itga8 cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

Gene
Clones
gRNAs

Summary

Gene Symbol	itga8
Entrez Gene ID	100487624
Full Name	integrin subunit alpha 8
General protein information	Preferred Names integrin subunit alpha 8 Names integrin alpha-8 integrin, alpha 8
Gene Type	protein-coding
Organism	Xenopus tropicalis(tropical clawed frog)

mRNA and Protein(s)

mRNA	Protein	Name
XM_002939028.5	XP_002939074.2	integrin alpha-8
XM_002939028.4	XP_002939074.2	integrin alpha-8

Pathways from BioSystems

Homologies

Gallus gallus (chicken)	ITGA8	NP_990619.1
Caenorhabditis elegans (roundworm)	pat-2	NP_498948.1
Pan troglodytes (chimpanzee)	ITGA8	XP_507671.2
Bos taurus (cattle)	ITGA8	XP_002692127.1
Macaca mulatta (Rhesus monkey)	ITGA8	XP_001089317.1
Drosophila melanogaster (fruit fly)	if	NP_001162778.1
Canis lupus familiaris (dog)	ITGA8	XP_005617255.1
Mus musculus (house mouse)	Itga8	NP_001001309.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP000032	XP_311131.5
Homo sapiens (human)	ITGA8	NP_003629.1
Rattus norvegicus (Norway rat)	Itga8	NP_001167443.1
Xenopus tropicalis (tropical clawed frog)	itga8	XP_002939074.2

Gene Ontology

The following itga8 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the itga8 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OXa12011	XM_002939028.4 Latest version!	Xenopus tropicalis integrin subunit alpha 8 (itga8), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OXa60321	XM_002939028.5 Latest version!	Xenopus tropicalis integrin subunit alpha 8 (itga8), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

itga8 ( XM_002939028.4 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_002939074.2 Xenopus tropicalis integrin subunit alpha 8 (itga8), mRNA.

Price & Availability↑

CloneID	OXa12011
Clone ID Related Accession (Same CDS sequence)	XM_002939028.4
Accession Version	XM_002939028.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3246bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1473177600000
Organism	Xenopus tropicalis(tropical clawed frog)
Product	integrin alpha-8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030682.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_002939028.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGTTGC TCAGTAACAC ACACAGCGCT GCTGCCGCCT CACTGCTCCG GGCTCTCCTT 
CCGCGCCACC TTTACCCACA GAAGATGCTG AGCCGGTGGC GCCCTATCGC CCAGCAGCCA 
GCATGCCCCA TGCTCATGGT GATAATGCTG AGTGGCGTTT GTTCTGCCTT TAACCTGGAT 
GTAGAGAAAG TGACAGTGTA CAGCGGCCCC CCGGATAGTT ACTTTGGCTA CTCTGTGGAC 
TTCTACTTGC CCGACTCGGC AAAAACTAGT ATTCTCATTG GAGCGCCCAA AGCCAATACC 
TCTCAGCCAC AGATAGTGGA AGGAGGAGCC GTGTATTACT GTCCCTTCCC CGATACCCTG 
CCTACTGGAT GCAAGCAGAT TCCATTTGAT ATCACAAACA ACAGGAACAT CAGGTTAAAT 
GGAACCAAAC AGCCTGTTGA ATTCAAGTCA AACCAATGGT TTGGTGCAAC AGTCAGGGCT 
CATAAGGAAA AAGTTGTGGC TTGTGCCCCA CTGTATCACT GGAGAAGTAT TAAGCCCATT 
TCAGAAAAGG ATCCTGTTGG CACTTGCTAC ATTGCCATTC AGAATTTCAG TGCTTATGCT 
GAATACTCTC CCTGCCGAAG TGACCTTGCT GATCCTGCTG GACAAGGCGT CTGTCAAGCT 
GGCTTCAGTC TGGATTTTTA TAAGAATGGA GACCTGGCGT TGGGAGGGCC CGGAAGTTTT 
TACTGGCAAG GACAAGTCTT TACTGCCAGT ATAGCAGACA TAATTAAAGA CTATTCCTTC 
AAGAGCATTA TCAGGAAGAT ATCGGGCGAG AAACAAACAA AAATGGCACA ATCTGCTTAC 
GATGATAGCT ACTTAGGATA CTCTGTGGCT GTTGGAGAAT TTACTGATGA TTCAGTGCAA 
GAGGTGGTTG CCGGAGTTCC AAGGGGAGCA CAAAACTTTG GATATGTCTC TATCATCAAT 
TCAACTGACA TGACCTTCAT TCAGAACTTT ACAGGTGACC AGATGGCCTC TTACTTTGGA 
TACACTGTTG CTGTGTCAGA TGTAAACAAT GACGGGTTGG ATGATATCTT AGTTGGAGCC 
CCACTTTTCA TGGATCGAGA ATTCGAGAGC AATCCAAGGG AGGTTGGAAA AGTCTACTTG 
TACATGCAAG AAAGCGCTCT TGAATTTAGA GACCCCCAAG TACTGAAAGG CACAGAAGTC 
TTTGGGAGAT TTGGTAGTGC TATTGCCCAT TTAGGCGATC TTAATCAAGA TGGATACAAT 
GACATTGCAG TTGGCTCACC ATTTGCGGGG GAAGACAGAA AAGGCAGAGT GTTTATCTAC 
AATGGGAACA GCAATGGGTT AAACACAGAT ACCTCCCAGA TACTGGAAGG AGCTTGGGCC 
TCGCAGTCAA TGCCTGCTGG ATTTGGCTTT ACGCTGAGAG GCGATTCAGA CATAGACAAA 
AACGATTACC CAGATCTTAT TGTGGGAGCT TTTGGAGCAG GAAAAGCTGT TGTTTATAGA 
GCGAGACCAG TGATCACTGT TGATGCTCAA CTTTTGCTCC ACCCCATGAT TCTGAACCCA 
GAGAATAAAA CCTGCCAGCT CCCCAATTCA GAATTTCTGG TGACTTGTTT CACTGTAAGA 
GTGTGTGTGA CTGTGTCTGG GCAAAGCATC CCTTCAAATA TTGTTGTGAA AGCTGACCTT 
GAACTGGATT GGTTAAAACA GAAGGGTGCA GTGAAAAGAA CACTTTTTAT GGAATCACAC 
CAGGCCCACC ATTCTTTTGA TCTGATCATC AACAAACAAA GATTATCCCA GTGCTTGGAC 
TTCCTTGTTT ATCTCAGAGA AGAAAGTGAA TTCCGCGATA AGCTCACTCC AATCAGTGTC 
AACTTAAATT ACAGTTTGGA TGAATCGGCT TATCTGGGTG ACCAGGAACT GAAACCGATC 
CTCAATCGTT ACAAGAAAGC AACCATTCAA GAACAGGCGC ACATTCTAGT GGACTGCGGA 
GAAGACAACA TGTGCATTCC TGATCTCAAG CTCTCCGCCA GACCGGACAA GAATCAGCTT 
GTTATTGGAG AAGAGAACCA CATTCTGTTC ATCATAAACA CAAAAAACCA AGGGGAAGGA 
GCTTATGAAG CTGAGCTGCG CATGATTATC CCACCAGAAG CTGATTATGT TGGAATTGAG 
CGCAACATGG AGGGACTTCG GCAACTTAAC TGTGAATATA AAATGGAAAA TGAATCTCGA 
CTGGTGGTAT GCGATCTTGG CAACCCTATG GTGGCTGGAA CAAATCTGTC TGTCGGTCTG 
AGATTCGTTG TGCAACGATT AGAAACCGTA GATGCCATCA CATTTGATCT CTACATTAAA 
AGTTCCAACA AGGACAATCC AAACAGTAAC TTTGTCAGCT TGCAAGTTAA CATAACCGCT 
GTGGCCCAAG TAGAAATTAG AGGAGTGTCC CATCCTCCTC AGATCATTCT ACCCATTCCT 
AACTGGGAGC CGAAGGAGAA ACCATCTGAA GAAAAGGACG TTGGGCCGTT AGTGCAGCAT 
ATATATGAGC TGCACAACAT TGGCCCGAGT GCTATAAGTG AAACACAGCT GAATGTGGGA 
TGGCCTTCTC AAAGCCGAGA TGAGTTTCTC CTCTATGTAT TTCACATTCA GACTGAGGGT 
CCCCTGAAGT GCCAGGCAGG AAGTGCCATT AATACCTTAA ATATCAAACC AAGTGAACCT 
GAAGATACCC CTGAGCTTGC AGCTTTTCTG AGGAATACCA GTGTATCTCA TGCAGTAAGC 
CGCCGGGATG TAGCTGTGGC AGAGGCGCTG AGGCACATTC CCACACCGAC AAAACTACTG 
AATTGCACTA ATGTGAAATG TTTAATGATA TCCTGCAAAG TCGGCCACTT GGGTGGAGGT 
CAGCGAGCAG TGGTAAAAAT CCGGTCTCGT CTGTGGGCTC CAACATTCTT ACAGAGAAAA 
AATGATTATT ACTCACTTAT TGCCAATGTG TCATTTGAAG TCAAGAAGAT GCCTTATGAC 
ATTCAGCCAG CCAAGCTCCC AGCAGCAACT ATACTGATAA AAACATCTGT GATCTGGGCC 
ACACCAAATA TCTCATTTGC CATCCCTCTT TGGGTCATCT TATTGGCTAT ACTCCTTGGT 
ATACTGGTTC TTGCCATTCT GACACTGGTT ATGTGGAAGT GCGGGTTCTT CGACAGAGCG 
AGGCCCCCAC AGGATGACGT TGCAGATGAC ACTCAACAGT TAACAAACAA GACGGCAGAA 
GCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_002939074.2
CDS	199..3444
Translation	MKLLSNTHSAAAASLLRALLPRHLYPQKMLSRWRPIAQQPACPMLMVIMLSGVCSAFNLDVEKVTVYSGPPDSYFGYSVDFYLPDSAKTSILIGAPKANTSQPQIVEGGAVYYCPFPDTLPTGCKQIPFDITNNRNIRLNGTKQPVEFKSNQWFGATVRAHKEKVVACAPLYHWRSIKPISEKDPVGTCYIAIQNFSAYAEYSPCRSDLADPAGQGVCQAGFSLDFYKNGDLALGGPGSFYWQGQVFTASIADIIKDYSFKSIIRKISGEKQTKMAQSAYDDSYLGYSVAVGEFTDDSVQEVVAGVPRGAQNFGYVSIINSTDMTFIQNFTGDQMASYFGYTVAVSDVNNDGLDDILVGAPLFMDREFESNPREVGKVYLYMQESALEFRDPQVLKGTEVFGRFGSAIAHLGDLNQDGYNDIAVGSPFAGEDRKGRVFIYNGNSNGLNTDTSQILEGAWASQSMPAGFGFTLRGDSDIDKNDYPDLIVGAFGAGKAVVYRARPVITVDAQLLLHPMILNPENKTCQLPNSEFLVTCFTVRVCVTVSGQSIPSNIVVKADLELDWLKQKGAVKRTLFMESHQAHHSFDLIINKQRLSQCLDFLVYLREESEFRDKLTPISVNLNYSLDESAYLGDQELKPILNRYKKATIQEQAHILVDCGEDNMCIPDLKLSARPDKNQLVIGEENHILFIINTKNQGEGAYEAELRMIIPPEADYVGIERNMEGLRQLNCEYKMENESRLVVCDLGNPMVAGTNLSVGLRFVVQRLETVDAITFDLYIKSSNKDNPNSNFVSLQVNITAVAQVEIRGVSHPPQIILPIPNWEPKEKPSEEKDVGPLVQHIYELHNIGPSAISETQLNVGWPSQSRDEFLLYVFHIQTEGPLKCQAGSAINTLNIKPSEPEDTPELAAFLRNTSVSHAVSRRDVAVAEALRHIPTPTKLLNCTNVKCLMISCKVGHLGGGQRAVVKIRSRLWAPTFLQRKNDYYSLIANVSFEVKKMPYDIQPAKLPAATILIKTSVIWATPNISFAIPLWVILLAILLGILVLAILTLVMWKCGFFDRARPPQDDVADDTQQLTNKTAEA

Target ORF information:

RefSeq Version	XM_002939028.4
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis integrin subunit alpha 8 (itga8), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_002939028.4

ORF Insert Sequence:

ATGAAGTTGC TCAGTAACAC ACACAGCGCT GCTGCCGCCT CACTGCTCCG GGCTCTCCTT 
CCGCGCCACC TTTACCCACA GAAGATGCTG AGCCGGTGGC GCCCTATCGC CCAGCAGCCA 
GCATGCCCCA TGCTCATGGT GATAATGCTG AGTGGCGTTT GTTCTGCCTT TAACCTGGAT 
GTAGAGAAAG TGACAGTGTA CAGCGGCCCC CCGGATAGTT ACTTTGGCTA CTCTGTGGAC 
TTCTACTTGC CCGACTCGGC AAAAACTAGT ATTCTCATTG GAGCGCCCAA AGCCAATACC 
TCTCAGCCAC AGATAGTGGA AGGAGGAGCC GTGTATTACT GTCCCTTCCC CGATACCCTG 
CCTACTGGAT GCAAGCAGAT TCCATTTGAT ATCACAAACA ACAGGAACAT CAGGTTAAAT 
GGAACCAAAC AGCCTGTTGA ATTCAAGTCA AACCAATGGT TTGGTGCAAC AGTCAGGGCT 
CATAAGGAAA AAGTTGTGGC TTGTGCCCCA CTGTATCACT GGAGAAGTAT TAAGCCCATT 
TCAGAAAAGG ATCCTGTTGG CACTTGCTAC ATTGCCATTC AGAATTTCAG TGCTTATGCT 
GAATACTCTC CCTGCCGAAG TGACCTTGCT GATCCTGCTG GACAAGGCGT CTGTCAAGCT 
GGCTTCAGTC TGGATTTTTA TAAGAATGGA GACCTGGCGT TGGGAGGGCC CGGAAGTTTT 
TACTGGCAAG GACAAGTCTT TACTGCCAGT ATAGCAGACA TAATTAAAGA CTATTCCTTC 
AAGAGCATTA TCAGGAAGAT ATCGGGCGAG AAACAAACAA AAATGGCACA ATCTGCTTAC 
GATGATAGCT ACTTAGGATA CTCTGTGGCT GTTGGAGAAT TTACTGATGA TTCAGTGCAA 
GAGGTGGTTG CCGGAGTTCC AAGGGGAGCA CAAAACTTTG GATATGTCTC TATCATCAAT 
TCAACTGACA TGACCTTCAT TCAGAACTTT ACAGGTGACC AGATGGCCTC TTACTTTGGA 
TACACTGTTG CTGTGTCAGA TGTAAACAAT GACGGGTTGG ATGATATCTT AGTTGGAGCC 
CCACTTTTCA TGGATCGAGA ATTCGAGAGC AATCCAAGGG AGGTTGGAAA AGTCTACTTG 
TACATGCAAG AAAGCGCTCT TGAATTTAGA GACCCCCAAG TACTGAAAGG CACAGAAGTC 
TTTGGGAGAT TTGGTAGTGC TATTGCCCAT TTAGGCGATC TTAATCAAGA TGGATACAAT 
GACATTGCAG TTGGCTCACC ATTTGCGGGG GAAGACAGAA AAGGCAGAGT GTTTATCTAC 
AATGGGAACA GCAATGGGTT AAACACAGAT ACCTCCCAGA TACTGGAAGG AGCTTGGGCC 
TCGCAGTCAA TGCCTGCTGG ATTTGGCTTT ACGCTGAGAG GCGATTCAGA CATAGACAAA 
AACGATTACC CAGATCTTAT TGTGGGAGCT TTTGGAGCAG GAAAAGCTGT TGTTTATAGA 
GCGAGACCAG TGATCACTGT TGATGCTCAA CTTTTGCTCC ACCCCATGAT TCTGAACCCA 
GAGAATAAAA CCTGCCAGCT CCCCAATTCA GAATTTCTGG TGACTTGTTT CACTGTAAGA 
GTGTGTGTGA CTGTGTCTGG GCAAAGCATC CCTTCAAATA TTGTTGTGAA AGCTGACCTT 
GAACTGGATT GGTTAAAACA GAAGGGTGCA GTGAAAAGAA CACTTTTTAT GGAATCACAC 
CAGGCCCACC ATTCTTTTGA TCTGATCATC AACAAACAAA GATTATCCCA GTGCTTGGAC 
TTCCTTGTTT ATCTCAGAGA AGAAAGTGAA TTCCGCGATA AGCTCACTCC AATCAGTGTC 
AACTTAAATT ACAGTTTGGA TGAATCGGCT TATCTGGGTG ACCAGGAACT GAAACCGATC 
CTCAATCGTT ACAAGAAAGC AACCATTCAA GAACAGGCGC ACATTCTAGT GGACTGCGGA 
GAAGACAACA TGTGCATTCC TGATCTCAAG CTCTCCGCCA GACCGGACAA GAATCAGCTT 
GTTATTGGAG AAGAGAACCA CATTCTGTTC ATCATAAACA CAAAAAACCA AGGGGAAGGA 
GCTTATGAAG CTGAGCTGCG CATGATTATC CCACCAGAAG CTGATTATGT TGGAATTGAG 
CGCAACATGG AGGGACTTCG GCAACTTAAC TGTGAATATA AAATGGAAAA TGAATCTCGA 
CTGGTGGTAT GCGATCTTGG CAACCCTATG GTGGCTGGAA CAAATCTGTC TGTCGGTCTG 
AGATTCGTTG TGCAACGATT AGAAACCGTA GATGCCATCA CATTTGATCT CTACATTAAA 
AGTTCCAACA AGGACAATCC AAACAGTAAC TTTGTCAGCT TGCAAGTTAA CATAACCGCT 
GTGGCCCAAG TAGAAATTAG AGGAGTGTCC CATCCTCCTC AGATCATTCT ACCCATTCCT 
AACTGGGAGC CGAAGGAGAA ACCATCTGAA GAAAAGGACG TTGGGCCGTT AGTGCAGCAT 
ATATATGAGC TGCACAACAT TGGCCCGAGT GCTATAAGTG AAACACAGCT GAATGTGGGA 
TGGCCTTCTC AAAGCCGAGA TGAGTTTCTC CTCTATGTAT TTCACATTCA GACTGAGGGT 
CCCCTGAAGT GCCAGGCAGG AAGTGCCATT AATACCTTAA ATATCAAACC AAGTGAACCT 
GAAGATACCC CTGAGCTTGC AGCTTTTCTG AGGAATACCA GTGTATCTCA TGCAGTAAGC 
CGCCGGGATG TAGCTGTGGC AGAGGCGCTG AGGCACATTC CCACACCGAC AAAACTACTG 
AATTGCACTA ATGTGAAATG TTTAATGATA TCCTGCAAAG TCGGCCACTT GGGTGGAGGT 
CAGCGAGCAG TGGTAAAAAT CCGGTCTCGT CTGTGGGCTC CAACATTCTT ACAGAGAAAA 
AATGATTATT ACTCACTTAT TGCCAATGTG TCATTTGAAG TCAAGAAGAT GCCTTATGAC 
ATTCAGCCAG CCAAGCTCCC AGCAGCAACT ATACTGATAA AAACATCTGT GATCTGGGCC 
ACACCAAATA TCTCATTTGC CATCCCTCTT TGGGTCATCT TATTGGCTAT ACTCCTTGGT 
ATACTGGTTC TTGCCATTCT GACACTGGTT ATGTGGAAGT GCGGGTTCTT CGACAGAGCG 
AGGCCCCCAC AGGATGACGT TGCAGATGAC ACTCAACAGT TAACAAACAA GACGGCAGAA 
GCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

itga8 ( XM_002939028.5 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_002939074.2 Xenopus tropicalis integrin subunit alpha 8 (itga8), mRNA.

Price & Availability↑

CloneID	OXa60321
Clone ID Related Accession (Same CDS sequence)	XM_002939028.5
Accession Version	XM_002939028.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3246bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1576512000000
Organism	Xenopus tropicalis(tropical clawed frog)
Product	integrin alpha-8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030682.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_002939028.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGTTGC TCAGTAACAC ACACAGCGCT GCTGCCGCCT CACTGCTCCG GGCTCTCCTT 
CCGCGCCACC TTTACCCACA GAAGATGCTG AGCCGGTGGC GCCCTATCGC CCAGCAGCCA 
GCATGCCCCA TGCTCATGGT GATAATGCTG AGTGGCGTTT GTTCTGCCTT TAACCTGGAT 
GTAGAGAAAG TGACAGTGTA CAGCGGCCCC CCGGATAGTT ACTTTGGCTA CTCTGTGGAC 
TTCTACTTGC CCGACTCGGC AAAAACTAGT ATTCTCATTG GAGCGCCCAA AGCCAATACC 
TCTCAGCCAC AGATAGTGGA AGGAGGAGCC GTGTATTACT GTCCCTTCCC CGATACCCTG 
CCTACTGGAT GCAAGCAGAT TCCATTTGAT ATCACAAACA ACAGGAACAT CAGGTTAAAT 
GGAACCAAAC AGCCTGTTGA ATTCAAGTCA AACCAATGGT TTGGTGCAAC AGTCAGGGCT 
CATAAGGAAA AAGTTGTGGC TTGTGCCCCA CTGTATCACT GGAGAAGTAT TAAGCCCATT 
TCAGAAAAGG ATCCTGTTGG CACTTGCTAC ATTGCCATTC AGAATTTCAG TGCTTATGCT 
GAATACTCTC CCTGCCGAAG TGACCTTGCT GATCCTGCTG GACAAGGCGT CTGTCAAGCT 
GGCTTCAGTC TGGATTTTTA TAAGAATGGA GACCTGGCGT TGGGAGGGCC CGGAAGTTTT 
TACTGGCAAG GACAAGTCTT TACTGCCAGT ATAGCAGACA TAATTAAAGA CTATTCCTTC 
AAGAGCATTA TCAGGAAGAT ATCGGGCGAG AAACAAACAA AAATGGCACA ATCTGCTTAC 
GATGATAGCT ACTTAGGATA CTCTGTGGCT GTTGGAGAAT TTACTGATGA TTCAGTGCAA 
GAGGTGGTTG CCGGAGTTCC AAGGGGAGCA CAAAACTTTG GATATGTCTC TATCATCAAT 
TCAACTGACA TGACCTTCAT TCAGAACTTT ACAGGTGACC AGATGGCCTC TTACTTTGGA 
TACACTGTTG CTGTGTCAGA TGTAAACAAT GACGGGTTGG ATGATATCTT AGTTGGAGCC 
CCACTTTTCA TGGATCGAGA ATTCGAGAGC AATCCAAGGG AGGTTGGAAA AGTCTACTTG 
TACATGCAAG AAAGCGCTCT TGAATTTAGA GACCCCCAAG TACTGAAAGG CACAGAAGTC 
TTTGGGAGAT TTGGTAGTGC TATTGCCCAT TTAGGCGATC TTAATCAAGA TGGATACAAT 
GACATTGCAG TTGGCTCACC ATTTGCGGGG GAAGACAGAA AAGGCAGAGT GTTTATCTAC 
AATGGGAACA GCAATGGGTT AAACACAGAT ACCTCCCAGA TACTGGAAGG AGCTTGGGCC 
TCGCAGTCAA TGCCTGCTGG ATTTGGCTTT ACGCTGAGAG GCGATTCAGA CATAGACAAA 
AACGATTACC CAGATCTTAT TGTGGGAGCT TTTGGAGCAG GAAAAGCTGT TGTTTATAGA 
GCGAGACCAG TGATCACTGT TGATGCTCAA CTTTTGCTCC ACCCCATGAT TCTGAACCCA 
GAGAATAAAA CCTGCCAGCT CCCCAATTCA GAATTTCTGG TGACTTGTTT CACTGTAAGA 
GTGTGTGTGA CTGTGTCTGG GCAAAGCATC CCTTCAAATA TTGTTGTGAA AGCTGACCTT 
GAACTGGATT GGTTAAAACA GAAGGGTGCA GTGAAAAGAA CACTTTTTAT GGAATCACAC 
CAGGCCCACC ATTCTTTTGA TCTGATCATC AACAAACAAA GATTATCCCA GTGCTTGGAC 
TTCCTTGTTT ATCTCAGAGA AGAAAGTGAA TTCCGCGATA AGCTCACTCC AATCAGTGTC 
AACTTAAATT ACAGTTTGGA TGAATCGGCT TATCTGGGTG ACCAGGAACT GAAACCGATC 
CTCAATCGTT ACAAGAAAGC AACCATTCAA GAACAGGCGC ACATTCTAGT GGACTGCGGA 
GAAGACAACA TGTGCATTCC TGATCTCAAG CTCTCCGCCA GACCGGACAA GAATCAGCTT 
GTTATTGGAG AAGAGAACCA CATTCTGTTC ATCATAAACA CAAAAAACCA AGGGGAAGGA 
GCTTATGAAG CTGAGCTGCG CATGATTATC CCACCAGAAG CTGATTATGT TGGAATTGAG 
CGCAACATGG AGGGACTTCG GCAACTTAAC TGTGAATATA AAATGGAAAA TGAATCTCGA 
CTGGTGGTAT GCGATCTTGG CAACCCTATG GTGGCTGGAA CAAATCTGTC TGTCGGTCTG 
AGATTCGTTG TGCAACGATT AGAAACCGTA GATGCCATCA CATTTGATCT CTACATTAAA 
AGTTCCAACA AGGACAATCC AAACAGTAAC TTTGTCAGCT TGCAAGTTAA CATAACCGCT 
GTGGCCCAAG TAGAAATTAG AGGAGTGTCC CATCCTCCTC AGATCATTCT ACCCATTCCT 
AACTGGGAGC CGAAGGAGAA ACCATCTGAA GAAAAGGACG TTGGGCCGTT AGTGCAGCAT 
ATATATGAGC TGCACAACAT TGGCCCGAGT GCTATAAGTG AAACACAGCT GAATGTGGGA 
TGGCCTTCTC AAAGCCGAGA TGAGTTTCTC CTCTATGTAT TTCACATTCA GACTGAGGGT 
CCCCTGAAGT GCCAGGCAGG AAGTGCCATT AACACCTTAA ATATCAAACC AAGTGAACCT 
GAAGATACCC CTGAGCTTGC AGCTTTTCTG AGGAATACCA GTGTATCTCA TGCAGTAAGC 
CGCCGGGATG TAGCTGTGGC AGAGGCGCTG AGGCACATTC CCACACCGAC AAAACTACTG 
AATTGCACTA ATGTGAAATG TTTAATGATA TCCTGCAAAG TCGGCCACTT GGGTGGAGGT 
CAGCGAGCAG TGGTAAAAAT CCGGTCTCGT CTGTGGGCTC CAACATTCTT ACAGAGAAAA 
AATGATTATT ACTCACTTAT TGCCAATGTG TCATTTGAAG TCAAGAAGAT GCCTTATGAC 
ATTCAGCCAG CCAAGCTCCC AGCAGCAACT ATACTGATAA AAACATCTGT GATCTGGGCC 
ACACCAAATA TCTCATTTGC CATCCCTCTT TGGGTCATCT TATTGGCTAT ACTCCTTGGT 
ATACTGGTTC TTGCCATTCT GACACTGGTT ATGTGGAAGT GCGGGTTCTT CGACAGAGCG 
AGGCCCCCAC AGGATGACGT TGCAGATGAC ACTCAACAGT TAACAAACAA GACGGCAGAA 
GCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_002939074.2
CDS	205..3450
Translation	MKLLSNTHSAAAASLLRALLPRHLYPQKMLSRWRPIAQQPACPMLMVIMLSGVCSAFNLDVEKVTVYSGPPDSYFGYSVDFYLPDSAKTSILIGAPKANTSQPQIVEGGAVYYCPFPDTLPTGCKQIPFDITNNRNIRLNGTKQPVEFKSNQWFGATVRAHKEKVVACAPLYHWRSIKPISEKDPVGTCYIAIQNFSAYAEYSPCRSDLADPAGQGVCQAGFSLDFYKNGDLALGGPGSFYWQGQVFTASIADIIKDYSFKSIIRKISGEKQTKMAQSAYDDSYLGYSVAVGEFTDDSVQEVVAGVPRGAQNFGYVSIINSTDMTFIQNFTGDQMASYFGYTVAVSDVNNDGLDDILVGAPLFMDREFESNPREVGKVYLYMQESALEFRDPQVLKGTEVFGRFGSAIAHLGDLNQDGYNDIAVGSPFAGEDRKGRVFIYNGNSNGLNTDTSQILEGAWASQSMPAGFGFTLRGDSDIDKNDYPDLIVGAFGAGKAVVYRARPVITVDAQLLLHPMILNPENKTCQLPNSEFLVTCFTVRVCVTVSGQSIPSNIVVKADLELDWLKQKGAVKRTLFMESHQAHHSFDLIINKQRLSQCLDFLVYLREESEFRDKLTPISVNLNYSLDESAYLGDQELKPILNRYKKATIQEQAHILVDCGEDNMCIPDLKLSARPDKNQLVIGEENHILFIINTKNQGEGAYEAELRMIIPPEADYVGIERNMEGLRQLNCEYKMENESRLVVCDLGNPMVAGTNLSVGLRFVVQRLETVDAITFDLYIKSSNKDNPNSNFVSLQVNITAVAQVEIRGVSHPPQIILPIPNWEPKEKPSEEKDVGPLVQHIYELHNIGPSAISETQLNVGWPSQSRDEFLLYVFHIQTEGPLKCQAGSAINTLNIKPSEPEDTPELAAFLRNTSVSHAVSRRDVAVAEALRHIPTPTKLLNCTNVKCLMISCKVGHLGGGQRAVVKIRSRLWAPTFLQRKNDYYSLIANVSFEVKKMPYDIQPAKLPAATILIKTSVIWATPNISFAIPLWVILLAILLGILVLAILTLVMWKCGFFDRARPPQDDVADDTQQLTNKTAEA

Target ORF information:

RefSeq Version	XM_002939028.5
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis integrin subunit alpha 8 (itga8), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_002939028.5

ORF Insert Sequence:

ATGAAGTTGC TCAGTAACAC ACACAGCGCT GCTGCCGCCT CACTGCTCCG GGCTCTCCTT 
CCGCGCCACC TTTACCCACA GAAGATGCTG AGCCGGTGGC GCCCTATCGC CCAGCAGCCA 
GCATGCCCCA TGCTCATGGT GATAATGCTG AGTGGCGTTT GTTCTGCCTT TAACCTGGAT 
GTAGAGAAAG TGACAGTGTA CAGCGGCCCC CCGGATAGTT ACTTTGGCTA CTCTGTGGAC 
TTCTACTTGC CCGACTCGGC AAAAACTAGT ATTCTCATTG GAGCGCCCAA AGCCAATACC 
TCTCAGCCAC AGATAGTGGA AGGAGGAGCC GTGTATTACT GTCCCTTCCC CGATACCCTG 
CCTACTGGAT GCAAGCAGAT TCCATTTGAT ATCACAAACA ACAGGAACAT CAGGTTAAAT 
GGAACCAAAC AGCCTGTTGA ATTCAAGTCA AACCAATGGT TTGGTGCAAC AGTCAGGGCT 
CATAAGGAAA AAGTTGTGGC TTGTGCCCCA CTGTATCACT GGAGAAGTAT TAAGCCCATT 
TCAGAAAAGG ATCCTGTTGG CACTTGCTAC ATTGCCATTC AGAATTTCAG TGCTTATGCT 
GAATACTCTC CCTGCCGAAG TGACCTTGCT GATCCTGCTG GACAAGGCGT CTGTCAAGCT 
GGCTTCAGTC TGGATTTTTA TAAGAATGGA GACCTGGCGT TGGGAGGGCC CGGAAGTTTT 
TACTGGCAAG GACAAGTCTT TACTGCCAGT ATAGCAGACA TAATTAAAGA CTATTCCTTC 
AAGAGCATTA TCAGGAAGAT ATCGGGCGAG AAACAAACAA AAATGGCACA ATCTGCTTAC 
GATGATAGCT ACTTAGGATA CTCTGTGGCT GTTGGAGAAT TTACTGATGA TTCAGTGCAA 
GAGGTGGTTG CCGGAGTTCC AAGGGGAGCA CAAAACTTTG GATATGTCTC TATCATCAAT 
TCAACTGACA TGACCTTCAT TCAGAACTTT ACAGGTGACC AGATGGCCTC TTACTTTGGA 
TACACTGTTG CTGTGTCAGA TGTAAACAAT GACGGGTTGG ATGATATCTT AGTTGGAGCC 
CCACTTTTCA TGGATCGAGA ATTCGAGAGC AATCCAAGGG AGGTTGGAAA AGTCTACTTG 
TACATGCAAG AAAGCGCTCT TGAATTTAGA GACCCCCAAG TACTGAAAGG CACAGAAGTC 
TTTGGGAGAT TTGGTAGTGC TATTGCCCAT TTAGGCGATC TTAATCAAGA TGGATACAAT 
GACATTGCAG TTGGCTCACC ATTTGCGGGG GAAGACAGAA AAGGCAGAGT GTTTATCTAC 
AATGGGAACA GCAATGGGTT AAACACAGAT ACCTCCCAGA TACTGGAAGG AGCTTGGGCC 
TCGCAGTCAA TGCCTGCTGG ATTTGGCTTT ACGCTGAGAG GCGATTCAGA CATAGACAAA 
AACGATTACC CAGATCTTAT TGTGGGAGCT TTTGGAGCAG GAAAAGCTGT TGTTTATAGA 
GCGAGACCAG TGATCACTGT TGATGCTCAA CTTTTGCTCC ACCCCATGAT TCTGAACCCA 
GAGAATAAAA CCTGCCAGCT CCCCAATTCA GAATTTCTGG TGACTTGTTT CACTGTAAGA 
GTGTGTGTGA CTGTGTCTGG GCAAAGCATC CCTTCAAATA TTGTTGTGAA AGCTGACCTT 
GAACTGGATT GGTTAAAACA GAAGGGTGCA GTGAAAAGAA CACTTTTTAT GGAATCACAC 
CAGGCCCACC ATTCTTTTGA TCTGATCATC AACAAACAAA GATTATCCCA GTGCTTGGAC 
TTCCTTGTTT ATCTCAGAGA AGAAAGTGAA TTCCGCGATA AGCTCACTCC AATCAGTGTC 
AACTTAAATT ACAGTTTGGA TGAATCGGCT TATCTGGGTG ACCAGGAACT GAAACCGATC 
CTCAATCGTT ACAAGAAAGC AACCATTCAA GAACAGGCGC ACATTCTAGT GGACTGCGGA 
GAAGACAACA TGTGCATTCC TGATCTCAAG CTCTCCGCCA GACCGGACAA GAATCAGCTT 
GTTATTGGAG AAGAGAACCA CATTCTGTTC ATCATAAACA CAAAAAACCA AGGGGAAGGA 
GCTTATGAAG CTGAGCTGCG CATGATTATC CCACCAGAAG CTGATTATGT TGGAATTGAG 
CGCAACATGG AGGGACTTCG GCAACTTAAC TGTGAATATA AAATGGAAAA TGAATCTCGA 
CTGGTGGTAT GCGATCTTGG CAACCCTATG GTGGCTGGAA CAAATCTGTC TGTCGGTCTG 
AGATTCGTTG TGCAACGATT AGAAACCGTA GATGCCATCA CATTTGATCT CTACATTAAA 
AGTTCCAACA AGGACAATCC AAACAGTAAC TTTGTCAGCT TGCAAGTTAA CATAACCGCT 
GTGGCCCAAG TAGAAATTAG AGGAGTGTCC CATCCTCCTC AGATCATTCT ACCCATTCCT 
AACTGGGAGC CGAAGGAGAA ACCATCTGAA GAAAAGGACG TTGGGCCGTT AGTGCAGCAT 
ATATATGAGC TGCACAACAT TGGCCCGAGT GCTATAAGTG AAACACAGCT GAATGTGGGA 
TGGCCTTCTC AAAGCCGAGA TGAGTTTCTC CTCTATGTAT TTCACATTCA GACTGAGGGT 
CCCCTGAAGT GCCAGGCAGG AAGTGCCATT AACACCTTAA ATATCAAACC AAGTGAACCT 
GAAGATACCC CTGAGCTTGC AGCTTTTCTG AGGAATACCA GTGTATCTCA TGCAGTAAGC 
CGCCGGGATG TAGCTGTGGC AGAGGCGCTG AGGCACATTC CCACACCGAC AAAACTACTG 
AATTGCACTA ATGTGAAATG TTTAATGATA TCCTGCAAAG TCGGCCACTT GGGTGGAGGT 
CAGCGAGCAG TGGTAAAAAT CCGGTCTCGT CTGTGGGCTC CAACATTCTT ACAGAGAAAA 
AATGATTATT ACTCACTTAT TGCCAATGTG TCATTTGAAG TCAAGAAGAT GCCTTATGAC 
ATTCAGCCAG CCAAGCTCCC AGCAGCAACT ATACTGATAA AAACATCTGT GATCTGGGCC 
ACACCAAATA TCTCATTTGC CATCCCTCTT TGGGTCATCT TATTGGCTAT ACTCCTTGGT 
ATACTGGTTC TTGCCATTCT GACACTGGTT ATGTGGAAGT GCGGGTTCTT CGACAGAGCG 
AGGCCCCCAC AGGATGACGT TGCAGATGAC ACTCAACAGT TAACAAACAA GACGGCAGAA 
GCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

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itga8 cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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