frem3 cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

Gene
Clones
gRNAs

Summary

Gene Symbol	frem3
Entrez Gene ID	100485452
Full Name	FRAS1 related extracellular matrix 3
General protein information	Preferred Names FRAS1 related extracellular matrix 3 Names FRAS1-related extracellular matrix protein 3
Gene Type	protein-coding
Organism	Xenopus tropicalis(tropical clawed frog)

mRNA and Protein(s)

mRNA	Protein	Name
XM_002940081.5	XP_002940127.3	FRAS1-related extracellular matrix protein 3
XM_002940081.4	XP_002940127.2	FRAS1-related extracellular matrix protein 3

Pathways from BioSystems

Homologies

Danio rerio (zebrafish)	frem3	NP_001131131.1
Xenopus tropicalis (tropical clawed frog)	frem2	XP_002940127.2

Gene Ontology

The following frem3 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the frem3 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OXa04586	XM_002940081.4 Latest version!	Xenopus tropicalis FRAS1 related extracellular matrix 3 (frem3), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
OXa49882	XM_002940081.5 Latest version!	Xenopus tropicalis FRAS1 related extracellular matrix 3 (frem3), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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2-4 Clone	40% OFF
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

frem3 ( XM_002940081.4 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_002940127.2 Xenopus tropicalis FRAS1 related extracellular matrix 3 (frem3), mRNA.

Price & Availability↑

CloneID	OXa04586
Clone ID Related Accession (Same CDS sequence)	XM_002940081.4
Accession Version	XM_002940081.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 9378bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1473177600000
Organism	Xenopus tropicalis(tropical clawed frog)
Product	FRAS1-related extracellular matrix protein 3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_002940081.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGGACCC ACAAACACCT ACCTACAAGC TTAGGCTGCT TCACCCTGAT GTTAGCCGCT 
CTCAGCTGGG CAGCTGCAGA TGGCGACTCC CGTTTGTCCG GCCCAGATAA CATTATAGTA 
ACCAACCGAA GGATCCGGGT GCCCTTGGGC AGATCTGTGT TCCTGGACCC AGTGCAAGAC 
CTAGCGATCC AGGTGCAGCC CGGAGACAGC TGTATCCTCA CAGTGTTGGC CAATGACCCT 
CTGTCTCAGC GCCCCGGCTT CTTAACCCCT AAGCGATTTC CGTGCGATTT CGGTGCTGGG 
GAGGTAGAAT ACACTCACCT GGGCGCCCGT AGCCCTGCCT CGGACAGAGT GAGGATGCAG 
CTGAAGTACG ACTCGCCCAC ACACACCTGG ATTGTGCCCT TTGTGATCGA AGTGGAGCTG 
ATCTTCCAGC AGCTGGAGAT AGTGACCCGC AACTTGCCGC TGTTGGTGGA TTCGCCGCGG 
GAAATCAGCC CCCCAGTTGA TAAGAAGGTG CTGGGTTTCT CCTACGATCC AACCAAGGAG 
TCGTGTAAGA TCAGCCCGCT GATTGGCACA GGGGGTCTGC CCAGGTACGG CCAACTGCTC 
AATGGCACTG CCGCCCCTCA GCGGGTCACT GACTGTCACA CCTTCATCAA CTCGGGGCTC 
CGCTACCAGC ACACCGCGCC CCAAAACTCG CCCAACAGAG ACTATGTGCC CATGCTGGCC 
CAGCTCAATG ATGGGCACGG AGTAGCCAGG CAGGAACACT TCCAGCTCAC CGTTATGATC 
AAGGGGGGAG CGGAGAACAC AGCCCCCCGG CCCAGCTTTG TGTCTATGAT GATGATGGAA 
GTGGGTCAGT TCGTGCTGAC AGCCCTCACC CCGGAGATGT TGGCGGCTGA GGATGCGGAG 
TCGGAACCGG GCGAGCTTAT CTTTAACGTG ACTAGCTCTC TGGATCCGGA GACATCGGGC 
TACCTGATCA GCACCGACGA TCGCAACCTG CCCATTACTT CTTTCTACCA GCGGGACCTG 
ATGGAGCTGA GGATCGCGTA CCAGCCCCCG GCTAGTGAGT CCTGGCAGGA GCGGATCATT 
CAGCTGGAGA TGCATGTCAT TGACACCGAG GGGGCATCGT CCGATTACTT TGCGTTTATG 
ATCGTTGTAA AGCCCATGAA CACACTGGCC CCACTGGCGA CTCGCAACGC TGGCCTGCGG 
CTCCTAGAGG GTCAGTCTCG CCCTTTGTCC GGCCCAGGCT CCGGCATGAA CAACCTCGTT 
ATAAGCGATG AGGACAACCT GGACCAAGTC AGGATCAGTG TGATCGATGG TCTCCGGCAC 
GGCCAGCTAA TCCTAAGCCC CAGTGACAGC GGGACCAATA GTGGCCACAA GGACTTCTCC 
TTTAGCCCAC AGGAACTTCA CTCGGGGCTG GTCATTTATC AGCACGATGG CAGCGACACC 
TACAGCGACA ATCTGATCCT AAGGCTGGAC GATGGCAAGC ATCGGGTGGA GCTGCTGTAC 
CCCATCTCAG TGGCCCCTAC TGATGATGAG CCCCCAGTAC TGAATGCCAA CACCGGGCTG 
AACCTACGGG AGAAGGGCCT GGCTCTCATC TCCCCTTTCA TGCTCAGTGC CACAGACATC 
GACTCGGAAG ATTCCACCAT ACACTTTGTG CTGCAGGAAC CCCTCGGCCG ACTCGGGGAG 
ATGCTGCTGA GGCAGCCGGA TGAGCCGGAG GGGGAGGAGG GCTGGAAATA TCTGCCCCAG 
GAAGGGATTT ACGAGAAGGG GGTGATCGAG TGGTACCAGA AGGATATTCT GGATGGGAAA 
CTGCACTACA GGCACCTGGG TTCGCATAGT ACAACTACAG TTCTGGACCG AGTGCTGTTC 
GTGGTGCTGG ATGACCATGA CCCCCCAAAC CAGTCCGATG AGCAGGTATT TATGGTGCGC 
ATAGAGCCCC TTGATGACAT CCCCCCAGAA CTGTACCCCG GTACTACCCT GCACATGACT 
GTCAAGGAAT ACGAGGTGAC CCCCTTCAGG AAGAAGTACC TGCGCTACAC TGATCTGGAC 
ACAGACGACC GAGAGCTTAA ATATACCTTG CACAGTCACC CTAGGGATAC CGACCCCAAC 
CATCCTGCCC TGACTGGCCA GATTGTGCTG AGTGACAGCC TGGGGGTCGA GGTGAACCAA 
TTTACACAAG CCCAGATCAA CCATTTAAAG ATCTCATACA AGCCCCCAGA GAAAGAACTG 
GGCATAGTTC CGAGGGAGCT ACAGTTCAAC TACACAGTGG AGGATGCTGA GGGAAATTCG 
GTGCAAGGGA CATTCACTAT AGTGTTACAG CCAGTGGACA ATAAACCTCC GGAGATCACA 
AATACTGGTT TCACTGTCCC AGAGAGGGGC TCCTTCACTC TCACCAAAAA TGAACTGGAT 
GCCACAGACC AAGACACAGA CACAAGCAAC ATCATTTTCA GCTTGTCTGA AGCCCCTCAG 
CATGGAGAGT TACAATACCA GGGTGTACCC ATGTCATTGG GAGAATCCTT TGCTTTAAGT 
GACATTTCAC TGGGTTGGGT ATCCTATGTT CATAGTGGAG AGGAGACCAC CAGCGACACA 
TGTTCCCTGG TAGTTAGTGA TGGTGTTCAC GAAGTTCCCA TTACAGTCCG AATTAAAGTA 
AAGCCAGTGG ATGACGAGGC ACCCATTTTG ATGCTTGCTG CAGGTCTTTT GGGGTCATCC 
ATTGAAGTCT TGGAGAGAGG AGAAACTGAG ATTACCAGCA GTGTTGTTCA GGGCACAGAT 
CCAGACACTG ACGATTTGAT GCTCACATTC ATTGTGGAGG AGCCTCCCAG AAAAGGGGAT 
ATATTGGTCA ATGGAGTTAC CGCTGAGAGG TTCTCTCAGC AAGACCTTCT CAATGGTGCT 
GTTACATATG TACATACATT TGGAGAGATT GGGCTTCAGA AGCAACATGA TTCCTTCAAT 
CTCACCCTCT CTGACCTCTC TGATGAGTGG GTGGTGGGAG GAAACAAGGT GCAGGGGGTG 
AGGGTGCAAG TAACCATCTT ACCAGTAGAC AGTGAAGCTC CAGTAGTAAC ATTAAAAGAA 
AAGTTCTCTG TTTTGGAAGG TGAGAAAAAT GTCATCACCT ATGAAAACCT GGACGCAGAT 
GACAAGGACA CCCCTAAAGA TGACATCTTG TGCACTATTA TTGTCCAGCC TACTTCTGGC 
TATGTAGAGA ACATTTCACC TGCCCCTGGC TCTGAAAAGT CAAGGTCTGG GACAGCAGTC 
AGTGCATTTA CAATAAGAGA TATCCGACTG GGTCACATTT ACTATGTTCA AAGCATCCAT 
CAGGGTGTAG AACCTCAGGA AGATAGATTC ACTTTTCACT GCTCTGATGG AATAAGCTCT 
TCACAGCGCC TTTTTTTCCC CATAGTGATC ATCCCAACCA ATGATGAGAA ACCAGAACTT 
TTCACACGGG AATTTGTAGT CATGGAAGGC ATGAGCCTGG TGATTGACAC CCCAATCCTT 
AATGCTGCAG ATGGAGACCA ACCACCGGAT GAACTTACCT TTATAATATC CAAGCCACCA 
CTACACGGTA GGGTGGTCCA ACAGCAAGCA ACAGGCACTG TCCCCATTCT GAATTTTACT 
TTAGAAGAAA TCAAAGAGAG CTCAAGCATT GTATATGAGC ATGACGATTC AGAAACCAAG 
GAGGACAGCT TTGAGATTAT CTTAACTGAT GGAGAACACA ATGTAATAAA GAAAGTCCTT 
ATTATGGTTA TTCCCGTGGA TGATGAGACT CCAAGAATGG CCATCAATGA TGGACTTGAA 
ATTGATATTG GAGAGTCAAA GGTAATCACA AACAGGGTTT TAAAGGCTAC AGACTTAGAT 
TCTGATGACA AGTCATTGGC TTATGTTATT CGGTTTGGCC CCAGCCAAGG TTTTCTGCAG 
CGCTTGGACA AACATGGGTC AGTGGTTGGT AATATTACCT TGGGAATGAA TTTTACTCAA 
GAGGACATTG ACAGAGGTTT AATTCGGTAC ACCCATGCTG GTCAGGAAGG CATACGGGAC 
CTTTTTAAAT TTGACATAAC AGATGGGATT AACCCATTCA TAGACAGATA CTTTTATATA 
AGCATTGGCA GCATTGATAT TGTTTTCCCA GATGTCATAA ACAAAGGAGT TACTCTCAAA 
GAAGGCGGAA AGGTGACACT TACTACAGAC CTTCTAAGTA CAAGTGACAT TAACAGTCCA 
GATGAAAATC TGCGCTTCAG TATTACAAGG GCCCCAAGCC GTGGGCACTT AGAGAGCACT 
GATGCCCCTG GTGTTCCCAT TACATCTTTT ACTCAGCTCC AACTAGCTGG GAATAAGGTC 
TATTACATCC ACACTTCAGA GGATGAGATG AAGATAGACA GTTTTGAGTT TGAAGTGACA 
GATGGTTATA ACCCAGTTTT CCGTACTTTT AGGGTCTCTA TTACTGATGT AGATAATAAA 
AAGCCAGTCT TGATGATAAA TGGACTAGTG GTCAATGAAG GAGAAAATAA GCTTATAACT 
CCCTTTGAGC TAAGTGTGGA GGACAGGGAC ACCCCAGATA ATTTGCTGCG ATTTACAATC 
ACTCAAGTGC CTGTTCATGG TCAGATTGTG TTCAACAACA GCCAGACAGT TACTACTTTC 
ACCAAGCAAG ACCTGAATGA GAATCTCATT AGCTACAAGC ATGATGGCAG TGAGTCTGTA 
GAAGATAGCT TTTCCTTCAC AGTCACTGAT GGGACCCATG CAGAGTTTTT TGTACTGCCA 
GACACAACCT TTGAAAGCAA GAAACCCCAA ATGATGAAGA TCAGAATAAA TTCTTTGGAC 
AATGGTGTAC CCCAACTTAT TGTCAACAAG GGGGCCCCTA CTCTTAGAAG CCTACATCCT 
GGCCAACTTG GGTTCACGAT CACCAGCAAA TCCCTAAAGG CAGAAGATCG GGACAGCCCT 
CACAAGATGC TCAAATACAG AATCCTAGAT GGACCTAAAC ATGGGTTTTT AGTCAACTTG 
GACCATGGCA ATGACAGCAT CAGAACCTTC ACACAGGGCG ACATTGATGA CATGAAAATC 
TGCTATGTTC TGAAGGATGG TGACAATGCT ACAGGAGATA TTTTCCATTT CTCAGTAGAA 
GATATAGGGG GCAATAAACT AAGGAGTCAG CAATTTCGCC TGAACTGGGC TTGGATTTCA 
ATGGAAAAAA GCCATTATAC TGTAGACGAA GATTCAAAGT TTCTGGAAGT GACACTTAAA 
CGAAGAGGAT ACCTTGGAGA GACATCTTTT ATCAGCATCA CTACAAAAGA TGGCACAGCA 
ACCAAGGATA AAGACTTTAA AGGAAAAGCC CAGAAGCAGG TTCAGTTTAA CCCCGGGCAG 
ACTACAGCCA CATGGAAAGT GCGAATTCTT GCAGATAATG AATATGAGAA TTCTGAGACA 
TTTCATGTTA TCTTATCAGA ACCTGTTATG GCTGCCCTTG AGTTTCCAAT AGAAGCAACA 
ATTGAAATTG TAGATCCAGG TGATGAGTCC ACTGTTTACA TACCGCAAGC AGAATACAAG 
ATAGAGGAAG ATATTGGAGA ACTGATAATC CCTATTAAAA GATCAGGCGA CATAAGTCAA 
GAACTTATGG TAGTTTGTTC AACGCATCAA GGTACAGCTA CAGGCACTGT TCCCTCCTCT 
GTATTGTCCT ACTCTGACTA CATCACACGT GCAGATGGAC ACACAAGTAT TATTCGCTTT 
GACAAAGATG AGACTGAGAA AAACTGCCGT GTTATTATCA TTGATGACTC CCTGTATGAA 
GGAGACGAGA CATTTAATGT CAGCCTTAGT ATGCCCATGG GAGGACAGCT GGGAGAATTT 
CCAAGTACTA AAGTGGTTAT CCTTGCTGAT ACAGATGATG AGCCAGCCTT CTACTTTGCA 
GATACAGATT ATCATGTTGA TGAGAGTGCT GGGTATGTTG AAGTTCGTGT TTGGAGAACA 
GGGACAGATC TGACCAAAGC AGCCACGGTC ACTGTGCGTT CCAGGAAAAG CGAGCCTCTG 
TCTGCAGAAG CTGGAACAGA TTATGTGGGT ATCAGCAGAA ACCTTGATTT TGCCCCTGGT 
GTAACCATGC AGAGCTTCCG AGTAGTAATT CTTGATGATC TGGGGCAGCC TTCTCTGGAG 
GGACTTGAGA AATTTGAATT GGTTCTTCAA ATGCCCATGG GAGCAGTATT GGGGGAGCCC 
AGCAAAACAA CTGTGACCAT CAATGATACC ATCTCTGACT TACCTAAGGT TCAGTTTAAG 
GAGTCAGCTT ATTATGCCAA CGAGAAAGAT GGGCAAGTAT CTGCCGTTAT ATACCGCAGT 
GGTGATGTCA GCCATAAATC CTCAGTGCGT TGCTTCACCC GCCAAGGATC GGCGCAAGTC 
ACAATGGATT ATAACGAAAG GCCAAACACT GATGATTCTG CTGTCGTCTT CTTGCCTGGT 
GAGACTGAGA AGGCATGTGT GGTAACCCTG GAAGATGATA AAGTATATGA AGAAGAAGAA 
GAGTTCCGAT TGCTACTTGG AACACCCAAA AGTGACTCAC CTTTTGGAGC CTCAGTTGGA 
GAGTCTAAAG AAGCTCTCAT AAAGATTAAA GATGCGGAGG ACAAACCAAT TATCAGGTTT 
TCAGAAATCA AGTATAGTAT CCAGGAGCCC CAGGAGTCCG GTGAAATAGC TGTTGTGAAA 
ATCCCTGTGC TGCGAATTGG AGACAACTCC AAGGTGTCCA TTGTTAGAAT TCACACCAAA 
GATGGCTCTG CTACATCAGG AGAAGATTAT AACCCATTGT CTGAAGATAT TGAGTTTAAA 
GAAGGAGAAA CCGAACACTT TGTTGAAGTG GAGATATTAT ATGATGGAGT AAGAGAAATG 
CGTGAAGCAT TTACTATACA CCTGAAGCCT GATGAGAACA TGGTAGCTGA AATCCAGATG 
AATAAAGCCA TAGTGTATAT TGAAGAGATG GACAGTGTTG CTGATGTGAC TTTTCCAGCT 
GTACCACAGG TTGTCTCACT TTTGCTGTAT GATGACACCT TCAAAATCAA GGAAAATCCC 
AGTCCCCCCA CTGGTTACCC AGTTGTATGT GTAACGGCGT GTAATCCAAA ATACTATGAC 
TATGACAAAA CTGGATCAAT CTGTGCTGCT GAAAACATTA ATGATACTCT GACCCAGTAC 
CGCTGGCTCG TCAGCGCTCC AAGTGGAAGT GATGGTGTCA CCAGCCCCAT GCGTGAAGTT 
GACTCCAATA CGTTCTTCAC TGACACTAAG TCGATCACAT TGGACTCTGT CTACTTCCAT 
GCTGGATCAA GAATCCAATG TGCAGCCAGG GCAGTCAATG CAAATGGAGA CTCTGGTCTG 
GAATTGCTAA GCCCTATATT TAGTGTTAGC AGAGAGGAAG GTCTTTGTCA GCCTCGTATT 
CCTGGAACAG TGGGTGCTGA ACCATTCTCA GCAAAAATCC GTTACACAGG CCCTGAAGAT 
CCTGACTACC CTAATCTGAT AAAACTGACA GTAACAATGC CACATATGGA CGGTATGCTT 
CCAGTCATCT CAACCAGACA CATGTCCAAC TTTGAATTGA CGCTTAGTCC AGATGGCACA 
AGAGTTGGAA ACCACAAGTG CTCCAACCTT CTAGATTACA ATGAGATTCC AACCAAGCAC 
AGCTTTATTA CAGACAATAC CAAGAGCCCT GAAGTGATCG GAGAAACATC CCCCTACCAG 
TACAGCAATG CTCTTCGATC TGCTAGAACT CTACGCTTCT ATCGCAACCT CAATTTGGAG 
GCCTGTCTGT GGGAATTTAA CAGTTACTAT GACATGTCTG AACTTCTGAC AGATTGTGGC 
GGAACTATAG GGACAGATGG ACAAGTGCTG AATCTGGTCC AGTCCTATGT AACTCTACGG 
GTTCCCCTCT ATGTATCCTA TGTGTTTCAC TCGGCAGCAG CAGTTGGTGG GTGGCAGCAC 
TTTGACCTGC AGTCAGAGTT AAGGTTGACA TTTGTGTATG ATACTGCCAT CTTATGGAAC 
GAAGGAATAG GCAGCCCACC AGAATCTGAG CTACAGGGAT CCCTTTATCC AACGAGCATG 
CGCATTAATG ATGAAGGACG CTTGGTAGTC AATTTTAAGA CAGAGATTCG CTTTCGGGGA 
CAGTTTGTCA TTTCTCATCC AGGTACATCT CTTTCATCCA TGGTGATGTC TGCCGACCAT 
CCTGAACTCA CCTTTACATT GAGTCTGTTG CGCAGTGAGC CAAGCTTCAA TCAACCCGTT 
CAGCAATGGA GCTTCATCTC CGACTTTGCT GTACGTGATT ATTCTGGCAC ATATACAGTG 
AAGCTTATCC CATGTAGCAC CACCCCAAAC CAAGAATATT CTTTACCTGT TATCTGCAAT 
CCACGTGAAC CTATATCCTT CAATATCGAC ATTAGGTTCC AACAGGTTAG TGACCCAGTG 
GCAGCAGAAT TCAGTCTTAA CACCCAAATG TTGTTGCTGT CTAAAAAAGA ATTGTGGTTA 
TCAGATGGCT CAATGGGATT TGGCCAGGGA AGTGATGTTG CATTTACTGA AGGCTCTACA 
ATCTATGGGC GAATAATGGT GGATCCAGTT CAGAACCTTG GGGACTCTTT TATCTGCAAC 
ATTGAGAAAG TGTTTTTATG CACTGGTTCT GATGGTTATG TCCCCAAATA CAGCCCAGAA 
AACAAGGAAT ATGGATGCTT TGCGGACTCG CCAACATTAT TGTACAGATT TAAAATACTG 
GACAAAGCAC AGCCAGAAAC ACAGGCCAGG TTATTTGGAG ATATTCCATT CGAAGCCAGA 
TTAGCTATTG ATAACCCCAG TGCTCTGGCG CTGGTGAAGC AACCAGGTTC TGATGGTTTC 
AGCCTCTCAT CTGCGCCACT GTTTCAGGTG GCTTCTGGAC GGGAGTGGTT TATTCATACT 
ATTTACACAG TCCGCTCTAA GGAGAATGCA AACCGAGGGA TTGGAAAGCG TAGTGTGGAT 
TACCATCATG CCTTGAGTAG CGCTACTGAC CCCAGGGGGG AACTCACACT AAAGAGAAGC 
CGGAGAGCAT ATCCAGAAGA TTCCGCATTT GCACAGGACA TTGGTGCTGA TAAGAACAGA 
GGGACCAACA TTCAGCACAT TGCTTTGGAG CAGAGCAGCA AGGCCTCAGG TTACAATAAG 
GACATGTTGC CTGGTGGTGG CCAATATCAG AAGCCAGTCT TGGAAGTAAC AGGCCACAAC 
TCAGAGGGAC CTTTGGTGCC TGTTCTTGGA GGGGTGGGTG CTTTATTGCT CTTAATCTGC 
ACTGTGGTTA TCATTGGTCT GCTCGTGAAA CACCGGAGAC GGCAGCACTC TGAGAAGAAG 
TCTCCAACTA ATAGTTATCA TAACAGCCAT GAAACTATGA GTACAAGGCA CAGCAGCAGT 
GACAGCTCGG AAGTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_002940127.2
CDS	373..9750
Translation	MGTHKHLPTSLGCFTLMLAALSWAAADGDSRLSGPDNIIVTNRRIRVPLGRSVFLDPVQDLAIQVQPGDSCILTVLANDPLSQRPGFLTPKRFPCDFGAGEVEYTHLGARSPASDRVRMQLKYDSPTHTWIVPFVIEVELIFQQLEIVTRNLPLLVDSPREISPPVDKKVLGFSYDPTKESCKISPLIGTGGLPRYGQLLNGTAAPQRVTDCHTFINSGLRYQHTAPQNSPNRDYVPMLAQLNDGHGVARQEHFQLTVMIKGGAENTAPRPSFVSMMMMEVGQFVLTALTPEMLAAEDAESEPGELIFNVTSSLDPETSGYLISTDDRNLPITSFYQRDLMELRIAYQPPASESWQERIIQLEMHVIDTEGASSDYFAFMIVVKPMNTLAPLATRNAGLRLLEGQSRPLSGPGSGMNNLVISDEDNLDQVRISVIDGLRHGQLILSPSDSGTNSGHKDFSFSPQELHSGLVIYQHDGSDTYSDNLILRLDDGKHRVELLYPISVAPTDDEPPVLNANTGLNLREKGLALISPFMLSATDIDSEDSTIHFVLQEPLGRLGEMLLRQPDEPEGEEGWKYLPQEGIYEKGVIEWYQKDILDGKLHYRHLGSHSTTTVLDRVLFVVLDDHDPPNQSDEQVFMVRIEPLDDIPPELYPGTTLHMTVKEYEVTPFRKKYLRYTDLDTDDRELKYTLHSHPRDTDPNHPALTGQIVLSDSLGVEVNQFTQAQINHLKISYKPPEKELGIVPRELQFNYTVEDAEGNSVQGTFTIVLQPVDNKPPEITNTGFTVPERGSFTLTKNELDATDQDTDTSNIIFSLSEAPQHGELQYQGVPMSLGESFALSDISLGWVSYVHSGEETTSDTCSLVVSDGVHEVPITVRIKVKPVDDEAPILMLAAGLLGSSIEVLERGETEITSSVVQGTDPDTDDLMLTFIVEEPPRKGDILVNGVTAERFSQQDLLNGAVTYVHTFGEIGLQKQHDSFNLTLSDLSDEWVVGGNKVQGVRVQVTILPVDSEAPVVTLKEKFSVLEGEKNVITYENLDADDKDTPKDDILCTIIVQPTSGYVENISPAPGSEKSRSGTAVSAFTIRDIRLGHIYYVQSIHQGVEPQEDRFTFHCSDGISSSQRLFFPIVIIPTNDEKPELFTREFVVMEGMSLVIDTPILNAADGDQPPDELTFIISKPPLHGRVVQQQATGTVPILNFTLEEIKESSSIVYEHDDSETKEDSFEIILTDGEHNVIKKVLIMVIPVDDETPRMAINDGLEIDIGESKVITNRVLKATDLDSDDKSLAYVIRFGPSQGFLQRLDKHGSVVGNITLGMNFTQEDIDRGLIRYTHAGQEGIRDLFKFDITDGINPFIDRYFYISIGSIDIVFPDVINKGVTLKEGGKVTLTTDLLSTSDINSPDENLRFSITRAPSRGHLESTDAPGVPITSFTQLQLAGNKVYYIHTSEDEMKIDSFEFEVTDGYNPVFRTFRVSITDVDNKKPVLMINGLVVNEGENKLITPFELSVEDRDTPDNLLRFTITQVPVHGQIVFNNSQTVTTFTKQDLNENLISYKHDGSESVEDSFSFTVTDGTHAEFFVLPDTTFESKKPQMMKIRINSLDNGVPQLIVNKGAPTLRSLHPGQLGFTITSKSLKAEDRDSPHKMLKYRILDGPKHGFLVNLDHGNDSIRTFTQGDIDDMKICYVLKDGDNATGDIFHFSVEDIGGNKLRSQQFRLNWAWISMEKSHYTVDEDSKFLEVTLKRRGYLGETSFISITTKDGTATKDKDFKGKAQKQVQFNPGQTTATWKVRILADNEYENSETFHVILSEPVMAALEFPIEATIEIVDPGDESTVYIPQAEYKIEEDIGELIIPIKRSGDISQELMVVCSTHQGTATGTVPSSVLSYSDYITRADGHTSIIRFDKDETEKNCRVIIIDDSLYEGDETFNVSLSMPMGGQLGEFPSTKVVILADTDDEPAFYFADTDYHVDESAGYVEVRVWRTGTDLTKAATVTVRSRKSEPLSAEAGTDYVGISRNLDFAPGVTMQSFRVVILDDLGQPSLEGLEKFELVLQMPMGAVLGEPSKTTVTINDTISDLPKVQFKESAYYANEKDGQVSAVIYRSGDVSHKSSVRCFTRQGSAQVTMDYNERPNTDDSAVVFLPGETEKACVVTLEDDKVYEEEEEFRLLLGTPKSDSPFGASVGESKEALIKIKDAEDKPIIRFSEIKYSIQEPQESGEIAVVKIPVLRIGDNSKVSIVRIHTKDGSATSGEDYNPLSEDIEFKEGETEHFVEVEILYDGVREMREAFTIHLKPDENMVAEIQMNKAIVYIEEMDSVADVTFPAVPQVVSLLLYDDTFKIKENPSPPTGYPVVCVTACNPKYYDYDKTGSICAAENINDTLTQYRWLVSAPSGSDGVTSPMREVDSNTFFTDTKSITLDSVYFHAGSRIQCAARAVNANGDSGLELLSPIFSVSREEGLCQPRIPGTVGAEPFSAKIRYTGPEDPDYPNLIKLTVTMPHMDGMLPVISTRHMSNFELTLSPDGTRVGNHKCSNLLDYNEIPTKHSFITDNTKSPEVIGETSPYQYSNALRSARTLRFYRNLNLEACLWEFNSYYDMSELLTDCGGTIGTDGQVLNLVQSYVTLRVPLYVSYVFHSAAAVGGWQHFDLQSELRLTFVYDTAILWNEGIGSPPESELQGSLYPTSMRINDEGRLVVNFKTEIRFRGQFVISHPGTSLSSMVMSADHPELTFTLSLLRSEPSFNQPVQQWSFISDFAVRDYSGTYTVKLIPCSTTPNQEYSLPVICNPREPISFNIDIRFQQVSDPVAAEFSLNTQMLLLSKKELWLSDGSMGFGQGSDVAFTEGSTIYGRIMVDPVQNLGDSFICNIEKVFLCTGSDGYVPKYSPENKEYGCFADSPTLLYRFKILDKAQPETQARLFGDIPFEARLAIDNPSALALVKQPGSDGFSLSSAPLFQVASGREWFIHTIYTVRSKENANRGIGKRSVDYHHALSSATDPRGELTLKRSRRAYPEDSAFAQDIGADKNRGTNIQHIALEQSSKASGYNKDMLPGGGQYQKPVLEVTGHNSEGPLVPVLGGVGALLLLICTVVIIGLLVKHRRRQHSEKKSPTNSYHNSHETMSTRHSSSDSSEV

Target ORF information:

RefSeq Version	XM_002940081.4
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis FRAS1 related extracellular matrix 3 (frem3), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_002940081.4

ORF Insert Sequence:

ATGGGGACCC ACAAACACCT ACCTACAAGC TTAGGCTGCT TCACCCTGAT GTTAGCCGCT 
CTCAGCTGGG CAGCTGCAGA TGGCGACTCC CGTTTGTCCG GCCCAGATAA CATTATAGTA 
ACCAACCGAA GGATCCGGGT GCCCTTGGGC AGATCTGTGT TCCTGGACCC AGTGCAAGAC 
CTAGCGATCC AGGTGCAGCC CGGAGACAGC TGTATCCTCA CAGTGTTGGC CAATGACCCT 
CTGTCTCAGC GCCCCGGCTT CTTAACCCCT AAGCGATTTC CGTGCGATTT CGGTGCTGGG 
GAGGTAGAAT ACACTCACCT GGGCGCCCGT AGCCCTGCCT CGGACAGAGT GAGGATGCAG 
CTGAAGTACG ACTCGCCCAC ACACACCTGG ATTGTGCCCT TTGTGATCGA AGTGGAGCTG 
ATCTTCCAGC AGCTGGAGAT AGTGACCCGC AACTTGCCGC TGTTGGTGGA TTCGCCGCGG 
GAAATCAGCC CCCCAGTTGA TAAGAAGGTG CTGGGTTTCT CCTACGATCC AACCAAGGAG 
TCGTGTAAGA TCAGCCCGCT GATTGGCACA GGGGGTCTGC CCAGGTACGG CCAACTGCTC 
AATGGCACTG CCGCCCCTCA GCGGGTCACT GACTGTCACA CCTTCATCAA CTCGGGGCTC 
CGCTACCAGC ACACCGCGCC CCAAAACTCG CCCAACAGAG ACTATGTGCC CATGCTGGCC 
CAGCTCAATG ATGGGCACGG AGTAGCCAGG CAGGAACACT TCCAGCTCAC CGTTATGATC 
AAGGGGGGAG CGGAGAACAC AGCCCCCCGG CCCAGCTTTG TGTCTATGAT GATGATGGAA 
GTGGGTCAGT TCGTGCTGAC AGCCCTCACC CCGGAGATGT TGGCGGCTGA GGATGCGGAG 
TCGGAACCGG GCGAGCTTAT CTTTAACGTG ACTAGCTCTC TGGATCCGGA GACATCGGGC 
TACCTGATCA GCACCGACGA TCGCAACCTG CCCATTACTT CTTTCTACCA GCGGGACCTG 
ATGGAGCTGA GGATCGCGTA CCAGCCCCCG GCTAGTGAGT CCTGGCAGGA GCGGATCATT 
CAGCTGGAGA TGCATGTCAT TGACACCGAG GGGGCATCGT CCGATTACTT TGCGTTTATG 
ATCGTTGTAA AGCCCATGAA CACACTGGCC CCACTGGCGA CTCGCAACGC TGGCCTGCGG 
CTCCTAGAGG GTCAGTCTCG CCCTTTGTCC GGCCCAGGCT CCGGCATGAA CAACCTCGTT 
ATAAGCGATG AGGACAACCT GGACCAAGTC AGGATCAGTG TGATCGATGG TCTCCGGCAC 
GGCCAGCTAA TCCTAAGCCC CAGTGACAGC GGGACCAATA GTGGCCACAA GGACTTCTCC 
TTTAGCCCAC AGGAACTTCA CTCGGGGCTG GTCATTTATC AGCACGATGG CAGCGACACC 
TACAGCGACA ATCTGATCCT AAGGCTGGAC GATGGCAAGC ATCGGGTGGA GCTGCTGTAC 
CCCATCTCAG TGGCCCCTAC TGATGATGAG CCCCCAGTAC TGAATGCCAA CACCGGGCTG 
AACCTACGGG AGAAGGGCCT GGCTCTCATC TCCCCTTTCA TGCTCAGTGC CACAGACATC 
GACTCGGAAG ATTCCACCAT ACACTTTGTG CTGCAGGAAC CCCTCGGCCG ACTCGGGGAG 
ATGCTGCTGA GGCAGCCGGA TGAGCCGGAG GGGGAGGAGG GCTGGAAATA TCTGCCCCAG 
GAAGGGATTT ACGAGAAGGG GGTGATCGAG TGGTACCAGA AGGATATTCT GGATGGGAAA 
CTGCACTACA GGCACCTGGG TTCGCATAGT ACAACTACAG TTCTGGACCG AGTGCTGTTC 
GTGGTGCTGG ATGACCATGA CCCCCCAAAC CAGTCCGATG AGCAGGTATT TATGGTGCGC 
ATAGAGCCCC TTGATGACAT CCCCCCAGAA CTGTACCCCG GTACTACCCT GCACATGACT 
GTCAAGGAAT ACGAGGTGAC CCCCTTCAGG AAGAAGTACC TGCGCTACAC TGATCTGGAC 
ACAGACGACC GAGAGCTTAA ATATACCTTG CACAGTCACC CTAGGGATAC CGACCCCAAC 
CATCCTGCCC TGACTGGCCA GATTGTGCTG AGTGACAGCC TGGGGGTCGA GGTGAACCAA 
TTTACACAAG CCCAGATCAA CCATTTAAAG ATCTCATACA AGCCCCCAGA GAAAGAACTG 
GGCATAGTTC CGAGGGAGCT ACAGTTCAAC TACACAGTGG AGGATGCTGA GGGAAATTCG 
GTGCAAGGGA CATTCACTAT AGTGTTACAG CCAGTGGACA ATAAACCTCC GGAGATCACA 
AATACTGGTT TCACTGTCCC AGAGAGGGGC TCCTTCACTC TCACCAAAAA TGAACTGGAT 
GCCACAGACC AAGACACAGA CACAAGCAAC ATCATTTTCA GCTTGTCTGA AGCCCCTCAG 
CATGGAGAGT TACAATACCA GGGTGTACCC ATGTCATTGG GAGAATCCTT TGCTTTAAGT 
GACATTTCAC TGGGTTGGGT ATCCTATGTT CATAGTGGAG AGGAGACCAC CAGCGACACA 
TGTTCCCTGG TAGTTAGTGA TGGTGTTCAC GAAGTTCCCA TTACAGTCCG AATTAAAGTA 
AAGCCAGTGG ATGACGAGGC ACCCATTTTG ATGCTTGCTG CAGGTCTTTT GGGGTCATCC 
ATTGAAGTCT TGGAGAGAGG AGAAACTGAG ATTACCAGCA GTGTTGTTCA GGGCACAGAT 
CCAGACACTG ACGATTTGAT GCTCACATTC ATTGTGGAGG AGCCTCCCAG AAAAGGGGAT 
ATATTGGTCA ATGGAGTTAC CGCTGAGAGG TTCTCTCAGC AAGACCTTCT CAATGGTGCT 
GTTACATATG TACATACATT TGGAGAGATT GGGCTTCAGA AGCAACATGA TTCCTTCAAT 
CTCACCCTCT CTGACCTCTC TGATGAGTGG GTGGTGGGAG GAAACAAGGT GCAGGGGGTG 
AGGGTGCAAG TAACCATCTT ACCAGTAGAC AGTGAAGCTC CAGTAGTAAC ATTAAAAGAA 
AAGTTCTCTG TTTTGGAAGG TGAGAAAAAT GTCATCACCT ATGAAAACCT GGACGCAGAT 
GACAAGGACA CCCCTAAAGA TGACATCTTG TGCACTATTA TTGTCCAGCC TACTTCTGGC 
TATGTAGAGA ACATTTCACC TGCCCCTGGC TCTGAAAAGT CAAGGTCTGG GACAGCAGTC 
AGTGCATTTA CAATAAGAGA TATCCGACTG GGTCACATTT ACTATGTTCA AAGCATCCAT 
CAGGGTGTAG AACCTCAGGA AGATAGATTC ACTTTTCACT GCTCTGATGG AATAAGCTCT 
TCACAGCGCC TTTTTTTCCC CATAGTGATC ATCCCAACCA ATGATGAGAA ACCAGAACTT 
TTCACACGGG AATTTGTAGT CATGGAAGGC ATGAGCCTGG TGATTGACAC CCCAATCCTT 
AATGCTGCAG ATGGAGACCA ACCACCGGAT GAACTTACCT TTATAATATC CAAGCCACCA 
CTACACGGTA GGGTGGTCCA ACAGCAAGCA ACAGGCACTG TCCCCATTCT GAATTTTACT 
TTAGAAGAAA TCAAAGAGAG CTCAAGCATT GTATATGAGC ATGACGATTC AGAAACCAAG 
GAGGACAGCT TTGAGATTAT CTTAACTGAT GGAGAACACA ATGTAATAAA GAAAGTCCTT 
ATTATGGTTA TTCCCGTGGA TGATGAGACT CCAAGAATGG CCATCAATGA TGGACTTGAA 
ATTGATATTG GAGAGTCAAA GGTAATCACA AACAGGGTTT TAAAGGCTAC AGACTTAGAT 
TCTGATGACA AGTCATTGGC TTATGTTATT CGGTTTGGCC CCAGCCAAGG TTTTCTGCAG 
CGCTTGGACA AACATGGGTC AGTGGTTGGT AATATTACCT TGGGAATGAA TTTTACTCAA 
GAGGACATTG ACAGAGGTTT AATTCGGTAC ACCCATGCTG GTCAGGAAGG CATACGGGAC 
CTTTTTAAAT TTGACATAAC AGATGGGATT AACCCATTCA TAGACAGATA CTTTTATATA 
AGCATTGGCA GCATTGATAT TGTTTTCCCA GATGTCATAA ACAAAGGAGT TACTCTCAAA 
GAAGGCGGAA AGGTGACACT TACTACAGAC CTTCTAAGTA CAAGTGACAT TAACAGTCCA 
GATGAAAATC TGCGCTTCAG TATTACAAGG GCCCCAAGCC GTGGGCACTT AGAGAGCACT 
GATGCCCCTG GTGTTCCCAT TACATCTTTT ACTCAGCTCC AACTAGCTGG GAATAAGGTC 
TATTACATCC ACACTTCAGA GGATGAGATG AAGATAGACA GTTTTGAGTT TGAAGTGACA 
GATGGTTATA ACCCAGTTTT CCGTACTTTT AGGGTCTCTA TTACTGATGT AGATAATAAA 
AAGCCAGTCT TGATGATAAA TGGACTAGTG GTCAATGAAG GAGAAAATAA GCTTATAACT 
CCCTTTGAGC TAAGTGTGGA GGACAGGGAC ACCCCAGATA ATTTGCTGCG ATTTACAATC 
ACTCAAGTGC CTGTTCATGG TCAGATTGTG TTCAACAACA GCCAGACAGT TACTACTTTC 
ACCAAGCAAG ACCTGAATGA GAATCTCATT AGCTACAAGC ATGATGGCAG TGAGTCTGTA 
GAAGATAGCT TTTCCTTCAC AGTCACTGAT GGGACCCATG CAGAGTTTTT TGTACTGCCA 
GACACAACCT TTGAAAGCAA GAAACCCCAA ATGATGAAGA TCAGAATAAA TTCTTTGGAC 
AATGGTGTAC CCCAACTTAT TGTCAACAAG GGGGCCCCTA CTCTTAGAAG CCTACATCCT 
GGCCAACTTG GGTTCACGAT CACCAGCAAA TCCCTAAAGG CAGAAGATCG GGACAGCCCT 
CACAAGATGC TCAAATACAG AATCCTAGAT GGACCTAAAC ATGGGTTTTT AGTCAACTTG 
GACCATGGCA ATGACAGCAT CAGAACCTTC ACACAGGGCG ACATTGATGA CATGAAAATC 
TGCTATGTTC TGAAGGATGG TGACAATGCT ACAGGAGATA TTTTCCATTT CTCAGTAGAA 
GATATAGGGG GCAATAAACT AAGGAGTCAG CAATTTCGCC TGAACTGGGC TTGGATTTCA 
ATGGAAAAAA GCCATTATAC TGTAGACGAA GATTCAAAGT TTCTGGAAGT GACACTTAAA 
CGAAGAGGAT ACCTTGGAGA GACATCTTTT ATCAGCATCA CTACAAAAGA TGGCACAGCA 
ACCAAGGATA AAGACTTTAA AGGAAAAGCC CAGAAGCAGG TTCAGTTTAA CCCCGGGCAG 
ACTACAGCCA CATGGAAAGT GCGAATTCTT GCAGATAATG AATATGAGAA TTCTGAGACA 
TTTCATGTTA TCTTATCAGA ACCTGTTATG GCTGCCCTTG AGTTTCCAAT AGAAGCAACA 
ATTGAAATTG TAGATCCAGG TGATGAGTCC ACTGTTTACA TACCGCAAGC AGAATACAAG 
ATAGAGGAAG ATATTGGAGA ACTGATAATC CCTATTAAAA GATCAGGCGA CATAAGTCAA 
GAACTTATGG TAGTTTGTTC AACGCATCAA GGTACAGCTA CAGGCACTGT TCCCTCCTCT 
GTATTGTCCT ACTCTGACTA CATCACACGT GCAGATGGAC ACACAAGTAT TATTCGCTTT 
GACAAAGATG AGACTGAGAA AAACTGCCGT GTTATTATCA TTGATGACTC CCTGTATGAA 
GGAGACGAGA CATTTAATGT CAGCCTTAGT ATGCCCATGG GAGGACAGCT GGGAGAATTT 
CCAAGTACTA AAGTGGTTAT CCTTGCTGAT ACAGATGATG AGCCAGCCTT CTACTTTGCA 
GATACAGATT ATCATGTTGA TGAGAGTGCT GGGTATGTTG AAGTTCGTGT TTGGAGAACA 
GGGACAGATC TGACCAAAGC AGCCACGGTC ACTGTGCGTT CCAGGAAAAG CGAGCCTCTG 
TCTGCAGAAG CTGGAACAGA TTATGTGGGT ATCAGCAGAA ACCTTGATTT TGCCCCTGGT 
GTAACCATGC AGAGCTTCCG AGTAGTAATT CTTGATGATC TGGGGCAGCC TTCTCTGGAG 
GGACTTGAGA AATTTGAATT GGTTCTTCAA ATGCCCATGG GAGCAGTATT GGGGGAGCCC 
AGCAAAACAA CTGTGACCAT CAATGATACC ATCTCTGACT TACCTAAGGT TCAGTTTAAG 
GAGTCAGCTT ATTATGCCAA CGAGAAAGAT GGGCAAGTAT CTGCCGTTAT ATACCGCAGT 
GGTGATGTCA GCCATAAATC CTCAGTGCGT TGCTTCACCC GCCAAGGATC GGCGCAAGTC 
ACAATGGATT ATAACGAAAG GCCAAACACT GATGATTCTG CTGTCGTCTT CTTGCCTGGT 
GAGACTGAGA AGGCATGTGT GGTAACCCTG GAAGATGATA AAGTATATGA AGAAGAAGAA 
GAGTTCCGAT TGCTACTTGG AACACCCAAA AGTGACTCAC CTTTTGGAGC CTCAGTTGGA 
GAGTCTAAAG AAGCTCTCAT AAAGATTAAA GATGCGGAGG ACAAACCAAT TATCAGGTTT 
TCAGAAATCA AGTATAGTAT CCAGGAGCCC CAGGAGTCCG GTGAAATAGC TGTTGTGAAA 
ATCCCTGTGC TGCGAATTGG AGACAACTCC AAGGTGTCCA TTGTTAGAAT TCACACCAAA 
GATGGCTCTG CTACATCAGG AGAAGATTAT AACCCATTGT CTGAAGATAT TGAGTTTAAA 
GAAGGAGAAA CCGAACACTT TGTTGAAGTG GAGATATTAT ATGATGGAGT AAGAGAAATG 
CGTGAAGCAT TTACTATACA CCTGAAGCCT GATGAGAACA TGGTAGCTGA AATCCAGATG 
AATAAAGCCA TAGTGTATAT TGAAGAGATG GACAGTGTTG CTGATGTGAC TTTTCCAGCT 
GTACCACAGG TTGTCTCACT TTTGCTGTAT GATGACACCT TCAAAATCAA GGAAAATCCC 
AGTCCCCCCA CTGGTTACCC AGTTGTATGT GTAACGGCGT GTAATCCAAA ATACTATGAC 
TATGACAAAA CTGGATCAAT CTGTGCTGCT GAAAACATTA ATGATACTCT GACCCAGTAC 
CGCTGGCTCG TCAGCGCTCC AAGTGGAAGT GATGGTGTCA CCAGCCCCAT GCGTGAAGTT 
GACTCCAATA CGTTCTTCAC TGACACTAAG TCGATCACAT TGGACTCTGT CTACTTCCAT 
GCTGGATCAA GAATCCAATG TGCAGCCAGG GCAGTCAATG CAAATGGAGA CTCTGGTCTG 
GAATTGCTAA GCCCTATATT TAGTGTTAGC AGAGAGGAAG GTCTTTGTCA GCCTCGTATT 
CCTGGAACAG TGGGTGCTGA ACCATTCTCA GCAAAAATCC GTTACACAGG CCCTGAAGAT 
CCTGACTACC CTAATCTGAT AAAACTGACA GTAACAATGC CACATATGGA CGGTATGCTT 
CCAGTCATCT CAACCAGACA CATGTCCAAC TTTGAATTGA CGCTTAGTCC AGATGGCACA 
AGAGTTGGAA ACCACAAGTG CTCCAACCTT CTAGATTACA ATGAGATTCC AACCAAGCAC 
AGCTTTATTA CAGACAATAC CAAGAGCCCT GAAGTGATCG GAGAAACATC CCCCTACCAG 
TACAGCAATG CTCTTCGATC TGCTAGAACT CTACGCTTCT ATCGCAACCT CAATTTGGAG 
GCCTGTCTGT GGGAATTTAA CAGTTACTAT GACATGTCTG AACTTCTGAC AGATTGTGGC 
GGAACTATAG GGACAGATGG ACAAGTGCTG AATCTGGTCC AGTCCTATGT AACTCTACGG 
GTTCCCCTCT ATGTATCCTA TGTGTTTCAC TCGGCAGCAG CAGTTGGTGG GTGGCAGCAC 
TTTGACCTGC AGTCAGAGTT AAGGTTGACA TTTGTGTATG ATACTGCCAT CTTATGGAAC 
GAAGGAATAG GCAGCCCACC AGAATCTGAG CTACAGGGAT CCCTTTATCC AACGAGCATG 
CGCATTAATG ATGAAGGACG CTTGGTAGTC AATTTTAAGA CAGAGATTCG CTTTCGGGGA 
CAGTTTGTCA TTTCTCATCC AGGTACATCT CTTTCATCCA TGGTGATGTC TGCCGACCAT 
CCTGAACTCA CCTTTACATT GAGTCTGTTG CGCAGTGAGC CAAGCTTCAA TCAACCCGTT 
CAGCAATGGA GCTTCATCTC CGACTTTGCT GTACGTGATT ATTCTGGCAC ATATACAGTG 
AAGCTTATCC CATGTAGCAC CACCCCAAAC CAAGAATATT CTTTACCTGT TATCTGCAAT 
CCACGTGAAC CTATATCCTT CAATATCGAC ATTAGGTTCC AACAGGTTAG TGACCCAGTG 
GCAGCAGAAT TCAGTCTTAA CACCCAAATG TTGTTGCTGT CTAAAAAAGA ATTGTGGTTA 
TCAGATGGCT CAATGGGATT TGGCCAGGGA AGTGATGTTG CATTTACTGA AGGCTCTACA 
ATCTATGGGC GAATAATGGT GGATCCAGTT CAGAACCTTG GGGACTCTTT TATCTGCAAC 
ATTGAGAAAG TGTTTTTATG CACTGGTTCT GATGGTTATG TCCCCAAATA CAGCCCAGAA 
AACAAGGAAT ATGGATGCTT TGCGGACTCG CCAACATTAT TGTACAGATT TAAAATACTG 
GACAAAGCAC AGCCAGAAAC ACAGGCCAGG TTATTTGGAG ATATTCCATT CGAAGCCAGA 
TTAGCTATTG ATAACCCCAG TGCTCTGGCG CTGGTGAAGC AACCAGGTTC TGATGGTTTC 
AGCCTCTCAT CTGCGCCACT GTTTCAGGTG GCTTCTGGAC GGGAGTGGTT TATTCATACT 
ATTTACACAG TCCGCTCTAA GGAGAATGCA AACCGAGGGA TTGGAAAGCG TAGTGTGGAT 
TACCATCATG CCTTGAGTAG CGCTACTGAC CCCAGGGGGG AACTCACACT AAAGAGAAGC 
CGGAGAGCAT ATCCAGAAGA TTCCGCATTT GCACAGGACA TTGGTGCTGA TAAGAACAGA 
GGGACCAACA TTCAGCACAT TGCTTTGGAG CAGAGCAGCA AGGCCTCAGG TTACAATAAG 
GACATGTTGC CTGGTGGTGG CCAATATCAG AAGCCAGTCT TGGAAGTAAC AGGCCACAAC 
TCAGAGGGAC CTTTGGTGCC TGTTCTTGGA GGGGTGGGTG CTTTATTGCT CTTAATCTGC 
ACTGTGGTTA TCATTGGTCT GCTCGTGAAA CACCGGAGAC GGCAGCACTC TGAGAAGAAG 
TCTCCAACTA ATAGTTATCA TAACAGCCAT GAAACTATGA GTACAAGGCA CAGCAGCAGT 
GACAGCTCGG AAGTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

frem3 ( XM_002940081.5 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_002940127.3 Xenopus tropicalis FRAS1 related extracellular matrix 3 (frem3), mRNA.

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CloneID	OXa49882
Clone ID Related Accession (Same CDS sequence)	XM_002940081.5
Accession Version	XM_002940081.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 9378bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1576512000000
Organism	Xenopus tropicalis(tropical clawed frog)
Product	FRAS1-related extracellular matrix protein 3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_002940081.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGGACCC ACAAACACCT ACCTACAAGC TTAGGCTGCT TCACCCTGAT GTTAGCCGCT 
CTCAGCTGGG CAGCTGCAGA TGGCGACTCC CGTTTGTCCG GCCCAGATAA CATTATAGTA 
ACCAACCGAA GGATCCGGGT GCCCTTGGGC AGATCTGTGT TCCTGGACCC AGTGCAAGAC 
CTAGCGATCC AGGTGCAGCC CGGAGACAGC TGTATCCTCA GAGTGTTGGC CAATGACCCT 
CTGTCTCAGC GCCCCGGCTT CTTAACCCCT AAGCGATTTC CGTGCGATTT CGGTGCTGGG 
GAGGTAGAAT ACACTCACCT GGGCGCCCGT AGCCCTGCCT CGGACAGAGT GAGGATGCAG 
CTGAAGTACG ACTCGCCCAC ACACACCTGG ATTGTGCCCT TTGTGATCGA AGTGGAGCTG 
ATCTTCCAGC AGCTGGAGAT AGTGACCCGC AACTTGCCGC TGTTGGTGGA TTCGCCGCGG 
GAAATCAGCC CCCCAGTTGA TAAGAAGGTG CTGGGCTTCT CCTACGATCC AACCAAGGAG 
TCGTGTAAGA TCAGCCCGCT GATTGGCACA GGGGGTCTGC CCAGGTACGG CCAACTGCTC 
AATGGCACTG CCGCCCCTCA GCTGGTCACT GACTGTCACA CCTTCATCAA CTCGGGGCTC 
CGCTACCAGC ACACCGCGCC CCAAAACTCG CCCAACAGAG ACTATGTGCC CATGCTGGCC 
CAGCTCAATG ATGGGCACGG AGTAGCCAGG CAGGAACACT TCCAGCTCAC CGTTATGATC 
AAGGGGGGAG CGGAGAACAC AGCCCCCCGG CCCAGCTTTG TGTCTATGAT GATGATGGAA 
GTGGGTCAGT TCGTGCTGAC AGCCCTCACC CCGGAGATGT TGGCGGCTGA GGATGCGGAG 
TCGGAACCGG GCGAGCTTAT CTTTAACGTG ACTAGCTCTC TGGATCCGGA GACATCGGGC 
TACCTGATCA GCACCGACGA TCGCAACCTG CCCATTACTT CTTTCTACCA GCGGGACCTG 
ATGGAGCTGA GGATCGCGTA CCAGCCCCCG GCTAGTGAGT CCTGGCAGGA GCGGATCATT 
CAGCTGGAGA TGCATGTCAT TGACACCGAG GGGGCATCGT CCGATTACTT TGCGTTTATG 
ATCGTTGTAA AGCCCATGAA CACACTGGCC CCACTGGCGA CTCGCAACGC TGGCTTGCGG 
CTCCTAGAGG GTCAGTCTCG CCCTTTGTCC GGCCCAGGCT CCGGCATGAA CAACCTCGTT 
ATAAGCGATG AGGACAACCT GGACCAAGTC AGGATCAGTG TGATCGATGG TCTCCGGCAC 
GGCCAGTTAA TCCTAAGCCC CAGTGACAGC GGGACCAATA GTGGCCACAA GGACTTCTCC 
TTTAGCCCAC AGGAACTTCA CTCGGGGCTG GTGATTTATC AGCACGATGG CAGCGACACC 
TACAGCGACA ATCTGATCCT AAGGCTGGAC GATGGCAAGC ATCGGGTGGA GCTGCTGTAC 
CCCATCTCAG TGGCCCCTAC TGATGATGAG CCCCCAGTAC TGAATGCCAA CACCGGGCTG 
AACCTACGGG AGAAGGGCCT GGCTCTCATC TCCCCTTTCA TGCTCAGTGC CACAGACATC 
GACTCGGAAG ATTCCACCAT ACACTTTGTG CTGCAGGAAC CCCTCGGCCG ACTCGGGGAG 
ATGCTGCTGA GGCAGCCGGA TGAGCCGGAG GGGGAGGAGG GCTGGAAATA TCTGCCCCAG 
GAAGGGATTT ACGAGAAGGG GGTGATCGAG TGGTACCAGA AGGATATTCT GGATGGGAAA 
CTGCACTACA GGCACCTGGG TTCGCATAGT ACAACTACAG TTCTGGACCG AGTGCTGTTC 
GTGGTGCTGG ATGACCATGA CCCCCCAAAC CAGTCCAATG AGCAGGTATT TATGGTGCGC 
ATAGAGCCCC TTGATGACAT CCCCCCAGAA CTGTACCCCG GTACTACCCT GCACATGACT 
GTCAAGGAAT ACGAGGTGAC CCCCTTCAGG AAGAAGTACC TGCGCTACAC TGATCTGGAC 
ACAGACGACC GAGAGCTTAA ATATACCTTG CACAGCCACC CTAGGGATAC CGACCCCAAC 
CATCCTGCCC TGACTGGCCA GATTGTGCTG AGTGACAGCC TGGGGGTCGA GGTGAACCAA 
TTTACACAAG CCCAGATCAA CCATTTAAAG ATCTCATACA AGCCCCCAGA GAAAGAACTG 
GGCATAGTTC CCAGGGAGCT ACAGTTCAAC TACACAGTGG AGGATGCTGA GGGAAATTCG 
GTGCAAGGGA CATTCACTAT AGTGTTACAG CCAGTGGACA ATAAACCTCC GGAGATCACA 
AATACTGGTT TCACTGTCCC AGAGAGGGGT TCCTTCACTC TCACCAAAAA TGAACTGGAT 
GCCACAGATC AAGACACAGA CACAAGCAAC ATCATTTTCA GCTTGTCTGA AGCCCCTCAG 
CATGGGGAGT TACAGTACCA GGGTGTACCA ATGTCCTTGG GAGAGTCCTT TGCTTTAAGT 
GACATTTCAC TGGGTTGGGT ATCCTATGTT CATAGTGGAG AGGAGACCAC CAGCGACACA 
TGTTCCCTGG TAGTTAGTGA TGGTGTTCAC GAAGTTCCCA TTACAGTCCG AATTAAAGTA 
AAGCCAGTGG ATGACGAGGC ACCCATTTTG ATGCTTGCTG CAGGTCTTTT GGGGTCATCC 
ATTGAAGTCT TGGAGAGAGG AGAAACTGAG ATTACCAGCA GTGTTGTTCA GGGCACAGAT 
CCAGACACTG ATGATTTGAT GCTCACATTC ATTGTGGAGG AGCCTCCCAG AAAAGGGGAT 
ATATTGGTCA ATGGAGTTAC CGCTGAGAGG TTCTCTCAGC AAGACCTTCT CAATGGTGCT 
GTTACATATG TACATACATT TGGAGAGATT GGGCTTCAGA AGCAACATGA TTCCTTCAAT 
CTCACCCTCT CTGACCTCTC TGATGAGTGG GTGGTGGGAG GAAACAAGGT GCAGGGGGTG 
AGGGTGCAAG TAACCATCTT ACCAGTAGAC AGTGAAGCTC CAGTAGTAAC ATTAAAAGAA 
AAGTTCTCTG TTTTGGAAGG TGAGAAAAAT GTCATCACCT ATGAAAACCT GGACGCAGAT 
GACAAGGACA CCCCTAAAGA TGACATCTTG TGCACTATTA TTGTCCAGCC TACTTCTGGC 
TATGTAGAGA ACATTTCACC TGCCCCTGGC TCTGAAAAGT CAAGGTCTGG GACAGCAGTC 
AGTGCATTTA CAATAAGAGA TATCCGACTG GGTCACATTT ACTATGTTCA AAGCATCCAT 
CAGGGTGTAG AACCTCAGGA AGATAGATTC ACTTTTCACT GCTCTGATGG AATAAGCTCT 
TCACAGCGCC TTTTTTTCCC CATAGTGATC ATCCCAACCA ATGATGAGAA ACCAGAACTT 
TTCACACGGG AATTTGTAGT CATGGAAGGC ATGAGCCTGG TGATTGACAC CCCAATCCTT 
AATGCTGCAG ATGGAGACCA ACCACCGGAT GAACTTACCT TTATAATATC CAAGCCACCA 
CTACACGGTA GGGTGGTCCA ACAGCAAGCA ACAGGCACTG TCCCCATTCT GAATTTTACT 
TTAGAAGAAA TCAAAGAGAG CTCAAGCATT GTATATGAGC ATGACGATTC AGAAACCAAG 
GAGGACAGCT TTGAGATTAT CTTAACTGAT GGAGAACACA ATGTAATAAA GAAAGTCCTT 
ATTATGGTTA TTCCCGTGGA TGATGAGACT CCAAGAATGG CCATCAATGA TGGACTTGAA 
ATTGATATTG GAGAGTCAAA GGTAATCACA AACAGGGTTT TAAAGGCTAC AGACTTAGAT 
TCTGATGACA AGTCATTGGC TTATGTTATT CGGTTTGGCC CCAGCCAAGG TTTTCTGCAG 
CGCTTGGACA AACATGGGTC AGTGGTTGGT AATATTACCT TGGGAATGAA TTTTACTCAA 
GAGGACATTG ACAGAGGTTT AATTCGGTAC ACCCATGCTG GTCAGGAAGG CATACGGGAC 
CTTTTTAAAT TTGACATAAC AGATGGGATT AACCCATTCA TAGACAGATA CTTTTATATA 
AGCATTGGCA GCATTGATAT TGTTTTCCCA GATGTCATAA ACAAAGGAGT TACTCTCAAA 
GAAGGCGGAA AGGTGACACT TACTACAGAC CTTCTAAGTA CAAGTGACAT TAACAGTCCA 
GATGAAAATC TGCGCTTCAG TATTACAAGG GCCCCAAGCC GTGGGCACTT AGAGAGCACT 
GATGCCCCTG GTGTTCCCAT TACATCTTTT ACTCAGCTCC AACTAGCTGG GAATAAGGTC 
TATTACATCC ACACTTCAGA GGATGAGATG AAGATAGACA GTTTTGAGTT TGAAGTGACA 
GATGGTTATA ACCCAGTTTT TCGTACTTTT AGGGTCTCTA TTACTGATGT AGATAATAAA 
AAGCCAGTCT TGATGATAAA TGGACTAGTG GTCAATGAAG GAGAAAATAA GCTTATAACT 
CCCTTTGAGC TAAGTGTGGA GGACAGGGAC ACCCCAGATA ATTTGCTGCG ATTTACAATC 
ACTCAAGTGC CTGTTCATGG TCAGATTGTG TTCAACAACA GCCAGACAGT TACTACTTTC 
ACCAAGCAAG ACCTGAATGA GAATCTCATT AGCTACAAGC ATGATGGCAG TGAATCTGTA 
GAAGATAGCT TTTCCTTCAC AGTCACTGAT GGGACCCATG CAGAGTTTTT TGTACTGCCA 
GACACAACCT TTGAAAGCAA GAAACCCCAA ATGATGAAGA TCAGAATAAA TTCTTTGGAC 
AATGGTGTAC CCCAACTTAT TGTCAACAAG GGGGCCCCTA CTCTTAGAAG CCTACATCCA 
GGCCAACTTG GGTTCACGAT CACCAGCAAA TCCCTAAAGG CAGAAGATCG GGACAGCCCT 
CACAAGATGC TCAAATACAG AATCCTAGAT GGACCTAAAC ATGGGTTTTT AGTCAACTTG 
GACCATGGCA ATGACAGCAT CAGAACCTTC ACACAGGGCG ACATTGATGA CATGAAAATC 
TGCTATGTTC TGAAGGATGG TGACAATGCT ACAGGGGATA TTTTCCATTT CTCAGTAGAA 
GATATAGGGG GCAATAAACT AAGGAGTCAG CAATTTCGCC TGAACTGGGC TTGGATTTCA 
ATGGAAAAAA GCCATTATAC TGTAGACGAA GATTCAAAGT TTCTGGAAGT GACACTTAAA 
CGAAGAGGAT ACCTTGGAGA GACATCTTTT ATCAGCATCA CTACAAAAGA TGGCACAGCA 
ACCAAGGATA AAGACTTTAA AGGAAAAGCC CAGAAGCAGG TTCAGTTCAA CCCCGGGCAG 
ACTACAGCCA CATGGAAAGT GCGAATTCTT GCAGATAATG AATATGAGAA TTCTGAGACA 
TTTCATGTTA TCTTATCAGA ACCTGTTATG GCTGCCCTTG AGTTTCCAAT AGAAGCAACA 
ATTGAAATTG TAGATCCAGG TGATGAGTCC ACTGTTTACA TACCGCAAGC AGAATACAAG 
ATAGAGGAAG ATATTGGAGA ACTGATAATC CCTATTAAAA GATCAGGCGA CATAAGTCAA 
GAACTTATGG TAGTTTGTTC AACGCATCAA GGTACAGCTA CAGGCACTGT TCCCTCCTCT 
GTATTGTCCT ACTCTGACTA CATCACACGT GCAGATGGAC ACACAAGTAT TATTCGCTTT 
GACAAAGATG AGACTGAGAA AAACTGCCGT GTTATTATCA TTGATGACTC CCTGTATGAA 
GGAGACGAGA CATTTAATGT CAGCCTTAGT ATGCCCATGG GAGGACAGCT GGGAGAATTT 
CCAAGTACTA AAGTGGTTAT CCTTGCTGAT ACAGATGATG AGCCAGCCTT CTACTTTGCA 
GATACAGATT ATCATGTTGA TGAGAGTGCT GGGTATGTTG AAGTTCGTGT TTGGAGAACA 
GGGACAGATC TGACCAAAGC AGCCACGGTC ACTGTGCGTT CCAGGAAAAG CGAGCCTCTG 
TCTGCAGAAG CTGGAACAGA TTATGTGGGT ATCAGCAGAA ACCTTGATTT TGCCCCTGGT 
GTAACCATGC AGAGCTTCCG AGTAGTAATT CTTGATGATC TGGGGCAGCC TTCTCTGGAG 
GGACTTGAGA AATTTGAATT GGTTCTTCAA ATGCCCATGG GAGCAGTATT GGGGGAGCCC 
AGCAAAACAA CTGTGACCAT CAATGATACC ATCTCTGACT TACCTAAGGT TCAGTTTAAG 
GAGTCAGCTT ATTATGCCAA CGAGAAAGAT GGGCAAGTAT CTGCCGTTAT ATACCGCAGT 
GGTGATGTCA GCCATAAATC CTCAGTGCGT TGCTTCACCC GCCAAGGATC GGCGCAAGTC 
ACAATGGATT ATAACGAAAG GCCAAACACT GATGATTCTG CTGTCGTCTT CTTGCCTGGT 
GAGACTGAGA AGGCATGTGT GGTAACCCTG GAAGATGATA AAGTATATGA AGAAGAAGAA 
GAGTTCCGAT TGCTACTTGG AACACCCAAA AGTGACTCAC CTTTTGGAGC CTCAGTTGGA 
GAGTCTAAAG AAGCTCTCAT AAAGATTAAA GATGCGGAGG ACAAACCAAT TATCAGGTTT 
TCAGAAATCA AGTATAGTAT CCAGGAGCCC CAGGAGTCCG GTGAAATAGC TGTTGTGAAA 
ATCCCTGTGC TGCGAATTGG AGACAACTCC AAGGTGTCCA TTGTTAGAAT TCACACCAAA 
GATGGCTCTG CTACATCAGG AGAAGATTAT AACCCATTGT CTGAAGATAT TGAGTTTAAA 
GAAGGAGAAA CCGAACACTT TGTTGAAGTG GAGATATTAT ATGATGGAGT AAGAGAAATG 
CGTGAAGCAT TTACTATACA CCTGAAGCCT GATGAGAACA TGGTAGCTGA AATCCAGATG 
AATAAAGCCA TAGTGTATAT TGAAGAGATG GACAGTGTTG CTGATGTGAC TTTTCCAGCT 
GTACCACAGG TTGTCTCACT TTTGCTGTAT GATGACACCT TCAAAATCAA GGAAAATCCC 
AGTCCCCCCA CTGGTTACCC AGTTGTATGT GTAACGGCGT GTAATCCAAA ATACTATGAC 
TATGACAAAA CTGGATCAAT CTGTGCTGCT GAAAACATTA ATGATACTCT GACCCAGTAC 
CGCTGGCTCG TCAGCGCTCC AAGTGGAAGT GATGGTGTCA CCAGCCCCAT GCGTGAAGTT 
GACTCCAATA CGTTCTTCAC TGACACTAAG TCGATCACAT TGGACTCTAT CTACTTCCAT 
GCTGGATCAA GAATCCAATG TGCAGCCAGG GCAGTCAATG CAAATGGAGA CTCTGGTCTG 
GAATTGCTAA GCCCTATATT TAGTGTTAGC AGAGAGGAAG GTCTTTGTCA GCCTCGTATT 
CCTGGAACAG TGGGTGCTGA ACCATTCTCA GCAAAAATCC GTTACACAGG CCCTGAAGAT 
CCTGACTACC CTAATCTGAT AAAACTGACA GTAACAATGC CACATATGGA CGGTATGCTT 
CCAGTCATCT CAACCAGACA CATGTCCAAC TTTGAATTGA CGCTTAGTCC AGATGGCACA 
AGAGTTGGAA ACCACAAGTG CTCCAACCTT CTAGATTACA ATGAGATTCC AACCAAGCAC 
AGCTTTATTA CAGACAATAC CAAGAGCCCT GAAGTGATCG GAGAAACATC CCCCTACCAG 
TACAGCAATG CTCTTCGATC TGCTAGAACT CTACGCTTCT ATCGCAACCT CAATTTGGAG 
GCCTGTCTGT GGGAATTTAA CAGTTACTAT GACATGTCTG AACTTCTGAC AGATTGTGGC 
GGAACTATAG GGACAGATGG ACAAGTGCTG AATCTGGTCC AGTCCTATGT AACTCTACGG 
GTTCCCCTCT ATGTATCCTA TGTGTTTCAC TCGGCAGCAG CAGTTGGTGG GTGGCAGCAC 
TTTGACCTGC AGTCAGAGTT AAGGTTGACA TTTGTGTATG ATACTGCCAT CTTATGGAAC 
GAAGGAATAG GCAGCCCACC AGAATCTGAG CTACAGGGAT CCCTTTATCC AACGAGCATG 
CGCATTAATG ATGAAGGACG CTTGGTAGTC AATTTTAAGA CAGAGATTCG CTTTCGGGGA 
CAGTTTGTCA TTTCTCATCC AGGTACATCT CTTTCATCCA TGGTGATGTC TGCCGACCAT 
CCTGAACTCA CCTTTACATT GAGTCTGTTG CGCAGTGAGC CAAGCTTCAA TCAACCCGTT 
CAGCAATGGA GCTTCATCTC CGACTTTGCT GTACGTGATT ATTCTGGCAC ATATACAGTG 
AAGCTTATCC CATGTAGCAC CACCCCAAAC CAAGAATATT CTTTACCTGT TATCTGCAAT 
CCACGTGAAC CTATATCCTT CAATATCGAC ATTAGGTTCC AACAGGTTAG TGACCCAGTG 
GCAGCAGAAT TCAGTCTTAA CACCCAAATG TTGTTGCTGT CTAAAAAAGA ATTGTGGTTA 
TCAGATGGCT CAATGGGATT TGGCCAGGGA AGTGATGTTG CATTTACTGA AGGCTCTACA 
ATCTATGGGC GAATAATGGT GGATCCAGTT CAGAACCTTG GGGACTCTTT TATCTGCAAC 
ATTGAGAAAG TGTTTTTATG CACTGGTTCT GATGGTTATG TCCCCAAATA CAGCCCAGAA 
AACAAGGAAT ATGGATGCTT TGCGGACTCG CCAACATTAT TGTACAGATT TAAAATACTG 
GACAAAGCAC AGCCAGAAAC ACAGGCCAGG TTATTTGGAG ATATTCCATT CGAAGCCAGA 
TTAGCTATTG ATAACCCCAG TGCTCTGGCG CTGGTGAAGC AACCAGGTTC TGATGGTTTC 
AGCCTCTCAT CTGCGCCACT GTTTCAGGTG GCTTCTGGAC GGGAGTGGTT TATTCATACT 
ATTTACACAG TCCGCTCTAA GGAAAATGCA AACCGAGGGA TTGGAAAGCG TAGTGTGGAT 
TACCATCATG CCTTGAGTAG CGCTACTGAC CCCAGGGGGG AACTCACACT AAAGAGAAGC 
CGGAGAGCAT ATCCAGAAGA TTCCGCATTT GCACAGGACA TTGGTGCTGA TAAGAACAGA 
GGGACCAACA TTCAGCACAT TGCTTTGGAG CAGAGCAGCA AGGCCTCAGG TTACAATAAG 
GACATGTTGC CTGGTGGTGG CCAATATCAG AAGCCAGTCT TGGAAGTAAC AGGCCACAAC 
TCAGAGGGAC CTTTGGTGCC TGTTCTTGGA GGGGTGGGTG CTTTATTGCT CTTAATCTGC 
ACTGTGGTTA TCATTGGTCT GCTCGTGAAA CACCGGAGAC GGCAGCACTC TGAGAAGAAG 
TCTCCAACTA ATAGTTATCA TAACAGCCAT GAAACTATGA GTACAAGGCA CAGCAGCAGT 
GACAGCTCGG AAGTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_002940127.3
CDS	149..9526
Translation	MGTHKHLPTSLGCFTLMLAALSWAAADGDSRLSGPDNIIVTNRRIRVPLGRSVFLDPVQDLAIQVQPGDSCILRVLANDPLSQRPGFLTPKRFPCDFGAGEVEYTHLGARSPASDRVRMQLKYDSPTHTWIVPFVIEVELIFQQLEIVTRNLPLLVDSPREISPPVDKKVLGFSYDPTKESCKISPLIGTGGLPRYGQLLNGTAAPQLVTDCHTFINSGLRYQHTAPQNSPNRDYVPMLAQLNDGHGVARQEHFQLTVMIKGGAENTAPRPSFVSMMMMEVGQFVLTALTPEMLAAEDAESEPGELIFNVTSSLDPETSGYLISTDDRNLPITSFYQRDLMELRIAYQPPASESWQERIIQLEMHVIDTEGASSDYFAFMIVVKPMNTLAPLATRNAGLRLLEGQSRPLSGPGSGMNNLVISDEDNLDQVRISVIDGLRHGQLILSPSDSGTNSGHKDFSFSPQELHSGLVIYQHDGSDTYSDNLILRLDDGKHRVELLYPISVAPTDDEPPVLNANTGLNLREKGLALISPFMLSATDIDSEDSTIHFVLQEPLGRLGEMLLRQPDEPEGEEGWKYLPQEGIYEKGVIEWYQKDILDGKLHYRHLGSHSTTTVLDRVLFVVLDDHDPPNQSNEQVFMVRIEPLDDIPPELYPGTTLHMTVKEYEVTPFRKKYLRYTDLDTDDRELKYTLHSHPRDTDPNHPALTGQIVLSDSLGVEVNQFTQAQINHLKISYKPPEKELGIVPRELQFNYTVEDAEGNSVQGTFTIVLQPVDNKPPEITNTGFTVPERGSFTLTKNELDATDQDTDTSNIIFSLSEAPQHGELQYQGVPMSLGESFALSDISLGWVSYVHSGEETTSDTCSLVVSDGVHEVPITVRIKVKPVDDEAPILMLAAGLLGSSIEVLERGETEITSSVVQGTDPDTDDLMLTFIVEEPPRKGDILVNGVTAERFSQQDLLNGAVTYVHTFGEIGLQKQHDSFNLTLSDLSDEWVVGGNKVQGVRVQVTILPVDSEAPVVTLKEKFSVLEGEKNVITYENLDADDKDTPKDDILCTIIVQPTSGYVENISPAPGSEKSRSGTAVSAFTIRDIRLGHIYYVQSIHQGVEPQEDRFTFHCSDGISSSQRLFFPIVIIPTNDEKPELFTREFVVMEGMSLVIDTPILNAADGDQPPDELTFIISKPPLHGRVVQQQATGTVPILNFTLEEIKESSSIVYEHDDSETKEDSFEIILTDGEHNVIKKVLIMVIPVDDETPRMAINDGLEIDIGESKVITNRVLKATDLDSDDKSLAYVIRFGPSQGFLQRLDKHGSVVGNITLGMNFTQEDIDRGLIRYTHAGQEGIRDLFKFDITDGINPFIDRYFYISIGSIDIVFPDVINKGVTLKEGGKVTLTTDLLSTSDINSPDENLRFSITRAPSRGHLESTDAPGVPITSFTQLQLAGNKVYYIHTSEDEMKIDSFEFEVTDGYNPVFRTFRVSITDVDNKKPVLMINGLVVNEGENKLITPFELSVEDRDTPDNLLRFTITQVPVHGQIVFNNSQTVTTFTKQDLNENLISYKHDGSESVEDSFSFTVTDGTHAEFFVLPDTTFESKKPQMMKIRINSLDNGVPQLIVNKGAPTLRSLHPGQLGFTITSKSLKAEDRDSPHKMLKYRILDGPKHGFLVNLDHGNDSIRTFTQGDIDDMKICYVLKDGDNATGDIFHFSVEDIGGNKLRSQQFRLNWAWISMEKSHYTVDEDSKFLEVTLKRRGYLGETSFISITTKDGTATKDKDFKGKAQKQVQFNPGQTTATWKVRILADNEYENSETFHVILSEPVMAALEFPIEATIEIVDPGDESTVYIPQAEYKIEEDIGELIIPIKRSGDISQELMVVCSTHQGTATGTVPSSVLSYSDYITRADGHTSIIRFDKDETEKNCRVIIIDDSLYEGDETFNVSLSMPMGGQLGEFPSTKVVILADTDDEPAFYFADTDYHVDESAGYVEVRVWRTGTDLTKAATVTVRSRKSEPLSAEAGTDYVGISRNLDFAPGVTMQSFRVVILDDLGQPSLEGLEKFELVLQMPMGAVLGEPSKTTVTINDTISDLPKVQFKESAYYANEKDGQVSAVIYRSGDVSHKSSVRCFTRQGSAQVTMDYNERPNTDDSAVVFLPGETEKACVVTLEDDKVYEEEEEFRLLLGTPKSDSPFGASVGESKEALIKIKDAEDKPIIRFSEIKYSIQEPQESGEIAVVKIPVLRIGDNSKVSIVRIHTKDGSATSGEDYNPLSEDIEFKEGETEHFVEVEILYDGVREMREAFTIHLKPDENMVAEIQMNKAIVYIEEMDSVADVTFPAVPQVVSLLLYDDTFKIKENPSPPTGYPVVCVTACNPKYYDYDKTGSICAAENINDTLTQYRWLVSAPSGSDGVTSPMREVDSNTFFTDTKSITLDSIYFHAGSRIQCAARAVNANGDSGLELLSPIFSVSREEGLCQPRIPGTVGAEPFSAKIRYTGPEDPDYPNLIKLTVTMPHMDGMLPVISTRHMSNFELTLSPDGTRVGNHKCSNLLDYNEIPTKHSFITDNTKSPEVIGETSPYQYSNALRSARTLRFYRNLNLEACLWEFNSYYDMSELLTDCGGTIGTDGQVLNLVQSYVTLRVPLYVSYVFHSAAAVGGWQHFDLQSELRLTFVYDTAILWNEGIGSPPESELQGSLYPTSMRINDEGRLVVNFKTEIRFRGQFVISHPGTSLSSMVMSADHPELTFTLSLLRSEPSFNQPVQQWSFISDFAVRDYSGTYTVKLIPCSTTPNQEYSLPVICNPREPISFNIDIRFQQVSDPVAAEFSLNTQMLLLSKKELWLSDGSMGFGQGSDVAFTEGSTIYGRIMVDPVQNLGDSFICNIEKVFLCTGSDGYVPKYSPENKEYGCFADSPTLLYRFKILDKAQPETQARLFGDIPFEARLAIDNPSALALVKQPGSDGFSLSSAPLFQVASGREWFIHTIYTVRSKENANRGIGKRSVDYHHALSSATDPRGELTLKRSRRAYPEDSAFAQDIGADKNRGTNIQHIALEQSSKASGYNKDMLPGGGQYQKPVLEVTGHNSEGPLVPVLGGVGALLLLICTVVIIGLLVKHRRRQHSEKKSPTNSYHNSHETMSTRHSSSDSSEV

Target ORF information:

RefSeq Version	XM_002940081.5
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis FRAS1 related extracellular matrix 3 (frem3), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_002940081.5

ORF Insert Sequence:

ATGGGGACCC ACAAACACCT ACCTACAAGC TTAGGCTGCT TCACCCTGAT GTTAGCCGCT 
CTCAGCTGGG CAGCTGCAGA TGGCGACTCC CGTTTGTCCG GCCCAGATAA CATTATAGTA 
ACCAACCGAA GGATCCGGGT GCCCTTGGGC AGATCTGTGT TCCTGGACCC AGTGCAAGAC 
CTAGCGATCC AGGTGCAGCC CGGAGACAGC TGTATCCTCA GAGTGTTGGC CAATGACCCT 
CTGTCTCAGC GCCCCGGCTT CTTAACCCCT AAGCGATTTC CGTGCGATTT CGGTGCTGGG 
GAGGTAGAAT ACACTCACCT GGGCGCCCGT AGCCCTGCCT CGGACAGAGT GAGGATGCAG 
CTGAAGTACG ACTCGCCCAC ACACACCTGG ATTGTGCCCT TTGTGATCGA AGTGGAGCTG 
ATCTTCCAGC AGCTGGAGAT AGTGACCCGC AACTTGCCGC TGTTGGTGGA TTCGCCGCGG 
GAAATCAGCC CCCCAGTTGA TAAGAAGGTG CTGGGCTTCT CCTACGATCC AACCAAGGAG 
TCGTGTAAGA TCAGCCCGCT GATTGGCACA GGGGGTCTGC CCAGGTACGG CCAACTGCTC 
AATGGCACTG CCGCCCCTCA GCTGGTCACT GACTGTCACA CCTTCATCAA CTCGGGGCTC 
CGCTACCAGC ACACCGCGCC CCAAAACTCG CCCAACAGAG ACTATGTGCC CATGCTGGCC 
CAGCTCAATG ATGGGCACGG AGTAGCCAGG CAGGAACACT TCCAGCTCAC CGTTATGATC 
AAGGGGGGAG CGGAGAACAC AGCCCCCCGG CCCAGCTTTG TGTCTATGAT GATGATGGAA 
GTGGGTCAGT TCGTGCTGAC AGCCCTCACC CCGGAGATGT TGGCGGCTGA GGATGCGGAG 
TCGGAACCGG GCGAGCTTAT CTTTAACGTG ACTAGCTCTC TGGATCCGGA GACATCGGGC 
TACCTGATCA GCACCGACGA TCGCAACCTG CCCATTACTT CTTTCTACCA GCGGGACCTG 
ATGGAGCTGA GGATCGCGTA CCAGCCCCCG GCTAGTGAGT CCTGGCAGGA GCGGATCATT 
CAGCTGGAGA TGCATGTCAT TGACACCGAG GGGGCATCGT CCGATTACTT TGCGTTTATG 
ATCGTTGTAA AGCCCATGAA CACACTGGCC CCACTGGCGA CTCGCAACGC TGGCTTGCGG 
CTCCTAGAGG GTCAGTCTCG CCCTTTGTCC GGCCCAGGCT CCGGCATGAA CAACCTCGTT 
ATAAGCGATG AGGACAACCT GGACCAAGTC AGGATCAGTG TGATCGATGG TCTCCGGCAC 
GGCCAGTTAA TCCTAAGCCC CAGTGACAGC GGGACCAATA GTGGCCACAA GGACTTCTCC 
TTTAGCCCAC AGGAACTTCA CTCGGGGCTG GTGATTTATC AGCACGATGG CAGCGACACC 
TACAGCGACA ATCTGATCCT AAGGCTGGAC GATGGCAAGC ATCGGGTGGA GCTGCTGTAC 
CCCATCTCAG TGGCCCCTAC TGATGATGAG CCCCCAGTAC TGAATGCCAA CACCGGGCTG 
AACCTACGGG AGAAGGGCCT GGCTCTCATC TCCCCTTTCA TGCTCAGTGC CACAGACATC 
GACTCGGAAG ATTCCACCAT ACACTTTGTG CTGCAGGAAC CCCTCGGCCG ACTCGGGGAG 
ATGCTGCTGA GGCAGCCGGA TGAGCCGGAG GGGGAGGAGG GCTGGAAATA TCTGCCCCAG 
GAAGGGATTT ACGAGAAGGG GGTGATCGAG TGGTACCAGA AGGATATTCT GGATGGGAAA 
CTGCACTACA GGCACCTGGG TTCGCATAGT ACAACTACAG TTCTGGACCG AGTGCTGTTC 
GTGGTGCTGG ATGACCATGA CCCCCCAAAC CAGTCCAATG AGCAGGTATT TATGGTGCGC 
ATAGAGCCCC TTGATGACAT CCCCCCAGAA CTGTACCCCG GTACTACCCT GCACATGACT 
GTCAAGGAAT ACGAGGTGAC CCCCTTCAGG AAGAAGTACC TGCGCTACAC TGATCTGGAC 
ACAGACGACC GAGAGCTTAA ATATACCTTG CACAGCCACC CTAGGGATAC CGACCCCAAC 
CATCCTGCCC TGACTGGCCA GATTGTGCTG AGTGACAGCC TGGGGGTCGA GGTGAACCAA 
TTTACACAAG CCCAGATCAA CCATTTAAAG ATCTCATACA AGCCCCCAGA GAAAGAACTG 
GGCATAGTTC CCAGGGAGCT ACAGTTCAAC TACACAGTGG AGGATGCTGA GGGAAATTCG 
GTGCAAGGGA CATTCACTAT AGTGTTACAG CCAGTGGACA ATAAACCTCC GGAGATCACA 
AATACTGGTT TCACTGTCCC AGAGAGGGGT TCCTTCACTC TCACCAAAAA TGAACTGGAT 
GCCACAGATC AAGACACAGA CACAAGCAAC ATCATTTTCA GCTTGTCTGA AGCCCCTCAG 
CATGGGGAGT TACAGTACCA GGGTGTACCA ATGTCCTTGG GAGAGTCCTT TGCTTTAAGT 
GACATTTCAC TGGGTTGGGT ATCCTATGTT CATAGTGGAG AGGAGACCAC CAGCGACACA 
TGTTCCCTGG TAGTTAGTGA TGGTGTTCAC GAAGTTCCCA TTACAGTCCG AATTAAAGTA 
AAGCCAGTGG ATGACGAGGC ACCCATTTTG ATGCTTGCTG CAGGTCTTTT GGGGTCATCC 
ATTGAAGTCT TGGAGAGAGG AGAAACTGAG ATTACCAGCA GTGTTGTTCA GGGCACAGAT 
CCAGACACTG ATGATTTGAT GCTCACATTC ATTGTGGAGG AGCCTCCCAG AAAAGGGGAT 
ATATTGGTCA ATGGAGTTAC CGCTGAGAGG TTCTCTCAGC AAGACCTTCT CAATGGTGCT 
GTTACATATG TACATACATT TGGAGAGATT GGGCTTCAGA AGCAACATGA TTCCTTCAAT 
CTCACCCTCT CTGACCTCTC TGATGAGTGG GTGGTGGGAG GAAACAAGGT GCAGGGGGTG 
AGGGTGCAAG TAACCATCTT ACCAGTAGAC AGTGAAGCTC CAGTAGTAAC ATTAAAAGAA 
AAGTTCTCTG TTTTGGAAGG TGAGAAAAAT GTCATCACCT ATGAAAACCT GGACGCAGAT 
GACAAGGACA CCCCTAAAGA TGACATCTTG TGCACTATTA TTGTCCAGCC TACTTCTGGC 
TATGTAGAGA ACATTTCACC TGCCCCTGGC TCTGAAAAGT CAAGGTCTGG GACAGCAGTC 
AGTGCATTTA CAATAAGAGA TATCCGACTG GGTCACATTT ACTATGTTCA AAGCATCCAT 
CAGGGTGTAG AACCTCAGGA AGATAGATTC ACTTTTCACT GCTCTGATGG AATAAGCTCT 
TCACAGCGCC TTTTTTTCCC CATAGTGATC ATCCCAACCA ATGATGAGAA ACCAGAACTT 
TTCACACGGG AATTTGTAGT CATGGAAGGC ATGAGCCTGG TGATTGACAC CCCAATCCTT 
AATGCTGCAG ATGGAGACCA ACCACCGGAT GAACTTACCT TTATAATATC CAAGCCACCA 
CTACACGGTA GGGTGGTCCA ACAGCAAGCA ACAGGCACTG TCCCCATTCT GAATTTTACT 
TTAGAAGAAA TCAAAGAGAG CTCAAGCATT GTATATGAGC ATGACGATTC AGAAACCAAG 
GAGGACAGCT TTGAGATTAT CTTAACTGAT GGAGAACACA ATGTAATAAA GAAAGTCCTT 
ATTATGGTTA TTCCCGTGGA TGATGAGACT CCAAGAATGG CCATCAATGA TGGACTTGAA 
ATTGATATTG GAGAGTCAAA GGTAATCACA AACAGGGTTT TAAAGGCTAC AGACTTAGAT 
TCTGATGACA AGTCATTGGC TTATGTTATT CGGTTTGGCC CCAGCCAAGG TTTTCTGCAG 
CGCTTGGACA AACATGGGTC AGTGGTTGGT AATATTACCT TGGGAATGAA TTTTACTCAA 
GAGGACATTG ACAGAGGTTT AATTCGGTAC ACCCATGCTG GTCAGGAAGG CATACGGGAC 
CTTTTTAAAT TTGACATAAC AGATGGGATT AACCCATTCA TAGACAGATA CTTTTATATA 
AGCATTGGCA GCATTGATAT TGTTTTCCCA GATGTCATAA ACAAAGGAGT TACTCTCAAA 
GAAGGCGGAA AGGTGACACT TACTACAGAC CTTCTAAGTA CAAGTGACAT TAACAGTCCA 
GATGAAAATC TGCGCTTCAG TATTACAAGG GCCCCAAGCC GTGGGCACTT AGAGAGCACT 
GATGCCCCTG GTGTTCCCAT TACATCTTTT ACTCAGCTCC AACTAGCTGG GAATAAGGTC 
TATTACATCC ACACTTCAGA GGATGAGATG AAGATAGACA GTTTTGAGTT TGAAGTGACA 
GATGGTTATA ACCCAGTTTT TCGTACTTTT AGGGTCTCTA TTACTGATGT AGATAATAAA 
AAGCCAGTCT TGATGATAAA TGGACTAGTG GTCAATGAAG GAGAAAATAA GCTTATAACT 
CCCTTTGAGC TAAGTGTGGA GGACAGGGAC ACCCCAGATA ATTTGCTGCG ATTTACAATC 
ACTCAAGTGC CTGTTCATGG TCAGATTGTG TTCAACAACA GCCAGACAGT TACTACTTTC 
ACCAAGCAAG ACCTGAATGA GAATCTCATT AGCTACAAGC ATGATGGCAG TGAATCTGTA 
GAAGATAGCT TTTCCTTCAC AGTCACTGAT GGGACCCATG CAGAGTTTTT TGTACTGCCA 
GACACAACCT TTGAAAGCAA GAAACCCCAA ATGATGAAGA TCAGAATAAA TTCTTTGGAC 
AATGGTGTAC CCCAACTTAT TGTCAACAAG GGGGCCCCTA CTCTTAGAAG CCTACATCCA 
GGCCAACTTG GGTTCACGAT CACCAGCAAA TCCCTAAAGG CAGAAGATCG GGACAGCCCT 
CACAAGATGC TCAAATACAG AATCCTAGAT GGACCTAAAC ATGGGTTTTT AGTCAACTTG 
GACCATGGCA ATGACAGCAT CAGAACCTTC ACACAGGGCG ACATTGATGA CATGAAAATC 
TGCTATGTTC TGAAGGATGG TGACAATGCT ACAGGGGATA TTTTCCATTT CTCAGTAGAA 
GATATAGGGG GCAATAAACT AAGGAGTCAG CAATTTCGCC TGAACTGGGC TTGGATTTCA 
ATGGAAAAAA GCCATTATAC TGTAGACGAA GATTCAAAGT TTCTGGAAGT GACACTTAAA 
CGAAGAGGAT ACCTTGGAGA GACATCTTTT ATCAGCATCA CTACAAAAGA TGGCACAGCA 
ACCAAGGATA AAGACTTTAA AGGAAAAGCC CAGAAGCAGG TTCAGTTCAA CCCCGGGCAG 
ACTACAGCCA CATGGAAAGT GCGAATTCTT GCAGATAATG AATATGAGAA TTCTGAGACA 
TTTCATGTTA TCTTATCAGA ACCTGTTATG GCTGCCCTTG AGTTTCCAAT AGAAGCAACA 
ATTGAAATTG TAGATCCAGG TGATGAGTCC ACTGTTTACA TACCGCAAGC AGAATACAAG 
ATAGAGGAAG ATATTGGAGA ACTGATAATC CCTATTAAAA GATCAGGCGA CATAAGTCAA 
GAACTTATGG TAGTTTGTTC AACGCATCAA GGTACAGCTA CAGGCACTGT TCCCTCCTCT 
GTATTGTCCT ACTCTGACTA CATCACACGT GCAGATGGAC ACACAAGTAT TATTCGCTTT 
GACAAAGATG AGACTGAGAA AAACTGCCGT GTTATTATCA TTGATGACTC CCTGTATGAA 
GGAGACGAGA CATTTAATGT CAGCCTTAGT ATGCCCATGG GAGGACAGCT GGGAGAATTT 
CCAAGTACTA AAGTGGTTAT CCTTGCTGAT ACAGATGATG AGCCAGCCTT CTACTTTGCA 
GATACAGATT ATCATGTTGA TGAGAGTGCT GGGTATGTTG AAGTTCGTGT TTGGAGAACA 
GGGACAGATC TGACCAAAGC AGCCACGGTC ACTGTGCGTT CCAGGAAAAG CGAGCCTCTG 
TCTGCAGAAG CTGGAACAGA TTATGTGGGT ATCAGCAGAA ACCTTGATTT TGCCCCTGGT 
GTAACCATGC AGAGCTTCCG AGTAGTAATT CTTGATGATC TGGGGCAGCC TTCTCTGGAG 
GGACTTGAGA AATTTGAATT GGTTCTTCAA ATGCCCATGG GAGCAGTATT GGGGGAGCCC 
AGCAAAACAA CTGTGACCAT CAATGATACC ATCTCTGACT TACCTAAGGT TCAGTTTAAG 
GAGTCAGCTT ATTATGCCAA CGAGAAAGAT GGGCAAGTAT CTGCCGTTAT ATACCGCAGT 
GGTGATGTCA GCCATAAATC CTCAGTGCGT TGCTTCACCC GCCAAGGATC GGCGCAAGTC 
ACAATGGATT ATAACGAAAG GCCAAACACT GATGATTCTG CTGTCGTCTT CTTGCCTGGT 
GAGACTGAGA AGGCATGTGT GGTAACCCTG GAAGATGATA AAGTATATGA AGAAGAAGAA 
GAGTTCCGAT TGCTACTTGG AACACCCAAA AGTGACTCAC CTTTTGGAGC CTCAGTTGGA 
GAGTCTAAAG AAGCTCTCAT AAAGATTAAA GATGCGGAGG ACAAACCAAT TATCAGGTTT 
TCAGAAATCA AGTATAGTAT CCAGGAGCCC CAGGAGTCCG GTGAAATAGC TGTTGTGAAA 
ATCCCTGTGC TGCGAATTGG AGACAACTCC AAGGTGTCCA TTGTTAGAAT TCACACCAAA 
GATGGCTCTG CTACATCAGG AGAAGATTAT AACCCATTGT CTGAAGATAT TGAGTTTAAA 
GAAGGAGAAA CCGAACACTT TGTTGAAGTG GAGATATTAT ATGATGGAGT AAGAGAAATG 
CGTGAAGCAT TTACTATACA CCTGAAGCCT GATGAGAACA TGGTAGCTGA AATCCAGATG 
AATAAAGCCA TAGTGTATAT TGAAGAGATG GACAGTGTTG CTGATGTGAC TTTTCCAGCT 
GTACCACAGG TTGTCTCACT TTTGCTGTAT GATGACACCT TCAAAATCAA GGAAAATCCC 
AGTCCCCCCA CTGGTTACCC AGTTGTATGT GTAACGGCGT GTAATCCAAA ATACTATGAC 
TATGACAAAA CTGGATCAAT CTGTGCTGCT GAAAACATTA ATGATACTCT GACCCAGTAC 
CGCTGGCTCG TCAGCGCTCC AAGTGGAAGT GATGGTGTCA CCAGCCCCAT GCGTGAAGTT 
GACTCCAATA CGTTCTTCAC TGACACTAAG TCGATCACAT TGGACTCTAT CTACTTCCAT 
GCTGGATCAA GAATCCAATG TGCAGCCAGG GCAGTCAATG CAAATGGAGA CTCTGGTCTG 
GAATTGCTAA GCCCTATATT TAGTGTTAGC AGAGAGGAAG GTCTTTGTCA GCCTCGTATT 
CCTGGAACAG TGGGTGCTGA ACCATTCTCA GCAAAAATCC GTTACACAGG CCCTGAAGAT 
CCTGACTACC CTAATCTGAT AAAACTGACA GTAACAATGC CACATATGGA CGGTATGCTT 
CCAGTCATCT CAACCAGACA CATGTCCAAC TTTGAATTGA CGCTTAGTCC AGATGGCACA 
AGAGTTGGAA ACCACAAGTG CTCCAACCTT CTAGATTACA ATGAGATTCC AACCAAGCAC 
AGCTTTATTA CAGACAATAC CAAGAGCCCT GAAGTGATCG GAGAAACATC CCCCTACCAG 
TACAGCAATG CTCTTCGATC TGCTAGAACT CTACGCTTCT ATCGCAACCT CAATTTGGAG 
GCCTGTCTGT GGGAATTTAA CAGTTACTAT GACATGTCTG AACTTCTGAC AGATTGTGGC 
GGAACTATAG GGACAGATGG ACAAGTGCTG AATCTGGTCC AGTCCTATGT AACTCTACGG 
GTTCCCCTCT ATGTATCCTA TGTGTTTCAC TCGGCAGCAG CAGTTGGTGG GTGGCAGCAC 
TTTGACCTGC AGTCAGAGTT AAGGTTGACA TTTGTGTATG ATACTGCCAT CTTATGGAAC 
GAAGGAATAG GCAGCCCACC AGAATCTGAG CTACAGGGAT CCCTTTATCC AACGAGCATG 
CGCATTAATG ATGAAGGACG CTTGGTAGTC AATTTTAAGA CAGAGATTCG CTTTCGGGGA 
CAGTTTGTCA TTTCTCATCC AGGTACATCT CTTTCATCCA TGGTGATGTC TGCCGACCAT 
CCTGAACTCA CCTTTACATT GAGTCTGTTG CGCAGTGAGC CAAGCTTCAA TCAACCCGTT 
CAGCAATGGA GCTTCATCTC CGACTTTGCT GTACGTGATT ATTCTGGCAC ATATACAGTG 
AAGCTTATCC CATGTAGCAC CACCCCAAAC CAAGAATATT CTTTACCTGT TATCTGCAAT 
CCACGTGAAC CTATATCCTT CAATATCGAC ATTAGGTTCC AACAGGTTAG TGACCCAGTG 
GCAGCAGAAT TCAGTCTTAA CACCCAAATG TTGTTGCTGT CTAAAAAAGA ATTGTGGTTA 
TCAGATGGCT CAATGGGATT TGGCCAGGGA AGTGATGTTG CATTTACTGA AGGCTCTACA 
ATCTATGGGC GAATAATGGT GGATCCAGTT CAGAACCTTG GGGACTCTTT TATCTGCAAC 
ATTGAGAAAG TGTTTTTATG CACTGGTTCT GATGGTTATG TCCCCAAATA CAGCCCAGAA 
AACAAGGAAT ATGGATGCTT TGCGGACTCG CCAACATTAT TGTACAGATT TAAAATACTG 
GACAAAGCAC AGCCAGAAAC ACAGGCCAGG TTATTTGGAG ATATTCCATT CGAAGCCAGA 
TTAGCTATTG ATAACCCCAG TGCTCTGGCG CTGGTGAAGC AACCAGGTTC TGATGGTTTC 
AGCCTCTCAT CTGCGCCACT GTTTCAGGTG GCTTCTGGAC GGGAGTGGTT TATTCATACT 
ATTTACACAG TCCGCTCTAA GGAAAATGCA AACCGAGGGA TTGGAAAGCG TAGTGTGGAT 
TACCATCATG CCTTGAGTAG CGCTACTGAC CCCAGGGGGG AACTCACACT AAAGAGAAGC 
CGGAGAGCAT ATCCAGAAGA TTCCGCATTT GCACAGGACA TTGGTGCTGA TAAGAACAGA 
GGGACCAACA TTCAGCACAT TGCTTTGGAG CAGAGCAGCA AGGCCTCAGG TTACAATAAG 
GACATGTTGC CTGGTGGTGG CCAATATCAG AAGCCAGTCT TGGAAGTAAC AGGCCACAAC 
TCAGAGGGAC CTTTGGTGCC TGTTCTTGGA GGGGTGGGTG CTTTATTGCT CTTAATCTGC 
ACTGTGGTTA TCATTGGTCT GCTCGTGAAA CACCGGAGAC GGCAGCACTC TGAGAAGAAG 
TCTCCAACTA ATAGTTATCA TAACAGCCAT GAAACTATGA GTACAAGGCA CAGCAGCAGT 
GACAGCTCGG AAGTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

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frem3 cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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