oplah cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

Gene
Clones
gRNAs

Summary

Gene Symbol	oplah
Entrez Gene ID	780345
Full Name	5-oxoprolinase, ATP-hydrolysing
General protein information	Preferred Names 5-oxoprolinase, ATP-hydrolysing Names 5-oxoprolinase
Gene Type	protein-coding
Organism	Xenopus tropicalis(tropical clawed frog)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001079415.1	NP_001072883.1	5-oxoprolinase
XM_012953922.3	XP_012809376.1	5-oxoprolinase isoform X1
XM_012953923.1	XP_012809377.1	5-oxoprolinase isoform X2
XM_012953922.2	XP_012809376.1	5-oxoprolinase isoform X1

Pathways from BioSystems

Homologies

Xenopus tropicalis (tropical clawed frog)	oplah	NP_001072883.1
Eremothecium gossypii	AGOS_ABR114C	NP_983061.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP001606	XP_321495.3
Magnaporthe oryzae (rice blast fungus)	MGG_00771	XP_003718224.1
Mus musculus (house mouse)	Oplah	NP_694762.1
Danio rerio (zebrafish)	si:dkey-9l5.2	NP_001186988.1
Kluyveromyces lactis	KLLA0C18975g	XP_453046.1
Caenorhabditis elegans (roundworm)	Y38F2AR.12	NP_001076725.1
Saccharomyces cerevisiae (baker's yeast)	OXP1	NP_012707.1
Arabidopsis thaliana (thale cress)	OXP1	NP_198599.1
Rattus norvegicus (Norway rat)	Oplah	NP_446356.1
Drosophila melanogaster (fruit fly)	CG4752	NP_611675.2
Pan troglodytes (chimpanzee)	OPLAH	XP_003951428.1
Schizosaccharomyces pombe (fission yeast)	SPAC11D3.15	NP_592810.1
Neurospora crassa	NCU04569	XP_958668.2
Homo sapiens (human)	OPLAH	NP_060040.1
Canis lupus familiaris (dog)	OPLAH	XP_005628367.1
Bos taurus (cattle)	OPLAH	NP_001001173.1

Gene Ontology
Bibliography

Related articles in PubMed

Genetic and genomic tools for Xenopus research: The NIH Xenopus initiative.
Klein SL, Strausberg RL, Wagner L, Pontius J, Clifton SW, Richardson P
Developmental dynamics : an official publication of the American Association of Anatomists225(4)384-91(2002 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following oplah gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the oplah cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OXa20514	NM_001079415.1 Latest version!	Xenopus tropicalis 5-oxoprolinase, ATP-hydrolysing (oplah), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OXa29703	XM_012953922.3 Latest version!	Xenopus tropicalis 5-oxoprolinase, ATP-hydrolysing (oplah), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OXa29704	XM_012953923.1 Latest version!	Xenopus tropicalis 5-oxoprolinase, ATP-hydrolysing (oplah), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
View Old Accession Versions >>
OXa29703	XM_012953922.2	Xenopus tropicalis 5-oxoprolinase (ATP-hydrolysing) (oplah), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
Hide Old Accession Versions >>

ORF Online Only Promotion

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***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

oplah ( NM_001079415.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> NP_001072883.1 Xenopus tropicalis 5-oxoprolinase, ATP-hydrolysing (oplah), mRNA.

Price & Availability↑

CloneID	OXa20514
Clone ID Related Accession (Same CDS sequence)	NM_001079415.1
Accession Version	NM_001079415.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3876bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1576944000000
Organism	Xenopus tropicalis(tropical clawed frog)
Product	5-oxoprolinase
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC125692.1. ##Evidence-Data-START## Transcript exon combination :: BC125692.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMD00028290, SAMD00028291 [ECO:0000350] ##Evidence-Data-END##

ATGGCGGATA AGTTCCAGTT CGCTATAGAC CGGGGGGGCA CATTCACGGA TGTGTTTGCG 
AGATGCCCCG GGGGGAAAGT TCGTGTTATG AAACTGCTGT CCGAGGACCC AGCGAATTAC 
CAGGATGCCC CCACAGAGGG GATCAGAAGG ATACTGGAGG AGGAGACCGG ATGTTCATTC 
CCACGGGACC AACCACTGGA CACAGGGAGG ATCGAGTGGA TCCGTATGGG CACCACTGTG 
GCCACCAATG CCCTGCTGGA AAGGAAGGGG GAGAGAATTG CTCTGCTCAT CACCAAGGGC 
TTCCGGCACC TTCTGCACAT TGGGAACCAA GCCCGGCCCA AGATCTTTGA CTTGGAGATC 
CAGATGCCCG AGGTGCTGTA CGAGGAAGTG ATAGAGGTGG AGGAGAGGGT GGTACTGACC 
CAGGGAGGCT GCTGTCTCCC AAAGGGCGAA CCCACGGCGC CCCCTGTAGG TTCTACAGGA 
GAGTGTCTGG AGGTGTGGGA GCCGCTCAAT CTGAACCGCC TGGAGATGGA TCTTCGGAAT 
GTACTGGCCC GTGGGATCAG GAGCCTGGCT GTGGTCCTTA TGCATTCTTA CACGTGGTCT 
TCCCATGAGC ACCAAATAGG GGAACTGGCA CGCTCCTTGG GGTTCACCCA TGTGTCTCTC 
TCGTCAGAGG TGATGCCAAT GATCCGCATT GTGCCCCGGG GTTACACGGC CTGCGCTGAT 
GCCTATCTCA CCCCCTGCAT AAAGAGGTAT CTCCACGGCT TCTGCCGGGG CTTCAAGAAC 
CAACTGAAGG ACTTGCAGGT CTTATTCATG CGCTCTGATG GAGGCCTGAC CCCTATGCAG 
AACTTTAGTG GCTCCCGTGC CATTCTCTCG GGCCCCGCGG GAGGGGTTGT GGGTTATGCC 
ATCACCACAT ATCACCGCGA CCACCCGCAG CCGGTCATTG GCTTTGATAT GGGAGGGACC 
TCCACAGACG TGAGCAGATA CGCCGGGGAG TATGAACATG TATTCGAAGC CACGACGGCG 
GGAATCAGCA TCCAGGCCCC ACAGCTCGAT GTCAATACAG TGGCAGCCGG AGGGGGCTCT 
ATGCTCTTCT TCAGATCAGG TCTGTTTGTT GTGGGACCAG AGTCTGCAGG AGCCCACCCC 
GGCCCAGCCT GCTACAGGAA AGGAGGGCCA CTTACTGTGA CTGATGCCAA CCTCTGCCTG 
GGCCGCCTCC TCCCTGACTA CTTTCCCCGG ATCTTTGGCC CGTCGGAAGA CCAGCCGCTC 
TCTCGGGAAG ACACGCTGCA CCAGTTTCAG AAGTTGGCTG CTGATATTAA CCAGTTCCAG 
GCTGGGAGAC CCGGTTCTGC TGCTTCACTG AGTGTGGAGG AAGTTGCTAT GGGGTTCATC 
AGAGTGGCCA ATGAAGCCAT GTGCCGGCCA ATCAGATCTC TCACTCAGGC TAAGGGCCAC 
GACACTTCGC GCCACGTGCT GGCCTGCTTT GGGGGTGCCG GGGGGCAGCA TGCTTGTGCC 
ATCGCTCGCT CACTGGGCAT GAAAACTGTG TTTATCCACA AGTACGGCGG CGTCCTGTCT 
GCGTTTGGGA TGGCATTGGC CGATGTGGTG CACGAGGCGC AGGAGCCCTG CTCTATGGGG 
TACAGCCCCG AATCATTCTG CCTGCTGGAG GGACGCATCG CAGCCTTGGA GAGGCGCTGC 
ATAGAGGCCC TGCAGGAACA AGGCTTCCAC AGGTCTCAGA TTGACACGGA GCCATTCCTG 
CACCTGCGCT ATGAGAGAAC CGACTGCGCC CTCATGTGCT CCAACCGGGG GTACCCAGCC 
GCCCAGGGCT CCTGCCTCGT GGGCAACTTC AAACTGGCCT TCACTGACAG GTACCAGAAG 
GAGTTCGGAT TCACCATCCC CTCGCGCGCC ATTGTGGTGG ATGACATCCG AGTGCGAGGG 
ACGGGCAGCA CCGGAATCCG CTGCGAGACT GCCGTTCAGC CGAGTGGGCA GCCTGCCCGA 
GTGGAGGCAG TCGCCAGATG TTACTTTGAG GACGGACACC ATGACACAAA CGTGTACCTC 
CTGCAGGACC TCGCCCCTGA CCATTCCGTT ACGGGACCCG CCATTATCAT CGACAAGAAC 
AGTACCATCC TGGTGGAACC CGACTGCACG GCCTCTATAA CTGAATATGG AGACGTCCGG 
ATCTCCGTTG GGAGCGGAAG GCAGAGGGGG GTCGGCACGG AGTTGGACAC AATACAGCTG 
TCCATCTTCT CCCATCGGTT CATGAGCATT GCAGAGCAGA TGGGCCGTAT CCTGCAGAGA 
ACCGCCATTT CCACGAACAT CAAAGAGCGA CTAGACTTCT CCTGTGCTCT CTTTGGCCCT 
GATGGGGGCT TAGTGTCTAA CGCTCCCCAT ATACCTGTCC ATCTGGGTGC CATGCAGGAG 
ACAGTCCAGT TCCAGATCAG GAACCTGAAG GATGATCTGA AGGAAGGGGA TGTGCTACTG 
AGTAATCACC CCTGTGCCGG GGGCAGCCAC CTCCCAGACC TTACGGTTAT TACCCCTGTG 
TTCCGGGTGG AGGCCCCACG CCCAGTGTTT TTTGTGGCAT CCAGGGGCCA CCACGCCGAC 
ATTGGGGGGA TCACACCCGG CTCCATGCCA CCCCACTCCA AGACCCTGCG GGACGAGGGG 
GCAGTGTTCA CCTCATTCAA ACTGGTGCGA GGGGGGGAAT TCCAGGAGGA AGCGGTTACT 
GAGGCTCTTA TGGCTCCTGG TCTGATCCCG GGCAGCAGCG GGACGCGCAA TCTCCATGAC 
AACCTGTCCG ACCTGCGGGC GCAGGTAGCG GCCAATCAGA AAGGCATCCA GCTGTTGAAT 
GAGCTGATCG ATGTGTATGG GCTGGCCGTG GTACAGGCCT ACATGGCGCA CATTCAGGCC 
AATGCCGAGG TGGCTGTGCG GAACATGCTG AGAGACTTTG CTGAGCGCTG GGAGAGCCAC 
AAGGGGGCGC TAGAGGTGGA GTCTGTGGAT TACATGGACG ACGGTTCCCC AATTAAACTG 
AAAGTGCGGA TAAACAAGCA GGAGGGCGGC GCTCTCTTTG ATTTCACTGG ATCCGGATAT 
GAGGTGTTTG GGAACTGTAA CGCCCCGCGC GCCATTACCC TCTCTGCTCT TATTTATTGC 
CTCCGCTGCA TGGTGGGCCA AGATATCCCT CTTAATCAGG GCTGCCTGAC CCCCATCCAG 
GTTATCATTC CCAAAGGATC CATCCTGGAC CCCTCTCCTG ATGCGGCTGT GGTCGGGGGT 
AATGTGCTCA CATCGCAAAG GATCGTCGAT GTCATCTTCA AGGCCTTTGA AGTCTGTGCG 
GCATCTCAGG GCTGTATGAA CAATGTAACC TTTGGCAACA AGACTACGGG TTACTATGAG 
ACGGTGGCTG GCGGTGCAGG GGCGGGACCT CACTGGCACG GTCGGAGCGG CATCCACACT 
CACATGACCA ACACCCGCAT CACTGACCCA GAAATCTTGG AGAAACGATA CCCCGTGGTG 
CTGAAGCGCT TTGAGCTGTC CCCTGGTACC GGCGGCGCAG GGAAGTTCCG TGGCGGGGAT 
GGAGTGATCC GGGAGCTGCT GTTTAGGGAA GATATTGTTT TATCGGTGCT GACGGAGCGG 
CGAGTGTTCA GACCCTACGG GTTACAGGGG GGATGTAGTG GGGCCCCCGG GCTCAACCTC 
CTGCGCAGGA AGGATGGAAC AACCATTAAC CTCGGGGGCA AAACGTCAGT GAATGTGGAG 
CCGGGGGACA CGTTCACCCT TCACACCCCA GGAGGTGGGG GGTTTGGGGT TCAGGAGGGG 
AACCAAAGTA CCCCGGTACC CGTAAAGGTT CTCAGTCGCA GCTTTGCCGA GCGCGGGAGC 
GTGTTCGAGT ACCGCAAAGC TCAGGAAACT GTATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001072883.1
CDS	25..3900
Translation	MADKFQFAIDRGGTFTDVFARCPGGKVRVMKLLSEDPANYQDAPTEGIRRILEEETGCSFPRDQPLDTGRIEWIRMGTTVATNALLERKGERIALLITKGFRHLLHIGNQARPKIFDLEIQMPEVLYEEVIEVEERVVLTQGGCCLPKGEPTAPPVGSTGECLEVWEPLNLNRLEMDLRNVLARGIRSLAVVLMHSYTWSSHEHQIGELARSLGFTHVSLSSEVMPMIRIVPRGYTACADAYLTPCIKRYLHGFCRGFKNQLKDLQVLFMRSDGGLTPMQNFSGSRAILSGPAGGVVGYAITTYHRDHPQPVIGFDMGGTSTDVSRYAGEYEHVFEATTAGISIQAPQLDVNTVAAGGGSMLFFRSGLFVVGPESAGAHPGPACYRKGGPLTVTDANLCLGRLLPDYFPRIFGPSEDQPLSREDTLHQFQKLAADINQFQAGRPGSAASLSVEEVAMGFIRVANEAMCRPIRSLTQAKGHDTSRHVLACFGGAGGQHACAIARSLGMKTVFIHKYGGVLSAFGMALADVVHEAQEPCSMGYSPESFCLLEGRIAALERRCIEALQEQGFHRSQIDTEPFLHLRYERTDCALMCSNRGYPAAQGSCLVGNFKLAFTDRYQKEFGFTIPSRAIVVDDIRVRGTGSTGIRCETAVQPSGQPARVEAVARCYFEDGHHDTNVYLLQDLAPDHSVTGPAIIIDKNSTILVEPDCTASITEYGDVRISVGSGRQRGVGTELDTIQLSIFSHRFMSIAEQMGRILQRTAISTNIKERLDFSCALFGPDGGLVSNAPHIPVHLGAMQETVQFQIRNLKDDLKEGDVLLSNHPCAGGSHLPDLTVITPVFRVEAPRPVFFVASRGHHADIGGITPGSMPPHSKTLRDEGAVFTSFKLVRGGEFQEEAVTEALMAPGLIPGSSGTRNLHDNLSDLRAQVAANQKGIQLLNELIDVYGLAVVQAYMAHIQANAEVAVRNMLRDFAERWESHKGALEVESVDYMDDGSPIKLKVRINKQEGGALFDFTGSGYEVFGNCNAPRAITLSALIYCLRCMVGQDIPLNQGCLTPIQVIIPKGSILDPSPDAAVVGGNVLTSQRIVDVIFKAFEVCAASQGCMNNVTFGNKTTGYYETVAGGAGAGPHWHGRSGIHTHMTNTRITDPEILEKRYPVVLKRFELSPGTGGAGKFRGGDGVIRELLFREDIVLSVLTERRVFRPYGLQGGCSGAPGLNLLRRKDGTTINLGGKTSVNVEPGDTFTLHTPGGGGFGVQEGNQSTPVPVKVLSRSFAERGSVFEYRKAQETV

Target ORF information:

RefSeq Version	NM_001079415.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis 5-oxoprolinase, ATP-hydrolysing (oplah), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001079415.1

ORF Insert Sequence:

ATGGCGGATA AGTTCCAGTT CGCTATAGAC CGGGGGGGCA CATTCACGGA TGTGTTTGCG 
AGATGCCCCG GGGGGAAAGT TCGTGTTATG AAACTGCTGT CCGAGGACCC AGCGAATTAC 
CAGGATGCCC CCACAGAGGG GATCAGAAGG ATACTGGAGG AGGAGACCGG ATGTTCATTC 
CCACGGGACC AACCACTGGA CACAGGGAGG ATCGAGTGGA TCCGTATGGG CACCACTGTG 
GCCACCAATG CCCTGCTGGA AAGGAAGGGG GAGAGAATTG CTCTGCTCAT CACCAAGGGC 
TTCCGGCACC TTCTGCACAT TGGGAACCAA GCCCGGCCCA AGATCTTTGA CTTGGAGATC 
CAGATGCCCG AGGTGCTGTA CGAGGAAGTG ATAGAGGTGG AGGAGAGGGT GGTACTGACC 
CAGGGAGGCT GCTGTCTCCC AAAGGGCGAA CCCACGGCGC CCCCTGTAGG TTCTACAGGA 
GAGTGTCTGG AGGTGTGGGA GCCGCTCAAT CTGAACCGCC TGGAGATGGA TCTTCGGAAT 
GTACTGGCCC GTGGGATCAG GAGCCTGGCT GTGGTCCTTA TGCATTCTTA CACGTGGTCT 
TCCCATGAGC ACCAAATAGG GGAACTGGCA CGCTCCTTGG GGTTCACCCA TGTGTCTCTC 
TCGTCAGAGG TGATGCCAAT GATCCGCATT GTGCCCCGGG GTTACACGGC CTGCGCTGAT 
GCCTATCTCA CCCCCTGCAT AAAGAGGTAT CTCCACGGCT TCTGCCGGGG CTTCAAGAAC 
CAACTGAAGG ACTTGCAGGT CTTATTCATG CGCTCTGATG GAGGCCTGAC CCCTATGCAG 
AACTTTAGTG GCTCCCGTGC CATTCTCTCG GGCCCCGCGG GAGGGGTTGT GGGTTATGCC 
ATCACCACAT ATCACCGCGA CCACCCGCAG CCGGTCATTG GCTTTGATAT GGGAGGGACC 
TCCACAGACG TGAGCAGATA CGCCGGGGAG TATGAACATG TATTCGAAGC CACGACGGCG 
GGAATCAGCA TCCAGGCCCC ACAGCTCGAT GTCAATACAG TGGCAGCCGG AGGGGGCTCT 
ATGCTCTTCT TCAGATCAGG TCTGTTTGTT GTGGGACCAG AGTCTGCAGG AGCCCACCCC 
GGCCCAGCCT GCTACAGGAA AGGAGGGCCA CTTACTGTGA CTGATGCCAA CCTCTGCCTG 
GGCCGCCTCC TCCCTGACTA CTTTCCCCGG ATCTTTGGCC CGTCGGAAGA CCAGCCGCTC 
TCTCGGGAAG ACACGCTGCA CCAGTTTCAG AAGTTGGCTG CTGATATTAA CCAGTTCCAG 
GCTGGGAGAC CCGGTTCTGC TGCTTCACTG AGTGTGGAGG AAGTTGCTAT GGGGTTCATC 
AGAGTGGCCA ATGAAGCCAT GTGCCGGCCA ATCAGATCTC TCACTCAGGC TAAGGGCCAC 
GACACTTCGC GCCACGTGCT GGCCTGCTTT GGGGGTGCCG GGGGGCAGCA TGCTTGTGCC 
ATCGCTCGCT CACTGGGCAT GAAAACTGTG TTTATCCACA AGTACGGCGG CGTCCTGTCT 
GCGTTTGGGA TGGCATTGGC CGATGTGGTG CACGAGGCGC AGGAGCCCTG CTCTATGGGG 
TACAGCCCCG AATCATTCTG CCTGCTGGAG GGACGCATCG CAGCCTTGGA GAGGCGCTGC 
ATAGAGGCCC TGCAGGAACA AGGCTTCCAC AGGTCTCAGA TTGACACGGA GCCATTCCTG 
CACCTGCGCT ATGAGAGAAC CGACTGCGCC CTCATGTGCT CCAACCGGGG GTACCCAGCC 
GCCCAGGGCT CCTGCCTCGT GGGCAACTTC AAACTGGCCT TCACTGACAG GTACCAGAAG 
GAGTTCGGAT TCACCATCCC CTCGCGCGCC ATTGTGGTGG ATGACATCCG AGTGCGAGGG 
ACGGGCAGCA CCGGAATCCG CTGCGAGACT GCCGTTCAGC CGAGTGGGCA GCCTGCCCGA 
GTGGAGGCAG TCGCCAGATG TTACTTTGAG GACGGACACC ATGACACAAA CGTGTACCTC 
CTGCAGGACC TCGCCCCTGA CCATTCCGTT ACGGGACCCG CCATTATCAT CGACAAGAAC 
AGTACCATCC TGGTGGAACC CGACTGCACG GCCTCTATAA CTGAATATGG AGACGTCCGG 
ATCTCCGTTG GGAGCGGAAG GCAGAGGGGG GTCGGCACGG AGTTGGACAC AATACAGCTG 
TCCATCTTCT CCCATCGGTT CATGAGCATT GCAGAGCAGA TGGGCCGTAT CCTGCAGAGA 
ACCGCCATTT CCACGAACAT CAAAGAGCGA CTAGACTTCT CCTGTGCTCT CTTTGGCCCT 
GATGGGGGCT TAGTGTCTAA CGCTCCCCAT ATACCTGTCC ATCTGGGTGC CATGCAGGAG 
ACAGTCCAGT TCCAGATCAG GAACCTGAAG GATGATCTGA AGGAAGGGGA TGTGCTACTG 
AGTAATCACC CCTGTGCCGG GGGCAGCCAC CTCCCAGACC TTACGGTTAT TACCCCTGTG 
TTCCGGGTGG AGGCCCCACG CCCAGTGTTT TTTGTGGCAT CCAGGGGCCA CCACGCCGAC 
ATTGGGGGGA TCACACCCGG CTCCATGCCA CCCCACTCCA AGACCCTGCG GGACGAGGGG 
GCAGTGTTCA CCTCATTCAA ACTGGTGCGA GGGGGGGAAT TCCAGGAGGA AGCGGTTACT 
GAGGCTCTTA TGGCTCCTGG TCTGATCCCG GGCAGCAGCG GGACGCGCAA TCTCCATGAC 
AACCTGTCCG ACCTGCGGGC GCAGGTAGCG GCCAATCAGA AAGGCATCCA GCTGTTGAAT 
GAGCTGATCG ATGTGTATGG GCTGGCCGTG GTACAGGCCT ACATGGCGCA CATTCAGGCC 
AATGCCGAGG TGGCTGTGCG GAACATGCTG AGAGACTTTG CTGAGCGCTG GGAGAGCCAC 
AAGGGGGCGC TAGAGGTGGA GTCTGTGGAT TACATGGACG ACGGTTCCCC AATTAAACTG 
AAAGTGCGGA TAAACAAGCA GGAGGGCGGC GCTCTCTTTG ATTTCACTGG ATCCGGATAT 
GAGGTGTTTG GGAACTGTAA CGCCCCGCGC GCCATTACCC TCTCTGCTCT TATTTATTGC 
CTCCGCTGCA TGGTGGGCCA AGATATCCCT CTTAATCAGG GCTGCCTGAC CCCCATCCAG 
GTTATCATTC CCAAAGGATC CATCCTGGAC CCCTCTCCTG ATGCGGCTGT GGTCGGGGGT 
AATGTGCTCA CATCGCAAAG GATCGTCGAT GTCATCTTCA AGGCCTTTGA AGTCTGTGCG 
GCATCTCAGG GCTGTATGAA CAATGTAACC TTTGGCAACA AGACTACGGG TTACTATGAG 
ACGGTGGCTG GCGGTGCAGG GGCGGGACCT CACTGGCACG GTCGGAGCGG CATCCACACT 
CACATGACCA ACACCCGCAT CACTGACCCA GAAATCTTGG AGAAACGATA CCCCGTGGTG 
CTGAAGCGCT TTGAGCTGTC CCCTGGTACC GGCGGCGCAG GGAAGTTCCG TGGCGGGGAT 
GGAGTGATCC GGGAGCTGCT GTTTAGGGAA GATATTGTTT TATCGGTGCT GACGGAGCGG 
CGAGTGTTCA GACCCTACGG GTTACAGGGG GGATGTAGTG GGGCCCCCGG GCTCAACCTC 
CTGCGCAGGA AGGATGGAAC AACCATTAAC CTCGGGGGCA AAACGTCAGT GAATGTGGAG 
CCGGGGGACA CGTTCACCCT TCACACCCCA GGAGGTGGGG GGTTTGGGGT TCAGGAGGGG 
AACCAAAGTA CCCCGGTACC CGTAAAGGTT CTCAGTCGCA GCTTTGCCGA GCGCGGGAGC 
GTGTTCGAGT ACCGCAAAGC TCAGGAAACT GTATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

oplah ( XM_012953922.3 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012809376.1 Xenopus tropicalis 5-oxoprolinase, ATP-hydrolysing (oplah), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OXa29703
Clone ID Related Accession (Same CDS sequence)	XM_012953922.3 , XM_012953922.2
Accession Version	XM_012953922.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3876bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1576512000000
Organism	Xenopus tropicalis(tropical clawed frog)
Product	5-oxoprolinase isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030682.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012953922.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGATA AGTTCCAGTT CGCTATAGAC CGGGGGGGCA CATTCACGGA TGTGTTTGCG 
AGATGCCCCG GTGGGAAAGT TCGTGTTATG AAACTGCTGT CCGAGGACCC AGCGAATTAC 
CAGGATGCCC CCACAGAGGG GATCAGAAGG ATACTGGAGG AGGAGACCGG ATGTTCATTC 
CCACGGGACC AACCACTGGA CACAGGGAGG ATCGAGTGGA TCCGTATGGG CACCACTGTG 
GCCACCAATG CCCTGCTGGA AAGGAAGGGG GAGAGAATTG CTCTGCTCAT CACCAAGGGC 
TTCCGGCACC TTCTGCACAT TGGGAACCAA GCCCGGCCCA AGATCTTTGA CTTGGAGATC 
CAGATGCCCG AGGTGCTGTA CGAGGAAGTG ATAGAGGTGG AGGAGAGGGT GGTACTGACC 
CAGGGAGGCT GCTGTCTCCC AAAGGGCGAA CCCACGGCGC CCCCTGTAGG TTCTACAGGA 
GAGTGTCTGG AGGTGTGGGA GCCGCTCAAT CTGAACCGCC TGGAGATGGA TCTTCGGAAT 
GTACTGGCCC GTGGGATCAG GAGCCTGGCT GTGGTCCTTA TGCATTCTTA CACGTGGTCT 
TCCCATGAGC ACCAAATAGG GGAACTGGCA CGCTCCTTGG GGTTCACCCA TGTGTCTCTC 
TCGTCAGAGG TGATGCCAAT GATCCGCATT GTGCCCCGGG GTTACACGGC CTGCGCTGAT 
GCCTACCTCA CCCCCTGCAT AAAGAGGTAT CTCCACGGCT TCTGCCGGGG CTTCAAGAAC 
CAACTGAAGG ACTTGCAGGT CTTATTCATG CGCTCTGATG GAGGCCTGAC CCCTATGCAG 
AACTTTAGTG GCTCCCGTGC CATTCTCTCG GGCCCCGCGG GAGGGGTTGT GGGTTATGCC 
ATCACCACAT ATCACCGCGA CCACCCGCAG CCGGTCATTG GCTTTGATAT GGGAGGGACC 
TCCACAGACG TGAGCAGATA CGCCGGGGAG TATGAACATG TATTCGAAGC CACGACGGCG 
GGAATCAGCA TCCAGGCCCC ACAGCTCGAT GTCAATACAG TGGCAGCCGG AGGGGGCTCT 
ATGCTCTTCT TCAGATCAGG TCTGTTTGTT GTGGGACCAG AGTCTGCAGG AGCCCACCCC 
GGCCCAGCCT GCTACAGGAA AGGAGGGCCA CTTACTGTGA CTGATGCCAA CCTCTGCCTG 
GGCCGCCTCC TCCCTGACTA CTTTCCCCGG ATCTTTGGCC CGTCGGAAGA CCAGCCGCTC 
TCTCGGGAAG ACACGCTGCA CCAGTTTCAG AAGTTGGCTG CTGATATTAA CCAGTTCCAG 
GCTGGGAGAC CCGGTTCTGC TGCTTCACTG AGTGTGGAGG AAGTTGCTAT GGGGTTCATC 
AGAGTGGCCA ATGAAGCCAT GTGCCGGCCA ATCAGATCTC TCACTCAGGC TAAGGGCCAC 
GACACTTCGC GCCACGTGCT GGCCTGCTTT GGGGGTGCCG GGGGGCAGCA TGCTTGTGCC 
ATCGCTCGCT CACTGGGCAT GAAAACTGTG TTTATCCACA AGTACGGCGG CGTCCTGTCT 
GCGTTTGGGA TGGCATTGGC CGATGTGGTG CACGAGGCGC AGGAGCCCTG CTCTATGGGG 
TACAGCCCCG AATCATTCTC CCTGCTGGAG GGACGCATCG CAGCCTTGGA GAGGCGCTGC 
ATAGAGGCCC TGCAGGAACA AGGCTTCCAC AGGTCTCAGA TTGACACGGA GCCATTCCTG 
CACCTGCGCT ATGAGAGAAC CGACTGCGCC CTCATGTGCT CCAACCGGGG GTACCCAGCC 
GCCCAGGACT CCTGCCTCGT GGGCAACTTC AAACTGGCCT TCACTGACAG GTACCAGAAG 
GAGTTTGGAT TCACCATCCC CTCGCGCGCC ATTGTGGTGG ATGACATCCG AGTGCGAGGG 
ACGGGCAGCA CCGGAATCCG CTGCGAGACT GCCGTTCAGC CGAGTGGGCA GCCTGCCCGA 
GTGGAGGCAG TCGCCAGATG TTACTTTGAG GACGGACACC ATGACACAAA CGTGTACCTC 
CTGCAGGACC TCGCCCCTGA CCATTCCGTT ACGGGACCCG CCATTATCAT CGACAAGAAC 
AGTACCATCC TGGTGGAACC CGACTGCACG GCCTCTATAA CCGAATACGG AGATGTCCGG 
ATCTCCATTG GGAGCGGAAG GCAGAGGGGG GTCGGCACGG AGTTGGACAC AATACAGCTG 
TCCATCTTCT CCCATCGGTT CATGAGCATT GCAGAGCAGA TGGGCCGTAT CCTGCAGAGA 
ACCGCCATTT CCACGAACAT CAAAGAGCGA CTAGACTTCT CCTGTGCTCT CTTTGGCCCT 
GATGGGGGCT TAGTGTCTAA CGCTCCCCAT ATACCTGTCC ATCTGGGTGC CATGCAGGAG 
ACAGTCCAGT TCCAGATCAG GAACCTGAAG GATGATCTGA AGGAAGGGGA TGTGCTACTG 
AGTAATCACC CCTGTGCCGG GGGCAGCCAC CTCCCAGACC TTACGGTTAT TACCCCTGTG 
TTCCGGGTGG AGGCCCCACG CCCAGTGTTT TTTGTGGCAT CCAGGGGCCA CCACGCCGAC 
ATTGGGGGGA TCACACCCGG CTCCATGCCA CCCCACTCCA AGACCCTGCG GGACGAGGGG 
GCAGTGTTCA CCTCATTCAA ACTGGTGCGA GGGGGGGAAT TCCAGGAGGA AGCGGTTACT 
GAGGCTCTTA TGGCTCCTGG TCTGATCCCG GGCAGCAGCG GGACGCGCAA TCTCCATGAC 
AACCTGTCCG ACCTGCGGGC GCAGGTAGCG GCCAATCAGA AAGGCATCCA GCTGGTGAAT 
GAGCTGATCG ATGTGTATGG GCTGGCCGTG GTACAGGCCT ACATGGCGCA CATTCAGGCC 
AATGCCGAGG TGGCTGTGCG GAACATGCTG AGAGACTTTG CTGAGCGCTG GGAGAGCCAC 
AAGGGGGCGC TAGAGGTGGA GTCTGTGGAT TACATGGACG ACGGTTCCCC AATTAAACTG 
AAAGTGCGGA TAAACAAGCA GGAGGGCGGC GCTCTCTTTG ATTTCACTGG ATCCGGATAT 
GAGGTGTTTG GGAACTGTAA CGCCCCGCGC GCCATTACCC TCTCTGCTCT TATTTATTGC 
CTCCGCTGCA TGGTGGGCCA AGATATCCCT CTTAATCAGG GCTGCCTGAC CCCCATCCAG 
GTTATCATTC CCAAAGGATC CATCCTGGAC CCCTCTCCTG ACGCGGCTGT GGTCGGGGGT 
AATGTGCTCA CATCGCAAAG GATCGTCGAT GTCATCTTCA AGGCCTTTGA AGTCTGTGCG 
GCATCTCAGG GCTGTATGAA CAATGTAACC TTTGGCAACA AGACTACGGG TTACTATGAG 
ACAGTGGCTG GCGGGGCAGG GGCGGGACCT CACTGGCACG GTCGGAGCGG CATCCACACT 
CACATGACCA ACACCCGCAT CACTGACCCA GAAATCTTGG AGAAACGATA CCCCGTGGTG 
CTGAAGCGCT TTGAGCTGTC CCCTGGTACC GGCGGCGCAG GGAAGTTCCA TGGAGGGGAT 
GGAGTGATCC GGGAGCTGCT GTTTAGGGAA GATATTGTTT TATCGGTGCT GACGGAGCGG 
CGAGTGTTCA GACCCTACGG GTTACAGGGG GGATGTAGTG GGGCCCCCGG GCTCAACCTC 
CTGCGCAGGA AGGATGGAAC AACCATTAAC CTCGGGGGCA AAACGTCAGT GAATGTGGAG 
CCGGGGGACA CGTTCACCCT TCACACCCCA GGAGGTGGGG GGTTTGGGGT TCAGGAGGGG 
AACCAAAGTA CCCCGGTACC CGTAAAGGTT CTCAGTCGCA GCTTTGCCGA GCGCGGGAGC 
GTGTTCGAGT ACCGCAAAGC TCAGGAAACT GTATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012809376.1
CDS	123..3998
Translation	MADKFQFAIDRGGTFTDVFARCPGGKVRVMKLLSEDPANYQDAPTEGIRRILEEETGCSFPRDQPLDTGRIEWIRMGTTVATNALLERKGERIALLITKGFRHLLHIGNQARPKIFDLEIQMPEVLYEEVIEVEERVVLTQGGCCLPKGEPTAPPVGSTGECLEVWEPLNLNRLEMDLRNVLARGIRSLAVVLMHSYTWSSHEHQIGELARSLGFTHVSLSSEVMPMIRIVPRGYTACADAYLTPCIKRYLHGFCRGFKNQLKDLQVLFMRSDGGLTPMQNFSGSRAILSGPAGGVVGYAITTYHRDHPQPVIGFDMGGTSTDVSRYAGEYEHVFEATTAGISIQAPQLDVNTVAAGGGSMLFFRSGLFVVGPESAGAHPGPACYRKGGPLTVTDANLCLGRLLPDYFPRIFGPSEDQPLSREDTLHQFQKLAADINQFQAGRPGSAASLSVEEVAMGFIRVANEAMCRPIRSLTQAKGHDTSRHVLACFGGAGGQHACAIARSLGMKTVFIHKYGGVLSAFGMALADVVHEAQEPCSMGYSPESFSLLEGRIAALERRCIEALQEQGFHRSQIDTEPFLHLRYERTDCALMCSNRGYPAAQDSCLVGNFKLAFTDRYQKEFGFTIPSRAIVVDDIRVRGTGSTGIRCETAVQPSGQPARVEAVARCYFEDGHHDTNVYLLQDLAPDHSVTGPAIIIDKNSTILVEPDCTASITEYGDVRISIGSGRQRGVGTELDTIQLSIFSHRFMSIAEQMGRILQRTAISTNIKERLDFSCALFGPDGGLVSNAPHIPVHLGAMQETVQFQIRNLKDDLKEGDVLLSNHPCAGGSHLPDLTVITPVFRVEAPRPVFFVASRGHHADIGGITPGSMPPHSKTLRDEGAVFTSFKLVRGGEFQEEAVTEALMAPGLIPGSSGTRNLHDNLSDLRAQVAANQKGIQLVNELIDVYGLAVVQAYMAHIQANAEVAVRNMLRDFAERWESHKGALEVESVDYMDDGSPIKLKVRINKQEGGALFDFTGSGYEVFGNCNAPRAITLSALIYCLRCMVGQDIPLNQGCLTPIQVIIPKGSILDPSPDAAVVGGNVLTSQRIVDVIFKAFEVCAASQGCMNNVTFGNKTTGYYETVAGGAGAGPHWHGRSGIHTHMTNTRITDPEILEKRYPVVLKRFELSPGTGGAGKFHGGDGVIRELLFREDIVLSVLTERRVFRPYGLQGGCSGAPGLNLLRRKDGTTINLGGKTSVNVEPGDTFTLHTPGGGGFGVQEGNQSTPVPVKVLSRSFAERGSVFEYRKAQETV

Target ORF information:

RefSeq Version	XM_012953922.3
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis 5-oxoprolinase, ATP-hydrolysing (oplah), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012953922.3

ORF Insert Sequence:

ATGGCGGATA AGTTCCAGTT CGCTATAGAC CGGGGGGGCA CATTCACGGA TGTGTTTGCG 
AGATGCCCCG GTGGGAAAGT TCGTGTTATG AAACTGCTGT CCGAGGACCC AGCGAATTAC 
CAGGATGCCC CCACAGAGGG GATCAGAAGG ATACTGGAGG AGGAGACCGG ATGTTCATTC 
CCACGGGACC AACCACTGGA CACAGGGAGG ATCGAGTGGA TCCGTATGGG CACCACTGTG 
GCCACCAATG CCCTGCTGGA AAGGAAGGGG GAGAGAATTG CTCTGCTCAT CACCAAGGGC 
TTCCGGCACC TTCTGCACAT TGGGAACCAA GCCCGGCCCA AGATCTTTGA CTTGGAGATC 
CAGATGCCCG AGGTGCTGTA CGAGGAAGTG ATAGAGGTGG AGGAGAGGGT GGTACTGACC 
CAGGGAGGCT GCTGTCTCCC AAAGGGCGAA CCCACGGCGC CCCCTGTAGG TTCTACAGGA 
GAGTGTCTGG AGGTGTGGGA GCCGCTCAAT CTGAACCGCC TGGAGATGGA TCTTCGGAAT 
GTACTGGCCC GTGGGATCAG GAGCCTGGCT GTGGTCCTTA TGCATTCTTA CACGTGGTCT 
TCCCATGAGC ACCAAATAGG GGAACTGGCA CGCTCCTTGG GGTTCACCCA TGTGTCTCTC 
TCGTCAGAGG TGATGCCAAT GATCCGCATT GTGCCCCGGG GTTACACGGC CTGCGCTGAT 
GCCTACCTCA CCCCCTGCAT AAAGAGGTAT CTCCACGGCT TCTGCCGGGG CTTCAAGAAC 
CAACTGAAGG ACTTGCAGGT CTTATTCATG CGCTCTGATG GAGGCCTGAC CCCTATGCAG 
AACTTTAGTG GCTCCCGTGC CATTCTCTCG GGCCCCGCGG GAGGGGTTGT GGGTTATGCC 
ATCACCACAT ATCACCGCGA CCACCCGCAG CCGGTCATTG GCTTTGATAT GGGAGGGACC 
TCCACAGACG TGAGCAGATA CGCCGGGGAG TATGAACATG TATTCGAAGC CACGACGGCG 
GGAATCAGCA TCCAGGCCCC ACAGCTCGAT GTCAATACAG TGGCAGCCGG AGGGGGCTCT 
ATGCTCTTCT TCAGATCAGG TCTGTTTGTT GTGGGACCAG AGTCTGCAGG AGCCCACCCC 
GGCCCAGCCT GCTACAGGAA AGGAGGGCCA CTTACTGTGA CTGATGCCAA CCTCTGCCTG 
GGCCGCCTCC TCCCTGACTA CTTTCCCCGG ATCTTTGGCC CGTCGGAAGA CCAGCCGCTC 
TCTCGGGAAG ACACGCTGCA CCAGTTTCAG AAGTTGGCTG CTGATATTAA CCAGTTCCAG 
GCTGGGAGAC CCGGTTCTGC TGCTTCACTG AGTGTGGAGG AAGTTGCTAT GGGGTTCATC 
AGAGTGGCCA ATGAAGCCAT GTGCCGGCCA ATCAGATCTC TCACTCAGGC TAAGGGCCAC 
GACACTTCGC GCCACGTGCT GGCCTGCTTT GGGGGTGCCG GGGGGCAGCA TGCTTGTGCC 
ATCGCTCGCT CACTGGGCAT GAAAACTGTG TTTATCCACA AGTACGGCGG CGTCCTGTCT 
GCGTTTGGGA TGGCATTGGC CGATGTGGTG CACGAGGCGC AGGAGCCCTG CTCTATGGGG 
TACAGCCCCG AATCATTCTC CCTGCTGGAG GGACGCATCG CAGCCTTGGA GAGGCGCTGC 
ATAGAGGCCC TGCAGGAACA AGGCTTCCAC AGGTCTCAGA TTGACACGGA GCCATTCCTG 
CACCTGCGCT ATGAGAGAAC CGACTGCGCC CTCATGTGCT CCAACCGGGG GTACCCAGCC 
GCCCAGGACT CCTGCCTCGT GGGCAACTTC AAACTGGCCT TCACTGACAG GTACCAGAAG 
GAGTTTGGAT TCACCATCCC CTCGCGCGCC ATTGTGGTGG ATGACATCCG AGTGCGAGGG 
ACGGGCAGCA CCGGAATCCG CTGCGAGACT GCCGTTCAGC CGAGTGGGCA GCCTGCCCGA 
GTGGAGGCAG TCGCCAGATG TTACTTTGAG GACGGACACC ATGACACAAA CGTGTACCTC 
CTGCAGGACC TCGCCCCTGA CCATTCCGTT ACGGGACCCG CCATTATCAT CGACAAGAAC 
AGTACCATCC TGGTGGAACC CGACTGCACG GCCTCTATAA CCGAATACGG AGATGTCCGG 
ATCTCCATTG GGAGCGGAAG GCAGAGGGGG GTCGGCACGG AGTTGGACAC AATACAGCTG 
TCCATCTTCT CCCATCGGTT CATGAGCATT GCAGAGCAGA TGGGCCGTAT CCTGCAGAGA 
ACCGCCATTT CCACGAACAT CAAAGAGCGA CTAGACTTCT CCTGTGCTCT CTTTGGCCCT 
GATGGGGGCT TAGTGTCTAA CGCTCCCCAT ATACCTGTCC ATCTGGGTGC CATGCAGGAG 
ACAGTCCAGT TCCAGATCAG GAACCTGAAG GATGATCTGA AGGAAGGGGA TGTGCTACTG 
AGTAATCACC CCTGTGCCGG GGGCAGCCAC CTCCCAGACC TTACGGTTAT TACCCCTGTG 
TTCCGGGTGG AGGCCCCACG CCCAGTGTTT TTTGTGGCAT CCAGGGGCCA CCACGCCGAC 
ATTGGGGGGA TCACACCCGG CTCCATGCCA CCCCACTCCA AGACCCTGCG GGACGAGGGG 
GCAGTGTTCA CCTCATTCAA ACTGGTGCGA GGGGGGGAAT TCCAGGAGGA AGCGGTTACT 
GAGGCTCTTA TGGCTCCTGG TCTGATCCCG GGCAGCAGCG GGACGCGCAA TCTCCATGAC 
AACCTGTCCG ACCTGCGGGC GCAGGTAGCG GCCAATCAGA AAGGCATCCA GCTGGTGAAT 
GAGCTGATCG ATGTGTATGG GCTGGCCGTG GTACAGGCCT ACATGGCGCA CATTCAGGCC 
AATGCCGAGG TGGCTGTGCG GAACATGCTG AGAGACTTTG CTGAGCGCTG GGAGAGCCAC 
AAGGGGGCGC TAGAGGTGGA GTCTGTGGAT TACATGGACG ACGGTTCCCC AATTAAACTG 
AAAGTGCGGA TAAACAAGCA GGAGGGCGGC GCTCTCTTTG ATTTCACTGG ATCCGGATAT 
GAGGTGTTTG GGAACTGTAA CGCCCCGCGC GCCATTACCC TCTCTGCTCT TATTTATTGC 
CTCCGCTGCA TGGTGGGCCA AGATATCCCT CTTAATCAGG GCTGCCTGAC CCCCATCCAG 
GTTATCATTC CCAAAGGATC CATCCTGGAC CCCTCTCCTG ACGCGGCTGT GGTCGGGGGT 
AATGTGCTCA CATCGCAAAG GATCGTCGAT GTCATCTTCA AGGCCTTTGA AGTCTGTGCG 
GCATCTCAGG GCTGTATGAA CAATGTAACC TTTGGCAACA AGACTACGGG TTACTATGAG 
ACAGTGGCTG GCGGGGCAGG GGCGGGACCT CACTGGCACG GTCGGAGCGG CATCCACACT 
CACATGACCA ACACCCGCAT CACTGACCCA GAAATCTTGG AGAAACGATA CCCCGTGGTG 
CTGAAGCGCT TTGAGCTGTC CCCTGGTACC GGCGGCGCAG GGAAGTTCCA TGGAGGGGAT 
GGAGTGATCC GGGAGCTGCT GTTTAGGGAA GATATTGTTT TATCGGTGCT GACGGAGCGG 
CGAGTGTTCA GACCCTACGG GTTACAGGGG GGATGTAGTG GGGCCCCCGG GCTCAACCTC 
CTGCGCAGGA AGGATGGAAC AACCATTAAC CTCGGGGGCA AAACGTCAGT GAATGTGGAG 
CCGGGGGACA CGTTCACCCT TCACACCCCA GGAGGTGGGG GGTTTGGGGT TCAGGAGGGG 
AACCAAAGTA CCCCGGTACC CGTAAAGGTT CTCAGTCGCA GCTTTGCCGA GCGCGGGAGC 
GTGTTCGAGT ACCGCAAAGC TCAGGAAACT GTATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

oplah ( XM_012953922.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012809376.1 Xenopus tropicalis 5-oxoprolinase (ATP-hydrolysing) (oplah), transcript variant X1, mRNA.

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CloneID	OXa29703
Clone ID Related Accession (Same CDS sequence)	XM_012953922.3 , XM_012953922.2
Accession Version	XM_012953922.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3876bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1473177600000
Organism	Xenopus tropicalis(tropical clawed frog)
Product	5-oxoprolinase isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030682.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012953922.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGATA AGTTCCAGTT CGCTATAGAC CGGGGGGGCA CATTCACGGA TGTGTTTGCG 
AGATGCCCCG GTGGGAAAGT TCGTGTTATG AAACTGCTGT CCGAGGACCC AGCGAATTAC 
CAGGATGCCC CCACAGAGGG GATCAGAAGG ATACTGGAGG AGGAGACCGG ATGTTCATTC 
CCACGGGACC AACCACTGGA CACAGGGAGG ATCGAGTGGA TCCGTATGGG CACCACTGTG 
GCCACCAATG CCCTGCTGGA AAGGAAGGGG GAGAGAATTG CTCTGCTCAT CACCAAGGGC 
TTCCGGCACC TTCTGCACAT TGGGAACCAA GCCCGGCCCA AGATCTTTGA CTTGGAGATC 
CAGATGCCCG AGGTGCTGTA CGAGGAAGTG ATAGAGGTGG AGGAGAGGGT GGTACTGACC 
CAGGGAGGCT GCTGTCTCCC AAAGGGCGAA CCCACGGCGC CCCCTGTAGG TTCTACAGGA 
GAGTGTCTGG AGGTGTGGGA GCCGCTCAAT CTGAACCGCC TGGAGATGGA TCTTCGGAAT 
GTACTGGCCC GTGGGATCAG GAGCCTGGCT GTGGTCCTTA TGCATTCTTA CACGTGGTCT 
TCCCATGAGC ACCAAATAGG GGAACTGGCA CGCTCCTTGG GGTTCACCCA TGTGTCTCTC 
TCGTCAGAGG TGATGCCAAT GATCCGCATT GTGCCCCGGG GTTACACGGC CTGCGCTGAT 
GCCTACCTCA CCCCCTGCAT AAAGAGGTAT CTCCACGGCT TCTGCCGGGG CTTCAAGAAC 
CAACTGAAGG ACTTGCAGGT CTTATTCATG CGCTCTGATG GAGGCCTGAC CCCTATGCAG 
AACTTTAGTG GCTCCCGTGC CATTCTCTCG GGCCCCGCGG GAGGGGTTGT GGGTTATGCC 
ATCACCACAT ATCACCGCGA CCACCCGCAG CCGGTCATTG GCTTTGATAT GGGAGGGACC 
TCCACAGACG TGAGCAGATA CGCCGGGGAG TATGAACATG TATTCGAAGC CACGACGGCG 
GGAATCAGCA TCCAGGCCCC ACAGCTCGAT GTCAATACAG TGGCAGCCGG AGGGGGCTCT 
ATGCTCTTCT TCAGATCAGG TCTGTTTGTT GTGGGACCAG AGTCTGCAGG AGCCCACCCC 
GGCCCAGCCT GCTACAGGAA AGGAGGGCCA CTTACTGTGA CTGATGCCAA CCTCTGCCTG 
GGCCGCCTCC TCCCTGACTA CTTTCCCCGG ATCTTTGGCC CGTCGGAAGA CCAGCCGCTC 
TCTCGGGAAG ACACGCTGCA CCAGTTTCAG AAGTTGGCTG CTGATATTAA CCAGTTCCAG 
GCTGGGAGAC CCGGTTCTGC TGCTTCACTG AGTGTGGAGG AAGTTGCTAT GGGGTTCATC 
AGAGTGGCCA ATGAAGCCAT GTGCCGGCCA ATCAGATCTC TCACTCAGGC TAAGGGCCAC 
GACACTTCGC GCCACGTGCT GGCCTGCTTT GGGGGTGCCG GGGGGCAGCA TGCTTGTGCC 
ATCGCTCGCT CACTGGGCAT GAAAACTGTG TTTATCCACA AGTACGGCGG CGTCCTGTCT 
GCGTTTGGGA TGGCATTGGC CGATGTGGTG CACGAGGCGC AGGAGCCCTG CTCTATGGGG 
TACAGCCCCG AATCATTCTC CCTGCTGGAG GGACGCATCG CAGCCTTGGA GAGGCGCTGC 
ATAGAGGCCC TGCAGGAACA AGGCTTCCAC AGGTCTCAGA TTGACACGGA GCCATTCCTG 
CACCTGCGCT ATGAGAGAAC CGACTGCGCC CTCATGTGCT CCAACCGGGG GTACCCAGCC 
GCCCAGGACT CCTGCCTCGT GGGCAACTTC AAACTGGCCT TCACTGACAG GTACCAGAAG 
GAGTTTGGAT TCACCATCCC CTCGCGCGCC ATTGTGGTGG ATGACATCCG AGTGCGAGGG 
ACGGGCAGCA CCGGAATCCG CTGCGAGACT GCCGTTCAGC CGAGTGGGCA GCCTGCCCGA 
GTGGAGGCAG TCGCCAGATG TTACTTTGAG GACGGACACC ATGACACAAA CGTGTACCTC 
CTGCAGGACC TCGCCCCTGA CCATTCCGTT ACGGGACCCG CCATTATCAT CGACAAGAAC 
AGTACCATCC TGGTGGAACC CGACTGCACG GCCTCTATAA CCGAATACGG AGATGTCCGG 
ATCTCCATTG GGAGCGGAAG GCAGAGGGGG GTCGGCACGG AGTTGGACAC AATACAGCTG 
TCCATCTTCT CCCATCGGTT CATGAGCATT GCAGAGCAGA TGGGCCGTAT CCTGCAGAGA 
ACCGCCATTT CCACGAACAT CAAAGAGCGA CTAGACTTCT CCTGTGCTCT CTTTGGCCCT 
GATGGGGGCT TAGTGTCTAA CGCTCCCCAT ATACCTGTCC ATCTGGGTGC CATGCAGGAG 
ACAGTCCAGT TCCAGATCAG GAACCTGAAG GATGATCTGA AGGAAGGGGA TGTGCTACTG 
AGTAATCACC CCTGTGCCGG GGGCAGCCAC CTCCCAGACC TTACGGTTAT TACCCCTGTG 
TTCCGGGTGG AGGCCCCACG CCCAGTGTTT TTTGTGGCAT CCAGGGGCCA CCACGCCGAC 
ATTGGGGGGA TCACACCCGG CTCCATGCCA CCCCACTCCA AGACCCTGCG GGACGAGGGG 
GCAGTGTTCA CCTCATTCAA ACTGGTGCGA GGGGGGGAAT TCCAGGAGGA AGCGGTTACT 
GAGGCTCTTA TGGCTCCTGG TCTGATCCCG GGCAGCAGCG GGACGCGCAA TCTCCATGAC 
AACCTGTCCG ACCTGCGGGC GCAGGTAGCG GCCAATCAGA AAGGCATCCA GCTGGTGAAT 
GAGCTGATCG ATGTGTATGG GCTGGCCGTG GTACAGGCCT ACATGGCGCA CATTCAGGCC 
AATGCCGAGG TGGCTGTGCG GAACATGCTG AGAGACTTTG CTGAGCGCTG GGAGAGCCAC 
AAGGGGGCGC TAGAGGTGGA GTCTGTGGAT TACATGGACG ACGGTTCCCC AATTAAACTG 
AAAGTGCGGA TAAACAAGCA GGAGGGCGGC GCTCTCTTTG ATTTCACTGG ATCCGGATAT 
GAGGTGTTTG GGAACTGTAA CGCCCCGCGC GCCATTACCC TCTCTGCTCT TATTTATTGC 
CTCCGCTGCA TGGTGGGCCA AGATATCCCT CTTAATCAGG GCTGCCTGAC CCCCATCCAG 
GTTATCATTC CCAAAGGATC CATCCTGGAC CCCTCTCCTG ACGCGGCTGT GGTCGGGGGT 
AATGTGCTCA CATCGCAAAG GATCGTCGAT GTCATCTTCA AGGCCTTTGA AGTCTGTGCG 
GCATCTCAGG GCTGTATGAA CAATGTAACC TTTGGCAACA AGACTACGGG TTACTATGAG 
ACAGTGGCTG GCGGGGCAGG GGCGGGACCT CACTGGCACG GTCGGAGCGG CATCCACACT 
CACATGACCA ACACCCGCAT CACTGACCCA GAAATCTTGG AGAAACGATA CCCCGTGGTG 
CTGAAGCGCT TTGAGCTGTC CCCTGGTACC GGCGGCGCAG GGAAGTTCCA TGGAGGGGAT 
GGAGTGATCC GGGAGCTGCT GTTTAGGGAA GATATTGTTT TATCGGTGCT GACGGAGCGG 
CGAGTGTTCA GACCCTACGG GTTACAGGGG GGATGTAGTG GGGCCCCCGG GCTCAACCTC 
CTGCGCAGGA AGGATGGAAC AACCATTAAC CTCGGGGGCA AAACGTCAGT GAATGTGGAG 
CCGGGGGACA CGTTCACCCT TCACACCCCA GGAGGTGGGG GGTTTGGGGT TCAGGAGGGG 
AACCAAAGTA CCCCGGTACC CGTAAAGGTT CTCAGTCGCA GCTTTGCCGA GCGCGGGAGC 
GTGTTCGAGT ACCGCAAAGC TCAGGAAACT GTATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012809376.1
CDS	125..4000
Translation	MADKFQFAIDRGGTFTDVFARCPGGKVRVMKLLSEDPANYQDAPTEGIRRILEEETGCSFPRDQPLDTGRIEWIRMGTTVATNALLERKGERIALLITKGFRHLLHIGNQARPKIFDLEIQMPEVLYEEVIEVEERVVLTQGGCCLPKGEPTAPPVGSTGECLEVWEPLNLNRLEMDLRNVLARGIRSLAVVLMHSYTWSSHEHQIGELARSLGFTHVSLSSEVMPMIRIVPRGYTACADAYLTPCIKRYLHGFCRGFKNQLKDLQVLFMRSDGGLTPMQNFSGSRAILSGPAGGVVGYAITTYHRDHPQPVIGFDMGGTSTDVSRYAGEYEHVFEATTAGISIQAPQLDVNTVAAGGGSMLFFRSGLFVVGPESAGAHPGPACYRKGGPLTVTDANLCLGRLLPDYFPRIFGPSEDQPLSREDTLHQFQKLAADINQFQAGRPGSAASLSVEEVAMGFIRVANEAMCRPIRSLTQAKGHDTSRHVLACFGGAGGQHACAIARSLGMKTVFIHKYGGVLSAFGMALADVVHEAQEPCSMGYSPESFSLLEGRIAALERRCIEALQEQGFHRSQIDTEPFLHLRYERTDCALMCSNRGYPAAQDSCLVGNFKLAFTDRYQKEFGFTIPSRAIVVDDIRVRGTGSTGIRCETAVQPSGQPARVEAVARCYFEDGHHDTNVYLLQDLAPDHSVTGPAIIIDKNSTILVEPDCTASITEYGDVRISIGSGRQRGVGTELDTIQLSIFSHRFMSIAEQMGRILQRTAISTNIKERLDFSCALFGPDGGLVSNAPHIPVHLGAMQETVQFQIRNLKDDLKEGDVLLSNHPCAGGSHLPDLTVITPVFRVEAPRPVFFVASRGHHADIGGITPGSMPPHSKTLRDEGAVFTSFKLVRGGEFQEEAVTEALMAPGLIPGSSGTRNLHDNLSDLRAQVAANQKGIQLVNELIDVYGLAVVQAYMAHIQANAEVAVRNMLRDFAERWESHKGALEVESVDYMDDGSPIKLKVRINKQEGGALFDFTGSGYEVFGNCNAPRAITLSALIYCLRCMVGQDIPLNQGCLTPIQVIIPKGSILDPSPDAAVVGGNVLTSQRIVDVIFKAFEVCAASQGCMNNVTFGNKTTGYYETVAGGAGAGPHWHGRSGIHTHMTNTRITDPEILEKRYPVVLKRFELSPGTGGAGKFHGGDGVIRELLFREDIVLSVLTERRVFRPYGLQGGCSGAPGLNLLRRKDGTTINLGGKTSVNVEPGDTFTLHTPGGGGFGVQEGNQSTPVPVKVLSRSFAERGSVFEYRKAQETV

Target ORF information:

RefSeq Version	XM_012953922.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis 5-oxoprolinase (ATP-hydrolysing) (oplah), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012953922.2

ORF Insert Sequence:

ATGGCGGATA AGTTCCAGTT CGCTATAGAC CGGGGGGGCA CATTCACGGA TGTGTTTGCG 
AGATGCCCCG GTGGGAAAGT TCGTGTTATG AAACTGCTGT CCGAGGACCC AGCGAATTAC 
CAGGATGCCC CCACAGAGGG GATCAGAAGG ATACTGGAGG AGGAGACCGG ATGTTCATTC 
CCACGGGACC AACCACTGGA CACAGGGAGG ATCGAGTGGA TCCGTATGGG CACCACTGTG 
GCCACCAATG CCCTGCTGGA AAGGAAGGGG GAGAGAATTG CTCTGCTCAT CACCAAGGGC 
TTCCGGCACC TTCTGCACAT TGGGAACCAA GCCCGGCCCA AGATCTTTGA CTTGGAGATC 
CAGATGCCCG AGGTGCTGTA CGAGGAAGTG ATAGAGGTGG AGGAGAGGGT GGTACTGACC 
CAGGGAGGCT GCTGTCTCCC AAAGGGCGAA CCCACGGCGC CCCCTGTAGG TTCTACAGGA 
GAGTGTCTGG AGGTGTGGGA GCCGCTCAAT CTGAACCGCC TGGAGATGGA TCTTCGGAAT 
GTACTGGCCC GTGGGATCAG GAGCCTGGCT GTGGTCCTTA TGCATTCTTA CACGTGGTCT 
TCCCATGAGC ACCAAATAGG GGAACTGGCA CGCTCCTTGG GGTTCACCCA TGTGTCTCTC 
TCGTCAGAGG TGATGCCAAT GATCCGCATT GTGCCCCGGG GTTACACGGC CTGCGCTGAT 
GCCTACCTCA CCCCCTGCAT AAAGAGGTAT CTCCACGGCT TCTGCCGGGG CTTCAAGAAC 
CAACTGAAGG ACTTGCAGGT CTTATTCATG CGCTCTGATG GAGGCCTGAC CCCTATGCAG 
AACTTTAGTG GCTCCCGTGC CATTCTCTCG GGCCCCGCGG GAGGGGTTGT GGGTTATGCC 
ATCACCACAT ATCACCGCGA CCACCCGCAG CCGGTCATTG GCTTTGATAT GGGAGGGACC 
TCCACAGACG TGAGCAGATA CGCCGGGGAG TATGAACATG TATTCGAAGC CACGACGGCG 
GGAATCAGCA TCCAGGCCCC ACAGCTCGAT GTCAATACAG TGGCAGCCGG AGGGGGCTCT 
ATGCTCTTCT TCAGATCAGG TCTGTTTGTT GTGGGACCAG AGTCTGCAGG AGCCCACCCC 
GGCCCAGCCT GCTACAGGAA AGGAGGGCCA CTTACTGTGA CTGATGCCAA CCTCTGCCTG 
GGCCGCCTCC TCCCTGACTA CTTTCCCCGG ATCTTTGGCC CGTCGGAAGA CCAGCCGCTC 
TCTCGGGAAG ACACGCTGCA CCAGTTTCAG AAGTTGGCTG CTGATATTAA CCAGTTCCAG 
GCTGGGAGAC CCGGTTCTGC TGCTTCACTG AGTGTGGAGG AAGTTGCTAT GGGGTTCATC 
AGAGTGGCCA ATGAAGCCAT GTGCCGGCCA ATCAGATCTC TCACTCAGGC TAAGGGCCAC 
GACACTTCGC GCCACGTGCT GGCCTGCTTT GGGGGTGCCG GGGGGCAGCA TGCTTGTGCC 
ATCGCTCGCT CACTGGGCAT GAAAACTGTG TTTATCCACA AGTACGGCGG CGTCCTGTCT 
GCGTTTGGGA TGGCATTGGC CGATGTGGTG CACGAGGCGC AGGAGCCCTG CTCTATGGGG 
TACAGCCCCG AATCATTCTC CCTGCTGGAG GGACGCATCG CAGCCTTGGA GAGGCGCTGC 
ATAGAGGCCC TGCAGGAACA AGGCTTCCAC AGGTCTCAGA TTGACACGGA GCCATTCCTG 
CACCTGCGCT ATGAGAGAAC CGACTGCGCC CTCATGTGCT CCAACCGGGG GTACCCAGCC 
GCCCAGGACT CCTGCCTCGT GGGCAACTTC AAACTGGCCT TCACTGACAG GTACCAGAAG 
GAGTTTGGAT TCACCATCCC CTCGCGCGCC ATTGTGGTGG ATGACATCCG AGTGCGAGGG 
ACGGGCAGCA CCGGAATCCG CTGCGAGACT GCCGTTCAGC CGAGTGGGCA GCCTGCCCGA 
GTGGAGGCAG TCGCCAGATG TTACTTTGAG GACGGACACC ATGACACAAA CGTGTACCTC 
CTGCAGGACC TCGCCCCTGA CCATTCCGTT ACGGGACCCG CCATTATCAT CGACAAGAAC 
AGTACCATCC TGGTGGAACC CGACTGCACG GCCTCTATAA CCGAATACGG AGATGTCCGG 
ATCTCCATTG GGAGCGGAAG GCAGAGGGGG GTCGGCACGG AGTTGGACAC AATACAGCTG 
TCCATCTTCT CCCATCGGTT CATGAGCATT GCAGAGCAGA TGGGCCGTAT CCTGCAGAGA 
ACCGCCATTT CCACGAACAT CAAAGAGCGA CTAGACTTCT CCTGTGCTCT CTTTGGCCCT 
GATGGGGGCT TAGTGTCTAA CGCTCCCCAT ATACCTGTCC ATCTGGGTGC CATGCAGGAG 
ACAGTCCAGT TCCAGATCAG GAACCTGAAG GATGATCTGA AGGAAGGGGA TGTGCTACTG 
AGTAATCACC CCTGTGCCGG GGGCAGCCAC CTCCCAGACC TTACGGTTAT TACCCCTGTG 
TTCCGGGTGG AGGCCCCACG CCCAGTGTTT TTTGTGGCAT CCAGGGGCCA CCACGCCGAC 
ATTGGGGGGA TCACACCCGG CTCCATGCCA CCCCACTCCA AGACCCTGCG GGACGAGGGG 
GCAGTGTTCA CCTCATTCAA ACTGGTGCGA GGGGGGGAAT TCCAGGAGGA AGCGGTTACT 
GAGGCTCTTA TGGCTCCTGG TCTGATCCCG GGCAGCAGCG GGACGCGCAA TCTCCATGAC 
AACCTGTCCG ACCTGCGGGC GCAGGTAGCG GCCAATCAGA AAGGCATCCA GCTGGTGAAT 
GAGCTGATCG ATGTGTATGG GCTGGCCGTG GTACAGGCCT ACATGGCGCA CATTCAGGCC 
AATGCCGAGG TGGCTGTGCG GAACATGCTG AGAGACTTTG CTGAGCGCTG GGAGAGCCAC 
AAGGGGGCGC TAGAGGTGGA GTCTGTGGAT TACATGGACG ACGGTTCCCC AATTAAACTG 
AAAGTGCGGA TAAACAAGCA GGAGGGCGGC GCTCTCTTTG ATTTCACTGG ATCCGGATAT 
GAGGTGTTTG GGAACTGTAA CGCCCCGCGC GCCATTACCC TCTCTGCTCT TATTTATTGC 
CTCCGCTGCA TGGTGGGCCA AGATATCCCT CTTAATCAGG GCTGCCTGAC CCCCATCCAG 
GTTATCATTC CCAAAGGATC CATCCTGGAC CCCTCTCCTG ACGCGGCTGT GGTCGGGGGT 
AATGTGCTCA CATCGCAAAG GATCGTCGAT GTCATCTTCA AGGCCTTTGA AGTCTGTGCG 
GCATCTCAGG GCTGTATGAA CAATGTAACC TTTGGCAACA AGACTACGGG TTACTATGAG 
ACAGTGGCTG GCGGGGCAGG GGCGGGACCT CACTGGCACG GTCGGAGCGG CATCCACACT 
CACATGACCA ACACCCGCAT CACTGACCCA GAAATCTTGG AGAAACGATA CCCCGTGGTG 
CTGAAGCGCT TTGAGCTGTC CCCTGGTACC GGCGGCGCAG GGAAGTTCCA TGGAGGGGAT 
GGAGTGATCC GGGAGCTGCT GTTTAGGGAA GATATTGTTT TATCGGTGCT GACGGAGCGG 
CGAGTGTTCA GACCCTACGG GTTACAGGGG GGATGTAGTG GGGCCCCCGG GCTCAACCTC 
CTGCGCAGGA AGGATGGAAC AACCATTAAC CTCGGGGGCA AAACGTCAGT GAATGTGGAG 
CCGGGGGACA CGTTCACCCT TCACACCCCA GGAGGTGGGG GGTTTGGGGT TCAGGAGGGG 
AACCAAAGTA CCCCGGTACC CGTAAAGGTT CTCAGTCGCA GCTTTGCCGA GCGCGGGAGC 
GTGTTCGAGT ACCGCAAAGC TCAGGAAACT GTATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

oplah ( XM_012953923.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012809377.1 Xenopus tropicalis 5-oxoprolinase, ATP-hydrolysing (oplah), transcript variant X2, mRNA.

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CloneID	OXa29704
Clone ID Related Accession (Same CDS sequence)	XM_012953923.1
Accession Version	XM_012953923.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3264bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1576512000000
Organism	Xenopus tropicalis(tropical clawed frog)
Product	5-oxoprolinase isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030682.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTACTGGC CCGTGGGATC AGGAGCCTGG CTGTGGTCCT TATGCATTCT TACACAGGTG 
ATGCCAATGA TCCGCATTGT GCCCCGGGGT TACACGGCCT GCGCTGATGC CTACCTCACC 
CCCTGCATAA AGAGGTATCT CCACGGCTTC TGCCGGGGCT TCAAGAACCA ACTGAAGGAC 
TTGCAGGTCT TATTCATGCG CTCTGATGGA GGCCTGACCC CTATGCAGAA CTTTAGTGGC 
TCCCGTGCCA TTCTCTCGGG CCCCGCGGGA GGGGTTGTGG GTTATGCCAT CACCACATAT 
CACCGCGACC ACCCGCAGCC GGTCATTGGC TTTGATATGG GAGGGACCTC CACAGACGTG 
AGCAGATACG CCGGGGAGTA TGAACATGTA TTCGAAGCCA CGACGGCGGG AATCAGCATC 
CAGGCCCCAC AGCTCGATGT CAATACAGTG GCAGCCGGAG GGGGCTCTAT GCTCTTCTTC 
AGATCAGGTC TGTTTGTTGT GGGACCAGAG TCTGCAGGAG CCCACCCCGG CCCAGCCTGC 
TACAGGAAAG GAGGGCCACT TACTGTGACT GATGCCAACC TCTGCCTGGG CCGCCTCCTC 
CCTGACTACT TTCCCCGGAT CTTTGGCCCG TCGGAAGACC AGCCGCTCTC TCGGGAAGAC 
ACGCTGCACC AGTTTCAGAA GTTGGCTGCT GATATTAACC AGTTCCAGGC TGGGAGACCC 
GGTTCTGCTG CTTCACTGAG TGTGGAGGAA GTTGCTATGG GGTTCATCAG AGTGGCCAAT 
GAAGCCATGT GCCGGCCAAT CAGATCTCTC ACTCAGGCTA AGGGCCACGA CACTTCGCGC 
CACGTGCTGG CCTGCTTTGG GGGTGCCGGG GGGCAGCATG CTTGTGCCAT CGCTCGCTCA 
CTGGGCATGA AAACTGTGTT TATCCACAAG TACGGCGGCG TCCTGTCTGC GTTTGGGATG 
GCATTGGCCG ATGTGGTGCA CGAGGCGCAG GAGCCCTGCT CTATGGGGTA CAGCCCCGAA 
TCATTCTCCC TGCTGGAGGG ACGCATCGCA GCCTTGGAGA GGCGCTGCAT AGAGGCCCTG 
CAGGAACAAG GCTTCCACAG GTCTCAGATT GACACGGAGC CATTCCTGCA CCTGCGCTAT 
GAGAGAACCG ACTGCGCCCT CATGTGCTCC AACCGGGGGT ACCCAGCCGC CCAGGACTCC 
TGCCTCGTGG GCAACTTCAA ACTGGCCTTC ACTGACAGGT ACCAGAAGGA GTTTGGATTC 
ACCATCCCCT CGCGCGCCAT TGTGGTGGAT GACATCCGAG TGCGAGGGAC GGGCAGCACC 
GGAATCCGCT GCGAGACTGC CGTTCAGCCG AGTGGGCAGC CTGCCCGAGT GGAGGCAGTC 
GCCAGATGTT ACTTTGAGGA CGGACACCAT GACACAAACG TGTACCTCCT GCAGGACCTC 
GCCCCTGACC ATTCCGTTAC GGGACCCGCC ATTATCATCG ACAAGAACAG TACCATCCTG 
GTGGAACCCG ACTGCACGGC CTCTATAACC GAATACGGAG ATGTCCGGAT CTCCATTGGG 
AGCGGAAGGC AGAGGGGGGT CGGCACGGAG TTGGACACAA TACAGCTGTC CATCTTCTCC 
CATCGGTTCA TGAGCATTGC AGAGCAGATG GGCCGTATCC TGCAGAGAAC CGCCATTTCC 
ACGAACATCA AAGAGCGACT AGACTTCTCC TGTGCTCTCT TTGGCCCTGA TGGGGGCTTA 
GTGTCTAACG CTCCCCATAT ACCTGTCCAT CTGGGTGCCA TGCAGGAGAC AGTCCAGTTC 
CAGATCAGGA ACCTGAAGGA TGATCTGAAG GAAGGGGATG TGCTACTGAG TAATCACCCC 
TGTGCCGGGG GCAGCCACCT CCCAGACCTT ACGGTTATTA CCCCTGTGTT CCGGGTGGAG 
GCCCCACGCC CAGTGTTTTT TGTGGCATCC AGGGGCCACC ACGCCGACAT TGGGGGGATC 
ACACCCGGCT CCATGCCACC CCACTCCAAG ACCCTGCGGG ACGAGGGGGC AGTGTTCACC 
TCATTCAAAC TGGTGCGAGG GGGGGAATTC CAGGAGGAAG CGGTTACTGA GGCTCTTATG 
GCTCCTGGTC TGATCCCGGG CAGCAGCGGG ACGCGCAATC TCCATGACAA CCTGTCCGAC 
CTGCGGGCGC AGGTAGCGGC CAATCAGAAA GGCATCCAGC TGGTGAATGA GCTGATCGAT 
GTGTATGGGC TGGCCGTGGT ACAGGCCTAC ATGGCGCACA TTCAGGCCAA TGCCGAGGTG 
GCTGTGCGGA ACATGCTGAG AGACTTTGCT GAGCGCTGGG AGAGCCACAA GGGGGCGCTA 
GAGGTGGAGT CTGTGGATTA CATGGACGAC GGTTCCCCAA TTAAACTGAA AGTGCGGATA 
AACAAGCAGG AGGGCGGCGC TCTCTTTGAT TTCACTGGAT CCGGATATGA GGTGTTTGGG 
AACTGTAACG CCCCGCGCGC CATTACCCTC TCTGCTCTTA TTTATTGCCT CCGCTGCATG 
GTGGGCCAAG ATATCCCTCT TAATCAGGGC TGCCTGACCC CCATCCAGGT TATCATTCCC 
AAAGGATCCA TCCTGGACCC CTCTCCTGAC GCGGCTGTGG TCGGGGGTAA TGTGCTCACA 
TCGCAAAGGA TCGTCGATGT CATCTTCAAG GCCTTTGAAG TCTGTGCGGC ATCTCAGGGC 
TGTATGAACA ATGTAACCTT TGGCAACAAG ACTACGGGTT ACTATGAGAC AGTGGCTGGC 
GGGGCAGGGG CGGGACCTCA CTGGCACGGT CGGAGCGGCA TCCACACTCA CATGACCAAC 
ACCCGCATCA CTGACCCAGA AATCTTGGAG AAACGATACC CCGTGGTGCT GAAGCGCTTT 
GAGCTGTCCC CTGGTACCGG CGGCGCAGGG AAGTTCCATG GAGGGGATGG AGTGATCCGG 
GAGCTGCTGT TTAGGGAAGA TATTGTTTTA TCGGTGCTGA CGGAGCGGCG AGTGTTCAGA 
CCCTACGGGT TACAGGGGGG ATGTAGTGGG GCCCCCGGGC TCAACCTCCT GCGCAGGAAG 
GATGGAACAA CCATTAACCT CGGGGGCAAA ACGTCAGTGA ATGTGGAGCC GGGGGACACG 
TTCACCCTTC ACACCCCAGG AGGTGGGGGG TTTGGGGTTC AGGAGGGGAA CCAAAGTACC 
CCGGTACCCG TAAAGGTTCT CAGTCGCAGC TTTGCCGAGC GCGGGAGCGT GTTCGAGTAC 
CGCAAAGCTC AGGAAACTGT ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012809377.1
CDS	158..3421
Translation	MYWPVGSGAWLWSLCILTQVMPMIRIVPRGYTACADAYLTPCIKRYLHGFCRGFKNQLKDLQVLFMRSDGGLTPMQNFSGSRAILSGPAGGVVGYAITTYHRDHPQPVIGFDMGGTSTDVSRYAGEYEHVFEATTAGISIQAPQLDVNTVAAGGGSMLFFRSGLFVVGPESAGAHPGPACYRKGGPLTVTDANLCLGRLLPDYFPRIFGPSEDQPLSREDTLHQFQKLAADINQFQAGRPGSAASLSVEEVAMGFIRVANEAMCRPIRSLTQAKGHDTSRHVLACFGGAGGQHACAIARSLGMKTVFIHKYGGVLSAFGMALADVVHEAQEPCSMGYSPESFSLLEGRIAALERRCIEALQEQGFHRSQIDTEPFLHLRYERTDCALMCSNRGYPAAQDSCLVGNFKLAFTDRYQKEFGFTIPSRAIVVDDIRVRGTGSTGIRCETAVQPSGQPARVEAVARCYFEDGHHDTNVYLLQDLAPDHSVTGPAIIIDKNSTILVEPDCTASITEYGDVRISIGSGRQRGVGTELDTIQLSIFSHRFMSIAEQMGRILQRTAISTNIKERLDFSCALFGPDGGLVSNAPHIPVHLGAMQETVQFQIRNLKDDLKEGDVLLSNHPCAGGSHLPDLTVITPVFRVEAPRPVFFVASRGHHADIGGITPGSMPPHSKTLRDEGAVFTSFKLVRGGEFQEEAVTEALMAPGLIPGSSGTRNLHDNLSDLRAQVAANQKGIQLVNELIDVYGLAVVQAYMAHIQANAEVAVRNMLRDFAERWESHKGALEVESVDYMDDGSPIKLKVRINKQEGGALFDFTGSGYEVFGNCNAPRAITLSALIYCLRCMVGQDIPLNQGCLTPIQVIIPKGSILDPSPDAAVVGGNVLTSQRIVDVIFKAFEVCAASQGCMNNVTFGNKTTGYYETVAGGAGAGPHWHGRSGIHTHMTNTRITDPEILEKRYPVVLKRFELSPGTGGAGKFHGGDGVIRELLFREDIVLSVLTERRVFRPYGLQGGCSGAPGLNLLRRKDGTTINLGGKTSVNVEPGDTFTLHTPGGGGFGVQEGNQSTPVPVKVLSRSFAERGSVFEYRKAQETV

Target ORF information:

RefSeq Version	XM_012953923.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis 5-oxoprolinase, ATP-hydrolysing (oplah), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012953923.1

ORF Insert Sequence:

ATGTACTGGC CCGTGGGATC AGGAGCCTGG CTGTGGTCCT TATGCATTCT TACACAGGTG 
ATGCCAATGA TCCGCATTGT GCCCCGGGGT TACACGGCCT GCGCTGATGC CTACCTCACC 
CCCTGCATAA AGAGGTATCT CCACGGCTTC TGCCGGGGCT TCAAGAACCA ACTGAAGGAC 
TTGCAGGTCT TATTCATGCG CTCTGATGGA GGCCTGACCC CTATGCAGAA CTTTAGTGGC 
TCCCGTGCCA TTCTCTCGGG CCCCGCGGGA GGGGTTGTGG GTTATGCCAT CACCACATAT 
CACCGCGACC ACCCGCAGCC GGTCATTGGC TTTGATATGG GAGGGACCTC CACAGACGTG 
AGCAGATACG CCGGGGAGTA TGAACATGTA TTCGAAGCCA CGACGGCGGG AATCAGCATC 
CAGGCCCCAC AGCTCGATGT CAATACAGTG GCAGCCGGAG GGGGCTCTAT GCTCTTCTTC 
AGATCAGGTC TGTTTGTTGT GGGACCAGAG TCTGCAGGAG CCCACCCCGG CCCAGCCTGC 
TACAGGAAAG GAGGGCCACT TACTGTGACT GATGCCAACC TCTGCCTGGG CCGCCTCCTC 
CCTGACTACT TTCCCCGGAT CTTTGGCCCG TCGGAAGACC AGCCGCTCTC TCGGGAAGAC 
ACGCTGCACC AGTTTCAGAA GTTGGCTGCT GATATTAACC AGTTCCAGGC TGGGAGACCC 
GGTTCTGCTG CTTCACTGAG TGTGGAGGAA GTTGCTATGG GGTTCATCAG AGTGGCCAAT 
GAAGCCATGT GCCGGCCAAT CAGATCTCTC ACTCAGGCTA AGGGCCACGA CACTTCGCGC 
CACGTGCTGG CCTGCTTTGG GGGTGCCGGG GGGCAGCATG CTTGTGCCAT CGCTCGCTCA 
CTGGGCATGA AAACTGTGTT TATCCACAAG TACGGCGGCG TCCTGTCTGC GTTTGGGATG 
GCATTGGCCG ATGTGGTGCA CGAGGCGCAG GAGCCCTGCT CTATGGGGTA CAGCCCCGAA 
TCATTCTCCC TGCTGGAGGG ACGCATCGCA GCCTTGGAGA GGCGCTGCAT AGAGGCCCTG 
CAGGAACAAG GCTTCCACAG GTCTCAGATT GACACGGAGC CATTCCTGCA CCTGCGCTAT 
GAGAGAACCG ACTGCGCCCT CATGTGCTCC AACCGGGGGT ACCCAGCCGC CCAGGACTCC 
TGCCTCGTGG GCAACTTCAA ACTGGCCTTC ACTGACAGGT ACCAGAAGGA GTTTGGATTC 
ACCATCCCCT CGCGCGCCAT TGTGGTGGAT GACATCCGAG TGCGAGGGAC GGGCAGCACC 
GGAATCCGCT GCGAGACTGC CGTTCAGCCG AGTGGGCAGC CTGCCCGAGT GGAGGCAGTC 
GCCAGATGTT ACTTTGAGGA CGGACACCAT GACACAAACG TGTACCTCCT GCAGGACCTC 
GCCCCTGACC ATTCCGTTAC GGGACCCGCC ATTATCATCG ACAAGAACAG TACCATCCTG 
GTGGAACCCG ACTGCACGGC CTCTATAACC GAATACGGAG ATGTCCGGAT CTCCATTGGG 
AGCGGAAGGC AGAGGGGGGT CGGCACGGAG TTGGACACAA TACAGCTGTC CATCTTCTCC 
CATCGGTTCA TGAGCATTGC AGAGCAGATG GGCCGTATCC TGCAGAGAAC CGCCATTTCC 
ACGAACATCA AAGAGCGACT AGACTTCTCC TGTGCTCTCT TTGGCCCTGA TGGGGGCTTA 
GTGTCTAACG CTCCCCATAT ACCTGTCCAT CTGGGTGCCA TGCAGGAGAC AGTCCAGTTC 
CAGATCAGGA ACCTGAAGGA TGATCTGAAG GAAGGGGATG TGCTACTGAG TAATCACCCC 
TGTGCCGGGG GCAGCCACCT CCCAGACCTT ACGGTTATTA CCCCTGTGTT CCGGGTGGAG 
GCCCCACGCC CAGTGTTTTT TGTGGCATCC AGGGGCCACC ACGCCGACAT TGGGGGGATC 
ACACCCGGCT CCATGCCACC CCACTCCAAG ACCCTGCGGG ACGAGGGGGC AGTGTTCACC 
TCATTCAAAC TGGTGCGAGG GGGGGAATTC CAGGAGGAAG CGGTTACTGA GGCTCTTATG 
GCTCCTGGTC TGATCCCGGG CAGCAGCGGG ACGCGCAATC TCCATGACAA CCTGTCCGAC 
CTGCGGGCGC AGGTAGCGGC CAATCAGAAA GGCATCCAGC TGGTGAATGA GCTGATCGAT 
GTGTATGGGC TGGCCGTGGT ACAGGCCTAC ATGGCGCACA TTCAGGCCAA TGCCGAGGTG 
GCTGTGCGGA ACATGCTGAG AGACTTTGCT GAGCGCTGGG AGAGCCACAA GGGGGCGCTA 
GAGGTGGAGT CTGTGGATTA CATGGACGAC GGTTCCCCAA TTAAACTGAA AGTGCGGATA 
AACAAGCAGG AGGGCGGCGC TCTCTTTGAT TTCACTGGAT CCGGATATGA GGTGTTTGGG 
AACTGTAACG CCCCGCGCGC CATTACCCTC TCTGCTCTTA TTTATTGCCT CCGCTGCATG 
GTGGGCCAAG ATATCCCTCT TAATCAGGGC TGCCTGACCC CCATCCAGGT TATCATTCCC 
AAAGGATCCA TCCTGGACCC CTCTCCTGAC GCGGCTGTGG TCGGGGGTAA TGTGCTCACA 
TCGCAAAGGA TCGTCGATGT CATCTTCAAG GCCTTTGAAG TCTGTGCGGC ATCTCAGGGC 
TGTATGAACA ATGTAACCTT TGGCAACAAG ACTACGGGTT ACTATGAGAC AGTGGCTGGC 
GGGGCAGGGG CGGGACCTCA CTGGCACGGT CGGAGCGGCA TCCACACTCA CATGACCAAC 
ACCCGCATCA CTGACCCAGA AATCTTGGAG AAACGATACC CCGTGGTGCT GAAGCGCTTT 
GAGCTGTCCC CTGGTACCGG CGGCGCAGGG AAGTTCCATG GAGGGGATGG AGTGATCCGG 
GAGCTGCTGT TTAGGGAAGA TATTGTTTTA TCGGTGCTGA CGGAGCGGCG AGTGTTCAGA 
CCCTACGGGT TACAGGGGGG ATGTAGTGGG GCCCCCGGGC TCAACCTCCT GCGCAGGAAG 
GATGGAACAA CCATTAACCT CGGGGGCAAA ACGTCAGTGA ATGTGGAGCC GGGGGACACG 
TTCACCCTTC ACACCCCAGG AGGTGGGGGG TTTGGGGTTC AGGAGGGGAA CCAAAGTACC 
CCGGTACCCG TAAAGGTTCT CAGTCGCAGC TTTGCCGAGC GCGGGAGCGT GTTCGAGTAC 
CGCAAAGCTC AGGAAACTGT ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Genetic and genomic tools for Xenopus research: The NIH Xenopus initiative.
Developmental dynamics : an official publication of the American Association of Anatomists225(4)384-91(2002 Dec)
Klein SL,Strausberg RL,Wagner L,Pontius J,Clifton SW,Richardson P

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oplah cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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