itga1 cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

Gene
Clones
gRNAs

Summary

Gene Symbol	itga1
Entrez Gene ID	100492880
Full Name	integrin subunit alpha 1
Synonyms	cd49a
General protein information	Preferred Names integrin subunit alpha 1 Names integrin alpha-1 integrin, alpha 1
Gene Type	protein-coding
Organism	Xenopus tropicalis(tropical clawed frog)

mRNA and Protein(s)

mRNA	Protein	Name
XM_002933044.4	XP_002933090.3	integrin alpha-1
XM_002933044.3	XP_002933090.2	integrin alpha-1

Pathways from BioSystems

Homologies

Macaca mulatta (Rhesus monkey)	ITGA1	XP_001094788.1
Rattus norvegicus (Norway rat)	Itga1	NP_112256.1
Gallus gallus (chicken)	ITGA1	NP_990400.1
Pan troglodytes (chimpanzee)	ITGA1	XP_517769.2
Canis lupus familiaris (dog)	ITGA1	XP_005619392.1
Mus musculus (house mouse)	Itga1	NP_001028400.2
Danio rerio (zebrafish)	itga1	XP_699485.4
Xenopus tropicalis (tropical clawed frog)	itga1	XP_002933090.2
Homo sapiens (human)	ITGA1	NP_852478.1

Gene Ontology

The following itga1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the itga1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OXa03395	XM_002933044.3 Latest version!	Xenopus tropicalis integrin subunit alpha 1 (itga1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OXa49179	XM_002933044.4 Latest version!	Xenopus tropicalis integrin subunit alpha 1 (itga1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

itga1 ( XM_002933044.3 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_002933090.2 Xenopus tropicalis integrin subunit alpha 1 (itga1), mRNA.

Price & Availability↑

CloneID	OXa03395
Clone ID Related Accession (Same CDS sequence)	XM_002933044.3
Accession Version	XM_002933044.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3522bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1473177600000
Organism	Xenopus tropicalis(tropical clawed frog)
Product	integrin alpha-1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 23, 2015 this sequence version replaced XM_002933044.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGCTGTT TCTCTGCAGG GTGCTTGTAT CTCCTCACAG CCTTGCTGAA GCTTACGCAA 
TCATTTAATG TGGATGTAAA GAATGGACTT TCATTCAGTG GTTCGGCTGA AGATATGTTT 
GGATACACAG TTCAACAATT TGAAAATGAT GAGGGCAAAT GGGTACTTAT TGGGTCTCCT 
TTGGAAGGAC AACCAAAACA CAGAATGGGA GATGTTTACA AGTGTCCGGT TCACATGCCA 
TCCAATTGTT CAAAACTTAA CCTACCAAGA AATACAAGCA TCCCTGACAT TATTGAAGTA 
AAGGAAAATA TGGCCATGGG GTCAACTTTA GTGAAAAATC CTAAAGGTGG TTTTCTGGCA 
TGTGGTCCCT TATATGCTTA CAGATGTGGA AGTTTTCACT ATACAACTGG AATCTGCTCC 
AATGTCAGTT CTTCATTTGA AGTTTTAAAT GCCATTGCTC CATCAGTCCA AGGGTGCAGT 
AATCAGCTAG ATATTGTGAT AGTCCTTGAT GGTTCAAATA GTGTTTACCC ATGGGAGAGT 
GTCAATTATT TTTTACAGAA GCTTCTAAAA AATATGAACA TAAATCCTAA CCAGACTCAG 
GTTGGCATAG TTCAGTACGG GGAAAATGTA ACTCATGAAT TTAACCTGAA TACTTACGAT 
TCAACGGCCA AAGTGCGACA GGCATCCCTG GGAATTAAGC AAAGAGGTGG ATCTCAGACA 
ATGACAGCTC TTGGTATTGA CACAGCAAGA AAGGAAGCAT TTACCGAAGC AAGAGGGGCA 
CGAAAAGGAG TAAAAAAGGT TATGGTGGTT GTAACAGACG GGGAGTCACA TGACAATTAC 
AGACTAAACA GCGTTATCAA AGAGTGCGAG ACAGATGACA TTCAGAGATT TTCTATTGCA 
ATTCTGGGAA GCTACAACAG AGGGAATTTA AGTACAGAGA CCCTTGTAGA AGAGATAAAA 
TCCATTGCCA GTGAGCCTAA AGAGAAGCAT TTCTTTAATG TCTCGGATGA GTTGGCTCTT 
TTAAATATTG TGGAATCTCT GGGAGAAAGG ATATTCGCTC TTGAAGCTAC AAATGAAGAA 
CGGGCATCAT CTTTTCAAAT GGAAATGTCA CAGACTGGAT TGAGCGCTCA TTATTCAAAG 
GACTGGGCAA TGCTTGGCGC TGTTGGAGCA TATGACTGGA ATGGAACCGT GGTTATGGTG 
AATTCTGATG CGACTTTAAT ACCCAATAAC CAAACATTTA GAAAACGAAC AGATGAAAAG 
AATGAACCCC TGGCAGCTTA CCTTGGCTAC ACCTTGAGTT CTGCATCAGT ACATGGAGAA 
GAGATCTATA TTGCAGGACA ACCCCGATAC AACCATTCTG GCCAAGTGAT TATTTATAAA 
TTCAATGGAG AGGAAATTGA GGTACTGCAG ACATTAAATG GAGAACAGAT TGGATCATAT 
TTTGGCAGCG TGCTTACAAC AGTGGATGTG GATGGAGATT CAATTAGCGA CATACTTATC 
GTTGGAGCTC CAATGTATAT GGGAAGTGCC AAACAAGAAC AAGGAAAGGT GTACGTGTAT 
AGAATTCATG GGAAAGAAAT GCACTATCAG ATGAGTTTGG AGCCATTAAA CCAGACTTGC 
TGCTCTCAGC AGAAAGATAT CAGCTGCCAG AATGAGAACC AGAACGAGCC ATGTGGCTCT 
CGGTTTGGGA CGGCAATAGC AGCTGTAACG GATTTAAATT TGGATGGGTT TAATGATGTC 
GCTATAGGAG CCCCGTTAGA AGGGGATCAT CGGGGGGCTG TTTACATTTA TCACGGCAGT 
GGGAAATCTA TAAAAGAAAA CTATGCTCAG CGTATCCCAT CAGGGGGTGA TGGAAAGAAA 
GTCAGATTTT TTGGACAGTC TATTCATGGT GAAATGGACT TAAATGGAGA TGGTCTGACT 
GATGTCACTA TTGGAGGACT TGGGGGAGCA TCCCTTTTTT GGTCTCGGGA CGTTGCCGAA 
ATAACAGTAA CCACAGAGTT TACCCCAAAT GCAATTAATA TTGAAAAGAA GGACTGCACG 
GTCAGCAACA GACCTACCGT TTGCATTAAA GCAAGGTTTT GTTTTAATCT ACTGCTAAAG 
TCTGGTGAGG ACAAAAAAGC TAGTTCAGAT ATCATCTATA CCATCACATT GGACTCACCC 
CGATACGCCT CTCGAGTCTT ATTCAATGAT TCTCAGGATA GGAAGTTCCA AGGGGTTATG 
GGACACAAAG GGAATTGTCT GAATCATGAC TTTTATACGG TGGAAAAACC TGATTTCTTG 
AACTCCATTA ACGTCTCGGT TGAATTTAAT TTTCCTGACC CAGAAAAAGG CCCAGTTCTC 
AGCAGTGAAC TTCCAAACTT TTATAACTCA TACATTCCTT TCACAATGGA TTGTGGATCA 
GAACAGAAAT GTATTACTGA CTTAGTCCTA GATGCTGAAA TAAGTATGGA TGGAATCAGC 
TTTAATGAAG CAATTGTGAA GCCCACAAAG GACAAGTTCA ATCTGACTCT GACGGTTAAG 
AATATGAAGA AGAGTGCTTA TAATGCCAGG GTGATTGTCA AATATTCCCC AAACATTATA 
CTGGCTGCAA CTGAGGATAA GCAGAAAGTG AGCTGTGAAT CGGATCATGA TGTTACGTGC 
AAAATAGGAT ATCCTTTCCT GAAGAAGGAT GAAATGATTT CATTCAAAAT CACATTCCAG 
TTCAACTCCT CACATCTTTT GGATAACACC TTTATATTTG TCAATGCAAC AAGTGACAGT 
GAAGAGAAGC CAGAAACACT GATGAACAAT AGGAAGAACA TTACATTTAC GGTGAAATAT 
GAATCCTGGC TGACATTTGT AAGTTCTACA AAAGAGTTTC ATGTTCCGAT TGCAGCAAAC 
GACTCTATAC CGGCAACAAT CAATTCCACT GAAGCCATTG GGGCGGAAGT GAATATACAC 
TATGTGATTA GGAAGAATGA TTTTATTCCC ATGCCAAAGG TTTCATTCCG TCTTTCATTT 
CCCTACAAAA CACCAGACAA TAATATTCTC CTATATCTCA CCAACGTATC CTTCTCTCAC 
AATGTTAATT GCAGCTCTGA CCTATTGGAT CCACTGAGCA TAAATTCTGG AAAAACATAC 
ACAATGCCGG AGTTCAAAGA GAATTTAAGA GACACGATAT TGGACTGCAA AAGAAGTAAA 
TGCAGCTCAT TTGAGTGCAC AGTAGAGCCA GCCAATGATA CTCACATCAA CGTGTCACTA 
AGGGTCTGGA AATCAACTTT TATAAAGGCC AACATTCACA ATCTTAATCT TGTTCTGAAC 
GCAAAGCTGC AAAGTGAGAG CTCGTTGCTG GTCTTAAACC CCGGGAGTAG GAATTATGAG 
ACAATGATTA AAATATCAAA AGAATTGGAG CATGGAAAAG TGCCCTTCTG GGTTATTCCT 
CTTAGCATTA TCATTGGCCT ACTGATATTA GCCCTGATCA TATTTGCAAT GTGGAAGACT 
GGATTTTTCA AAAGGCCACT AAAAGAAAAG ATGGAAGAAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_002933090.2
CDS	522..4043
Translation	MGCFSAGCLYLLTALLKLTQSFNVDVKNGLSFSGSAEDMFGYTVQQFENDEGKWVLIGSPLEGQPKHRMGDVYKCPVHMPSNCSKLNLPRNTSIPDIIEVKENMAMGSTLVKNPKGGFLACGPLYAYRCGSFHYTTGICSNVSSSFEVLNAIAPSVQGCSNQLDIVIVLDGSNSVYPWESVNYFLQKLLKNMNINPNQTQVGIVQYGENVTHEFNLNTYDSTAKVRQASLGIKQRGGSQTMTALGIDTARKEAFTEARGARKGVKKVMVVVTDGESHDNYRLNSVIKECETDDIQRFSIAILGSYNRGNLSTETLVEEIKSIASEPKEKHFFNVSDELALLNIVESLGERIFALEATNEERASSFQMEMSQTGLSAHYSKDWAMLGAVGAYDWNGTVVMVNSDATLIPNNQTFRKRTDEKNEPLAAYLGYTLSSASVHGEEIYIAGQPRYNHSGQVIIYKFNGEEIEVLQTLNGEQIGSYFGSVLTTVDVDGDSISDILIVGAPMYMGSAKQEQGKVYVYRIHGKEMHYQMSLEPLNQTCCSQQKDISCQNENQNEPCGSRFGTAIAAVTDLNLDGFNDVAIGAPLEGDHRGAVYIYHGSGKSIKENYAQRIPSGGDGKKVRFFGQSIHGEMDLNGDGLTDVTIGGLGGASLFWSRDVAEITVTTEFTPNAINIEKKDCTVSNRPTVCIKARFCFNLLLKSGEDKKASSDIIYTITLDSPRYASRVLFNDSQDRKFQGVMGHKGNCLNHDFYTVEKPDFLNSINVSVEFNFPDPEKGPVLSSELPNFYNSYIPFTMDCGSEQKCITDLVLDAEISMDGISFNEAIVKPTKDKFNLTLTVKNMKKSAYNARVIVKYSPNIILAATEDKQKVSCESDHDVTCKIGYPFLKKDEMISFKITFQFNSSHLLDNTFIFVNATSDSEEKPETLMNNRKNITFTVKYESWLTFVSSTKEFHVPIAANDSIPATINSTEAIGAEVNIHYVIRKNDFIPMPKVSFRLSFPYKTPDNNILLYLTNVSFSHNVNCSSDLLDPLSINSGKTYTMPEFKENLRDTILDCKRSKCSSFECTVEPANDTHINVSLRVWKSTFIKANIHNLNLVLNAKLQSESSLLVLNPGSRNYETMIKISKELEHGKVPFWVIPLSIIIGLLILALIIFAMWKTGFFKRPLKEKMEE

Target ORF information:

RefSeq Version	XM_002933044.3
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis integrin subunit alpha 1 (itga1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_002933044.3

ORF Insert Sequence:

ATGGGCTGTT TCTCTGCAGG GTGCTTGTAT CTCCTCACAG CCTTGCTGAA GCTTACGCAA 
TCATTTAATG TGGATGTAAA GAATGGACTT TCATTCAGTG GTTCGGCTGA AGATATGTTT 
GGATACACAG TTCAACAATT TGAAAATGAT GAGGGCAAAT GGGTACTTAT TGGGTCTCCT 
TTGGAAGGAC AACCAAAACA CAGAATGGGA GATGTTTACA AGTGTCCGGT TCACATGCCA 
TCCAATTGTT CAAAACTTAA CCTACCAAGA AATACAAGCA TCCCTGACAT TATTGAAGTA 
AAGGAAAATA TGGCCATGGG GTCAACTTTA GTGAAAAATC CTAAAGGTGG TTTTCTGGCA 
TGTGGTCCCT TATATGCTTA CAGATGTGGA AGTTTTCACT ATACAACTGG AATCTGCTCC 
AATGTCAGTT CTTCATTTGA AGTTTTAAAT GCCATTGCTC CATCAGTCCA AGGGTGCAGT 
AATCAGCTAG ATATTGTGAT AGTCCTTGAT GGTTCAAATA GTGTTTACCC ATGGGAGAGT 
GTCAATTATT TTTTACAGAA GCTTCTAAAA AATATGAACA TAAATCCTAA CCAGACTCAG 
GTTGGCATAG TTCAGTACGG GGAAAATGTA ACTCATGAAT TTAACCTGAA TACTTACGAT 
TCAACGGCCA AAGTGCGACA GGCATCCCTG GGAATTAAGC AAAGAGGTGG ATCTCAGACA 
ATGACAGCTC TTGGTATTGA CACAGCAAGA AAGGAAGCAT TTACCGAAGC AAGAGGGGCA 
CGAAAAGGAG TAAAAAAGGT TATGGTGGTT GTAACAGACG GGGAGTCACA TGACAATTAC 
AGACTAAACA GCGTTATCAA AGAGTGCGAG ACAGATGACA TTCAGAGATT TTCTATTGCA 
ATTCTGGGAA GCTACAACAG AGGGAATTTA AGTACAGAGA CCCTTGTAGA AGAGATAAAA 
TCCATTGCCA GTGAGCCTAA AGAGAAGCAT TTCTTTAATG TCTCGGATGA GTTGGCTCTT 
TTAAATATTG TGGAATCTCT GGGAGAAAGG ATATTCGCTC TTGAAGCTAC AAATGAAGAA 
CGGGCATCAT CTTTTCAAAT GGAAATGTCA CAGACTGGAT TGAGCGCTCA TTATTCAAAG 
GACTGGGCAA TGCTTGGCGC TGTTGGAGCA TATGACTGGA ATGGAACCGT GGTTATGGTG 
AATTCTGATG CGACTTTAAT ACCCAATAAC CAAACATTTA GAAAACGAAC AGATGAAAAG 
AATGAACCCC TGGCAGCTTA CCTTGGCTAC ACCTTGAGTT CTGCATCAGT ACATGGAGAA 
GAGATCTATA TTGCAGGACA ACCCCGATAC AACCATTCTG GCCAAGTGAT TATTTATAAA 
TTCAATGGAG AGGAAATTGA GGTACTGCAG ACATTAAATG GAGAACAGAT TGGATCATAT 
TTTGGCAGCG TGCTTACAAC AGTGGATGTG GATGGAGATT CAATTAGCGA CATACTTATC 
GTTGGAGCTC CAATGTATAT GGGAAGTGCC AAACAAGAAC AAGGAAAGGT GTACGTGTAT 
AGAATTCATG GGAAAGAAAT GCACTATCAG ATGAGTTTGG AGCCATTAAA CCAGACTTGC 
TGCTCTCAGC AGAAAGATAT CAGCTGCCAG AATGAGAACC AGAACGAGCC ATGTGGCTCT 
CGGTTTGGGA CGGCAATAGC AGCTGTAACG GATTTAAATT TGGATGGGTT TAATGATGTC 
GCTATAGGAG CCCCGTTAGA AGGGGATCAT CGGGGGGCTG TTTACATTTA TCACGGCAGT 
GGGAAATCTA TAAAAGAAAA CTATGCTCAG CGTATCCCAT CAGGGGGTGA TGGAAAGAAA 
GTCAGATTTT TTGGACAGTC TATTCATGGT GAAATGGACT TAAATGGAGA TGGTCTGACT 
GATGTCACTA TTGGAGGACT TGGGGGAGCA TCCCTTTTTT GGTCTCGGGA CGTTGCCGAA 
ATAACAGTAA CCACAGAGTT TACCCCAAAT GCAATTAATA TTGAAAAGAA GGACTGCACG 
GTCAGCAACA GACCTACCGT TTGCATTAAA GCAAGGTTTT GTTTTAATCT ACTGCTAAAG 
TCTGGTGAGG ACAAAAAAGC TAGTTCAGAT ATCATCTATA CCATCACATT GGACTCACCC 
CGATACGCCT CTCGAGTCTT ATTCAATGAT TCTCAGGATA GGAAGTTCCA AGGGGTTATG 
GGACACAAAG GGAATTGTCT GAATCATGAC TTTTATACGG TGGAAAAACC TGATTTCTTG 
AACTCCATTA ACGTCTCGGT TGAATTTAAT TTTCCTGACC CAGAAAAAGG CCCAGTTCTC 
AGCAGTGAAC TTCCAAACTT TTATAACTCA TACATTCCTT TCACAATGGA TTGTGGATCA 
GAACAGAAAT GTATTACTGA CTTAGTCCTA GATGCTGAAA TAAGTATGGA TGGAATCAGC 
TTTAATGAAG CAATTGTGAA GCCCACAAAG GACAAGTTCA ATCTGACTCT GACGGTTAAG 
AATATGAAGA AGAGTGCTTA TAATGCCAGG GTGATTGTCA AATATTCCCC AAACATTATA 
CTGGCTGCAA CTGAGGATAA GCAGAAAGTG AGCTGTGAAT CGGATCATGA TGTTACGTGC 
AAAATAGGAT ATCCTTTCCT GAAGAAGGAT GAAATGATTT CATTCAAAAT CACATTCCAG 
TTCAACTCCT CACATCTTTT GGATAACACC TTTATATTTG TCAATGCAAC AAGTGACAGT 
GAAGAGAAGC CAGAAACACT GATGAACAAT AGGAAGAACA TTACATTTAC GGTGAAATAT 
GAATCCTGGC TGACATTTGT AAGTTCTACA AAAGAGTTTC ATGTTCCGAT TGCAGCAAAC 
GACTCTATAC CGGCAACAAT CAATTCCACT GAAGCCATTG GGGCGGAAGT GAATATACAC 
TATGTGATTA GGAAGAATGA TTTTATTCCC ATGCCAAAGG TTTCATTCCG TCTTTCATTT 
CCCTACAAAA CACCAGACAA TAATATTCTC CTATATCTCA CCAACGTATC CTTCTCTCAC 
AATGTTAATT GCAGCTCTGA CCTATTGGAT CCACTGAGCA TAAATTCTGG AAAAACATAC 
ACAATGCCGG AGTTCAAAGA GAATTTAAGA GACACGATAT TGGACTGCAA AAGAAGTAAA 
TGCAGCTCAT TTGAGTGCAC AGTAGAGCCA GCCAATGATA CTCACATCAA CGTGTCACTA 
AGGGTCTGGA AATCAACTTT TATAAAGGCC AACATTCACA ATCTTAATCT TGTTCTGAAC 
GCAAAGCTGC AAAGTGAGAG CTCGTTGCTG GTCTTAAACC CCGGGAGTAG GAATTATGAG 
ACAATGATTA AAATATCAAA AGAATTGGAG CATGGAAAAG TGCCCTTCTG GGTTATTCCT 
CTTAGCATTA TCATTGGCCT ACTGATATTA GCCCTGATCA TATTTGCAAT GTGGAAGACT 
GGATTTTTCA AAAGGCCACT AAAAGAAAAG ATGGAAGAAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

itga1 ( XM_002933044.4 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_002933090.3 Xenopus tropicalis integrin subunit alpha 1 (itga1), mRNA.

Price & Availability↑

CloneID	OXa49179
Clone ID Related Accession (Same CDS sequence)	XM_002933044.4
Accession Version	XM_002933044.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3522bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1576512000000
Organism	Xenopus tropicalis(tropical clawed frog)
Product	integrin alpha-1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_002933044.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGCTGTT TCTCTGCAGG GTGCTTGTAT CTCCTCACAG CCTTGCTGAA GCTTACGGAA 
TCATTTAATG TGGATGTAAA GAATGGACTT TCATTCAGTG GTTCGGCTGA AGATATGTTT 
GGATACACAG TTCAACAATT TGAAAATGAT GAGGGCAAAT GGGTACTTAT TGGGTCTCCT 
TTGGAAGGAC AACCAAAACA CAGAATGGGA GATGTTTACA AGTGTCCGGT TCACATGCCA 
TCCAATTGTT CAAAACTTAA CCTACCAAGA AATACAAGCA TCCCTGACAT TATTGAAGTA 
AAGGAAAATA TGGCCATGGG GTCAACTTTA GTGAAAAATC CTAAAGGCGG TTTTCTGGCA 
TGTGGTCCCT TATATGCTTA CAGATGTGGA AGTTTTCACT ATACAACTGG AATCTGCTCC 
AATGTCAGTT CTTCATTTGA AGTTTTAAAT GCCATTGCTC CATCAGTCCA AGGGTGCAGT 
AATCAGCTAG ATATTGTGAT AGTCCTTGAT GGTTCAAATA GTGTTTACCC ATGGGAGAGT 
GTCAATTATT TTTTACAGAA GCTTCTAAAA AATATGAACA TAAATCCTAA CCAGACTCAG 
GTTGGCATAG TTCAGTACGG GGAAAATGTA ACTCATGAAT TTAACCTGAA TACTTACGAT 
TCAACGGCCA AAGTGCGACA GGCATCCCTG GGAATTAAGC AAAGAGGTGG ATCTCAGACA 
ATGACAGCTC TTGGTATTGA CACAGCAAGA AAGGAAGCAT TTACCGAAGC AAGAGGGGCA 
CGAAAAGGAG TAAAAAAGGT TATGGTGGTT GTAACAGACG GGGAGTCACA TGACAATTAC 
AGACTAAACA GCGTTATCAA AGAGTGCGAG ACAGATGACA TTCAGAGATT TTCTATTGCA 
ATTCTGGGAA GCTACAACAG AGGGAATTTA AGTACAGAGA CCCTTGTAGA AGAGATAAAA 
TCCATTGCCA GTGAGCCTAA AGAGAAGCAT TTCTTTAATG TCTCGGATGA GTTGGCTCTT 
TTAAATATTG TGGAATCTCT GGGAGAAAGG ATATTCGCTC TTGAAGCTAC AAATGAAGAA 
CGGGCATCAT CTTTTCAAAT GGAAATGTCA CAGACTGGAT TGAGCGCTCA TTATTCAAAG 
GACTGGGCAA TGCTTGGCGC TGTTGGAGCA TATGACTGGA ATGGAACCGT GGTTATGGTG 
AATTCTGATG CGACTTTAAT ACCCAATAAC CAAACATTTA GAAAACGAAC AGATGAAAAG 
AATGAACCCC TGGCAGCTTA CCTTGGCTAC ACCTTGAGTT CTGCATCAGT ACATGGAGAA 
GAGATCTATA TTGCAGGACA ACCCCGATAC AACCATTCTG GCCAAGTGAT TATTTATAAA 
TTGAATGGAG AGGAAATTGA GGTACTGCAG ACATTAAATG GAGAACAGAT TGGATCATAT 
TTTGGCAGCG TGCTTACAAC AGTGGATGTG GATGGAGATT CAATTAGCGA CATACTTATC 
GTTGGAGCTC CAATGTATAT GGGAAGTGCC AAACAAGAAC AAGGAAAGGT GTACGTGTAT 
AGAATTCATG GGAAAGAAAT GCACTATCAG ATGAGTTTGG AGCCATTAAA CCAGACTTGC 
TGCTCTCAGC AGAAAGATAT CAGCTGCCAG AATGAGAACC AGAACGAGCC ATGTGGCTCT 
CGGTTTGGGA CGGCAATAGC AGCTGTAACG GATTTAAATT TGGATGGGTT TAATGATGTC 
GCTATAGGAG CCCCGTTAGA AGGGGATCAT CGGGGGGCTG TTTACATTTA TCACGGCAGT 
GGGAAATCTA TAAAAGAAAA CTATGCTCAG CGTATCCCAT CAGGGGGTGA TGGAAAGAAA 
GTCAGATTTT TTGGACAGTC TATTCATGGT GAAATGGATT TAAATGGAGA TGGTCTGACT 
GATGTCACTA TTGGAGGACT TGGGGGAGCA TCCCTTTTTT GGTCTCGGGA CGTTGCCGAA 
ATAACAGTAA CCACAGAGTT TACCCCAAAT GCAATTAATA TTGAAAAGAA GGACTGCACG 
GTCAGCAACA GACCTACCGT TTGCATTAAA GCAAGGTTTT GTTTTAATCT ACTGCTAAAG 
TCTGGTGAGG ACAAAAAAGC TAGTTCAGAT ATCATCTATA CCATCACATT GGACTCACCC 
CGATACGCCT CTCGAGTCTT ATTCAATGAT TCTCAGGATA GGAAGTTCCA AGGGGTTATG 
GGACACAAAG GGAATTGTCT GAATCATGAC TTTTATACGG TGGAAAAACC TGATTTCTTG 
AACTCCATTA ACGTCTCGGT TGAATTTAAT TTTCCTGACC CAGAAAAAGG CCCAGTTCTC 
AGCAGTGAAC TTCCAAACTT TTATAACTCA TACATTCCTT TCACAATGGA TTGTGGATCA 
GAACAGAAAT GTATTACTGA CTTAGTCCTA GATGCTGAAA TAAGTATGGA TGGAATCAGC 
TTTAATGAAG CAATTGTGAA GCCCACAAAG GACAAGTTCA ATCTGACTCT GACGGTTAAG 
AATATGAAGA AGAGTGCTTA TAATGCCAGG GTGATTGTCA AATATTCCCC AAACATTATA 
CTGGCTGCAA CTGAGGATAA GCAGAAAGTG AGCTGTGAAT CGGATCATGA TGTTACGTGC 
AAAATAGGAT ATCCTTTCCT GAAGAAGGAT GAAATGATTT CATTCAAAAT CACATTCCAG 
TTCAACTCCT CACATCTTTT GGATAACACC TTTATATTTG TCAATGCAAC AAGTGACAGT 
GAAGAGAAGC CAGAAACACT GATGAACAAT AGGAAGAACA TTACATTTAC GGTGAAATAT 
GAATCCTGGC TGACATTTGT AAGTTCTACA AAAGAGTTTC ATGTTCCGAT TGCAGCAAAC 
GACTCTATAC CGGCAACAAT CAATTCCACT GAAGCCATTG GGGCGGAAGT GAATATACAC 
TATGTGATTA GGAAGAATGA TTTTATTCCC ATGCCAAAGG TTTCATTCCG TCTTTCATTT 
CCCTACAAAA CACCAGACAA TAATATTCTC CTATATCTCA CCAACGTATC CTTCTCTCAC 
AATGTTAATT GCAGCTCTGA CCTATTGGAT CCACTGAGCA TAAATTCTGG AAAAACATAC 
ACAATGCCGG AGTTCAAAGA GAATTTAAGA GACACGATAT TGGACTGCAA AAGAAGTAAA 
TGCAGCTCAT TTGAGTGCAC AGTAGAGCCA GCCAATGATA CTCACATCAA CGTGTCACTA 
AGGGTCTGGA AATCAACTTT TATAAAGGCC AACATTCACA ATCTTAATCT TGTTCTGAAC 
GCAAAGCTGC AAAGTGAGAG CTCGTTGCTG GTCTTAAACC CCGGGAGTAG GAATTATGAG 
ACAATGATTA AAATATCAAA AGAATTGGAG CATGGAAAAG TGCCCTTCTG GGTTATTCCT 
CTTAGCATTA TCATTGGCCT ACTGATATTA GCCCTGATCA TATTTGCAAT GTGGAAGACT 
GGATTTTTCA AAAGGCCACT AAAAGAAAAG ATGGAAGAAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_002933090.3
CDS	526..4047
Translation	MGCFSAGCLYLLTALLKLTESFNVDVKNGLSFSGSAEDMFGYTVQQFENDEGKWVLIGSPLEGQPKHRMGDVYKCPVHMPSNCSKLNLPRNTSIPDIIEVKENMAMGSTLVKNPKGGFLACGPLYAYRCGSFHYTTGICSNVSSSFEVLNAIAPSVQGCSNQLDIVIVLDGSNSVYPWESVNYFLQKLLKNMNINPNQTQVGIVQYGENVTHEFNLNTYDSTAKVRQASLGIKQRGGSQTMTALGIDTARKEAFTEARGARKGVKKVMVVVTDGESHDNYRLNSVIKECETDDIQRFSIAILGSYNRGNLSTETLVEEIKSIASEPKEKHFFNVSDELALLNIVESLGERIFALEATNEERASSFQMEMSQTGLSAHYSKDWAMLGAVGAYDWNGTVVMVNSDATLIPNNQTFRKRTDEKNEPLAAYLGYTLSSASVHGEEIYIAGQPRYNHSGQVIIYKLNGEEIEVLQTLNGEQIGSYFGSVLTTVDVDGDSISDILIVGAPMYMGSAKQEQGKVYVYRIHGKEMHYQMSLEPLNQTCCSQQKDISCQNENQNEPCGSRFGTAIAAVTDLNLDGFNDVAIGAPLEGDHRGAVYIYHGSGKSIKENYAQRIPSGGDGKKVRFFGQSIHGEMDLNGDGLTDVTIGGLGGASLFWSRDVAEITVTTEFTPNAINIEKKDCTVSNRPTVCIKARFCFNLLLKSGEDKKASSDIIYTITLDSPRYASRVLFNDSQDRKFQGVMGHKGNCLNHDFYTVEKPDFLNSINVSVEFNFPDPEKGPVLSSELPNFYNSYIPFTMDCGSEQKCITDLVLDAEISMDGISFNEAIVKPTKDKFNLTLTVKNMKKSAYNARVIVKYSPNIILAATEDKQKVSCESDHDVTCKIGYPFLKKDEMISFKITFQFNSSHLLDNTFIFVNATSDSEEKPETLMNNRKNITFTVKYESWLTFVSSTKEFHVPIAANDSIPATINSTEAIGAEVNIHYVIRKNDFIPMPKVSFRLSFPYKTPDNNILLYLTNVSFSHNVNCSSDLLDPLSINSGKTYTMPEFKENLRDTILDCKRSKCSSFECTVEPANDTHINVSLRVWKSTFIKANIHNLNLVLNAKLQSESSLLVLNPGSRNYETMIKISKELEHGKVPFWVIPLSIIIGLLILALIIFAMWKTGFFKRPLKEKMEE

Target ORF information:

RefSeq Version	XM_002933044.4
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis integrin subunit alpha 1 (itga1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_002933044.4

ORF Insert Sequence:

ATGGGCTGTT TCTCTGCAGG GTGCTTGTAT CTCCTCACAG CCTTGCTGAA GCTTACGGAA 
TCATTTAATG TGGATGTAAA GAATGGACTT TCATTCAGTG GTTCGGCTGA AGATATGTTT 
GGATACACAG TTCAACAATT TGAAAATGAT GAGGGCAAAT GGGTACTTAT TGGGTCTCCT 
TTGGAAGGAC AACCAAAACA CAGAATGGGA GATGTTTACA AGTGTCCGGT TCACATGCCA 
TCCAATTGTT CAAAACTTAA CCTACCAAGA AATACAAGCA TCCCTGACAT TATTGAAGTA 
AAGGAAAATA TGGCCATGGG GTCAACTTTA GTGAAAAATC CTAAAGGCGG TTTTCTGGCA 
TGTGGTCCCT TATATGCTTA CAGATGTGGA AGTTTTCACT ATACAACTGG AATCTGCTCC 
AATGTCAGTT CTTCATTTGA AGTTTTAAAT GCCATTGCTC CATCAGTCCA AGGGTGCAGT 
AATCAGCTAG ATATTGTGAT AGTCCTTGAT GGTTCAAATA GTGTTTACCC ATGGGAGAGT 
GTCAATTATT TTTTACAGAA GCTTCTAAAA AATATGAACA TAAATCCTAA CCAGACTCAG 
GTTGGCATAG TTCAGTACGG GGAAAATGTA ACTCATGAAT TTAACCTGAA TACTTACGAT 
TCAACGGCCA AAGTGCGACA GGCATCCCTG GGAATTAAGC AAAGAGGTGG ATCTCAGACA 
ATGACAGCTC TTGGTATTGA CACAGCAAGA AAGGAAGCAT TTACCGAAGC AAGAGGGGCA 
CGAAAAGGAG TAAAAAAGGT TATGGTGGTT GTAACAGACG GGGAGTCACA TGACAATTAC 
AGACTAAACA GCGTTATCAA AGAGTGCGAG ACAGATGACA TTCAGAGATT TTCTATTGCA 
ATTCTGGGAA GCTACAACAG AGGGAATTTA AGTACAGAGA CCCTTGTAGA AGAGATAAAA 
TCCATTGCCA GTGAGCCTAA AGAGAAGCAT TTCTTTAATG TCTCGGATGA GTTGGCTCTT 
TTAAATATTG TGGAATCTCT GGGAGAAAGG ATATTCGCTC TTGAAGCTAC AAATGAAGAA 
CGGGCATCAT CTTTTCAAAT GGAAATGTCA CAGACTGGAT TGAGCGCTCA TTATTCAAAG 
GACTGGGCAA TGCTTGGCGC TGTTGGAGCA TATGACTGGA ATGGAACCGT GGTTATGGTG 
AATTCTGATG CGACTTTAAT ACCCAATAAC CAAACATTTA GAAAACGAAC AGATGAAAAG 
AATGAACCCC TGGCAGCTTA CCTTGGCTAC ACCTTGAGTT CTGCATCAGT ACATGGAGAA 
GAGATCTATA TTGCAGGACA ACCCCGATAC AACCATTCTG GCCAAGTGAT TATTTATAAA 
TTGAATGGAG AGGAAATTGA GGTACTGCAG ACATTAAATG GAGAACAGAT TGGATCATAT 
TTTGGCAGCG TGCTTACAAC AGTGGATGTG GATGGAGATT CAATTAGCGA CATACTTATC 
GTTGGAGCTC CAATGTATAT GGGAAGTGCC AAACAAGAAC AAGGAAAGGT GTACGTGTAT 
AGAATTCATG GGAAAGAAAT GCACTATCAG ATGAGTTTGG AGCCATTAAA CCAGACTTGC 
TGCTCTCAGC AGAAAGATAT CAGCTGCCAG AATGAGAACC AGAACGAGCC ATGTGGCTCT 
CGGTTTGGGA CGGCAATAGC AGCTGTAACG GATTTAAATT TGGATGGGTT TAATGATGTC 
GCTATAGGAG CCCCGTTAGA AGGGGATCAT CGGGGGGCTG TTTACATTTA TCACGGCAGT 
GGGAAATCTA TAAAAGAAAA CTATGCTCAG CGTATCCCAT CAGGGGGTGA TGGAAAGAAA 
GTCAGATTTT TTGGACAGTC TATTCATGGT GAAATGGATT TAAATGGAGA TGGTCTGACT 
GATGTCACTA TTGGAGGACT TGGGGGAGCA TCCCTTTTTT GGTCTCGGGA CGTTGCCGAA 
ATAACAGTAA CCACAGAGTT TACCCCAAAT GCAATTAATA TTGAAAAGAA GGACTGCACG 
GTCAGCAACA GACCTACCGT TTGCATTAAA GCAAGGTTTT GTTTTAATCT ACTGCTAAAG 
TCTGGTGAGG ACAAAAAAGC TAGTTCAGAT ATCATCTATA CCATCACATT GGACTCACCC 
CGATACGCCT CTCGAGTCTT ATTCAATGAT TCTCAGGATA GGAAGTTCCA AGGGGTTATG 
GGACACAAAG GGAATTGTCT GAATCATGAC TTTTATACGG TGGAAAAACC TGATTTCTTG 
AACTCCATTA ACGTCTCGGT TGAATTTAAT TTTCCTGACC CAGAAAAAGG CCCAGTTCTC 
AGCAGTGAAC TTCCAAACTT TTATAACTCA TACATTCCTT TCACAATGGA TTGTGGATCA 
GAACAGAAAT GTATTACTGA CTTAGTCCTA GATGCTGAAA TAAGTATGGA TGGAATCAGC 
TTTAATGAAG CAATTGTGAA GCCCACAAAG GACAAGTTCA ATCTGACTCT GACGGTTAAG 
AATATGAAGA AGAGTGCTTA TAATGCCAGG GTGATTGTCA AATATTCCCC AAACATTATA 
CTGGCTGCAA CTGAGGATAA GCAGAAAGTG AGCTGTGAAT CGGATCATGA TGTTACGTGC 
AAAATAGGAT ATCCTTTCCT GAAGAAGGAT GAAATGATTT CATTCAAAAT CACATTCCAG 
TTCAACTCCT CACATCTTTT GGATAACACC TTTATATTTG TCAATGCAAC AAGTGACAGT 
GAAGAGAAGC CAGAAACACT GATGAACAAT AGGAAGAACA TTACATTTAC GGTGAAATAT 
GAATCCTGGC TGACATTTGT AAGTTCTACA AAAGAGTTTC ATGTTCCGAT TGCAGCAAAC 
GACTCTATAC CGGCAACAAT CAATTCCACT GAAGCCATTG GGGCGGAAGT GAATATACAC 
TATGTGATTA GGAAGAATGA TTTTATTCCC ATGCCAAAGG TTTCATTCCG TCTTTCATTT 
CCCTACAAAA CACCAGACAA TAATATTCTC CTATATCTCA CCAACGTATC CTTCTCTCAC 
AATGTTAATT GCAGCTCTGA CCTATTGGAT CCACTGAGCA TAAATTCTGG AAAAACATAC 
ACAATGCCGG AGTTCAAAGA GAATTTAAGA GACACGATAT TGGACTGCAA AAGAAGTAAA 
TGCAGCTCAT TTGAGTGCAC AGTAGAGCCA GCCAATGATA CTCACATCAA CGTGTCACTA 
AGGGTCTGGA AATCAACTTT TATAAAGGCC AACATTCACA ATCTTAATCT TGTTCTGAAC 
GCAAAGCTGC AAAGTGAGAG CTCGTTGCTG GTCTTAAACC CCGGGAGTAG GAATTATGAG 
ACAATGATTA AAATATCAAA AGAATTGGAG CATGGAAAAG TGCCCTTCTG GGTTATTCCT 
CTTAGCATTA TCATTGGCCT ACTGATATTA GCCCTGATCA TATTTGCAAT GTGGAAGACT 
GGATTTTTCA AAAGGCCACT AAAAGAAAAG ATGGAAGAAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

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itga1 cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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