dgkh cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

Gene
Clones
gRNAs

Summary

Gene Symbol	dgkh
Entrez Gene ID	100486581
Full Name	diacylglycerol kinase, eta
General protein information	Preferred Names diacylglycerol kinase, eta Names diacylglycerol kinase eta
Gene Type	protein-coding
Organism	Xenopus tropicalis(tropical clawed frog)

mRNA and Protein(s)

mRNA	Protein	Name
XM_012957746.3	XP_012813200.1	diacylglycerol kinase eta isoform X3
XM_004911834.4	XP_004911891.1	diacylglycerol kinase eta isoform X2
XM_012957748.2	XP_012813202.1	diacylglycerol kinase eta isoform X4
XM_012957745.2	XP_012813199.1	diacylglycerol kinase eta isoform X1
XM_012957746.2	XP_012813200.1	diacylglycerol kinase eta isoform X3
XM_012957748.3	XP_012813202.1	diacylglycerol kinase eta isoform X4
XM_012957745.3	XP_012813199.1	diacylglycerol kinase eta isoform X1
XM_004911834.3	XP_004911891.1	diacylglycerol kinase eta isoform X2

Pathways from BioSystems

Homologies

Xenopus tropicalis (tropical clawed frog)	dgkh	XP_004911891.1
Canis lupus familiaris (dog)	DGKH	XP_005633986.1
Mus musculus (house mouse)	Dgkh	NP_001240695.1
Danio rerio (zebrafish)	dgkh	XP_005168036.1
Macaca mulatta (Rhesus monkey)	DGKH	XP_002800800.1
Bos taurus (cattle)	DGKH	XP_002691870.2
Rattus norvegicus (Norway rat)	Dgkh	XP_002728322.3
Drosophila melanogaster (fruit fly)	CG34384	NP_001097704.1
Pan troglodytes (chimpanzee)	DGKH	XP_001151535.2
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP002226	XP_307958.5
Homo sapiens (human)	DGKH	NP_821077.1
Gallus gallus (chicken)	DGKH	XP_001232791.2

Gene Ontology

The following dgkh gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the dgkh cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OXa05943	XM_004911834.3 Latest version!	Xenopus tropicalis diacylglycerol kinase, eta (dgkh), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OXa33656	XM_012957745.2 Latest version!	Xenopus tropicalis diacylglycerol kinase, eta (dgkh), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OXa33657	XM_012957746.2 Latest version!	Xenopus tropicalis diacylglycerol kinase, eta (dgkh), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OXa33658	XM_012957748.2 Latest version!	Xenopus tropicalis diacylglycerol kinase, eta (dgkh), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OXa52617	XM_012957745.3 Latest version!	Xenopus tropicalis diacylglycerol kinase, eta (dgkh), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OXa52618	XM_004911834.4 Latest version!	Xenopus tropicalis diacylglycerol kinase, eta (dgkh), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OXa52619	XM_012957746.3 Latest version!	Xenopus tropicalis diacylglycerol kinase, eta (dgkh), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OXa52620	XM_012957748.3 Latest version!	Xenopus tropicalis diacylglycerol kinase, eta (dgkh), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

dgkh ( XM_004911834.3 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_004911891.1 Xenopus tropicalis diacylglycerol kinase, eta (dgkh), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OXa05943
Clone ID Related Accession (Same CDS sequence)	XM_004911834.3
Accession Version	XM_004911834.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3711bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1473177600000
Organism	Xenopus tropicalis(tropical clawed frog)
Product	diacylglycerol kinase eta isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030678.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_004911834.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGGAGA CTGCCTGCCC CTCGTACAGC CGCCGGAGCC CAGTTTGGGA AGAGATGCAG 
GAGTGCGACA AGATGTCGGG TTCAATGAGG GAGCCTGCGG TGGCCCCTGG AGCTCCTGCG 
GGAGATGAGT CCTCTGACAG CGAGGGGGAG CATGAGGGGC CGCAGAAACT CATTCGCAAA 
GTGTCCACCT CTGGGCAACT CCGCAGCAAG ACAAGTATCA AAGAGGGATT ATTACTGAAG 
CAAACCAGTT CATTTCAGAG ATGGAAAAAA CGCTATTTTA AGCTACGTGG CAGAACCTTG 
TACTATGCCA AGGATTCAAA GTCTCTAATA TTTGATGAAG TTGACCTGTC AGATGCCAGT 
GTAGCTGAAT CAAGCACAAA AAATGTCAAC AACAGCTTCA CGATAATCAC CCCTTTCAGA 
AGGCTTATAT TGTGTGCTGA GAACAGAAAA GAAATGGAGG ATTGGATCAG TTCACTAAAG 
TCTGTCCAGT CAAGAGAGCA TTATGAGACC GCACAGTTTA ATGTGGAACA TTTCTCAGGG 
ATGCATAACT GGTATGCATG TTCCCATGCC CGACCCACCT TCTGTAATGT GTGCAGAGAA 
AGCCTTTCTG GGGTTACCTC ACATGGCCTT TCTTGTGAAG TCTGCAAATT CAAAGCTCAT 
AAAAGATGTG CAGTTAGAGC AACAAACAAC TGCAAGTGGA CCACCCTGGC TTCAATCGGC 
AAAGATATTA TTGAGGATGA GGATGGTATA GCAATGCCTC ACCAGTGGCT AGAGGGTAAT 
CTGCCTGTTA GTGCCAAATG TGCTGTTTGT GACAAAACCT GTGGCAGCGT ATTACGTCTG 
CAGGACTGGA GGTGCCTTTG GTGTAAAGCA ATGGTGCACA CAGCATGCAA GGACCAGTAT 
CCTCGAAAGT GTCCATTGGG CCAGTGCAAA GTCTCCATCA TACCTCCAAC GGCACTCAAC 
AGTATCGACT CTGATGGGTT TTGGAAAGCA ACATGCCCAC CTTCCTGTGC CAGCCCACTT 
CTAGTTTTTG TCAATTCCAA AAGCGGAGAC AATCAGGGGG TCAAGTTTCT ACGGAGGTTC 
AAACAGTTAC TTAATCCTGC TCAGGTGTTC GACTTAATGA ATGGAGGACC CCATCTAGGT 
TTACGTTTGT TTCAGAAGTT TGACAATTTC CGGATCCTTG TTTGTGGTGG AGATGGAAGT 
GTTGGTTGGG TTTTATCGGA GATTGATAAG CTAAGTTTAC ATAAACAGTG TCAACTGGGC 
GTACTTCCAC TGGGTACTGG AAATGACTTG GCTCGTGTGC TAGGTTGGGG TGCTTCTTGT 
GATGATGATA CTCAGCTGCC TCAAATTCTG GAAAAGTTGG AACGTGCTAG CACAAAAATG 
CTGGATAGAT GGAGTATTAT GTCATATGAA CTTAAATTGC CAACAAAGCC TTCTATATCT 
CCTTTATCTC CAGAGGAGGA ATCTGAAGAG TGTCAATTTC AGATATCTGC ATATGAGGAT 
TCAGTGGCTA CTCATCTTGC AAAGATTCTA AATTCAGACA AGCATTCAGT AGTGATTTCC 
TCTGCTAAGG TTTTATGTGA AACTGTAAAG GACTTTGTTG CAAAAGTCGG GAAGGCTTAT 
GAGAAACCAA TGGAAAATGC AGAGGAAGCT GAATCAATGG CTGTAAAGTG TTCAACTCTG 
AATGAAAAAT TGGACCTGCT GCTTCAGACT CTGCATGCAG AATCTCAGGC TGCTCCATTG 
CTGGCACATT CCACCCCCCC TATTGTGGAA GAAGAAGTTG AGGAAGAGTT TTCCAGTGAA 
GAATCCCTGT CTGAAAGTAG AGAACAGTTA GCTGACAGCA TACCAAAGTC TTCCAGCCAA 
AGGCTCTTCA AACCAAGAGA GCAACTCATG CTGCGGGCTA ACAGCCTGAA AAAAGCTGTC 
AGGCAGATAA TAGAGCAAGC TGAGAAAGTG GTAGATGAGC AGAATGCACA CACTGAGGAA 
CAAGAAAACC AGTCTCCTGA GGAATACAGA AAGGAGTTGG AGGAATCTAA AGAGGAAGAA 
AAAGATGAAG ACACAAAAGA ATCTGAATTT CAGGAGTCTG GCAAGGTTCC CCCACGGTCA 
CCAGACAGAC AGACAAGCCG CAGTACATAT ACTCACACAG GCTCATTCTC AGGGCCACCC 
TTGGCATCAA GCAAGGAAAA CCTACCTGTG TTGAACACTA GAATAATCTG TCCAGGTCTG 
AGGGCAGGGT TGGCGGCCTC CATCGCTGGG AGTTCTATCA TCAATAAAAT GTTACTGGCA 
AACATAGATC CACTGGGGGC TACTCCATTT ATTGACCCTG ATCCAGATTC ACTGGAAGGC 
TATTCAGAAA AATGTGTAAT GAACAACTAT TTTGGAATTG GCCTGGATGC AAAGATATCC 
TTAGAATTTA ACAACAAGCG AGAAGAACAC CCTGAAAAAT GCAGAAGCCG AACTAAAAAT 
ATGATGTGGT ATGGTGTGCT TGGCACCAAG GAACTTCTGC AGCGAACATA CAAAAACTTA 
GAGCAGAAAG TCCAGCTTGA GTGTGATGGG CAGTACATCC CTCTTCCCAG CCTTCAGGGT 
ATTGCTGTGC TGAACATTCC AAGCTATGCT GGCGGCACTA ACTTCTGGGG TGGAACCAAG 
GAAGATGATA TCTTTGGTGC GCCGTCATTT GATGATAAAA TCTTGGAGGT GGTAGCAGTT 
TTTGGAAGTA TGCAGATGGC AGTATCTAGG GTGATAAAGC TGCAACATCA TAGAATAGCA 
CAGTGCCGGT CAGTGAAGAT CACCATACTT GGAGAAGAGG GAGTCCCTGT TCAGGTGGAT 
GGAGAGGCTT GGATTCAGCC ACCTGGCGTT ATTAAAATTG TACATAAAAA CCGAGCTCAG 
ATGCTGACTA GGGACAGGGC TTTTGAGAAC ACCCTGAAGT CCTGGGAAGA TAAGCAGAAG 
TACGATTCCT GTAAGCTTCC CCTTCGCTCT CATCTCTATT CTCAACAAGC TGTTGATTTG 
GCTTCAGAAG AAGAAGTCAC ACTAATCCAT CTTTGCTCGA AAGCTTCAGA GGAGCTTATT 
ACAAGAATAT GCGAAGCTGC AAAAATCCAT AGCTTGCTGG AACAAGAACT GGCTCATGCG 
GTGAATGCCT GCTCTCATGC ACTAAATAAA GCCAATCCCC GGTTTCCCGA GAGCCTAACA 
AGAAACACTG CCATTGAGGT AGCAGTAACT GCAAAAGCTT TGCACAATGA AACAGAATCT 
CTCTTGGTTG GAAGAGTGCC CCTGCAGTTG GAATCCCATC ATGAAGAACT TTTATCCAAT 
GCCCTTCACA GTGTAGAAGT TGAGCTAACA AAACTGGCTG AGATTCCTTG GCTGTACTTT 
GTCTTACAGC CAGATGAGGA GGATCCTCCT CTGGAATACT CTAAAAGAAA CAGCAGAAGC 
ACAGTATTCC GAATAGTGCC AAAGTTTAAA AGGGAGAAGG CCTTAAAGAA TAAGCCCAGC 
ATTCAGCCTG TTCAAAAATG GGGCACAGAT GAAGTGGCAG TTTGGCTGGA TCTTCTCAGT 
TTGGGAGAGT ACAGAGAAAT CTTCATCCGT CATGATATCC GTGGCTCAGA GCTATTGCAC 
CTGGAGAGGC GGGACCTTAA GGATCTGGGG ATATTGAAAG TGGGTCATAT GAAGCGAATT 
CTACAGGGAA TTAAAGAGCT CAGCAAAAAC TCCTCTTTAT CGGACATGTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004911891.1
CDS	2641..6351
Translation	MMETACPSYSRRSPVWEEMQECDKMSGSMREPAVAPGAPAGDESSDSEGEHEGPQKLIRKVSTSGQLRSKTSIKEGLLLKQTSSFQRWKKRYFKLRGRTLYYAKDSKSLIFDEVDLSDASVAESSTKNVNNSFTIITPFRRLILCAENRKEMEDWISSLKSVQSREHYETAQFNVEHFSGMHNWYACSHARPTFCNVCRESLSGVTSHGLSCEVCKFKAHKRCAVRATNNCKWTTLASIGKDIIEDEDGIAMPHQWLEGNLPVSAKCAVCDKTCGSVLRLQDWRCLWCKAMVHTACKDQYPRKCPLGQCKVSIIPPTALNSIDSDGFWKATCPPSCASPLLVFVNSKSGDNQGVKFLRRFKQLLNPAQVFDLMNGGPHLGLRLFQKFDNFRILVCGGDGSVGWVLSEIDKLSLHKQCQLGVLPLGTGNDLARVLGWGASCDDDTQLPQILEKLERASTKMLDRWSIMSYELKLPTKPSISPLSPEEESEECQFQISAYEDSVATHLAKILNSDKHSVVISSAKVLCETVKDFVAKVGKAYEKPMENAEEAESMAVKCSTLNEKLDLLLQTLHAESQAAPLLAHSTPPIVEEEVEEEFSSEESLSESREQLADSIPKSSSQRLFKPREQLMLRANSLKKAVRQIIEQAEKVVDEQNAHTEEQENQSPEEYRKELEESKEEEKDEDTKESEFQESGKVPPRSPDRQTSRSTYTHTGSFSGPPLASSKENLPVLNTRIICPGLRAGLAASIAGSSIINKMLLANIDPLGATPFIDPDPDSLEGYSEKCVMNNYFGIGLDAKISLEFNNKREEHPEKCRSRTKNMMWYGVLGTKELLQRTYKNLEQKVQLECDGQYIPLPSLQGIAVLNIPSYAGGTNFWGGTKEDDIFGAPSFDDKILEVVAVFGSMQMAVSRVIKLQHHRIAQCRSVKITILGEEGVPVQVDGEAWIQPPGVIKIVHKNRAQMLTRDRAFENTLKSWEDKQKYDSCKLPLRSHLYSQQAVDLASEEEVTLIHLCSKASEELITRICEAAKIHSLLEQELAHAVNACSHALNKANPRFPESLTRNTAIEVAVTAKALHNETESLLVGRVPLQLESHHEELLSNALHSVEVELTKLAEIPWLYFVLQPDEEDPPLEYSKRNSRSTVFRIVPKFKREKALKNKPSIQPVQKWGTDEVAVWLDLLSLGEYREIFIRHDIRGSELLHLERRDLKDLGILKVGHMKRILQGIKELSKNSSLSDM

Target ORF information:

RefSeq Version	XM_004911834.3
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis diacylglycerol kinase, eta (dgkh), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004911834.3

ORF Insert Sequence:

ATGATGGAGA CTGCCTGCCC CTCGTACAGC CGCCGGAGCC CAGTTTGGGA AGAGATGCAG 
GAGTGCGACA AGATGTCGGG TTCAATGAGG GAGCCTGCGG TGGCCCCTGG AGCTCCTGCG 
GGAGATGAGT CCTCTGACAG CGAGGGGGAG CATGAGGGGC CGCAGAAACT CATTCGCAAA 
GTGTCCACCT CTGGGCAACT CCGCAGCAAG ACAAGTATCA AAGAGGGATT ATTACTGAAG 
CAAACCAGTT CATTTCAGAG ATGGAAAAAA CGCTATTTTA AGCTACGTGG CAGAACCTTG 
TACTATGCCA AGGATTCAAA GTCTCTAATA TTTGATGAAG TTGACCTGTC AGATGCCAGT 
GTAGCTGAAT CAAGCACAAA AAATGTCAAC AACAGCTTCA CGATAATCAC CCCTTTCAGA 
AGGCTTATAT TGTGTGCTGA GAACAGAAAA GAAATGGAGG ATTGGATCAG TTCACTAAAG 
TCTGTCCAGT CAAGAGAGCA TTATGAGACC GCACAGTTTA ATGTGGAACA TTTCTCAGGG 
ATGCATAACT GGTATGCATG TTCCCATGCC CGACCCACCT TCTGTAATGT GTGCAGAGAA 
AGCCTTTCTG GGGTTACCTC ACATGGCCTT TCTTGTGAAG TCTGCAAATT CAAAGCTCAT 
AAAAGATGTG CAGTTAGAGC AACAAACAAC TGCAAGTGGA CCACCCTGGC TTCAATCGGC 
AAAGATATTA TTGAGGATGA GGATGGTATA GCAATGCCTC ACCAGTGGCT AGAGGGTAAT 
CTGCCTGTTA GTGCCAAATG TGCTGTTTGT GACAAAACCT GTGGCAGCGT ATTACGTCTG 
CAGGACTGGA GGTGCCTTTG GTGTAAAGCA ATGGTGCACA CAGCATGCAA GGACCAGTAT 
CCTCGAAAGT GTCCATTGGG CCAGTGCAAA GTCTCCATCA TACCTCCAAC GGCACTCAAC 
AGTATCGACT CTGATGGGTT TTGGAAAGCA ACATGCCCAC CTTCCTGTGC CAGCCCACTT 
CTAGTTTTTG TCAATTCCAA AAGCGGAGAC AATCAGGGGG TCAAGTTTCT ACGGAGGTTC 
AAACAGTTAC TTAATCCTGC TCAGGTGTTC GACTTAATGA ATGGAGGACC CCATCTAGGT 
TTACGTTTGT TTCAGAAGTT TGACAATTTC CGGATCCTTG TTTGTGGTGG AGATGGAAGT 
GTTGGTTGGG TTTTATCGGA GATTGATAAG CTAAGTTTAC ATAAACAGTG TCAACTGGGC 
GTACTTCCAC TGGGTACTGG AAATGACTTG GCTCGTGTGC TAGGTTGGGG TGCTTCTTGT 
GATGATGATA CTCAGCTGCC TCAAATTCTG GAAAAGTTGG AACGTGCTAG CACAAAAATG 
CTGGATAGAT GGAGTATTAT GTCATATGAA CTTAAATTGC CAACAAAGCC TTCTATATCT 
CCTTTATCTC CAGAGGAGGA ATCTGAAGAG TGTCAATTTC AGATATCTGC ATATGAGGAT 
TCAGTGGCTA CTCATCTTGC AAAGATTCTA AATTCAGACA AGCATTCAGT AGTGATTTCC 
TCTGCTAAGG TTTTATGTGA AACTGTAAAG GACTTTGTTG CAAAAGTCGG GAAGGCTTAT 
GAGAAACCAA TGGAAAATGC AGAGGAAGCT GAATCAATGG CTGTAAAGTG TTCAACTCTG 
AATGAAAAAT TGGACCTGCT GCTTCAGACT CTGCATGCAG AATCTCAGGC TGCTCCATTG 
CTGGCACATT CCACCCCCCC TATTGTGGAA GAAGAAGTTG AGGAAGAGTT TTCCAGTGAA 
GAATCCCTGT CTGAAAGTAG AGAACAGTTA GCTGACAGCA TACCAAAGTC TTCCAGCCAA 
AGGCTCTTCA AACCAAGAGA GCAACTCATG CTGCGGGCTA ACAGCCTGAA AAAAGCTGTC 
AGGCAGATAA TAGAGCAAGC TGAGAAAGTG GTAGATGAGC AGAATGCACA CACTGAGGAA 
CAAGAAAACC AGTCTCCTGA GGAATACAGA AAGGAGTTGG AGGAATCTAA AGAGGAAGAA 
AAAGATGAAG ACACAAAAGA ATCTGAATTT CAGGAGTCTG GCAAGGTTCC CCCACGGTCA 
CCAGACAGAC AGACAAGCCG CAGTACATAT ACTCACACAG GCTCATTCTC AGGGCCACCC 
TTGGCATCAA GCAAGGAAAA CCTACCTGTG TTGAACACTA GAATAATCTG TCCAGGTCTG 
AGGGCAGGGT TGGCGGCCTC CATCGCTGGG AGTTCTATCA TCAATAAAAT GTTACTGGCA 
AACATAGATC CACTGGGGGC TACTCCATTT ATTGACCCTG ATCCAGATTC ACTGGAAGGC 
TATTCAGAAA AATGTGTAAT GAACAACTAT TTTGGAATTG GCCTGGATGC AAAGATATCC 
TTAGAATTTA ACAACAAGCG AGAAGAACAC CCTGAAAAAT GCAGAAGCCG AACTAAAAAT 
ATGATGTGGT ATGGTGTGCT TGGCACCAAG GAACTTCTGC AGCGAACATA CAAAAACTTA 
GAGCAGAAAG TCCAGCTTGA GTGTGATGGG CAGTACATCC CTCTTCCCAG CCTTCAGGGT 
ATTGCTGTGC TGAACATTCC AAGCTATGCT GGCGGCACTA ACTTCTGGGG TGGAACCAAG 
GAAGATGATA TCTTTGGTGC GCCGTCATTT GATGATAAAA TCTTGGAGGT GGTAGCAGTT 
TTTGGAAGTA TGCAGATGGC AGTATCTAGG GTGATAAAGC TGCAACATCA TAGAATAGCA 
CAGTGCCGGT CAGTGAAGAT CACCATACTT GGAGAAGAGG GAGTCCCTGT TCAGGTGGAT 
GGAGAGGCTT GGATTCAGCC ACCTGGCGTT ATTAAAATTG TACATAAAAA CCGAGCTCAG 
ATGCTGACTA GGGACAGGGC TTTTGAGAAC ACCCTGAAGT CCTGGGAAGA TAAGCAGAAG 
TACGATTCCT GTAAGCTTCC CCTTCGCTCT CATCTCTATT CTCAACAAGC TGTTGATTTG 
GCTTCAGAAG AAGAAGTCAC ACTAATCCAT CTTTGCTCGA AAGCTTCAGA GGAGCTTATT 
ACAAGAATAT GCGAAGCTGC AAAAATCCAT AGCTTGCTGG AACAAGAACT GGCTCATGCG 
GTGAATGCCT GCTCTCATGC ACTAAATAAA GCCAATCCCC GGTTTCCCGA GAGCCTAACA 
AGAAACACTG CCATTGAGGT AGCAGTAACT GCAAAAGCTT TGCACAATGA AACAGAATCT 
CTCTTGGTTG GAAGAGTGCC CCTGCAGTTG GAATCCCATC ATGAAGAACT TTTATCCAAT 
GCCCTTCACA GTGTAGAAGT TGAGCTAACA AAACTGGCTG AGATTCCTTG GCTGTACTTT 
GTCTTACAGC CAGATGAGGA GGATCCTCCT CTGGAATACT CTAAAAGAAA CAGCAGAAGC 
ACAGTATTCC GAATAGTGCC AAAGTTTAAA AGGGAGAAGG CCTTAAAGAA TAAGCCCAGC 
ATTCAGCCTG TTCAAAAATG GGGCACAGAT GAAGTGGCAG TTTGGCTGGA TCTTCTCAGT 
TTGGGAGAGT ACAGAGAAAT CTTCATCCGT CATGATATCC GTGGCTCAGA GCTATTGCAC 
CTGGAGAGGC GGGACCTTAA GGATCTGGGG ATATTGAAAG TGGGTCATAT GAAGCGAATT 
CTACAGGGAA TTAAAGAGCT CAGCAAAAAC TCCTCTTTAT CGGACATGTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

dgkh ( XM_012957745.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012813199.1 Xenopus tropicalis diacylglycerol kinase, eta (dgkh), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OXa33656
Clone ID Related Accession (Same CDS sequence)	XM_012957745.2
Accession Version	XM_012957745.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3741bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1473177600000
Organism	Xenopus tropicalis(tropical clawed frog)
Product	diacylglycerol kinase eta isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030678.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012957745.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGCCA GGCGCTTCGC TGTGCCCCCG GCCGGTAAAA GGGGTGTAGG GAACCTGGCT 
GACACGGACT GCTTTGTTTT TTCCCAGCAG GAGTGCGACA AGATGTCGGG TTCAATGAGG 
GAGCCTGCGG TGGCCCCTGG AGCTCCTGCG GGAGATGAGT CCTCTGACAG CGAGGGGGAG 
CATGAGGGGC CGCAGAAACT CATTCGCAAA GTGTCCACCT CTGGGCAACT CCGCAGCAAG 
ACAAGTATCA AAGAGGGATT ATTACTGAAG CAAACCAGTT CATTTCAGAG ATGGAAAAAA 
CGCTATTTTA AGCTACGTGG CAGAACCTTG TACTATGCCA AGGATTCAAA GTCTCTAATA 
TTTGATGAAG TTGACCTGTC AGATGCCAGT GTAGCTGAAT CAAGCACAAA AAATGTCAAC 
AACAGCTTCA CGATAATCAC CCCTTTCAGA AGGCTTATAT TGTGTGCTGA GAACAGAAAA 
GAAATGGAGG ATTGGATCAG TTCACTAAAG TCTGTCCAGT CAAGAGAGCA TTATGAGACC 
GCACAGTTTA ATGTGGAACA TTTCTCAGGG ATGCATAACT GGTATGCATG TTCCCATGCC 
CGACCCACCT TCTGTAATGT GTGCAGAGAA AGCCTTTCTG GGGTTACCTC ACATGGCCTT 
TCTTGTGAAG TCTGCAAATT CAAAGCTCAT AAAAGATGTG CAGTTAGAGC AACAAACAAC 
TGCAAGTGGA CCACCCTGGC TTCAATCGGC AAAGATATTA TTGAGGATGA GGATGGTATA 
GCAATGCCTC ACCAGTGGCT AGAGGGTAAT CTGCCTGTTA GTGCCAAATG TGCTGTTTGT 
GACAAAACCT GTGGCAGCGT ATTACGTCTG CAGGACTGGA GGTGCCTTTG GTGTAAAGCA 
ATGGTGCACA CAGCATGCAA GGACCAGTAT CCTCGAAAGT GTCCATTGGG CCAGTGCAAA 
GTCTCCATCA TACCTCCAAC GGCACTCAAC AGTATCGACT CTGATGGGTT TTGGAAAGCA 
ACATGCCCAC CTTCCTGTGC CAGCCCACTT CTAGTTTTTG TCAATTCCAA AAGCGGAGAC 
AATCAGGGGG TCAAGTTTCT ACGGAGGTTC AAACAGTTAC TTAATCCTGC TCAGGTGTTC 
GACTTAATGA ATGGAGGACC CCATCTAGGT TTACGTTTGT TTCAGAAGTT TGACAATTTC 
CGGATCCTTG TTTGTGGTGG AGATGGAAGT GTTGGTTGGG TTTTATCGGA GATTGATAAG 
CTAAGTTTAC ATAAACAGTG TCAACTGGGC GTACTTCCAC TGGGTACTGG AAATGACTTG 
GCTCGTGTGC TAGGTTGGGG TGCTTCTTGT GATGATGATA CTCAGCTGCC TCAAATTCTG 
GAAAAGTTGG AACGTGCTAG CACAAAAATG CTGGATAGAT GGAGTATTAT GTCATATGAA 
CTTAAATTGC CAACAAAGCC TTCTATATCT CCTTTATCTC CAGAGGAGGA ATCTGAAGAG 
TGTCAATTTC AGATATCTGC ATATGAGGAT TCAGTGGCTA CTCATCTTGC AAAGATTCTA 
AATTCAGACA AGCATTCAGT AGTGATTTCC TCTGCTAAGG TTTTATGTGA AACTGTAAAG 
GACTTTGTTG CAAAAGTCGG GAAGGCTTAT GAGAAACCAA TGGAAAATGC AGAGGAAGCT 
GAATCAATGG CTGTAAAGTG TTCAACTCTG AATGAAAAAT TGGACCTGCT GCTTCAGACT 
CTGCATGCAG AATCTCAGGC TGCTCCATTG CTGGCACATT CCACCCCCCC TATTGTGGAA 
GAAGAAGTTG AGGAAGAGTT TTCCAGTGAA GAATCCCTGT CTGAAAGTAG AGAACAGTTA 
GCTGACAGCA TACCAAAGTC TTCCAGCCAA AGGCTCTTCA AACCAAGAGA GCAACTCATG 
CTGCGGGCTA ACAGCCTGAA AAAAGCTGTC AGGCAGATAA TAGAGCAAGC TGAGAAAGTG 
GTAGATGAGC AGAATGCACA CACTGAGGAA CAAGAAAACC AGTCTCCTGA GGAATACAGA 
AAGGAGTTGG AGGAATCTAA AGAGGAAGAA AAAGATGAAG ACACAAAAGA ATCTGAATTT 
CAGGAGTCTG GCAAGGTTCC CCCACGGTCA CCAGACAGAC AGACAAGCCG CAGTACATAT 
ACTCACACAG GCTCATTCTC AGGGCCACCC TTGGCATCAA GCAAGGAAAA CCTACCTGTG 
TTGAACACTA GAATAATCTG TCCAGGTCTG AGGGCAGGGT TGGCGGCCTC CATCGCTGGG 
AGTTCTATCA TCAATAAAAT GTTACTGGCA AACATAGATC CACTGGGGGC TACTCCATTT 
ATTGACCCTG ATCCAGATTC ACTGGAAGGC TATTCAGAAA AATGTGTAAT GAACAACTAT 
TTTGGAATTG GCCTGGATGC AAAGATATCC TTAGAATTTA ACAACAAGCG AGAAGAACAC 
CCTGAAAAAT GCAGAAGCCG AACTAAAAAT ATGATGTGGT ATGGTGTGCT TGGCACCAAG 
GAACTTCTGC AGCGAACATA CAAAAACTTA GAGCAGAAAG TCCAGCTTGA GTGTGATGGG 
CAGTACATCC CTCTTCCCAG CCTTCAGGGT ATTGCTGTGC TGAACATTCC AAGCTATGCT 
GGCGGCACTA ACTTCTGGGG TGGAACCAAG GAAGATGATA TCTTTGGTGC GCCGTCATTT 
GATGATAAAA TCTTGGAGGT GGTAGCAGTT TTTGGAAGTA TGCAGATGGC AGTATCTAGG 
GTGATAAAGC TGCAACATCA TAGAATAGCA CAGTGCCGGT CAGTGAAGAT CACCATACTT 
GGAGAAGAGG GAGTCCCTGT TCAGGTGGAT GGAGAGGCTT GGATTCAGCC ACCTGGCGTT 
ATTAAAATTG TACATAAAAA CCGAGCTCAG ATGCTGACTA GGGACAGGGC TTTTGAGAAC 
ACCCTGAAGT CCTGGGAAGA TAAGCAGAAG TACGATTCCT GTAAGCTTCC CCTTCGCTCT 
CATCTCTATT CTCAACAAGC TGTTGATTTG GCTTCAGAAG AAGAAGTCAC ACTAATCCAT 
CTTTGCTCGA AAGCTTCAGA GGAGCTTATT ACAAGAATAT GCGAAGCTGC AAAAATCCAT 
AGCTTGCTGG AACAAGAACT GGCTCATGCG GTGAATGCCT GCTCTCATGC ACTAAATAAA 
GCCAATCCCC GGTTTCCCGA GAGCCTAACA AGAAACACTG CCATTGAGGT AGCAGTAACT 
GCAAAAGCTT TGCACAATGA AACAGAATCT CTCTTGGTTG GAAGAGTGCC CCTGCAGTTG 
GAATCCCATC ATGAAGAACT TTTATCCAAT GCCCTTCACA GTGTAGAAGT TGAGCTAACA 
AAACTGGCTG AGATTCCTTG GCTGTACTTT GTCTTACAGC CAGATGAGGA GGATCCTCCT 
CTGGAATACT CTAAAAGAAA CAGCAGAAGC ACAGTATTCC GAATAGTGCC AAAGTTTAAA 
AGGGAGAAGG CCTTAAAGAA TAAGCCCAGC ATTCAGCCTG TTCAAAAATG GGGCACAGAT 
GAAGTGGCAG TTTGGCTGGA TCTTCTCAGT TTGGGAGAGT ACAGAGAAAT CTTCATCCGT 
CATGATATCC GTGGCTCAGA GCTATTGCAC CTGGAGAGGC GGGACCTTAA GGATCTGGGG 
ATATTGAAAG TGGGTCATAT GAAGCGAATT CTACAGGGAA TTAAAGAGCT CAGCAAAAAC 
TCCTCTTTAT CGGACATGTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012813199.1
CDS	882..4622
Translation	MAARRFAVPPAGKRGVGNLADTDCFVFSQQECDKMSGSMREPAVAPGAPAGDESSDSEGEHEGPQKLIRKVSTSGQLRSKTSIKEGLLLKQTSSFQRWKKRYFKLRGRTLYYAKDSKSLIFDEVDLSDASVAESSTKNVNNSFTIITPFRRLILCAENRKEMEDWISSLKSVQSREHYETAQFNVEHFSGMHNWYACSHARPTFCNVCRESLSGVTSHGLSCEVCKFKAHKRCAVRATNNCKWTTLASIGKDIIEDEDGIAMPHQWLEGNLPVSAKCAVCDKTCGSVLRLQDWRCLWCKAMVHTACKDQYPRKCPLGQCKVSIIPPTALNSIDSDGFWKATCPPSCASPLLVFVNSKSGDNQGVKFLRRFKQLLNPAQVFDLMNGGPHLGLRLFQKFDNFRILVCGGDGSVGWVLSEIDKLSLHKQCQLGVLPLGTGNDLARVLGWGASCDDDTQLPQILEKLERASTKMLDRWSIMSYELKLPTKPSISPLSPEEESEECQFQISAYEDSVATHLAKILNSDKHSVVISSAKVLCETVKDFVAKVGKAYEKPMENAEEAESMAVKCSTLNEKLDLLLQTLHAESQAAPLLAHSTPPIVEEEVEEEFSSEESLSESREQLADSIPKSSSQRLFKPREQLMLRANSLKKAVRQIIEQAEKVVDEQNAHTEEQENQSPEEYRKELEESKEEEKDEDTKESEFQESGKVPPRSPDRQTSRSTYTHTGSFSGPPLASSKENLPVLNTRIICPGLRAGLAASIAGSSIINKMLLANIDPLGATPFIDPDPDSLEGYSEKCVMNNYFGIGLDAKISLEFNNKREEHPEKCRSRTKNMMWYGVLGTKELLQRTYKNLEQKVQLECDGQYIPLPSLQGIAVLNIPSYAGGTNFWGGTKEDDIFGAPSFDDKILEVVAVFGSMQMAVSRVIKLQHHRIAQCRSVKITILGEEGVPVQVDGEAWIQPPGVIKIVHKNRAQMLTRDRAFENTLKSWEDKQKYDSCKLPLRSHLYSQQAVDLASEEEVTLIHLCSKASEELITRICEAAKIHSLLEQELAHAVNACSHALNKANPRFPESLTRNTAIEVAVTAKALHNETESLLVGRVPLQLESHHEELLSNALHSVEVELTKLAEIPWLYFVLQPDEEDPPLEYSKRNSRSTVFRIVPKFKREKALKNKPSIQPVQKWGTDEVAVWLDLLSLGEYREIFIRHDIRGSELLHLERRDLKDLGILKVGHMKRILQGIKELSKNSSLSDM

Target ORF information:

RefSeq Version	XM_012957745.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis diacylglycerol kinase, eta (dgkh), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012957745.2

ORF Insert Sequence:

ATGGCGGCCA GGCGCTTCGC TGTGCCCCCG GCCGGTAAAA GGGGTGTAGG GAACCTGGCT 
GACACGGACT GCTTTGTTTT TTCCCAGCAG GAGTGCGACA AGATGTCGGG TTCAATGAGG 
GAGCCTGCGG TGGCCCCTGG AGCTCCTGCG GGAGATGAGT CCTCTGACAG CGAGGGGGAG 
CATGAGGGGC CGCAGAAACT CATTCGCAAA GTGTCCACCT CTGGGCAACT CCGCAGCAAG 
ACAAGTATCA AAGAGGGATT ATTACTGAAG CAAACCAGTT CATTTCAGAG ATGGAAAAAA 
CGCTATTTTA AGCTACGTGG CAGAACCTTG TACTATGCCA AGGATTCAAA GTCTCTAATA 
TTTGATGAAG TTGACCTGTC AGATGCCAGT GTAGCTGAAT CAAGCACAAA AAATGTCAAC 
AACAGCTTCA CGATAATCAC CCCTTTCAGA AGGCTTATAT TGTGTGCTGA GAACAGAAAA 
GAAATGGAGG ATTGGATCAG TTCACTAAAG TCTGTCCAGT CAAGAGAGCA TTATGAGACC 
GCACAGTTTA ATGTGGAACA TTTCTCAGGG ATGCATAACT GGTATGCATG TTCCCATGCC 
CGACCCACCT TCTGTAATGT GTGCAGAGAA AGCCTTTCTG GGGTTACCTC ACATGGCCTT 
TCTTGTGAAG TCTGCAAATT CAAAGCTCAT AAAAGATGTG CAGTTAGAGC AACAAACAAC 
TGCAAGTGGA CCACCCTGGC TTCAATCGGC AAAGATATTA TTGAGGATGA GGATGGTATA 
GCAATGCCTC ACCAGTGGCT AGAGGGTAAT CTGCCTGTTA GTGCCAAATG TGCTGTTTGT 
GACAAAACCT GTGGCAGCGT ATTACGTCTG CAGGACTGGA GGTGCCTTTG GTGTAAAGCA 
ATGGTGCACA CAGCATGCAA GGACCAGTAT CCTCGAAAGT GTCCATTGGG CCAGTGCAAA 
GTCTCCATCA TACCTCCAAC GGCACTCAAC AGTATCGACT CTGATGGGTT TTGGAAAGCA 
ACATGCCCAC CTTCCTGTGC CAGCCCACTT CTAGTTTTTG TCAATTCCAA AAGCGGAGAC 
AATCAGGGGG TCAAGTTTCT ACGGAGGTTC AAACAGTTAC TTAATCCTGC TCAGGTGTTC 
GACTTAATGA ATGGAGGACC CCATCTAGGT TTACGTTTGT TTCAGAAGTT TGACAATTTC 
CGGATCCTTG TTTGTGGTGG AGATGGAAGT GTTGGTTGGG TTTTATCGGA GATTGATAAG 
CTAAGTTTAC ATAAACAGTG TCAACTGGGC GTACTTCCAC TGGGTACTGG AAATGACTTG 
GCTCGTGTGC TAGGTTGGGG TGCTTCTTGT GATGATGATA CTCAGCTGCC TCAAATTCTG 
GAAAAGTTGG AACGTGCTAG CACAAAAATG CTGGATAGAT GGAGTATTAT GTCATATGAA 
CTTAAATTGC CAACAAAGCC TTCTATATCT CCTTTATCTC CAGAGGAGGA ATCTGAAGAG 
TGTCAATTTC AGATATCTGC ATATGAGGAT TCAGTGGCTA CTCATCTTGC AAAGATTCTA 
AATTCAGACA AGCATTCAGT AGTGATTTCC TCTGCTAAGG TTTTATGTGA AACTGTAAAG 
GACTTTGTTG CAAAAGTCGG GAAGGCTTAT GAGAAACCAA TGGAAAATGC AGAGGAAGCT 
GAATCAATGG CTGTAAAGTG TTCAACTCTG AATGAAAAAT TGGACCTGCT GCTTCAGACT 
CTGCATGCAG AATCTCAGGC TGCTCCATTG CTGGCACATT CCACCCCCCC TATTGTGGAA 
GAAGAAGTTG AGGAAGAGTT TTCCAGTGAA GAATCCCTGT CTGAAAGTAG AGAACAGTTA 
GCTGACAGCA TACCAAAGTC TTCCAGCCAA AGGCTCTTCA AACCAAGAGA GCAACTCATG 
CTGCGGGCTA ACAGCCTGAA AAAAGCTGTC AGGCAGATAA TAGAGCAAGC TGAGAAAGTG 
GTAGATGAGC AGAATGCACA CACTGAGGAA CAAGAAAACC AGTCTCCTGA GGAATACAGA 
AAGGAGTTGG AGGAATCTAA AGAGGAAGAA AAAGATGAAG ACACAAAAGA ATCTGAATTT 
CAGGAGTCTG GCAAGGTTCC CCCACGGTCA CCAGACAGAC AGACAAGCCG CAGTACATAT 
ACTCACACAG GCTCATTCTC AGGGCCACCC TTGGCATCAA GCAAGGAAAA CCTACCTGTG 
TTGAACACTA GAATAATCTG TCCAGGTCTG AGGGCAGGGT TGGCGGCCTC CATCGCTGGG 
AGTTCTATCA TCAATAAAAT GTTACTGGCA AACATAGATC CACTGGGGGC TACTCCATTT 
ATTGACCCTG ATCCAGATTC ACTGGAAGGC TATTCAGAAA AATGTGTAAT GAACAACTAT 
TTTGGAATTG GCCTGGATGC AAAGATATCC TTAGAATTTA ACAACAAGCG AGAAGAACAC 
CCTGAAAAAT GCAGAAGCCG AACTAAAAAT ATGATGTGGT ATGGTGTGCT TGGCACCAAG 
GAACTTCTGC AGCGAACATA CAAAAACTTA GAGCAGAAAG TCCAGCTTGA GTGTGATGGG 
CAGTACATCC CTCTTCCCAG CCTTCAGGGT ATTGCTGTGC TGAACATTCC AAGCTATGCT 
GGCGGCACTA ACTTCTGGGG TGGAACCAAG GAAGATGATA TCTTTGGTGC GCCGTCATTT 
GATGATAAAA TCTTGGAGGT GGTAGCAGTT TTTGGAAGTA TGCAGATGGC AGTATCTAGG 
GTGATAAAGC TGCAACATCA TAGAATAGCA CAGTGCCGGT CAGTGAAGAT CACCATACTT 
GGAGAAGAGG GAGTCCCTGT TCAGGTGGAT GGAGAGGCTT GGATTCAGCC ACCTGGCGTT 
ATTAAAATTG TACATAAAAA CCGAGCTCAG ATGCTGACTA GGGACAGGGC TTTTGAGAAC 
ACCCTGAAGT CCTGGGAAGA TAAGCAGAAG TACGATTCCT GTAAGCTTCC CCTTCGCTCT 
CATCTCTATT CTCAACAAGC TGTTGATTTG GCTTCAGAAG AAGAAGTCAC ACTAATCCAT 
CTTTGCTCGA AAGCTTCAGA GGAGCTTATT ACAAGAATAT GCGAAGCTGC AAAAATCCAT 
AGCTTGCTGG AACAAGAACT GGCTCATGCG GTGAATGCCT GCTCTCATGC ACTAAATAAA 
GCCAATCCCC GGTTTCCCGA GAGCCTAACA AGAAACACTG CCATTGAGGT AGCAGTAACT 
GCAAAAGCTT TGCACAATGA AACAGAATCT CTCTTGGTTG GAAGAGTGCC CCTGCAGTTG 
GAATCCCATC ATGAAGAACT TTTATCCAAT GCCCTTCACA GTGTAGAAGT TGAGCTAACA 
AAACTGGCTG AGATTCCTTG GCTGTACTTT GTCTTACAGC CAGATGAGGA GGATCCTCCT 
CTGGAATACT CTAAAAGAAA CAGCAGAAGC ACAGTATTCC GAATAGTGCC AAAGTTTAAA 
AGGGAGAAGG CCTTAAAGAA TAAGCCCAGC ATTCAGCCTG TTCAAAAATG GGGCACAGAT 
GAAGTGGCAG TTTGGCTGGA TCTTCTCAGT TTGGGAGAGT ACAGAGAAAT CTTCATCCGT 
CATGATATCC GTGGCTCAGA GCTATTGCAC CTGGAGAGGC GGGACCTTAA GGATCTGGGG 
ATATTGAAAG TGGGTCATAT GAAGCGAATT CTACAGGGAA TTAAAGAGCT CAGCAAAAAC 
TCCTCTTTAT CGGACATGTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

dgkh ( XM_012957746.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012813200.1 Xenopus tropicalis diacylglycerol kinase, eta (dgkh), transcript variant X3, mRNA.

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CloneID	OXa33657
Clone ID Related Accession (Same CDS sequence)	XM_012957746.2
Accession Version	XM_012957746.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3708bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1473177600000
Organism	Xenopus tropicalis(tropical clawed frog)
Product	diacylglycerol kinase eta isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030678.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012957746.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGGAGA CTGCCTGCCC CTCGTACAGC CGCCGGAGCC CAGTTTGGGA AGAGATGGAG 
TGCGACAAGA TGTCGGGTTC AATGAGGGAG CCTGCGGTGG CCCCTGGAGC TCCTGCGGGA 
GATGAGTCCT CTGACAGCGA GGGGGAGCAT GAGGGGCCGC AGAAACTCAT TCGCAAAGTG 
TCCACCTCTG GGCAACTCCG CAGCAAGACA AGTATCAAAG AGGGATTATT ACTGAAGCAA 
ACCAGTTCAT TTCAGAGATG GAAAAAACGC TATTTTAAGC TACGTGGCAG AACCTTGTAC 
TATGCCAAGG ATTCAAAGTC TCTAATATTT GATGAAGTTG ACCTGTCAGA TGCCAGTGTA 
GCTGAATCAA GCACAAAAAA TGTCAACAAC AGCTTCACGA TAATCACCCC TTTCAGAAGG 
CTTATATTGT GTGCTGAGAA CAGAAAAGAA ATGGAGGATT GGATCAGTTC ACTAAAGTCT 
GTCCAGTCAA GAGAGCATTA TGAGACCGCA CAGTTTAATG TGGAACATTT CTCAGGGATG 
CATAACTGGT ATGCATGTTC CCATGCCCGA CCCACCTTCT GTAATGTGTG CAGAGAAAGC 
CTTTCTGGGG TTACCTCACA TGGCCTTTCT TGTGAAGTCT GCAAATTCAA AGCTCATAAA 
AGATGTGCAG TTAGAGCAAC AAACAACTGC AAGTGGACCA CCCTGGCTTC AATCGGCAAA 
GATATTATTG AGGATGAGGA TGGTATAGCA ATGCCTCACC AGTGGCTAGA GGGTAATCTG 
CCTGTTAGTG CCAAATGTGC TGTTTGTGAC AAAACCTGTG GCAGCGTATT ACGTCTGCAG 
GACTGGAGGT GCCTTTGGTG TAAAGCAATG GTGCACACAG CATGCAAGGA CCAGTATCCT 
CGAAAGTGTC CATTGGGCCA GTGCAAAGTC TCCATCATAC CTCCAACGGC ACTCAACAGT 
ATCGACTCTG ATGGGTTTTG GAAAGCAACA TGCCCACCTT CCTGTGCCAG CCCACTTCTA 
GTTTTTGTCA ATTCCAAAAG CGGAGACAAT CAGGGGGTCA AGTTTCTACG GAGGTTCAAA 
CAGTTACTTA ATCCTGCTCA GGTGTTCGAC TTAATGAATG GAGGACCCCA TCTAGGTTTA 
CGTTTGTTTC AGAAGTTTGA CAATTTCCGG ATCCTTGTTT GTGGTGGAGA TGGAAGTGTT 
GGTTGGGTTT TATCGGAGAT TGATAAGCTA AGTTTACATA AACAGTGTCA ACTGGGCGTA 
CTTCCACTGG GTACTGGAAA TGACTTGGCT CGTGTGCTAG GTTGGGGTGC TTCTTGTGAT 
GATGATACTC AGCTGCCTCA AATTCTGGAA AAGTTGGAAC GTGCTAGCAC AAAAATGCTG 
GATAGATGGA GTATTATGTC ATATGAACTT AAATTGCCAA CAAAGCCTTC TATATCTCCT 
TTATCTCCAG AGGAGGAATC TGAAGAGTGT CAATTTCAGA TATCTGCATA TGAGGATTCA 
GTGGCTACTC ATCTTGCAAA GATTCTAAAT TCAGACAAGC ATTCAGTAGT GATTTCCTCT 
GCTAAGGTTT TATGTGAAAC TGTAAAGGAC TTTGTTGCAA AAGTCGGGAA GGCTTATGAG 
AAACCAATGG AAAATGCAGA GGAAGCTGAA TCAATGGCTG TAAAGTGTTC AACTCTGAAT 
GAAAAATTGG ACCTGCTGCT TCAGACTCTG CATGCAGAAT CTCAGGCTGC TCCATTGCTG 
GCACATTCCA CCCCCCCTAT TGTGGAAGAA GAAGTTGAGG AAGAGTTTTC CAGTGAAGAA 
TCCCTGTCTG AAAGTAGAGA ACAGTTAGCT GACAGCATAC CAAAGTCTTC CAGCCAAAGG 
CTCTTCAAAC CAAGAGAGCA ACTCATGCTG CGGGCTAACA GCCTGAAAAA AGCTGTCAGG 
CAGATAATAG AGCAAGCTGA GAAAGTGGTA GATGAGCAGA ATGCACACAC TGAGGAACAA 
GAAAACCAGT CTCCTGAGGA ATACAGAAAG GAGTTGGAGG AATCTAAAGA GGAAGAAAAA 
GATGAAGACA CAAAAGAATC TGAATTTCAG GAGTCTGGCA AGGTTCCCCC ACGGTCACCA 
GACAGACAGA CAAGCCGCAG TACATATACT CACACAGGCT CATTCTCAGG GCCACCCTTG 
GCATCAAGCA AGGAAAACCT ACCTGTGTTG AACACTAGAA TAATCTGTCC AGGTCTGAGG 
GCAGGGTTGG CGGCCTCCAT CGCTGGGAGT TCTATCATCA ATAAAATGTT ACTGGCAAAC 
ATAGATCCAC TGGGGGCTAC TCCATTTATT GACCCTGATC CAGATTCACT GGAAGGCTAT 
TCAGAAAAAT GTGTAATGAA CAACTATTTT GGAATTGGCC TGGATGCAAA GATATCCTTA 
GAATTTAACA ACAAGCGAGA AGAACACCCT GAAAAATGCA GAAGCCGAAC TAAAAATATG 
ATGTGGTATG GTGTGCTTGG CACCAAGGAA CTTCTGCAGC GAACATACAA AAACTTAGAG 
CAGAAAGTCC AGCTTGAGTG TGATGGGCAG TACATCCCTC TTCCCAGCCT TCAGGGTATT 
GCTGTGCTGA ACATTCCAAG CTATGCTGGC GGCACTAACT TCTGGGGTGG AACCAAGGAA 
GATGATATCT TTGGTGCGCC GTCATTTGAT GATAAAATCT TGGAGGTGGT AGCAGTTTTT 
GGAAGTATGC AGATGGCAGT ATCTAGGGTG ATAAAGCTGC AACATCATAG AATAGCACAG 
TGCCGGTCAG TGAAGATCAC CATACTTGGA GAAGAGGGAG TCCCTGTTCA GGTGGATGGA 
GAGGCTTGGA TTCAGCCACC TGGCGTTATT AAAATTGTAC ATAAAAACCG AGCTCAGATG 
CTGACTAGGG ACAGGGCTTT TGAGAACACC CTGAAGTCCT GGGAAGATAA GCAGAAGTAC 
GATTCCTGTA AGCTTCCCCT TCGCTCTCAT CTCTATTCTC AACAAGCTGT TGATTTGGCT 
TCAGAAGAAG AAGTCACACT AATCCATCTT TGCTCGAAAG CTTCAGAGGA GCTTATTACA 
AGAATATGCG AAGCTGCAAA AATCCATAGC TTGCTGGAAC AAGAACTGGC TCATGCGGTG 
AATGCCTGCT CTCATGCACT AAATAAAGCC AATCCCCGGT TTCCCGAGAG CCTAACAAGA 
AACACTGCCA TTGAGGTAGC AGTAACTGCA AAAGCTTTGC ACAATGAAAC AGAATCTCTC 
TTGGTTGGAA GAGTGCCCCT GCAGTTGGAA TCCCATCATG AAGAACTTTT ATCCAATGCC 
CTTCACAGTG TAGAAGTTGA GCTAACAAAA CTGGCTGAGA TTCCTTGGCT GTACTTTGTC 
TTACAGCCAG ATGAGGAGGA TCCTCCTCTG GAATACTCTA AAAGAAACAG CAGAAGCACA 
GTATTCCGAA TAGTGCCAAA GTTTAAAAGG GAGAAGGCCT TAAAGAATAA GCCCAGCATT 
CAGCCTGTTC AAAAATGGGG CACAGATGAA GTGGCAGTTT GGCTGGATCT TCTCAGTTTG 
GGAGAGTACA GAGAAATCTT CATCCGTCAT GATATCCGTG GCTCAGAGCT ATTGCACCTG 
GAGAGGCGGG ACCTTAAGGA TCTGGGGATA TTGAAAGTGG GTCATATGAA GCGAATTCTA 
CAGGGAATTA AAGAGCTCAG CAAAAACTCC TCTTTATCGG ACATGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012813200.1
CDS	2641..6348
Translation	MMETACPSYSRRSPVWEEMECDKMSGSMREPAVAPGAPAGDESSDSEGEHEGPQKLIRKVSTSGQLRSKTSIKEGLLLKQTSSFQRWKKRYFKLRGRTLYYAKDSKSLIFDEVDLSDASVAESSTKNVNNSFTIITPFRRLILCAENRKEMEDWISSLKSVQSREHYETAQFNVEHFSGMHNWYACSHARPTFCNVCRESLSGVTSHGLSCEVCKFKAHKRCAVRATNNCKWTTLASIGKDIIEDEDGIAMPHQWLEGNLPVSAKCAVCDKTCGSVLRLQDWRCLWCKAMVHTACKDQYPRKCPLGQCKVSIIPPTALNSIDSDGFWKATCPPSCASPLLVFVNSKSGDNQGVKFLRRFKQLLNPAQVFDLMNGGPHLGLRLFQKFDNFRILVCGGDGSVGWVLSEIDKLSLHKQCQLGVLPLGTGNDLARVLGWGASCDDDTQLPQILEKLERASTKMLDRWSIMSYELKLPTKPSISPLSPEEESEECQFQISAYEDSVATHLAKILNSDKHSVVISSAKVLCETVKDFVAKVGKAYEKPMENAEEAESMAVKCSTLNEKLDLLLQTLHAESQAAPLLAHSTPPIVEEEVEEEFSSEESLSESREQLADSIPKSSSQRLFKPREQLMLRANSLKKAVRQIIEQAEKVVDEQNAHTEEQENQSPEEYRKELEESKEEEKDEDTKESEFQESGKVPPRSPDRQTSRSTYTHTGSFSGPPLASSKENLPVLNTRIICPGLRAGLAASIAGSSIINKMLLANIDPLGATPFIDPDPDSLEGYSEKCVMNNYFGIGLDAKISLEFNNKREEHPEKCRSRTKNMMWYGVLGTKELLQRTYKNLEQKVQLECDGQYIPLPSLQGIAVLNIPSYAGGTNFWGGTKEDDIFGAPSFDDKILEVVAVFGSMQMAVSRVIKLQHHRIAQCRSVKITILGEEGVPVQVDGEAWIQPPGVIKIVHKNRAQMLTRDRAFENTLKSWEDKQKYDSCKLPLRSHLYSQQAVDLASEEEVTLIHLCSKASEELITRICEAAKIHSLLEQELAHAVNACSHALNKANPRFPESLTRNTAIEVAVTAKALHNETESLLVGRVPLQLESHHEELLSNALHSVEVELTKLAEIPWLYFVLQPDEEDPPLEYSKRNSRSTVFRIVPKFKREKALKNKPSIQPVQKWGTDEVAVWLDLLSLGEYREIFIRHDIRGSELLHLERRDLKDLGILKVGHMKRILQGIKELSKNSSLSDM

Target ORF information:

RefSeq Version	XM_012957746.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis diacylglycerol kinase, eta (dgkh), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012957746.2

ORF Insert Sequence:

ATGATGGAGA CTGCCTGCCC CTCGTACAGC CGCCGGAGCC CAGTTTGGGA AGAGATGGAG 
TGCGACAAGA TGTCGGGTTC AATGAGGGAG CCTGCGGTGG CCCCTGGAGC TCCTGCGGGA 
GATGAGTCCT CTGACAGCGA GGGGGAGCAT GAGGGGCCGC AGAAACTCAT TCGCAAAGTG 
TCCACCTCTG GGCAACTCCG CAGCAAGACA AGTATCAAAG AGGGATTATT ACTGAAGCAA 
ACCAGTTCAT TTCAGAGATG GAAAAAACGC TATTTTAAGC TACGTGGCAG AACCTTGTAC 
TATGCCAAGG ATTCAAAGTC TCTAATATTT GATGAAGTTG ACCTGTCAGA TGCCAGTGTA 
GCTGAATCAA GCACAAAAAA TGTCAACAAC AGCTTCACGA TAATCACCCC TTTCAGAAGG 
CTTATATTGT GTGCTGAGAA CAGAAAAGAA ATGGAGGATT GGATCAGTTC ACTAAAGTCT 
GTCCAGTCAA GAGAGCATTA TGAGACCGCA CAGTTTAATG TGGAACATTT CTCAGGGATG 
CATAACTGGT ATGCATGTTC CCATGCCCGA CCCACCTTCT GTAATGTGTG CAGAGAAAGC 
CTTTCTGGGG TTACCTCACA TGGCCTTTCT TGTGAAGTCT GCAAATTCAA AGCTCATAAA 
AGATGTGCAG TTAGAGCAAC AAACAACTGC AAGTGGACCA CCCTGGCTTC AATCGGCAAA 
GATATTATTG AGGATGAGGA TGGTATAGCA ATGCCTCACC AGTGGCTAGA GGGTAATCTG 
CCTGTTAGTG CCAAATGTGC TGTTTGTGAC AAAACCTGTG GCAGCGTATT ACGTCTGCAG 
GACTGGAGGT GCCTTTGGTG TAAAGCAATG GTGCACACAG CATGCAAGGA CCAGTATCCT 
CGAAAGTGTC CATTGGGCCA GTGCAAAGTC TCCATCATAC CTCCAACGGC ACTCAACAGT 
ATCGACTCTG ATGGGTTTTG GAAAGCAACA TGCCCACCTT CCTGTGCCAG CCCACTTCTA 
GTTTTTGTCA ATTCCAAAAG CGGAGACAAT CAGGGGGTCA AGTTTCTACG GAGGTTCAAA 
CAGTTACTTA ATCCTGCTCA GGTGTTCGAC TTAATGAATG GAGGACCCCA TCTAGGTTTA 
CGTTTGTTTC AGAAGTTTGA CAATTTCCGG ATCCTTGTTT GTGGTGGAGA TGGAAGTGTT 
GGTTGGGTTT TATCGGAGAT TGATAAGCTA AGTTTACATA AACAGTGTCA ACTGGGCGTA 
CTTCCACTGG GTACTGGAAA TGACTTGGCT CGTGTGCTAG GTTGGGGTGC TTCTTGTGAT 
GATGATACTC AGCTGCCTCA AATTCTGGAA AAGTTGGAAC GTGCTAGCAC AAAAATGCTG 
GATAGATGGA GTATTATGTC ATATGAACTT AAATTGCCAA CAAAGCCTTC TATATCTCCT 
TTATCTCCAG AGGAGGAATC TGAAGAGTGT CAATTTCAGA TATCTGCATA TGAGGATTCA 
GTGGCTACTC ATCTTGCAAA GATTCTAAAT TCAGACAAGC ATTCAGTAGT GATTTCCTCT 
GCTAAGGTTT TATGTGAAAC TGTAAAGGAC TTTGTTGCAA AAGTCGGGAA GGCTTATGAG 
AAACCAATGG AAAATGCAGA GGAAGCTGAA TCAATGGCTG TAAAGTGTTC AACTCTGAAT 
GAAAAATTGG ACCTGCTGCT TCAGACTCTG CATGCAGAAT CTCAGGCTGC TCCATTGCTG 
GCACATTCCA CCCCCCCTAT TGTGGAAGAA GAAGTTGAGG AAGAGTTTTC CAGTGAAGAA 
TCCCTGTCTG AAAGTAGAGA ACAGTTAGCT GACAGCATAC CAAAGTCTTC CAGCCAAAGG 
CTCTTCAAAC CAAGAGAGCA ACTCATGCTG CGGGCTAACA GCCTGAAAAA AGCTGTCAGG 
CAGATAATAG AGCAAGCTGA GAAAGTGGTA GATGAGCAGA ATGCACACAC TGAGGAACAA 
GAAAACCAGT CTCCTGAGGA ATACAGAAAG GAGTTGGAGG AATCTAAAGA GGAAGAAAAA 
GATGAAGACA CAAAAGAATC TGAATTTCAG GAGTCTGGCA AGGTTCCCCC ACGGTCACCA 
GACAGACAGA CAAGCCGCAG TACATATACT CACACAGGCT CATTCTCAGG GCCACCCTTG 
GCATCAAGCA AGGAAAACCT ACCTGTGTTG AACACTAGAA TAATCTGTCC AGGTCTGAGG 
GCAGGGTTGG CGGCCTCCAT CGCTGGGAGT TCTATCATCA ATAAAATGTT ACTGGCAAAC 
ATAGATCCAC TGGGGGCTAC TCCATTTATT GACCCTGATC CAGATTCACT GGAAGGCTAT 
TCAGAAAAAT GTGTAATGAA CAACTATTTT GGAATTGGCC TGGATGCAAA GATATCCTTA 
GAATTTAACA ACAAGCGAGA AGAACACCCT GAAAAATGCA GAAGCCGAAC TAAAAATATG 
ATGTGGTATG GTGTGCTTGG CACCAAGGAA CTTCTGCAGC GAACATACAA AAACTTAGAG 
CAGAAAGTCC AGCTTGAGTG TGATGGGCAG TACATCCCTC TTCCCAGCCT TCAGGGTATT 
GCTGTGCTGA ACATTCCAAG CTATGCTGGC GGCACTAACT TCTGGGGTGG AACCAAGGAA 
GATGATATCT TTGGTGCGCC GTCATTTGAT GATAAAATCT TGGAGGTGGT AGCAGTTTTT 
GGAAGTATGC AGATGGCAGT ATCTAGGGTG ATAAAGCTGC AACATCATAG AATAGCACAG 
TGCCGGTCAG TGAAGATCAC CATACTTGGA GAAGAGGGAG TCCCTGTTCA GGTGGATGGA 
GAGGCTTGGA TTCAGCCACC TGGCGTTATT AAAATTGTAC ATAAAAACCG AGCTCAGATG 
CTGACTAGGG ACAGGGCTTT TGAGAACACC CTGAAGTCCT GGGAAGATAA GCAGAAGTAC 
GATTCCTGTA AGCTTCCCCT TCGCTCTCAT CTCTATTCTC AACAAGCTGT TGATTTGGCT 
TCAGAAGAAG AAGTCACACT AATCCATCTT TGCTCGAAAG CTTCAGAGGA GCTTATTACA 
AGAATATGCG AAGCTGCAAA AATCCATAGC TTGCTGGAAC AAGAACTGGC TCATGCGGTG 
AATGCCTGCT CTCATGCACT AAATAAAGCC AATCCCCGGT TTCCCGAGAG CCTAACAAGA 
AACACTGCCA TTGAGGTAGC AGTAACTGCA AAAGCTTTGC ACAATGAAAC AGAATCTCTC 
TTGGTTGGAA GAGTGCCCCT GCAGTTGGAA TCCCATCATG AAGAACTTTT ATCCAATGCC 
CTTCACAGTG TAGAAGTTGA GCTAACAAAA CTGGCTGAGA TTCCTTGGCT GTACTTTGTC 
TTACAGCCAG ATGAGGAGGA TCCTCCTCTG GAATACTCTA AAAGAAACAG CAGAAGCACA 
GTATTCCGAA TAGTGCCAAA GTTTAAAAGG GAGAAGGCCT TAAAGAATAA GCCCAGCATT 
CAGCCTGTTC AAAAATGGGG CACAGATGAA GTGGCAGTTT GGCTGGATCT TCTCAGTTTG 
GGAGAGTACA GAGAAATCTT CATCCGTCAT GATATCCGTG GCTCAGAGCT ATTGCACCTG 
GAGAGGCGGG ACCTTAAGGA TCTGGGGATA TTGAAAGTGG GTCATATGAA GCGAATTCTA 
CAGGGAATTA AAGAGCTCAG CAAAAACTCC TCTTTATCGG ACATGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

dgkh ( XM_012957748.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012813202.1 Xenopus tropicalis diacylglycerol kinase, eta (dgkh), transcript variant X4, mRNA.

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CloneID	OXa33658
Clone ID Related Accession (Same CDS sequence)	XM_012957748.2
Accession Version	XM_012957748.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3573bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1473177600000
Organism	Xenopus tropicalis(tropical clawed frog)
Product	diacylglycerol kinase eta isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030678.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012957748.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGCCA GGCGCTTCGC TGTGCCCCCG GCCGGTAAAA GGGGTGTAGG GAACCTGGCT 
GACACGGACT GCTTTGTTTT TTCCCAGCAG GAGTGCGACA AGATGTCGGG TTCAATGAGG 
GAGCCTGCGG TGGCCCCTGG AGCTCCTGCG GGAGATGAGT CCTCTGACAG CGAGGGGGAG 
CATGAGGGGC CGCAGAAACT CATTCGCAAA GTGTCCACCT CTGGGCAACT CCGCAGCAAG 
ACAAGTATCA AAGAGGGATT ATTACTGAAG CAAACCAGTT CATTTCAGAG ATGGAAAAAA 
CGCTATTTTA AGCTACGTGG CAGAACCTTG TACTATGCCA AGGATTCAAA GTCTCTAATA 
TTTGATGAAG TTGACCTGTC AGATGCCAGT GTAGCTGAAT CAAGCACAAA AAATGTCAAC 
AACAGCTTCA CGATAATCAC CCCTTTCAGA AGGCTTATAT TGTGTGCTGA GAACAGAAAA 
GAAATGGAGG ATTGGATCAG TTCACTAAAG TCTGTCCAGT CAAGAGAGCA TTATGAGACC 
GCACAGTTTA ATGTGGAACA TTTCTCAGGG ATGCATAACT GGTATGCATG TTCCCATGCC 
CGACCCACCT TCTGTAATGT GTGCAGAGAA AGCCTTTCTG GGGTTACCTC ACATGGCCTT 
TCTTGTGAAG TCTGCAAATT CAAAGCTCAT AAAAGATGTG CAGTTAGAGC AACAAACAAC 
TGCAAGTGGA CCACCCTGGC TTCAATCGGC AAAGATATTA TTGAGGATGA GGATGGTATA 
GCAATGCCTC ACCAGTGGCT AGAGGGTAAT CTGCCTGTTA GTGCCAAATG TGCTGTTTGT 
GACAAAACCT GTGGCAGCGT ATTACGTCTG CAGGACTGGA GGTGCCTTTG GTGTAAAGCA 
ATGGTGCACA CAGCATGCAA GGACCAGTAT CCTCGAAAGT GTCCATTGGG CCAGTGCAAA 
GTCTCCATCA TACCTCCAAC GGCACTCAAC AGTATCGACT CTGATGGGTT TTGGAAAGCA 
ACATGCCCAC CTTCCTGTGC CAGCCCACTT CTAGTTTTTG TCAATTCCAA AAGCGGAGAC 
AATCAGGGGG TCAAGTTTCT ACGGAGGTTC AAACAGTTAC TTAATCCTGC TCAGGTGTTC 
GACTTAATGA ATGGAGGACC CCATCTAGGT TTACGTTTGT TTCAGAAGTT TGACAATTTC 
CGGATCCTTG TTTGTGGTGG AGATGGAAGT GTTGGTTGGG TTTTATCGGA GATTGATAAG 
CTAAGTTTAC ATAAACAGTG TCAACTGGGC GTACTTCCAC TGGGTACTGG AAATGACTTG 
GCTCGTGTGC TAGGTTGGGG TGCTTCTTGT GATGATGATA CTCAGCTGCC TCAAATTCTG 
GAAAAGTTGG AACGTGCTAG CACAAAAATG CTGGATAGAT GGAGTATTAT GTCATATGAA 
CTTAAATTGC CAACAAAGCC TTCTATATCT CCTTTATCTC CAGAGGAGGA ATCTGAAGAG 
TGTCAATTTC AGATATCTGC ATATGAGGAT TCAGTGGCTA CTCATCTTGC AAAGATTCTA 
AATTCAGACA AGCATTCAGT AGTGATTTCC TCTGCTAAGG TTTTATGTGA AACTGTAAAG 
GACTTTGTTG CAAAAGTCGG GAAGGCTTAT GAGAAACCAA TGGAAAATGC AGAGGAAGCT 
GAATCAATGG CTGTAAAGTG TTCAACTCTG AATGAAAAAT TGGACCTGCT GCTTCAGACT 
CTGCATGCAG AATCTCAGGC TGCTCCATTG CTGGCACATT CCACCCCCCC TATTGTGGAA 
GAAGAAGTTG AGGAAGAGTT TTCCAGTGAA GAATCCCTGT CTGAAAGTAG AGAACAGTTA 
GCTGACAGCA TACCAAAGTC TTCCAGCCAA AGGCTCTTCA AACCAAGAGA GCAACTCATG 
CTGCGGGCTA ACAGCCTGAA AAAAGCTGTC AGGCAGATAA TAGAGCAAGC TGAGAAAGTG 
GTAGATGAGC AGAATGCACA CACTGAGGAA CAAGAAAACC AGTCTCCTGA GGAATACAGA 
AAGGAGTTGG AGGAATCTAA AGAGGAAGAA AAAGATGAAG ACACAAAAGA ATCTGAATTT 
CAGGAGTCTG GCAAGGTTCC CCCACGGTCA CCAGACAGAC AGACAAGCCG CAGTACATAT 
ACTCACACAG GCTCATTCTC AGGGCCACCC TTGGCATCAA GCAAGGAAAA CCTACCTGTG 
TTGAACACTA GAATAATCTG TCCAGGTCTG AGGGCAGGGT TGGCGGCCTC CATCGCTGGG 
AGTTCTATCA TCAATAAAAT GTTACTGGCA AACATAGATC CACTGGGGGC TACTCCATTT 
ATTGACCCTG ATCCAGATTC ACTGGAAGGC TATTCAGAAA AATGTGTAAT GAACAACTAT 
TTTGGAATTG GCCTGGATGC AAAGATATCC TTAGAATTTA ACAACAAGCG AGAAGAACAC 
CCTGAAAAAT GCAGAAGCCG AACTAAAAAT ATGATGTGGT ATGGTGTGCT TGGCACCAAG 
GAACTTCTGC AGCGAACATA CAAAAACTTA GAGCAGAAAG TCCAGCTTGA GTGTGATGGG 
CAGTACATCC CTCTTCCCAG CCTTCAGGGT ATTGCTGTGC TGAACATTCC AAGCTATGCT 
GGCGGCACTA ACTTCTGGGG TGGAACCAAG GAAGATGATA TCTTTGGTGC GCCGTCATTT 
GATGATAAAA TCTTGGAGGT GGTAGCAGTT TTTGGAAGTA TGCAGATGGC AGTATCTAGG 
GTGATAAAGC TGCAACATCA TAGAATAGCA CAGTGCCGGT CAGTGAAGAT CACCATACTT 
GGAGAAGAGG GAGTCCCTGT TCAGGTGGAT GGAGAGGCTT GGATTCAGCC ACCTGGCGTT 
ATTAAAATTG TACATAAAAA CCGAGCTCAG ATGCTGACTA GGGACAGGGC TTTTGAGAAC 
ACCCTGAAGT CCTGGGAAGA TAAGCAGAAG TACGATTCCT GTAAGCTTCC CCTTCGCTCT 
CATCTCTATT CTCAACAAGC TGTTGATTTG GCTTCAGAAG AAGAAGTCAC ACTAATCCAT 
CTTTGCTCGA AAGCTTCAGA GGAGCTTATT ACAAGAATAT GCGAAGCTGC AAAAATCCAT 
AGCTTGCTGG AACAAGAACT GGCTCATGCG GTGAATGCCT GCTCTCATGC ACTAAATAAA 
GCCAATCCCC GGTTTCCCGA GAGCCTAACA AGAAACACTG CCATTGAGGT AGCAGTAACT 
GCAAAAGCTT TGCACAATGA AACAGAATCT CTCTTGGTTG GAAGAGTGCC CCTGCAGTTG 
GAATCCCATC ATGAAGAACT TTTATCCAAT GCCCTTCACA GTGTAGAAGT TGAGCTAACA 
AAACTGGCTG AGATTCCTTG GCTGTACTTT GTCTTACAGC CAGATGAGGA GGATCCTCCT 
CTGGAATACT CTAAAAGAAA CAGCAGAAGC ACAGTATTCC GAATAGTGCC AAAGTTTAAA 
AGGGAGAAGG CCTTAAAGAA TAAGCCCAGC ATTCAGCCTG GATCTGGGGA TATTGAAAGT 
GGGTCATATG AAGCGAATTC TACAGGGAAT TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012813202.1
CDS	882..4454
Translation	MAARRFAVPPAGKRGVGNLADTDCFVFSQQECDKMSGSMREPAVAPGAPAGDESSDSEGEHEGPQKLIRKVSTSGQLRSKTSIKEGLLLKQTSSFQRWKKRYFKLRGRTLYYAKDSKSLIFDEVDLSDASVAESSTKNVNNSFTIITPFRRLILCAENRKEMEDWISSLKSVQSREHYETAQFNVEHFSGMHNWYACSHARPTFCNVCRESLSGVTSHGLSCEVCKFKAHKRCAVRATNNCKWTTLASIGKDIIEDEDGIAMPHQWLEGNLPVSAKCAVCDKTCGSVLRLQDWRCLWCKAMVHTACKDQYPRKCPLGQCKVSIIPPTALNSIDSDGFWKATCPPSCASPLLVFVNSKSGDNQGVKFLRRFKQLLNPAQVFDLMNGGPHLGLRLFQKFDNFRILVCGGDGSVGWVLSEIDKLSLHKQCQLGVLPLGTGNDLARVLGWGASCDDDTQLPQILEKLERASTKMLDRWSIMSYELKLPTKPSISPLSPEEESEECQFQISAYEDSVATHLAKILNSDKHSVVISSAKVLCETVKDFVAKVGKAYEKPMENAEEAESMAVKCSTLNEKLDLLLQTLHAESQAAPLLAHSTPPIVEEEVEEEFSSEESLSESREQLADSIPKSSSQRLFKPREQLMLRANSLKKAVRQIIEQAEKVVDEQNAHTEEQENQSPEEYRKELEESKEEEKDEDTKESEFQESGKVPPRSPDRQTSRSTYTHTGSFSGPPLASSKENLPVLNTRIICPGLRAGLAASIAGSSIINKMLLANIDPLGATPFIDPDPDSLEGYSEKCVMNNYFGIGLDAKISLEFNNKREEHPEKCRSRTKNMMWYGVLGTKELLQRTYKNLEQKVQLECDGQYIPLPSLQGIAVLNIPSYAGGTNFWGGTKEDDIFGAPSFDDKILEVVAVFGSMQMAVSRVIKLQHHRIAQCRSVKITILGEEGVPVQVDGEAWIQPPGVIKIVHKNRAQMLTRDRAFENTLKSWEDKQKYDSCKLPLRSHLYSQQAVDLASEEEVTLIHLCSKASEELITRICEAAKIHSLLEQELAHAVNACSHALNKANPRFPESLTRNTAIEVAVTAKALHNETESLLVGRVPLQLESHHEELLSNALHSVEVELTKLAEIPWLYFVLQPDEEDPPLEYSKRNSRSTVFRIVPKFKREKALKNKPSIQPGSGDIESGSYEANSTGN

Target ORF information:

RefSeq Version	XM_012957748.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis diacylglycerol kinase, eta (dgkh), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012957748.2

ORF Insert Sequence:

ATGGCGGCCA GGCGCTTCGC TGTGCCCCCG GCCGGTAAAA GGGGTGTAGG GAACCTGGCT 
GACACGGACT GCTTTGTTTT TTCCCAGCAG GAGTGCGACA AGATGTCGGG TTCAATGAGG 
GAGCCTGCGG TGGCCCCTGG AGCTCCTGCG GGAGATGAGT CCTCTGACAG CGAGGGGGAG 
CATGAGGGGC CGCAGAAACT CATTCGCAAA GTGTCCACCT CTGGGCAACT CCGCAGCAAG 
ACAAGTATCA AAGAGGGATT ATTACTGAAG CAAACCAGTT CATTTCAGAG ATGGAAAAAA 
CGCTATTTTA AGCTACGTGG CAGAACCTTG TACTATGCCA AGGATTCAAA GTCTCTAATA 
TTTGATGAAG TTGACCTGTC AGATGCCAGT GTAGCTGAAT CAAGCACAAA AAATGTCAAC 
AACAGCTTCA CGATAATCAC CCCTTTCAGA AGGCTTATAT TGTGTGCTGA GAACAGAAAA 
GAAATGGAGG ATTGGATCAG TTCACTAAAG TCTGTCCAGT CAAGAGAGCA TTATGAGACC 
GCACAGTTTA ATGTGGAACA TTTCTCAGGG ATGCATAACT GGTATGCATG TTCCCATGCC 
CGACCCACCT TCTGTAATGT GTGCAGAGAA AGCCTTTCTG GGGTTACCTC ACATGGCCTT 
TCTTGTGAAG TCTGCAAATT CAAAGCTCAT AAAAGATGTG CAGTTAGAGC AACAAACAAC 
TGCAAGTGGA CCACCCTGGC TTCAATCGGC AAAGATATTA TTGAGGATGA GGATGGTATA 
GCAATGCCTC ACCAGTGGCT AGAGGGTAAT CTGCCTGTTA GTGCCAAATG TGCTGTTTGT 
GACAAAACCT GTGGCAGCGT ATTACGTCTG CAGGACTGGA GGTGCCTTTG GTGTAAAGCA 
ATGGTGCACA CAGCATGCAA GGACCAGTAT CCTCGAAAGT GTCCATTGGG CCAGTGCAAA 
GTCTCCATCA TACCTCCAAC GGCACTCAAC AGTATCGACT CTGATGGGTT TTGGAAAGCA 
ACATGCCCAC CTTCCTGTGC CAGCCCACTT CTAGTTTTTG TCAATTCCAA AAGCGGAGAC 
AATCAGGGGG TCAAGTTTCT ACGGAGGTTC AAACAGTTAC TTAATCCTGC TCAGGTGTTC 
GACTTAATGA ATGGAGGACC CCATCTAGGT TTACGTTTGT TTCAGAAGTT TGACAATTTC 
CGGATCCTTG TTTGTGGTGG AGATGGAAGT GTTGGTTGGG TTTTATCGGA GATTGATAAG 
CTAAGTTTAC ATAAACAGTG TCAACTGGGC GTACTTCCAC TGGGTACTGG AAATGACTTG 
GCTCGTGTGC TAGGTTGGGG TGCTTCTTGT GATGATGATA CTCAGCTGCC TCAAATTCTG 
GAAAAGTTGG AACGTGCTAG CACAAAAATG CTGGATAGAT GGAGTATTAT GTCATATGAA 
CTTAAATTGC CAACAAAGCC TTCTATATCT CCTTTATCTC CAGAGGAGGA ATCTGAAGAG 
TGTCAATTTC AGATATCTGC ATATGAGGAT TCAGTGGCTA CTCATCTTGC AAAGATTCTA 
AATTCAGACA AGCATTCAGT AGTGATTTCC TCTGCTAAGG TTTTATGTGA AACTGTAAAG 
GACTTTGTTG CAAAAGTCGG GAAGGCTTAT GAGAAACCAA TGGAAAATGC AGAGGAAGCT 
GAATCAATGG CTGTAAAGTG TTCAACTCTG AATGAAAAAT TGGACCTGCT GCTTCAGACT 
CTGCATGCAG AATCTCAGGC TGCTCCATTG CTGGCACATT CCACCCCCCC TATTGTGGAA 
GAAGAAGTTG AGGAAGAGTT TTCCAGTGAA GAATCCCTGT CTGAAAGTAG AGAACAGTTA 
GCTGACAGCA TACCAAAGTC TTCCAGCCAA AGGCTCTTCA AACCAAGAGA GCAACTCATG 
CTGCGGGCTA ACAGCCTGAA AAAAGCTGTC AGGCAGATAA TAGAGCAAGC TGAGAAAGTG 
GTAGATGAGC AGAATGCACA CACTGAGGAA CAAGAAAACC AGTCTCCTGA GGAATACAGA 
AAGGAGTTGG AGGAATCTAA AGAGGAAGAA AAAGATGAAG ACACAAAAGA ATCTGAATTT 
CAGGAGTCTG GCAAGGTTCC CCCACGGTCA CCAGACAGAC AGACAAGCCG CAGTACATAT 
ACTCACACAG GCTCATTCTC AGGGCCACCC TTGGCATCAA GCAAGGAAAA CCTACCTGTG 
TTGAACACTA GAATAATCTG TCCAGGTCTG AGGGCAGGGT TGGCGGCCTC CATCGCTGGG 
AGTTCTATCA TCAATAAAAT GTTACTGGCA AACATAGATC CACTGGGGGC TACTCCATTT 
ATTGACCCTG ATCCAGATTC ACTGGAAGGC TATTCAGAAA AATGTGTAAT GAACAACTAT 
TTTGGAATTG GCCTGGATGC AAAGATATCC TTAGAATTTA ACAACAAGCG AGAAGAACAC 
CCTGAAAAAT GCAGAAGCCG AACTAAAAAT ATGATGTGGT ATGGTGTGCT TGGCACCAAG 
GAACTTCTGC AGCGAACATA CAAAAACTTA GAGCAGAAAG TCCAGCTTGA GTGTGATGGG 
CAGTACATCC CTCTTCCCAG CCTTCAGGGT ATTGCTGTGC TGAACATTCC AAGCTATGCT 
GGCGGCACTA ACTTCTGGGG TGGAACCAAG GAAGATGATA TCTTTGGTGC GCCGTCATTT 
GATGATAAAA TCTTGGAGGT GGTAGCAGTT TTTGGAAGTA TGCAGATGGC AGTATCTAGG 
GTGATAAAGC TGCAACATCA TAGAATAGCA CAGTGCCGGT CAGTGAAGAT CACCATACTT 
GGAGAAGAGG GAGTCCCTGT TCAGGTGGAT GGAGAGGCTT GGATTCAGCC ACCTGGCGTT 
ATTAAAATTG TACATAAAAA CCGAGCTCAG ATGCTGACTA GGGACAGGGC TTTTGAGAAC 
ACCCTGAAGT CCTGGGAAGA TAAGCAGAAG TACGATTCCT GTAAGCTTCC CCTTCGCTCT 
CATCTCTATT CTCAACAAGC TGTTGATTTG GCTTCAGAAG AAGAAGTCAC ACTAATCCAT 
CTTTGCTCGA AAGCTTCAGA GGAGCTTATT ACAAGAATAT GCGAAGCTGC AAAAATCCAT 
AGCTTGCTGG AACAAGAACT GGCTCATGCG GTGAATGCCT GCTCTCATGC ACTAAATAAA 
GCCAATCCCC GGTTTCCCGA GAGCCTAACA AGAAACACTG CCATTGAGGT AGCAGTAACT 
GCAAAAGCTT TGCACAATGA AACAGAATCT CTCTTGGTTG GAAGAGTGCC CCTGCAGTTG 
GAATCCCATC ATGAAGAACT TTTATCCAAT GCCCTTCACA GTGTAGAAGT TGAGCTAACA 
AAACTGGCTG AGATTCCTTG GCTGTACTTT GTCTTACAGC CAGATGAGGA GGATCCTCCT 
CTGGAATACT CTAAAAGAAA CAGCAGAAGC ACAGTATTCC GAATAGTGCC AAAGTTTAAA 
AGGGAGAAGG CCTTAAAGAA TAAGCCCAGC ATTCAGCCTG GATCTGGGGA TATTGAAAGT 
GGGTCATATG AAGCGAATTC TACAGGGAAT TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

dgkh ( XM_012957745.3 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012813199.1 Xenopus tropicalis diacylglycerol kinase, eta (dgkh), transcript variant X1, mRNA.

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CloneID	OXa52617
Clone ID Related Accession (Same CDS sequence)	XM_012957745.3
Accession Version	XM_012957745.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3741bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1576512000000
Organism	Xenopus tropicalis(tropical clawed frog)
Product	diacylglycerol kinase eta isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030678.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012957745.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGCCA GGCGCTTCGC TGTGCCCCCG GCCGGTAAAA GGGGTGTAGG GAACCTGGCT 
GACACGGACT GCTTTGTTTT TTCCCAGCAG GAGTGCGACA AGATGTCGGG TTCAATGAGG 
GAGCCTGCGG TGGCCCCTGG AGCTCCTGCG GGAGATGAGT CCTCTGACAG CGAGGGGGAG 
CATGAGGGGC CGCAGAAACT CATTCGCAAA GTGTCCACCT CTGGGCAACT CCGCAGCAAG 
ACAAGTATCA AAGAGGGATT ATTACTGAAG CAAACCAGTT CATTTCAGAG ATGGAAAAAA 
CGCTATTTTA AGCTACGTGG CAGAACCTTG TACTATGCCA AGGATTCAAA GTCTCTAATA 
TTTGATGAAG TTGACCTGTC AGATGCCAGT GTAGCTGAAT CAAGCACAAA AAATGTCAAC 
AACAGCTTCA CGATAATCAC CCCTTTCAGA AGGCTTATAT TGTGTGCTGA GAACAGAAAA 
GAAATGGAGG ATTGGATCAG TTCACTAAAG TCTGTCCAGT CAAGAGAGCA TTATGAGACC 
GCACAGTTTA ATGTGGAACA TTTCTCAGGG ATGCATAACT GGTATGCATG TTCCCATGCC 
CGACCCACCT TCTGTAATGT GTGCAGAGAA AGCCTTTCTG GGGTTACCTC ACATGGCCTT 
TCTTGTGAAG TCTGCAAATT CAAAGCTCAT AAAAGATGTG CAGTTAGAGC AACAAACAAC 
TGCAAGTGGA CCACCCTGGC TTCAATCGGC AAAGATATTA TTGAGGATGA GGATGGTATA 
GCAATGCCTC ACCAGTGGCT AGAGGGTAAT CTGCCTGTTA GTGCCAAATG TGCTGTTTGT 
GACAAAACCT GTGGCAGCGT ATTACGTCTG CAGGACTGGA GGTGCCTTTG GTGTAAAGCA 
ATGGTGCACA CAGCATGCAA GGACCAGTAT CCTCGAAAGT GTCCATTGGG CCAGTGCAAA 
GTCTCCATCA TACCTCCAAC GGCACTCAAC AGTATCGACT CTGATGGGTT TTGGAAAGCA 
ACATGCCCAC CTTCCTGTGC CAGCCCACTT CTAGTTTTTG TCAATTCCAA AAGCGGAGAC 
AATCAGGGGG TCAAGTTTCT ACGGAGGTTC AAACAGTTAC TTAATCCTGC TCAGGTGTTC 
GACTTAATGA ATGGAGGACC CCATCTAGGT TTACGTTTGT TTCAGAAGTT TGACAATTTC 
CGGATCCTTG TTTGTGGTGG AGATGGAAGT GTTGGTTGGG TTTTATCGGA GATTGATAAG 
CTAAGTTTAC ATAAACAGTG TCAACTGGGC GTACTTCCAC TGGGTACTGG AAATGACTTG 
GCTCGTGTGC TAGGTTGGGG TGCTTCTTGT GATGATGATA CTCAGCTGCC TCAAATTCTG 
GAAAAGTTGG AACGTGCTAG CACAAAAATG CTGGATAGAT GGAGTATTAT GTCATATGAA 
CTTAAATTGC CAACAAAGCC TTCTATATCT CCTTTATCTC CAGAGGAGGA ATCTGAAGAG 
TGTCAATTTC AGATATCTGC ATATGAGGAT TCAGTGGCTA CTCATCTTGC AAAGATTCTA 
AATTCAGACA AGCATTCAGT AGTGATTTCC TCTGCTAAGG TTTTATGTGA AACTGTAAAG 
GACTTTGTTG CAAAAGTCGG GAAGGCTTAT GAGAAACCAA TGGAAAATGC AGAGGAAGCT 
GAATCAATGG CTGTAAAGTG TTCAACTCTG AATGAAAAAT TGGACCTGCT GCTTCAGACT 
CTGCATGCAG AATCTCAGGC TGCTCCATTG CTGGCACATT CCACCCCCCC TATTGTGGAA 
GAAGAAGTTG AGGAAGAGTT TTCCAGTGAA GAATCCCTGT CTGAAAGTAG AGAACAGTTA 
GCTGACAGCA TACCAAAGTC TTCCAGCCAA AGGCTCTTCA AACCAAGAGA GCAACTCATG 
CTGCGGGCTA ACAGCCTGAA AAAAGCTGTC AGGCAGATAA TAGAGCAAGC TGAGAAAGTG 
GTAGATGAGC AGAATGCACA CACTGAGGAA CAAGAAAACC AGTCTCCTGA GGAATACAGA 
AAGGAGTTGG AGGAATCTAA AGAGGAAGAA AAAGATGAAG ACACAAAAGA ATCTGAATTT 
CAGGAGTCTG GCAAGGTTCC CCCACGGTCA CCAGACAGAC AGACAAGCCG CAGTACATAT 
ACTCACACAG GCTCATTCTC AGGGCCACCC TTGGCATCAA GCAAGGAAAA CCTACCTGTG 
TTGAACACTA GAATAATCTG TCCAGGTCTG AGGGCAGGGT TGGCGGCCTC CATCGCTGGG 
AGTTCTATCA TCAATAAAAT GTTACTGGCA AACATAGATC CACTGGGGGC TACTCCATTT 
ATTGACCCTG ATCCAGATTC ACTGGAAGGC TATTCAGAAA AATGTGTAAT GAACAACTAT 
TTTGGAATTG GCCTGGATGC AAAGATATCC TTAGAATTTA ACAACAAGCG AGAAGAACAC 
CCTGAAAAAT GCAGAAGCCG AACTAAAAAT ATGATGTGGT ATGGTGTGCT TGGCACCAAG 
GAACTTCTGC AGCGAACATA CAAAAACTTA GAGCAGAAAG TCCAGCTTGA GTGTGATGGG 
CAGTACATCC CTCTTCCCAG CCTTCAGGGT ATTGCTGTGC TGAACATTCC AAGCTATGCT 
GGCGGCACTA ACTTCTGGGG TGGAACCAAG GAAGATGATA TCTTTGGTGC GCCGTCATTT 
GATGATAAAA TCTTGGAGGT GGTAGCAGTT TTTGGAAGTA TGCAGATGGC AGTATCTAGG 
GTGATAAAGC TGCAACATCA TAGAATAGCA CAGTGCCGGT CAGTGAAGAT CACCATACTT 
GGAGAAGAGG GAGTCCCTGT TCAGGTGGAT GGAGAGGCTT GGATTCAGCC ACCTGGCGTT 
ATTAAAATTG TACATAAAAA CCGAGCTCAG ATGCTGACTA GGGACAGGGC TTTTGAGAAC 
ACCCTGAAGT CCTGGGAAGA TAAGCAGAAG TACGATTCCT GTAAGCTTCC CCTTCGCTCT 
CATCTCTATT CTCAACAAGC TGTTGATTTG GCTTCAGAAG AAGAAGTCAC ACTAATCCAT 
CTTTGCTCGA AAGCTTCAGA GGAGCTTATT ACAAGAATAT GCGAAGCTGC AAAAATCCAT 
AGCTTGCTGG AACAAGAACT GGCTCATGCG GTGAATGCCT GCTCTCATGC ACTAAATAAA 
GCCAATCCCC GGTTTCCCGA GAGCCTAACA AGAAACACTG CCATTGAGGT AGCAGTAACT 
GCAAAAGCTT TGCACAATGA AACAGAATCT CTCTTGGTTG GAAGAGTGCC CCTGCAGTTG 
GAATCCCATC ATGAAGAACT TCTATCCAAT GCCCTTCACA GTGTAGAAGT TGAGCTAACA 
AAACTGGCTG AGATTCCTTG GCTGTACTTT GTCTTACAGC CAGATGAGGA GGATCCTCCT 
CTGGAATACT CTAAAAGAAA CAGCAGAAGC ACAGTATTCC GAATAGTGCC AAAGTTTAAA 
AGGGAGAAGG CCTTAAAGAA TAAGCCCAGC ATTCAGCCTG TTCAAAAATG GGGCACAGAT 
GAAGTGGCAG TTTGGCTGGA TCTTCTCAGT TTGGGAGAGT ACAGAGAAAT CTTCATCCGT 
CATGATATCC GTGGCTCAGA GCTATTGCAC CTGGAGAGGC GGGACCTTAA GGATCTGGGG 
ATATTGAAAG TGGGTCATAT GAAGCGAATT CTACAGGGAA TTAAAGAGCT CAGCAAAAAC 
TCCTCTTTAT CGGACATGTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012813199.1
CDS	23..3763
Translation	MAARRFAVPPAGKRGVGNLADTDCFVFSQQECDKMSGSMREPAVAPGAPAGDESSDSEGEHEGPQKLIRKVSTSGQLRSKTSIKEGLLLKQTSSFQRWKKRYFKLRGRTLYYAKDSKSLIFDEVDLSDASVAESSTKNVNNSFTIITPFRRLILCAENRKEMEDWISSLKSVQSREHYETAQFNVEHFSGMHNWYACSHARPTFCNVCRESLSGVTSHGLSCEVCKFKAHKRCAVRATNNCKWTTLASIGKDIIEDEDGIAMPHQWLEGNLPVSAKCAVCDKTCGSVLRLQDWRCLWCKAMVHTACKDQYPRKCPLGQCKVSIIPPTALNSIDSDGFWKATCPPSCASPLLVFVNSKSGDNQGVKFLRRFKQLLNPAQVFDLMNGGPHLGLRLFQKFDNFRILVCGGDGSVGWVLSEIDKLSLHKQCQLGVLPLGTGNDLARVLGWGASCDDDTQLPQILEKLERASTKMLDRWSIMSYELKLPTKPSISPLSPEEESEECQFQISAYEDSVATHLAKILNSDKHSVVISSAKVLCETVKDFVAKVGKAYEKPMENAEEAESMAVKCSTLNEKLDLLLQTLHAESQAAPLLAHSTPPIVEEEVEEEFSSEESLSESREQLADSIPKSSSQRLFKPREQLMLRANSLKKAVRQIIEQAEKVVDEQNAHTEEQENQSPEEYRKELEESKEEEKDEDTKESEFQESGKVPPRSPDRQTSRSTYTHTGSFSGPPLASSKENLPVLNTRIICPGLRAGLAASIAGSSIINKMLLANIDPLGATPFIDPDPDSLEGYSEKCVMNNYFGIGLDAKISLEFNNKREEHPEKCRSRTKNMMWYGVLGTKELLQRTYKNLEQKVQLECDGQYIPLPSLQGIAVLNIPSYAGGTNFWGGTKEDDIFGAPSFDDKILEVVAVFGSMQMAVSRVIKLQHHRIAQCRSVKITILGEEGVPVQVDGEAWIQPPGVIKIVHKNRAQMLTRDRAFENTLKSWEDKQKYDSCKLPLRSHLYSQQAVDLASEEEVTLIHLCSKASEELITRICEAAKIHSLLEQELAHAVNACSHALNKANPRFPESLTRNTAIEVAVTAKALHNETESLLVGRVPLQLESHHEELLSNALHSVEVELTKLAEIPWLYFVLQPDEEDPPLEYSKRNSRSTVFRIVPKFKREKALKNKPSIQPVQKWGTDEVAVWLDLLSLGEYREIFIRHDIRGSELLHLERRDLKDLGILKVGHMKRILQGIKELSKNSSLSDM

Target ORF information:

RefSeq Version	XM_012957745.3
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis diacylglycerol kinase, eta (dgkh), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012957745.3

ORF Insert Sequence:

ATGGCGGCCA GGCGCTTCGC TGTGCCCCCG GCCGGTAAAA GGGGTGTAGG GAACCTGGCT 
GACACGGACT GCTTTGTTTT TTCCCAGCAG GAGTGCGACA AGATGTCGGG TTCAATGAGG 
GAGCCTGCGG TGGCCCCTGG AGCTCCTGCG GGAGATGAGT CCTCTGACAG CGAGGGGGAG 
CATGAGGGGC CGCAGAAACT CATTCGCAAA GTGTCCACCT CTGGGCAACT CCGCAGCAAG 
ACAAGTATCA AAGAGGGATT ATTACTGAAG CAAACCAGTT CATTTCAGAG ATGGAAAAAA 
CGCTATTTTA AGCTACGTGG CAGAACCTTG TACTATGCCA AGGATTCAAA GTCTCTAATA 
TTTGATGAAG TTGACCTGTC AGATGCCAGT GTAGCTGAAT CAAGCACAAA AAATGTCAAC 
AACAGCTTCA CGATAATCAC CCCTTTCAGA AGGCTTATAT TGTGTGCTGA GAACAGAAAA 
GAAATGGAGG ATTGGATCAG TTCACTAAAG TCTGTCCAGT CAAGAGAGCA TTATGAGACC 
GCACAGTTTA ATGTGGAACA TTTCTCAGGG ATGCATAACT GGTATGCATG TTCCCATGCC 
CGACCCACCT TCTGTAATGT GTGCAGAGAA AGCCTTTCTG GGGTTACCTC ACATGGCCTT 
TCTTGTGAAG TCTGCAAATT CAAAGCTCAT AAAAGATGTG CAGTTAGAGC AACAAACAAC 
TGCAAGTGGA CCACCCTGGC TTCAATCGGC AAAGATATTA TTGAGGATGA GGATGGTATA 
GCAATGCCTC ACCAGTGGCT AGAGGGTAAT CTGCCTGTTA GTGCCAAATG TGCTGTTTGT 
GACAAAACCT GTGGCAGCGT ATTACGTCTG CAGGACTGGA GGTGCCTTTG GTGTAAAGCA 
ATGGTGCACA CAGCATGCAA GGACCAGTAT CCTCGAAAGT GTCCATTGGG CCAGTGCAAA 
GTCTCCATCA TACCTCCAAC GGCACTCAAC AGTATCGACT CTGATGGGTT TTGGAAAGCA 
ACATGCCCAC CTTCCTGTGC CAGCCCACTT CTAGTTTTTG TCAATTCCAA AAGCGGAGAC 
AATCAGGGGG TCAAGTTTCT ACGGAGGTTC AAACAGTTAC TTAATCCTGC TCAGGTGTTC 
GACTTAATGA ATGGAGGACC CCATCTAGGT TTACGTTTGT TTCAGAAGTT TGACAATTTC 
CGGATCCTTG TTTGTGGTGG AGATGGAAGT GTTGGTTGGG TTTTATCGGA GATTGATAAG 
CTAAGTTTAC ATAAACAGTG TCAACTGGGC GTACTTCCAC TGGGTACTGG AAATGACTTG 
GCTCGTGTGC TAGGTTGGGG TGCTTCTTGT GATGATGATA CTCAGCTGCC TCAAATTCTG 
GAAAAGTTGG AACGTGCTAG CACAAAAATG CTGGATAGAT GGAGTATTAT GTCATATGAA 
CTTAAATTGC CAACAAAGCC TTCTATATCT CCTTTATCTC CAGAGGAGGA ATCTGAAGAG 
TGTCAATTTC AGATATCTGC ATATGAGGAT TCAGTGGCTA CTCATCTTGC AAAGATTCTA 
AATTCAGACA AGCATTCAGT AGTGATTTCC TCTGCTAAGG TTTTATGTGA AACTGTAAAG 
GACTTTGTTG CAAAAGTCGG GAAGGCTTAT GAGAAACCAA TGGAAAATGC AGAGGAAGCT 
GAATCAATGG CTGTAAAGTG TTCAACTCTG AATGAAAAAT TGGACCTGCT GCTTCAGACT 
CTGCATGCAG AATCTCAGGC TGCTCCATTG CTGGCACATT CCACCCCCCC TATTGTGGAA 
GAAGAAGTTG AGGAAGAGTT TTCCAGTGAA GAATCCCTGT CTGAAAGTAG AGAACAGTTA 
GCTGACAGCA TACCAAAGTC TTCCAGCCAA AGGCTCTTCA AACCAAGAGA GCAACTCATG 
CTGCGGGCTA ACAGCCTGAA AAAAGCTGTC AGGCAGATAA TAGAGCAAGC TGAGAAAGTG 
GTAGATGAGC AGAATGCACA CACTGAGGAA CAAGAAAACC AGTCTCCTGA GGAATACAGA 
AAGGAGTTGG AGGAATCTAA AGAGGAAGAA AAAGATGAAG ACACAAAAGA ATCTGAATTT 
CAGGAGTCTG GCAAGGTTCC CCCACGGTCA CCAGACAGAC AGACAAGCCG CAGTACATAT 
ACTCACACAG GCTCATTCTC AGGGCCACCC TTGGCATCAA GCAAGGAAAA CCTACCTGTG 
TTGAACACTA GAATAATCTG TCCAGGTCTG AGGGCAGGGT TGGCGGCCTC CATCGCTGGG 
AGTTCTATCA TCAATAAAAT GTTACTGGCA AACATAGATC CACTGGGGGC TACTCCATTT 
ATTGACCCTG ATCCAGATTC ACTGGAAGGC TATTCAGAAA AATGTGTAAT GAACAACTAT 
TTTGGAATTG GCCTGGATGC AAAGATATCC TTAGAATTTA ACAACAAGCG AGAAGAACAC 
CCTGAAAAAT GCAGAAGCCG AACTAAAAAT ATGATGTGGT ATGGTGTGCT TGGCACCAAG 
GAACTTCTGC AGCGAACATA CAAAAACTTA GAGCAGAAAG TCCAGCTTGA GTGTGATGGG 
CAGTACATCC CTCTTCCCAG CCTTCAGGGT ATTGCTGTGC TGAACATTCC AAGCTATGCT 
GGCGGCACTA ACTTCTGGGG TGGAACCAAG GAAGATGATA TCTTTGGTGC GCCGTCATTT 
GATGATAAAA TCTTGGAGGT GGTAGCAGTT TTTGGAAGTA TGCAGATGGC AGTATCTAGG 
GTGATAAAGC TGCAACATCA TAGAATAGCA CAGTGCCGGT CAGTGAAGAT CACCATACTT 
GGAGAAGAGG GAGTCCCTGT TCAGGTGGAT GGAGAGGCTT GGATTCAGCC ACCTGGCGTT 
ATTAAAATTG TACATAAAAA CCGAGCTCAG ATGCTGACTA GGGACAGGGC TTTTGAGAAC 
ACCCTGAAGT CCTGGGAAGA TAAGCAGAAG TACGATTCCT GTAAGCTTCC CCTTCGCTCT 
CATCTCTATT CTCAACAAGC TGTTGATTTG GCTTCAGAAG AAGAAGTCAC ACTAATCCAT 
CTTTGCTCGA AAGCTTCAGA GGAGCTTATT ACAAGAATAT GCGAAGCTGC AAAAATCCAT 
AGCTTGCTGG AACAAGAACT GGCTCATGCG GTGAATGCCT GCTCTCATGC ACTAAATAAA 
GCCAATCCCC GGTTTCCCGA GAGCCTAACA AGAAACACTG CCATTGAGGT AGCAGTAACT 
GCAAAAGCTT TGCACAATGA AACAGAATCT CTCTTGGTTG GAAGAGTGCC CCTGCAGTTG 
GAATCCCATC ATGAAGAACT TCTATCCAAT GCCCTTCACA GTGTAGAAGT TGAGCTAACA 
AAACTGGCTG AGATTCCTTG GCTGTACTTT GTCTTACAGC CAGATGAGGA GGATCCTCCT 
CTGGAATACT CTAAAAGAAA CAGCAGAAGC ACAGTATTCC GAATAGTGCC AAAGTTTAAA 
AGGGAGAAGG CCTTAAAGAA TAAGCCCAGC ATTCAGCCTG TTCAAAAATG GGGCACAGAT 
GAAGTGGCAG TTTGGCTGGA TCTTCTCAGT TTGGGAGAGT ACAGAGAAAT CTTCATCCGT 
CATGATATCC GTGGCTCAGA GCTATTGCAC CTGGAGAGGC GGGACCTTAA GGATCTGGGG 
ATATTGAAAG TGGGTCATAT GAAGCGAATT CTACAGGGAA TTAAAGAGCT CAGCAAAAAC 
TCCTCTTTAT CGGACATGTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

dgkh ( XM_004911834.4 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_004911891.1 Xenopus tropicalis diacylglycerol kinase, eta (dgkh), transcript variant X2, mRNA.

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CloneID	OXa52618
Clone ID Related Accession (Same CDS sequence)	XM_004911834.4
Accession Version	XM_004911834.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3711bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1576512000000
Organism	Xenopus tropicalis(tropical clawed frog)
Product	diacylglycerol kinase eta isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030678.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_004911834.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGGAGA CTGCCTGCCC CTCGTACAGC CGCCGGAGCC CAGTTTGGGA AGAGATGCAG 
GAGTGCGACA AGATGTCGGG TTCAATGAGG GAGCCTGCGG TGGCCCCTGG AGCTCCTGCG 
GGAGATGAGT CCTCTGACAG CGAGGGGGAG CATGAGGGGC CGCAGAAACT CATTCGCAAA 
GTGTCCACCT CTGGGCAACT CCGCAGCAAG ACAAGTATCA AAGAGGGATT ATTACTGAAG 
CAAACCAGTT CATTTCAGAG ATGGAAAAAA CGCTATTTTA AGCTACGTGG CAGAACCTTG 
TACTATGCCA AGGATTCAAA GTCTCTAATA TTTGATGAAG TTGACCTGTC AGATGCCAGT 
GTAGCTGAAT CAAGCACAAA AAATGTCAAC AACAGCTTCA CGATAATCAC CCCTTTCAGA 
AGGCTTATAT TGTGTGCTGA GAACAGAAAA GAAATGGAGG ATTGGATCAG TTCACTAAAG 
TCTGTCCAGT CAAGAGAGCA TTATGAGACC GCACAGTTTA ATGTGGAACA TTTCTCAGGG 
ATGCATAACT GGTATGCATG TTCCCATGCC CGACCCACCT TCTGTAATGT GTGCAGAGAA 
AGCCTTTCTG GGGTTACCTC ACATGGCCTT TCTTGTGAAG TCTGCAAATT CAAAGCTCAT 
AAAAGATGTG CAGTTAGAGC AACAAACAAC TGCAAGTGGA CCACCCTGGC TTCAATCGGC 
AAAGATATTA TTGAGGATGA GGATGGTATA GCAATGCCTC ACCAGTGGCT AGAGGGTAAT 
CTGCCTGTTA GTGCCAAATG TGCTGTTTGT GACAAAACCT GTGGCAGCGT ATTACGTCTG 
CAGGACTGGA GGTGCCTTTG GTGTAAAGCA ATGGTGCACA CAGCATGCAA GGACCAGTAT 
CCTCGAAAGT GTCCATTGGG CCAGTGCAAA GTCTCCATCA TACCTCCAAC GGCACTCAAC 
AGTATCGACT CTGATGGGTT TTGGAAAGCA ACATGCCCAC CTTCCTGTGC CAGCCCACTT 
CTAGTTTTTG TCAATTCCAA AAGCGGAGAC AATCAGGGGG TCAAGTTTCT ACGGAGGTTC 
AAACAGTTAC TTAATCCTGC TCAGGTGTTC GACTTAATGA ATGGAGGACC CCATCTAGGT 
TTACGTTTGT TTCAGAAGTT TGACAATTTC CGGATCCTTG TTTGTGGTGG AGATGGAAGT 
GTTGGTTGGG TTTTATCGGA GATTGATAAG CTAAGTTTAC ATAAACAGTG TCAACTGGGC 
GTACTTCCAC TGGGTACTGG AAATGACTTG GCTCGTGTGC TAGGTTGGGG TGCTTCTTGT 
GATGATGATA CTCAGCTGCC TCAAATTCTG GAAAAGTTGG AACGTGCTAG CACAAAAATG 
CTGGATAGAT GGAGTATTAT GTCATATGAA CTTAAATTGC CAACAAAGCC TTCTATATCT 
CCTTTATCTC CAGAGGAGGA ATCTGAAGAG TGTCAATTTC AGATATCTGC ATATGAGGAT 
TCAGTGGCTA CTCATCTTGC AAAGATTCTA AATTCAGACA AGCATTCAGT AGTGATTTCC 
TCTGCTAAGG TTTTATGTGA AACTGTAAAG GACTTTGTTG CAAAAGTCGG GAAGGCTTAT 
GAGAAACCAA TGGAAAATGC AGAGGAAGCT GAATCAATGG CTGTAAAGTG TTCAACTCTG 
AATGAAAAAT TGGACCTGCT GCTTCAGACT CTGCATGCAG AATCTCAGGC TGCTCCATTG 
CTGGCACATT CCACCCCCCC TATTGTGGAA GAAGAAGTTG AGGAAGAGTT TTCCAGTGAA 
GAATCCCTGT CTGAAAGTAG AGAACAGTTA GCTGACAGCA TACCAAAGTC TTCCAGCCAA 
AGGCTCTTCA AACCAAGAGA GCAACTCATG CTGCGGGCTA ACAGCCTGAA AAAAGCTGTC 
AGGCAGATAA TAGAGCAAGC TGAGAAAGTG GTAGATGAGC AGAATGCACA CACTGAGGAA 
CAAGAAAACC AGTCTCCTGA GGAATACAGA AAGGAGTTGG AGGAATCTAA AGAGGAAGAA 
AAAGATGAAG ACACAAAAGA ATCTGAATTT CAGGAGTCTG GCAAGGTTCC CCCACGGTCA 
CCAGACAGAC AGACAAGCCG CAGTACATAT ACTCACACAG GCTCATTCTC AGGGCCACCC 
TTGGCATCAA GCAAGGAAAA CCTACCTGTG TTGAACACTA GAATAATCTG TCCAGGTCTG 
AGGGCAGGGT TGGCGGCCTC CATCGCTGGG AGTTCTATCA TCAATAAAAT GTTACTGGCA 
AACATAGATC CACTGGGGGC TACTCCATTT ATTGACCCTG ATCCAGATTC ACTGGAAGGC 
TATTCAGAAA AATGTGTAAT GAACAACTAT TTTGGAATTG GCCTGGATGC AAAGATATCC 
TTAGAATTTA ACAACAAGCG AGAAGAACAC CCTGAAAAAT GCAGAAGCCG AACTAAAAAT 
ATGATGTGGT ATGGTGTGCT TGGCACCAAG GAACTTCTGC AGCGAACATA CAAAAACTTA 
GAGCAGAAAG TCCAGCTTGA GTGTGATGGG CAGTACATCC CTCTTCCCAG CCTTCAGGGT 
ATTGCTGTGC TGAACATTCC AAGCTATGCT GGCGGCACTA ACTTCTGGGG TGGAACCAAG 
GAAGATGATA TCTTTGGTGC GCCGTCATTT GATGATAAAA TCTTGGAGGT GGTAGCAGTT 
TTTGGAAGTA TGCAGATGGC AGTATCTAGG GTGATAAAGC TGCAACATCA TAGAATAGCA 
CAGTGCCGGT CAGTGAAGAT CACCATACTT GGAGAAGAGG GAGTCCCTGT TCAGGTGGAT 
GGAGAGGCTT GGATTCAGCC ACCTGGCGTT ATTAAAATTG TACATAAAAA CCGAGCTCAG 
ATGCTGACTA GGGACAGGGC TTTTGAGAAC ACCCTGAAGT CCTGGGAAGA TAAGCAGAAG 
TACGATTCCT GTAAGCTTCC CCTTCGCTCT CATCTCTATT CTCAACAAGC TGTTGATTTG 
GCTTCAGAAG AAGAAGTCAC ACTAATCCAT CTTTGCTCGA AAGCTTCAGA GGAGCTTATT 
ACAAGAATAT GCGAAGCTGC AAAAATCCAT AGCTTGCTGG AACAAGAACT GGCTCATGCG 
GTGAATGCCT GCTCTCATGC ACTAAATAAA GCCAATCCCC GGTTTCCCGA GAGCCTAACA 
AGAAACACTG CCATTGAGGT AGCAGTAACT GCAAAAGCTT TGCACAATGA AACAGAATCT 
CTCTTGGTTG GAAGAGTGCC CCTGCAGTTG GAATCCCATC ATGAAGAACT TCTATCCAAT 
GCCCTTCACA GTGTAGAAGT TGAGCTAACA AAACTGGCTG AGATTCCTTG GCTGTACTTT 
GTCTTACAGC CAGATGAGGA GGATCCTCCT CTGGAATACT CTAAAAGAAA CAGCAGAAGC 
ACAGTATTCC GAATAGTGCC AAAGTTTAAA AGGGAGAAGG CCTTAAAGAA TAAGCCCAGC 
ATTCAGCCTG TTCAAAAATG GGGCACAGAT GAAGTGGCAG TTTGGCTGGA TCTTCTCAGT 
TTGGGAGAGT ACAGAGAAAT CTTCATCCGT CATGATATCC GTGGCTCAGA GCTATTGCAC 
CTGGAGAGGC GGGACCTTAA GGATCTGGGG ATATTGAAAG TGGGTCATAT GAAGCGAATT 
CTACAGGGAA TTAAAGAGCT CAGCAAAAAC TCCTCTTTAT CGGACATGTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004911891.1
CDS	178..3888
Translation	MMETACPSYSRRSPVWEEMQECDKMSGSMREPAVAPGAPAGDESSDSEGEHEGPQKLIRKVSTSGQLRSKTSIKEGLLLKQTSSFQRWKKRYFKLRGRTLYYAKDSKSLIFDEVDLSDASVAESSTKNVNNSFTIITPFRRLILCAENRKEMEDWISSLKSVQSREHYETAQFNVEHFSGMHNWYACSHARPTFCNVCRESLSGVTSHGLSCEVCKFKAHKRCAVRATNNCKWTTLASIGKDIIEDEDGIAMPHQWLEGNLPVSAKCAVCDKTCGSVLRLQDWRCLWCKAMVHTACKDQYPRKCPLGQCKVSIIPPTALNSIDSDGFWKATCPPSCASPLLVFVNSKSGDNQGVKFLRRFKQLLNPAQVFDLMNGGPHLGLRLFQKFDNFRILVCGGDGSVGWVLSEIDKLSLHKQCQLGVLPLGTGNDLARVLGWGASCDDDTQLPQILEKLERASTKMLDRWSIMSYELKLPTKPSISPLSPEEESEECQFQISAYEDSVATHLAKILNSDKHSVVISSAKVLCETVKDFVAKVGKAYEKPMENAEEAESMAVKCSTLNEKLDLLLQTLHAESQAAPLLAHSTPPIVEEEVEEEFSSEESLSESREQLADSIPKSSSQRLFKPREQLMLRANSLKKAVRQIIEQAEKVVDEQNAHTEEQENQSPEEYRKELEESKEEEKDEDTKESEFQESGKVPPRSPDRQTSRSTYTHTGSFSGPPLASSKENLPVLNTRIICPGLRAGLAASIAGSSIINKMLLANIDPLGATPFIDPDPDSLEGYSEKCVMNNYFGIGLDAKISLEFNNKREEHPEKCRSRTKNMMWYGVLGTKELLQRTYKNLEQKVQLECDGQYIPLPSLQGIAVLNIPSYAGGTNFWGGTKEDDIFGAPSFDDKILEVVAVFGSMQMAVSRVIKLQHHRIAQCRSVKITILGEEGVPVQVDGEAWIQPPGVIKIVHKNRAQMLTRDRAFENTLKSWEDKQKYDSCKLPLRSHLYSQQAVDLASEEEVTLIHLCSKASEELITRICEAAKIHSLLEQELAHAVNACSHALNKANPRFPESLTRNTAIEVAVTAKALHNETESLLVGRVPLQLESHHEELLSNALHSVEVELTKLAEIPWLYFVLQPDEEDPPLEYSKRNSRSTVFRIVPKFKREKALKNKPSIQPVQKWGTDEVAVWLDLLSLGEYREIFIRHDIRGSELLHLERRDLKDLGILKVGHMKRILQGIKELSKNSSLSDM

Target ORF information:

RefSeq Version	XM_004911834.4
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis diacylglycerol kinase, eta (dgkh), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004911834.4

ORF Insert Sequence:

ATGATGGAGA CTGCCTGCCC CTCGTACAGC CGCCGGAGCC CAGTTTGGGA AGAGATGCAG 
GAGTGCGACA AGATGTCGGG TTCAATGAGG GAGCCTGCGG TGGCCCCTGG AGCTCCTGCG 
GGAGATGAGT CCTCTGACAG CGAGGGGGAG CATGAGGGGC CGCAGAAACT CATTCGCAAA 
GTGTCCACCT CTGGGCAACT CCGCAGCAAG ACAAGTATCA AAGAGGGATT ATTACTGAAG 
CAAACCAGTT CATTTCAGAG ATGGAAAAAA CGCTATTTTA AGCTACGTGG CAGAACCTTG 
TACTATGCCA AGGATTCAAA GTCTCTAATA TTTGATGAAG TTGACCTGTC AGATGCCAGT 
GTAGCTGAAT CAAGCACAAA AAATGTCAAC AACAGCTTCA CGATAATCAC CCCTTTCAGA 
AGGCTTATAT TGTGTGCTGA GAACAGAAAA GAAATGGAGG ATTGGATCAG TTCACTAAAG 
TCTGTCCAGT CAAGAGAGCA TTATGAGACC GCACAGTTTA ATGTGGAACA TTTCTCAGGG 
ATGCATAACT GGTATGCATG TTCCCATGCC CGACCCACCT TCTGTAATGT GTGCAGAGAA 
AGCCTTTCTG GGGTTACCTC ACATGGCCTT TCTTGTGAAG TCTGCAAATT CAAAGCTCAT 
AAAAGATGTG CAGTTAGAGC AACAAACAAC TGCAAGTGGA CCACCCTGGC TTCAATCGGC 
AAAGATATTA TTGAGGATGA GGATGGTATA GCAATGCCTC ACCAGTGGCT AGAGGGTAAT 
CTGCCTGTTA GTGCCAAATG TGCTGTTTGT GACAAAACCT GTGGCAGCGT ATTACGTCTG 
CAGGACTGGA GGTGCCTTTG GTGTAAAGCA ATGGTGCACA CAGCATGCAA GGACCAGTAT 
CCTCGAAAGT GTCCATTGGG CCAGTGCAAA GTCTCCATCA TACCTCCAAC GGCACTCAAC 
AGTATCGACT CTGATGGGTT TTGGAAAGCA ACATGCCCAC CTTCCTGTGC CAGCCCACTT 
CTAGTTTTTG TCAATTCCAA AAGCGGAGAC AATCAGGGGG TCAAGTTTCT ACGGAGGTTC 
AAACAGTTAC TTAATCCTGC TCAGGTGTTC GACTTAATGA ATGGAGGACC CCATCTAGGT 
TTACGTTTGT TTCAGAAGTT TGACAATTTC CGGATCCTTG TTTGTGGTGG AGATGGAAGT 
GTTGGTTGGG TTTTATCGGA GATTGATAAG CTAAGTTTAC ATAAACAGTG TCAACTGGGC 
GTACTTCCAC TGGGTACTGG AAATGACTTG GCTCGTGTGC TAGGTTGGGG TGCTTCTTGT 
GATGATGATA CTCAGCTGCC TCAAATTCTG GAAAAGTTGG AACGTGCTAG CACAAAAATG 
CTGGATAGAT GGAGTATTAT GTCATATGAA CTTAAATTGC CAACAAAGCC TTCTATATCT 
CCTTTATCTC CAGAGGAGGA ATCTGAAGAG TGTCAATTTC AGATATCTGC ATATGAGGAT 
TCAGTGGCTA CTCATCTTGC AAAGATTCTA AATTCAGACA AGCATTCAGT AGTGATTTCC 
TCTGCTAAGG TTTTATGTGA AACTGTAAAG GACTTTGTTG CAAAAGTCGG GAAGGCTTAT 
GAGAAACCAA TGGAAAATGC AGAGGAAGCT GAATCAATGG CTGTAAAGTG TTCAACTCTG 
AATGAAAAAT TGGACCTGCT GCTTCAGACT CTGCATGCAG AATCTCAGGC TGCTCCATTG 
CTGGCACATT CCACCCCCCC TATTGTGGAA GAAGAAGTTG AGGAAGAGTT TTCCAGTGAA 
GAATCCCTGT CTGAAAGTAG AGAACAGTTA GCTGACAGCA TACCAAAGTC TTCCAGCCAA 
AGGCTCTTCA AACCAAGAGA GCAACTCATG CTGCGGGCTA ACAGCCTGAA AAAAGCTGTC 
AGGCAGATAA TAGAGCAAGC TGAGAAAGTG GTAGATGAGC AGAATGCACA CACTGAGGAA 
CAAGAAAACC AGTCTCCTGA GGAATACAGA AAGGAGTTGG AGGAATCTAA AGAGGAAGAA 
AAAGATGAAG ACACAAAAGA ATCTGAATTT CAGGAGTCTG GCAAGGTTCC CCCACGGTCA 
CCAGACAGAC AGACAAGCCG CAGTACATAT ACTCACACAG GCTCATTCTC AGGGCCACCC 
TTGGCATCAA GCAAGGAAAA CCTACCTGTG TTGAACACTA GAATAATCTG TCCAGGTCTG 
AGGGCAGGGT TGGCGGCCTC CATCGCTGGG AGTTCTATCA TCAATAAAAT GTTACTGGCA 
AACATAGATC CACTGGGGGC TACTCCATTT ATTGACCCTG ATCCAGATTC ACTGGAAGGC 
TATTCAGAAA AATGTGTAAT GAACAACTAT TTTGGAATTG GCCTGGATGC AAAGATATCC 
TTAGAATTTA ACAACAAGCG AGAAGAACAC CCTGAAAAAT GCAGAAGCCG AACTAAAAAT 
ATGATGTGGT ATGGTGTGCT TGGCACCAAG GAACTTCTGC AGCGAACATA CAAAAACTTA 
GAGCAGAAAG TCCAGCTTGA GTGTGATGGG CAGTACATCC CTCTTCCCAG CCTTCAGGGT 
ATTGCTGTGC TGAACATTCC AAGCTATGCT GGCGGCACTA ACTTCTGGGG TGGAACCAAG 
GAAGATGATA TCTTTGGTGC GCCGTCATTT GATGATAAAA TCTTGGAGGT GGTAGCAGTT 
TTTGGAAGTA TGCAGATGGC AGTATCTAGG GTGATAAAGC TGCAACATCA TAGAATAGCA 
CAGTGCCGGT CAGTGAAGAT CACCATACTT GGAGAAGAGG GAGTCCCTGT TCAGGTGGAT 
GGAGAGGCTT GGATTCAGCC ACCTGGCGTT ATTAAAATTG TACATAAAAA CCGAGCTCAG 
ATGCTGACTA GGGACAGGGC TTTTGAGAAC ACCCTGAAGT CCTGGGAAGA TAAGCAGAAG 
TACGATTCCT GTAAGCTTCC CCTTCGCTCT CATCTCTATT CTCAACAAGC TGTTGATTTG 
GCTTCAGAAG AAGAAGTCAC ACTAATCCAT CTTTGCTCGA AAGCTTCAGA GGAGCTTATT 
ACAAGAATAT GCGAAGCTGC AAAAATCCAT AGCTTGCTGG AACAAGAACT GGCTCATGCG 
GTGAATGCCT GCTCTCATGC ACTAAATAAA GCCAATCCCC GGTTTCCCGA GAGCCTAACA 
AGAAACACTG CCATTGAGGT AGCAGTAACT GCAAAAGCTT TGCACAATGA AACAGAATCT 
CTCTTGGTTG GAAGAGTGCC CCTGCAGTTG GAATCCCATC ATGAAGAACT TCTATCCAAT 
GCCCTTCACA GTGTAGAAGT TGAGCTAACA AAACTGGCTG AGATTCCTTG GCTGTACTTT 
GTCTTACAGC CAGATGAGGA GGATCCTCCT CTGGAATACT CTAAAAGAAA CAGCAGAAGC 
ACAGTATTCC GAATAGTGCC AAAGTTTAAA AGGGAGAAGG CCTTAAAGAA TAAGCCCAGC 
ATTCAGCCTG TTCAAAAATG GGGCACAGAT GAAGTGGCAG TTTGGCTGGA TCTTCTCAGT 
TTGGGAGAGT ACAGAGAAAT CTTCATCCGT CATGATATCC GTGGCTCAGA GCTATTGCAC 
CTGGAGAGGC GGGACCTTAA GGATCTGGGG ATATTGAAAG TGGGTCATAT GAAGCGAATT 
CTACAGGGAA TTAAAGAGCT CAGCAAAAAC TCCTCTTTAT CGGACATGTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

dgkh ( XM_012957746.3 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012813200.1 Xenopus tropicalis diacylglycerol kinase, eta (dgkh), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OXa52619
Clone ID Related Accession (Same CDS sequence)	XM_012957746.3
Accession Version	XM_012957746.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3708bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1576512000000
Organism	Xenopus tropicalis(tropical clawed frog)
Product	diacylglycerol kinase eta isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030678.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012957746.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGGAGA CTGCCTGCCC CTCGTACAGC CGCCGGAGCC CAGTTTGGGA AGAGATGGAG 
TGCGACAAGA TGTCGGGTTC AATGAGGGAG CCTGCGGTGG CCCCTGGAGC TCCTGCGGGA 
GATGAGTCCT CTGACAGCGA GGGGGAGCAT GAGGGGCCGC AGAAACTCAT TCGCAAAGTG 
TCCACCTCTG GGCAACTCCG CAGCAAGACA AGTATCAAAG AGGGATTATT ACTGAAGCAA 
ACCAGTTCAT TTCAGAGATG GAAAAAACGC TATTTTAAGC TACGTGGCAG AACCTTGTAC 
TATGCCAAGG ATTCAAAGTC TCTAATATTT GATGAAGTTG ACCTGTCAGA TGCCAGTGTA 
GCTGAATCAA GCACAAAAAA TGTCAACAAC AGCTTCACGA TAATCACCCC TTTCAGAAGG 
CTTATATTGT GTGCTGAGAA CAGAAAAGAA ATGGAGGATT GGATCAGTTC ACTAAAGTCT 
GTCCAGTCAA GAGAGCATTA TGAGACCGCA CAGTTTAATG TGGAACATTT CTCAGGGATG 
CATAACTGGT ATGCATGTTC CCATGCCCGA CCCACCTTCT GTAATGTGTG CAGAGAAAGC 
CTTTCTGGGG TTACCTCACA TGGCCTTTCT TGTGAAGTCT GCAAATTCAA AGCTCATAAA 
AGATGTGCAG TTAGAGCAAC AAACAACTGC AAGTGGACCA CCCTGGCTTC AATCGGCAAA 
GATATTATTG AGGATGAGGA TGGTATAGCA ATGCCTCACC AGTGGCTAGA GGGTAATCTG 
CCTGTTAGTG CCAAATGTGC TGTTTGTGAC AAAACCTGTG GCAGCGTATT ACGTCTGCAG 
GACTGGAGGT GCCTTTGGTG TAAAGCAATG GTGCACACAG CATGCAAGGA CCAGTATCCT 
CGAAAGTGTC CATTGGGCCA GTGCAAAGTC TCCATCATAC CTCCAACGGC ACTCAACAGT 
ATCGACTCTG ATGGGTTTTG GAAAGCAACA TGCCCACCTT CCTGTGCCAG CCCACTTCTA 
GTTTTTGTCA ATTCCAAAAG CGGAGACAAT CAGGGGGTCA AGTTTCTACG GAGGTTCAAA 
CAGTTACTTA ATCCTGCTCA GGTGTTCGAC TTAATGAATG GAGGACCCCA TCTAGGTTTA 
CGTTTGTTTC AGAAGTTTGA CAATTTCCGG ATCCTTGTTT GTGGTGGAGA TGGAAGTGTT 
GGTTGGGTTT TATCGGAGAT TGATAAGCTA AGTTTACATA AACAGTGTCA ACTGGGCGTA 
CTTCCACTGG GTACTGGAAA TGACTTGGCT CGTGTGCTAG GTTGGGGTGC TTCTTGTGAT 
GATGATACTC AGCTGCCTCA AATTCTGGAA AAGTTGGAAC GTGCTAGCAC AAAAATGCTG 
GATAGATGGA GTATTATGTC ATATGAACTT AAATTGCCAA CAAAGCCTTC TATATCTCCT 
TTATCTCCAG AGGAGGAATC TGAAGAGTGT CAATTTCAGA TATCTGCATA TGAGGATTCA 
GTGGCTACTC ATCTTGCAAA GATTCTAAAT TCAGACAAGC ATTCAGTAGT GATTTCCTCT 
GCTAAGGTTT TATGTGAAAC TGTAAAGGAC TTTGTTGCAA AAGTCGGGAA GGCTTATGAG 
AAACCAATGG AAAATGCAGA GGAAGCTGAA TCAATGGCTG TAAAGTGTTC AACTCTGAAT 
GAAAAATTGG ACCTGCTGCT TCAGACTCTG CATGCAGAAT CTCAGGCTGC TCCATTGCTG 
GCACATTCCA CCCCCCCTAT TGTGGAAGAA GAAGTTGAGG AAGAGTTTTC CAGTGAAGAA 
TCCCTGTCTG AAAGTAGAGA ACAGTTAGCT GACAGCATAC CAAAGTCTTC CAGCCAAAGG 
CTCTTCAAAC CAAGAGAGCA ACTCATGCTG CGGGCTAACA GCCTGAAAAA AGCTGTCAGG 
CAGATAATAG AGCAAGCTGA GAAAGTGGTA GATGAGCAGA ATGCACACAC TGAGGAACAA 
GAAAACCAGT CTCCTGAGGA ATACAGAAAG GAGTTGGAGG AATCTAAAGA GGAAGAAAAA 
GATGAAGACA CAAAAGAATC TGAATTTCAG GAGTCTGGCA AGGTTCCCCC ACGGTCACCA 
GACAGACAGA CAAGCCGCAG TACATATACT CACACAGGCT CATTCTCAGG GCCACCCTTG 
GCATCAAGCA AGGAAAACCT ACCTGTGTTG AACACTAGAA TAATCTGTCC AGGTCTGAGG 
GCAGGGTTGG CGGCCTCCAT CGCTGGGAGT TCTATCATCA ATAAAATGTT ACTGGCAAAC 
ATAGATCCAC TGGGGGCTAC TCCATTTATT GACCCTGATC CAGATTCACT GGAAGGCTAT 
TCAGAAAAAT GTGTAATGAA CAACTATTTT GGAATTGGCC TGGATGCAAA GATATCCTTA 
GAATTTAACA ACAAGCGAGA AGAACACCCT GAAAAATGCA GAAGCCGAAC TAAAAATATG 
ATGTGGTATG GTGTGCTTGG CACCAAGGAA CTTCTGCAGC GAACATACAA AAACTTAGAG 
CAGAAAGTCC AGCTTGAGTG TGATGGGCAG TACATCCCTC TTCCCAGCCT TCAGGGTATT 
GCTGTGCTGA ACATTCCAAG CTATGCTGGC GGCACTAACT TCTGGGGTGG AACCAAGGAA 
GATGATATCT TTGGTGCGCC GTCATTTGAT GATAAAATCT TGGAGGTGGT AGCAGTTTTT 
GGAAGTATGC AGATGGCAGT ATCTAGGGTG ATAAAGCTGC AACATCATAG AATAGCACAG 
TGCCGGTCAG TGAAGATCAC CATACTTGGA GAAGAGGGAG TCCCTGTTCA GGTGGATGGA 
GAGGCTTGGA TTCAGCCACC TGGCGTTATT AAAATTGTAC ATAAAAACCG AGCTCAGATG 
CTGACTAGGG ACAGGGCTTT TGAGAACACC CTGAAGTCCT GGGAAGATAA GCAGAAGTAC 
GATTCCTGTA AGCTTCCCCT TCGCTCTCAT CTCTATTCTC AACAAGCTGT TGATTTGGCT 
TCAGAAGAAG AAGTCACACT AATCCATCTT TGCTCGAAAG CTTCAGAGGA GCTTATTACA 
AGAATATGCG AAGCTGCAAA AATCCATAGC TTGCTGGAAC AAGAACTGGC TCATGCGGTG 
AATGCCTGCT CTCATGCACT AAATAAAGCC AATCCCCGGT TTCCCGAGAG CCTAACAAGA 
AACACTGCCA TTGAGGTAGC AGTAACTGCA AAAGCTTTGC ACAATGAAAC AGAATCTCTC 
TTGGTTGGAA GAGTGCCCCT GCAGTTGGAA TCCCATCATG AAGAACTTCT ATCCAATGCC 
CTTCACAGTG TAGAAGTTGA GCTAACAAAA CTGGCTGAGA TTCCTTGGCT GTACTTTGTC 
TTACAGCCAG ATGAGGAGGA TCCTCCTCTG GAATACTCTA AAAGAAACAG CAGAAGCACA 
GTATTCCGAA TAGTGCCAAA GTTTAAAAGG GAGAAGGCCT TAAAGAATAA GCCCAGCATT 
CAGCCTGTTC AAAAATGGGG CACAGATGAA GTGGCAGTTT GGCTGGATCT TCTCAGTTTG 
GGAGAGTACA GAGAAATCTT CATCCGTCAT GATATCCGTG GCTCAGAGCT ATTGCACCTG 
GAGAGGCGGG ACCTTAAGGA TCTGGGGATA TTGAAAGTGG GTCATATGAA GCGAATTCTA 
CAGGGAATTA AAGAGCTCAG CAAAAACTCC TCTTTATCGG ACATGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012813200.1
CDS	176..3883
Translation	MMETACPSYSRRSPVWEEMECDKMSGSMREPAVAPGAPAGDESSDSEGEHEGPQKLIRKVSTSGQLRSKTSIKEGLLLKQTSSFQRWKKRYFKLRGRTLYYAKDSKSLIFDEVDLSDASVAESSTKNVNNSFTIITPFRRLILCAENRKEMEDWISSLKSVQSREHYETAQFNVEHFSGMHNWYACSHARPTFCNVCRESLSGVTSHGLSCEVCKFKAHKRCAVRATNNCKWTTLASIGKDIIEDEDGIAMPHQWLEGNLPVSAKCAVCDKTCGSVLRLQDWRCLWCKAMVHTACKDQYPRKCPLGQCKVSIIPPTALNSIDSDGFWKATCPPSCASPLLVFVNSKSGDNQGVKFLRRFKQLLNPAQVFDLMNGGPHLGLRLFQKFDNFRILVCGGDGSVGWVLSEIDKLSLHKQCQLGVLPLGTGNDLARVLGWGASCDDDTQLPQILEKLERASTKMLDRWSIMSYELKLPTKPSISPLSPEEESEECQFQISAYEDSVATHLAKILNSDKHSVVISSAKVLCETVKDFVAKVGKAYEKPMENAEEAESMAVKCSTLNEKLDLLLQTLHAESQAAPLLAHSTPPIVEEEVEEEFSSEESLSESREQLADSIPKSSSQRLFKPREQLMLRANSLKKAVRQIIEQAEKVVDEQNAHTEEQENQSPEEYRKELEESKEEEKDEDTKESEFQESGKVPPRSPDRQTSRSTYTHTGSFSGPPLASSKENLPVLNTRIICPGLRAGLAASIAGSSIINKMLLANIDPLGATPFIDPDPDSLEGYSEKCVMNNYFGIGLDAKISLEFNNKREEHPEKCRSRTKNMMWYGVLGTKELLQRTYKNLEQKVQLECDGQYIPLPSLQGIAVLNIPSYAGGTNFWGGTKEDDIFGAPSFDDKILEVVAVFGSMQMAVSRVIKLQHHRIAQCRSVKITILGEEGVPVQVDGEAWIQPPGVIKIVHKNRAQMLTRDRAFENTLKSWEDKQKYDSCKLPLRSHLYSQQAVDLASEEEVTLIHLCSKASEELITRICEAAKIHSLLEQELAHAVNACSHALNKANPRFPESLTRNTAIEVAVTAKALHNETESLLVGRVPLQLESHHEELLSNALHSVEVELTKLAEIPWLYFVLQPDEEDPPLEYSKRNSRSTVFRIVPKFKREKALKNKPSIQPVQKWGTDEVAVWLDLLSLGEYREIFIRHDIRGSELLHLERRDLKDLGILKVGHMKRILQGIKELSKNSSLSDM

Target ORF information:

RefSeq Version	XM_012957746.3
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis diacylglycerol kinase, eta (dgkh), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012957746.3

ORF Insert Sequence:

ATGATGGAGA CTGCCTGCCC CTCGTACAGC CGCCGGAGCC CAGTTTGGGA AGAGATGGAG 
TGCGACAAGA TGTCGGGTTC AATGAGGGAG CCTGCGGTGG CCCCTGGAGC TCCTGCGGGA 
GATGAGTCCT CTGACAGCGA GGGGGAGCAT GAGGGGCCGC AGAAACTCAT TCGCAAAGTG 
TCCACCTCTG GGCAACTCCG CAGCAAGACA AGTATCAAAG AGGGATTATT ACTGAAGCAA 
ACCAGTTCAT TTCAGAGATG GAAAAAACGC TATTTTAAGC TACGTGGCAG AACCTTGTAC 
TATGCCAAGG ATTCAAAGTC TCTAATATTT GATGAAGTTG ACCTGTCAGA TGCCAGTGTA 
GCTGAATCAA GCACAAAAAA TGTCAACAAC AGCTTCACGA TAATCACCCC TTTCAGAAGG 
CTTATATTGT GTGCTGAGAA CAGAAAAGAA ATGGAGGATT GGATCAGTTC ACTAAAGTCT 
GTCCAGTCAA GAGAGCATTA TGAGACCGCA CAGTTTAATG TGGAACATTT CTCAGGGATG 
CATAACTGGT ATGCATGTTC CCATGCCCGA CCCACCTTCT GTAATGTGTG CAGAGAAAGC 
CTTTCTGGGG TTACCTCACA TGGCCTTTCT TGTGAAGTCT GCAAATTCAA AGCTCATAAA 
AGATGTGCAG TTAGAGCAAC AAACAACTGC AAGTGGACCA CCCTGGCTTC AATCGGCAAA 
GATATTATTG AGGATGAGGA TGGTATAGCA ATGCCTCACC AGTGGCTAGA GGGTAATCTG 
CCTGTTAGTG CCAAATGTGC TGTTTGTGAC AAAACCTGTG GCAGCGTATT ACGTCTGCAG 
GACTGGAGGT GCCTTTGGTG TAAAGCAATG GTGCACACAG CATGCAAGGA CCAGTATCCT 
CGAAAGTGTC CATTGGGCCA GTGCAAAGTC TCCATCATAC CTCCAACGGC ACTCAACAGT 
ATCGACTCTG ATGGGTTTTG GAAAGCAACA TGCCCACCTT CCTGTGCCAG CCCACTTCTA 
GTTTTTGTCA ATTCCAAAAG CGGAGACAAT CAGGGGGTCA AGTTTCTACG GAGGTTCAAA 
CAGTTACTTA ATCCTGCTCA GGTGTTCGAC TTAATGAATG GAGGACCCCA TCTAGGTTTA 
CGTTTGTTTC AGAAGTTTGA CAATTTCCGG ATCCTTGTTT GTGGTGGAGA TGGAAGTGTT 
GGTTGGGTTT TATCGGAGAT TGATAAGCTA AGTTTACATA AACAGTGTCA ACTGGGCGTA 
CTTCCACTGG GTACTGGAAA TGACTTGGCT CGTGTGCTAG GTTGGGGTGC TTCTTGTGAT 
GATGATACTC AGCTGCCTCA AATTCTGGAA AAGTTGGAAC GTGCTAGCAC AAAAATGCTG 
GATAGATGGA GTATTATGTC ATATGAACTT AAATTGCCAA CAAAGCCTTC TATATCTCCT 
TTATCTCCAG AGGAGGAATC TGAAGAGTGT CAATTTCAGA TATCTGCATA TGAGGATTCA 
GTGGCTACTC ATCTTGCAAA GATTCTAAAT TCAGACAAGC ATTCAGTAGT GATTTCCTCT 
GCTAAGGTTT TATGTGAAAC TGTAAAGGAC TTTGTTGCAA AAGTCGGGAA GGCTTATGAG 
AAACCAATGG AAAATGCAGA GGAAGCTGAA TCAATGGCTG TAAAGTGTTC AACTCTGAAT 
GAAAAATTGG ACCTGCTGCT TCAGACTCTG CATGCAGAAT CTCAGGCTGC TCCATTGCTG 
GCACATTCCA CCCCCCCTAT TGTGGAAGAA GAAGTTGAGG AAGAGTTTTC CAGTGAAGAA 
TCCCTGTCTG AAAGTAGAGA ACAGTTAGCT GACAGCATAC CAAAGTCTTC CAGCCAAAGG 
CTCTTCAAAC CAAGAGAGCA ACTCATGCTG CGGGCTAACA GCCTGAAAAA AGCTGTCAGG 
CAGATAATAG AGCAAGCTGA GAAAGTGGTA GATGAGCAGA ATGCACACAC TGAGGAACAA 
GAAAACCAGT CTCCTGAGGA ATACAGAAAG GAGTTGGAGG AATCTAAAGA GGAAGAAAAA 
GATGAAGACA CAAAAGAATC TGAATTTCAG GAGTCTGGCA AGGTTCCCCC ACGGTCACCA 
GACAGACAGA CAAGCCGCAG TACATATACT CACACAGGCT CATTCTCAGG GCCACCCTTG 
GCATCAAGCA AGGAAAACCT ACCTGTGTTG AACACTAGAA TAATCTGTCC AGGTCTGAGG 
GCAGGGTTGG CGGCCTCCAT CGCTGGGAGT TCTATCATCA ATAAAATGTT ACTGGCAAAC 
ATAGATCCAC TGGGGGCTAC TCCATTTATT GACCCTGATC CAGATTCACT GGAAGGCTAT 
TCAGAAAAAT GTGTAATGAA CAACTATTTT GGAATTGGCC TGGATGCAAA GATATCCTTA 
GAATTTAACA ACAAGCGAGA AGAACACCCT GAAAAATGCA GAAGCCGAAC TAAAAATATG 
ATGTGGTATG GTGTGCTTGG CACCAAGGAA CTTCTGCAGC GAACATACAA AAACTTAGAG 
CAGAAAGTCC AGCTTGAGTG TGATGGGCAG TACATCCCTC TTCCCAGCCT TCAGGGTATT 
GCTGTGCTGA ACATTCCAAG CTATGCTGGC GGCACTAACT TCTGGGGTGG AACCAAGGAA 
GATGATATCT TTGGTGCGCC GTCATTTGAT GATAAAATCT TGGAGGTGGT AGCAGTTTTT 
GGAAGTATGC AGATGGCAGT ATCTAGGGTG ATAAAGCTGC AACATCATAG AATAGCACAG 
TGCCGGTCAG TGAAGATCAC CATACTTGGA GAAGAGGGAG TCCCTGTTCA GGTGGATGGA 
GAGGCTTGGA TTCAGCCACC TGGCGTTATT AAAATTGTAC ATAAAAACCG AGCTCAGATG 
CTGACTAGGG ACAGGGCTTT TGAGAACACC CTGAAGTCCT GGGAAGATAA GCAGAAGTAC 
GATTCCTGTA AGCTTCCCCT TCGCTCTCAT CTCTATTCTC AACAAGCTGT TGATTTGGCT 
TCAGAAGAAG AAGTCACACT AATCCATCTT TGCTCGAAAG CTTCAGAGGA GCTTATTACA 
AGAATATGCG AAGCTGCAAA AATCCATAGC TTGCTGGAAC AAGAACTGGC TCATGCGGTG 
AATGCCTGCT CTCATGCACT AAATAAAGCC AATCCCCGGT TTCCCGAGAG CCTAACAAGA 
AACACTGCCA TTGAGGTAGC AGTAACTGCA AAAGCTTTGC ACAATGAAAC AGAATCTCTC 
TTGGTTGGAA GAGTGCCCCT GCAGTTGGAA TCCCATCATG AAGAACTTCT ATCCAATGCC 
CTTCACAGTG TAGAAGTTGA GCTAACAAAA CTGGCTGAGA TTCCTTGGCT GTACTTTGTC 
TTACAGCCAG ATGAGGAGGA TCCTCCTCTG GAATACTCTA AAAGAAACAG CAGAAGCACA 
GTATTCCGAA TAGTGCCAAA GTTTAAAAGG GAGAAGGCCT TAAAGAATAA GCCCAGCATT 
CAGCCTGTTC AAAAATGGGG CACAGATGAA GTGGCAGTTT GGCTGGATCT TCTCAGTTTG 
GGAGAGTACA GAGAAATCTT CATCCGTCAT GATATCCGTG GCTCAGAGCT ATTGCACCTG 
GAGAGGCGGG ACCTTAAGGA TCTGGGGATA TTGAAAGTGG GTCATATGAA GCGAATTCTA 
CAGGGAATTA AAGAGCTCAG CAAAAACTCC TCTTTATCGG ACATGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

dgkh ( XM_012957748.3 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012813202.1 Xenopus tropicalis diacylglycerol kinase, eta (dgkh), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OXa52620
Clone ID Related Accession (Same CDS sequence)	XM_012957748.3
Accession Version	XM_012957748.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3573bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1576512000000
Organism	Xenopus tropicalis(tropical clawed frog)
Product	diacylglycerol kinase eta isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030678.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012957748.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGCCA GGCGCTTCGC TGTGCCCCCG GCCGGTAAAA GGGGTGTAGG GAACCTGGCT 
GACACGGACT GCTTTGTTTT TTCCCAGCAG GAGTGCGACA AGATGTCGGG TTCAATGAGG 
GAGCCTGCGG TGGCCCCTGG AGCTCCTGCG GGAGATGAGT CCTCTGACAG CGAGGGGGAG 
CATGAGGGGC CGCAGAAACT CATTCGCAAA GTGTCCACCT CTGGGCAACT CCGCAGCAAG 
ACAAGTATCA AAGAGGGATT ATTACTGAAG CAAACCAGTT CATTTCAGAG ATGGAAAAAA 
CGCTATTTTA AGCTACGTGG CAGAACCTTG TACTATGCCA AGGATTCAAA GTCTCTAATA 
TTTGATGAAG TTGACCTGTC AGATGCCAGT GTAGCTGAAT CAAGCACAAA AAATGTCAAC 
AACAGCTTCA CGATAATCAC CCCTTTCAGA AGGCTTATAT TGTGTGCTGA GAACAGAAAA 
GAAATGGAGG ATTGGATCAG TTCACTAAAG TCTGTCCAGT CAAGAGAGCA TTATGAGACC 
GCACAGTTTA ATGTGGAACA TTTCTCAGGG ATGCATAACT GGTATGCATG TTCCCATGCC 
CGACCCACCT TCTGTAATGT GTGCAGAGAA AGCCTTTCTG GGGTTACCTC ACATGGCCTT 
TCTTGTGAAG TCTGCAAATT CAAAGCTCAT AAAAGATGTG CAGTTAGAGC AACAAACAAC 
TGCAAGTGGA CCACCCTGGC TTCAATCGGC AAAGATATTA TTGAGGATGA GGATGGTATA 
GCAATGCCTC ACCAGTGGCT AGAGGGTAAT CTGCCTGTTA GTGCCAAATG TGCTGTTTGT 
GACAAAACCT GTGGCAGCGT ATTACGTCTG CAGGACTGGA GGTGCCTTTG GTGTAAAGCA 
ATGGTGCACA CAGCATGCAA GGACCAGTAT CCTCGAAAGT GTCCATTGGG CCAGTGCAAA 
GTCTCCATCA TACCTCCAAC GGCACTCAAC AGTATCGACT CTGATGGGTT TTGGAAAGCA 
ACATGCCCAC CTTCCTGTGC CAGCCCACTT CTAGTTTTTG TCAATTCCAA AAGCGGAGAC 
AATCAGGGGG TCAAGTTTCT ACGGAGGTTC AAACAGTTAC TTAATCCTGC TCAGGTGTTC 
GACTTAATGA ATGGAGGACC CCATCTAGGT TTACGTTTGT TTCAGAAGTT TGACAATTTC 
CGGATCCTTG TTTGTGGTGG AGATGGAAGT GTTGGTTGGG TTTTATCGGA GATTGATAAG 
CTAAGTTTAC ATAAACAGTG TCAACTGGGC GTACTTCCAC TGGGTACTGG AAATGACTTG 
GCTCGTGTGC TAGGTTGGGG TGCTTCTTGT GATGATGATA CTCAGCTGCC TCAAATTCTG 
GAAAAGTTGG AACGTGCTAG CACAAAAATG CTGGATAGAT GGAGTATTAT GTCATATGAA 
CTTAAATTGC CAACAAAGCC TTCTATATCT CCTTTATCTC CAGAGGAGGA ATCTGAAGAG 
TGTCAATTTC AGATATCTGC ATATGAGGAT TCAGTGGCTA CTCATCTTGC AAAGATTCTA 
AATTCAGACA AGCATTCAGT AGTGATTTCC TCTGCTAAGG TTTTATGTGA AACTGTAAAG 
GACTTTGTTG CAAAAGTCGG GAAGGCTTAT GAGAAACCAA TGGAAAATGC AGAGGAAGCT 
GAATCAATGG CTGTAAAGTG TTCAACTCTG AATGAAAAAT TGGACCTGCT GCTTCAGACT 
CTGCATGCAG AATCTCAGGC TGCTCCATTG CTGGCACATT CCACCCCCCC TATTGTGGAA 
GAAGAAGTTG AGGAAGAGTT TTCCAGTGAA GAATCCCTGT CTGAAAGTAG AGAACAGTTA 
GCTGACAGCA TACCAAAGTC TTCCAGCCAA AGGCTCTTCA AACCAAGAGA GCAACTCATG 
CTGCGGGCTA ACAGCCTGAA AAAAGCTGTC AGGCAGATAA TAGAGCAAGC TGAGAAAGTG 
GTAGATGAGC AGAATGCACA CACTGAGGAA CAAGAAAACC AGTCTCCTGA GGAATACAGA 
AAGGAGTTGG AGGAATCTAA AGAGGAAGAA AAAGATGAAG ACACAAAAGA ATCTGAATTT 
CAGGAGTCTG GCAAGGTTCC CCCACGGTCA CCAGACAGAC AGACAAGCCG CAGTACATAT 
ACTCACACAG GCTCATTCTC AGGGCCACCC TTGGCATCAA GCAAGGAAAA CCTACCTGTG 
TTGAACACTA GAATAATCTG TCCAGGTCTG AGGGCAGGGT TGGCGGCCTC CATCGCTGGG 
AGTTCTATCA TCAATAAAAT GTTACTGGCA AACATAGATC CACTGGGGGC TACTCCATTT 
ATTGACCCTG ATCCAGATTC ACTGGAAGGC TATTCAGAAA AATGTGTAAT GAACAACTAT 
TTTGGAATTG GCCTGGATGC AAAGATATCC TTAGAATTTA ACAACAAGCG AGAAGAACAC 
CCTGAAAAAT GCAGAAGCCG AACTAAAAAT ATGATGTGGT ATGGTGTGCT TGGCACCAAG 
GAACTTCTGC AGCGAACATA CAAAAACTTA GAGCAGAAAG TCCAGCTTGA GTGTGATGGG 
CAGTACATCC CTCTTCCCAG CCTTCAGGGT ATTGCTGTGC TGAACATTCC AAGCTATGCT 
GGCGGCACTA ACTTCTGGGG TGGAACCAAG GAAGATGATA TCTTTGGTGC GCCGTCATTT 
GATGATAAAA TCTTGGAGGT GGTAGCAGTT TTTGGAAGTA TGCAGATGGC AGTATCTAGG 
GTGATAAAGC TGCAACATCA TAGAATAGCA CAGTGCCGGT CAGTGAAGAT CACCATACTT 
GGAGAAGAGG GAGTCCCTGT TCAGGTGGAT GGAGAGGCTT GGATTCAGCC ACCTGGCGTT 
ATTAAAATTG TACATAAAAA CCGAGCTCAG ATGCTGACTA GGGACAGGGC TTTTGAGAAC 
ACCCTGAAGT CCTGGGAAGA TAAGCAGAAG TACGATTCCT GTAAGCTTCC CCTTCGCTCT 
CATCTCTATT CTCAACAAGC TGTTGATTTG GCTTCAGAAG AAGAAGTCAC ACTAATCCAT 
CTTTGCTCGA AAGCTTCAGA GGAGCTTATT ACAAGAATAT GCGAAGCTGC AAAAATCCAT 
AGCTTGCTGG AACAAGAACT GGCTCATGCG GTGAATGCCT GCTCTCATGC ACTAAATAAA 
GCCAATCCCC GGTTTCCCGA GAGCCTAACA AGAAACACTG CCATTGAGGT AGCAGTAACT 
GCAAAAGCTT TGCACAATGA AACAGAATCT CTCTTGGTTG GAAGAGTGCC CCTGCAGTTG 
GAATCCCATC ATGAAGAACT TCTATCCAAT GCCCTTCACA GTGTAGAAGT TGAGCTAACA 
AAACTGGCTG AGATTCCTTG GCTGTACTTT GTCTTACAGC CAGATGAGGA GGATCCTCCT 
CTGGAATACT CTAAAAGAAA CAGCAGAAGC ACAGTATTCC GAATAGTGCC AAAGTTTAAA 
AGGGAGAAGG CCTTAAAGAA TAAGCCCAGC ATTCAGCCTG GATCTGGGGA TATTGAAAGT 
GGGTCATATG AAGCGAATTC TACAGGGAAT TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012813202.1
CDS	23..3595
Translation	MAARRFAVPPAGKRGVGNLADTDCFVFSQQECDKMSGSMREPAVAPGAPAGDESSDSEGEHEGPQKLIRKVSTSGQLRSKTSIKEGLLLKQTSSFQRWKKRYFKLRGRTLYYAKDSKSLIFDEVDLSDASVAESSTKNVNNSFTIITPFRRLILCAENRKEMEDWISSLKSVQSREHYETAQFNVEHFSGMHNWYACSHARPTFCNVCRESLSGVTSHGLSCEVCKFKAHKRCAVRATNNCKWTTLASIGKDIIEDEDGIAMPHQWLEGNLPVSAKCAVCDKTCGSVLRLQDWRCLWCKAMVHTACKDQYPRKCPLGQCKVSIIPPTALNSIDSDGFWKATCPPSCASPLLVFVNSKSGDNQGVKFLRRFKQLLNPAQVFDLMNGGPHLGLRLFQKFDNFRILVCGGDGSVGWVLSEIDKLSLHKQCQLGVLPLGTGNDLARVLGWGASCDDDTQLPQILEKLERASTKMLDRWSIMSYELKLPTKPSISPLSPEEESEECQFQISAYEDSVATHLAKILNSDKHSVVISSAKVLCETVKDFVAKVGKAYEKPMENAEEAESMAVKCSTLNEKLDLLLQTLHAESQAAPLLAHSTPPIVEEEVEEEFSSEESLSESREQLADSIPKSSSQRLFKPREQLMLRANSLKKAVRQIIEQAEKVVDEQNAHTEEQENQSPEEYRKELEESKEEEKDEDTKESEFQESGKVPPRSPDRQTSRSTYTHTGSFSGPPLASSKENLPVLNTRIICPGLRAGLAASIAGSSIINKMLLANIDPLGATPFIDPDPDSLEGYSEKCVMNNYFGIGLDAKISLEFNNKREEHPEKCRSRTKNMMWYGVLGTKELLQRTYKNLEQKVQLECDGQYIPLPSLQGIAVLNIPSYAGGTNFWGGTKEDDIFGAPSFDDKILEVVAVFGSMQMAVSRVIKLQHHRIAQCRSVKITILGEEGVPVQVDGEAWIQPPGVIKIVHKNRAQMLTRDRAFENTLKSWEDKQKYDSCKLPLRSHLYSQQAVDLASEEEVTLIHLCSKASEELITRICEAAKIHSLLEQELAHAVNACSHALNKANPRFPESLTRNTAIEVAVTAKALHNETESLLVGRVPLQLESHHEELLSNALHSVEVELTKLAEIPWLYFVLQPDEEDPPLEYSKRNSRSTVFRIVPKFKREKALKNKPSIQPGSGDIESGSYEANSTGN

Target ORF information:

RefSeq Version	XM_012957748.3
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis diacylglycerol kinase, eta (dgkh), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012957748.3

ORF Insert Sequence:

ATGGCGGCCA GGCGCTTCGC TGTGCCCCCG GCCGGTAAAA GGGGTGTAGG GAACCTGGCT 
GACACGGACT GCTTTGTTTT TTCCCAGCAG GAGTGCGACA AGATGTCGGG TTCAATGAGG 
GAGCCTGCGG TGGCCCCTGG AGCTCCTGCG GGAGATGAGT CCTCTGACAG CGAGGGGGAG 
CATGAGGGGC CGCAGAAACT CATTCGCAAA GTGTCCACCT CTGGGCAACT CCGCAGCAAG 
ACAAGTATCA AAGAGGGATT ATTACTGAAG CAAACCAGTT CATTTCAGAG ATGGAAAAAA 
CGCTATTTTA AGCTACGTGG CAGAACCTTG TACTATGCCA AGGATTCAAA GTCTCTAATA 
TTTGATGAAG TTGACCTGTC AGATGCCAGT GTAGCTGAAT CAAGCACAAA AAATGTCAAC 
AACAGCTTCA CGATAATCAC CCCTTTCAGA AGGCTTATAT TGTGTGCTGA GAACAGAAAA 
GAAATGGAGG ATTGGATCAG TTCACTAAAG TCTGTCCAGT CAAGAGAGCA TTATGAGACC 
GCACAGTTTA ATGTGGAACA TTTCTCAGGG ATGCATAACT GGTATGCATG TTCCCATGCC 
CGACCCACCT TCTGTAATGT GTGCAGAGAA AGCCTTTCTG GGGTTACCTC ACATGGCCTT 
TCTTGTGAAG TCTGCAAATT CAAAGCTCAT AAAAGATGTG CAGTTAGAGC AACAAACAAC 
TGCAAGTGGA CCACCCTGGC TTCAATCGGC AAAGATATTA TTGAGGATGA GGATGGTATA 
GCAATGCCTC ACCAGTGGCT AGAGGGTAAT CTGCCTGTTA GTGCCAAATG TGCTGTTTGT 
GACAAAACCT GTGGCAGCGT ATTACGTCTG CAGGACTGGA GGTGCCTTTG GTGTAAAGCA 
ATGGTGCACA CAGCATGCAA GGACCAGTAT CCTCGAAAGT GTCCATTGGG CCAGTGCAAA 
GTCTCCATCA TACCTCCAAC GGCACTCAAC AGTATCGACT CTGATGGGTT TTGGAAAGCA 
ACATGCCCAC CTTCCTGTGC CAGCCCACTT CTAGTTTTTG TCAATTCCAA AAGCGGAGAC 
AATCAGGGGG TCAAGTTTCT ACGGAGGTTC AAACAGTTAC TTAATCCTGC TCAGGTGTTC 
GACTTAATGA ATGGAGGACC CCATCTAGGT TTACGTTTGT TTCAGAAGTT TGACAATTTC 
CGGATCCTTG TTTGTGGTGG AGATGGAAGT GTTGGTTGGG TTTTATCGGA GATTGATAAG 
CTAAGTTTAC ATAAACAGTG TCAACTGGGC GTACTTCCAC TGGGTACTGG AAATGACTTG 
GCTCGTGTGC TAGGTTGGGG TGCTTCTTGT GATGATGATA CTCAGCTGCC TCAAATTCTG 
GAAAAGTTGG AACGTGCTAG CACAAAAATG CTGGATAGAT GGAGTATTAT GTCATATGAA 
CTTAAATTGC CAACAAAGCC TTCTATATCT CCTTTATCTC CAGAGGAGGA ATCTGAAGAG 
TGTCAATTTC AGATATCTGC ATATGAGGAT TCAGTGGCTA CTCATCTTGC AAAGATTCTA 
AATTCAGACA AGCATTCAGT AGTGATTTCC TCTGCTAAGG TTTTATGTGA AACTGTAAAG 
GACTTTGTTG CAAAAGTCGG GAAGGCTTAT GAGAAACCAA TGGAAAATGC AGAGGAAGCT 
GAATCAATGG CTGTAAAGTG TTCAACTCTG AATGAAAAAT TGGACCTGCT GCTTCAGACT 
CTGCATGCAG AATCTCAGGC TGCTCCATTG CTGGCACATT CCACCCCCCC TATTGTGGAA 
GAAGAAGTTG AGGAAGAGTT TTCCAGTGAA GAATCCCTGT CTGAAAGTAG AGAACAGTTA 
GCTGACAGCA TACCAAAGTC TTCCAGCCAA AGGCTCTTCA AACCAAGAGA GCAACTCATG 
CTGCGGGCTA ACAGCCTGAA AAAAGCTGTC AGGCAGATAA TAGAGCAAGC TGAGAAAGTG 
GTAGATGAGC AGAATGCACA CACTGAGGAA CAAGAAAACC AGTCTCCTGA GGAATACAGA 
AAGGAGTTGG AGGAATCTAA AGAGGAAGAA AAAGATGAAG ACACAAAAGA ATCTGAATTT 
CAGGAGTCTG GCAAGGTTCC CCCACGGTCA CCAGACAGAC AGACAAGCCG CAGTACATAT 
ACTCACACAG GCTCATTCTC AGGGCCACCC TTGGCATCAA GCAAGGAAAA CCTACCTGTG 
TTGAACACTA GAATAATCTG TCCAGGTCTG AGGGCAGGGT TGGCGGCCTC CATCGCTGGG 
AGTTCTATCA TCAATAAAAT GTTACTGGCA AACATAGATC CACTGGGGGC TACTCCATTT 
ATTGACCCTG ATCCAGATTC ACTGGAAGGC TATTCAGAAA AATGTGTAAT GAACAACTAT 
TTTGGAATTG GCCTGGATGC AAAGATATCC TTAGAATTTA ACAACAAGCG AGAAGAACAC 
CCTGAAAAAT GCAGAAGCCG AACTAAAAAT ATGATGTGGT ATGGTGTGCT TGGCACCAAG 
GAACTTCTGC AGCGAACATA CAAAAACTTA GAGCAGAAAG TCCAGCTTGA GTGTGATGGG 
CAGTACATCC CTCTTCCCAG CCTTCAGGGT ATTGCTGTGC TGAACATTCC AAGCTATGCT 
GGCGGCACTA ACTTCTGGGG TGGAACCAAG GAAGATGATA TCTTTGGTGC GCCGTCATTT 
GATGATAAAA TCTTGGAGGT GGTAGCAGTT TTTGGAAGTA TGCAGATGGC AGTATCTAGG 
GTGATAAAGC TGCAACATCA TAGAATAGCA CAGTGCCGGT CAGTGAAGAT CACCATACTT 
GGAGAAGAGG GAGTCCCTGT TCAGGTGGAT GGAGAGGCTT GGATTCAGCC ACCTGGCGTT 
ATTAAAATTG TACATAAAAA CCGAGCTCAG ATGCTGACTA GGGACAGGGC TTTTGAGAAC 
ACCCTGAAGT CCTGGGAAGA TAAGCAGAAG TACGATTCCT GTAAGCTTCC CCTTCGCTCT 
CATCTCTATT CTCAACAAGC TGTTGATTTG GCTTCAGAAG AAGAAGTCAC ACTAATCCAT 
CTTTGCTCGA AAGCTTCAGA GGAGCTTATT ACAAGAATAT GCGAAGCTGC AAAAATCCAT 
AGCTTGCTGG AACAAGAACT GGCTCATGCG GTGAATGCCT GCTCTCATGC ACTAAATAAA 
GCCAATCCCC GGTTTCCCGA GAGCCTAACA AGAAACACTG CCATTGAGGT AGCAGTAACT 
GCAAAAGCTT TGCACAATGA AACAGAATCT CTCTTGGTTG GAAGAGTGCC CCTGCAGTTG 
GAATCCCATC ATGAAGAACT TCTATCCAAT GCCCTTCACA GTGTAGAAGT TGAGCTAACA 
AAACTGGCTG AGATTCCTTG GCTGTACTTT GTCTTACAGC CAGATGAGGA GGATCCTCCT 
CTGGAATACT CTAAAAGAAA CAGCAGAAGC ACAGTATTCC GAATAGTGCC AAAGTTTAAA 
AGGGAGAAGG CCTTAAAGAA TAAGCCCAGC ATTCAGCCTG GATCTGGGGA TATTGAAAGT 
GGGTCATATG AAGCGAATTC TACAGGGAAT TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

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dgkh cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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