top2b cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

Gene
Clones
gRNAs

Summary

Gene Symbol	top2b
Entrez Gene ID	779457
Full Name	DNA topoisomerase II beta
General protein information	Preferred Names DNA topoisomerase II beta Names DNA topoisomerase 2-beta topoisomerase (DNA) II beta 180kDa
Gene Type	protein-coding
Organism	Xenopus tropicalis(tropical clawed frog)

mRNA and Protein(s)

mRNA	Protein	Name
XM_018095006.2	XP_017950495.2	DNA topoisomerase 2-beta isoform X2
XM_002932410.4	XP_002932456.2	DNA topoisomerase 2-beta isoform X1
XM_002932410.5	XP_002932456.3	DNA topoisomerase 2-beta isoform X1
XM_018095006.1	XP_017950495.1	DNA topoisomerase 2-beta isoform X2

Pathways from BioSystems

Homologies

Schizosaccharomyces pombe (fission yeast)	top2	NP_595805.1
Oryza sativa (rice)	Os02g0699700	NP_001047833.1
Saccharomyces cerevisiae (baker's yeast)	TOP2	NP_014311.3
Neurospora crassa	NCU06338	XP_962372.2
Pan troglodytes (chimpanzee)	TOP2B	XP_516332.3
Macaca mulatta (Rhesus monkey)	TOP2B	XP_001092092.2
Gallus gallus (chicken)	TOP2B	NP_990413.1
Danio rerio (zebrafish)	top2b	NP_001038656.1
Magnaporthe oryzae (rice blast fungus)	MGG_06162	XP_003712042.1
Bos taurus (cattle)	TOP2B	XP_002698796.2
Mus musculus (house mouse)	Top2b	NP_033435.2
Arabidopsis thaliana (thale cress)	TOPII	NP_001118686.1
Kluyveromyces lactis	KLLA0C13838g	XP_452818.1
Eremothecium gossypii	AGOS_AER410W	NP_985265.1
Homo sapiens (human)	TOP2B	NP_001059.2
Canis lupus familiaris (dog)	TOP2B	XP_534241.3
Rattus norvegicus (Norway rat)	Top2b	NP_001094328.1
Xenopus tropicalis (tropical clawed frog)	top2b	XP_004915443.1

Gene Ontology

The following top2b gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the top2b cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OXa11913	XM_002932410.4 Latest version!	Xenopus tropicalis topoisomerase (DNA) II beta (top2b), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OXa45162	XM_018095006.1 Latest version!	Xenopus tropicalis topoisomerase (DNA) II beta (top2b), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OXa60892	XM_002932410.5 Latest version!	Xenopus tropicalis DNA topoisomerase II beta (top2b), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OXa60893	XM_018095006.2 Latest version!	Xenopus tropicalis DNA topoisomerase II beta (top2b), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

top2b ( XM_002932410.4 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_002932456.2 Xenopus tropicalis topoisomerase (DNA) II beta (top2b), transcript variant X1, mRNA.

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CloneID	OXa11913
Clone ID Related Accession (Same CDS sequence)	XM_002932410.4
Accession Version	XM_002932410.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5097bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1473177600000
Organism	Xenopus tropicalis(tropical clawed frog)
Product	DNA topoisomerase 2-beta isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030682.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_002932410.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTAAAT CTGGCGGCGG CGGAGGAGCT GGAGGAGGGA GCGGAGGGCT CACCTGGGTG 
ACACTTTTTG ATAATCAGAA CGCATCAAAA AAGGAAGAAA CAGAAAGCAA TAATAAAAAT 
GACTCCTCCA AGAAGATGTC CGTAGAAAGA GTTTACCAGA AAAAGACCCA GCTTGAGCAT 
ATTCTTCTGC GACCTGACAC ATACATTGGA TCTGTGGAGA CAGTTACTCA GTCCATGTGG 
GTTTATGATG AAGATGTTGG AATGAATTGC CGTGACATAA CCTATGTGCC CGGTTTATAC 
AAGATATTTG ATGAAATCCT TGTAAACGCA GCTGATAATA AACAGAGAGA TAAAAATATG 
AACTGCATAA AAATTTCTAT TGATCCTGAT TCTAACATCA TCAGCATTTG GAATAATGGT 
AAAGGAATAC CTGTGGTTGA GCACAAAGTG GAGAAAATGT ATGTCCCTGC TTTAATTTTT 
GGCCAGTTAT TAACATCAAG CAACTATGAT GATGAGGAAA AGAAAGTTAC AGGAGGGCGA 
AATGGATATG GTGCTAAACT TTGCAATATA TTCAGCACAA AATTTACTGT GGAGACAGCT 
TGTAAGGAAT ACAAGCATAG CTTTAAGCAG ACATGGACAA ACAACATGTT AAAGACAACT 
GAACCCAAAA TTAAACATTT TGATGGAGAT GACTACACAT GCATAACATT CCAGCCAGAT 
TTAGCCAAGT TCCAAATGGA AAAACTGGAC AAGGATACTG TGGCTCTTCT GACAAGACGA 
GCATATGATA TTGCGGGTTC CTGCAAAGGA GTCAAAGTCT TGCTCAATGG GAAGAAGCTT 
CCGGTAAATG GCTTCCGCAG TTACGTAGAT CTTTACGTAA AGGACAGACT TGATGAAACT 
GGTGTCGCTC TTAAAGTAAT CCATGAAGTT GCAAATGAAA GATGGGATGT ATGTTTAACA 
TTGAGCGAAA AGGGATTCCA ACAAATCAGT TTTGTGAACA GTATTGCTAC CACAAAGGGT 
GGTAGACATG TAGATTATGT AGTAGACCAA ATTGTGTCAA AACTTATTGA AGTTGTGAAG 
AAGAAGAATA AAGCTGGAGT TTCTGTAAAG CCCTTCCAGG TGAAGAATCA CGTTTGGGTT 
TTTGTCAACT GTTTAATAGA AAACCCAACT TTTGATTCTC AGACAAAAGA AAACATGACA 
CTACAGCCTA AAGGTTTTGG GTCCAAATGC CAGCTGTCAG AAAAATTTTC AAAAGCGGCA 
TCTAACTGTG GCATAGTCGA GAGTATTCTA AATTGGGTTA AATTCAAGGC CCAAACACAG 
CTAAATAAGA AATGCTCTTC TGTGAAACAC AGTAAAATCA AAGGCATTCC CAAGCTGGAT 
GATGCTAATG ATGCAGGCGG AAAACATTCC CTGGACTGCA CTCTGATATT AACTGAGGGA 
GACTCTGCCA AATCTTTAGC TGTTTCAGGC TTAGGTGTCA TTGGCCGAGA TCGGTACGGT 
GTATTCCCGC TCAGGGGAAA AATTCTCAAT GTTCGAGAAG CTTCCCACAA ACAGATTATG 
GAAAATGCTG AGATAAACAA CATCATTAAA ATAGTTGGCT TGCAGTACAA AAAAAGCTAT 
GATGATAATG AATCCCTTAA AACCTTGCGT TATGGAAAAA TTATGATAAT GACAGATCAG 
GATCAAGATG GCTCGCATAT CAAGGGCTTA TTGATTAATT TTTTCCATCA CAATTGGCCA 
TCTCTACTGA AACATAGTTT CCTTGAAGAA TTTATCACAC CCATTGTTAA GGCTACAAAG 
AATAAGCAGG AGCTTTCATT CTACAGTATT CCTGAGTTTG AAGAATGGAA AAAACAAACA 
GAAAACCAAA AATTGTGGAA GATTAAGTAT TACAAAGGTT TGGGCACCAG CACTTCGAAG 
GAAGCAAAGG AATACTTTGC TGACATGGAC AGGCACAGAA TTATATTTAG ATATGCAGGC 
CCTGAAGATG ATGCAGCTAT CACTCTGGCT TTCAGCAAAA AGAAAGTAGA TGACAGGAAA 
GAATGGCTCA CAAACTTTAT GGAAGACAGG CGACAACGTC GACTACACGG ATTGCCCGAG 
CAATTCTTGT ACGGCACAGC TACAAAACAC TTGACTTACA ATGACTTCAT TAACAAGGAG 
CTGATTCTTT TTTCAAACTC TGATAATGAG CGATCTATTC CATCTCTTGT TGATGGTTTA 
AAGCCTGGGC AAAGAAAAGT TCTGTTTACT TGTTTTAAAA GGAATGATAA ACGGGAAGTC 
AAGGTTGCTC AGCTGGCTGG TTCTGTTGCT GAAATGTCCG CTTACCACCA TGGAGAGCAA 
GCCTTGATGA TGACAATAGT AAATCTTGCC CAGAACTTTG TGGGAAGTAA CAATGTGAGC 
TTGCTGCAGC CCATTGGTCA GTTTGGTACT CGACTCCATG GTGGAAAGGA TGCTGCCAGC 
CCTCGTTATA TATTCACAAT GTTAAGTCCT CTAGCAAGAT TGCTCTATCC ATCTGTGGAT 
GATAACCTTT TGAAGTTCCT TTATGATGAT AACCAGCGAG TAGAACCAGA GTGGTACATT 
CCTATTATAC CCCTGGTTTT AGTTAATGGA GCAGAAGGAA TCGGTACTGG ATGGGCTTGT 
AAACTGCCCA ATTATGACAC AAGAGAAATT GTCAATAATG TGAAGAGAAT GCTGGATGGT 
CTTGATCCTT ATCCATTGCT TCCAAGCTAC AAGAATTTTA AAGGTACGAT TCAGGAACTG 
GGACAGAATC AGTATGCAAT GAGTGGTGAA ATATATGTGC TGGACCGGAA CACAATTGAA 
ATCACAGAAC TTCCTGTCAG GACATGGACC CAGGTTTACA AAGAACAAGT GCTGGAGCCC 
ATGCTCAATG GAACCGAGAA AACACCATCG TTAATCTCTG ATTACAAAGA GTATCATACA 
GACACAACTG TAAAATTTGT TGTCAAAATG ACAGAAGAAA AGTTGGCACA GGCAGAAGCT 
GCTGGCTTGC ATAAAGTCTT TAAACTTCAA ACAAGTCTTA CTTGCAATTC TATGGTATTA 
TTTGACCACA TGGGCTGTCT AAAGAAATAT GAAACCGTGC AGGACATTCT GAAGGAATTT 
TTTGATCTCC GGTTAAATTA CTACACCCTG CGCAAAGAAT GGCTGATAGG AATGTTGGGA 
GCTGAAGCTG CAAGACTGAA CAACCAAGCC CGGTTTGTCC TGGAGAAGAT ACAAGGCAAA 
ATCTCTATAG AGAACAAAAC CAAAAGAGAT CTAATTCAGA TGTTGGTGCA GAAAGGATTT 
GAGTCAGACC CTGTGAAAGC TTGGAAGGAG GCACAAGAAA AAGCAACAGA GGAGGATGAC 
CTTCAAGATC CGAATGAAGA CAATTCATCC GACTCTGGCA CTGGATCAGG ACCGGATTTT 
AACTACATTC TCAATATGTC TCTCTGGAGC CTTTCAAAGG AAAAAGTAGA TGAGCTTGTC 
AGACAGAGGG ATATAAAGAG CAAAGAACTG AATGACCTCA AGAGGAAGTC TCCGCCTGAT 
CTGTGGAAGG AAGACTTGGC AGCTTTTGTT GAAGAGCTAG ACAGAGTGGA AGCTCAAGAA 
AAAGAAGATG TCTTAGCAGG GTTTGCAGGC AAAGCAATAA AAGGCAAAAT TGGAAAACCC 
AAAATGAAGA AATTACAGCT TGAGGAGACT TTGCCATCTC TTTACGGCAG AAGAGTAGAG 
CCACAAATCA GTGCAATGAA AGCAGATGCC AGCAAAAAGA TGCTAAAGAA AAAGAAGGGT 
GAAATTGACT CTATTGCACT GAAAATGGAA TTTGATGAGG AGTTTAGCTC GGCACCAGCA 
GAGGGAGGAG CTGAAGATTC CATGAATACC ACTCCTGTAA AACCTGTAAA GCCCAAGAGA 
GAAAGAAAAG AACCAGGTCC TCGAGCCAAA AAGACTGTAT TAACACCCAA GTCATCAACC 
AAGAAAGGGA AGAAGCGAAA CCCTTGGTCA GACAATGAGT CAAAATCAGA CAGTGACCTG 
GAAGAAGCTG AGGCAGAGCC TGTGGTCATT CCTCGGGACT CTCTCCTAAG GAGAGCAGCT 
GCTGAAAGAC CGAAGTACAC GTTTGATTTC TCTGAAGAGG AAGATGCAGA CGATGACGAC 
GAAGGTGTCA TTAGCCGTAG TAATAATAAT AATAATATTA ATGACTTAAA ACCGCCAGCT 
TCTCCTGCTG CTAATAGCCG AGGTGAAGAT GATGATGTTC TCTCTGACAG CCAAGAAAAG 
GATGACTATG AATTCTCCCC TTTCAAGTCT ACCCCAGCCG CACAAAAGCC TTCAGAAACA 
CAAAAGAAAA AAGACTACAT ATTTTCATCA TCTTCATTTT CAGAAAAAGT AGAAGATTCT 
GCAATATATA ACAGTGACGA TGATGAACCC TCCCCACTTT ACTCTTCGTA CTCCTTCAAA 
ACTTCAGAGA AAACAACAGG CAAAGGATCT AGTAAAAAAG AATCTGCAAA ATATGACAGT 
GATGATGATG ATGATGATGA TGAACCCTCC CCCCTTTACT CCTCATATTC CTTCAAATCT 
ACAGAAAAGC CAGCCAGCAA AGGATCTAGT GAGAAAGAAT CCACCAAATA TAACAGTGAC 
GATGAGGATG ATGACGATGA CGATGTACCG TTTTCGTCGT TTACCTTTAA ATCTTCCGAA 
AAGCCAGCCA GTAAAGGTTC TGCTAAGAAA ACAAAATCTT TGTCAGATGG TACTCCTAAA 
CCAAAACGAC CGCCAAAACC AAAGAAGTCT GAGACTTCAT TGATTTCCGA CTCTGACTTG 
TTAGATTTTG GTGCATCAAA GAAATCTGCA ACACCCAAAG CAAAAGGAAG GGGAAGGAAA 
CGAAAAACAT CTGATTCTGA AAATGAAGGA GATTACAATC CAGGGCGAAA ACCTACAAAA 
CCTGCATCTT CTAGCAAAAA ATCCAAGAAA GCATCATTTG AGCAGGATTC AGATGGTGAT 
CTCTTCCCTT CAGAAGTCGG CTCTGAGACC GCATCGAGGC AAAGGCCTGG CCGGGCTAGG 
AAAGAAGTCA AATATTTTGC AGACTCTGAC GATGAAGATG ATTTTGCTAT GTTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_002932456.2
CDS	835..5931
Translation	MSKSGGGGGAGGGSGGLTWVTLFDNQNASKKEETESNNKNDSSKKMSVERVYQKKTQLEHILLRPDTYIGSVETVTQSMWVYDEDVGMNCRDITYVPGLYKIFDEILVNAADNKQRDKNMNCIKISIDPDSNIISIWNNGKGIPVVEHKVEKMYVPALIFGQLLTSSNYDDEEKKVTGGRNGYGAKLCNIFSTKFTVETACKEYKHSFKQTWTNNMLKTTEPKIKHFDGDDYTCITFQPDLAKFQMEKLDKDTVALLTRRAYDIAGSCKGVKVLLNGKKLPVNGFRSYVDLYVKDRLDETGVALKVIHEVANERWDVCLTLSEKGFQQISFVNSIATTKGGRHVDYVVDQIVSKLIEVVKKKNKAGVSVKPFQVKNHVWVFVNCLIENPTFDSQTKENMTLQPKGFGSKCQLSEKFSKAASNCGIVESILNWVKFKAQTQLNKKCSSVKHSKIKGIPKLDDANDAGGKHSLDCTLILTEGDSAKSLAVSGLGVIGRDRYGVFPLRGKILNVREASHKQIMENAEINNIIKIVGLQYKKSYDDNESLKTLRYGKIMIMTDQDQDGSHIKGLLINFFHHNWPSLLKHSFLEEFITPIVKATKNKQELSFYSIPEFEEWKKQTENQKLWKIKYYKGLGTSTSKEAKEYFADMDRHRIIFRYAGPEDDAAITLAFSKKKVDDRKEWLTNFMEDRRQRRLHGLPEQFLYGTATKHLTYNDFINKELILFSNSDNERSIPSLVDGLKPGQRKVLFTCFKRNDKREVKVAQLAGSVAEMSAYHHGEQALMMTIVNLAQNFVGSNNVSLLQPIGQFGTRLHGGKDAASPRYIFTMLSPLARLLYPSVDDNLLKFLYDDNQRVEPEWYIPIIPLVLVNGAEGIGTGWACKLPNYDTREIVNNVKRMLDGLDPYPLLPSYKNFKGTIQELGQNQYAMSGEIYVLDRNTIEITELPVRTWTQVYKEQVLEPMLNGTEKTPSLISDYKEYHTDTTVKFVVKMTEEKLAQAEAAGLHKVFKLQTSLTCNSMVLFDHMGCLKKYETVQDILKEFFDLRLNYYTLRKEWLIGMLGAEAARLNNQARFVLEKIQGKISIENKTKRDLIQMLVQKGFESDPVKAWKEAQEKATEEDDLQDPNEDNSSDSGTGSGPDFNYILNMSLWSLSKEKVDELVRQRDIKSKELNDLKRKSPPDLWKEDLAAFVEELDRVEAQEKEDVLAGFAGKAIKGKIGKPKMKKLQLEETLPSLYGRRVEPQISAMKADASKKMLKKKKGEIDSIALKMEFDEEFSSAPAEGGAEDSMNTTPVKPVKPKRERKEPGPRAKKTVLTPKSSTKKGKKRNPWSDNESKSDSDLEEAEAEPVVIPRDSLLRRAAAERPKYTFDFSEEEDADDDDEGVISRSNNNNNINDLKPPASPAANSRGEDDDVLSDSQEKDDYEFSPFKSTPAAQKPSETQKKKDYIFSSSSFSEKVEDSAIYNSDDDEPSPLYSSYSFKTSEKTTGKGSSKKESAKYDSDDDDDDDEPSPLYSSYSFKSTEKPASKGSSEKESTKYNSDDEDDDDDDVPFSSFTFKSSEKPASKGSAKKTKSLSDGTPKPKRPPKPKKSETSLISDSDLLDFGASKKSATPKAKGRGRKRKTSDSENEGDYNPGRKPTKPASSSKKSKKASFEQDSDGDLFPSEVGSETASRQRPGRARKEVKYFADSDDEDDFAMF

Target ORF information:

RefSeq Version	XM_002932410.4
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis topoisomerase (DNA) II beta (top2b), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_002932410.4

ORF Insert Sequence:

ATGTCTAAAT CTGGCGGCGG CGGAGGAGCT GGAGGAGGGA GCGGAGGGCT CACCTGGGTG 
ACACTTTTTG ATAATCAGAA CGCATCAAAA AAGGAAGAAA CAGAAAGCAA TAATAAAAAT 
GACTCCTCCA AGAAGATGTC CGTAGAAAGA GTTTACCAGA AAAAGACCCA GCTTGAGCAT 
ATTCTTCTGC GACCTGACAC ATACATTGGA TCTGTGGAGA CAGTTACTCA GTCCATGTGG 
GTTTATGATG AAGATGTTGG AATGAATTGC CGTGACATAA CCTATGTGCC CGGTTTATAC 
AAGATATTTG ATGAAATCCT TGTAAACGCA GCTGATAATA AACAGAGAGA TAAAAATATG 
AACTGCATAA AAATTTCTAT TGATCCTGAT TCTAACATCA TCAGCATTTG GAATAATGGT 
AAAGGAATAC CTGTGGTTGA GCACAAAGTG GAGAAAATGT ATGTCCCTGC TTTAATTTTT 
GGCCAGTTAT TAACATCAAG CAACTATGAT GATGAGGAAA AGAAAGTTAC AGGAGGGCGA 
AATGGATATG GTGCTAAACT TTGCAATATA TTCAGCACAA AATTTACTGT GGAGACAGCT 
TGTAAGGAAT ACAAGCATAG CTTTAAGCAG ACATGGACAA ACAACATGTT AAAGACAACT 
GAACCCAAAA TTAAACATTT TGATGGAGAT GACTACACAT GCATAACATT CCAGCCAGAT 
TTAGCCAAGT TCCAAATGGA AAAACTGGAC AAGGATACTG TGGCTCTTCT GACAAGACGA 
GCATATGATA TTGCGGGTTC CTGCAAAGGA GTCAAAGTCT TGCTCAATGG GAAGAAGCTT 
CCGGTAAATG GCTTCCGCAG TTACGTAGAT CTTTACGTAA AGGACAGACT TGATGAAACT 
GGTGTCGCTC TTAAAGTAAT CCATGAAGTT GCAAATGAAA GATGGGATGT ATGTTTAACA 
TTGAGCGAAA AGGGATTCCA ACAAATCAGT TTTGTGAACA GTATTGCTAC CACAAAGGGT 
GGTAGACATG TAGATTATGT AGTAGACCAA ATTGTGTCAA AACTTATTGA AGTTGTGAAG 
AAGAAGAATA AAGCTGGAGT TTCTGTAAAG CCCTTCCAGG TGAAGAATCA CGTTTGGGTT 
TTTGTCAACT GTTTAATAGA AAACCCAACT TTTGATTCTC AGACAAAAGA AAACATGACA 
CTACAGCCTA AAGGTTTTGG GTCCAAATGC CAGCTGTCAG AAAAATTTTC AAAAGCGGCA 
TCTAACTGTG GCATAGTCGA GAGTATTCTA AATTGGGTTA AATTCAAGGC CCAAACACAG 
CTAAATAAGA AATGCTCTTC TGTGAAACAC AGTAAAATCA AAGGCATTCC CAAGCTGGAT 
GATGCTAATG ATGCAGGCGG AAAACATTCC CTGGACTGCA CTCTGATATT AACTGAGGGA 
GACTCTGCCA AATCTTTAGC TGTTTCAGGC TTAGGTGTCA TTGGCCGAGA TCGGTACGGT 
GTATTCCCGC TCAGGGGAAA AATTCTCAAT GTTCGAGAAG CTTCCCACAA ACAGATTATG 
GAAAATGCTG AGATAAACAA CATCATTAAA ATAGTTGGCT TGCAGTACAA AAAAAGCTAT 
GATGATAATG AATCCCTTAA AACCTTGCGT TATGGAAAAA TTATGATAAT GACAGATCAG 
GATCAAGATG GCTCGCATAT CAAGGGCTTA TTGATTAATT TTTTCCATCA CAATTGGCCA 
TCTCTACTGA AACATAGTTT CCTTGAAGAA TTTATCACAC CCATTGTTAA GGCTACAAAG 
AATAAGCAGG AGCTTTCATT CTACAGTATT CCTGAGTTTG AAGAATGGAA AAAACAAACA 
GAAAACCAAA AATTGTGGAA GATTAAGTAT TACAAAGGTT TGGGCACCAG CACTTCGAAG 
GAAGCAAAGG AATACTTTGC TGACATGGAC AGGCACAGAA TTATATTTAG ATATGCAGGC 
CCTGAAGATG ATGCAGCTAT CACTCTGGCT TTCAGCAAAA AGAAAGTAGA TGACAGGAAA 
GAATGGCTCA CAAACTTTAT GGAAGACAGG CGACAACGTC GACTACACGG ATTGCCCGAG 
CAATTCTTGT ACGGCACAGC TACAAAACAC TTGACTTACA ATGACTTCAT TAACAAGGAG 
CTGATTCTTT TTTCAAACTC TGATAATGAG CGATCTATTC CATCTCTTGT TGATGGTTTA 
AAGCCTGGGC AAAGAAAAGT TCTGTTTACT TGTTTTAAAA GGAATGATAA ACGGGAAGTC 
AAGGTTGCTC AGCTGGCTGG TTCTGTTGCT GAAATGTCCG CTTACCACCA TGGAGAGCAA 
GCCTTGATGA TGACAATAGT AAATCTTGCC CAGAACTTTG TGGGAAGTAA CAATGTGAGC 
TTGCTGCAGC CCATTGGTCA GTTTGGTACT CGACTCCATG GTGGAAAGGA TGCTGCCAGC 
CCTCGTTATA TATTCACAAT GTTAAGTCCT CTAGCAAGAT TGCTCTATCC ATCTGTGGAT 
GATAACCTTT TGAAGTTCCT TTATGATGAT AACCAGCGAG TAGAACCAGA GTGGTACATT 
CCTATTATAC CCCTGGTTTT AGTTAATGGA GCAGAAGGAA TCGGTACTGG ATGGGCTTGT 
AAACTGCCCA ATTATGACAC AAGAGAAATT GTCAATAATG TGAAGAGAAT GCTGGATGGT 
CTTGATCCTT ATCCATTGCT TCCAAGCTAC AAGAATTTTA AAGGTACGAT TCAGGAACTG 
GGACAGAATC AGTATGCAAT GAGTGGTGAA ATATATGTGC TGGACCGGAA CACAATTGAA 
ATCACAGAAC TTCCTGTCAG GACATGGACC CAGGTTTACA AAGAACAAGT GCTGGAGCCC 
ATGCTCAATG GAACCGAGAA AACACCATCG TTAATCTCTG ATTACAAAGA GTATCATACA 
GACACAACTG TAAAATTTGT TGTCAAAATG ACAGAAGAAA AGTTGGCACA GGCAGAAGCT 
GCTGGCTTGC ATAAAGTCTT TAAACTTCAA ACAAGTCTTA CTTGCAATTC TATGGTATTA 
TTTGACCACA TGGGCTGTCT AAAGAAATAT GAAACCGTGC AGGACATTCT GAAGGAATTT 
TTTGATCTCC GGTTAAATTA CTACACCCTG CGCAAAGAAT GGCTGATAGG AATGTTGGGA 
GCTGAAGCTG CAAGACTGAA CAACCAAGCC CGGTTTGTCC TGGAGAAGAT ACAAGGCAAA 
ATCTCTATAG AGAACAAAAC CAAAAGAGAT CTAATTCAGA TGTTGGTGCA GAAAGGATTT 
GAGTCAGACC CTGTGAAAGC TTGGAAGGAG GCACAAGAAA AAGCAACAGA GGAGGATGAC 
CTTCAAGATC CGAATGAAGA CAATTCATCC GACTCTGGCA CTGGATCAGG ACCGGATTTT 
AACTACATTC TCAATATGTC TCTCTGGAGC CTTTCAAAGG AAAAAGTAGA TGAGCTTGTC 
AGACAGAGGG ATATAAAGAG CAAAGAACTG AATGACCTCA AGAGGAAGTC TCCGCCTGAT 
CTGTGGAAGG AAGACTTGGC AGCTTTTGTT GAAGAGCTAG ACAGAGTGGA AGCTCAAGAA 
AAAGAAGATG TCTTAGCAGG GTTTGCAGGC AAAGCAATAA AAGGCAAAAT TGGAAAACCC 
AAAATGAAGA AATTACAGCT TGAGGAGACT TTGCCATCTC TTTACGGCAG AAGAGTAGAG 
CCACAAATCA GTGCAATGAA AGCAGATGCC AGCAAAAAGA TGCTAAAGAA AAAGAAGGGT 
GAAATTGACT CTATTGCACT GAAAATGGAA TTTGATGAGG AGTTTAGCTC GGCACCAGCA 
GAGGGAGGAG CTGAAGATTC CATGAATACC ACTCCTGTAA AACCTGTAAA GCCCAAGAGA 
GAAAGAAAAG AACCAGGTCC TCGAGCCAAA AAGACTGTAT TAACACCCAA GTCATCAACC 
AAGAAAGGGA AGAAGCGAAA CCCTTGGTCA GACAATGAGT CAAAATCAGA CAGTGACCTG 
GAAGAAGCTG AGGCAGAGCC TGTGGTCATT CCTCGGGACT CTCTCCTAAG GAGAGCAGCT 
GCTGAAAGAC CGAAGTACAC GTTTGATTTC TCTGAAGAGG AAGATGCAGA CGATGACGAC 
GAAGGTGTCA TTAGCCGTAG TAATAATAAT AATAATATTA ATGACTTAAA ACCGCCAGCT 
TCTCCTGCTG CTAATAGCCG AGGTGAAGAT GATGATGTTC TCTCTGACAG CCAAGAAAAG 
GATGACTATG AATTCTCCCC TTTCAAGTCT ACCCCAGCCG CACAAAAGCC TTCAGAAACA 
CAAAAGAAAA AAGACTACAT ATTTTCATCA TCTTCATTTT CAGAAAAAGT AGAAGATTCT 
GCAATATATA ACAGTGACGA TGATGAACCC TCCCCACTTT ACTCTTCGTA CTCCTTCAAA 
ACTTCAGAGA AAACAACAGG CAAAGGATCT AGTAAAAAAG AATCTGCAAA ATATGACAGT 
GATGATGATG ATGATGATGA TGAACCCTCC CCCCTTTACT CCTCATATTC CTTCAAATCT 
ACAGAAAAGC CAGCCAGCAA AGGATCTAGT GAGAAAGAAT CCACCAAATA TAACAGTGAC 
GATGAGGATG ATGACGATGA CGATGTACCG TTTTCGTCGT TTACCTTTAA ATCTTCCGAA 
AAGCCAGCCA GTAAAGGTTC TGCTAAGAAA ACAAAATCTT TGTCAGATGG TACTCCTAAA 
CCAAAACGAC CGCCAAAACC AAAGAAGTCT GAGACTTCAT TGATTTCCGA CTCTGACTTG 
TTAGATTTTG GTGCATCAAA GAAATCTGCA ACACCCAAAG CAAAAGGAAG GGGAAGGAAA 
CGAAAAACAT CTGATTCTGA AAATGAAGGA GATTACAATC CAGGGCGAAA ACCTACAAAA 
CCTGCATCTT CTAGCAAAAA ATCCAAGAAA GCATCATTTG AGCAGGATTC AGATGGTGAT 
CTCTTCCCTT CAGAAGTCGG CTCTGAGACC GCATCGAGGC AAAGGCCTGG CCGGGCTAGG 
AAAGAAGTCA AATATTTTGC AGACTCTGAC GATGAAGATG ATTTTGCTAT GTTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

top2b ( XM_018095006.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_017950495.1 Xenopus tropicalis topoisomerase (DNA) II beta (top2b), transcript variant X2, mRNA.

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CloneID	OXa45162
Clone ID Related Accession (Same CDS sequence)	XM_018095006.1
Accession Version	XM_018095006.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4992bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1473177600000
Organism	Xenopus tropicalis(tropical clawed frog)
Product	DNA topoisomerase 2-beta isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030682.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTAAAT CTGGCGGCGG CGGAGGAGCT GGAGGAGGGA GCGGAGGGCT CACCTGGGTG 
ACACTTTTTG ATAATCAGAA CGCATCAAAA AAGGAAGAAA CAGAAAGCAA TAATAAAAAT 
GACTCCTCCA AGAAGATGTC CGTAGAAAGA GTTTACCAGA AAAAGACCCA GCTTGAGCAT 
ATTCTTCTGC GACCTGACAC ATACATTGGA TCTGTGGAGA CAGTTACTCA GTCCATGTGG 
GTTTATGATG AAGATGTTGG AATGAATTGC CGTGACATAA CCTATGTGCC CGGTTTATAC 
AAGATATTTG ATGAAATCCT TGTAAACGCA GCTGATAATA AACAGAGAGA TAAAAATATG 
AACTGCATAA AAATTTCTAT TGATCCTGAT TCTAACATCA TCAGCATTTG GAATAATGGT 
AAAGGAATAC CTGTGGTTGA GCACAAAGTG GAGAAAATGT ATGTCCCTGC TTTAATTTTT 
GGCCAGTTAT TAACATCAAG CAACTATGAT GATGAGGAAA AGAAAGTTAC AGGAGGGCGA 
AATGGATATG GTGCTAAACT TTGCAATATA TTCAGCACAA AATTTACTGT GGAGACAGCT 
TGTAAGGAAT ACAAGCATAG CTTTAAGCAG ACATGGACAA ACAACATGTT AAAGACAACT 
GAACCCAAAA TTAAACATTT TGATGGAGAT GACTACACAT GCATAACATT CCAGCCAGAT 
TTAGCCAAGT TCCAAATGGA AAAACTGGAC AAGGATACTG TGGCTCTTCT GACAAGACGA 
GCATATGATA TTGCGGGTTC CTGCAAAGGA GTCAAAGTCT TGCTCAATGG GAAGAAGCTT 
CCGGTAAATG GCTTCCGCAG TTACGTAGAT CTTTACGTAA AGGACAGACT TGATGAAACT 
GGTGTCGCTC TTAAAGTAAT CCATGAAGTT GCAAATGAAA GATGGGATGT ATGTTTAACA 
TTGAGCGAAA AGGGATTCCA ACAAATCAGT TTTGTGAACA GTATTGCTAC CACAAAGGGT 
GGTAGACATG TAGATTATGT AGTAGACCAA ATTGTGTCAA AACTTATTGA AGTTGTGAAG 
AAGAAGAATA AAGCTGGAGT TTCTGTAAAG CCCTTCCAGG TGAAGAATCA CGTTTGGGTT 
TTTGTCAACT GTTTAATAGA AAACCCAACT TTTGATTCTC AGACAAAAGA AAACATGACA 
CTACAGCCTA AAGGTTTTGG GTCCAAATGC CAGCTGTCAG AAAAATTTTC AAAAGCGGCA 
TCTAACTGTG GCATAGTCGA GAGTATTCTA AATTGGGTTA AATTCAAGGC CCAAACACAG 
CTAAATAAGA AATGCTCTTC TGTGAAACAC AGTAAAATCA AAGGCATTCC CAAGCTGGAT 
GATGCTAATG ATGCAGGCGG AAAACATTCC CTGGACTGCA CTCTGATATT AACTGAGGGA 
GACTCTGCCA AATCTTTAGC TGTTTCAGGC TTAGGTGTCA TTGGCCGAGA TCGGTACGGT 
GTATTCCCGC TCAGGGGAAA AATTCTCAAT GTTCGAGAAG CTTCCCACAA ACAGATTATG 
GAAAATGCTG AGATAAACAA CATCATTAAA ATAGTTGGCT TGCAGTACAA AAAAAGCTAT 
GATGATAATG AATCCCTTAA AACCTTGCGT TATGGAAAAA TTATGATAAT GACAGATCAG 
GATCAAGATG GCTCGCATAT CAAGGGCTTA TTGATTAATT TTTTCCATCA CAATTGGCCA 
TCTCTACTGA AACATAGTTT CCTTGAAGAA TTTATCACAC CCATTGTTAA GGCTACAAAG 
AATAAGCAGG AGCTTTCATT CTACAGTATT CCTGAGTTTG AAGAATGGAA AAAACAAACA 
GAAAACCAAA AATTGTGGAA GATTAAGTAT TACAAAGGTT TGGGCACCAG CACTTCGAAG 
GAAGCAAAGG AATACTTTGC TGACATGGAC AGGCACAGAA TTATATTTAG ATATGCAGGC 
CCTGAAGATG ATGCAGCTAT CACTCTGGCT TTCAGCAAAA AGAAAGTAGA TGACAGGAAA 
GAATGGCTCA CAAACTTTAT GGAAGACAGG CGACAACGTC GACTACACGG ATTGCCCGAG 
CAATTCTTGT ACGGCACAGC TACAAAACAC TTGACTTACA ATGACTTCAT TAACAAGGAG 
CTGATTCTTT TTTCAAACTC TGATAATGAG CGATCTATTC CATCTCTTGT TGATGGTTTA 
AAGCCTGGGC AAAGAAAAGT TCTGTTTACT TGTTTTAAAA GGAATGATAA ACGGGAAGTC 
AAGGTTGCTC AGCTGGCTGG TTCTGTTGCT GAAATGTCCG CTTACCACCA TGGAGAGCAA 
GCCTTGATGA TGACAATAGT AAATCTTGCC CAGAACTTTG TGGGAAGTAA CAATGTGAGC 
TTGCTGCAGC CCATTGGTCA GTTTGGTACT CGACTCCATG GTGGAAAGGA TGCTGCCAGC 
CCTCGTTATA TATTCACAAT GTTAAGTCCT CTAGCAAGAT TGCTCTATCC ATCTGTGGAT 
GATAACCTTT TGAAGTTCCT TTATGATGAT AACCAGCGAG TAGAACCAGA GTGGTACATT 
CCTATTATAC CCCTGGTTTT AGTTAATGGA GCAGAAGGAA TCGGTACTGG ATGGGCTTGT 
AAACTGCCCA ATTATGACAC AAGAGAAATT GTCAATAATG TGAAGAGAAT GCTGGATGGT 
CTTGATCCTT ATCCATTGCT TCCAAGCTAC AAGAATTTTA AAGGTACGAT TCAGGAACTG 
GGACAGAATC AGTATGCAAT GAGTGGTGAA ATATATGTGC TGGACCGGAA CACAATTGAA 
ATCACAGAAC TTCCTGTCAG GACATGGACC CAGGTTTACA AAGAACAAGT GCTGGAGCCC 
ATGCTCAATG GAACCGAGAA AACACCATCG TTAATCTCTG ATTACAAAGA GTATCATACA 
GACACAACTG TAAAATTTGT TGTCAAAATG ACAGAAGAAA AGTTGGCACA GGCAGAAGCT 
GCTGGCTTGC ATAAAGTCTT TAAACTTCAA ACAAGTCTTA CTTGCAATTC TATGGTATTA 
TTTGACCACA TGGGCTGTCT AAAGAAATAT GAAACCGTGC AGGACATTCT GAAGGAATTT 
TTTGATCTCC GGTTAAATTA CTACACCCTG CGCAAAGAAT GGCTGATAGG AATGTTGGGA 
GCTGAAGCTG CAAGACTGAA CAACCAAGCC CGGTTTGTCC TGGAGAAGAT ACAAGGCAAA 
ATCTCTATAG AGAACAAAAC CAAAAGAGAT CTAATTCAGA TGTTGGTGCA GAAAGGATTT 
GAGTCAGACC CTGTGAAAGC TTGGAAGGAG GCACAAGAAA AAGCAACAGA GGAGGATGAC 
CTTCAAGATC CGAATGAAGA CAATTCATCC GACTCTGGCA CTGGATCAGG ACCGGATTTT 
AACTACATTC TCAATATGTC TCTCTGGAGC CTTTCAAAGG AAAAAGTAGA TGAGCTTGTC 
AGACAGAGGG ATATAAAGAG CAAAGAACTG AATGACCTCA AGAGGAAGTC TCCGCCTGAT 
CTGTGGAAGG AAGACTTGGC AGCTTTTGTT GAAGAGCTAG ACAGAGTGGA AGCTCAAGAA 
AAAGAAGATG TCTTAGCAGG GTTTGCAGGC AAAGCAATAA AAGGCAAAAT TGGAAAACCC 
AAAATGAAGA AATTACAGCT TGAGGAGACT TTGCCATCTC TTTACGGCAG AAGAGTAGAG 
CCACAAATCA GTGCAATGAA AGCAGATGCC AGCAAAAAGA TGCTAAAGAA AAAGAAGGGT 
GAAATTGACT CTATTGCACT GAAAATGGAA TTTGATGAGG AGTTTAGCTC GGCACCAGCA 
GAGGGAGGAG CTGAAGATTC CATGAATACC ACTCCTGTAA AACCTGTAAA GCCCAAGAGA 
GAAAGAAAAG AACCAGGTCC TCGAGCCAAA AAGACTGTAT TAACACCCAA GTCATCAACC 
AAGAAAGGGA AGAAGCGAAA CCCTTGGTCA GACAATGAGT CAAAATCAGA CAGTGACCTG 
GAAGAAGCTG AGGCAGAGCC TGTGGTCATT CCTCGGGACT CTCTCCTAAG GAGAGCAGCT 
GCTGAAAGAC CGAAGTACAC GTTTGATTTC TCTGAAGAGG AAGATGCAGA CGATGACGAC 
GAAGGTGTCA TTAGCCGTAG TAATAATAAT AATAATATTA ATGACTTAAA ACCGCCAGCT 
TCTCCTGCTG CTAATAGCCG AGGTGAAGAT GATGATGTTC TCTCTGACAG CCAAGAAAAG 
GATGACTATG AATTCTCCCC TTTCAAGTCT ACCCCAGCCG CACAAAAGCC TTCAGAAACA 
CAAAAGAAAA AAGACTACAT ATTTTCATCA TCTTCATTTT CAGAAAAAGT AGAAGAATCT 
GCAAAATATG ACAGTGATGA TGATGATGAT GATGATGAAC CCTCCCCCCT TTACTCCTCA 
TATTCCTTCA AATCTACAGA AAAGCCAGCC AGCAAAGGAT CTAGTGAGAA AGAATCCACC 
AAATATAACA GTGACGATGA GGATGATGAC GATGACGATG TACCGTTTTC GTCGTTTACC 
TTTAAATCTT CCGAAAAGCC AGCCAGTAAA GGTTCTGCTA AGAAAACAAA ATCTTTGTCA 
GATGGTACTC CTAAACCAAA ACGACCGCCA AAACCAAAGA AGTCTGAGAC TTCATTGATT 
TCCGACTCTG ACTTGTTAGA TTTTGGTGCA TCAAAGAAAT CTGCAACACC CAAAGCAAAA 
GGAAGGGGAA GGAAACGAAA AACATCTGAT TCTGAAAATG AAGGAGATTA CAATCCAGGG 
CGAAAACCTA CAAAACCTGC ATCTTCTAGC AAAAAATCCA AGAAAGCATC ATTTGAGCAG 
GATTCAGATG GTGATCTCTT CCCTTCAGAA GTCGGCTCTG AGACCGCATC GAGGCAAAGG 
CCTGGCCGGG CTAGGAAAGA AGTCAAATAT TTTGCAGACT CTGACGATGA AGATGATTTT 
GCTATGTTTT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017950495.1
CDS	840..5831
Translation	MSKSGGGGGAGGGSGGLTWVTLFDNQNASKKEETESNNKNDSSKKMSVERVYQKKTQLEHILLRPDTYIGSVETVTQSMWVYDEDVGMNCRDITYVPGLYKIFDEILVNAADNKQRDKNMNCIKISIDPDSNIISIWNNGKGIPVVEHKVEKMYVPALIFGQLLTSSNYDDEEKKVTGGRNGYGAKLCNIFSTKFTVETACKEYKHSFKQTWTNNMLKTTEPKIKHFDGDDYTCITFQPDLAKFQMEKLDKDTVALLTRRAYDIAGSCKGVKVLLNGKKLPVNGFRSYVDLYVKDRLDETGVALKVIHEVANERWDVCLTLSEKGFQQISFVNSIATTKGGRHVDYVVDQIVSKLIEVVKKKNKAGVSVKPFQVKNHVWVFVNCLIENPTFDSQTKENMTLQPKGFGSKCQLSEKFSKAASNCGIVESILNWVKFKAQTQLNKKCSSVKHSKIKGIPKLDDANDAGGKHSLDCTLILTEGDSAKSLAVSGLGVIGRDRYGVFPLRGKILNVREASHKQIMENAEINNIIKIVGLQYKKSYDDNESLKTLRYGKIMIMTDQDQDGSHIKGLLINFFHHNWPSLLKHSFLEEFITPIVKATKNKQELSFYSIPEFEEWKKQTENQKLWKIKYYKGLGTSTSKEAKEYFADMDRHRIIFRYAGPEDDAAITLAFSKKKVDDRKEWLTNFMEDRRQRRLHGLPEQFLYGTATKHLTYNDFINKELILFSNSDNERSIPSLVDGLKPGQRKVLFTCFKRNDKREVKVAQLAGSVAEMSAYHHGEQALMMTIVNLAQNFVGSNNVSLLQPIGQFGTRLHGGKDAASPRYIFTMLSPLARLLYPSVDDNLLKFLYDDNQRVEPEWYIPIIPLVLVNGAEGIGTGWACKLPNYDTREIVNNVKRMLDGLDPYPLLPSYKNFKGTIQELGQNQYAMSGEIYVLDRNTIEITELPVRTWTQVYKEQVLEPMLNGTEKTPSLISDYKEYHTDTTVKFVVKMTEEKLAQAEAAGLHKVFKLQTSLTCNSMVLFDHMGCLKKYETVQDILKEFFDLRLNYYTLRKEWLIGMLGAEAARLNNQARFVLEKIQGKISIENKTKRDLIQMLVQKGFESDPVKAWKEAQEKATEEDDLQDPNEDNSSDSGTGSGPDFNYILNMSLWSLSKEKVDELVRQRDIKSKELNDLKRKSPPDLWKEDLAAFVEELDRVEAQEKEDVLAGFAGKAIKGKIGKPKMKKLQLEETLPSLYGRRVEPQISAMKADASKKMLKKKKGEIDSIALKMEFDEEFSSAPAEGGAEDSMNTTPVKPVKPKRERKEPGPRAKKTVLTPKSSTKKGKKRNPWSDNESKSDSDLEEAEAEPVVIPRDSLLRRAAAERPKYTFDFSEEEDADDDDEGVISRSNNNNNINDLKPPASPAANSRGEDDDVLSDSQEKDDYEFSPFKSTPAAQKPSETQKKKDYIFSSSSFSEKVEESAKYDSDDDDDDDEPSPLYSSYSFKSTEKPASKGSSEKESTKYNSDDEDDDDDDVPFSSFTFKSSEKPASKGSAKKTKSLSDGTPKPKRPPKPKKSETSLISDSDLLDFGASKKSATPKAKGRGRKRKTSDSENEGDYNPGRKPTKPASSSKKSKKASFEQDSDGDLFPSEVGSETASRQRPGRARKEVKYFADSDDEDDFAMF

Target ORF information:

RefSeq Version	XM_018095006.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis topoisomerase (DNA) II beta (top2b), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_018095006.1

ORF Insert Sequence:

ATGTCTAAAT CTGGCGGCGG CGGAGGAGCT GGAGGAGGGA GCGGAGGGCT CACCTGGGTG 
ACACTTTTTG ATAATCAGAA CGCATCAAAA AAGGAAGAAA CAGAAAGCAA TAATAAAAAT 
GACTCCTCCA AGAAGATGTC CGTAGAAAGA GTTTACCAGA AAAAGACCCA GCTTGAGCAT 
ATTCTTCTGC GACCTGACAC ATACATTGGA TCTGTGGAGA CAGTTACTCA GTCCATGTGG 
GTTTATGATG AAGATGTTGG AATGAATTGC CGTGACATAA CCTATGTGCC CGGTTTATAC 
AAGATATTTG ATGAAATCCT TGTAAACGCA GCTGATAATA AACAGAGAGA TAAAAATATG 
AACTGCATAA AAATTTCTAT TGATCCTGAT TCTAACATCA TCAGCATTTG GAATAATGGT 
AAAGGAATAC CTGTGGTTGA GCACAAAGTG GAGAAAATGT ATGTCCCTGC TTTAATTTTT 
GGCCAGTTAT TAACATCAAG CAACTATGAT GATGAGGAAA AGAAAGTTAC AGGAGGGCGA 
AATGGATATG GTGCTAAACT TTGCAATATA TTCAGCACAA AATTTACTGT GGAGACAGCT 
TGTAAGGAAT ACAAGCATAG CTTTAAGCAG ACATGGACAA ACAACATGTT AAAGACAACT 
GAACCCAAAA TTAAACATTT TGATGGAGAT GACTACACAT GCATAACATT CCAGCCAGAT 
TTAGCCAAGT TCCAAATGGA AAAACTGGAC AAGGATACTG TGGCTCTTCT GACAAGACGA 
GCATATGATA TTGCGGGTTC CTGCAAAGGA GTCAAAGTCT TGCTCAATGG GAAGAAGCTT 
CCGGTAAATG GCTTCCGCAG TTACGTAGAT CTTTACGTAA AGGACAGACT TGATGAAACT 
GGTGTCGCTC TTAAAGTAAT CCATGAAGTT GCAAATGAAA GATGGGATGT ATGTTTAACA 
TTGAGCGAAA AGGGATTCCA ACAAATCAGT TTTGTGAACA GTATTGCTAC CACAAAGGGT 
GGTAGACATG TAGATTATGT AGTAGACCAA ATTGTGTCAA AACTTATTGA AGTTGTGAAG 
AAGAAGAATA AAGCTGGAGT TTCTGTAAAG CCCTTCCAGG TGAAGAATCA CGTTTGGGTT 
TTTGTCAACT GTTTAATAGA AAACCCAACT TTTGATTCTC AGACAAAAGA AAACATGACA 
CTACAGCCTA AAGGTTTTGG GTCCAAATGC CAGCTGTCAG AAAAATTTTC AAAAGCGGCA 
TCTAACTGTG GCATAGTCGA GAGTATTCTA AATTGGGTTA AATTCAAGGC CCAAACACAG 
CTAAATAAGA AATGCTCTTC TGTGAAACAC AGTAAAATCA AAGGCATTCC CAAGCTGGAT 
GATGCTAATG ATGCAGGCGG AAAACATTCC CTGGACTGCA CTCTGATATT AACTGAGGGA 
GACTCTGCCA AATCTTTAGC TGTTTCAGGC TTAGGTGTCA TTGGCCGAGA TCGGTACGGT 
GTATTCCCGC TCAGGGGAAA AATTCTCAAT GTTCGAGAAG CTTCCCACAA ACAGATTATG 
GAAAATGCTG AGATAAACAA CATCATTAAA ATAGTTGGCT TGCAGTACAA AAAAAGCTAT 
GATGATAATG AATCCCTTAA AACCTTGCGT TATGGAAAAA TTATGATAAT GACAGATCAG 
GATCAAGATG GCTCGCATAT CAAGGGCTTA TTGATTAATT TTTTCCATCA CAATTGGCCA 
TCTCTACTGA AACATAGTTT CCTTGAAGAA TTTATCACAC CCATTGTTAA GGCTACAAAG 
AATAAGCAGG AGCTTTCATT CTACAGTATT CCTGAGTTTG AAGAATGGAA AAAACAAACA 
GAAAACCAAA AATTGTGGAA GATTAAGTAT TACAAAGGTT TGGGCACCAG CACTTCGAAG 
GAAGCAAAGG AATACTTTGC TGACATGGAC AGGCACAGAA TTATATTTAG ATATGCAGGC 
CCTGAAGATG ATGCAGCTAT CACTCTGGCT TTCAGCAAAA AGAAAGTAGA TGACAGGAAA 
GAATGGCTCA CAAACTTTAT GGAAGACAGG CGACAACGTC GACTACACGG ATTGCCCGAG 
CAATTCTTGT ACGGCACAGC TACAAAACAC TTGACTTACA ATGACTTCAT TAACAAGGAG 
CTGATTCTTT TTTCAAACTC TGATAATGAG CGATCTATTC CATCTCTTGT TGATGGTTTA 
AAGCCTGGGC AAAGAAAAGT TCTGTTTACT TGTTTTAAAA GGAATGATAA ACGGGAAGTC 
AAGGTTGCTC AGCTGGCTGG TTCTGTTGCT GAAATGTCCG CTTACCACCA TGGAGAGCAA 
GCCTTGATGA TGACAATAGT AAATCTTGCC CAGAACTTTG TGGGAAGTAA CAATGTGAGC 
TTGCTGCAGC CCATTGGTCA GTTTGGTACT CGACTCCATG GTGGAAAGGA TGCTGCCAGC 
CCTCGTTATA TATTCACAAT GTTAAGTCCT CTAGCAAGAT TGCTCTATCC ATCTGTGGAT 
GATAACCTTT TGAAGTTCCT TTATGATGAT AACCAGCGAG TAGAACCAGA GTGGTACATT 
CCTATTATAC CCCTGGTTTT AGTTAATGGA GCAGAAGGAA TCGGTACTGG ATGGGCTTGT 
AAACTGCCCA ATTATGACAC AAGAGAAATT GTCAATAATG TGAAGAGAAT GCTGGATGGT 
CTTGATCCTT ATCCATTGCT TCCAAGCTAC AAGAATTTTA AAGGTACGAT TCAGGAACTG 
GGACAGAATC AGTATGCAAT GAGTGGTGAA ATATATGTGC TGGACCGGAA CACAATTGAA 
ATCACAGAAC TTCCTGTCAG GACATGGACC CAGGTTTACA AAGAACAAGT GCTGGAGCCC 
ATGCTCAATG GAACCGAGAA AACACCATCG TTAATCTCTG ATTACAAAGA GTATCATACA 
GACACAACTG TAAAATTTGT TGTCAAAATG ACAGAAGAAA AGTTGGCACA GGCAGAAGCT 
GCTGGCTTGC ATAAAGTCTT TAAACTTCAA ACAAGTCTTA CTTGCAATTC TATGGTATTA 
TTTGACCACA TGGGCTGTCT AAAGAAATAT GAAACCGTGC AGGACATTCT GAAGGAATTT 
TTTGATCTCC GGTTAAATTA CTACACCCTG CGCAAAGAAT GGCTGATAGG AATGTTGGGA 
GCTGAAGCTG CAAGACTGAA CAACCAAGCC CGGTTTGTCC TGGAGAAGAT ACAAGGCAAA 
ATCTCTATAG AGAACAAAAC CAAAAGAGAT CTAATTCAGA TGTTGGTGCA GAAAGGATTT 
GAGTCAGACC CTGTGAAAGC TTGGAAGGAG GCACAAGAAA AAGCAACAGA GGAGGATGAC 
CTTCAAGATC CGAATGAAGA CAATTCATCC GACTCTGGCA CTGGATCAGG ACCGGATTTT 
AACTACATTC TCAATATGTC TCTCTGGAGC CTTTCAAAGG AAAAAGTAGA TGAGCTTGTC 
AGACAGAGGG ATATAAAGAG CAAAGAACTG AATGACCTCA AGAGGAAGTC TCCGCCTGAT 
CTGTGGAAGG AAGACTTGGC AGCTTTTGTT GAAGAGCTAG ACAGAGTGGA AGCTCAAGAA 
AAAGAAGATG TCTTAGCAGG GTTTGCAGGC AAAGCAATAA AAGGCAAAAT TGGAAAACCC 
AAAATGAAGA AATTACAGCT TGAGGAGACT TTGCCATCTC TTTACGGCAG AAGAGTAGAG 
CCACAAATCA GTGCAATGAA AGCAGATGCC AGCAAAAAGA TGCTAAAGAA AAAGAAGGGT 
GAAATTGACT CTATTGCACT GAAAATGGAA TTTGATGAGG AGTTTAGCTC GGCACCAGCA 
GAGGGAGGAG CTGAAGATTC CATGAATACC ACTCCTGTAA AACCTGTAAA GCCCAAGAGA 
GAAAGAAAAG AACCAGGTCC TCGAGCCAAA AAGACTGTAT TAACACCCAA GTCATCAACC 
AAGAAAGGGA AGAAGCGAAA CCCTTGGTCA GACAATGAGT CAAAATCAGA CAGTGACCTG 
GAAGAAGCTG AGGCAGAGCC TGTGGTCATT CCTCGGGACT CTCTCCTAAG GAGAGCAGCT 
GCTGAAAGAC CGAAGTACAC GTTTGATTTC TCTGAAGAGG AAGATGCAGA CGATGACGAC 
GAAGGTGTCA TTAGCCGTAG TAATAATAAT AATAATATTA ATGACTTAAA ACCGCCAGCT 
TCTCCTGCTG CTAATAGCCG AGGTGAAGAT GATGATGTTC TCTCTGACAG CCAAGAAAAG 
GATGACTATG AATTCTCCCC TTTCAAGTCT ACCCCAGCCG CACAAAAGCC TTCAGAAACA 
CAAAAGAAAA AAGACTACAT ATTTTCATCA TCTTCATTTT CAGAAAAAGT AGAAGAATCT 
GCAAAATATG ACAGTGATGA TGATGATGAT GATGATGAAC CCTCCCCCCT TTACTCCTCA 
TATTCCTTCA AATCTACAGA AAAGCCAGCC AGCAAAGGAT CTAGTGAGAA AGAATCCACC 
AAATATAACA GTGACGATGA GGATGATGAC GATGACGATG TACCGTTTTC GTCGTTTACC 
TTTAAATCTT CCGAAAAGCC AGCCAGTAAA GGTTCTGCTA AGAAAACAAA ATCTTTGTCA 
GATGGTACTC CTAAACCAAA ACGACCGCCA AAACCAAAGA AGTCTGAGAC TTCATTGATT 
TCCGACTCTG ACTTGTTAGA TTTTGGTGCA TCAAAGAAAT CTGCAACACC CAAAGCAAAA 
GGAAGGGGAA GGAAACGAAA AACATCTGAT TCTGAAAATG AAGGAGATTA CAATCCAGGG 
CGAAAACCTA CAAAACCTGC ATCTTCTAGC AAAAAATCCA AGAAAGCATC ATTTGAGCAG 
GATTCAGATG GTGATCTCTT CCCTTCAGAA GTCGGCTCTG AGACCGCATC GAGGCAAAGG 
CCTGGCCGGG CTAGGAAAGA AGTCAAATAT TTTGCAGACT CTGACGATGA AGATGATTTT 
GCTATGTTTT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

top2b ( XM_002932410.5 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_002932456.3 Xenopus tropicalis DNA topoisomerase II beta (top2b), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OXa60892
Clone ID Related Accession (Same CDS sequence)	XM_002932410.5
Accession Version	XM_002932410.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5100bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1576512000000
Organism	Xenopus tropicalis(tropical clawed frog)
Product	DNA topoisomerase 2-beta isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030682.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_002932410.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTAAAT CTGGCGGCGG CGGCGGCGGA GGAGCTGGAG GAGGGAGCGG AGGGCTCACC 
TGGGTGACAC TTTTTGATAA TCAGAACGCA TCAAAAAAGG AAGAAACAGA AAGCAATAAT 
AAAAATGACT CCTCCAAGAA GATGTCCGTA GAAAGAGTTT ACCAGAAAAA GACCCAGCTT 
GAGCATATTC TTCTGCGACC TGACACATAC ATTGGATCTG TGGAGACAGT TACTCAGTCC 
ATGTGGGTTT ATGATGAAGA TGTTGGAATG AATTGCCGTG ACATAACCTA TGTGCCCGGT 
TTATACAAGA TATTTGATGA AATCCTTGTA AACGCAGCTG ATAATAAACA GAGAGATAAA 
AATATGAACT GCATAAAAAT TTCTATTGAT CCTGATTCTA ACATCATCAG CATTTGGAAT 
AATGGTAAAG GAATACCTGT GGTTGAGCAC AAAGTGGAGA AAATGTATGT CCCTGCTTTA 
ATTTTTGGCC AGTTATTAAC ATCAAGCAAC TATGATGATG AGGAAAAGAA AGTTACAGGA 
GGGCGAAATG GATATGGTGC TAAACTTTGC AATATATTCA GCACAAAATT TACTGTGGAG 
ACAGCTTGTA AGGAATACAA GCATAGCTTT AAGCAGACAT GGACAAACAA CATGTTAAAG 
ACAACTGAAC CCAAAATTAA ACATTTTGAT GGAGATGACT ACACATGCAT AACATTCCAG 
CCAGATTTAG CCAAGTTCCA AATGGAAAAA CTGGACAAGG ATACTGTGGC TCTTCTGACA 
AGACGAGCAT ATGATATTGC GGGTTCCTGC AAAGGAGTCA AAGTCTTGCT CAATGGGAAG 
AAGCTTCCGG TAAATGGCTT CCGCAGTTAC GTAGATCTTT ACGTAAAGGA CAGACTTGAT 
GAAACTGGTG TCGCTCTTAA AGTAATCCAT GAAGTTGCAA ATGAAAGATG GGATGTATGT 
TTAACATTGA GCGAAAAGGG ATTCCAACAA ATCAGTTTTG TGAACAGTAT TGCTACCACA 
AAGGGTGGTA GACATGTAGA TTATGTAGTA GACCAAATTG TGTCAAAACT CATTGAAGTT 
GTGAAGAAGA AGAATAAAGC TGGAGTTTCT GTAAAGCCCT TCCAGGTGAA GAATCACGTT 
TGGGTTTTTG TCAACTGTTT AATAGAAAAC CCAACTTTTG ATTCTCAGAC AAAAGAAAAC 
ATGACACTAC AGCCTAAAGG TTTTGGGTCC AAATGCCAGC TGTCAGAAAA ATTTTCAAAA 
GCGGCATCTA ACTGTGGCAT AGTCGAGAGT ATTCTAAATT GGGTTAAATT CAAGGCCCAA 
ACACAGCTAA ATAAGAAATG CTCTTCTGTG AAACACAGTA AAATCAAAGG CATTCCCAAG 
CTGGATGATG CTAATGATGC AGGCGGAAAA CATTCCCTGG ACTGCACTCT GATATTAACT 
GAGGGAGACT CTGCCAAATC TTTAGCTGTT TCAGGCTTAG GTGTCATTGG CCGAGATCGG 
TACGGTGTAT TCCCGCTCAG GGGAAAAATT CTCAATGTTC GAGAAGCTTC CCACAAACAG 
ATTATGGAAA ATGCTGAGAT AAACAACATC ATTAAAATAG TTGGCTTGCA GTACAAAAAA 
AGCTATGATG ATAATGAATC CCTTAAAACC TTGCGTTATG GAAAAATTAT GATAATGACA 
GATCAGGATC AAGATGGCTC GCATATCAAG GGCTTATTGA TTAATTTTTT CCATCACAAT 
TGGCCATCTC TACTGAAACA TAGTTTCCTT GAAGAATTTA TCACACCCAT TGTTAAGGCT 
ACAAAGAATA AGCAGGAGCT TTCATTCTAC AGTATTCCTG AGTTTGAAGA ATGGAAAAAA 
CAAACAGAAA ACCAAAAATT GTGGAAGATT AAGTATTACA AAGGTTTGGG CACCAGCACT 
TCGAAGGAAG CAAAGGAATA CTTTGCTGAC ATGGACAGGC ACAGAATTAT ATTTAGATAT 
GCAGGCCCTG AAGATGATGC AGCTATCACT CTGGCTTTCA GCAAAAAGAA AGTAGATGAC 
AGGAAAGAAT GGCTCACAAA CTTTATGGAA GACAGGCGAC AACGTCGACT ACACGGATTG 
CCCGAGCAAT TCTTGTACGG CACAGCTACA AAACACTTGA CTTACAATGA CTTCATTAAC 
AAGGAGCTGA TTCTTTTTTC AAACTCTGAT AATGAGCGAT CTATTCCATC TCTTGTTGAT 
GGTTTAAAGC CTGGGCAAAG AAAAGTTCTG TTTACTTGTT TTAAAAGGAA TGATAAACGG 
GAAGTCAAGG TTGCTCAGCT GGCTGGTTCT GTTGCTGAAA TGTCCGCTTA CCACCATGGA 
GAGCAAGCCT TGATGATGAC AATAGTAAAT CTTGCCCAGA ACTTTGTGGG AAGTAACAAT 
GTGAGCTTGC TGCAGCCCAT TGGTCAGTTT GGTACTCGAC TCCATGGTGG AAAGGATGCT 
GCCAGCCCTC GTTATATATT CACAATGTTA AGTCCTCTAG CAAGATTGCT CTATCCATCT 
GTGGATGATA ACCTTTTGAA GTTCCTTTAT GATGATAACC AGCGAGTAGA ACCAGAGTGG 
TACATTCCTA TTATACCCCT GGTTTTAGTT AATGGAGCAG AAGGAATCGG TACTGGATGG 
GCTTGTAAAC TGCCCAATTA TGACACAAGA GAAATTGTCA ATAATGTGAA GAGAATGCTG 
GATGGTCTTG ATCCTTATCC ATTGCTTCCA AGCTACAAGA ATTTTAAAGG TACGATTCAG 
GAACTGGGAC AGAATCAGTA TGCAATGAGT GGTGAAATAT ATGTGCTGGA CCGGAACACA 
ATTGAAATCA CAGAACTTCC TGTCAGGACA TGGACCCAGG TTTACAAAGA ACAAGTGCTG 
GAGCCCATGC TCAATGGAAC CGAGAAAACA CCATCGTTAA TCTCTGATTA CAAAGAGTAT 
CATACAGACA CAACTGTAAA ATTTGTTGTC AAAATGACAG AAGAAAAGTT GGCACAGGCA 
GAAGCTGCTG GCTTGCATAA AGTCTTTAAA CTTCAAACAA GTCTTACTTG CAATTCTATG 
GTATTATTTG ACCACATGGG CTGTCTAAAG AAATATGAAA CCGTGCAGGA CATTCTGAAG 
GAATTTTTTG ATCTCCGGTT AAATTACTAC ACCCTGCGCA AAGAATGGCT GATAGGAATG 
TTGGGAGCTG AAGCTGCAAG ACTGAACAAC CAAGCCCGGT TTGTCCTGGA GAAGATACAA 
GGCAAAATCT CTATAGAGAA CAAAACCAAA AGAGATCTAA TTCAGATGTT GGTGCAGAAA 
GGATTTGAGT CAGACCCTGT GAAAGCTTGG AAGGAGGCAC AAGAAAAAGC AACAGAGGAG 
GATGACCTTC AAGATCCGAA TGAAGACAAT TCATCCGACT CTGGCACTGG ATCAGGACCG 
GATTTTAACT ACATTCTCAA TATGTCTCTC TGGAGCCTTT CAAAGGAAAA AGTAGATGAG 
CTTGTCAGAC AGAGGGATAT AAAGAGCAAA GAACTGAATG ACCTCAAGAG GAAGTCTCCG 
CCTGATCTGT GGAAGGAAGA CTTGGCAGCT TTTGTTGAAG AGCTAGACAG AGTGGAAGCT 
CAAGAAAAAG AAGATGTCTT AGCAGGGTTT GCAGGCAAAG CAATAAAAGG CAAAATTGGA 
AAACCCAAAA TGAAGAAATT ACAGCTTGAG GAGACTTTGC CATCTCTTTA CGGCAGAAGA 
GTAGAGCCAC AAATCAGTGC AATGAAAGCA GATGCCAGCA AAAAGATGCT AAAGAAAAAG 
AAGGGTGAAA TTGACTCTAT TGCACTGAAA ATGGAATTTG ATGAGGAGTT TAGCTCGGCA 
CCAGCAGAGG GAGGAGCTGA AGATTCCATG AATACCACTC CTGTAAAACC TGTAAAGCCC 
AAGAGAGAAA GAAAAGAACC AGGTCCTCGA GCCAAAAAGA CTGTATTAAC ACCCAAGTCA 
TCAACCAAGA AAGGGAAGAA GCGAAACCCT TGGTCAGACA ATGAGTCAAA ATCAGACAGT 
GACCTGGAAG AAGCTGAGGC AGAGCCTGTG GTCATTCCTC GGGACTCTCT CCTAAGGAGA 
GCAGCTGCTG AAAGACCGAA GTACACGTTT GATTTCTCTG AAGAGGAAGA TGCAGACGAT 
GACGACGAAG GTGTCATTAG CCGTAGTAAT AATAATAATA ATATTAATGA CTTAAAACCG 
CCAGCTTCTC CTGCTGCTAA TAGCCGAGGT GAAGATGATG ATGTTCTCTC TGACAGCCAA 
GAAAAGGATG ACTATGAATT CTCCCCTTTC AAGTCTACCC CAGCCGCACA AAAGCCTTCA 
GAAACACAAA AGAAAAAAGA CTACATATTT TCATCATCTT CATTTTCAGA AAAAGTAGAA 
GATTCTGCAA TATATAACAG TGACGATGAT GAACCCTCCC CACTTTACTC TTCGTACTCC 
TTCAAAACTT CAGAGAAAAC AACAGGCAAA GGATCTAGTA AAAAAGAATC TGCAAAATAT 
GACAGTGATG ATGATGATGA TGATGAACCC TCCCCCCTTT ACTCCTCATA TTCCTTCAAA 
TCTACAGAAA AGCCAGCCAG CAAAGGATCT AGTGAGAAAG AATCCACCAA ATATAACAGT 
GACGATGAGG ATGATGACGA TGACGATGTA CCGTTTTCGT CGTTTACCTT TAAATCTTCC 
GAAAAGCCAG CCAGTAAAGG TTCTGCTAAG AAAACAAAAT CTTTGTCAGA TGGTACTCCT 
AAACCAAAAC GACCGCCAAA ACCAAAGAAG TCTGAGACTT CATTGATTTC CGACTCTGAC 
TTGTTAGATT TTGGTGCATC AAAGAAATCT GCAACACCCA AAGCAAAAGG AAGGGGAAGG 
AAACGAAAAA CATCTGATTC TGAAAATGAA GGAGATTACA ATCCAGGGCG AAAACCTACA 
AAACCTGCAT CTTCTAGCAA AAAATCCAAG AAAGCATCAT TTGAGCAGGA TTCAGATGGT 
GATCTCTTCC CTTCAGAAGT CGGCTCTGAG ACCGCATCGA GGCAAAGGCC TGGCCGGGCT 
AGGAAAGAAG TCAAATATTT TGCAGACTCT GACGATGAAG ATGATTTTGC TATGTTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_002932456.3
CDS	872..5971
Translation	MSKSGGGGGGGAGGGSGGLTWVTLFDNQNASKKEETESNNKNDSSKKMSVERVYQKKTQLEHILLRPDTYIGSVETVTQSMWVYDEDVGMNCRDITYVPGLYKIFDEILVNAADNKQRDKNMNCIKISIDPDSNIISIWNNGKGIPVVEHKVEKMYVPALIFGQLLTSSNYDDEEKKVTGGRNGYGAKLCNIFSTKFTVETACKEYKHSFKQTWTNNMLKTTEPKIKHFDGDDYTCITFQPDLAKFQMEKLDKDTVALLTRRAYDIAGSCKGVKVLLNGKKLPVNGFRSYVDLYVKDRLDETGVALKVIHEVANERWDVCLTLSEKGFQQISFVNSIATTKGGRHVDYVVDQIVSKLIEVVKKKNKAGVSVKPFQVKNHVWVFVNCLIENPTFDSQTKENMTLQPKGFGSKCQLSEKFSKAASNCGIVESILNWVKFKAQTQLNKKCSSVKHSKIKGIPKLDDANDAGGKHSLDCTLILTEGDSAKSLAVSGLGVIGRDRYGVFPLRGKILNVREASHKQIMENAEINNIIKIVGLQYKKSYDDNESLKTLRYGKIMIMTDQDQDGSHIKGLLINFFHHNWPSLLKHSFLEEFITPIVKATKNKQELSFYSIPEFEEWKKQTENQKLWKIKYYKGLGTSTSKEAKEYFADMDRHRIIFRYAGPEDDAAITLAFSKKKVDDRKEWLTNFMEDRRQRRLHGLPEQFLYGTATKHLTYNDFINKELILFSNSDNERSIPSLVDGLKPGQRKVLFTCFKRNDKREVKVAQLAGSVAEMSAYHHGEQALMMTIVNLAQNFVGSNNVSLLQPIGQFGTRLHGGKDAASPRYIFTMLSPLARLLYPSVDDNLLKFLYDDNQRVEPEWYIPIIPLVLVNGAEGIGTGWACKLPNYDTREIVNNVKRMLDGLDPYPLLPSYKNFKGTIQELGQNQYAMSGEIYVLDRNTIEITELPVRTWTQVYKEQVLEPMLNGTEKTPSLISDYKEYHTDTTVKFVVKMTEEKLAQAEAAGLHKVFKLQTSLTCNSMVLFDHMGCLKKYETVQDILKEFFDLRLNYYTLRKEWLIGMLGAEAARLNNQARFVLEKIQGKISIENKTKRDLIQMLVQKGFESDPVKAWKEAQEKATEEDDLQDPNEDNSSDSGTGSGPDFNYILNMSLWSLSKEKVDELVRQRDIKSKELNDLKRKSPPDLWKEDLAAFVEELDRVEAQEKEDVLAGFAGKAIKGKIGKPKMKKLQLEETLPSLYGRRVEPQISAMKADASKKMLKKKKGEIDSIALKMEFDEEFSSAPAEGGAEDSMNTTPVKPVKPKRERKEPGPRAKKTVLTPKSSTKKGKKRNPWSDNESKSDSDLEEAEAEPVVIPRDSLLRRAAAERPKYTFDFSEEEDADDDDEGVISRSNNNNNINDLKPPASPAANSRGEDDDVLSDSQEKDDYEFSPFKSTPAAQKPSETQKKKDYIFSSSSFSEKVEDSAIYNSDDDEPSPLYSSYSFKTSEKTTGKGSSKKESAKYDSDDDDDDEPSPLYSSYSFKSTEKPASKGSSEKESTKYNSDDEDDDDDDVPFSSFTFKSSEKPASKGSAKKTKSLSDGTPKPKRPPKPKKSETSLISDSDLLDFGASKKSATPKAKGRGRKRKTSDSENEGDYNPGRKPTKPASSSKKSKKASFEQDSDGDLFPSEVGSETASRQRPGRARKEVKYFADSDDEDDFAMF

Target ORF information:

RefSeq Version	XM_002932410.5
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis DNA topoisomerase II beta (top2b), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_002932410.5

ORF Insert Sequence:

ATGTCTAAAT CTGGCGGCGG CGGCGGCGGA GGAGCTGGAG GAGGGAGCGG AGGGCTCACC 
TGGGTGACAC TTTTTGATAA TCAGAACGCA TCAAAAAAGG AAGAAACAGA AAGCAATAAT 
AAAAATGACT CCTCCAAGAA GATGTCCGTA GAAAGAGTTT ACCAGAAAAA GACCCAGCTT 
GAGCATATTC TTCTGCGACC TGACACATAC ATTGGATCTG TGGAGACAGT TACTCAGTCC 
ATGTGGGTTT ATGATGAAGA TGTTGGAATG AATTGCCGTG ACATAACCTA TGTGCCCGGT 
TTATACAAGA TATTTGATGA AATCCTTGTA AACGCAGCTG ATAATAAACA GAGAGATAAA 
AATATGAACT GCATAAAAAT TTCTATTGAT CCTGATTCTA ACATCATCAG CATTTGGAAT 
AATGGTAAAG GAATACCTGT GGTTGAGCAC AAAGTGGAGA AAATGTATGT CCCTGCTTTA 
ATTTTTGGCC AGTTATTAAC ATCAAGCAAC TATGATGATG AGGAAAAGAA AGTTACAGGA 
GGGCGAAATG GATATGGTGC TAAACTTTGC AATATATTCA GCACAAAATT TACTGTGGAG 
ACAGCTTGTA AGGAATACAA GCATAGCTTT AAGCAGACAT GGACAAACAA CATGTTAAAG 
ACAACTGAAC CCAAAATTAA ACATTTTGAT GGAGATGACT ACACATGCAT AACATTCCAG 
CCAGATTTAG CCAAGTTCCA AATGGAAAAA CTGGACAAGG ATACTGTGGC TCTTCTGACA 
AGACGAGCAT ATGATATTGC GGGTTCCTGC AAAGGAGTCA AAGTCTTGCT CAATGGGAAG 
AAGCTTCCGG TAAATGGCTT CCGCAGTTAC GTAGATCTTT ACGTAAAGGA CAGACTTGAT 
GAAACTGGTG TCGCTCTTAA AGTAATCCAT GAAGTTGCAA ATGAAAGATG GGATGTATGT 
TTAACATTGA GCGAAAAGGG ATTCCAACAA ATCAGTTTTG TGAACAGTAT TGCTACCACA 
AAGGGTGGTA GACATGTAGA TTATGTAGTA GACCAAATTG TGTCAAAACT CATTGAAGTT 
GTGAAGAAGA AGAATAAAGC TGGAGTTTCT GTAAAGCCCT TCCAGGTGAA GAATCACGTT 
TGGGTTTTTG TCAACTGTTT AATAGAAAAC CCAACTTTTG ATTCTCAGAC AAAAGAAAAC 
ATGACACTAC AGCCTAAAGG TTTTGGGTCC AAATGCCAGC TGTCAGAAAA ATTTTCAAAA 
GCGGCATCTA ACTGTGGCAT AGTCGAGAGT ATTCTAAATT GGGTTAAATT CAAGGCCCAA 
ACACAGCTAA ATAAGAAATG CTCTTCTGTG AAACACAGTA AAATCAAAGG CATTCCCAAG 
CTGGATGATG CTAATGATGC AGGCGGAAAA CATTCCCTGG ACTGCACTCT GATATTAACT 
GAGGGAGACT CTGCCAAATC TTTAGCTGTT TCAGGCTTAG GTGTCATTGG CCGAGATCGG 
TACGGTGTAT TCCCGCTCAG GGGAAAAATT CTCAATGTTC GAGAAGCTTC CCACAAACAG 
ATTATGGAAA ATGCTGAGAT AAACAACATC ATTAAAATAG TTGGCTTGCA GTACAAAAAA 
AGCTATGATG ATAATGAATC CCTTAAAACC TTGCGTTATG GAAAAATTAT GATAATGACA 
GATCAGGATC AAGATGGCTC GCATATCAAG GGCTTATTGA TTAATTTTTT CCATCACAAT 
TGGCCATCTC TACTGAAACA TAGTTTCCTT GAAGAATTTA TCACACCCAT TGTTAAGGCT 
ACAAAGAATA AGCAGGAGCT TTCATTCTAC AGTATTCCTG AGTTTGAAGA ATGGAAAAAA 
CAAACAGAAA ACCAAAAATT GTGGAAGATT AAGTATTACA AAGGTTTGGG CACCAGCACT 
TCGAAGGAAG CAAAGGAATA CTTTGCTGAC ATGGACAGGC ACAGAATTAT ATTTAGATAT 
GCAGGCCCTG AAGATGATGC AGCTATCACT CTGGCTTTCA GCAAAAAGAA AGTAGATGAC 
AGGAAAGAAT GGCTCACAAA CTTTATGGAA GACAGGCGAC AACGTCGACT ACACGGATTG 
CCCGAGCAAT TCTTGTACGG CACAGCTACA AAACACTTGA CTTACAATGA CTTCATTAAC 
AAGGAGCTGA TTCTTTTTTC AAACTCTGAT AATGAGCGAT CTATTCCATC TCTTGTTGAT 
GGTTTAAAGC CTGGGCAAAG AAAAGTTCTG TTTACTTGTT TTAAAAGGAA TGATAAACGG 
GAAGTCAAGG TTGCTCAGCT GGCTGGTTCT GTTGCTGAAA TGTCCGCTTA CCACCATGGA 
GAGCAAGCCT TGATGATGAC AATAGTAAAT CTTGCCCAGA ACTTTGTGGG AAGTAACAAT 
GTGAGCTTGC TGCAGCCCAT TGGTCAGTTT GGTACTCGAC TCCATGGTGG AAAGGATGCT 
GCCAGCCCTC GTTATATATT CACAATGTTA AGTCCTCTAG CAAGATTGCT CTATCCATCT 
GTGGATGATA ACCTTTTGAA GTTCCTTTAT GATGATAACC AGCGAGTAGA ACCAGAGTGG 
TACATTCCTA TTATACCCCT GGTTTTAGTT AATGGAGCAG AAGGAATCGG TACTGGATGG 
GCTTGTAAAC TGCCCAATTA TGACACAAGA GAAATTGTCA ATAATGTGAA GAGAATGCTG 
GATGGTCTTG ATCCTTATCC ATTGCTTCCA AGCTACAAGA ATTTTAAAGG TACGATTCAG 
GAACTGGGAC AGAATCAGTA TGCAATGAGT GGTGAAATAT ATGTGCTGGA CCGGAACACA 
ATTGAAATCA CAGAACTTCC TGTCAGGACA TGGACCCAGG TTTACAAAGA ACAAGTGCTG 
GAGCCCATGC TCAATGGAAC CGAGAAAACA CCATCGTTAA TCTCTGATTA CAAAGAGTAT 
CATACAGACA CAACTGTAAA ATTTGTTGTC AAAATGACAG AAGAAAAGTT GGCACAGGCA 
GAAGCTGCTG GCTTGCATAA AGTCTTTAAA CTTCAAACAA GTCTTACTTG CAATTCTATG 
GTATTATTTG ACCACATGGG CTGTCTAAAG AAATATGAAA CCGTGCAGGA CATTCTGAAG 
GAATTTTTTG ATCTCCGGTT AAATTACTAC ACCCTGCGCA AAGAATGGCT GATAGGAATG 
TTGGGAGCTG AAGCTGCAAG ACTGAACAAC CAAGCCCGGT TTGTCCTGGA GAAGATACAA 
GGCAAAATCT CTATAGAGAA CAAAACCAAA AGAGATCTAA TTCAGATGTT GGTGCAGAAA 
GGATTTGAGT CAGACCCTGT GAAAGCTTGG AAGGAGGCAC AAGAAAAAGC AACAGAGGAG 
GATGACCTTC AAGATCCGAA TGAAGACAAT TCATCCGACT CTGGCACTGG ATCAGGACCG 
GATTTTAACT ACATTCTCAA TATGTCTCTC TGGAGCCTTT CAAAGGAAAA AGTAGATGAG 
CTTGTCAGAC AGAGGGATAT AAAGAGCAAA GAACTGAATG ACCTCAAGAG GAAGTCTCCG 
CCTGATCTGT GGAAGGAAGA CTTGGCAGCT TTTGTTGAAG AGCTAGACAG AGTGGAAGCT 
CAAGAAAAAG AAGATGTCTT AGCAGGGTTT GCAGGCAAAG CAATAAAAGG CAAAATTGGA 
AAACCCAAAA TGAAGAAATT ACAGCTTGAG GAGACTTTGC CATCTCTTTA CGGCAGAAGA 
GTAGAGCCAC AAATCAGTGC AATGAAAGCA GATGCCAGCA AAAAGATGCT AAAGAAAAAG 
AAGGGTGAAA TTGACTCTAT TGCACTGAAA ATGGAATTTG ATGAGGAGTT TAGCTCGGCA 
CCAGCAGAGG GAGGAGCTGA AGATTCCATG AATACCACTC CTGTAAAACC TGTAAAGCCC 
AAGAGAGAAA GAAAAGAACC AGGTCCTCGA GCCAAAAAGA CTGTATTAAC ACCCAAGTCA 
TCAACCAAGA AAGGGAAGAA GCGAAACCCT TGGTCAGACA ATGAGTCAAA ATCAGACAGT 
GACCTGGAAG AAGCTGAGGC AGAGCCTGTG GTCATTCCTC GGGACTCTCT CCTAAGGAGA 
GCAGCTGCTG AAAGACCGAA GTACACGTTT GATTTCTCTG AAGAGGAAGA TGCAGACGAT 
GACGACGAAG GTGTCATTAG CCGTAGTAAT AATAATAATA ATATTAATGA CTTAAAACCG 
CCAGCTTCTC CTGCTGCTAA TAGCCGAGGT GAAGATGATG ATGTTCTCTC TGACAGCCAA 
GAAAAGGATG ACTATGAATT CTCCCCTTTC AAGTCTACCC CAGCCGCACA AAAGCCTTCA 
GAAACACAAA AGAAAAAAGA CTACATATTT TCATCATCTT CATTTTCAGA AAAAGTAGAA 
GATTCTGCAA TATATAACAG TGACGATGAT GAACCCTCCC CACTTTACTC TTCGTACTCC 
TTCAAAACTT CAGAGAAAAC AACAGGCAAA GGATCTAGTA AAAAAGAATC TGCAAAATAT 
GACAGTGATG ATGATGATGA TGATGAACCC TCCCCCCTTT ACTCCTCATA TTCCTTCAAA 
TCTACAGAAA AGCCAGCCAG CAAAGGATCT AGTGAGAAAG AATCCACCAA ATATAACAGT 
GACGATGAGG ATGATGACGA TGACGATGTA CCGTTTTCGT CGTTTACCTT TAAATCTTCC 
GAAAAGCCAG CCAGTAAAGG TTCTGCTAAG AAAACAAAAT CTTTGTCAGA TGGTACTCCT 
AAACCAAAAC GACCGCCAAA ACCAAAGAAG TCTGAGACTT CATTGATTTC CGACTCTGAC 
TTGTTAGATT TTGGTGCATC AAAGAAATCT GCAACACCCA AAGCAAAAGG AAGGGGAAGG 
AAACGAAAAA CATCTGATTC TGAAAATGAA GGAGATTACA ATCCAGGGCG AAAACCTACA 
AAACCTGCAT CTTCTAGCAA AAAATCCAAG AAAGCATCAT TTGAGCAGGA TTCAGATGGT 
GATCTCTTCC CTTCAGAAGT CGGCTCTGAG ACCGCATCGA GGCAAAGGCC TGGCCGGGCT 
AGGAAAGAAG TCAAATATTT TGCAGACTCT GACGATGAAG ATGATTTTGC TATGTTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

top2b ( XM_018095006.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_017950495.2 Xenopus tropicalis DNA topoisomerase II beta (top2b), transcript variant X2, mRNA.

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CloneID	OXa60893
Clone ID Related Accession (Same CDS sequence)	XM_018095006.2
Accession Version	XM_018095006.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4995bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1576512000000
Organism	Xenopus tropicalis(tropical clawed frog)
Product	DNA topoisomerase 2-beta isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030682.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_018095006.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTAAAT CTGGCGGCGG CGGCGGCGGA GGAGCTGGAG GAGGGAGCGG AGGGCTCACC 
TGGGTGACAC TTTTTGATAA TCAGAACGCA TCAAAAAAGG AAGAAACAGA AAGCAATAAT 
AAAAATGACT CCTCCAAGAA GATGTCCGTA GAAAGAGTTT ACCAGAAAAA GACCCAGCTT 
GAGCATATTC TTCTGCGACC TGACACATAC ATTGGATCTG TGGAGACAGT TACTCAGTCC 
ATGTGGGTTT ATGATGAAGA TGTTGGAATG AATTGCCGTG ACATAACCTA TGTGCCCGGT 
TTATACAAGA TATTTGATGA AATCCTTGTA AACGCAGCTG ATAATAAACA GAGAGATAAA 
AATATGAACT GCATAAAAAT TTCTATTGAT CCTGATTCTA ACATCATCAG CATTTGGAAT 
AATGGTAAAG GAATACCTGT GGTTGAGCAC AAAGTGGAGA AAATGTATGT CCCTGCTTTA 
ATTTTTGGCC AGTTATTAAC ATCAAGCAAC TATGATGATG AGGAAAAGAA AGTTACAGGA 
GGGCGAAATG GATATGGTGC TAAACTTTGC AATATATTCA GCACAAAATT TACTGTGGAG 
ACAGCTTGTA AGGAATACAA GCATAGCTTT AAGCAGACAT GGACAAACAA CATGTTAAAG 
ACAACTGAAC CCAAAATTAA ACATTTTGAT GGAGATGACT ACACATGCAT AACATTCCAG 
CCAGATTTAG CCAAGTTCCA AATGGAAAAA CTGGACAAGG ATACTGTGGC TCTTCTGACA 
AGACGAGCAT ATGATATTGC GGGTTCCTGC AAAGGAGTCA AAGTCTTGCT CAATGGGAAG 
AAGCTTCCGG TAAATGGCTT CCGCAGTTAC GTAGATCTTT ACGTAAAGGA CAGACTTGAT 
GAAACTGGTG TCGCTCTTAA AGTAATCCAT GAAGTTGCAA ATGAAAGATG GGATGTATGT 
TTAACATTGA GCGAAAAGGG ATTCCAACAA ATCAGTTTTG TGAACAGTAT TGCTACCACA 
AAGGGTGGTA GACATGTAGA TTATGTAGTA GACCAAATTG TGTCAAAACT CATTGAAGTT 
GTGAAGAAGA AGAATAAAGC TGGAGTTTCT GTAAAGCCCT TCCAGGTGAA GAATCACGTT 
TGGGTTTTTG TCAACTGTTT AATAGAAAAC CCAACTTTTG ATTCTCAGAC AAAAGAAAAC 
ATGACACTAC AGCCTAAAGG TTTTGGGTCC AAATGCCAGC TGTCAGAAAA ATTTTCAAAA 
GCGGCATCTA ACTGTGGCAT AGTCGAGAGT ATTCTAAATT GGGTTAAATT CAAGGCCCAA 
ACACAGCTAA ATAAGAAATG CTCTTCTGTG AAACACAGTA AAATCAAAGG CATTCCCAAG 
CTGGATGATG CTAATGATGC AGGCGGAAAA CATTCCCTGG ACTGCACTCT GATATTAACT 
GAGGGAGACT CTGCCAAATC TTTAGCTGTT TCAGGCTTAG GTGTCATTGG CCGAGATCGG 
TACGGTGTAT TCCCGCTCAG GGGAAAAATT CTCAATGTTC GAGAAGCTTC CCACAAACAG 
ATTATGGAAA ATGCTGAGAT AAACAACATC ATTAAAATAG TTGGCTTGCA GTACAAAAAA 
AGCTATGATG ATAATGAATC CCTTAAAACC TTGCGTTATG GAAAAATTAT GATAATGACA 
GATCAGGATC AAGATGGCTC GCATATCAAG GGCTTATTGA TTAATTTTTT CCATCACAAT 
TGGCCATCTC TACTGAAACA TAGTTTCCTT GAAGAATTTA TCACACCCAT TGTTAAGGCT 
ACAAAGAATA AGCAGGAGCT TTCATTCTAC AGTATTCCTG AGTTTGAAGA ATGGAAAAAA 
CAAACAGAAA ACCAAAAATT GTGGAAGATT AAGTATTACA AAGGTTTGGG CACCAGCACT 
TCGAAGGAAG CAAAGGAATA CTTTGCTGAC ATGGACAGGC ACAGAATTAT ATTTAGATAT 
GCAGGCCCTG AAGATGATGC AGCTATCACT CTGGCTTTCA GCAAAAAGAA AGTAGATGAC 
AGGAAAGAAT GGCTCACAAA CTTTATGGAA GACAGGCGAC AACGTCGACT ACACGGATTG 
CCCGAGCAAT TCTTGTACGG CACAGCTACA AAACACTTGA CTTACAATGA CTTCATTAAC 
AAGGAGCTGA TTCTTTTTTC AAACTCTGAT AATGAGCGAT CTATTCCATC TCTTGTTGAT 
GGTTTAAAGC CTGGGCAAAG AAAAGTTCTG TTTACTTGTT TTAAAAGGAA TGATAAACGG 
GAAGTCAAGG TTGCTCAGCT GGCTGGTTCT GTTGCTGAAA TGTCCGCTTA CCACCATGGA 
GAGCAAGCCT TGATGATGAC AATAGTAAAT CTTGCCCAGA ACTTTGTGGG AAGTAACAAT 
GTGAGCTTGC TGCAGCCCAT TGGTCAGTTT GGTACTCGAC TCCATGGTGG AAAGGATGCT 
GCCAGCCCTC GTTATATATT CACAATGTTA AGTCCTCTAG CAAGATTGCT CTATCCATCT 
GTGGATGATA ACCTTTTGAA GTTCCTTTAT GATGATAACC AGCGAGTAGA ACCAGAGTGG 
TACATTCCTA TTATACCCCT GGTTTTAGTT AATGGAGCAG AAGGAATCGG TACTGGATGG 
GCTTGTAAAC TGCCCAATTA TGACACAAGA GAAATTGTCA ATAATGTGAA GAGAATGCTG 
GATGGTCTTG ATCCTTATCC ATTGCTTCCA AGCTACAAGA ATTTTAAAGG TACGATTCAG 
GAACTGGGAC AGAATCAGTA TGCAATGAGT GGTGAAATAT ATGTGCTGGA CCGGAACACA 
ATTGAAATCA CAGAACTTCC TGTCAGGACA TGGACCCAGG TTTACAAAGA ACAAGTGCTG 
GAGCCCATGC TCAATGGAAC CGAGAAAACA CCATCGTTAA TCTCTGATTA CAAAGAGTAT 
CATACAGACA CAACTGTAAA ATTTGTTGTC AAAATGACAG AAGAAAAGTT GGCACAGGCA 
GAAGCTGCTG GCTTGCATAA AGTCTTTAAA CTTCAAACAA GTCTTACTTG CAATTCTATG 
GTATTATTTG ACCACATGGG CTGTCTAAAG AAATATGAAA CCGTGCAGGA CATTCTGAAG 
GAATTTTTTG ATCTCCGGTT AAATTACTAC ACCCTGCGCA AAGAATGGCT GATAGGAATG 
TTGGGAGCTG AAGCTGCAAG ACTGAACAAC CAAGCCCGGT TTGTCCTGGA GAAGATACAA 
GGCAAAATCT CTATAGAGAA CAAAACCAAA AGAGATCTAA TTCAGATGTT GGTGCAGAAA 
GGATTTGAGT CAGACCCTGT GAAAGCTTGG AAGGAGGCAC AAGAAAAAGC AACAGAGGAG 
GATGACCTTC AAGATCCGAA TGAAGACAAT TCATCCGACT CTGGCACTGG ATCAGGACCG 
GATTTTAACT ACATTCTCAA TATGTCTCTC TGGAGCCTTT CAAAGGAAAA AGTAGATGAG 
CTTGTCAGAC AGAGGGATAT AAAGAGCAAA GAACTGAATG ACCTCAAGAG GAAGTCTCCG 
CCTGATCTGT GGAAGGAAGA CTTGGCAGCT TTTGTTGAAG AGCTAGACAG AGTGGAAGCT 
CAAGAAAAAG AAGATGTCTT AGCAGGGTTT GCAGGCAAAG CAATAAAAGG CAAAATTGGA 
AAACCCAAAA TGAAGAAATT ACAGCTTGAG GAGACTTTGC CATCTCTTTA CGGCAGAAGA 
GTAGAGCCAC AAATCAGTGC AATGAAAGCA GATGCCAGCA AAAAGATGCT AAAGAAAAAG 
AAGGGTGAAA TTGACTCTAT TGCACTGAAA ATGGAATTTG ATGAGGAGTT TAGCTCGGCA 
CCAGCAGAGG GAGGAGCTGA AGATTCCATG AATACCACTC CTGTAAAACC TGTAAAGCCC 
AAGAGAGAAA GAAAAGAACC AGGTCCTCGA GCCAAAAAGA CTGTATTAAC ACCCAAGTCA 
TCAACCAAGA AAGGGAAGAA GCGAAACCCT TGGTCAGACA ATGAGTCAAA ATCAGACAGT 
GACCTGGAAG AAGCTGAGGC AGAGCCTGTG GTCATTCCTC GGGACTCTCT CCTAAGGAGA 
GCAGCTGCTG AAAGACCGAA GTACACGTTT GATTTCTCTG AAGAGGAAGA TGCAGACGAT 
GACGACGAAG GTGTCATTAG CCGTAGTAAT AATAATAATA ATATTAATGA CTTAAAACCG 
CCAGCTTCTC CTGCTGCTAA TAGCCGAGGT GAAGATGATG ATGTTCTCTC TGACAGCCAA 
GAAAAGGATG ACTATGAATT CTCCCCTTTC AAGTCTACCC CAGCCGCACA AAAGCCTTCA 
GAAACACAAA AGAAAAAAGA CTACATATTT TCATCATCTT CATTTTCAGA AAAAGTAGAA 
GAATCTGCAA AATATGACAG TGATGATGAT GATGATGATG AACCCTCCCC CCTTTACTCC 
TCATATTCCT TCAAATCTAC AGAAAAGCCA GCCAGCAAAG GATCTAGTGA GAAAGAATCC 
ACCAAATATA ACAGTGACGA TGAGGATGAT GACGATGACG ATGTACCGTT TTCGTCGTTT 
ACCTTTAAAT CTTCCGAAAA GCCAGCCAGT AAAGGTTCTG CTAAGAAAAC AAAATCTTTG 
TCAGATGGTA CTCCTAAACC AAAACGACCG CCAAAACCAA AGAAGTCTGA GACTTCATTG 
ATTTCCGACT CTGACTTGTT AGATTTTGGT GCATCAAAGA AATCTGCAAC ACCCAAAGCA 
AAAGGAAGGG GAAGGAAACG AAAAACATCT GATTCTGAAA ATGAAGGAGA TTACAATCCA 
GGGCGAAAAC CTACAAAACC TGCATCTTCT AGCAAAAAAT CCAAGAAAGC ATCATTTGAG 
CAGGATTCAG ATGGTGATCT CTTCCCTTCA GAAGTCGGCT CTGAGACCGC ATCGAGGCAA 
AGGCCTGGCC GGGCTAGGAA AGAAGTCAAA TATTTTGCAG ACTCTGACGA TGAAGATGAT 
TTTGCTATGT TTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017950495.2
CDS	872..5866
Translation	MSKSGGGGGGGAGGGSGGLTWVTLFDNQNASKKEETESNNKNDSSKKMSVERVYQKKTQLEHILLRPDTYIGSVETVTQSMWVYDEDVGMNCRDITYVPGLYKIFDEILVNAADNKQRDKNMNCIKISIDPDSNIISIWNNGKGIPVVEHKVEKMYVPALIFGQLLTSSNYDDEEKKVTGGRNGYGAKLCNIFSTKFTVETACKEYKHSFKQTWTNNMLKTTEPKIKHFDGDDYTCITFQPDLAKFQMEKLDKDTVALLTRRAYDIAGSCKGVKVLLNGKKLPVNGFRSYVDLYVKDRLDETGVALKVIHEVANERWDVCLTLSEKGFQQISFVNSIATTKGGRHVDYVVDQIVSKLIEVVKKKNKAGVSVKPFQVKNHVWVFVNCLIENPTFDSQTKENMTLQPKGFGSKCQLSEKFSKAASNCGIVESILNWVKFKAQTQLNKKCSSVKHSKIKGIPKLDDANDAGGKHSLDCTLILTEGDSAKSLAVSGLGVIGRDRYGVFPLRGKILNVREASHKQIMENAEINNIIKIVGLQYKKSYDDNESLKTLRYGKIMIMTDQDQDGSHIKGLLINFFHHNWPSLLKHSFLEEFITPIVKATKNKQELSFYSIPEFEEWKKQTENQKLWKIKYYKGLGTSTSKEAKEYFADMDRHRIIFRYAGPEDDAAITLAFSKKKVDDRKEWLTNFMEDRRQRRLHGLPEQFLYGTATKHLTYNDFINKELILFSNSDNERSIPSLVDGLKPGQRKVLFTCFKRNDKREVKVAQLAGSVAEMSAYHHGEQALMMTIVNLAQNFVGSNNVSLLQPIGQFGTRLHGGKDAASPRYIFTMLSPLARLLYPSVDDNLLKFLYDDNQRVEPEWYIPIIPLVLVNGAEGIGTGWACKLPNYDTREIVNNVKRMLDGLDPYPLLPSYKNFKGTIQELGQNQYAMSGEIYVLDRNTIEITELPVRTWTQVYKEQVLEPMLNGTEKTPSLISDYKEYHTDTTVKFVVKMTEEKLAQAEAAGLHKVFKLQTSLTCNSMVLFDHMGCLKKYETVQDILKEFFDLRLNYYTLRKEWLIGMLGAEAARLNNQARFVLEKIQGKISIENKTKRDLIQMLVQKGFESDPVKAWKEAQEKATEEDDLQDPNEDNSSDSGTGSGPDFNYILNMSLWSLSKEKVDELVRQRDIKSKELNDLKRKSPPDLWKEDLAAFVEELDRVEAQEKEDVLAGFAGKAIKGKIGKPKMKKLQLEETLPSLYGRRVEPQISAMKADASKKMLKKKKGEIDSIALKMEFDEEFSSAPAEGGAEDSMNTTPVKPVKPKRERKEPGPRAKKTVLTPKSSTKKGKKRNPWSDNESKSDSDLEEAEAEPVVIPRDSLLRRAAAERPKYTFDFSEEEDADDDDEGVISRSNNNNNINDLKPPASPAANSRGEDDDVLSDSQEKDDYEFSPFKSTPAAQKPSETQKKKDYIFSSSSFSEKVEESAKYDSDDDDDDEPSPLYSSYSFKSTEKPASKGSSEKESTKYNSDDEDDDDDDVPFSSFTFKSSEKPASKGSAKKTKSLSDGTPKPKRPPKPKKSETSLISDSDLLDFGASKKSATPKAKGRGRKRKTSDSENEGDYNPGRKPTKPASSSKKSKKASFEQDSDGDLFPSEVGSETASRQRPGRARKEVKYFADSDDEDDFAMF

Target ORF information:

RefSeq Version	XM_018095006.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis DNA topoisomerase II beta (top2b), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_018095006.2

ORF Insert Sequence:

ATGTCTAAAT CTGGCGGCGG CGGCGGCGGA GGAGCTGGAG GAGGGAGCGG AGGGCTCACC 
TGGGTGACAC TTTTTGATAA TCAGAACGCA TCAAAAAAGG AAGAAACAGA AAGCAATAAT 
AAAAATGACT CCTCCAAGAA GATGTCCGTA GAAAGAGTTT ACCAGAAAAA GACCCAGCTT 
GAGCATATTC TTCTGCGACC TGACACATAC ATTGGATCTG TGGAGACAGT TACTCAGTCC 
ATGTGGGTTT ATGATGAAGA TGTTGGAATG AATTGCCGTG ACATAACCTA TGTGCCCGGT 
TTATACAAGA TATTTGATGA AATCCTTGTA AACGCAGCTG ATAATAAACA GAGAGATAAA 
AATATGAACT GCATAAAAAT TTCTATTGAT CCTGATTCTA ACATCATCAG CATTTGGAAT 
AATGGTAAAG GAATACCTGT GGTTGAGCAC AAAGTGGAGA AAATGTATGT CCCTGCTTTA 
ATTTTTGGCC AGTTATTAAC ATCAAGCAAC TATGATGATG AGGAAAAGAA AGTTACAGGA 
GGGCGAAATG GATATGGTGC TAAACTTTGC AATATATTCA GCACAAAATT TACTGTGGAG 
ACAGCTTGTA AGGAATACAA GCATAGCTTT AAGCAGACAT GGACAAACAA CATGTTAAAG 
ACAACTGAAC CCAAAATTAA ACATTTTGAT GGAGATGACT ACACATGCAT AACATTCCAG 
CCAGATTTAG CCAAGTTCCA AATGGAAAAA CTGGACAAGG ATACTGTGGC TCTTCTGACA 
AGACGAGCAT ATGATATTGC GGGTTCCTGC AAAGGAGTCA AAGTCTTGCT CAATGGGAAG 
AAGCTTCCGG TAAATGGCTT CCGCAGTTAC GTAGATCTTT ACGTAAAGGA CAGACTTGAT 
GAAACTGGTG TCGCTCTTAA AGTAATCCAT GAAGTTGCAA ATGAAAGATG GGATGTATGT 
TTAACATTGA GCGAAAAGGG ATTCCAACAA ATCAGTTTTG TGAACAGTAT TGCTACCACA 
AAGGGTGGTA GACATGTAGA TTATGTAGTA GACCAAATTG TGTCAAAACT CATTGAAGTT 
GTGAAGAAGA AGAATAAAGC TGGAGTTTCT GTAAAGCCCT TCCAGGTGAA GAATCACGTT 
TGGGTTTTTG TCAACTGTTT AATAGAAAAC CCAACTTTTG ATTCTCAGAC AAAAGAAAAC 
ATGACACTAC AGCCTAAAGG TTTTGGGTCC AAATGCCAGC TGTCAGAAAA ATTTTCAAAA 
GCGGCATCTA ACTGTGGCAT AGTCGAGAGT ATTCTAAATT GGGTTAAATT CAAGGCCCAA 
ACACAGCTAA ATAAGAAATG CTCTTCTGTG AAACACAGTA AAATCAAAGG CATTCCCAAG 
CTGGATGATG CTAATGATGC AGGCGGAAAA CATTCCCTGG ACTGCACTCT GATATTAACT 
GAGGGAGACT CTGCCAAATC TTTAGCTGTT TCAGGCTTAG GTGTCATTGG CCGAGATCGG 
TACGGTGTAT TCCCGCTCAG GGGAAAAATT CTCAATGTTC GAGAAGCTTC CCACAAACAG 
ATTATGGAAA ATGCTGAGAT AAACAACATC ATTAAAATAG TTGGCTTGCA GTACAAAAAA 
AGCTATGATG ATAATGAATC CCTTAAAACC TTGCGTTATG GAAAAATTAT GATAATGACA 
GATCAGGATC AAGATGGCTC GCATATCAAG GGCTTATTGA TTAATTTTTT CCATCACAAT 
TGGCCATCTC TACTGAAACA TAGTTTCCTT GAAGAATTTA TCACACCCAT TGTTAAGGCT 
ACAAAGAATA AGCAGGAGCT TTCATTCTAC AGTATTCCTG AGTTTGAAGA ATGGAAAAAA 
CAAACAGAAA ACCAAAAATT GTGGAAGATT AAGTATTACA AAGGTTTGGG CACCAGCACT 
TCGAAGGAAG CAAAGGAATA CTTTGCTGAC ATGGACAGGC ACAGAATTAT ATTTAGATAT 
GCAGGCCCTG AAGATGATGC AGCTATCACT CTGGCTTTCA GCAAAAAGAA AGTAGATGAC 
AGGAAAGAAT GGCTCACAAA CTTTATGGAA GACAGGCGAC AACGTCGACT ACACGGATTG 
CCCGAGCAAT TCTTGTACGG CACAGCTACA AAACACTTGA CTTACAATGA CTTCATTAAC 
AAGGAGCTGA TTCTTTTTTC AAACTCTGAT AATGAGCGAT CTATTCCATC TCTTGTTGAT 
GGTTTAAAGC CTGGGCAAAG AAAAGTTCTG TTTACTTGTT TTAAAAGGAA TGATAAACGG 
GAAGTCAAGG TTGCTCAGCT GGCTGGTTCT GTTGCTGAAA TGTCCGCTTA CCACCATGGA 
GAGCAAGCCT TGATGATGAC AATAGTAAAT CTTGCCCAGA ACTTTGTGGG AAGTAACAAT 
GTGAGCTTGC TGCAGCCCAT TGGTCAGTTT GGTACTCGAC TCCATGGTGG AAAGGATGCT 
GCCAGCCCTC GTTATATATT CACAATGTTA AGTCCTCTAG CAAGATTGCT CTATCCATCT 
GTGGATGATA ACCTTTTGAA GTTCCTTTAT GATGATAACC AGCGAGTAGA ACCAGAGTGG 
TACATTCCTA TTATACCCCT GGTTTTAGTT AATGGAGCAG AAGGAATCGG TACTGGATGG 
GCTTGTAAAC TGCCCAATTA TGACACAAGA GAAATTGTCA ATAATGTGAA GAGAATGCTG 
GATGGTCTTG ATCCTTATCC ATTGCTTCCA AGCTACAAGA ATTTTAAAGG TACGATTCAG 
GAACTGGGAC AGAATCAGTA TGCAATGAGT GGTGAAATAT ATGTGCTGGA CCGGAACACA 
ATTGAAATCA CAGAACTTCC TGTCAGGACA TGGACCCAGG TTTACAAAGA ACAAGTGCTG 
GAGCCCATGC TCAATGGAAC CGAGAAAACA CCATCGTTAA TCTCTGATTA CAAAGAGTAT 
CATACAGACA CAACTGTAAA ATTTGTTGTC AAAATGACAG AAGAAAAGTT GGCACAGGCA 
GAAGCTGCTG GCTTGCATAA AGTCTTTAAA CTTCAAACAA GTCTTACTTG CAATTCTATG 
GTATTATTTG ACCACATGGG CTGTCTAAAG AAATATGAAA CCGTGCAGGA CATTCTGAAG 
GAATTTTTTG ATCTCCGGTT AAATTACTAC ACCCTGCGCA AAGAATGGCT GATAGGAATG 
TTGGGAGCTG AAGCTGCAAG ACTGAACAAC CAAGCCCGGT TTGTCCTGGA GAAGATACAA 
GGCAAAATCT CTATAGAGAA CAAAACCAAA AGAGATCTAA TTCAGATGTT GGTGCAGAAA 
GGATTTGAGT CAGACCCTGT GAAAGCTTGG AAGGAGGCAC AAGAAAAAGC AACAGAGGAG 
GATGACCTTC AAGATCCGAA TGAAGACAAT TCATCCGACT CTGGCACTGG ATCAGGACCG 
GATTTTAACT ACATTCTCAA TATGTCTCTC TGGAGCCTTT CAAAGGAAAA AGTAGATGAG 
CTTGTCAGAC AGAGGGATAT AAAGAGCAAA GAACTGAATG ACCTCAAGAG GAAGTCTCCG 
CCTGATCTGT GGAAGGAAGA CTTGGCAGCT TTTGTTGAAG AGCTAGACAG AGTGGAAGCT 
CAAGAAAAAG AAGATGTCTT AGCAGGGTTT GCAGGCAAAG CAATAAAAGG CAAAATTGGA 
AAACCCAAAA TGAAGAAATT ACAGCTTGAG GAGACTTTGC CATCTCTTTA CGGCAGAAGA 
GTAGAGCCAC AAATCAGTGC AATGAAAGCA GATGCCAGCA AAAAGATGCT AAAGAAAAAG 
AAGGGTGAAA TTGACTCTAT TGCACTGAAA ATGGAATTTG ATGAGGAGTT TAGCTCGGCA 
CCAGCAGAGG GAGGAGCTGA AGATTCCATG AATACCACTC CTGTAAAACC TGTAAAGCCC 
AAGAGAGAAA GAAAAGAACC AGGTCCTCGA GCCAAAAAGA CTGTATTAAC ACCCAAGTCA 
TCAACCAAGA AAGGGAAGAA GCGAAACCCT TGGTCAGACA ATGAGTCAAA ATCAGACAGT 
GACCTGGAAG AAGCTGAGGC AGAGCCTGTG GTCATTCCTC GGGACTCTCT CCTAAGGAGA 
GCAGCTGCTG AAAGACCGAA GTACACGTTT GATTTCTCTG AAGAGGAAGA TGCAGACGAT 
GACGACGAAG GTGTCATTAG CCGTAGTAAT AATAATAATA ATATTAATGA CTTAAAACCG 
CCAGCTTCTC CTGCTGCTAA TAGCCGAGGT GAAGATGATG ATGTTCTCTC TGACAGCCAA 
GAAAAGGATG ACTATGAATT CTCCCCTTTC AAGTCTACCC CAGCCGCACA AAAGCCTTCA 
GAAACACAAA AGAAAAAAGA CTACATATTT TCATCATCTT CATTTTCAGA AAAAGTAGAA 
GAATCTGCAA AATATGACAG TGATGATGAT GATGATGATG AACCCTCCCC CCTTTACTCC 
TCATATTCCT TCAAATCTAC AGAAAAGCCA GCCAGCAAAG GATCTAGTGA GAAAGAATCC 
ACCAAATATA ACAGTGACGA TGAGGATGAT GACGATGACG ATGTACCGTT TTCGTCGTTT 
ACCTTTAAAT CTTCCGAAAA GCCAGCCAGT AAAGGTTCTG CTAAGAAAAC AAAATCTTTG 
TCAGATGGTA CTCCTAAACC AAAACGACCG CCAAAACCAA AGAAGTCTGA GACTTCATTG 
ATTTCCGACT CTGACTTGTT AGATTTTGGT GCATCAAAGA AATCTGCAAC ACCCAAAGCA 
AAAGGAAGGG GAAGGAAACG AAAAACATCT GATTCTGAAA ATGAAGGAGA TTACAATCCA 
GGGCGAAAAC CTACAAAACC TGCATCTTCT AGCAAAAAAT CCAAGAAAGC ATCATTTGAG 
CAGGATTCAG ATGGTGATCT CTTCCCTTCA GAAGTCGGCT CTGAGACCGC ATCGAGGCAA 
AGGCCTGGCC GGGCTAGGAA AGAAGTCAAA TATTTTGCAG ACTCTGACGA TGAAGATGAT 
TTTGCTATGT TTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

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top2b cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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