rnf216 cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

Gene
Clones
gRNAs

Summary

Gene Symbol	rnf216
Entrez Gene ID	549588
Full Name	ring finger protein 216
Synonyms	2810055g22rik,triad3,u7i1,ubce7ip1,zin
General protein information	Preferred Names ring finger protein 216 Names E3 ubiquitin-protein ligase RNF216
Gene Type	protein-coding
Organism	Xenopus tropicalis(tropical clawed frog)

mRNA and Protein(s)

mRNA	Protein	Name
XM_012970439.2	XP_012825893.1	E3 ubiquitin-protein ligase RNF216 isoform X2
XM_018097114.2	XP_017952603.1	E3 ubiquitin-protein ligase RNF216 isoform X3
XM_012970438.2	XP_012825892.1	E3 ubiquitin-protein ligase RNF216 isoform X1
XM_012970438.3	XP_012825892.2	E3 ubiquitin-protein ligase RNF216 isoform X1
XM_012970439.3	XP_012825893.2	E3 ubiquitin-protein ligase RNF216 isoform X2
XM_018097114.1	XP_017952603.1	E3 ubiquitin-protein ligase RNF216 isoform X4
NM_001016834.2	NP_001016834.1	E3 ubiquitin-protein ligase RNF216
XM_018097113.1	XP_017952602.1	E3 ubiquitin-protein ligase RNF216 isoform X3

Pathways from BioSystems

Homologies

Mus musculus (house mouse)	Rnf216	NP_996993.1
Xenopus tropicalis (tropical clawed frog)	rnf216	NP_001016834.1
Rattus norvegicus (Norway rat)	Rnf216	NP_001100592.1
Macaca mulatta (Rhesus monkey)	RNF216	XP_002803244.1
Danio rerio (zebrafish)	rnf216	XP_687871.3
Homo sapiens (human)	RNF216	NP_996994.1
Bos taurus (cattle)	RNF216	NP_001180187.1
Pan troglodytes (chimpanzee)	RNF216	XP_518955.2
Canis lupus familiaris (dog)	RNF216	XP_536887.2
Gallus gallus (chicken)	RNF216	XP_414793.4

Gene Ontology
Bibliography

Related articles in PubMed

Genetic and genomic tools for Xenopus research: The NIH Xenopus initiative.
Klein SL, Strausberg RL, Wagner L, Pontius J, Clifton SW, Richardson P
Developmental dynamics : an official publication of the American Association of Anatomists225(4)384-91(2002 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following rnf216 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the rnf216 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OXa20689	NM_001016834.2 Latest version!	Xenopus tropicalis ring finger protein 216 (rnf216), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
OXa27924	XM_012970438.2 Latest version!	Xenopus tropicalis ring finger protein 216 (rnf216), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
OXa27925	XM_012970439.2 Latest version!	Xenopus tropicalis ring finger protein 216 (rnf216), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
OXa46587	XM_018097113.1 Latest version!	Xenopus tropicalis ring finger protein 216 (rnf216), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
OXa46588	XM_018097114.1 Latest version!	Xenopus tropicalis ring finger protein 216 (rnf216), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
OXa66045	XM_012970438.3 Latest version!	Xenopus tropicalis ring finger protein 216 (rnf216), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
OXa66046	XM_012970439.3 Latest version!	Xenopus tropicalis ring finger protein 216 (rnf216), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
OXa66047	XM_018097114.2 Latest version!	Xenopus tropicalis ring finger protein 216 (rnf216), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

rnf216 ( NM_001016834.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> NP_001016834.1 Xenopus tropicalis ring finger protein 216 (rnf216), mRNA.

Price & Availability↑

CloneID	OXa20689
Clone ID Related Accession (Same CDS sequence)	NM_001016834.2
Accession Version	NM_001016834.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2517bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1576944000000
Organism	Xenopus tropicalis(tropical clawed frog)
Product	E3 ubiquitin-protein ligase RNF216
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from CR855491.2. On Oct 14, 2005 this sequence version replaced NM_001016834.1. ##Evidence-Data-START## Transcript exon combination :: CR855491.2 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMD00028290, SAMD00028291 [ECO:0000350] ##Evidence-Data-END##

ATGGAGTCAC AGGATGAGAT GGCTGGAATA AATCCTTTAT GTCATAGAGG AAAAGACTGG 
GACACACACA GGAATGATGC ACCAGTCACT ATATCTGATG ACTCTGATGA AGATCTATCT 
TTTGTCCTTT GCAAGCCAGC ACAGGACTAT GAGGAAGATG AAGATGATGA TGATGATGAT 
GATGTTGTCA TTTTAGAGGA AAGACAACTA AACCACCGTC CTGACCTCAT TCGACCAGCG 
GCTGCGTGGC ACAGTGGGAA CCCATCAGTT AGTGGAAGTC GTCGGCCTTT GTGCAGGAAA 
AGTCTACATG AACAGCATTC TAGAAGTAAT GGGAAGGCTG AATTTAAAGA AGAGATAGAT 
AGAGATATTG TGGATGGAAC CCTTTTGAGC AACAGAGTTC TAGACAGCGA AGAAGATCTC 
TATATAACTC CCACAAAATC TGTTTGCGAT TCTTCGGGTC CTTCTATAAA TAAAGAGACT 
ATTTGTCACA AATCTGTTGG TAAACATGTT GAACCAGTAC CAGGACCAGC TTTTCCCAGG 
ACCCATCTAG TGGAGGATGA AATTCCAGGA CCCTTATCCC CTCCGATGGC CCATCCTTCA 
GATGCACCAG AAAAAGAAAA TCATACAGCA AGTGACCTGC CAAAAAGAGC AACAGGACCA 
ACTGAGTTAA ACCCTGGTAA TCTGCCTGAT CAGGGAGCTG CAGGACTTGA CTATGAGCTG 
ATATCTGACC TCATTAAGGA AACTGAAGCA AGATTTCCAG ATGTTAAACA AGAGTATGTG 
AAAGAACTGA TTCTCTCCAA ACAATGCTAC GATTTAAATG TCATATGTAA CACCCTTCTG 
GAAGATCCTG ACTACCCAAA GAGAGATGGT TTGGCTGTTC TAAATAAGAG TAGCCGTTTA 
CTTACCAGTC ATGCGGATCC CAAGGTGACC ATGGTGGACT ATTTTAATTT TTCACAACTC 
GCTCCCCTAG ATCAACGGTG CCTAATTCAA GCATCAGACC TCCTAATGGC TGATTTTAAA 
ATGCTCAGCA GTCAAGATAT TAAGTGGGCT TTGCATGAAC TTAAAGGACA CTATGCCATA 
ACCAGAAAGG CTTTTTCTGA TGCTATCAAA CAGTGGCGGG AGTTGTCTCC AGACCCAGGT 
GGGAAGCGCA AAAAAAGAAA AGAGATGAAC CAGTTTTCCT ATATTGATTT CATATTTGAG 
CAAGCTCCAC CGCTCCGTCC GTCGTCCCTC TCCCGCTCCC TGCATCGGGG GCCAGGAAAT 
GTAACTATTG AGAAGCGAAT GTACTTTTTG GAAAACAAGA GACGGCATTA CAAAACATAT 
GACAAAAATT CACTACTCCC TGCTGTGCGA GAGGAACTAG AATTCTTTGA ACGAAAGATG 
AAGGAGATGG CAGAGCATGA AGATTTCCTT CTTGCTTTAC AAGTGAATGA GGAACAATAT 
GAGCAGGATG GCCAGATGAT CGAGTGTCGC TGTTGCTACG GTTCATTTGC CTTTGAAGAG 
TTGACTCAAT GTGCAGATGG GCATTTGTTC TGCAAGGAGT GCCTCATAAA ATATGCACAG 
GAGGCTGCCT TTGGCTCTGG GCAGCCCAAA CTGATCTGCA TGGAAGGCAC TTGCCCCTGT 
GAGTTTCCAA CAAGTGAAGT AGAAAAAGTT CTTCCAGAGA ATATTCTTCA AAAATATTAT 
GAAAGGAAAG CTGAAGAAGA CATCTCTGCA GCATGTGCAG ATCAGCTGGT TCGGTGTCCA 
TCTTGCAGTT TCCCTGCACT TCTTGATAAA GATGTGGATA GATTTAGCTG CCCAAACCCT 
CATTGTCGAA AAGAAACATG TAAGAAATGT CAGAAGTTGT GGAAGGAACA TGTAAACCTG 
ACCTGTGAAG AACTGGCAGA AAGTGATGAT GTTAACTACA GAACCTTCAT TGAAGAAAAA 
ATGACTGCAG CCTGTGTTCG GAAATGCCAT ATATGTGCTA CAGAACTCAT CAAAGCAGAA 
GGGTGCAACA GATTATCATG TCGCTGTGGG GCACAAATAT GCTACTTGTG TCGAGCTCCT 
ATCAATGGCT ATGACCACTT CTGTCAGCAC CCACATTCTC CTGGTGCACC ATGCCGGGAG 
TGTACCCGTT GCTCTTTGTG GACTGATCCA TCTCAAGATG ATGAGAGATT AATCCTGGAG 
ATCCAAAGAG ATGCAGAGAA TGAACAAAGG AGCCGAAAAG GCGAGAAAGC TGTCAAAAGA 
ATAGGACCTC CTATTCAGAA GAACCCTTCC AAGTCTTCAA GGATAGAGAC AGTGCCAAGA 
GAACGACCCT TAAATCAGCC GCTGATACCT CAGTTTCCCT TAATGCACCC AGCTTTTCCT 
CTTCCCCCTG TCCAGCCAAT GTATAATAAC TTCCAATTGA ATTTAGGAGC CCAACATGCC 
CCATATGTGC CTCCACTAGC CAACATTCGG TTGAACTATA ATGTTCACCC AATTGACATG 
GATTTAGAGC CGCACTTGCC TATGCATTTT GGACCACAGC CTCGGCGTGA GTTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001016834.1
CDS	181..2697
Translation	MESQDEMAGINPLCHRGKDWDTHRNDAPVTISDDSDEDLSFVLCKPAQDYEEDEDDDDDDDVVILEERQLNHRPDLIRPAAAWHSGNPSVSGSRRPLCRKSLHEQHSRSNGKAEFKEEIDRDIVDGTLLSNRVLDSEEDLYITPTKSVCDSSGPSINKETICHKSVGKHVEPVPGPAFPRTHLVEDEIPGPLSPPMAHPSDAPEKENHTASDLPKRATGPTELNPGNLPDQGAAGLDYELISDLIKETEARFPDVKQEYVKELILSKQCYDLNVICNTLLEDPDYPKRDGLAVLNKSSRLLTSHADPKVTMVDYFNFSQLAPLDQRCLIQASDLLMADFKMLSSQDIKWALHELKGHYAITRKAFSDAIKQWRELSPDPGGKRKKRKEMNQFSYIDFIFEQAPPLRPSSLSRSLHRGPGNVTIEKRMYFLENKRRHYKTYDKNSLLPAVREELEFFERKMKEMAEHEDFLLALQVNEEQYEQDGQMIECRCCYGSFAFEELTQCADGHLFCKECLIKYAQEAAFGSGQPKLICMEGTCPCEFPTSEVEKVLPENILQKYYERKAEEDISAACADQLVRCPSCSFPALLDKDVDRFSCPNPHCRKETCKKCQKLWKEHVNLTCEELAESDDVNYRTFIEEKMTAACVRKCHICATELIKAEGCNRLSCRCGAQICYLCRAPINGYDHFCQHPHSPGAPCRECTRCSLWTDPSQDDERLILEIQRDAENEQRSRKGEKAVKRIGPPIQKNPSKSSRIETVPRERPLNQPLIPQFPLMHPAFPLPPVQPMYNNFQLNLGAQHAPYVPPLANIRLNYNVHPIDMDLEPHLPMHFGPQPRREL

Target ORF information:

RefSeq Version	NM_001016834.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis ring finger protein 216 (rnf216), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001016834.2

ORF Insert Sequence:

ATGGAGTCAC AGGATGAGAT GGCTGGAATA AATCCTTTAT GTCATAGAGG AAAAGACTGG 
GACACACACA GGAATGATGC ACCAGTCACT ATATCTGATG ACTCTGATGA AGATCTATCT 
TTTGTCCTTT GCAAGCCAGC ACAGGACTAT GAGGAAGATG AAGATGATGA TGATGATGAT 
GATGTTGTCA TTTTAGAGGA AAGACAACTA AACCACCGTC CTGACCTCAT TCGACCAGCG 
GCTGCGTGGC ACAGTGGGAA CCCATCAGTT AGTGGAAGTC GTCGGCCTTT GTGCAGGAAA 
AGTCTACATG AACAGCATTC TAGAAGTAAT GGGAAGGCTG AATTTAAAGA AGAGATAGAT 
AGAGATATTG TGGATGGAAC CCTTTTGAGC AACAGAGTTC TAGACAGCGA AGAAGATCTC 
TATATAACTC CCACAAAATC TGTTTGCGAT TCTTCGGGTC CTTCTATAAA TAAAGAGACT 
ATTTGTCACA AATCTGTTGG TAAACATGTT GAACCAGTAC CAGGACCAGC TTTTCCCAGG 
ACCCATCTAG TGGAGGATGA AATTCCAGGA CCCTTATCCC CTCCGATGGC CCATCCTTCA 
GATGCACCAG AAAAAGAAAA TCATACAGCA AGTGACCTGC CAAAAAGAGC AACAGGACCA 
ACTGAGTTAA ACCCTGGTAA TCTGCCTGAT CAGGGAGCTG CAGGACTTGA CTATGAGCTG 
ATATCTGACC TCATTAAGGA AACTGAAGCA AGATTTCCAG ATGTTAAACA AGAGTATGTG 
AAAGAACTGA TTCTCTCCAA ACAATGCTAC GATTTAAATG TCATATGTAA CACCCTTCTG 
GAAGATCCTG ACTACCCAAA GAGAGATGGT TTGGCTGTTC TAAATAAGAG TAGCCGTTTA 
CTTACCAGTC ATGCGGATCC CAAGGTGACC ATGGTGGACT ATTTTAATTT TTCACAACTC 
GCTCCCCTAG ATCAACGGTG CCTAATTCAA GCATCAGACC TCCTAATGGC TGATTTTAAA 
ATGCTCAGCA GTCAAGATAT TAAGTGGGCT TTGCATGAAC TTAAAGGACA CTATGCCATA 
ACCAGAAAGG CTTTTTCTGA TGCTATCAAA CAGTGGCGGG AGTTGTCTCC AGACCCAGGT 
GGGAAGCGCA AAAAAAGAAA AGAGATGAAC CAGTTTTCCT ATATTGATTT CATATTTGAG 
CAAGCTCCAC CGCTCCGTCC GTCGTCCCTC TCCCGCTCCC TGCATCGGGG GCCAGGAAAT 
GTAACTATTG AGAAGCGAAT GTACTTTTTG GAAAACAAGA GACGGCATTA CAAAACATAT 
GACAAAAATT CACTACTCCC TGCTGTGCGA GAGGAACTAG AATTCTTTGA ACGAAAGATG 
AAGGAGATGG CAGAGCATGA AGATTTCCTT CTTGCTTTAC AAGTGAATGA GGAACAATAT 
GAGCAGGATG GCCAGATGAT CGAGTGTCGC TGTTGCTACG GTTCATTTGC CTTTGAAGAG 
TTGACTCAAT GTGCAGATGG GCATTTGTTC TGCAAGGAGT GCCTCATAAA ATATGCACAG 
GAGGCTGCCT TTGGCTCTGG GCAGCCCAAA CTGATCTGCA TGGAAGGCAC TTGCCCCTGT 
GAGTTTCCAA CAAGTGAAGT AGAAAAAGTT CTTCCAGAGA ATATTCTTCA AAAATATTAT 
GAAAGGAAAG CTGAAGAAGA CATCTCTGCA GCATGTGCAG ATCAGCTGGT TCGGTGTCCA 
TCTTGCAGTT TCCCTGCACT TCTTGATAAA GATGTGGATA GATTTAGCTG CCCAAACCCT 
CATTGTCGAA AAGAAACATG TAAGAAATGT CAGAAGTTGT GGAAGGAACA TGTAAACCTG 
ACCTGTGAAG AACTGGCAGA AAGTGATGAT GTTAACTACA GAACCTTCAT TGAAGAAAAA 
ATGACTGCAG CCTGTGTTCG GAAATGCCAT ATATGTGCTA CAGAACTCAT CAAAGCAGAA 
GGGTGCAACA GATTATCATG TCGCTGTGGG GCACAAATAT GCTACTTGTG TCGAGCTCCT 
ATCAATGGCT ATGACCACTT CTGTCAGCAC CCACATTCTC CTGGTGCACC ATGCCGGGAG 
TGTACCCGTT GCTCTTTGTG GACTGATCCA TCTCAAGATG ATGAGAGATT AATCCTGGAG 
ATCCAAAGAG ATGCAGAGAA TGAACAAAGG AGCCGAAAAG GCGAGAAAGC TGTCAAAAGA 
ATAGGACCTC CTATTCAGAA GAACCCTTCC AAGTCTTCAA GGATAGAGAC AGTGCCAAGA 
GAACGACCCT TAAATCAGCC GCTGATACCT CAGTTTCCCT TAATGCACCC AGCTTTTCCT 
CTTCCCCCTG TCCAGCCAAT GTATAATAAC TTCCAATTGA ATTTAGGAGC CCAACATGCC 
CCATATGTGC CTCCACTAGC CAACATTCGG TTGAACTATA ATGTTCACCC AATTGACATG 
GATTTAGAGC CGCACTTGCC TATGCATTTT GGACCACAGC CTCGGCGTGA GTTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

rnf216 ( XM_012970438.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012825892.1 Xenopus tropicalis ring finger protein 216 (rnf216), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OXa27924
Clone ID Related Accession (Same CDS sequence)	XM_012970438.2
Accession Version	XM_012970438.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2661bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1473177600000
Organism	Xenopus tropicalis(tropical clawed frog)
Product	E3 ubiquitin-protein ligase RNF216 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030685.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012970438.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGTCAC AGGATGAGAT GGCTGGAATA AATCCTTTAT GTCATAGAGG AAAAGACTGG 
GACACACACA GGAATGATGC ACCAGTCACT ATATCTGATG ACTCTGATGA AGATCTATCT 
TTTGTCCTTT GCAAGCCAGC ACAGGACTAT GAGGAAGATG AAGATGATGA TGATGATGAT 
GATGTTGTCA TTTTAGAGGA AAGACAACTA AACCACCGTC CTGACCTCAT TCGACCAGCG 
GCTGCGTGGC ACAGTGGGAA CCCATCAGTT AGTGGAAGTC GTCGGCCTTT GTGCAGGAAA 
AGTCTACATG AACAGCATTC TAGAAGTAAT GGGAAGGCTG AATTTAAAGA AGAGATAGAT 
AGAGATATTG TGGATGGAAC CCTTTTGAGC AACAGAGTTC TAGACAGCGA AGAAGATCTC 
TATATAACTC CCACAAAATC TGTTTGCGAT TCTTCGGGTC CTTCTATAAA TAAAGAGACT 
ATTTGTCACA AATCTGTTGG TAAACATGTT GAACCAGTAC CAGGTATTGT GTTGCCATCT 
GTACTTAAGA ACTCTCTTTC CGAACACCCA TTTCATCAGT CTAGTCTCCA GAAAGGATTT 
ACTATAAATT GCCATGCACC AGTGCAAAAA TTAGTAATGA ACAACGATGT TCAATATGAT 
TTCCTAGGAC CAGCTTTTCC CAGGACCCAT CTAGTGGAGG ATGAAATTCC AGGACCCTTA 
TCCCCTCCGA TGGCCCATCC TTCAGATGCA CCAGAAAAAG AAAATCATAC AGCAAGTGAC 
CTGCCAAAAA GAGCAACAGG ACCAACTGAG TTAAACCCTG GTAATCTGCC TGATCAGGGA 
GCTGCAGGAC TTGACTATGA GCTGATATCT GACCTCATTA AGGAAACTGA AGCAAGATTT 
CCAGATGTTA AACAAGAGTA TGTGAAAGAA CTGATTCTCT CCAAACAATG CTACGATTTA 
AATGTCATAT GTAACACCCT TCTGGAAGAT CCTGACTACC CAAAGAGAGA TGGTTTGGCT 
GTTCTAAATA AGAGTAGCCG TTTACTTACC AGTCATGCGG ATCCCAAGGT GACCATGGTG 
GACTATTTTA ATTTTTCACA ACTCGCTCCC CTAGATCAAC GGTGCCTAAT TCAAGCATCA 
GACCTCCTAA TGGCTGATTT TAAAATGCTC AGCAGTCAAG ATATTAAGTG GGCTTTGCAT 
GAACTTAAAG GACACTATGC CATAACCAGA AAGGCTTTTT CTGATGCTAT CAAACAGTGG 
CGGGAGTTGT CTCCAGACCC AGGTGGGAAG CGCAAAAAAA GAAAAGAGAT GAACCAGTTT 
TCCTATATTG ATTTCATATT TGAGCAAGCT CCACCGCTCC GTCCGTCGTC CCTCTCCCGC 
TCCCTGCATC GGGGGCCAGG AAATGTAACT ATTGAGAAGC GAATGTACTT TTTGGAAAAC 
AAGAGACGGC ATTACAAAAC ATATGACAAA AATTCACTAC TCCCTGCTGT GCGAGAGGAA 
CTAGAATTCT TTGAACGAAA GATGAAGGAG ATGGCAGAGC ATGAAGATTT CCTTCTTGCT 
TTACAAGTGA ATGAGGAACA ATATGAGCAG GATGGCCAGA TGATCGAGTG TCGCTGTTGC 
TACGGTTCAT TTGCCTTTGA AGAGTTGACT CAATGTGCAG ATGGGCATTT GTTCTGCAAG 
GAGTGCCTCA TAAAATATGC ACAGGAGGCT GCCTTTGGCT CTGGGCAGCC CAAACTGATC 
TGCATGGAAG GCACTTGCCC CTGTGAGTTT CCAACAAGTG AAGTAGAAAA AGTTCTTCCA 
GAGAATATTC TTCAAAAATA TTATGAAAGG AAAGCTGAAG AAGACATCTC TGCAGCATGT 
GCAGATCAGC TGGTTCGGTG TCCATCTTGC AGTTTCCCTG CACTTCTTGA TAAAGATGTG 
GATAGATTTA GCTGCCCAAA CCCTCATTGT CGAAAAGAAA CATGTAAGAA ATGTCAGAAG 
TTGTGGAAGG AACATGTAAA CCTGACCTGT GAAGAACTGG CAGAAAGTGA TGATGTTAAC 
TACAGAACCT TCATTGAAGA AAAAATGACT GCAGCCTGTG TTCGGAAATG CCATATATGT 
GCTACAGAAC TCATCAAAGC AGAAGGGTGC AACAGATTAT CATGTCGCTG TGGGGCACAA 
ATATGCTACT TGTGTCGAGC TCCTATCAAT GGCTATGACC ACTTCTGTCA GCACCCACAT 
TCTCCTGGTG CACCATGCCG GGAGTGTACC CGTTGCTCTT TGTGGACTGA TCCATCTCAA 
GATGATGAGA GATTAATCCT GGAGATCCAA AGAGATGCAG AGAATGAACA AAGGAGCCGA 
AAAGGCGAGA AAGCTGTCAA AAGAATAGGA CCTCCTATTC AGAAGAACCC TTCCAAGTCT 
TCAAGGATAG AGACAGTGCC AAGAGAACGA CCCTTAAATC AGCCGCTGAT ACCTCAGTTT 
CCCTTAATGC ACCCAGCTTT TCCTCTTCCC CCTGTCCAGC CAATGTATAA TAACTTCCAA 
TTGAATTTAG GAGCCCAACA TGCCCCATAT GTGCCTCCAC TAGCCAACAT TCGGTTGAAC 
TATAATGTTC ACCCAATTGA CATGGATTTA GAGCCGCACT TGCCTATGCA TTTTGGACCA 
CAGCCTCGGC GTGAGTTGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012825892.1
CDS	232..2892
Translation	MESQDEMAGINPLCHRGKDWDTHRNDAPVTISDDSDEDLSFVLCKPAQDYEEDEDDDDDDDVVILEERQLNHRPDLIRPAAAWHSGNPSVSGSRRPLCRKSLHEQHSRSNGKAEFKEEIDRDIVDGTLLSNRVLDSEEDLYITPTKSVCDSSGPSINKETICHKSVGKHVEPVPGIVLPSVLKNSLSEHPFHQSSLQKGFTINCHAPVQKLVMNNDVQYDFLGPAFPRTHLVEDEIPGPLSPPMAHPSDAPEKENHTASDLPKRATGPTELNPGNLPDQGAAGLDYELISDLIKETEARFPDVKQEYVKELILSKQCYDLNVICNTLLEDPDYPKRDGLAVLNKSSRLLTSHADPKVTMVDYFNFSQLAPLDQRCLIQASDLLMADFKMLSSQDIKWALHELKGHYAITRKAFSDAIKQWRELSPDPGGKRKKRKEMNQFSYIDFIFEQAPPLRPSSLSRSLHRGPGNVTIEKRMYFLENKRRHYKTYDKNSLLPAVREELEFFERKMKEMAEHEDFLLALQVNEEQYEQDGQMIECRCCYGSFAFEELTQCADGHLFCKECLIKYAQEAAFGSGQPKLICMEGTCPCEFPTSEVEKVLPENILQKYYERKAEEDISAACADQLVRCPSCSFPALLDKDVDRFSCPNPHCRKETCKKCQKLWKEHVNLTCEELAESDDVNYRTFIEEKMTAACVRKCHICATELIKAEGCNRLSCRCGAQICYLCRAPINGYDHFCQHPHSPGAPCRECTRCSLWTDPSQDDERLILEIQRDAENEQRSRKGEKAVKRIGPPIQKNPSKSSRIETVPRERPLNQPLIPQFPLMHPAFPLPPVQPMYNNFQLNLGAQHAPYVPPLANIRLNYNVHPIDMDLEPHLPMHFGPQPRREL

Target ORF information:

RefSeq Version	XM_012970438.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis ring finger protein 216 (rnf216), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012970438.2

ORF Insert Sequence:

ATGGAGTCAC AGGATGAGAT GGCTGGAATA AATCCTTTAT GTCATAGAGG AAAAGACTGG 
GACACACACA GGAATGATGC ACCAGTCACT ATATCTGATG ACTCTGATGA AGATCTATCT 
TTTGTCCTTT GCAAGCCAGC ACAGGACTAT GAGGAAGATG AAGATGATGA TGATGATGAT 
GATGTTGTCA TTTTAGAGGA AAGACAACTA AACCACCGTC CTGACCTCAT TCGACCAGCG 
GCTGCGTGGC ACAGTGGGAA CCCATCAGTT AGTGGAAGTC GTCGGCCTTT GTGCAGGAAA 
AGTCTACATG AACAGCATTC TAGAAGTAAT GGGAAGGCTG AATTTAAAGA AGAGATAGAT 
AGAGATATTG TGGATGGAAC CCTTTTGAGC AACAGAGTTC TAGACAGCGA AGAAGATCTC 
TATATAACTC CCACAAAATC TGTTTGCGAT TCTTCGGGTC CTTCTATAAA TAAAGAGACT 
ATTTGTCACA AATCTGTTGG TAAACATGTT GAACCAGTAC CAGGTATTGT GTTGCCATCT 
GTACTTAAGA ACTCTCTTTC CGAACACCCA TTTCATCAGT CTAGTCTCCA GAAAGGATTT 
ACTATAAATT GCCATGCACC AGTGCAAAAA TTAGTAATGA ACAACGATGT TCAATATGAT 
TTCCTAGGAC CAGCTTTTCC CAGGACCCAT CTAGTGGAGG ATGAAATTCC AGGACCCTTA 
TCCCCTCCGA TGGCCCATCC TTCAGATGCA CCAGAAAAAG AAAATCATAC AGCAAGTGAC 
CTGCCAAAAA GAGCAACAGG ACCAACTGAG TTAAACCCTG GTAATCTGCC TGATCAGGGA 
GCTGCAGGAC TTGACTATGA GCTGATATCT GACCTCATTA AGGAAACTGA AGCAAGATTT 
CCAGATGTTA AACAAGAGTA TGTGAAAGAA CTGATTCTCT CCAAACAATG CTACGATTTA 
AATGTCATAT GTAACACCCT TCTGGAAGAT CCTGACTACC CAAAGAGAGA TGGTTTGGCT 
GTTCTAAATA AGAGTAGCCG TTTACTTACC AGTCATGCGG ATCCCAAGGT GACCATGGTG 
GACTATTTTA ATTTTTCACA ACTCGCTCCC CTAGATCAAC GGTGCCTAAT TCAAGCATCA 
GACCTCCTAA TGGCTGATTT TAAAATGCTC AGCAGTCAAG ATATTAAGTG GGCTTTGCAT 
GAACTTAAAG GACACTATGC CATAACCAGA AAGGCTTTTT CTGATGCTAT CAAACAGTGG 
CGGGAGTTGT CTCCAGACCC AGGTGGGAAG CGCAAAAAAA GAAAAGAGAT GAACCAGTTT 
TCCTATATTG ATTTCATATT TGAGCAAGCT CCACCGCTCC GTCCGTCGTC CCTCTCCCGC 
TCCCTGCATC GGGGGCCAGG AAATGTAACT ATTGAGAAGC GAATGTACTT TTTGGAAAAC 
AAGAGACGGC ATTACAAAAC ATATGACAAA AATTCACTAC TCCCTGCTGT GCGAGAGGAA 
CTAGAATTCT TTGAACGAAA GATGAAGGAG ATGGCAGAGC ATGAAGATTT CCTTCTTGCT 
TTACAAGTGA ATGAGGAACA ATATGAGCAG GATGGCCAGA TGATCGAGTG TCGCTGTTGC 
TACGGTTCAT TTGCCTTTGA AGAGTTGACT CAATGTGCAG ATGGGCATTT GTTCTGCAAG 
GAGTGCCTCA TAAAATATGC ACAGGAGGCT GCCTTTGGCT CTGGGCAGCC CAAACTGATC 
TGCATGGAAG GCACTTGCCC CTGTGAGTTT CCAACAAGTG AAGTAGAAAA AGTTCTTCCA 
GAGAATATTC TTCAAAAATA TTATGAAAGG AAAGCTGAAG AAGACATCTC TGCAGCATGT 
GCAGATCAGC TGGTTCGGTG TCCATCTTGC AGTTTCCCTG CACTTCTTGA TAAAGATGTG 
GATAGATTTA GCTGCCCAAA CCCTCATTGT CGAAAAGAAA CATGTAAGAA ATGTCAGAAG 
TTGTGGAAGG AACATGTAAA CCTGACCTGT GAAGAACTGG CAGAAAGTGA TGATGTTAAC 
TACAGAACCT TCATTGAAGA AAAAATGACT GCAGCCTGTG TTCGGAAATG CCATATATGT 
GCTACAGAAC TCATCAAAGC AGAAGGGTGC AACAGATTAT CATGTCGCTG TGGGGCACAA 
ATATGCTACT TGTGTCGAGC TCCTATCAAT GGCTATGACC ACTTCTGTCA GCACCCACAT 
TCTCCTGGTG CACCATGCCG GGAGTGTACC CGTTGCTCTT TGTGGACTGA TCCATCTCAA 
GATGATGAGA GATTAATCCT GGAGATCCAA AGAGATGCAG AGAATGAACA AAGGAGCCGA 
AAAGGCGAGA AAGCTGTCAA AAGAATAGGA CCTCCTATTC AGAAGAACCC TTCCAAGTCT 
TCAAGGATAG AGACAGTGCC AAGAGAACGA CCCTTAAATC AGCCGCTGAT ACCTCAGTTT 
CCCTTAATGC ACCCAGCTTT TCCTCTTCCC CCTGTCCAGC CAATGTATAA TAACTTCCAA 
TTGAATTTAG GAGCCCAACA TGCCCCATAT GTGCCTCCAC TAGCCAACAT TCGGTTGAAC 
TATAATGTTC ACCCAATTGA CATGGATTTA GAGCCGCACT TGCCTATGCA TTTTGGACCA 
CAGCCTCGGC GTGAGTTGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

rnf216 ( XM_012970439.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012825893.1 Xenopus tropicalis ring finger protein 216 (rnf216), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OXa27925
Clone ID Related Accession (Same CDS sequence)	XM_012970439.2
Accession Version	XM_012970439.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2610bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1473177600000
Organism	Xenopus tropicalis(tropical clawed frog)
Product	E3 ubiquitin-protein ligase RNF216 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030685.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012970439.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGTCAC AGGATGAGAT GGCTGGAATA AATCCTTTAT GTCATAGAGG AAAAGACTGG 
GACACACACA GGAATGATGC ACCAGTCACT ATATCTGATG ACTCTGATGA AGATCTATCT 
TTTGTCCTTT GCAAGCCAGC ACAGGACTAT GAGGAAGATG AAGATGATGA TGATGATGAT 
GATGTTGTCA TTTTAGAGGA AAGACAACTA AACCACCGTC CTGACCTCAT TCGACCAGCG 
GCTGCGTGGC ACAGTGGGAA CCCATCAGTT AGTGGAAGTC GTCGGCCTTT GTGCAGGAAA 
AGTCTACATG AACAGCATTC TAGAAGTAAT GGGAAGGCTG AATTTAAAGA AGAGATAGAT 
AGAGATATTG TGGATGGAAC CCTTTTGAGC AACAGAGTTC TAGACAGCGA AGAAGATCTC 
TATATAACTC CCACAAAATC TGTTTGCGAT TCTTCGGGTC CTTCTATAAA TAAAGAGACT 
ATTTGTCACA AATCTGTTGG TAAACATGTT GAACCAGTAC CAGGTATTGT GTTGCCATCT 
GTACTTAAGA ACTCTCTTTC CGAACACCCA TTTCATCAGT CTAGTCTCCA GAAAGGATTT 
ACTATAAATT GCCATGCACC AGTGCAAAAA TTAGTAATGA ACAACGATGT TCAATATGAT 
TTCCTAGGAC CAGCTTTTCC CAGGACCCAT CTAGTGGAGG ATGAAATTCC AGGACCCTTA 
TCCCCTCCGA TGGCCCATCC TTCAGATGCA CCAGAAAAAG AAAATCATAC AGCAAGTGAC 
CTGCCAAAAA GAGCAACAGG ACCAACTGAG TTAAACCCTG GTAATCTGCC TGATCAGGGA 
GCTGCAGGAC TTGACTATGA GCTGATATCT GACCTCATTA AGGAAACTGA AGCAAGATTT 
CCAGATGTTA AACAAGAGTA TGTGAAAGAA CTGATTCTCT CCAAACAATG CTACGATTTA 
AATGTCATAT GTAACACCCT TCTGGAAGAT CCTGACTACC CAAAGAGAGA TGGTTTGGCT 
GTTCTAAATA AGAGTAGCCG TTTACTTACC AGTCATGCGG ATCCCAAGGT GACCATGGTG 
GACTATTTTA ATTTTTCACA ACTCGCTCCC CTAGATCAAC GGTGCCTAAT TCAAGCATCA 
GACCTCCTAA TGGCTGATTT TAAAATGCTC AGCAGTCAAG ATATTAAGTG GGCTTTGCAT 
GAACTTAAAG GACACTATGC CATAACCAGA AAGGCTTTTT CTGATGCTAT CAAACAGTGG 
CGGGAGTTGT CTCCAGACCC AGGTGGGAAG CGCAAAAAAA GAAAAGAGAT GAACCAGTTT 
TCCTATATTG ATTTCATATT TGAGCAAGGA AATGTAACTA TTGAGAAGCG AATGTACTTT 
TTGGAAAACA AGAGACGGCA TTACAAAACA TATGACAAAA ATTCACTACT CCCTGCTGTG 
CGAGAGGAAC TAGAATTCTT TGAACGAAAG ATGAAGGAGA TGGCAGAGCA TGAAGATTTC 
CTTCTTGCTT TACAAGTGAA TGAGGAACAA TATGAGCAGG ATGGCCAGAT GATCGAGTGT 
CGCTGTTGCT ACGGTTCATT TGCCTTTGAA GAGTTGACTC AATGTGCAGA TGGGCATTTG 
TTCTGCAAGG AGTGCCTCAT AAAATATGCA CAGGAGGCTG CCTTTGGCTC TGGGCAGCCC 
AAACTGATCT GCATGGAAGG CACTTGCCCC TGTGAGTTTC CAACAAGTGA AGTAGAAAAA 
GTTCTTCCAG AGAATATTCT TCAAAAATAT TATGAAAGGA AAGCTGAAGA AGACATCTCT 
GCAGCATGTG CAGATCAGCT GGTTCGGTGT CCATCTTGCA GTTTCCCTGC ACTTCTTGAT 
AAAGATGTGG ATAGATTTAG CTGCCCAAAC CCTCATTGTC GAAAAGAAAC ATGTAAGAAA 
TGTCAGAAGT TGTGGAAGGA ACATGTAAAC CTGACCTGTG AAGAACTGGC AGAAAGTGAT 
GATGTTAACT ACAGAACCTT CATTGAAGAA AAAATGACTG CAGCCTGTGT TCGGAAATGC 
CATATATGTG CTACAGAACT CATCAAAGCA GAAGGGTGCA ACAGATTATC ATGTCGCTGT 
GGGGCACAAA TATGCTACTT GTGTCGAGCT CCTATCAATG GCTATGACCA CTTCTGTCAG 
CACCCACATT CTCCTGGTGC ACCATGCCGG GAGTGTACCC GTTGCTCTTT GTGGACTGAT 
CCATCTCAAG ATGATGAGAG ATTAATCCTG GAGATCCAAA GAGATGCAGA GAATGAACAA 
AGGAGCCGAA AAGGCGAGAA AGCTGTCAAA AGAATAGGAC CTCCTATTCA GAAGAACCCT 
TCCAAGTCTT CAAGGATAGA GACAGTGCCA AGAGAACGAC CCTTAAATCA GCCGCTGATA 
CCTCAGTTTC CCTTAATGCA CCCAGCTTTT CCTCTTCCCC CTGTCCAGCC AATGTATAAT 
AACTTCCAAT TGAATTTAGG AGCCCAACAT GCCCCATATG TGCCTCCACT AGCCAACATT 
CGGTTGAACT ATAATGTTCA CCCAATTGAC ATGGATTTAG AGCCGCACTT GCCTATGCAT 
TTTGGACCAC AGCCTCGGCG TGAGTTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012825893.1
CDS	230..2839
Translation	MESQDEMAGINPLCHRGKDWDTHRNDAPVTISDDSDEDLSFVLCKPAQDYEEDEDDDDDDDVVILEERQLNHRPDLIRPAAAWHSGNPSVSGSRRPLCRKSLHEQHSRSNGKAEFKEEIDRDIVDGTLLSNRVLDSEEDLYITPTKSVCDSSGPSINKETICHKSVGKHVEPVPGIVLPSVLKNSLSEHPFHQSSLQKGFTINCHAPVQKLVMNNDVQYDFLGPAFPRTHLVEDEIPGPLSPPMAHPSDAPEKENHTASDLPKRATGPTELNPGNLPDQGAAGLDYELISDLIKETEARFPDVKQEYVKELILSKQCYDLNVICNTLLEDPDYPKRDGLAVLNKSSRLLTSHADPKVTMVDYFNFSQLAPLDQRCLIQASDLLMADFKMLSSQDIKWALHELKGHYAITRKAFSDAIKQWRELSPDPGGKRKKRKEMNQFSYIDFIFEQGNVTIEKRMYFLENKRRHYKTYDKNSLLPAVREELEFFERKMKEMAEHEDFLLALQVNEEQYEQDGQMIECRCCYGSFAFEELTQCADGHLFCKECLIKYAQEAAFGSGQPKLICMEGTCPCEFPTSEVEKVLPENILQKYYERKAEEDISAACADQLVRCPSCSFPALLDKDVDRFSCPNPHCRKETCKKCQKLWKEHVNLTCEELAESDDVNYRTFIEEKMTAACVRKCHICATELIKAEGCNRLSCRCGAQICYLCRAPINGYDHFCQHPHSPGAPCRECTRCSLWTDPSQDDERLILEIQRDAENEQRSRKGEKAVKRIGPPIQKNPSKSSRIETVPRERPLNQPLIPQFPLMHPAFPLPPVQPMYNNFQLNLGAQHAPYVPPLANIRLNYNVHPIDMDLEPHLPMHFGPQPRREL

Target ORF information:

RefSeq Version	XM_012970439.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis ring finger protein 216 (rnf216), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012970439.2

ORF Insert Sequence:

ATGGAGTCAC AGGATGAGAT GGCTGGAATA AATCCTTTAT GTCATAGAGG AAAAGACTGG 
GACACACACA GGAATGATGC ACCAGTCACT ATATCTGATG ACTCTGATGA AGATCTATCT 
TTTGTCCTTT GCAAGCCAGC ACAGGACTAT GAGGAAGATG AAGATGATGA TGATGATGAT 
GATGTTGTCA TTTTAGAGGA AAGACAACTA AACCACCGTC CTGACCTCAT TCGACCAGCG 
GCTGCGTGGC ACAGTGGGAA CCCATCAGTT AGTGGAAGTC GTCGGCCTTT GTGCAGGAAA 
AGTCTACATG AACAGCATTC TAGAAGTAAT GGGAAGGCTG AATTTAAAGA AGAGATAGAT 
AGAGATATTG TGGATGGAAC CCTTTTGAGC AACAGAGTTC TAGACAGCGA AGAAGATCTC 
TATATAACTC CCACAAAATC TGTTTGCGAT TCTTCGGGTC CTTCTATAAA TAAAGAGACT 
ATTTGTCACA AATCTGTTGG TAAACATGTT GAACCAGTAC CAGGTATTGT GTTGCCATCT 
GTACTTAAGA ACTCTCTTTC CGAACACCCA TTTCATCAGT CTAGTCTCCA GAAAGGATTT 
ACTATAAATT GCCATGCACC AGTGCAAAAA TTAGTAATGA ACAACGATGT TCAATATGAT 
TTCCTAGGAC CAGCTTTTCC CAGGACCCAT CTAGTGGAGG ATGAAATTCC AGGACCCTTA 
TCCCCTCCGA TGGCCCATCC TTCAGATGCA CCAGAAAAAG AAAATCATAC AGCAAGTGAC 
CTGCCAAAAA GAGCAACAGG ACCAACTGAG TTAAACCCTG GTAATCTGCC TGATCAGGGA 
GCTGCAGGAC TTGACTATGA GCTGATATCT GACCTCATTA AGGAAACTGA AGCAAGATTT 
CCAGATGTTA AACAAGAGTA TGTGAAAGAA CTGATTCTCT CCAAACAATG CTACGATTTA 
AATGTCATAT GTAACACCCT TCTGGAAGAT CCTGACTACC CAAAGAGAGA TGGTTTGGCT 
GTTCTAAATA AGAGTAGCCG TTTACTTACC AGTCATGCGG ATCCCAAGGT GACCATGGTG 
GACTATTTTA ATTTTTCACA ACTCGCTCCC CTAGATCAAC GGTGCCTAAT TCAAGCATCA 
GACCTCCTAA TGGCTGATTT TAAAATGCTC AGCAGTCAAG ATATTAAGTG GGCTTTGCAT 
GAACTTAAAG GACACTATGC CATAACCAGA AAGGCTTTTT CTGATGCTAT CAAACAGTGG 
CGGGAGTTGT CTCCAGACCC AGGTGGGAAG CGCAAAAAAA GAAAAGAGAT GAACCAGTTT 
TCCTATATTG ATTTCATATT TGAGCAAGGA AATGTAACTA TTGAGAAGCG AATGTACTTT 
TTGGAAAACA AGAGACGGCA TTACAAAACA TATGACAAAA ATTCACTACT CCCTGCTGTG 
CGAGAGGAAC TAGAATTCTT TGAACGAAAG ATGAAGGAGA TGGCAGAGCA TGAAGATTTC 
CTTCTTGCTT TACAAGTGAA TGAGGAACAA TATGAGCAGG ATGGCCAGAT GATCGAGTGT 
CGCTGTTGCT ACGGTTCATT TGCCTTTGAA GAGTTGACTC AATGTGCAGA TGGGCATTTG 
TTCTGCAAGG AGTGCCTCAT AAAATATGCA CAGGAGGCTG CCTTTGGCTC TGGGCAGCCC 
AAACTGATCT GCATGGAAGG CACTTGCCCC TGTGAGTTTC CAACAAGTGA AGTAGAAAAA 
GTTCTTCCAG AGAATATTCT TCAAAAATAT TATGAAAGGA AAGCTGAAGA AGACATCTCT 
GCAGCATGTG CAGATCAGCT GGTTCGGTGT CCATCTTGCA GTTTCCCTGC ACTTCTTGAT 
AAAGATGTGG ATAGATTTAG CTGCCCAAAC CCTCATTGTC GAAAAGAAAC ATGTAAGAAA 
TGTCAGAAGT TGTGGAAGGA ACATGTAAAC CTGACCTGTG AAGAACTGGC AGAAAGTGAT 
GATGTTAACT ACAGAACCTT CATTGAAGAA AAAATGACTG CAGCCTGTGT TCGGAAATGC 
CATATATGTG CTACAGAACT CATCAAAGCA GAAGGGTGCA ACAGATTATC ATGTCGCTGT 
GGGGCACAAA TATGCTACTT GTGTCGAGCT CCTATCAATG GCTATGACCA CTTCTGTCAG 
CACCCACATT CTCCTGGTGC ACCATGCCGG GAGTGTACCC GTTGCTCTTT GTGGACTGAT 
CCATCTCAAG ATGATGAGAG ATTAATCCTG GAGATCCAAA GAGATGCAGA GAATGAACAA 
AGGAGCCGAA AAGGCGAGAA AGCTGTCAAA AGAATAGGAC CTCCTATTCA GAAGAACCCT 
TCCAAGTCTT CAAGGATAGA GACAGTGCCA AGAGAACGAC CCTTAAATCA GCCGCTGATA 
CCTCAGTTTC CCTTAATGCA CCCAGCTTTT CCTCTTCCCC CTGTCCAGCC AATGTATAAT 
AACTTCCAAT TGAATTTAGG AGCCCAACAT GCCCCATATG TGCCTCCACT AGCCAACATT 
CGGTTGAACT ATAATGTTCA CCCAATTGAC ATGGATTTAG AGCCGCACTT GCCTATGCAT 
TTTGGACCAC AGCCTCGGCG TGAGTTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

rnf216 ( XM_018097113.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_017952602.1 Xenopus tropicalis ring finger protein 216 (rnf216), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OXa46587
Clone ID Related Accession (Same CDS sequence)	XM_018097113.1
Accession Version	XM_018097113.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2355bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1473177600000
Organism	Xenopus tropicalis(tropical clawed frog)
Product	E3 ubiquitin-protein ligase RNF216 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030685.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGTCAC AGGATGAGAT GGCTGGAATA AATCCTTTAT GTCATAGAGG AAAAGACTGG 
GACACACACA GGAATGATGC ACCAGTCACT ATATCTGATG ACTCTGATGA AGATCTATCT 
TTTGTCCTTT GCAAGCCAGC ACAGGACTAT GAGGAAGATG AAGATGATGA TGATGATGAT 
GATGTTGTCA TTTTAGAGGA AAGACAACTA AACCACCGTC CTGACCTCAT TCGACCAGCG 
GCTGCGTGGC ACAGTGGGAA CCCATCAGTT AGTGGAAGTC GTCGGCCTTT GTGCAGGAAA 
AGTCTACATG AACAGCATTC TAGAAGTAAT GGGAAGGCTG AATTTAAAGA AGAGATAGAT 
AGAGATATTG TGGATGGAAC CCTTTTGAGC AACAGAGTTC TAGACAGCGA AGAAGATCTC 
TATATAACTC CCACAAAATC TGTTTGCGAT TCTTCGGGTC CTTCTATAAA TAAAGAGACT 
ATTTGTCACA AATCTGTTGG TAAACATGTT GAACCAGTAC CAGGTATTGT GTTGCCATCT 
GTACTTAAGA ACTCTCTTTC CGAACACCCA TTTCATCAGT CTAGTCTCCA GAAAGGATTT 
ACTATAAATT GCCATGCACC AGTGCAAAAA TTAGTAATGA ACAACGATGT TCAATATGAT 
TTCCTAGGAC CAGCTTTTCC CAGGACCCAT CTAGTGGAGG ATGAAATTCC AGGACCCTTA 
TCCCCTCCGA TGGCCCATCC TTCAGATGCA CCAGAAAAAG AAAATCATAC AGCAAGTGAC 
CTGCCAAAAA GAGCAACAGG ACCAACTGAG TTAAACCCTG GTAATCTGCC TGATCAGGGA 
GCTGCAGGAC TTGACTATGA GCTGATATCT GACCTCATTA AGGAAACTGA AGCAAGATTT 
CCAGATGTTA AACAAGAGTA TGTGAAAGAA CTGATTCTCT CCAAACAATG CTACGATTTA 
AATGTCATAT GTAACACCCT TCTGGAAGAT CCTGACTACC CAAAGAGAGA TGGTTTGGCT 
GTTCTAAATA AGAGTAGCCG TTTACTTACC AGTCATGCGG ATCCCAAGGT GACCATGGTG 
GACTATTTTA ATTTTTCACA ACTCGCTCCC CTAGATCAAC GGTGCCTAAT TCAAGCATCA 
GACCTCCTAA TGGCTGATTT TAAAATGCTC AGCAGTCAAG ATATTAAGTG GGCTTTGCAT 
GAACTTAAAG GACACTATGC CATAACCAGA AAGCATGAAG ATTTCCTTCT TGCTTTACAA 
GTGAATGAGG AACAATATGA GCAGGATGGC CAGATGATCG AGTGTCGCTG TTGCTACGGT 
TCATTTGCCT TTGAAGAGTT GACTCAATGT GCAGATGGGC ATTTGTTCTG CAAGGAGTGC 
CTCATAAAAT ATGCACAGGA GGCTGCCTTT GGCTCTGGGC AGCCCAAACT GATCTGCATG 
GAAGGCACTT GCCCCTGTGA GTTTCCAACA AGTGAAGTAG AAAAAGTTCT TCCAGAGAAT 
ATTCTTCAAA AATATTATGA AAGGAAAGCT GAAGAAGACA TCTCTGCAGC ATGTGCAGAT 
CAGCTGGTTC GGTGTCCATC TTGCAGTTTC CCTGCACTTC TTGATAAAGA TGTGGATAGA 
TTTAGCTGCC CAAACCCTCA TTGTCGAAAA GAAACATGTA AGAAATGTCA GAAGTTGTGG 
AAGGAACATG TAAACCTGAC CTGTGAAGAA CTGGCAGAAA GTGATGATGT TAACTACAGA 
ACCTTCATTG AAGAAAAAAT GACTGCAGCC TGTGTTCGGA AATGCCATAT ATGTGCTACA 
GAACTCATCA AAGCAGAAGG GTGCAACAGA TTATCATGTC GCTGTGGGGC ACAAATATGC 
TACTTGTGTC GAGCTCCTAT CAATGGCTAT GACCACTTCT GTCAGCACCC ACATTCTCCT 
GGTGCACCAT GCCGGGAGTG TACCCGTTGC TCTTTGTGGA CTGATCCATC TCAAGATGAT 
GAGAGATTAA TCCTGGAGAT CCAAAGAGAT GCAGAGAATG AACAAAGGAG CCGAAAAGGC 
GAGAAAGCTG TCAAAAGAAT AGGACCTCCT ATTCAGAAGA ACCCTTCCAA GTCTTCAAGG 
ATAGAGACAG TGCCAAGAGA ACGACCCTTA AATCAGCCGC TGATACCTCA GTTTCCCTTA 
ATGCACCCAG CTTTTCCTCT TCCCCCTGTC CAGCCAATGT ATAATAACTT CCAATTGAAT 
TTAGGAGCCC AACATGCCCC ATATGTGCCT CCACTAGCCA ACATTCGGTT GAACTATAAT 
GTTCACCCAA TTGACATGGA TTTAGAGCCG CACTTGCCTA TGCATTTTGG ACCACAGCCT 
CGGCGTGAGT TGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017952602.1
CDS	231..2585
Translation	MESQDEMAGINPLCHRGKDWDTHRNDAPVTISDDSDEDLSFVLCKPAQDYEEDEDDDDDDDVVILEERQLNHRPDLIRPAAAWHSGNPSVSGSRRPLCRKSLHEQHSRSNGKAEFKEEIDRDIVDGTLLSNRVLDSEEDLYITPTKSVCDSSGPSINKETICHKSVGKHVEPVPGIVLPSVLKNSLSEHPFHQSSLQKGFTINCHAPVQKLVMNNDVQYDFLGPAFPRTHLVEDEIPGPLSPPMAHPSDAPEKENHTASDLPKRATGPTELNPGNLPDQGAAGLDYELISDLIKETEARFPDVKQEYVKELILSKQCYDLNVICNTLLEDPDYPKRDGLAVLNKSSRLLTSHADPKVTMVDYFNFSQLAPLDQRCLIQASDLLMADFKMLSSQDIKWALHELKGHYAITRKHEDFLLALQVNEEQYEQDGQMIECRCCYGSFAFEELTQCADGHLFCKECLIKYAQEAAFGSGQPKLICMEGTCPCEFPTSEVEKVLPENILQKYYERKAEEDISAACADQLVRCPSCSFPALLDKDVDRFSCPNPHCRKETCKKCQKLWKEHVNLTCEELAESDDVNYRTFIEEKMTAACVRKCHICATELIKAEGCNRLSCRCGAQICYLCRAPINGYDHFCQHPHSPGAPCRECTRCSLWTDPSQDDERLILEIQRDAENEQRSRKGEKAVKRIGPPIQKNPSKSSRIETVPRERPLNQPLIPQFPLMHPAFPLPPVQPMYNNFQLNLGAQHAPYVPPLANIRLNYNVHPIDMDLEPHLPMHFGPQPRREL

Target ORF information:

RefSeq Version	XM_018097113.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis ring finger protein 216 (rnf216), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_018097113.1

ORF Insert Sequence:

ATGGAGTCAC AGGATGAGAT GGCTGGAATA AATCCTTTAT GTCATAGAGG AAAAGACTGG 
GACACACACA GGAATGATGC ACCAGTCACT ATATCTGATG ACTCTGATGA AGATCTATCT 
TTTGTCCTTT GCAAGCCAGC ACAGGACTAT GAGGAAGATG AAGATGATGA TGATGATGAT 
GATGTTGTCA TTTTAGAGGA AAGACAACTA AACCACCGTC CTGACCTCAT TCGACCAGCG 
GCTGCGTGGC ACAGTGGGAA CCCATCAGTT AGTGGAAGTC GTCGGCCTTT GTGCAGGAAA 
AGTCTACATG AACAGCATTC TAGAAGTAAT GGGAAGGCTG AATTTAAAGA AGAGATAGAT 
AGAGATATTG TGGATGGAAC CCTTTTGAGC AACAGAGTTC TAGACAGCGA AGAAGATCTC 
TATATAACTC CCACAAAATC TGTTTGCGAT TCTTCGGGTC CTTCTATAAA TAAAGAGACT 
ATTTGTCACA AATCTGTTGG TAAACATGTT GAACCAGTAC CAGGTATTGT GTTGCCATCT 
GTACTTAAGA ACTCTCTTTC CGAACACCCA TTTCATCAGT CTAGTCTCCA GAAAGGATTT 
ACTATAAATT GCCATGCACC AGTGCAAAAA TTAGTAATGA ACAACGATGT TCAATATGAT 
TTCCTAGGAC CAGCTTTTCC CAGGACCCAT CTAGTGGAGG ATGAAATTCC AGGACCCTTA 
TCCCCTCCGA TGGCCCATCC TTCAGATGCA CCAGAAAAAG AAAATCATAC AGCAAGTGAC 
CTGCCAAAAA GAGCAACAGG ACCAACTGAG TTAAACCCTG GTAATCTGCC TGATCAGGGA 
GCTGCAGGAC TTGACTATGA GCTGATATCT GACCTCATTA AGGAAACTGA AGCAAGATTT 
CCAGATGTTA AACAAGAGTA TGTGAAAGAA CTGATTCTCT CCAAACAATG CTACGATTTA 
AATGTCATAT GTAACACCCT TCTGGAAGAT CCTGACTACC CAAAGAGAGA TGGTTTGGCT 
GTTCTAAATA AGAGTAGCCG TTTACTTACC AGTCATGCGG ATCCCAAGGT GACCATGGTG 
GACTATTTTA ATTTTTCACA ACTCGCTCCC CTAGATCAAC GGTGCCTAAT TCAAGCATCA 
GACCTCCTAA TGGCTGATTT TAAAATGCTC AGCAGTCAAG ATATTAAGTG GGCTTTGCAT 
GAACTTAAAG GACACTATGC CATAACCAGA AAGCATGAAG ATTTCCTTCT TGCTTTACAA 
GTGAATGAGG AACAATATGA GCAGGATGGC CAGATGATCG AGTGTCGCTG TTGCTACGGT 
TCATTTGCCT TTGAAGAGTT GACTCAATGT GCAGATGGGC ATTTGTTCTG CAAGGAGTGC 
CTCATAAAAT ATGCACAGGA GGCTGCCTTT GGCTCTGGGC AGCCCAAACT GATCTGCATG 
GAAGGCACTT GCCCCTGTGA GTTTCCAACA AGTGAAGTAG AAAAAGTTCT TCCAGAGAAT 
ATTCTTCAAA AATATTATGA AAGGAAAGCT GAAGAAGACA TCTCTGCAGC ATGTGCAGAT 
CAGCTGGTTC GGTGTCCATC TTGCAGTTTC CCTGCACTTC TTGATAAAGA TGTGGATAGA 
TTTAGCTGCC CAAACCCTCA TTGTCGAAAA GAAACATGTA AGAAATGTCA GAAGTTGTGG 
AAGGAACATG TAAACCTGAC CTGTGAAGAA CTGGCAGAAA GTGATGATGT TAACTACAGA 
ACCTTCATTG AAGAAAAAAT GACTGCAGCC TGTGTTCGGA AATGCCATAT ATGTGCTACA 
GAACTCATCA AAGCAGAAGG GTGCAACAGA TTATCATGTC GCTGTGGGGC ACAAATATGC 
TACTTGTGTC GAGCTCCTAT CAATGGCTAT GACCACTTCT GTCAGCACCC ACATTCTCCT 
GGTGCACCAT GCCGGGAGTG TACCCGTTGC TCTTTGTGGA CTGATCCATC TCAAGATGAT 
GAGAGATTAA TCCTGGAGAT CCAAAGAGAT GCAGAGAATG AACAAAGGAG CCGAAAAGGC 
GAGAAAGCTG TCAAAAGAAT AGGACCTCCT ATTCAGAAGA ACCCTTCCAA GTCTTCAAGG 
ATAGAGACAG TGCCAAGAGA ACGACCCTTA AATCAGCCGC TGATACCTCA GTTTCCCTTA 
ATGCACCCAG CTTTTCCTCT TCCCCCTGTC CAGCCAATGT ATAATAACTT CCAATTGAAT 
TTAGGAGCCC AACATGCCCC ATATGTGCCT CCACTAGCCA ACATTCGGTT GAACTATAAT 
GTTCACCCAA TTGACATGGA TTTAGAGCCG CACTTGCCTA TGCATTTTGG ACCACAGCCT 
CGGCGTGAGT TGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

rnf216 ( XM_018097114.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_017952603.1 Xenopus tropicalis ring finger protein 216 (rnf216), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OXa46588
Clone ID Related Accession (Same CDS sequence)	XM_018097114.1
Accession Version	XM_018097114.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2319bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1473177600000
Organism	Xenopus tropicalis(tropical clawed frog)
Product	E3 ubiquitin-protein ligase RNF216 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030685.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGTCAC AGGATGAGAT GGCTGGAATA AATCCTTTAT GTCATAGAGG AAAAGACTGG 
GACACACACA GGAATGATGC ACCAGTCACT ATATCTGATG ACTCTGATGA AGATCTATCT 
TTTGTCCTTT GCAAGCCAGC ACAGGACTAT GAGGAAGATG AAGATGATGA TGATGATGAT 
GATGTTGTCA TTTTAGAGGA AAGACAACTA AACCACCGTC CTGACCTCAT TCGACCAGCG 
GCTGCGTGGC ACAGTGGGAA CCCATCAGTT AGTGGAAGTC GTCGGCCTTT GTGCAGGAAA 
AGTCTACATG AACAGCATTC TAGAAGACCA GCTTTTCCCA GGACCCATCT AGTGGAGGAT 
GAAATTCCAG GACCCTTATC CCCTCCGATG GCCCATCCTT CAGATGCACC AGAAAAAGAA 
AATCATACAG CAAGTGACCT GCCAAAAAGA GCAACAGGAC CAACTGAGTT AAACCCTGGT 
AATCTGCCTG ATCAGGGAGC TGCAGGACTT GACTATGAGC TGATATCTGA CCTCATTAAG 
GAAACTGAAG CAAGATTTCC AGATGTTAAA CAAGAGTATG TGAAAGAACT GATTCTCTCC 
AAACAATGCT ACGATTTAAA TGTCATATGT AACACCCTTC TGGAAGATCC TGACTACCCA 
AAGAGAGATG GTTTGGCTGT TCTAAATAAG AGTAGCCGTT TACTTACCAG TCATGCGGAT 
CCCAAGGTGA CCATGGTGGA CTATTTTAAT TTTTCACAAC TCGCTCCCCT AGATCAACGG 
TGCCTAATTC AAGCATCAGA CCTCCTAATG GCTGATTTTA AAATGCTCAG CAGTCAAGAT 
ATTAAGTGGG CTTTGCATGA ACTTAAAGGA CACTATGCCA TAACCAGAAA GGCTTTTTCT 
GATGCTATCA AACAGTGGCG GGAGTTGTCT CCAGACCCAG GTGGGAAGCG CAAAAAAAGA 
AAAGAGATGA ACCAGTTTTC CTATATTGAT TTCATATTTG AGCAAGCTCC ACCGCTCCGT 
CCGTCGTCCC TCTCCCGCTC CCTGCATCGG GGGCCAGGAA ATGTAACTAT TGAGAAGCGA 
ATGTACTTTT TGGAAAACAA GAGACGGCAT TACAAAACAT ATGACAAAAA TTCACTACTC 
CCTGCTGTGC GAGAGGAACT AGAATTCTTT GAACGAAAGA TGAAGGAGAT GGCAGAGCAT 
GAAGATTTCC TTCTTGCTTT ACAAGTGAAT GAGGAACAAT ATGAGCAGGA TGGCCAGATG 
ATCGAGTGTC GCTGTTGCTA CGGTTCATTT GCCTTTGAAG AGTTGACTCA ATGTGCAGAT 
GGGCATTTGT TCTGCAAGGA GTGCCTCATA AAATATGCAC AGGAGGCTGC CTTTGGCTCT 
GGGCAGCCCA AACTGATCTG CATGGAAGGC ACTTGCCCCT GTGAGTTTCC AACAAGTGAA 
GTAGAAAAAG TTCTTCCAGA GAATATTCTT CAAAAATATT ATGAAAGGAA AGCTGAAGAA 
GACATCTCTG CAGCATGTGC AGATCAGCTG GTTCGGTGTC CATCTTGCAG TTTCCCTGCA 
CTTCTTGATA AAGATGTGGA TAGATTTAGC TGCCCAAACC CTCATTGTCG AAAAGAAACA 
TGTAAGAAAT GTCAGAAGTT GTGGAAGGAA CATGTAAACC TGACCTGTGA AGAACTGGCA 
GAAAGTGATG ATGTTAACTA CAGAACCTTC ATTGAAGAAA AAATGACTGC AGCCTGTGTT 
CGGAAATGCC ATATATGTGC TACAGAACTC ATCAAAGCAG AAGGGTGCAA CAGATTATCA 
TGTCGCTGTG GGGCACAAAT ATGCTACTTG TGTCGAGCTC CTATCAATGG CTATGACCAC 
TTCTGTCAGC ACCCACATTC TCCTGGTGCA CCATGCCGGG AGTGTACCCG TTGCTCTTTG 
TGGACTGATC CATCTCAAGA TGATGAGAGA TTAATCCTGG AGATCCAAAG AGATGCAGAG 
AATGAACAAA GGAGCCGAAA AGGCGAGAAA GCTGTCAAAA GAATAGGACC TCCTATTCAG 
AAGAACCCTT CCAAGTCTTC AAGGATAGAG ACAGTGCCAA GAGAACGACC CTTAAATCAG 
CCGCTGATAC CTCAGTTTCC CTTAATGCAC CCAGCTTTTC CTCTTCCCCC TGTCCAGCCA 
ATGTATAATA ACTTCCAATT GAATTTAGGA GCCCAACATG CCCCATATGT GCCTCCACTA 
GCCAACATTC GGTTGAACTA TAATGTTCAC CCAATTGACA TGGATTTAGA GCCGCACTTG 
CCTATGCATT TTGGACCACA GCCTCGGCGT GAGTTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017952603.1
CDS	231..2549
Translation	MESQDEMAGINPLCHRGKDWDTHRNDAPVTISDDSDEDLSFVLCKPAQDYEEDEDDDDDDDVVILEERQLNHRPDLIRPAAAWHSGNPSVSGSRRPLCRKSLHEQHSRRPAFPRTHLVEDEIPGPLSPPMAHPSDAPEKENHTASDLPKRATGPTELNPGNLPDQGAAGLDYELISDLIKETEARFPDVKQEYVKELILSKQCYDLNVICNTLLEDPDYPKRDGLAVLNKSSRLLTSHADPKVTMVDYFNFSQLAPLDQRCLIQASDLLMADFKMLSSQDIKWALHELKGHYAITRKAFSDAIKQWRELSPDPGGKRKKRKEMNQFSYIDFIFEQAPPLRPSSLSRSLHRGPGNVTIEKRMYFLENKRRHYKTYDKNSLLPAVREELEFFERKMKEMAEHEDFLLALQVNEEQYEQDGQMIECRCCYGSFAFEELTQCADGHLFCKECLIKYAQEAAFGSGQPKLICMEGTCPCEFPTSEVEKVLPENILQKYYERKAEEDISAACADQLVRCPSCSFPALLDKDVDRFSCPNPHCRKETCKKCQKLWKEHVNLTCEELAESDDVNYRTFIEEKMTAACVRKCHICATELIKAEGCNRLSCRCGAQICYLCRAPINGYDHFCQHPHSPGAPCRECTRCSLWTDPSQDDERLILEIQRDAENEQRSRKGEKAVKRIGPPIQKNPSKSSRIETVPRERPLNQPLIPQFPLMHPAFPLPPVQPMYNNFQLNLGAQHAPYVPPLANIRLNYNVHPIDMDLEPHLPMHFGPQPRREL

Target ORF information:

RefSeq Version	XM_018097114.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis ring finger protein 216 (rnf216), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_018097114.1

ORF Insert Sequence:

ATGGAGTCAC AGGATGAGAT GGCTGGAATA AATCCTTTAT GTCATAGAGG AAAAGACTGG 
GACACACACA GGAATGATGC ACCAGTCACT ATATCTGATG ACTCTGATGA AGATCTATCT 
TTTGTCCTTT GCAAGCCAGC ACAGGACTAT GAGGAAGATG AAGATGATGA TGATGATGAT 
GATGTTGTCA TTTTAGAGGA AAGACAACTA AACCACCGTC CTGACCTCAT TCGACCAGCG 
GCTGCGTGGC ACAGTGGGAA CCCATCAGTT AGTGGAAGTC GTCGGCCTTT GTGCAGGAAA 
AGTCTACATG AACAGCATTC TAGAAGACCA GCTTTTCCCA GGACCCATCT AGTGGAGGAT 
GAAATTCCAG GACCCTTATC CCCTCCGATG GCCCATCCTT CAGATGCACC AGAAAAAGAA 
AATCATACAG CAAGTGACCT GCCAAAAAGA GCAACAGGAC CAACTGAGTT AAACCCTGGT 
AATCTGCCTG ATCAGGGAGC TGCAGGACTT GACTATGAGC TGATATCTGA CCTCATTAAG 
GAAACTGAAG CAAGATTTCC AGATGTTAAA CAAGAGTATG TGAAAGAACT GATTCTCTCC 
AAACAATGCT ACGATTTAAA TGTCATATGT AACACCCTTC TGGAAGATCC TGACTACCCA 
AAGAGAGATG GTTTGGCTGT TCTAAATAAG AGTAGCCGTT TACTTACCAG TCATGCGGAT 
CCCAAGGTGA CCATGGTGGA CTATTTTAAT TTTTCACAAC TCGCTCCCCT AGATCAACGG 
TGCCTAATTC AAGCATCAGA CCTCCTAATG GCTGATTTTA AAATGCTCAG CAGTCAAGAT 
ATTAAGTGGG CTTTGCATGA ACTTAAAGGA CACTATGCCA TAACCAGAAA GGCTTTTTCT 
GATGCTATCA AACAGTGGCG GGAGTTGTCT CCAGACCCAG GTGGGAAGCG CAAAAAAAGA 
AAAGAGATGA ACCAGTTTTC CTATATTGAT TTCATATTTG AGCAAGCTCC ACCGCTCCGT 
CCGTCGTCCC TCTCCCGCTC CCTGCATCGG GGGCCAGGAA ATGTAACTAT TGAGAAGCGA 
ATGTACTTTT TGGAAAACAA GAGACGGCAT TACAAAACAT ATGACAAAAA TTCACTACTC 
CCTGCTGTGC GAGAGGAACT AGAATTCTTT GAACGAAAGA TGAAGGAGAT GGCAGAGCAT 
GAAGATTTCC TTCTTGCTTT ACAAGTGAAT GAGGAACAAT ATGAGCAGGA TGGCCAGATG 
ATCGAGTGTC GCTGTTGCTA CGGTTCATTT GCCTTTGAAG AGTTGACTCA ATGTGCAGAT 
GGGCATTTGT TCTGCAAGGA GTGCCTCATA AAATATGCAC AGGAGGCTGC CTTTGGCTCT 
GGGCAGCCCA AACTGATCTG CATGGAAGGC ACTTGCCCCT GTGAGTTTCC AACAAGTGAA 
GTAGAAAAAG TTCTTCCAGA GAATATTCTT CAAAAATATT ATGAAAGGAA AGCTGAAGAA 
GACATCTCTG CAGCATGTGC AGATCAGCTG GTTCGGTGTC CATCTTGCAG TTTCCCTGCA 
CTTCTTGATA AAGATGTGGA TAGATTTAGC TGCCCAAACC CTCATTGTCG AAAAGAAACA 
TGTAAGAAAT GTCAGAAGTT GTGGAAGGAA CATGTAAACC TGACCTGTGA AGAACTGGCA 
GAAAGTGATG ATGTTAACTA CAGAACCTTC ATTGAAGAAA AAATGACTGC AGCCTGTGTT 
CGGAAATGCC ATATATGTGC TACAGAACTC ATCAAAGCAG AAGGGTGCAA CAGATTATCA 
TGTCGCTGTG GGGCACAAAT ATGCTACTTG TGTCGAGCTC CTATCAATGG CTATGACCAC 
TTCTGTCAGC ACCCACATTC TCCTGGTGCA CCATGCCGGG AGTGTACCCG TTGCTCTTTG 
TGGACTGATC CATCTCAAGA TGATGAGAGA TTAATCCTGG AGATCCAAAG AGATGCAGAG 
AATGAACAAA GGAGCCGAAA AGGCGAGAAA GCTGTCAAAA GAATAGGACC TCCTATTCAG 
AAGAACCCTT CCAAGTCTTC AAGGATAGAG ACAGTGCCAA GAGAACGACC CTTAAATCAG 
CCGCTGATAC CTCAGTTTCC CTTAATGCAC CCAGCTTTTC CTCTTCCCCC TGTCCAGCCA 
ATGTATAATA ACTTCCAATT GAATTTAGGA GCCCAACATG CCCCATATGT GCCTCCACTA 
GCCAACATTC GGTTGAACTA TAATGTTCAC CCAATTGACA TGGATTTAGA GCCGCACTTG 
CCTATGCATT TTGGACCACA GCCTCGGCGT GAGTTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

rnf216 ( XM_012970438.3 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012825892.2 Xenopus tropicalis ring finger protein 216 (rnf216), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OXa66045
Clone ID Related Accession (Same CDS sequence)	XM_012970438.3
Accession Version	XM_012970438.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2661bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1576512000000
Organism	Xenopus tropicalis(tropical clawed frog)
Product	E3 ubiquitin-protein ligase RNF216 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030685.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012970438.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGTCAC AGGATGAGAT GGCTGGAATA AATCCTTTAT GTCATAGAGG AAAAGACTGG 
GACACACACA GGAATGATGC ACCAGTCACT ATATCTGATG ACTCTGATGA AGATCTATCT 
TTTGTCCTTT GCAAGCCAGC ACAGGACTAT GAGGAAGATG AAGATGATGA TGATGATGAT 
GATGTTGTCA TTTTAGAGGA AAGACAACTA AACCACCGTC CTGACCTCAT TCGACCAGCG 
GCTGCGTGGC ACAGTGGGAA CCCATCAGTT AGTGGAAGTC GTCGGCCTTT GTGCAGGAAA 
AGTCTACATG AACAGCATTC TAGAAGTAAT GGGAAGGCTG AATTTAAAGA AGAGATAGAT 
AGAGATATTG TGGATGGAAC CCTTTTGAGC AACAGAGTTC TAGACAGCGA AGAAGATCTC 
TATATAACTC CCACAAAATC TGTTTGCGAT TCTTCGGGTC CTTCTATAAA TAAAGAGACT 
ATTTGTCACA AATCTGTTGG TAAACATGTT GAACCAGTAC CAGGTATTGT GTTGCCATCT 
GTACTTAAGA ACTCTCTTTC CGAACACCCA TTTCATCAGT CTAGTCTCCA GAAAGGATTT 
ACTATAAATT GCCATGCACC AGTGCAAAAA TTAGTAATGA ACAACGATGT TCAAGATGAC 
TTCCTAGGAC CAGCTTTTCC CAGGACCCAT CTAGTGGAGG ATGAAATTCC AGGACCCTTA 
TCCCCTCCGA TGGCCCATCC TTCAGATGCA CCAGAAAAAG AAAATCATAC AGCAAGTGAC 
CTGCCAAAAA GAGCAACAGG ACCAACTGAG TTAAACCCTG GTAATCTGCC TGATCAGGGA 
GCTGCAGGAC TTGACTATGA GCTGATATCT GACCTCATTA AGGAAACTGA AGCAAGATTT 
CCAGATGTTA AACAAGAGTA TGTGAAAGAA CTGATTCTCT CCAAACAATG CTACGATTTA 
AATGTCATAT GTAACACCCT TCTGGAAGAT CCTGACTACC CAAAGAGAGA TGGTTTGGCT 
GTTCTAAATA AGAGTAGCCG TTTACTTACC AGTCATGCGG ATCCCAAGGT GACCATGGTG 
GACTATTTTA ATTTTTCACA ACTCGCTCCC CTAGATCAAC GGTGCCTAAT TCAAGCATCA 
GACCTCCTAA TGGCTGATTT TAAAATGCTC AGCAGTCAAG ATATTAAGTG GGCTTTGCAT 
GAACTTAAAG GACACTATGC CATAACCAGA AAGGCTTTTT CTGATGCTAT CAAACAGTGG 
CGGGAGTTGT CTCCAGACCC AGGTGGGAAG CGCAAAAAAA GAAAAGAGAT GAACCAGTTT 
TCCTATATTG ATTTCATATT TGAGCAAGCT CCACCGCTCC GTCCGTCGTC CCTCTCCCGC 
TCCCTGCATC GGGGGCCAGG AAATGTAACT ATTGAGAAGC GAATGTACTT TTTGGAAAAC 
AAGAGACGGC ATTACAAAAC ATATGACAAA AATTCACTAC TCCCTGCTGT GCGAGAGGAA 
CTAGAATTCT TTGAACGAAA GATGAAGGAG ATGGCAGAGC ATGAAGATTT CCTTCTTGCT 
TTACAAGTGA ATGAGGAACA ATATGAGCAG GATGGCCAGA TGATCGAGTG TCGCTGTTGC 
TACGGTTCAT TTGCCTTTGA AGAGTTGACT CAATGTGCAG ATGGGCATTT GTTCTGCAAG 
GAGTGCCTCA TAAAATATGC ACAGGAGGCT GCCTTTGGCT CTGGGCAGCC CAAACTGATC 
TGCATGGAAG GCACTTGCCC CTGTGAGTTT CCAACAAGTG AAGTAGAAAA AGTTCTTCCA 
GAGAATATTC TTCAAAAATA TTATGAAAGG AAAGCTGAAG AAGACATCTC TGCAGCATGT 
GCAGATCAGC TGGTTCGGTG TCCATCTTGC AGTTTCCCTG CACTTCTTGA TAAAGATGTG 
GATAGATTTA GCTGCCCAAA CCCTCATTGT CGAAAAGAAA CATGTAAGAA ATGTCAGAAG 
TTGTGGAAGG AACATGTAAA CCTGACCTGT GAAGAACTGG CAGAAAGTGA TGATGTTAAC 
TACAGAACCT TCATTGAAGA AAAAATGACT GCAGCCTGTG TTCGGAAATG CCATATATGT 
GCTACAGAAC TCATCAAAGC AGAAGGGTGC AACAGATTAT CATGCCGCTG TGGGGCACAA 
ATATGCTACT TGTGTCGAGC TCCTATCAAT GGCTATGACC ACTTCTGTCA GCATCCACAT 
TCTCCTGGTG CACCATGCCG GGAGTGTACC CGTTGCTCTT TGTGGACTGA TCCATCGCAA 
GATGATGAGA GATTAATCCT GGAGATCCAA AGAGATGCAG AGAATGAACA AAGGAGCCGA 
AAAGGCGAGA AAGCTGTCAA AAGAATAGGA CCTCCTATTC AGAAGAACCC TTCCAAGTCT 
TCAAGGATAG AGACAGTGCC AAGAGAACGA CCCTTAAATC AGCCGCTGAT ACCTCAGTTT 
CCCTTAATGC ACCCAGCTTT TCCTCTTCCC CCTGTCCAGC CAATGTATAA TAACTTCCAA 
TTGAATTTAG GAGCCCAACA TGCCCCATAT GTGCCTCCAC TAGCCAACAT TCGGTTGAAC 
TATAATGTTC ACCCAATTGA CATGGATTTA GAGCCGCACT TGCCTATGCA TTTTGGACCA 
CAGCCTCGGC GTGAGTTGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012825892.2
CDS	208..2868
Translation	MESQDEMAGINPLCHRGKDWDTHRNDAPVTISDDSDEDLSFVLCKPAQDYEEDEDDDDDDDVVILEERQLNHRPDLIRPAAAWHSGNPSVSGSRRPLCRKSLHEQHSRSNGKAEFKEEIDRDIVDGTLLSNRVLDSEEDLYITPTKSVCDSSGPSINKETICHKSVGKHVEPVPGIVLPSVLKNSLSEHPFHQSSLQKGFTINCHAPVQKLVMNNDVQDDFLGPAFPRTHLVEDEIPGPLSPPMAHPSDAPEKENHTASDLPKRATGPTELNPGNLPDQGAAGLDYELISDLIKETEARFPDVKQEYVKELILSKQCYDLNVICNTLLEDPDYPKRDGLAVLNKSSRLLTSHADPKVTMVDYFNFSQLAPLDQRCLIQASDLLMADFKMLSSQDIKWALHELKGHYAITRKAFSDAIKQWRELSPDPGGKRKKRKEMNQFSYIDFIFEQAPPLRPSSLSRSLHRGPGNVTIEKRMYFLENKRRHYKTYDKNSLLPAVREELEFFERKMKEMAEHEDFLLALQVNEEQYEQDGQMIECRCCYGSFAFEELTQCADGHLFCKECLIKYAQEAAFGSGQPKLICMEGTCPCEFPTSEVEKVLPENILQKYYERKAEEDISAACADQLVRCPSCSFPALLDKDVDRFSCPNPHCRKETCKKCQKLWKEHVNLTCEELAESDDVNYRTFIEEKMTAACVRKCHICATELIKAEGCNRLSCRCGAQICYLCRAPINGYDHFCQHPHSPGAPCRECTRCSLWTDPSQDDERLILEIQRDAENEQRSRKGEKAVKRIGPPIQKNPSKSSRIETVPRERPLNQPLIPQFPLMHPAFPLPPVQPMYNNFQLNLGAQHAPYVPPLANIRLNYNVHPIDMDLEPHLPMHFGPQPRREL

Target ORF information:

RefSeq Version	XM_012970438.3
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis ring finger protein 216 (rnf216), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012970438.3

ORF Insert Sequence:

ATGGAGTCAC AGGATGAGAT GGCTGGAATA AATCCTTTAT GTCATAGAGG AAAAGACTGG 
GACACACACA GGAATGATGC ACCAGTCACT ATATCTGATG ACTCTGATGA AGATCTATCT 
TTTGTCCTTT GCAAGCCAGC ACAGGACTAT GAGGAAGATG AAGATGATGA TGATGATGAT 
GATGTTGTCA TTTTAGAGGA AAGACAACTA AACCACCGTC CTGACCTCAT TCGACCAGCG 
GCTGCGTGGC ACAGTGGGAA CCCATCAGTT AGTGGAAGTC GTCGGCCTTT GTGCAGGAAA 
AGTCTACATG AACAGCATTC TAGAAGTAAT GGGAAGGCTG AATTTAAAGA AGAGATAGAT 
AGAGATATTG TGGATGGAAC CCTTTTGAGC AACAGAGTTC TAGACAGCGA AGAAGATCTC 
TATATAACTC CCACAAAATC TGTTTGCGAT TCTTCGGGTC CTTCTATAAA TAAAGAGACT 
ATTTGTCACA AATCTGTTGG TAAACATGTT GAACCAGTAC CAGGTATTGT GTTGCCATCT 
GTACTTAAGA ACTCTCTTTC CGAACACCCA TTTCATCAGT CTAGTCTCCA GAAAGGATTT 
ACTATAAATT GCCATGCACC AGTGCAAAAA TTAGTAATGA ACAACGATGT TCAAGATGAC 
TTCCTAGGAC CAGCTTTTCC CAGGACCCAT CTAGTGGAGG ATGAAATTCC AGGACCCTTA 
TCCCCTCCGA TGGCCCATCC TTCAGATGCA CCAGAAAAAG AAAATCATAC AGCAAGTGAC 
CTGCCAAAAA GAGCAACAGG ACCAACTGAG TTAAACCCTG GTAATCTGCC TGATCAGGGA 
GCTGCAGGAC TTGACTATGA GCTGATATCT GACCTCATTA AGGAAACTGA AGCAAGATTT 
CCAGATGTTA AACAAGAGTA TGTGAAAGAA CTGATTCTCT CCAAACAATG CTACGATTTA 
AATGTCATAT GTAACACCCT TCTGGAAGAT CCTGACTACC CAAAGAGAGA TGGTTTGGCT 
GTTCTAAATA AGAGTAGCCG TTTACTTACC AGTCATGCGG ATCCCAAGGT GACCATGGTG 
GACTATTTTA ATTTTTCACA ACTCGCTCCC CTAGATCAAC GGTGCCTAAT TCAAGCATCA 
GACCTCCTAA TGGCTGATTT TAAAATGCTC AGCAGTCAAG ATATTAAGTG GGCTTTGCAT 
GAACTTAAAG GACACTATGC CATAACCAGA AAGGCTTTTT CTGATGCTAT CAAACAGTGG 
CGGGAGTTGT CTCCAGACCC AGGTGGGAAG CGCAAAAAAA GAAAAGAGAT GAACCAGTTT 
TCCTATATTG ATTTCATATT TGAGCAAGCT CCACCGCTCC GTCCGTCGTC CCTCTCCCGC 
TCCCTGCATC GGGGGCCAGG AAATGTAACT ATTGAGAAGC GAATGTACTT TTTGGAAAAC 
AAGAGACGGC ATTACAAAAC ATATGACAAA AATTCACTAC TCCCTGCTGT GCGAGAGGAA 
CTAGAATTCT TTGAACGAAA GATGAAGGAG ATGGCAGAGC ATGAAGATTT CCTTCTTGCT 
TTACAAGTGA ATGAGGAACA ATATGAGCAG GATGGCCAGA TGATCGAGTG TCGCTGTTGC 
TACGGTTCAT TTGCCTTTGA AGAGTTGACT CAATGTGCAG ATGGGCATTT GTTCTGCAAG 
GAGTGCCTCA TAAAATATGC ACAGGAGGCT GCCTTTGGCT CTGGGCAGCC CAAACTGATC 
TGCATGGAAG GCACTTGCCC CTGTGAGTTT CCAACAAGTG AAGTAGAAAA AGTTCTTCCA 
GAGAATATTC TTCAAAAATA TTATGAAAGG AAAGCTGAAG AAGACATCTC TGCAGCATGT 
GCAGATCAGC TGGTTCGGTG TCCATCTTGC AGTTTCCCTG CACTTCTTGA TAAAGATGTG 
GATAGATTTA GCTGCCCAAA CCCTCATTGT CGAAAAGAAA CATGTAAGAA ATGTCAGAAG 
TTGTGGAAGG AACATGTAAA CCTGACCTGT GAAGAACTGG CAGAAAGTGA TGATGTTAAC 
TACAGAACCT TCATTGAAGA AAAAATGACT GCAGCCTGTG TTCGGAAATG CCATATATGT 
GCTACAGAAC TCATCAAAGC AGAAGGGTGC AACAGATTAT CATGCCGCTG TGGGGCACAA 
ATATGCTACT TGTGTCGAGC TCCTATCAAT GGCTATGACC ACTTCTGTCA GCATCCACAT 
TCTCCTGGTG CACCATGCCG GGAGTGTACC CGTTGCTCTT TGTGGACTGA TCCATCGCAA 
GATGATGAGA GATTAATCCT GGAGATCCAA AGAGATGCAG AGAATGAACA AAGGAGCCGA 
AAAGGCGAGA AAGCTGTCAA AAGAATAGGA CCTCCTATTC AGAAGAACCC TTCCAAGTCT 
TCAAGGATAG AGACAGTGCC AAGAGAACGA CCCTTAAATC AGCCGCTGAT ACCTCAGTTT 
CCCTTAATGC ACCCAGCTTT TCCTCTTCCC CCTGTCCAGC CAATGTATAA TAACTTCCAA 
TTGAATTTAG GAGCCCAACA TGCCCCATAT GTGCCTCCAC TAGCCAACAT TCGGTTGAAC 
TATAATGTTC ACCCAATTGA CATGGATTTA GAGCCGCACT TGCCTATGCA TTTTGGACCA 
CAGCCTCGGC GTGAGTTGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

rnf216 ( XM_012970439.3 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012825893.2 Xenopus tropicalis ring finger protein 216 (rnf216), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OXa66046
Clone ID Related Accession (Same CDS sequence)	XM_012970439.3
Accession Version	XM_012970439.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2610bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1576512000000
Organism	Xenopus tropicalis(tropical clawed frog)
Product	E3 ubiquitin-protein ligase RNF216 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030685.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012970439.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGTCAC AGGATGAGAT GGCTGGAATA AATCCTTTAT GTCATAGAGG AAAAGACTGG 
GACACACACA GGAATGATGC ACCAGTCACT ATATCTGATG ACTCTGATGA AGATCTATCT 
TTTGTCCTTT GCAAGCCAGC ACAGGACTAT GAGGAAGATG AAGATGATGA TGATGATGAT 
GATGTTGTCA TTTTAGAGGA AAGACAACTA AACCACCGTC CTGACCTCAT TCGACCAGCG 
GCTGCGTGGC ACAGTGGGAA CCCATCAGTT AGTGGAAGTC GTCGGCCTTT GTGCAGGAAA 
AGTCTACATG AACAGCATTC TAGAAGTAAT GGGAAGGCTG AATTTAAAGA AGAGATAGAT 
AGAGATATTG TGGATGGAAC CCTTTTGAGC AACAGAGTTC TAGACAGCGA AGAAGATCTC 
TATATAACTC CCACAAAATC TGTTTGCGAT TCTTCGGGTC CTTCTATAAA TAAAGAGACT 
ATTTGTCACA AATCTGTTGG TAAACATGTT GAACCAGTAC CAGGTATTGT GTTGCCATCT 
GTACTTAAGA ACTCTCTTTC CGAACACCCA TTTCATCAGT CTAGTCTCCA GAAAGGATTT 
ACTATAAATT GCCATGCACC AGTGCAAAAA TTAGTAATGA ACAACGATGT TCAAGATGAC 
TTCCTAGGAC CAGCTTTTCC CAGGACCCAT CTAGTGGAGG ATGAAATTCC AGGACCCTTA 
TCCCCTCCGA TGGCCCATCC TTCAGATGCA CCAGAAAAAG AAAATCATAC AGCAAGTGAC 
CTGCCAAAAA GAGCAACAGG ACCAACTGAG TTAAACCCTG GTAATCTGCC TGATCAGGGA 
GCTGCAGGAC TTGACTATGA GCTGATATCT GACCTCATTA AGGAAACTGA AGCAAGATTT 
CCAGATGTTA AACAAGAGTA TGTGAAAGAA CTGATTCTCT CCAAACAATG CTACGATTTA 
AATGTCATAT GTAACACCCT TCTGGAAGAT CCTGACTACC CAAAGAGAGA TGGTTTGGCT 
GTTCTAAATA AGAGTAGCCG TTTACTTACC AGTCATGCGG ATCCCAAGGT GACCATGGTG 
GACTATTTTA ATTTTTCACA ACTCGCTCCC CTAGATCAAC GGTGCCTAAT TCAAGCATCA 
GACCTCCTAA TGGCTGATTT TAAAATGCTC AGCAGTCAAG ATATTAAGTG GGCTTTGCAT 
GAACTTAAAG GACACTATGC CATAACCAGA AAGGCTTTTT CTGATGCTAT CAAACAGTGG 
CGGGAGTTGT CTCCAGACCC AGGTGGGAAG CGCAAAAAAA GAAAAGAGAT GAACCAGTTT 
TCCTATATTG ATTTCATATT TGAGCAAGGA AATGTAACTA TTGAGAAGCG AATGTACTTT 
TTGGAAAACA AGAGACGGCA TTACAAAACA TATGACAAAA ATTCACTACT CCCTGCTGTG 
CGAGAGGAAC TAGAATTCTT TGAACGAAAG ATGAAGGAGA TGGCAGAGCA TGAAGATTTC 
CTTCTTGCTT TACAAGTGAA TGAGGAACAA TATGAGCAGG ATGGCCAGAT GATCGAGTGT 
CGCTGTTGCT ACGGTTCATT TGCCTTTGAA GAGTTGACTC AATGTGCAGA TGGGCATTTG 
TTCTGCAAGG AGTGCCTCAT AAAATATGCA CAGGAGGCTG CCTTTGGCTC TGGGCAGCCC 
AAACTGATCT GCATGGAAGG CACTTGCCCC TGTGAGTTTC CAACAAGTGA AGTAGAAAAA 
GTTCTTCCAG AGAATATTCT TCAAAAATAT TATGAAAGGA AAGCTGAAGA AGACATCTCT 
GCAGCATGTG CAGATCAGCT GGTTCGGTGT CCATCTTGCA GTTTCCCTGC ACTTCTTGAT 
AAAGATGTGG ATAGATTTAG CTGCCCAAAC CCTCATTGTC GAAAAGAAAC ATGTAAGAAA 
TGTCAGAAGT TGTGGAAGGA ACATGTAAAC CTGACCTGTG AAGAACTGGC AGAAAGTGAT 
GATGTTAACT ACAGAACCTT CATTGAAGAA AAAATGACTG CAGCCTGTGT TCGGAAATGC 
CATATATGTG CTACAGAACT CATCAAAGCA GAAGGGTGCA ACAGATTATC ATGCCGCTGT 
GGGGCACAAA TATGCTACTT GTGTCGAGCT CCTATCAATG GCTATGACCA CTTCTGTCAG 
CATCCACATT CTCCTGGTGC ACCATGCCGG GAGTGTACCC GTTGCTCTTT GTGGACTGAT 
CCATCGCAAG ATGATGAGAG ATTAATCCTG GAGATCCAAA GAGATGCAGA GAATGAACAA 
AGGAGCCGAA AAGGCGAGAA AGCTGTCAAA AGAATAGGAC CTCCTATTCA GAAGAACCCT 
TCCAAGTCTT CAAGGATAGA GACAGTGCCA AGAGAACGAC CCTTAAATCA GCCGCTGATA 
CCTCAGTTTC CCTTAATGCA CCCAGCTTTT CCTCTTCCCC CTGTCCAGCC AATGTATAAT 
AACTTCCAAT TGAATTTAGG AGCCCAACAT GCCCCATATG TGCCTCCACT AGCCAACATT 
CGGTTGAACT ATAATGTTCA CCCAATTGAC ATGGATTTAG AGCCGCACTT GCCTATGCAT 
TTTGGACCAC AGCCTCGGCG TGAGTTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012825893.2
CDS	208..2817
Translation	MESQDEMAGINPLCHRGKDWDTHRNDAPVTISDDSDEDLSFVLCKPAQDYEEDEDDDDDDDVVILEERQLNHRPDLIRPAAAWHSGNPSVSGSRRPLCRKSLHEQHSRSNGKAEFKEEIDRDIVDGTLLSNRVLDSEEDLYITPTKSVCDSSGPSINKETICHKSVGKHVEPVPGIVLPSVLKNSLSEHPFHQSSLQKGFTINCHAPVQKLVMNNDVQDDFLGPAFPRTHLVEDEIPGPLSPPMAHPSDAPEKENHTASDLPKRATGPTELNPGNLPDQGAAGLDYELISDLIKETEARFPDVKQEYVKELILSKQCYDLNVICNTLLEDPDYPKRDGLAVLNKSSRLLTSHADPKVTMVDYFNFSQLAPLDQRCLIQASDLLMADFKMLSSQDIKWALHELKGHYAITRKAFSDAIKQWRELSPDPGGKRKKRKEMNQFSYIDFIFEQGNVTIEKRMYFLENKRRHYKTYDKNSLLPAVREELEFFERKMKEMAEHEDFLLALQVNEEQYEQDGQMIECRCCYGSFAFEELTQCADGHLFCKECLIKYAQEAAFGSGQPKLICMEGTCPCEFPTSEVEKVLPENILQKYYERKAEEDISAACADQLVRCPSCSFPALLDKDVDRFSCPNPHCRKETCKKCQKLWKEHVNLTCEELAESDDVNYRTFIEEKMTAACVRKCHICATELIKAEGCNRLSCRCGAQICYLCRAPINGYDHFCQHPHSPGAPCRECTRCSLWTDPSQDDERLILEIQRDAENEQRSRKGEKAVKRIGPPIQKNPSKSSRIETVPRERPLNQPLIPQFPLMHPAFPLPPVQPMYNNFQLNLGAQHAPYVPPLANIRLNYNVHPIDMDLEPHLPMHFGPQPRREL

Target ORF information:

RefSeq Version	XM_012970439.3
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis ring finger protein 216 (rnf216), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012970439.3

ORF Insert Sequence:

ATGGAGTCAC AGGATGAGAT GGCTGGAATA AATCCTTTAT GTCATAGAGG AAAAGACTGG 
GACACACACA GGAATGATGC ACCAGTCACT ATATCTGATG ACTCTGATGA AGATCTATCT 
TTTGTCCTTT GCAAGCCAGC ACAGGACTAT GAGGAAGATG AAGATGATGA TGATGATGAT 
GATGTTGTCA TTTTAGAGGA AAGACAACTA AACCACCGTC CTGACCTCAT TCGACCAGCG 
GCTGCGTGGC ACAGTGGGAA CCCATCAGTT AGTGGAAGTC GTCGGCCTTT GTGCAGGAAA 
AGTCTACATG AACAGCATTC TAGAAGTAAT GGGAAGGCTG AATTTAAAGA AGAGATAGAT 
AGAGATATTG TGGATGGAAC CCTTTTGAGC AACAGAGTTC TAGACAGCGA AGAAGATCTC 
TATATAACTC CCACAAAATC TGTTTGCGAT TCTTCGGGTC CTTCTATAAA TAAAGAGACT 
ATTTGTCACA AATCTGTTGG TAAACATGTT GAACCAGTAC CAGGTATTGT GTTGCCATCT 
GTACTTAAGA ACTCTCTTTC CGAACACCCA TTTCATCAGT CTAGTCTCCA GAAAGGATTT 
ACTATAAATT GCCATGCACC AGTGCAAAAA TTAGTAATGA ACAACGATGT TCAAGATGAC 
TTCCTAGGAC CAGCTTTTCC CAGGACCCAT CTAGTGGAGG ATGAAATTCC AGGACCCTTA 
TCCCCTCCGA TGGCCCATCC TTCAGATGCA CCAGAAAAAG AAAATCATAC AGCAAGTGAC 
CTGCCAAAAA GAGCAACAGG ACCAACTGAG TTAAACCCTG GTAATCTGCC TGATCAGGGA 
GCTGCAGGAC TTGACTATGA GCTGATATCT GACCTCATTA AGGAAACTGA AGCAAGATTT 
CCAGATGTTA AACAAGAGTA TGTGAAAGAA CTGATTCTCT CCAAACAATG CTACGATTTA 
AATGTCATAT GTAACACCCT TCTGGAAGAT CCTGACTACC CAAAGAGAGA TGGTTTGGCT 
GTTCTAAATA AGAGTAGCCG TTTACTTACC AGTCATGCGG ATCCCAAGGT GACCATGGTG 
GACTATTTTA ATTTTTCACA ACTCGCTCCC CTAGATCAAC GGTGCCTAAT TCAAGCATCA 
GACCTCCTAA TGGCTGATTT TAAAATGCTC AGCAGTCAAG ATATTAAGTG GGCTTTGCAT 
GAACTTAAAG GACACTATGC CATAACCAGA AAGGCTTTTT CTGATGCTAT CAAACAGTGG 
CGGGAGTTGT CTCCAGACCC AGGTGGGAAG CGCAAAAAAA GAAAAGAGAT GAACCAGTTT 
TCCTATATTG ATTTCATATT TGAGCAAGGA AATGTAACTA TTGAGAAGCG AATGTACTTT 
TTGGAAAACA AGAGACGGCA TTACAAAACA TATGACAAAA ATTCACTACT CCCTGCTGTG 
CGAGAGGAAC TAGAATTCTT TGAACGAAAG ATGAAGGAGA TGGCAGAGCA TGAAGATTTC 
CTTCTTGCTT TACAAGTGAA TGAGGAACAA TATGAGCAGG ATGGCCAGAT GATCGAGTGT 
CGCTGTTGCT ACGGTTCATT TGCCTTTGAA GAGTTGACTC AATGTGCAGA TGGGCATTTG 
TTCTGCAAGG AGTGCCTCAT AAAATATGCA CAGGAGGCTG CCTTTGGCTC TGGGCAGCCC 
AAACTGATCT GCATGGAAGG CACTTGCCCC TGTGAGTTTC CAACAAGTGA AGTAGAAAAA 
GTTCTTCCAG AGAATATTCT TCAAAAATAT TATGAAAGGA AAGCTGAAGA AGACATCTCT 
GCAGCATGTG CAGATCAGCT GGTTCGGTGT CCATCTTGCA GTTTCCCTGC ACTTCTTGAT 
AAAGATGTGG ATAGATTTAG CTGCCCAAAC CCTCATTGTC GAAAAGAAAC ATGTAAGAAA 
TGTCAGAAGT TGTGGAAGGA ACATGTAAAC CTGACCTGTG AAGAACTGGC AGAAAGTGAT 
GATGTTAACT ACAGAACCTT CATTGAAGAA AAAATGACTG CAGCCTGTGT TCGGAAATGC 
CATATATGTG CTACAGAACT CATCAAAGCA GAAGGGTGCA ACAGATTATC ATGCCGCTGT 
GGGGCACAAA TATGCTACTT GTGTCGAGCT CCTATCAATG GCTATGACCA CTTCTGTCAG 
CATCCACATT CTCCTGGTGC ACCATGCCGG GAGTGTACCC GTTGCTCTTT GTGGACTGAT 
CCATCGCAAG ATGATGAGAG ATTAATCCTG GAGATCCAAA GAGATGCAGA GAATGAACAA 
AGGAGCCGAA AAGGCGAGAA AGCTGTCAAA AGAATAGGAC CTCCTATTCA GAAGAACCCT 
TCCAAGTCTT CAAGGATAGA GACAGTGCCA AGAGAACGAC CCTTAAATCA GCCGCTGATA 
CCTCAGTTTC CCTTAATGCA CCCAGCTTTT CCTCTTCCCC CTGTCCAGCC AATGTATAAT 
AACTTCCAAT TGAATTTAGG AGCCCAACAT GCCCCATATG TGCCTCCACT AGCCAACATT 
CGGTTGAACT ATAATGTTCA CCCAATTGAC ATGGATTTAG AGCCGCACTT GCCTATGCAT 
TTTGGACCAC AGCCTCGGCG TGAGTTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

rnf216 ( XM_018097114.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_017952603.1 Xenopus tropicalis ring finger protein 216 (rnf216), transcript variant X3, mRNA.

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CloneID	OXa66047
Clone ID Related Accession (Same CDS sequence)	XM_018097114.2
Accession Version	XM_018097114.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2319bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1576512000000
Organism	Xenopus tropicalis(tropical clawed frog)
Product	E3 ubiquitin-protein ligase RNF216 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030685.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_018097114.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGTCAC AGGATGAGAT GGCTGGAATA AATCCTTTAT GTCATAGAGG AAAAGACTGG 
GACACACACA GGAATGATGC ACCAGTCACT ATATCTGATG ACTCTGATGA AGATCTATCT 
TTTGTCCTTT GCAAGCCAGC ACAGGACTAT GAGGAAGATG AAGATGATGA TGATGATGAT 
GATGTTGTCA TTTTAGAGGA AAGACAACTA AACCACCGTC CTGACCTCAT TCGACCAGCG 
GCTGCGTGGC ACAGTGGGAA CCCATCAGTT AGTGGAAGTC GTCGGCCTTT GTGCAGGAAA 
AGTCTACATG AACAGCATTC TAGAAGACCA GCTTTTCCCA GGACCCATCT AGTGGAGGAT 
GAAATTCCAG GACCCTTATC CCCTCCGATG GCCCATCCTT CAGATGCACC AGAAAAAGAA 
AATCATACAG CAAGTGACCT GCCAAAAAGA GCAACAGGAC CAACTGAGTT AAACCCTGGT 
AATCTGCCTG ATCAGGGAGC TGCAGGACTT GACTATGAGC TGATATCTGA CCTCATTAAG 
GAAACTGAAG CAAGATTTCC AGATGTTAAA CAAGAGTATG TGAAAGAACT GATTCTCTCC 
AAACAATGCT ACGATTTAAA TGTCATATGT AACACCCTTC TGGAAGATCC TGACTACCCA 
AAGAGAGATG GTTTGGCTGT TCTAAATAAG AGTAGCCGTT TACTTACCAG TCATGCGGAT 
CCCAAGGTGA CCATGGTGGA CTATTTTAAT TTTTCACAAC TCGCTCCCCT AGATCAACGG 
TGCCTAATTC AAGCATCAGA CCTCCTAATG GCTGATTTTA AAATGCTCAG CAGTCAAGAT 
ATTAAGTGGG CTTTGCATGA ACTTAAAGGA CACTATGCCA TAACCAGAAA GGCTTTTTCT 
GATGCTATCA AACAGTGGCG GGAGTTGTCT CCAGACCCAG GTGGGAAGCG CAAAAAAAGA 
AAAGAGATGA ACCAGTTTTC CTATATTGAT TTCATATTTG AGCAAGCTCC ACCGCTCCGT 
CCGTCGTCCC TCTCCCGCTC CCTGCATCGG GGGCCAGGAA ATGTAACTAT TGAGAAGCGA 
ATGTACTTTT TGGAAAACAA GAGACGGCAT TACAAAACAT ATGACAAAAA TTCACTACTC 
CCTGCTGTGC GAGAGGAACT AGAATTCTTT GAACGAAAGA TGAAGGAGAT GGCAGAGCAT 
GAAGATTTCC TTCTTGCTTT ACAAGTGAAT GAGGAACAAT ATGAGCAGGA TGGCCAGATG 
ATCGAGTGTC GCTGTTGCTA CGGTTCATTT GCCTTTGAAG AGTTGACTCA ATGTGCAGAT 
GGGCATTTGT TCTGCAAGGA GTGCCTCATA AAATATGCAC AGGAGGCTGC CTTTGGCTCT 
GGGCAGCCCA AACTGATCTG CATGGAAGGC ACTTGCCCCT GTGAGTTTCC AACAAGTGAA 
GTAGAAAAAG TTCTTCCAGA GAATATTCTT CAAAAATATT ATGAAAGGAA AGCTGAAGAA 
GACATCTCTG CAGCATGTGC AGATCAGCTG GTTCGGTGTC CATCTTGCAG TTTCCCTGCA 
CTTCTTGATA AAGATGTGGA TAGATTTAGC TGCCCAAACC CTCATTGTCG AAAAGAAACA 
TGTAAGAAAT GTCAGAAGTT GTGGAAGGAA CATGTAAACC TGACCTGTGA AGAACTGGCA 
GAAAGTGATG ATGTTAACTA CAGAACCTTC ATTGAAGAAA AAATGACTGC AGCCTGTGTT 
CGGAAATGCC ATATATGTGC TACAGAACTC ATCAAAGCAG AAGGGTGCAA CAGATTATCA 
TGCCGCTGTG GGGCACAAAT ATGCTACTTG TGTCGAGCTC CTATCAATGG CTATGACCAC 
TTCTGTCAGC ATCCACATTC TCCTGGTGCA CCATGCCGGG AGTGTACCCG TTGCTCTTTG 
TGGACTGATC CATCGCAAGA TGATGAGAGA TTAATCCTGG AGATCCAAAG AGATGCAGAG 
AATGAACAAA GGAGCCGAAA AGGCGAGAAA GCTGTCAAAA GAATAGGACC TCCTATTCAG 
AAGAACCCTT CCAAGTCTTC AAGGATAGAG ACAGTGCCAA GAGAACGACC CTTAAATCAG 
CCGCTGATAC CTCAGTTTCC CTTAATGCAC CCAGCTTTTC CTCTTCCCCC TGTCCAGCCA 
ATGTATAATA ACTTCCAATT GAATTTAGGA GCCCAACATG CCCCATATGT GCCTCCACTA 
GCCAACATTC GGTTGAACTA TAATGTTCAC CCAATTGACA TGGATTTAGA GCCGCACTTG 
CCTATGCATT TTGGACCACA GCCTCGGCGT GAGTTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017952603.1
CDS	209..2527
Translation	MESQDEMAGINPLCHRGKDWDTHRNDAPVTISDDSDEDLSFVLCKPAQDYEEDEDDDDDDDVVILEERQLNHRPDLIRPAAAWHSGNPSVSGSRRPLCRKSLHEQHSRRPAFPRTHLVEDEIPGPLSPPMAHPSDAPEKENHTASDLPKRATGPTELNPGNLPDQGAAGLDYELISDLIKETEARFPDVKQEYVKELILSKQCYDLNVICNTLLEDPDYPKRDGLAVLNKSSRLLTSHADPKVTMVDYFNFSQLAPLDQRCLIQASDLLMADFKMLSSQDIKWALHELKGHYAITRKAFSDAIKQWRELSPDPGGKRKKRKEMNQFSYIDFIFEQAPPLRPSSLSRSLHRGPGNVTIEKRMYFLENKRRHYKTYDKNSLLPAVREELEFFERKMKEMAEHEDFLLALQVNEEQYEQDGQMIECRCCYGSFAFEELTQCADGHLFCKECLIKYAQEAAFGSGQPKLICMEGTCPCEFPTSEVEKVLPENILQKYYERKAEEDISAACADQLVRCPSCSFPALLDKDVDRFSCPNPHCRKETCKKCQKLWKEHVNLTCEELAESDDVNYRTFIEEKMTAACVRKCHICATELIKAEGCNRLSCRCGAQICYLCRAPINGYDHFCQHPHSPGAPCRECTRCSLWTDPSQDDERLILEIQRDAENEQRSRKGEKAVKRIGPPIQKNPSKSSRIETVPRERPLNQPLIPQFPLMHPAFPLPPVQPMYNNFQLNLGAQHAPYVPPLANIRLNYNVHPIDMDLEPHLPMHFGPQPRREL

Target ORF information:

RefSeq Version	XM_018097114.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis ring finger protein 216 (rnf216), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_018097114.2

ORF Insert Sequence:

ATGGAGTCAC AGGATGAGAT GGCTGGAATA AATCCTTTAT GTCATAGAGG AAAAGACTGG 
GACACACACA GGAATGATGC ACCAGTCACT ATATCTGATG ACTCTGATGA AGATCTATCT 
TTTGTCCTTT GCAAGCCAGC ACAGGACTAT GAGGAAGATG AAGATGATGA TGATGATGAT 
GATGTTGTCA TTTTAGAGGA AAGACAACTA AACCACCGTC CTGACCTCAT TCGACCAGCG 
GCTGCGTGGC ACAGTGGGAA CCCATCAGTT AGTGGAAGTC GTCGGCCTTT GTGCAGGAAA 
AGTCTACATG AACAGCATTC TAGAAGACCA GCTTTTCCCA GGACCCATCT AGTGGAGGAT 
GAAATTCCAG GACCCTTATC CCCTCCGATG GCCCATCCTT CAGATGCACC AGAAAAAGAA 
AATCATACAG CAAGTGACCT GCCAAAAAGA GCAACAGGAC CAACTGAGTT AAACCCTGGT 
AATCTGCCTG ATCAGGGAGC TGCAGGACTT GACTATGAGC TGATATCTGA CCTCATTAAG 
GAAACTGAAG CAAGATTTCC AGATGTTAAA CAAGAGTATG TGAAAGAACT GATTCTCTCC 
AAACAATGCT ACGATTTAAA TGTCATATGT AACACCCTTC TGGAAGATCC TGACTACCCA 
AAGAGAGATG GTTTGGCTGT TCTAAATAAG AGTAGCCGTT TACTTACCAG TCATGCGGAT 
CCCAAGGTGA CCATGGTGGA CTATTTTAAT TTTTCACAAC TCGCTCCCCT AGATCAACGG 
TGCCTAATTC AAGCATCAGA CCTCCTAATG GCTGATTTTA AAATGCTCAG CAGTCAAGAT 
ATTAAGTGGG CTTTGCATGA ACTTAAAGGA CACTATGCCA TAACCAGAAA GGCTTTTTCT 
GATGCTATCA AACAGTGGCG GGAGTTGTCT CCAGACCCAG GTGGGAAGCG CAAAAAAAGA 
AAAGAGATGA ACCAGTTTTC CTATATTGAT TTCATATTTG AGCAAGCTCC ACCGCTCCGT 
CCGTCGTCCC TCTCCCGCTC CCTGCATCGG GGGCCAGGAA ATGTAACTAT TGAGAAGCGA 
ATGTACTTTT TGGAAAACAA GAGACGGCAT TACAAAACAT ATGACAAAAA TTCACTACTC 
CCTGCTGTGC GAGAGGAACT AGAATTCTTT GAACGAAAGA TGAAGGAGAT GGCAGAGCAT 
GAAGATTTCC TTCTTGCTTT ACAAGTGAAT GAGGAACAAT ATGAGCAGGA TGGCCAGATG 
ATCGAGTGTC GCTGTTGCTA CGGTTCATTT GCCTTTGAAG AGTTGACTCA ATGTGCAGAT 
GGGCATTTGT TCTGCAAGGA GTGCCTCATA AAATATGCAC AGGAGGCTGC CTTTGGCTCT 
GGGCAGCCCA AACTGATCTG CATGGAAGGC ACTTGCCCCT GTGAGTTTCC AACAAGTGAA 
GTAGAAAAAG TTCTTCCAGA GAATATTCTT CAAAAATATT ATGAAAGGAA AGCTGAAGAA 
GACATCTCTG CAGCATGTGC AGATCAGCTG GTTCGGTGTC CATCTTGCAG TTTCCCTGCA 
CTTCTTGATA AAGATGTGGA TAGATTTAGC TGCCCAAACC CTCATTGTCG AAAAGAAACA 
TGTAAGAAAT GTCAGAAGTT GTGGAAGGAA CATGTAAACC TGACCTGTGA AGAACTGGCA 
GAAAGTGATG ATGTTAACTA CAGAACCTTC ATTGAAGAAA AAATGACTGC AGCCTGTGTT 
CGGAAATGCC ATATATGTGC TACAGAACTC ATCAAAGCAG AAGGGTGCAA CAGATTATCA 
TGCCGCTGTG GGGCACAAAT ATGCTACTTG TGTCGAGCTC CTATCAATGG CTATGACCAC 
TTCTGTCAGC ATCCACATTC TCCTGGTGCA CCATGCCGGG AGTGTACCCG TTGCTCTTTG 
TGGACTGATC CATCGCAAGA TGATGAGAGA TTAATCCTGG AGATCCAAAG AGATGCAGAG 
AATGAACAAA GGAGCCGAAA AGGCGAGAAA GCTGTCAAAA GAATAGGACC TCCTATTCAG 
AAGAACCCTT CCAAGTCTTC AAGGATAGAG ACAGTGCCAA GAGAACGACC CTTAAATCAG 
CCGCTGATAC CTCAGTTTCC CTTAATGCAC CCAGCTTTTC CTCTTCCCCC TGTCCAGCCA 
ATGTATAATA ACTTCCAATT GAATTTAGGA GCCCAACATG CCCCATATGT GCCTCCACTA 
GCCAACATTC GGTTGAACTA TAATGTTCAC CCAATTGACA TGGATTTAGA GCCGCACTTG 
CCTATGCATT TTGGACCACA GCCTCGGCGT GAGTTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Genetic and genomic tools for Xenopus research: The NIH Xenopus initiative.
Developmental dynamics : an official publication of the American Association of Anatomists225(4)384-91(2002 Dec)
Klein SL,Strausberg RL,Wagner L,Pontius J,Clifton SW,Richardson P

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rnf216 cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

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Clones

gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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