sptbn2 cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

Gene
Clones
gRNAs

Summary

Gene Symbol	sptbn2
Entrez Gene ID	100486199
Full Name	spectrin, beta, non-erythrocytic 2
General protein information	Preferred Names spectrin, beta, non-erythrocytic 2 Names spectrin beta chain, non-erythrocytic 2
Gene Type	protein-coding
Organism	Xenopus tropicalis(tropical clawed frog)

mRNA and Protein(s)

mRNA	Protein	Name
XM_004913695.3	XP_004913752.1	spectrin beta chain, non-erythrocytic 2 isoform X2
XM_004913695.4	XP_004913752.1	spectrin beta chain, non-erythrocytic 2 isoform X2
XM_002940505.5	XP_002940551.2	spectrin beta chain, non-erythrocytic 2 isoform X1
XM_012962405.2	XP_012817859.1	spectrin beta chain, non-erythrocytic 2 isoform X3
XM_012962405.3	XP_012817859.1	spectrin beta chain, non-erythrocytic 2 isoform X3
XM_002940505.4	XP_002940551.2	spectrin beta chain, non-erythrocytic 2 isoform X1

Pathways from BioSystems

Homologies

Homo sapiens (human)	SPTBN2	NP_008877.1
Macaca mulatta (Rhesus monkey)	SPTBN2	XP_001108262.2
Mus musculus (house mouse)	Sptbn2	NP_067262.1
Xenopus tropicalis (tropical clawed frog)	LOC100486199	XP_004913752.1
Pan troglodytes (chimpanzee)	SPTBN2	XP_001172486.2
Danio rerio (zebrafish)	LOC558044	NP_001093459.1
Rattus norvegicus (Norway rat)	Sptbn2	NP_062040.1
Canis lupus familiaris (dog)	SPTBN2	XP_540827.3

Gene Ontology

The following sptbn2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the sptbn2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OXa09015	XM_002940505.4 Latest version!	Xenopus tropicalis spectrin, beta, non-erythrocytic 2 (sptbn2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1301.30 $1859.00
OXa09016	XM_004913695.3 Latest version!	Xenopus tropicalis spectrin, beta, non-erythrocytic 2 (sptbn2), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1301.30 $1859.00
OXa33551	XM_012962405.2 Latest version!	Xenopus tropicalis spectrin, beta, non-erythrocytic 2 (sptbn2), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1301.30 $1859.00
OXa56729	XM_002940505.5 Latest version!	Xenopus tropicalis spectrin, beta, non-erythrocytic 2 (sptbn2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1301.30 $1859.00
OXa56730	XM_004913695.4 Latest version!	Xenopus tropicalis spectrin, beta, non-erythrocytic 2 (sptbn2), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1301.30 $1859.00
OXa56731	XM_012962405.3 Latest version!	Xenopus tropicalis spectrin, beta, non-erythrocytic 2 (sptbn2), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1301.30 $1859.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

sptbn2 ( XM_002940505.4 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_002940551.2 Xenopus tropicalis spectrin, beta, non-erythrocytic 2 (sptbn2), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OXa09015
Clone ID Related Accession (Same CDS sequence)	XM_002940505.4
Accession Version	XM_002940505.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7221bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1473177600000
Organism	Xenopus tropicalis(tropical clawed frog)
Product	spectrin beta chain, non-erythrocytic 2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030680.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_002940505.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTTCAGT TCGACTCCCC TGCATACGAG GATGTGCCCC GGCCTGCCTC CCCCGGTCCC 
CGGCTTGCCT CCCCTGTCCC CCGATCTGGC TCCCCTCTCC TATCCCCTGC GGCTTTTGTA 
AATGCAGTGC AATACGCTAA CGTGCTGGAA GGAAGGTTCA AGCAGCTGCA AGATGAAAGA 
GAAGCTGTGC AGAAGAAGAC ATTTACTAAA TGGGTCAACT CTCACTTGGC GAGGGTCACC 
TGTAGAATTT CTGATCTCTA CAATGATTTA CGAGATGGAC GGATGTTAAT TCGACTGTTA 
GAAGTGCTAT CTGGAGAGCA GCTGCCAAAA CCCACAAAAG GTCGAATGCG AATCCACTGT 
TTGGAAAATG TAGACAAAGC CCTTCAATTT TTAAAAGAGC AGAAGGTACA TTTGGAGAAC 
ATGGGATCAC ATGACATTGT GGATGGAAAC CATCGATTAA CCCTAGGGTT AATCTGGACT 
ATTATTTTAA GGTTTCAGAT CCAAGATATC AGTGTAGAGA CAGAAGATAA TAAAGAAAAG 
AAGTCTGCTA AAGATGCCTT ACTGCTTTGG TGCCAGATGA AGACTGCTGG GTATCCAAAT 
GTCAATGTTC ACAACTTTAC CACCAGCTGG CGAGATGGAC TTGCGTTTAA TGCCATTGTA 
CACAAGCACA GACCAGATAT AATTGACTTT GACACACTGA AGAAGTCTAA TGCACACCAC 
AACCTCCAGA ATGCCTTCAA TGTAGCTGAA AGAGAGTTAG GTCTTACTAA ACTTCTGGAT 
CCTGAAGATG TTAATGTGGA TCAGCCTGAT GAGAAATCCA TCATAACCTA TGTGGCCACA 
TTTTATCACT ATTTTTCCAA AATGAAAGCC CTAGCTGTGG AAGGAAAGCG TATTGGAAAG 
GTACTTGACC AAGCTATTGA AGCTGATCAG TTAATTGAGA ACTATGAAGG ACTGGCATCA 
GAACTCCTGC AATGGATTGA ACAGATGATC ATTACTCTCA ATGACCGAAA ACTGGCAAAC 
TCTCTAAGTG GTGTTCAGAA CCAGCTGCAG GCCTTCAACA CCTACAGGAC TGTAGAAAAG 
CCGCCCAAGT TCACAGAAAA GGGCAATTTG GAAGTTCTTC TCTTTACCAT ACAGAGTAAA 
ATGAGAGCTA ATAATCAAAA AGTGTATACG CCCAGAGAAG GAAAGCTTAT ATCTGATATA 
AACAAGGCAT GGGAGCGACT AGAAAAGGCA GAGCATGAAC GTGAGCTTGC CCTTCGCAAT 
GAACTTATAC GCCAGGAAAA ATTAGAGCAG TTGGCTGCAC GATTTGACCG TAAAGCAGCC 
ATGAGGGAAA CCTGGCTAAG TGAAAATCAG AGACTTGTAG CTCAGGATAA CTTCGGTGTG 
GACTTAACTG CGGTGGAGGC AGCGCTACGG AAACATGAAG CTATTGAAAC TGATATATTG 
GCTTACAGTG AGCGCGTGCA AGCAGTGAAT GCAGTGGCTG ATGAGCTGGA AGCAGAGAAT 
TACCATGATG TCCGGAGGAT TTTGGCTCGG AAGTGCAATG TGATTCGTCT CTCTGATTAT 
TTAAGAGAGC TTGTCACTGC CAGGAGGCAA AGGCTTCATA TGAACCTAGA ATTGCAGAGG 
ATGTTCCAGG ATTTACATTA TCTCATGGAC TGGACTGAAG ATATGAAGGG CCGTCTGCAG 
TCAGAAGATT ATGGCAAGCA TCTTCATGGC GTGGAAGACC TTCTCCAGAC TCACACTCTT 
GTGGAGGCAG ACATTGCAGT TCAGGCAGAA CGTGTGAAAT CAGTTACAGC TGTAGCTCAG 
AAATTTATGA CAGAAGATGA AGGCTACAAG CCTTGCGACC CAGAGCTAGT GCGCAACCGG 
GTGCATCTCT TGGAACAGCG CTATGCAGAG CTGTGTAATC TTTCTGCGGC TCGGAGATCA 
AAGCTGGAAG AATCCCGCCG TCTCTGGAAA TTTTTCTGGG ACATGGGAGA GGAGGAAGCC 
TGGATCCGTG AGCAGAGTAG ATTACTACTT TCTGATGACT ATGGGAAAGA CCTTACCAGC 
TCACTTAGGT TGATCAGCAA GCATAATGCC TTCAGAGGTG AGATGAGTGG ACACCGTGGG 
CCGTTGCAGC AGACTGTGGC TGAGGGTGAG GAACTAATAT CCAAAGGTCA CTTTGGTGCA 
GGAGAGATTG AGGAAAGGAT AAAAGAAGTG AAAGGCCAGT GGACAGAGAT GGAGGCCTTA 
GCTGAAGACA GGGAAAGGAG GCTAAGAGAG GCCTACAGCT ATTATCAGTT TCAAGCTGAT 
GCCAATGATG CTGAGGCTTG GATCCTGGAC ACTTTACGCC TTGTTTCTAC CAGTGAAGTA 
GGACATGATG AGTATTCCAC ACAGACACTG GTGAAAAAGC ACAAAGAAAT AGAAGAGGAG 
ATCCTTAGCC ACCGACCAGC TATTGATGCG CTGCATGAAC AGGCCCGTGG CCTTCCACCT 
GCTTACTCTC ATTCACCTGA AGTTGATGGA AGGTTACCAG CCCTGGAGCA GCGCTATGAG 
GAATTGGTGG CTCTTGCTGC TCACCGCAAG CAGGCTCTGC AAGATTCTCT GGCTCTTCAT 
AAAATGTTTA GTGAAGCTGA TGCTTGCCTT TTGTGGATTG ATGAGAAAGA ACAGTGGCTT 
AATGTCACAG AGATTCCAGA AAAACTTGAG GACCTAGAAG TAGTTCAACA AAGGTTTGAA 
ACTCTAGAGG CAGAGATGAA TAATTTGGCA TCCCGGATTG CATCTGTAAA TGAAGTTGCT 
GGACAGTTGC TGACTACAGA CCAGAGAAAT GAAAACAGCA TCCGAGACAT GCAAGATCAA 
CTTAATTCTC GGTGGAATGA ATTCCGCAAG CTTGTTGACC AAAAGAAGGG TGCTTTGGAT 
TCTGCTCTGG ATATACAGAA TTATCATTTG GAGTGCAATG AGACTGTGTG CTGGATGAGG 
GAGAAGACAA AGGTTATTGA ATCCACACAG AGCCTAGGGA ATGATCTTGC AGGAGTAACA 
GCCCTCCAGA GAAAACTTAC TGGAATGGAG CGTGATCTGA AAGCCATTCA GGGAAAAGTG 
GGTGACCTGC GTTCAGAGGC AGAAAAGCTT GCAGATGGGC ACCCACAGAA AGCACCGGCC 
ATAATTGGCC GCTTATCAGA TATTGATGAG ACCTGGGGAG AACTAAAGGA CACTATGAAA 
AGAAGAGAAG AAACTTTAGG TGAAGCAAGC AAGCTGCAGG GATTTTTGAG AGACCTTGAT 
GATTTCCAGA GCTGGTTGTC CCGGGCCCAG ACAGCAGTGG CATCTGAGGA TGTTCCAGCT 
GACCTAGCAG AAGCAGAGCG CCTTCTTGCA CAACATGAAG GCATAAAAAA TGAGATTGAT 
CATTATGGAG CTGACTACCA CAGAGTGAGA GATGTTGGTG AAGAAGTCAC TCGTGGGCAA 
ACAGATGCCC AATATATGTT CTTGCAACAG AGACTGAAGG CGCTAGACAC TGGCTGGAAT 
GAGCTTGGAC AGATGTGGGA AAACAGGCAC CAGTTGTTGT CCCAGGGTCA CAGCTTCCAG 
CAGTTTTTGA AAGATACAAA ACAAGTGGAG GGGTTCCTTA ATAACCAGGA GTATGTCCTT 
TCCCATACCG AAATGCCAAG TACGCTACAG GCAGCTGAAG CTGCCATCAA ACGCCATGAA 
GATTTCCTCA CAACCATGGA TGCCAATGAA GAGAAGATAC AGGGTCTTGT AGAATCTGGG 
AGGAAGCTAA TTGAGGAACA GAATGTCAAT TCTGAGAAGA TTCAAGAAAA AGTCACTTCT 
ATTGATGAGA GACACAAAAA GAACCAAAAA ACTGCTAGCG AAGTATTAAC TCGTTTGAAG 
GACAACCGTG ATCTTCAACA TTTCCTTCAA GACTGTCAAG AGCTCACCCT CTGGATCAAT 
GAAAAAATGC TTACTGCTCA AGATGTTTCT TATGATGAAG CTCGAAACCT GCATAGCAAG 
TGGCAAAAGC ATCAGGCATT CATGGCAGAA CTGGCCTCAA ATAAAGACTG GCTTGACAAA 
ATAGACAAGG AAGGGCAGCA GCTCATGTCA GAAAAACCGG AGCTTGAACC AGTAGTGAGA 
GAAAAACTAG ACACTCTGCA TAAGCTGTGG AATGACTTGG AATCTACCAC ACAAAGTAAA 
GCTCAGTGTC TGTTTGATGC CAACAGGGCA GAGTTATTCA CACAAAGCTG TTCTGCTCTG 
GAGAGTTGGC TTCAAAACCT CGAGTCACAA CTACAGTCAG ATCATTATGG CAAAGATCTG 
ACCAGTGTCA ACATCCTATT AAATAAACAG CAGATGCTGG AGAACCAAAT GGAAGTGAGG 
GAGAAGGAGC TAGAAGCTAT ACAGTCCCAG GCACAGGCCC TTGGACAGGA GGATGCCAAC 
ATAGTGGAGG TTGACTCAAA GCATAAGGAT GTAGCAGATA AATTTTCCAA GCTGCGTGCT 
CCTCTAAACA AGAGAAGAAA ATTACTTCTA GCCTCCAAGG AAGGACATCA GTTTAACAGG 
GACCTGGAAG ATGAAATACT TTGGGTAAAG GAGAAGATGC CAATGGCAAT CTCCACTGAT 
CATGGAAAAG ATCTCCCGAC AGTCCAGCTT CTTATCAAAA AGAATCAGAC ACTCCAAAAG 
GAAATTTTGG GACATCAACC ACGGATTGAT GACATCCTGG AGAGAGGCAA ATCTATGATC 
CAAGACAGCT CTCTGGACCC TCAAGAGATA GAAGAAAGGC TTGGGCTACT GAAAAGCACA 
TGGGAAGAGC TGGCAGCAGA AGGAGGAAAG CGCCATGCCC GTTTAGAGGA ATCTCTTGCT 
GCTCAGCAGT TTTATGCTGA TGCGGCAGAA GCTGAGGCCT GGATGGGAGA GCAAGAGTTA 
CATATGATGT CTGAAGAAAA AGCCAAGGAT GAGATTGGTG CCCAGGCTAT GCTGAAGAAA 
CACTTAGTAA TGGAACAGGC TCTTGATGAC TATGCAGACA ACATCCATCA GCTTGCCAAT 
TCAAGCCGCG ACATGGTCTC CAGAGGTCAT CCAGAAAGTG ATCGTATTAC CCTTCGTCAA 
GGGCAAGTGG ACAAGCTATA TGCCAGTCTT AAAGACCTGG CAGAGGAGAG ACAAGGAAAG 
CTACAAGAGC ACCACAGACT TTGCCAACTC AAGAGAGATG TCGAAGACCT TGAGCACTGG 
ATCTCTGAAC GTGAGGTGGT TGCAGCTTCT CATGAACTTG GGCAAGATTA TGAGCATGTA 
ACGATGCTGA GAGACAAATT CCGTGAGTTC TCCCGTGACA CCAGTAACAT TGGTCAAGAG 
CGGGTGGATG CTGTCAATCT GATGGCAGAT GATTTAATTT CATCTGGACA TTCGGAAAAT 
GCTACTGTAG CAGATTGGAA AGACCAGCTA AATGAGGCAT GGGCTGATCT TCTTGAGATG 
ATCGACACTC GAAGTCAGAT GCTGGCAGCC TCATATGAAC TTCACCGTTT CTACCAGGAT 
GCAAAAGAAA CCCTAGGTCA GATCCAAGAC AAGCAGAAAC AAATACCAGA GGAGACAGGC 
AGAGATCTGA ATACAGCAGA AGCCATGCAG CGCATGCACA CCGCTTTTGA ACATGATATC 
CAAGCCCTGA GTGCACAGGT GAAGCAAGTC CAGGAAGATG CTTCTCGCCT CCAAAAAGCT 
TATGCTGGTG AAAAGGCAGC AGACATTCAT CGCCATGAGC AGGCAGTATC GGAAGCTTGG 
GGAGAGTTGC AGAATGCTAG TGGTGCCAGG CGGCAGCTTC TTCTTGACAC TGTGGAGAAA 
TTCCGTTTCT TCAGAATGGT GCGAGATCTC ATGTTATGGA TGGATGATAT AAACCTGCTA 
ATTGATGCAC AGGAGAAGCC AAGAGATGTA TCCTCAGCTG ATTTAGTCAT CCAAAACCAC 
CTAGGCATCA AGGCAGAAGT GGATGCGCGA GCTGACAGCT TTACTACATG CATAGATCTT 
GGCAACTCTC TCCTAGAGAA GAATCATTAT GCCTCTGACA AGATTTCAGA GAAACTGGAG 
CAATTGCAGG AAAGAAGGCA GGAGACTGAA AACAAATGGA AAGATAAAAT GGCATGGCTG 
AGAGTTGTCC ACGAAGTGCT GATGTTTGGA AGAGATGCAA GTGTAGCTGA GGCTTGGCTG 
GCCAGCCAGG AGCCCATTGT CCGGTCGTCA GAACTTGGTG GCAATGTCGA TGAAGTAGAA 
TCCCTGATAA AAAGACACGA AGCCTTTCAG AAAGCAGTTT CTACATGGGA TGAGAGATTT 
GTTCTTCTTG AAAAACTAAC CACGTTAGAA GAAATTGAAA TGCGTCGGAA GCGGGAAGAG 
GAAGAAAGAA AGAAAAGACC ACCAACACCA CCTCTACCAG TAGAAGAGCC TCGAGAGGAA 
GTTGTAAAAA CCCAAGTTTC CCAGACTATT GAAGGACAGA CTGATCCAGC CAATATACTT 
CAAGAAAGGA TAAATGGCAT GCAGAATTCA TTCCAGGAGC AAGTATCTGT TAATGGACTG 
TGCACAGAGT CTGATTCTCC ACAGCCATCG TCCGATACAC AGAGGTTAGA AGACAGCATC 
TCTGAAAATC AGGGTCCGGA ACAGTCAGAT GTAACCAATG GAGCCAGGAC TGAGGTTATT 
ACCAAAGATG GAAGTCCAAC ACCTTCTCCA CAACTTGTGG CTAGGCCAAC CTTCTCCCCG 
ATAAACCTCC AGTATTCTAC TTTGCCATCT AGCCCCACTG AACCATTGGC ATCTGAGCAA 
ATGGAAGGAA TGCTGCACCG CAAGCAGGAA ATGGATTCTC ACAACAAGAA GGCTGCGAAC 
AGGTCCTGGC AGAATGTTTA CTGTGCACTG AAAAGGGGCA GTCTGGGTTT CTACAAGGAT 
GCTAAAAGTG CAGCCAGTGG CGTTTCTTAT CATTCGGAGC CCTCCGTCCA ACTTAATGGA 
GCCCAGTGCA GTGTTGCTCA TGACTACAAG AAACGCAAGA ATGTCTTCAA GCTAAGCCTT 
CTTGATGGCA AAGAATATCT GTTCCAAGCT AAGGATGAGG CTGAAATGAG TAGTTGGATG 
CGAGTTATAA ACGCCTCCCT AACTTCCTCC CCGGGAGACC CCTCAGACCA TTCACCGGGC 
ATCATGAAGG GTATGACCCG GGCCATGACA ATGCCACCAG TATCACCCAA CAGTGGTACA 
GAGGGATCAG TCACATTGCG CAACAAGGAC GGGAAAGAAA AAGACCGGGA AAAGAGATTC 
AGTTTCTTTA AGAAGAAGTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_002940551.2
CDS	908..8128
Translation	MFQFDSPAYEDVPRPASPGPRLASPVPRSGSPLLSPAAFVNAVQYANVLEGRFKQLQDEREAVQKKTFTKWVNSHLARVTCRISDLYNDLRDGRMLIRLLEVLSGEQLPKPTKGRMRIHCLENVDKALQFLKEQKVHLENMGSHDIVDGNHRLTLGLIWTIILRFQIQDISVETEDNKEKKSAKDALLLWCQMKTAGYPNVNVHNFTTSWRDGLAFNAIVHKHRPDIIDFDTLKKSNAHHNLQNAFNVAERELGLTKLLDPEDVNVDQPDEKSIITYVATFYHYFSKMKALAVEGKRIGKVLDQAIEADQLIENYEGLASELLQWIEQMIITLNDRKLANSLSGVQNQLQAFNTYRTVEKPPKFTEKGNLEVLLFTIQSKMRANNQKVYTPREGKLISDINKAWERLEKAEHERELALRNELIRQEKLEQLAARFDRKAAMRETWLSENQRLVAQDNFGVDLTAVEAALRKHEAIETDILAYSERVQAVNAVADELEAENYHDVRRILARKCNVIRLSDYLRELVTARRQRLHMNLELQRMFQDLHYLMDWTEDMKGRLQSEDYGKHLHGVEDLLQTHTLVEADIAVQAERVKSVTAVAQKFMTEDEGYKPCDPELVRNRVHLLEQRYAELCNLSAARRSKLEESRRLWKFFWDMGEEEAWIREQSRLLLSDDYGKDLTSSLRLISKHNAFRGEMSGHRGPLQQTVAEGEELISKGHFGAGEIEERIKEVKGQWTEMEALAEDRERRLREAYSYYQFQADANDAEAWILDTLRLVSTSEVGHDEYSTQTLVKKHKEIEEEILSHRPAIDALHEQARGLPPAYSHSPEVDGRLPALEQRYEELVALAAHRKQALQDSLALHKMFSEADACLLWIDEKEQWLNVTEIPEKLEDLEVVQQRFETLEAEMNNLASRIASVNEVAGQLLTTDQRNENSIRDMQDQLNSRWNEFRKLVDQKKGALDSALDIQNYHLECNETVCWMREKTKVIESTQSLGNDLAGVTALQRKLTGMERDLKAIQGKVGDLRSEAEKLADGHPQKAPAIIGRLSDIDETWGELKDTMKRREETLGEASKLQGFLRDLDDFQSWLSRAQTAVASEDVPADLAEAERLLAQHEGIKNEIDHYGADYHRVRDVGEEVTRGQTDAQYMFLQQRLKALDTGWNELGQMWENRHQLLSQGHSFQQFLKDTKQVEGFLNNQEYVLSHTEMPSTLQAAEAAIKRHEDFLTTMDANEEKIQGLVESGRKLIEEQNVNSEKIQEKVTSIDERHKKNQKTASEVLTRLKDNRDLQHFLQDCQELTLWINEKMLTAQDVSYDEARNLHSKWQKHQAFMAELASNKDWLDKIDKEGQQLMSEKPELEPVVREKLDTLHKLWNDLESTTQSKAQCLFDANRAELFTQSCSALESWLQNLESQLQSDHYGKDLTSVNILLNKQQMLENQMEVREKELEAIQSQAQALGQEDANIVEVDSKHKDVADKFSKLRAPLNKRRKLLLASKEGHQFNRDLEDEILWVKEKMPMAISTDHGKDLPTVQLLIKKNQTLQKEILGHQPRIDDILERGKSMIQDSSLDPQEIEERLGLLKSTWEELAAEGGKRHARLEESLAAQQFYADAAEAEAWMGEQELHMMSEEKAKDEIGAQAMLKKHLVMEQALDDYADNIHQLANSSRDMVSRGHPESDRITLRQGQVDKLYASLKDLAEERQGKLQEHHRLCQLKRDVEDLEHWISEREVVAASHELGQDYEHVTMLRDKFREFSRDTSNIGQERVDAVNLMADDLISSGHSENATVADWKDQLNEAWADLLEMIDTRSQMLAASYELHRFYQDAKETLGQIQDKQKQIPEETGRDLNTAEAMQRMHTAFEHDIQALSAQVKQVQEDASRLQKAYAGEKAADIHRHEQAVSEAWGELQNASGARRQLLLDTVEKFRFFRMVRDLMLWMDDINLLIDAQEKPRDVSSADLVIQNHLGIKAEVDARADSFTTCIDLGNSLLEKNHYASDKISEKLEQLQERRQETENKWKDKMAWLRVVHEVLMFGRDASVAEAWLASQEPIVRSSELGGNVDEVESLIKRHEAFQKAVSTWDERFVLLEKLTTLEEIEMRRKREEEERKKRPPTPPLPVEEPREEVVKTQVSQTIEGQTDPANILQERINGMQNSFQEQVSVNGLCTESDSPQPSSDTQRLEDSISENQGPEQSDVTNGARTEVITKDGSPTPSPQLVARPTFSPINLQYSTLPSSPTEPLASEQMEGMLHRKQEMDSHNKKAANRSWQNVYCALKRGSLGFYKDAKSAASGVSYHSEPSVQLNGAQCSVAHDYKKRKNVFKLSLLDGKEYLFQAKDEAEMSSWMRVINASLTSSPGDPSDHSPGIMKGMTRAMTMPPVSPNSGTEGSVTLRNKDGKEKDREKRFSFFKKK

Target ORF information:

RefSeq Version	XM_002940505.4
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis spectrin, beta, non-erythrocytic 2 (sptbn2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_002940505.4

ORF Insert Sequence:

ATGTTTCAGT TCGACTCCCC TGCATACGAG GATGTGCCCC GGCCTGCCTC CCCCGGTCCC 
CGGCTTGCCT CCCCTGTCCC CCGATCTGGC TCCCCTCTCC TATCCCCTGC GGCTTTTGTA 
AATGCAGTGC AATACGCTAA CGTGCTGGAA GGAAGGTTCA AGCAGCTGCA AGATGAAAGA 
GAAGCTGTGC AGAAGAAGAC ATTTACTAAA TGGGTCAACT CTCACTTGGC GAGGGTCACC 
TGTAGAATTT CTGATCTCTA CAATGATTTA CGAGATGGAC GGATGTTAAT TCGACTGTTA 
GAAGTGCTAT CTGGAGAGCA GCTGCCAAAA CCCACAAAAG GTCGAATGCG AATCCACTGT 
TTGGAAAATG TAGACAAAGC CCTTCAATTT TTAAAAGAGC AGAAGGTACA TTTGGAGAAC 
ATGGGATCAC ATGACATTGT GGATGGAAAC CATCGATTAA CCCTAGGGTT AATCTGGACT 
ATTATTTTAA GGTTTCAGAT CCAAGATATC AGTGTAGAGA CAGAAGATAA TAAAGAAAAG 
AAGTCTGCTA AAGATGCCTT ACTGCTTTGG TGCCAGATGA AGACTGCTGG GTATCCAAAT 
GTCAATGTTC ACAACTTTAC CACCAGCTGG CGAGATGGAC TTGCGTTTAA TGCCATTGTA 
CACAAGCACA GACCAGATAT AATTGACTTT GACACACTGA AGAAGTCTAA TGCACACCAC 
AACCTCCAGA ATGCCTTCAA TGTAGCTGAA AGAGAGTTAG GTCTTACTAA ACTTCTGGAT 
CCTGAAGATG TTAATGTGGA TCAGCCTGAT GAGAAATCCA TCATAACCTA TGTGGCCACA 
TTTTATCACT ATTTTTCCAA AATGAAAGCC CTAGCTGTGG AAGGAAAGCG TATTGGAAAG 
GTACTTGACC AAGCTATTGA AGCTGATCAG TTAATTGAGA ACTATGAAGG ACTGGCATCA 
GAACTCCTGC AATGGATTGA ACAGATGATC ATTACTCTCA ATGACCGAAA ACTGGCAAAC 
TCTCTAAGTG GTGTTCAGAA CCAGCTGCAG GCCTTCAACA CCTACAGGAC TGTAGAAAAG 
CCGCCCAAGT TCACAGAAAA GGGCAATTTG GAAGTTCTTC TCTTTACCAT ACAGAGTAAA 
ATGAGAGCTA ATAATCAAAA AGTGTATACG CCCAGAGAAG GAAAGCTTAT ATCTGATATA 
AACAAGGCAT GGGAGCGACT AGAAAAGGCA GAGCATGAAC GTGAGCTTGC CCTTCGCAAT 
GAACTTATAC GCCAGGAAAA ATTAGAGCAG TTGGCTGCAC GATTTGACCG TAAAGCAGCC 
ATGAGGGAAA CCTGGCTAAG TGAAAATCAG AGACTTGTAG CTCAGGATAA CTTCGGTGTG 
GACTTAACTG CGGTGGAGGC AGCGCTACGG AAACATGAAG CTATTGAAAC TGATATATTG 
GCTTACAGTG AGCGCGTGCA AGCAGTGAAT GCAGTGGCTG ATGAGCTGGA AGCAGAGAAT 
TACCATGATG TCCGGAGGAT TTTGGCTCGG AAGTGCAATG TGATTCGTCT CTCTGATTAT 
TTAAGAGAGC TTGTCACTGC CAGGAGGCAA AGGCTTCATA TGAACCTAGA ATTGCAGAGG 
ATGTTCCAGG ATTTACATTA TCTCATGGAC TGGACTGAAG ATATGAAGGG CCGTCTGCAG 
TCAGAAGATT ATGGCAAGCA TCTTCATGGC GTGGAAGACC TTCTCCAGAC TCACACTCTT 
GTGGAGGCAG ACATTGCAGT TCAGGCAGAA CGTGTGAAAT CAGTTACAGC TGTAGCTCAG 
AAATTTATGA CAGAAGATGA AGGCTACAAG CCTTGCGACC CAGAGCTAGT GCGCAACCGG 
GTGCATCTCT TGGAACAGCG CTATGCAGAG CTGTGTAATC TTTCTGCGGC TCGGAGATCA 
AAGCTGGAAG AATCCCGCCG TCTCTGGAAA TTTTTCTGGG ACATGGGAGA GGAGGAAGCC 
TGGATCCGTG AGCAGAGTAG ATTACTACTT TCTGATGACT ATGGGAAAGA CCTTACCAGC 
TCACTTAGGT TGATCAGCAA GCATAATGCC TTCAGAGGTG AGATGAGTGG ACACCGTGGG 
CCGTTGCAGC AGACTGTGGC TGAGGGTGAG GAACTAATAT CCAAAGGTCA CTTTGGTGCA 
GGAGAGATTG AGGAAAGGAT AAAAGAAGTG AAAGGCCAGT GGACAGAGAT GGAGGCCTTA 
GCTGAAGACA GGGAAAGGAG GCTAAGAGAG GCCTACAGCT ATTATCAGTT TCAAGCTGAT 
GCCAATGATG CTGAGGCTTG GATCCTGGAC ACTTTACGCC TTGTTTCTAC CAGTGAAGTA 
GGACATGATG AGTATTCCAC ACAGACACTG GTGAAAAAGC ACAAAGAAAT AGAAGAGGAG 
ATCCTTAGCC ACCGACCAGC TATTGATGCG CTGCATGAAC AGGCCCGTGG CCTTCCACCT 
GCTTACTCTC ATTCACCTGA AGTTGATGGA AGGTTACCAG CCCTGGAGCA GCGCTATGAG 
GAATTGGTGG CTCTTGCTGC TCACCGCAAG CAGGCTCTGC AAGATTCTCT GGCTCTTCAT 
AAAATGTTTA GTGAAGCTGA TGCTTGCCTT TTGTGGATTG ATGAGAAAGA ACAGTGGCTT 
AATGTCACAG AGATTCCAGA AAAACTTGAG GACCTAGAAG TAGTTCAACA AAGGTTTGAA 
ACTCTAGAGG CAGAGATGAA TAATTTGGCA TCCCGGATTG CATCTGTAAA TGAAGTTGCT 
GGACAGTTGC TGACTACAGA CCAGAGAAAT GAAAACAGCA TCCGAGACAT GCAAGATCAA 
CTTAATTCTC GGTGGAATGA ATTCCGCAAG CTTGTTGACC AAAAGAAGGG TGCTTTGGAT 
TCTGCTCTGG ATATACAGAA TTATCATTTG GAGTGCAATG AGACTGTGTG CTGGATGAGG 
GAGAAGACAA AGGTTATTGA ATCCACACAG AGCCTAGGGA ATGATCTTGC AGGAGTAACA 
GCCCTCCAGA GAAAACTTAC TGGAATGGAG CGTGATCTGA AAGCCATTCA GGGAAAAGTG 
GGTGACCTGC GTTCAGAGGC AGAAAAGCTT GCAGATGGGC ACCCACAGAA AGCACCGGCC 
ATAATTGGCC GCTTATCAGA TATTGATGAG ACCTGGGGAG AACTAAAGGA CACTATGAAA 
AGAAGAGAAG AAACTTTAGG TGAAGCAAGC AAGCTGCAGG GATTTTTGAG AGACCTTGAT 
GATTTCCAGA GCTGGTTGTC CCGGGCCCAG ACAGCAGTGG CATCTGAGGA TGTTCCAGCT 
GACCTAGCAG AAGCAGAGCG CCTTCTTGCA CAACATGAAG GCATAAAAAA TGAGATTGAT 
CATTATGGAG CTGACTACCA CAGAGTGAGA GATGTTGGTG AAGAAGTCAC TCGTGGGCAA 
ACAGATGCCC AATATATGTT CTTGCAACAG AGACTGAAGG CGCTAGACAC TGGCTGGAAT 
GAGCTTGGAC AGATGTGGGA AAACAGGCAC CAGTTGTTGT CCCAGGGTCA CAGCTTCCAG 
CAGTTTTTGA AAGATACAAA ACAAGTGGAG GGGTTCCTTA ATAACCAGGA GTATGTCCTT 
TCCCATACCG AAATGCCAAG TACGCTACAG GCAGCTGAAG CTGCCATCAA ACGCCATGAA 
GATTTCCTCA CAACCATGGA TGCCAATGAA GAGAAGATAC AGGGTCTTGT AGAATCTGGG 
AGGAAGCTAA TTGAGGAACA GAATGTCAAT TCTGAGAAGA TTCAAGAAAA AGTCACTTCT 
ATTGATGAGA GACACAAAAA GAACCAAAAA ACTGCTAGCG AAGTATTAAC TCGTTTGAAG 
GACAACCGTG ATCTTCAACA TTTCCTTCAA GACTGTCAAG AGCTCACCCT CTGGATCAAT 
GAAAAAATGC TTACTGCTCA AGATGTTTCT TATGATGAAG CTCGAAACCT GCATAGCAAG 
TGGCAAAAGC ATCAGGCATT CATGGCAGAA CTGGCCTCAA ATAAAGACTG GCTTGACAAA 
ATAGACAAGG AAGGGCAGCA GCTCATGTCA GAAAAACCGG AGCTTGAACC AGTAGTGAGA 
GAAAAACTAG ACACTCTGCA TAAGCTGTGG AATGACTTGG AATCTACCAC ACAAAGTAAA 
GCTCAGTGTC TGTTTGATGC CAACAGGGCA GAGTTATTCA CACAAAGCTG TTCTGCTCTG 
GAGAGTTGGC TTCAAAACCT CGAGTCACAA CTACAGTCAG ATCATTATGG CAAAGATCTG 
ACCAGTGTCA ACATCCTATT AAATAAACAG CAGATGCTGG AGAACCAAAT GGAAGTGAGG 
GAGAAGGAGC TAGAAGCTAT ACAGTCCCAG GCACAGGCCC TTGGACAGGA GGATGCCAAC 
ATAGTGGAGG TTGACTCAAA GCATAAGGAT GTAGCAGATA AATTTTCCAA GCTGCGTGCT 
CCTCTAAACA AGAGAAGAAA ATTACTTCTA GCCTCCAAGG AAGGACATCA GTTTAACAGG 
GACCTGGAAG ATGAAATACT TTGGGTAAAG GAGAAGATGC CAATGGCAAT CTCCACTGAT 
CATGGAAAAG ATCTCCCGAC AGTCCAGCTT CTTATCAAAA AGAATCAGAC ACTCCAAAAG 
GAAATTTTGG GACATCAACC ACGGATTGAT GACATCCTGG AGAGAGGCAA ATCTATGATC 
CAAGACAGCT CTCTGGACCC TCAAGAGATA GAAGAAAGGC TTGGGCTACT GAAAAGCACA 
TGGGAAGAGC TGGCAGCAGA AGGAGGAAAG CGCCATGCCC GTTTAGAGGA ATCTCTTGCT 
GCTCAGCAGT TTTATGCTGA TGCGGCAGAA GCTGAGGCCT GGATGGGAGA GCAAGAGTTA 
CATATGATGT CTGAAGAAAA AGCCAAGGAT GAGATTGGTG CCCAGGCTAT GCTGAAGAAA 
CACTTAGTAA TGGAACAGGC TCTTGATGAC TATGCAGACA ACATCCATCA GCTTGCCAAT 
TCAAGCCGCG ACATGGTCTC CAGAGGTCAT CCAGAAAGTG ATCGTATTAC CCTTCGTCAA 
GGGCAAGTGG ACAAGCTATA TGCCAGTCTT AAAGACCTGG CAGAGGAGAG ACAAGGAAAG 
CTACAAGAGC ACCACAGACT TTGCCAACTC AAGAGAGATG TCGAAGACCT TGAGCACTGG 
ATCTCTGAAC GTGAGGTGGT TGCAGCTTCT CATGAACTTG GGCAAGATTA TGAGCATGTA 
ACGATGCTGA GAGACAAATT CCGTGAGTTC TCCCGTGACA CCAGTAACAT TGGTCAAGAG 
CGGGTGGATG CTGTCAATCT GATGGCAGAT GATTTAATTT CATCTGGACA TTCGGAAAAT 
GCTACTGTAG CAGATTGGAA AGACCAGCTA AATGAGGCAT GGGCTGATCT TCTTGAGATG 
ATCGACACTC GAAGTCAGAT GCTGGCAGCC TCATATGAAC TTCACCGTTT CTACCAGGAT 
GCAAAAGAAA CCCTAGGTCA GATCCAAGAC AAGCAGAAAC AAATACCAGA GGAGACAGGC 
AGAGATCTGA ATACAGCAGA AGCCATGCAG CGCATGCACA CCGCTTTTGA ACATGATATC 
CAAGCCCTGA GTGCACAGGT GAAGCAAGTC CAGGAAGATG CTTCTCGCCT CCAAAAAGCT 
TATGCTGGTG AAAAGGCAGC AGACATTCAT CGCCATGAGC AGGCAGTATC GGAAGCTTGG 
GGAGAGTTGC AGAATGCTAG TGGTGCCAGG CGGCAGCTTC TTCTTGACAC TGTGGAGAAA 
TTCCGTTTCT TCAGAATGGT GCGAGATCTC ATGTTATGGA TGGATGATAT AAACCTGCTA 
ATTGATGCAC AGGAGAAGCC AAGAGATGTA TCCTCAGCTG ATTTAGTCAT CCAAAACCAC 
CTAGGCATCA AGGCAGAAGT GGATGCGCGA GCTGACAGCT TTACTACATG CATAGATCTT 
GGCAACTCTC TCCTAGAGAA GAATCATTAT GCCTCTGACA AGATTTCAGA GAAACTGGAG 
CAATTGCAGG AAAGAAGGCA GGAGACTGAA AACAAATGGA AAGATAAAAT GGCATGGCTG 
AGAGTTGTCC ACGAAGTGCT GATGTTTGGA AGAGATGCAA GTGTAGCTGA GGCTTGGCTG 
GCCAGCCAGG AGCCCATTGT CCGGTCGTCA GAACTTGGTG GCAATGTCGA TGAAGTAGAA 
TCCCTGATAA AAAGACACGA AGCCTTTCAG AAAGCAGTTT CTACATGGGA TGAGAGATTT 
GTTCTTCTTG AAAAACTAAC CACGTTAGAA GAAATTGAAA TGCGTCGGAA GCGGGAAGAG 
GAAGAAAGAA AGAAAAGACC ACCAACACCA CCTCTACCAG TAGAAGAGCC TCGAGAGGAA 
GTTGTAAAAA CCCAAGTTTC CCAGACTATT GAAGGACAGA CTGATCCAGC CAATATACTT 
CAAGAAAGGA TAAATGGCAT GCAGAATTCA TTCCAGGAGC AAGTATCTGT TAATGGACTG 
TGCACAGAGT CTGATTCTCC ACAGCCATCG TCCGATACAC AGAGGTTAGA AGACAGCATC 
TCTGAAAATC AGGGTCCGGA ACAGTCAGAT GTAACCAATG GAGCCAGGAC TGAGGTTATT 
ACCAAAGATG GAAGTCCAAC ACCTTCTCCA CAACTTGTGG CTAGGCCAAC CTTCTCCCCG 
ATAAACCTCC AGTATTCTAC TTTGCCATCT AGCCCCACTG AACCATTGGC ATCTGAGCAA 
ATGGAAGGAA TGCTGCACCG CAAGCAGGAA ATGGATTCTC ACAACAAGAA GGCTGCGAAC 
AGGTCCTGGC AGAATGTTTA CTGTGCACTG AAAAGGGGCA GTCTGGGTTT CTACAAGGAT 
GCTAAAAGTG CAGCCAGTGG CGTTTCTTAT CATTCGGAGC CCTCCGTCCA ACTTAATGGA 
GCCCAGTGCA GTGTTGCTCA TGACTACAAG AAACGCAAGA ATGTCTTCAA GCTAAGCCTT 
CTTGATGGCA AAGAATATCT GTTCCAAGCT AAGGATGAGG CTGAAATGAG TAGTTGGATG 
CGAGTTATAA ACGCCTCCCT AACTTCCTCC CCGGGAGACC CCTCAGACCA TTCACCGGGC 
ATCATGAAGG GTATGACCCG GGCCATGACA ATGCCACCAG TATCACCCAA CAGTGGTACA 
GAGGGATCAG TCACATTGCG CAACAAGGAC GGGAAAGAAA AAGACCGGGA AAAGAGATTC 
AGTTTCTTTA AGAAGAAGTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

sptbn2 ( XM_004913695.3 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_004913752.1 Xenopus tropicalis spectrin, beta, non-erythrocytic 2 (sptbn2), transcript variant X2, mRNA.

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CloneID	OXa09016
Clone ID Related Accession (Same CDS sequence)	XM_004913695.3
Accession Version	XM_004913695.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7206bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1473177600000
Organism	Xenopus tropicalis(tropical clawed frog)
Product	spectrin beta chain, non-erythrocytic 2 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030680.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_004913695.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCAGTC CACTGTCACC GACAGATTAT GACAGCCTGG AAATCCAGCA GCAGTATAAT 
GACATCAACA ATCGATGGGA TCTGACAGAT GATGATTGGG ACAATGAGAA CAGTTCTGCA 
AGACTTTTTG AGAGGTCCCG GATCAAAGCA CTTGCAGATG AAAGAGAAGC TGTGCAGAAG 
AAGACATTTA CTAAATGGGT CAACTCTCAC TTGGCGAGGG TCACCTGTAG AATTTCTGAT 
CTCTACAATG ATTTACGAGA TGGACGGATG TTAATTCGAC TGTTAGAAGT GCTATCTGGA 
GAGCAGCTGC CAAAACCCAC AAAAGGTCGA ATGCGAATCC ACTGTTTGGA AAATGTAGAC 
AAAGCCCTTC AATTTTTAAA AGAGCAGAAG GTACATTTGG AGAACATGGG ATCACATGAC 
ATTGTGGATG GAAACCATCG ATTAACCCTA GGGTTAATCT GGACTATTAT TTTAAGGTTT 
CAGATCCAAG ATATCAGTGT AGAGACAGAA GATAATAAAG AAAAGAAGTC TGCTAAAGAT 
GCCTTACTGC TTTGGTGCCA GATGAAGACT GCTGGGTATC CAAATGTCAA TGTTCACAAC 
TTTACCACCA GCTGGCGAGA TGGACTTGCG TTTAATGCCA TTGTACACAA GCACAGACCA 
GATATAATTG ACTTTGACAC ACTGAAGAAG TCTAATGCAC ACCACAACCT CCAGAATGCC 
TTCAATGTAG CTGAAAGAGA GTTAGGTCTT ACTAAACTTC TGGATCCTGA AGATGTTAAT 
GTGGATCAGC CTGATGAGAA ATCCATCATA ACCTATGTGG CCACATTTTA TCACTATTTT 
TCCAAAATGA AAGCCCTAGC TGTGGAAGGA AAGCGTATTG GAAAGGTACT TGACCAAGCT 
ATTGAAGCTG ATCAGTTAAT TGAGAACTAT GAAGGACTGG CATCAGAACT CCTGCAATGG 
ATTGAACAGA TGATCATTAC TCTCAATGAC CGAAAACTGG CAAACTCTCT AAGTGGTGTT 
CAGAACCAGC TGCAGGCCTT CAACACCTAC AGGACTGTAG AAAAGCCGCC CAAGTTCACA 
GAAAAGGGCA ATTTGGAAGT TCTTCTCTTT ACCATACAGA GTAAAATGAG AGCTAATAAT 
CAAAAAGTGT ATACGCCCAG AGAAGGAAAG CTTATATCTG ATATAAACAA GGCATGGGAG 
CGACTAGAAA AGGCAGAGCA TGAACGTGAG CTTGCCCTTC GCAATGAACT TATACGCCAG 
GAAAAATTAG AGCAGTTGGC TGCACGATTT GACCGTAAAG CAGCCATGAG GGAAACCTGG 
CTAAGTGAAA ATCAGAGACT TGTAGCTCAG GATAACTTCG GTGTGGACTT AACTGCGGTG 
GAGGCAGCGC TACGGAAACA TGAAGCTATT GAAACTGATA TATTGGCTTA CAGTGAGCGC 
GTGCAAGCAG TGAATGCAGT GGCTGATGAG CTGGAAGCAG AGAATTACCA TGATGTCCGG 
AGGATTTTGG CTCGGAAGTG CAATGTGATT CGTCTCTCTG ATTATTTAAG AGAGCTTGTC 
ACTGCCAGGA GGCAAAGGCT TCATATGAAC CTAGAATTGC AGAGGATGTT CCAGGATTTA 
CATTATCTCA TGGACTGGAC TGAAGATATG AAGGGCCGTC TGCAGTCAGA AGATTATGGC 
AAGCATCTTC ATGGCGTGGA AGACCTTCTC CAGACTCACA CTCTTGTGGA GGCAGACATT 
GCAGTTCAGG CAGAACGTGT GAAATCAGTT ACAGCTGTAG CTCAGAAATT TATGACAGAA 
GATGAAGGCT ACAAGCCTTG CGACCCAGAG CTAGTGCGCA ACCGGGTGCA TCTCTTGGAA 
CAGCGCTATG CAGAGCTGTG TAATCTTTCT GCGGCTCGGA GATCAAAGCT GGAAGAATCC 
CGCCGTCTCT GGAAATTTTT CTGGGACATG GGAGAGGAGG AAGCCTGGAT CCGTGAGCAG 
AGTAGATTAC TACTTTCTGA TGACTATGGG AAAGACCTTA CCAGCTCACT TAGGTTGATC 
AGCAAGCATA ATGCCTTCAG AGGTGAGATG AGTGGACACC GTGGGCCGTT GCAGCAGACT 
GTGGCTGAGG GTGAGGAACT AATATCCAAA GGTCACTTTG GTGCAGGAGA GATTGAGGAA 
AGGATAAAAG AAGTGAAAGG CCAGTGGACA GAGATGGAGG CCTTAGCTGA AGACAGGGAA 
AGGAGGCTAA GAGAGGCCTA CAGCTATTAT CAGTTTCAAG CTGATGCCAA TGATGCTGAG 
GCTTGGATCC TGGACACTTT ACGCCTTGTT TCTACCAGTG AAGTAGGACA TGATGAGTAT 
TCCACACAGA CACTGGTGAA AAAGCACAAA GAAATAGAAG AGGAGATCCT TAGCCACCGA 
CCAGCTATTG ATGCGCTGCA TGAACAGGCC CGTGGCCTTC CACCTGCTTA CTCTCATTCA 
CCTGAAGTTG ATGGAAGGTT ACCAGCCCTG GAGCAGCGCT ATGAGGAATT GGTGGCTCTT 
GCTGCTCACC GCAAGCAGGC TCTGCAAGAT TCTCTGGCTC TTCATAAAAT GTTTAGTGAA 
GCTGATGCTT GCCTTTTGTG GATTGATGAG AAAGAACAGT GGCTTAATGT CACAGAGATT 
CCAGAAAAAC TTGAGGACCT AGAAGTAGTT CAACAAAGGT TTGAAACTCT AGAGGCAGAG 
ATGAATAATT TGGCATCCCG GATTGCATCT GTAAATGAAG TTGCTGGACA GTTGCTGACT 
ACAGACCAGA GAAATGAAAA CAGCATCCGA GACATGCAAG ATCAACTTAA TTCTCGGTGG 
AATGAATTCC GCAAGCTTGT TGACCAAAAG AAGGGTGCTT TGGATTCTGC TCTGGATATA 
CAGAATTATC ATTTGGAGTG CAATGAGACT GTGTGCTGGA TGAGGGAGAA GACAAAGGTT 
ATTGAATCCA CACAGAGCCT AGGGAATGAT CTTGCAGGAG TAACAGCCCT CCAGAGAAAA 
CTTACTGGAA TGGAGCGTGA TCTGAAAGCC ATTCAGGGAA AAGTGGGTGA CCTGCGTTCA 
GAGGCAGAAA AGCTTGCAGA TGGGCACCCA CAGAAAGCAC CGGCCATAAT TGGCCGCTTA 
TCAGATATTG ATGAGACCTG GGGAGAACTA AAGGACACTA TGAAAAGAAG AGAAGAAACT 
TTAGGTGAAG CAAGCAAGCT GCAGGGATTT TTGAGAGACC TTGATGATTT CCAGAGCTGG 
TTGTCCCGGG CCCAGACAGC AGTGGCATCT GAGGATGTTC CAGCTGACCT AGCAGAAGCA 
GAGCGCCTTC TTGCACAACA TGAAGGCATA AAAAATGAGA TTGATCATTA TGGAGCTGAC 
TACCACAGAG TGAGAGATGT TGGTGAAGAA GTCACTCGTG GGCAAACAGA TGCCCAATAT 
ATGTTCTTGC AACAGAGACT GAAGGCGCTA GACACTGGCT GGAATGAGCT TGGACAGATG 
TGGGAAAACA GGCACCAGTT GTTGTCCCAG GGTCACAGCT TCCAGCAGTT TTTGAAAGAT 
ACAAAACAAG TGGAGGGGTT CCTTAATAAC CAGGAGTATG TCCTTTCCCA TACCGAAATG 
CCAAGTACGC TACAGGCAGC TGAAGCTGCC ATCAAACGCC ATGAAGATTT CCTCACAACC 
ATGGATGCCA ATGAAGAGAA GATACAGGGT CTTGTAGAAT CTGGGAGGAA GCTAATTGAG 
GAACAGAATG TCAATTCTGA GAAGATTCAA GAAAAAGTCA CTTCTATTGA TGAGAGACAC 
AAAAAGAACC AAAAAACTGC TAGCGAAGTA TTAACTCGTT TGAAGGACAA CCGTGATCTT 
CAACATTTCC TTCAAGACTG TCAAGAGCTC ACCCTCTGGA TCAATGAAAA AATGCTTACT 
GCTCAAGATG TTTCTTATGA TGAAGCTCGA AACCTGCATA GCAAGTGGCA AAAGCATCAG 
GCATTCATGG CAGAACTGGC CTCAAATAAA GACTGGCTTG ACAAAATAGA CAAGGAAGGG 
CAGCAGCTCA TGTCAGAAAA ACCGGAGCTT GAACCAGTAG TGAGAGAAAA ACTAGACACT 
CTGCATAAGC TGTGGAATGA CTTGGAATCT ACCACACAAA GTAAAGCTCA GTGTCTGTTT 
GATGCCAACA GGGCAGAGTT ATTCACACAA AGCTGTTCTG CTCTGGAGAG TTGGCTTCAA 
AACCTCGAGT CACAACTACA GTCAGATCAT TATGGCAAAG ATCTGACCAG TGTCAACATC 
CTATTAAATA AACAGCAGAT GCTGGAGAAC CAAATGGAAG TGAGGGAGAA GGAGCTAGAA 
GCTATACAGT CCCAGGCACA GGCCCTTGGA CAGGAGGATG CCAACATAGT GGAGGTTGAC 
TCAAAGCATA AGGATGTAGC AGATAAATTT TCCAAGCTGC GTGCTCCTCT AAACAAGAGA 
AGAAAATTAC TTCTAGCCTC CAAGGAAGGA CATCAGTTTA ACAGGGACCT GGAAGATGAA 
ATACTTTGGG TAAAGGAGAA GATGCCAATG GCAATCTCCA CTGATCATGG AAAAGATCTC 
CCGACAGTCC AGCTTCTTAT CAAAAAGAAT CAGACACTCC AAAAGGAAAT TTTGGGACAT 
CAACCACGGA TTGATGACAT CCTGGAGAGA GGCAAATCTA TGATCCAAGA CAGCTCTCTG 
GACCCTCAAG AGATAGAAGA AAGGCTTGGG CTACTGAAAA GCACATGGGA AGAGCTGGCA 
GCAGAAGGAG GAAAGCGCCA TGCCCGTTTA GAGGAATCTC TTGCTGCTCA GCAGTTTTAT 
GCTGATGCGG CAGAAGCTGA GGCCTGGATG GGAGAGCAAG AGTTACATAT GATGTCTGAA 
GAAAAAGCCA AGGATGAGAT TGGTGCCCAG GCTATGCTGA AGAAACACTT AGTAATGGAA 
CAGGCTCTTG ATGACTATGC AGACAACATC CATCAGCTTG CCAATTCAAG CCGCGACATG 
GTCTCCAGAG GTCATCCAGA AAGTGATCGT ATTACCCTTC GTCAAGGGCA AGTGGACAAG 
CTATATGCCA GTCTTAAAGA CCTGGCAGAG GAGAGACAAG GAAAGCTACA AGAGCACCAC 
AGACTTTGCC AACTCAAGAG AGATGTCGAA GACCTTGAGC ACTGGATCTC TGAACGTGAG 
GTGGTTGCAG CTTCTCATGA ACTTGGGCAA GATTATGAGC ATGTAACGAT GCTGAGAGAC 
AAATTCCGTG AGTTCTCCCG TGACACCAGT AACATTGGTC AAGAGCGGGT GGATGCTGTC 
AATCTGATGG CAGATGATTT AATTTCATCT GGACATTCGG AAAATGCTAC TGTAGCAGAT 
TGGAAAGACC AGCTAAATGA GGCATGGGCT GATCTTCTTG AGATGATCGA CACTCGAAGT 
CAGATGCTGG CAGCCTCATA TGAACTTCAC CGTTTCTACC AGGATGCAAA AGAAACCCTA 
GGTCAGATCC AAGACAAGCA GAAACAAATA CCAGAGGAGA CAGGCAGAGA TCTGAATACA 
GCAGAAGCCA TGCAGCGCAT GCACACCGCT TTTGAACATG ATATCCAAGC CCTGAGTGCA 
CAGGTGAAGC AAGTCCAGGA AGATGCTTCT CGCCTCCAAA AAGCTTATGC TGGTGAAAAG 
GCAGCAGACA TTCATCGCCA TGAGCAGGCA GTATCGGAAG CTTGGGGAGA GTTGCAGAAT 
GCTAGTGGTG CCAGGCGGCA GCTTCTTCTT GACACTGTGG AGAAATTCCG TTTCTTCAGA 
ATGGTGCGAG ATCTCATGTT ATGGATGGAT GATATAAACC TGCTAATTGA TGCACAGGAG 
AAGCCAAGAG ATGTATCCTC AGCTGATTTA GTCATCCAAA ACCACCTAGG CATCAAGGCA 
GAAGTGGATG CGCGAGCTGA CAGCTTTACT ACATGCATAG ATCTTGGCAA CTCTCTCCTA 
GAGAAGAATC ATTATGCCTC TGACAAGATT TCAGAGAAAC TGGAGCAATT GCAGGAAAGA 
AGGCAGGAGA CTGAAAACAA ATGGAAAGAT AAAATGGCAT GGCTGAGAGT TGTCCACGAA 
GTGCTGATGT TTGGAAGAGA TGCAAGTGTA GCTGAGGCTT GGCTGGCCAG CCAGGAGCCC 
ATTGTCCGGT CGTCAGAACT TGGTGGCAAT GTCGATGAAG TAGAATCCCT GATAAAAAGA 
CACGAAGCCT TTCAGAAAGC AGTTTCTACA TGGGATGAGA GATTTGTTCT TCTTGAAAAA 
CTAACCACGT TAGAAGAAAT TGAAATGCGT CGGAAGCGGG AAGAGGAAGA AAGAAAGAAA 
AGACCACCAA CACCACCTCT ACCAGTAGAA GAGCCTCGAG AGGAAGTTGT AAAAACCCAA 
GTTTCCCAGA CTATTGAAGG ACAGACTGAT CCAGCCAATA TACTTCAAGA AAGGATAAAT 
GGCATGCAGA ATTCATTCCA GGAGCAAGTA TCTGTTAATG GACTGTGCAC AGAGTCTGAT 
TCTCCACAGC CATCGTCCGA TACACAGAGG TTAGAAGACA GCATCTCTGA AAATCAGGGT 
CCGGAACAGT CAGATGTAAC CAATGGAGCC AGGACTGAGG TTATTACCAA AGATGGAAGT 
CCAACACCTT CTCCACAACT TGTGGCTAGG CCAACCTTCT CCCCGATAAA CCTCCAGTAT 
TCTACTTTGC CATCTAGCCC CACTGAACCA TTGGCATCTG AGCAAATGGA AGGAATGCTG 
CACCGCAAGC AGGAAATGGA TTCTCACAAC AAGAAGGCTG CGAACAGGTC CTGGCAGAAT 
GTTTACTGTG CACTGAAAAG GGGCAGTCTG GGTTTCTACA AGGATGCTAA AAGTGCAGCC 
AGTGGCGTTT CTTATCATTC GGAGCCCTCC GTCCAACTTA ATGGAGCCCA GTGCAGTGTT 
GCTCATGACT ACAAGAAACG CAAGAATGTC TTCAAGCTAA GCCTTCTTGA TGGCAAAGAA 
TATCTGTTCC AAGCTAAGGA TGAGGCTGAA ATGAGTAGTT GGATGCGAGT TATAAACGCC 
TCCCTAACTT CCTCCCCGGG AGACCCCTCA GACCATTCAC CGGGCATCAT GAAGGGTATG 
ACCCGGGCCA TGACAATGCC ACCAGTATCA CCCAACAGTG GTACAGAGGG ATCAGTCACA 
TTGCGCAACA AGGACGGGAA AGAAAAAGAC CGGGAAAAGA GATTCAGTTT CTTTAAGAAG 
AAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004913752.1
CDS	247..7452
Translation	MSSPLSPTDYDSLEIQQQYNDINNRWDLTDDDWDNENSSARLFERSRIKALADEREAVQKKTFTKWVNSHLARVTCRISDLYNDLRDGRMLIRLLEVLSGEQLPKPTKGRMRIHCLENVDKALQFLKEQKVHLENMGSHDIVDGNHRLTLGLIWTIILRFQIQDISVETEDNKEKKSAKDALLLWCQMKTAGYPNVNVHNFTTSWRDGLAFNAIVHKHRPDIIDFDTLKKSNAHHNLQNAFNVAERELGLTKLLDPEDVNVDQPDEKSIITYVATFYHYFSKMKALAVEGKRIGKVLDQAIEADQLIENYEGLASELLQWIEQMIITLNDRKLANSLSGVQNQLQAFNTYRTVEKPPKFTEKGNLEVLLFTIQSKMRANNQKVYTPREGKLISDINKAWERLEKAEHERELALRNELIRQEKLEQLAARFDRKAAMRETWLSENQRLVAQDNFGVDLTAVEAALRKHEAIETDILAYSERVQAVNAVADELEAENYHDVRRILARKCNVIRLSDYLRELVTARRQRLHMNLELQRMFQDLHYLMDWTEDMKGRLQSEDYGKHLHGVEDLLQTHTLVEADIAVQAERVKSVTAVAQKFMTEDEGYKPCDPELVRNRVHLLEQRYAELCNLSAARRSKLEESRRLWKFFWDMGEEEAWIREQSRLLLSDDYGKDLTSSLRLISKHNAFRGEMSGHRGPLQQTVAEGEELISKGHFGAGEIEERIKEVKGQWTEMEALAEDRERRLREAYSYYQFQADANDAEAWILDTLRLVSTSEVGHDEYSTQTLVKKHKEIEEEILSHRPAIDALHEQARGLPPAYSHSPEVDGRLPALEQRYEELVALAAHRKQALQDSLALHKMFSEADACLLWIDEKEQWLNVTEIPEKLEDLEVVQQRFETLEAEMNNLASRIASVNEVAGQLLTTDQRNENSIRDMQDQLNSRWNEFRKLVDQKKGALDSALDIQNYHLECNETVCWMREKTKVIESTQSLGNDLAGVTALQRKLTGMERDLKAIQGKVGDLRSEAEKLADGHPQKAPAIIGRLSDIDETWGELKDTMKRREETLGEASKLQGFLRDLDDFQSWLSRAQTAVASEDVPADLAEAERLLAQHEGIKNEIDHYGADYHRVRDVGEEVTRGQTDAQYMFLQQRLKALDTGWNELGQMWENRHQLLSQGHSFQQFLKDTKQVEGFLNNQEYVLSHTEMPSTLQAAEAAIKRHEDFLTTMDANEEKIQGLVESGRKLIEEQNVNSEKIQEKVTSIDERHKKNQKTASEVLTRLKDNRDLQHFLQDCQELTLWINEKMLTAQDVSYDEARNLHSKWQKHQAFMAELASNKDWLDKIDKEGQQLMSEKPELEPVVREKLDTLHKLWNDLESTTQSKAQCLFDANRAELFTQSCSALESWLQNLESQLQSDHYGKDLTSVNILLNKQQMLENQMEVREKELEAIQSQAQALGQEDANIVEVDSKHKDVADKFSKLRAPLNKRRKLLLASKEGHQFNRDLEDEILWVKEKMPMAISTDHGKDLPTVQLLIKKNQTLQKEILGHQPRIDDILERGKSMIQDSSLDPQEIEERLGLLKSTWEELAAEGGKRHARLEESLAAQQFYADAAEAEAWMGEQELHMMSEEKAKDEIGAQAMLKKHLVMEQALDDYADNIHQLANSSRDMVSRGHPESDRITLRQGQVDKLYASLKDLAEERQGKLQEHHRLCQLKRDVEDLEHWISEREVVAASHELGQDYEHVTMLRDKFREFSRDTSNIGQERVDAVNLMADDLISSGHSENATVADWKDQLNEAWADLLEMIDTRSQMLAASYELHRFYQDAKETLGQIQDKQKQIPEETGRDLNTAEAMQRMHTAFEHDIQALSAQVKQVQEDASRLQKAYAGEKAADIHRHEQAVSEAWGELQNASGARRQLLLDTVEKFRFFRMVRDLMLWMDDINLLIDAQEKPRDVSSADLVIQNHLGIKAEVDARADSFTTCIDLGNSLLEKNHYASDKISEKLEQLQERRQETENKWKDKMAWLRVVHEVLMFGRDASVAEAWLASQEPIVRSSELGGNVDEVESLIKRHEAFQKAVSTWDERFVLLEKLTTLEEIEMRRKREEEERKKRPPTPPLPVEEPREEVVKTQVSQTIEGQTDPANILQERINGMQNSFQEQVSVNGLCTESDSPQPSSDTQRLEDSISENQGPEQSDVTNGARTEVITKDGSPTPSPQLVARPTFSPINLQYSTLPSSPTEPLASEQMEGMLHRKQEMDSHNKKAANRSWQNVYCALKRGSLGFYKDAKSAASGVSYHSEPSVQLNGAQCSVAHDYKKRKNVFKLSLLDGKEYLFQAKDEAEMSSWMRVINASLTSSPGDPSDHSPGIMKGMTRAMTMPPVSPNSGTEGSVTLRNKDGKEKDREKRFSFFKKK

Target ORF information:

RefSeq Version	XM_004913695.3
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis spectrin, beta, non-erythrocytic 2 (sptbn2), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004913695.3

ORF Insert Sequence:

ATGAGCAGTC CACTGTCACC GACAGATTAT GACAGCCTGG AAATCCAGCA GCAGTATAAT 
GACATCAACA ATCGATGGGA TCTGACAGAT GATGATTGGG ACAATGAGAA CAGTTCTGCA 
AGACTTTTTG AGAGGTCCCG GATCAAAGCA CTTGCAGATG AAAGAGAAGC TGTGCAGAAG 
AAGACATTTA CTAAATGGGT CAACTCTCAC TTGGCGAGGG TCACCTGTAG AATTTCTGAT 
CTCTACAATG ATTTACGAGA TGGACGGATG TTAATTCGAC TGTTAGAAGT GCTATCTGGA 
GAGCAGCTGC CAAAACCCAC AAAAGGTCGA ATGCGAATCC ACTGTTTGGA AAATGTAGAC 
AAAGCCCTTC AATTTTTAAA AGAGCAGAAG GTACATTTGG AGAACATGGG ATCACATGAC 
ATTGTGGATG GAAACCATCG ATTAACCCTA GGGTTAATCT GGACTATTAT TTTAAGGTTT 
CAGATCCAAG ATATCAGTGT AGAGACAGAA GATAATAAAG AAAAGAAGTC TGCTAAAGAT 
GCCTTACTGC TTTGGTGCCA GATGAAGACT GCTGGGTATC CAAATGTCAA TGTTCACAAC 
TTTACCACCA GCTGGCGAGA TGGACTTGCG TTTAATGCCA TTGTACACAA GCACAGACCA 
GATATAATTG ACTTTGACAC ACTGAAGAAG TCTAATGCAC ACCACAACCT CCAGAATGCC 
TTCAATGTAG CTGAAAGAGA GTTAGGTCTT ACTAAACTTC TGGATCCTGA AGATGTTAAT 
GTGGATCAGC CTGATGAGAA ATCCATCATA ACCTATGTGG CCACATTTTA TCACTATTTT 
TCCAAAATGA AAGCCCTAGC TGTGGAAGGA AAGCGTATTG GAAAGGTACT TGACCAAGCT 
ATTGAAGCTG ATCAGTTAAT TGAGAACTAT GAAGGACTGG CATCAGAACT CCTGCAATGG 
ATTGAACAGA TGATCATTAC TCTCAATGAC CGAAAACTGG CAAACTCTCT AAGTGGTGTT 
CAGAACCAGC TGCAGGCCTT CAACACCTAC AGGACTGTAG AAAAGCCGCC CAAGTTCACA 
GAAAAGGGCA ATTTGGAAGT TCTTCTCTTT ACCATACAGA GTAAAATGAG AGCTAATAAT 
CAAAAAGTGT ATACGCCCAG AGAAGGAAAG CTTATATCTG ATATAAACAA GGCATGGGAG 
CGACTAGAAA AGGCAGAGCA TGAACGTGAG CTTGCCCTTC GCAATGAACT TATACGCCAG 
GAAAAATTAG AGCAGTTGGC TGCACGATTT GACCGTAAAG CAGCCATGAG GGAAACCTGG 
CTAAGTGAAA ATCAGAGACT TGTAGCTCAG GATAACTTCG GTGTGGACTT AACTGCGGTG 
GAGGCAGCGC TACGGAAACA TGAAGCTATT GAAACTGATA TATTGGCTTA CAGTGAGCGC 
GTGCAAGCAG TGAATGCAGT GGCTGATGAG CTGGAAGCAG AGAATTACCA TGATGTCCGG 
AGGATTTTGG CTCGGAAGTG CAATGTGATT CGTCTCTCTG ATTATTTAAG AGAGCTTGTC 
ACTGCCAGGA GGCAAAGGCT TCATATGAAC CTAGAATTGC AGAGGATGTT CCAGGATTTA 
CATTATCTCA TGGACTGGAC TGAAGATATG AAGGGCCGTC TGCAGTCAGA AGATTATGGC 
AAGCATCTTC ATGGCGTGGA AGACCTTCTC CAGACTCACA CTCTTGTGGA GGCAGACATT 
GCAGTTCAGG CAGAACGTGT GAAATCAGTT ACAGCTGTAG CTCAGAAATT TATGACAGAA 
GATGAAGGCT ACAAGCCTTG CGACCCAGAG CTAGTGCGCA ACCGGGTGCA TCTCTTGGAA 
CAGCGCTATG CAGAGCTGTG TAATCTTTCT GCGGCTCGGA GATCAAAGCT GGAAGAATCC 
CGCCGTCTCT GGAAATTTTT CTGGGACATG GGAGAGGAGG AAGCCTGGAT CCGTGAGCAG 
AGTAGATTAC TACTTTCTGA TGACTATGGG AAAGACCTTA CCAGCTCACT TAGGTTGATC 
AGCAAGCATA ATGCCTTCAG AGGTGAGATG AGTGGACACC GTGGGCCGTT GCAGCAGACT 
GTGGCTGAGG GTGAGGAACT AATATCCAAA GGTCACTTTG GTGCAGGAGA GATTGAGGAA 
AGGATAAAAG AAGTGAAAGG CCAGTGGACA GAGATGGAGG CCTTAGCTGA AGACAGGGAA 
AGGAGGCTAA GAGAGGCCTA CAGCTATTAT CAGTTTCAAG CTGATGCCAA TGATGCTGAG 
GCTTGGATCC TGGACACTTT ACGCCTTGTT TCTACCAGTG AAGTAGGACA TGATGAGTAT 
TCCACACAGA CACTGGTGAA AAAGCACAAA GAAATAGAAG AGGAGATCCT TAGCCACCGA 
CCAGCTATTG ATGCGCTGCA TGAACAGGCC CGTGGCCTTC CACCTGCTTA CTCTCATTCA 
CCTGAAGTTG ATGGAAGGTT ACCAGCCCTG GAGCAGCGCT ATGAGGAATT GGTGGCTCTT 
GCTGCTCACC GCAAGCAGGC TCTGCAAGAT TCTCTGGCTC TTCATAAAAT GTTTAGTGAA 
GCTGATGCTT GCCTTTTGTG GATTGATGAG AAAGAACAGT GGCTTAATGT CACAGAGATT 
CCAGAAAAAC TTGAGGACCT AGAAGTAGTT CAACAAAGGT TTGAAACTCT AGAGGCAGAG 
ATGAATAATT TGGCATCCCG GATTGCATCT GTAAATGAAG TTGCTGGACA GTTGCTGACT 
ACAGACCAGA GAAATGAAAA CAGCATCCGA GACATGCAAG ATCAACTTAA TTCTCGGTGG 
AATGAATTCC GCAAGCTTGT TGACCAAAAG AAGGGTGCTT TGGATTCTGC TCTGGATATA 
CAGAATTATC ATTTGGAGTG CAATGAGACT GTGTGCTGGA TGAGGGAGAA GACAAAGGTT 
ATTGAATCCA CACAGAGCCT AGGGAATGAT CTTGCAGGAG TAACAGCCCT CCAGAGAAAA 
CTTACTGGAA TGGAGCGTGA TCTGAAAGCC ATTCAGGGAA AAGTGGGTGA CCTGCGTTCA 
GAGGCAGAAA AGCTTGCAGA TGGGCACCCA CAGAAAGCAC CGGCCATAAT TGGCCGCTTA 
TCAGATATTG ATGAGACCTG GGGAGAACTA AAGGACACTA TGAAAAGAAG AGAAGAAACT 
TTAGGTGAAG CAAGCAAGCT GCAGGGATTT TTGAGAGACC TTGATGATTT CCAGAGCTGG 
TTGTCCCGGG CCCAGACAGC AGTGGCATCT GAGGATGTTC CAGCTGACCT AGCAGAAGCA 
GAGCGCCTTC TTGCACAACA TGAAGGCATA AAAAATGAGA TTGATCATTA TGGAGCTGAC 
TACCACAGAG TGAGAGATGT TGGTGAAGAA GTCACTCGTG GGCAAACAGA TGCCCAATAT 
ATGTTCTTGC AACAGAGACT GAAGGCGCTA GACACTGGCT GGAATGAGCT TGGACAGATG 
TGGGAAAACA GGCACCAGTT GTTGTCCCAG GGTCACAGCT TCCAGCAGTT TTTGAAAGAT 
ACAAAACAAG TGGAGGGGTT CCTTAATAAC CAGGAGTATG TCCTTTCCCA TACCGAAATG 
CCAAGTACGC TACAGGCAGC TGAAGCTGCC ATCAAACGCC ATGAAGATTT CCTCACAACC 
ATGGATGCCA ATGAAGAGAA GATACAGGGT CTTGTAGAAT CTGGGAGGAA GCTAATTGAG 
GAACAGAATG TCAATTCTGA GAAGATTCAA GAAAAAGTCA CTTCTATTGA TGAGAGACAC 
AAAAAGAACC AAAAAACTGC TAGCGAAGTA TTAACTCGTT TGAAGGACAA CCGTGATCTT 
CAACATTTCC TTCAAGACTG TCAAGAGCTC ACCCTCTGGA TCAATGAAAA AATGCTTACT 
GCTCAAGATG TTTCTTATGA TGAAGCTCGA AACCTGCATA GCAAGTGGCA AAAGCATCAG 
GCATTCATGG CAGAACTGGC CTCAAATAAA GACTGGCTTG ACAAAATAGA CAAGGAAGGG 
CAGCAGCTCA TGTCAGAAAA ACCGGAGCTT GAACCAGTAG TGAGAGAAAA ACTAGACACT 
CTGCATAAGC TGTGGAATGA CTTGGAATCT ACCACACAAA GTAAAGCTCA GTGTCTGTTT 
GATGCCAACA GGGCAGAGTT ATTCACACAA AGCTGTTCTG CTCTGGAGAG TTGGCTTCAA 
AACCTCGAGT CACAACTACA GTCAGATCAT TATGGCAAAG ATCTGACCAG TGTCAACATC 
CTATTAAATA AACAGCAGAT GCTGGAGAAC CAAATGGAAG TGAGGGAGAA GGAGCTAGAA 
GCTATACAGT CCCAGGCACA GGCCCTTGGA CAGGAGGATG CCAACATAGT GGAGGTTGAC 
TCAAAGCATA AGGATGTAGC AGATAAATTT TCCAAGCTGC GTGCTCCTCT AAACAAGAGA 
AGAAAATTAC TTCTAGCCTC CAAGGAAGGA CATCAGTTTA ACAGGGACCT GGAAGATGAA 
ATACTTTGGG TAAAGGAGAA GATGCCAATG GCAATCTCCA CTGATCATGG AAAAGATCTC 
CCGACAGTCC AGCTTCTTAT CAAAAAGAAT CAGACACTCC AAAAGGAAAT TTTGGGACAT 
CAACCACGGA TTGATGACAT CCTGGAGAGA GGCAAATCTA TGATCCAAGA CAGCTCTCTG 
GACCCTCAAG AGATAGAAGA AAGGCTTGGG CTACTGAAAA GCACATGGGA AGAGCTGGCA 
GCAGAAGGAG GAAAGCGCCA TGCCCGTTTA GAGGAATCTC TTGCTGCTCA GCAGTTTTAT 
GCTGATGCGG CAGAAGCTGA GGCCTGGATG GGAGAGCAAG AGTTACATAT GATGTCTGAA 
GAAAAAGCCA AGGATGAGAT TGGTGCCCAG GCTATGCTGA AGAAACACTT AGTAATGGAA 
CAGGCTCTTG ATGACTATGC AGACAACATC CATCAGCTTG CCAATTCAAG CCGCGACATG 
GTCTCCAGAG GTCATCCAGA AAGTGATCGT ATTACCCTTC GTCAAGGGCA AGTGGACAAG 
CTATATGCCA GTCTTAAAGA CCTGGCAGAG GAGAGACAAG GAAAGCTACA AGAGCACCAC 
AGACTTTGCC AACTCAAGAG AGATGTCGAA GACCTTGAGC ACTGGATCTC TGAACGTGAG 
GTGGTTGCAG CTTCTCATGA ACTTGGGCAA GATTATGAGC ATGTAACGAT GCTGAGAGAC 
AAATTCCGTG AGTTCTCCCG TGACACCAGT AACATTGGTC AAGAGCGGGT GGATGCTGTC 
AATCTGATGG CAGATGATTT AATTTCATCT GGACATTCGG AAAATGCTAC TGTAGCAGAT 
TGGAAAGACC AGCTAAATGA GGCATGGGCT GATCTTCTTG AGATGATCGA CACTCGAAGT 
CAGATGCTGG CAGCCTCATA TGAACTTCAC CGTTTCTACC AGGATGCAAA AGAAACCCTA 
GGTCAGATCC AAGACAAGCA GAAACAAATA CCAGAGGAGA CAGGCAGAGA TCTGAATACA 
GCAGAAGCCA TGCAGCGCAT GCACACCGCT TTTGAACATG ATATCCAAGC CCTGAGTGCA 
CAGGTGAAGC AAGTCCAGGA AGATGCTTCT CGCCTCCAAA AAGCTTATGC TGGTGAAAAG 
GCAGCAGACA TTCATCGCCA TGAGCAGGCA GTATCGGAAG CTTGGGGAGA GTTGCAGAAT 
GCTAGTGGTG CCAGGCGGCA GCTTCTTCTT GACACTGTGG AGAAATTCCG TTTCTTCAGA 
ATGGTGCGAG ATCTCATGTT ATGGATGGAT GATATAAACC TGCTAATTGA TGCACAGGAG 
AAGCCAAGAG ATGTATCCTC AGCTGATTTA GTCATCCAAA ACCACCTAGG CATCAAGGCA 
GAAGTGGATG CGCGAGCTGA CAGCTTTACT ACATGCATAG ATCTTGGCAA CTCTCTCCTA 
GAGAAGAATC ATTATGCCTC TGACAAGATT TCAGAGAAAC TGGAGCAATT GCAGGAAAGA 
AGGCAGGAGA CTGAAAACAA ATGGAAAGAT AAAATGGCAT GGCTGAGAGT TGTCCACGAA 
GTGCTGATGT TTGGAAGAGA TGCAAGTGTA GCTGAGGCTT GGCTGGCCAG CCAGGAGCCC 
ATTGTCCGGT CGTCAGAACT TGGTGGCAAT GTCGATGAAG TAGAATCCCT GATAAAAAGA 
CACGAAGCCT TTCAGAAAGC AGTTTCTACA TGGGATGAGA GATTTGTTCT TCTTGAAAAA 
CTAACCACGT TAGAAGAAAT TGAAATGCGT CGGAAGCGGG AAGAGGAAGA AAGAAAGAAA 
AGACCACCAA CACCACCTCT ACCAGTAGAA GAGCCTCGAG AGGAAGTTGT AAAAACCCAA 
GTTTCCCAGA CTATTGAAGG ACAGACTGAT CCAGCCAATA TACTTCAAGA AAGGATAAAT 
GGCATGCAGA ATTCATTCCA GGAGCAAGTA TCTGTTAATG GACTGTGCAC AGAGTCTGAT 
TCTCCACAGC CATCGTCCGA TACACAGAGG TTAGAAGACA GCATCTCTGA AAATCAGGGT 
CCGGAACAGT CAGATGTAAC CAATGGAGCC AGGACTGAGG TTATTACCAA AGATGGAAGT 
CCAACACCTT CTCCACAACT TGTGGCTAGG CCAACCTTCT CCCCGATAAA CCTCCAGTAT 
TCTACTTTGC CATCTAGCCC CACTGAACCA TTGGCATCTG AGCAAATGGA AGGAATGCTG 
CACCGCAAGC AGGAAATGGA TTCTCACAAC AAGAAGGCTG CGAACAGGTC CTGGCAGAAT 
GTTTACTGTG CACTGAAAAG GGGCAGTCTG GGTTTCTACA AGGATGCTAA AAGTGCAGCC 
AGTGGCGTTT CTTATCATTC GGAGCCCTCC GTCCAACTTA ATGGAGCCCA GTGCAGTGTT 
GCTCATGACT ACAAGAAACG CAAGAATGTC TTCAAGCTAA GCCTTCTTGA TGGCAAAGAA 
TATCTGTTCC AAGCTAAGGA TGAGGCTGAA ATGAGTAGTT GGATGCGAGT TATAAACGCC 
TCCCTAACTT CCTCCCCGGG AGACCCCTCA GACCATTCAC CGGGCATCAT GAAGGGTATG 
ACCCGGGCCA TGACAATGCC ACCAGTATCA CCCAACAGTG GTACAGAGGG ATCAGTCACA 
TTGCGCAACA AGGACGGGAA AGAAAAAGAC CGGGAAAAGA GATTCAGTTT CTTTAAGAAG 
AAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

sptbn2 ( XM_012962405.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012817859.1 Xenopus tropicalis spectrin, beta, non-erythrocytic 2 (sptbn2), transcript variant X3, mRNA.

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CloneID	OXa33551
Clone ID Related Accession (Same CDS sequence)	XM_012962405.2
Accession Version	XM_012962405.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7314bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1473177600000
Organism	Xenopus tropicalis(tropical clawed frog)
Product	spectrin beta chain, non-erythrocytic 2 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030680.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012962405.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCAGTC CACTGTCACC GACAGATTAT GACAGCCTGG AAATCCAGCA GCAGTATAAT 
GACATCAACA ATCGATGGGA TCTGACAGAT GATGATTGGG ACAATGAGAA CAGTTCTGCA 
AGACTTTTTG AGAGGTCCCG GATCAAAGCA CTTGCAGAAA TGGATTTTGT CCCCCGATCT 
GGCTCCCCTC TCCTATCCCC TGCGGCTTTT GTAAATGCAG TGCAATACGC TAACGTGCTG 
GAAGGAAGGT TCAAGCAGCT GCAAGATGAA AGAGAAGCTG TGCAGAAGAA GACATTTACT 
AAATGGGTCA ACTCTCACTT GGCGAGGGTC ACCTGTAGAA TTTCTGATCT CTACAATGAT 
TTACGAGATG GACGGATGTT AATTCGACTG TTAGAAGTGC TATCTGGAGA GCAGCTGCCA 
AAACCCACAA AAGGTCGAAT GCGAATCCAC TGTTTGGAAA ATGTAGACAA AGCCCTTCAA 
TTTTTAAAAG AGCAGAAGGT ACATTTGGAG AACATGGGAT CACATGACAT TGTGGATGGA 
AACCATCGAT TAACCCTAGG GTTAATCTGG ACTATTATTT TAAGGTTTCA GATCCAAGAT 
ATCAGTGTAG AGACAGAAGA TAATAAAGAA AAGAAGTCTG CTAAAGATGC CTTACTGCTT 
TGGTGCCAGA TGAAGACTGC TGGGTATCCA AATGTCAATG TTCACAACTT TACCACCAGC 
TGGCGAGATG GACTTGCGTT TAATGCCATT GTACACAAGC ACAGACCAGA TATAATTGAC 
TTTGACACAC TGAAGAAGTC TAATGCACAC CACAACCTCC AGAATGCCTT CAATGTAGCT 
GAAAGAGAGT TAGGTCTTAC TAAACTTCTG GATCCTGAAG ATGTTAATGT GGATCAGCCT 
GATGAGAAAT CCATCATAAC CTATGTGGCC ACATTTTATC ACTATTTTTC CAAAATGAAA 
GCCCTAGCTG TGGAAGGAAA GCGTATTGGA AAGGTACTTG ACCAAGCTAT TGAAGCTGAT 
CAGTTAATTG AGAACTATGA AGGACTGGCA TCAGAACTCC TGCAATGGAT TGAACAGATG 
ATCATTACTC TCAATGACCG AAAACTGGCA AACTCTCTAA GTGGTGTTCA GAACCAGCTG 
CAGGCCTTCA ACACCTACAG GACTGTAGAA AAGCCGCCCA AGTTCACAGA AAAGGGCAAT 
TTGGAAGTTC TTCTCTTTAC CATACAGAGT AAAATGAGAG CTAATAATCA AAAAGTGTAT 
ACGCCCAGAG AAGGAAAGCT TATATCTGAT ATAAACAAGG CATGGGAGCG ACTAGAAAAG 
GCAGAGCATG AACGTGAGCT TGCCCTTCGC AATGAACTTA TACGCCAGGA AAAATTAGAG 
CAGTTGGCTG CACGATTTGA CCGTAAAGCA GCCATGAGGG AAACCTGGCT AAGTGAAAAT 
CAGAGACTTG TAGCTCAGGA TAACTTCGGT GTGGACTTAA CTGCGGTGGA GGCAGCGCTA 
CGGAAACATG AAGCTATTGA AACTGATATA TTGGCTTACA GTGAGCGCGT GCAAGCAGTG 
AATGCAGTGG CTGATGAGCT GGAAGCAGAG AATTACCATG ATGTCCGGAG GATTTTGGCT 
CGGAAGTGCA ATGTGATTCG TCTCTCTGAT TATTTAAGAG AGCTTGTCAC TGCCAGGAGG 
CAAAGGCTTC ATATGAACCT AGAATTGCAG AGGATGTTCC AGGATTTACA TTATCTCATG 
GACTGGACTG AAGATATGAA GGGCCGTCTG CAGTCAGAAG ATTATGGCAA GCATCTTCAT 
GGCGTGGAAG ACCTTCTCCA GACTCACACT CTTGTGGAGG CAGACATTGC AGTTCAGGCA 
GAACGTGTGA AATCAGTTAC AGCTGTAGCT CAGAAATTTA TGACAGAAGA TGAAGGCTAC 
AAGCCTTGCG ACCCAGAGCT AGTGCGCAAC CGGGTGCATC TCTTGGAACA GCGCTATGCA 
GAGCTGTGTA ATCTTTCTGC GGCTCGGAGA TCAAAGCTGG AAGAATCCCG CCGTCTCTGG 
AAATTTTTCT GGGACATGGG AGAGGAGGAA GCCTGGATCC GTGAGCAGAG TAGATTACTA 
CTTTCTGATG ACTATGGGAA AGACCTTACC AGCTCACTTA GGTTGATCAG CAAGCATAAT 
GCCTTCAGAG GTGAGATGAG TGGACACCGT GGGCCGTTGC AGCAGACTGT GGCTGAGGGT 
GAGGAACTAA TATCCAAAGG TCACTTTGGT GCAGGAGAGA TTGAGGAAAG GATAAAAGAA 
GTGAAAGGCC AGTGGACAGA GATGGAGGCC TTAGCTGAAG ACAGGGAAAG GAGGCTAAGA 
GAGGCCTACA GCTATTATCA GTTTCAAGCT GATGCCAATG ATGCTGAGGC TTGGATCCTG 
GACACTTTAC GCCTTGTTTC TACCAGTGAA GTAGGACATG ATGAGTATTC CACACAGACA 
CTGGTGAAAA AGCACAAAGA AATAGAAGAG GAGATCCTTA GCCACCGACC AGCTATTGAT 
GCGCTGCATG AACAGGCCCG TGGCCTTCCA CCTGCTTACT CTCATTCACC TGAAGTTGAT 
GGAAGGTTAC CAGCCCTGGA GCAGCGCTAT GAGGAATTGG TGGCTCTTGC TGCTCACCGC 
AAGCAGGCTC TGCAAGATTC TCTGGCTCTT CATAAAATGT TTAGTGAAGC TGATGCTTGC 
CTTTTGTGGA TTGATGAGAA AGAACAGTGG CTTAATGTCA CAGAGATTCC AGAAAAACTT 
GAGGACCTAG AAGTAGTTCA ACAAAGGTTT GAAACTCTAG AGGCAGAGAT GAATAATTTG 
GCATCCCGGA TTGCATCTGT AAATGAAGTT GCTGGACAGT TGCTGACTAC AGACCAGAGA 
AATGAAAACA GCATCCGAGA CATGCAAGAT CAACTTAATT CTCGGTGGAA TGAATTCCGC 
AAGCTTGTTG ACCAAAAGAA GGGTGCTTTG GATTCTGCTC TGGATATACA GAATTATCAT 
TTGGAGTGCA ATGAGACTGT GTGCTGGATG AGGGAGAAGA CAAAGGTTAT TGAATCCACA 
CAGAGCCTAG GGAATGATCT TGCAGGAGTA ACAGCCCTCC AGAGAAAACT TACTGGAATG 
GAGCGTGATC TGAAAGCCAT TCAGGGAAAA GTGGGTGACC TGCGTTCAGA GGCAGAAAAG 
CTTGCAGATG GGCACCCACA GAAAGCACCG GCCATAATTG GCCGCTTATC AGATATTGAT 
GAGACCTGGG GAGAACTAAA GGACACTATG AAAAGAAGAG AAGAAACTTT AGGTGAAGCA 
AGCAAGCTGC AGGGATTTTT GAGAGACCTT GATGATTTCC AGAGCTGGTT GTCCCGGGCC 
CAGACAGCAG TGGCATCTGA GGATGTTCCA GCTGACCTAG CAGAAGCAGA GCGCCTTCTT 
GCACAACATG AAGGCATAAA AAATGAGATT GATCATTATG GAGCTGACTA CCACAGAGTG 
AGAGATGTTG GTGAAGAAGT CACTCGTGGG CAAACAGATG CCCAATATAT GTTCTTGCAA 
CAGAGACTGA AGGCGCTAGA CACTGGCTGG AATGAGCTTG GACAGATGTG GGAAAACAGG 
CACCAGTTGT TGTCCCAGGG TCACAGCTTC CAGCAGTTTT TGAAAGATAC AAAACAAGTG 
GAGGGGTTCC TTAATAACCA GGAGTATGTC CTTTCCCATA CCGAAATGCC AAGTACGCTA 
CAGGCAGCTG AAGCTGCCAT CAAACGCCAT GAAGATTTCC TCACAACCAT GGATGCCAAT 
GAAGAGAAGA TACAGGGTCT TGTAGAATCT GGGAGGAAGC TAATTGAGGA ACAGAATGTC 
AATTCTGAGA AGATTCAAGA AAAAGTCACT TCTATTGATG AGAGACACAA AAAGAACCAA 
AAAACTGCTA GCGAAGTATT AACTCGTTTG AAGGACAACC GTGATCTTCA ACATTTCCTT 
CAAGACTGTC AAGAGCTCAC CCTCTGGATC AATGAAAAAA TGCTTACTGC TCAAGATGTT 
TCTTATGATG AAGCTCGAAA CCTGCATAGC AAGTGGCAAA AGCATCAGGC ATTCATGGCA 
GAACTGGCCT CAAATAAAGA CTGGCTTGAC AAAATAGACA AGGAAGGGCA GCAGCTCATG 
TCAGAAAAAC CGGAGCTTGA ACCAGTAGTG AGAGAAAAAC TAGACACTCT GCATAAGCTG 
TGGAATGACT TGGAATCTAC CACACAAAGT AAAGCTCAGT GTCTGTTTGA TGCCAACAGG 
GCAGAGTTAT TCACACAAAG CTGTTCTGCT CTGGAGAGTT GGCTTCAAAA CCTCGAGTCA 
CAACTACAGT CAGATCATTA TGGCAAAGAT CTGACCAGTG TCAACATCCT ATTAAATAAA 
CAGCAGATGC TGGAGAACCA AATGGAAGTG AGGGAGAAGG AGCTAGAAGC TATACAGTCC 
CAGGCACAGG CCCTTGGACA GGAGGATGCC AACATAGTGG AGGTTGACTC AAAGCATAAG 
GATGTAGCAG ATAAATTTTC CAAGCTGCGT GCTCCTCTAA ACAAGAGAAG AAAATTACTT 
CTAGCCTCCA AGGAAGGACA TCAGTTTAAC AGGGACCTGG AAGATGAAAT ACTTTGGGTA 
AAGGAGAAGA TGCCAATGGC AATCTCCACT GATCATGGAA AAGATCTCCC GACAGTCCAG 
CTTCTTATCA AAAAGAATCA GACACTCCAA AAGGAAATTT TGGGACATCA ACCACGGATT 
GATGACATCC TGGAGAGAGG CAAATCTATG ATCCAAGACA GCTCTCTGGA CCCTCAAGAG 
ATAGAAGAAA GGCTTGGGCT ACTGAAAAGC ACATGGGAAG AGCTGGCAGC AGAAGGAGGA 
AAGCGCCATG CCCGTTTAGA GGAATCTCTT GCTGCTCAGC AGTTTTATGC TGATGCGGCA 
GAAGCTGAGG CCTGGATGGG AGAGCAAGAG TTACATATGA TGTCTGAAGA AAAAGCCAAG 
GATGAGATTG GTGCCCAGGC TATGCTGAAG AAACACTTAG TAATGGAACA GGCTCTTGAT 
GACTATGCAG ACAACATCCA TCAGCTTGCC AATTCAAGCC GCGACATGGT CTCCAGAGGT 
CATCCAGAAA GTGATCGTAT TACCCTTCGT CAAGGGCAAG TGGACAAGCT ATATGCCAGT 
CTTAAAGACC TGGCAGAGGA GAGACAAGGA AAGCTACAAG AGCACCACAG ACTTTGCCAA 
CTCAAGAGAG ATGTCGAAGA CCTTGAGCAC TGGATCTCTG AACGTGAGGT GGTTGCAGCT 
TCTCATGAAC TTGGGCAAGA TTATGAGCAT GTAACGATGC TGAGAGACAA ATTCCGTGAG 
TTCTCCCGTG ACACCAGTAA CATTGGTCAA GAGCGGGTGG ATGCTGTCAA TCTGATGGCA 
GATGATTTAA TTTCATCTGG ACATTCGGAA AATGCTACTG TAGCAGATTG GAAAGACCAG 
CTAAATGAGG CATGGGCTGA TCTTCTTGAG ATGATCGACA CTCGAAGTCA GATGCTGGCA 
GCCTCATATG AACTTCACCG TTTCTACCAG GATGCAAAAG AAACCCTAGG TCAGATCCAA 
GACAAGCAGA AACAAATACC AGAGGAGACA GGCAGAGATC TGAATACAGC AGAAGCCATG 
CAGCGCATGC ACACCGCTTT TGAACATGAT ATCCAAGCCC TGAGTGCACA GGTGAAGCAA 
GTCCAGGAAG ATGCTTCTCG CCTCCAAAAA GCTTATGCTG GTGAAAAGGC AGCAGACATT 
CATCGCCATG AGCAGGCAGT ATCGGAAGCT TGGGGAGAGT TGCAGAATGC TAGTGGTGCC 
AGGCGGCAGC TTCTTCTTGA CACTGTGGAG AAATTCCGTT TCTTCAGAAT GGTGCGAGAT 
CTCATGTTAT GGATGGATGA TATAAACCTG CTAATTGATG CACAGGAGAA GCCAAGAGAT 
GTATCCTCAG CTGATTTAGT CATCCAAAAC CACCTAGGCA TCAAGGCAGA AGTGGATGCG 
CGAGCTGACA GCTTTACTAC ATGCATAGAT CTTGGCAACT CTCTCCTAGA GAAGAATCAT 
TATGCCTCTG ACAAGATTTC AGAGAAACTG GAGCAATTGC AGGAAAGAAG GCAGGAGACT 
GAAAACAAAT GGAAAGATAA AATGGCATGG CTGAGAGTTG TCCACGAAGT GCTGATGTTT 
GGAAGAGATG CAAGTGTAGC TGAGGCTTGG CTGGCCAGCC AGGAGCCCAT TGTCCGGTCG 
TCAGAACTTG GTGGCAATGT CGATGAAGTA GAATCCCTGA TAAAAAGACA CGAAGCCTTT 
CAGAAAGCAG TTTCTACATG GGATGAGAGA TTTGTTCTTC TTGAAAAACT AACCACGTTA 
GAAGAAATTG AAATGCGTCG GAAGCGGGAA GAGGAAGAAA GAAAGAAAAG ACCACCAACA 
CCACCTCTAC CAGTAGAAGA GCCTCGAGAG GAAGTTGTAA AAACCCAAGT TTCCCAGACT 
ATTGAAGGAC AGACTGATCC AGCCAATATA CTTCAAGAAA GGATAAATGG CATGCAGAAT 
TCATTCCAGG AGCAAGTATC TGTTAATGGA CTGTGCACAG AGTCTGATTC TCCACAGCCA 
TCGTCCGATA CACAGAGGTT AGAAGACAGC ATCTCTGAAA ATCAGGGTCC GGAACAGTCA 
GATGTAACCA ATGGAGCCAG GACTGAGGTT ATTACCAAAG ATGGAAGTCC AACACCTTCT 
CCACAACTTG TGGCTAGGCC AACCTTCTCC CCGATAAACC TCCAGTATTC TACTTTGCCA 
TCTAGCCCCA CTGAACCATT GGCATCTGAG CAAATGGAAG GAATGCTGCA CCGCAAGCAG 
GAAATGGATT CTCACAACAA GAAGGCTGCG AACAGGTCCT GGCAGAATGT TTACTGTGCA 
CTGAAAAGGG GCAGTCTGGG TTTCTACAAG GATGCTAAAA GTGCAGCCAG TGGCGTTTCT 
TATCATTCGG AGCCCTCCGT CCAACTTAAT GGAGCCCAGT GCAGTGTTGC TCATGACTAC 
AAGAAACGCA AGAATGTCTT CAAGCTAAGC CTTCTTGATG GCAAAGAATA TCTGTTCCAA 
GCTAAGGATG AGGCTGAAAT GAGTAGTTGG ATGCGAGTTA TAAACGCCTC CCTAACTTCC 
TCCCCGGGAG ACCCCTCAGA CCATTCACCG GGCATCATGA AGGGTATGAC CCGGGCCATG 
ACAATGCCAC CAGTATCACC CAACAGTGGT ACAGAGGGAT CAGTCACATT GCGCAACAAG 
GACGGGAAAG AAAAAGACCG GGAAAAGAGA TTCAGTTTCT TTAAGAAGAA GTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012817859.1
CDS	66..7379
Translation	MSSPLSPTDYDSLEIQQQYNDINNRWDLTDDDWDNENSSARLFERSRIKALAEMDFVPRSGSPLLSPAAFVNAVQYANVLEGRFKQLQDEREAVQKKTFTKWVNSHLARVTCRISDLYNDLRDGRMLIRLLEVLSGEQLPKPTKGRMRIHCLENVDKALQFLKEQKVHLENMGSHDIVDGNHRLTLGLIWTIILRFQIQDISVETEDNKEKKSAKDALLLWCQMKTAGYPNVNVHNFTTSWRDGLAFNAIVHKHRPDIIDFDTLKKSNAHHNLQNAFNVAERELGLTKLLDPEDVNVDQPDEKSIITYVATFYHYFSKMKALAVEGKRIGKVLDQAIEADQLIENYEGLASELLQWIEQMIITLNDRKLANSLSGVQNQLQAFNTYRTVEKPPKFTEKGNLEVLLFTIQSKMRANNQKVYTPREGKLISDINKAWERLEKAEHERELALRNELIRQEKLEQLAARFDRKAAMRETWLSENQRLVAQDNFGVDLTAVEAALRKHEAIETDILAYSERVQAVNAVADELEAENYHDVRRILARKCNVIRLSDYLRELVTARRQRLHMNLELQRMFQDLHYLMDWTEDMKGRLQSEDYGKHLHGVEDLLQTHTLVEADIAVQAERVKSVTAVAQKFMTEDEGYKPCDPELVRNRVHLLEQRYAELCNLSAARRSKLEESRRLWKFFWDMGEEEAWIREQSRLLLSDDYGKDLTSSLRLISKHNAFRGEMSGHRGPLQQTVAEGEELISKGHFGAGEIEERIKEVKGQWTEMEALAEDRERRLREAYSYYQFQADANDAEAWILDTLRLVSTSEVGHDEYSTQTLVKKHKEIEEEILSHRPAIDALHEQARGLPPAYSHSPEVDGRLPALEQRYEELVALAAHRKQALQDSLALHKMFSEADACLLWIDEKEQWLNVTEIPEKLEDLEVVQQRFETLEAEMNNLASRIASVNEVAGQLLTTDQRNENSIRDMQDQLNSRWNEFRKLVDQKKGALDSALDIQNYHLECNETVCWMREKTKVIESTQSLGNDLAGVTALQRKLTGMERDLKAIQGKVGDLRSEAEKLADGHPQKAPAIIGRLSDIDETWGELKDTMKRREETLGEASKLQGFLRDLDDFQSWLSRAQTAVASEDVPADLAEAERLLAQHEGIKNEIDHYGADYHRVRDVGEEVTRGQTDAQYMFLQQRLKALDTGWNELGQMWENRHQLLSQGHSFQQFLKDTKQVEGFLNNQEYVLSHTEMPSTLQAAEAAIKRHEDFLTTMDANEEKIQGLVESGRKLIEEQNVNSEKIQEKVTSIDERHKKNQKTASEVLTRLKDNRDLQHFLQDCQELTLWINEKMLTAQDVSYDEARNLHSKWQKHQAFMAELASNKDWLDKIDKEGQQLMSEKPELEPVVREKLDTLHKLWNDLESTTQSKAQCLFDANRAELFTQSCSALESWLQNLESQLQSDHYGKDLTSVNILLNKQQMLENQMEVREKELEAIQSQAQALGQEDANIVEVDSKHKDVADKFSKLRAPLNKRRKLLLASKEGHQFNRDLEDEILWVKEKMPMAISTDHGKDLPTVQLLIKKNQTLQKEILGHQPRIDDILERGKSMIQDSSLDPQEIEERLGLLKSTWEELAAEGGKRHARLEESLAAQQFYADAAEAEAWMGEQELHMMSEEKAKDEIGAQAMLKKHLVMEQALDDYADNIHQLANSSRDMVSRGHPESDRITLRQGQVDKLYASLKDLAEERQGKLQEHHRLCQLKRDVEDLEHWISEREVVAASHELGQDYEHVTMLRDKFREFSRDTSNIGQERVDAVNLMADDLISSGHSENATVADWKDQLNEAWADLLEMIDTRSQMLAASYELHRFYQDAKETLGQIQDKQKQIPEETGRDLNTAEAMQRMHTAFEHDIQALSAQVKQVQEDASRLQKAYAGEKAADIHRHEQAVSEAWGELQNASGARRQLLLDTVEKFRFFRMVRDLMLWMDDINLLIDAQEKPRDVSSADLVIQNHLGIKAEVDARADSFTTCIDLGNSLLEKNHYASDKISEKLEQLQERRQETENKWKDKMAWLRVVHEVLMFGRDASVAEAWLASQEPIVRSSELGGNVDEVESLIKRHEAFQKAVSTWDERFVLLEKLTTLEEIEMRRKREEEERKKRPPTPPLPVEEPREEVVKTQVSQTIEGQTDPANILQERINGMQNSFQEQVSVNGLCTESDSPQPSSDTQRLEDSISENQGPEQSDVTNGARTEVITKDGSPTPSPQLVARPTFSPINLQYSTLPSSPTEPLASEQMEGMLHRKQEMDSHNKKAANRSWQNVYCALKRGSLGFYKDAKSAASGVSYHSEPSVQLNGAQCSVAHDYKKRKNVFKLSLLDGKEYLFQAKDEAEMSSWMRVINASLTSSPGDPSDHSPGIMKGMTRAMTMPPVSPNSGTEGSVTLRNKDGKEKDREKRFSFFKKK

Target ORF information:

RefSeq Version	XM_012962405.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis spectrin, beta, non-erythrocytic 2 (sptbn2), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012962405.2

ORF Insert Sequence:

ATGAGCAGTC CACTGTCACC GACAGATTAT GACAGCCTGG AAATCCAGCA GCAGTATAAT 
GACATCAACA ATCGATGGGA TCTGACAGAT GATGATTGGG ACAATGAGAA CAGTTCTGCA 
AGACTTTTTG AGAGGTCCCG GATCAAAGCA CTTGCAGAAA TGGATTTTGT CCCCCGATCT 
GGCTCCCCTC TCCTATCCCC TGCGGCTTTT GTAAATGCAG TGCAATACGC TAACGTGCTG 
GAAGGAAGGT TCAAGCAGCT GCAAGATGAA AGAGAAGCTG TGCAGAAGAA GACATTTACT 
AAATGGGTCA ACTCTCACTT GGCGAGGGTC ACCTGTAGAA TTTCTGATCT CTACAATGAT 
TTACGAGATG GACGGATGTT AATTCGACTG TTAGAAGTGC TATCTGGAGA GCAGCTGCCA 
AAACCCACAA AAGGTCGAAT GCGAATCCAC TGTTTGGAAA ATGTAGACAA AGCCCTTCAA 
TTTTTAAAAG AGCAGAAGGT ACATTTGGAG AACATGGGAT CACATGACAT TGTGGATGGA 
AACCATCGAT TAACCCTAGG GTTAATCTGG ACTATTATTT TAAGGTTTCA GATCCAAGAT 
ATCAGTGTAG AGACAGAAGA TAATAAAGAA AAGAAGTCTG CTAAAGATGC CTTACTGCTT 
TGGTGCCAGA TGAAGACTGC TGGGTATCCA AATGTCAATG TTCACAACTT TACCACCAGC 
TGGCGAGATG GACTTGCGTT TAATGCCATT GTACACAAGC ACAGACCAGA TATAATTGAC 
TTTGACACAC TGAAGAAGTC TAATGCACAC CACAACCTCC AGAATGCCTT CAATGTAGCT 
GAAAGAGAGT TAGGTCTTAC TAAACTTCTG GATCCTGAAG ATGTTAATGT GGATCAGCCT 
GATGAGAAAT CCATCATAAC CTATGTGGCC ACATTTTATC ACTATTTTTC CAAAATGAAA 
GCCCTAGCTG TGGAAGGAAA GCGTATTGGA AAGGTACTTG ACCAAGCTAT TGAAGCTGAT 
CAGTTAATTG AGAACTATGA AGGACTGGCA TCAGAACTCC TGCAATGGAT TGAACAGATG 
ATCATTACTC TCAATGACCG AAAACTGGCA AACTCTCTAA GTGGTGTTCA GAACCAGCTG 
CAGGCCTTCA ACACCTACAG GACTGTAGAA AAGCCGCCCA AGTTCACAGA AAAGGGCAAT 
TTGGAAGTTC TTCTCTTTAC CATACAGAGT AAAATGAGAG CTAATAATCA AAAAGTGTAT 
ACGCCCAGAG AAGGAAAGCT TATATCTGAT ATAAACAAGG CATGGGAGCG ACTAGAAAAG 
GCAGAGCATG AACGTGAGCT TGCCCTTCGC AATGAACTTA TACGCCAGGA AAAATTAGAG 
CAGTTGGCTG CACGATTTGA CCGTAAAGCA GCCATGAGGG AAACCTGGCT AAGTGAAAAT 
CAGAGACTTG TAGCTCAGGA TAACTTCGGT GTGGACTTAA CTGCGGTGGA GGCAGCGCTA 
CGGAAACATG AAGCTATTGA AACTGATATA TTGGCTTACA GTGAGCGCGT GCAAGCAGTG 
AATGCAGTGG CTGATGAGCT GGAAGCAGAG AATTACCATG ATGTCCGGAG GATTTTGGCT 
CGGAAGTGCA ATGTGATTCG TCTCTCTGAT TATTTAAGAG AGCTTGTCAC TGCCAGGAGG 
CAAAGGCTTC ATATGAACCT AGAATTGCAG AGGATGTTCC AGGATTTACA TTATCTCATG 
GACTGGACTG AAGATATGAA GGGCCGTCTG CAGTCAGAAG ATTATGGCAA GCATCTTCAT 
GGCGTGGAAG ACCTTCTCCA GACTCACACT CTTGTGGAGG CAGACATTGC AGTTCAGGCA 
GAACGTGTGA AATCAGTTAC AGCTGTAGCT CAGAAATTTA TGACAGAAGA TGAAGGCTAC 
AAGCCTTGCG ACCCAGAGCT AGTGCGCAAC CGGGTGCATC TCTTGGAACA GCGCTATGCA 
GAGCTGTGTA ATCTTTCTGC GGCTCGGAGA TCAAAGCTGG AAGAATCCCG CCGTCTCTGG 
AAATTTTTCT GGGACATGGG AGAGGAGGAA GCCTGGATCC GTGAGCAGAG TAGATTACTA 
CTTTCTGATG ACTATGGGAA AGACCTTACC AGCTCACTTA GGTTGATCAG CAAGCATAAT 
GCCTTCAGAG GTGAGATGAG TGGACACCGT GGGCCGTTGC AGCAGACTGT GGCTGAGGGT 
GAGGAACTAA TATCCAAAGG TCACTTTGGT GCAGGAGAGA TTGAGGAAAG GATAAAAGAA 
GTGAAAGGCC AGTGGACAGA GATGGAGGCC TTAGCTGAAG ACAGGGAAAG GAGGCTAAGA 
GAGGCCTACA GCTATTATCA GTTTCAAGCT GATGCCAATG ATGCTGAGGC TTGGATCCTG 
GACACTTTAC GCCTTGTTTC TACCAGTGAA GTAGGACATG ATGAGTATTC CACACAGACA 
CTGGTGAAAA AGCACAAAGA AATAGAAGAG GAGATCCTTA GCCACCGACC AGCTATTGAT 
GCGCTGCATG AACAGGCCCG TGGCCTTCCA CCTGCTTACT CTCATTCACC TGAAGTTGAT 
GGAAGGTTAC CAGCCCTGGA GCAGCGCTAT GAGGAATTGG TGGCTCTTGC TGCTCACCGC 
AAGCAGGCTC TGCAAGATTC TCTGGCTCTT CATAAAATGT TTAGTGAAGC TGATGCTTGC 
CTTTTGTGGA TTGATGAGAA AGAACAGTGG CTTAATGTCA CAGAGATTCC AGAAAAACTT 
GAGGACCTAG AAGTAGTTCA ACAAAGGTTT GAAACTCTAG AGGCAGAGAT GAATAATTTG 
GCATCCCGGA TTGCATCTGT AAATGAAGTT GCTGGACAGT TGCTGACTAC AGACCAGAGA 
AATGAAAACA GCATCCGAGA CATGCAAGAT CAACTTAATT CTCGGTGGAA TGAATTCCGC 
AAGCTTGTTG ACCAAAAGAA GGGTGCTTTG GATTCTGCTC TGGATATACA GAATTATCAT 
TTGGAGTGCA ATGAGACTGT GTGCTGGATG AGGGAGAAGA CAAAGGTTAT TGAATCCACA 
CAGAGCCTAG GGAATGATCT TGCAGGAGTA ACAGCCCTCC AGAGAAAACT TACTGGAATG 
GAGCGTGATC TGAAAGCCAT TCAGGGAAAA GTGGGTGACC TGCGTTCAGA GGCAGAAAAG 
CTTGCAGATG GGCACCCACA GAAAGCACCG GCCATAATTG GCCGCTTATC AGATATTGAT 
GAGACCTGGG GAGAACTAAA GGACACTATG AAAAGAAGAG AAGAAACTTT AGGTGAAGCA 
AGCAAGCTGC AGGGATTTTT GAGAGACCTT GATGATTTCC AGAGCTGGTT GTCCCGGGCC 
CAGACAGCAG TGGCATCTGA GGATGTTCCA GCTGACCTAG CAGAAGCAGA GCGCCTTCTT 
GCACAACATG AAGGCATAAA AAATGAGATT GATCATTATG GAGCTGACTA CCACAGAGTG 
AGAGATGTTG GTGAAGAAGT CACTCGTGGG CAAACAGATG CCCAATATAT GTTCTTGCAA 
CAGAGACTGA AGGCGCTAGA CACTGGCTGG AATGAGCTTG GACAGATGTG GGAAAACAGG 
CACCAGTTGT TGTCCCAGGG TCACAGCTTC CAGCAGTTTT TGAAAGATAC AAAACAAGTG 
GAGGGGTTCC TTAATAACCA GGAGTATGTC CTTTCCCATA CCGAAATGCC AAGTACGCTA 
CAGGCAGCTG AAGCTGCCAT CAAACGCCAT GAAGATTTCC TCACAACCAT GGATGCCAAT 
GAAGAGAAGA TACAGGGTCT TGTAGAATCT GGGAGGAAGC TAATTGAGGA ACAGAATGTC 
AATTCTGAGA AGATTCAAGA AAAAGTCACT TCTATTGATG AGAGACACAA AAAGAACCAA 
AAAACTGCTA GCGAAGTATT AACTCGTTTG AAGGACAACC GTGATCTTCA ACATTTCCTT 
CAAGACTGTC AAGAGCTCAC CCTCTGGATC AATGAAAAAA TGCTTACTGC TCAAGATGTT 
TCTTATGATG AAGCTCGAAA CCTGCATAGC AAGTGGCAAA AGCATCAGGC ATTCATGGCA 
GAACTGGCCT CAAATAAAGA CTGGCTTGAC AAAATAGACA AGGAAGGGCA GCAGCTCATG 
TCAGAAAAAC CGGAGCTTGA ACCAGTAGTG AGAGAAAAAC TAGACACTCT GCATAAGCTG 
TGGAATGACT TGGAATCTAC CACACAAAGT AAAGCTCAGT GTCTGTTTGA TGCCAACAGG 
GCAGAGTTAT TCACACAAAG CTGTTCTGCT CTGGAGAGTT GGCTTCAAAA CCTCGAGTCA 
CAACTACAGT CAGATCATTA TGGCAAAGAT CTGACCAGTG TCAACATCCT ATTAAATAAA 
CAGCAGATGC TGGAGAACCA AATGGAAGTG AGGGAGAAGG AGCTAGAAGC TATACAGTCC 
CAGGCACAGG CCCTTGGACA GGAGGATGCC AACATAGTGG AGGTTGACTC AAAGCATAAG 
GATGTAGCAG ATAAATTTTC CAAGCTGCGT GCTCCTCTAA ACAAGAGAAG AAAATTACTT 
CTAGCCTCCA AGGAAGGACA TCAGTTTAAC AGGGACCTGG AAGATGAAAT ACTTTGGGTA 
AAGGAGAAGA TGCCAATGGC AATCTCCACT GATCATGGAA AAGATCTCCC GACAGTCCAG 
CTTCTTATCA AAAAGAATCA GACACTCCAA AAGGAAATTT TGGGACATCA ACCACGGATT 
GATGACATCC TGGAGAGAGG CAAATCTATG ATCCAAGACA GCTCTCTGGA CCCTCAAGAG 
ATAGAAGAAA GGCTTGGGCT ACTGAAAAGC ACATGGGAAG AGCTGGCAGC AGAAGGAGGA 
AAGCGCCATG CCCGTTTAGA GGAATCTCTT GCTGCTCAGC AGTTTTATGC TGATGCGGCA 
GAAGCTGAGG CCTGGATGGG AGAGCAAGAG TTACATATGA TGTCTGAAGA AAAAGCCAAG 
GATGAGATTG GTGCCCAGGC TATGCTGAAG AAACACTTAG TAATGGAACA GGCTCTTGAT 
GACTATGCAG ACAACATCCA TCAGCTTGCC AATTCAAGCC GCGACATGGT CTCCAGAGGT 
CATCCAGAAA GTGATCGTAT TACCCTTCGT CAAGGGCAAG TGGACAAGCT ATATGCCAGT 
CTTAAAGACC TGGCAGAGGA GAGACAAGGA AAGCTACAAG AGCACCACAG ACTTTGCCAA 
CTCAAGAGAG ATGTCGAAGA CCTTGAGCAC TGGATCTCTG AACGTGAGGT GGTTGCAGCT 
TCTCATGAAC TTGGGCAAGA TTATGAGCAT GTAACGATGC TGAGAGACAA ATTCCGTGAG 
TTCTCCCGTG ACACCAGTAA CATTGGTCAA GAGCGGGTGG ATGCTGTCAA TCTGATGGCA 
GATGATTTAA TTTCATCTGG ACATTCGGAA AATGCTACTG TAGCAGATTG GAAAGACCAG 
CTAAATGAGG CATGGGCTGA TCTTCTTGAG ATGATCGACA CTCGAAGTCA GATGCTGGCA 
GCCTCATATG AACTTCACCG TTTCTACCAG GATGCAAAAG AAACCCTAGG TCAGATCCAA 
GACAAGCAGA AACAAATACC AGAGGAGACA GGCAGAGATC TGAATACAGC AGAAGCCATG 
CAGCGCATGC ACACCGCTTT TGAACATGAT ATCCAAGCCC TGAGTGCACA GGTGAAGCAA 
GTCCAGGAAG ATGCTTCTCG CCTCCAAAAA GCTTATGCTG GTGAAAAGGC AGCAGACATT 
CATCGCCATG AGCAGGCAGT ATCGGAAGCT TGGGGAGAGT TGCAGAATGC TAGTGGTGCC 
AGGCGGCAGC TTCTTCTTGA CACTGTGGAG AAATTCCGTT TCTTCAGAAT GGTGCGAGAT 
CTCATGTTAT GGATGGATGA TATAAACCTG CTAATTGATG CACAGGAGAA GCCAAGAGAT 
GTATCCTCAG CTGATTTAGT CATCCAAAAC CACCTAGGCA TCAAGGCAGA AGTGGATGCG 
CGAGCTGACA GCTTTACTAC ATGCATAGAT CTTGGCAACT CTCTCCTAGA GAAGAATCAT 
TATGCCTCTG ACAAGATTTC AGAGAAACTG GAGCAATTGC AGGAAAGAAG GCAGGAGACT 
GAAAACAAAT GGAAAGATAA AATGGCATGG CTGAGAGTTG TCCACGAAGT GCTGATGTTT 
GGAAGAGATG CAAGTGTAGC TGAGGCTTGG CTGGCCAGCC AGGAGCCCAT TGTCCGGTCG 
TCAGAACTTG GTGGCAATGT CGATGAAGTA GAATCCCTGA TAAAAAGACA CGAAGCCTTT 
CAGAAAGCAG TTTCTACATG GGATGAGAGA TTTGTTCTTC TTGAAAAACT AACCACGTTA 
GAAGAAATTG AAATGCGTCG GAAGCGGGAA GAGGAAGAAA GAAAGAAAAG ACCACCAACA 
CCACCTCTAC CAGTAGAAGA GCCTCGAGAG GAAGTTGTAA AAACCCAAGT TTCCCAGACT 
ATTGAAGGAC AGACTGATCC AGCCAATATA CTTCAAGAAA GGATAAATGG CATGCAGAAT 
TCATTCCAGG AGCAAGTATC TGTTAATGGA CTGTGCACAG AGTCTGATTC TCCACAGCCA 
TCGTCCGATA CACAGAGGTT AGAAGACAGC ATCTCTGAAA ATCAGGGTCC GGAACAGTCA 
GATGTAACCA ATGGAGCCAG GACTGAGGTT ATTACCAAAG ATGGAAGTCC AACACCTTCT 
CCACAACTTG TGGCTAGGCC AACCTTCTCC CCGATAAACC TCCAGTATTC TACTTTGCCA 
TCTAGCCCCA CTGAACCATT GGCATCTGAG CAAATGGAAG GAATGCTGCA CCGCAAGCAG 
GAAATGGATT CTCACAACAA GAAGGCTGCG AACAGGTCCT GGCAGAATGT TTACTGTGCA 
CTGAAAAGGG GCAGTCTGGG TTTCTACAAG GATGCTAAAA GTGCAGCCAG TGGCGTTTCT 
TATCATTCGG AGCCCTCCGT CCAACTTAAT GGAGCCCAGT GCAGTGTTGC TCATGACTAC 
AAGAAACGCA AGAATGTCTT CAAGCTAAGC CTTCTTGATG GCAAAGAATA TCTGTTCCAA 
GCTAAGGATG AGGCTGAAAT GAGTAGTTGG ATGCGAGTTA TAAACGCCTC CCTAACTTCC 
TCCCCGGGAG ACCCCTCAGA CCATTCACCG GGCATCATGA AGGGTATGAC CCGGGCCATG 
ACAATGCCAC CAGTATCACC CAACAGTGGT ACAGAGGGAT CAGTCACATT GCGCAACAAG 
GACGGGAAAG AAAAAGACCG GGAAAAGAGA TTCAGTTTCT TTAAGAAGAA GTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

sptbn2 ( XM_002940505.5 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_002940551.2 Xenopus tropicalis spectrin, beta, non-erythrocytic 2 (sptbn2), transcript variant X1, mRNA.

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CloneID	OXa56729
Clone ID Related Accession (Same CDS sequence)	XM_002940505.5
Accession Version	XM_002940505.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7221bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1576512000000
Organism	Xenopus tropicalis(tropical clawed frog)
Product	spectrin beta chain, non-erythrocytic 2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030680.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_002940505.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTTCAGT TCGACTCCCC TGCATACGAG GATGTGCCCC GGCCTGCCTC CCCCGGTCCC 
CGGCTTGCCT CCCCTGTCCC CCGATCTGGC TCCCCTCTCC TATCCCCTGC GGCTTTTGTA 
AATGCAGTGC AATACGCTAA CGTGCTGGAA GGAAGGTTCA AGCAGCTGCA AGATGAAAGA 
GAAGCTGTGC AGAAGAAGAC ATTTACTAAA TGGGTCAACT CTCACTTGGC GAGGGTCACC 
TGTAGAATTT CTGATCTCTA CAATGATTTA CGAGATGGAC GGATGTTAAT TCGACTGTTA 
GAAGTGCTAT CTGGAGAGCA GCTGCCAAAA CCCACAAAAG GTCGAATGCG AATCCACTGT 
TTGGAAAATG TAGACAAAGC CCTTCAATTT TTAAAAGAGC AGAAGGTACA TTTGGAGAAC 
ATGGGATCAC ATGACATTGT GGATGGAAAC CATCGATTAA CCCTAGGGTT AATCTGGACT 
ATTATTTTAA GGTTTCAGAT CCAAGATATC AGTGTAGAGA CAGAAGATAA TAAAGAAAAG 
AAGTCTGCTA AAGATGCCTT ACTGCTTTGG TGCCAGATGA AGACTGCTGG GTATCCAAAT 
GTCAATGTTC ACAACTTTAC CACCAGCTGG CGAGATGGAC TTGCGTTTAA TGCCATTGTA 
CACAAGCACA GACCAGATAT AATTGACTTT GACACACTGA AGAAGTCTAA TGCACACCAC 
AACCTCCAGA ATGCCTTCAA TGTAGCTGAA AGAGAGTTAG GTCTTACTAA ACTTCTGGAT 
CCTGAAGATG TTAATGTGGA TCAGCCTGAT GAGAAATCCA TCATAACCTA TGTGGCCACA 
TTTTATCACT ATTTTTCCAA AATGAAAGCC CTAGCTGTGG AAGGAAAGCG TATTGGAAAG 
GTACTTGACC AAGCTATTGA AGCTGATCAG TTAATTGAGA ACTATGAAGG ACTGGCATCA 
GAACTCCTGC AATGGATTGA ACAGATGATC ATTACTCTCA ATGACCGAAA ACTGGCAAAC 
TCTCTAAGTG GTGTTCAGAA CCAGCTGCAG GCCTTCAACA CCTACAGGAC TGTAGAAAAG 
CCGCCCAAGT TCACAGAAAA GGGCAATTTG GAAGTTCTTC TCTTTACCAT ACAGAGTAAA 
ATGAGAGCTA ATAATCAAAA AGTGTATACG CCCAGAGAAG GAAAGCTTAT ATCTGATATA 
AACAAGGCAT GGGAGCGACT AGAAAAGGCA GAGCATGAAC GTGAGCTTGC CCTTCGCAAT 
GAACTTATAC GCCAGGAAAA ATTAGAGCAG TTGGCTGCAC GATTTGACCG TAAAGCAGCC 
ATGAGGGAAA CCTGGCTAAG TGAAAATCAG AGACTTGTAG CTCAGGATAA CTTCGGTGTG 
GACTTAACTG CGGTGGAGGC AGCGCTACGG AAACATGAAG CTATTGAAAC TGATATATTG 
GCTTACAGTG AGCGCGTGCA AGCAGTGAAT GCAGTGGCTG ATGAGCTGGA AGCAGAGAAT 
TACCATGATG TCCGGAGGAT TTTGGCTCGG AAGTGCAATG TGATTCGTCT CTCTGATTAT 
TTAAGAGAGC TTGTCACTGC CAGGAGGCAA AGGCTTCATA TGAACCTAGA ATTGCAGAGG 
ATGTTCCAGG ATTTACATTA TCTCATGGAC TGGACTGAAG ATATGAAGGG CCGTCTGCAG 
TCAGAAGATT ATGGCAAGCA TCTTCATGGC GTGGAAGACC TTCTCCAGAC TCACACTCTT 
GTGGAGGCAG ACATTGCAGT TCAGGCAGAA CGTGTGAAAT CAGTTACAGC TGTAGCTCAG 
AAATTTATGA CAGAAGATGA AGGCTACAAG CCTTGCGACC CAGAGCTAGT GCGCAACCGG 
GTGCATCTCT TGGAACAGCG CTATGCAGAG CTGTGTAATC TTTCTGCGGC TCGGAGATCA 
AAGCTGGAAG AATCCCGCCG TCTCTGGAAA TTTTTCTGGG ACATGGGAGA GGAGGAAGCC 
TGGATCCGTG AGCAGAGTAG ATTACTACTT TCTGATGACT ATGGGAAAGA CCTTACCAGC 
TCACTTAGGT TGATCAGCAA GCATAATGCC TTCAGAGGTG AGATGAGTGG ACACCGTGGG 
CCGTTGCAGC AGACTGTGGC TGAGGGTGAG GAACTAATAT CCAAAGGTCA CTTTGGTGCA 
GGAGAGATTG AGGAAAGGAT AAAAGAAGTG AAAGGCCAGT GGACAGAGAT GGAGGCCTTA 
GCTGAAGACA GGGAAAGGAG GCTAAGAGAG GCCTACAGCT ATTATCAGTT TCAAGCTGAT 
GCCAATGATG CTGAGGCTTG GATCCTGGAC ACTTTACGCC TTGTTTCTAC CAGTGAAGTA 
GGACATGATG AGTATTCCAC ACAGACACTG GTGAAAAAGC ACAAAGAAAT AGAAGAGGAG 
ATCCTTAGCC ACCGACCAGC TATTGATGCG CTGCATGAAC AGGCCCGTGG CCTTCCACCT 
GCTTACTCTC ATTCACCTGA AGTTGATGGA AGGTTACCAG CCCTGGAGCA GCGCTATGAG 
GAATTGGTGG CTCTTGCTGC TCACCGCAAG CAGGCTCTGC AAGATTCTCT GGCTCTTCAT 
AAAATGTTTA GTGAAGCTGA TGCTTGCCTT TTGTGGATTG ATGAGAAAGA ACAGTGGCTT 
AATGTCACAG AGATTCCAGA AAAACTTGAG GACCTAGAAG TAGTTCAACA AAGGTTTGAA 
ACTCTAGAGG CAGAGATGAA TAATTTGGCA TCCCGGATTG CATCTGTAAA TGAAGTTGCT 
GGACAGTTGC TGACTACAGA CCAGAGAAAT GAAAACAGCA TCCGAGACAT GCAAGATCAA 
CTTAATTCTC GGTGGAATGA ATTCCGCAAG CTTGTTGACC AAAAGAAGGG TGCTTTGGAT 
TCTGCTCTGG ATATACAGAA TTATCATTTG GAGTGCAATG AGACTGTGTG CTGGATGAGG 
GAGAAGACAA AGGTTATTGA ATCCACACAG AGCCTAGGGA ATGATCTTGC AGGAGTAACA 
GCCCTCCAGA GAAAACTTAC TGGAATGGAG CGTGATCTGA AAGCCATTCA GGGAAAAGTG 
GGTGACCTGC GTTCAGAGGC AGAAAAGCTT GCAGATGGGC ACCCACAGAA AGCACCGGCC 
ATAATTGGCC GCTTATCAGA TATTGATGAG ACCTGGGGAG AACTAAAGGA CACTATGAAA 
AGAAGAGAAG AAACTTTAGG TGAAGCAAGC AAGCTGCAGG GATTTTTGAG AGACCTTGAT 
GATTTCCAGA GCTGGTTGTC CCGGGCCCAG ACAGCAGTGG CATCTGAGGA TGTTCCAGCT 
GACCTAGCAG AAGCAGAGCG CCTTCTTGCA CAACATGAAG GCATAAAAAA TGAGATTGAT 
CATTATGGAG CTGACTACCA CAGAGTGAGA GATGTTGGTG AAGAAGTCAC TCGTGGGCAA 
ACAGATGCCC AATATATGTT CTTGCAACAG AGACTGAAGG CGCTAGACAC TGGCTGGAAT 
GAGCTTGGAC AGATGTGGGA AAACAGGCAC CAGTTGTTGT CCCAGGGTCA CAGCTTCCAG 
CAGTTTTTGA AAGATACAAA ACAAGTGGAG GGGTTCCTTA ATAACCAGGA GTATGTCCTT 
TCCCATACCG AAATGCCAAG TACGCTACAG GCAGCTGAAG CTGCCATCAA ACGCCATGAA 
GATTTCCTCA CAACCATGGA TGCCAATGAA GAGAAGATAC AGGGTCTTGT AGAATCTGGG 
AGGAAGCTAA TTGAGGAACA GAATGTCAAT TCTGAGAAGA TTCAAGAAAA AGTCACTTCT 
ATTGATGAGA GACACAAAAA GAACCAAAAA ACTGCTAGCG AAGTATTAAC TCGTTTGAAG 
GACAACCGTG ATCTTCAACA TTTCCTTCAA GACTGTCAAG AGCTCACCCT CTGGATCAAT 
GAAAAAATGC TTACTGCTCA AGATGTTTCT TATGATGAAG CTCGAAACCT GCATAGCAAG 
TGGCAAAAGC ATCAGGCATT CATGGCAGAA CTGGCCTCAA ATAAAGACTG GCTTGACAAA 
ATAGACAAGG AAGGGCAGCA GCTCATGTCA GAAAAACCGG AGCTTGAACC AGTAGTGAGA 
GAAAAACTAG ACACTCTGCA TAAGCTGTGG AATGACTTGG AATCTACCAC ACAAAGTAAA 
GCTCAGTGTC TGTTTGATGC CAACAGGGCA GAGTTATTCA CACAAAGCTG TTCTGCTCTG 
GAGAGTTGGC TTCAAAACCT CGAGTCACAA CTACAGTCAG ATCATTATGG CAAAGATCTG 
ACCAGTGTCA ACATCCTATT AAATAAACAG CAGATGCTGG AGAACCAAAT GGAAGTGAGG 
GAGAAGGAGC TAGAAGCTAT ACAGTCCCAG GCACAGGCCC TTGGACAGGA GGATGCCAAC 
ATAGTGGAGG TTGACTCAAA GCATAAGGAT GTAGCAGATA AATTTTCCAA GCTGCGTGCT 
CCTCTAAACA AGAGAAGAAA ATTACTTCTA GCCTCCAAGG AAGGACATCA GTTTAACAGG 
GACCTGGAAG ATGAAATACT TTGGGTAAAG GAGAAGATGC CAATGGCAAT CTCCACTGAT 
CATGGAAAAG ATCTCCCGAC AGTCCAGCTT CTTATCAAAA AGAATCAGAC ACTCCAAAAG 
GAAATTTTGG GACATCAACC ACGGATTGAT GACATCCTGG AGAGAGGCAA ATCTATGATC 
CAAGACAGCT CTCTGGACCC TCAAGAGATA GAAGAAAGGC TTGGGCTACT GAAAAGCACA 
TGGGAAGAGC TGGCAGCAGA AGGAGGAAAG CGCCATGCCC GTTTAGAGGA ATCTCTTGCT 
GCTCAGCAGT TTTATGCTGA TGCGGCAGAA GCTGAGGCCT GGATGGGAGA GCAAGAGTTA 
CATATGATGT CTGAAGAAAA AGCCAAGGAT GAGATTGGTG CCCAGGCAAT GCTGAAGAAA 
CACTTAGTAA TGGAACAGGC TCTTGATGAC TATGCAGACA ACATCCATCA GCTTGCCAAT 
TCAAGCCGCG ACATGGTCTC CAGAGGTCAT CCAGAAAGTG ATCGTATTAC CCTTCGTCAA 
GGGCAAGTGG ACAAGCTATA TGCCAGTCTT AAAGACCTGG CAGAGGAGAG ACAAGGAAAG 
CTACAAGAGC ACCACAGACT TTGCCAACTC AAGAGAGATG TCGAAGACCT TGAGCACTGG 
ATCTCTGAAC GTGAGGTGGT TGCAGCTTCT CATGAACTTG GGCAAGATTA TGAGCATGTA 
ACGATGCTGA GAGACAAATT CCGTGAGTTC TCCCGTGACA CCAGTAACAT TGGTCAAGAG 
CGGGTGGATG CTGTCAATCT GATGGCAGAT GATTTAATTT CATCTGGACA TTCGGAAAAT 
GCTACTGTAG CAGATTGGAA AGACCAGCTA AATGAGGCAT GGGCTGATCT TCTTGAGATG 
ATTGACACTC GAAGTCAGAT GCTGGCAGCC TCATATGAAC TTCACCGTTT CTACCAGGAT 
GCAAAAGAAA CCCTAGGTCA GATCCAAGAC AAGCAGAAAC AAATACCAGA GGAGACAGGC 
AGAGATCTGA ATACAGCAGA AGCCATGCAG CGCATGCACA CCGCTTTTGA ACATGATATC 
CAAGCCCTGA GTGCACAGGT GAAGCAAGTC CAGGAAGATG CTTCTCGCCT CCAAAAAGCT 
TATGCTGGTG AAAAGGCAGC AGACATTCAT CGCCATGAGC AGGCAGTATC GGAAGCTTGG 
GGAGAGTTGC AGAATGCTAG TGGTGCCAGG CGGCAGCTTC TTCTTGACAC TGTGGAGAAA 
TTCCGTTTCT TCAGAATGGT GCGAGATCTC ATGTTATGGA TGGATGATAT AAACCTGCTA 
ATTGATGCAC AGGAGAAGCC AAGAGATGTA TCCTCAGCTG ATTTAGTCAT CCAAAACCAC 
CTAGGCATCA AGGCAGAAGT GGATGCGCGA GCTGACAGCT TTACTACATG CATAGATCTT 
GGCAACTCTC TCCTAGAGAA GAATCATTAT GCCTCTGACA AGATTTCAGA GAAACTGGAG 
CAATTGCAGG AAAGAAGGCA GGAGACTGAA AACAAATGGA AAGATAAAAT GGCATGGCTG 
AGAGTTGTCC ACGAAGTGCT GATGTTTGGA AGAGATGCAA GTGTAGCTGA GGCTTGGCTG 
GCCAGCCAGG AGCCCATTGT CCGGTCGTCA GAACTTGGTG GCAATGTCGA TGAAGTAGAA 
TCCCTGATAA AAAGACACGA AGCCTTTCAG AAAGCAGTTT CTACATGGGA TGAGAGATTT 
GTTCTTCTTG AAAAACTAAC CACGTTAGAA GAAATTGAAA TGCGTCGGAA GCGGGAAGAG 
GAAGAAAGAA AGAAAAGACC ACCAACACCA CCTCTACCAG TAGAAGAGCC TCGAGAGGAA 
GTTGTAAAAA CCCAAGTTTC CCAGACTATT GAAGGGCAGA CTGATCCAGC CAATATACTT 
CAAGAAAGGA TAAATGGCAT GCAGAATTCA TTCCAGGAGC AAGTATCTGT TAATGGACTG 
TGCACAGAGT CTGATTCTCC ACAGCCATCG TCCGATACAC AGAGGTTAGA AGACAGCATC 
TCTGAAAATC AGGGTCCGGA ACAGTCAGAT GTAACCAATG GAGCCAGGAC TGAGGTTATT 
ACCAAAGATG GAAGTCCAAC ACCTTCTCCA CAACTTGTGG CTAGGCCAAC CTTCTCCCCG 
ATAAACCTCC AGTATTCTAC TTTGCCATCT AGCCCCACTG AACCATTGGC ATCTGAGCAA 
ATGGAAGGAA TGCTGCACCG CAAGCAGGAA ATGGATTCTC ACAACAAGAA GGCTGCGAAC 
AGGTCCTGGC AGAATGTTTA CTGTGCACTG AAAAGGGGCA GTCTGGGTTT CTACAAGGAT 
GCTAAAAGTG CAGCCAGTGG CGTTTCTTAT CATTCGGAGC CCTCCGTCCA ACTTAATGGA 
GCCCAGTGCA GTGTTGCTCA TGACTACAAG AAACGCAAGA ATGTCTTCAA GCTAAGCCTT 
CTTGATGGCA AAGAATATCT GTTCCAAGCT AAGGATGAGG CTGAAATGAG TAGTTGGATG 
CGAGTTATAA ACGCCTCCCT AACTTCCTCC CCGGGAGACC CCTCAGACCA TTCACCGGGC 
ATCATGAAGG GTATGACCCG GGCCATGACA ATGCCACCAG TATCACCCAA CAGTGGTACA 
GAGGGATCAG TCACATTGCG CAACAAGGAC GGGAAAGAAA AAGACCGGGA AAAGAGATTC 
AGTTTCTTTA AGAAGAAGTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_002940551.2
CDS	909..8129
Translation	MFQFDSPAYEDVPRPASPGPRLASPVPRSGSPLLSPAAFVNAVQYANVLEGRFKQLQDEREAVQKKTFTKWVNSHLARVTCRISDLYNDLRDGRMLIRLLEVLSGEQLPKPTKGRMRIHCLENVDKALQFLKEQKVHLENMGSHDIVDGNHRLTLGLIWTIILRFQIQDISVETEDNKEKKSAKDALLLWCQMKTAGYPNVNVHNFTTSWRDGLAFNAIVHKHRPDIIDFDTLKKSNAHHNLQNAFNVAERELGLTKLLDPEDVNVDQPDEKSIITYVATFYHYFSKMKALAVEGKRIGKVLDQAIEADQLIENYEGLASELLQWIEQMIITLNDRKLANSLSGVQNQLQAFNTYRTVEKPPKFTEKGNLEVLLFTIQSKMRANNQKVYTPREGKLISDINKAWERLEKAEHERELALRNELIRQEKLEQLAARFDRKAAMRETWLSENQRLVAQDNFGVDLTAVEAALRKHEAIETDILAYSERVQAVNAVADELEAENYHDVRRILARKCNVIRLSDYLRELVTARRQRLHMNLELQRMFQDLHYLMDWTEDMKGRLQSEDYGKHLHGVEDLLQTHTLVEADIAVQAERVKSVTAVAQKFMTEDEGYKPCDPELVRNRVHLLEQRYAELCNLSAARRSKLEESRRLWKFFWDMGEEEAWIREQSRLLLSDDYGKDLTSSLRLISKHNAFRGEMSGHRGPLQQTVAEGEELISKGHFGAGEIEERIKEVKGQWTEMEALAEDRERRLREAYSYYQFQADANDAEAWILDTLRLVSTSEVGHDEYSTQTLVKKHKEIEEEILSHRPAIDALHEQARGLPPAYSHSPEVDGRLPALEQRYEELVALAAHRKQALQDSLALHKMFSEADACLLWIDEKEQWLNVTEIPEKLEDLEVVQQRFETLEAEMNNLASRIASVNEVAGQLLTTDQRNENSIRDMQDQLNSRWNEFRKLVDQKKGALDSALDIQNYHLECNETVCWMREKTKVIESTQSLGNDLAGVTALQRKLTGMERDLKAIQGKVGDLRSEAEKLADGHPQKAPAIIGRLSDIDETWGELKDTMKRREETLGEASKLQGFLRDLDDFQSWLSRAQTAVASEDVPADLAEAERLLAQHEGIKNEIDHYGADYHRVRDVGEEVTRGQTDAQYMFLQQRLKALDTGWNELGQMWENRHQLLSQGHSFQQFLKDTKQVEGFLNNQEYVLSHTEMPSTLQAAEAAIKRHEDFLTTMDANEEKIQGLVESGRKLIEEQNVNSEKIQEKVTSIDERHKKNQKTASEVLTRLKDNRDLQHFLQDCQELTLWINEKMLTAQDVSYDEARNLHSKWQKHQAFMAELASNKDWLDKIDKEGQQLMSEKPELEPVVREKLDTLHKLWNDLESTTQSKAQCLFDANRAELFTQSCSALESWLQNLESQLQSDHYGKDLTSVNILLNKQQMLENQMEVREKELEAIQSQAQALGQEDANIVEVDSKHKDVADKFSKLRAPLNKRRKLLLASKEGHQFNRDLEDEILWVKEKMPMAISTDHGKDLPTVQLLIKKNQTLQKEILGHQPRIDDILERGKSMIQDSSLDPQEIEERLGLLKSTWEELAAEGGKRHARLEESLAAQQFYADAAEAEAWMGEQELHMMSEEKAKDEIGAQAMLKKHLVMEQALDDYADNIHQLANSSRDMVSRGHPESDRITLRQGQVDKLYASLKDLAEERQGKLQEHHRLCQLKRDVEDLEHWISEREVVAASHELGQDYEHVTMLRDKFREFSRDTSNIGQERVDAVNLMADDLISSGHSENATVADWKDQLNEAWADLLEMIDTRSQMLAASYELHRFYQDAKETLGQIQDKQKQIPEETGRDLNTAEAMQRMHTAFEHDIQALSAQVKQVQEDASRLQKAYAGEKAADIHRHEQAVSEAWGELQNASGARRQLLLDTVEKFRFFRMVRDLMLWMDDINLLIDAQEKPRDVSSADLVIQNHLGIKAEVDARADSFTTCIDLGNSLLEKNHYASDKISEKLEQLQERRQETENKWKDKMAWLRVVHEVLMFGRDASVAEAWLASQEPIVRSSELGGNVDEVESLIKRHEAFQKAVSTWDERFVLLEKLTTLEEIEMRRKREEEERKKRPPTPPLPVEEPREEVVKTQVSQTIEGQTDPANILQERINGMQNSFQEQVSVNGLCTESDSPQPSSDTQRLEDSISENQGPEQSDVTNGARTEVITKDGSPTPSPQLVARPTFSPINLQYSTLPSSPTEPLASEQMEGMLHRKQEMDSHNKKAANRSWQNVYCALKRGSLGFYKDAKSAASGVSYHSEPSVQLNGAQCSVAHDYKKRKNVFKLSLLDGKEYLFQAKDEAEMSSWMRVINASLTSSPGDPSDHSPGIMKGMTRAMTMPPVSPNSGTEGSVTLRNKDGKEKDREKRFSFFKKK

Target ORF information:

RefSeq Version	XM_002940505.5
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis spectrin, beta, non-erythrocytic 2 (sptbn2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_002940505.5

ORF Insert Sequence:

ATGTTTCAGT TCGACTCCCC TGCATACGAG GATGTGCCCC GGCCTGCCTC CCCCGGTCCC 
CGGCTTGCCT CCCCTGTCCC CCGATCTGGC TCCCCTCTCC TATCCCCTGC GGCTTTTGTA 
AATGCAGTGC AATACGCTAA CGTGCTGGAA GGAAGGTTCA AGCAGCTGCA AGATGAAAGA 
GAAGCTGTGC AGAAGAAGAC ATTTACTAAA TGGGTCAACT CTCACTTGGC GAGGGTCACC 
TGTAGAATTT CTGATCTCTA CAATGATTTA CGAGATGGAC GGATGTTAAT TCGACTGTTA 
GAAGTGCTAT CTGGAGAGCA GCTGCCAAAA CCCACAAAAG GTCGAATGCG AATCCACTGT 
TTGGAAAATG TAGACAAAGC CCTTCAATTT TTAAAAGAGC AGAAGGTACA TTTGGAGAAC 
ATGGGATCAC ATGACATTGT GGATGGAAAC CATCGATTAA CCCTAGGGTT AATCTGGACT 
ATTATTTTAA GGTTTCAGAT CCAAGATATC AGTGTAGAGA CAGAAGATAA TAAAGAAAAG 
AAGTCTGCTA AAGATGCCTT ACTGCTTTGG TGCCAGATGA AGACTGCTGG GTATCCAAAT 
GTCAATGTTC ACAACTTTAC CACCAGCTGG CGAGATGGAC TTGCGTTTAA TGCCATTGTA 
CACAAGCACA GACCAGATAT AATTGACTTT GACACACTGA AGAAGTCTAA TGCACACCAC 
AACCTCCAGA ATGCCTTCAA TGTAGCTGAA AGAGAGTTAG GTCTTACTAA ACTTCTGGAT 
CCTGAAGATG TTAATGTGGA TCAGCCTGAT GAGAAATCCA TCATAACCTA TGTGGCCACA 
TTTTATCACT ATTTTTCCAA AATGAAAGCC CTAGCTGTGG AAGGAAAGCG TATTGGAAAG 
GTACTTGACC AAGCTATTGA AGCTGATCAG TTAATTGAGA ACTATGAAGG ACTGGCATCA 
GAACTCCTGC AATGGATTGA ACAGATGATC ATTACTCTCA ATGACCGAAA ACTGGCAAAC 
TCTCTAAGTG GTGTTCAGAA CCAGCTGCAG GCCTTCAACA CCTACAGGAC TGTAGAAAAG 
CCGCCCAAGT TCACAGAAAA GGGCAATTTG GAAGTTCTTC TCTTTACCAT ACAGAGTAAA 
ATGAGAGCTA ATAATCAAAA AGTGTATACG CCCAGAGAAG GAAAGCTTAT ATCTGATATA 
AACAAGGCAT GGGAGCGACT AGAAAAGGCA GAGCATGAAC GTGAGCTTGC CCTTCGCAAT 
GAACTTATAC GCCAGGAAAA ATTAGAGCAG TTGGCTGCAC GATTTGACCG TAAAGCAGCC 
ATGAGGGAAA CCTGGCTAAG TGAAAATCAG AGACTTGTAG CTCAGGATAA CTTCGGTGTG 
GACTTAACTG CGGTGGAGGC AGCGCTACGG AAACATGAAG CTATTGAAAC TGATATATTG 
GCTTACAGTG AGCGCGTGCA AGCAGTGAAT GCAGTGGCTG ATGAGCTGGA AGCAGAGAAT 
TACCATGATG TCCGGAGGAT TTTGGCTCGG AAGTGCAATG TGATTCGTCT CTCTGATTAT 
TTAAGAGAGC TTGTCACTGC CAGGAGGCAA AGGCTTCATA TGAACCTAGA ATTGCAGAGG 
ATGTTCCAGG ATTTACATTA TCTCATGGAC TGGACTGAAG ATATGAAGGG CCGTCTGCAG 
TCAGAAGATT ATGGCAAGCA TCTTCATGGC GTGGAAGACC TTCTCCAGAC TCACACTCTT 
GTGGAGGCAG ACATTGCAGT TCAGGCAGAA CGTGTGAAAT CAGTTACAGC TGTAGCTCAG 
AAATTTATGA CAGAAGATGA AGGCTACAAG CCTTGCGACC CAGAGCTAGT GCGCAACCGG 
GTGCATCTCT TGGAACAGCG CTATGCAGAG CTGTGTAATC TTTCTGCGGC TCGGAGATCA 
AAGCTGGAAG AATCCCGCCG TCTCTGGAAA TTTTTCTGGG ACATGGGAGA GGAGGAAGCC 
TGGATCCGTG AGCAGAGTAG ATTACTACTT TCTGATGACT ATGGGAAAGA CCTTACCAGC 
TCACTTAGGT TGATCAGCAA GCATAATGCC TTCAGAGGTG AGATGAGTGG ACACCGTGGG 
CCGTTGCAGC AGACTGTGGC TGAGGGTGAG GAACTAATAT CCAAAGGTCA CTTTGGTGCA 
GGAGAGATTG AGGAAAGGAT AAAAGAAGTG AAAGGCCAGT GGACAGAGAT GGAGGCCTTA 
GCTGAAGACA GGGAAAGGAG GCTAAGAGAG GCCTACAGCT ATTATCAGTT TCAAGCTGAT 
GCCAATGATG CTGAGGCTTG GATCCTGGAC ACTTTACGCC TTGTTTCTAC CAGTGAAGTA 
GGACATGATG AGTATTCCAC ACAGACACTG GTGAAAAAGC ACAAAGAAAT AGAAGAGGAG 
ATCCTTAGCC ACCGACCAGC TATTGATGCG CTGCATGAAC AGGCCCGTGG CCTTCCACCT 
GCTTACTCTC ATTCACCTGA AGTTGATGGA AGGTTACCAG CCCTGGAGCA GCGCTATGAG 
GAATTGGTGG CTCTTGCTGC TCACCGCAAG CAGGCTCTGC AAGATTCTCT GGCTCTTCAT 
AAAATGTTTA GTGAAGCTGA TGCTTGCCTT TTGTGGATTG ATGAGAAAGA ACAGTGGCTT 
AATGTCACAG AGATTCCAGA AAAACTTGAG GACCTAGAAG TAGTTCAACA AAGGTTTGAA 
ACTCTAGAGG CAGAGATGAA TAATTTGGCA TCCCGGATTG CATCTGTAAA TGAAGTTGCT 
GGACAGTTGC TGACTACAGA CCAGAGAAAT GAAAACAGCA TCCGAGACAT GCAAGATCAA 
CTTAATTCTC GGTGGAATGA ATTCCGCAAG CTTGTTGACC AAAAGAAGGG TGCTTTGGAT 
TCTGCTCTGG ATATACAGAA TTATCATTTG GAGTGCAATG AGACTGTGTG CTGGATGAGG 
GAGAAGACAA AGGTTATTGA ATCCACACAG AGCCTAGGGA ATGATCTTGC AGGAGTAACA 
GCCCTCCAGA GAAAACTTAC TGGAATGGAG CGTGATCTGA AAGCCATTCA GGGAAAAGTG 
GGTGACCTGC GTTCAGAGGC AGAAAAGCTT GCAGATGGGC ACCCACAGAA AGCACCGGCC 
ATAATTGGCC GCTTATCAGA TATTGATGAG ACCTGGGGAG AACTAAAGGA CACTATGAAA 
AGAAGAGAAG AAACTTTAGG TGAAGCAAGC AAGCTGCAGG GATTTTTGAG AGACCTTGAT 
GATTTCCAGA GCTGGTTGTC CCGGGCCCAG ACAGCAGTGG CATCTGAGGA TGTTCCAGCT 
GACCTAGCAG AAGCAGAGCG CCTTCTTGCA CAACATGAAG GCATAAAAAA TGAGATTGAT 
CATTATGGAG CTGACTACCA CAGAGTGAGA GATGTTGGTG AAGAAGTCAC TCGTGGGCAA 
ACAGATGCCC AATATATGTT CTTGCAACAG AGACTGAAGG CGCTAGACAC TGGCTGGAAT 
GAGCTTGGAC AGATGTGGGA AAACAGGCAC CAGTTGTTGT CCCAGGGTCA CAGCTTCCAG 
CAGTTTTTGA AAGATACAAA ACAAGTGGAG GGGTTCCTTA ATAACCAGGA GTATGTCCTT 
TCCCATACCG AAATGCCAAG TACGCTACAG GCAGCTGAAG CTGCCATCAA ACGCCATGAA 
GATTTCCTCA CAACCATGGA TGCCAATGAA GAGAAGATAC AGGGTCTTGT AGAATCTGGG 
AGGAAGCTAA TTGAGGAACA GAATGTCAAT TCTGAGAAGA TTCAAGAAAA AGTCACTTCT 
ATTGATGAGA GACACAAAAA GAACCAAAAA ACTGCTAGCG AAGTATTAAC TCGTTTGAAG 
GACAACCGTG ATCTTCAACA TTTCCTTCAA GACTGTCAAG AGCTCACCCT CTGGATCAAT 
GAAAAAATGC TTACTGCTCA AGATGTTTCT TATGATGAAG CTCGAAACCT GCATAGCAAG 
TGGCAAAAGC ATCAGGCATT CATGGCAGAA CTGGCCTCAA ATAAAGACTG GCTTGACAAA 
ATAGACAAGG AAGGGCAGCA GCTCATGTCA GAAAAACCGG AGCTTGAACC AGTAGTGAGA 
GAAAAACTAG ACACTCTGCA TAAGCTGTGG AATGACTTGG AATCTACCAC ACAAAGTAAA 
GCTCAGTGTC TGTTTGATGC CAACAGGGCA GAGTTATTCA CACAAAGCTG TTCTGCTCTG 
GAGAGTTGGC TTCAAAACCT CGAGTCACAA CTACAGTCAG ATCATTATGG CAAAGATCTG 
ACCAGTGTCA ACATCCTATT AAATAAACAG CAGATGCTGG AGAACCAAAT GGAAGTGAGG 
GAGAAGGAGC TAGAAGCTAT ACAGTCCCAG GCACAGGCCC TTGGACAGGA GGATGCCAAC 
ATAGTGGAGG TTGACTCAAA GCATAAGGAT GTAGCAGATA AATTTTCCAA GCTGCGTGCT 
CCTCTAAACA AGAGAAGAAA ATTACTTCTA GCCTCCAAGG AAGGACATCA GTTTAACAGG 
GACCTGGAAG ATGAAATACT TTGGGTAAAG GAGAAGATGC CAATGGCAAT CTCCACTGAT 
CATGGAAAAG ATCTCCCGAC AGTCCAGCTT CTTATCAAAA AGAATCAGAC ACTCCAAAAG 
GAAATTTTGG GACATCAACC ACGGATTGAT GACATCCTGG AGAGAGGCAA ATCTATGATC 
CAAGACAGCT CTCTGGACCC TCAAGAGATA GAAGAAAGGC TTGGGCTACT GAAAAGCACA 
TGGGAAGAGC TGGCAGCAGA AGGAGGAAAG CGCCATGCCC GTTTAGAGGA ATCTCTTGCT 
GCTCAGCAGT TTTATGCTGA TGCGGCAGAA GCTGAGGCCT GGATGGGAGA GCAAGAGTTA 
CATATGATGT CTGAAGAAAA AGCCAAGGAT GAGATTGGTG CCCAGGCAAT GCTGAAGAAA 
CACTTAGTAA TGGAACAGGC TCTTGATGAC TATGCAGACA ACATCCATCA GCTTGCCAAT 
TCAAGCCGCG ACATGGTCTC CAGAGGTCAT CCAGAAAGTG ATCGTATTAC CCTTCGTCAA 
GGGCAAGTGG ACAAGCTATA TGCCAGTCTT AAAGACCTGG CAGAGGAGAG ACAAGGAAAG 
CTACAAGAGC ACCACAGACT TTGCCAACTC AAGAGAGATG TCGAAGACCT TGAGCACTGG 
ATCTCTGAAC GTGAGGTGGT TGCAGCTTCT CATGAACTTG GGCAAGATTA TGAGCATGTA 
ACGATGCTGA GAGACAAATT CCGTGAGTTC TCCCGTGACA CCAGTAACAT TGGTCAAGAG 
CGGGTGGATG CTGTCAATCT GATGGCAGAT GATTTAATTT CATCTGGACA TTCGGAAAAT 
GCTACTGTAG CAGATTGGAA AGACCAGCTA AATGAGGCAT GGGCTGATCT TCTTGAGATG 
ATTGACACTC GAAGTCAGAT GCTGGCAGCC TCATATGAAC TTCACCGTTT CTACCAGGAT 
GCAAAAGAAA CCCTAGGTCA GATCCAAGAC AAGCAGAAAC AAATACCAGA GGAGACAGGC 
AGAGATCTGA ATACAGCAGA AGCCATGCAG CGCATGCACA CCGCTTTTGA ACATGATATC 
CAAGCCCTGA GTGCACAGGT GAAGCAAGTC CAGGAAGATG CTTCTCGCCT CCAAAAAGCT 
TATGCTGGTG AAAAGGCAGC AGACATTCAT CGCCATGAGC AGGCAGTATC GGAAGCTTGG 
GGAGAGTTGC AGAATGCTAG TGGTGCCAGG CGGCAGCTTC TTCTTGACAC TGTGGAGAAA 
TTCCGTTTCT TCAGAATGGT GCGAGATCTC ATGTTATGGA TGGATGATAT AAACCTGCTA 
ATTGATGCAC AGGAGAAGCC AAGAGATGTA TCCTCAGCTG ATTTAGTCAT CCAAAACCAC 
CTAGGCATCA AGGCAGAAGT GGATGCGCGA GCTGACAGCT TTACTACATG CATAGATCTT 
GGCAACTCTC TCCTAGAGAA GAATCATTAT GCCTCTGACA AGATTTCAGA GAAACTGGAG 
CAATTGCAGG AAAGAAGGCA GGAGACTGAA AACAAATGGA AAGATAAAAT GGCATGGCTG 
AGAGTTGTCC ACGAAGTGCT GATGTTTGGA AGAGATGCAA GTGTAGCTGA GGCTTGGCTG 
GCCAGCCAGG AGCCCATTGT CCGGTCGTCA GAACTTGGTG GCAATGTCGA TGAAGTAGAA 
TCCCTGATAA AAAGACACGA AGCCTTTCAG AAAGCAGTTT CTACATGGGA TGAGAGATTT 
GTTCTTCTTG AAAAACTAAC CACGTTAGAA GAAATTGAAA TGCGTCGGAA GCGGGAAGAG 
GAAGAAAGAA AGAAAAGACC ACCAACACCA CCTCTACCAG TAGAAGAGCC TCGAGAGGAA 
GTTGTAAAAA CCCAAGTTTC CCAGACTATT GAAGGGCAGA CTGATCCAGC CAATATACTT 
CAAGAAAGGA TAAATGGCAT GCAGAATTCA TTCCAGGAGC AAGTATCTGT TAATGGACTG 
TGCACAGAGT CTGATTCTCC ACAGCCATCG TCCGATACAC AGAGGTTAGA AGACAGCATC 
TCTGAAAATC AGGGTCCGGA ACAGTCAGAT GTAACCAATG GAGCCAGGAC TGAGGTTATT 
ACCAAAGATG GAAGTCCAAC ACCTTCTCCA CAACTTGTGG CTAGGCCAAC CTTCTCCCCG 
ATAAACCTCC AGTATTCTAC TTTGCCATCT AGCCCCACTG AACCATTGGC ATCTGAGCAA 
ATGGAAGGAA TGCTGCACCG CAAGCAGGAA ATGGATTCTC ACAACAAGAA GGCTGCGAAC 
AGGTCCTGGC AGAATGTTTA CTGTGCACTG AAAAGGGGCA GTCTGGGTTT CTACAAGGAT 
GCTAAAAGTG CAGCCAGTGG CGTTTCTTAT CATTCGGAGC CCTCCGTCCA ACTTAATGGA 
GCCCAGTGCA GTGTTGCTCA TGACTACAAG AAACGCAAGA ATGTCTTCAA GCTAAGCCTT 
CTTGATGGCA AAGAATATCT GTTCCAAGCT AAGGATGAGG CTGAAATGAG TAGTTGGATG 
CGAGTTATAA ACGCCTCCCT AACTTCCTCC CCGGGAGACC CCTCAGACCA TTCACCGGGC 
ATCATGAAGG GTATGACCCG GGCCATGACA ATGCCACCAG TATCACCCAA CAGTGGTACA 
GAGGGATCAG TCACATTGCG CAACAAGGAC GGGAAAGAAA AAGACCGGGA AAAGAGATTC 
AGTTTCTTTA AGAAGAAGTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

sptbn2 ( XM_004913695.4 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_004913752.1 Xenopus tropicalis spectrin, beta, non-erythrocytic 2 (sptbn2), transcript variant X2, mRNA.

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CloneID	OXa56730
Clone ID Related Accession (Same CDS sequence)	XM_004913695.4
Accession Version	XM_004913695.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7206bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1576512000000
Organism	Xenopus tropicalis(tropical clawed frog)
Product	spectrin beta chain, non-erythrocytic 2 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030680.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_004913695.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCAGTC CACTGTCACC GACAGATTAT GACAGCCTGG AAATCCAGCA GCAGTATAAT 
GACATCAACA ATCGATGGGA TCTGACAGAT GATGATTGGG ACAATGAGAA CAGTTCTGCA 
AGACTTTTTG AGAGGTCCCG GATCAAAGCA CTTGCAGATG AAAGAGAAGC TGTGCAGAAG 
AAGACATTTA CTAAATGGGT CAACTCTCAC TTGGCGAGGG TCACCTGTAG AATTTCTGAT 
CTCTACAATG ATTTACGAGA TGGACGGATG TTAATTCGAC TGTTAGAAGT GCTATCTGGA 
GAGCAGCTGC CAAAACCCAC AAAAGGTCGA ATGCGAATCC ACTGTTTGGA AAATGTAGAC 
AAAGCCCTTC AATTTTTAAA AGAGCAGAAG GTACATTTGG AGAACATGGG ATCACATGAC 
ATTGTGGATG GAAACCATCG ATTAACCCTA GGGTTAATCT GGACTATTAT TTTAAGGTTT 
CAGATCCAAG ATATCAGTGT AGAGACAGAA GATAATAAAG AAAAGAAGTC TGCTAAAGAT 
GCCTTACTGC TTTGGTGCCA GATGAAGACT GCTGGGTATC CAAATGTCAA TGTTCACAAC 
TTTACCACCA GCTGGCGAGA TGGACTTGCG TTTAATGCCA TTGTACACAA GCACAGACCA 
GATATAATTG ACTTTGACAC ACTGAAGAAG TCTAATGCAC ACCACAACCT CCAGAATGCC 
TTCAATGTAG CTGAAAGAGA GTTAGGTCTT ACTAAACTTC TGGATCCTGA AGATGTTAAT 
GTGGATCAGC CTGATGAGAA ATCCATCATA ACCTATGTGG CCACATTTTA TCACTATTTT 
TCCAAAATGA AAGCCCTAGC TGTGGAAGGA AAGCGTATTG GAAAGGTACT TGACCAAGCT 
ATTGAAGCTG ATCAGTTAAT TGAGAACTAT GAAGGACTGG CATCAGAACT CCTGCAATGG 
ATTGAACAGA TGATCATTAC TCTCAATGAC CGAAAACTGG CAAACTCTCT AAGTGGTGTT 
CAGAACCAGC TGCAGGCCTT CAACACCTAC AGGACTGTAG AAAAGCCGCC CAAGTTCACA 
GAAAAGGGCA ATTTGGAAGT TCTTCTCTTT ACCATACAGA GTAAAATGAG AGCTAATAAT 
CAAAAAGTGT ATACGCCCAG AGAAGGAAAG CTTATATCTG ATATAAACAA GGCATGGGAG 
CGACTAGAAA AGGCAGAGCA TGAACGTGAG CTTGCCCTTC GCAATGAACT TATACGCCAG 
GAAAAATTAG AGCAGTTGGC TGCACGATTT GACCGTAAAG CAGCCATGAG GGAAACCTGG 
CTAAGTGAAA ATCAGAGACT TGTAGCTCAG GATAACTTCG GTGTGGACTT AACTGCGGTG 
GAGGCAGCGC TACGGAAACA TGAAGCTATT GAAACTGATA TATTGGCTTA CAGTGAGCGC 
GTGCAAGCAG TGAATGCAGT GGCTGATGAG CTGGAAGCAG AGAATTACCA TGATGTCCGG 
AGGATTTTGG CTCGGAAGTG CAATGTGATT CGTCTCTCTG ATTATTTAAG AGAGCTTGTC 
ACTGCCAGGA GGCAAAGGCT TCATATGAAC CTAGAATTGC AGAGGATGTT CCAGGATTTA 
CATTATCTCA TGGACTGGAC TGAAGATATG AAGGGCCGTC TGCAGTCAGA AGATTATGGC 
AAGCATCTTC ATGGCGTGGA AGACCTTCTC CAGACTCACA CTCTTGTGGA GGCAGACATT 
GCAGTTCAGG CAGAACGTGT GAAATCAGTT ACAGCTGTAG CTCAGAAATT TATGACAGAA 
GATGAAGGCT ACAAGCCTTG CGACCCAGAG CTAGTGCGCA ACCGGGTGCA TCTCTTGGAA 
CAGCGCTATG CAGAGCTGTG TAATCTTTCT GCGGCTCGGA GATCAAAGCT GGAAGAATCC 
CGCCGTCTCT GGAAATTTTT CTGGGACATG GGAGAGGAGG AAGCCTGGAT CCGTGAGCAG 
AGTAGATTAC TACTTTCTGA TGACTATGGG AAAGACCTTA CCAGCTCACT TAGGTTGATC 
AGCAAGCATA ATGCCTTCAG AGGTGAGATG AGTGGACACC GTGGGCCGTT GCAGCAGACT 
GTGGCTGAGG GTGAGGAACT AATATCCAAA GGTCACTTTG GTGCAGGAGA GATTGAGGAA 
AGGATAAAAG AAGTGAAAGG CCAGTGGACA GAGATGGAGG CCTTAGCTGA AGACAGGGAA 
AGGAGGCTAA GAGAGGCCTA CAGCTATTAT CAGTTTCAAG CTGATGCCAA TGATGCTGAG 
GCTTGGATCC TGGACACTTT ACGCCTTGTT TCTACCAGTG AAGTAGGACA TGATGAGTAT 
TCCACACAGA CACTGGTGAA AAAGCACAAA GAAATAGAAG AGGAGATCCT TAGCCACCGA 
CCAGCTATTG ATGCGCTGCA TGAACAGGCC CGTGGCCTTC CACCTGCTTA CTCTCATTCA 
CCTGAAGTTG ATGGAAGGTT ACCAGCCCTG GAGCAGCGCT ATGAGGAATT GGTGGCTCTT 
GCTGCTCACC GCAAGCAGGC TCTGCAAGAT TCTCTGGCTC TTCATAAAAT GTTTAGTGAA 
GCTGATGCTT GCCTTTTGTG GATTGATGAG AAAGAACAGT GGCTTAATGT CACAGAGATT 
CCAGAAAAAC TTGAGGACCT AGAAGTAGTT CAACAAAGGT TTGAAACTCT AGAGGCAGAG 
ATGAATAATT TGGCATCCCG GATTGCATCT GTAAATGAAG TTGCTGGACA GTTGCTGACT 
ACAGACCAGA GAAATGAAAA CAGCATCCGA GACATGCAAG ATCAACTTAA TTCTCGGTGG 
AATGAATTCC GCAAGCTTGT TGACCAAAAG AAGGGTGCTT TGGATTCTGC TCTGGATATA 
CAGAATTATC ATTTGGAGTG CAATGAGACT GTGTGCTGGA TGAGGGAGAA GACAAAGGTT 
ATTGAATCCA CACAGAGCCT AGGGAATGAT CTTGCAGGAG TAACAGCCCT CCAGAGAAAA 
CTTACTGGAA TGGAGCGTGA TCTGAAAGCC ATTCAGGGAA AAGTGGGTGA CCTGCGTTCA 
GAGGCAGAAA AGCTTGCAGA TGGGCACCCA CAGAAAGCAC CGGCCATAAT TGGCCGCTTA 
TCAGATATTG ATGAGACCTG GGGAGAACTA AAGGACACTA TGAAAAGAAG AGAAGAAACT 
TTAGGTGAAG CAAGCAAGCT GCAGGGATTT TTGAGAGACC TTGATGATTT CCAGAGCTGG 
TTGTCCCGGG CCCAGACAGC AGTGGCATCT GAGGATGTTC CAGCTGACCT AGCAGAAGCA 
GAGCGCCTTC TTGCACAACA TGAAGGCATA AAAAATGAGA TTGATCATTA TGGAGCTGAC 
TACCACAGAG TGAGAGATGT TGGTGAAGAA GTCACTCGTG GGCAAACAGA TGCCCAATAT 
ATGTTCTTGC AACAGAGACT GAAGGCGCTA GACACTGGCT GGAATGAGCT TGGACAGATG 
TGGGAAAACA GGCACCAGTT GTTGTCCCAG GGTCACAGCT TCCAGCAGTT TTTGAAAGAT 
ACAAAACAAG TGGAGGGGTT CCTTAATAAC CAGGAGTATG TCCTTTCCCA TACCGAAATG 
CCAAGTACGC TACAGGCAGC TGAAGCTGCC ATCAAACGCC ATGAAGATTT CCTCACAACC 
ATGGATGCCA ATGAAGAGAA GATACAGGGT CTTGTAGAAT CTGGGAGGAA GCTAATTGAG 
GAACAGAATG TCAATTCTGA GAAGATTCAA GAAAAAGTCA CTTCTATTGA TGAGAGACAC 
AAAAAGAACC AAAAAACTGC TAGCGAAGTA TTAACTCGTT TGAAGGACAA CCGTGATCTT 
CAACATTTCC TTCAAGACTG TCAAGAGCTC ACCCTCTGGA TCAATGAAAA AATGCTTACT 
GCTCAAGATG TTTCTTATGA TGAAGCTCGA AACCTGCATA GCAAGTGGCA AAAGCATCAG 
GCATTCATGG CAGAACTGGC CTCAAATAAA GACTGGCTTG ACAAAATAGA CAAGGAAGGG 
CAGCAGCTCA TGTCAGAAAA ACCGGAGCTT GAACCAGTAG TGAGAGAAAA ACTAGACACT 
CTGCATAAGC TGTGGAATGA CTTGGAATCT ACCACACAAA GTAAAGCTCA GTGTCTGTTT 
GATGCCAACA GGGCAGAGTT ATTCACACAA AGCTGTTCTG CTCTGGAGAG TTGGCTTCAA 
AACCTCGAGT CACAACTACA GTCAGATCAT TATGGCAAAG ATCTGACCAG TGTCAACATC 
CTATTAAATA AACAGCAGAT GCTGGAGAAC CAAATGGAAG TGAGGGAGAA GGAGCTAGAA 
GCTATACAGT CCCAGGCACA GGCCCTTGGA CAGGAGGATG CCAACATAGT GGAGGTTGAC 
TCAAAGCATA AGGATGTAGC AGATAAATTT TCCAAGCTGC GTGCTCCTCT AAACAAGAGA 
AGAAAATTAC TTCTAGCCTC CAAGGAAGGA CATCAGTTTA ACAGGGACCT GGAAGATGAA 
ATACTTTGGG TAAAGGAGAA GATGCCAATG GCAATCTCCA CTGATCATGG AAAAGATCTC 
CCGACAGTCC AGCTTCTTAT CAAAAAGAAT CAGACACTCC AAAAGGAAAT TTTGGGACAT 
CAACCACGGA TTGATGACAT CCTGGAGAGA GGCAAATCTA TGATCCAAGA CAGCTCTCTG 
GACCCTCAAG AGATAGAAGA AAGGCTTGGG CTACTGAAAA GCACATGGGA AGAGCTGGCA 
GCAGAAGGAG GAAAGCGCCA TGCCCGTTTA GAGGAATCTC TTGCTGCTCA GCAGTTTTAT 
GCTGATGCGG CAGAAGCTGA GGCCTGGATG GGAGAGCAAG AGTTACATAT GATGTCTGAA 
GAAAAAGCCA AGGATGAGAT TGGTGCCCAG GCAATGCTGA AGAAACACTT AGTAATGGAA 
CAGGCTCTTG ATGACTATGC AGACAACATC CATCAGCTTG CCAATTCAAG CCGCGACATG 
GTCTCCAGAG GTCATCCAGA AAGTGATCGT ATTACCCTTC GTCAAGGGCA AGTGGACAAG 
CTATATGCCA GTCTTAAAGA CCTGGCAGAG GAGAGACAAG GAAAGCTACA AGAGCACCAC 
AGACTTTGCC AACTCAAGAG AGATGTCGAA GACCTTGAGC ACTGGATCTC TGAACGTGAG 
GTGGTTGCAG CTTCTCATGA ACTTGGGCAA GATTATGAGC ATGTAACGAT GCTGAGAGAC 
AAATTCCGTG AGTTCTCCCG TGACACCAGT AACATTGGTC AAGAGCGGGT GGATGCTGTC 
AATCTGATGG CAGATGATTT AATTTCATCT GGACATTCGG AAAATGCTAC TGTAGCAGAT 
TGGAAAGACC AGCTAAATGA GGCATGGGCT GATCTTCTTG AGATGATTGA CACTCGAAGT 
CAGATGCTGG CAGCCTCATA TGAACTTCAC CGTTTCTACC AGGATGCAAA AGAAACCCTA 
GGTCAGATCC AAGACAAGCA GAAACAAATA CCAGAGGAGA CAGGCAGAGA TCTGAATACA 
GCAGAAGCCA TGCAGCGCAT GCACACCGCT TTTGAACATG ATATCCAAGC CCTGAGTGCA 
CAGGTGAAGC AAGTCCAGGA AGATGCTTCT CGCCTCCAAA AAGCTTATGC TGGTGAAAAG 
GCAGCAGACA TTCATCGCCA TGAGCAGGCA GTATCGGAAG CTTGGGGAGA GTTGCAGAAT 
GCTAGTGGTG CCAGGCGGCA GCTTCTTCTT GACACTGTGG AGAAATTCCG TTTCTTCAGA 
ATGGTGCGAG ATCTCATGTT ATGGATGGAT GATATAAACC TGCTAATTGA TGCACAGGAG 
AAGCCAAGAG ATGTATCCTC AGCTGATTTA GTCATCCAAA ACCACCTAGG CATCAAGGCA 
GAAGTGGATG CGCGAGCTGA CAGCTTTACT ACATGCATAG ATCTTGGCAA CTCTCTCCTA 
GAGAAGAATC ATTATGCCTC TGACAAGATT TCAGAGAAAC TGGAGCAATT GCAGGAAAGA 
AGGCAGGAGA CTGAAAACAA ATGGAAAGAT AAAATGGCAT GGCTGAGAGT TGTCCACGAA 
GTGCTGATGT TTGGAAGAGA TGCAAGTGTA GCTGAGGCTT GGCTGGCCAG CCAGGAGCCC 
ATTGTCCGGT CGTCAGAACT TGGTGGCAAT GTCGATGAAG TAGAATCCCT GATAAAAAGA 
CACGAAGCCT TTCAGAAAGC AGTTTCTACA TGGGATGAGA GATTTGTTCT TCTTGAAAAA 
CTAACCACGT TAGAAGAAAT TGAAATGCGT CGGAAGCGGG AAGAGGAAGA AAGAAAGAAA 
AGACCACCAA CACCACCTCT ACCAGTAGAA GAGCCTCGAG AGGAAGTTGT AAAAACCCAA 
GTTTCCCAGA CTATTGAAGG GCAGACTGAT CCAGCCAATA TACTTCAAGA AAGGATAAAT 
GGCATGCAGA ATTCATTCCA GGAGCAAGTA TCTGTTAATG GACTGTGCAC AGAGTCTGAT 
TCTCCACAGC CATCGTCCGA TACACAGAGG TTAGAAGACA GCATCTCTGA AAATCAGGGT 
CCGGAACAGT CAGATGTAAC CAATGGAGCC AGGACTGAGG TTATTACCAA AGATGGAAGT 
CCAACACCTT CTCCACAACT TGTGGCTAGG CCAACCTTCT CCCCGATAAA CCTCCAGTAT 
TCTACTTTGC CATCTAGCCC CACTGAACCA TTGGCATCTG AGCAAATGGA AGGAATGCTG 
CACCGCAAGC AGGAAATGGA TTCTCACAAC AAGAAGGCTG CGAACAGGTC CTGGCAGAAT 
GTTTACTGTG CACTGAAAAG GGGCAGTCTG GGTTTCTACA AGGATGCTAA AAGTGCAGCC 
AGTGGCGTTT CTTATCATTC GGAGCCCTCC GTCCAACTTA ATGGAGCCCA GTGCAGTGTT 
GCTCATGACT ACAAGAAACG CAAGAATGTC TTCAAGCTAA GCCTTCTTGA TGGCAAAGAA 
TATCTGTTCC AAGCTAAGGA TGAGGCTGAA ATGAGTAGTT GGATGCGAGT TATAAACGCC 
TCCCTAACTT CCTCCCCGGG AGACCCCTCA GACCATTCAC CGGGCATCAT GAAGGGTATG 
ACCCGGGCCA TGACAATGCC ACCAGTATCA CCCAACAGTG GTACAGAGGG ATCAGTCACA 
TTGCGCAACA AGGACGGGAA AGAAAAAGAC CGGGAAAAGA GATTCAGTTT CTTTAAGAAG 
AAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004913752.1
CDS	238..7443
Translation	MSSPLSPTDYDSLEIQQQYNDINNRWDLTDDDWDNENSSARLFERSRIKALADEREAVQKKTFTKWVNSHLARVTCRISDLYNDLRDGRMLIRLLEVLSGEQLPKPTKGRMRIHCLENVDKALQFLKEQKVHLENMGSHDIVDGNHRLTLGLIWTIILRFQIQDISVETEDNKEKKSAKDALLLWCQMKTAGYPNVNVHNFTTSWRDGLAFNAIVHKHRPDIIDFDTLKKSNAHHNLQNAFNVAERELGLTKLLDPEDVNVDQPDEKSIITYVATFYHYFSKMKALAVEGKRIGKVLDQAIEADQLIENYEGLASELLQWIEQMIITLNDRKLANSLSGVQNQLQAFNTYRTVEKPPKFTEKGNLEVLLFTIQSKMRANNQKVYTPREGKLISDINKAWERLEKAEHERELALRNELIRQEKLEQLAARFDRKAAMRETWLSENQRLVAQDNFGVDLTAVEAALRKHEAIETDILAYSERVQAVNAVADELEAENYHDVRRILARKCNVIRLSDYLRELVTARRQRLHMNLELQRMFQDLHYLMDWTEDMKGRLQSEDYGKHLHGVEDLLQTHTLVEADIAVQAERVKSVTAVAQKFMTEDEGYKPCDPELVRNRVHLLEQRYAELCNLSAARRSKLEESRRLWKFFWDMGEEEAWIREQSRLLLSDDYGKDLTSSLRLISKHNAFRGEMSGHRGPLQQTVAEGEELISKGHFGAGEIEERIKEVKGQWTEMEALAEDRERRLREAYSYYQFQADANDAEAWILDTLRLVSTSEVGHDEYSTQTLVKKHKEIEEEILSHRPAIDALHEQARGLPPAYSHSPEVDGRLPALEQRYEELVALAAHRKQALQDSLALHKMFSEADACLLWIDEKEQWLNVTEIPEKLEDLEVVQQRFETLEAEMNNLASRIASVNEVAGQLLTTDQRNENSIRDMQDQLNSRWNEFRKLVDQKKGALDSALDIQNYHLECNETVCWMREKTKVIESTQSLGNDLAGVTALQRKLTGMERDLKAIQGKVGDLRSEAEKLADGHPQKAPAIIGRLSDIDETWGELKDTMKRREETLGEASKLQGFLRDLDDFQSWLSRAQTAVASEDVPADLAEAERLLAQHEGIKNEIDHYGADYHRVRDVGEEVTRGQTDAQYMFLQQRLKALDTGWNELGQMWENRHQLLSQGHSFQQFLKDTKQVEGFLNNQEYVLSHTEMPSTLQAAEAAIKRHEDFLTTMDANEEKIQGLVESGRKLIEEQNVNSEKIQEKVTSIDERHKKNQKTASEVLTRLKDNRDLQHFLQDCQELTLWINEKMLTAQDVSYDEARNLHSKWQKHQAFMAELASNKDWLDKIDKEGQQLMSEKPELEPVVREKLDTLHKLWNDLESTTQSKAQCLFDANRAELFTQSCSALESWLQNLESQLQSDHYGKDLTSVNILLNKQQMLENQMEVREKELEAIQSQAQALGQEDANIVEVDSKHKDVADKFSKLRAPLNKRRKLLLASKEGHQFNRDLEDEILWVKEKMPMAISTDHGKDLPTVQLLIKKNQTLQKEILGHQPRIDDILERGKSMIQDSSLDPQEIEERLGLLKSTWEELAAEGGKRHARLEESLAAQQFYADAAEAEAWMGEQELHMMSEEKAKDEIGAQAMLKKHLVMEQALDDYADNIHQLANSSRDMVSRGHPESDRITLRQGQVDKLYASLKDLAEERQGKLQEHHRLCQLKRDVEDLEHWISEREVVAASHELGQDYEHVTMLRDKFREFSRDTSNIGQERVDAVNLMADDLISSGHSENATVADWKDQLNEAWADLLEMIDTRSQMLAASYELHRFYQDAKETLGQIQDKQKQIPEETGRDLNTAEAMQRMHTAFEHDIQALSAQVKQVQEDASRLQKAYAGEKAADIHRHEQAVSEAWGELQNASGARRQLLLDTVEKFRFFRMVRDLMLWMDDINLLIDAQEKPRDVSSADLVIQNHLGIKAEVDARADSFTTCIDLGNSLLEKNHYASDKISEKLEQLQERRQETENKWKDKMAWLRVVHEVLMFGRDASVAEAWLASQEPIVRSSELGGNVDEVESLIKRHEAFQKAVSTWDERFVLLEKLTTLEEIEMRRKREEEERKKRPPTPPLPVEEPREEVVKTQVSQTIEGQTDPANILQERINGMQNSFQEQVSVNGLCTESDSPQPSSDTQRLEDSISENQGPEQSDVTNGARTEVITKDGSPTPSPQLVARPTFSPINLQYSTLPSSPTEPLASEQMEGMLHRKQEMDSHNKKAANRSWQNVYCALKRGSLGFYKDAKSAASGVSYHSEPSVQLNGAQCSVAHDYKKRKNVFKLSLLDGKEYLFQAKDEAEMSSWMRVINASLTSSPGDPSDHSPGIMKGMTRAMTMPPVSPNSGTEGSVTLRNKDGKEKDREKRFSFFKKK

Target ORF information:

RefSeq Version	XM_004913695.4
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis spectrin, beta, non-erythrocytic 2 (sptbn2), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004913695.4

ORF Insert Sequence:

ATGAGCAGTC CACTGTCACC GACAGATTAT GACAGCCTGG AAATCCAGCA GCAGTATAAT 
GACATCAACA ATCGATGGGA TCTGACAGAT GATGATTGGG ACAATGAGAA CAGTTCTGCA 
AGACTTTTTG AGAGGTCCCG GATCAAAGCA CTTGCAGATG AAAGAGAAGC TGTGCAGAAG 
AAGACATTTA CTAAATGGGT CAACTCTCAC TTGGCGAGGG TCACCTGTAG AATTTCTGAT 
CTCTACAATG ATTTACGAGA TGGACGGATG TTAATTCGAC TGTTAGAAGT GCTATCTGGA 
GAGCAGCTGC CAAAACCCAC AAAAGGTCGA ATGCGAATCC ACTGTTTGGA AAATGTAGAC 
AAAGCCCTTC AATTTTTAAA AGAGCAGAAG GTACATTTGG AGAACATGGG ATCACATGAC 
ATTGTGGATG GAAACCATCG ATTAACCCTA GGGTTAATCT GGACTATTAT TTTAAGGTTT 
CAGATCCAAG ATATCAGTGT AGAGACAGAA GATAATAAAG AAAAGAAGTC TGCTAAAGAT 
GCCTTACTGC TTTGGTGCCA GATGAAGACT GCTGGGTATC CAAATGTCAA TGTTCACAAC 
TTTACCACCA GCTGGCGAGA TGGACTTGCG TTTAATGCCA TTGTACACAA GCACAGACCA 
GATATAATTG ACTTTGACAC ACTGAAGAAG TCTAATGCAC ACCACAACCT CCAGAATGCC 
TTCAATGTAG CTGAAAGAGA GTTAGGTCTT ACTAAACTTC TGGATCCTGA AGATGTTAAT 
GTGGATCAGC CTGATGAGAA ATCCATCATA ACCTATGTGG CCACATTTTA TCACTATTTT 
TCCAAAATGA AAGCCCTAGC TGTGGAAGGA AAGCGTATTG GAAAGGTACT TGACCAAGCT 
ATTGAAGCTG ATCAGTTAAT TGAGAACTAT GAAGGACTGG CATCAGAACT CCTGCAATGG 
ATTGAACAGA TGATCATTAC TCTCAATGAC CGAAAACTGG CAAACTCTCT AAGTGGTGTT 
CAGAACCAGC TGCAGGCCTT CAACACCTAC AGGACTGTAG AAAAGCCGCC CAAGTTCACA 
GAAAAGGGCA ATTTGGAAGT TCTTCTCTTT ACCATACAGA GTAAAATGAG AGCTAATAAT 
CAAAAAGTGT ATACGCCCAG AGAAGGAAAG CTTATATCTG ATATAAACAA GGCATGGGAG 
CGACTAGAAA AGGCAGAGCA TGAACGTGAG CTTGCCCTTC GCAATGAACT TATACGCCAG 
GAAAAATTAG AGCAGTTGGC TGCACGATTT GACCGTAAAG CAGCCATGAG GGAAACCTGG 
CTAAGTGAAA ATCAGAGACT TGTAGCTCAG GATAACTTCG GTGTGGACTT AACTGCGGTG 
GAGGCAGCGC TACGGAAACA TGAAGCTATT GAAACTGATA TATTGGCTTA CAGTGAGCGC 
GTGCAAGCAG TGAATGCAGT GGCTGATGAG CTGGAAGCAG AGAATTACCA TGATGTCCGG 
AGGATTTTGG CTCGGAAGTG CAATGTGATT CGTCTCTCTG ATTATTTAAG AGAGCTTGTC 
ACTGCCAGGA GGCAAAGGCT TCATATGAAC CTAGAATTGC AGAGGATGTT CCAGGATTTA 
CATTATCTCA TGGACTGGAC TGAAGATATG AAGGGCCGTC TGCAGTCAGA AGATTATGGC 
AAGCATCTTC ATGGCGTGGA AGACCTTCTC CAGACTCACA CTCTTGTGGA GGCAGACATT 
GCAGTTCAGG CAGAACGTGT GAAATCAGTT ACAGCTGTAG CTCAGAAATT TATGACAGAA 
GATGAAGGCT ACAAGCCTTG CGACCCAGAG CTAGTGCGCA ACCGGGTGCA TCTCTTGGAA 
CAGCGCTATG CAGAGCTGTG TAATCTTTCT GCGGCTCGGA GATCAAAGCT GGAAGAATCC 
CGCCGTCTCT GGAAATTTTT CTGGGACATG GGAGAGGAGG AAGCCTGGAT CCGTGAGCAG 
AGTAGATTAC TACTTTCTGA TGACTATGGG AAAGACCTTA CCAGCTCACT TAGGTTGATC 
AGCAAGCATA ATGCCTTCAG AGGTGAGATG AGTGGACACC GTGGGCCGTT GCAGCAGACT 
GTGGCTGAGG GTGAGGAACT AATATCCAAA GGTCACTTTG GTGCAGGAGA GATTGAGGAA 
AGGATAAAAG AAGTGAAAGG CCAGTGGACA GAGATGGAGG CCTTAGCTGA AGACAGGGAA 
AGGAGGCTAA GAGAGGCCTA CAGCTATTAT CAGTTTCAAG CTGATGCCAA TGATGCTGAG 
GCTTGGATCC TGGACACTTT ACGCCTTGTT TCTACCAGTG AAGTAGGACA TGATGAGTAT 
TCCACACAGA CACTGGTGAA AAAGCACAAA GAAATAGAAG AGGAGATCCT TAGCCACCGA 
CCAGCTATTG ATGCGCTGCA TGAACAGGCC CGTGGCCTTC CACCTGCTTA CTCTCATTCA 
CCTGAAGTTG ATGGAAGGTT ACCAGCCCTG GAGCAGCGCT ATGAGGAATT GGTGGCTCTT 
GCTGCTCACC GCAAGCAGGC TCTGCAAGAT TCTCTGGCTC TTCATAAAAT GTTTAGTGAA 
GCTGATGCTT GCCTTTTGTG GATTGATGAG AAAGAACAGT GGCTTAATGT CACAGAGATT 
CCAGAAAAAC TTGAGGACCT AGAAGTAGTT CAACAAAGGT TTGAAACTCT AGAGGCAGAG 
ATGAATAATT TGGCATCCCG GATTGCATCT GTAAATGAAG TTGCTGGACA GTTGCTGACT 
ACAGACCAGA GAAATGAAAA CAGCATCCGA GACATGCAAG ATCAACTTAA TTCTCGGTGG 
AATGAATTCC GCAAGCTTGT TGACCAAAAG AAGGGTGCTT TGGATTCTGC TCTGGATATA 
CAGAATTATC ATTTGGAGTG CAATGAGACT GTGTGCTGGA TGAGGGAGAA GACAAAGGTT 
ATTGAATCCA CACAGAGCCT AGGGAATGAT CTTGCAGGAG TAACAGCCCT CCAGAGAAAA 
CTTACTGGAA TGGAGCGTGA TCTGAAAGCC ATTCAGGGAA AAGTGGGTGA CCTGCGTTCA 
GAGGCAGAAA AGCTTGCAGA TGGGCACCCA CAGAAAGCAC CGGCCATAAT TGGCCGCTTA 
TCAGATATTG ATGAGACCTG GGGAGAACTA AAGGACACTA TGAAAAGAAG AGAAGAAACT 
TTAGGTGAAG CAAGCAAGCT GCAGGGATTT TTGAGAGACC TTGATGATTT CCAGAGCTGG 
TTGTCCCGGG CCCAGACAGC AGTGGCATCT GAGGATGTTC CAGCTGACCT AGCAGAAGCA 
GAGCGCCTTC TTGCACAACA TGAAGGCATA AAAAATGAGA TTGATCATTA TGGAGCTGAC 
TACCACAGAG TGAGAGATGT TGGTGAAGAA GTCACTCGTG GGCAAACAGA TGCCCAATAT 
ATGTTCTTGC AACAGAGACT GAAGGCGCTA GACACTGGCT GGAATGAGCT TGGACAGATG 
TGGGAAAACA GGCACCAGTT GTTGTCCCAG GGTCACAGCT TCCAGCAGTT TTTGAAAGAT 
ACAAAACAAG TGGAGGGGTT CCTTAATAAC CAGGAGTATG TCCTTTCCCA TACCGAAATG 
CCAAGTACGC TACAGGCAGC TGAAGCTGCC ATCAAACGCC ATGAAGATTT CCTCACAACC 
ATGGATGCCA ATGAAGAGAA GATACAGGGT CTTGTAGAAT CTGGGAGGAA GCTAATTGAG 
GAACAGAATG TCAATTCTGA GAAGATTCAA GAAAAAGTCA CTTCTATTGA TGAGAGACAC 
AAAAAGAACC AAAAAACTGC TAGCGAAGTA TTAACTCGTT TGAAGGACAA CCGTGATCTT 
CAACATTTCC TTCAAGACTG TCAAGAGCTC ACCCTCTGGA TCAATGAAAA AATGCTTACT 
GCTCAAGATG TTTCTTATGA TGAAGCTCGA AACCTGCATA GCAAGTGGCA AAAGCATCAG 
GCATTCATGG CAGAACTGGC CTCAAATAAA GACTGGCTTG ACAAAATAGA CAAGGAAGGG 
CAGCAGCTCA TGTCAGAAAA ACCGGAGCTT GAACCAGTAG TGAGAGAAAA ACTAGACACT 
CTGCATAAGC TGTGGAATGA CTTGGAATCT ACCACACAAA GTAAAGCTCA GTGTCTGTTT 
GATGCCAACA GGGCAGAGTT ATTCACACAA AGCTGTTCTG CTCTGGAGAG TTGGCTTCAA 
AACCTCGAGT CACAACTACA GTCAGATCAT TATGGCAAAG ATCTGACCAG TGTCAACATC 
CTATTAAATA AACAGCAGAT GCTGGAGAAC CAAATGGAAG TGAGGGAGAA GGAGCTAGAA 
GCTATACAGT CCCAGGCACA GGCCCTTGGA CAGGAGGATG CCAACATAGT GGAGGTTGAC 
TCAAAGCATA AGGATGTAGC AGATAAATTT TCCAAGCTGC GTGCTCCTCT AAACAAGAGA 
AGAAAATTAC TTCTAGCCTC CAAGGAAGGA CATCAGTTTA ACAGGGACCT GGAAGATGAA 
ATACTTTGGG TAAAGGAGAA GATGCCAATG GCAATCTCCA CTGATCATGG AAAAGATCTC 
CCGACAGTCC AGCTTCTTAT CAAAAAGAAT CAGACACTCC AAAAGGAAAT TTTGGGACAT 
CAACCACGGA TTGATGACAT CCTGGAGAGA GGCAAATCTA TGATCCAAGA CAGCTCTCTG 
GACCCTCAAG AGATAGAAGA AAGGCTTGGG CTACTGAAAA GCACATGGGA AGAGCTGGCA 
GCAGAAGGAG GAAAGCGCCA TGCCCGTTTA GAGGAATCTC TTGCTGCTCA GCAGTTTTAT 
GCTGATGCGG CAGAAGCTGA GGCCTGGATG GGAGAGCAAG AGTTACATAT GATGTCTGAA 
GAAAAAGCCA AGGATGAGAT TGGTGCCCAG GCAATGCTGA AGAAACACTT AGTAATGGAA 
CAGGCTCTTG ATGACTATGC AGACAACATC CATCAGCTTG CCAATTCAAG CCGCGACATG 
GTCTCCAGAG GTCATCCAGA AAGTGATCGT ATTACCCTTC GTCAAGGGCA AGTGGACAAG 
CTATATGCCA GTCTTAAAGA CCTGGCAGAG GAGAGACAAG GAAAGCTACA AGAGCACCAC 
AGACTTTGCC AACTCAAGAG AGATGTCGAA GACCTTGAGC ACTGGATCTC TGAACGTGAG 
GTGGTTGCAG CTTCTCATGA ACTTGGGCAA GATTATGAGC ATGTAACGAT GCTGAGAGAC 
AAATTCCGTG AGTTCTCCCG TGACACCAGT AACATTGGTC AAGAGCGGGT GGATGCTGTC 
AATCTGATGG CAGATGATTT AATTTCATCT GGACATTCGG AAAATGCTAC TGTAGCAGAT 
TGGAAAGACC AGCTAAATGA GGCATGGGCT GATCTTCTTG AGATGATTGA CACTCGAAGT 
CAGATGCTGG CAGCCTCATA TGAACTTCAC CGTTTCTACC AGGATGCAAA AGAAACCCTA 
GGTCAGATCC AAGACAAGCA GAAACAAATA CCAGAGGAGA CAGGCAGAGA TCTGAATACA 
GCAGAAGCCA TGCAGCGCAT GCACACCGCT TTTGAACATG ATATCCAAGC CCTGAGTGCA 
CAGGTGAAGC AAGTCCAGGA AGATGCTTCT CGCCTCCAAA AAGCTTATGC TGGTGAAAAG 
GCAGCAGACA TTCATCGCCA TGAGCAGGCA GTATCGGAAG CTTGGGGAGA GTTGCAGAAT 
GCTAGTGGTG CCAGGCGGCA GCTTCTTCTT GACACTGTGG AGAAATTCCG TTTCTTCAGA 
ATGGTGCGAG ATCTCATGTT ATGGATGGAT GATATAAACC TGCTAATTGA TGCACAGGAG 
AAGCCAAGAG ATGTATCCTC AGCTGATTTA GTCATCCAAA ACCACCTAGG CATCAAGGCA 
GAAGTGGATG CGCGAGCTGA CAGCTTTACT ACATGCATAG ATCTTGGCAA CTCTCTCCTA 
GAGAAGAATC ATTATGCCTC TGACAAGATT TCAGAGAAAC TGGAGCAATT GCAGGAAAGA 
AGGCAGGAGA CTGAAAACAA ATGGAAAGAT AAAATGGCAT GGCTGAGAGT TGTCCACGAA 
GTGCTGATGT TTGGAAGAGA TGCAAGTGTA GCTGAGGCTT GGCTGGCCAG CCAGGAGCCC 
ATTGTCCGGT CGTCAGAACT TGGTGGCAAT GTCGATGAAG TAGAATCCCT GATAAAAAGA 
CACGAAGCCT TTCAGAAAGC AGTTTCTACA TGGGATGAGA GATTTGTTCT TCTTGAAAAA 
CTAACCACGT TAGAAGAAAT TGAAATGCGT CGGAAGCGGG AAGAGGAAGA AAGAAAGAAA 
AGACCACCAA CACCACCTCT ACCAGTAGAA GAGCCTCGAG AGGAAGTTGT AAAAACCCAA 
GTTTCCCAGA CTATTGAAGG GCAGACTGAT CCAGCCAATA TACTTCAAGA AAGGATAAAT 
GGCATGCAGA ATTCATTCCA GGAGCAAGTA TCTGTTAATG GACTGTGCAC AGAGTCTGAT 
TCTCCACAGC CATCGTCCGA TACACAGAGG TTAGAAGACA GCATCTCTGA AAATCAGGGT 
CCGGAACAGT CAGATGTAAC CAATGGAGCC AGGACTGAGG TTATTACCAA AGATGGAAGT 
CCAACACCTT CTCCACAACT TGTGGCTAGG CCAACCTTCT CCCCGATAAA CCTCCAGTAT 
TCTACTTTGC CATCTAGCCC CACTGAACCA TTGGCATCTG AGCAAATGGA AGGAATGCTG 
CACCGCAAGC AGGAAATGGA TTCTCACAAC AAGAAGGCTG CGAACAGGTC CTGGCAGAAT 
GTTTACTGTG CACTGAAAAG GGGCAGTCTG GGTTTCTACA AGGATGCTAA AAGTGCAGCC 
AGTGGCGTTT CTTATCATTC GGAGCCCTCC GTCCAACTTA ATGGAGCCCA GTGCAGTGTT 
GCTCATGACT ACAAGAAACG CAAGAATGTC TTCAAGCTAA GCCTTCTTGA TGGCAAAGAA 
TATCTGTTCC AAGCTAAGGA TGAGGCTGAA ATGAGTAGTT GGATGCGAGT TATAAACGCC 
TCCCTAACTT CCTCCCCGGG AGACCCCTCA GACCATTCAC CGGGCATCAT GAAGGGTATG 
ACCCGGGCCA TGACAATGCC ACCAGTATCA CCCAACAGTG GTACAGAGGG ATCAGTCACA 
TTGCGCAACA AGGACGGGAA AGAAAAAGAC CGGGAAAAGA GATTCAGTTT CTTTAAGAAG 
AAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

sptbn2 ( XM_012962405.3 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012817859.1 Xenopus tropicalis spectrin, beta, non-erythrocytic 2 (sptbn2), transcript variant X3, mRNA.

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CloneID	OXa56731
Clone ID Related Accession (Same CDS sequence)	XM_012962405.3
Accession Version	XM_012962405.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7314bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1576512000000
Organism	Xenopus tropicalis(tropical clawed frog)
Product	spectrin beta chain, non-erythrocytic 2 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030680.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012962405.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCAGTC CACTGTCACC GACAGATTAT GACAGCCTGG AAATCCAGCA GCAGTATAAT 
GACATCAACA ATCGATGGGA TCTGACAGAT GATGATTGGG ACAATGAGAA CAGTTCTGCA 
AGACTTTTTG AGAGGTCCCG GATCAAAGCA CTTGCAGAAA TGGATTTTGT CCCCCGATCT 
GGCTCCCCTC TCCTATCCCC TGCGGCTTTT GTAAATGCAG TGCAATACGC TAACGTGCTG 
GAAGGAAGGT TCAAGCAGCT GCAAGATGAA AGAGAAGCTG TGCAGAAGAA GACATTTACT 
AAATGGGTCA ACTCTCACTT GGCGAGGGTC ACCTGTAGAA TTTCTGATCT CTACAATGAT 
TTACGAGATG GACGGATGTT AATTCGACTG TTAGAAGTGC TATCTGGAGA GCAGCTGCCA 
AAACCCACAA AAGGTCGAAT GCGAATCCAC TGTTTGGAAA ATGTAGACAA AGCCCTTCAA 
TTTTTAAAAG AGCAGAAGGT ACATTTGGAG AACATGGGAT CACATGACAT TGTGGATGGA 
AACCATCGAT TAACCCTAGG GTTAATCTGG ACTATTATTT TAAGGTTTCA GATCCAAGAT 
ATCAGTGTAG AGACAGAAGA TAATAAAGAA AAGAAGTCTG CTAAAGATGC CTTACTGCTT 
TGGTGCCAGA TGAAGACTGC TGGGTATCCA AATGTCAATG TTCACAACTT TACCACCAGC 
TGGCGAGATG GACTTGCGTT TAATGCCATT GTACACAAGC ACAGACCAGA TATAATTGAC 
TTTGACACAC TGAAGAAGTC TAATGCACAC CACAACCTCC AGAATGCCTT CAATGTAGCT 
GAAAGAGAGT TAGGTCTTAC TAAACTTCTG GATCCTGAAG ATGTTAATGT GGATCAGCCT 
GATGAGAAAT CCATCATAAC CTATGTGGCC ACATTTTATC ACTATTTTTC CAAAATGAAA 
GCCCTAGCTG TGGAAGGAAA GCGTATTGGA AAGGTACTTG ACCAAGCTAT TGAAGCTGAT 
CAGTTAATTG AGAACTATGA AGGACTGGCA TCAGAACTCC TGCAATGGAT TGAACAGATG 
ATCATTACTC TCAATGACCG AAAACTGGCA AACTCTCTAA GTGGTGTTCA GAACCAGCTG 
CAGGCCTTCA ACACCTACAG GACTGTAGAA AAGCCGCCCA AGTTCACAGA AAAGGGCAAT 
TTGGAAGTTC TTCTCTTTAC CATACAGAGT AAAATGAGAG CTAATAATCA AAAAGTGTAT 
ACGCCCAGAG AAGGAAAGCT TATATCTGAT ATAAACAAGG CATGGGAGCG ACTAGAAAAG 
GCAGAGCATG AACGTGAGCT TGCCCTTCGC AATGAACTTA TACGCCAGGA AAAATTAGAG 
CAGTTGGCTG CACGATTTGA CCGTAAAGCA GCCATGAGGG AAACCTGGCT AAGTGAAAAT 
CAGAGACTTG TAGCTCAGGA TAACTTCGGT GTGGACTTAA CTGCGGTGGA GGCAGCGCTA 
CGGAAACATG AAGCTATTGA AACTGATATA TTGGCTTACA GTGAGCGCGT GCAAGCAGTG 
AATGCAGTGG CTGATGAGCT GGAAGCAGAG AATTACCATG ATGTCCGGAG GATTTTGGCT 
CGGAAGTGCA ATGTGATTCG TCTCTCTGAT TATTTAAGAG AGCTTGTCAC TGCCAGGAGG 
CAAAGGCTTC ATATGAACCT AGAATTGCAG AGGATGTTCC AGGATTTACA TTATCTCATG 
GACTGGACTG AAGATATGAA GGGCCGTCTG CAGTCAGAAG ATTATGGCAA GCATCTTCAT 
GGCGTGGAAG ACCTTCTCCA GACTCACACT CTTGTGGAGG CAGACATTGC AGTTCAGGCA 
GAACGTGTGA AATCAGTTAC AGCTGTAGCT CAGAAATTTA TGACAGAAGA TGAAGGCTAC 
AAGCCTTGCG ACCCAGAGCT AGTGCGCAAC CGGGTGCATC TCTTGGAACA GCGCTATGCA 
GAGCTGTGTA ATCTTTCTGC GGCTCGGAGA TCAAAGCTGG AAGAATCCCG CCGTCTCTGG 
AAATTTTTCT GGGACATGGG AGAGGAGGAA GCCTGGATCC GTGAGCAGAG TAGATTACTA 
CTTTCTGATG ACTATGGGAA AGACCTTACC AGCTCACTTA GGTTGATCAG CAAGCATAAT 
GCCTTCAGAG GTGAGATGAG TGGACACCGT GGGCCGTTGC AGCAGACTGT GGCTGAGGGT 
GAGGAACTAA TATCCAAAGG TCACTTTGGT GCAGGAGAGA TTGAGGAAAG GATAAAAGAA 
GTGAAAGGCC AGTGGACAGA GATGGAGGCC TTAGCTGAAG ACAGGGAAAG GAGGCTAAGA 
GAGGCCTACA GCTATTATCA GTTTCAAGCT GATGCCAATG ATGCTGAGGC TTGGATCCTG 
GACACTTTAC GCCTTGTTTC TACCAGTGAA GTAGGACATG ATGAGTATTC CACACAGACA 
CTGGTGAAAA AGCACAAAGA AATAGAAGAG GAGATCCTTA GCCACCGACC AGCTATTGAT 
GCGCTGCATG AACAGGCCCG TGGCCTTCCA CCTGCTTACT CTCATTCACC TGAAGTTGAT 
GGAAGGTTAC CAGCCCTGGA GCAGCGCTAT GAGGAATTGG TGGCTCTTGC TGCTCACCGC 
AAGCAGGCTC TGCAAGATTC TCTGGCTCTT CATAAAATGT TTAGTGAAGC TGATGCTTGC 
CTTTTGTGGA TTGATGAGAA AGAACAGTGG CTTAATGTCA CAGAGATTCC AGAAAAACTT 
GAGGACCTAG AAGTAGTTCA ACAAAGGTTT GAAACTCTAG AGGCAGAGAT GAATAATTTG 
GCATCCCGGA TTGCATCTGT AAATGAAGTT GCTGGACAGT TGCTGACTAC AGACCAGAGA 
AATGAAAACA GCATCCGAGA CATGCAAGAT CAACTTAATT CTCGGTGGAA TGAATTCCGC 
AAGCTTGTTG ACCAAAAGAA GGGTGCTTTG GATTCTGCTC TGGATATACA GAATTATCAT 
TTGGAGTGCA ATGAGACTGT GTGCTGGATG AGGGAGAAGA CAAAGGTTAT TGAATCCACA 
CAGAGCCTAG GGAATGATCT TGCAGGAGTA ACAGCCCTCC AGAGAAAACT TACTGGAATG 
GAGCGTGATC TGAAAGCCAT TCAGGGAAAA GTGGGTGACC TGCGTTCAGA GGCAGAAAAG 
CTTGCAGATG GGCACCCACA GAAAGCACCG GCCATAATTG GCCGCTTATC AGATATTGAT 
GAGACCTGGG GAGAACTAAA GGACACTATG AAAAGAAGAG AAGAAACTTT AGGTGAAGCA 
AGCAAGCTGC AGGGATTTTT GAGAGACCTT GATGATTTCC AGAGCTGGTT GTCCCGGGCC 
CAGACAGCAG TGGCATCTGA GGATGTTCCA GCTGACCTAG CAGAAGCAGA GCGCCTTCTT 
GCACAACATG AAGGCATAAA AAATGAGATT GATCATTATG GAGCTGACTA CCACAGAGTG 
AGAGATGTTG GTGAAGAAGT CACTCGTGGG CAAACAGATG CCCAATATAT GTTCTTGCAA 
CAGAGACTGA AGGCGCTAGA CACTGGCTGG AATGAGCTTG GACAGATGTG GGAAAACAGG 
CACCAGTTGT TGTCCCAGGG TCACAGCTTC CAGCAGTTTT TGAAAGATAC AAAACAAGTG 
GAGGGGTTCC TTAATAACCA GGAGTATGTC CTTTCCCATA CCGAAATGCC AAGTACGCTA 
CAGGCAGCTG AAGCTGCCAT CAAACGCCAT GAAGATTTCC TCACAACCAT GGATGCCAAT 
GAAGAGAAGA TACAGGGTCT TGTAGAATCT GGGAGGAAGC TAATTGAGGA ACAGAATGTC 
AATTCTGAGA AGATTCAAGA AAAAGTCACT TCTATTGATG AGAGACACAA AAAGAACCAA 
AAAACTGCTA GCGAAGTATT AACTCGTTTG AAGGACAACC GTGATCTTCA ACATTTCCTT 
CAAGACTGTC AAGAGCTCAC CCTCTGGATC AATGAAAAAA TGCTTACTGC TCAAGATGTT 
TCTTATGATG AAGCTCGAAA CCTGCATAGC AAGTGGCAAA AGCATCAGGC ATTCATGGCA 
GAACTGGCCT CAAATAAAGA CTGGCTTGAC AAAATAGACA AGGAAGGGCA GCAGCTCATG 
TCAGAAAAAC CGGAGCTTGA ACCAGTAGTG AGAGAAAAAC TAGACACTCT GCATAAGCTG 
TGGAATGACT TGGAATCTAC CACACAAAGT AAAGCTCAGT GTCTGTTTGA TGCCAACAGG 
GCAGAGTTAT TCACACAAAG CTGTTCTGCT CTGGAGAGTT GGCTTCAAAA CCTCGAGTCA 
CAACTACAGT CAGATCATTA TGGCAAAGAT CTGACCAGTG TCAACATCCT ATTAAATAAA 
CAGCAGATGC TGGAGAACCA AATGGAAGTG AGGGAGAAGG AGCTAGAAGC TATACAGTCC 
CAGGCACAGG CCCTTGGACA GGAGGATGCC AACATAGTGG AGGTTGACTC AAAGCATAAG 
GATGTAGCAG ATAAATTTTC CAAGCTGCGT GCTCCTCTAA ACAAGAGAAG AAAATTACTT 
CTAGCCTCCA AGGAAGGACA TCAGTTTAAC AGGGACCTGG AAGATGAAAT ACTTTGGGTA 
AAGGAGAAGA TGCCAATGGC AATCTCCACT GATCATGGAA AAGATCTCCC GACAGTCCAG 
CTTCTTATCA AAAAGAATCA GACACTCCAA AAGGAAATTT TGGGACATCA ACCACGGATT 
GATGACATCC TGGAGAGAGG CAAATCTATG ATCCAAGACA GCTCTCTGGA CCCTCAAGAG 
ATAGAAGAAA GGCTTGGGCT ACTGAAAAGC ACATGGGAAG AGCTGGCAGC AGAAGGAGGA 
AAGCGCCATG CCCGTTTAGA GGAATCTCTT GCTGCTCAGC AGTTTTATGC TGATGCGGCA 
GAAGCTGAGG CCTGGATGGG AGAGCAAGAG TTACATATGA TGTCTGAAGA AAAAGCCAAG 
GATGAGATTG GTGCCCAGGC AATGCTGAAG AAACACTTAG TAATGGAACA GGCTCTTGAT 
GACTATGCAG ACAACATCCA TCAGCTTGCC AATTCAAGCC GCGACATGGT CTCCAGAGGT 
CATCCAGAAA GTGATCGTAT TACCCTTCGT CAAGGGCAAG TGGACAAGCT ATATGCCAGT 
CTTAAAGACC TGGCAGAGGA GAGACAAGGA AAGCTACAAG AGCACCACAG ACTTTGCCAA 
CTCAAGAGAG ATGTCGAAGA CCTTGAGCAC TGGATCTCTG AACGTGAGGT GGTTGCAGCT 
TCTCATGAAC TTGGGCAAGA TTATGAGCAT GTAACGATGC TGAGAGACAA ATTCCGTGAG 
TTCTCCCGTG ACACCAGTAA CATTGGTCAA GAGCGGGTGG ATGCTGTCAA TCTGATGGCA 
GATGATTTAA TTTCATCTGG ACATTCGGAA AATGCTACTG TAGCAGATTG GAAAGACCAG 
CTAAATGAGG CATGGGCTGA TCTTCTTGAG ATGATTGACA CTCGAAGTCA GATGCTGGCA 
GCCTCATATG AACTTCACCG TTTCTACCAG GATGCAAAAG AAACCCTAGG TCAGATCCAA 
GACAAGCAGA AACAAATACC AGAGGAGACA GGCAGAGATC TGAATACAGC AGAAGCCATG 
CAGCGCATGC ACACCGCTTT TGAACATGAT ATCCAAGCCC TGAGTGCACA GGTGAAGCAA 
GTCCAGGAAG ATGCTTCTCG CCTCCAAAAA GCTTATGCTG GTGAAAAGGC AGCAGACATT 
CATCGCCATG AGCAGGCAGT ATCGGAAGCT TGGGGAGAGT TGCAGAATGC TAGTGGTGCC 
AGGCGGCAGC TTCTTCTTGA CACTGTGGAG AAATTCCGTT TCTTCAGAAT GGTGCGAGAT 
CTCATGTTAT GGATGGATGA TATAAACCTG CTAATTGATG CACAGGAGAA GCCAAGAGAT 
GTATCCTCAG CTGATTTAGT CATCCAAAAC CACCTAGGCA TCAAGGCAGA AGTGGATGCG 
CGAGCTGACA GCTTTACTAC ATGCATAGAT CTTGGCAACT CTCTCCTAGA GAAGAATCAT 
TATGCCTCTG ACAAGATTTC AGAGAAACTG GAGCAATTGC AGGAAAGAAG GCAGGAGACT 
GAAAACAAAT GGAAAGATAA AATGGCATGG CTGAGAGTTG TCCACGAAGT GCTGATGTTT 
GGAAGAGATG CAAGTGTAGC TGAGGCTTGG CTGGCCAGCC AGGAGCCCAT TGTCCGGTCG 
TCAGAACTTG GTGGCAATGT CGATGAAGTA GAATCCCTGA TAAAAAGACA CGAAGCCTTT 
CAGAAAGCAG TTTCTACATG GGATGAGAGA TTTGTTCTTC TTGAAAAACT AACCACGTTA 
GAAGAAATTG AAATGCGTCG GAAGCGGGAA GAGGAAGAAA GAAAGAAAAG ACCACCAACA 
CCACCTCTAC CAGTAGAAGA GCCTCGAGAG GAAGTTGTAA AAACCCAAGT TTCCCAGACT 
ATTGAAGGGC AGACTGATCC AGCCAATATA CTTCAAGAAA GGATAAATGG CATGCAGAAT 
TCATTCCAGG AGCAAGTATC TGTTAATGGA CTGTGCACAG AGTCTGATTC TCCACAGCCA 
TCGTCCGATA CACAGAGGTT AGAAGACAGC ATCTCTGAAA ATCAGGGTCC GGAACAGTCA 
GATGTAACCA ATGGAGCCAG GACTGAGGTT ATTACCAAAG ATGGAAGTCC AACACCTTCT 
CCACAACTTG TGGCTAGGCC AACCTTCTCC CCGATAAACC TCCAGTATTC TACTTTGCCA 
TCTAGCCCCA CTGAACCATT GGCATCTGAG CAAATGGAAG GAATGCTGCA CCGCAAGCAG 
GAAATGGATT CTCACAACAA GAAGGCTGCG AACAGGTCCT GGCAGAATGT TTACTGTGCA 
CTGAAAAGGG GCAGTCTGGG TTTCTACAAG GATGCTAAAA GTGCAGCCAG TGGCGTTTCT 
TATCATTCGG AGCCCTCCGT CCAACTTAAT GGAGCCCAGT GCAGTGTTGC TCATGACTAC 
AAGAAACGCA AGAATGTCTT CAAGCTAAGC CTTCTTGATG GCAAAGAATA TCTGTTCCAA 
GCTAAGGATG AGGCTGAAAT GAGTAGTTGG ATGCGAGTTA TAAACGCCTC CCTAACTTCC 
TCCCCGGGAG ACCCCTCAGA CCATTCACCG GGCATCATGA AGGGTATGAC CCGGGCCATG 
ACAATGCCAC CAGTATCACC CAACAGTGGT ACAGAGGGAT CAGTCACATT GCGCAACAAG 
GACGGGAAAG AAAAAGACCG GGAAAAGAGA TTCAGTTTCT TTAAGAAGAA GTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012817859.1
CDS	238..7551
Translation	MSSPLSPTDYDSLEIQQQYNDINNRWDLTDDDWDNENSSARLFERSRIKALAEMDFVPRSGSPLLSPAAFVNAVQYANVLEGRFKQLQDEREAVQKKTFTKWVNSHLARVTCRISDLYNDLRDGRMLIRLLEVLSGEQLPKPTKGRMRIHCLENVDKALQFLKEQKVHLENMGSHDIVDGNHRLTLGLIWTIILRFQIQDISVETEDNKEKKSAKDALLLWCQMKTAGYPNVNVHNFTTSWRDGLAFNAIVHKHRPDIIDFDTLKKSNAHHNLQNAFNVAERELGLTKLLDPEDVNVDQPDEKSIITYVATFYHYFSKMKALAVEGKRIGKVLDQAIEADQLIENYEGLASELLQWIEQMIITLNDRKLANSLSGVQNQLQAFNTYRTVEKPPKFTEKGNLEVLLFTIQSKMRANNQKVYTPREGKLISDINKAWERLEKAEHERELALRNELIRQEKLEQLAARFDRKAAMRETWLSENQRLVAQDNFGVDLTAVEAALRKHEAIETDILAYSERVQAVNAVADELEAENYHDVRRILARKCNVIRLSDYLRELVTARRQRLHMNLELQRMFQDLHYLMDWTEDMKGRLQSEDYGKHLHGVEDLLQTHTLVEADIAVQAERVKSVTAVAQKFMTEDEGYKPCDPELVRNRVHLLEQRYAELCNLSAARRSKLEESRRLWKFFWDMGEEEAWIREQSRLLLSDDYGKDLTSSLRLISKHNAFRGEMSGHRGPLQQTVAEGEELISKGHFGAGEIEERIKEVKGQWTEMEALAEDRERRLREAYSYYQFQADANDAEAWILDTLRLVSTSEVGHDEYSTQTLVKKHKEIEEEILSHRPAIDALHEQARGLPPAYSHSPEVDGRLPALEQRYEELVALAAHRKQALQDSLALHKMFSEADACLLWIDEKEQWLNVTEIPEKLEDLEVVQQRFETLEAEMNNLASRIASVNEVAGQLLTTDQRNENSIRDMQDQLNSRWNEFRKLVDQKKGALDSALDIQNYHLECNETVCWMREKTKVIESTQSLGNDLAGVTALQRKLTGMERDLKAIQGKVGDLRSEAEKLADGHPQKAPAIIGRLSDIDETWGELKDTMKRREETLGEASKLQGFLRDLDDFQSWLSRAQTAVASEDVPADLAEAERLLAQHEGIKNEIDHYGADYHRVRDVGEEVTRGQTDAQYMFLQQRLKALDTGWNELGQMWENRHQLLSQGHSFQQFLKDTKQVEGFLNNQEYVLSHTEMPSTLQAAEAAIKRHEDFLTTMDANEEKIQGLVESGRKLIEEQNVNSEKIQEKVTSIDERHKKNQKTASEVLTRLKDNRDLQHFLQDCQELTLWINEKMLTAQDVSYDEARNLHSKWQKHQAFMAELASNKDWLDKIDKEGQQLMSEKPELEPVVREKLDTLHKLWNDLESTTQSKAQCLFDANRAELFTQSCSALESWLQNLESQLQSDHYGKDLTSVNILLNKQQMLENQMEVREKELEAIQSQAQALGQEDANIVEVDSKHKDVADKFSKLRAPLNKRRKLLLASKEGHQFNRDLEDEILWVKEKMPMAISTDHGKDLPTVQLLIKKNQTLQKEILGHQPRIDDILERGKSMIQDSSLDPQEIEERLGLLKSTWEELAAEGGKRHARLEESLAAQQFYADAAEAEAWMGEQELHMMSEEKAKDEIGAQAMLKKHLVMEQALDDYADNIHQLANSSRDMVSRGHPESDRITLRQGQVDKLYASLKDLAEERQGKLQEHHRLCQLKRDVEDLEHWISEREVVAASHELGQDYEHVTMLRDKFREFSRDTSNIGQERVDAVNLMADDLISSGHSENATVADWKDQLNEAWADLLEMIDTRSQMLAASYELHRFYQDAKETLGQIQDKQKQIPEETGRDLNTAEAMQRMHTAFEHDIQALSAQVKQVQEDASRLQKAYAGEKAADIHRHEQAVSEAWGELQNASGARRQLLLDTVEKFRFFRMVRDLMLWMDDINLLIDAQEKPRDVSSADLVIQNHLGIKAEVDARADSFTTCIDLGNSLLEKNHYASDKISEKLEQLQERRQETENKWKDKMAWLRVVHEVLMFGRDASVAEAWLASQEPIVRSSELGGNVDEVESLIKRHEAFQKAVSTWDERFVLLEKLTTLEEIEMRRKREEEERKKRPPTPPLPVEEPREEVVKTQVSQTIEGQTDPANILQERINGMQNSFQEQVSVNGLCTESDSPQPSSDTQRLEDSISENQGPEQSDVTNGARTEVITKDGSPTPSPQLVARPTFSPINLQYSTLPSSPTEPLASEQMEGMLHRKQEMDSHNKKAANRSWQNVYCALKRGSLGFYKDAKSAASGVSYHSEPSVQLNGAQCSVAHDYKKRKNVFKLSLLDGKEYLFQAKDEAEMSSWMRVINASLTSSPGDPSDHSPGIMKGMTRAMTMPPVSPNSGTEGSVTLRNKDGKEKDREKRFSFFKKK

Target ORF information:

RefSeq Version	XM_012962405.3
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis spectrin, beta, non-erythrocytic 2 (sptbn2), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012962405.3

ORF Insert Sequence:

ATGAGCAGTC CACTGTCACC GACAGATTAT GACAGCCTGG AAATCCAGCA GCAGTATAAT 
GACATCAACA ATCGATGGGA TCTGACAGAT GATGATTGGG ACAATGAGAA CAGTTCTGCA 
AGACTTTTTG AGAGGTCCCG GATCAAAGCA CTTGCAGAAA TGGATTTTGT CCCCCGATCT 
GGCTCCCCTC TCCTATCCCC TGCGGCTTTT GTAAATGCAG TGCAATACGC TAACGTGCTG 
GAAGGAAGGT TCAAGCAGCT GCAAGATGAA AGAGAAGCTG TGCAGAAGAA GACATTTACT 
AAATGGGTCA ACTCTCACTT GGCGAGGGTC ACCTGTAGAA TTTCTGATCT CTACAATGAT 
TTACGAGATG GACGGATGTT AATTCGACTG TTAGAAGTGC TATCTGGAGA GCAGCTGCCA 
AAACCCACAA AAGGTCGAAT GCGAATCCAC TGTTTGGAAA ATGTAGACAA AGCCCTTCAA 
TTTTTAAAAG AGCAGAAGGT ACATTTGGAG AACATGGGAT CACATGACAT TGTGGATGGA 
AACCATCGAT TAACCCTAGG GTTAATCTGG ACTATTATTT TAAGGTTTCA GATCCAAGAT 
ATCAGTGTAG AGACAGAAGA TAATAAAGAA AAGAAGTCTG CTAAAGATGC CTTACTGCTT 
TGGTGCCAGA TGAAGACTGC TGGGTATCCA AATGTCAATG TTCACAACTT TACCACCAGC 
TGGCGAGATG GACTTGCGTT TAATGCCATT GTACACAAGC ACAGACCAGA TATAATTGAC 
TTTGACACAC TGAAGAAGTC TAATGCACAC CACAACCTCC AGAATGCCTT CAATGTAGCT 
GAAAGAGAGT TAGGTCTTAC TAAACTTCTG GATCCTGAAG ATGTTAATGT GGATCAGCCT 
GATGAGAAAT CCATCATAAC CTATGTGGCC ACATTTTATC ACTATTTTTC CAAAATGAAA 
GCCCTAGCTG TGGAAGGAAA GCGTATTGGA AAGGTACTTG ACCAAGCTAT TGAAGCTGAT 
CAGTTAATTG AGAACTATGA AGGACTGGCA TCAGAACTCC TGCAATGGAT TGAACAGATG 
ATCATTACTC TCAATGACCG AAAACTGGCA AACTCTCTAA GTGGTGTTCA GAACCAGCTG 
CAGGCCTTCA ACACCTACAG GACTGTAGAA AAGCCGCCCA AGTTCACAGA AAAGGGCAAT 
TTGGAAGTTC TTCTCTTTAC CATACAGAGT AAAATGAGAG CTAATAATCA AAAAGTGTAT 
ACGCCCAGAG AAGGAAAGCT TATATCTGAT ATAAACAAGG CATGGGAGCG ACTAGAAAAG 
GCAGAGCATG AACGTGAGCT TGCCCTTCGC AATGAACTTA TACGCCAGGA AAAATTAGAG 
CAGTTGGCTG CACGATTTGA CCGTAAAGCA GCCATGAGGG AAACCTGGCT AAGTGAAAAT 
CAGAGACTTG TAGCTCAGGA TAACTTCGGT GTGGACTTAA CTGCGGTGGA GGCAGCGCTA 
CGGAAACATG AAGCTATTGA AACTGATATA TTGGCTTACA GTGAGCGCGT GCAAGCAGTG 
AATGCAGTGG CTGATGAGCT GGAAGCAGAG AATTACCATG ATGTCCGGAG GATTTTGGCT 
CGGAAGTGCA ATGTGATTCG TCTCTCTGAT TATTTAAGAG AGCTTGTCAC TGCCAGGAGG 
CAAAGGCTTC ATATGAACCT AGAATTGCAG AGGATGTTCC AGGATTTACA TTATCTCATG 
GACTGGACTG AAGATATGAA GGGCCGTCTG CAGTCAGAAG ATTATGGCAA GCATCTTCAT 
GGCGTGGAAG ACCTTCTCCA GACTCACACT CTTGTGGAGG CAGACATTGC AGTTCAGGCA 
GAACGTGTGA AATCAGTTAC AGCTGTAGCT CAGAAATTTA TGACAGAAGA TGAAGGCTAC 
AAGCCTTGCG ACCCAGAGCT AGTGCGCAAC CGGGTGCATC TCTTGGAACA GCGCTATGCA 
GAGCTGTGTA ATCTTTCTGC GGCTCGGAGA TCAAAGCTGG AAGAATCCCG CCGTCTCTGG 
AAATTTTTCT GGGACATGGG AGAGGAGGAA GCCTGGATCC GTGAGCAGAG TAGATTACTA 
CTTTCTGATG ACTATGGGAA AGACCTTACC AGCTCACTTA GGTTGATCAG CAAGCATAAT 
GCCTTCAGAG GTGAGATGAG TGGACACCGT GGGCCGTTGC AGCAGACTGT GGCTGAGGGT 
GAGGAACTAA TATCCAAAGG TCACTTTGGT GCAGGAGAGA TTGAGGAAAG GATAAAAGAA 
GTGAAAGGCC AGTGGACAGA GATGGAGGCC TTAGCTGAAG ACAGGGAAAG GAGGCTAAGA 
GAGGCCTACA GCTATTATCA GTTTCAAGCT GATGCCAATG ATGCTGAGGC TTGGATCCTG 
GACACTTTAC GCCTTGTTTC TACCAGTGAA GTAGGACATG ATGAGTATTC CACACAGACA 
CTGGTGAAAA AGCACAAAGA AATAGAAGAG GAGATCCTTA GCCACCGACC AGCTATTGAT 
GCGCTGCATG AACAGGCCCG TGGCCTTCCA CCTGCTTACT CTCATTCACC TGAAGTTGAT 
GGAAGGTTAC CAGCCCTGGA GCAGCGCTAT GAGGAATTGG TGGCTCTTGC TGCTCACCGC 
AAGCAGGCTC TGCAAGATTC TCTGGCTCTT CATAAAATGT TTAGTGAAGC TGATGCTTGC 
CTTTTGTGGA TTGATGAGAA AGAACAGTGG CTTAATGTCA CAGAGATTCC AGAAAAACTT 
GAGGACCTAG AAGTAGTTCA ACAAAGGTTT GAAACTCTAG AGGCAGAGAT GAATAATTTG 
GCATCCCGGA TTGCATCTGT AAATGAAGTT GCTGGACAGT TGCTGACTAC AGACCAGAGA 
AATGAAAACA GCATCCGAGA CATGCAAGAT CAACTTAATT CTCGGTGGAA TGAATTCCGC 
AAGCTTGTTG ACCAAAAGAA GGGTGCTTTG GATTCTGCTC TGGATATACA GAATTATCAT 
TTGGAGTGCA ATGAGACTGT GTGCTGGATG AGGGAGAAGA CAAAGGTTAT TGAATCCACA 
CAGAGCCTAG GGAATGATCT TGCAGGAGTA ACAGCCCTCC AGAGAAAACT TACTGGAATG 
GAGCGTGATC TGAAAGCCAT TCAGGGAAAA GTGGGTGACC TGCGTTCAGA GGCAGAAAAG 
CTTGCAGATG GGCACCCACA GAAAGCACCG GCCATAATTG GCCGCTTATC AGATATTGAT 
GAGACCTGGG GAGAACTAAA GGACACTATG AAAAGAAGAG AAGAAACTTT AGGTGAAGCA 
AGCAAGCTGC AGGGATTTTT GAGAGACCTT GATGATTTCC AGAGCTGGTT GTCCCGGGCC 
CAGACAGCAG TGGCATCTGA GGATGTTCCA GCTGACCTAG CAGAAGCAGA GCGCCTTCTT 
GCACAACATG AAGGCATAAA AAATGAGATT GATCATTATG GAGCTGACTA CCACAGAGTG 
AGAGATGTTG GTGAAGAAGT CACTCGTGGG CAAACAGATG CCCAATATAT GTTCTTGCAA 
CAGAGACTGA AGGCGCTAGA CACTGGCTGG AATGAGCTTG GACAGATGTG GGAAAACAGG 
CACCAGTTGT TGTCCCAGGG TCACAGCTTC CAGCAGTTTT TGAAAGATAC AAAACAAGTG 
GAGGGGTTCC TTAATAACCA GGAGTATGTC CTTTCCCATA CCGAAATGCC AAGTACGCTA 
CAGGCAGCTG AAGCTGCCAT CAAACGCCAT GAAGATTTCC TCACAACCAT GGATGCCAAT 
GAAGAGAAGA TACAGGGTCT TGTAGAATCT GGGAGGAAGC TAATTGAGGA ACAGAATGTC 
AATTCTGAGA AGATTCAAGA AAAAGTCACT TCTATTGATG AGAGACACAA AAAGAACCAA 
AAAACTGCTA GCGAAGTATT AACTCGTTTG AAGGACAACC GTGATCTTCA ACATTTCCTT 
CAAGACTGTC AAGAGCTCAC CCTCTGGATC AATGAAAAAA TGCTTACTGC TCAAGATGTT 
TCTTATGATG AAGCTCGAAA CCTGCATAGC AAGTGGCAAA AGCATCAGGC ATTCATGGCA 
GAACTGGCCT CAAATAAAGA CTGGCTTGAC AAAATAGACA AGGAAGGGCA GCAGCTCATG 
TCAGAAAAAC CGGAGCTTGA ACCAGTAGTG AGAGAAAAAC TAGACACTCT GCATAAGCTG 
TGGAATGACT TGGAATCTAC CACACAAAGT AAAGCTCAGT GTCTGTTTGA TGCCAACAGG 
GCAGAGTTAT TCACACAAAG CTGTTCTGCT CTGGAGAGTT GGCTTCAAAA CCTCGAGTCA 
CAACTACAGT CAGATCATTA TGGCAAAGAT CTGACCAGTG TCAACATCCT ATTAAATAAA 
CAGCAGATGC TGGAGAACCA AATGGAAGTG AGGGAGAAGG AGCTAGAAGC TATACAGTCC 
CAGGCACAGG CCCTTGGACA GGAGGATGCC AACATAGTGG AGGTTGACTC AAAGCATAAG 
GATGTAGCAG ATAAATTTTC CAAGCTGCGT GCTCCTCTAA ACAAGAGAAG AAAATTACTT 
CTAGCCTCCA AGGAAGGACA TCAGTTTAAC AGGGACCTGG AAGATGAAAT ACTTTGGGTA 
AAGGAGAAGA TGCCAATGGC AATCTCCACT GATCATGGAA AAGATCTCCC GACAGTCCAG 
CTTCTTATCA AAAAGAATCA GACACTCCAA AAGGAAATTT TGGGACATCA ACCACGGATT 
GATGACATCC TGGAGAGAGG CAAATCTATG ATCCAAGACA GCTCTCTGGA CCCTCAAGAG 
ATAGAAGAAA GGCTTGGGCT ACTGAAAAGC ACATGGGAAG AGCTGGCAGC AGAAGGAGGA 
AAGCGCCATG CCCGTTTAGA GGAATCTCTT GCTGCTCAGC AGTTTTATGC TGATGCGGCA 
GAAGCTGAGG CCTGGATGGG AGAGCAAGAG TTACATATGA TGTCTGAAGA AAAAGCCAAG 
GATGAGATTG GTGCCCAGGC AATGCTGAAG AAACACTTAG TAATGGAACA GGCTCTTGAT 
GACTATGCAG ACAACATCCA TCAGCTTGCC AATTCAAGCC GCGACATGGT CTCCAGAGGT 
CATCCAGAAA GTGATCGTAT TACCCTTCGT CAAGGGCAAG TGGACAAGCT ATATGCCAGT 
CTTAAAGACC TGGCAGAGGA GAGACAAGGA AAGCTACAAG AGCACCACAG ACTTTGCCAA 
CTCAAGAGAG ATGTCGAAGA CCTTGAGCAC TGGATCTCTG AACGTGAGGT GGTTGCAGCT 
TCTCATGAAC TTGGGCAAGA TTATGAGCAT GTAACGATGC TGAGAGACAA ATTCCGTGAG 
TTCTCCCGTG ACACCAGTAA CATTGGTCAA GAGCGGGTGG ATGCTGTCAA TCTGATGGCA 
GATGATTTAA TTTCATCTGG ACATTCGGAA AATGCTACTG TAGCAGATTG GAAAGACCAG 
CTAAATGAGG CATGGGCTGA TCTTCTTGAG ATGATTGACA CTCGAAGTCA GATGCTGGCA 
GCCTCATATG AACTTCACCG TTTCTACCAG GATGCAAAAG AAACCCTAGG TCAGATCCAA 
GACAAGCAGA AACAAATACC AGAGGAGACA GGCAGAGATC TGAATACAGC AGAAGCCATG 
CAGCGCATGC ACACCGCTTT TGAACATGAT ATCCAAGCCC TGAGTGCACA GGTGAAGCAA 
GTCCAGGAAG ATGCTTCTCG CCTCCAAAAA GCTTATGCTG GTGAAAAGGC AGCAGACATT 
CATCGCCATG AGCAGGCAGT ATCGGAAGCT TGGGGAGAGT TGCAGAATGC TAGTGGTGCC 
AGGCGGCAGC TTCTTCTTGA CACTGTGGAG AAATTCCGTT TCTTCAGAAT GGTGCGAGAT 
CTCATGTTAT GGATGGATGA TATAAACCTG CTAATTGATG CACAGGAGAA GCCAAGAGAT 
GTATCCTCAG CTGATTTAGT CATCCAAAAC CACCTAGGCA TCAAGGCAGA AGTGGATGCG 
CGAGCTGACA GCTTTACTAC ATGCATAGAT CTTGGCAACT CTCTCCTAGA GAAGAATCAT 
TATGCCTCTG ACAAGATTTC AGAGAAACTG GAGCAATTGC AGGAAAGAAG GCAGGAGACT 
GAAAACAAAT GGAAAGATAA AATGGCATGG CTGAGAGTTG TCCACGAAGT GCTGATGTTT 
GGAAGAGATG CAAGTGTAGC TGAGGCTTGG CTGGCCAGCC AGGAGCCCAT TGTCCGGTCG 
TCAGAACTTG GTGGCAATGT CGATGAAGTA GAATCCCTGA TAAAAAGACA CGAAGCCTTT 
CAGAAAGCAG TTTCTACATG GGATGAGAGA TTTGTTCTTC TTGAAAAACT AACCACGTTA 
GAAGAAATTG AAATGCGTCG GAAGCGGGAA GAGGAAGAAA GAAAGAAAAG ACCACCAACA 
CCACCTCTAC CAGTAGAAGA GCCTCGAGAG GAAGTTGTAA AAACCCAAGT TTCCCAGACT 
ATTGAAGGGC AGACTGATCC AGCCAATATA CTTCAAGAAA GGATAAATGG CATGCAGAAT 
TCATTCCAGG AGCAAGTATC TGTTAATGGA CTGTGCACAG AGTCTGATTC TCCACAGCCA 
TCGTCCGATA CACAGAGGTT AGAAGACAGC ATCTCTGAAA ATCAGGGTCC GGAACAGTCA 
GATGTAACCA ATGGAGCCAG GACTGAGGTT ATTACCAAAG ATGGAAGTCC AACACCTTCT 
CCACAACTTG TGGCTAGGCC AACCTTCTCC CCGATAAACC TCCAGTATTC TACTTTGCCA 
TCTAGCCCCA CTGAACCATT GGCATCTGAG CAAATGGAAG GAATGCTGCA CCGCAAGCAG 
GAAATGGATT CTCACAACAA GAAGGCTGCG AACAGGTCCT GGCAGAATGT TTACTGTGCA 
CTGAAAAGGG GCAGTCTGGG TTTCTACAAG GATGCTAAAA GTGCAGCCAG TGGCGTTTCT 
TATCATTCGG AGCCCTCCGT CCAACTTAAT GGAGCCCAGT GCAGTGTTGC TCATGACTAC 
AAGAAACGCA AGAATGTCTT CAAGCTAAGC CTTCTTGATG GCAAAGAATA TCTGTTCCAA 
GCTAAGGATG AGGCTGAAAT GAGTAGTTGG ATGCGAGTTA TAAACGCCTC CCTAACTTCC 
TCCCCGGGAG ACCCCTCAGA CCATTCACCG GGCATCATGA AGGGTATGAC CCGGGCCATG 
ACAATGCCAC CAGTATCACC CAACAGTGGT ACAGAGGGAT CAGTCACATT GCGCAACAAG 
GACGGGAAAG AAAAAGACCG GGAAAAGAGA TTCAGTTTCT TTAAGAAGAA GTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

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sptbn2 cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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