kif20a cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

Gene
Clones
gRNAs

Summary

Gene Symbol	kif20a
Entrez Gene ID	496538
Full Name	kinesin family member 20A
Synonyms	mklp2,rab6kifl
General protein information	Preferred Names kinesin family member 20A Names kinesin-like protein KIF20A
Gene Type	protein-coding
Organism	Xenopus tropicalis(tropical clawed frog)

mRNA and Protein(s)

mRNA	Protein	Name
XM_004912879.3	XP_004912936.1	kinesin-like protein KIF20A isoform X2
XM_002936835.4	XP_002936881.1	kinesin-like protein KIF20A isoform X1
XM_004912879.4	XP_004912936.1	kinesin-like protein KIF20A isoform X2
XM_002936835.5	XP_002936881.1	kinesin-like protein KIF20A isoform X1
XM_018092219.1	XP_017947708.1	kinesin-like protein KIF20A isoform X1

Pathways from BioSystems

Homologies

Rattus norvegicus (Norway rat)	Kif20a	NP_001101896.1
Macaca mulatta (Rhesus monkey)	KIF20A	XP_001107307.1
Bos taurus (cattle)	KIF20A	NP_001039753.1
Pan troglodytes (chimpanzee)	KIF20A	XP_001170694.1
Gallus gallus (chicken)	KIF20A	NP_001012801.1
Mus musculus (house mouse)	Kif20a	NP_001159878.1
Xenopus tropicalis (tropical clawed frog)	kif20a	XP_002936881.1
Homo sapiens (human)	KIF20A	NP_005724.1
Danio rerio (zebrafish)	LOC325449	NP_001154942.1

Gene Ontology

The following kif20a gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the kif20a cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OXa07667	XM_002936835.5 Latest version!	Xenopus tropicalis kinesin family member 20A (kif20a), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
OXa07667	XM_018092219.1 Latest version!	Xenopus tropicalis kinesin family member 20A (kif20a), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
OXa07668	XM_004912879.4 Latest version!	Xenopus tropicalis kinesin family member 20A (kif20a), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
View Old Accession Versions >>
OXa07668	XM_004912879.3	Xenopus tropicalis kinesin family member 20A (kif20a), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
OXa07667	XM_002936835.4	Xenopus tropicalis kinesin family member 20A (kif20a), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

kif20a ( XM_002936835.4 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_002936881.1 Xenopus tropicalis kinesin family member 20A (kif20a), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OXa07667
Clone ID Related Accession (Same CDS sequence)	XM_002936835.4 , XM_002936835.5 , XM_018092219.1
Accession Version	XM_002936835.4	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2733bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1473177600000
Organism	Xenopus tropicalis(tropical clawed frog)
Product	kinesin-like protein KIF20A isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030679.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_002936835.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTTAGCA ATGTTGTATC CCCTGGCATG GGACTGCTCT CAGATGATGA GGAGAACCCA 
GTGTTTGAAT CTACTGCAGC TGAATGTGGG GCAGGAATCC GAAAAGCACT TCTGTCTGAC 
TTCTCTGTCA TCTCACCAGG ACTGGGCCAT CCTCCCCAGT CTATGCCTGA GGAATCAAAT 
GACAAAGTCA TGGTTTATGT GAGGATTAGA CCCTTTGTAG ATGGGGAGTT GGAGAAAAAG 
GAGGACCAGG GCTGTGTTGA GGTGGAGAAT GAGGAGACCC TGGTGTTGCG TGCTCCTAAG 
GATTCCTTTG CAATGAAAAA TACAGAACGC GGACTGGGGC AGAGTGTGCA CAAATTTACT 
TTTTCTCAGC TATTTGGACC AGAAGTGGAC CAGAAGCAGT TTTTTGATGG AACTATGCGG 
CAAGTGGTAA AGGATGCTCT GAACGGCCAA AACTGGCTTA TTTATACTTA TGGCGTTACT 
AACTCTGGCA AAACCTTTAC CATACAAGGG ACTAGCAAGG ATAGGGGTAT TCTGCCTCGC 
TCTATTGCAC TTATCTTTAA CAGTGCCCAG GATCGCCTGT ATAAAAGCTC AGACCTCAAA 
CCTCTTTCTA ATGAGGTGAT GTGGCTGGAC AGCAAACAAG TGCGACAGGA AGAACTTAAA 
AAAGTTTCAC TGCTGTCTAA TCTGAGAGAG GAAGAACTTA TGACCCCCCT AAAAAGAAGT 
GGTAATGAAT CCCAGGTCCG GGCTGGCACA AGTGTTAGCT TTGACAGTGG CATTGGGGGA 
CTTTCTTCTA CAAGTCAGTT TGTCAATCAG ACTTGTAGCC AGCTAGAAGA GACCTGTTCA 
CGATGGAATG ACCAAGAAGT AGTATCGCTT CAGGAGGCTG CCGACGTACA ACTCTCCATC 
TGGGTATCCT ATTATGAAAT CTACAATGAA TTTGTTTATG ATTTACTGGA CTTCATGCCT 
TCTGGTCCGA ACCGTAAACG AACCACATTG CGACTGTGTG AGGACAGAAA TGGGAATCCA 
TATGTGAAAG ACCTCACCTG GATAAATGTA CACAGTGCTG AAGAAGCTTG GAAGATCCTA 
AGAGTTGGAA GGAAAAACCA GAGTTTTGCC AGTACACATC TGAACCAAAA CTCAAGCCGG 
AGTCACAGTA TCTTTTCCAT TCGGATTCTC CACATGCAAG GAAAAAATGA TTTGACGCCT 
AAAATAAGCG AGCTTTCTTT CTGTGACTTG GCTGGATCTG AGCGCTGTAA GGATCAGAGA 
AGCGGTGATC GCCTCAAGGA AGCAACTAAC ATTAATACCT CTTTGCACAC TCTGGGCCGC 
TGCATTACAG TATTGAGACA GAACCAACAA CAAAAGTTGC GACAGAACGT GGTCCCTTTC 
AGGGACAGTA AGCTGACCCG CATATTCCAG GCATACTTCA CTGGGCGTGG GAAGTCTTGC 
ATGATTGTAA ACATTAATCA GTGCGCTTCT ACATATGATG AGACTCTTTA TGCCATGAAG 
TTTTCAGCCA TTGCCAGCCA GTTGGTTCAA GCTCCACCAG TTAAAGTGAA CATTCCGTCC 
ATTCAGTCAC TCATCAAGGA AAGCACCATG CTAGCCAATC GCAGTTACAC AGATGAGGAG 
GATGCTGACG AAAATTCTGA TGAAGAAAGC GAGGAAACGG ATATTGCAAA ATTTAACAGA 
GAGGAGTTAT TACAAGTTAT TGAGAACATG AAGGAACTAA TTGTAAAGGA GCGTCAGGAG 
AAGCTGAACA TGGAGATACA AATCCGTAAG GAAGTTTGTA GCGAAATGAT GGATATGATG 
CAACAACAGC AGAAACAGCA TAGTGAGAGC CTGGAAGATG AGCGAGAATT GTTAGAGGAG 
ATATACGAGG GTCGGCTGGA GAACCTTAAA GAATCTCTAA CAAATTATTA CAAACACGAA 
TTGGAGGAAA GAGATGAGAG GATTGAAGAA CTGGAAGCTG CTTTGAAGGA AGCCATGGAA 
AGTGTCCATT CCATGCCAAG CCAGCCGACA CAAAGAGAAG AAAGTCTCTC GGTCAGAAGG 
TCTAAGCGAT TAGCAACAGC TGAGGCATCT AACACAGACA AATTTAAACA AGAGCTCCTG 
AATCTGAAAA TGGAGCTTCA GTCAAAGAAT GAGGAAATTG AAAAGTACAA GAAAATGCAA 
GAGCCTCCGG TCTCTGCCCG ATCATATACA GCTGATGTTG ATAAGAAGAT TGTGGAAGGG 
CAGAGACTTT ATGGTCTGGG CTCTCTTCAC AAGTGCACCG ATTTTCAGCA CACAAGAGCT 
CTTTGGAATG TAAGATTATT GCGCACTGAA TTGCAGAGGT TTGGGGATTC CTTGCAGCAG 
GTGGACAGGG TGTGCTGTCA TACAACTGGA GCTGAGAAAC TGAGGCAAGC TCTTGGTACT 
TGTGATGATA TACTGTCCAA ACAGGAAAGG GCTCTGGCAG AACTTCAAAA TAACATGGTG 
TTGGTGAAAA TGGACCTGAA GAAGAAGGCT GTTTGCATTG CTGAGCAGTA TAACACTGTA 
CAGAAACTAC AGGGAACCAC TTCTGGCTAC AAACGGCTCT GCAATAATGA GAATGTCCAA 
CCAAATAATC CAGAGAAGAA ACCCTTCCTA CGCAACTTCC TGGCAAGGAC CCCAGGAAAG 
AGAGGACTTG CAGAAAACAG TCCCTACAGT CGCATATTGC GCTCACGGCG ATCCCCTGTG 
CTTAAAACTG TTGCTTTTGG CACCACATAC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_002936881.1
CDS	165..2897
Translation	MVSNVVSPGMGLLSDDEENPVFESTAAECGAGIRKALLSDFSVISPGLGHPPQSMPEESNDKVMVYVRIRPFVDGELEKKEDQGCVEVENEETLVLRAPKDSFAMKNTERGLGQSVHKFTFSQLFGPEVDQKQFFDGTMRQVVKDALNGQNWLIYTYGVTNSGKTFTIQGTSKDRGILPRSIALIFNSAQDRLYKSSDLKPLSNEVMWLDSKQVRQEELKKVSLLSNLREEELMTPLKRSGNESQVRAGTSVSFDSGIGGLSSTSQFVNQTCSQLEETCSRWNDQEVVSLQEAADVQLSIWVSYYEIYNEFVYDLLDFMPSGPNRKRTTLRLCEDRNGNPYVKDLTWINVHSAEEAWKILRVGRKNQSFASTHLNQNSSRSHSIFSIRILHMQGKNDLTPKISELSFCDLAGSERCKDQRSGDRLKEATNINTSLHTLGRCITVLRQNQQQKLRQNVVPFRDSKLTRIFQAYFTGRGKSCMIVNINQCASTYDETLYAMKFSAIASQLVQAPPVKVNIPSIQSLIKESTMLANRSYTDEEDADENSDEESEETDIAKFNREELLQVIENMKELIVKERQEKLNMEIQIRKEVCSEMMDMMQQQQKQHSESLEDERELLEEIYEGRLENLKESLTNYYKHELEERDERIEELEAALKEAMESVHSMPSQPTQREESLSVRRSKRLATAEASNTDKFKQELLNLKMELQSKNEEIEKYKKMQEPPVSARSYTADVDKKIVEGQRLYGLGSLHKCTDFQHTRALWNVRLLRTELQRFGDSLQQVDRVCCHTTGAEKLRQALGTCDDILSKQERALAELQNNMVLVKMDLKKKAVCIAEQYNTVQKLQGTTSGYKRLCNNENVQPNNPEKKPFLRNFLARTPGKRGLAENSPYSRILRSRRSPVLKTVAFGTTY

Target ORF information:

RefSeq Version	XM_002936835.4
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis kinesin family member 20A (kif20a), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_002936835.4

ORF Insert Sequence:

ATGGTTAGCA ATGTTGTATC CCCTGGCATG GGACTGCTCT CAGATGATGA GGAGAACCCA 
GTGTTTGAAT CTACTGCAGC TGAATGTGGG GCAGGAATCC GAAAAGCACT TCTGTCTGAC 
TTCTCTGTCA TCTCACCAGG ACTGGGCCAT CCTCCCCAGT CTATGCCTGA GGAATCAAAT 
GACAAAGTCA TGGTTTATGT GAGGATTAGA CCCTTTGTAG ATGGGGAGTT GGAGAAAAAG 
GAGGACCAGG GCTGTGTTGA GGTGGAGAAT GAGGAGACCC TGGTGTTGCG TGCTCCTAAG 
GATTCCTTTG CAATGAAAAA TACAGAACGC GGACTGGGGC AGAGTGTGCA CAAATTTACT 
TTTTCTCAGC TATTTGGACC AGAAGTGGAC CAGAAGCAGT TTTTTGATGG AACTATGCGG 
CAAGTGGTAA AGGATGCTCT GAACGGCCAA AACTGGCTTA TTTATACTTA TGGCGTTACT 
AACTCTGGCA AAACCTTTAC CATACAAGGG ACTAGCAAGG ATAGGGGTAT TCTGCCTCGC 
TCTATTGCAC TTATCTTTAA CAGTGCCCAG GATCGCCTGT ATAAAAGCTC AGACCTCAAA 
CCTCTTTCTA ATGAGGTGAT GTGGCTGGAC AGCAAACAAG TGCGACAGGA AGAACTTAAA 
AAAGTTTCAC TGCTGTCTAA TCTGAGAGAG GAAGAACTTA TGACCCCCCT AAAAAGAAGT 
GGTAATGAAT CCCAGGTCCG GGCTGGCACA AGTGTTAGCT TTGACAGTGG CATTGGGGGA 
CTTTCTTCTA CAAGTCAGTT TGTCAATCAG ACTTGTAGCC AGCTAGAAGA GACCTGTTCA 
CGATGGAATG ACCAAGAAGT AGTATCGCTT CAGGAGGCTG CCGACGTACA ACTCTCCATC 
TGGGTATCCT ATTATGAAAT CTACAATGAA TTTGTTTATG ATTTACTGGA CTTCATGCCT 
TCTGGTCCGA ACCGTAAACG AACCACATTG CGACTGTGTG AGGACAGAAA TGGGAATCCA 
TATGTGAAAG ACCTCACCTG GATAAATGTA CACAGTGCTG AAGAAGCTTG GAAGATCCTA 
AGAGTTGGAA GGAAAAACCA GAGTTTTGCC AGTACACATC TGAACCAAAA CTCAAGCCGG 
AGTCACAGTA TCTTTTCCAT TCGGATTCTC CACATGCAAG GAAAAAATGA TTTGACGCCT 
AAAATAAGCG AGCTTTCTTT CTGTGACTTG GCTGGATCTG AGCGCTGTAA GGATCAGAGA 
AGCGGTGATC GCCTCAAGGA AGCAACTAAC ATTAATACCT CTTTGCACAC TCTGGGCCGC 
TGCATTACAG TATTGAGACA GAACCAACAA CAAAAGTTGC GACAGAACGT GGTCCCTTTC 
AGGGACAGTA AGCTGACCCG CATATTCCAG GCATACTTCA CTGGGCGTGG GAAGTCTTGC 
ATGATTGTAA ACATTAATCA GTGCGCTTCT ACATATGATG AGACTCTTTA TGCCATGAAG 
TTTTCAGCCA TTGCCAGCCA GTTGGTTCAA GCTCCACCAG TTAAAGTGAA CATTCCGTCC 
ATTCAGTCAC TCATCAAGGA AAGCACCATG CTAGCCAATC GCAGTTACAC AGATGAGGAG 
GATGCTGACG AAAATTCTGA TGAAGAAAGC GAGGAAACGG ATATTGCAAA ATTTAACAGA 
GAGGAGTTAT TACAAGTTAT TGAGAACATG AAGGAACTAA TTGTAAAGGA GCGTCAGGAG 
AAGCTGAACA TGGAGATACA AATCCGTAAG GAAGTTTGTA GCGAAATGAT GGATATGATG 
CAACAACAGC AGAAACAGCA TAGTGAGAGC CTGGAAGATG AGCGAGAATT GTTAGAGGAG 
ATATACGAGG GTCGGCTGGA GAACCTTAAA GAATCTCTAA CAAATTATTA CAAACACGAA 
TTGGAGGAAA GAGATGAGAG GATTGAAGAA CTGGAAGCTG CTTTGAAGGA AGCCATGGAA 
AGTGTCCATT CCATGCCAAG CCAGCCGACA CAAAGAGAAG AAAGTCTCTC GGTCAGAAGG 
TCTAAGCGAT TAGCAACAGC TGAGGCATCT AACACAGACA AATTTAAACA AGAGCTCCTG 
AATCTGAAAA TGGAGCTTCA GTCAAAGAAT GAGGAAATTG AAAAGTACAA GAAAATGCAA 
GAGCCTCCGG TCTCTGCCCG ATCATATACA GCTGATGTTG ATAAGAAGAT TGTGGAAGGG 
CAGAGACTTT ATGGTCTGGG CTCTCTTCAC AAGTGCACCG ATTTTCAGCA CACAAGAGCT 
CTTTGGAATG TAAGATTATT GCGCACTGAA TTGCAGAGGT TTGGGGATTC CTTGCAGCAG 
GTGGACAGGG TGTGCTGTCA TACAACTGGA GCTGAGAAAC TGAGGCAAGC TCTTGGTACT 
TGTGATGATA TACTGTCCAA ACAGGAAAGG GCTCTGGCAG AACTTCAAAA TAACATGGTG 
TTGGTGAAAA TGGACCTGAA GAAGAAGGCT GTTTGCATTG CTGAGCAGTA TAACACTGTA 
CAGAAACTAC AGGGAACCAC TTCTGGCTAC AAACGGCTCT GCAATAATGA GAATGTCCAA 
CCAAATAATC CAGAGAAGAA ACCCTTCCTA CGCAACTTCC TGGCAAGGAC CCCAGGAAAG 
AGAGGACTTG CAGAAAACAG TCCCTACAGT CGCATATTGC GCTCACGGCG ATCCCCTGTG 
CTTAAAACTG TTGCTTTTGG CACCACATAC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

kif20a ( XM_002936835.5 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_002936881.1 Xenopus tropicalis kinesin family member 20A (kif20a), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OXa07667
Clone ID Related Accession (Same CDS sequence)	XM_002936835.4 , XM_002936835.5 , XM_018092219.1
Accession Version	XM_002936835.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2733bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1576512000000
Organism	Xenopus tropicalis(tropical clawed frog)
Product	kinesin-like protein KIF20A isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030679.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_002936835.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTTAGCA ATGTTGTATC CCCTGGCATG GGACTGCTCT CAGATGATGA GGAGAACCCA 
GTGTTTGAAT CTACTGCAGC TGAATGTGGG GCAGGAATCC GAAAAGCACT TCTGTCTGAC 
TTCTCTGTCA TCTCACCAGG ACTGGGCCAT CCTCCCCAGT CTATGCCTGA GGAATCAAAT 
GACAAAGTCA TGGTTTATGT GAGGATTAGA CCCTTTGTAG ATGGGGAGTT GGAGAAAAAG 
GAGGACCAGG GCTGTGTTGA GGTGGAGAAT GAGGAGACCC TGGTGTTGCG TGCTCCTAAG 
GATTCCTTTG CAATGAAAAA TACAGAACGC GGACTGGGGC AGAGTGTGCA CAAATTTACT 
TTTTCTCAGC TATTTGGACC AGAAGTGGAC CAGAAGCAGT TTTTTGATGG AACTATGCGG 
CAAGTGGTAA AGGATGCTCT GAACGGCCAA AACTGGCTTA TTTATACTTA TGGCGTTACT 
AACTCTGGCA AAACCTTTAC CATACAAGGG ACTAGCAAGG ATAGGGGTAT TCTGCCTCGC 
TCTATTGCAC TTATCTTTAA CAGTGCCCAG GATCGCCTGT ATAAAAGCTC AGACCTCAAA 
CCTCTTTCTA ATGAGGTGAT GTGGCTGGAC AGCAAACAAG TGCGACAGGA AGAACTTAAA 
AAAGTTTCAC TGCTGTCTAA TCTGAGAGAG GAAGAACTTA TGACCCCCCT AAAAAGAAGT 
GGTAATGAAT CCCAGGTCCG GGCTGGCACA AGTGTTAGCT TTGACAGTGG CATTGGGGGA 
CTTTCTTCTA CAAGTCAGTT TGTCAATCAG ACTTGTAGCC AGCTAGAAGA GACCTGTTCA 
CGATGGAATG ACCAAGAAGT AGTATCGCTT CAGGAGGCTG CCGACGTACA ACTCTCCATC 
TGGGTATCCT ATTATGAAAT CTACAATGAA TTTGTTTATG ATTTACTGGA CTTCATGCCT 
TCTGGTCCGA ACCGTAAACG AACCACATTG CGACTGTGTG AGGACAGAAA TGGGAATCCA 
TATGTGAAAG ACCTCACCTG GATAAATGTA CACAGTGCTG AAGAAGCTTG GAAGATCCTA 
AGAGTTGGAA GGAAAAACCA GAGTTTTGCC AGTACACATC TGAACCAAAA CTCAAGCCGG 
AGTCACAGTA TCTTTTCCAT TCGGATTCTC CACATGCAAG GAAAAAATGA TTTGACGCCT 
AAAATAAGCG AGCTTTCTTT CTGTGACTTG GCTGGATCTG AGCGCTGTAA GGATCAGAGA 
AGCGGTGATC GCCTCAAGGA AGCAACTAAC ATTAATACCT CTTTGCACAC TCTGGGCCGC 
TGCATTACAG TATTGAGACA GAACCAACAA CAAAAGTTGC GACAGAACGT GGTCCCTTTC 
AGGGACAGTA AGCTGACCCG CATATTCCAG GCATACTTCA CTGGGCGTGG GAAGTCTTGC 
ATGATTGTAA ACATTAATCA GTGCGCTTCT ACATATGATG AGACTCTTTA TGCCATGAAG 
TTTTCAGCCA TTGCCAGCCA GTTGGTTCAA GCTCCACCAG TTAAAGTGAA CATTCCGTCC 
ATTCAGTCAC TCATCAAGGA AAGCACCATG CTAGCCAATC GCAGTTACAC AGATGAGGAG 
GATGCTGACG AAAATTCTGA TGAAGAAAGC GAGGAAACGG ATATTGCAAA ATTTAACAGA 
GAGGAGTTAT TACAAGTTAT TGAGAACATG AAGGAACTAA TTGTAAAGGA GCGTCAGGAG 
AAGCTGAACA TGGAGATACA AATCCGTAAG GAAGTTTGTA GCGAAATGAT GGATATGATG 
CAACAACAGC AGAAACAGCA TAGTGAGAGC CTGGAAGATG AGCGAGAATT GTTAGAGGAG 
ATATACGAGG GTCGGCTGGA GAACCTTAAA GAATCTCTAA CAAATTATTA CAAACACGAA 
TTGGAGGAAA GAGATGAGAG GATTGAAGAA CTGGAAGCTG CTTTGAAGGA AGCCATGGAA 
AGTGTCCATT CCATGCCAAG CCAGCCGACA CAAAGAGAAG AAAGTCTCTC GGTCAGAAGG 
TCTAAGCGAT TAGCAACAGC TGAGGCATCT AACACAGACA AATTTAAACA AGAGCTCCTG 
AATCTGAAAA TGGAGCTTCA GTCAAAGAAT GAGGAAATTG AAAAGTACAA GAAAATGCAA 
GAGCCTCCGG TCTCTGCCCG ATCATATACA GCTGATGTTG ATAAGAAGAT TGTGGAAGGG 
CAGAGACTTT ATGGTCTGGG CTCTCTTCAC AAGTGCACCG ATTTTCAGCA CACAAGAGCT 
CTTTGGAATG TAAGATTATT GCGCACTGAA TTGCAGAGGT TTGGGGATTC CTTGCAGCAG 
GTGGACAGGG TGTGCTGTCA TACAACTGGA GCTGAGAAAC TGAGGCAAGC TCTTGGTACT 
TGTGATGATA TACTGTCCAA ACAGGAAAGG GCTCTGGCAG AACTTCAAAA TAACATGGTG 
TTGGTGAAAA TGGACCTGAA GAAGAAGGCT GTTTGCATTG CTGAGCAGTA TAACACTGTA 
CAGAAACTAC AGGGAACCAC TTCTGGCTAC AAACGGCTCT GCAATAATGA GAATGTCCAA 
CCAAATAATC CAGAGAAGAA ACCCTTCCTA CGCAACTTCC TGGCAAGGAC CCCAGGAAAG 
AGAGGACTTG CAGAAAACAG TCCCTACAGT CGCATATTGC GCTCACGGCG ATCCCCTGTG 
CTTAAAACTG TTGCTTTTGG CACCACATAC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_002936881.1
CDS	163..2895
Translation	MVSNVVSPGMGLLSDDEENPVFESTAAECGAGIRKALLSDFSVISPGLGHPPQSMPEESNDKVMVYVRIRPFVDGELEKKEDQGCVEVENEETLVLRAPKDSFAMKNTERGLGQSVHKFTFSQLFGPEVDQKQFFDGTMRQVVKDALNGQNWLIYTYGVTNSGKTFTIQGTSKDRGILPRSIALIFNSAQDRLYKSSDLKPLSNEVMWLDSKQVRQEELKKVSLLSNLREEELMTPLKRSGNESQVRAGTSVSFDSGIGGLSSTSQFVNQTCSQLEETCSRWNDQEVVSLQEAADVQLSIWVSYYEIYNEFVYDLLDFMPSGPNRKRTTLRLCEDRNGNPYVKDLTWINVHSAEEAWKILRVGRKNQSFASTHLNQNSSRSHSIFSIRILHMQGKNDLTPKISELSFCDLAGSERCKDQRSGDRLKEATNINTSLHTLGRCITVLRQNQQQKLRQNVVPFRDSKLTRIFQAYFTGRGKSCMIVNINQCASTYDETLYAMKFSAIASQLVQAPPVKVNIPSIQSLIKESTMLANRSYTDEEDADENSDEESEETDIAKFNREELLQVIENMKELIVKERQEKLNMEIQIRKEVCSEMMDMMQQQQKQHSESLEDERELLEEIYEGRLENLKESLTNYYKHELEERDERIEELEAALKEAMESVHSMPSQPTQREESLSVRRSKRLATAEASNTDKFKQELLNLKMELQSKNEEIEKYKKMQEPPVSARSYTADVDKKIVEGQRLYGLGSLHKCTDFQHTRALWNVRLLRTELQRFGDSLQQVDRVCCHTTGAEKLRQALGTCDDILSKQERALAELQNNMVLVKMDLKKKAVCIAEQYNTVQKLQGTTSGYKRLCNNENVQPNNPEKKPFLRNFLARTPGKRGLAENSPYSRILRSRRSPVLKTVAFGTTY

Target ORF information:

RefSeq Version	XM_002936835.5
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis kinesin family member 20A (kif20a), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_002936835.5

ORF Insert Sequence:

ATGGTTAGCA ATGTTGTATC CCCTGGCATG GGACTGCTCT CAGATGATGA GGAGAACCCA 
GTGTTTGAAT CTACTGCAGC TGAATGTGGG GCAGGAATCC GAAAAGCACT TCTGTCTGAC 
TTCTCTGTCA TCTCACCAGG ACTGGGCCAT CCTCCCCAGT CTATGCCTGA GGAATCAAAT 
GACAAAGTCA TGGTTTATGT GAGGATTAGA CCCTTTGTAG ATGGGGAGTT GGAGAAAAAG 
GAGGACCAGG GCTGTGTTGA GGTGGAGAAT GAGGAGACCC TGGTGTTGCG TGCTCCTAAG 
GATTCCTTTG CAATGAAAAA TACAGAACGC GGACTGGGGC AGAGTGTGCA CAAATTTACT 
TTTTCTCAGC TATTTGGACC AGAAGTGGAC CAGAAGCAGT TTTTTGATGG AACTATGCGG 
CAAGTGGTAA AGGATGCTCT GAACGGCCAA AACTGGCTTA TTTATACTTA TGGCGTTACT 
AACTCTGGCA AAACCTTTAC CATACAAGGG ACTAGCAAGG ATAGGGGTAT TCTGCCTCGC 
TCTATTGCAC TTATCTTTAA CAGTGCCCAG GATCGCCTGT ATAAAAGCTC AGACCTCAAA 
CCTCTTTCTA ATGAGGTGAT GTGGCTGGAC AGCAAACAAG TGCGACAGGA AGAACTTAAA 
AAAGTTTCAC TGCTGTCTAA TCTGAGAGAG GAAGAACTTA TGACCCCCCT AAAAAGAAGT 
GGTAATGAAT CCCAGGTCCG GGCTGGCACA AGTGTTAGCT TTGACAGTGG CATTGGGGGA 
CTTTCTTCTA CAAGTCAGTT TGTCAATCAG ACTTGTAGCC AGCTAGAAGA GACCTGTTCA 
CGATGGAATG ACCAAGAAGT AGTATCGCTT CAGGAGGCTG CCGACGTACA ACTCTCCATC 
TGGGTATCCT ATTATGAAAT CTACAATGAA TTTGTTTATG ATTTACTGGA CTTCATGCCT 
TCTGGTCCGA ACCGTAAACG AACCACATTG CGACTGTGTG AGGACAGAAA TGGGAATCCA 
TATGTGAAAG ACCTCACCTG GATAAATGTA CACAGTGCTG AAGAAGCTTG GAAGATCCTA 
AGAGTTGGAA GGAAAAACCA GAGTTTTGCC AGTACACATC TGAACCAAAA CTCAAGCCGG 
AGTCACAGTA TCTTTTCCAT TCGGATTCTC CACATGCAAG GAAAAAATGA TTTGACGCCT 
AAAATAAGCG AGCTTTCTTT CTGTGACTTG GCTGGATCTG AGCGCTGTAA GGATCAGAGA 
AGCGGTGATC GCCTCAAGGA AGCAACTAAC ATTAATACCT CTTTGCACAC TCTGGGCCGC 
TGCATTACAG TATTGAGACA GAACCAACAA CAAAAGTTGC GACAGAACGT GGTCCCTTTC 
AGGGACAGTA AGCTGACCCG CATATTCCAG GCATACTTCA CTGGGCGTGG GAAGTCTTGC 
ATGATTGTAA ACATTAATCA GTGCGCTTCT ACATATGATG AGACTCTTTA TGCCATGAAG 
TTTTCAGCCA TTGCCAGCCA GTTGGTTCAA GCTCCACCAG TTAAAGTGAA CATTCCGTCC 
ATTCAGTCAC TCATCAAGGA AAGCACCATG CTAGCCAATC GCAGTTACAC AGATGAGGAG 
GATGCTGACG AAAATTCTGA TGAAGAAAGC GAGGAAACGG ATATTGCAAA ATTTAACAGA 
GAGGAGTTAT TACAAGTTAT TGAGAACATG AAGGAACTAA TTGTAAAGGA GCGTCAGGAG 
AAGCTGAACA TGGAGATACA AATCCGTAAG GAAGTTTGTA GCGAAATGAT GGATATGATG 
CAACAACAGC AGAAACAGCA TAGTGAGAGC CTGGAAGATG AGCGAGAATT GTTAGAGGAG 
ATATACGAGG GTCGGCTGGA GAACCTTAAA GAATCTCTAA CAAATTATTA CAAACACGAA 
TTGGAGGAAA GAGATGAGAG GATTGAAGAA CTGGAAGCTG CTTTGAAGGA AGCCATGGAA 
AGTGTCCATT CCATGCCAAG CCAGCCGACA CAAAGAGAAG AAAGTCTCTC GGTCAGAAGG 
TCTAAGCGAT TAGCAACAGC TGAGGCATCT AACACAGACA AATTTAAACA AGAGCTCCTG 
AATCTGAAAA TGGAGCTTCA GTCAAAGAAT GAGGAAATTG AAAAGTACAA GAAAATGCAA 
GAGCCTCCGG TCTCTGCCCG ATCATATACA GCTGATGTTG ATAAGAAGAT TGTGGAAGGG 
CAGAGACTTT ATGGTCTGGG CTCTCTTCAC AAGTGCACCG ATTTTCAGCA CACAAGAGCT 
CTTTGGAATG TAAGATTATT GCGCACTGAA TTGCAGAGGT TTGGGGATTC CTTGCAGCAG 
GTGGACAGGG TGTGCTGTCA TACAACTGGA GCTGAGAAAC TGAGGCAAGC TCTTGGTACT 
TGTGATGATA TACTGTCCAA ACAGGAAAGG GCTCTGGCAG AACTTCAAAA TAACATGGTG 
TTGGTGAAAA TGGACCTGAA GAAGAAGGCT GTTTGCATTG CTGAGCAGTA TAACACTGTA 
CAGAAACTAC AGGGAACCAC TTCTGGCTAC AAACGGCTCT GCAATAATGA GAATGTCCAA 
CCAAATAATC CAGAGAAGAA ACCCTTCCTA CGCAACTTCC TGGCAAGGAC CCCAGGAAAG 
AGAGGACTTG CAGAAAACAG TCCCTACAGT CGCATATTGC GCTCACGGCG ATCCCCTGTG 
CTTAAAACTG TTGCTTTTGG CACCACATAC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

kif20a ( XM_018092219.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_017947708.1 Xenopus tropicalis kinesin family member 20A (kif20a), transcript variant X2, mRNA.

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CloneID	OXa07667
Clone ID Related Accession (Same CDS sequence)	XM_002936835.4 , XM_002936835.5 , XM_018092219.1
Accession Version	XM_018092219.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2733bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1473177600000
Organism	Xenopus tropicalis(tropical clawed frog)
Product	kinesin-like protein KIF20A isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030679.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTTAGCA ATGTTGTATC CCCTGGCATG GGACTGCTCT CAGATGATGA GGAGAACCCA 
GTGTTTGAAT CTACTGCAGC TGAATGTGGG GCAGGAATCC GAAAAGCACT TCTGTCTGAC 
TTCTCTGTCA TCTCACCAGG ACTGGGCCAT CCTCCCCAGT CTATGCCTGA GGAATCAAAT 
GACAAAGTCA TGGTTTATGT GAGGATTAGA CCCTTTGTAG ATGGGGAGTT GGAGAAAAAG 
GAGGACCAGG GCTGTGTTGA GGTGGAGAAT GAGGAGACCC TGGTGTTGCG TGCTCCTAAG 
GATTCCTTTG CAATGAAAAA TACAGAACGC GGACTGGGGC AGAGTGTGCA CAAATTTACT 
TTTTCTCAGC TATTTGGACC AGAAGTGGAC CAGAAGCAGT TTTTTGATGG AACTATGCGG 
CAAGTGGTAA AGGATGCTCT GAACGGCCAA AACTGGCTTA TTTATACTTA TGGCGTTACT 
AACTCTGGCA AAACCTTTAC CATACAAGGG ACTAGCAAGG ATAGGGGTAT TCTGCCTCGC 
TCTATTGCAC TTATCTTTAA CAGTGCCCAG GATCGCCTGT ATAAAAGCTC AGACCTCAAA 
CCTCTTTCTA ATGAGGTGAT GTGGCTGGAC AGCAAACAAG TGCGACAGGA AGAACTTAAA 
AAAGTTTCAC TGCTGTCTAA TCTGAGAGAG GAAGAACTTA TGACCCCCCT AAAAAGAAGT 
GGTAATGAAT CCCAGGTCCG GGCTGGCACA AGTGTTAGCT TTGACAGTGG CATTGGGGGA 
CTTTCTTCTA CAAGTCAGTT TGTCAATCAG ACTTGTAGCC AGCTAGAAGA GACCTGTTCA 
CGATGGAATG ACCAAGAAGT AGTATCGCTT CAGGAGGCTG CCGACGTACA ACTCTCCATC 
TGGGTATCCT ATTATGAAAT CTACAATGAA TTTGTTTATG ATTTACTGGA CTTCATGCCT 
TCTGGTCCGA ACCGTAAACG AACCACATTG CGACTGTGTG AGGACAGAAA TGGGAATCCA 
TATGTGAAAG ACCTCACCTG GATAAATGTA CACAGTGCTG AAGAAGCTTG GAAGATCCTA 
AGAGTTGGAA GGAAAAACCA GAGTTTTGCC AGTACACATC TGAACCAAAA CTCAAGCCGG 
AGTCACAGTA TCTTTTCCAT TCGGATTCTC CACATGCAAG GAAAAAATGA TTTGACGCCT 
AAAATAAGCG AGCTTTCTTT CTGTGACTTG GCTGGATCTG AGCGCTGTAA GGATCAGAGA 
AGCGGTGATC GCCTCAAGGA AGCAACTAAC ATTAATACCT CTTTGCACAC TCTGGGCCGC 
TGCATTACAG TATTGAGACA GAACCAACAA CAAAAGTTGC GACAGAACGT GGTCCCTTTC 
AGGGACAGTA AGCTGACCCG CATATTCCAG GCATACTTCA CTGGGCGTGG GAAGTCTTGC 
ATGATTGTAA ACATTAATCA GTGCGCTTCT ACATATGATG AGACTCTTTA TGCCATGAAG 
TTTTCAGCCA TTGCCAGCCA GTTGGTTCAA GCTCCACCAG TTAAAGTGAA CATTCCGTCC 
ATTCAGTCAC TCATCAAGGA AAGCACCATG CTAGCCAATC GCAGTTACAC AGATGAGGAG 
GATGCTGACG AAAATTCTGA TGAAGAAAGC GAGGAAACGG ATATTGCAAA ATTTAACAGA 
GAGGAGTTAT TACAAGTTAT TGAGAACATG AAGGAACTAA TTGTAAAGGA GCGTCAGGAG 
AAGCTGAACA TGGAGATACA AATCCGTAAG GAAGTTTGTA GCGAAATGAT GGATATGATG 
CAACAACAGC AGAAACAGCA TAGTGAGAGC CTGGAAGATG AGCGAGAATT GTTAGAGGAG 
ATATACGAGG GTCGGCTGGA GAACCTTAAA GAATCTCTAA CAAATTATTA CAAACACGAA 
TTGGAGGAAA GAGATGAGAG GATTGAAGAA CTGGAAGCTG CTTTGAAGGA AGCCATGGAA 
AGTGTCCATT CCATGCCAAG CCAGCCGACA CAAAGAGAAG AAAGTCTCTC GGTCAGAAGG 
TCTAAGCGAT TAGCAACAGC TGAGGCATCT AACACAGACA AATTTAAACA AGAGCTCCTG 
AATCTGAAAA TGGAGCTTCA GTCAAAGAAT GAGGAAATTG AAAAGTACAA GAAAATGCAA 
GAGCCTCCGG TCTCTGCCCG ATCATATACA GCTGATGTTG ATAAGAAGAT TGTGGAAGGG 
CAGAGACTTT ATGGTCTGGG CTCTCTTCAC AAGTGCACCG ATTTTCAGCA CACAAGAGCT 
CTTTGGAATG TAAGATTATT GCGCACTGAA TTGCAGAGGT TTGGGGATTC CTTGCAGCAG 
GTGGACAGGG TGTGCTGTCA TACAACTGGA GCTGAGAAAC TGAGGCAAGC TCTTGGTACT 
TGTGATGATA TACTGTCCAA ACAGGAAAGG GCTCTGGCAG AACTTCAAAA TAACATGGTG 
TTGGTGAAAA TGGACCTGAA GAAGAAGGCT GTTTGCATTG CTGAGCAGTA TAACACTGTA 
CAGAAACTAC AGGGAACCAC TTCTGGCTAC AAACGGCTCT GCAATAATGA GAATGTCCAA 
CCAAATAATC CAGAGAAGAA ACCCTTCCTA CGCAACTTCC TGGCAAGGAC CCCAGGAAAG 
AGAGGACTTG CAGAAAACAG TCCCTACAGT CGCATATTGC GCTCACGGCG ATCCCCTGTG 
CTTAAAACTG TTGCTTTTGG CACCACATAC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017947708.1
CDS	138..2870
Translation	MVSNVVSPGMGLLSDDEENPVFESTAAECGAGIRKALLSDFSVISPGLGHPPQSMPEESNDKVMVYVRIRPFVDGELEKKEDQGCVEVENEETLVLRAPKDSFAMKNTERGLGQSVHKFTFSQLFGPEVDQKQFFDGTMRQVVKDALNGQNWLIYTYGVTNSGKTFTIQGTSKDRGILPRSIALIFNSAQDRLYKSSDLKPLSNEVMWLDSKQVRQEELKKVSLLSNLREEELMTPLKRSGNESQVRAGTSVSFDSGIGGLSSTSQFVNQTCSQLEETCSRWNDQEVVSLQEAADVQLSIWVSYYEIYNEFVYDLLDFMPSGPNRKRTTLRLCEDRNGNPYVKDLTWINVHSAEEAWKILRVGRKNQSFASTHLNQNSSRSHSIFSIRILHMQGKNDLTPKISELSFCDLAGSERCKDQRSGDRLKEATNINTSLHTLGRCITVLRQNQQQKLRQNVVPFRDSKLTRIFQAYFTGRGKSCMIVNINQCASTYDETLYAMKFSAIASQLVQAPPVKVNIPSIQSLIKESTMLANRSYTDEEDADENSDEESEETDIAKFNREELLQVIENMKELIVKERQEKLNMEIQIRKEVCSEMMDMMQQQQKQHSESLEDERELLEEIYEGRLENLKESLTNYYKHELEERDERIEELEAALKEAMESVHSMPSQPTQREESLSVRRSKRLATAEASNTDKFKQELLNLKMELQSKNEEIEKYKKMQEPPVSARSYTADVDKKIVEGQRLYGLGSLHKCTDFQHTRALWNVRLLRTELQRFGDSLQQVDRVCCHTTGAEKLRQALGTCDDILSKQERALAELQNNMVLVKMDLKKKAVCIAEQYNTVQKLQGTTSGYKRLCNNENVQPNNPEKKPFLRNFLARTPGKRGLAENSPYSRILRSRRSPVLKTVAFGTTY

Target ORF information:

RefSeq Version	XM_018092219.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis kinesin family member 20A (kif20a), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_018092219.1

ORF Insert Sequence:

ATGGTTAGCA ATGTTGTATC CCCTGGCATG GGACTGCTCT CAGATGATGA GGAGAACCCA 
GTGTTTGAAT CTACTGCAGC TGAATGTGGG GCAGGAATCC GAAAAGCACT TCTGTCTGAC 
TTCTCTGTCA TCTCACCAGG ACTGGGCCAT CCTCCCCAGT CTATGCCTGA GGAATCAAAT 
GACAAAGTCA TGGTTTATGT GAGGATTAGA CCCTTTGTAG ATGGGGAGTT GGAGAAAAAG 
GAGGACCAGG GCTGTGTTGA GGTGGAGAAT GAGGAGACCC TGGTGTTGCG TGCTCCTAAG 
GATTCCTTTG CAATGAAAAA TACAGAACGC GGACTGGGGC AGAGTGTGCA CAAATTTACT 
TTTTCTCAGC TATTTGGACC AGAAGTGGAC CAGAAGCAGT TTTTTGATGG AACTATGCGG 
CAAGTGGTAA AGGATGCTCT GAACGGCCAA AACTGGCTTA TTTATACTTA TGGCGTTACT 
AACTCTGGCA AAACCTTTAC CATACAAGGG ACTAGCAAGG ATAGGGGTAT TCTGCCTCGC 
TCTATTGCAC TTATCTTTAA CAGTGCCCAG GATCGCCTGT ATAAAAGCTC AGACCTCAAA 
CCTCTTTCTA ATGAGGTGAT GTGGCTGGAC AGCAAACAAG TGCGACAGGA AGAACTTAAA 
AAAGTTTCAC TGCTGTCTAA TCTGAGAGAG GAAGAACTTA TGACCCCCCT AAAAAGAAGT 
GGTAATGAAT CCCAGGTCCG GGCTGGCACA AGTGTTAGCT TTGACAGTGG CATTGGGGGA 
CTTTCTTCTA CAAGTCAGTT TGTCAATCAG ACTTGTAGCC AGCTAGAAGA GACCTGTTCA 
CGATGGAATG ACCAAGAAGT AGTATCGCTT CAGGAGGCTG CCGACGTACA ACTCTCCATC 
TGGGTATCCT ATTATGAAAT CTACAATGAA TTTGTTTATG ATTTACTGGA CTTCATGCCT 
TCTGGTCCGA ACCGTAAACG AACCACATTG CGACTGTGTG AGGACAGAAA TGGGAATCCA 
TATGTGAAAG ACCTCACCTG GATAAATGTA CACAGTGCTG AAGAAGCTTG GAAGATCCTA 
AGAGTTGGAA GGAAAAACCA GAGTTTTGCC AGTACACATC TGAACCAAAA CTCAAGCCGG 
AGTCACAGTA TCTTTTCCAT TCGGATTCTC CACATGCAAG GAAAAAATGA TTTGACGCCT 
AAAATAAGCG AGCTTTCTTT CTGTGACTTG GCTGGATCTG AGCGCTGTAA GGATCAGAGA 
AGCGGTGATC GCCTCAAGGA AGCAACTAAC ATTAATACCT CTTTGCACAC TCTGGGCCGC 
TGCATTACAG TATTGAGACA GAACCAACAA CAAAAGTTGC GACAGAACGT GGTCCCTTTC 
AGGGACAGTA AGCTGACCCG CATATTCCAG GCATACTTCA CTGGGCGTGG GAAGTCTTGC 
ATGATTGTAA ACATTAATCA GTGCGCTTCT ACATATGATG AGACTCTTTA TGCCATGAAG 
TTTTCAGCCA TTGCCAGCCA GTTGGTTCAA GCTCCACCAG TTAAAGTGAA CATTCCGTCC 
ATTCAGTCAC TCATCAAGGA AAGCACCATG CTAGCCAATC GCAGTTACAC AGATGAGGAG 
GATGCTGACG AAAATTCTGA TGAAGAAAGC GAGGAAACGG ATATTGCAAA ATTTAACAGA 
GAGGAGTTAT TACAAGTTAT TGAGAACATG AAGGAACTAA TTGTAAAGGA GCGTCAGGAG 
AAGCTGAACA TGGAGATACA AATCCGTAAG GAAGTTTGTA GCGAAATGAT GGATATGATG 
CAACAACAGC AGAAACAGCA TAGTGAGAGC CTGGAAGATG AGCGAGAATT GTTAGAGGAG 
ATATACGAGG GTCGGCTGGA GAACCTTAAA GAATCTCTAA CAAATTATTA CAAACACGAA 
TTGGAGGAAA GAGATGAGAG GATTGAAGAA CTGGAAGCTG CTTTGAAGGA AGCCATGGAA 
AGTGTCCATT CCATGCCAAG CCAGCCGACA CAAAGAGAAG AAAGTCTCTC GGTCAGAAGG 
TCTAAGCGAT TAGCAACAGC TGAGGCATCT AACACAGACA AATTTAAACA AGAGCTCCTG 
AATCTGAAAA TGGAGCTTCA GTCAAAGAAT GAGGAAATTG AAAAGTACAA GAAAATGCAA 
GAGCCTCCGG TCTCTGCCCG ATCATATACA GCTGATGTTG ATAAGAAGAT TGTGGAAGGG 
CAGAGACTTT ATGGTCTGGG CTCTCTTCAC AAGTGCACCG ATTTTCAGCA CACAAGAGCT 
CTTTGGAATG TAAGATTATT GCGCACTGAA TTGCAGAGGT TTGGGGATTC CTTGCAGCAG 
GTGGACAGGG TGTGCTGTCA TACAACTGGA GCTGAGAAAC TGAGGCAAGC TCTTGGTACT 
TGTGATGATA TACTGTCCAA ACAGGAAAGG GCTCTGGCAG AACTTCAAAA TAACATGGTG 
TTGGTGAAAA TGGACCTGAA GAAGAAGGCT GTTTGCATTG CTGAGCAGTA TAACACTGTA 
CAGAAACTAC AGGGAACCAC TTCTGGCTAC AAACGGCTCT GCAATAATGA GAATGTCCAA 
CCAAATAATC CAGAGAAGAA ACCCTTCCTA CGCAACTTCC TGGCAAGGAC CCCAGGAAAG 
AGAGGACTTG CAGAAAACAG TCCCTACAGT CGCATATTGC GCTCACGGCG ATCCCCTGTG 
CTTAAAACTG TTGCTTTTGG CACCACATAC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

kif20a ( XM_004912879.3 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_004912936.1 Xenopus tropicalis kinesin family member 20A (kif20a), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OXa07668
Clone ID Related Accession (Same CDS sequence)	XM_004912879.3 , XM_004912879.4
Accession Version	XM_004912879.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2673bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1473177600000
Organism	Xenopus tropicalis(tropical clawed frog)
Product	kinesin-like protein KIF20A isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030679.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_004912879.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTTAGCA ATGTTGTATC CCCTGGCATG GGACTGCTCT CAGATGATGA GGAGAACCCA 
GTGTTTGAAT CTACTGCAGC TGAATGTGGG GCAGGAATCC GAAAAGCACT TCTGTCTGAC 
TTCTCTGTCA TCTCACCAGG ACTGGGCCAT CCTCCCCAGT CTATGCCTGA GGAATCAAAT 
GACAAAGTCA TGGTTTATGT GAGGATTAGA CCCTTTGTAG ATGGGGAGTT GGAGAAAAAG 
GAGGACCAGG GCTGTGTTGA GGTGGAGAAT GAGGAGACCC TGGTGTTGCG TGCTCCTAAG 
GATTCCTTTG CAATGAAAAA TACAGAACGC GGACTGGGGC AGAGTGTGCA CAAATTTACT 
TTTTCTCAGC TATTTGGACC AGAAGTGGAC CAGAAGCAGT TTTTTGATGG AACTATGCGG 
CAAGTGGTAA AGGATGCTCT GAACGGCCAA AACTGGCTTA TTTATACTTA TGGCGTTACT 
AACTCTGGCA AAACCTTTAC CATACAAGGG ACTAGCAAGG ATAGGGGTAT TCTGCCTCGC 
TCTATTGCAC TTATCTTTAA CAGTGCCCAG GATCGCCTGT ATAAAAGCTC AGACCTCAAA 
CCTCTTTCTA ATGAGGTGAT GTGGCTGGAC AGCAAACAAG TGCGACAGGA AGAACTTAAA 
AAAGTTTCAC TGCTGTCTAA TCTGAGAGAG GAAGAACTTA TGACCCCCCT AAAAAGAAGT 
GGTAATGAAT CCCAGGTCCG GGCTGGCACA AGTGTTAGCT TTGACAGTGG CATTGGGGGA 
CTTTCTTCTA CAAGTCAGTT TGTCAATCAG ACTTGTAGCC AGCTAGAAGA GACCTGTTCA 
CGATGGAATG ACCAAGAAGT AGTATCGCTT CAGGAGGCTG CCGACGTACA ACTCTCCATC 
TGGGTATCCT ATTATGAAAT CTACAATGAA TTTGTTTATG ATTTACTGGA CTTCATGCCT 
TCTGGTCCGA ACCGTAAACG AACCACATTG CGACTGTGTG AGGACAGAAA TGGGAATCCA 
TATGTGAAAG ACCTCACCTG GATAAATGTA CACAGTGCTG AAGAAGCTTG GAAGATCCTA 
AGAGTTGGAA GGAAAAACCA GAGTTTTGCC AGTACACATC TGAACCAAAA CTCAAGCCGG 
AGTCACAGTA TCTTTTCCAT TCGGATTCTC CACATGCAAG GAAAAAATGA TTTGACGCCT 
AAAATAAGCG AGCTTTCTTT CTGTGACTTG GCTGGATCTG AGCGCTGTAA GGATCAGAGA 
AGCGGTGATC GCCTCAAGGA AGCAACTAAC ATTAATACCT CTTTGCACAC TCTGGGCCGC 
TGCATTACAG TATTGAGACA GAACCAACAA CAAAAGTTGC GACAGAACGT GGTCCCTTTC 
AGGGACAGTA AGCTGACCCG CATATTCCAG GCATACTTCA CTGGGCGTGG GAAGTCTTGC 
ATGATTGTAA ACATTAATCA GTGCGCTTCT ACATATGATG AGACTCTTTA TGCCATGAAG 
TTTTCAGCCA TTGCCAGCCA GTTGGTTCAA GCTCCACCAG TTAAAGTGAA CATTCCGTCC 
ATTCAGTCAC TCATCAAGGA AAGCACCATG CTAGCCAATC GCAGTTACAC AGATGAGGAG 
GATGCTGACG AAAATTCTGA TGAAGAAAGC GAGGAAACGG ATATTGCAAA ATTTAACAGA 
GAGGAGTTAT TACAAGTTAT TGAGAACATG AAGGAACTAA TTGTAAAGGA GCGTCAGGAG 
AAGCTGAACA TGGAGATACA AATCCGTAAG GAAGTTTGTA GCGAAATGAT GGATATGATG 
CAACAACAGC AGAAACAGCA TAGTGAGAGC CTGGAAGATG AGCGAGAATT GTTAGAGGAG 
ATATACGAGG GTCGGCTGGA GAACCTTAAA GAATCTCTAA CAAATTATTA CAAACACGAA 
TTGGAGGAAA GAGATGAGAG GATTGAAGAA CTGGAAGCTG CTTTGAAGGA AGCCATGGAA 
AGTGTCCATT CCATGCCAAG CCAGCCGACA CAAAGAGAAG AAAGTCTCTC GGTCAGAAGG 
TCTAAGCGAT TAGCAACAGC TGAGGCATCT AACACAGACA AATTTAAACA AGAGCTCCTG 
AATCTGAAAA TGGAGCTTCA GTCAAAGAAT GAGGAAATTG AAAAGTACAA GAAAATGCAA 
GAGCCTCCGG TCTCTGCCCG ATCATATACA GCTGATGTTG ATAAGAAGAT TGTGGAAGGG 
CAGAGAAATG TAAGATTATT GCGCACTGAA TTGCAGAGGT TTGGGGATTC CTTGCAGCAG 
GTGGACAGGG TGTGCTGTCA TACAACTGGA GCTGAGAAAC TGAGGCAAGC TCTTGGTACT 
TGTGATGATA TACTGTCCAA ACAGGAAAGG GCTCTGGCAG AACTTCAAAA TAACATGGTG 
TTGGTGAAAA TGGACCTGAA GAAGAAGGCT GTTTGCATTG CTGAGCAGTA TAACACTGTA 
CAGAAACTAC AGGGAACCAC TTCTGGCTAC AAACGGCTCT GCAATAATGA GAATGTCCAA 
CCAAATAATC CAGAGAAGAA ACCCTTCCTA CGCAACTTCC TGGCAAGGAC CCCAGGAAAG 
AGAGGACTTG CAGAAAACAG TCCCTACAGT CGCATATTGC GCTCACGGCG ATCCCCTGTG 
CTTAAAACTG TTGCTTTTGG CACCACATAC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004912936.1
CDS	164..2836
Translation	MVSNVVSPGMGLLSDDEENPVFESTAAECGAGIRKALLSDFSVISPGLGHPPQSMPEESNDKVMVYVRIRPFVDGELEKKEDQGCVEVENEETLVLRAPKDSFAMKNTERGLGQSVHKFTFSQLFGPEVDQKQFFDGTMRQVVKDALNGQNWLIYTYGVTNSGKTFTIQGTSKDRGILPRSIALIFNSAQDRLYKSSDLKPLSNEVMWLDSKQVRQEELKKVSLLSNLREEELMTPLKRSGNESQVRAGTSVSFDSGIGGLSSTSQFVNQTCSQLEETCSRWNDQEVVSLQEAADVQLSIWVSYYEIYNEFVYDLLDFMPSGPNRKRTTLRLCEDRNGNPYVKDLTWINVHSAEEAWKILRVGRKNQSFASTHLNQNSSRSHSIFSIRILHMQGKNDLTPKISELSFCDLAGSERCKDQRSGDRLKEATNINTSLHTLGRCITVLRQNQQQKLRQNVVPFRDSKLTRIFQAYFTGRGKSCMIVNINQCASTYDETLYAMKFSAIASQLVQAPPVKVNIPSIQSLIKESTMLANRSYTDEEDADENSDEESEETDIAKFNREELLQVIENMKELIVKERQEKLNMEIQIRKEVCSEMMDMMQQQQKQHSESLEDERELLEEIYEGRLENLKESLTNYYKHELEERDERIEELEAALKEAMESVHSMPSQPTQREESLSVRRSKRLATAEASNTDKFKQELLNLKMELQSKNEEIEKYKKMQEPPVSARSYTADVDKKIVEGQRNVRLLRTELQRFGDSLQQVDRVCCHTTGAEKLRQALGTCDDILSKQERALAELQNNMVLVKMDLKKKAVCIAEQYNTVQKLQGTTSGYKRLCNNENVQPNNPEKKPFLRNFLARTPGKRGLAENSPYSRILRSRRSPVLKTVAFGTTY

Target ORF information:

RefSeq Version	XM_004912879.3
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis kinesin family member 20A (kif20a), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004912879.3

ORF Insert Sequence:

ATGGTTAGCA ATGTTGTATC CCCTGGCATG GGACTGCTCT CAGATGATGA GGAGAACCCA 
GTGTTTGAAT CTACTGCAGC TGAATGTGGG GCAGGAATCC GAAAAGCACT TCTGTCTGAC 
TTCTCTGTCA TCTCACCAGG ACTGGGCCAT CCTCCCCAGT CTATGCCTGA GGAATCAAAT 
GACAAAGTCA TGGTTTATGT GAGGATTAGA CCCTTTGTAG ATGGGGAGTT GGAGAAAAAG 
GAGGACCAGG GCTGTGTTGA GGTGGAGAAT GAGGAGACCC TGGTGTTGCG TGCTCCTAAG 
GATTCCTTTG CAATGAAAAA TACAGAACGC GGACTGGGGC AGAGTGTGCA CAAATTTACT 
TTTTCTCAGC TATTTGGACC AGAAGTGGAC CAGAAGCAGT TTTTTGATGG AACTATGCGG 
CAAGTGGTAA AGGATGCTCT GAACGGCCAA AACTGGCTTA TTTATACTTA TGGCGTTACT 
AACTCTGGCA AAACCTTTAC CATACAAGGG ACTAGCAAGG ATAGGGGTAT TCTGCCTCGC 
TCTATTGCAC TTATCTTTAA CAGTGCCCAG GATCGCCTGT ATAAAAGCTC AGACCTCAAA 
CCTCTTTCTA ATGAGGTGAT GTGGCTGGAC AGCAAACAAG TGCGACAGGA AGAACTTAAA 
AAAGTTTCAC TGCTGTCTAA TCTGAGAGAG GAAGAACTTA TGACCCCCCT AAAAAGAAGT 
GGTAATGAAT CCCAGGTCCG GGCTGGCACA AGTGTTAGCT TTGACAGTGG CATTGGGGGA 
CTTTCTTCTA CAAGTCAGTT TGTCAATCAG ACTTGTAGCC AGCTAGAAGA GACCTGTTCA 
CGATGGAATG ACCAAGAAGT AGTATCGCTT CAGGAGGCTG CCGACGTACA ACTCTCCATC 
TGGGTATCCT ATTATGAAAT CTACAATGAA TTTGTTTATG ATTTACTGGA CTTCATGCCT 
TCTGGTCCGA ACCGTAAACG AACCACATTG CGACTGTGTG AGGACAGAAA TGGGAATCCA 
TATGTGAAAG ACCTCACCTG GATAAATGTA CACAGTGCTG AAGAAGCTTG GAAGATCCTA 
AGAGTTGGAA GGAAAAACCA GAGTTTTGCC AGTACACATC TGAACCAAAA CTCAAGCCGG 
AGTCACAGTA TCTTTTCCAT TCGGATTCTC CACATGCAAG GAAAAAATGA TTTGACGCCT 
AAAATAAGCG AGCTTTCTTT CTGTGACTTG GCTGGATCTG AGCGCTGTAA GGATCAGAGA 
AGCGGTGATC GCCTCAAGGA AGCAACTAAC ATTAATACCT CTTTGCACAC TCTGGGCCGC 
TGCATTACAG TATTGAGACA GAACCAACAA CAAAAGTTGC GACAGAACGT GGTCCCTTTC 
AGGGACAGTA AGCTGACCCG CATATTCCAG GCATACTTCA CTGGGCGTGG GAAGTCTTGC 
ATGATTGTAA ACATTAATCA GTGCGCTTCT ACATATGATG AGACTCTTTA TGCCATGAAG 
TTTTCAGCCA TTGCCAGCCA GTTGGTTCAA GCTCCACCAG TTAAAGTGAA CATTCCGTCC 
ATTCAGTCAC TCATCAAGGA AAGCACCATG CTAGCCAATC GCAGTTACAC AGATGAGGAG 
GATGCTGACG AAAATTCTGA TGAAGAAAGC GAGGAAACGG ATATTGCAAA ATTTAACAGA 
GAGGAGTTAT TACAAGTTAT TGAGAACATG AAGGAACTAA TTGTAAAGGA GCGTCAGGAG 
AAGCTGAACA TGGAGATACA AATCCGTAAG GAAGTTTGTA GCGAAATGAT GGATATGATG 
CAACAACAGC AGAAACAGCA TAGTGAGAGC CTGGAAGATG AGCGAGAATT GTTAGAGGAG 
ATATACGAGG GTCGGCTGGA GAACCTTAAA GAATCTCTAA CAAATTATTA CAAACACGAA 
TTGGAGGAAA GAGATGAGAG GATTGAAGAA CTGGAAGCTG CTTTGAAGGA AGCCATGGAA 
AGTGTCCATT CCATGCCAAG CCAGCCGACA CAAAGAGAAG AAAGTCTCTC GGTCAGAAGG 
TCTAAGCGAT TAGCAACAGC TGAGGCATCT AACACAGACA AATTTAAACA AGAGCTCCTG 
AATCTGAAAA TGGAGCTTCA GTCAAAGAAT GAGGAAATTG AAAAGTACAA GAAAATGCAA 
GAGCCTCCGG TCTCTGCCCG ATCATATACA GCTGATGTTG ATAAGAAGAT TGTGGAAGGG 
CAGAGAAATG TAAGATTATT GCGCACTGAA TTGCAGAGGT TTGGGGATTC CTTGCAGCAG 
GTGGACAGGG TGTGCTGTCA TACAACTGGA GCTGAGAAAC TGAGGCAAGC TCTTGGTACT 
TGTGATGATA TACTGTCCAA ACAGGAAAGG GCTCTGGCAG AACTTCAAAA TAACATGGTG 
TTGGTGAAAA TGGACCTGAA GAAGAAGGCT GTTTGCATTG CTGAGCAGTA TAACACTGTA 
CAGAAACTAC AGGGAACCAC TTCTGGCTAC AAACGGCTCT GCAATAATGA GAATGTCCAA 
CCAAATAATC CAGAGAAGAA ACCCTTCCTA CGCAACTTCC TGGCAAGGAC CCCAGGAAAG 
AGAGGACTTG CAGAAAACAG TCCCTACAGT CGCATATTGC GCTCACGGCG ATCCCCTGTG 
CTTAAAACTG TTGCTTTTGG CACCACATAC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

kif20a ( XM_004912879.4 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_004912936.1 Xenopus tropicalis kinesin family member 20A (kif20a), transcript variant X2, mRNA.

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CloneID	OXa07668
Clone ID Related Accession (Same CDS sequence)	XM_004912879.3 , XM_004912879.4
Accession Version	XM_004912879.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2673bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1576512000000
Organism	Xenopus tropicalis(tropical clawed frog)
Product	kinesin-like protein KIF20A isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030679.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_004912879.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTTAGCA ATGTTGTATC CCCTGGCATG GGACTGCTCT CAGATGATGA GGAGAACCCA 
GTGTTTGAAT CTACTGCAGC TGAATGTGGG GCAGGAATCC GAAAAGCACT TCTGTCTGAC 
TTCTCTGTCA TCTCACCAGG ACTGGGCCAT CCTCCCCAGT CTATGCCTGA GGAATCAAAT 
GACAAAGTCA TGGTTTATGT GAGGATTAGA CCCTTTGTAG ATGGGGAGTT GGAGAAAAAG 
GAGGACCAGG GCTGTGTTGA GGTGGAGAAT GAGGAGACCC TGGTGTTGCG TGCTCCTAAG 
GATTCCTTTG CAATGAAAAA TACAGAACGC GGACTGGGGC AGAGTGTGCA CAAATTTACT 
TTTTCTCAGC TATTTGGACC AGAAGTGGAC CAGAAGCAGT TTTTTGATGG AACTATGCGG 
CAAGTGGTAA AGGATGCTCT GAACGGCCAA AACTGGCTTA TTTATACTTA TGGCGTTACT 
AACTCTGGCA AAACCTTTAC CATACAAGGG ACTAGCAAGG ATAGGGGTAT TCTGCCTCGC 
TCTATTGCAC TTATCTTTAA CAGTGCCCAG GATCGCCTGT ATAAAAGCTC AGACCTCAAA 
CCTCTTTCTA ATGAGGTGAT GTGGCTGGAC AGCAAACAAG TGCGACAGGA AGAACTTAAA 
AAAGTTTCAC TGCTGTCTAA TCTGAGAGAG GAAGAACTTA TGACCCCCCT AAAAAGAAGT 
GGTAATGAAT CCCAGGTCCG GGCTGGCACA AGTGTTAGCT TTGACAGTGG CATTGGGGGA 
CTTTCTTCTA CAAGTCAGTT TGTCAATCAG ACTTGTAGCC AGCTAGAAGA GACCTGTTCA 
CGATGGAATG ACCAAGAAGT AGTATCGCTT CAGGAGGCTG CCGACGTACA ACTCTCCATC 
TGGGTATCCT ATTATGAAAT CTACAATGAA TTTGTTTATG ATTTACTGGA CTTCATGCCT 
TCTGGTCCGA ACCGTAAACG AACCACATTG CGACTGTGTG AGGACAGAAA TGGGAATCCA 
TATGTGAAAG ACCTCACCTG GATAAATGTA CACAGTGCTG AAGAAGCTTG GAAGATCCTA 
AGAGTTGGAA GGAAAAACCA GAGTTTTGCC AGTACACATC TGAACCAAAA CTCAAGCCGG 
AGTCACAGTA TCTTTTCCAT TCGGATTCTC CACATGCAAG GAAAAAATGA TTTGACGCCT 
AAAATAAGCG AGCTTTCTTT CTGTGACTTG GCTGGATCTG AGCGCTGTAA GGATCAGAGA 
AGCGGTGATC GCCTCAAGGA AGCAACTAAC ATTAATACCT CTTTGCACAC TCTGGGCCGC 
TGCATTACAG TATTGAGACA GAACCAACAA CAAAAGTTGC GACAGAACGT GGTCCCTTTC 
AGGGACAGTA AGCTGACCCG CATATTCCAG GCATACTTCA CTGGGCGTGG GAAGTCTTGC 
ATGATTGTAA ACATTAATCA GTGCGCTTCT ACATATGATG AGACTCTTTA TGCCATGAAG 
TTTTCAGCCA TTGCCAGCCA GTTGGTTCAA GCTCCACCAG TTAAAGTGAA CATTCCGTCC 
ATTCAGTCAC TCATCAAGGA AAGCACCATG CTAGCCAATC GCAGTTACAC AGATGAGGAG 
GATGCTGACG AAAATTCTGA TGAAGAAAGC GAGGAAACGG ATATTGCAAA ATTTAACAGA 
GAGGAGTTAT TACAAGTTAT TGAGAACATG AAGGAACTAA TTGTAAAGGA GCGTCAGGAG 
AAGCTGAACA TGGAGATACA AATCCGTAAG GAAGTTTGTA GCGAAATGAT GGATATGATG 
CAACAACAGC AGAAACAGCA TAGTGAGAGC CTGGAAGATG AGCGAGAATT GTTAGAGGAG 
ATATACGAGG GTCGGCTGGA GAACCTTAAA GAATCTCTAA CAAATTATTA CAAACACGAA 
TTGGAGGAAA GAGATGAGAG GATTGAAGAA CTGGAAGCTG CTTTGAAGGA AGCCATGGAA 
AGTGTCCATT CCATGCCAAG CCAGCCGACA CAAAGAGAAG AAAGTCTCTC GGTCAGAAGG 
TCTAAGCGAT TAGCAACAGC TGAGGCATCT AACACAGACA AATTTAAACA AGAGCTCCTG 
AATCTGAAAA TGGAGCTTCA GTCAAAGAAT GAGGAAATTG AAAAGTACAA GAAAATGCAA 
GAGCCTCCGG TCTCTGCCCG ATCATATACA GCTGATGTTG ATAAGAAGAT TGTGGAAGGG 
CAGAGAAATG TAAGATTATT GCGCACTGAA TTGCAGAGGT TTGGGGATTC CTTGCAGCAG 
GTGGACAGGG TGTGCTGTCA TACAACTGGA GCTGAGAAAC TGAGGCAAGC TCTTGGTACT 
TGTGATGATA TACTGTCCAA ACAGGAAAGG GCTCTGGCAG AACTTCAAAA TAACATGGTG 
TTGGTGAAAA TGGACCTGAA GAAGAAGGCT GTTTGCATTG CTGAGCAGTA TAACACTGTA 
CAGAAACTAC AGGGAACCAC TTCTGGCTAC AAACGGCTCT GCAATAATGA GAATGTCCAA 
CCAAATAATC CAGAGAAGAA ACCCTTCCTA CGCAACTTCC TGGCAAGGAC CCCAGGAAAG 
AGAGGACTTG CAGAAAACAG TCCCTACAGT CGCATATTGC GCTCACGGCG ATCCCCTGTG 
CTTAAAACTG TTGCTTTTGG CACCACATAC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004912936.1
CDS	162..2834
Translation	MVSNVVSPGMGLLSDDEENPVFESTAAECGAGIRKALLSDFSVISPGLGHPPQSMPEESNDKVMVYVRIRPFVDGELEKKEDQGCVEVENEETLVLRAPKDSFAMKNTERGLGQSVHKFTFSQLFGPEVDQKQFFDGTMRQVVKDALNGQNWLIYTYGVTNSGKTFTIQGTSKDRGILPRSIALIFNSAQDRLYKSSDLKPLSNEVMWLDSKQVRQEELKKVSLLSNLREEELMTPLKRSGNESQVRAGTSVSFDSGIGGLSSTSQFVNQTCSQLEETCSRWNDQEVVSLQEAADVQLSIWVSYYEIYNEFVYDLLDFMPSGPNRKRTTLRLCEDRNGNPYVKDLTWINVHSAEEAWKILRVGRKNQSFASTHLNQNSSRSHSIFSIRILHMQGKNDLTPKISELSFCDLAGSERCKDQRSGDRLKEATNINTSLHTLGRCITVLRQNQQQKLRQNVVPFRDSKLTRIFQAYFTGRGKSCMIVNINQCASTYDETLYAMKFSAIASQLVQAPPVKVNIPSIQSLIKESTMLANRSYTDEEDADENSDEESEETDIAKFNREELLQVIENMKELIVKERQEKLNMEIQIRKEVCSEMMDMMQQQQKQHSESLEDERELLEEIYEGRLENLKESLTNYYKHELEERDERIEELEAALKEAMESVHSMPSQPTQREESLSVRRSKRLATAEASNTDKFKQELLNLKMELQSKNEEIEKYKKMQEPPVSARSYTADVDKKIVEGQRNVRLLRTELQRFGDSLQQVDRVCCHTTGAEKLRQALGTCDDILSKQERALAELQNNMVLVKMDLKKKAVCIAEQYNTVQKLQGTTSGYKRLCNNENVQPNNPEKKPFLRNFLARTPGKRGLAENSPYSRILRSRRSPVLKTVAFGTTY

Target ORF information:

RefSeq Version	XM_004912879.4
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis kinesin family member 20A (kif20a), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004912879.4

ORF Insert Sequence:

ATGGTTAGCA ATGTTGTATC CCCTGGCATG GGACTGCTCT CAGATGATGA GGAGAACCCA 
GTGTTTGAAT CTACTGCAGC TGAATGTGGG GCAGGAATCC GAAAAGCACT TCTGTCTGAC 
TTCTCTGTCA TCTCACCAGG ACTGGGCCAT CCTCCCCAGT CTATGCCTGA GGAATCAAAT 
GACAAAGTCA TGGTTTATGT GAGGATTAGA CCCTTTGTAG ATGGGGAGTT GGAGAAAAAG 
GAGGACCAGG GCTGTGTTGA GGTGGAGAAT GAGGAGACCC TGGTGTTGCG TGCTCCTAAG 
GATTCCTTTG CAATGAAAAA TACAGAACGC GGACTGGGGC AGAGTGTGCA CAAATTTACT 
TTTTCTCAGC TATTTGGACC AGAAGTGGAC CAGAAGCAGT TTTTTGATGG AACTATGCGG 
CAAGTGGTAA AGGATGCTCT GAACGGCCAA AACTGGCTTA TTTATACTTA TGGCGTTACT 
AACTCTGGCA AAACCTTTAC CATACAAGGG ACTAGCAAGG ATAGGGGTAT TCTGCCTCGC 
TCTATTGCAC TTATCTTTAA CAGTGCCCAG GATCGCCTGT ATAAAAGCTC AGACCTCAAA 
CCTCTTTCTA ATGAGGTGAT GTGGCTGGAC AGCAAACAAG TGCGACAGGA AGAACTTAAA 
AAAGTTTCAC TGCTGTCTAA TCTGAGAGAG GAAGAACTTA TGACCCCCCT AAAAAGAAGT 
GGTAATGAAT CCCAGGTCCG GGCTGGCACA AGTGTTAGCT TTGACAGTGG CATTGGGGGA 
CTTTCTTCTA CAAGTCAGTT TGTCAATCAG ACTTGTAGCC AGCTAGAAGA GACCTGTTCA 
CGATGGAATG ACCAAGAAGT AGTATCGCTT CAGGAGGCTG CCGACGTACA ACTCTCCATC 
TGGGTATCCT ATTATGAAAT CTACAATGAA TTTGTTTATG ATTTACTGGA CTTCATGCCT 
TCTGGTCCGA ACCGTAAACG AACCACATTG CGACTGTGTG AGGACAGAAA TGGGAATCCA 
TATGTGAAAG ACCTCACCTG GATAAATGTA CACAGTGCTG AAGAAGCTTG GAAGATCCTA 
AGAGTTGGAA GGAAAAACCA GAGTTTTGCC AGTACACATC TGAACCAAAA CTCAAGCCGG 
AGTCACAGTA TCTTTTCCAT TCGGATTCTC CACATGCAAG GAAAAAATGA TTTGACGCCT 
AAAATAAGCG AGCTTTCTTT CTGTGACTTG GCTGGATCTG AGCGCTGTAA GGATCAGAGA 
AGCGGTGATC GCCTCAAGGA AGCAACTAAC ATTAATACCT CTTTGCACAC TCTGGGCCGC 
TGCATTACAG TATTGAGACA GAACCAACAA CAAAAGTTGC GACAGAACGT GGTCCCTTTC 
AGGGACAGTA AGCTGACCCG CATATTCCAG GCATACTTCA CTGGGCGTGG GAAGTCTTGC 
ATGATTGTAA ACATTAATCA GTGCGCTTCT ACATATGATG AGACTCTTTA TGCCATGAAG 
TTTTCAGCCA TTGCCAGCCA GTTGGTTCAA GCTCCACCAG TTAAAGTGAA CATTCCGTCC 
ATTCAGTCAC TCATCAAGGA AAGCACCATG CTAGCCAATC GCAGTTACAC AGATGAGGAG 
GATGCTGACG AAAATTCTGA TGAAGAAAGC GAGGAAACGG ATATTGCAAA ATTTAACAGA 
GAGGAGTTAT TACAAGTTAT TGAGAACATG AAGGAACTAA TTGTAAAGGA GCGTCAGGAG 
AAGCTGAACA TGGAGATACA AATCCGTAAG GAAGTTTGTA GCGAAATGAT GGATATGATG 
CAACAACAGC AGAAACAGCA TAGTGAGAGC CTGGAAGATG AGCGAGAATT GTTAGAGGAG 
ATATACGAGG GTCGGCTGGA GAACCTTAAA GAATCTCTAA CAAATTATTA CAAACACGAA 
TTGGAGGAAA GAGATGAGAG GATTGAAGAA CTGGAAGCTG CTTTGAAGGA AGCCATGGAA 
AGTGTCCATT CCATGCCAAG CCAGCCGACA CAAAGAGAAG AAAGTCTCTC GGTCAGAAGG 
TCTAAGCGAT TAGCAACAGC TGAGGCATCT AACACAGACA AATTTAAACA AGAGCTCCTG 
AATCTGAAAA TGGAGCTTCA GTCAAAGAAT GAGGAAATTG AAAAGTACAA GAAAATGCAA 
GAGCCTCCGG TCTCTGCCCG ATCATATACA GCTGATGTTG ATAAGAAGAT TGTGGAAGGG 
CAGAGAAATG TAAGATTATT GCGCACTGAA TTGCAGAGGT TTGGGGATTC CTTGCAGCAG 
GTGGACAGGG TGTGCTGTCA TACAACTGGA GCTGAGAAAC TGAGGCAAGC TCTTGGTACT 
TGTGATGATA TACTGTCCAA ACAGGAAAGG GCTCTGGCAG AACTTCAAAA TAACATGGTG 
TTGGTGAAAA TGGACCTGAA GAAGAAGGCT GTTTGCATTG CTGAGCAGTA TAACACTGTA 
CAGAAACTAC AGGGAACCAC TTCTGGCTAC AAACGGCTCT GCAATAATGA GAATGTCCAA 
CCAAATAATC CAGAGAAGAA ACCCTTCCTA CGCAACTTCC TGGCAAGGAC CCCAGGAAAG 
AGAGGACTTG CAGAAAACAG TCCCTACAGT CGCATATTGC GCTCACGGCG ATCCCCTGTG 
CTTAAAACTG TTGCTTTTGG CACCACATAC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

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kif20a cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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