RAI1 cDNA ORF clone, Xiphophorus maculatus(Southern platyfish)

Gene
Clones
gRNAs

Summary

Gene Symbol	RAI1
Entrez Gene ID	102216604
Full Name	retinoic acid induced 1
Gene Type	protein-coding
Organism	Xiphophorus maculatus(Southern platyfish)

mRNA and Protein(s)

mRNA	Protein	Name
XM_014472413.2	XP_014327899.2	retinoic acid-induced protein 1 isoform X2
XM_023349067.1	XP_023204835.1	retinoic acid-induced protein 1 isoform X1
XM_014472413.1	XP_014327899.1	retinoic acid-induced protein 1

Pathways from BioSystems
Gene Ontology

The following RAI1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the RAI1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OXi14031	XM_014472413.1 Latest version!	Xiphophorus maculatus retinoic acid induced 1 (rai1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
OXi26246	XM_023349067.1 Latest version!	Xiphophorus maculatus retinoic acid induced 1 (rai1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OXi26247	XM_014472413.2 Latest version!	Xiphophorus maculatus retinoic acid induced 1 (rai1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

RAI1 ( XM_014472413.1 ) cDNA ORF clone, Xiphophorus maculatus(Southern platyfish) -> XP_014327899.1 Xiphophorus maculatus retinoic acid induced 1 (rai1), mRNA.

Price & Availability↑

CloneID	OXi14031
Clone ID Related Accession (Same CDS sequence)	XM_014472413.1
Accession Version	XM_014472413.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2799bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1445702400000
Organism	Xiphophorus maculatus(Southern platyfish)
Product	retinoic acid-induced protein 1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_005372412.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xiphophorus maculatus Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACCGGG AAGATCCAAG ACAACAAGTC CCAGAAATGC AAAGCAGGAC CAGGAAAGCT 
CTAAAACATG CAACACTGCA ATATAACCAA GACGGAAGAT CTCCTACAGA GGAAAAGCGA 
TCTCTCCCAG AAATAAAAGT ACAAGAAAGG GAAAACAACA ATATGGTCTT AAGATCCAGA 
AAAGTCTCAC AGGATAAGCT AACAAAAGAA AAGGAAAGGG GAGGGGAAGA TATTCAAACT 
TCAACACCAA CAGAAAATAA AATCAGCGAT ATCTCTCTAA AAGTTAAAGA GGTCATAGTT 
GTAAAAAAAC TGCTAACTCC AAATCCTGAT GCCATGATAA CAACAAATGT TTCCCTACAC 
TCATCTGTAC CACTTGCAAA GCCAGCCCTC TCTGAAGAAT CTAAAGAAAA GACATTATCA 
ATACTAAAGC GCACATCTAG CCCAGACATT TCCACACCAC CTGTAAAAAA GAAGAGAGGT 
CCAAAGCCCA AACTAAGAAA CTTACCATCT CAGTCAGTCC TGTTTGAAGA GATTCTACCA 
GCACGCAAAG AGAAAAGTGT CCGAGGCCCC AGAAGAAAGC GAGGACCACC CAGGAAAGCT 
TCTCTTGTCA GTGCCTCAAC AAAAGATGCT CCTCCTAACA GCAGTGGATC TACCAACAGT 
GACATGCCTG TGGTGCCTCC TCAGTGCCCC ACCAAAACAA AAGTTCTCCC CCCACGCAAA 
GGCAGAGGAC AGAAATATGA GGCAATGGTA CAGAAGATTA CATCTCCCAG CTCAAAAAAA 
CACTTCCCAG CTACGCAACA AGACAGTAGT CTAAGTGAGG ATTTTACGTC GAAGGCTTTT 
TCTGAATTTG TCTTTAAGGA AGGTGAGGCT TTTATTCAAA TAAATAACAC AGAGACACTA 
AAGTTTACAA AGGAGGAAAT GAAAGAGCAA GAAGAAGCAA TGACAATGGA AGATGTATCA 
CAACAATGTG GCAAGCATGA GTTAAGTCAA GAGGAATCAG CAAAGGATAT AGTGAGACCA 
TTAGCGGAAG AAGATGTTAC AAATAAGAAT TATGGAAGAC AAGAAAAAGG TAAAGTAGAA 
ACCAAGATAG AGGTTGCTGA CAATAAGGCT TCGTCTTCAA CAGAGACATC ATTAGAAGTT 
GGAACTCTAG ACGAGAGGAC ACAACAGACT TCCAAGAGTG ATGCCACAAA GTCTGTCCGA 
ACGAAGAGAA AGAGATGGGC CATGGTGGAA AGTACAGATG CATCAGTGGT AGCTTTGGAA 
GCAGACAGCC TCATTATTAC AACACCAAGA CTTGCTAAAC AGAGAGCTAT TAAGAACAAC 
CATGAAATGC ACCTTAAACA GAGGAGGAAG AAGAGAAAGG GCAAAGCCAA TTTAGAGGAA 
ACTGAGACAC TAGATGAGCT CAACACAACA GAAACGCCAG AACCTGTGGA AAGAATAGAG 
GAAAAAGTCA CCACCACAAA GTCCACAGAA CCTCTACCAA CTAACGGGGA TAAATTCACA 
GAAACCCCCC AAGCCATCTC TGTAGAACTT ATTCAAAAAC CTCGAAGAGG CCGAAAACCA 
TCCGCAAACC CAACTAAACA AAGAAGAAGC CAAGCCTCAA TGGAGCATGC TCCTGGGAAG 
CCAGTGAAGA TCCACAAAAA ACCTGGGCCA AAACCTGGAA TGAAAGATGC CATTGAGGTA 
ATTGAGGCAG TTGTAAGGGC AGCCGGATGT GAGCAGGACC AAAAAGAAGA AAGAGAAAAA 
AGAGAACGAG AGAATATAGA AAGTGAAGAG ACTAGTATTG TGGGTCCTGT GGTGACCGTT 
ACAGAAAAGC AGATGGAGTT CATTTCTGTG AAAAGAATCA GAAGGAGACC AATCAACAGG 
AATTCCAAAC TATCTTTTTG TCCTTATGTA CGTATCAATA ACTCTAGAGA CTTTTCTTCT 
TGGTGTGCCA TAGTCAACAA GCCTGAAGAC GCTGTTGTAT TTCAGAGACG GAGAAAAAAG 
GGCATACTTA GAATGAGGAA TCCTTTCACA GTTGCTAAAG TAGTGCCACA TACTGCTGCC 
ATGCAACTAG GACCATTGGT AAACACAAAT CTATCTGAAA TGCGTCTTAT GTGTTGCTTG 
TGTGGGAAAC CACCAAACTA CAAAGACCTG GGTGATTTGT GTGGACCATA CTACACAGAA 
GATAGTATTC CACGGAAAAT GTTGACTATC ACACATACGC AACTCTTCAG GGAAGATGAC 
AGCAAGACCC ATGAGAATGA CAGTAACAGC AGTGAAGAAC CAGGGACGTC CTCAAAGACT 
GAAGATAGAG TGAACTCAGA AAAGGAGGGA AATACAGAAG CTACCACTGA AGAGGCTGGA 
AACAGCAGGC ACCATCACCG GCACTACAGA CGGCTACTGA GACCAGAAAG ACCGTGTCAG 
GAAGGTGGTC CGAGAAGAGT TACCCTCAGA CAGAGATTCA GGAGAATGAA GCAGCTCCAG 
AATCTCTGCA CGGCCACTTC AAAAGACCAA GAGGGCAATG AAAGCATGTT CCAAAAGCTA 
CAAATGGAGG CAGTGGCCAA GGAGCACTGG GCTCATGAAA ACTGTACCAT CTGGACCAAG 
GGGATAATTA TGGTAGCTGG AAGGCTGTAT GGACTGAAGG AAGCTGCCAA CAAGTCAGTT 
CAAACGAGCT GTCACAAGTG CCAGATTTCG GGAGCGTCTC TCAGCTGCTG TTGGAGAGGC 
TGCTCTTATA AGTACCACTA CATCTGCGCC AAAGAGATAG GCTGCACATT TCATGAGGAT 
GATTTTTCCA TCAAATGCCC AAAACATGAG GATCTGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_014327899.1
CDS	86..2884
Translation	MDREDPRQQVPEMQSRTRKALKHATLQYNQDGRSPTEEKRSLPEIKVQERENNNMVLRSRKVSQDKLTKEKERGGEDIQTSTPTENKISDISLKVKEVIVVKKLLTPNPDAMITTNVSLHSSVPLAKPALSEESKEKTLSILKRTSSPDISTPPVKKKRGPKPKLRNLPSQSVLFEEILPARKEKSVRGPRRKRGPPRKASLVSASTKDAPPNSSGSTNSDMPVVPPQCPTKTKVLPPRKGRGQKYEAMVQKITSPSSKKHFPATQQDSSLSEDFTSKAFSEFVFKEGEAFIQINNTETLKFTKEEMKEQEEAMTMEDVSQQCGKHELSQEESAKDIVRPLAEEDVTNKNYGRQEKGKVETKIEVADNKASSSTETSLEVGTLDERTQQTSKSDATKSVRTKRKRWAMVESTDASVVALEADSLIITTPRLAKQRAIKNNHEMHLKQRRKKRKGKANLEETETLDELNTTETPEPVERIEEKVTTTKSTEPLPTNGDKFTETPQAISVELIQKPRRGRKPSANPTKQRRSQASMEHAPGKPVKIHKKPGPKPGMKDAIEVIEAVVRAAGCEQDQKEEREKRERENIESEETSIVGPVVTVTEKQMEFISVKRIRRRPINRNSKLSFCPYVRINNSRDFSSWCAIVNKPEDAVVFQRRRKKGILRMRNPFTVAKVVPHTAAMQLGPLVNTNLSEMRLMCCLCGKPPNYKDLGDLCGPYYTEDSIPRKMLTITHTQLFREDDSKTHENDSNSSEEPGTSSKTEDRVNSEKEGNTEATTEEAGNSRHHHRHYRRLLRPERPCQEGGPRRVTLRQRFRRMKQLQNLCTATSKDQEGNESMFQKLQMEAVAKEHWAHENCTIWTKGIIMVAGRLYGLKEAANKSVQTSCHKCQISGASLSCCWRGCSYKYHYICAKEIGCTFHEDDFSIKCPKHEDL

Target ORF information:

RefSeq Version	XM_014472413.1
Organism	Xiphophorus maculatus(Southern platyfish)
Definition	Xiphophorus maculatus retinoic acid induced 1 (rai1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014472413.1

ORF Insert Sequence:

ATGGACCGGG AAGATCCAAG ACAACAAGTC CCAGAAATGC AAAGCAGGAC CAGGAAAGCT 
CTAAAACATG CAACACTGCA ATATAACCAA GACGGAAGAT CTCCTACAGA GGAAAAGCGA 
TCTCTCCCAG AAATAAAAGT ACAAGAAAGG GAAAACAACA ATATGGTCTT AAGATCCAGA 
AAAGTCTCAC AGGATAAGCT AACAAAAGAA AAGGAAAGGG GAGGGGAAGA TATTCAAACT 
TCAACACCAA CAGAAAATAA AATCAGCGAT ATCTCTCTAA AAGTTAAAGA GGTCATAGTT 
GTAAAAAAAC TGCTAACTCC AAATCCTGAT GCCATGATAA CAACAAATGT TTCCCTACAC 
TCATCTGTAC CACTTGCAAA GCCAGCCCTC TCTGAAGAAT CTAAAGAAAA GACATTATCA 
ATACTAAAGC GCACATCTAG CCCAGACATT TCCACACCAC CTGTAAAAAA GAAGAGAGGT 
CCAAAGCCCA AACTAAGAAA CTTACCATCT CAGTCAGTCC TGTTTGAAGA GATTCTACCA 
GCACGCAAAG AGAAAAGTGT CCGAGGCCCC AGAAGAAAGC GAGGACCACC CAGGAAAGCT 
TCTCTTGTCA GTGCCTCAAC AAAAGATGCT CCTCCTAACA GCAGTGGATC TACCAACAGT 
GACATGCCTG TGGTGCCTCC TCAGTGCCCC ACCAAAACAA AAGTTCTCCC CCCACGCAAA 
GGCAGAGGAC AGAAATATGA GGCAATGGTA CAGAAGATTA CATCTCCCAG CTCAAAAAAA 
CACTTCCCAG CTACGCAACA AGACAGTAGT CTAAGTGAGG ATTTTACGTC GAAGGCTTTT 
TCTGAATTTG TCTTTAAGGA AGGTGAGGCT TTTATTCAAA TAAATAACAC AGAGACACTA 
AAGTTTACAA AGGAGGAAAT GAAAGAGCAA GAAGAAGCAA TGACAATGGA AGATGTATCA 
CAACAATGTG GCAAGCATGA GTTAAGTCAA GAGGAATCAG CAAAGGATAT AGTGAGACCA 
TTAGCGGAAG AAGATGTTAC AAATAAGAAT TATGGAAGAC AAGAAAAAGG TAAAGTAGAA 
ACCAAGATAG AGGTTGCTGA CAATAAGGCT TCGTCTTCAA CAGAGACATC ATTAGAAGTT 
GGAACTCTAG ACGAGAGGAC ACAACAGACT TCCAAGAGTG ATGCCACAAA GTCTGTCCGA 
ACGAAGAGAA AGAGATGGGC CATGGTGGAA AGTACAGATG CATCAGTGGT AGCTTTGGAA 
GCAGACAGCC TCATTATTAC AACACCAAGA CTTGCTAAAC AGAGAGCTAT TAAGAACAAC 
CATGAAATGC ACCTTAAACA GAGGAGGAAG AAGAGAAAGG GCAAAGCCAA TTTAGAGGAA 
ACTGAGACAC TAGATGAGCT CAACACAACA GAAACGCCAG AACCTGTGGA AAGAATAGAG 
GAAAAAGTCA CCACCACAAA GTCCACAGAA CCTCTACCAA CTAACGGGGA TAAATTCACA 
GAAACCCCCC AAGCCATCTC TGTAGAACTT ATTCAAAAAC CTCGAAGAGG CCGAAAACCA 
TCCGCAAACC CAACTAAACA AAGAAGAAGC CAAGCCTCAA TGGAGCATGC TCCTGGGAAG 
CCAGTGAAGA TCCACAAAAA ACCTGGGCCA AAACCTGGAA TGAAAGATGC CATTGAGGTA 
ATTGAGGCAG TTGTAAGGGC AGCCGGATGT GAGCAGGACC AAAAAGAAGA AAGAGAAAAA 
AGAGAACGAG AGAATATAGA AAGTGAAGAG ACTAGTATTG TGGGTCCTGT GGTGACCGTT 
ACAGAAAAGC AGATGGAGTT CATTTCTGTG AAAAGAATCA GAAGGAGACC AATCAACAGG 
AATTCCAAAC TATCTTTTTG TCCTTATGTA CGTATCAATA ACTCTAGAGA CTTTTCTTCT 
TGGTGTGCCA TAGTCAACAA GCCTGAAGAC GCTGTTGTAT TTCAGAGACG GAGAAAAAAG 
GGCATACTTA GAATGAGGAA TCCTTTCACA GTTGCTAAAG TAGTGCCACA TACTGCTGCC 
ATGCAACTAG GACCATTGGT AAACACAAAT CTATCTGAAA TGCGTCTTAT GTGTTGCTTG 
TGTGGGAAAC CACCAAACTA CAAAGACCTG GGTGATTTGT GTGGACCATA CTACACAGAA 
GATAGTATTC CACGGAAAAT GTTGACTATC ACACATACGC AACTCTTCAG GGAAGATGAC 
AGCAAGACCC ATGAGAATGA CAGTAACAGC AGTGAAGAAC CAGGGACGTC CTCAAAGACT 
GAAGATAGAG TGAACTCAGA AAAGGAGGGA AATACAGAAG CTACCACTGA AGAGGCTGGA 
AACAGCAGGC ACCATCACCG GCACTACAGA CGGCTACTGA GACCAGAAAG ACCGTGTCAG 
GAAGGTGGTC CGAGAAGAGT TACCCTCAGA CAGAGATTCA GGAGAATGAA GCAGCTCCAG 
AATCTCTGCA CGGCCACTTC AAAAGACCAA GAGGGCAATG AAAGCATGTT CCAAAAGCTA 
CAAATGGAGG CAGTGGCCAA GGAGCACTGG GCTCATGAAA ACTGTACCAT CTGGACCAAG 
GGGATAATTA TGGTAGCTGG AAGGCTGTAT GGACTGAAGG AAGCTGCCAA CAAGTCAGTT 
CAAACGAGCT GTCACAAGTG CCAGATTTCG GGAGCGTCTC TCAGCTGCTG TTGGAGAGGC 
TGCTCTTATA AGTACCACTA CATCTGCGCC AAAGAGATAG GCTGCACATT TCATGAGGAT 
GATTTTTCCA TCAAATGCCC AAAACATGAG GATCTGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RAI1 ( XM_023349067.1 ) cDNA ORF clone, Xiphophorus maculatus(Southern platyfish) -> XP_023204835.1 Xiphophorus maculatus retinoic acid induced 1 (rai1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OXi26246
Clone ID Related Accession (Same CDS sequence)	XM_023349067.1
Accession Version	XM_023349067.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6594bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1513785600000
Organism	Xiphophorus maculatus(southern platyfish)
Product	retinoic acid-induced protein 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_036458.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xiphophorus maculatus Annotation Release 102 Annotation Version :: 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAGTCCT TTCGTGAGCG CAACAGTGGT TACCACAGCA ACCAACCCTG CTATCAGCAG 
GAGCCCCATG AATTATCTCG CCTGGAGACC TACCGGCAAC ACCCACATCA TCCCCATCCG 
CAGCATCCAC ACCCAGGCCC TGGTCCACAT CCAGGCCCTG GCCCTGGGCA CAACAGGTCA 
GGCTATGAGG CTATGTCTAA TGCAGCAAAC GTGCCCACAG GTGGAGCAGC TGGAATTGGA 
GGACCCAAGG ACTGTTACAG CCAACAAAAC TATTCTGGGT ACCCAGGAAG TACTGGAGGC 
AGTGGAAATG GAAATGGTAG CTCATCAGCC TCACAGGCAA AAAAATCCTT TAGAGGAAGC 
AAAGTTCCCC CACCAAACCC CAGTCAACAC CTGCAGGGCC CTGGAGGCTA TAGTAATCAC 
ATGGGCCCAG GAAATTATTC TGCCCAGTAC ATGAGCGAGG GCCATCTCCA GCAGAAATGG 
GAGGACCCTG CACAGTTAGC ACAGTATGAA CAGGAGATGG TGGGGCGACT GGAGGCTGGT 
GCTTCCCCTG CACCTGGATC CTCCCAGTAC ATAGACTCAA ACATGTTGGG GCACTCCCAA 
ACCCCATGCC ACCAGCCTTC TACCCCAAGT TATACAAGTC CACATCACCA GTCTCACCCT 
CCTAATCCTG CTCCCTCACC TCTCATGTAT CCCCAAAGTC ACCTACACTA TCCTCAACCC 
TCCCCCTCTC CATCCCCATA CATGGAAAAA TGCAGTCCCA TGCCCCATTG TTATAAAGGC 
TACAACATAC CTCCAAATTC TCAGTATAGC AGACAGCTAA GTAGTCATAG CAGTTTGAAG 
CAGGGGACTT ACAGGTCAAC CCAGAACAGC TATGGATACC AGCAGTCAAC ACCCAGAGGT 
TATGAGCAGC AGACATCTTT ACAGGCAATG ACCAACCCCC AAGAACCCCA CCCTAAATAC 
CAACACTATA GCCAACCCCA ACAAAATTAC TGTCTGTCAG AGCTCCCTGT CAGATCTCCA 
GAACAGTATT ATCAGACTTG CAGCCCCTCC TCAAGTCATT CCCCTGCTCG CTCTGTCGGA 
CGCTCACCCT CATACAGTTC CACCCCTTCA CCACTGATGA CTAATCCAGA ATCATTCCAG 
TATGGCCAAC CACCAATGAC ACCTGGGGCA GCCTCGTCGT CGTCATCCTC TTCAGCTGGT 
ATGCAAGATC AAGCCAGCAC AAACACCATG CTAATGCCCC CACGGTCCCA CCCTTCACCC 
AACATATCCC ACACAGCATC CCACAGCTAC ACTTCAACGC CACAAGCGCC CACAATGAAA 
GAGCGCTTCT CAGAGAAGCT GTTATCAAAC CCCAGCTTGT GGAGCCTAAA TGCTCTCACC 
TCTCAGGTGG AGAATATCTC CAATAACGTT CAACAGCTCC TGCTTTCAGA AGCCCTGGTT 
GCAAACAAGA AGGGCAGCAA GCGCAGCAGT GGAGGGAGCA ACTGCAGCAC TGGAAGTGGG 
GCCTCCACCA AGAAAGGTGA AGAATACAAA AGCCCTCCAT ATCCAGACAG TGGTGGCAAT 
GTGGGTGGGG CCCCCATGCA TGAACCTTAC TCTACCCCAC AGCACCAACC ATTGCCTATG 
GAACTCCATG AGGGTGGCTA CTCCAGCAGT AGTGATGAAC CATTGGACAG AGGTTTCTAT 
TACTTTGGCC AGGGTAGAAG TCCAGCCCAG GCCCACAACA ACACACAACT CAGCCTGGAC 
ACAGCCTCCT CTTGCTCCAT TGCCTCTCCA GATGATATGT CTACTAGGTC TGGGGACTCG 
GGGTTGCACA ACCTTACCCC TGATCCTATA AGATGCACGG CAGGGCAAGG AGGAGATGGC 
ATGAATACCC CAGTGAAGAG CATTGGTGAT GAGAGATCTC CAACAAGCAT TACAATACCA 
AGTCCTATGA AACAAGAAAG TGATTCCCCA TCAGACACAC AACACATCAA TCAGCCTGTA 
AAAGAGAACT TTGAAGAGTC AGCTTGGACT GAAAAACCTG CTGGAAAAGA AGAGGAAGCA 
ATGGAAAAAA CTCTTGATTT TATAAGTAAT TCAGACTCAA GCAACCCTAC AGAGAAGCAA 
GGGAAATGGA CTGATGAGGA AAAATGCCCA AATTTCTACA GCAAAATAAA CACGAACATG 
GCAGAAAAAG GCTATTGTTA TGAAGACACA ATGTACCAAG AGGTCCAGAG CAAATATGAC 
TCAGATGCAA GAGATTCCGT TGAACAGTCC CCAGCTGATC TCTCTGATTC CAGTAACAAA 
GATCACTTTA GTCAAGAGAT GAAGTCAGGG ATATTCAAAT CTGAATCTCC AACTGCATCT 
GAGAGCTCTG TGAAAACACT GCCATTCATT TCAAGAGGAG ACCTTGAGCA GGATCCATAT 
TCCACAGAGA AAGATGACAG TTCAGCAAAT ACCTCTCCAA CCCCACAAAC TGAAGTCCTG 
GAAGCTTCTA GCTGTGACAA GAAGGAGAGC AGAACTCAGG AGAATGACAA AGACGTAGTA 
GGAGATAGGG AAGAGGATGA GATAAATGAA GATGAGAAAG AAGAAATTGA AAAAAGTTCT 
CTTTCACAGC TTTTGTCTAA AGAAGTTAGG GAGGAAGCAG AGGAGGAGGA GAAAGAAAGT 
CAGTCATTAA CTGAAGAGCA CACAGATGAC AAAGACATCC AAATGAGTCC CTCTGAAGAT 
CTCTGCAGCA GAACAGAAAT CCACAATTTA GCTCTTAAAA CTGACAATGA TTTTGCAGGG 
GGACCCGGTC ATCTTAATGC AGCAGCAGCA ACGACAGCAA CAGATGTTTC AACAAGGGAG 
TCAGCCATTG GCGACACAGC ACCTCAGCCA CAGTCCGCTA TGCCAGTCTT CTCTGCTCTC 
AATGATAAGG CAGCTCCTCC TGCTCAGGCT AGAGATCATA TTGATCACAG CGATGCTAAG 
GTTCTGGAGC CAGACTCTCC TCAGCTGCCA GGGAAGTCAA TACTTCCCTC TGCCCCTTCC 
TGGGCTGACA CTCCACCATC TCCTCAAAAA GGGGATGATG ATATAGAACC AGGCATAAGT 
TGTTCCAGTG CTGTGACACC ACTAGCTAAA CCAGAGCCTA TGGTTCCATC TGCTCAGCCA 
AGGACATATG GGCGTAAGCA TGTTAGGGGT AGAAGGAGAA TCATACATTC AGGTGTGGCA 
TTAAGGCGAC AACTGAGCTT GAAGAAGGAG GAAGAAAAGG AGGACAAAGA AACAAATTCC 
TCTCAGCAAA AATCGAGCAT GGCTCCAAGC AAGACTGTTT TATTCTCAGA TCAAACTAAC 
CTTGCTCATC AGGATTCTAT GGTGAGCCAG ACTCCTAAAA TGCTGACAGA TGGTTTTCAA 
TCCAGAATGT GCACTCGCTC TTTTAATGCA CCAGATATGC AAACAAAAGT TGAACCTAAT 
GTGAAGAGAA AACCAGGCCC AAAACCTGGA TCTAAGCCAG GGTCAAAACC CAGTGCAAAA 
CCAGGGCCTA AGCCAGGTGC TAAGCCTGGT CTAAAGTCAG GTCCCAAACC TGGACCAAAA 
CCTGGGAAAA AGACAGGACA AAAGCCTGCA TCACGTGAAT CAGAAGTGCC ACTGAAAATT 
GATACTGTTG TAAGGCAAAA ACCAGGGCCA AAACCAGGTT CAAAATCTGG ACCAAAACCA 
GCCCTAAGAC CAGGTCTAAA ACCTGGATTC AAGCCTGCTG AGGTTTTGCC TCCTACTGAG 
GCTGCTCCCA TCAAAGCTCC AGTTGGTCGC CCCAGGGGCT CAATTTCAAA ATCAAAATTG 
ATGGACCGGG AAGATCCAAG ACAACAAGTC CCAGAAATGC AAAGCAGGAC CAGGAAAGCT 
CTAAAACATG CAACACTGCA ATATAACCAA GACGGAAGAT CTCCTACAGA GGAAAAGCGA 
TCTCTCCCAG AAATAAAAGT ACAAGAAAGG GAAAACAACA ATATGGTCTT AAGATCCAGA 
AAAGTCTCAC AGGATAAGCT AACAAAAGAA AAGGAAAGGG GAGGGGAAGA TATTCAAACT 
TCAACACCAA CAGAAAATAA AATCAGCGAT ATCTCTCTAA AAGTTAAAGA GGTCATAGTT 
GTAAAAAAAC TGCTAACTCC AAATCCTGAT GCCATGATAA CAACAAATGT TTCCCTACAC 
TCATCTGTAC CACTTGCAAA GCCAGCCCTC TCTGAAGAAT CTAAAGAAAA GACATTATCA 
ATACTAAAGC GCACATCTAG CCCAGACATT TCCACACCAC CTGTAAAAAA GAAGAGAGGT 
CCAAAGCCCA AACTAAGAAA CTTACCATCT CAGTCAGTCC TGTTTGAAGA GATTCTACCA 
GCACGCAAAG AGAAAAGTGT CCGAGGCCCC AGAAGAAAGC GAGGACCACC CAGGAAAGCT 
TCTCTTGTCA GTGCCTCAAC AAAAGATGCT CCTCCTAACA GCAGTGGATC TACCAACAGT 
GACATGCCTG TGGTGCCTCC TCAGTGCCCC ACCAAAACAA AAGTTCTCCC CCCACGCAAA 
GGCAGAGGAC AGAAATATGA GGCAATGGTA CAGAAGATTA CATCTCCCAG CTCAAAAAAA 
CACTTCCCAG CTACGCAACA AGACAGTAGT CTAAGTGAGG ATTTTACGTC GAAGGCTTTT 
TCTGAATTTG TCTTTAAGGA AGGTGAGGCT TTTATTCAAA TAAATAACAC AGAGACACTA 
AAGTTTACAA AGGAGGAAAT GAAAGAGCAA GAAGAAGCAA TGACAATGGA AGATGTATCA 
CAACAATGTG GCAAGCATGA GTTAAGTCAA GAGGAATCAG CAAAGGATAT AGTGAGACCA 
TTAGCGGAAG AAGATGTTAC AAATAAGAAT TATGGAAGAC AAGAAAAAGG TAAAGTAGAA 
ACCAAGATAG AGGTTGCTGA CAATAAGGCT TCGTCTTCAA CAGAGACATC ATTAGAAGTT 
GGAACTCTAG ACGAGAGGAC ACAACAGACT TCCAAGAGTG ATGCCACAAA GTCTGTCCGA 
ACGAAGAGAA AGAGATGGGC CATGGTGGAA AGTACAGATG CATCAGTGGT AGCTTTGGAA 
GCAGACAGCC TCATTATTAC AACACCAAGA CTTGCTAAAC AGAGAGCTAT TAAGAACAAC 
CATGAAATGC ACCTTAAACA GAGGAGGAAG AAGAGAAAGG GCAAAGCCAA TTTAGAGGAA 
ACTGAGACAC TAGATGAGCT CAACACAACA GAAACGCCAG AACCTGTGGA AAGAATAGAG 
GAAAAAGTCA CCACCACAAA GTCCACAGAA CCTCTACCAA CTAACGGGGA TAAATTCACA 
GAAACCCCCC AAGCCATCTC TGTAGAACTT ATTCAAAAAC CTCGAAGAGG CCGAAAACCA 
TCCGCAAACC CAACTAAACA AAGAAGAAGC CAAGCCTCAA TGGAGCATGC TCCTGGGAAG 
CCAGTGAAGA TCCACAAAAA ACCTGGGCCA AAACCTGGAA TGAAAGATGC CATTGAGGTA 
ATTGAGGCAG TTGTAAGGGC AGCCGGATGT GAGCAGGACC AAAAAGAAGA AAGAGAAAAA 
AGAGAACGAG AGAATATAGA AAGTGAAGAG ACTAGTATTG TGGGTCCTGT GGTGACCGTT 
ACAGAAAAGC AGATGGAGTT CATTTCTGTG AAAAGAATCA GAAGGAGACC AATCAACAGG 
AATTCCAAAC TATCTTTTTG TCCTTATGTA CGTATCAATA ACTCTAGAGA CTTTTCTTCT 
TGGTGTGCCA TAGTCAACAA GCCTGAAGAC GCTGTTGTAT TTCAGAGACG GAGAAAAAAG 
GGCATACTTA GAATGAGGAA TCCTTTCACA GTTGCTAAAG TAGTGCCACA TACTGCTGCC 
ATGCAACTAG GACCATTGGT AAACACAAAT CTATCTGAAA TGCGTCTTAT GTGTTGCTTG 
TGTGGGAAAC CACCAAACTA CAAAGACCTG GGTGATTTGT GTGGACCATA CTACACAGAA 
GATAGTATTC CACGGAAAAT GTTGACTATC ACACATACGC AACTCTTCAG GGAAGATGAC 
AGCAAGACCC ATGAGAATGA CAGTAACAGC AGTGAAGAAC CAGGGACGTC CTCAAAGACT 
GAAGATAGAG TGAACTCAGA AAAGGAGGGA AATACAGAAG CTACCACTGA AGAGGCTGGA 
AACAGCAGGC ACCATCACCG GCACTACAGA CGGCTACTGA GACCAGAAAG ACCGTGTCAG 
GAAGGTGGTC CGAGAAGAGT TACCCTCAGA CAGAGATTCA GGAGAATGAA GCAGCTCCAG 
AATCTCTGCA CGGCCACTTC AAAAGACCAA GAGGGCAATG AAAGCATGTT CCAAAAGCTA 
CAAATGGAGG CAGTGGCCAA GGAGCACTGG GCTCATGAAA ACTGTACCAT CTGGACCAAG 
GGGATAATTA TGGTAGCTGG AAGGCTGTAT GGACTGAAGG AAGCTGCCAA CAAGTCAGTT 
CAAACGAGCT GTCACAAGTG CCAGATTTCG GGAGCGTCTC TCAGCTGCTG TTGGAGAGGC 
TGCTCTTATA AGTACCACTA CATCTGCGCC AAAGAGATAG GCTGCACATT TCATGAGGAT 
GATTTTTCCA TCAAATGCCC AAAACATGAG GTAAGACAAG AAAACTGTTA CTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_023204835.1
CDS	747..7340
Translation	MQSFRERNSGYHSNQPCYQQEPHELSRLETYRQHPHHPHPQHPHPGPGPHPGPGPGHNRSGYEAMSNAANVPTGGAAGIGGPKDCYSQQNYSGYPGSTGGSGNGNGSSSASQAKKSFRGSKVPPPNPSQHLQGPGGYSNHMGPGNYSAQYMSEGHLQQKWEDPAQLAQYEQEMVGRLEAGASPAPGSSQYIDSNMLGHSQTPCHQPSTPSYTSPHHQSHPPNPAPSPLMYPQSHLHYPQPSPSPSPYMEKCSPMPHCYKGYNIPPNSQYSRQLSSHSSLKQGTYRSTQNSYGYQQSTPRGYEQQTSLQAMTNPQEPHPKYQHYSQPQQNYCLSELPVRSPEQYYQTCSPSSSHSPARSVGRSPSYSSTPSPLMTNPESFQYGQPPMTPGAASSSSSSSAGMQDQASTNTMLMPPRSHPSPNISHTASHSYTSTPQAPTMKERFSEKLLSNPSLWSLNALTSQVENISNNVQQLLLSEALVANKKGSKRSSGGSNCSTGSGASTKKGEEYKSPPYPDSGGNVGGAPMHEPYSTPQHQPLPMELHEGGYSSSSDEPLDRGFYYFGQGRSPAQAHNNTQLSLDTASSCSIASPDDMSTRSGDSGLHNLTPDPIRCTAGQGGDGMNTPVKSIGDERSPTSITIPSPMKQESDSPSDTQHINQPVKENFEESAWTEKPAGKEEEAMEKTLDFISNSDSSNPTEKQGKWTDEEKCPNFYSKINTNMAEKGYCYEDTMYQEVQSKYDSDARDSVEQSPADLSDSSNKDHFSQEMKSGIFKSESPTASESSVKTLPFISRGDLEQDPYSTEKDDSSANTSPTPQTEVLEASSCDKKESRTQENDKDVVGDREEDEINEDEKEEIEKSSLSQLLSKEVREEAEEEEKESQSLTEEHTDDKDIQMSPSEDLCSRTEIHNLALKTDNDFAGGPGHLNAAAATTATDVSTRESAIGDTAPQPQSAMPVFSALNDKAAPPAQARDHIDHSDAKVLEPDSPQLPGKSILPSAPSWADTPPSPQKGDDDIEPGISCSSAVTPLAKPEPMVPSAQPRTYGRKHVRGRRRIIHSGVALRRQLSLKKEEEKEDKETNSSQQKSSMAPSKTVLFSDQTNLAHQDSMVSQTPKMLTDGFQSRMCTRSFNAPDMQTKVEPNVKRKPGPKPGSKPGSKPSAKPGPKPGAKPGLKSGPKPGPKPGKKTGQKPASRESEVPLKIDTVVRQKPGPKPGSKSGPKPALRPGLKPGFKPAEVLPPTEAAPIKAPVGRPRGSISKSKLMDREDPRQQVPEMQSRTRKALKHATLQYNQDGRSPTEEKRSLPEIKVQERENNNMVLRSRKVSQDKLTKEKERGGEDIQTSTPTENKISDISLKVKEVIVVKKLLTPNPDAMITTNVSLHSSVPLAKPALSEESKEKTLSILKRTSSPDISTPPVKKKRGPKPKLRNLPSQSVLFEEILPARKEKSVRGPRRKRGPPRKASLVSASTKDAPPNSSGSTNSDMPVVPPQCPTKTKVLPPRKGRGQKYEAMVQKITSPSSKKHFPATQQDSSLSEDFTSKAFSEFVFKEGEAFIQINNTETLKFTKEEMKEQEEAMTMEDVSQQCGKHELSQEESAKDIVRPLAEEDVTNKNYGRQEKGKVETKIEVADNKASSSTETSLEVGTLDERTQQTSKSDATKSVRTKRKRWAMVESTDASVVALEADSLIITTPRLAKQRAIKNNHEMHLKQRRKKRKGKANLEETETLDELNTTETPEPVERIEEKVTTTKSTEPLPTNGDKFTETPQAISVELIQKPRRGRKPSANPTKQRRSQASMEHAPGKPVKIHKKPGPKPGMKDAIEVIEAVVRAAGCEQDQKEEREKRERENIESEETSIVGPVVTVTEKQMEFISVKRIRRRPINRNSKLSFCPYVRINNSRDFSSWCAIVNKPEDAVVFQRRRKKGILRMRNPFTVAKVVPHTAAMQLGPLVNTNLSEMRLMCCLCGKPPNYKDLGDLCGPYYTEDSIPRKMLTITHTQLFREDDSKTHENDSNSSEEPGTSSKTEDRVNSEKEGNTEATTEEAGNSRHHHRHYRRLLRPERPCQEGGPRRVTLRQRFRRMKQLQNLCTATSKDQEGNESMFQKLQMEAVAKEHWAHENCTIWTKGIIMVAGRLYGLKEAANKSVQTSCHKCQISGASLSCCWRGCSYKYHYICAKEIGCTFHEDDFSIKCPKHEVRQENCY

Target ORF information:

RefSeq Version	XM_023349067.1
Organism	Xiphophorus maculatus(southern platyfish)
Definition	Xiphophorus maculatus retinoic acid induced 1 (rai1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_023349067.1

ORF Insert Sequence:

ATGCAGTCCT TTCGTGAGCG CAACAGTGGT TACCACAGCA ACCAACCCTG CTATCAGCAG 
GAGCCCCATG AATTATCTCG CCTGGAGACC TACCGGCAAC ACCCACATCA TCCCCATCCG 
CAGCATCCAC ACCCAGGCCC TGGTCCACAT CCAGGCCCTG GCCCTGGGCA CAACAGGTCA 
GGCTATGAGG CTATGTCTAA TGCAGCAAAC GTGCCCACAG GTGGAGCAGC TGGAATTGGA 
GGACCCAAGG ACTGTTACAG CCAACAAAAC TATTCTGGGT ACCCAGGAAG TACTGGAGGC 
AGTGGAAATG GAAATGGTAG CTCATCAGCC TCACAGGCAA AAAAATCCTT TAGAGGAAGC 
AAAGTTCCCC CACCAAACCC CAGTCAACAC CTGCAGGGCC CTGGAGGCTA TAGTAATCAC 
ATGGGCCCAG GAAATTATTC TGCCCAGTAC ATGAGCGAGG GCCATCTCCA GCAGAAATGG 
GAGGACCCTG CACAGTTAGC ACAGTATGAA CAGGAGATGG TGGGGCGACT GGAGGCTGGT 
GCTTCCCCTG CACCTGGATC CTCCCAGTAC ATAGACTCAA ACATGTTGGG GCACTCCCAA 
ACCCCATGCC ACCAGCCTTC TACCCCAAGT TATACAAGTC CACATCACCA GTCTCACCCT 
CCTAATCCTG CTCCCTCACC TCTCATGTAT CCCCAAAGTC ACCTACACTA TCCTCAACCC 
TCCCCCTCTC CATCCCCATA CATGGAAAAA TGCAGTCCCA TGCCCCATTG TTATAAAGGC 
TACAACATAC CTCCAAATTC TCAGTATAGC AGACAGCTAA GTAGTCATAG CAGTTTGAAG 
CAGGGGACTT ACAGGTCAAC CCAGAACAGC TATGGATACC AGCAGTCAAC ACCCAGAGGT 
TATGAGCAGC AGACATCTTT ACAGGCAATG ACCAACCCCC AAGAACCCCA CCCTAAATAC 
CAACACTATA GCCAACCCCA ACAAAATTAC TGTCTGTCAG AGCTCCCTGT CAGATCTCCA 
GAACAGTATT ATCAGACTTG CAGCCCCTCC TCAAGTCATT CCCCTGCTCG CTCTGTCGGA 
CGCTCACCCT CATACAGTTC CACCCCTTCA CCACTGATGA CTAATCCAGA ATCATTCCAG 
TATGGCCAAC CACCAATGAC ACCTGGGGCA GCCTCGTCGT CGTCATCCTC TTCAGCTGGT 
ATGCAAGATC AAGCCAGCAC AAACACCATG CTAATGCCCC CACGGTCCCA CCCTTCACCC 
AACATATCCC ACACAGCATC CCACAGCTAC ACTTCAACGC CACAAGCGCC CACAATGAAA 
GAGCGCTTCT CAGAGAAGCT GTTATCAAAC CCCAGCTTGT GGAGCCTAAA TGCTCTCACC 
TCTCAGGTGG AGAATATCTC CAATAACGTT CAACAGCTCC TGCTTTCAGA AGCCCTGGTT 
GCAAACAAGA AGGGCAGCAA GCGCAGCAGT GGAGGGAGCA ACTGCAGCAC TGGAAGTGGG 
GCCTCCACCA AGAAAGGTGA AGAATACAAA AGCCCTCCAT ATCCAGACAG TGGTGGCAAT 
GTGGGTGGGG CCCCCATGCA TGAACCTTAC TCTACCCCAC AGCACCAACC ATTGCCTATG 
GAACTCCATG AGGGTGGCTA CTCCAGCAGT AGTGATGAAC CATTGGACAG AGGTTTCTAT 
TACTTTGGCC AGGGTAGAAG TCCAGCCCAG GCCCACAACA ACACACAACT CAGCCTGGAC 
ACAGCCTCCT CTTGCTCCAT TGCCTCTCCA GATGATATGT CTACTAGGTC TGGGGACTCG 
GGGTTGCACA ACCTTACCCC TGATCCTATA AGATGCACGG CAGGGCAAGG AGGAGATGGC 
ATGAATACCC CAGTGAAGAG CATTGGTGAT GAGAGATCTC CAACAAGCAT TACAATACCA 
AGTCCTATGA AACAAGAAAG TGATTCCCCA TCAGACACAC AACACATCAA TCAGCCTGTA 
AAAGAGAACT TTGAAGAGTC AGCTTGGACT GAAAAACCTG CTGGAAAAGA AGAGGAAGCA 
ATGGAAAAAA CTCTTGATTT TATAAGTAAT TCAGACTCAA GCAACCCTAC AGAGAAGCAA 
GGGAAATGGA CTGATGAGGA AAAATGCCCA AATTTCTACA GCAAAATAAA CACGAACATG 
GCAGAAAAAG GCTATTGTTA TGAAGACACA ATGTACCAAG AGGTCCAGAG CAAATATGAC 
TCAGATGCAA GAGATTCCGT TGAACAGTCC CCAGCTGATC TCTCTGATTC CAGTAACAAA 
GATCACTTTA GTCAAGAGAT GAAGTCAGGG ATATTCAAAT CTGAATCTCC AACTGCATCT 
GAGAGCTCTG TGAAAACACT GCCATTCATT TCAAGAGGAG ACCTTGAGCA GGATCCATAT 
TCCACAGAGA AAGATGACAG TTCAGCAAAT ACCTCTCCAA CCCCACAAAC TGAAGTCCTG 
GAAGCTTCTA GCTGTGACAA GAAGGAGAGC AGAACTCAGG AGAATGACAA AGACGTAGTA 
GGAGATAGGG AAGAGGATGA GATAAATGAA GATGAGAAAG AAGAAATTGA AAAAAGTTCT 
CTTTCACAGC TTTTGTCTAA AGAAGTTAGG GAGGAAGCAG AGGAGGAGGA GAAAGAAAGT 
CAGTCATTAA CTGAAGAGCA CACAGATGAC AAAGACATCC AAATGAGTCC CTCTGAAGAT 
CTCTGCAGCA GAACAGAAAT CCACAATTTA GCTCTTAAAA CTGACAATGA TTTTGCAGGG 
GGACCCGGTC ATCTTAATGC AGCAGCAGCA ACGACAGCAA CAGATGTTTC AACAAGGGAG 
TCAGCCATTG GCGACACAGC ACCTCAGCCA CAGTCCGCTA TGCCAGTCTT CTCTGCTCTC 
AATGATAAGG CAGCTCCTCC TGCTCAGGCT AGAGATCATA TTGATCACAG CGATGCTAAG 
GTTCTGGAGC CAGACTCTCC TCAGCTGCCA GGGAAGTCAA TACTTCCCTC TGCCCCTTCC 
TGGGCTGACA CTCCACCATC TCCTCAAAAA GGGGATGATG ATATAGAACC AGGCATAAGT 
TGTTCCAGTG CTGTGACACC ACTAGCTAAA CCAGAGCCTA TGGTTCCATC TGCTCAGCCA 
AGGACATATG GGCGTAAGCA TGTTAGGGGT AGAAGGAGAA TCATACATTC AGGTGTGGCA 
TTAAGGCGAC AACTGAGCTT GAAGAAGGAG GAAGAAAAGG AGGACAAAGA AACAAATTCC 
TCTCAGCAAA AATCGAGCAT GGCTCCAAGC AAGACTGTTT TATTCTCAGA TCAAACTAAC 
CTTGCTCATC AGGATTCTAT GGTGAGCCAG ACTCCTAAAA TGCTGACAGA TGGTTTTCAA 
TCCAGAATGT GCACTCGCTC TTTTAATGCA CCAGATATGC AAACAAAAGT TGAACCTAAT 
GTGAAGAGAA AACCAGGCCC AAAACCTGGA TCTAAGCCAG GGTCAAAACC CAGTGCAAAA 
CCAGGGCCTA AGCCAGGTGC TAAGCCTGGT CTAAAGTCAG GTCCCAAACC TGGACCAAAA 
CCTGGGAAAA AGACAGGACA AAAGCCTGCA TCACGTGAAT CAGAAGTGCC ACTGAAAATT 
GATACTGTTG TAAGGCAAAA ACCAGGGCCA AAACCAGGTT CAAAATCTGG ACCAAAACCA 
GCCCTAAGAC CAGGTCTAAA ACCTGGATTC AAGCCTGCTG AGGTTTTGCC TCCTACTGAG 
GCTGCTCCCA TCAAAGCTCC AGTTGGTCGC CCCAGGGGCT CAATTTCAAA ATCAAAATTG 
ATGGACCGGG AAGATCCAAG ACAACAAGTC CCAGAAATGC AAAGCAGGAC CAGGAAAGCT 
CTAAAACATG CAACACTGCA ATATAACCAA GACGGAAGAT CTCCTACAGA GGAAAAGCGA 
TCTCTCCCAG AAATAAAAGT ACAAGAAAGG GAAAACAACA ATATGGTCTT AAGATCCAGA 
AAAGTCTCAC AGGATAAGCT AACAAAAGAA AAGGAAAGGG GAGGGGAAGA TATTCAAACT 
TCAACACCAA CAGAAAATAA AATCAGCGAT ATCTCTCTAA AAGTTAAAGA GGTCATAGTT 
GTAAAAAAAC TGCTAACTCC AAATCCTGAT GCCATGATAA CAACAAATGT TTCCCTACAC 
TCATCTGTAC CACTTGCAAA GCCAGCCCTC TCTGAAGAAT CTAAAGAAAA GACATTATCA 
ATACTAAAGC GCACATCTAG CCCAGACATT TCCACACCAC CTGTAAAAAA GAAGAGAGGT 
CCAAAGCCCA AACTAAGAAA CTTACCATCT CAGTCAGTCC TGTTTGAAGA GATTCTACCA 
GCACGCAAAG AGAAAAGTGT CCGAGGCCCC AGAAGAAAGC GAGGACCACC CAGGAAAGCT 
TCTCTTGTCA GTGCCTCAAC AAAAGATGCT CCTCCTAACA GCAGTGGATC TACCAACAGT 
GACATGCCTG TGGTGCCTCC TCAGTGCCCC ACCAAAACAA AAGTTCTCCC CCCACGCAAA 
GGCAGAGGAC AGAAATATGA GGCAATGGTA CAGAAGATTA CATCTCCCAG CTCAAAAAAA 
CACTTCCCAG CTACGCAACA AGACAGTAGT CTAAGTGAGG ATTTTACGTC GAAGGCTTTT 
TCTGAATTTG TCTTTAAGGA AGGTGAGGCT TTTATTCAAA TAAATAACAC AGAGACACTA 
AAGTTTACAA AGGAGGAAAT GAAAGAGCAA GAAGAAGCAA TGACAATGGA AGATGTATCA 
CAACAATGTG GCAAGCATGA GTTAAGTCAA GAGGAATCAG CAAAGGATAT AGTGAGACCA 
TTAGCGGAAG AAGATGTTAC AAATAAGAAT TATGGAAGAC AAGAAAAAGG TAAAGTAGAA 
ACCAAGATAG AGGTTGCTGA CAATAAGGCT TCGTCTTCAA CAGAGACATC ATTAGAAGTT 
GGAACTCTAG ACGAGAGGAC ACAACAGACT TCCAAGAGTG ATGCCACAAA GTCTGTCCGA 
ACGAAGAGAA AGAGATGGGC CATGGTGGAA AGTACAGATG CATCAGTGGT AGCTTTGGAA 
GCAGACAGCC TCATTATTAC AACACCAAGA CTTGCTAAAC AGAGAGCTAT TAAGAACAAC 
CATGAAATGC ACCTTAAACA GAGGAGGAAG AAGAGAAAGG GCAAAGCCAA TTTAGAGGAA 
ACTGAGACAC TAGATGAGCT CAACACAACA GAAACGCCAG AACCTGTGGA AAGAATAGAG 
GAAAAAGTCA CCACCACAAA GTCCACAGAA CCTCTACCAA CTAACGGGGA TAAATTCACA 
GAAACCCCCC AAGCCATCTC TGTAGAACTT ATTCAAAAAC CTCGAAGAGG CCGAAAACCA 
TCCGCAAACC CAACTAAACA AAGAAGAAGC CAAGCCTCAA TGGAGCATGC TCCTGGGAAG 
CCAGTGAAGA TCCACAAAAA ACCTGGGCCA AAACCTGGAA TGAAAGATGC CATTGAGGTA 
ATTGAGGCAG TTGTAAGGGC AGCCGGATGT GAGCAGGACC AAAAAGAAGA AAGAGAAAAA 
AGAGAACGAG AGAATATAGA AAGTGAAGAG ACTAGTATTG TGGGTCCTGT GGTGACCGTT 
ACAGAAAAGC AGATGGAGTT CATTTCTGTG AAAAGAATCA GAAGGAGACC AATCAACAGG 
AATTCCAAAC TATCTTTTTG TCCTTATGTA CGTATCAATA ACTCTAGAGA CTTTTCTTCT 
TGGTGTGCCA TAGTCAACAA GCCTGAAGAC GCTGTTGTAT TTCAGAGACG GAGAAAAAAG 
GGCATACTTA GAATGAGGAA TCCTTTCACA GTTGCTAAAG TAGTGCCACA TACTGCTGCC 
ATGCAACTAG GACCATTGGT AAACACAAAT CTATCTGAAA TGCGTCTTAT GTGTTGCTTG 
TGTGGGAAAC CACCAAACTA CAAAGACCTG GGTGATTTGT GTGGACCATA CTACACAGAA 
GATAGTATTC CACGGAAAAT GTTGACTATC ACACATACGC AACTCTTCAG GGAAGATGAC 
AGCAAGACCC ATGAGAATGA CAGTAACAGC AGTGAAGAAC CAGGGACGTC CTCAAAGACT 
GAAGATAGAG TGAACTCAGA AAAGGAGGGA AATACAGAAG CTACCACTGA AGAGGCTGGA 
AACAGCAGGC ACCATCACCG GCACTACAGA CGGCTACTGA GACCAGAAAG ACCGTGTCAG 
GAAGGTGGTC CGAGAAGAGT TACCCTCAGA CAGAGATTCA GGAGAATGAA GCAGCTCCAG 
AATCTCTGCA CGGCCACTTC AAAAGACCAA GAGGGCAATG AAAGCATGTT CCAAAAGCTA 
CAAATGGAGG CAGTGGCCAA GGAGCACTGG GCTCATGAAA ACTGTACCAT CTGGACCAAG 
GGGATAATTA TGGTAGCTGG AAGGCTGTAT GGACTGAAGG AAGCTGCCAA CAAGTCAGTT 
CAAACGAGCT GTCACAAGTG CCAGATTTCG GGAGCGTCTC TCAGCTGCTG TTGGAGAGGC 
TGCTCTTATA AGTACCACTA CATCTGCGCC AAAGAGATAG GCTGCACATT TCATGAGGAT 
GATTTTTCCA TCAAATGCCC AAAACATGAG GTAAGACAAG AAAACTGTTA CTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RAI1 ( XM_014472413.2 ) cDNA ORF clone, Xiphophorus maculatus(Southern platyfish) -> XP_014327899.2 Xiphophorus maculatus retinoic acid induced 1 (rai1), transcript variant X2, mRNA.

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CloneID	OXi26247
Clone ID Related Accession (Same CDS sequence)	XM_014472413.2
Accession Version	XM_014472413.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6579bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1513785600000
Organism	Xiphophorus maculatus(southern platyfish)
Product	retinoic acid-induced protein 1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_036458.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Dec 22, 2017 this sequence version replaced XM_014472413.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xiphophorus maculatus Annotation Release 102 Annotation Version :: 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAGTCCT TTCGTGAGCG CAACAGTGGT TACCACAGCA ACCAACCCTG CTATCAGCAG 
GAGCCCCATG AATTATCTCG CCTGGAGACC TACCGGCAAC ACCCACATCA TCCCCATCCG 
CAGCATCCAC ACCCAGGCCC TGGTCCACAT CCAGGCCCTG GCCCTGGGCA CAACAGGTCA 
GGCTATGAGG CTATGTCTAA TGCAGCAAAC GTGCCCACAG GTGGAGCAGC TGGAATTGGA 
GGACCCAAGG ACTGTTACAG CCAACAAAAC TATTCTGGGT ACCCAGGAAG TACTGGAGGC 
AGTGGAAATG GAAATGGTAG CTCATCAGCC TCACAGGCAA AAAAATCCTT TAGAGGAAGC 
AAAGTTCCCC CACCAAACCC CAGTCAACAC CTGCAGGGCC CTGGAGGCTA TAGTAATCAC 
ATGGGCCCAG GAAATTATTC TGCCCAGTAC ATGAGCGAGG GCCATCTCCA GCAGAAATGG 
GAGGACCCTG CACAGTTAGC ACAGTATGAA CAGGAGATGG TGGGGCGACT GGAGGCTGGT 
GCTTCCCCTG CACCTGGATC CTCCCAGTAC ATAGACTCAA ACATGTTGGG GCACTCCCAA 
ACCCCATGCC ACCAGCCTTC TACCCCAAGT TATACAAGTC CACATCACCA GTCTCACCCT 
CCTAATCCTG CTCCCTCACC TCTCATGTAT CCCCAAAGTC ACCTACACTA TCCTCAACCC 
TCCCCCTCTC CATCCCCATA CATGGAAAAA TGCAGTCCCA TGCCCCATTG TTATAAAGGC 
TACAACATAC CTCCAAATTC TCAGTATAGC AGACAGCTAA GTAGTCATAG CAGTTTGAAG 
CAGGGGACTT ACAGGTCAAC CCAGAACAGC TATGGATACC AGCAGTCAAC ACCCAGAGGT 
TATGAGCAGC AGACATCTTT ACAGGCAATG ACCAACCCCC AAGAACCCCA CCCTAAATAC 
CAACACTATA GCCAACCCCA ACAAAATTAC TGTCTGTCAG AGCTCCCTGT CAGATCTCCA 
GAACAGTATT ATCAGACTTG CAGCCCCTCC TCAAGTCATT CCCCTGCTCG CTCTGTCGGA 
CGCTCACCCT CATACAGTTC CACCCCTTCA CCACTGATGA CTAATCCAGA ATCATTCCAG 
TATGGCCAAC CACCAATGAC ACCTGGGGCA GCCTCGTCGT CGTCATCCTC TTCAGCTGGT 
ATGCAAGATC AAGCCAGCAC AAACACCATG CTAATGCCCC CACGGTCCCA CCCTTCACCC 
AACATATCCC ACACAGCATC CCACAGCTAC ACTTCAACGC CACAAGCGCC CACAATGAAA 
GAGCGCTTCT CAGAGAAGCT GTTATCAAAC CCCAGCTTGT GGAGCCTAAA TGCTCTCACC 
TCTCAGGTGG AGAATATCTC CAATAACGTT CAACAGCTCC TGCTTTCAGA AGCCCTGGTT 
GCAAACAAGA AGGGCAGCAA GCGCAGCAGT GGAGGGAGCA ACTGCAGCAC TGGAAGTGGG 
GCCTCCACCA AGAAAGGTGA AGAATACAAA AGCCCTCCAT ATCCAGACAG TGGTGGCAAT 
GTGGGTGGGG CCCCCATGCA TGAACCTTAC TCTACCCCAC AGCACCAACC ATTGCCTATG 
GAACTCCATG AGGGTGGCTA CTCCAGCAGT AGTGATGAAC CATTGGACAG AGGTTTCTAT 
TACTTTGGCC AGGGTAGAAG TCCAGCCCAG GCCCACAACA ACACACAACT CAGCCTGGAC 
ACAGCCTCCT CTTGCTCCAT TGCCTCTCCA GATGATATGT CTACTAGGTC TGGGGACTCG 
GGGTTGCACA ACCTTACCCC TGATCCTATA AGATGCACGG CAGGGCAAGG AGGAGATGGC 
ATGAATACCC CAGTGAAGAG CATTGGTGAT GAGAGATCTC CAACAAGCAT TACAATACCA 
AGTCCTATGA AACAAGAAAG TGATTCCCCA TCAGACACAC AACACATCAA TCAGCCTGTA 
AAAGAGAACT TTGAAGAGTC AGCTTGGACT GAAAAACCTG CTGGAAAAGA AGAGGAAGCA 
ATGGAAAAAA CTCTTGATTT TATAAGTAAT TCAGACTCAA GCAACCCTAC AGAGAAGCAA 
GGGAAATGGA CTGATGAGGA AAAATGCCCA AATTTCTACA GCAAAATAAA CACGAACATG 
GCAGAAAAAG GCTATTGTTA TGAAGACACA ATGTACCAAG AGGTCCAGAG CAAATATGAC 
TCAGATGCAA GAGATTCCGT TGAACAGTCC CCAGCTGATC TCTCTGATTC CAGTAACAAA 
GATCACTTTA GTCAAGAGAT GAAGTCAGGG ATATTCAAAT CTGAATCTCC AACTGCATCT 
GAGAGCTCTG TGAAAACACT GCCATTCATT TCAAGAGGAG ACCTTGAGCA GGATCCATAT 
TCCACAGAGA AAGATGACAG TTCAGCAAAT ACCTCTCCAA CCCCACAAAC TGAAGTCCTG 
GAAGCTTCTA GCTGTGACAA GAAGGAGAGC AGAACTCAGG AGAATGACAA AGACGTAGTA 
GGAGATAGGG AAGAGGATGA GATAAATGAA GATGAGAAAG AAGAAATTGA AAAAAGTTCT 
CTTTCACAGC TTTTGTCTAA AGAAGTTAGG GAGGAAGCAG AGGAGGAGGA GAAAGAAAGT 
CAGTCATTAA CTGAAGAGCA CACAGATGAC AAAGACATCC AAATGAGTCC CTCTGAAGAT 
CTCTGCAGCA GAACAGAAAT CCACAATTTA GCTCTTAAAA CTGACAATGA TTTTGCAGGG 
GGACCCGGTC ATCTTAATGC AGCAGCAGCA ACGACAGCAA CAGATGTTTC AACAAGGGAG 
TCAGCCATTG GCGACACAGC ACCTCAGCCA CAGTCCGCTA TGCCAGTCTT CTCTGCTCTC 
AATGATAAGG CAGCTCCTCC TGCTCAGGCT AGAGATCATA TTGATCACAG CGATGCTAAG 
GTTCTGGAGC CAGACTCTCC TCAGCTGCCA GGGAAGTCAA TACTTCCCTC TGCCCCTTCC 
TGGGCTGACA CTCCACCATC TCCTCAAAAA GGGGATGATG ATATAGAACC AGGCATAAGT 
TGTTCCAGTG CTGTGACACC ACTAGCTAAA CCAGAGCCTA TGGTTCCATC TGCTCAGCCA 
AGGACATATG GGCGTAAGCA TGTTAGGGGT AGAAGGAGAA TCATACATTC AGGTGTGGCA 
TTAAGGCGAC AACTGAGCTT GAAGAAGGAG GAAGAAAAGG AGGACAAAGA AACAAATTCC 
TCTCAGCAAA AATCGAGCAT GGCTCCAAGC AAGACTGTTT TATTCTCAGA TCAAACTAAC 
CTTGCTCATC AGGATTCTAT GGTGAGCCAG ACTCCTAAAA TGCTGACAGA TGGTTTTCAA 
TCCAGAATGT GCACTCGCTC TTTTAATGCA CCAGATATGC AAACAAAAGT TGAACCTAAT 
GTGAAGAGAA AACCAGGCCC AAAACCTGGA TCTAAGCCAG GGTCAAAACC CAGTGCAAAA 
CCAGGGCCTA AGCCAGGTGC TAAGCCTGGT CTAAAGTCAG GTCCCAAACC TGGACCAAAA 
CCTGGGAAAA AGACAGGACA AAAGCCTGCA TCACGTGAAT CAGAAGTGCC ACTGAAAATT 
GATACTGTTG TAAGGCAAAA ACCAGGGCCA AAACCAGGTT CAAAATCTGG ACCAAAACCA 
GCCCTAAGAC CAGGTCTAAA ACCTGGATTC AAGCCTGCTG AGGTTTTGCC TCCTACTGAG 
GCTGCTCCCA TCAAAGCTCC AGTTGGTCGC CCCAGGGGCT CAATTTCAAA ATCAAAATTG 
ATGGACCGGG AAGATCCAAG ACAACAAGTC CCAGAAATGC AAAGCAGGAC CAGGAAAGCT 
CTAAAACATG CAACACTGCA ATATAACCAA GACGGAAGAT CTCCTACAGA GGAAAAGCGA 
TCTCTCCCAG AAATAAAAGT ACAAGAAAGG GAAAACAACA ATATGGTCTT AAGATCCAGA 
AAAGTCTCAC AGGATAAGCT AACAAAAGAA AAGGAAAGGG GAGGGGAAGA TATTCAAACT 
TCAACACCAA CAGAAAATAA AATCAGCGAT ATCTCTCTAA AAGTTAAAGA GGTCATAGTT 
GTAAAAAAAC TGCTAACTCC AAATCCTGAT GCCATGATAA CAACAAATGT TTCCCTACAC 
TCATCTGTAC CACTTGCAAA GCCAGCCCTC TCTGAAGAAT CTAAAGAAAA GACATTATCA 
ATACTAAAGC GCACATCTAG CCCAGACATT TCCACACCAC CTGTAAAAAA GAAGAGAGGT 
CCAAAGCCCA AACTAAGAAA CTTACCATCT CAGTCAGTCC TGTTTGAAGA GATTCTACCA 
GCACGCAAAG AGAAAAGTGT CCGAGGCCCC AGAAGAAAGC GAGGACCACC CAGGAAAGCT 
TCTCTTGTCA GTGCCTCAAC AAAAGATGCT CCTCCTAACA GCAGTGGATC TACCAACAGT 
GACATGCCTG TGGTGCCTCC TCAGTGCCCC ACCAAAACAA AAGTTCTCCC CCCACGCAAA 
GGCAGAGGAC AGAAATATGA GGCAATGGTA CAGAAGATTA CATCTCCCAG CTCAAAAAAA 
CACTTCCCAG CTACGCAACA AGACAGTAGT CTAAGTGAGG ATTTTACGTC GAAGGCTTTT 
TCTGAATTTG TCTTTAAGGA AGGTGAGGCT TTTATTCAAA TAAATAACAC AGAGACACTA 
AAGTTTACAA AGGAGGAAAT GAAAGAGCAA GAAGAAGCAA TGACAATGGA AGATGTATCA 
CAACAATGTG GCAAGCATGA GTTAAGTCAA GAGGAATCAG CAAAGGATAT AGTGAGACCA 
TTAGCGGAAG AAGATGTTAC AAATAAGAAT TATGGAAGAC AAGAAAAAGG TAAAGTAGAA 
ACCAAGATAG AGGTTGCTGA CAATAAGGCT TCGTCTTCAA CAGAGACATC ATTAGAAGTT 
GGAACTCTAG ACGAGAGGAC ACAACAGACT TCCAAGAGTG ATGCCACAAA GTCTGTCCGA 
ACGAAGAGAA AGAGATGGGC CATGGTGGAA AGTACAGATG CATCAGTGGT AGCTTTGGAA 
GCAGACAGCC TCATTATTAC AACACCAAGA CTTGCTAAAC AGAGAGCTAT TAAGAACAAC 
CATGAAATGC ACCTTAAACA GAGGAGGAAG AAGAGAAAGG GCAAAGCCAA TTTAGAGGAA 
ACTGAGACAC TAGATGAGCT CAACACAACA GAAACGCCAG AACCTGTGGA AAGAATAGAG 
GAAAAAGTCA CCACCACAAA GTCCACAGAA CCTCTACCAA CTAACGGGGA TAAATTCACA 
GAAACCCCCC AAGCCATCTC TGTAGAACTT ATTCAAAAAC CTCGAAGAGG CCGAAAACCA 
TCCGCAAACC CAACTAAACA AAGAAGAAGC CAAGCCTCAA TGGAGCATGC TCCTGGGAAG 
CCAGTGAAGA TCCACAAAAA ACCTGGGCCA AAACCTGGAA TGAAAGATGC CATTGAGGTA 
ATTGAGGCAG TTGTAAGGGC AGCCGGATGT GAGCAGGACC AAAAAGAAGA AAGAGAAAAA 
AGAGAACGAG AGAATATAGA AAGTGAAGAG ACTAGTATTG TGGGTCCTGT GGTGACCGTT 
ACAGAAAAGC AGATGGAGTT CATTTCTGTG AAAAGAATCA GAAGGAGACC AATCAACAGG 
AATTCCAAAC TATCTTTTTG TCCTTATGTA CGTATCAATA ACTCTAGAGA CTTTTCTTCT 
TGGTGTGCCA TAGTCAACAA GCCTGAAGAC GCTGTTGTAT TTCAGAGACG GAGAAAAAAG 
GGCATACTTA GAATGAGGAA TCCTTTCACA GTTGCTAAAG TAGTGCCACA TACTGCTGCC 
ATGCAACTAG GACCATTGGT AAACACAAAT CTATCTGAAA TGCGTCTTAT GTGTTGCTTG 
TGTGGGAAAC CACCAAACTA CAAAGACCTG GGTGATTTGT GTGGACCATA CTACACAGAA 
GATAGTATTC CACGGAAAAT GTTGACTATC ACACATACGC AACTCTTCAG GGAAGATGAC 
AGCAAGACCC ATGAGAATGA CAGTAACAGC AGTGAAGAAC CAGGGACGTC CTCAAAGACT 
GAAGATAGAG TGAACTCAGA AAAGGAGGGA AATACAGAAG CTACCACTGA AGAGGCTGGA 
AACAGCAGGC ACCATCACCG GCACTACAGA CGGCTACTGA GACCAGAAAG ACCGTGTCAG 
GAAGGTGGTC CGAGAAGAGT TACCCTCAGA CAGAGATTCA GGAGAATGAA GCAGCTCCAG 
AATCTCTGCA CGGCCACTTC AAAAGACCAA GAGGGCAATG AAAGCATGTT CCAAAAGCTA 
CAAATGGAGG CAGTGGCCAA GGAGCACTGG GCTCATGAAA ACTGTACCAT CTGGACCAAG 
GGGATAATTA TGGTAGCTGG AAGGCTGTAT GGACTGAAGG AAGCTGCCAA CAAGTCAGTT 
CAAACGAGCT GTCACAAGTG CCAGATTTCG GGAGCGTCTC TCAGCTGCTG TTGGAGAGGC 
TGCTCTTATA AGTACCACTA CATCTGCGCC AAAGAGATAG GCTGCACATT TCATGAGGAT 
GATTTTTCCA TCAAATGCCC AAAACATGAG GATCTGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_014327899.2
CDS	747..7325
Translation	MQSFRERNSGYHSNQPCYQQEPHELSRLETYRQHPHHPHPQHPHPGPGPHPGPGPGHNRSGYEAMSNAANVPTGGAAGIGGPKDCYSQQNYSGYPGSTGGSGNGNGSSSASQAKKSFRGSKVPPPNPSQHLQGPGGYSNHMGPGNYSAQYMSEGHLQQKWEDPAQLAQYEQEMVGRLEAGASPAPGSSQYIDSNMLGHSQTPCHQPSTPSYTSPHHQSHPPNPAPSPLMYPQSHLHYPQPSPSPSPYMEKCSPMPHCYKGYNIPPNSQYSRQLSSHSSLKQGTYRSTQNSYGYQQSTPRGYEQQTSLQAMTNPQEPHPKYQHYSQPQQNYCLSELPVRSPEQYYQTCSPSSSHSPARSVGRSPSYSSTPSPLMTNPESFQYGQPPMTPGAASSSSSSSAGMQDQASTNTMLMPPRSHPSPNISHTASHSYTSTPQAPTMKERFSEKLLSNPSLWSLNALTSQVENISNNVQQLLLSEALVANKKGSKRSSGGSNCSTGSGASTKKGEEYKSPPYPDSGGNVGGAPMHEPYSTPQHQPLPMELHEGGYSSSSDEPLDRGFYYFGQGRSPAQAHNNTQLSLDTASSCSIASPDDMSTRSGDSGLHNLTPDPIRCTAGQGGDGMNTPVKSIGDERSPTSITIPSPMKQESDSPSDTQHINQPVKENFEESAWTEKPAGKEEEAMEKTLDFISNSDSSNPTEKQGKWTDEEKCPNFYSKINTNMAEKGYCYEDTMYQEVQSKYDSDARDSVEQSPADLSDSSNKDHFSQEMKSGIFKSESPTASESSVKTLPFISRGDLEQDPYSTEKDDSSANTSPTPQTEVLEASSCDKKESRTQENDKDVVGDREEDEINEDEKEEIEKSSLSQLLSKEVREEAEEEEKESQSLTEEHTDDKDIQMSPSEDLCSRTEIHNLALKTDNDFAGGPGHLNAAAATTATDVSTRESAIGDTAPQPQSAMPVFSALNDKAAPPAQARDHIDHSDAKVLEPDSPQLPGKSILPSAPSWADTPPSPQKGDDDIEPGISCSSAVTPLAKPEPMVPSAQPRTYGRKHVRGRRRIIHSGVALRRQLSLKKEEEKEDKETNSSQQKSSMAPSKTVLFSDQTNLAHQDSMVSQTPKMLTDGFQSRMCTRSFNAPDMQTKVEPNVKRKPGPKPGSKPGSKPSAKPGPKPGAKPGLKSGPKPGPKPGKKTGQKPASRESEVPLKIDTVVRQKPGPKPGSKSGPKPALRPGLKPGFKPAEVLPPTEAAPIKAPVGRPRGSISKSKLMDREDPRQQVPEMQSRTRKALKHATLQYNQDGRSPTEEKRSLPEIKVQERENNNMVLRSRKVSQDKLTKEKERGGEDIQTSTPTENKISDISLKVKEVIVVKKLLTPNPDAMITTNVSLHSSVPLAKPALSEESKEKTLSILKRTSSPDISTPPVKKKRGPKPKLRNLPSQSVLFEEILPARKEKSVRGPRRKRGPPRKASLVSASTKDAPPNSSGSTNSDMPVVPPQCPTKTKVLPPRKGRGQKYEAMVQKITSPSSKKHFPATQQDSSLSEDFTSKAFSEFVFKEGEAFIQINNTETLKFTKEEMKEQEEAMTMEDVSQQCGKHELSQEESAKDIVRPLAEEDVTNKNYGRQEKGKVETKIEVADNKASSSTETSLEVGTLDERTQQTSKSDATKSVRTKRKRWAMVESTDASVVALEADSLIITTPRLAKQRAIKNNHEMHLKQRRKKRKGKANLEETETLDELNTTETPEPVERIEEKVTTTKSTEPLPTNGDKFTETPQAISVELIQKPRRGRKPSANPTKQRRSQASMEHAPGKPVKIHKKPGPKPGMKDAIEVIEAVVRAAGCEQDQKEEREKRERENIESEETSIVGPVVTVTEKQMEFISVKRIRRRPINRNSKLSFCPYVRINNSRDFSSWCAIVNKPEDAVVFQRRRKKGILRMRNPFTVAKVVPHTAAMQLGPLVNTNLSEMRLMCCLCGKPPNYKDLGDLCGPYYTEDSIPRKMLTITHTQLFREDDSKTHENDSNSSEEPGTSSKTEDRVNSEKEGNTEATTEEAGNSRHHHRHYRRLLRPERPCQEGGPRRVTLRQRFRRMKQLQNLCTATSKDQEGNESMFQKLQMEAVAKEHWAHENCTIWTKGIIMVAGRLYGLKEAANKSVQTSCHKCQISGASLSCCWRGCSYKYHYICAKEIGCTFHEDDFSIKCPKHEDL

Target ORF information:

RefSeq Version	XM_014472413.2
Organism	Xiphophorus maculatus(southern platyfish)
Definition	Xiphophorus maculatus retinoic acid induced 1 (rai1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014472413.2

ORF Insert Sequence:

ATGCAGTCCT TTCGTGAGCG CAACAGTGGT TACCACAGCA ACCAACCCTG CTATCAGCAG 
GAGCCCCATG AATTATCTCG CCTGGAGACC TACCGGCAAC ACCCACATCA TCCCCATCCG 
CAGCATCCAC ACCCAGGCCC TGGTCCACAT CCAGGCCCTG GCCCTGGGCA CAACAGGTCA 
GGCTATGAGG CTATGTCTAA TGCAGCAAAC GTGCCCACAG GTGGAGCAGC TGGAATTGGA 
GGACCCAAGG ACTGTTACAG CCAACAAAAC TATTCTGGGT ACCCAGGAAG TACTGGAGGC 
AGTGGAAATG GAAATGGTAG CTCATCAGCC TCACAGGCAA AAAAATCCTT TAGAGGAAGC 
AAAGTTCCCC CACCAAACCC CAGTCAACAC CTGCAGGGCC CTGGAGGCTA TAGTAATCAC 
ATGGGCCCAG GAAATTATTC TGCCCAGTAC ATGAGCGAGG GCCATCTCCA GCAGAAATGG 
GAGGACCCTG CACAGTTAGC ACAGTATGAA CAGGAGATGG TGGGGCGACT GGAGGCTGGT 
GCTTCCCCTG CACCTGGATC CTCCCAGTAC ATAGACTCAA ACATGTTGGG GCACTCCCAA 
ACCCCATGCC ACCAGCCTTC TACCCCAAGT TATACAAGTC CACATCACCA GTCTCACCCT 
CCTAATCCTG CTCCCTCACC TCTCATGTAT CCCCAAAGTC ACCTACACTA TCCTCAACCC 
TCCCCCTCTC CATCCCCATA CATGGAAAAA TGCAGTCCCA TGCCCCATTG TTATAAAGGC 
TACAACATAC CTCCAAATTC TCAGTATAGC AGACAGCTAA GTAGTCATAG CAGTTTGAAG 
CAGGGGACTT ACAGGTCAAC CCAGAACAGC TATGGATACC AGCAGTCAAC ACCCAGAGGT 
TATGAGCAGC AGACATCTTT ACAGGCAATG ACCAACCCCC AAGAACCCCA CCCTAAATAC 
CAACACTATA GCCAACCCCA ACAAAATTAC TGTCTGTCAG AGCTCCCTGT CAGATCTCCA 
GAACAGTATT ATCAGACTTG CAGCCCCTCC TCAAGTCATT CCCCTGCTCG CTCTGTCGGA 
CGCTCACCCT CATACAGTTC CACCCCTTCA CCACTGATGA CTAATCCAGA ATCATTCCAG 
TATGGCCAAC CACCAATGAC ACCTGGGGCA GCCTCGTCGT CGTCATCCTC TTCAGCTGGT 
ATGCAAGATC AAGCCAGCAC AAACACCATG CTAATGCCCC CACGGTCCCA CCCTTCACCC 
AACATATCCC ACACAGCATC CCACAGCTAC ACTTCAACGC CACAAGCGCC CACAATGAAA 
GAGCGCTTCT CAGAGAAGCT GTTATCAAAC CCCAGCTTGT GGAGCCTAAA TGCTCTCACC 
TCTCAGGTGG AGAATATCTC CAATAACGTT CAACAGCTCC TGCTTTCAGA AGCCCTGGTT 
GCAAACAAGA AGGGCAGCAA GCGCAGCAGT GGAGGGAGCA ACTGCAGCAC TGGAAGTGGG 
GCCTCCACCA AGAAAGGTGA AGAATACAAA AGCCCTCCAT ATCCAGACAG TGGTGGCAAT 
GTGGGTGGGG CCCCCATGCA TGAACCTTAC TCTACCCCAC AGCACCAACC ATTGCCTATG 
GAACTCCATG AGGGTGGCTA CTCCAGCAGT AGTGATGAAC CATTGGACAG AGGTTTCTAT 
TACTTTGGCC AGGGTAGAAG TCCAGCCCAG GCCCACAACA ACACACAACT CAGCCTGGAC 
ACAGCCTCCT CTTGCTCCAT TGCCTCTCCA GATGATATGT CTACTAGGTC TGGGGACTCG 
GGGTTGCACA ACCTTACCCC TGATCCTATA AGATGCACGG CAGGGCAAGG AGGAGATGGC 
ATGAATACCC CAGTGAAGAG CATTGGTGAT GAGAGATCTC CAACAAGCAT TACAATACCA 
AGTCCTATGA AACAAGAAAG TGATTCCCCA TCAGACACAC AACACATCAA TCAGCCTGTA 
AAAGAGAACT TTGAAGAGTC AGCTTGGACT GAAAAACCTG CTGGAAAAGA AGAGGAAGCA 
ATGGAAAAAA CTCTTGATTT TATAAGTAAT TCAGACTCAA GCAACCCTAC AGAGAAGCAA 
GGGAAATGGA CTGATGAGGA AAAATGCCCA AATTTCTACA GCAAAATAAA CACGAACATG 
GCAGAAAAAG GCTATTGTTA TGAAGACACA ATGTACCAAG AGGTCCAGAG CAAATATGAC 
TCAGATGCAA GAGATTCCGT TGAACAGTCC CCAGCTGATC TCTCTGATTC CAGTAACAAA 
GATCACTTTA GTCAAGAGAT GAAGTCAGGG ATATTCAAAT CTGAATCTCC AACTGCATCT 
GAGAGCTCTG TGAAAACACT GCCATTCATT TCAAGAGGAG ACCTTGAGCA GGATCCATAT 
TCCACAGAGA AAGATGACAG TTCAGCAAAT ACCTCTCCAA CCCCACAAAC TGAAGTCCTG 
GAAGCTTCTA GCTGTGACAA GAAGGAGAGC AGAACTCAGG AGAATGACAA AGACGTAGTA 
GGAGATAGGG AAGAGGATGA GATAAATGAA GATGAGAAAG AAGAAATTGA AAAAAGTTCT 
CTTTCACAGC TTTTGTCTAA AGAAGTTAGG GAGGAAGCAG AGGAGGAGGA GAAAGAAAGT 
CAGTCATTAA CTGAAGAGCA CACAGATGAC AAAGACATCC AAATGAGTCC CTCTGAAGAT 
CTCTGCAGCA GAACAGAAAT CCACAATTTA GCTCTTAAAA CTGACAATGA TTTTGCAGGG 
GGACCCGGTC ATCTTAATGC AGCAGCAGCA ACGACAGCAA CAGATGTTTC AACAAGGGAG 
TCAGCCATTG GCGACACAGC ACCTCAGCCA CAGTCCGCTA TGCCAGTCTT CTCTGCTCTC 
AATGATAAGG CAGCTCCTCC TGCTCAGGCT AGAGATCATA TTGATCACAG CGATGCTAAG 
GTTCTGGAGC CAGACTCTCC TCAGCTGCCA GGGAAGTCAA TACTTCCCTC TGCCCCTTCC 
TGGGCTGACA CTCCACCATC TCCTCAAAAA GGGGATGATG ATATAGAACC AGGCATAAGT 
TGTTCCAGTG CTGTGACACC ACTAGCTAAA CCAGAGCCTA TGGTTCCATC TGCTCAGCCA 
AGGACATATG GGCGTAAGCA TGTTAGGGGT AGAAGGAGAA TCATACATTC AGGTGTGGCA 
TTAAGGCGAC AACTGAGCTT GAAGAAGGAG GAAGAAAAGG AGGACAAAGA AACAAATTCC 
TCTCAGCAAA AATCGAGCAT GGCTCCAAGC AAGACTGTTT TATTCTCAGA TCAAACTAAC 
CTTGCTCATC AGGATTCTAT GGTGAGCCAG ACTCCTAAAA TGCTGACAGA TGGTTTTCAA 
TCCAGAATGT GCACTCGCTC TTTTAATGCA CCAGATATGC AAACAAAAGT TGAACCTAAT 
GTGAAGAGAA AACCAGGCCC AAAACCTGGA TCTAAGCCAG GGTCAAAACC CAGTGCAAAA 
CCAGGGCCTA AGCCAGGTGC TAAGCCTGGT CTAAAGTCAG GTCCCAAACC TGGACCAAAA 
CCTGGGAAAA AGACAGGACA AAAGCCTGCA TCACGTGAAT CAGAAGTGCC ACTGAAAATT 
GATACTGTTG TAAGGCAAAA ACCAGGGCCA AAACCAGGTT CAAAATCTGG ACCAAAACCA 
GCCCTAAGAC CAGGTCTAAA ACCTGGATTC AAGCCTGCTG AGGTTTTGCC TCCTACTGAG 
GCTGCTCCCA TCAAAGCTCC AGTTGGTCGC CCCAGGGGCT CAATTTCAAA ATCAAAATTG 
ATGGACCGGG AAGATCCAAG ACAACAAGTC CCAGAAATGC AAAGCAGGAC CAGGAAAGCT 
CTAAAACATG CAACACTGCA ATATAACCAA GACGGAAGAT CTCCTACAGA GGAAAAGCGA 
TCTCTCCCAG AAATAAAAGT ACAAGAAAGG GAAAACAACA ATATGGTCTT AAGATCCAGA 
AAAGTCTCAC AGGATAAGCT AACAAAAGAA AAGGAAAGGG GAGGGGAAGA TATTCAAACT 
TCAACACCAA CAGAAAATAA AATCAGCGAT ATCTCTCTAA AAGTTAAAGA GGTCATAGTT 
GTAAAAAAAC TGCTAACTCC AAATCCTGAT GCCATGATAA CAACAAATGT TTCCCTACAC 
TCATCTGTAC CACTTGCAAA GCCAGCCCTC TCTGAAGAAT CTAAAGAAAA GACATTATCA 
ATACTAAAGC GCACATCTAG CCCAGACATT TCCACACCAC CTGTAAAAAA GAAGAGAGGT 
CCAAAGCCCA AACTAAGAAA CTTACCATCT CAGTCAGTCC TGTTTGAAGA GATTCTACCA 
GCACGCAAAG AGAAAAGTGT CCGAGGCCCC AGAAGAAAGC GAGGACCACC CAGGAAAGCT 
TCTCTTGTCA GTGCCTCAAC AAAAGATGCT CCTCCTAACA GCAGTGGATC TACCAACAGT 
GACATGCCTG TGGTGCCTCC TCAGTGCCCC ACCAAAACAA AAGTTCTCCC CCCACGCAAA 
GGCAGAGGAC AGAAATATGA GGCAATGGTA CAGAAGATTA CATCTCCCAG CTCAAAAAAA 
CACTTCCCAG CTACGCAACA AGACAGTAGT CTAAGTGAGG ATTTTACGTC GAAGGCTTTT 
TCTGAATTTG TCTTTAAGGA AGGTGAGGCT TTTATTCAAA TAAATAACAC AGAGACACTA 
AAGTTTACAA AGGAGGAAAT GAAAGAGCAA GAAGAAGCAA TGACAATGGA AGATGTATCA 
CAACAATGTG GCAAGCATGA GTTAAGTCAA GAGGAATCAG CAAAGGATAT AGTGAGACCA 
TTAGCGGAAG AAGATGTTAC AAATAAGAAT TATGGAAGAC AAGAAAAAGG TAAAGTAGAA 
ACCAAGATAG AGGTTGCTGA CAATAAGGCT TCGTCTTCAA CAGAGACATC ATTAGAAGTT 
GGAACTCTAG ACGAGAGGAC ACAACAGACT TCCAAGAGTG ATGCCACAAA GTCTGTCCGA 
ACGAAGAGAA AGAGATGGGC CATGGTGGAA AGTACAGATG CATCAGTGGT AGCTTTGGAA 
GCAGACAGCC TCATTATTAC AACACCAAGA CTTGCTAAAC AGAGAGCTAT TAAGAACAAC 
CATGAAATGC ACCTTAAACA GAGGAGGAAG AAGAGAAAGG GCAAAGCCAA TTTAGAGGAA 
ACTGAGACAC TAGATGAGCT CAACACAACA GAAACGCCAG AACCTGTGGA AAGAATAGAG 
GAAAAAGTCA CCACCACAAA GTCCACAGAA CCTCTACCAA CTAACGGGGA TAAATTCACA 
GAAACCCCCC AAGCCATCTC TGTAGAACTT ATTCAAAAAC CTCGAAGAGG CCGAAAACCA 
TCCGCAAACC CAACTAAACA AAGAAGAAGC CAAGCCTCAA TGGAGCATGC TCCTGGGAAG 
CCAGTGAAGA TCCACAAAAA ACCTGGGCCA AAACCTGGAA TGAAAGATGC CATTGAGGTA 
ATTGAGGCAG TTGTAAGGGC AGCCGGATGT GAGCAGGACC AAAAAGAAGA AAGAGAAAAA 
AGAGAACGAG AGAATATAGA AAGTGAAGAG ACTAGTATTG TGGGTCCTGT GGTGACCGTT 
ACAGAAAAGC AGATGGAGTT CATTTCTGTG AAAAGAATCA GAAGGAGACC AATCAACAGG 
AATTCCAAAC TATCTTTTTG TCCTTATGTA CGTATCAATA ACTCTAGAGA CTTTTCTTCT 
TGGTGTGCCA TAGTCAACAA GCCTGAAGAC GCTGTTGTAT TTCAGAGACG GAGAAAAAAG 
GGCATACTTA GAATGAGGAA TCCTTTCACA GTTGCTAAAG TAGTGCCACA TACTGCTGCC 
ATGCAACTAG GACCATTGGT AAACACAAAT CTATCTGAAA TGCGTCTTAT GTGTTGCTTG 
TGTGGGAAAC CACCAAACTA CAAAGACCTG GGTGATTTGT GTGGACCATA CTACACAGAA 
GATAGTATTC CACGGAAAAT GTTGACTATC ACACATACGC AACTCTTCAG GGAAGATGAC 
AGCAAGACCC ATGAGAATGA CAGTAACAGC AGTGAAGAAC CAGGGACGTC CTCAAAGACT 
GAAGATAGAG TGAACTCAGA AAAGGAGGGA AATACAGAAG CTACCACTGA AGAGGCTGGA 
AACAGCAGGC ACCATCACCG GCACTACAGA CGGCTACTGA GACCAGAAAG ACCGTGTCAG 
GAAGGTGGTC CGAGAAGAGT TACCCTCAGA CAGAGATTCA GGAGAATGAA GCAGCTCCAG 
AATCTCTGCA CGGCCACTTC AAAAGACCAA GAGGGCAATG AAAGCATGTT CCAAAAGCTA 
CAAATGGAGG CAGTGGCCAA GGAGCACTGG GCTCATGAAA ACTGTACCAT CTGGACCAAG 
GGGATAATTA TGGTAGCTGG AAGGCTGTAT GGACTGAAGG AAGCTGCCAA CAAGTCAGTT 
CAAACGAGCT GTCACAAGTG CCAGATTTCG GGAGCGTCTC TCAGCTGCTG TTGGAGAGGC 
TGCTCTTATA AGTACCACTA CATCTGCGCC AAAGAGATAG GCTGCACATT TCATGAGGAT 
GATTTTTCCA TCAAATGCCC AAAACATGAG GATCTGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

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RAI1 cDNA ORF clone, Xiphophorus maculatus(Southern platyfish)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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