myo5b cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

Gene
Clones
gRNAs

Summary

Gene Symbol	myo5b
Entrez Gene ID	100379760
Full Name	myosin VB
General protein information	Preferred Names myosin VB Names unconventional myosin-Vb
Gene Type	protein-coding
Organism	Xenopus tropicalis(tropical clawed frog)

mRNA and Protein(s)

mRNA	Protein	Name
XM_031894925.1	XP_031750785.1	unconventional myosin-Vb isoform X2
XM_031894924.1	XP_031750784.1	unconventional myosin-Vb isoform X1
XM_018090994.1	XP_017946483.1	unconventional myosin-Vb

Pathways from BioSystems

Homologies

Xenopus tropicalis (tropical clawed frog)	myo5b	XP_002936554.2
Oryza sativa (rice)	Os02g0777700	NP_001048291.2
Pan troglodytes (chimpanzee)	MYO5B	XP_003953333.1
Schizosaccharomyces pombe (fission yeast)	myo52	NP_588492.1
Macaca mulatta (Rhesus monkey)	MYO5B	XP_001090434.1
Canis lupus familiaris (dog)	MYO5B	XP_005623182.1
Bos taurus (cattle)	MYO5B	XP_002697867.2
Mus musculus (house mouse)	Myo5b	NP_963894.1
Danio rerio (zebrafish)	myo5b	NP_001155104.1
Arabidopsis thaliana (thale cress)	XI-I	NP_195046.3
Homo sapiens (human)	MYO5B	NP_001073936.1
Rattus norvegicus (Norway rat)	Myo5b	NP_058779.1
Danio rerio (zebrafish)	LOC100536519	XP_003200915.2

Gene Ontology

The following myo5b gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the myo5b cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OXa42397	XM_018090994.1 Latest version!	Xenopus tropicalis myosin VB (myo5b), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OXa49262	XM_031894924.1 Latest version!	Xenopus tropicalis myosin VB (myo5b), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OXa49263	XM_031894925.1 Latest version!	Xenopus tropicalis myosin VB (myo5b), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

myo5b ( XM_018090994.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_017946483.1 Xenopus tropicalis myosin VB (myo5b), mRNA.

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CloneID	OXa42397
Clone ID Related Accession (Same CDS sequence)	XM_018090994.1
Accession Version	XM_018090994.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5475bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1473177600000
Organism	Xenopus tropicalis(tropical clawed frog)
Product	unconventional myosin-Vb
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## ab initio :: 1% of CDS bases ##RefSeq-Attributes-END##

ATGCAGTTTT GTTTAGCCAT TACAGATAGT AATTCCAGAG CCACGTTAAT ATATGGCATT 
TGTGTCTTTC AGTACACCCG GGTGTGGATA CCCGATCCGG AGGATGTCTG GAGATCAGCA 
GAAATTATCA AGGACTATAA AGAAGGGGAT AAAAGTCTGC AGTTAAAACT AGAAGATGAG 
TCTGTCATAC AGCACCGTGT GGATCCAAAC AACTTACCGC ACCTCCGCAA CCCAGATATC 
CTAGTAGGAG AGAATGACCT GACGGCTTTG AGCTATCTCC ATGAACCAGC TGTGCTCCAT 
AACCTAAAAG TCCGCTTCCT GGAATCAAAT CACATTTATA CTTATTGCGG AATTGTACTT 
GTTGCCATAA ACCCATACCA GCAGTTACCA GTCTATGGAG AAGATGTCAT TTACGCATAC 
AGCGGCCAAA ATATGGGAGA CATGGACCCC CACATATTTG CAGTGGCTGA AGAGGCCTAT 
AAGCAAATGG CCAGGGATGA AAAGAACCAG TCTATCATCG TAAGTGGAGA GTCCGGAGCA 
GGGAAAACTG TCTCTGCCAA ATATGCCATG CGCTACTTTG CCACAGTGGG CGGCTCAGCA 
AGTGAAGCTA ATGTGGAAGA GAAAGTATTG GCTTCTAGTC CCATCATGGA GGCTATTGGG 
AATGCCAAAA CAACCCGGAA TGACAACAGC AGCCGATTTG GAAAGTACAT TCAGATTGGG 
TTTGACAAAC GATATCATAT TATCGGTGCC AACATGAGGA CTTACCTTCT TGAGAAATCA 
CGTGTTGTTT TCCAGGCTGA TATGGAAAGG AACTACCATA TTTTCTACCA GCTCTGTGCC 
TCTGCCAGCC TGCCGGAGTT CTCCGAGCTG TCATTAGCTG AAGCTGAGGA GTTCTTTTAC 
ACCAACCAGG GAGGCGACCC CGTTATTGAT GGAGTGGATG ATGCTGAAGA CTTTGAGAAG 
ACGAGACAGG CTTTCACACT TTTGGGAGTG AAGGAGACTC ATCAAATGGG CATCTTTAAG 
ATTGTGGCTT CCATTCTGCA CCTTGGAAAT GTAGCCATCC AGACGGAGCG GGAAGGGGAA 
TCGTGTAACC TGTCGAAAGG GGATAAACAC CTGACTCATT TCTGCAGTTT GCTTGGGCTG 
GAGCAGGACC AGATGCAACA CTGGCTGTGC CACCGGAAAC TTGTCACCAC CTCCGAAACG 
TACGTCAAGA CCATGTCTGC GGAGCAGGCT GTAAATGCCA GGAACGCCTT GGCCAAGCAC 
ATCTACGCCC AGCTCTTCAA CTGGATAGTG CAGCACGTGA ACAAGGCCCT GCACACCACC 
ATCAAACAGC ACTCCTTTAT TGGGGTCCTC GACATATACG GGTTTGAAAC CTTTGAAATT 
AACAGCTTTG AGCAATTTTG CATCAATTAC GCCAATGAGA AGCTGCAACA GCAATTTAAT 
TCGCACGTCT TTAAACTGGA ACAAGAAGAA TACATGAAAG AGCAGATTCC ATGGACCCTG 
ATAGACTTCT ACGACAACCA ACCTTGTATT GACCTGATTG AGGCAAAATT AGGCATTTTG 
GACCTTCTAG ATGAAGAGTG CAAGGTCCCC AAAGGAACTG ATCAGAACTG GGCACAGAAG 
TTATATGACC GGCATGGAAG CTCCCAACAC TTCAAGAAAC CACGAATGTC TAATGTGGCA 
TTTATTGTGG TGCATTTTGC TGATCAGGTG GAGTATCAAT GTGACGGATT CCTGGAGAAG 
AACAGGGACA CTGTGTATGA GGAACAGATT AACATACTAA AGGCCTCAAA GTATCCGCTG 
GTTGCTGACC TGTTTCAAAA TGAAAAAGAC ACTGTTCCAC CTACTCCTAC CGGGAAGAGT 
AAAATCAGCG TTCGCCCTGC AAAACCTGCT CTGAAAAGTG CTAATAAAGA ACATAAGAAA 
ACAGTGGGAC ATCAGTTCCG GAACTCTCTG CATCTGCTGA TGGAAACCTT GAATGCCACA 
ACTCCTCACT ATGTGCGCTG CATTAAGCCC AATGACTATA AGGAGTCTTT CAGCTTCGAT 
CCAAAGAGAG CTGTGCAGCA GCTGAGGGCA TGTGGCGTGC TGGAGACCAT CCGGATTAGT 
GCTGCCGGCT ATCCATCCAG GTGGACCTAC CATGACTTCT TCAACAGGTA CCGAGTCCTA 
ATGAAAAAGA AAGATATTTC TCTGGGAGAC AAGAAGGTGA TCTGTAAGAA TGTCTTAGAG 
AACCTTATCA AGGACCCTGA CAAGTTCCAG TTTGGACGAA CAAAGATCTT TTTCCGTGCT 
GGCCAAGTGG CCTACTTAGA AAAACTGCGT GCAGACAAGT TCCGTGCCGC TACCATTATG 
ATTCAGAAGA CTGTGCGGGG CTGGCTTCAG AGGGTGAAAT ACAGAAGGAT GAAGAAGGCC 
GCGGTTACCA TCCAGAGATA TACACGTGGG CACCTTGCCC GAAGGTTGGC TCTGCACCTC 
CGAATGACGA AGGCTGCCAT CACCTTCCAG AAGCAATTCC GAATGATTCG AGTGTATCGC 
GGCTTCCAGA AGACTCGCAA GGCAGCCGTC ACTATCCAGT CCTACACTCG GGGAATGTTT 
GTCAGGAGAG CTTATCGGCA GCTTCTGGAG CAGCATAAAG CCGCCATCAT CCAGAAACAC 
TGGCGCGGAT GGAGGGCACG CAAACTGTTT AGCAAATTCC GCAGTGCCGC TGTCGTTATT 
CAGTGTTACT TCCGCCGCAT GAAAGCCCGA CGGGAGCTCA AACAGTTGAA GATCGAAGCC 
CGAACAGCAG AGCATTTTAA GAAGCTCAGT GTGGGCATGG AGAACAAGGT GGTTCAGCTG 
CAGAGAAAGC TTGATGAGCA GAACAAAGAG CAGAAAGACC TGAAGGAGCA ACTGGTATCT 
GCTACTTCAA CTCATGCTGT CGAGGTTGAG AAACTTCAGA AGGAACTCGA GAAGCAGAGA 
CAGGCTCAGC AGGATGAGAA CCAACTCACC AGCTTGCAGC AGGAACTGGA GGCTCTGAGA 
GAGGAACTGG CCAAAGCGTA TAGTGAGAAG AAGGTTGTAG AAGACACTCT TATGAATGAG 
AAAGAGCAAC TGCTGCAGCG CGTCTCAGAG CTGGAAGAGG AGAATACTAA CTTGAAAGAA 
GAAAAAGAAG AACTCAGTAA TAAAATCATT CTACGATCTG AAGATGAATT TACCCAGAGT 
ACAGTTAAGG AGAACCTGGA GATCAAGAAA GATCTTGAGG AGGAAAGGTC CCGGCACCAG 
AATTTGGTAA AGGAATATAC AAGGTTGGAG CAGAGATATG ACAATCTGAA GGAGGAGGTG 
AACATTATTA AGCAACAAAT GCCGGGGCAC AGGAGGAATC CGTCCAACCA GAGCAGCTTG 
GAATCTGACT CTAATTACCC TTCCATATCT ACCTCTGAGA TTGGGGACAC AGAGGATACC 
ATTAAGGAGG TGGAGGAGGC CGGGCTGGAA AAGGCTGCCA TGGACATGTC TCTGTTCCTG 
AAGCTGCAGA AGAGAGTGAG GGAGCTTGAG CTGGAGAGGA AGAAGCTGCA GAGCCAACTG 
GACAAAAAAG AGCAGCAAGA AAATAAAAAA TCCCAGGTTA TAGACACGAC CAATGAGGTG 
ATTGCTGACC GCGACACTGA TATCGCCTAC AACAATCTGA AGCGGCAAGA ACTTGAGTCT 
GAAAACAAGA AGCTGAAAAA TGACTTGAAT GAGATGAGGA AGACGATAGC AGATGGAGCA 
GTAGATGGTT CGAGTGAAGA GGCCAGAATT GCTATGTTAC TCAACCAACT GAAGTCGGCC 
CACGAGGAGC TGGATGTGCG GAAGGAAGAA GTTCTTATGC TCAGAACCCA GATTGTAAAG 
GCAGCTCAGC AAAAAGAATC AGAGAGGATT ATGAGTTCAG AAGCCAGTTG GCCAAACAGC 
AACAAGGAAA TGGATGAAGA CGATGCCATT GAAGCCTATC ATGGAGTCTG TGAGACAAAC 
AGGCTTCTGG AGGCTCAGCT TCAGGCACAA AGCAGGCAGC ACGATGAGGA GGTGGAGATA 
CTGACCTCTC ACATTGACTC CCTAAAGGAA GAGTTGGAGA AGCAGCAGCA GGTTCTACTC 
CAAGCTCTTC AGCTCTCCCC AGAGGCTCAG GTGGAGTTTG GGCTTCAGCA TGAAATTACC 
CGCCTCACTA ATGAGAACCT GGACCTTAAA GAATTATTGG AGAAATCAGA AAAGAATGAG 
AAAAAATTGA AGAAACAGCT AAAAACGTAC ATGAAGAAAG GACAGGATTC TGAAGATGTA 
GTGCAGACGG AGAGGAAACC TGAGCTCACC CGTCAAGTGA CAGTGCAGCG AATTGAGAAG 
GATTTCCAAG GAATGCTGGA GTATTACAAG GAGGAAGAAC CGCTGCTCGT GAGAAATCTC 
ATTATAGACG TGAAGCCTGA GCAGATGTCT TCCATGGTTC CTTGTCTGCC TGCGTATATC 
CTCTTCATGT GCATCAGGCA CGCCGACTAC ATCAACGATG ATCAGAAGGT CCACTCCCTG 
CTCACCTCCA CCATCAACGG GATAAAGAAA GTTCTAAAGA AGCATCACAG TGAATTCCAG 
ATTACATCTT TCTGGCTGTC CAACTCCAGC CGGCTGCTCC ATTGCCTGAA ACAGTATAGT 
GGGGACGAGG GCTTCATGAC CAGCAATAGC CCCAAGCAGA ACGAGCATTG CCTGAGGAAC 
TTTGATCTGA CAGAATACCG CCAGGTCCTC AGCGACTTGT CTATCCAGAT CTACCAGCAG 
ATGATAAAGA TTGCGGAAAC GCATATGCAG CCAATGATAG TGTCTGCCAT GTTGGAAACA 
GAAAGCATCC AAGGCCTCTC AGGAGTGAAG CAAACTGGGT ACAGGAAGAG GACCTCCAGC 
ATGGCGGATG GGGATAATTC CTACAGCCTA GAAGCTGTCA TTCGCCAGCT GAACACGTTT 
AACAGCATCA TGTGTGACCA TGGACTGGAT CCCGAAATCA TTCAGCAAGT CTTTAAGCAG 
CTCTTCTACA TGATAAATGC TGTAACGCTG AATAATCTGC TGCTGAGGAA GGACGTTTGC 
TCATGGAGCA CCGGCATGCA GCTAAGGTAT AATATCAGTC AGTTAGAAGA ATGGCTGCGA 
GGGAAGAATT TGCATCCGAG TGGCGCTGCT CAGACTATGG AGCCGCTGAT CCAAGCAGCA 
CAGTTACTGC AACTCAAAAA GAAGACAGAG GAGGATGCAG AGGCCATATG CTCTTTGTGC 
ACAGCCCTGA CGACCCAGCA GATTGTGAAG ATACTGAATC TGTACACACC CGTGAATGAG 
TTTGAAGAGC GAGTAACCGT GTCCTTTATC AGAATCATTC AGGCTCAGCT GCAAGAGCGG 
AACGATCCTC CCCAGCTGCT AATGGACTCT AAGTACATGT TCCCAGTCCT GTTCCCATTC 
AGCCCCTCGG CCATCACTTT GGACACCATT CAGATCCCTC CTTCCCTCAA ACTGGATTTC 
CTCATGAAGG TCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017946483.1
CDS	1..5475
Translation	MQFCLAITDSNSRATLIYGICVFQYTRVWIPDPEDVWRSAEIIKDYKEGDKSLQLKLEDESVIQHRVDPNNLPHLRNPDILVGENDLTALSYLHEPAVLHNLKVRFLESNHIYTYCGIVLVAINPYQQLPVYGEDVIYAYSGQNMGDMDPHIFAVAEEAYKQMARDEKNQSIIVSGESGAGKTVSAKYAMRYFATVGGSASEANVEEKVLASSPIMEAIGNAKTTRNDNSSRFGKYIQIGFDKRYHIIGANMRTYLLEKSRVVFQADMERNYHIFYQLCASASLPEFSELSLAEAEEFFYTNQGGDPVIDGVDDAEDFEKTRQAFTLLGVKETHQMGIFKIVASILHLGNVAIQTEREGESCNLSKGDKHLTHFCSLLGLEQDQMQHWLCHRKLVTTSETYVKTMSAEQAVNARNALAKHIYAQLFNWIVQHVNKALHTTIKQHSFIGVLDIYGFETFEINSFEQFCINYANEKLQQQFNSHVFKLEQEEYMKEQIPWTLIDFYDNQPCIDLIEAKLGILDLLDEECKVPKGTDQNWAQKLYDRHGSSQHFKKPRMSNVAFIVVHFADQVEYQCDGFLEKNRDTVYEEQINILKASKYPLVADLFQNEKDTVPPTPTGKSKISVRPAKPALKSANKEHKKTVGHQFRNSLHLLMETLNATTPHYVRCIKPNDYKESFSFDPKRAVQQLRACGVLETIRISAAGYPSRWTYHDFFNRYRVLMKKKDISLGDKKVICKNVLENLIKDPDKFQFGRTKIFFRAGQVAYLEKLRADKFRAATIMIQKTVRGWLQRVKYRRMKKAAVTIQRYTRGHLARRLALHLRMTKAAITFQKQFRMIRVYRGFQKTRKAAVTIQSYTRGMFVRRAYRQLLEQHKAAIIQKHWRGWRARKLFSKFRSAAVVIQCYFRRMKARRELKQLKIEARTAEHFKKLSVGMENKVVQLQRKLDEQNKEQKDLKEQLVSATSTHAVEVEKLQKELEKQRQAQQDENQLTSLQQELEALREELAKAYSEKKVVEDTLMNEKEQLLQRVSELEEENTNLKEEKEELSNKIILRSEDEFTQSTVKENLEIKKDLEEERSRHQNLVKEYTRLEQRYDNLKEEVNIIKQQMPGHRRNPSNQSSLESDSNYPSISTSEIGDTEDTIKEVEEAGLEKAAMDMSLFLKLQKRVRELELERKKLQSQLDKKEQQENKKSQVIDTTNEVIADRDTDIAYNNLKRQELESENKKLKNDLNEMRKTIADGAVDGSSEEARIAMLLNQLKSAHEELDVRKEEVLMLRTQIVKAAQQKESERIMSSEASWPNSNKEMDEDDAIEAYHGVCETNRLLEAQLQAQSRQHDEEVEILTSHIDSLKEELEKQQQVLLQALQLSPEAQVEFGLQHEITRLTNENLDLKELLEKSEKNEKKLKKQLKTYMKKGQDSEDVVQTERKPELTRQVTVQRIEKDFQGMLEYYKEEEPLLVRNLIIDVKPEQMSSMVPCLPAYILFMCIRHADYINDDQKVHSLLTSTINGIKKVLKKHHSEFQITSFWLSNSSRLLHCLKQYSGDEGFMTSNSPKQNEHCLRNFDLTEYRQVLSDLSIQIYQQMIKIAETHMQPMIVSAMLETESIQGLSGVKQTGYRKRTSSMADGDNSYSLEAVIRQLNTFNSIMCDHGLDPEIIQQVFKQLFYMINAVTLNNLLLRKDVCSWSTGMQLRYNISQLEEWLRGKNLHPSGAAQTMEPLIQAAQLLQLKKKTEEDAEAICSLCTALTTQQIVKILNLYTPVNEFEERVTVSFIRIIQAQLQERNDPPQLLMDSKYMFPVLFPFSPSAITLDTIQIPPSLKLDFLMKV

Target ORF information:

RefSeq Version	XM_018090994.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis myosin VB (myo5b), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_018090994.1

ORF Insert Sequence:

ATGCAGTTTT GTTTAGCCAT TACAGATAGT AATTCCAGAG CCACGTTAAT ATATGGCATT 
TGTGTCTTTC AGTACACCCG GGTGTGGATA CCCGATCCGG AGGATGTCTG GAGATCAGCA 
GAAATTATCA AGGACTATAA AGAAGGGGAT AAAAGTCTGC AGTTAAAACT AGAAGATGAG 
TCTGTCATAC AGCACCGTGT GGATCCAAAC AACTTACCGC ACCTCCGCAA CCCAGATATC 
CTAGTAGGAG AGAATGACCT GACGGCTTTG AGCTATCTCC ATGAACCAGC TGTGCTCCAT 
AACCTAAAAG TCCGCTTCCT GGAATCAAAT CACATTTATA CTTATTGCGG AATTGTACTT 
GTTGCCATAA ACCCATACCA GCAGTTACCA GTCTATGGAG AAGATGTCAT TTACGCATAC 
AGCGGCCAAA ATATGGGAGA CATGGACCCC CACATATTTG CAGTGGCTGA AGAGGCCTAT 
AAGCAAATGG CCAGGGATGA AAAGAACCAG TCTATCATCG TAAGTGGAGA GTCCGGAGCA 
GGGAAAACTG TCTCTGCCAA ATATGCCATG CGCTACTTTG CCACAGTGGG CGGCTCAGCA 
AGTGAAGCTA ATGTGGAAGA GAAAGTATTG GCTTCTAGTC CCATCATGGA GGCTATTGGG 
AATGCCAAAA CAACCCGGAA TGACAACAGC AGCCGATTTG GAAAGTACAT TCAGATTGGG 
TTTGACAAAC GATATCATAT TATCGGTGCC AACATGAGGA CTTACCTTCT TGAGAAATCA 
CGTGTTGTTT TCCAGGCTGA TATGGAAAGG AACTACCATA TTTTCTACCA GCTCTGTGCC 
TCTGCCAGCC TGCCGGAGTT CTCCGAGCTG TCATTAGCTG AAGCTGAGGA GTTCTTTTAC 
ACCAACCAGG GAGGCGACCC CGTTATTGAT GGAGTGGATG ATGCTGAAGA CTTTGAGAAG 
ACGAGACAGG CTTTCACACT TTTGGGAGTG AAGGAGACTC ATCAAATGGG CATCTTTAAG 
ATTGTGGCTT CCATTCTGCA CCTTGGAAAT GTAGCCATCC AGACGGAGCG GGAAGGGGAA 
TCGTGTAACC TGTCGAAAGG GGATAAACAC CTGACTCATT TCTGCAGTTT GCTTGGGCTG 
GAGCAGGACC AGATGCAACA CTGGCTGTGC CACCGGAAAC TTGTCACCAC CTCCGAAACG 
TACGTCAAGA CCATGTCTGC GGAGCAGGCT GTAAATGCCA GGAACGCCTT GGCCAAGCAC 
ATCTACGCCC AGCTCTTCAA CTGGATAGTG CAGCACGTGA ACAAGGCCCT GCACACCACC 
ATCAAACAGC ACTCCTTTAT TGGGGTCCTC GACATATACG GGTTTGAAAC CTTTGAAATT 
AACAGCTTTG AGCAATTTTG CATCAATTAC GCCAATGAGA AGCTGCAACA GCAATTTAAT 
TCGCACGTCT TTAAACTGGA ACAAGAAGAA TACATGAAAG AGCAGATTCC ATGGACCCTG 
ATAGACTTCT ACGACAACCA ACCTTGTATT GACCTGATTG AGGCAAAATT AGGCATTTTG 
GACCTTCTAG ATGAAGAGTG CAAGGTCCCC AAAGGAACTG ATCAGAACTG GGCACAGAAG 
TTATATGACC GGCATGGAAG CTCCCAACAC TTCAAGAAAC CACGAATGTC TAATGTGGCA 
TTTATTGTGG TGCATTTTGC TGATCAGGTG GAGTATCAAT GTGACGGATT CCTGGAGAAG 
AACAGGGACA CTGTGTATGA GGAACAGATT AACATACTAA AGGCCTCAAA GTATCCGCTG 
GTTGCTGACC TGTTTCAAAA TGAAAAAGAC ACTGTTCCAC CTACTCCTAC CGGGAAGAGT 
AAAATCAGCG TTCGCCCTGC AAAACCTGCT CTGAAAAGTG CTAATAAAGA ACATAAGAAA 
ACAGTGGGAC ATCAGTTCCG GAACTCTCTG CATCTGCTGA TGGAAACCTT GAATGCCACA 
ACTCCTCACT ATGTGCGCTG CATTAAGCCC AATGACTATA AGGAGTCTTT CAGCTTCGAT 
CCAAAGAGAG CTGTGCAGCA GCTGAGGGCA TGTGGCGTGC TGGAGACCAT CCGGATTAGT 
GCTGCCGGCT ATCCATCCAG GTGGACCTAC CATGACTTCT TCAACAGGTA CCGAGTCCTA 
ATGAAAAAGA AAGATATTTC TCTGGGAGAC AAGAAGGTGA TCTGTAAGAA TGTCTTAGAG 
AACCTTATCA AGGACCCTGA CAAGTTCCAG TTTGGACGAA CAAAGATCTT TTTCCGTGCT 
GGCCAAGTGG CCTACTTAGA AAAACTGCGT GCAGACAAGT TCCGTGCCGC TACCATTATG 
ATTCAGAAGA CTGTGCGGGG CTGGCTTCAG AGGGTGAAAT ACAGAAGGAT GAAGAAGGCC 
GCGGTTACCA TCCAGAGATA TACACGTGGG CACCTTGCCC GAAGGTTGGC TCTGCACCTC 
CGAATGACGA AGGCTGCCAT CACCTTCCAG AAGCAATTCC GAATGATTCG AGTGTATCGC 
GGCTTCCAGA AGACTCGCAA GGCAGCCGTC ACTATCCAGT CCTACACTCG GGGAATGTTT 
GTCAGGAGAG CTTATCGGCA GCTTCTGGAG CAGCATAAAG CCGCCATCAT CCAGAAACAC 
TGGCGCGGAT GGAGGGCACG CAAACTGTTT AGCAAATTCC GCAGTGCCGC TGTCGTTATT 
CAGTGTTACT TCCGCCGCAT GAAAGCCCGA CGGGAGCTCA AACAGTTGAA GATCGAAGCC 
CGAACAGCAG AGCATTTTAA GAAGCTCAGT GTGGGCATGG AGAACAAGGT GGTTCAGCTG 
CAGAGAAAGC TTGATGAGCA GAACAAAGAG CAGAAAGACC TGAAGGAGCA ACTGGTATCT 
GCTACTTCAA CTCATGCTGT CGAGGTTGAG AAACTTCAGA AGGAACTCGA GAAGCAGAGA 
CAGGCTCAGC AGGATGAGAA CCAACTCACC AGCTTGCAGC AGGAACTGGA GGCTCTGAGA 
GAGGAACTGG CCAAAGCGTA TAGTGAGAAG AAGGTTGTAG AAGACACTCT TATGAATGAG 
AAAGAGCAAC TGCTGCAGCG CGTCTCAGAG CTGGAAGAGG AGAATACTAA CTTGAAAGAA 
GAAAAAGAAG AACTCAGTAA TAAAATCATT CTACGATCTG AAGATGAATT TACCCAGAGT 
ACAGTTAAGG AGAACCTGGA GATCAAGAAA GATCTTGAGG AGGAAAGGTC CCGGCACCAG 
AATTTGGTAA AGGAATATAC AAGGTTGGAG CAGAGATATG ACAATCTGAA GGAGGAGGTG 
AACATTATTA AGCAACAAAT GCCGGGGCAC AGGAGGAATC CGTCCAACCA GAGCAGCTTG 
GAATCTGACT CTAATTACCC TTCCATATCT ACCTCTGAGA TTGGGGACAC AGAGGATACC 
ATTAAGGAGG TGGAGGAGGC CGGGCTGGAA AAGGCTGCCA TGGACATGTC TCTGTTCCTG 
AAGCTGCAGA AGAGAGTGAG GGAGCTTGAG CTGGAGAGGA AGAAGCTGCA GAGCCAACTG 
GACAAAAAAG AGCAGCAAGA AAATAAAAAA TCCCAGGTTA TAGACACGAC CAATGAGGTG 
ATTGCTGACC GCGACACTGA TATCGCCTAC AACAATCTGA AGCGGCAAGA ACTTGAGTCT 
GAAAACAAGA AGCTGAAAAA TGACTTGAAT GAGATGAGGA AGACGATAGC AGATGGAGCA 
GTAGATGGTT CGAGTGAAGA GGCCAGAATT GCTATGTTAC TCAACCAACT GAAGTCGGCC 
CACGAGGAGC TGGATGTGCG GAAGGAAGAA GTTCTTATGC TCAGAACCCA GATTGTAAAG 
GCAGCTCAGC AAAAAGAATC AGAGAGGATT ATGAGTTCAG AAGCCAGTTG GCCAAACAGC 
AACAAGGAAA TGGATGAAGA CGATGCCATT GAAGCCTATC ATGGAGTCTG TGAGACAAAC 
AGGCTTCTGG AGGCTCAGCT TCAGGCACAA AGCAGGCAGC ACGATGAGGA GGTGGAGATA 
CTGACCTCTC ACATTGACTC CCTAAAGGAA GAGTTGGAGA AGCAGCAGCA GGTTCTACTC 
CAAGCTCTTC AGCTCTCCCC AGAGGCTCAG GTGGAGTTTG GGCTTCAGCA TGAAATTACC 
CGCCTCACTA ATGAGAACCT GGACCTTAAA GAATTATTGG AGAAATCAGA AAAGAATGAG 
AAAAAATTGA AGAAACAGCT AAAAACGTAC ATGAAGAAAG GACAGGATTC TGAAGATGTA 
GTGCAGACGG AGAGGAAACC TGAGCTCACC CGTCAAGTGA CAGTGCAGCG AATTGAGAAG 
GATTTCCAAG GAATGCTGGA GTATTACAAG GAGGAAGAAC CGCTGCTCGT GAGAAATCTC 
ATTATAGACG TGAAGCCTGA GCAGATGTCT TCCATGGTTC CTTGTCTGCC TGCGTATATC 
CTCTTCATGT GCATCAGGCA CGCCGACTAC ATCAACGATG ATCAGAAGGT CCACTCCCTG 
CTCACCTCCA CCATCAACGG GATAAAGAAA GTTCTAAAGA AGCATCACAG TGAATTCCAG 
ATTACATCTT TCTGGCTGTC CAACTCCAGC CGGCTGCTCC ATTGCCTGAA ACAGTATAGT 
GGGGACGAGG GCTTCATGAC CAGCAATAGC CCCAAGCAGA ACGAGCATTG CCTGAGGAAC 
TTTGATCTGA CAGAATACCG CCAGGTCCTC AGCGACTTGT CTATCCAGAT CTACCAGCAG 
ATGATAAAGA TTGCGGAAAC GCATATGCAG CCAATGATAG TGTCTGCCAT GTTGGAAACA 
GAAAGCATCC AAGGCCTCTC AGGAGTGAAG CAAACTGGGT ACAGGAAGAG GACCTCCAGC 
ATGGCGGATG GGGATAATTC CTACAGCCTA GAAGCTGTCA TTCGCCAGCT GAACACGTTT 
AACAGCATCA TGTGTGACCA TGGACTGGAT CCCGAAATCA TTCAGCAAGT CTTTAAGCAG 
CTCTTCTACA TGATAAATGC TGTAACGCTG AATAATCTGC TGCTGAGGAA GGACGTTTGC 
TCATGGAGCA CCGGCATGCA GCTAAGGTAT AATATCAGTC AGTTAGAAGA ATGGCTGCGA 
GGGAAGAATT TGCATCCGAG TGGCGCTGCT CAGACTATGG AGCCGCTGAT CCAAGCAGCA 
CAGTTACTGC AACTCAAAAA GAAGACAGAG GAGGATGCAG AGGCCATATG CTCTTTGTGC 
ACAGCCCTGA CGACCCAGCA GATTGTGAAG ATACTGAATC TGTACACACC CGTGAATGAG 
TTTGAAGAGC GAGTAACCGT GTCCTTTATC AGAATCATTC AGGCTCAGCT GCAAGAGCGG 
AACGATCCTC CCCAGCTGCT AATGGACTCT AAGTACATGT TCCCAGTCCT GTTCCCATTC 
AGCCCCTCGG CCATCACTTT GGACACCATT CAGATCCCTC CTTCCCTCAA ACTGGATTTC 
CTCATGAAGG TCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

myo5b ( XM_031894924.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_031750784.1 Xenopus tropicalis myosin VB (myo5b), transcript variant X1, mRNA.

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CloneID	OXa49262
Clone ID Related Accession (Same CDS sequence)	XM_031894924.1
Accession Version	XM_031894924.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5430bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1576512000000
Organism	Xenopus tropicalis(tropical clawed frog)
Product	unconventional myosin-Vb isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCAGTGT ATGAGCTTTA CTCCCAGTAC ACCCGGGTGT GGATACCCGA TCCGGAGGAT 
GTCTGGAGAT CAGCAGAAAT TATCAAGGAC TATAAAGAAG GGGATAAAAG TCTGCAGTTA 
AAACTAGAAG ATGAGTCTGT CATACAGCAC CGTGTGGATC CAAACAACTT ACCGCACCTC 
CGCAACCCAG ATATCCTAGT AGGAGAGAAT GACCTGACGG CTTTGAGCTA TCTCCATGAA 
CCAGCTGTGC TCCATAACCT AAAAGTCCGC TTCCTGGAAT CAAATCACAT TTATACTTAT 
TGCGGAATTG TACTTGTTGC CATAAACCCA TACCAGCAGT TACCAGTCTA TGGAGAAGAT 
GTCATTTACG CATACAGCGG CCAAAATATG GGAGACATGG ACCCCCACAT ATTTGCAGTG 
GCTGAAGAGG CCTATAAGCA AATGGCCAGG GATGAAAAGA ACCAGTCTAT CATCGTAAGT 
GGAGAGTCCG GAGCAGGGAA AACTGTCTCT GCCAAATATG CCATGCGCTA CTTTGCCACA 
GTGGGCGGCT CAGCAAGTGA AGCTAATGTG GAAGAGAAAG TATTGGCTTC TAGTCCCATC 
ATGGAGGCTA TTGGGAATGC CAAAACAACC CGGAATGACA ACAGCAGCCG ATTTGGAAAG 
TACATTCAGA TTGGGTTTGA CAAACGATAT CATATTATCG GTGCCAACAT GAGGACTTAC 
CTTCTTGAGA AATCACGTGT TGTTTTCCAG GCTGATATGG AAAGGAACTA CCATATTTTC 
TACCAGCTCT GTGCCTCTGC CAGCCTGCCG GAGTTCTCCG AGCTGTCATT AGCTGAAGCT 
GAGGAGTTCT TTTACACCAA CCAGGGAGGC GACCCCGTTA TTGATGGAGT GGATGATGCT 
GAAGACTTTG AGAAGACGAG ACAGGCTTTC ACACTTTTGG GAGTGAAGGA GACTCATCAA 
ATGGGCATCT TTAAGATTGT GGCTTCCATT CTGCACCTTG GAAATGTAGC CATCCAGACG 
GAGCGGGAAG GGGAATCGTG TAACCTGTCG AAAGGGGATA AACACCTGAC TCATTTCTGC 
AGTTTGCTTG GGCTGGAGCA GGACCAGATG CAACACTGGC TGTGCCACCG GAAACTTGTC 
ACCACCTCCG AAACGTACGT CAAGACCATG TCTGCGGAGC AGGCTGTAAA TGCCAGGAAC 
GCCTTGGCCA AGCACATCTA CGCCCAGCTC TTCAACTGGA TAGTGCAGCA CGTGAACAAG 
GCCCTGCACA CCACCATCAA ACAGCACTCC TTTATTGGGG TCCTCGACAT ATACGGGTTT 
GAAACCTTTG AAATTAACAG CTTTGAGCAA TTTTGCATCA ATTACGCCAA TGAGAAGCTG 
CAACAGCAAT TTAATTCGCA CGTCTTTAAA CTGGAACAAG AAGAATACAT GAAAGAGCAG 
ATTCCATGGA CCCTGATAGA CTTCTACGAC AACCAACCTT GTATTGACCT GATTGAGGCA 
AAATTAGGCA TTTTGGACCT TCTAGATGAA GAGTGCAAGG TCCCCAAAGG AACTGATCAG 
AACTGGGCAC AGAAGTTATA TGACCGGCAT GGAAGCTCCC AACACTTCAA GAAACCACGA 
ATGTCTAATG TGGCATTTAT TGTGGTGCAT TTTGCTGATC AGGTGGAGTA TCAATGTGAC 
GGATTCCTGG AGAAGAACAG GGACACTGTG TATGAGGAAC AGATTAACAT ACTAAAGGCC 
TCAAAGTATC CGCTGGTTGC TGACCTGTTT CAAAATGAAA AAGACACTGT TCCACCTACT 
CCTACCGGGA AGAGTAAAAT CAGCGTTCGC CCTGCAAAAC CTGCTCTGAA AAGTGCTAAT 
AAAGAACATA AGAAAACAGT GGGACATCAG TTCCGGAACT CTCTGCATCT GCTGATGGAA 
ACCTTGAATG CCACAACTCC TCACTATGTG CGCTGCATTA AGCCCAATGA CTATAAGGAG 
TCTTTCAGCT TCGATCCAAA GAGAGCTGTG CAGCAGCTGA GGGCATGTGG CGTGCTGGAG 
ACCATCCGGA TTAGTGCTGC CGGCTATCCA TCCAGGTGGA CCTACCATGA CTTCTTCAAC 
AGGTACCGAG TCCTAATGAA AAAGAAAGAT ATTTCTCTGG GAGACAAGAA GGTGATCTGT 
AAGAATGTCT TAGAGAACCT TATCAAGGAC CCTGACAAGT TCCAGTTTGG ACGAACAAAG 
ATCTTTTTCC GTGCTGGCCA AGTGGCCTAC TTAGAAAAAC TGCGTGCAGA CAAGTTCCGT 
GCCGCTACCA TTATGATTCA GAAGACTGTG CGGGGCTGGC TTCAGAGGGT GAAATACAGA 
AGGATGAAGA AGGCCGCGGT TACCATCCAG AGATATACAC GTGGGCACCT TGCCCGAAGG 
TTGGCTCTGC ACCTCCGAAT GACGAAGGCT GCCATCACCT TCCAGAAGCA ATTCCGAATG 
ATTCGAGTGT ATCGCGGCTT CCAGAAGACT CGCAAGGCAG CCGTCACTAT CCAGTCCTAC 
ACTCGGGGAA TGTTTGTCAG GAGAGCTTAT CGGCAGCTTC TGGAGCAGCA TAAAGCCGCC 
ATCATCCAGA AACACTGGCG CGGATGGAGG GCACGCAAAC TGTTTAGCAA ATTCCGCAGT 
GCCGCTGTCG TTATTCAGTG TTACTTCCGC CGCATGAAAG CCCGACGGGA GCTCAAACAG 
TTGAAGATCG AAGCCCGAAC AGCAGAGCAT TTTAAGAAGC TCAGTGTGGG CATGGAGAAC 
AAGGTGGTTC AGCTGCAGAG AAAGCTTGAT GAGCAGAACA AAGAGCAGAA AGACCTGAAG 
GAGCAACTGG TATCTGCTAC TTCAACTCAT GCTGTCGAGG TTGAGAAACT TCAGAAGGAA 
CTCGAGAAGC AGAGACAGGC TCAGCAGGAT GAGAACCAAC TCACCAGCTT GCAGCAGGAA 
CTGGAGGCTC TGAGAGAGGA ACTGGCCAAA GCGTATAGTG AGAAGAAGGT TGTAGAAGAC 
ACTCTTATGA ATGAGAAAGA GCAACTGCTG CAGCGCGTCT CAGAGCTGGA AGAGGAGAAT 
ACTAACTTGA AAGAAGAAAA AGAAGAACTC AGTAATAAAA TCATTCTACG ATCTGAAGAT 
GAATTTACCC AGAGTACAAT TAAGGAGAAC CTGGAGATCA AGAAAGATCT TGAGGAGGAA 
AGGTCCCGGC ACCAGAATTT GGTAAAGGAA TATACAAGGT TGGAGCAGAG ATATGACAAT 
CTGAAGGAGG AGGTGAACAT TATTAAGCAA CAAATGCCGG GGCACAGGAG GAATCCGTCC 
AACCAGAGCA GCTTGGAATC TGACTCTAAT TACCCTTCCA TATCTACCTC TGAGATTGGG 
GACACAGAGG ATACCATTAA GGAGGTGGAG GAGGCCGGGC TGGAAAAGGC TGCCATGGAC 
ATGTCTCTGT TCCTGAAGCT GCAGAAGAGA GTGAGGGAGC TTGAGCTGGA GAGGAAGAAG 
CTGCAGAGCC AACTGGACAA AAAAGAGCAG CAAGAAAATA AAAAATCCCA GGTTATAGAC 
ACGACCAATG AGGTGATTGC TGACCGCGAC ACTGATATCG CCTACAACAA TCTGAAGCGG 
CAAGAACTTG AGTCTGAAAA CAAGAAGCTG AAAAATGACT TGAATGAGAT GAGGAAGACG 
ATAGCAGATG GAGCAGTAGA TGGTTCGAGT GAAGAGGCCA GAATTGCTAT GTTACTCAAC 
CAACTGAAGT CGGCCCACGA GGAGCTGGAT GTGCGGAAGG AAGAAGTTCT TATGCTCAGA 
ACCCAGATTG TAAAGGCAGC TCAGCAAAAA GAATCAGAGA GGATTATGAG TTCAGAAGCC 
AGTTGGCCAA ACAGCAACAA GGAAATGGAT GAAGACGATG CCATTGAAGC CTATCATGGA 
GTCTGTGAGA CAAACAGGCT TCTGGAGGCT CAGCTTCAGG CACAAAGCAG GCAGCACGAT 
GAGGAGGTGG AGATACTGAC CTCTCACATT GACTCCCTAA AGGAAGAGTT GGAGAAGCAG 
CAGCAGGTTC TACTCCAAGC TCTTCAGCTC TCCCCAGAGG CTCAGGTGGA GTTTGGGCTT 
CAGCATGAAA TTACCCGCCT CACTAATGAG AACCTGGACC TTAAAGAATT ATTGGAGAAA 
TCAGAAAAGA ATGAGAAAAA ATTGAAGAAA CAGCTAAAAA CGTACATGAA GAAAGGACAG 
GATTCTGAAG ATGTAGTGCA GACGGAGAGG AAACCTGAGC TCACCCGTCA AGTGACAGTG 
CAGCGAATTG AGAAGGATTT CCAAGGAATG CTGGAGTATT ACAAGGAGGA AGAACCGCTG 
CTCGTGAGAA ATCTCATTAT AGACGTGAAG CCTGAGCAGA TGTCTTCCAT GGTTCCTTGT 
CTGCCTGCGT ATATCCTCTT CATGTGCATC AGGCACGCCG ACTACATCAA CGATGATCAG 
AAGGTCCACT CCCTGCTCAC CTCCACCATC AACGGGATAA AGAAAGTTCT AAAGAAGCAT 
CACAGTGAAT TCCAGATTAC ATCTTTCTGG CTGTCCAACT CCAGCCGGCT GCTCCATTGC 
CTGAAACAGT ATAGTGGGGA CGAGGGCTTC ATGACCAGCA ATAGCCCCAA GCAGAACGAG 
CATTGCCTGA GGAACTTTGA TCTGACAGAA TACCGCCAGG TCCTCAGCGA CTTGTCTATC 
CAGATCTACC AGCAGATGAT AAAGATTGCG GAAACGCATA TGCAGCCAAT GATAGTGTCT 
GCCATGTTGG AAACAGAAAG CATCCAAGGC CTCTCAGGAG TGAAGCAAAC TGGGTACAGG 
AAGAGGACCT CCAGCATGGC AGATGGGGAT AATTCCTACA GCCTAGAAGC TGTCATTCGC 
CAGCTGAACA CGTTTAACAG CATCATGTGT GACCATGGAC TGGATCCCGA AATCATTCAG 
CAAGTCTTTA AGCAGCTCTT CTACATGATA AATGCTGTAA CGCTGAATAA TCTGCTGCTG 
AGGAAGGACG TTTGCTCATG GAGCACCGGC ATGCAGCTAA GGTATAATAT CAGTCAGTTA 
GAAGAATGGC TGCGAGGGAA GAATTTGCAT CCGAGTGGCG CTGCTCAGAC TATGGAGCCG 
CTGATCCAAG CAGCACAGTT ACTGCAACTC AAAAAGAAGA CAGAGGAGGA TGCAGAGGCC 
ATATGCTCTT TGTGCACAGC CCTGACGACC CAGCAGATTG TGAAGATACT GAATCTGTAC 
ACACCCGTGA ATGAGTTTGA AGAGCGAGTA ACCGTGTCCT TTATCAGAAT CATTCAGGCT 
CAGCTGCAAG AGCGGAACGA TCCTCCCCAG CTGCTAATGG ACTCTAAGTA CATGTTCCCA 
GTCCTGTTCC CATTCAGCCC CTCGGCCATC ACTTTGGACA CCATTCAGAT CCCTCCTTCC 
CTCAAACTGG ATTTCCTCAT GAAGGTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031750784.1
CDS	789..6218
Translation	MSVYELYSQYTRVWIPDPEDVWRSAEIIKDYKEGDKSLQLKLEDESVIQHRVDPNNLPHLRNPDILVGENDLTALSYLHEPAVLHNLKVRFLESNHIYTYCGIVLVAINPYQQLPVYGEDVIYAYSGQNMGDMDPHIFAVAEEAYKQMARDEKNQSIIVSGESGAGKTVSAKYAMRYFATVGGSASEANVEEKVLASSPIMEAIGNAKTTRNDNSSRFGKYIQIGFDKRYHIIGANMRTYLLEKSRVVFQADMERNYHIFYQLCASASLPEFSELSLAEAEEFFYTNQGGDPVIDGVDDAEDFEKTRQAFTLLGVKETHQMGIFKIVASILHLGNVAIQTEREGESCNLSKGDKHLTHFCSLLGLEQDQMQHWLCHRKLVTTSETYVKTMSAEQAVNARNALAKHIYAQLFNWIVQHVNKALHTTIKQHSFIGVLDIYGFETFEINSFEQFCINYANEKLQQQFNSHVFKLEQEEYMKEQIPWTLIDFYDNQPCIDLIEAKLGILDLLDEECKVPKGTDQNWAQKLYDRHGSSQHFKKPRMSNVAFIVVHFADQVEYQCDGFLEKNRDTVYEEQINILKASKYPLVADLFQNEKDTVPPTPTGKSKISVRPAKPALKSANKEHKKTVGHQFRNSLHLLMETLNATTPHYVRCIKPNDYKESFSFDPKRAVQQLRACGVLETIRISAAGYPSRWTYHDFFNRYRVLMKKKDISLGDKKVICKNVLENLIKDPDKFQFGRTKIFFRAGQVAYLEKLRADKFRAATIMIQKTVRGWLQRVKYRRMKKAAVTIQRYTRGHLARRLALHLRMTKAAITFQKQFRMIRVYRGFQKTRKAAVTIQSYTRGMFVRRAYRQLLEQHKAAIIQKHWRGWRARKLFSKFRSAAVVIQCYFRRMKARRELKQLKIEARTAEHFKKLSVGMENKVVQLQRKLDEQNKEQKDLKEQLVSATSTHAVEVEKLQKELEKQRQAQQDENQLTSLQQELEALREELAKAYSEKKVVEDTLMNEKEQLLQRVSELEEENTNLKEEKEELSNKIILRSEDEFTQSTIKENLEIKKDLEEERSRHQNLVKEYTRLEQRYDNLKEEVNIIKQQMPGHRRNPSNQSSLESDSNYPSISTSEIGDTEDTIKEVEEAGLEKAAMDMSLFLKLQKRVRELELERKKLQSQLDKKEQQENKKSQVIDTTNEVIADRDTDIAYNNLKRQELESENKKLKNDLNEMRKTIADGAVDGSSEEARIAMLLNQLKSAHEELDVRKEEVLMLRTQIVKAAQQKESERIMSSEASWPNSNKEMDEDDAIEAYHGVCETNRLLEAQLQAQSRQHDEEVEILTSHIDSLKEELEKQQQVLLQALQLSPEAQVEFGLQHEITRLTNENLDLKELLEKSEKNEKKLKKQLKTYMKKGQDSEDVVQTERKPELTRQVTVQRIEKDFQGMLEYYKEEEPLLVRNLIIDVKPEQMSSMVPCLPAYILFMCIRHADYINDDQKVHSLLTSTINGIKKVLKKHHSEFQITSFWLSNSSRLLHCLKQYSGDEGFMTSNSPKQNEHCLRNFDLTEYRQVLSDLSIQIYQQMIKIAETHMQPMIVSAMLETESIQGLSGVKQTGYRKRTSSMADGDNSYSLEAVIRQLNTFNSIMCDHGLDPEIIQQVFKQLFYMINAVTLNNLLLRKDVCSWSTGMQLRYNISQLEEWLRGKNLHPSGAAQTMEPLIQAAQLLQLKKKTEEDAEAICSLCTALTTQQIVKILNLYTPVNEFEERVTVSFIRIIQAQLQERNDPPQLLMDSKYMFPVLFPFSPSAITLDTIQIPPSLKLDFLMKV

Target ORF information:

RefSeq Version	XM_031894924.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis myosin VB (myo5b), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031894924.1

ORF Insert Sequence:

ATGTCAGTGT ATGAGCTTTA CTCCCAGTAC ACCCGGGTGT GGATACCCGA TCCGGAGGAT 
GTCTGGAGAT CAGCAGAAAT TATCAAGGAC TATAAAGAAG GGGATAAAAG TCTGCAGTTA 
AAACTAGAAG ATGAGTCTGT CATACAGCAC CGTGTGGATC CAAACAACTT ACCGCACCTC 
CGCAACCCAG ATATCCTAGT AGGAGAGAAT GACCTGACGG CTTTGAGCTA TCTCCATGAA 
CCAGCTGTGC TCCATAACCT AAAAGTCCGC TTCCTGGAAT CAAATCACAT TTATACTTAT 
TGCGGAATTG TACTTGTTGC CATAAACCCA TACCAGCAGT TACCAGTCTA TGGAGAAGAT 
GTCATTTACG CATACAGCGG CCAAAATATG GGAGACATGG ACCCCCACAT ATTTGCAGTG 
GCTGAAGAGG CCTATAAGCA AATGGCCAGG GATGAAAAGA ACCAGTCTAT CATCGTAAGT 
GGAGAGTCCG GAGCAGGGAA AACTGTCTCT GCCAAATATG CCATGCGCTA CTTTGCCACA 
GTGGGCGGCT CAGCAAGTGA AGCTAATGTG GAAGAGAAAG TATTGGCTTC TAGTCCCATC 
ATGGAGGCTA TTGGGAATGC CAAAACAACC CGGAATGACA ACAGCAGCCG ATTTGGAAAG 
TACATTCAGA TTGGGTTTGA CAAACGATAT CATATTATCG GTGCCAACAT GAGGACTTAC 
CTTCTTGAGA AATCACGTGT TGTTTTCCAG GCTGATATGG AAAGGAACTA CCATATTTTC 
TACCAGCTCT GTGCCTCTGC CAGCCTGCCG GAGTTCTCCG AGCTGTCATT AGCTGAAGCT 
GAGGAGTTCT TTTACACCAA CCAGGGAGGC GACCCCGTTA TTGATGGAGT GGATGATGCT 
GAAGACTTTG AGAAGACGAG ACAGGCTTTC ACACTTTTGG GAGTGAAGGA GACTCATCAA 
ATGGGCATCT TTAAGATTGT GGCTTCCATT CTGCACCTTG GAAATGTAGC CATCCAGACG 
GAGCGGGAAG GGGAATCGTG TAACCTGTCG AAAGGGGATA AACACCTGAC TCATTTCTGC 
AGTTTGCTTG GGCTGGAGCA GGACCAGATG CAACACTGGC TGTGCCACCG GAAACTTGTC 
ACCACCTCCG AAACGTACGT CAAGACCATG TCTGCGGAGC AGGCTGTAAA TGCCAGGAAC 
GCCTTGGCCA AGCACATCTA CGCCCAGCTC TTCAACTGGA TAGTGCAGCA CGTGAACAAG 
GCCCTGCACA CCACCATCAA ACAGCACTCC TTTATTGGGG TCCTCGACAT ATACGGGTTT 
GAAACCTTTG AAATTAACAG CTTTGAGCAA TTTTGCATCA ATTACGCCAA TGAGAAGCTG 
CAACAGCAAT TTAATTCGCA CGTCTTTAAA CTGGAACAAG AAGAATACAT GAAAGAGCAG 
ATTCCATGGA CCCTGATAGA CTTCTACGAC AACCAACCTT GTATTGACCT GATTGAGGCA 
AAATTAGGCA TTTTGGACCT TCTAGATGAA GAGTGCAAGG TCCCCAAAGG AACTGATCAG 
AACTGGGCAC AGAAGTTATA TGACCGGCAT GGAAGCTCCC AACACTTCAA GAAACCACGA 
ATGTCTAATG TGGCATTTAT TGTGGTGCAT TTTGCTGATC AGGTGGAGTA TCAATGTGAC 
GGATTCCTGG AGAAGAACAG GGACACTGTG TATGAGGAAC AGATTAACAT ACTAAAGGCC 
TCAAAGTATC CGCTGGTTGC TGACCTGTTT CAAAATGAAA AAGACACTGT TCCACCTACT 
CCTACCGGGA AGAGTAAAAT CAGCGTTCGC CCTGCAAAAC CTGCTCTGAA AAGTGCTAAT 
AAAGAACATA AGAAAACAGT GGGACATCAG TTCCGGAACT CTCTGCATCT GCTGATGGAA 
ACCTTGAATG CCACAACTCC TCACTATGTG CGCTGCATTA AGCCCAATGA CTATAAGGAG 
TCTTTCAGCT TCGATCCAAA GAGAGCTGTG CAGCAGCTGA GGGCATGTGG CGTGCTGGAG 
ACCATCCGGA TTAGTGCTGC CGGCTATCCA TCCAGGTGGA CCTACCATGA CTTCTTCAAC 
AGGTACCGAG TCCTAATGAA AAAGAAAGAT ATTTCTCTGG GAGACAAGAA GGTGATCTGT 
AAGAATGTCT TAGAGAACCT TATCAAGGAC CCTGACAAGT TCCAGTTTGG ACGAACAAAG 
ATCTTTTTCC GTGCTGGCCA AGTGGCCTAC TTAGAAAAAC TGCGTGCAGA CAAGTTCCGT 
GCCGCTACCA TTATGATTCA GAAGACTGTG CGGGGCTGGC TTCAGAGGGT GAAATACAGA 
AGGATGAAGA AGGCCGCGGT TACCATCCAG AGATATACAC GTGGGCACCT TGCCCGAAGG 
TTGGCTCTGC ACCTCCGAAT GACGAAGGCT GCCATCACCT TCCAGAAGCA ATTCCGAATG 
ATTCGAGTGT ATCGCGGCTT CCAGAAGACT CGCAAGGCAG CCGTCACTAT CCAGTCCTAC 
ACTCGGGGAA TGTTTGTCAG GAGAGCTTAT CGGCAGCTTC TGGAGCAGCA TAAAGCCGCC 
ATCATCCAGA AACACTGGCG CGGATGGAGG GCACGCAAAC TGTTTAGCAA ATTCCGCAGT 
GCCGCTGTCG TTATTCAGTG TTACTTCCGC CGCATGAAAG CCCGACGGGA GCTCAAACAG 
TTGAAGATCG AAGCCCGAAC AGCAGAGCAT TTTAAGAAGC TCAGTGTGGG CATGGAGAAC 
AAGGTGGTTC AGCTGCAGAG AAAGCTTGAT GAGCAGAACA AAGAGCAGAA AGACCTGAAG 
GAGCAACTGG TATCTGCTAC TTCAACTCAT GCTGTCGAGG TTGAGAAACT TCAGAAGGAA 
CTCGAGAAGC AGAGACAGGC TCAGCAGGAT GAGAACCAAC TCACCAGCTT GCAGCAGGAA 
CTGGAGGCTC TGAGAGAGGA ACTGGCCAAA GCGTATAGTG AGAAGAAGGT TGTAGAAGAC 
ACTCTTATGA ATGAGAAAGA GCAACTGCTG CAGCGCGTCT CAGAGCTGGA AGAGGAGAAT 
ACTAACTTGA AAGAAGAAAA AGAAGAACTC AGTAATAAAA TCATTCTACG ATCTGAAGAT 
GAATTTACCC AGAGTACAAT TAAGGAGAAC CTGGAGATCA AGAAAGATCT TGAGGAGGAA 
AGGTCCCGGC ACCAGAATTT GGTAAAGGAA TATACAAGGT TGGAGCAGAG ATATGACAAT 
CTGAAGGAGG AGGTGAACAT TATTAAGCAA CAAATGCCGG GGCACAGGAG GAATCCGTCC 
AACCAGAGCA GCTTGGAATC TGACTCTAAT TACCCTTCCA TATCTACCTC TGAGATTGGG 
GACACAGAGG ATACCATTAA GGAGGTGGAG GAGGCCGGGC TGGAAAAGGC TGCCATGGAC 
ATGTCTCTGT TCCTGAAGCT GCAGAAGAGA GTGAGGGAGC TTGAGCTGGA GAGGAAGAAG 
CTGCAGAGCC AACTGGACAA AAAAGAGCAG CAAGAAAATA AAAAATCCCA GGTTATAGAC 
ACGACCAATG AGGTGATTGC TGACCGCGAC ACTGATATCG CCTACAACAA TCTGAAGCGG 
CAAGAACTTG AGTCTGAAAA CAAGAAGCTG AAAAATGACT TGAATGAGAT GAGGAAGACG 
ATAGCAGATG GAGCAGTAGA TGGTTCGAGT GAAGAGGCCA GAATTGCTAT GTTACTCAAC 
CAACTGAAGT CGGCCCACGA GGAGCTGGAT GTGCGGAAGG AAGAAGTTCT TATGCTCAGA 
ACCCAGATTG TAAAGGCAGC TCAGCAAAAA GAATCAGAGA GGATTATGAG TTCAGAAGCC 
AGTTGGCCAA ACAGCAACAA GGAAATGGAT GAAGACGATG CCATTGAAGC CTATCATGGA 
GTCTGTGAGA CAAACAGGCT TCTGGAGGCT CAGCTTCAGG CACAAAGCAG GCAGCACGAT 
GAGGAGGTGG AGATACTGAC CTCTCACATT GACTCCCTAA AGGAAGAGTT GGAGAAGCAG 
CAGCAGGTTC TACTCCAAGC TCTTCAGCTC TCCCCAGAGG CTCAGGTGGA GTTTGGGCTT 
CAGCATGAAA TTACCCGCCT CACTAATGAG AACCTGGACC TTAAAGAATT ATTGGAGAAA 
TCAGAAAAGA ATGAGAAAAA ATTGAAGAAA CAGCTAAAAA CGTACATGAA GAAAGGACAG 
GATTCTGAAG ATGTAGTGCA GACGGAGAGG AAACCTGAGC TCACCCGTCA AGTGACAGTG 
CAGCGAATTG AGAAGGATTT CCAAGGAATG CTGGAGTATT ACAAGGAGGA AGAACCGCTG 
CTCGTGAGAA ATCTCATTAT AGACGTGAAG CCTGAGCAGA TGTCTTCCAT GGTTCCTTGT 
CTGCCTGCGT ATATCCTCTT CATGTGCATC AGGCACGCCG ACTACATCAA CGATGATCAG 
AAGGTCCACT CCCTGCTCAC CTCCACCATC AACGGGATAA AGAAAGTTCT AAAGAAGCAT 
CACAGTGAAT TCCAGATTAC ATCTTTCTGG CTGTCCAACT CCAGCCGGCT GCTCCATTGC 
CTGAAACAGT ATAGTGGGGA CGAGGGCTTC ATGACCAGCA ATAGCCCCAA GCAGAACGAG 
CATTGCCTGA GGAACTTTGA TCTGACAGAA TACCGCCAGG TCCTCAGCGA CTTGTCTATC 
CAGATCTACC AGCAGATGAT AAAGATTGCG GAAACGCATA TGCAGCCAAT GATAGTGTCT 
GCCATGTTGG AAACAGAAAG CATCCAAGGC CTCTCAGGAG TGAAGCAAAC TGGGTACAGG 
AAGAGGACCT CCAGCATGGC AGATGGGGAT AATTCCTACA GCCTAGAAGC TGTCATTCGC 
CAGCTGAACA CGTTTAACAG CATCATGTGT GACCATGGAC TGGATCCCGA AATCATTCAG 
CAAGTCTTTA AGCAGCTCTT CTACATGATA AATGCTGTAA CGCTGAATAA TCTGCTGCTG 
AGGAAGGACG TTTGCTCATG GAGCACCGGC ATGCAGCTAA GGTATAATAT CAGTCAGTTA 
GAAGAATGGC TGCGAGGGAA GAATTTGCAT CCGAGTGGCG CTGCTCAGAC TATGGAGCCG 
CTGATCCAAG CAGCACAGTT ACTGCAACTC AAAAAGAAGA CAGAGGAGGA TGCAGAGGCC 
ATATGCTCTT TGTGCACAGC CCTGACGACC CAGCAGATTG TGAAGATACT GAATCTGTAC 
ACACCCGTGA ATGAGTTTGA AGAGCGAGTA ACCGTGTCCT TTATCAGAAT CATTCAGGCT 
CAGCTGCAAG AGCGGAACGA TCCTCCCCAG CTGCTAATGG ACTCTAAGTA CATGTTCCCA 
GTCCTGTTCC CATTCAGCCC CTCGGCCATC ACTTTGGACA CCATTCAGAT CCCTCCTTCC 
CTCAAACTGG ATTTCCTCAT GAAGGTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

myo5b ( XM_031894925.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_031750785.1 Xenopus tropicalis myosin VB (myo5b), transcript variant X2, mRNA.

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CloneID	OXa49263
Clone ID Related Accession (Same CDS sequence)	XM_031894925.1
Accession Version	XM_031894925.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5508bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1576512000000
Organism	Xenopus tropicalis(tropical clawed frog)
Product	unconventional myosin-Vb isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCAGTGT ATGAGCTTTA CTCCCAGTAC ACCCGGGTGT GGATACCCGA TCCGGAGGAT 
GTCTGGAGAT CAGCAGAAAT TATCAAGGAC TATAAAGAAG GGGATAAAAG TCTGCAGTTA 
AAACTAGAAG ATGAGTCTGT CATACAGCAC CGTGTGGATC CAAACAACTT ACCGCACCTC 
CGCAACCCAG ATATCCTAGT AGGAGAGAAT GACCTGACGG CTTTGAGCTA TCTCCATGAA 
CCAGCTGTGC TCCATAACCT AAAAGTCCGC TTCCTGGAAT CAAATCACAT TTATACTTAT 
TGCGGAATTG TACTTGTTGC CATAAACCCA TACCAGCAGT TACCAGTCTA TGGAGAAGAT 
GTCATTTACG CATACAGCGG CCAAAATATG GGAGACATGG ACCCCCACAT ATTTGCAGTG 
GCTGAAGAGG CCTATAAGCA AATGGCCAGG GATGAAAAGA ACCAGTCTAT CATCGTAAGT 
GGAGAGTCCG GAGCAGGGAA AACTGTCTCT GCCAAATATG CCATGCGCTA CTTTGCCACA 
GTGGGCGGCT CAGCAAGTGA AGCTAATGTG GAAGAGAAAG TATTGGCTTC TAGTCCCATC 
ATGGAGGCTA TTGGGAATGC CAAAACAACC CGGAATGACA ACAGCAGCCG ATTTGGAAAG 
TACATTCAGA TTGGGTTTGA CAAACGATAT CATATTATCG GTGCCAACAT GAGGACTTAC 
CTTCTTGAGA AATCACGTGT TGTTTTCCAG GCTGATATGG AAAGGAACTA CCATATTTTC 
TACCAGCTCT GTGCCTCTGC CAGCCTGCCG GAGTTCTCCG AGCTGTCATT AGCTGAAGCT 
GAGGAGTTCT TTTACACCAA CCAGGGAGGC GACCCCGTTA TTGATGGAGT GGATGATGCT 
GAAGACTTTG AGAAGACGAG ACAGGCTTTC ACACTTTTGG GAGTGAAGGA GACTCATCAA 
ATGGGCATCT TTAAGATTGT GGCTTCCATT CTGCACCTTG GAAATGTAGC CATCCAGACG 
GAGCGGGAAG GGGAATCGTG TAACCTGTCG AAAGGGGATA AACACCTGAC TCATTTCTGC 
AGTTTGCTTG GGCTGGAGCA GGACCAGATG CAACACTGGC TGTGCCACCG GAAACTTGTC 
ACCACCTCCG AAACGTACGT CAAGACCATG TCTGCGGAGC AGGCTGTAAA TGCCAGGAAC 
GCCTTGGCCA AGCACATCTA CGCCCAGCTC TTCAACTGGA TAGTGCAGCA CGTGAACAAG 
GCCCTGCACA CCACCATCAA ACAGCACTCC TTTATTGGGG TCCTCGACAT ATACGGGTTT 
GAAACCTTTG AAATTAACAG CTTTGAGCAA TTTTGCATCA ATTACGCCAA TGAGAAGCTG 
CAACAGCAAT TTAATTCGCA CGTCTTTAAA CTGGAACAAG AAGAATACAT GAAAGAGCAG 
ATTCCATGGA CCCTGATAGA CTTCTACGAC AACCAACCTT GTATTGACCT GATTGAGGCA 
AAATTAGGCA TTTTGGACCT TCTAGATGAA GAGTGCAAGG TCCCCAAAGG AACTGATCAG 
AACTGGGCAC AGAAGTTATA TGACCGGCAT GGAAGCTCCC AACACTTCAA GAAACCACGA 
ATGTCTAATG TGGCATTTAT TGTGGTGCAT TTTGCTGATC AGGTGGAGTA TCAATGTGAC 
GGATTCCTGG AGAAGAACAG GGACACTGTG TATGAGGAAC AGATTAACAT ACTAAAGGCC 
TCAAAGTATC CGCTGGTTGC TGACCTGTTT CAAAATGAAA AAGACACTGT TCCACCTACT 
CCTACCGGGA AGAGTAAAAT CAGCGTTCGC CCTGCAAAAC CTGCTCTGAA AAGTGCTAAT 
AAAGAACATA AGAAAACAGT GGGACATCAG TTCCGGAACT CTCTGCATCT GCTGATGGAA 
ACCTTGAATG CCACAACTCC TCACTATGTG CGCTGCATTA AGCCCAATGA CTATAAGGAG 
TCTTTCAGCT TCGATCCAAA GAGAGCTGTG CAGCAGCTGA GGGCATGTGG CGTGCTGGAG 
ACCATCCGGA TTAGTGCTGC CGGCTATCCA TCCAGGTGGA CCTACCATGA CTTCTTCAAC 
AGGTACCGAG TCCTAATGAA AAAGAAAGAT ATTTCTCTGG GAGACAAGAA GGTGATCTGT 
AAGAATGTCT TAGAGAACCT TATCAAGGAC CCTGACAAGT TCCAGTTTGG ACGAACAAAG 
ATCTTTTTCC GTGCTGGCCA AGTGGCCTAC TTAGAAAAAC TGCGTGCAGA CAAGTTCCGT 
GCCGCTACCA TTATGATTCA GAAGACTGTG CGGGGCTGGC TTCAGAGGGT GAAATACAGA 
AGGATGAAGA AGGCCGCGGT TACCATCCAG AGATATACAC GTGGGCACCT TGCCCGAAGG 
TTGGCTCTGC ACCTCCGAAT GACGAAGGCT GCCATCACCT TCCAGAAGCA ATTCCGAATG 
ATTCGAGTGT ATCGCGGCTT CCAGAAGACT CGCAAGGCAG CCGTCACTAT CCAGTCCTAC 
ACTCGGGGAA TGTTTGTCAG GAGAGCTTAT CGGCAGCTTC TGGAGCAGCA TAAAGCCGCC 
ATCATCCAGA AACACTGGCG CGGATGGAGG GCACGCAAAC TGTTTAGCAA ATTCCGCAGT 
GCCGCTGTCG TTATTCAGTG TTACTTCCGC CGCATGAAAG CCCGACGGGA GCTCAAACAG 
TTGAAGATCG AAGCCCGAAC AGCAGAGCAT TTTAAGAAGC TCAGTGTGGG CATGGAGAAC 
AAGGTGGTTC AGCTGCAGAG AAAGCTTGAT GAGCAGAACA AAGAGCAGAA AGACCTGAAG 
GAGCAACTGG TATCTGCTAC TTCAACTCAT GCTGTCGAGG TTGAGAAACT TCAGAAGGAA 
CTCGAGAAGC AGAGACAGGC TCAGCAGGAT GAGAACCAAC TCACCAGCTT GCAGCAGGAA 
CTGGAGGCTC TGAGAGAGGA ACTGGCCAAA GCGTATAGTG AGAAGAAGGT TGTAGAAGAC 
ACTCTTATGA ATGAGAAAGA GCAACTGCTG CAGCGCGTCT CAGAGCTGGA AGAGGAGAAT 
ACTAACTTGA AAGAAGAAAA AGAAGAACTC AGTAATAAAA TCATTCTACG ATCTGAAGAT 
GAATTTACCC AGAGTACAAT TAAGGAGAAC CTGGAGATCA AGAAAGATCT TGAGGAGGAA 
AGGTCCCGGC ACCAGAATTT GGTAAAGGAA TATACAAGGT TGGAGCAGAG ATATGACAAT 
CTGAAGGAGG AGGTGAACAT TATTAAGCAA CAAATGCCGG GGCACAGGAG GAATCCGTCC 
AACCAGAGCA GCTTGGAATC TGACTCTAAT TACCCTTCCA TATCTACCTC TGAGATTGGG 
GACACAGAGG ATACCATTAA GGAGGTGGAG GAGGCCGGGC TGGAAAAGGC TGCCATGGAC 
ATGTCTCTGT TCCTGAAGCT GCAGAAGAGA GTGAGGGAGC TTGAGCTGGA GAGGAAGAAG 
CTGCAGAGCC AACTGGACAA AAAAGAGCAG CAAGAAAATA AAAAATCCCA GGTTATAGAC 
ACGACCAATG AGGTGATTGC TGACCGCGAC ACTGATATCG CCTACAACAA TCTGAAGCGG 
CAAGAACTTG AGTCTGAAAA CAAGAAGCTG AAAAATGACT TGAATGAGAT GAGGAAGACG 
ATAGCAGATG GAGCAGTAGA TGGTTCGAGT GAAGAGGCCA GAATTGCTAT GTTACTCAAC 
CAACTGAAGT CGGCCCACGA GGAGCTGGAT GTGCGGAAGG AAGAAGTTCT TATGCTCAGA 
ACCCAGATTG TAAAGGCAGC TCAGCAAAAA GAATCAGAGA GGATTATGAG TTCAGAAGCC 
AGTTGGCCAA ACAGCAACAA GGAAATGGAT GAAGACGATG CCATTGAAGC CTATCATGGA 
GTCTGTGAGA CAAACAGAAA GACTCAGGAC TGGGGGTATC TAAATGAAGA TGGGGAGCTT 
GGCCTGGCCT ATCAAGGCTT AAAGCAAGTT GCCAGGCTTC TGGAGGCTCA GCTTCAGGCA 
CAAAGCAGGC AGCACGATGA GGAGGTGGAG ATACTGACCT CTCACATTGA CTCCCTAAAG 
GAAGAGTTGG AGAAGCAGCA GCAGGTTCTA CTCCAAGCTC TTCAGCTCTC CCCAGAGGCT 
CAGGTGGAGT TTGGGCTTCA GCATGAAATT ACCCGCCTCA CTAATGAGAA CCTGGACCTT 
AAAGAATTAT TGGAGAAATC AGAAAAGAAT GAGAAAAAAT TGAAGAAACA GCTAAAAACG 
TACATGAAGA AAGGACAGGA TTCTGAAGAT GTAGTGCAGA CGGAGAGGAA ACCTGAGCTC 
ACCCGTCAAG TGACAGTGCA GCGAATTGAG AAGGATTTCC AAGGAATGCT GGAGTATTAC 
AAGGAGGAAG AACCGCTGCT CGTGAGAAAT CTCATTATAG ACGTGAAGCC TGAGCAGATG 
TCTTCCATGG TTCCTTGTCT GCCTGCGTAT ATCCTCTTCA TGTGCATCAG GCACGCCGAC 
TACATCAACG ATGATCAGAA GGTCCACTCC CTGCTCACCT CCACCATCAA CGGGATAAAG 
AAAGTTCTAA AGAAGCATCA CAGTGAATTC CAGATTACAT CTTTCTGGCT GTCCAACTCC 
AGCCGGCTGC TCCATTGCCT GAAACAGTAT AGTGGGGACG AGGGCTTCAT GACCAGCAAT 
AGCCCCAAGC AGAACGAGCA TTGCCTGAGG AACTTTGATC TGACAGAATA CCGCCAGGTC 
CTCAGCGACT TGTCTATCCA GATCTACCAG CAGATGATAA AGATTGCGGA AACGCATATG 
CAGCCAATGA TAGTGTCTGC CATGTTGGAA ACAGAAAGCA TCCAAGGCCT CTCAGGAGTG 
AAGCAAACTG GGTACAGGAA GAGGACCTCC AGCATGGCAG ATGGGGATAA TTCCTACAGC 
CTAGAAGCTG TCATTCGCCA GCTGAACACG TTTAACAGCA TCATGTGTGA CCATGGACTG 
GATCCCGAAA TCATTCAGCA AGTCTTTAAG CAGCTCTTCT ACATGATAAA TGCTGTAACG 
CTGAATAATC TGCTGCTGAG GAAGGACGTT TGCTCATGGA GCACCGGCAT GCAGCTAAGG 
TATAATATCA GTCAGTTAGA AGAATGGCTG CGAGGGAAGA ATTTGCATCC GAGTGGCGCT 
GCTCAGACTA TGGAGCCGCT GATCCAAGCA GCACAGTTAC TGCAACTCAA AAAGAAGACA 
GAGGAGGATG CAGAGGCCAT ATGCTCTTTG TGCACAGCCC TGACGACCCA GCAGATTGTG 
AAGATACTGA ATCTGTACAC ACCCGTGAAT GAGTTTGAAG AGCGAGTAAC CGTGTCCTTT 
ATCAGAATCA TTCAGGCTCA GCTGCAAGAG CGGAACGATC CTCCCCAGCT GCTAATGGAC 
TCTAAGTACA TGTTCCCAGT CCTGTTCCCA TTCAGCCCCT CGGCCATCAC TTTGGACACC 
ATTCAGATCC CTCCTTCCCT CAAACTGGAT TTCCTCATGA AGGTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031750785.1
CDS	790..6297
Translation	MSVYELYSQYTRVWIPDPEDVWRSAEIIKDYKEGDKSLQLKLEDESVIQHRVDPNNLPHLRNPDILVGENDLTALSYLHEPAVLHNLKVRFLESNHIYTYCGIVLVAINPYQQLPVYGEDVIYAYSGQNMGDMDPHIFAVAEEAYKQMARDEKNQSIIVSGESGAGKTVSAKYAMRYFATVGGSASEANVEEKVLASSPIMEAIGNAKTTRNDNSSRFGKYIQIGFDKRYHIIGANMRTYLLEKSRVVFQADMERNYHIFYQLCASASLPEFSELSLAEAEEFFYTNQGGDPVIDGVDDAEDFEKTRQAFTLLGVKETHQMGIFKIVASILHLGNVAIQTEREGESCNLSKGDKHLTHFCSLLGLEQDQMQHWLCHRKLVTTSETYVKTMSAEQAVNARNALAKHIYAQLFNWIVQHVNKALHTTIKQHSFIGVLDIYGFETFEINSFEQFCINYANEKLQQQFNSHVFKLEQEEYMKEQIPWTLIDFYDNQPCIDLIEAKLGILDLLDEECKVPKGTDQNWAQKLYDRHGSSQHFKKPRMSNVAFIVVHFADQVEYQCDGFLEKNRDTVYEEQINILKASKYPLVADLFQNEKDTVPPTPTGKSKISVRPAKPALKSANKEHKKTVGHQFRNSLHLLMETLNATTPHYVRCIKPNDYKESFSFDPKRAVQQLRACGVLETIRISAAGYPSRWTYHDFFNRYRVLMKKKDISLGDKKVICKNVLENLIKDPDKFQFGRTKIFFRAGQVAYLEKLRADKFRAATIMIQKTVRGWLQRVKYRRMKKAAVTIQRYTRGHLARRLALHLRMTKAAITFQKQFRMIRVYRGFQKTRKAAVTIQSYTRGMFVRRAYRQLLEQHKAAIIQKHWRGWRARKLFSKFRSAAVVIQCYFRRMKARRELKQLKIEARTAEHFKKLSVGMENKVVQLQRKLDEQNKEQKDLKEQLVSATSTHAVEVEKLQKELEKQRQAQQDENQLTSLQQELEALREELAKAYSEKKVVEDTLMNEKEQLLQRVSELEEENTNLKEEKEELSNKIILRSEDEFTQSTIKENLEIKKDLEEERSRHQNLVKEYTRLEQRYDNLKEEVNIIKQQMPGHRRNPSNQSSLESDSNYPSISTSEIGDTEDTIKEVEEAGLEKAAMDMSLFLKLQKRVRELELERKKLQSQLDKKEQQENKKSQVIDTTNEVIADRDTDIAYNNLKRQELESENKKLKNDLNEMRKTIADGAVDGSSEEARIAMLLNQLKSAHEELDVRKEEVLMLRTQIVKAAQQKESERIMSSEASWPNSNKEMDEDDAIEAYHGVCETNRKTQDWGYLNEDGELGLAYQGLKQVARLLEAQLQAQSRQHDEEVEILTSHIDSLKEELEKQQQVLLQALQLSPEAQVEFGLQHEITRLTNENLDLKELLEKSEKNEKKLKKQLKTYMKKGQDSEDVVQTERKPELTRQVTVQRIEKDFQGMLEYYKEEEPLLVRNLIIDVKPEQMSSMVPCLPAYILFMCIRHADYINDDQKVHSLLTSTINGIKKVLKKHHSEFQITSFWLSNSSRLLHCLKQYSGDEGFMTSNSPKQNEHCLRNFDLTEYRQVLSDLSIQIYQQMIKIAETHMQPMIVSAMLETESIQGLSGVKQTGYRKRTSSMADGDNSYSLEAVIRQLNTFNSIMCDHGLDPEIIQQVFKQLFYMINAVTLNNLLLRKDVCSWSTGMQLRYNISQLEEWLRGKNLHPSGAAQTMEPLIQAAQLLQLKKKTEEDAEAICSLCTALTTQQIVKILNLYTPVNEFEERVTVSFIRIIQAQLQERNDPPQLLMDSKYMFPVLFPFSPSAITLDTIQIPPSLKLDFLMKV

Target ORF information:

RefSeq Version	XM_031894925.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis myosin VB (myo5b), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031894925.1

ORF Insert Sequence:

ATGTCAGTGT ATGAGCTTTA CTCCCAGTAC ACCCGGGTGT GGATACCCGA TCCGGAGGAT 
GTCTGGAGAT CAGCAGAAAT TATCAAGGAC TATAAAGAAG GGGATAAAAG TCTGCAGTTA 
AAACTAGAAG ATGAGTCTGT CATACAGCAC CGTGTGGATC CAAACAACTT ACCGCACCTC 
CGCAACCCAG ATATCCTAGT AGGAGAGAAT GACCTGACGG CTTTGAGCTA TCTCCATGAA 
CCAGCTGTGC TCCATAACCT AAAAGTCCGC TTCCTGGAAT CAAATCACAT TTATACTTAT 
TGCGGAATTG TACTTGTTGC CATAAACCCA TACCAGCAGT TACCAGTCTA TGGAGAAGAT 
GTCATTTACG CATACAGCGG CCAAAATATG GGAGACATGG ACCCCCACAT ATTTGCAGTG 
GCTGAAGAGG CCTATAAGCA AATGGCCAGG GATGAAAAGA ACCAGTCTAT CATCGTAAGT 
GGAGAGTCCG GAGCAGGGAA AACTGTCTCT GCCAAATATG CCATGCGCTA CTTTGCCACA 
GTGGGCGGCT CAGCAAGTGA AGCTAATGTG GAAGAGAAAG TATTGGCTTC TAGTCCCATC 
ATGGAGGCTA TTGGGAATGC CAAAACAACC CGGAATGACA ACAGCAGCCG ATTTGGAAAG 
TACATTCAGA TTGGGTTTGA CAAACGATAT CATATTATCG GTGCCAACAT GAGGACTTAC 
CTTCTTGAGA AATCACGTGT TGTTTTCCAG GCTGATATGG AAAGGAACTA CCATATTTTC 
TACCAGCTCT GTGCCTCTGC CAGCCTGCCG GAGTTCTCCG AGCTGTCATT AGCTGAAGCT 
GAGGAGTTCT TTTACACCAA CCAGGGAGGC GACCCCGTTA TTGATGGAGT GGATGATGCT 
GAAGACTTTG AGAAGACGAG ACAGGCTTTC ACACTTTTGG GAGTGAAGGA GACTCATCAA 
ATGGGCATCT TTAAGATTGT GGCTTCCATT CTGCACCTTG GAAATGTAGC CATCCAGACG 
GAGCGGGAAG GGGAATCGTG TAACCTGTCG AAAGGGGATA AACACCTGAC TCATTTCTGC 
AGTTTGCTTG GGCTGGAGCA GGACCAGATG CAACACTGGC TGTGCCACCG GAAACTTGTC 
ACCACCTCCG AAACGTACGT CAAGACCATG TCTGCGGAGC AGGCTGTAAA TGCCAGGAAC 
GCCTTGGCCA AGCACATCTA CGCCCAGCTC TTCAACTGGA TAGTGCAGCA CGTGAACAAG 
GCCCTGCACA CCACCATCAA ACAGCACTCC TTTATTGGGG TCCTCGACAT ATACGGGTTT 
GAAACCTTTG AAATTAACAG CTTTGAGCAA TTTTGCATCA ATTACGCCAA TGAGAAGCTG 
CAACAGCAAT TTAATTCGCA CGTCTTTAAA CTGGAACAAG AAGAATACAT GAAAGAGCAG 
ATTCCATGGA CCCTGATAGA CTTCTACGAC AACCAACCTT GTATTGACCT GATTGAGGCA 
AAATTAGGCA TTTTGGACCT TCTAGATGAA GAGTGCAAGG TCCCCAAAGG AACTGATCAG 
AACTGGGCAC AGAAGTTATA TGACCGGCAT GGAAGCTCCC AACACTTCAA GAAACCACGA 
ATGTCTAATG TGGCATTTAT TGTGGTGCAT TTTGCTGATC AGGTGGAGTA TCAATGTGAC 
GGATTCCTGG AGAAGAACAG GGACACTGTG TATGAGGAAC AGATTAACAT ACTAAAGGCC 
TCAAAGTATC CGCTGGTTGC TGACCTGTTT CAAAATGAAA AAGACACTGT TCCACCTACT 
CCTACCGGGA AGAGTAAAAT CAGCGTTCGC CCTGCAAAAC CTGCTCTGAA AAGTGCTAAT 
AAAGAACATA AGAAAACAGT GGGACATCAG TTCCGGAACT CTCTGCATCT GCTGATGGAA 
ACCTTGAATG CCACAACTCC TCACTATGTG CGCTGCATTA AGCCCAATGA CTATAAGGAG 
TCTTTCAGCT TCGATCCAAA GAGAGCTGTG CAGCAGCTGA GGGCATGTGG CGTGCTGGAG 
ACCATCCGGA TTAGTGCTGC CGGCTATCCA TCCAGGTGGA CCTACCATGA CTTCTTCAAC 
AGGTACCGAG TCCTAATGAA AAAGAAAGAT ATTTCTCTGG GAGACAAGAA GGTGATCTGT 
AAGAATGTCT TAGAGAACCT TATCAAGGAC CCTGACAAGT TCCAGTTTGG ACGAACAAAG 
ATCTTTTTCC GTGCTGGCCA AGTGGCCTAC TTAGAAAAAC TGCGTGCAGA CAAGTTCCGT 
GCCGCTACCA TTATGATTCA GAAGACTGTG CGGGGCTGGC TTCAGAGGGT GAAATACAGA 
AGGATGAAGA AGGCCGCGGT TACCATCCAG AGATATACAC GTGGGCACCT TGCCCGAAGG 
TTGGCTCTGC ACCTCCGAAT GACGAAGGCT GCCATCACCT TCCAGAAGCA ATTCCGAATG 
ATTCGAGTGT ATCGCGGCTT CCAGAAGACT CGCAAGGCAG CCGTCACTAT CCAGTCCTAC 
ACTCGGGGAA TGTTTGTCAG GAGAGCTTAT CGGCAGCTTC TGGAGCAGCA TAAAGCCGCC 
ATCATCCAGA AACACTGGCG CGGATGGAGG GCACGCAAAC TGTTTAGCAA ATTCCGCAGT 
GCCGCTGTCG TTATTCAGTG TTACTTCCGC CGCATGAAAG CCCGACGGGA GCTCAAACAG 
TTGAAGATCG AAGCCCGAAC AGCAGAGCAT TTTAAGAAGC TCAGTGTGGG CATGGAGAAC 
AAGGTGGTTC AGCTGCAGAG AAAGCTTGAT GAGCAGAACA AAGAGCAGAA AGACCTGAAG 
GAGCAACTGG TATCTGCTAC TTCAACTCAT GCTGTCGAGG TTGAGAAACT TCAGAAGGAA 
CTCGAGAAGC AGAGACAGGC TCAGCAGGAT GAGAACCAAC TCACCAGCTT GCAGCAGGAA 
CTGGAGGCTC TGAGAGAGGA ACTGGCCAAA GCGTATAGTG AGAAGAAGGT TGTAGAAGAC 
ACTCTTATGA ATGAGAAAGA GCAACTGCTG CAGCGCGTCT CAGAGCTGGA AGAGGAGAAT 
ACTAACTTGA AAGAAGAAAA AGAAGAACTC AGTAATAAAA TCATTCTACG ATCTGAAGAT 
GAATTTACCC AGAGTACAAT TAAGGAGAAC CTGGAGATCA AGAAAGATCT TGAGGAGGAA 
AGGTCCCGGC ACCAGAATTT GGTAAAGGAA TATACAAGGT TGGAGCAGAG ATATGACAAT 
CTGAAGGAGG AGGTGAACAT TATTAAGCAA CAAATGCCGG GGCACAGGAG GAATCCGTCC 
AACCAGAGCA GCTTGGAATC TGACTCTAAT TACCCTTCCA TATCTACCTC TGAGATTGGG 
GACACAGAGG ATACCATTAA GGAGGTGGAG GAGGCCGGGC TGGAAAAGGC TGCCATGGAC 
ATGTCTCTGT TCCTGAAGCT GCAGAAGAGA GTGAGGGAGC TTGAGCTGGA GAGGAAGAAG 
CTGCAGAGCC AACTGGACAA AAAAGAGCAG CAAGAAAATA AAAAATCCCA GGTTATAGAC 
ACGACCAATG AGGTGATTGC TGACCGCGAC ACTGATATCG CCTACAACAA TCTGAAGCGG 
CAAGAACTTG AGTCTGAAAA CAAGAAGCTG AAAAATGACT TGAATGAGAT GAGGAAGACG 
ATAGCAGATG GAGCAGTAGA TGGTTCGAGT GAAGAGGCCA GAATTGCTAT GTTACTCAAC 
CAACTGAAGT CGGCCCACGA GGAGCTGGAT GTGCGGAAGG AAGAAGTTCT TATGCTCAGA 
ACCCAGATTG TAAAGGCAGC TCAGCAAAAA GAATCAGAGA GGATTATGAG TTCAGAAGCC 
AGTTGGCCAA ACAGCAACAA GGAAATGGAT GAAGACGATG CCATTGAAGC CTATCATGGA 
GTCTGTGAGA CAAACAGAAA GACTCAGGAC TGGGGGTATC TAAATGAAGA TGGGGAGCTT 
GGCCTGGCCT ATCAAGGCTT AAAGCAAGTT GCCAGGCTTC TGGAGGCTCA GCTTCAGGCA 
CAAAGCAGGC AGCACGATGA GGAGGTGGAG ATACTGACCT CTCACATTGA CTCCCTAAAG 
GAAGAGTTGG AGAAGCAGCA GCAGGTTCTA CTCCAAGCTC TTCAGCTCTC CCCAGAGGCT 
CAGGTGGAGT TTGGGCTTCA GCATGAAATT ACCCGCCTCA CTAATGAGAA CCTGGACCTT 
AAAGAATTAT TGGAGAAATC AGAAAAGAAT GAGAAAAAAT TGAAGAAACA GCTAAAAACG 
TACATGAAGA AAGGACAGGA TTCTGAAGAT GTAGTGCAGA CGGAGAGGAA ACCTGAGCTC 
ACCCGTCAAG TGACAGTGCA GCGAATTGAG AAGGATTTCC AAGGAATGCT GGAGTATTAC 
AAGGAGGAAG AACCGCTGCT CGTGAGAAAT CTCATTATAG ACGTGAAGCC TGAGCAGATG 
TCTTCCATGG TTCCTTGTCT GCCTGCGTAT ATCCTCTTCA TGTGCATCAG GCACGCCGAC 
TACATCAACG ATGATCAGAA GGTCCACTCC CTGCTCACCT CCACCATCAA CGGGATAAAG 
AAAGTTCTAA AGAAGCATCA CAGTGAATTC CAGATTACAT CTTTCTGGCT GTCCAACTCC 
AGCCGGCTGC TCCATTGCCT GAAACAGTAT AGTGGGGACG AGGGCTTCAT GACCAGCAAT 
AGCCCCAAGC AGAACGAGCA TTGCCTGAGG AACTTTGATC TGACAGAATA CCGCCAGGTC 
CTCAGCGACT TGTCTATCCA GATCTACCAG CAGATGATAA AGATTGCGGA AACGCATATG 
CAGCCAATGA TAGTGTCTGC CATGTTGGAA ACAGAAAGCA TCCAAGGCCT CTCAGGAGTG 
AAGCAAACTG GGTACAGGAA GAGGACCTCC AGCATGGCAG ATGGGGATAA TTCCTACAGC 
CTAGAAGCTG TCATTCGCCA GCTGAACACG TTTAACAGCA TCATGTGTGA CCATGGACTG 
GATCCCGAAA TCATTCAGCA AGTCTTTAAG CAGCTCTTCT ACATGATAAA TGCTGTAACG 
CTGAATAATC TGCTGCTGAG GAAGGACGTT TGCTCATGGA GCACCGGCAT GCAGCTAAGG 
TATAATATCA GTCAGTTAGA AGAATGGCTG CGAGGGAAGA ATTTGCATCC GAGTGGCGCT 
GCTCAGACTA TGGAGCCGCT GATCCAAGCA GCACAGTTAC TGCAACTCAA AAAGAAGACA 
GAGGAGGATG CAGAGGCCAT ATGCTCTTTG TGCACAGCCC TGACGACCCA GCAGATTGTG 
AAGATACTGA ATCTGTACAC ACCCGTGAAT GAGTTTGAAG AGCGAGTAAC CGTGTCCTTT 
ATCAGAATCA TTCAGGCTCA GCTGCAAGAG CGGAACGATC CTCCCCAGCT GCTAATGGAC 
TCTAAGTACA TGTTCCCAGT CCTGTTCCCA TTCAGCCCCT CGGCCATCAC TTTGGACACC 
ATTCAGATCC CTCCTTCCCT CAAACTGGAT TTCCTCATGA AGGTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

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1-877-436-7274

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myo5b cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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PHONE

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