smg8 cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

Gene
Clones
gRNAs

Summary

Gene Symbol	smg8
Entrez Gene ID	779744
Full Name	SMG8 nonsense mediated mRNA decay factor
Synonyms	c17orf71
General protein information	Preferred Names SMG8 nonsense mediated mRNA decay factor Names protein smg8 protein smg-8 homolog smg-8 homolog, nonsense mediated mRNA decay factor
Gene Type	protein-coding
Organism	Xenopus tropicalis(tropical clawed frog)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001078823.1	NP_001072291.1	protein smg8

Pathways from BioSystems

Homologies

Homo sapiens (human)	SMG8	NP_060619.4
Xenopus tropicalis (tropical clawed frog)	smg8	NP_001072291.1
Bos taurus (cattle)	SMG8	NP_001073755.1
Mus musculus (house mouse)	Smg8	NP_077224.1
Macaca mulatta (Rhesus monkey)	C17orf71	XP_001107695.1
Canis lupus familiaris (dog)	SMG8	XP_548236.1
Rattus norvegicus (Norway rat)	Smg8	XP_213430.2
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP010179	XP_319357.4
Pan troglodytes (chimpanzee)	SMG8	XP_523682.1
Gallus gallus (chicken)	SMG8	XP_415875.4
Danio rerio (zebrafish)	smg8	XP_695811.2
Drosophila melanogaster (fruit fly)	CG6729	NP_609449.1

Gene Ontology
Bibliography

Related articles in PubMed

Genetic and genomic tools for Xenopus research: The NIH Xenopus initiative.
Klein SL, Strausberg RL, Wagner L, Pontius J, Clifton SW, Richardson P
Developmental dynamics : an official publication of the American Association of Anatomists225(4)384-91(2002 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following smg8 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the smg8 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OXa00702	NM_001078823.1 Latest version!	Xenopus tropicalis SMG8 nonsense mediated mRNA decay factor (smg8), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

smg8 ( NM_001078823.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> NP_001072291.1 Xenopus tropicalis SMG8 nonsense mediated mRNA decay factor (smg8), mRNA.

Price & Availability↑

CloneID	OXa00702
Clone ID Related Accession (Same CDS sequence)	NM_001078823.1
Accession Version	NM_001078823.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2748bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1576857600000
Organism	Xenopus tropicalis(tropical clawed frog)
Product	protein smg8
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC121322.1. ##Evidence-Data-START## Transcript exon combination :: BC121322.1, CT025291.2 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMD00028290, SAMD00028291 [ECO:0000348] ##Evidence-Data-END##

ATGGTGGTGT GTGGGGGTCC CACAGTGTTA CGGGACCTTC TAGGGGCGGA CACGGAGCCA 
ACCTGGAAGG ATGAGGAGGT TTGCGTTGTG GGACTTTTCG GTAAGACGGC GCTGGCCCAG 
GGCAGCTGGC ACAAATGCTC TCTCATAAAC TCTCTGTGCG ACCGGCACAT TTTCCCGCTG 
TTTCACCGCG CCCCTGAGAG GCCCTCCGAG CGTAGTCTCT TCCAGACCTA TTACGAGCAG 
GAGTCCCGGG TGCTGTACGT CCTTCTGGCA GGGCTGAGTG ATACGGGCTC GCTGCTGAAG 
GCTTGTGAGG AGCTGAGCCG CGGAGTGTCC CATGCAGAGG CTCACGAGTG GTGGAAGGAT 
GAGGAGAAGT TGTACTGCAT GCACCTGCTC TATCTATTCT CAGTGTGCCA TATCCTGGTT 
CTGGTGCACC CTACCTGCTG CTTTGATATC ACCTATGAGA AACTGTTCAG GGCTTTGGAT 
AGTCTCAGGC AAAAGATGTT GCCATCACTG AAGCCAAGCT TAAAGGATTG TGCTGTGGGT 
CTGGACTGGA AACTCAATGC CAGACCTTGT CCTCCCAGGC TGCTCTTTAT CTTTCAGCTC 
AACGGAGCTC TTAAAGTAGA GCCAAAGAGT CAAGGCCCAC AAACCAACGA GAAACCCAAG 
AAGCACTCTC CAAAGAGAAG ACTGCAGCAC GCTTTGGAAG ACCAGATCTA CCGCATCTTT 
AGAAAAAGCA GAGTCCTGAC TAACCAATCA ATCAATTGTC TATTCACTGT GCCTGCTAAT 
CAGGCCTTTG TGTATATTGT GGCCGATGAA GATGAAGATC CTGTAAACAT GCTACTGGAG 
GGTTTGAGGC ACAATTGCAC TTTGAAAGAT ACAGAGTCTT TGGTACCTAT ATCTGGTCCT 
AGACGTTATC AAATGATGAG ACACACGCGC CAACTGTCTT TCACGGTTGA AAATAACACA 
AGTCTATCTG GTCAGTTGGT TGACTGCACC CTAAGAGAGT TCCTGTTCCA GCATGTGGAG 
CTGGTTCTCA CAAAAAAGGG ATTTGATGAC AGTGTAGGAA GAAATCCACA GCCATCTCAC 
TTTGAGCTCC CAACATACCA AAAGTGGGTT AGTGTGGCTT TAAAACTGTA TGAAATTATC 
ATTGAAAATA AAGATGATGA CCCCCCTGCT TTTCCTGGTG GATTCCCCCC AAAGCTTCTT 
GCTAACATGA AGGTCTTAGA AGGTTATCTG GATGCGGACA CCAAGTTCTC AGAAAACAGA 
TGCCAAAAAG CTCTACCTAT GGCACATAGC GCTTATCAGT CTAATTTGCC CCATAATTAC 
ACCACAACTG TCCATAAAAA CCAGCTTGCC CAGGCCCTAC GAGTATATAG CCAGCATGCC 
CGGGGACCTG CTTTTCATAA ATATGCCATG GTTCTTAATG AAGATTGTTA TAAGTTCTGG 
AGCAGTGGGC ATCAGCTTTG TGAAGAAAGG AGCTTGACAG ATCAACACTG TGTACACAAA 
TTTCATTTGC TGCCTAAATC TGGGGAAAAA ATTGAACCAG AGAGAAATCC ACCAATCCTC 
TTCCACAACA GTCGGGCGCG TTCCACAGGC AGCTGTAATT GTGGAAAAAA ACAGGCACCA 
CGAGAAGATC CATTTGACAT AAAAAGTGCA AACTATGATT TTTATCAGAT ACTGGAAGAA 
AAATGTTGTG GAAAGTTGGA TCACATCACC TTCCCCATAT TCCAGCCCAG CACACCTGAC 
CCAGCTCCTG CAAAAAATGA GGCTTCACCT GCTGCACATG ATGGTGAAGT TGAAGGAGAA 
AAGATGAAGG ATAAGGAGCC ACAAACTCAG GGAGAAAGTA CTAGCCTAAG TTTAGCTCTA 
AGCCTGGGCC AATCCACTGA CAGTTTGGGC ACATTCCCAG AGGGCCCAAA GGCAGGAGGG 
GACAATACAG AAGCTTCGGG CCATGGGACA GATGAGAAGG TTGAAAAAAG GCCAAGTTTA 
GTAGATCGTC AAGCCTCAAC TGTAGAATAT CTGCCTGGCA TGGTACATTT AAATTCACCA 
AAAGGACTTT TACCTAAATT CTCTAGTTGG GCTTTGGTAA AACTTGGTCC TGCTAAATCC 
TACAATTTCC ACACTGGATT AGACCATCTT GGCTTTATTC CAGGAACAAG TTACTTGATG 
CCTTGGGACA TTGTTATTCG CACTAAGCCA GAAGATGAAA GTGACCTTGA CACAAACTCT 
TGGCCAGCTC CCAACAAGTC CATCCCTGGA AAAAGAAATT CTGTAGTAAT GGGGCGGGGA 
CGTAGAAGAG ATGACATTGC AAGGGCATTT GTAGGTTTTG AGTATGAGGA TTCTAGGGGA 
CGGAGATTTA TGTGCTCAGG ACCGGACAAA ATAATGAAGG CAATCGGTAA TGGACCCAAA 
GAATCTGCAA TCAAAGCACT CAACACTGAC ATGCCATTGT ATATGCTATC ACCATCACAG 
GGCAGAGGGC TTAAACCTCA TTATGCTCAG CTTATGAGAC TGTTTGTTGT CGTCCCAGAT 
GCTCCTGTAC AAATTATATT GGCTCCTCAG GTGCAGCCGG GACCTCCTCC ATGCCCTGTC 
TTTGTCCCTG AAAAACAGGA AATCACCCTT CCTTCTGATG GACTGTGGGT TCTTAGGTTT 
CCTTTTTCTT ATGCCTCAGA GCGTGGGCCC TGTTACTCTC CCAAGGAAAA TCAGCAGCTT 
CCAAGCTACA AAGTCATGAA AGGAATACTG CGAACTGTCA CTCAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001072291.1
CDS	71..2818
Translation	MVVCGGPTVLRDLLGADTEPTWKDEEVCVVGLFGKTALAQGSWHKCSLINSLCDRHIFPLFHRAPERPSERSLFQTYYEQESRVLYVLLAGLSDTGSLLKACEELSRGVSHAEAHEWWKDEEKLYCMHLLYLFSVCHILVLVHPTCCFDITYEKLFRALDSLRQKMLPSLKPSLKDCAVGLDWKLNARPCPPRLLFIFQLNGALKVEPKSQGPQTNEKPKKHSPKRRLQHALEDQIYRIFRKSRVLTNQSINCLFTVPANQAFVYIVADEDEDPVNMLLEGLRHNCTLKDTESLVPISGPRRYQMMRHTRQLSFTVENNTSLSGQLVDCTLREFLFQHVELVLTKKGFDDSVGRNPQPSHFELPTYQKWVSVALKLYEIIIENKDDDPPAFPGGFPPKLLANMKVLEGYLDADTKFSENRCQKALPMAHSAYQSNLPHNYTTTVHKNQLAQALRVYSQHARGPAFHKYAMVLNEDCYKFWSSGHQLCEERSLTDQHCVHKFHLLPKSGEKIEPERNPPILFHNSRARSTGSCNCGKKQAPREDPFDIKSANYDFYQILEEKCCGKLDHITFPIFQPSTPDPAPAKNEASPAAHDGEVEGEKMKDKEPQTQGESTSLSLALSLGQSTDSLGTFPEGPKAGGDNTEASGHGTDEKVEKRPSLVDRQASTVEYLPGMVHLNSPKGLLPKFSSWALVKLGPAKSYNFHTGLDHLGFIPGTSYLMPWDIVIRTKPEDESDLDTNSWPAPNKSIPGKRNSVVMGRGRRRDDIARAFVGFEYEDSRGRRFMCSGPDKIMKAIGNGPKESAIKALNTDMPLYMLSPSQGRGLKPHYAQLMRLFVVVPDAPVQIILAPQVQPGPPPCPVFVPEKQEITLPSDGLWVLRFPFSYASERGPCYSPKENQQLPSYKVMKGILRTVTQ

Target ORF information:

RefSeq Version	NM_001078823.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis SMG8 nonsense mediated mRNA decay factor (smg8), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001078823.1

ORF Insert Sequence:

ATGGTGGTGT GTGGGGGTCC CACAGTGTTA CGGGACCTTC TAGGGGCGGA CACGGAGCCA 
ACCTGGAAGG ATGAGGAGGT TTGCGTTGTG GGACTTTTCG GTAAGACGGC GCTGGCCCAG 
GGCAGCTGGC ACAAATGCTC TCTCATAAAC TCTCTGTGCG ACCGGCACAT TTTCCCGCTG 
TTTCACCGCG CCCCTGAGAG GCCCTCCGAG CGTAGTCTCT TCCAGACCTA TTACGAGCAG 
GAGTCCCGGG TGCTGTACGT CCTTCTGGCA GGGCTGAGTG ATACGGGCTC GCTGCTGAAG 
GCTTGTGAGG AGCTGAGCCG CGGAGTGTCC CATGCAGAGG CTCACGAGTG GTGGAAGGAT 
GAGGAGAAGT TGTACTGCAT GCACCTGCTC TATCTATTCT CAGTGTGCCA TATCCTGGTT 
CTGGTGCACC CTACCTGCTG CTTTGATATC ACCTATGAGA AACTGTTCAG GGCTTTGGAT 
AGTCTCAGGC AAAAGATGTT GCCATCACTG AAGCCAAGCT TAAAGGATTG TGCTGTGGGT 
CTGGACTGGA AACTCAATGC CAGACCTTGT CCTCCCAGGC TGCTCTTTAT CTTTCAGCTC 
AACGGAGCTC TTAAAGTAGA GCCAAAGAGT CAAGGCCCAC AAACCAACGA GAAACCCAAG 
AAGCACTCTC CAAAGAGAAG ACTGCAGCAC GCTTTGGAAG ACCAGATCTA CCGCATCTTT 
AGAAAAAGCA GAGTCCTGAC TAACCAATCA ATCAATTGTC TATTCACTGT GCCTGCTAAT 
CAGGCCTTTG TGTATATTGT GGCCGATGAA GATGAAGATC CTGTAAACAT GCTACTGGAG 
GGTTTGAGGC ACAATTGCAC TTTGAAAGAT ACAGAGTCTT TGGTACCTAT ATCTGGTCCT 
AGACGTTATC AAATGATGAG ACACACGCGC CAACTGTCTT TCACGGTTGA AAATAACACA 
AGTCTATCTG GTCAGTTGGT TGACTGCACC CTAAGAGAGT TCCTGTTCCA GCATGTGGAG 
CTGGTTCTCA CAAAAAAGGG ATTTGATGAC AGTGTAGGAA GAAATCCACA GCCATCTCAC 
TTTGAGCTCC CAACATACCA AAAGTGGGTT AGTGTGGCTT TAAAACTGTA TGAAATTATC 
ATTGAAAATA AAGATGATGA CCCCCCTGCT TTTCCTGGTG GATTCCCCCC AAAGCTTCTT 
GCTAACATGA AGGTCTTAGA AGGTTATCTG GATGCGGACA CCAAGTTCTC AGAAAACAGA 
TGCCAAAAAG CTCTACCTAT GGCACATAGC GCTTATCAGT CTAATTTGCC CCATAATTAC 
ACCACAACTG TCCATAAAAA CCAGCTTGCC CAGGCCCTAC GAGTATATAG CCAGCATGCC 
CGGGGACCTG CTTTTCATAA ATATGCCATG GTTCTTAATG AAGATTGTTA TAAGTTCTGG 
AGCAGTGGGC ATCAGCTTTG TGAAGAAAGG AGCTTGACAG ATCAACACTG TGTACACAAA 
TTTCATTTGC TGCCTAAATC TGGGGAAAAA ATTGAACCAG AGAGAAATCC ACCAATCCTC 
TTCCACAACA GTCGGGCGCG TTCCACAGGC AGCTGTAATT GTGGAAAAAA ACAGGCACCA 
CGAGAAGATC CATTTGACAT AAAAAGTGCA AACTATGATT TTTATCAGAT ACTGGAAGAA 
AAATGTTGTG GAAAGTTGGA TCACATCACC TTCCCCATAT TCCAGCCCAG CACACCTGAC 
CCAGCTCCTG CAAAAAATGA GGCTTCACCT GCTGCACATG ATGGTGAAGT TGAAGGAGAA 
AAGATGAAGG ATAAGGAGCC ACAAACTCAG GGAGAAAGTA CTAGCCTAAG TTTAGCTCTA 
AGCCTGGGCC AATCCACTGA CAGTTTGGGC ACATTCCCAG AGGGCCCAAA GGCAGGAGGG 
GACAATACAG AAGCTTCGGG CCATGGGACA GATGAGAAGG TTGAAAAAAG GCCAAGTTTA 
GTAGATCGTC AAGCCTCAAC TGTAGAATAT CTGCCTGGCA TGGTACATTT AAATTCACCA 
AAAGGACTTT TACCTAAATT CTCTAGTTGG GCTTTGGTAA AACTTGGTCC TGCTAAATCC 
TACAATTTCC ACACTGGATT AGACCATCTT GGCTTTATTC CAGGAACAAG TTACTTGATG 
CCTTGGGACA TTGTTATTCG CACTAAGCCA GAAGATGAAA GTGACCTTGA CACAAACTCT 
TGGCCAGCTC CCAACAAGTC CATCCCTGGA AAAAGAAATT CTGTAGTAAT GGGGCGGGGA 
CGTAGAAGAG ATGACATTGC AAGGGCATTT GTAGGTTTTG AGTATGAGGA TTCTAGGGGA 
CGGAGATTTA TGTGCTCAGG ACCGGACAAA ATAATGAAGG CAATCGGTAA TGGACCCAAA 
GAATCTGCAA TCAAAGCACT CAACACTGAC ATGCCATTGT ATATGCTATC ACCATCACAG 
GGCAGAGGGC TTAAACCTCA TTATGCTCAG CTTATGAGAC TGTTTGTTGT CGTCCCAGAT 
GCTCCTGTAC AAATTATATT GGCTCCTCAG GTGCAGCCGG GACCTCCTCC ATGCCCTGTC 
TTTGTCCCTG AAAAACAGGA AATCACCCTT CCTTCTGATG GACTGTGGGT TCTTAGGTTT 
CCTTTTTCTT ATGCCTCAGA GCGTGGGCCC TGTTACTCTC CCAAGGAAAA TCAGCAGCTT 
CCAAGCTACA AAGTCATGAA AGGAATACTG CGAACTGTCA CTCAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Genetic and genomic tools for Xenopus research: The NIH Xenopus initiative.
Developmental dynamics : an official publication of the American Association of Anatomists225(4)384-91(2002 Dec)
Klein SL,Strausberg RL,Wagner L,Pontius J,Clifton SW,Richardson P

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smg8 cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

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