTTATGAACT AAAAGCGATG 
GGAGTCTCTC CAGACACTAT CACGTACACA ATGATGATCA AATGTTGCAG TAAGGCATCA 
AAAGCTGATG AAGCTATGAA TTTTTTTTCT GACATGGTGG AAAGTGGATG TGTTCCTGAT 
GTTCTTGCAT TGAATTCATT GATTGATACA CTCTACAAGG GAGGCAAGGG AAACGAAGCA 
TGGCAACTTT TCCATAAATT GAAAGAAATG AAAATTGAGC CCACTAATGG TACGTACAAC 
ACACTTTTGT CAGGATTAGG AAGAGAAGGC AAAGTTAAGG AGGTAATGCA GCTGCTCGAA 
GAAATGACAC GTACCATTTA TCCACCTAAT TTAATTACAT ACAACACAGT TCTAGATTGC 
CTCAGCAAAA ATGGGGAGGT GAACTGTGCA ATAGATATGC TTTACAGTAT GACTGAGAAA 
GGTTGCGCAC CTGACCTTTC ATCTTATAAC ACTGTCATGT ATGGCCTTAT TAAAGAGGAA 
AGACTTGAAG AGGCATTCAG GATGTTTTGT CAAATGAAAA AGATTCTTGC TCCAGATTAT 
GCCACACTGT GTACTATCCT CCCAAGTTTT GTTAAAAATG GGTTGATGAA GGAAGCTCTG 
CATACTGTAA AGGAATATAT TTTGAAGGCT GGTTGTAACG TGGACAAGTC TTCATTCCAT 
TCACTGATGG AAGGGATACT GAAGAAGGCT GGTGTGGAAA AATCAATCGA GTTTGCTGAA 
AACATAGCAT CTAGGGGAAT TCTGCTGAAT GATTTCTTTT TATGCCCATT GATTAGACAC 
CTCTGCAAGC ACAAGAAAGC TCTTGAAGCG CATCAACTAT TCAATAAGTT TAAAGGTCTT 
GGAGTTTCAC TAAAAACTGG TTCTTATAAT TCTCTTATCC GTGGCCTTGT GGATGAAAAC 
CTGATTGATA TCGCTGAAGA CTTGTTCACT GAAATGAAAA GACTAGGATG TGGCCCAGAT 
GAATTCACTT ACAATTTGAT TCTTGATGCC ATGGGAAAAT CGATGCGGGT TGAGGAAATG 
TTAAAAGTCC AAAAGGAGAT GCATCGTAAG GGATATGAAT CAACATATGT TACTTATAAC 
ACTATCATTT CGGGTCTCGT AAAATCTAAA AGATTGGAAC AGGCTATAGA CCTGTACTAC 
AATCTGATGA GTGAAGGCTT CTCCCCAACA CCATGTACAT ATGGTCCTCT TCTTGATGGC 
TTATTAAAAT CTGGAAAGAT GGTGGATGCT GAAAATCTTT TTAATGAGAT GCTAGAGTAT 
GGATGCGAAC CTAATTGCAC CATCTACAAT ATTCTACTGA ATGGACACCG AATAGCTGGC 
AACACAGAAA ACGTTTGTCA GCTTTTTGAA AAGATGGTTG AGCAGGGAAT AAACCCAGAT 
ATAAAATCCT ATACAATTCT TATTGATACC CTGTGCACAG CTGGAAGATT AAATGATGGT 
TTGTGTTATT TTAGACAGCT ACATGAATTA GGACTTGAGC CTGATCTTAT CGTTTATAAT 
TTGCTCATTG ATGGTCTTGG AAAATCTGAA AGAATAGAGG AAGCAGTCAG TCTCTTCAAC 
GAGATGAAGA AGAAAGGAAT TATCCCAAAC TTATACACAT ACAATTCACT AATTCTCCAC 
TTAGGGAAAG CAGGGAAAGC TGCTGAAGCT GCGCAAATGT ATGAAGAACT ACTGAGAAAA 
GGATGGAAAC CTAGTGTTTT CACATATAAT GCACTCATCA GGGGTTACAG TGTTTCTGGC 
AGTACCGATA ATGCATACGC TGCCTATGGT CAGATGATTG TTGGAGGGTG CCAGCCTAAT 
TCTAGCACTT ATATGCAACT TCCAAATCAA TTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008660079.1
CDS	455..3730
Translation	MELCCSGVLSGAGASRPAPSRTSRPVKMGAPSTGLLVAPPKRRRGGRAGCRQSAPPVPPRDERRVVGTDSVVHMLRSAPDPAEALELFTAAARQPTKVHTTESCNYMLELMRAHGRVGDMAQVFDLMQKQVVKTNVGTFATIFGGVGVEGGLRSAPVALPVMREAGMSLNAYTYNGLIYFLVKSGFDAEAMEVYKAMVEDGISPSVRTYSVLMVAFGKKRDVDTVLWLLNEMEARGVKPNVYSYTICIRVLGQAARFDEAYHILGKMEDSGCKPDVVTHTVIIQVLCDAGRLSDAKAVFWKMKASDQKPDRVTYITLLDKCGDSGDSQSVVEVWNAMVADGYNDNIVSYTAVVDALCQVGRVDEALAVFDEMKEKGMSPEQYSYNSLISGFLKADMFDRALELFNHMNACGPSPNGYTHVLFINYYGKSGQSLKAIQRYEHMKSKGIVPDVAAANAVLYSLAGSGRLGMAKRVFYELKAMGVSPDTITYTMMIKCCSKASKADEAMNFFSDMVESGCVPDVLALNSLIDTLYKGGKGNEAWQLFHKLKEMKIEPTNGTYNTLLSGLGREGKVKEVMQLLEEMTRTIYPPNLITYNTVLDCLSKNGEVNCAIDMLYSMTEKGCAPDLSSYNTVMYGLIKEERLEEAFRMFCQMKKILAPDYATLCTILPSFVKNGLMKEALHTVKEYILKAGCNVDKSSFHSLMEGILKKAGVEKSIEFAENIASRGILLNDFFLCPLIRHLCKHKKALEAHQLFNKFKGLGVSLKTGSYNSLIRGLVDENLIDIAEDLFTEMKRLGCGPDEFTYNLILDAMGKSMRVEEMLKVQKEMHRKGYESTYVTYNTIISGLVKSKRLEQAIDLYYNLMSEGFSPTPCTYGPLLDGLLKSGKMVDAENLFNEMLEYGCEPNCTIYNILLNGHRIAGNTENVCQLFEKMVEQGINPDIKSYTILIDTLCTAGRLNDGLCYFRQLHELGLEPDLIVYNLLIDGLGKSERIEEAVSLFNEMKKKGIIPNLYTYNSLILHLGKAGKAAEAAQMYEELLRKGWKPSVFTYNALIRGYSVSGSTDNAYAAYGQMIVGGCQPNSSTYMQLPNQL

Target ORF information:

RefSeq Version	XM_008661857.2
Organism	Zea mays
Definition	Zea mays pentatricopeptide repeat-containing protein At4g31850, chloroplastic (LOC103639062), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008661857.2

ORF Insert Sequence:

ATGGAGCTCT GCTGCTCAGG CGTCCTAAGC GGCGCCGGCG CCTCGCGACC GGCGCCATCG 
AGGACCTCAA GGCCCGTGAA AATGGGGGCT CCGTCCACTG GGCTCTTGGT AGCCCCGCCG 
AAGCGGCGGC GAGGCGGCCG CGCCGGCTGC CGCCAGTCGG CCCCGCCAGT GCCGCCCCGG 
GACGAACGGC GGGTCGTGGG CACGGACAGC GTCGTCCACA TGCTCCGGTC GGCGCCCGAC 
CCCGCCGAGG CCCTGGAACT CTTCACGGCC GCGGCGCGGC AGCCCACGAA GGTCCACACA 
ACGGAGTCGT GCAACTACAT GCTGGAGCTG ATGCGCGCCC ACGGTCGTGT CGGGGACATG 
GCGCAGGTGT TCGACCTAAT GCAGAAACAG GTCGTCAAGA CCAACGTCGG CACCTTCGCC 
ACCATCTTCG GCGGCGTCGG CGTCGAGGGG GGGCTCCGCA GCGCGCCGGT CGCCCTGCCT 
GTGATGAGGG AGGCGGGGAT GTCCCTCAAC GCCTACACGT ACAATGGCTT GATTTACTTC 
CTCGTTAAGT CCGGCTTCGA TGCGGAGGCC ATGGAGGTTT ACAAGGCGAT GGTTGAGGAC 
GGCATCTCGC CGAGTGTGAG GACCTACTCC GTGCTGATGG TGGCGTTTGG GAAGAAGAGG 
GATGTCGACA CTGTTCTCTG GTTGCTGAAC GAGATGGAGG CCCGCGGCGT CAAGCCAAAC 
GTGTATAGCT ACACCATCTG CATTCGGGTT CTTGGGCAGG CCGCAAGGTT TGATGAAGCA 
TATCACATTC TTGGAAAGAT GGAGGATTCA GGGTGCAAAC CAGATGTCGT CACACATACT 
GTGATTATAC AGGTTCTCTG TGATGCTGGC CGTCTGAGCG ACGCCAAGGC TGTCTTTTGG 
AAGATGAAAG CGAGTGATCA GAAACCTGAT CGTGTCACTT ACATCACTCT CTTAGACAAG 
TGTGGTGACA GTGGTGACTC ACAATCAGTT GTCGAAGTTT GGAATGCCAT GGTAGCTGAT 
GGTTATAATG ACAATATTGT TTCTTACACT GCAGTTGTGG ATGCATTATG CCAAGTTGGC 
CGGGTTGATG AAGCTTTAGC TGTGTTTGAT GAGATGAAGG AAAAGGGTAT GTCGCCTGAG 
CAGTATTCCT ATAACTCCTT AATATCTGGG TTTCTGAAAG CTGATATGTT TGACCGTGCT 
CTGGAGCTCT TCAACCATAT GAATGCTTGT GGTCCTAGTC CAAATGGCTA CACTCATGTC 
CTCTTCATCA ACTACTACGG AAAATCTGGT CAATCTTTGA AAGCAATACA AAGATATGAG 
CACATGAAGA GCAAAGGGAT TGTTCCTGAT GTTGCTGCTG CTAATGCTGT TCTGTATAGT 
CTTGCTGGAT CTGGCAGACT TGGAATGGCA AAAAGGGTCT TTTATGAACT AAAAGCGATG 
GGAGTCTCTC CAGACACTAT CACGTACACA ATGATGATCA AATGTTGCAG TAAGGCATCA 
AAAGCTGATG AAGCTATGAA TTTTTTTTCT GACATGGTGG AAAGTGGATG TGTTCCTGAT 
GTTCTTGCAT TGAATTCATT GATTGATACA CTCTACAAGG GAGGCAAGGG AAACGAAGCA 
TGGCAACTTT TCCATAAATT GAAAGAAATG AAAATTGAGC CCACTAATGG TACGTACAAC 
ACACTTTTGT CAGGATTAGG AAGAGAAGGC AAAGTTAAGG AGGTAATGCA GCTGCTCGAA 
GAAATGACAC GTACCATTTA TCCACCTAAT TTAATTACAT ACAACACAGT TCTAGATTGC 
CTCAGCAAAA ATGGGGAGGT GAACTGTGCA ATAGATATGC TTTACAGTAT GACTGAGAAA 
GGTTGCGCAC CTGACCTTTC ATCTTATAAC ACTGTCATGT ATGGCCTTAT TAAAGAGGAA 
AGACTTGAAG AGGCATTCAG GATGTTTTGT CAAATGAAAA AGATTCTTGC TCCAGATTAT 
GCCACACTGT GTACTATCCT CCCAAGTTTT GTTAAAAATG GGTTGATGAA GGAAGCTCTG 
CATACTGTAA AGGAATATAT TTTGAAGGCT GGTTGTAACG TGGACAAGTC TTCATTCCAT 
TCACTGATGG AAGGGATACT GAAGAAGGCT GGTGTGGAAA AATCAATCGA GTTTGCTGAA 
AACATAGCAT CTAGGGGAAT TCTGCTGAAT GATTTCTTTT TATGCCCATT GATTAGACAC 
CTCTGCAAGC ACAAGAAAGC TCTTGAAGCG CATCAACTAT TCAATAAGTT TAAAGGTCTT 
GGAGTTTCAC TAAAAACTGG TTCTTATAAT TCTCTTATCC GTGGCCTTGT GGATGAAAAC 
CTGATTGATA TCGCTGAAGA CTTGTTCACT GAAATGAAAA GACTAGGATG TGGCCCAGAT 
GAATTCACTT ACAATTTGAT TCTTGATGCC ATGGGAAAAT CGATGCGGGT TGAGGAAATG 
TTAAAAGTCC AAAAGGAGAT GCATCGTAAG GGATATGAAT CAACATATGT TACTTATAAC 
ACTATCATTT CGGGTCTCGT AAAATCTAAA AGATTGGAAC AGGCTATAGA CCTGTACTAC 
AATCTGATGA GTGAAGGCTT CTCCCCAACA CCATGTACAT ATGGTCCTCT TCTTGATGGC 
TTATTAAAAT CTGGAAAGAT GGTGGATGCT GAAAATCTTT TTAATGAGAT GCTAGAGTAT 
GGATGCGAAC CTAATTGCAC CATCTACAAT ATTCTACTGA ATGGACACCG AATAGCTGGC 
AACACAGAAA ACGTTTGTCA GCTTTTTGAA AAGATGGTTG AGCAGGGAAT AAACCCAGAT 
ATAAAATCCT ATACAATTCT TATTGATACC CTGTGCACAG CTGGAAGATT AAATGATGGT 
TTGTGTTATT TTAGACAGCT ACATGAATTA GGACTTGAGC CTGATCTTAT CGTTTATAAT 
TTGCTCATTG ATGGTCTTGG AAAATCTGAA AGAATAGAGG AAGCAGTCAG TCTCTTCAAC 
GAGATGAAGA AGAAAGGAAT TATCCCAAAC TTATACACAT ACAATTCACT AATTCTCCAC 
TTAGGGAAAG CAGGGAAAGC TGCTGAAGCT GCGCAAATGT ATGAAGAACT ACTGAGAAAA 
GGATGGAAAC CTAGTGTTTT CACATATAAT GCACTCATCA GGGGTTACAG TGTTTCTGGC 
AGTACCGATA ATGCATACGC TGCCTATGGT CAGATGATTG TTGGAGGGTG CCAGCCTAAT 
TCTAGCACTT ATATGCAACT TCCAAATCAA TTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

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LOC103639062 cDNA ORF clone, Zea mays

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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