hira cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

Gene
Clones
gRNAs

Summary

Gene Symbol	hira
Entrez Gene ID	100495450
Full Name	histone cell cycle regulator
Synonyms	dgcr1,tup1,tuple1
General protein information	Preferred Names histone cell cycle regulator Names protein HIRA
Gene Type	protein-coding
Organism	Xenopus tropicalis(tropical clawed frog)

mRNA and Protein(s)

mRNA	Protein	Name
XM_002931904.4	XP_002931950.2	protein HIRA
XM_002931904.5	XP_002931950.2	protein HIRA

Pathways from BioSystems

Homologies

Homo sapiens (human)	HIRA	NP_003316.3
Mus musculus (house mouse)	Hira	NP_034565.2
Danio rerio (zebrafish)	hira	XP_696478.2
Bos taurus (cattle)	LOC101906870	XP_005218364.1
Macaca mulatta (Rhesus monkey)	HIRA	XP_001112873.2
Drosophila melanogaster (fruit fly)	Hira	NP_572401.2
Arabidopsis thaliana (thale cress)	HIRA	NP_001190009.1
Xenopus tropicalis (tropical clawed frog)	LOC100495450	XP_002931950.2
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP009488	XP_310209.4
Pan troglodytes (chimpanzee)	HIRA	XP_001165585.1
Canis lupus familiaris (dog)	HIRA	XP_543550.2
Rattus norvegicus (Norway rat)	Hira	NP_001129232.1
Oryza sativa (rice)	Os09g0567700	NP_001063964.1
Gallus gallus (chicken)	HIRA	XP_004945677.1
Caenorhabditis elegans (roundworm)	K10D2.1	NP_498101.2

Gene Ontology

The following hira gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the hira cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OXa03609	XM_002931904.5 Latest version!	Xenopus tropicalis histone cell cycle regulator (hira), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
View Old Accession Versions >>
OXa03609	XM_002931904.4	Xenopus tropicalis histone cell cycle regulator (hira), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

hira ( XM_002931904.4 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_002931950.2 Xenopus tropicalis histone cell cycle regulator (hira), mRNA.

Price & Availability↑

CloneID	OXa03609
Clone ID Related Accession (Same CDS sequence)	XM_002931904.4 , XM_002931904.5
Accession Version	XM_002931904.4	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3036bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1473177600000
Organism	Xenopus tropicalis(tropical clawed frog)
Product	protein HIRA
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_002931904.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGCTCC TGAAACCAAC CTGGGTTAAT CATAATGGCA AGCCCATTTT TTCAGTGGAC 
ATTCATCCAG ATGGCACCAA GTTTGCTACA GGAGGTCAAG GACAAGATTC TGGGAAAGTT 
GTTATCTGGA ATATGCCACC TTTACTCAAA GAGGAAGATG AAAAGAATGA AAATATTCCC 
AAGATGCTCT GCCAGATGGA CAACCACTTA GCCTGCGTGA ACTGCGTCCG GTGGTCCAAC 
AGTGGAGCTT ATTTAGCATC TGGAGGGGAT GACAAGCTTA TCATGGTGTG GAAACGATCA 
GGATACATTG GACCCAGCAC TGTTTTTGGT TCCAGCAGCA AATTGGCTAA TGTAGAGCAG 
TGGCGTTGCC TTTCCATTCT CAGGAGTCAT TCTGGAGATG TGATGGACGT ATCATGGTCA 
CCCCATGATG CCTGGTTAGC TTCATGCAGT GTGGATAACA CAGTGGTTAT ATGGAATGCC 
TTGAAATTCC CAGAGATCAT TTCCACATTA CGGGGCCACT CGGGGCTGGT GAAAGGACTA 
ACATGGGATC CTGTTGGCAA GTACATTGCT TCTCAGGCTG ATGACCGAAG CATCAAGGTC 
TGGAGGACGA TGGACTGGCA GCTAGAAACA AGCATCACAA AACCATTTGA TGAGTGTGGA 
GGAACAACCC ATGTCTTGCG GCTTAGCTGG TCTCCAGATG GACATTACCT GGTATCGGCT 
CATGCCATGA ACAACTCTGG CCCCACGGCC CAAATCATTG AACGTGATGG TTGGAAGGCA 
AACATGGACT TTGTGGGACA TCGTAAAGCT GTCACAGTGG TGAAATTCAA TCCAAAAATC 
TTTAAGAAGA AGCAAAAAAA TGGGAGCTCC ACAAAGACCA ACTGTCCTTA CTGCTGCTGT 
GCTGTTGGAA GCAAGGACCG GTCCTTGTCA GTATGGCTCA CTTGCCTTAA GAGGCCTTTG 
GTTGTAATAC ATGAACTTTT TGACAAATCC ATAATGGATA TCTCTTGGAC TTTAAATGGA 
CTGGGCATTT TGGTCTGTTC TATGGATGGA TCAGTTGCTT ATCTCGATTT CTCCCAGGAT 
GAGCTTGGTG ACCCATTAAG TGAGGAAGAG AAGAATAATA TCCACCAAAC TACATATGGA 
AAGAGTCTGG CCATCACAAC AGAGCCCCAG CTGTCTAACA CTGTTATAGA GAATCCAGAG 
ATGCTCAAGT TTCAGCAGAG GCAGCAGCCA CATCAGGATG GGGAACATAG GGTGCAAGCA 
CAGAGGGAGG CTCCAGCTCA TAATGTGGCA AGCATGGTGA ATGGGGAAAG TTTGGAGGAC 
ATAAGAAAGA ACCTCTTGAA AAAGCAAGTG GAGACACGGA CAGCAGATGG ACGGCGTAGG 
ATCACACCAC TCTGCATTGC TCAGCTAGAC ACTGGGGACT TTTCCACAGC ATTTTTTAAT 
AGTATTCCAA TATCTGGTGC ACTTCCGGGC TCAATGCTGA CTTCACAAAG CAACCAGCAA 
CTCATATCCT TGGATTCCAA CACAACCAGC TCTTCTGGGG CCCCCAAGTC TAATGCGGAA 
CTTGCAGGAA ACACAAAACC AGCTGAGGAA ACCGTAAATA AAGAGAGTGG GAATGCTTCA 
TCTTCCAGTC CTGTAGCCCC CAATAGCATC TCTGCCCAGC CAAAGATAGA ACCCATGAAG 
GCACTTGACT CCAGATTTAC CGAACGTTCA AAAGCCACCT CAGGAACAGC AGGAGTTGCT 
CATTTGAACC AAACAGCGGT TGACCGACTA AAAGAGCAAA ATGTTACAAA GGATTCAAAG 
CCACGAGAGG TCCTTGAGAG TAGCAGTGAC AGTGAGGAAA AGATTCCTGC AACCAAACAG 
CTTTCCAAAC GAAAAGGGGA GTCTGATGCT GATCTTGGAG AGAAGAGGAA GAAAGGGAGG 
CCACGCAAAG ACCCCCGACT GTCGGTCTCC CTCACTCTGC AGTCTCAGGT GGCATCAACC 
TCTGAAAAGG AATTAACATG TGTAACAACA TCCTCATTAA CCCTTAAGCT TCCAACTCCC 
ACTCCTCAAA AATCTTTTAC TGTACAGTTA AGCTCAGACC CTTCCATGTA CATTGAAGTT 
GAGAATGAAG TGAAAACAGT AGGTGGAAAC AAACTCAGCC AGCTCAAGTG TCACCGAGAA 
GGGAAAGAAT GGGAAGCGGT GCTCACAAGT CGTATCCTTG CAGCAGCAGG CAGCAACGAG 
GTGGTGTGCG TGGCCTGCGA GAAAAGAATG TTGTCTATTT TCTCAGGCAG TGGAAGGAGA 
CTGTTCCCAC CCATCATTCT TCCCTCACCT ATTTCCACGC TGCAGTGTAC AGGATCCTAT 
GTTATGGCTC TCACAGCTGC TGCTGCTCTC TCTGTCTGGG ATGTCCAGAA CCAGAAGGTA 
ATCATCAAAG ATGAATCATT ACAACCACTT TTATCAGGTA ACGATATGAC CGTTTCTCAG 
ACATTGCTAA CAAAGAGGGG GATACCGGTG CTAAACCTGT CCAATGGGAA AGCATATTGC 
TTCAGTCCCA GTCTCTCCAC ATGGAGTCTA GTCTCTGACA AACAGGATTC CTTGACACAG 
TGTGCAGATT ACCGGAACTG CATGCCCTCA CAAGACGCCA TCATGTGCTC TGGACCATTA 
GCCATAATTC AAGGACGAGC GTCTAATGCT GGCAGACAGG CTGCTCGCCT TTTTACGATG 
CCTCATCAAG TGCAGCAAGA AACCACCATG GCTTACCTTG AGAACCAGGT AGCAGCTGCT 
CTTATGCTGC AGTCCAGCCA GGAATACAGG CACTGGTTGC TCATCTATGC ACGATACCTG 
GTCAATGAAG GCTTTGAACA GAGGCTACGA GAAGTATGTC AGGACCTGCT AGGCCCAGTC 
CATAATTCTA GTGATAGTCA ATGGGAATCA AGAATTCTGG GTTTGCGGAA AAGAGAGCTA 
TTAAAGGAGC TTCTCCCTCT GATAGGACAG AACCTGCGTT TCCAGCGACT TTTCACAGAA 
TATCAAGAGC AGTTGGACAT TTTGAGGGAC AAATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_002931950.2
CDS	303..3338
Translation	MKLLKPTWVNHNGKPIFSVDIHPDGTKFATGGQGQDSGKVVIWNMPPLLKEEDEKNENIPKMLCQMDNHLACVNCVRWSNSGAYLASGGDDKLIMVWKRSGYIGPSTVFGSSSKLANVEQWRCLSILRSHSGDVMDVSWSPHDAWLASCSVDNTVVIWNALKFPEIISTLRGHSGLVKGLTWDPVGKYIASQADDRSIKVWRTMDWQLETSITKPFDECGGTTHVLRLSWSPDGHYLVSAHAMNNSGPTAQIIERDGWKANMDFVGHRKAVTVVKFNPKIFKKKQKNGSSTKTNCPYCCCAVGSKDRSLSVWLTCLKRPLVVIHELFDKSIMDISWTLNGLGILVCSMDGSVAYLDFSQDELGDPLSEEEKNNIHQTTYGKSLAITTEPQLSNTVIENPEMLKFQQRQQPHQDGEHRVQAQREAPAHNVASMVNGESLEDIRKNLLKKQVETRTADGRRRITPLCIAQLDTGDFSTAFFNSIPISGALPGSMLTSQSNQQLISLDSNTTSSSGAPKSNAELAGNTKPAEETVNKESGNASSSSPVAPNSISAQPKIEPMKALDSRFTERSKATSGTAGVAHLNQTAVDRLKEQNVTKDSKPREVLESSSDSEEKIPATKQLSKRKGESDADLGEKRKKGRPRKDPRLSVSLTLQSQVASTSEKELTCVTTSSLTLKLPTPTPQKSFTVQLSSDPSMYIEVENEVKTVGGNKLSQLKCHREGKEWEAVLTSRILAAAGSNEVVCVACEKRMLSIFSGSGRRLFPPIILPSPISTLQCTGSYVMALTAAAALSVWDVQNQKVIIKDESLQPLLSGNDMTVSQTLLTKRGIPVLNLSNGKAYCFSPSLSTWSLVSDKQDSLTQCADYRNCMPSQDAIMCSGPLAIIQGRASNAGRQAARLFTMPHQVQQETTMAYLENQVAAALMLQSSQEYRHWLLIYARYLVNEGFEQRLREVCQDLLGPVHNSSDSQWESRILGLRKRELLKELLPLIGQNLRFQRLFTEYQEQLDILRDK

Target ORF information:

RefSeq Version	XM_002931904.4
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis histone cell cycle regulator (hira), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_002931904.4

ORF Insert Sequence:

ATGAAGCTCC TGAAACCAAC CTGGGTTAAT CATAATGGCA AGCCCATTTT TTCAGTGGAC 
ATTCATCCAG ATGGCACCAA GTTTGCTACA GGAGGTCAAG GACAAGATTC TGGGAAAGTT 
GTTATCTGGA ATATGCCACC TTTACTCAAA GAGGAAGATG AAAAGAATGA AAATATTCCC 
AAGATGCTCT GCCAGATGGA CAACCACTTA GCCTGCGTGA ACTGCGTCCG GTGGTCCAAC 
AGTGGAGCTT ATTTAGCATC TGGAGGGGAT GACAAGCTTA TCATGGTGTG GAAACGATCA 
GGATACATTG GACCCAGCAC TGTTTTTGGT TCCAGCAGCA AATTGGCTAA TGTAGAGCAG 
TGGCGTTGCC TTTCCATTCT CAGGAGTCAT TCTGGAGATG TGATGGACGT ATCATGGTCA 
CCCCATGATG CCTGGTTAGC TTCATGCAGT GTGGATAACA CAGTGGTTAT ATGGAATGCC 
TTGAAATTCC CAGAGATCAT TTCCACATTA CGGGGCCACT CGGGGCTGGT GAAAGGACTA 
ACATGGGATC CTGTTGGCAA GTACATTGCT TCTCAGGCTG ATGACCGAAG CATCAAGGTC 
TGGAGGACGA TGGACTGGCA GCTAGAAACA AGCATCACAA AACCATTTGA TGAGTGTGGA 
GGAACAACCC ATGTCTTGCG GCTTAGCTGG TCTCCAGATG GACATTACCT GGTATCGGCT 
CATGCCATGA ACAACTCTGG CCCCACGGCC CAAATCATTG AACGTGATGG TTGGAAGGCA 
AACATGGACT TTGTGGGACA TCGTAAAGCT GTCACAGTGG TGAAATTCAA TCCAAAAATC 
TTTAAGAAGA AGCAAAAAAA TGGGAGCTCC ACAAAGACCA ACTGTCCTTA CTGCTGCTGT 
GCTGTTGGAA GCAAGGACCG GTCCTTGTCA GTATGGCTCA CTTGCCTTAA GAGGCCTTTG 
GTTGTAATAC ATGAACTTTT TGACAAATCC ATAATGGATA TCTCTTGGAC TTTAAATGGA 
CTGGGCATTT TGGTCTGTTC TATGGATGGA TCAGTTGCTT ATCTCGATTT CTCCCAGGAT 
GAGCTTGGTG ACCCATTAAG TGAGGAAGAG AAGAATAATA TCCACCAAAC TACATATGGA 
AAGAGTCTGG CCATCACAAC AGAGCCCCAG CTGTCTAACA CTGTTATAGA GAATCCAGAG 
ATGCTCAAGT TTCAGCAGAG GCAGCAGCCA CATCAGGATG GGGAACATAG GGTGCAAGCA 
CAGAGGGAGG CTCCAGCTCA TAATGTGGCA AGCATGGTGA ATGGGGAAAG TTTGGAGGAC 
ATAAGAAAGA ACCTCTTGAA AAAGCAAGTG GAGACACGGA CAGCAGATGG ACGGCGTAGG 
ATCACACCAC TCTGCATTGC TCAGCTAGAC ACTGGGGACT TTTCCACAGC ATTTTTTAAT 
AGTATTCCAA TATCTGGTGC ACTTCCGGGC TCAATGCTGA CTTCACAAAG CAACCAGCAA 
CTCATATCCT TGGATTCCAA CACAACCAGC TCTTCTGGGG CCCCCAAGTC TAATGCGGAA 
CTTGCAGGAA ACACAAAACC AGCTGAGGAA ACCGTAAATA AAGAGAGTGG GAATGCTTCA 
TCTTCCAGTC CTGTAGCCCC CAATAGCATC TCTGCCCAGC CAAAGATAGA ACCCATGAAG 
GCACTTGACT CCAGATTTAC CGAACGTTCA AAAGCCACCT CAGGAACAGC AGGAGTTGCT 
CATTTGAACC AAACAGCGGT TGACCGACTA AAAGAGCAAA ATGTTACAAA GGATTCAAAG 
CCACGAGAGG TCCTTGAGAG TAGCAGTGAC AGTGAGGAAA AGATTCCTGC AACCAAACAG 
CTTTCCAAAC GAAAAGGGGA GTCTGATGCT GATCTTGGAG AGAAGAGGAA GAAAGGGAGG 
CCACGCAAAG ACCCCCGACT GTCGGTCTCC CTCACTCTGC AGTCTCAGGT GGCATCAACC 
TCTGAAAAGG AATTAACATG TGTAACAACA TCCTCATTAA CCCTTAAGCT TCCAACTCCC 
ACTCCTCAAA AATCTTTTAC TGTACAGTTA AGCTCAGACC CTTCCATGTA CATTGAAGTT 
GAGAATGAAG TGAAAACAGT AGGTGGAAAC AAACTCAGCC AGCTCAAGTG TCACCGAGAA 
GGGAAAGAAT GGGAAGCGGT GCTCACAAGT CGTATCCTTG CAGCAGCAGG CAGCAACGAG 
GTGGTGTGCG TGGCCTGCGA GAAAAGAATG TTGTCTATTT TCTCAGGCAG TGGAAGGAGA 
CTGTTCCCAC CCATCATTCT TCCCTCACCT ATTTCCACGC TGCAGTGTAC AGGATCCTAT 
GTTATGGCTC TCACAGCTGC TGCTGCTCTC TCTGTCTGGG ATGTCCAGAA CCAGAAGGTA 
ATCATCAAAG ATGAATCATT ACAACCACTT TTATCAGGTA ACGATATGAC CGTTTCTCAG 
ACATTGCTAA CAAAGAGGGG GATACCGGTG CTAAACCTGT CCAATGGGAA AGCATATTGC 
TTCAGTCCCA GTCTCTCCAC ATGGAGTCTA GTCTCTGACA AACAGGATTC CTTGACACAG 
TGTGCAGATT ACCGGAACTG CATGCCCTCA CAAGACGCCA TCATGTGCTC TGGACCATTA 
GCCATAATTC AAGGACGAGC GTCTAATGCT GGCAGACAGG CTGCTCGCCT TTTTACGATG 
CCTCATCAAG TGCAGCAAGA AACCACCATG GCTTACCTTG AGAACCAGGT AGCAGCTGCT 
CTTATGCTGC AGTCCAGCCA GGAATACAGG CACTGGTTGC TCATCTATGC ACGATACCTG 
GTCAATGAAG GCTTTGAACA GAGGCTACGA GAAGTATGTC AGGACCTGCT AGGCCCAGTC 
CATAATTCTA GTGATAGTCA ATGGGAATCA AGAATTCTGG GTTTGCGGAA AAGAGAGCTA 
TTAAAGGAGC TTCTCCCTCT GATAGGACAG AACCTGCGTT TCCAGCGACT TTTCACAGAA 
TATCAAGAGC AGTTGGACAT TTTGAGGGAC AAATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

hira ( XM_002931904.5 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_002931950.2 Xenopus tropicalis histone cell cycle regulator (hira), mRNA.

Price & Availability↑

CloneID	OXa03609
Clone ID Related Accession (Same CDS sequence)	XM_002931904.4 , XM_002931904.5
Accession Version	XM_002931904.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3036bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1576512000000
Organism	Xenopus tropicalis(tropical clawed frog)
Product	protein HIRA
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_002931904.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGCTCC TGAAACCAAC CTGGGTTAAT CATAATGGCA AGCCCATTTT TTCAGTGGAC 
ATTCATCCAG ATGGCACCAA GTTTGCTACA GGAGGTCAAG GACAAGATTC TGGGAAAGTT 
GTTATCTGGA ATATGCCACC TTTACTCAAA GAGGAAGATG AAAAGAATGA AAATATTCCC 
AAGATGCTCT GCCAGATGGA CAACCACTTA GCCTGCGTGA ACTGCGTCCG GTGGTCCAAC 
AGTGGAGCTT ATTTAGCATC TGGAGGGGAT GACAAGCTTA TCATGGTGTG GAAACGATCA 
GGATACATTG GACCCAGCAC TGTTTTTGGT TCCAGCAGCA AATTGGCTAA TGTAGAGCAG 
TGGCGTTGCC TTTCCATTCT CAGGAGTCAT TCTGGAGATG TGATGGACGT ATCATGGTCA 
CCCCATGATG CCTGGTTAGC TTCATGCAGT GTGGATAACA CAGTGGTTAT ATGGAATGCC 
TTGAAATTCC CAGAGATCAT TTCCACATTA CGGGGCCACT CGGGGCTGGT GAAAGGACTA 
ACATGGGATC CTGTTGGCAA GTACATTGCT TCTCAGGCTG ATGACCGAAG CATCAAGGTC 
TGGAGGACGA TGGACTGGCA GCTAGAAACA AGCATCACAA AACCATTTGA TGAGTGTGGA 
GGAACAACCC ATGTCTTGCG GCTTAGCTGG TCTCCAGATG GACATTACCT GGTATCGGCT 
CATGCCATGA ACAACTCTGG CCCCACGGCC CAAATCATTG AACGTGATGG TTGGAAGGCA 
AACATGGACT TTGTGGGACA TCGTAAAGCT GTCACAGTGG TGAAATTCAA TCCAAAAATC 
TTTAAGAAGA AGCAAAAAAA TGGGAGCTCC ACAAAGACCA ACTGTCCTTA CTGCTGCTGT 
GCTGTTGGAA GCAAGGACCG GTCCTTGTCA GTATGGCTCA CTTGCCTTAA GAGGCCTTTG 
GTTGTAATAC ATGAACTTTT TGACAAATCC ATAATGGATA TCTCTTGGAC TTTAAATGGA 
CTGGGCATTT TGGTCTGTTC TATGGATGGA TCAGTTGCTT ATCTCGATTT CTCCCAGGAT 
GAGCTTGGTG ACCCATTAAG TGAGGAAGAG AAGAATAATA TCCACCAAAC TACATATGGA 
AAGAGTCTGG CCATCACAAC AGAGCCCCAG CTGTCTAACA CTGTTATAGA GAATCCAGAG 
ATGCTCAAGT TTCAGCAGAG GCAGCAGCCA CATCAGGATG GGGAACATAG GGTGCAAGCA 
CAGAGGGAGG CTCCAGCTCA TAATGTGGCA AGCATGGTGA ATGGGGAAAG TTTGGAGGAC 
ATAAGAAAGA ACCTCTTGAA AAAGCAAGTG GAGACACGGA CAGCAGATGG ACGGCGTAGG 
ATCACACCAC TCTGCATTGC TCAGCTAGAC ACTGGGGACT TTTCCACAGC ATTTTTTAAT 
AGTATTCCAA TATCTGGTGC ACTTCCGGGC TCAATGCTGA CTTCACAAAG CAACCAGCAA 
CTCATATCCT TGGATTCCAA CACAACCAGC TCTTCTGGGG CCCCCAAGTC TAATGCGGAA 
CTTGCAGGAA ACACAAAACC AGCTGAGGAA ACCGTAAATA AAGAGAGTGG GAATGCTTCA 
TCTTCCAGTC CTGTAGCCCC CAATAGCATC TCTGCCCAGC CAAAGATAGA ACCCATGAAG 
GCACTTGACT CCAGATTTAC CGAACGTTCA AAAGCCACCT CAGGAACAGC AGGAGTTGCT 
CATTTGAACC AAACAGCGGT TGACCGACTA AAAGAGCAAA ATGTTACAAA GGATTCAAAG 
CCACGAGAGG TCCTTGAGAG TAGCAGTGAC AGTGAGGAAA AGATTCCTGC AACCAAACAG 
CTTTCCAAAC GAAAAGGGGA GTCTGATGCT GATCTTGGAG AGAAGAGGAA GAAAGGGAGG 
CCACGCAAAG ACCCCCGACT GTCGGTCTCC CTCACTCTGC AGTCTCAGGT GGCATCAACC 
TCTGAAAAGG AATTAACATG TGTAACAACA TCCTCATTAA CCCTTAAGCT TCCAACTCCC 
ACTCCTCAAA AATCTTTTAC TGTACAGTTA AGCTCAGACC CTTCCATGTA CATTGAAGTT 
GAGAATGAAG TGAAAACAGT AGGTGGAAAC AAACTCAGCC AGCTCAAGTG TCACCGAGAA 
GGGAAAGAAT GGGAAGCGGT GCTCACAAGT CGTATCCTTG CAGCAGCAGG CAGCAACGAG 
GTGGTGTGCG TGGCCTGCGA GAAAAGAATG TTGTCTATTT TCTCAGGCAG TGGAAGGAGA 
CTGTTCCCAC CCATCATTCT TCCCTCACCT ATTTCCACGC TGCAGTGTAC AGGATCCTAT 
GTTATGGCTC TCACAGCTGC TGCTGCTCTC TCTGTCTGGG ATGTCCAGAA CCAGAAGGTA 
ATCATCAAAG ATGAATCATT ACAACCACTT TTATCAGGTA ACGATATGAC CGTTTCTCAG 
ACATTGCTAA CAAAGAGGGG GATACCGGTG CTAAACCTGT CCAATGGGAA AGCATATTGC 
TTCAGTCCCA GTCTCTCCAC ATGGAGTCTA GTCTCTGACA AACAGGATTC CTTGACACAG 
TGTGCAGATT ACCGGAACTG CATGCCCTCA CAAGACGCCA TCATGTGCTC TGGACCATTA 
GCCATAATTC AAGGACGAGC GTCTAATGCT GGCAGACAGG CTGCTCGCCT TTTTACGATG 
CCTCATCAAG TGCAGCAAGA AACCACCATG GCTTACCTTG AGAACCAGGT AGCAGCTGCT 
CTTATGCTGC AGTCCAGCCA GGAATACAGG CACTGGTTGC TCATCTATGC ACGATACCTG 
GTCAATGAAG GCTTTGAACA GAGGCTACGA GAAGTATGTC AGGACCTGCT AGGCCCAGTC 
CATAATTCTA GTGATAGTCA ATGGGAATCA AGAATTCTGG GTTTGCGGAA AAGAGAGCTA 
TTAAAGGAGC TTCTCCCTCT GATAGGACAG AACCTGCGTT TCCAGCGACT TTTCACAGAA 
TATCAAGAGC AGTTGGACAT TTTGAGGGAC AAATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_002931950.2
CDS	204..3239
Translation	MKLLKPTWVNHNGKPIFSVDIHPDGTKFATGGQGQDSGKVVIWNMPPLLKEEDEKNENIPKMLCQMDNHLACVNCVRWSNSGAYLASGGDDKLIMVWKRSGYIGPSTVFGSSSKLANVEQWRCLSILRSHSGDVMDVSWSPHDAWLASCSVDNTVVIWNALKFPEIISTLRGHSGLVKGLTWDPVGKYIASQADDRSIKVWRTMDWQLETSITKPFDECGGTTHVLRLSWSPDGHYLVSAHAMNNSGPTAQIIERDGWKANMDFVGHRKAVTVVKFNPKIFKKKQKNGSSTKTNCPYCCCAVGSKDRSLSVWLTCLKRPLVVIHELFDKSIMDISWTLNGLGILVCSMDGSVAYLDFSQDELGDPLSEEEKNNIHQTTYGKSLAITTEPQLSNTVIENPEMLKFQQRQQPHQDGEHRVQAQREAPAHNVASMVNGESLEDIRKNLLKKQVETRTADGRRRITPLCIAQLDTGDFSTAFFNSIPISGALPGSMLTSQSNQQLISLDSNTTSSSGAPKSNAELAGNTKPAEETVNKESGNASSSSPVAPNSISAQPKIEPMKALDSRFTERSKATSGTAGVAHLNQTAVDRLKEQNVTKDSKPREVLESSSDSEEKIPATKQLSKRKGESDADLGEKRKKGRPRKDPRLSVSLTLQSQVASTSEKELTCVTTSSLTLKLPTPTPQKSFTVQLSSDPSMYIEVENEVKTVGGNKLSQLKCHREGKEWEAVLTSRILAAAGSNEVVCVACEKRMLSIFSGSGRRLFPPIILPSPISTLQCTGSYVMALTAAAALSVWDVQNQKVIIKDESLQPLLSGNDMTVSQTLLTKRGIPVLNLSNGKAYCFSPSLSTWSLVSDKQDSLTQCADYRNCMPSQDAIMCSGPLAIIQGRASNAGRQAARLFTMPHQVQQETTMAYLENQVAAALMLQSSQEYRHWLLIYARYLVNEGFEQRLREVCQDLLGPVHNSSDSQWESRILGLRKRELLKELLPLIGQNLRFQRLFTEYQEQLDILRDK

Target ORF information:

RefSeq Version	XM_002931904.5
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis histone cell cycle regulator (hira), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_002931904.5

ORF Insert Sequence:

ATGAAGCTCC TGAAACCAAC CTGGGTTAAT CATAATGGCA AGCCCATTTT TTCAGTGGAC 
ATTCATCCAG ATGGCACCAA GTTTGCTACA GGAGGTCAAG GACAAGATTC TGGGAAAGTT 
GTTATCTGGA ATATGCCACC TTTACTCAAA GAGGAAGATG AAAAGAATGA AAATATTCCC 
AAGATGCTCT GCCAGATGGA CAACCACTTA GCCTGCGTGA ACTGCGTCCG GTGGTCCAAC 
AGTGGAGCTT ATTTAGCATC TGGAGGGGAT GACAAGCTTA TCATGGTGTG GAAACGATCA 
GGATACATTG GACCCAGCAC TGTTTTTGGT TCCAGCAGCA AATTGGCTAA TGTAGAGCAG 
TGGCGTTGCC TTTCCATTCT CAGGAGTCAT TCTGGAGATG TGATGGACGT ATCATGGTCA 
CCCCATGATG CCTGGTTAGC TTCATGCAGT GTGGATAACA CAGTGGTTAT ATGGAATGCC 
TTGAAATTCC CAGAGATCAT TTCCACATTA CGGGGCCACT CGGGGCTGGT GAAAGGACTA 
ACATGGGATC CTGTTGGCAA GTACATTGCT TCTCAGGCTG ATGACCGAAG CATCAAGGTC 
TGGAGGACGA TGGACTGGCA GCTAGAAACA AGCATCACAA AACCATTTGA TGAGTGTGGA 
GGAACAACCC ATGTCTTGCG GCTTAGCTGG TCTCCAGATG GACATTACCT GGTATCGGCT 
CATGCCATGA ACAACTCTGG CCCCACGGCC CAAATCATTG AACGTGATGG TTGGAAGGCA 
AACATGGACT TTGTGGGACA TCGTAAAGCT GTCACAGTGG TGAAATTCAA TCCAAAAATC 
TTTAAGAAGA AGCAAAAAAA TGGGAGCTCC ACAAAGACCA ACTGTCCTTA CTGCTGCTGT 
GCTGTTGGAA GCAAGGACCG GTCCTTGTCA GTATGGCTCA CTTGCCTTAA GAGGCCTTTG 
GTTGTAATAC ATGAACTTTT TGACAAATCC ATAATGGATA TCTCTTGGAC TTTAAATGGA 
CTGGGCATTT TGGTCTGTTC TATGGATGGA TCAGTTGCTT ATCTCGATTT CTCCCAGGAT 
GAGCTTGGTG ACCCATTAAG TGAGGAAGAG AAGAATAATA TCCACCAAAC TACATATGGA 
AAGAGTCTGG CCATCACAAC AGAGCCCCAG CTGTCTAACA CTGTTATAGA GAATCCAGAG 
ATGCTCAAGT TTCAGCAGAG GCAGCAGCCA CATCAGGATG GGGAACATAG GGTGCAAGCA 
CAGAGGGAGG CTCCAGCTCA TAATGTGGCA AGCATGGTGA ATGGGGAAAG TTTGGAGGAC 
ATAAGAAAGA ACCTCTTGAA AAAGCAAGTG GAGACACGGA CAGCAGATGG ACGGCGTAGG 
ATCACACCAC TCTGCATTGC TCAGCTAGAC ACTGGGGACT TTTCCACAGC ATTTTTTAAT 
AGTATTCCAA TATCTGGTGC ACTTCCGGGC TCAATGCTGA CTTCACAAAG CAACCAGCAA 
CTCATATCCT TGGATTCCAA CACAACCAGC TCTTCTGGGG CCCCCAAGTC TAATGCGGAA 
CTTGCAGGAA ACACAAAACC AGCTGAGGAA ACCGTAAATA AAGAGAGTGG GAATGCTTCA 
TCTTCCAGTC CTGTAGCCCC CAATAGCATC TCTGCCCAGC CAAAGATAGA ACCCATGAAG 
GCACTTGACT CCAGATTTAC CGAACGTTCA AAAGCCACCT CAGGAACAGC AGGAGTTGCT 
CATTTGAACC AAACAGCGGT TGACCGACTA AAAGAGCAAA ATGTTACAAA GGATTCAAAG 
CCACGAGAGG TCCTTGAGAG TAGCAGTGAC AGTGAGGAAA AGATTCCTGC AACCAAACAG 
CTTTCCAAAC GAAAAGGGGA GTCTGATGCT GATCTTGGAG AGAAGAGGAA GAAAGGGAGG 
CCACGCAAAG ACCCCCGACT GTCGGTCTCC CTCACTCTGC AGTCTCAGGT GGCATCAACC 
TCTGAAAAGG AATTAACATG TGTAACAACA TCCTCATTAA CCCTTAAGCT TCCAACTCCC 
ACTCCTCAAA AATCTTTTAC TGTACAGTTA AGCTCAGACC CTTCCATGTA CATTGAAGTT 
GAGAATGAAG TGAAAACAGT AGGTGGAAAC AAACTCAGCC AGCTCAAGTG TCACCGAGAA 
GGGAAAGAAT GGGAAGCGGT GCTCACAAGT CGTATCCTTG CAGCAGCAGG CAGCAACGAG 
GTGGTGTGCG TGGCCTGCGA GAAAAGAATG TTGTCTATTT TCTCAGGCAG TGGAAGGAGA 
CTGTTCCCAC CCATCATTCT TCCCTCACCT ATTTCCACGC TGCAGTGTAC AGGATCCTAT 
GTTATGGCTC TCACAGCTGC TGCTGCTCTC TCTGTCTGGG ATGTCCAGAA CCAGAAGGTA 
ATCATCAAAG ATGAATCATT ACAACCACTT TTATCAGGTA ACGATATGAC CGTTTCTCAG 
ACATTGCTAA CAAAGAGGGG GATACCGGTG CTAAACCTGT CCAATGGGAA AGCATATTGC 
TTCAGTCCCA GTCTCTCCAC ATGGAGTCTA GTCTCTGACA AACAGGATTC CTTGACACAG 
TGTGCAGATT ACCGGAACTG CATGCCCTCA CAAGACGCCA TCATGTGCTC TGGACCATTA 
GCCATAATTC AAGGACGAGC GTCTAATGCT GGCAGACAGG CTGCTCGCCT TTTTACGATG 
CCTCATCAAG TGCAGCAAGA AACCACCATG GCTTACCTTG AGAACCAGGT AGCAGCTGCT 
CTTATGCTGC AGTCCAGCCA GGAATACAGG CACTGGTTGC TCATCTATGC ACGATACCTG 
GTCAATGAAG GCTTTGAACA GAGGCTACGA GAAGTATGTC AGGACCTGCT AGGCCCAGTC 
CATAATTCTA GTGATAGTCA ATGGGAATCA AGAATTCTGG GTTTGCGGAA AAGAGAGCTA 
TTAAAGGAGC TTCTCCCTCT GATAGGACAG AACCTGCGTT TCCAGCGACT TTTCACAGAA 
TATCAAGAGC AGTTGGACAT TTTGAGGGAC AAATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

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hira cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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