bbx cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

Gene
Clones
gRNAs

Summary

Gene Symbol	bbx
Entrez Gene ID	100487237
Full Name	bobby sox homolog
General protein information	Preferred Names bobby sox homolog Names HMG box transcription factor BBX
Gene Type	protein-coding
Organism	Xenopus tropicalis(tropical clawed frog)

mRNA and Protein(s)

mRNA	Protein	Name
XM_012957573.2	XP_012813027.1	HMG box transcription factor BBX isoform X3
XM_012957573.3	XP_012813027.1	HMG box transcription factor BBX isoform X2
XM_002932287.3	XP_002932333.2	HMG box transcription factor BBX isoform X1
XM_002932287.4	XP_002932333.2	HMG box transcription factor BBX isoform X1
XM_012957574.2	XP_012813028.1	HMG box transcription factor BBX isoform X3
XM_018091607.1	XP_017947096.1	HMG box transcription factor BBX isoform X2

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	BBX	XP_001150832.1
Macaca mulatta (Rhesus monkey)	LOC704762	XP_002808361.1
Canis lupus familiaris (dog)	BBX	XP_005639519.1
Danio rerio (zebrafish)	LOC100005540	XP_005172755.1
Xenopus tropicalis (tropical clawed frog)	bbx	XP_002932333.2
Homo sapiens (human)	BBX	NP_001136040.1
Bos taurus (cattle)	BBX	NP_001179688.1
Mus musculus (house mouse)	Bbx	NP_081720.2
Rattus norvegicus (Norway rat)	Bbx	NP_001073407.1
Gallus gallus (chicken)	BBX	XP_004938341.1

Gene Ontology

The following bbx gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the bbx cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OXa05722	XM_002932287.4 Latest version!	Xenopus tropicalis bobby sox homolog (bbx), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
OXa33826	XM_012957573.3 Latest version!	Xenopus tropicalis bobby sox homolog (bbx), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
OXa33826	XM_012957574.2 Latest version!	Xenopus tropicalis bobby sox homolog (bbx), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
OXa42777	XM_018091607.1 Latest version!	Xenopus tropicalis bobby sox homolog (bbx), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
View Old Accession Versions >>
OXa33826	XM_012957573.2	Xenopus tropicalis bobby sox homolog (bbx), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
OXa05722	XM_002932287.3	Xenopus tropicalis bobby sox homolog (bbx), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

bbx ( XM_002932287.3 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_002932333.2 Xenopus tropicalis bobby sox homolog (bbx), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OXa05722
Clone ID Related Accession (Same CDS sequence)	XM_002932287.3 , XM_002932287.4
Accession Version	XM_002932287.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2889bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1473177600000
Organism	Xenopus tropicalis(tropical clawed frog)
Product	HMG box transcription factor BBX isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030678.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 23, 2015 this sequence version replaced XM_002932287.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACAGAGA GGAAGCCGAA AACACAGCGA ATTCGAATGA AGGAGCCATG GGGAATGAGC 
CGATCGGGGG TCACTCTCTG TAATGGAGAG CAGATCGGGT GTGCTGGCAG GAGGTCATTG 
TCACATGACA AAGAGATCAA AATAGAGTTA CCATTCTGTT CTTCCATTTG CCTTTCTTGG 
TGTATAGTCA TGAAAGGCAG TGTGAAAAAT AAGGACCATT CTACTGACGG AGAAGGAGCT 
GGAAAACGAC CAAAGAGAAA ATGCCTCCAA TGGCACCCAC TGCTTGCAAA CAAAATTCTT 
GACTTTTCTG AAGAAGATGA GGAAGAAGAG GATGAAGAAG ATATTGATAA GGTCCAGCTT 
CTTGATGGTG ATGGTCTAGA AAATGATGAA ACAGAAGACA ATGATTCTAG TGAACAAAGA 
GCACGGAGGC CAATGAACGC TTTCCTCTTA TTTTGCAAAC GTCATCGTTC CCTAGTTCGT 
CAGGAACATC CTCGTCTGGA TAATCGTGGT GCTACAAAAA TACTGGCAGA TTGGTGGGCC 
TATCTCGACC CTAATGAGAA GCAGAAGTAC ACAGATATGG CTAAAGAGTA CAAAGATGCA 
TTTATGAAAG CAAATCCTGG TTACAAGTGG TGTCCTACAA CTAATAAACC CGTCAAAGCT 
CATTCACCTA CTGTAACTAC CAGGAAAAAA CTGTGGTCCT TTAGCTCAGA AACTGTCAAA 
GATGTTCCAA GCCCCAAGAA AGTCCCACAA GAAGATGAAA TGCCACAGCT TAATTTTAGT 
ATTGCAGATC CCACACAAAT GGGAGGACTG AGTATGCTGC TTCTTGCTGG TGAACATGCC 
TTGACTGCAC ACGGGGATCA TGCTGTGACT GAAAGAGTGG AACATTTATT AAGTGATCAT 
GAGGTTTCTT CAGGTATTTG TCCTTTAACT GAATCAGGGT TTCCCCAGGA AAATAGAGAC 
ACTTCATTAT TTCAGTTTGA TGAGATATCT TCCAGCACAT CACATTCAGA TGACCCAGCA 
CCTGCTGAAC AAAGCGAGAA GTCAACACTG TTTCAGTTTG CCGAGATATC TTCAGATACT 
TCACAGACAC AGATTAATTG TGTGGATCCT GCAAAATGCT GTGGGAACTC TTCCCTTTTC 
CAATTAGCAG AGATATCTTC AAGTTCATCC CATTCTGATT CATCTGCATC AGCAAAGCAG 
TGTGGAACAT CAGCATTGTT TCAGCTTGCA GAGATGTGCG AGGCTTCTGA AGCTGTCAAA 
ATAGAAATAT CCAGGAATGT AGATACAGAC GATTTGGTAA GAACGCCTGT GTCCATAAAG 
GAAGAAATAG AAAGCTCATC AGAGGACCTG ATTTCTAGAA ACCTCAATGA TAGTTCACAT 
ATCTGTGAGA AAGCAGAAAC TAATAACATT AAAACTAAAA AGCACAAAGC ACACACCCTA 
GAACCTATAG ATGCTGAACT TTTAGGACTT CTGAAACGGG AAAAATTAAA AAAGAAGAAG 
AAGAAAAACA AATCAGACCA TGATGCTGCA GAGAAGGCAT CCCCAAAGAA AACCTGTAAA 
AAAAGGCAAT CATCGGAGTC TGATATTGAA AGTGTCATGT ACACTATAGA AGCTGTCGCA 
AAAGGTGATT GGGGCATTGA GCAAATTCCC ATTTCTCCAA GCAAGAAGGC ACGCATTTCT 
ACTAGCCCCA AACACAGCAT TTTTGACTTA AAGTCAGCAA AGAAAAAAGT TAAATCAAAA 
GAAAAAAAAA TGCAGAAAGA TAAGCCCTCA GACAGTACCA AAGAGCTGTC AGCGCCAGAA 
CACATAATTA CTGCACCAAT CATCGATTTA CCTTCCGAGA CTCCTGAAAG TGTCAAATCA 
GAGCCACCTT CTCCCACTGA GGAGCTGCCT CCCATGTGCT TGCCAGAAAT GCTTAAAACT 
GAGGACTTTA ACAGCAATAA AAAGACAGAA ACTTGTGGTT CAAGGAAATC AGAACGGTCT 
TGTAAAGGAG CTCTTTATAA GACACTGGTG TCTGAAGGCA TGCTGACATC ACTGCGGGCC 
AATGTTGACA GAGGTAAAAG AAACTCAGGA AAGGGCTGCT TATCAGACCA TGAAGGATGC 
TGGAATGAAG ACAGCTGGGC ATTCAATCAA ACTGGAACCA CTGGGAGTAA AAAGCTGAAA 
AAAACTAAAC CAAAGGAAGA GTCGACACTT GGATTAGCAA AGCTGGAAGA AGAGTTTGAA 
AAGAAGTTTA ACAGCCTTCC TCAGTACAGC CCTGTTACAT TTGATCGCAA AAATGCATCA 
CTTCCAAGGA AGAAGAAAAA GACTGGAAAT GGTATAGCTG AGCCACAAAA AGCCAATAAA 
GGTGTATTCC AGCCTCAGAA AAAGACCTTT TTTCACAAAA TTGTAAACAA GTACAAGCAC 
AAAAAAGACA AACAAATTAT TTCGGATAAA GCCATAGCAG AAGAAAAAAA CAACAGGGAG 
AACTCACCCA GAGCCAATAA TTTTGTATCA GCTTCCAGCA TTCCAGAATT TACAGCCATA 
CAGGAGCCTC TGGTAGGAAG CCAAAAAAGG AAAGCAAGAA AAACAAAGAT AACCCACCTT 
GTTAGATCTA TGGATGGTCA GACATCATTA AAAGGACAGA TTTCAGATGA AAAACCACTG 
TCTAGTATTT GTGAAGAAGA GGGGTCCTGC AGAGGTGAAC TCTTAATCTC TAGAGAAACT 
GAAGTGGAAC AGAATAATAG TATTGCCCAA GACATGCCTG CTGTGTCTGC ATTTTTCAGT 
CTTGCTGCTC TGGCCGAGGT AGCTGCAATG GAAAACGCTC ACAGAAGTCC AAGAGGATCT 
CTTTCCCGAG ATGGACAGAA GAAAGAAATG TCCCAGGCAG CTGTGCTGAT TTCATGTGCT 
GACCAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_002932333.2
CDS	1057..3945
Translation	MTERKPKTQRIRMKEPWGMSRSGVTLCNGEQIGCAGRRSLSHDKEIKIELPFCSSICLSWCIVMKGSVKNKDHSTDGEGAGKRPKRKCLQWHPLLANKILDFSEEDEEEEDEEDIDKVQLLDGDGLENDETEDNDSSEQRARRPMNAFLLFCKRHRSLVRQEHPRLDNRGATKILADWWAYLDPNEKQKYTDMAKEYKDAFMKANPGYKWCPTTNKPVKAHSPTVTTRKKLWSFSSETVKDVPSPKKVPQEDEMPQLNFSIADPTQMGGLSMLLLAGEHALTAHGDHAVTERVEHLLSDHEVSSGICPLTESGFPQENRDTSLFQFDEISSSTSHSDDPAPAEQSEKSTLFQFAEISSDTSQTQINCVDPAKCCGNSSLFQLAEISSSSSHSDSSASAKQCGTSALFQLAEMCEASEAVKIEISRNVDTDDLVRTPVSIKEEIESSSEDLISRNLNDSSHICEKAETNNIKTKKHKAHTLEPIDAELLGLLKREKLKKKKKKNKSDHDAAEKASPKKTCKKRQSSESDIESVMYTIEAVAKGDWGIEQIPISPSKKARISTSPKHSIFDLKSAKKKVKSKEKKMQKDKPSDSTKELSAPEHIITAPIIDLPSETPESVKSEPPSPTEELPPMCLPEMLKTEDFNSNKKTETCGSRKSERSCKGALYKTLVSEGMLTSLRANVDRGKRNSGKGCLSDHEGCWNEDSWAFNQTGTTGSKKLKKTKPKEESTLGLAKLEEEFEKKFNSLPQYSPVTFDRKNASLPRKKKKTGNGIAEPQKANKGVFQPQKKTFFHKIVNKYKHKKDKQIISDKAIAEEKNNRENSPRANNFVSASSIPEFTAIQEPLVGSQKRKARKTKITHLVRSMDGQTSLKGQISDEKPLSSICEEEGSCRGELLISRETEVEQNNSIAQDMPAVSAFFSLAALAEVAAMENAHRSPRGSLSRDGQKKEMSQAAVLISCADQ

Target ORF information:

RefSeq Version	XM_002932287.3
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis bobby sox homolog (bbx), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_002932287.3

ORF Insert Sequence:

ATGACAGAGA GGAAGCCGAA AACACAGCGA ATTCGAATGA AGGAGCCATG GGGAATGAGC 
CGATCGGGGG TCACTCTCTG TAATGGAGAG CAGATCGGGT GTGCTGGCAG GAGGTCATTG 
TCACATGACA AAGAGATCAA AATAGAGTTA CCATTCTGTT CTTCCATTTG CCTTTCTTGG 
TGTATAGTCA TGAAAGGCAG TGTGAAAAAT AAGGACCATT CTACTGACGG AGAAGGAGCT 
GGAAAACGAC CAAAGAGAAA ATGCCTCCAA TGGCACCCAC TGCTTGCAAA CAAAATTCTT 
GACTTTTCTG AAGAAGATGA GGAAGAAGAG GATGAAGAAG ATATTGATAA GGTCCAGCTT 
CTTGATGGTG ATGGTCTAGA AAATGATGAA ACAGAAGACA ATGATTCTAG TGAACAAAGA 
GCACGGAGGC CAATGAACGC TTTCCTCTTA TTTTGCAAAC GTCATCGTTC CCTAGTTCGT 
CAGGAACATC CTCGTCTGGA TAATCGTGGT GCTACAAAAA TACTGGCAGA TTGGTGGGCC 
TATCTCGACC CTAATGAGAA GCAGAAGTAC ACAGATATGG CTAAAGAGTA CAAAGATGCA 
TTTATGAAAG CAAATCCTGG TTACAAGTGG TGTCCTACAA CTAATAAACC CGTCAAAGCT 
CATTCACCTA CTGTAACTAC CAGGAAAAAA CTGTGGTCCT TTAGCTCAGA AACTGTCAAA 
GATGTTCCAA GCCCCAAGAA AGTCCCACAA GAAGATGAAA TGCCACAGCT TAATTTTAGT 
ATTGCAGATC CCACACAAAT GGGAGGACTG AGTATGCTGC TTCTTGCTGG TGAACATGCC 
TTGACTGCAC ACGGGGATCA TGCTGTGACT GAAAGAGTGG AACATTTATT AAGTGATCAT 
GAGGTTTCTT CAGGTATTTG TCCTTTAACT GAATCAGGGT TTCCCCAGGA AAATAGAGAC 
ACTTCATTAT TTCAGTTTGA TGAGATATCT TCCAGCACAT CACATTCAGA TGACCCAGCA 
CCTGCTGAAC AAAGCGAGAA GTCAACACTG TTTCAGTTTG CCGAGATATC TTCAGATACT 
TCACAGACAC AGATTAATTG TGTGGATCCT GCAAAATGCT GTGGGAACTC TTCCCTTTTC 
CAATTAGCAG AGATATCTTC AAGTTCATCC CATTCTGATT CATCTGCATC AGCAAAGCAG 
TGTGGAACAT CAGCATTGTT TCAGCTTGCA GAGATGTGCG AGGCTTCTGA AGCTGTCAAA 
ATAGAAATAT CCAGGAATGT AGATACAGAC GATTTGGTAA GAACGCCTGT GTCCATAAAG 
GAAGAAATAG AAAGCTCATC AGAGGACCTG ATTTCTAGAA ACCTCAATGA TAGTTCACAT 
ATCTGTGAGA AAGCAGAAAC TAATAACATT AAAACTAAAA AGCACAAAGC ACACACCCTA 
GAACCTATAG ATGCTGAACT TTTAGGACTT CTGAAACGGG AAAAATTAAA AAAGAAGAAG 
AAGAAAAACA AATCAGACCA TGATGCTGCA GAGAAGGCAT CCCCAAAGAA AACCTGTAAA 
AAAAGGCAAT CATCGGAGTC TGATATTGAA AGTGTCATGT ACACTATAGA AGCTGTCGCA 
AAAGGTGATT GGGGCATTGA GCAAATTCCC ATTTCTCCAA GCAAGAAGGC ACGCATTTCT 
ACTAGCCCCA AACACAGCAT TTTTGACTTA AAGTCAGCAA AGAAAAAAGT TAAATCAAAA 
GAAAAAAAAA TGCAGAAAGA TAAGCCCTCA GACAGTACCA AAGAGCTGTC AGCGCCAGAA 
CACATAATTA CTGCACCAAT CATCGATTTA CCTTCCGAGA CTCCTGAAAG TGTCAAATCA 
GAGCCACCTT CTCCCACTGA GGAGCTGCCT CCCATGTGCT TGCCAGAAAT GCTTAAAACT 
GAGGACTTTA ACAGCAATAA AAAGACAGAA ACTTGTGGTT CAAGGAAATC AGAACGGTCT 
TGTAAAGGAG CTCTTTATAA GACACTGGTG TCTGAAGGCA TGCTGACATC ACTGCGGGCC 
AATGTTGACA GAGGTAAAAG AAACTCAGGA AAGGGCTGCT TATCAGACCA TGAAGGATGC 
TGGAATGAAG ACAGCTGGGC ATTCAATCAA ACTGGAACCA CTGGGAGTAA AAAGCTGAAA 
AAAACTAAAC CAAAGGAAGA GTCGACACTT GGATTAGCAA AGCTGGAAGA AGAGTTTGAA 
AAGAAGTTTA ACAGCCTTCC TCAGTACAGC CCTGTTACAT TTGATCGCAA AAATGCATCA 
CTTCCAAGGA AGAAGAAAAA GACTGGAAAT GGTATAGCTG AGCCACAAAA AGCCAATAAA 
GGTGTATTCC AGCCTCAGAA AAAGACCTTT TTTCACAAAA TTGTAAACAA GTACAAGCAC 
AAAAAAGACA AACAAATTAT TTCGGATAAA GCCATAGCAG AAGAAAAAAA CAACAGGGAG 
AACTCACCCA GAGCCAATAA TTTTGTATCA GCTTCCAGCA TTCCAGAATT TACAGCCATA 
CAGGAGCCTC TGGTAGGAAG CCAAAAAAGG AAAGCAAGAA AAACAAAGAT AACCCACCTT 
GTTAGATCTA TGGATGGTCA GACATCATTA AAAGGACAGA TTTCAGATGA AAAACCACTG 
TCTAGTATTT GTGAAGAAGA GGGGTCCTGC AGAGGTGAAC TCTTAATCTC TAGAGAAACT 
GAAGTGGAAC AGAATAATAG TATTGCCCAA GACATGCCTG CTGTGTCTGC ATTTTTCAGT 
CTTGCTGCTC TGGCCGAGGT AGCTGCAATG GAAAACGCTC ACAGAAGTCC AAGAGGATCT 
CTTTCCCGAG ATGGACAGAA GAAAGAAATG TCCCAGGCAG CTGTGCTGAT TTCATGTGCT 
GACCAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

bbx ( XM_002932287.4 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_002932333.2 Xenopus tropicalis bobby sox homolog (bbx), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OXa05722
Clone ID Related Accession (Same CDS sequence)	XM_002932287.3 , XM_002932287.4
Accession Version	XM_002932287.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2889bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1576512000000
Organism	Xenopus tropicalis(tropical clawed frog)
Product	HMG box transcription factor BBX isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030678.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_002932287.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACAGAGA GGAAGCCGAA AACACAGCGA ATTCGAATGA AGGAGCCATG GGGAATGAGC 
CGATCGGGGG TCACTCTCTG TAATGGAGAG CAGATCGGGT GTGCTGGCAG GAGGTCATTG 
TCACATGACA AAGAGATCAA AATAGAGTTA CCATTCTGTT CTTCCATTTG CCTTTCTTGG 
TGTATAGTCA TGAAAGGCAG TGTGAAAAAT AAGGACCATT CTACTGACGG AGAAGGAGCT 
GGAAAACGAC CAAAGAGAAA ATGCCTCCAA TGGCACCCAC TGCTTGCAAA CAAAATTCTT 
GACTTTTCTG AAGAAGATGA GGAAGAAGAG GATGAAGAAG ATATTGATAA GGTCCAGCTT 
CTTGATGGTG ATGGTCTAGA AAATGATGAA ACAGAAGACA ATGATTCTAG TGAACAAAGA 
GCACGGAGGC CAATGAACGC TTTCCTCTTA TTTTGCAAAC GTCATCGTTC CCTAGTTCGT 
CAGGAACATC CTCGTCTGGA TAATCGTGGT GCTACAAAAA TACTGGCAGA TTGGTGGGCC 
TATCTCGACC CTAATGAGAA GCAGAAGTAC ACAGATATGG CTAAAGAGTA CAAAGATGCA 
TTTATGAAAG CAAATCCTGG TTACAAGTGG TGTCCTACAA CTAATAAACC CGTCAAAGCT 
CATTCACCTA CTGTAACTAC CAGGAAAAAA CTGTGGTCCT TTAGCTCAGA AACTGTCAAA 
GATGTTCCAA GCCCCAAGAA AGTCCCACAA GAAGATGAAA TGCCACAGCT TAATTTTAGT 
ATTGCAGATC CCACACAAAT GGGAGGACTG AGTATGCTGC TTCTTGCTGG TGAACATGCC 
TTGACTGCAC ACGGGGATCA TGCTGTGACT GAAAGAGTGG AACATTTATT AAGTGATCAT 
GAGGTTTCTT CAGGTATTTG TCCTTTAACT GAATCAGGGT TTCCCCAGGA AAATAGAGAC 
ACTTCATTAT TTCAGTTTGA TGAGATATCT TCCAGCACAT CACATTCAGA TGACCCAGCA 
CCTGCTGAAC AAAGCGAGAA GTCAACACTG TTTCAGTTTG CCGAGATATC TTCAGATACT 
TCACAGACAC AGATTAATTG TGTGGATCCT GCAAAATGCT GTGGGAACTC TTCCCTTTTC 
CAATTAGCAG AGATATCTTC AAGTTCATCC CATTCTGATT CATCTGCATC AGCAAAGCAG 
TGTGGAACAT CAGCATTGTT TCAGCTTGCA GAGATGTGCG AGGCTTCTGA AGCTGTCAAA 
ATAGAAATAT CCAGGAATGT AGATACAGAC GATTTGGTAA GAACGCCTGT GTCCATAAAG 
GAAGAAATAG AAAGCTCATC AGAGGACCTG ATTTCTAGAA ACCTCAATGA TAGTTCACAT 
ATCTGTGAGA AAGCAGAAAC TAATAACATT AAAACTAAAA AGCACAAAGC ACACACCCTA 
GAACCTATAG ATGCTGAACT TTTAGGACTT CTGAAACGGG AAAAATTAAA AAAGAAGAAG 
AAGAAAAACA AATCAGACCA TGATGCTGCA GAGAAGGCAT CCCCAAAGAA AACCTGTAAA 
AAAAGGCAAT CATCGGAGTC TGATATTGAA AGTGTCATGT ACACTATAGA AGCTGTCGCA 
AAAGGTGATT GGGGCATTGA GCAAATTCCC ATTTCTCCAA GCAAGAAGGC ACGCATTTCT 
ACTAGCCCCA AACACAGCAT TTTTGACTTA AAGTCAGCAA AGAAAAAAGT TAAATCAAAA 
GAAAAAAAAA TGCAGAAAGA TAAGCCCTCA GACAGTACCA AAGAGCTGTC AGCGCCAGAA 
CACATAATTA CTGCACCAAT CATCGATTTA CCTTCCGAGA CTCCTGAAAG TGTCAAATCA 
GAGCCACCTT CTCCCACTGA GGAGCTGCCT CCCATGTGCT TGCCAGAAAT GCTTAAAACT 
GAGGACTTTA ACAGCAATAA AAAGACAGAA ACTTGTGGTT CAAGGAAATC AGAACGGTCT 
TGTAAAGGAG CTCTTTATAA GACACTGGTG TCTGAAGGCA TGCTGACATC ACTGCGGGCC 
AATGTTGACA GAGGTAAAAG AAACTCAGGA AAGGGCTGCT TATCAGACCA TGAAGGATGC 
TGGAATGAAG ACAGCTGGGC ATTCAATCAA ACTGGAACCA CTGGGAGTAA AAAGCTGAAA 
AAAACTAAAC CAAAGGAAGA GTCGACACTT GGATTAGCAA AGCTGGAAGA AGAGTTTGAA 
AAGAAGTTTA ACAGCCTTCC TCAGTACAGC CCTGTTACAT TTGATCGCAA AAATGCATCA 
CTTCCAAGGA AGAAGAAAAA GACTGGAAAT GGTATAGCTG AGCCACAAAA AGCCAATAAA 
GGTGTATTCC AGCCTCAGAA AAAGACCTTT TTTCACAAAA TTGTAAACAA GTACAAGCAC 
AAAAAAGACA AACAAATTAT TTCGGATAAA GCCATAGCAG AAGAAAAAAA CAACAGGGAG 
AACTCACCCA GAGCCAATAA TTTTGTATCA GCTTCCAGCA TTCCAGAATT TACAGCCATA 
CAGGAGCCTC TGGTAGGAAG CCAAAAAAGG AAAGCAAGAA AAACAAAGAT AACCCACCTT 
GTTAGATCTA TGGATGGTCA GACATCATTA AAAGGACAGA TTTCAGATGA AAAACCACTG 
TCTAGTATTT GTGAAGAAGA GGGGTCCTGC AGAGGTGAAC TCTTAATCTC TAGAGAAACT 
GAAGTGGAAC AGAATAATAG TATTGCCCAA GACATGCCTG CTGTGTCTGC ATTTTTCAGT 
CTTGCTGCTC TGGCCGAGGT AGCTGCAATG GAAAACGCTC ACAGAAGTCC AAGAGGATCT 
CTTTCCCGAG ATGGACAGAA GAAAGAAATG TCCCAGGCAG CTGTGCTGAT TTCATGTGCT 
GACCAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_002932333.2
CDS	1004..3892
Translation	MTERKPKTQRIRMKEPWGMSRSGVTLCNGEQIGCAGRRSLSHDKEIKIELPFCSSICLSWCIVMKGSVKNKDHSTDGEGAGKRPKRKCLQWHPLLANKILDFSEEDEEEEDEEDIDKVQLLDGDGLENDETEDNDSSEQRARRPMNAFLLFCKRHRSLVRQEHPRLDNRGATKILADWWAYLDPNEKQKYTDMAKEYKDAFMKANPGYKWCPTTNKPVKAHSPTVTTRKKLWSFSSETVKDVPSPKKVPQEDEMPQLNFSIADPTQMGGLSMLLLAGEHALTAHGDHAVTERVEHLLSDHEVSSGICPLTESGFPQENRDTSLFQFDEISSSTSHSDDPAPAEQSEKSTLFQFAEISSDTSQTQINCVDPAKCCGNSSLFQLAEISSSSSHSDSSASAKQCGTSALFQLAEMCEASEAVKIEISRNVDTDDLVRTPVSIKEEIESSSEDLISRNLNDSSHICEKAETNNIKTKKHKAHTLEPIDAELLGLLKREKLKKKKKKNKSDHDAAEKASPKKTCKKRQSSESDIESVMYTIEAVAKGDWGIEQIPISPSKKARISTSPKHSIFDLKSAKKKVKSKEKKMQKDKPSDSTKELSAPEHIITAPIIDLPSETPESVKSEPPSPTEELPPMCLPEMLKTEDFNSNKKTETCGSRKSERSCKGALYKTLVSEGMLTSLRANVDRGKRNSGKGCLSDHEGCWNEDSWAFNQTGTTGSKKLKKTKPKEESTLGLAKLEEEFEKKFNSLPQYSPVTFDRKNASLPRKKKKTGNGIAEPQKANKGVFQPQKKTFFHKIVNKYKHKKDKQIISDKAIAEEKNNRENSPRANNFVSASSIPEFTAIQEPLVGSQKRKARKTKITHLVRSMDGQTSLKGQISDEKPLSSICEEEGSCRGELLISRETEVEQNNSIAQDMPAVSAFFSLAALAEVAAMENAHRSPRGSLSRDGQKKEMSQAAVLISCADQ

Target ORF information:

RefSeq Version	XM_002932287.4
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis bobby sox homolog (bbx), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_002932287.4

ORF Insert Sequence:

ATGACAGAGA GGAAGCCGAA AACACAGCGA ATTCGAATGA AGGAGCCATG GGGAATGAGC 
CGATCGGGGG TCACTCTCTG TAATGGAGAG CAGATCGGGT GTGCTGGCAG GAGGTCATTG 
TCACATGACA AAGAGATCAA AATAGAGTTA CCATTCTGTT CTTCCATTTG CCTTTCTTGG 
TGTATAGTCA TGAAAGGCAG TGTGAAAAAT AAGGACCATT CTACTGACGG AGAAGGAGCT 
GGAAAACGAC CAAAGAGAAA ATGCCTCCAA TGGCACCCAC TGCTTGCAAA CAAAATTCTT 
GACTTTTCTG AAGAAGATGA GGAAGAAGAG GATGAAGAAG ATATTGATAA GGTCCAGCTT 
CTTGATGGTG ATGGTCTAGA AAATGATGAA ACAGAAGACA ATGATTCTAG TGAACAAAGA 
GCACGGAGGC CAATGAACGC TTTCCTCTTA TTTTGCAAAC GTCATCGTTC CCTAGTTCGT 
CAGGAACATC CTCGTCTGGA TAATCGTGGT GCTACAAAAA TACTGGCAGA TTGGTGGGCC 
TATCTCGACC CTAATGAGAA GCAGAAGTAC ACAGATATGG CTAAAGAGTA CAAAGATGCA 
TTTATGAAAG CAAATCCTGG TTACAAGTGG TGTCCTACAA CTAATAAACC CGTCAAAGCT 
CATTCACCTA CTGTAACTAC CAGGAAAAAA CTGTGGTCCT TTAGCTCAGA AACTGTCAAA 
GATGTTCCAA GCCCCAAGAA AGTCCCACAA GAAGATGAAA TGCCACAGCT TAATTTTAGT 
ATTGCAGATC CCACACAAAT GGGAGGACTG AGTATGCTGC TTCTTGCTGG TGAACATGCC 
TTGACTGCAC ACGGGGATCA TGCTGTGACT GAAAGAGTGG AACATTTATT AAGTGATCAT 
GAGGTTTCTT CAGGTATTTG TCCTTTAACT GAATCAGGGT TTCCCCAGGA AAATAGAGAC 
ACTTCATTAT TTCAGTTTGA TGAGATATCT TCCAGCACAT CACATTCAGA TGACCCAGCA 
CCTGCTGAAC AAAGCGAGAA GTCAACACTG TTTCAGTTTG CCGAGATATC TTCAGATACT 
TCACAGACAC AGATTAATTG TGTGGATCCT GCAAAATGCT GTGGGAACTC TTCCCTTTTC 
CAATTAGCAG AGATATCTTC AAGTTCATCC CATTCTGATT CATCTGCATC AGCAAAGCAG 
TGTGGAACAT CAGCATTGTT TCAGCTTGCA GAGATGTGCG AGGCTTCTGA AGCTGTCAAA 
ATAGAAATAT CCAGGAATGT AGATACAGAC GATTTGGTAA GAACGCCTGT GTCCATAAAG 
GAAGAAATAG AAAGCTCATC AGAGGACCTG ATTTCTAGAA ACCTCAATGA TAGTTCACAT 
ATCTGTGAGA AAGCAGAAAC TAATAACATT AAAACTAAAA AGCACAAAGC ACACACCCTA 
GAACCTATAG ATGCTGAACT TTTAGGACTT CTGAAACGGG AAAAATTAAA AAAGAAGAAG 
AAGAAAAACA AATCAGACCA TGATGCTGCA GAGAAGGCAT CCCCAAAGAA AACCTGTAAA 
AAAAGGCAAT CATCGGAGTC TGATATTGAA AGTGTCATGT ACACTATAGA AGCTGTCGCA 
AAAGGTGATT GGGGCATTGA GCAAATTCCC ATTTCTCCAA GCAAGAAGGC ACGCATTTCT 
ACTAGCCCCA AACACAGCAT TTTTGACTTA AAGTCAGCAA AGAAAAAAGT TAAATCAAAA 
GAAAAAAAAA TGCAGAAAGA TAAGCCCTCA GACAGTACCA AAGAGCTGTC AGCGCCAGAA 
CACATAATTA CTGCACCAAT CATCGATTTA CCTTCCGAGA CTCCTGAAAG TGTCAAATCA 
GAGCCACCTT CTCCCACTGA GGAGCTGCCT CCCATGTGCT TGCCAGAAAT GCTTAAAACT 
GAGGACTTTA ACAGCAATAA AAAGACAGAA ACTTGTGGTT CAAGGAAATC AGAACGGTCT 
TGTAAAGGAG CTCTTTATAA GACACTGGTG TCTGAAGGCA TGCTGACATC ACTGCGGGCC 
AATGTTGACA GAGGTAAAAG AAACTCAGGA AAGGGCTGCT TATCAGACCA TGAAGGATGC 
TGGAATGAAG ACAGCTGGGC ATTCAATCAA ACTGGAACCA CTGGGAGTAA AAAGCTGAAA 
AAAACTAAAC CAAAGGAAGA GTCGACACTT GGATTAGCAA AGCTGGAAGA AGAGTTTGAA 
AAGAAGTTTA ACAGCCTTCC TCAGTACAGC CCTGTTACAT TTGATCGCAA AAATGCATCA 
CTTCCAAGGA AGAAGAAAAA GACTGGAAAT GGTATAGCTG AGCCACAAAA AGCCAATAAA 
GGTGTATTCC AGCCTCAGAA AAAGACCTTT TTTCACAAAA TTGTAAACAA GTACAAGCAC 
AAAAAAGACA AACAAATTAT TTCGGATAAA GCCATAGCAG AAGAAAAAAA CAACAGGGAG 
AACTCACCCA GAGCCAATAA TTTTGTATCA GCTTCCAGCA TTCCAGAATT TACAGCCATA 
CAGGAGCCTC TGGTAGGAAG CCAAAAAAGG AAAGCAAGAA AAACAAAGAT AACCCACCTT 
GTTAGATCTA TGGATGGTCA GACATCATTA AAAGGACAGA TTTCAGATGA AAAACCACTG 
TCTAGTATTT GTGAAGAAGA GGGGTCCTGC AGAGGTGAAC TCTTAATCTC TAGAGAAACT 
GAAGTGGAAC AGAATAATAG TATTGCCCAA GACATGCCTG CTGTGTCTGC ATTTTTCAGT 
CTTGCTGCTC TGGCCGAGGT AGCTGCAATG GAAAACGCTC ACAGAAGTCC AAGAGGATCT 
CTTTCCCGAG ATGGACAGAA GAAAGAAATG TCCCAGGCAG CTGTGCTGAT TTCATGTGCT 
GACCAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

bbx ( XM_012957573.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012813027.1 Xenopus tropicalis bobby sox homolog (bbx), transcript variant X3, mRNA.

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CloneID	OXa33826
Clone ID Related Accession (Same CDS sequence)	XM_012957573.2 , XM_012957573.3 , XM_012957574.2
Accession Version	XM_012957573.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2700bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1473177600000
Organism	Xenopus tropicalis(tropical clawed frog)
Product	HMG box transcription factor BBX isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030678.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012957573.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAAGGCA GTGTGAAAAA TAAGGACCAT TCTACTGACG GAGAAGGAGC TGGAAAACGA 
CCAAAGAGAA AATGCCTCCA ATGGCACCCA CTGCTTGCAA ACAAAATTCT TGACTTTTCT 
GAAGAAGATG AGGAAGAAGA GGATGAAGAA GATATTGATA AGGTCCAGCT TCTTGATGGT 
GATGGTCTAG AAAATGATGA AACAGAAGAC AATGATTCTA GTGAACAAAG AGCACGGAGG 
CCAATGAACG CTTTCCTCTT ATTTTGCAAA CGTCATCGTT CCCTAGTTCG TCAGGAACAT 
CCTCGTCTGG ATAATCGTGG TGCTACAAAA ATACTGGCAG ATTGGTGGGC CTATCTCGAC 
CCTAATGAGA AGCAGAAGTA CACAGATATG GCTAAAGAGT ACAAAGATGC ATTTATGAAA 
GCAAATCCTG GTTACAAGTG GTGTCCTACA ACTAATAAAC CCGTCAAAGC TCATTCACCT 
ACTGTAACTA CCAGGAAAAA ACTGTGGTCC TTTAGCTCAG AAACTGTCAA AGATGTTCCA 
AGCCCCAAGA AAGTCCCACA AGAAGATGAA ATGCCACAGC TTAATTTTAG TATTGCAGAT 
CCCACACAAA TGGGAGGACT GAGTATGCTG CTTCTTGCTG GTGAACATGC CTTGACTGCA 
CACGGGGATC ATGCTGTGAC TGAAAGAGTG GAACATTTAT TAAGTGATCA TGAGGTTTCT 
TCAGGTATTT GTCCTTTAAC TGAATCAGGG TTTCCCCAGG AAAATAGAGA CACTTCATTA 
TTTCAGTTTG ATGAGATATC TTCCAGCACA TCACATTCAG ATGACCCAGC ACCTGCTGAA 
CAAAGCGAGA AGTCAACACT GTTTCAGTTT GCCGAGATAT CTTCAGATAC TTCACAGACA 
CAGATTAATT GTGTGGATCC TGCAAAATGC TGTGGGAACT CTTCCCTTTT CCAATTAGCA 
GAGATATCTT CAAGTTCATC CCATTCTGAT TCATCTGCAT CAGCAAAGCA GTGTGGAACA 
TCAGCATTGT TTCAGCTTGC AGAGATGTGC GAGGCTTCTG AAGCTGTCAA AATAGAAATA 
TCCAGGAATG TAGATACAGA CGATTTGGTA AGAACGCCTG TGTCCATAAA GGAAGAAATA 
GAAAGCTCAT CAGAGGACCT GATTTCTAGA AACCTCAATG ATAGTTCACA TATCTGTGAG 
AAAGCAGAAA CTAATAACAT TAAAACTAAA AAGCACAAAG CACACACCCT AGAACCTATA 
GATGCTGAAC TTTTAGGACT TCTGAAACGG GAAAAATTAA AAAAGAAGAA GAAGAAAAAC 
AAATCAGACC ATGATGCTGC AGAGAAGGCA TCCCCAAAGA AAACCTGTAA AAAAAGGCAA 
TCATCGGAGT CTGATATTGA AAGTGTCATG TACACTATAG AAGCTGTCGC AAAAGGTGAT 
TGGGGCATTG AGCAAATTCC CATTTCTCCA AGCAAGAAGG CACGCATTTC TACTAGCCCC 
AAACACAGCA TTTTTGACTT AAAGTCAGCA AAGAAAAAAG TTAAATCAAA AGAAAAAAAA 
ATGCAGAAAG ATAAGCCCTC AGACAGTACC AAAGAGCTGT CAGCGCCAGA ACACATAATT 
ACTGCACCAA TCATCGATTT ACCTTCCGAG ACTCCTGAAA GTGTCAAATC AGAGCCACCT 
TCTCCCACTG AGGAGCTGCC TCCCATGTGC TTGCCAGAAA TGCTTAAAAC TGAGGACTTT 
AACAGCAATA AAAAGACAGA AACTTGTGGT TCAAGGAAAT CAGAACGGTC TTGTAAAGGA 
GCTCTTTATA AGACACTGGT GTCTGAAGGC ATGCTGACAT CACTGCGGGC CAATGTTGAC 
AGAGGTAAAA GAAACTCAGG AAAGGGCTGC TTATCAGACC ATGAAGGATG CTGGAATGAA 
GACAGCTGGG CATTCAATCA AACTGGAACC ACTGGGAGTA AAAAGCTGAA AAAAACTAAA 
CCAAAGGAAG AGTCGACACT TGGATTAGCA AAGCTGGAAG AAGAGTTTGA AAAGAAGTTT 
AACAGCCTTC CTCAGTACAG CCCTGTTACA TTTGATCGCA AAAATGCATC ACTTCCAAGG 
AAGAAGAAAA AGACTGGAAA TGGTATAGCT GAGCCACAAA AAGCCAATAA AGGTGTATTC 
CAGCCTCAGA AAAAGACCTT TTTTCACAAA ATTGTAAACA AGTACAAGCA CAAAAAAGAC 
AAACAAATTA TTTCGGATAA AGCCATAGCA GAAGAAAAAA ACAACAGGGA GAACTCACCC 
AGAGCCAATA ATTTTGTATC AGCTTCCAGC ATTCCAGAAT TTACAGCCAT ACAGGAGCCT 
CTGGTAGGAA GCCAAAAAAG GAAAGCAAGA AAAACAAAGA TAACCCACCT TGTTAGATCT 
ATGGATGGTC AGACATCATT AAAAGGACAG ATTTCAGATG AAAAACCACT GTCTAGTATT 
TGTGAAGAAG AGGGGTCCTG CAGAGGTGAA CTCTTAATCT CTAGAGAAAC TGAAGTGGAA 
CAGAATAATA GTATTGCCCA AGACATGCCT GCTGTGTCTG CATTTTTCAG TCTTGCTGCT 
CTGGCCGAGG TAGCTGCAAT GGAAAACGCT CACAGAAGTC CAAGAGGATC TCTTTCCCGA 
GATGGACAGA AGAAAGAAAT GTCCCAGGCA GCTGTGCTGA TTTCATGTGC TGACCAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012813027.1
CDS	1035..3734
Translation	MKGSVKNKDHSTDGEGAGKRPKRKCLQWHPLLANKILDFSEEDEEEEDEEDIDKVQLLDGDGLENDETEDNDSSEQRARRPMNAFLLFCKRHRSLVRQEHPRLDNRGATKILADWWAYLDPNEKQKYTDMAKEYKDAFMKANPGYKWCPTTNKPVKAHSPTVTTRKKLWSFSSETVKDVPSPKKVPQEDEMPQLNFSIADPTQMGGLSMLLLAGEHALTAHGDHAVTERVEHLLSDHEVSSGICPLTESGFPQENRDTSLFQFDEISSSTSHSDDPAPAEQSEKSTLFQFAEISSDTSQTQINCVDPAKCCGNSSLFQLAEISSSSSHSDSSASAKQCGTSALFQLAEMCEASEAVKIEISRNVDTDDLVRTPVSIKEEIESSSEDLISRNLNDSSHICEKAETNNIKTKKHKAHTLEPIDAELLGLLKREKLKKKKKKNKSDHDAAEKASPKKTCKKRQSSESDIESVMYTIEAVAKGDWGIEQIPISPSKKARISTSPKHSIFDLKSAKKKVKSKEKKMQKDKPSDSTKELSAPEHIITAPIIDLPSETPESVKSEPPSPTEELPPMCLPEMLKTEDFNSNKKTETCGSRKSERSCKGALYKTLVSEGMLTSLRANVDRGKRNSGKGCLSDHEGCWNEDSWAFNQTGTTGSKKLKKTKPKEESTLGLAKLEEEFEKKFNSLPQYSPVTFDRKNASLPRKKKKTGNGIAEPQKANKGVFQPQKKTFFHKIVNKYKHKKDKQIISDKAIAEEKNNRENSPRANNFVSASSIPEFTAIQEPLVGSQKRKARKTKITHLVRSMDGQTSLKGQISDEKPLSSICEEEGSCRGELLISRETEVEQNNSIAQDMPAVSAFFSLAALAEVAAMENAHRSPRGSLSRDGQKKEMSQAAVLISCADQ

Target ORF information:

RefSeq Version	XM_012957573.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis bobby sox homolog (bbx), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012957573.2

ORF Insert Sequence:

ATGAAAGGCA GTGTGAAAAA TAAGGACCAT TCTACTGACG GAGAAGGAGC TGGAAAACGA 
CCAAAGAGAA AATGCCTCCA ATGGCACCCA CTGCTTGCAA ACAAAATTCT TGACTTTTCT 
GAAGAAGATG AGGAAGAAGA GGATGAAGAA GATATTGATA AGGTCCAGCT TCTTGATGGT 
GATGGTCTAG AAAATGATGA AACAGAAGAC AATGATTCTA GTGAACAAAG AGCACGGAGG 
CCAATGAACG CTTTCCTCTT ATTTTGCAAA CGTCATCGTT CCCTAGTTCG TCAGGAACAT 
CCTCGTCTGG ATAATCGTGG TGCTACAAAA ATACTGGCAG ATTGGTGGGC CTATCTCGAC 
CCTAATGAGA AGCAGAAGTA CACAGATATG GCTAAAGAGT ACAAAGATGC ATTTATGAAA 
GCAAATCCTG GTTACAAGTG GTGTCCTACA ACTAATAAAC CCGTCAAAGC TCATTCACCT 
ACTGTAACTA CCAGGAAAAA ACTGTGGTCC TTTAGCTCAG AAACTGTCAA AGATGTTCCA 
AGCCCCAAGA AAGTCCCACA AGAAGATGAA ATGCCACAGC TTAATTTTAG TATTGCAGAT 
CCCACACAAA TGGGAGGACT GAGTATGCTG CTTCTTGCTG GTGAACATGC CTTGACTGCA 
CACGGGGATC ATGCTGTGAC TGAAAGAGTG GAACATTTAT TAAGTGATCA TGAGGTTTCT 
TCAGGTATTT GTCCTTTAAC TGAATCAGGG TTTCCCCAGG AAAATAGAGA CACTTCATTA 
TTTCAGTTTG ATGAGATATC TTCCAGCACA TCACATTCAG ATGACCCAGC ACCTGCTGAA 
CAAAGCGAGA AGTCAACACT GTTTCAGTTT GCCGAGATAT CTTCAGATAC TTCACAGACA 
CAGATTAATT GTGTGGATCC TGCAAAATGC TGTGGGAACT CTTCCCTTTT CCAATTAGCA 
GAGATATCTT CAAGTTCATC CCATTCTGAT TCATCTGCAT CAGCAAAGCA GTGTGGAACA 
TCAGCATTGT TTCAGCTTGC AGAGATGTGC GAGGCTTCTG AAGCTGTCAA AATAGAAATA 
TCCAGGAATG TAGATACAGA CGATTTGGTA AGAACGCCTG TGTCCATAAA GGAAGAAATA 
GAAAGCTCAT CAGAGGACCT GATTTCTAGA AACCTCAATG ATAGTTCACA TATCTGTGAG 
AAAGCAGAAA CTAATAACAT TAAAACTAAA AAGCACAAAG CACACACCCT AGAACCTATA 
GATGCTGAAC TTTTAGGACT TCTGAAACGG GAAAAATTAA AAAAGAAGAA GAAGAAAAAC 
AAATCAGACC ATGATGCTGC AGAGAAGGCA TCCCCAAAGA AAACCTGTAA AAAAAGGCAA 
TCATCGGAGT CTGATATTGA AAGTGTCATG TACACTATAG AAGCTGTCGC AAAAGGTGAT 
TGGGGCATTG AGCAAATTCC CATTTCTCCA AGCAAGAAGG CACGCATTTC TACTAGCCCC 
AAACACAGCA TTTTTGACTT AAAGTCAGCA AAGAAAAAAG TTAAATCAAA AGAAAAAAAA 
ATGCAGAAAG ATAAGCCCTC AGACAGTACC AAAGAGCTGT CAGCGCCAGA ACACATAATT 
ACTGCACCAA TCATCGATTT ACCTTCCGAG ACTCCTGAAA GTGTCAAATC AGAGCCACCT 
TCTCCCACTG AGGAGCTGCC TCCCATGTGC TTGCCAGAAA TGCTTAAAAC TGAGGACTTT 
AACAGCAATA AAAAGACAGA AACTTGTGGT TCAAGGAAAT CAGAACGGTC TTGTAAAGGA 
GCTCTTTATA AGACACTGGT GTCTGAAGGC ATGCTGACAT CACTGCGGGC CAATGTTGAC 
AGAGGTAAAA GAAACTCAGG AAAGGGCTGC TTATCAGACC ATGAAGGATG CTGGAATGAA 
GACAGCTGGG CATTCAATCA AACTGGAACC ACTGGGAGTA AAAAGCTGAA AAAAACTAAA 
CCAAAGGAAG AGTCGACACT TGGATTAGCA AAGCTGGAAG AAGAGTTTGA AAAGAAGTTT 
AACAGCCTTC CTCAGTACAG CCCTGTTACA TTTGATCGCA AAAATGCATC ACTTCCAAGG 
AAGAAGAAAA AGACTGGAAA TGGTATAGCT GAGCCACAAA AAGCCAATAA AGGTGTATTC 
CAGCCTCAGA AAAAGACCTT TTTTCACAAA ATTGTAAACA AGTACAAGCA CAAAAAAGAC 
AAACAAATTA TTTCGGATAA AGCCATAGCA GAAGAAAAAA ACAACAGGGA GAACTCACCC 
AGAGCCAATA ATTTTGTATC AGCTTCCAGC ATTCCAGAAT TTACAGCCAT ACAGGAGCCT 
CTGGTAGGAA GCCAAAAAAG GAAAGCAAGA AAAACAAAGA TAACCCACCT TGTTAGATCT 
ATGGATGGTC AGACATCATT AAAAGGACAG ATTTCAGATG AAAAACCACT GTCTAGTATT 
TGTGAAGAAG AGGGGTCCTG CAGAGGTGAA CTCTTAATCT CTAGAGAAAC TGAAGTGGAA 
CAGAATAATA GTATTGCCCA AGACATGCCT GCTGTGTCTG CATTTTTCAG TCTTGCTGCT 
CTGGCCGAGG TAGCTGCAAT GGAAAACGCT CACAGAAGTC CAAGAGGATC TCTTTCCCGA 
GATGGACAGA AGAAAGAAAT GTCCCAGGCA GCTGTGCTGA TTTCATGTGC TGACCAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

bbx ( XM_012957573.3 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012813027.1 Xenopus tropicalis bobby sox homolog (bbx), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OXa33826
Clone ID Related Accession (Same CDS sequence)	XM_012957573.2 , XM_012957573.3 , XM_012957574.2
Accession Version	XM_012957573.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2700bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1576512000000
Organism	Xenopus tropicalis(tropical clawed frog)
Product	HMG box transcription factor BBX isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030678.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012957573.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAAGGCA GTGTGAAAAA TAAGGACCAT TCTACTGACG GAGAAGGAGC TGGAAAACGA 
CCAAAGAGAA AATGCCTCCA ATGGCACCCA CTGCTTGCAA ACAAAATTCT TGACTTTTCT 
GAAGAAGATG AGGAAGAAGA GGATGAAGAA GATATTGATA AGGTCCAGCT TCTTGATGGT 
GATGGTCTAG AAAATGATGA AACAGAAGAC AATGATTCTA GTGAACAAAG AGCACGGAGG 
CCAATGAACG CTTTCCTCTT ATTTTGCAAA CGTCATCGTT CCCTAGTTCG TCAGGAACAT 
CCTCGTCTGG ATAATCGTGG TGCTACAAAA ATACTGGCAG ATTGGTGGGC CTATCTCGAC 
CCTAATGAGA AGCAGAAGTA CACAGATATG GCTAAAGAGT ACAAAGATGC ATTTATGAAA 
GCAAATCCTG GTTACAAGTG GTGTCCTACA ACTAATAAAC CCGTCAAAGC TCATTCACCT 
ACTGTAACTA CCAGGAAAAA ACTGTGGTCC TTTAGCTCAG AAACTGTCAA AGATGTTCCA 
AGCCCCAAGA AAGTCCCACA AGAAGATGAA ATGCCACAGC TTAATTTTAG TATTGCAGAT 
CCCACACAAA TGGGAGGACT GAGTATGCTG CTTCTTGCTG GTGAACATGC CTTGACTGCA 
CACGGGGATC ATGCTGTGAC TGAAAGAGTG GAACATTTAT TAAGTGATCA TGAGGTTTCT 
TCAGGTATTT GTCCTTTAAC TGAATCAGGG TTTCCCCAGG AAAATAGAGA CACTTCATTA 
TTTCAGTTTG ATGAGATATC TTCCAGCACA TCACATTCAG ATGACCCAGC ACCTGCTGAA 
CAAAGCGAGA AGTCAACACT GTTTCAGTTT GCCGAGATAT CTTCAGATAC TTCACAGACA 
CAGATTAATT GTGTGGATCC TGCAAAATGC TGTGGGAACT CTTCCCTTTT CCAATTAGCA 
GAGATATCTT CAAGTTCATC CCATTCTGAT TCATCTGCAT CAGCAAAGCA GTGTGGAACA 
TCAGCATTGT TTCAGCTTGC AGAGATGTGC GAGGCTTCTG AAGCTGTCAA AATAGAAATA 
TCCAGGAATG TAGATACAGA CGATTTGGTA AGAACGCCTG TGTCCATAAA GGAAGAAATA 
GAAAGCTCAT CAGAGGACCT GATTTCTAGA AACCTCAATG ATAGTTCACA TATCTGTGAG 
AAAGCAGAAA CTAATAACAT TAAAACTAAA AAGCACAAAG CACACACCCT AGAACCTATA 
GATGCTGAAC TTTTAGGACT TCTGAAACGG GAAAAATTAA AAAAGAAGAA GAAGAAAAAC 
AAATCAGACC ATGATGCTGC AGAGAAGGCA TCCCCAAAGA AAACCTGTAA AAAAAGGCAA 
TCATCGGAGT CTGATATTGA AAGTGTCATG TACACTATAG AAGCTGTCGC AAAAGGTGAT 
TGGGGCATTG AGCAAATTCC CATTTCTCCA AGCAAGAAGG CACGCATTTC TACTAGCCCC 
AAACACAGCA TTTTTGACTT AAAGTCAGCA AAGAAAAAAG TTAAATCAAA AGAAAAAAAA 
ATGCAGAAAG ATAAGCCCTC AGACAGTACC AAAGAGCTGT CAGCGCCAGA ACACATAATT 
ACTGCACCAA TCATCGATTT ACCTTCCGAG ACTCCTGAAA GTGTCAAATC AGAGCCACCT 
TCTCCCACTG AGGAGCTGCC TCCCATGTGC TTGCCAGAAA TGCTTAAAAC TGAGGACTTT 
AACAGCAATA AAAAGACAGA AACTTGTGGT TCAAGGAAAT CAGAACGGTC TTGTAAAGGA 
GCTCTTTATA AGACACTGGT GTCTGAAGGC ATGCTGACAT CACTGCGGGC CAATGTTGAC 
AGAGGTAAAA GAAACTCAGG AAAGGGCTGC TTATCAGACC ATGAAGGATG CTGGAATGAA 
GACAGCTGGG CATTCAATCA AACTGGAACC ACTGGGAGTA AAAAGCTGAA AAAAACTAAA 
CCAAAGGAAG AGTCGACACT TGGATTAGCA AAGCTGGAAG AAGAGTTTGA AAAGAAGTTT 
AACAGCCTTC CTCAGTACAG CCCTGTTACA TTTGATCGCA AAAATGCATC ACTTCCAAGG 
AAGAAGAAAA AGACTGGAAA TGGTATAGCT GAGCCACAAA AAGCCAATAA AGGTGTATTC 
CAGCCTCAGA AAAAGACCTT TTTTCACAAA ATTGTAAACA AGTACAAGCA CAAAAAAGAC 
AAACAAATTA TTTCGGATAA AGCCATAGCA GAAGAAAAAA ACAACAGGGA GAACTCACCC 
AGAGCCAATA ATTTTGTATC AGCTTCCAGC ATTCCAGAAT TTACAGCCAT ACAGGAGCCT 
CTGGTAGGAA GCCAAAAAAG GAAAGCAAGA AAAACAAAGA TAACCCACCT TGTTAGATCT 
ATGGATGGTC AGACATCATT AAAAGGACAG ATTTCAGATG AAAAACCACT GTCTAGTATT 
TGTGAAGAAG AGGGGTCCTG CAGAGGTGAA CTCTTAATCT CTAGAGAAAC TGAAGTGGAA 
CAGAATAATA GTATTGCCCA AGACATGCCT GCTGTGTCTG CATTTTTCAG TCTTGCTGCT 
CTGGCCGAGG TAGCTGCAAT GGAAAACGCT CACAGAAGTC CAAGAGGATC TCTTTCCCGA 
GATGGACAGA AGAAAGAAAT GTCCCAGGCA GCTGTGCTGA TTTCATGTGC TGACCAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012813027.1
CDS	287..2986
Translation	MKGSVKNKDHSTDGEGAGKRPKRKCLQWHPLLANKILDFSEEDEEEEDEEDIDKVQLLDGDGLENDETEDNDSSEQRARRPMNAFLLFCKRHRSLVRQEHPRLDNRGATKILADWWAYLDPNEKQKYTDMAKEYKDAFMKANPGYKWCPTTNKPVKAHSPTVTTRKKLWSFSSETVKDVPSPKKVPQEDEMPQLNFSIADPTQMGGLSMLLLAGEHALTAHGDHAVTERVEHLLSDHEVSSGICPLTESGFPQENRDTSLFQFDEISSSTSHSDDPAPAEQSEKSTLFQFAEISSDTSQTQINCVDPAKCCGNSSLFQLAEISSSSSHSDSSASAKQCGTSALFQLAEMCEASEAVKIEISRNVDTDDLVRTPVSIKEEIESSSEDLISRNLNDSSHICEKAETNNIKTKKHKAHTLEPIDAELLGLLKREKLKKKKKKNKSDHDAAEKASPKKTCKKRQSSESDIESVMYTIEAVAKGDWGIEQIPISPSKKARISTSPKHSIFDLKSAKKKVKSKEKKMQKDKPSDSTKELSAPEHIITAPIIDLPSETPESVKSEPPSPTEELPPMCLPEMLKTEDFNSNKKTETCGSRKSERSCKGALYKTLVSEGMLTSLRANVDRGKRNSGKGCLSDHEGCWNEDSWAFNQTGTTGSKKLKKTKPKEESTLGLAKLEEEFEKKFNSLPQYSPVTFDRKNASLPRKKKKTGNGIAEPQKANKGVFQPQKKTFFHKIVNKYKHKKDKQIISDKAIAEEKNNRENSPRANNFVSASSIPEFTAIQEPLVGSQKRKARKTKITHLVRSMDGQTSLKGQISDEKPLSSICEEEGSCRGELLISRETEVEQNNSIAQDMPAVSAFFSLAALAEVAAMENAHRSPRGSLSRDGQKKEMSQAAVLISCADQ

Target ORF information:

RefSeq Version	XM_012957573.3
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis bobby sox homolog (bbx), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012957573.3

ORF Insert Sequence:

ATGAAAGGCA GTGTGAAAAA TAAGGACCAT TCTACTGACG GAGAAGGAGC TGGAAAACGA 
CCAAAGAGAA AATGCCTCCA ATGGCACCCA CTGCTTGCAA ACAAAATTCT TGACTTTTCT 
GAAGAAGATG AGGAAGAAGA GGATGAAGAA GATATTGATA AGGTCCAGCT TCTTGATGGT 
GATGGTCTAG AAAATGATGA AACAGAAGAC AATGATTCTA GTGAACAAAG AGCACGGAGG 
CCAATGAACG CTTTCCTCTT ATTTTGCAAA CGTCATCGTT CCCTAGTTCG TCAGGAACAT 
CCTCGTCTGG ATAATCGTGG TGCTACAAAA ATACTGGCAG ATTGGTGGGC CTATCTCGAC 
CCTAATGAGA AGCAGAAGTA CACAGATATG GCTAAAGAGT ACAAAGATGC ATTTATGAAA 
GCAAATCCTG GTTACAAGTG GTGTCCTACA ACTAATAAAC CCGTCAAAGC TCATTCACCT 
ACTGTAACTA CCAGGAAAAA ACTGTGGTCC TTTAGCTCAG AAACTGTCAA AGATGTTCCA 
AGCCCCAAGA AAGTCCCACA AGAAGATGAA ATGCCACAGC TTAATTTTAG TATTGCAGAT 
CCCACACAAA TGGGAGGACT GAGTATGCTG CTTCTTGCTG GTGAACATGC CTTGACTGCA 
CACGGGGATC ATGCTGTGAC TGAAAGAGTG GAACATTTAT TAAGTGATCA TGAGGTTTCT 
TCAGGTATTT GTCCTTTAAC TGAATCAGGG TTTCCCCAGG AAAATAGAGA CACTTCATTA 
TTTCAGTTTG ATGAGATATC TTCCAGCACA TCACATTCAG ATGACCCAGC ACCTGCTGAA 
CAAAGCGAGA AGTCAACACT GTTTCAGTTT GCCGAGATAT CTTCAGATAC TTCACAGACA 
CAGATTAATT GTGTGGATCC TGCAAAATGC TGTGGGAACT CTTCCCTTTT CCAATTAGCA 
GAGATATCTT CAAGTTCATC CCATTCTGAT TCATCTGCAT CAGCAAAGCA GTGTGGAACA 
TCAGCATTGT TTCAGCTTGC AGAGATGTGC GAGGCTTCTG AAGCTGTCAA AATAGAAATA 
TCCAGGAATG TAGATACAGA CGATTTGGTA AGAACGCCTG TGTCCATAAA GGAAGAAATA 
GAAAGCTCAT CAGAGGACCT GATTTCTAGA AACCTCAATG ATAGTTCACA TATCTGTGAG 
AAAGCAGAAA CTAATAACAT TAAAACTAAA AAGCACAAAG CACACACCCT AGAACCTATA 
GATGCTGAAC TTTTAGGACT TCTGAAACGG GAAAAATTAA AAAAGAAGAA GAAGAAAAAC 
AAATCAGACC ATGATGCTGC AGAGAAGGCA TCCCCAAAGA AAACCTGTAA AAAAAGGCAA 
TCATCGGAGT CTGATATTGA AAGTGTCATG TACACTATAG AAGCTGTCGC AAAAGGTGAT 
TGGGGCATTG AGCAAATTCC CATTTCTCCA AGCAAGAAGG CACGCATTTC TACTAGCCCC 
AAACACAGCA TTTTTGACTT AAAGTCAGCA AAGAAAAAAG TTAAATCAAA AGAAAAAAAA 
ATGCAGAAAG ATAAGCCCTC AGACAGTACC AAAGAGCTGT CAGCGCCAGA ACACATAATT 
ACTGCACCAA TCATCGATTT ACCTTCCGAG ACTCCTGAAA GTGTCAAATC AGAGCCACCT 
TCTCCCACTG AGGAGCTGCC TCCCATGTGC TTGCCAGAAA TGCTTAAAAC TGAGGACTTT 
AACAGCAATA AAAAGACAGA AACTTGTGGT TCAAGGAAAT CAGAACGGTC TTGTAAAGGA 
GCTCTTTATA AGACACTGGT GTCTGAAGGC ATGCTGACAT CACTGCGGGC CAATGTTGAC 
AGAGGTAAAA GAAACTCAGG AAAGGGCTGC TTATCAGACC ATGAAGGATG CTGGAATGAA 
GACAGCTGGG CATTCAATCA AACTGGAACC ACTGGGAGTA AAAAGCTGAA AAAAACTAAA 
CCAAAGGAAG AGTCGACACT TGGATTAGCA AAGCTGGAAG AAGAGTTTGA AAAGAAGTTT 
AACAGCCTTC CTCAGTACAG CCCTGTTACA TTTGATCGCA AAAATGCATC ACTTCCAAGG 
AAGAAGAAAA AGACTGGAAA TGGTATAGCT GAGCCACAAA AAGCCAATAA AGGTGTATTC 
CAGCCTCAGA AAAAGACCTT TTTTCACAAA ATTGTAAACA AGTACAAGCA CAAAAAAGAC 
AAACAAATTA TTTCGGATAA AGCCATAGCA GAAGAAAAAA ACAACAGGGA GAACTCACCC 
AGAGCCAATA ATTTTGTATC AGCTTCCAGC ATTCCAGAAT TTACAGCCAT ACAGGAGCCT 
CTGGTAGGAA GCCAAAAAAG GAAAGCAAGA AAAACAAAGA TAACCCACCT TGTTAGATCT 
ATGGATGGTC AGACATCATT AAAAGGACAG ATTTCAGATG AAAAACCACT GTCTAGTATT 
TGTGAAGAAG AGGGGTCCTG CAGAGGTGAA CTCTTAATCT CTAGAGAAAC TGAAGTGGAA 
CAGAATAATA GTATTGCCCA AGACATGCCT GCTGTGTCTG CATTTTTCAG TCTTGCTGCT 
CTGGCCGAGG TAGCTGCAAT GGAAAACGCT CACAGAAGTC CAAGAGGATC TCTTTCCCGA 
GATGGACAGA AGAAAGAAAT GTCCCAGGCA GCTGTGCTGA TTTCATGTGC TGACCAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

bbx ( XM_012957574.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012813028.1 Xenopus tropicalis bobby sox homolog (bbx), transcript variant X4, mRNA.

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CloneID	OXa33826
Clone ID Related Accession (Same CDS sequence)	XM_012957573.2 , XM_012957573.3 , XM_012957574.2
Accession Version	XM_012957574.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2700bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1473177600000
Organism	Xenopus tropicalis(tropical clawed frog)
Product	HMG box transcription factor BBX isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030678.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012957574.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAAGGCA GTGTGAAAAA TAAGGACCAT TCTACTGACG GAGAAGGAGC TGGAAAACGA 
CCAAAGAGAA AATGCCTCCA ATGGCACCCA CTGCTTGCAA ACAAAATTCT TGACTTTTCT 
GAAGAAGATG AGGAAGAAGA GGATGAAGAA GATATTGATA AGGTCCAGCT TCTTGATGGT 
GATGGTCTAG AAAATGATGA AACAGAAGAC AATGATTCTA GTGAACAAAG AGCACGGAGG 
CCAATGAACG CTTTCCTCTT ATTTTGCAAA CGTCATCGTT CCCTAGTTCG TCAGGAACAT 
CCTCGTCTGG ATAATCGTGG TGCTACAAAA ATACTGGCAG ATTGGTGGGC CTATCTCGAC 
CCTAATGAGA AGCAGAAGTA CACAGATATG GCTAAAGAGT ACAAAGATGC ATTTATGAAA 
GCAAATCCTG GTTACAAGTG GTGTCCTACA ACTAATAAAC CCGTCAAAGC TCATTCACCT 
ACTGTAACTA CCAGGAAAAA ACTGTGGTCC TTTAGCTCAG AAACTGTCAA AGATGTTCCA 
AGCCCCAAGA AAGTCCCACA AGAAGATGAA ATGCCACAGC TTAATTTTAG TATTGCAGAT 
CCCACACAAA TGGGAGGACT GAGTATGCTG CTTCTTGCTG GTGAACATGC CTTGACTGCA 
CACGGGGATC ATGCTGTGAC TGAAAGAGTG GAACATTTAT TAAGTGATCA TGAGGTTTCT 
TCAGGTATTT GTCCTTTAAC TGAATCAGGG TTTCCCCAGG AAAATAGAGA CACTTCATTA 
TTTCAGTTTG ATGAGATATC TTCCAGCACA TCACATTCAG ATGACCCAGC ACCTGCTGAA 
CAAAGCGAGA AGTCAACACT GTTTCAGTTT GCCGAGATAT CTTCAGATAC TTCACAGACA 
CAGATTAATT GTGTGGATCC TGCAAAATGC TGTGGGAACT CTTCCCTTTT CCAATTAGCA 
GAGATATCTT CAAGTTCATC CCATTCTGAT TCATCTGCAT CAGCAAAGCA GTGTGGAACA 
TCAGCATTGT TTCAGCTTGC AGAGATGTGC GAGGCTTCTG AAGCTGTCAA AATAGAAATA 
TCCAGGAATG TAGATACAGA CGATTTGGTA AGAACGCCTG TGTCCATAAA GGAAGAAATA 
GAAAGCTCAT CAGAGGACCT GATTTCTAGA AACCTCAATG ATAGTTCACA TATCTGTGAG 
AAAGCAGAAA CTAATAACAT TAAAACTAAA AAGCACAAAG CACACACCCT AGAACCTATA 
GATGCTGAAC TTTTAGGACT TCTGAAACGG GAAAAATTAA AAAAGAAGAA GAAGAAAAAC 
AAATCAGACC ATGATGCTGC AGAGAAGGCA TCCCCAAAGA AAACCTGTAA AAAAAGGCAA 
TCATCGGAGT CTGATATTGA AAGTGTCATG TACACTATAG AAGCTGTCGC AAAAGGTGAT 
TGGGGCATTG AGCAAATTCC CATTTCTCCA AGCAAGAAGG CACGCATTTC TACTAGCCCC 
AAACACAGCA TTTTTGACTT AAAGTCAGCA AAGAAAAAAG TTAAATCAAA AGAAAAAAAA 
ATGCAGAAAG ATAAGCCCTC AGACAGTACC AAAGAGCTGT CAGCGCCAGA ACACATAATT 
ACTGCACCAA TCATCGATTT ACCTTCCGAG ACTCCTGAAA GTGTCAAATC AGAGCCACCT 
TCTCCCACTG AGGAGCTGCC TCCCATGTGC TTGCCAGAAA TGCTTAAAAC TGAGGACTTT 
AACAGCAATA AAAAGACAGA AACTTGTGGT TCAAGGAAAT CAGAACGGTC TTGTAAAGGA 
GCTCTTTATA AGACACTGGT GTCTGAAGGC ATGCTGACAT CACTGCGGGC CAATGTTGAC 
AGAGGTAAAA GAAACTCAGG AAAGGGCTGC TTATCAGACC ATGAAGGATG CTGGAATGAA 
GACAGCTGGG CATTCAATCA AACTGGAACC ACTGGGAGTA AAAAGCTGAA AAAAACTAAA 
CCAAAGGAAG AGTCGACACT TGGATTAGCA AAGCTGGAAG AAGAGTTTGA AAAGAAGTTT 
AACAGCCTTC CTCAGTACAG CCCTGTTACA TTTGATCGCA AAAATGCATC ACTTCCAAGG 
AAGAAGAAAA AGACTGGAAA TGGTATAGCT GAGCCACAAA AAGCCAATAA AGGTGTATTC 
CAGCCTCAGA AAAAGACCTT TTTTCACAAA ATTGTAAACA AGTACAAGCA CAAAAAAGAC 
AAACAAATTA TTTCGGATAA AGCCATAGCA GAAGAAAAAA ACAACAGGGA GAACTCACCC 
AGAGCCAATA ATTTTGTATC AGCTTCCAGC ATTCCAGAAT TTACAGCCAT ACAGGAGCCT 
CTGGTAGGAA GCCAAAAAAG GAAAGCAAGA AAAACAAAGA TAACCCACCT TGTTAGATCT 
ATGGATGGTC AGACATCATT AAAAGGACAG ATTTCAGATG AAAAACCACT GTCTAGTATT 
TGTGAAGAAG AGGGGTCCTG CAGAGGTGAA CTCTTAATCT CTAGAGAAAC TGAAGTGGAA 
CAGAATAATA GTATTGCCCA AGACATGCCT GCTGTGTCTG CATTTTTCAG TCTTGCTGCT 
CTGGCCGAGG TAGCTGCAAT GGAAAACGCT CACAGAAGTC CAAGAGGATC TCTTTCCCGA 
GATGGACAGA AGAAAGAAAT GTCCCAGGCA GCTGTGCTGA TTTCATGTGC TGACCAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012813028.1
CDS	968..3667
Translation	MKGSVKNKDHSTDGEGAGKRPKRKCLQWHPLLANKILDFSEEDEEEEDEEDIDKVQLLDGDGLENDETEDNDSSEQRARRPMNAFLLFCKRHRSLVRQEHPRLDNRGATKILADWWAYLDPNEKQKYTDMAKEYKDAFMKANPGYKWCPTTNKPVKAHSPTVTTRKKLWSFSSETVKDVPSPKKVPQEDEMPQLNFSIADPTQMGGLSMLLLAGEHALTAHGDHAVTERVEHLLSDHEVSSGICPLTESGFPQENRDTSLFQFDEISSSTSHSDDPAPAEQSEKSTLFQFAEISSDTSQTQINCVDPAKCCGNSSLFQLAEISSSSSHSDSSASAKQCGTSALFQLAEMCEASEAVKIEISRNVDTDDLVRTPVSIKEEIESSSEDLISRNLNDSSHICEKAETNNIKTKKHKAHTLEPIDAELLGLLKREKLKKKKKKNKSDHDAAEKASPKKTCKKRQSSESDIESVMYTIEAVAKGDWGIEQIPISPSKKARISTSPKHSIFDLKSAKKKVKSKEKKMQKDKPSDSTKELSAPEHIITAPIIDLPSETPESVKSEPPSPTEELPPMCLPEMLKTEDFNSNKKTETCGSRKSERSCKGALYKTLVSEGMLTSLRANVDRGKRNSGKGCLSDHEGCWNEDSWAFNQTGTTGSKKLKKTKPKEESTLGLAKLEEEFEKKFNSLPQYSPVTFDRKNASLPRKKKKTGNGIAEPQKANKGVFQPQKKTFFHKIVNKYKHKKDKQIISDKAIAEEKNNRENSPRANNFVSASSIPEFTAIQEPLVGSQKRKARKTKITHLVRSMDGQTSLKGQISDEKPLSSICEEEGSCRGELLISRETEVEQNNSIAQDMPAVSAFFSLAALAEVAAMENAHRSPRGSLSRDGQKKEMSQAAVLISCADQ

Target ORF information:

RefSeq Version	XM_012957574.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis bobby sox homolog (bbx), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012957574.2

ORF Insert Sequence:

ATGAAAGGCA GTGTGAAAAA TAAGGACCAT TCTACTGACG GAGAAGGAGC TGGAAAACGA 
CCAAAGAGAA AATGCCTCCA ATGGCACCCA CTGCTTGCAA ACAAAATTCT TGACTTTTCT 
GAAGAAGATG AGGAAGAAGA GGATGAAGAA GATATTGATA AGGTCCAGCT TCTTGATGGT 
GATGGTCTAG AAAATGATGA AACAGAAGAC AATGATTCTA GTGAACAAAG AGCACGGAGG 
CCAATGAACG CTTTCCTCTT ATTTTGCAAA CGTCATCGTT CCCTAGTTCG TCAGGAACAT 
CCTCGTCTGG ATAATCGTGG TGCTACAAAA ATACTGGCAG ATTGGTGGGC CTATCTCGAC 
CCTAATGAGA AGCAGAAGTA CACAGATATG GCTAAAGAGT ACAAAGATGC ATTTATGAAA 
GCAAATCCTG GTTACAAGTG GTGTCCTACA ACTAATAAAC CCGTCAAAGC TCATTCACCT 
ACTGTAACTA CCAGGAAAAA ACTGTGGTCC TTTAGCTCAG AAACTGTCAA AGATGTTCCA 
AGCCCCAAGA AAGTCCCACA AGAAGATGAA ATGCCACAGC TTAATTTTAG TATTGCAGAT 
CCCACACAAA TGGGAGGACT GAGTATGCTG CTTCTTGCTG GTGAACATGC CTTGACTGCA 
CACGGGGATC ATGCTGTGAC TGAAAGAGTG GAACATTTAT TAAGTGATCA TGAGGTTTCT 
TCAGGTATTT GTCCTTTAAC TGAATCAGGG TTTCCCCAGG AAAATAGAGA CACTTCATTA 
TTTCAGTTTG ATGAGATATC TTCCAGCACA TCACATTCAG ATGACCCAGC ACCTGCTGAA 
CAAAGCGAGA AGTCAACACT GTTTCAGTTT GCCGAGATAT CTTCAGATAC TTCACAGACA 
CAGATTAATT GTGTGGATCC TGCAAAATGC TGTGGGAACT CTTCCCTTTT CCAATTAGCA 
GAGATATCTT CAAGTTCATC CCATTCTGAT TCATCTGCAT CAGCAAAGCA GTGTGGAACA 
TCAGCATTGT TTCAGCTTGC AGAGATGTGC GAGGCTTCTG AAGCTGTCAA AATAGAAATA 
TCCAGGAATG TAGATACAGA CGATTTGGTA AGAACGCCTG TGTCCATAAA GGAAGAAATA 
GAAAGCTCAT CAGAGGACCT GATTTCTAGA AACCTCAATG ATAGTTCACA TATCTGTGAG 
AAAGCAGAAA CTAATAACAT TAAAACTAAA AAGCACAAAG CACACACCCT AGAACCTATA 
GATGCTGAAC TTTTAGGACT TCTGAAACGG GAAAAATTAA AAAAGAAGAA GAAGAAAAAC 
AAATCAGACC ATGATGCTGC AGAGAAGGCA TCCCCAAAGA AAACCTGTAA AAAAAGGCAA 
TCATCGGAGT CTGATATTGA AAGTGTCATG TACACTATAG AAGCTGTCGC AAAAGGTGAT 
TGGGGCATTG AGCAAATTCC CATTTCTCCA AGCAAGAAGG CACGCATTTC TACTAGCCCC 
AAACACAGCA TTTTTGACTT AAAGTCAGCA AAGAAAAAAG TTAAATCAAA AGAAAAAAAA 
ATGCAGAAAG ATAAGCCCTC AGACAGTACC AAAGAGCTGT CAGCGCCAGA ACACATAATT 
ACTGCACCAA TCATCGATTT ACCTTCCGAG ACTCCTGAAA GTGTCAAATC AGAGCCACCT 
TCTCCCACTG AGGAGCTGCC TCCCATGTGC TTGCCAGAAA TGCTTAAAAC TGAGGACTTT 
AACAGCAATA AAAAGACAGA AACTTGTGGT TCAAGGAAAT CAGAACGGTC TTGTAAAGGA 
GCTCTTTATA AGACACTGGT GTCTGAAGGC ATGCTGACAT CACTGCGGGC CAATGTTGAC 
AGAGGTAAAA GAAACTCAGG AAAGGGCTGC TTATCAGACC ATGAAGGATG CTGGAATGAA 
GACAGCTGGG CATTCAATCA AACTGGAACC ACTGGGAGTA AAAAGCTGAA AAAAACTAAA 
CCAAAGGAAG AGTCGACACT TGGATTAGCA AAGCTGGAAG AAGAGTTTGA AAAGAAGTTT 
AACAGCCTTC CTCAGTACAG CCCTGTTACA TTTGATCGCA AAAATGCATC ACTTCCAAGG 
AAGAAGAAAA AGACTGGAAA TGGTATAGCT GAGCCACAAA AAGCCAATAA AGGTGTATTC 
CAGCCTCAGA AAAAGACCTT TTTTCACAAA ATTGTAAACA AGTACAAGCA CAAAAAAGAC 
AAACAAATTA TTTCGGATAA AGCCATAGCA GAAGAAAAAA ACAACAGGGA GAACTCACCC 
AGAGCCAATA ATTTTGTATC AGCTTCCAGC ATTCCAGAAT TTACAGCCAT ACAGGAGCCT 
CTGGTAGGAA GCCAAAAAAG GAAAGCAAGA AAAACAAAGA TAACCCACCT TGTTAGATCT 
ATGGATGGTC AGACATCATT AAAAGGACAG ATTTCAGATG AAAAACCACT GTCTAGTATT 
TGTGAAGAAG AGGGGTCCTG CAGAGGTGAA CTCTTAATCT CTAGAGAAAC TGAAGTGGAA 
CAGAATAATA GTATTGCCCA AGACATGCCT GCTGTGTCTG CATTTTTCAG TCTTGCTGCT 
CTGGCCGAGG TAGCTGCAAT GGAAAACGCT CACAGAAGTC CAAGAGGATC TCTTTCCCGA 
GATGGACAGA AGAAAGAAAT GTCCCAGGCA GCTGTGCTGA TTTCATGTGC TGACCAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

bbx ( XM_018091607.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_017947096.1 Xenopus tropicalis bobby sox homolog (bbx), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OXa42777
Clone ID Related Accession (Same CDS sequence)	XM_018091607.1
Accession Version	XM_018091607.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2799bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1473177600000
Organism	Xenopus tropicalis(tropical clawed frog)
Product	HMG box transcription factor BBX isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030678.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACAGAGA GGAAGCCGAA AACACAGCGA ATTCGAATGA AGGAGCCATG GGGAATGAGC 
CGATCGGGGG TCACTCTCTG TAATGGAGAG CAGATCGGGT GTGCTGGCAG GAGGTCATTG 
TCACATGACA AAGAGATCAA AATAGAGTTA CCATTCTGTT CTTCCATTTG CCTTTCTTGG 
TGTATAGTCA TGAAAGGCAG TGTGAAAAAT AAGGACCATT CTACTGACGG AGAAGGAGCT 
GGAAAACGAC CAAAGAGAAA ATGCCTCCAA TGGCACCCAC TGCTTGCAAA CAAAATTCTT 
GACTTTTCTG AAGAAGATGA GGAAGAAGAG GATGAAGAAG ATATTGATAA GGTCCAGCTT 
CTTGATGGTG ATGGTCTAGA AAATGATGAA ACAGAAGACA ATGATTCTAG TGAACAAAGA 
GCACGGAGGC CAATGAACGC TTTCCTCTTA TTTTGCAAAC GTCATCGTTC CCTAGTTCGT 
CAGGAACATC CTCGTCTGGA TAATCGTGGT GCTACAAAAA TACTGGCAGA TTGGTGGGCC 
TATCTCGACC CTAATGAGAA GCAGAAGTAC ACAGATATGG CTAAAGAGTA CAAAGATGCA 
TTTATGAAAG CAAATCCTGG TTACAAGTGG TGTCCTACAA CTAATAAACC CGTCAAAGCT 
CATTCACCTA CTGTAACTAC CAGGAAAAAA CTGTGGTCCT TTAGCTCAGA AACTGTCAAA 
GATGTTCCAA GCCCCAAGAA AGTCCCACAA GAAGATGAAA TGCCACAGCT TAATTTTAGT 
ATTGCAGATC CCACACAAAT GGGAGGACTG AGTATGCTGC TTCTTGCTGG TGAACATGCC 
TTGACTGCAC ACGGGGATCA TGCTGTGACT GAAAGAGTGG AACATTTATT AAGTGATCAT 
GAGGTTTCTT CAGGTATTTG TCCTTTAACT GAATCAGGGT TTCCCCAGGA AAATAGAGAC 
ACTTCATTAT TTCAGTTTGA TGAGATATCT TCCAGCACAT CACATTCAGA TGACCCAGCA 
CCTGCTGAAC AAAGCGAGAA GTCAACACTG TTTCAGTTTG CCGAGATATC TTCAGATACT 
TCACAGACAC AGATTAATTG TGTGGATCCT GCAAAATGCT GTGGGAACTC TTCCCTTTTC 
CAATTAGCAG AGATATCTTC AAGTTCATCC CATTCTGATT CATCTGCATC AGCAAAGCAG 
TGTGGAACAT CAGCATTGTT TCAGCTTGCA GAGATGTGCG AGGCTTCTGA AGCTGTCAAA 
ATAGAAATAT CCAGGAATGT AGATACAGAC GATTTGGTAA GAACGCCTGT GTCCATAAAG 
GAAGAAATAG AAAGCTCATC AGAGGACCTG ATTTCTAGAA ACCTCAATGA TAGTTCACAT 
ATCTGTGAGA AAGCAGAAAC TAATAACATT AAAACTAAAA AGCACAAAGC ACACACCCTA 
GAACCTATAG ATGCTGAACT TTTAGGACTT CTGAAACGGG AAAAATTAAA AAAGAAGAAG 
AAGAAAAACA AATCAGACCA TGATGCTGCA GAGAAGGCAT CCCCAAAGAA AACCTGTAAA 
AAAAGGCAAT CATCGGAGTC TGATATTGAA AGTGTCATGT ACACTATAGA AGCTGTCGCA 
AAAGGTGATT GGGGCATTGA GCAAATTCCC ATTTCTCCAA GCAAGAAGGC ACGCATTTCT 
ACTAGCCCCA AACACAGCAT TTTTGACTTA AAGTCAGCAA AGAAAAAAGT TAAATCAAAA 
GAAAAAAAAA TGCAGAAAGA TAAGCCCTCA GACAGTACCA AAGAGCTGTC AGCGCCAGAA 
CACATAATTA CTGCACCAAT CATCGATTTA CCTTCCGAGA CTCCTGAAAG TGTCAAATCA 
GAGCCACCTT CTCCCACTGA GGAGCTGCCT CCCATGTGCT TGCCAGAAAT GCTTAAAACT 
GAGGACTTTA ACAGCAATAA AAAGACAGAA ACTTGTGGTT CAAGGAAATC AGAACGGTCT 
TGTAAAGGAG CTCTTTATAA GACACTGGTG TCTGAAGGCA TGCTGACATC ACTGCGGGCC 
AATGTTGACA GAGGTAAAAG AAACTCAGGA AAGGGCTGCT TATCAGACCA TGAAGGATGC 
TGGAATGAAG ACAGCTGGGC ATTCAATCAA ACTGGAACCA CTGGGAGTAA AAAGCTGAAA 
AAAACTAAAC CAAAGGAAGA GTCGACACTT GGATTAGCAA AGCTGGAAGA AGAGTTTGAA 
AAGAAGTTTA ACAGCCTTCC TCAGTACAGC CCTGTTACAT TTGATCGCAA AAATGCATCA 
CTTCCAAGGA AGAAGAAAAA GACTGGAAAT GGTATAGCTG AGCCACAAAA AGCCAATAAA 
GCCATAGCAG AAGAAAAAAA CAACAGGGAG AACTCACCCA GAGCCAATAA TTTTGTATCA 
GCTTCCAGCA TTCCAGAATT TACAGCCATA CAGGAGCCTC TGGTAGGAAG CCAAAAAAGG 
AAAGCAAGAA AAACAAAGAT AACCCACCTT GTTAGATCTA TGGATGGTCA GACATCATTA 
AAAGGACAGA TTTCAGATGA AAAACCACTG TCTAGTATTT GTGAAGAAGA GGGGTCCTGC 
AGAGGTGAAC TCTTAATCTC TAGAGAAACT GAAGTGGAAC AGAATAATAG TATTGCCCAA 
GACATGCCTG CTGTGTCTGC ATTTTTCAGT CTTGCTGCTC TGGCCGAGGT AGCTGCAATG 
GAAAACGCTC ACAGAAGTCC AAGAGGATCT CTTTCCCGAG ATGGACAGAA GAAAGAAATG 
TCCCAGGCAG CTGTGCTGAT TTCATGTGCT GACCAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017947096.1
CDS	1057..3855
Translation	MTERKPKTQRIRMKEPWGMSRSGVTLCNGEQIGCAGRRSLSHDKEIKIELPFCSSICLSWCIVMKGSVKNKDHSTDGEGAGKRPKRKCLQWHPLLANKILDFSEEDEEEEDEEDIDKVQLLDGDGLENDETEDNDSSEQRARRPMNAFLLFCKRHRSLVRQEHPRLDNRGATKILADWWAYLDPNEKQKYTDMAKEYKDAFMKANPGYKWCPTTNKPVKAHSPTVTTRKKLWSFSSETVKDVPSPKKVPQEDEMPQLNFSIADPTQMGGLSMLLLAGEHALTAHGDHAVTERVEHLLSDHEVSSGICPLTESGFPQENRDTSLFQFDEISSSTSHSDDPAPAEQSEKSTLFQFAEISSDTSQTQINCVDPAKCCGNSSLFQLAEISSSSSHSDSSASAKQCGTSALFQLAEMCEASEAVKIEISRNVDTDDLVRTPVSIKEEIESSSEDLISRNLNDSSHICEKAETNNIKTKKHKAHTLEPIDAELLGLLKREKLKKKKKKNKSDHDAAEKASPKKTCKKRQSSESDIESVMYTIEAVAKGDWGIEQIPISPSKKARISTSPKHSIFDLKSAKKKVKSKEKKMQKDKPSDSTKELSAPEHIITAPIIDLPSETPESVKSEPPSPTEELPPMCLPEMLKTEDFNSNKKTETCGSRKSERSCKGALYKTLVSEGMLTSLRANVDRGKRNSGKGCLSDHEGCWNEDSWAFNQTGTTGSKKLKKTKPKEESTLGLAKLEEEFEKKFNSLPQYSPVTFDRKNASLPRKKKKTGNGIAEPQKANKAIAEEKNNRENSPRANNFVSASSIPEFTAIQEPLVGSQKRKARKTKITHLVRSMDGQTSLKGQISDEKPLSSICEEEGSCRGELLISRETEVEQNNSIAQDMPAVSAFFSLAALAEVAAMENAHRSPRGSLSRDGQKKEMSQAAVLISCADQ

Target ORF information:

RefSeq Version	XM_018091607.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis bobby sox homolog (bbx), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_018091607.1

ORF Insert Sequence:

ATGACAGAGA GGAAGCCGAA AACACAGCGA ATTCGAATGA AGGAGCCATG GGGAATGAGC 
CGATCGGGGG TCACTCTCTG TAATGGAGAG CAGATCGGGT GTGCTGGCAG GAGGTCATTG 
TCACATGACA AAGAGATCAA AATAGAGTTA CCATTCTGTT CTTCCATTTG CCTTTCTTGG 
TGTATAGTCA TGAAAGGCAG TGTGAAAAAT AAGGACCATT CTACTGACGG AGAAGGAGCT 
GGAAAACGAC CAAAGAGAAA ATGCCTCCAA TGGCACCCAC TGCTTGCAAA CAAAATTCTT 
GACTTTTCTG AAGAAGATGA GGAAGAAGAG GATGAAGAAG ATATTGATAA GGTCCAGCTT 
CTTGATGGTG ATGGTCTAGA AAATGATGAA ACAGAAGACA ATGATTCTAG TGAACAAAGA 
GCACGGAGGC CAATGAACGC TTTCCTCTTA TTTTGCAAAC GTCATCGTTC CCTAGTTCGT 
CAGGAACATC CTCGTCTGGA TAATCGTGGT GCTACAAAAA TACTGGCAGA TTGGTGGGCC 
TATCTCGACC CTAATGAGAA GCAGAAGTAC ACAGATATGG CTAAAGAGTA CAAAGATGCA 
TTTATGAAAG CAAATCCTGG TTACAAGTGG TGTCCTACAA CTAATAAACC CGTCAAAGCT 
CATTCACCTA CTGTAACTAC CAGGAAAAAA CTGTGGTCCT TTAGCTCAGA AACTGTCAAA 
GATGTTCCAA GCCCCAAGAA AGTCCCACAA GAAGATGAAA TGCCACAGCT TAATTTTAGT 
ATTGCAGATC CCACACAAAT GGGAGGACTG AGTATGCTGC TTCTTGCTGG TGAACATGCC 
TTGACTGCAC ACGGGGATCA TGCTGTGACT GAAAGAGTGG AACATTTATT AAGTGATCAT 
GAGGTTTCTT CAGGTATTTG TCCTTTAACT GAATCAGGGT TTCCCCAGGA AAATAGAGAC 
ACTTCATTAT TTCAGTTTGA TGAGATATCT TCCAGCACAT CACATTCAGA TGACCCAGCA 
CCTGCTGAAC AAAGCGAGAA GTCAACACTG TTTCAGTTTG CCGAGATATC TTCAGATACT 
TCACAGACAC AGATTAATTG TGTGGATCCT GCAAAATGCT GTGGGAACTC TTCCCTTTTC 
CAATTAGCAG AGATATCTTC AAGTTCATCC CATTCTGATT CATCTGCATC AGCAAAGCAG 
TGTGGAACAT CAGCATTGTT TCAGCTTGCA GAGATGTGCG AGGCTTCTGA AGCTGTCAAA 
ATAGAAATAT CCAGGAATGT AGATACAGAC GATTTGGTAA GAACGCCTGT GTCCATAAAG 
GAAGAAATAG AAAGCTCATC AGAGGACCTG ATTTCTAGAA ACCTCAATGA TAGTTCACAT 
ATCTGTGAGA AAGCAGAAAC TAATAACATT AAAACTAAAA AGCACAAAGC ACACACCCTA 
GAACCTATAG ATGCTGAACT TTTAGGACTT CTGAAACGGG AAAAATTAAA AAAGAAGAAG 
AAGAAAAACA AATCAGACCA TGATGCTGCA GAGAAGGCAT CCCCAAAGAA AACCTGTAAA 
AAAAGGCAAT CATCGGAGTC TGATATTGAA AGTGTCATGT ACACTATAGA AGCTGTCGCA 
AAAGGTGATT GGGGCATTGA GCAAATTCCC ATTTCTCCAA GCAAGAAGGC ACGCATTTCT 
ACTAGCCCCA AACACAGCAT TTTTGACTTA AAGTCAGCAA AGAAAAAAGT TAAATCAAAA 
GAAAAAAAAA TGCAGAAAGA TAAGCCCTCA GACAGTACCA AAGAGCTGTC AGCGCCAGAA 
CACATAATTA CTGCACCAAT CATCGATTTA CCTTCCGAGA CTCCTGAAAG TGTCAAATCA 
GAGCCACCTT CTCCCACTGA GGAGCTGCCT CCCATGTGCT TGCCAGAAAT GCTTAAAACT 
GAGGACTTTA ACAGCAATAA AAAGACAGAA ACTTGTGGTT CAAGGAAATC AGAACGGTCT 
TGTAAAGGAG CTCTTTATAA GACACTGGTG TCTGAAGGCA TGCTGACATC ACTGCGGGCC 
AATGTTGACA GAGGTAAAAG AAACTCAGGA AAGGGCTGCT TATCAGACCA TGAAGGATGC 
TGGAATGAAG ACAGCTGGGC ATTCAATCAA ACTGGAACCA CTGGGAGTAA AAAGCTGAAA 
AAAACTAAAC CAAAGGAAGA GTCGACACTT GGATTAGCAA AGCTGGAAGA AGAGTTTGAA 
AAGAAGTTTA ACAGCCTTCC TCAGTACAGC CCTGTTACAT TTGATCGCAA AAATGCATCA 
CTTCCAAGGA AGAAGAAAAA GACTGGAAAT GGTATAGCTG AGCCACAAAA AGCCAATAAA 
GCCATAGCAG AAGAAAAAAA CAACAGGGAG AACTCACCCA GAGCCAATAA TTTTGTATCA 
GCTTCCAGCA TTCCAGAATT TACAGCCATA CAGGAGCCTC TGGTAGGAAG CCAAAAAAGG 
AAAGCAAGAA AAACAAAGAT AACCCACCTT GTTAGATCTA TGGATGGTCA GACATCATTA 
AAAGGACAGA TTTCAGATGA AAAACCACTG TCTAGTATTT GTGAAGAAGA GGGGTCCTGC 
AGAGGTGAAC TCTTAATCTC TAGAGAAACT GAAGTGGAAC AGAATAATAG TATTGCCCAA 
GACATGCCTG CTGTGTCTGC ATTTTTCAGT CTTGCTGCTC TGGCCGAGGT AGCTGCAATG 
GAAAACGCTC ACAGAAGTCC AAGAGGATCT CTTTCCCGAG ATGGACAGAA GAAAGAAATG 
TCCCAGGCAG CTGTGCTGAT TTCATGTGCT GACCAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

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bbx cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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