tns1 cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

Gene
Clones
gRNAs

Summary

Gene Symbol	tns1
Entrez Gene ID	100216306
Full Name	tensin 1
Synonyms	mst091,mxra6,tensin,tns
General protein information	Preferred Names tensin 1 Names tensin-1
Gene Type	protein-coding
Organism	Xenopus tropicalis(tropical clawed frog)

mRNA and Protein(s)

mRNA	Protein	Name
XM_012970925.2	XP_012826379.1	tensin-1
XM_031893769.1	XP_031749629.1	tensin-1 isoform X1
XM_031893771.1	XP_031749631.1	tensin-1 isoform X3
XM_031893772.1	XP_031749632.1	tensin-1 isoform X4
XM_031893770.1	XP_031749630.1	tensin-1 isoform X2
XM_031893773.1	XP_031749633.1	tensin-1 isoform X5
XM_031893774.1	XP_031749634.1	tensin-1 isoform X6

Pathways from BioSystems

Homologies

Macaca mulatta (Rhesus monkey)	LOC696960	XP_002808070.1
Bos taurus (cattle)	TNS1	NP_777191.1
Danio rerio (zebrafish)	tns1	XP_003199292.2
Xenopus tropicalis (tropical clawed frog)	tns1	XP_004918285.1
Homo sapiens (human)	TNS1	NP_072174.3
Pan troglodytes (chimpanzee)	TNS1	XP_516080.3
Mus musculus (house mouse)	Tns1	NP_082160.3
Canis lupus familiaris (dog)	TNS1	XP_005640681.1
Gallus gallus (chicken)	TNS1	NP_990786.1
Rattus norvegicus (Norway rat)	Tns1	NP_001178739.1

Gene Ontology
Bibliography

Related articles in PubMed

Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences.
Strausberg RL, Feingold EA, Grouse LH, Derge JG, Klausner RD, Collins FS, Wagner L, Shenmen CM, Schuler GD, Altschul SF, Zeeberg B, Buetow KH, Schaefer CF, Bhat NK, Hopkins RF, Jordan H, Moore T, Max SI, Wang J, Hsieh F, Diatchenko L, Marusina K, Farmer AA, Rubin GM, Hong L, Stapleton M, Soares MB, Bonaldo MF, Casavant TL, Scheetz TE, Brownstein MJ, Usdin TB, Toshiyuki S, Carninci P, Prange C, Raha SS, Loquellano NA, Peters GJ, Abramson RD, Mullahy SJ, Bosak SA, McEwan PJ, McKernan KJ, Malek JA, Gunaratne PH, Richards S, Worley KC, Hale S, Garcia AM, Gay LJ, Hulyk SW, Villalon DK, Muzny DM, Sodergren EJ, Lu X, Gibbs RA, Fahey J, Helton E, Ketteman M, Madan A, Rodrigues S, Sanchez A, Whiting M, Madan A, Young AC, Shevchenko Y, Bouffard GG, Blakesley RW, Touchman JW, Green ED, Dickson MC, Rodriguez AC, Grimwood J, Schmutz J, Myers RM, Butterfield YS, Krzywinski MI, Skalska U, Smailus DE, Schnerch A, Schein JE, Jones SJ, Marra MA,
Proceedings of the National Academy of Sciences of the United States of America99(26)16899-903(2002 Dec)

Genetic and genomic tools for Xenopus research: The NIH Xenopus initiative.
Klein SL, Strausberg RL, Wagner L, Pontius J, Clifton SW, Richardson P
Developmental dynamics : an official publication of the American Association of Anatomists225(4)384-91(2002 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following tns1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the tns1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OXa32793	XM_012970925.2 Latest version!	Xenopus tropicalis tensin 1 (tns1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OXa67401	XM_031893769.1 Latest version!	Xenopus tropicalis tensin 1 (tns1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OXa67402	XM_031893770.1 Latest version!	Xenopus tropicalis tensin 1 (tns1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OXa67403	XM_031893771.1 Latest version!	Xenopus tropicalis tensin 1 (tns1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OXa67404	XM_031893772.1 Latest version!	Xenopus tropicalis tensin 1 (tns1), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OXa67405	XM_031893773.1 Latest version!	Xenopus tropicalis tensin 1 (tns1), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OXa67406	XM_031893774.1 Latest version!	Xenopus tropicalis tensin 1 (tns1), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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2-4 Clone	40% OFF
5 or more Clone	50% OFF
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

tns1 ( XM_012970925.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012826379.1 Xenopus tropicalis tensin 1 (tns1), mRNA.

Price & Availability↑

CloneID	OXa32793
Clone ID Related Accession (Same CDS sequence)	XM_012970925.2
Accession Version	XM_012970925.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5475bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1473177600000
Organism	Xenopus tropicalis(tropical clawed frog)
Product	tensin-1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_016683451.1) and transcript sequence (BC169188.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012970925.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## assembly gap :: added 117 transcript bases to patch genome assembly gap ##RefSeq-Attributes-END##

ATGGGATGCA CCGTCAGCTT CATCTGCTGT GAAGAGGACC CCCCGCTCAT GTCTGCTGAG 
CCCCAACATG AGGCCGAAAC GAAGGAGGAA AAAAAGAGGC TCCTTCCCAA GCCTGAAGAC 
CTGGAAGGGC AGAACCTTCA TACATTCAAG GTGAAGACCT TTAAGAAGGT GAAATCGTGC 
AGCGTGTGCA AGCAGGCCAT CACCAGAGAG GGCAGTGCGT GCCGGGTTTG CCGATTGTCG 
TGCCACAGAA AATGTGAAGC CAAGATTGCG TCGCCCTGTA CTCCTCGGGT CAATTATGAG 
CTGCCGTCAA GCTCTGAAAG CCCCCAGAAG AATGTAGAAA CCACAGGATC CACCAAATCC 
TCCAGAAGCA CTCAGTCCCG CCGCAGACCA TCAAGGAGCA TTAGCCTGAT TCACGCCATG 
GAGGACAGCT GCGAGTTGGA CCTCACCTAC ATCACCGAGC GGATCATCTC CGTCACCTTC 
TCCACCGGGA CGGAAGAGCC CACCTTCTGC CGGAACCTGA AGGAAGTGGC GCATATGCTG 
AAGTCCAAAC ATGGAGACAA TTACCTGATC TTCAACTTGT CGGAACGCAG ACATGACATC 
AGCAAGCTCC ATTCCAAGGT GTTGGATTTC GGATGGCCGG ACCTGCACGC GCCGGCCTTG 
GAAAAAGTCT GCAGCATCTG CAAAGCCATG GACACCTGGC TCAACGCCGA CCCTCACAAC 
GTCGTCGTCA TCCATAACAA AGGCAACCGA GGGCGGACAG GTGTGGTGAT TGCCGCCTAT 
ATGCACTACA GCAACATATC TGCCAGTGCA GACCAAGCCT TGGACAGGTT TGCCATGAGA 
AGGTTCTATG AAGACAAAGT CCTTCCAGTA GCTCAACCTT CCCAAAAAAG GTATATAAAC 
TACTTCAGTG GGCTTCTCTC TGGCGCCATG AAAATGAACA GCAAACCTCT CTTCCTGCAT 
CATGTGATCA TGCATGGGAT CCCCAACTTT GAGTCCAAGG GAGGTTGTCG GCCATTTCTG 
AAGATCTACC AAGCGATGCA GCCTGTATAC ACCTCGGGCA TCTACAACGT GCAAGGGGAC 
AGCCAGACGA GCATCTGCAT CACCATAGAA CCGGGGCTGT TACTAAAAGG TGACATCTTG 
CTCAAGTGCT ACCACAAGAA GTTCCGCAGC CCCACGCGGG ACGTCATATT CCGGGTGCAG 
TTCCATACGT GCGCCATCCA TGATCTGGGA ATCGTGTTTG GGAAGGAGGA TCTGGACGAA 
GCTTTCAAAG ATGAGCGTTT TCCCGAATAT GGAAAAGTGG AATTTGTTTT TTCATATGGA 
CCAGAAAAGA TACAAGGCAT GGAGCACCTG GAGAATGGAC CCAGCGTCTC TGTGGATTAT 
AACACATCCG ACCCTCTGAT TCGCTGGGAC TCCTATGATA ACTTCAACGT GCACCGGGAA 
GACAGCCTGG AAGAGGTTGG ACACACTCAA GGCCCCTTGG ATGGAAGCCT GTACGCCAAG 
GTGAAGAAGA AAGACTCTAC GAACGGCAGC ACCAGCACCG TCAACGCCAG CGGAATTCCG 
TTGTCTGCCG CGCCCAATCA CATTGAACAC ACCCTGTCCG TGAGCAGCGA CTCCGGCAAC 
TCGACTGCTT CGACCAAAAC CGACAAGACC GATGAGCAAG TGGCCACGGC GGCTGCCAAC 
CAAGGGTTGA CCCCTGAGGA GAAGCAAGAG TTGGAGCGGC TCCTGAGCGG CTTTGGCTTG 
GAGACCGAAG GGCAGATGCA CAATTTAAAT CCGGGTACAT CTGCAGCAGC AGCGATGCTT 
CACGTTGTGC CGGCGCAAGT CCACGTCAAT GGGGAACGGA CATCTCCGCA GGTGGAACGA 
GAGACAGATA TTCTTGATGA TGATCTGCCC AACCATGATG TTCACAGTGT TGGAAGCTTG 
GGGACCCTGT CTTCGTGTGA TGTGATTCCA AGCTTGAGTG AGACTGGCTA CCAGGATTCT 
CACCTTCGCA TGTCCCCCTT GACCAATGGT CCAGCCACTG TTAATGGCAT TGATAACCTG 
CAAAAACATG GGTTTATTGG TAAAGAGACG ATGATTAATG GCGGCTACCC GTACAACAAC 
CAGAATGTCT TAAAAAGCGC TTTGATCCCT CATTCCCAGG ATGCAGGGAT CCATATGCGA 
CCTTCCGTGT CTGCTCATGA AAACTTAGCC TATCAAGTCA ACCAGCCCAA CTGGGGACAA 
CCTTCTCCAT CAAATGTAAC ACAAAAGCAC TTATACTATT ATGACCCGAA TGGCATGTAC 
CGTTCACAGT CGTACACCGC TGCCGAACCA GGGAAATTCG AAAACATTTC TCAACTACCC 
CAAGCTCCAG CCCGTAGCGT TAGTAGCAGA GAGGCGGTGC AGAGGGGCCT TAACTCGTGG 
CAGCAAAGTG GAAGCAGGCC CCCATCCCGG GATGGAACTA CTGAGAATCA AGTTGCCAGT 
CCTAGCCCGC AGCCAAGCAC TTTCCAGACT GTTCCACAGA GCCACAGCAT CCCAGAATTC 
CCCAAAATGG CTTCCCAGAA GGAGATTGAG CAGTCTATAG AAGCACTTAA TATGCTTATG 
CTGGACCTGG ACCCTTCATT CTCACAAATG CACAAGTCTC AGAGTGTGCC TGTTGCCCCT 
AGAGAAGAAA GGGTTCTGCC AGTGCAAGGA TCCTTCTCAG CGCAGCCATC TTTGGGCATT 
TATAGCCAGC CGACTCCACA GGTGGCTACA CAAAGGCCTA GCAACGCTTA CGTGGCTCCT 
AGCATGGTGC AGAGCGCTGG AGCCTCTCAA AGCCAACCAC ACTTGCCTTC GGAACAGGTT 
TCGAGAACAT ATTCACCTGG GGTGCATCAT GACCAAGCTC GGGCTGTTGG GCCAGAAAAA 
GTTCTGAATG ACTATCGGGA GCCTTATGCT GCTGGAACCT TCTCTCCTTA TGGTTATCAG 
AACCAACAAC CGCCTTTGGC ATTGACAAGA AGCTATAGCT ATGGAAGTAG TGCTTCGCCA 
TCTTCGGTTC CACCATTATC GGCATATATG CAGAGCAATA CTCAGCAACC CGTCCTTTCT 
TCTCCTGCTT CTTCTGTGGT CATGGCTCAA CCTCAGCCTC AATCCCAGTC TCCGACCAGA 
GAGCCAGACG ATGAACAGTA CAGTCTGGAA GGGCTGGTAG CTCACAGAAT TGCTGGGGTG 
CACACTCGGG ACAAGACTCC GGATGAGATC GGGTCCCTGG CACGGAGGCG CACCACCAGC 
GAGGGGCAAT ATGACGAGGG CAATGCTGAC CTGGGCAGAG CGAGTAGCCC CACCAAGGTG 
CGCTCCCCAG TGCGCTGCGT CTCCCCGGAA GTCGCCTTCA CCATTGCGGC AAACCCAGGG 
GGGAGACCCA AGGAGCCCCA TCTGCACAGC TACAAGGAGG CCTTTGAAGA GATGGAAATG 
ACGCCGCCAG TGATCAGCCC GGGGGCCACA GTAGCCCGAT CCCCCCCTGG TTTAGCCAAG 
ACCCCCCTGT CTGCCCTGGG ACTGAAGCCC CACAATCCCG CAGAAATCGT TCTCCAGCAA 
TCGGGAGAGC CCAGGAGTTA TGTTGAATCC GTTGCCCGGA CGGCCGCCGC GGGAGGAGTC 
GCACCCCCAT CACCTACGGG CCCCCAGGCA TATAGTGTCG ATTCTCTGAT GAGAAATGGG 
GGTTACCCCA ATGTTTTCAC CTCTCCCAGC CCCATTTCTA CCAGCAGCCC CATACACAGC 
ACTGATGGCT CCCTAAGGAG TTACCCGTCG GTGGAGAGCG GCGCCCGTGT CCCCACTCCC 
ACTCAGCCTT CCGTGGATTC CAGCTTCAGA TCCACCAGTC TATCGCAGCC GTCGCCCCAA 
AGCAGTTACC AGAACGCCTC TCCCATGGTT CCCTACGCTG GCACCCCGGC CAGTTCCTAT 
GATGGCTACC CTTATTCCCC CAGCAGTGCC CTCACCCAGA CAGACGGCCT CTCCGGGCCA 
CAGATGAACG CGGTCATTGG TCACATGGTT CACGGAAGCC CCAAGGCCGT TCATAGAACG 
GTGGGGACCA ACACACCACC GAGTCCAGGT TTTGGGAGGA GAATGGTCAC CAACAATATG 
GCGGTTCCAC CCGGAAGTCC AAGCATGGGC AGACACCCCA TGGCTACACA TATGGCTGCA 
CCCGTGACAG TAGGTAGCCC CAGCCTTCCT CTCCACCAGG CCTTGATGAC GGGAAGTGCT 
CCGGTGACTC CTGGTAGCCC CGGCATGGAG AGACACGTTA TGTATGGCTA TTCCCCCGCT 
GATGACAAAC GACCGACACT GTCACGGCAG AGCAGTGCGT CCGGATACCA GGCACCGCCC 
ACCCCATCCT TCCCGGTCTC CCCGGCCTAT TACCATGGCA TGAGTAGCCC TTCCTCCTCC 
TCTCCCGACT CAACCTTATA CCGGCAAGGG AGCCCAGTCT CGCAGCCGTC TCTGCCCGAG 
AAGAGGAGAA TGTCATCTGG GGACAGATCC AACAGCCTCC CCAACTACTC CACCCTCAAT 
GGAAAAACCT CTTCGCCCAT GTCCAGCGGG ATGTCCAGCC CCAGCGGCGG AAGCACAATG 
GCATTTACTC ACACTCTACC GGACTTCTCC AAGTTCTCCA TGCCAGACAG CAGCCCGGAG 
ACCCGAGCAA ATGTGAAGTT TGTGCAGGAC ACGTCCAAGT ACTGGTACAA ACCAGACATT 
TCCAGGGATC AGGCCATCTC CCTGCTGAAG GACAAAGACC CCGGGGCGTT CATCATACGA 
GACAGCCATT CGTTCCGAGG GGCCTACGGA CTTGCCATGA AAGTAGCCAC CCCGCCCCCT 
AATGCTGGCC AGCCCAGCAA GAAGGGAGAT GTGACCAATG AGCTGGTTCG GCACTTTTTG 
ATTGAGACGA GTCCCAGGGG GGTGAAATTA AAGGGCTGCC AGAACGAACC CTACTTTGGC 
TGCCTGTCGG CTCTGGTGTA CCAGCACTCC ATTACTCCCC TTGCACTGCC CTGCAAACTG 
GTCATACCCT GCAGAGATCC CTCTGATGAG ACCAAGGAGA CGGCTGTTCC GGCCAATTCA 
GCCTCAGATC TACTGAAACA GGGCGCAGCC TGCAACGTGT TATTCATAAA CTCGATAGAC 
ATGGAGTCGC TGACCGGCCC ACAAGCCATT GCGAAGACCA TCGCCGAGTC TCTCGCCGCC 
GACCCCCCGC CGGCCGCCGC CATCGTTCAC TTCAAAGTCT CCGCCCAAGG AATCACCTTA 
ACCGACAACC AGAGAAAATT ATTTTTCAGA CGACATTATC CGCTCAGCTC AGTCACCTTC 
TGCGACCTGG ACCCGCAAGA GAGAAAGTGG ATGAAGGCCG ATGGGACACA AGCCAAACTC 
TTTGGCTTTG TGGCCAGAAA GCAAGGAAGC ACCACAGACA ATATGTGCCA CCTGTTTGCC 
GAACTTGACC CAAACCAACC AGCCACTGCC ATTGTCAACT TTGTGTCCCG AGTCATCGGC 
CTGCAGAAGA GATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012826379.1
CDS	169..5643
Translation	MGCTVSFICCEEDPPLMSAEPQHEAETKEEKKRLLPKPEDLEGQNLHTFKVKTFKKVKSCSVCKQAITREGSACRVCRLSCHRKCEAKIASPCTPRVNYELPSSSESPQKNVETTGSTKSSRSTQSRRRPSRSISLIHAMEDSCELDLTYITERIISVTFSTGTEEPTFCRNLKEVAHMLKSKHGDNYLIFNLSERRHDISKLHSKVLDFGWPDLHAPALEKVCSICKAMDTWLNADPHNVVVIHNKGNRGRTGVVIAAYMHYSNISASADQALDRFAMRRFYEDKVLPVAQPSQKRYINYFSGLLSGAMKMNSKPLFLHHVIMHGIPNFESKGGCRPFLKIYQAMQPVYTSGIYNVQGDSQTSICITIEPGLLLKGDILLKCYHKKFRSPTRDVIFRVQFHTCAIHDLGIVFGKEDLDEAFKDERFPEYGKVEFVFSYGPEKIQGMEHLENGPSVSVDYNTSDPLIRWDSYDNFNVHREDSLEEVGHTQGPLDGSLYAKVKKKDSTNGSTSTVNASGIPLSAAPNHIEHTLSVSSDSGNSTASTKTDKTDEQVATAAANQGLTPEEKQELERLLSGFGLETEGQMHNLNPGTSAAAAMLHVVPAQVHVNGERTSPQVERETDILDDDLPNHDVHSVGSLGTLSSCDVIPSLSETGYQDSHLRMSPLTNGPATVNGIDNLQKHGFIGKETMINGGYPYNNQNVLKSALIPHSQDAGIHMRPSVSAHENLAYQVNQPNWGQPSPSNVTQKHLYYYDPNGMYRSQSYTAAEPGKFENISQLPQAPARSVSSREAVQRGLNSWQQSGSRPPSRDGTTENQVASPSPQPSTFQTVPQSHSIPEFPKMASQKEIEQSIEALNMLMLDLDPSFSQMHKSQSVPVAPREERVLPVQGSFSAQPSLGIYSQPTPQVATQRPSNAYVAPSMVQSAGASQSQPHLPSEQVSRTYSPGVHHDQARAVGPEKVLNDYREPYAAGTFSPYGYQNQQPPLALTRSYSYGSSASPSSVPPLSAYMQSNTQQPVLSSPASSVVMAQPQPQSQSPTREPDDEQYSLEGLVAHRIAGVHTRDKTPDEIGSLARRRTTSEGQYDEGNADLGRASSPTKVRSPVRCVSPEVAFTIAANPGGRPKEPHLHSYKEAFEEMEMTPPVISPGATVARSPPGLAKTPLSALGLKPHNPAEIVLQQSGEPRSYVESVARTAAAGGVAPPSPTGPQAYSVDSLMRNGGYPNVFTSPSPISTSSPIHSTDGSLRSYPSVESGARVPTPTQPSVDSSFRSTSLSQPSPQSSYQNASPMVPYAGTPASSYDGYPYSPSSALTQTDGLSGPQMNAVIGHMVHGSPKAVHRTVGTNTPPSPGFGRRMVTNNMAVPPGSPSMGRHPMATHMAAPVTVGSPSLPLHQALMTGSAPVTPGSPGMERHVMYGYSPADDKRPTLSRQSSASGYQAPPTPSFPVSPAYYHGMSSPSSSSPDSTLYRQGSPVSQPSLPEKRRMSSGDRSNSLPNYSTLNGKTSSPMSSGMSSPSGGSTMAFTHTLPDFSKFSMPDSSPETRANVKFVQDTSKYWYKPDISRDQAISLLKDKDPGAFIIRDSHSFRGAYGLAMKVATPPPNAGQPSKKGDVTNELVRHFLIETSPRGVKLKGCQNEPYFGCLSALVYQHSITPLALPCKLVIPCRDPSDETKETAVPANSASDLLKQGAACNVLFINSIDMESLTGPQAIAKTIAESLAADPPPAAAIVHFKVSAQGITLTDNQRKLFFRRHYPLSSVTFCDLDPQERKWMKADGTQAKLFGFVARKQGSTTDNMCHLFAELDPNQPATAIVNFVSRVIGLQKR

Target ORF information:

RefSeq Version	XM_012970925.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis tensin 1 (tns1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012970925.2

ORF Insert Sequence:

ATGGGATGCA CCGTCAGCTT CATCTGCTGT GAAGAGGACC CCCCGCTCAT GTCTGCTGAG 
CCCCAACATG AGGCCGAAAC GAAGGAGGAA AAAAAGAGGC TCCTTCCCAA GCCTGAAGAC 
CTGGAAGGGC AGAACCTTCA TACATTCAAG GTGAAGACCT TTAAGAAGGT GAAATCGTGC 
AGCGTGTGCA AGCAGGCCAT CACCAGAGAG GGCAGTGCGT GCCGGGTTTG CCGATTGTCG 
TGCCACAGAA AATGTGAAGC CAAGATTGCG TCGCCCTGTA CTCCTCGGGT CAATTATGAG 
CTGCCGTCAA GCTCTGAAAG CCCCCAGAAG AATGTAGAAA CCACAGGATC CACCAAATCC 
TCCAGAAGCA CTCAGTCCCG CCGCAGACCA TCAAGGAGCA TTAGCCTGAT TCACGCCATG 
GAGGACAGCT GCGAGTTGGA CCTCACCTAC ATCACCGAGC GGATCATCTC CGTCACCTTC 
TCCACCGGGA CGGAAGAGCC CACCTTCTGC CGGAACCTGA AGGAAGTGGC GCATATGCTG 
AAGTCCAAAC ATGGAGACAA TTACCTGATC TTCAACTTGT CGGAACGCAG ACATGACATC 
AGCAAGCTCC ATTCCAAGGT GTTGGATTTC GGATGGCCGG ACCTGCACGC GCCGGCCTTG 
GAAAAAGTCT GCAGCATCTG CAAAGCCATG GACACCTGGC TCAACGCCGA CCCTCACAAC 
GTCGTCGTCA TCCATAACAA AGGCAACCGA GGGCGGACAG GTGTGGTGAT TGCCGCCTAT 
ATGCACTACA GCAACATATC TGCCAGTGCA GACCAAGCCT TGGACAGGTT TGCCATGAGA 
AGGTTCTATG AAGACAAAGT CCTTCCAGTA GCTCAACCTT CCCAAAAAAG GTATATAAAC 
TACTTCAGTG GGCTTCTCTC TGGCGCCATG AAAATGAACA GCAAACCTCT CTTCCTGCAT 
CATGTGATCA TGCATGGGAT CCCCAACTTT GAGTCCAAGG GAGGTTGTCG GCCATTTCTG 
AAGATCTACC AAGCGATGCA GCCTGTATAC ACCTCGGGCA TCTACAACGT GCAAGGGGAC 
AGCCAGACGA GCATCTGCAT CACCATAGAA CCGGGGCTGT TACTAAAAGG TGACATCTTG 
CTCAAGTGCT ACCACAAGAA GTTCCGCAGC CCCACGCGGG ACGTCATATT CCGGGTGCAG 
TTCCATACGT GCGCCATCCA TGATCTGGGA ATCGTGTTTG GGAAGGAGGA TCTGGACGAA 
GCTTTCAAAG ATGAGCGTTT TCCCGAATAT GGAAAAGTGG AATTTGTTTT TTCATATGGA 
CCAGAAAAGA TACAAGGCAT GGAGCACCTG GAGAATGGAC CCAGCGTCTC TGTGGATTAT 
AACACATCCG ACCCTCTGAT TCGCTGGGAC TCCTATGATA ACTTCAACGT GCACCGGGAA 
GACAGCCTGG AAGAGGTTGG ACACACTCAA GGCCCCTTGG ATGGAAGCCT GTACGCCAAG 
GTGAAGAAGA AAGACTCTAC GAACGGCAGC ACCAGCACCG TCAACGCCAG CGGAATTCCG 
TTGTCTGCCG CGCCCAATCA CATTGAACAC ACCCTGTCCG TGAGCAGCGA CTCCGGCAAC 
TCGACTGCTT CGACCAAAAC CGACAAGACC GATGAGCAAG TGGCCACGGC GGCTGCCAAC 
CAAGGGTTGA CCCCTGAGGA GAAGCAAGAG TTGGAGCGGC TCCTGAGCGG CTTTGGCTTG 
GAGACCGAAG GGCAGATGCA CAATTTAAAT CCGGGTACAT CTGCAGCAGC AGCGATGCTT 
CACGTTGTGC CGGCGCAAGT CCACGTCAAT GGGGAACGGA CATCTCCGCA GGTGGAACGA 
GAGACAGATA TTCTTGATGA TGATCTGCCC AACCATGATG TTCACAGTGT TGGAAGCTTG 
GGGACCCTGT CTTCGTGTGA TGTGATTCCA AGCTTGAGTG AGACTGGCTA CCAGGATTCT 
CACCTTCGCA TGTCCCCCTT GACCAATGGT CCAGCCACTG TTAATGGCAT TGATAACCTG 
CAAAAACATG GGTTTATTGG TAAAGAGACG ATGATTAATG GCGGCTACCC GTACAACAAC 
CAGAATGTCT TAAAAAGCGC TTTGATCCCT CATTCCCAGG ATGCAGGGAT CCATATGCGA 
CCTTCCGTGT CTGCTCATGA AAACTTAGCC TATCAAGTCA ACCAGCCCAA CTGGGGACAA 
CCTTCTCCAT CAAATGTAAC ACAAAAGCAC TTATACTATT ATGACCCGAA TGGCATGTAC 
CGTTCACAGT CGTACACCGC TGCCGAACCA GGGAAATTCG AAAACATTTC TCAACTACCC 
CAAGCTCCAG CCCGTAGCGT TAGTAGCAGA GAGGCGGTGC AGAGGGGCCT TAACTCGTGG 
CAGCAAAGTG GAAGCAGGCC CCCATCCCGG GATGGAACTA CTGAGAATCA AGTTGCCAGT 
CCTAGCCCGC AGCCAAGCAC TTTCCAGACT GTTCCACAGA GCCACAGCAT CCCAGAATTC 
CCCAAAATGG CTTCCCAGAA GGAGATTGAG CAGTCTATAG AAGCACTTAA TATGCTTATG 
CTGGACCTGG ACCCTTCATT CTCACAAATG CACAAGTCTC AGAGTGTGCC TGTTGCCCCT 
AGAGAAGAAA GGGTTCTGCC AGTGCAAGGA TCCTTCTCAG CGCAGCCATC TTTGGGCATT 
TATAGCCAGC CGACTCCACA GGTGGCTACA CAAAGGCCTA GCAACGCTTA CGTGGCTCCT 
AGCATGGTGC AGAGCGCTGG AGCCTCTCAA AGCCAACCAC ACTTGCCTTC GGAACAGGTT 
TCGAGAACAT ATTCACCTGG GGTGCATCAT GACCAAGCTC GGGCTGTTGG GCCAGAAAAA 
GTTCTGAATG ACTATCGGGA GCCTTATGCT GCTGGAACCT TCTCTCCTTA TGGTTATCAG 
AACCAACAAC CGCCTTTGGC ATTGACAAGA AGCTATAGCT ATGGAAGTAG TGCTTCGCCA 
TCTTCGGTTC CACCATTATC GGCATATATG CAGAGCAATA CTCAGCAACC CGTCCTTTCT 
TCTCCTGCTT CTTCTGTGGT CATGGCTCAA CCTCAGCCTC AATCCCAGTC TCCGACCAGA 
GAGCCAGACG ATGAACAGTA CAGTCTGGAA GGGCTGGTAG CTCACAGAAT TGCTGGGGTG 
CACACTCGGG ACAAGACTCC GGATGAGATC GGGTCCCTGG CACGGAGGCG CACCACCAGC 
GAGGGGCAAT ATGACGAGGG CAATGCTGAC CTGGGCAGAG CGAGTAGCCC CACCAAGGTG 
CGCTCCCCAG TGCGCTGCGT CTCCCCGGAA GTCGCCTTCA CCATTGCGGC AAACCCAGGG 
GGGAGACCCA AGGAGCCCCA TCTGCACAGC TACAAGGAGG CCTTTGAAGA GATGGAAATG 
ACGCCGCCAG TGATCAGCCC GGGGGCCACA GTAGCCCGAT CCCCCCCTGG TTTAGCCAAG 
ACCCCCCTGT CTGCCCTGGG ACTGAAGCCC CACAATCCCG CAGAAATCGT TCTCCAGCAA 
TCGGGAGAGC CCAGGAGTTA TGTTGAATCC GTTGCCCGGA CGGCCGCCGC GGGAGGAGTC 
GCACCCCCAT CACCTACGGG CCCCCAGGCA TATAGTGTCG ATTCTCTGAT GAGAAATGGG 
GGTTACCCCA ATGTTTTCAC CTCTCCCAGC CCCATTTCTA CCAGCAGCCC CATACACAGC 
ACTGATGGCT CCCTAAGGAG TTACCCGTCG GTGGAGAGCG GCGCCCGTGT CCCCACTCCC 
ACTCAGCCTT CCGTGGATTC CAGCTTCAGA TCCACCAGTC TATCGCAGCC GTCGCCCCAA 
AGCAGTTACC AGAACGCCTC TCCCATGGTT CCCTACGCTG GCACCCCGGC CAGTTCCTAT 
GATGGCTACC CTTATTCCCC CAGCAGTGCC CTCACCCAGA CAGACGGCCT CTCCGGGCCA 
CAGATGAACG CGGTCATTGG TCACATGGTT CACGGAAGCC CCAAGGCCGT TCATAGAACG 
GTGGGGACCA ACACACCACC GAGTCCAGGT TTTGGGAGGA GAATGGTCAC CAACAATATG 
GCGGTTCCAC CCGGAAGTCC AAGCATGGGC AGACACCCCA TGGCTACACA TATGGCTGCA 
CCCGTGACAG TAGGTAGCCC CAGCCTTCCT CTCCACCAGG CCTTGATGAC GGGAAGTGCT 
CCGGTGACTC CTGGTAGCCC CGGCATGGAG AGACACGTTA TGTATGGCTA TTCCCCCGCT 
GATGACAAAC GACCGACACT GTCACGGCAG AGCAGTGCGT CCGGATACCA GGCACCGCCC 
ACCCCATCCT TCCCGGTCTC CCCGGCCTAT TACCATGGCA TGAGTAGCCC TTCCTCCTCC 
TCTCCCGACT CAACCTTATA CCGGCAAGGG AGCCCAGTCT CGCAGCCGTC TCTGCCCGAG 
AAGAGGAGAA TGTCATCTGG GGACAGATCC AACAGCCTCC CCAACTACTC CACCCTCAAT 
GGAAAAACCT CTTCGCCCAT GTCCAGCGGG ATGTCCAGCC CCAGCGGCGG AAGCACAATG 
GCATTTACTC ACACTCTACC GGACTTCTCC AAGTTCTCCA TGCCAGACAG CAGCCCGGAG 
ACCCGAGCAA ATGTGAAGTT TGTGCAGGAC ACGTCCAAGT ACTGGTACAA ACCAGACATT 
TCCAGGGATC AGGCCATCTC CCTGCTGAAG GACAAAGACC CCGGGGCGTT CATCATACGA 
GACAGCCATT CGTTCCGAGG GGCCTACGGA CTTGCCATGA AAGTAGCCAC CCCGCCCCCT 
AATGCTGGCC AGCCCAGCAA GAAGGGAGAT GTGACCAATG AGCTGGTTCG GCACTTTTTG 
ATTGAGACGA GTCCCAGGGG GGTGAAATTA AAGGGCTGCC AGAACGAACC CTACTTTGGC 
TGCCTGTCGG CTCTGGTGTA CCAGCACTCC ATTACTCCCC TTGCACTGCC CTGCAAACTG 
GTCATACCCT GCAGAGATCC CTCTGATGAG ACCAAGGAGA CGGCTGTTCC GGCCAATTCA 
GCCTCAGATC TACTGAAACA GGGCGCAGCC TGCAACGTGT TATTCATAAA CTCGATAGAC 
ATGGAGTCGC TGACCGGCCC ACAAGCCATT GCGAAGACCA TCGCCGAGTC TCTCGCCGCC 
GACCCCCCGC CGGCCGCCGC CATCGTTCAC TTCAAAGTCT CCGCCCAAGG AATCACCTTA 
ACCGACAACC AGAGAAAATT ATTTTTCAGA CGACATTATC CGCTCAGCTC AGTCACCTTC 
TGCGACCTGG ACCCGCAAGA GAGAAAGTGG ATGAAGGCCG ATGGGACACA AGCCAAACTC 
TTTGGCTTTG TGGCCAGAAA GCAAGGAAGC ACCACAGACA ATATGTGCCA CCTGTTTGCC 
GAACTTGACC CAAACCAACC AGCCACTGCC ATTGTCAACT TTGTGTCCCG AGTCATCGGC 
CTGCAGAAGA GATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

tns1 ( XM_031893769.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_031749629.1 Xenopus tropicalis tensin 1 (tns1), transcript variant X1, mRNA.

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CloneID	OXa67401
Clone ID Related Accession (Same CDS sequence)	XM_031893769.1
Accession Version	XM_031893769.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5601bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1576512000000
Organism	Xenopus tropicalis(tropical clawed frog)
Product	tensin-1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030685.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGATGCA CCGTCAGCTT CATCTGCTGT GAAGAGGACC CCCCGCTCAT GTCTGCTGAG 
CCCCAACATG AGGCCGAAAC GAAGGAGGAA AAAAAGAGGC TCCTTCCCAA GCCTGAAGAC 
CTGGAAGGGC AGAACCTTCA TACATTCAAG GTGAAGACCT TTAAGAAGGT GAAATCGTGC 
AGCGTGTGCA AGCAGGCCAT CACCAGAGAG GGCAGTGCGT GCCGGGTTTG CCGATTGTCG 
TGCCACAGAA AATGTGAAGC CAAGATTGCG TCGCCCTGTA CTCCTCGGGT CAATTATGAG 
CTGCCGTCAA GCTCTGAAAG CCCCCAGAAG AATGTAGAAA CCACAGGATC CACCAAATCC 
TCCAGAAGCA CTCAGTCCCG CCGCAGACCA TCAAGGAGCA TTAGCCTGAT TCACGCCATG 
GAGGACAGCT GCGAGTTGGA CCTCACCTAC ATCACCGAGC GGATCATCTC CGTCACCTTC 
TCCACCGGGA CGGAAGAGCC CACCTTCTGC CGGAACCTGA AGGAAGTGGC GCATATGCTG 
AAGTCCAAAC ATGGAGACAA TTACCTGATC TTCAACTTGT CGGAACGCAG ACATGACATC 
AGCAAGCTCC ATTCCAAGGT GTTGGATTTC GGATGGCCGG ACCTGCACGC GCCGGCCTTG 
GAAAAAGTCT GCAGCATCTG CAAAGCCATG GACACCTGGC TCAACGCCGA CCCTCACAAC 
GTCGTCGTCA TCCATAACAA AGGCAACCGA GGGCGGACAG GTGTGGTGAT TGCCGCCTAT 
ATGCACTACA GCAACATATC TGCCAGTGCA GACCAAGCCT TGGACAGGTT TGCCATGAGA 
AGGTTCTATG AAGACAAAGT CCTTCCAGTA GCTCAACCTT CCCAAAAAAG GTATATAAAC 
TACTTCAGTG GGCTTCTCTC TGGCGCCATG AAAATGAACA GCAAACCTCT CTTCCTGCAT 
CATGTGATCA TGCATGGGAT CCCCAACTTT GAGTCCAAGG GAGGTTGTCG GCCATTTCTG 
AAGATCTACC AAGCGATGCA GCCTGTATAC ACCTCGGGCA TCTACAACGT GCAAGGGGAC 
AGCCAGACGA GCATCTGCAT CACCATAGAA CCGGGGCTGT TACTAAAAGG TGACATCTTG 
CTCAAGTGCT ACCACAAGAA GTTCCGCAGC CCCACGCGGG ACGTCATATT CCGGGTGCAG 
TTCCATACGT GCGCCATCCA TGATCTGGGA ATCGTGTTTG GGAAGGAGGA TCTGGACGAA 
GCTTTCAAAG ATGAGCGTTT TCCCGAATAT GGAAAAGTGG AATTTGTTTT TTCATATGGA 
CCAGAAAAGA TACAAGGCAT GGAGCACCTG GAGAATGGAC CCAGCGTCTC TGTGGATTAT 
AACACATCCG ACCCTCTGAT TCGCTGGGAC TCCTATGATA ACTTCAACGT GCACCGGGAA 
GACAGCCTGG AAGAGGTTGG ACACACTCAA GGCCCCTTGG ATGGAAGCCT GTACGCCAAG 
GTGAAGAAGA AAGACTCTAC GAACGGCAGC ACCAGCACCG TCAACGCCAG CGGAATTCCG 
TTGTCTGCCG CGCCCAATCA CATTGAACAC ACCCTGTCCG TGAGCAGCGA CTCCGGCAAC 
TCGACTGCTT CGACCAAAAC CGACAAGACC GATGAGCAAG TGGCCACGGC GGCTGCCAAC 
CAAGGGTTGA CCCCTGAGGA GAAGCAAGAG TTGGAGCGGC TCCTGAGCGG CTTTGGCTTG 
GAGACCGAAG GGCAGATGCA CAATTTAAAT CCGGGCACAT CTGCAGCAGC AGCGATGCTT 
CACGTTGTGC CGGCGCAAGT CCACGTCAAT GGGGAACGGA CATCTCCGCA GGTGGAACGA 
GAGACAGATA TTCTTGATGA TGATCTGCCC AACCATGATG TTCACAGTGT TGGAAGCTTG 
GGGACCCTGT CTTCGTGTGA TGTGATTCCA AGCTTGAGTG AGACTGGCTA CCAGGATTCT 
CACCTTCGCA TGTCCCCCTT GACCAATGGT CCAGCCACTG TTAATGGCAT TGATAACCTG 
CAAAAACATG GGTTTATTGG TAAAGAGACG ATGATTAATG GCGGCTACCC GTACAACAAC 
CAGAATGTCT TAAAAAGCGC TTTGATCCCT CATTCCCAGG ATGCAGGGAT CCATATGCGA 
CCTTCCGTGT CTGCTCATGA AAACTTAGCC TATCAAGTCA ACCAGCCCAA CTGGGGACAA 
CCTTCTCCAT CAAATGTAAC ACAAAAGCAC TTATACTATT ATGACCCGAA TGGCATGTAC 
CGTTCACAGT CGTACACCGC TGCCGAACCA GGGAAATTCG AAAACATTTC TCAACTACCC 
CAAGCTCCAG CCCGTAGCGT TAGTAGCAGA GAGGCGGTGC AGAGGGGCCT TAACTCGTGG 
CAGCAAAGTG GAAGCAGGCC CCCATCCCGG GATGGAACTA CTGAGAATCA AGTTGCCAGT 
CCTAGCCCGC AGCCAAGCAC TTTCCAGACT GTTCCACAGA GCCACAGCAT CCCAGAATTC 
CCCAAAATGG CTTCCCAGAA GGAGATTGAG CAGTCTATAG AAGCACTTAA TATGCTTATG 
CTGGACCTGG ACCCTTCATT CTCACAAATG CACAAGTCTC AGAGTGTGCC TGTTGCCCCT 
AGAGAAGAAA GGGTTCTGCC AGTGCAAGGA TCCTTCTCAG CGCAGCCATC TTTGGGCATT 
TATAGCCAGC CGACTCCACA GGTGGCTACA CAAAGGCCTA GCAACGCTTA CGTGGCTCCT 
AGCATGGTGC AGAGCGCTGG AGCCTCTCAA AGCCAACCAC ACTTGCCTTC GGAACAGGTT 
TCGAGAACAT ATTCACCTGG GGTGCATCAT GACCAAGCTC GGGCTGTTGG GCCAGAAAAA 
GTTCTGAATG ACTATCGGGA GCCTTATGCT GCTGGAACCT TCTCTCCTTA TGGTTATCAG 
AACCAACAAC CGCCTTTGGC ATTGACAAGA AGCTATAGCT ATGGAAGTAG TGCTTCGCCA 
TCTTCGGTTC CACCATTATC GGCATATATG CAGAGCAATA CTCAGCAACC CGTCCTTTCT 
TCTCCTGCTT CTTCTGTGGT CATGGCTCAA CCTCAGCCTC AATCCCAGTC TCCGACCAGA 
GAGCCAGACG ATGAACAGTA CAGTCTGGAA GGGCTGGTAG CTCACAGAAT TGCTGAGTAC 
AACAACAAAC TCCGGAATAT CCGTAAAAGC ATGAATAGCC CCCATCCCCA TGGCGTGCAG 
CGCCAGCGTT CCTTCACTTT CTCTGGGGTG CACACTCGGG ACAAGACTCC GGATGAGATC 
GGGTCCCTGG CACGGAGGCG CACCACCAGC GAGGGGCAAT ATGACGAGGG CAATGCTGAC 
CTGGGCAGAG CGAGTAGCCC CACCAAGGTG CGCTCCCCAG TGCGCTGCGT CTCCCCGGAA 
GTCGCCTTCA CCATTGCGGC AAACCCAGGG GGGAGACCCA AGGAGCCCCA TCTGCACAGC 
TACAAGGAGG CCTTTGAAGA GATGGAAATG ACGCCGCCAG TGATCAGCCC GGGGGCCACA 
GTAGCCCGAT CCCCCCCTGG TTTAGCCAAG ACCCCCCTGT CTGCCCTGGG ACTGAAGCCC 
CACAATCCCG CAGAAATCGT TCTCCAGCAA TCGGGAGAGA CAGTCAGGGA AACAGTAATT 
GCTTACATGG AAGAGCCCAG GAGTTATGTT GAATCCGTTG CCCGGACGGC TGCCGCGGGA 
GGAGTCGCAC CCCCATCACC TACAGGCCCC CAGGCATATA GTGTCGATTC TCTGATGAGA 
AATGGGGGTT ACCCCAATGT TTTCACCTCT CCCAGCCCCA TTTCTACCAG CAGCCCCATA 
CACAGCACTG ATGGCTCCCT AAGGAGTTAC CCGTCGGTGG AGAGCGGCGC CCGTGTCCCC 
ACTCCCACTC AGCCTTCCGT GGATTCCAGC TTCAGATCCA CCAGTCTATC GCAGCCGTCG 
CCCCAAAGCA GTTACCAGAA CGCCTCTCCC ATGGTTCCCT ACGCTGGCAC ACCGGCCAGT 
TCCTATGATG GATACCCTTA TTCCCCCAGC AGTGCCCTCA CCCAGACAGA CGGCCTCTCC 
GGGCCACAGA TGAACGCGGT CATTGGTCAC ATGGTTCACG GAAGCCCCAA GGCCGTTCAT 
AGAACGGTGG GGACCAACAC ACCACCGAGT CCAGGTTTTG GGAGGAGAAT GGTCACCCCC 
AATATGGCGC CTCCACCCGG AAGTCCAAGC ATGGGCAGAC ACCCCATGGC TACACATATG 
GCTGCACCCG TTACAGTAGG TAGCCCCAGC CTTCCTCTCC ACCAGGCCTT GATGACGGGA 
AATGCTCCGG TGACTCCTGG TAGCCCCGGC TTGGAGAGAC ACGTTATGTA TGGCTATTCC 
CCCGCTGATG ACAAACGACC GACACTGTCA CGGCAGAGCA GTGCGTCCGG ATACCAGGCA 
CCGCCCACCC CATCCTTCCC GGTCTCCCCG GCCTATTACC ATGGCATGAG TAGCCCTTCC 
TCCTCCTCTC CTGACTCAAC CTTATACCGG CAAGGGAGCC CAATCTCACA GCCGTCTCTG 
CCCGAGAAGA GGAGAATGTC ATCTGGGGAC AGATCCAACA GCCTCCCCAA CTACTCCACC 
CTCAATGGAA AAACCTCTTC GCCCATGTCC AGCGGGATGT CCAGCCCCAG CGGCGGAAGC 
ACAATGGCAT TTACTCACAC TCTACCGGAC TTCTCCAAGT TCTCCATGCC AGACAGCAGC 
CCGGAGACCC GAGCAAATGT GAAGTTTGTG CAGGACACGT CCAAGTACTG GTACAAACCA 
GACATTTCCA GGGATCAGGC CATCTCCCTG CTGAAGGACA AAGACCCCGG GGCGTTCATC 
ATACGAGACA GCCATTCGTT CCGAGGGGCC TACGGACTTG CCATGAAAGT TGCCACCCCG 
CCCCCCAACG CTGGCCAGCC CAGCAAGAAG GGAGATGTGA CCAATGAGCT GGTTCGGCAC 
TTTTTGATTG AGACGAGTCC CAGGGGGGTG AAATTAAAGG GCTGCCAGAA CGAACCCTAC 
TTTGGCTGCC TGTCGGCTCT GGTGTACCAG CACTCCATTA CTCCCCTTGC ACTGCCCTGC 
AAACTGGTCA TACCCTGCAG AGATCCCTCT GATGAGACCA AGGAGACGGC TGTTCCGGCC 
AATTCAGCCT CAGATTTACT GAAACAGGGC GCAGCCTGCA ACGTGTTATT CATAAACTCG 
ATAGACATGG AGTCGCTGAC CGGCCCACAA GCCATTGCGA AGACCATCGC CGAGTCTCTC 
GCCGCCGACC CCCCGCCGGC CGCCGCCATC GTTCACTTCA AAGTCTCCGC CCAAGGAATC 
ACCTTAACCG ACAACCAGAG AAAATTATTT TTCAGACGAC ATTATCCGCT CAGCTCAGTC 
ACCTTCTGCG ACCTGGACCC GCAAGAGAGA AAGTGGATGA AGGCCGATGG GACACAAGCC 
AAACTCTTTG GCTTTGTGGC CAGAAAGCAA GGAAGCACCA CAGACAATAT GTGCCACCTG 
TTTGCCGAAC TTGACCCAAA CCAACCAGCC ACTGCCATCG TCAACTTTGT GTCCCGAGTC 
ATCGGCCTGC AGAAGAGATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031749629.1
CDS	249..5849
Translation	MGCTVSFICCEEDPPLMSAEPQHEAETKEEKKRLLPKPEDLEGQNLHTFKVKTFKKVKSCSVCKQAITREGSACRVCRLSCHRKCEAKIASPCTPRVNYELPSSSESPQKNVETTGSTKSSRSTQSRRRPSRSISLIHAMEDSCELDLTYITERIISVTFSTGTEEPTFCRNLKEVAHMLKSKHGDNYLIFNLSERRHDISKLHSKVLDFGWPDLHAPALEKVCSICKAMDTWLNADPHNVVVIHNKGNRGRTGVVIAAYMHYSNISASADQALDRFAMRRFYEDKVLPVAQPSQKRYINYFSGLLSGAMKMNSKPLFLHHVIMHGIPNFESKGGCRPFLKIYQAMQPVYTSGIYNVQGDSQTSICITIEPGLLLKGDILLKCYHKKFRSPTRDVIFRVQFHTCAIHDLGIVFGKEDLDEAFKDERFPEYGKVEFVFSYGPEKIQGMEHLENGPSVSVDYNTSDPLIRWDSYDNFNVHREDSLEEVGHTQGPLDGSLYAKVKKKDSTNGSTSTVNASGIPLSAAPNHIEHTLSVSSDSGNSTASTKTDKTDEQVATAAANQGLTPEEKQELERLLSGFGLETEGQMHNLNPGTSAAAAMLHVVPAQVHVNGERTSPQVERETDILDDDLPNHDVHSVGSLGTLSSCDVIPSLSETGYQDSHLRMSPLTNGPATVNGIDNLQKHGFIGKETMINGGYPYNNQNVLKSALIPHSQDAGIHMRPSVSAHENLAYQVNQPNWGQPSPSNVTQKHLYYYDPNGMYRSQSYTAAEPGKFENISQLPQAPARSVSSREAVQRGLNSWQQSGSRPPSRDGTTENQVASPSPQPSTFQTVPQSHSIPEFPKMASQKEIEQSIEALNMLMLDLDPSFSQMHKSQSVPVAPREERVLPVQGSFSAQPSLGIYSQPTPQVATQRPSNAYVAPSMVQSAGASQSQPHLPSEQVSRTYSPGVHHDQARAVGPEKVLNDYREPYAAGTFSPYGYQNQQPPLALTRSYSYGSSASPSSVPPLSAYMQSNTQQPVLSSPASSVVMAQPQPQSQSPTREPDDEQYSLEGLVAHRIAEYNNKLRNIRKSMNSPHPHGVQRQRSFTFSGVHTRDKTPDEIGSLARRRTTSEGQYDEGNADLGRASSPTKVRSPVRCVSPEVAFTIAANPGGRPKEPHLHSYKEAFEEMEMTPPVISPGATVARSPPGLAKTPLSALGLKPHNPAEIVLQQSGETVRETVIAYMEEPRSYVESVARTAAAGGVAPPSPTGPQAYSVDSLMRNGGYPNVFTSPSPISTSSPIHSTDGSLRSYPSVESGARVPTPTQPSVDSSFRSTSLSQPSPQSSYQNASPMVPYAGTPASSYDGYPYSPSSALTQTDGLSGPQMNAVIGHMVHGSPKAVHRTVGTNTPPSPGFGRRMVTPNMAPPPGSPSMGRHPMATHMAAPVTVGSPSLPLHQALMTGNAPVTPGSPGLERHVMYGYSPADDKRPTLSRQSSASGYQAPPTPSFPVSPAYYHGMSSPSSSSPDSTLYRQGSPISQPSLPEKRRMSSGDRSNSLPNYSTLNGKTSSPMSSGMSSPSGGSTMAFTHTLPDFSKFSMPDSSPETRANVKFVQDTSKYWYKPDISRDQAISLLKDKDPGAFIIRDSHSFRGAYGLAMKVATPPPNAGQPSKKGDVTNELVRHFLIETSPRGVKLKGCQNEPYFGCLSALVYQHSITPLALPCKLVIPCRDPSDETKETAVPANSASDLLKQGAACNVLFINSIDMESLTGPQAIAKTIAESLAADPPPAAAIVHFKVSAQGITLTDNQRKLFFRRHYPLSSVTFCDLDPQERKWMKADGTQAKLFGFVARKQGSTTDNMCHLFAELDPNQPATAIVNFVSRVIGLQKR

Target ORF information:

RefSeq Version	XM_031893769.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis tensin 1 (tns1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031893769.1

ORF Insert Sequence:

ATGGGATGCA CCGTCAGCTT CATCTGCTGT GAAGAGGACC CCCCGCTCAT GTCTGCTGAG 
CCCCAACATG AGGCCGAAAC GAAGGAGGAA AAAAAGAGGC TCCTTCCCAA GCCTGAAGAC 
CTGGAAGGGC AGAACCTTCA TACATTCAAG GTGAAGACCT TTAAGAAGGT GAAATCGTGC 
AGCGTGTGCA AGCAGGCCAT CACCAGAGAG GGCAGTGCGT GCCGGGTTTG CCGATTGTCG 
TGCCACAGAA AATGTGAAGC CAAGATTGCG TCGCCCTGTA CTCCTCGGGT CAATTATGAG 
CTGCCGTCAA GCTCTGAAAG CCCCCAGAAG AATGTAGAAA CCACAGGATC CACCAAATCC 
TCCAGAAGCA CTCAGTCCCG CCGCAGACCA TCAAGGAGCA TTAGCCTGAT TCACGCCATG 
GAGGACAGCT GCGAGTTGGA CCTCACCTAC ATCACCGAGC GGATCATCTC CGTCACCTTC 
TCCACCGGGA CGGAAGAGCC CACCTTCTGC CGGAACCTGA AGGAAGTGGC GCATATGCTG 
AAGTCCAAAC ATGGAGACAA TTACCTGATC TTCAACTTGT CGGAACGCAG ACATGACATC 
AGCAAGCTCC ATTCCAAGGT GTTGGATTTC GGATGGCCGG ACCTGCACGC GCCGGCCTTG 
GAAAAAGTCT GCAGCATCTG CAAAGCCATG GACACCTGGC TCAACGCCGA CCCTCACAAC 
GTCGTCGTCA TCCATAACAA AGGCAACCGA GGGCGGACAG GTGTGGTGAT TGCCGCCTAT 
ATGCACTACA GCAACATATC TGCCAGTGCA GACCAAGCCT TGGACAGGTT TGCCATGAGA 
AGGTTCTATG AAGACAAAGT CCTTCCAGTA GCTCAACCTT CCCAAAAAAG GTATATAAAC 
TACTTCAGTG GGCTTCTCTC TGGCGCCATG AAAATGAACA GCAAACCTCT CTTCCTGCAT 
CATGTGATCA TGCATGGGAT CCCCAACTTT GAGTCCAAGG GAGGTTGTCG GCCATTTCTG 
AAGATCTACC AAGCGATGCA GCCTGTATAC ACCTCGGGCA TCTACAACGT GCAAGGGGAC 
AGCCAGACGA GCATCTGCAT CACCATAGAA CCGGGGCTGT TACTAAAAGG TGACATCTTG 
CTCAAGTGCT ACCACAAGAA GTTCCGCAGC CCCACGCGGG ACGTCATATT CCGGGTGCAG 
TTCCATACGT GCGCCATCCA TGATCTGGGA ATCGTGTTTG GGAAGGAGGA TCTGGACGAA 
GCTTTCAAAG ATGAGCGTTT TCCCGAATAT GGAAAAGTGG AATTTGTTTT TTCATATGGA 
CCAGAAAAGA TACAAGGCAT GGAGCACCTG GAGAATGGAC CCAGCGTCTC TGTGGATTAT 
AACACATCCG ACCCTCTGAT TCGCTGGGAC TCCTATGATA ACTTCAACGT GCACCGGGAA 
GACAGCCTGG AAGAGGTTGG ACACACTCAA GGCCCCTTGG ATGGAAGCCT GTACGCCAAG 
GTGAAGAAGA AAGACTCTAC GAACGGCAGC ACCAGCACCG TCAACGCCAG CGGAATTCCG 
TTGTCTGCCG CGCCCAATCA CATTGAACAC ACCCTGTCCG TGAGCAGCGA CTCCGGCAAC 
TCGACTGCTT CGACCAAAAC CGACAAGACC GATGAGCAAG TGGCCACGGC GGCTGCCAAC 
CAAGGGTTGA CCCCTGAGGA GAAGCAAGAG TTGGAGCGGC TCCTGAGCGG CTTTGGCTTG 
GAGACCGAAG GGCAGATGCA CAATTTAAAT CCGGGCACAT CTGCAGCAGC AGCGATGCTT 
CACGTTGTGC CGGCGCAAGT CCACGTCAAT GGGGAACGGA CATCTCCGCA GGTGGAACGA 
GAGACAGATA TTCTTGATGA TGATCTGCCC AACCATGATG TTCACAGTGT TGGAAGCTTG 
GGGACCCTGT CTTCGTGTGA TGTGATTCCA AGCTTGAGTG AGACTGGCTA CCAGGATTCT 
CACCTTCGCA TGTCCCCCTT GACCAATGGT CCAGCCACTG TTAATGGCAT TGATAACCTG 
CAAAAACATG GGTTTATTGG TAAAGAGACG ATGATTAATG GCGGCTACCC GTACAACAAC 
CAGAATGTCT TAAAAAGCGC TTTGATCCCT CATTCCCAGG ATGCAGGGAT CCATATGCGA 
CCTTCCGTGT CTGCTCATGA AAACTTAGCC TATCAAGTCA ACCAGCCCAA CTGGGGACAA 
CCTTCTCCAT CAAATGTAAC ACAAAAGCAC TTATACTATT ATGACCCGAA TGGCATGTAC 
CGTTCACAGT CGTACACCGC TGCCGAACCA GGGAAATTCG AAAACATTTC TCAACTACCC 
CAAGCTCCAG CCCGTAGCGT TAGTAGCAGA GAGGCGGTGC AGAGGGGCCT TAACTCGTGG 
CAGCAAAGTG GAAGCAGGCC CCCATCCCGG GATGGAACTA CTGAGAATCA AGTTGCCAGT 
CCTAGCCCGC AGCCAAGCAC TTTCCAGACT GTTCCACAGA GCCACAGCAT CCCAGAATTC 
CCCAAAATGG CTTCCCAGAA GGAGATTGAG CAGTCTATAG AAGCACTTAA TATGCTTATG 
CTGGACCTGG ACCCTTCATT CTCACAAATG CACAAGTCTC AGAGTGTGCC TGTTGCCCCT 
AGAGAAGAAA GGGTTCTGCC AGTGCAAGGA TCCTTCTCAG CGCAGCCATC TTTGGGCATT 
TATAGCCAGC CGACTCCACA GGTGGCTACA CAAAGGCCTA GCAACGCTTA CGTGGCTCCT 
AGCATGGTGC AGAGCGCTGG AGCCTCTCAA AGCCAACCAC ACTTGCCTTC GGAACAGGTT 
TCGAGAACAT ATTCACCTGG GGTGCATCAT GACCAAGCTC GGGCTGTTGG GCCAGAAAAA 
GTTCTGAATG ACTATCGGGA GCCTTATGCT GCTGGAACCT TCTCTCCTTA TGGTTATCAG 
AACCAACAAC CGCCTTTGGC ATTGACAAGA AGCTATAGCT ATGGAAGTAG TGCTTCGCCA 
TCTTCGGTTC CACCATTATC GGCATATATG CAGAGCAATA CTCAGCAACC CGTCCTTTCT 
TCTCCTGCTT CTTCTGTGGT CATGGCTCAA CCTCAGCCTC AATCCCAGTC TCCGACCAGA 
GAGCCAGACG ATGAACAGTA CAGTCTGGAA GGGCTGGTAG CTCACAGAAT TGCTGAGTAC 
AACAACAAAC TCCGGAATAT CCGTAAAAGC ATGAATAGCC CCCATCCCCA TGGCGTGCAG 
CGCCAGCGTT CCTTCACTTT CTCTGGGGTG CACACTCGGG ACAAGACTCC GGATGAGATC 
GGGTCCCTGG CACGGAGGCG CACCACCAGC GAGGGGCAAT ATGACGAGGG CAATGCTGAC 
CTGGGCAGAG CGAGTAGCCC CACCAAGGTG CGCTCCCCAG TGCGCTGCGT CTCCCCGGAA 
GTCGCCTTCA CCATTGCGGC AAACCCAGGG GGGAGACCCA AGGAGCCCCA TCTGCACAGC 
TACAAGGAGG CCTTTGAAGA GATGGAAATG ACGCCGCCAG TGATCAGCCC GGGGGCCACA 
GTAGCCCGAT CCCCCCCTGG TTTAGCCAAG ACCCCCCTGT CTGCCCTGGG ACTGAAGCCC 
CACAATCCCG CAGAAATCGT TCTCCAGCAA TCGGGAGAGA CAGTCAGGGA AACAGTAATT 
GCTTACATGG AAGAGCCCAG GAGTTATGTT GAATCCGTTG CCCGGACGGC TGCCGCGGGA 
GGAGTCGCAC CCCCATCACC TACAGGCCCC CAGGCATATA GTGTCGATTC TCTGATGAGA 
AATGGGGGTT ACCCCAATGT TTTCACCTCT CCCAGCCCCA TTTCTACCAG CAGCCCCATA 
CACAGCACTG ATGGCTCCCT AAGGAGTTAC CCGTCGGTGG AGAGCGGCGC CCGTGTCCCC 
ACTCCCACTC AGCCTTCCGT GGATTCCAGC TTCAGATCCA CCAGTCTATC GCAGCCGTCG 
CCCCAAAGCA GTTACCAGAA CGCCTCTCCC ATGGTTCCCT ACGCTGGCAC ACCGGCCAGT 
TCCTATGATG GATACCCTTA TTCCCCCAGC AGTGCCCTCA CCCAGACAGA CGGCCTCTCC 
GGGCCACAGA TGAACGCGGT CATTGGTCAC ATGGTTCACG GAAGCCCCAA GGCCGTTCAT 
AGAACGGTGG GGACCAACAC ACCACCGAGT CCAGGTTTTG GGAGGAGAAT GGTCACCCCC 
AATATGGCGC CTCCACCCGG AAGTCCAAGC ATGGGCAGAC ACCCCATGGC TACACATATG 
GCTGCACCCG TTACAGTAGG TAGCCCCAGC CTTCCTCTCC ACCAGGCCTT GATGACGGGA 
AATGCTCCGG TGACTCCTGG TAGCCCCGGC TTGGAGAGAC ACGTTATGTA TGGCTATTCC 
CCCGCTGATG ACAAACGACC GACACTGTCA CGGCAGAGCA GTGCGTCCGG ATACCAGGCA 
CCGCCCACCC CATCCTTCCC GGTCTCCCCG GCCTATTACC ATGGCATGAG TAGCCCTTCC 
TCCTCCTCTC CTGACTCAAC CTTATACCGG CAAGGGAGCC CAATCTCACA GCCGTCTCTG 
CCCGAGAAGA GGAGAATGTC ATCTGGGGAC AGATCCAACA GCCTCCCCAA CTACTCCACC 
CTCAATGGAA AAACCTCTTC GCCCATGTCC AGCGGGATGT CCAGCCCCAG CGGCGGAAGC 
ACAATGGCAT TTACTCACAC TCTACCGGAC TTCTCCAAGT TCTCCATGCC AGACAGCAGC 
CCGGAGACCC GAGCAAATGT GAAGTTTGTG CAGGACACGT CCAAGTACTG GTACAAACCA 
GACATTTCCA GGGATCAGGC CATCTCCCTG CTGAAGGACA AAGACCCCGG GGCGTTCATC 
ATACGAGACA GCCATTCGTT CCGAGGGGCC TACGGACTTG CCATGAAAGT TGCCACCCCG 
CCCCCCAACG CTGGCCAGCC CAGCAAGAAG GGAGATGTGA CCAATGAGCT GGTTCGGCAC 
TTTTTGATTG AGACGAGTCC CAGGGGGGTG AAATTAAAGG GCTGCCAGAA CGAACCCTAC 
TTTGGCTGCC TGTCGGCTCT GGTGTACCAG CACTCCATTA CTCCCCTTGC ACTGCCCTGC 
AAACTGGTCA TACCCTGCAG AGATCCCTCT GATGAGACCA AGGAGACGGC TGTTCCGGCC 
AATTCAGCCT CAGATTTACT GAAACAGGGC GCAGCCTGCA ACGTGTTATT CATAAACTCG 
ATAGACATGG AGTCGCTGAC CGGCCCACAA GCCATTGCGA AGACCATCGC CGAGTCTCTC 
GCCGCCGACC CCCCGCCGGC CGCCGCCATC GTTCACTTCA AAGTCTCCGC CCAAGGAATC 
ACCTTAACCG ACAACCAGAG AAAATTATTT TTCAGACGAC ATTATCCGCT CAGCTCAGTC 
ACCTTCTGCG ACCTGGACCC GCAAGAGAGA AAGTGGATGA AGGCCGATGG GACACAAGCC 
AAACTCTTTG GCTTTGTGGC CAGAAAGCAA GGAAGCACCA CAGACAATAT GTGCCACCTG 
TTTGCCGAAC TTGACCCAAA CCAACCAGCC ACTGCCATCG TCAACTTTGT GTCCCGAGTC 
ATCGGCCTGC AGAAGAGATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

tns1 ( XM_031893770.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_031749630.1 Xenopus tropicalis tensin 1 (tns1), transcript variant X2, mRNA.

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CloneID	OXa67402
Clone ID Related Accession (Same CDS sequence)	XM_031893770.1
Accession Version	XM_031893770.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5571bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1576512000000
Organism	Xenopus tropicalis(tropical clawed frog)
Product	tensin-1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030685.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCCTCTG CATCTCACTG CCTCCCCAGA ACTGCTTCGG ACCGCAGGAT GACCTGGCTG 
TGCCTGTCCT GTATGCTCTG GCCTGAAGAC CTGGAAGGGC AGAACCTTCA TACATTCAAG 
GTGAAGACCT TTAAGAAGGT GAAATCGTGC AGCGTGTGCA AGCAGGCCAT CACCAGAGAG 
GGCAGTGCGT GCCGGGTTTG CCGATTGTCG TGCCACAGAA AATGTGAAGC CAAGATTGCG 
TCGCCCTGTA CTCCTCGGGT CAATTATGAG CTGCCGTCAA GCTCTGAAAG CCCCCAGAAG 
AATGTAGAAA CCACAGGATC CACCAAATCC TCCAGAAGCA CTCAGTCCCG CCGCAGACCA 
TCAAGGAGCA TTAGCCTGAT TCACGCCATG GAGGACAGCT GCGAGTTGGA CCTCACCTAC 
ATCACCGAGC GGATCATCTC CGTCACCTTC TCCACCGGGA CGGAAGAGCC CACCTTCTGC 
CGGAACCTGA AGGAAGTGGC GCATATGCTG AAGTCCAAAC ATGGAGACAA TTACCTGATC 
TTCAACTTGT CGGAACGCAG ACATGACATC AGCAAGCTCC ATTCCAAGGT GTTGGATTTC 
GGATGGCCGG ACCTGCACGC GCCGGCCTTG GAAAAAGTCT GCAGCATCTG CAAAGCCATG 
GACACCTGGC TCAACGCCGA CCCTCACAAC GTCGTCGTCA TCCATAACAA AGGCAACCGA 
GGGCGGACAG GTGTGGTGAT TGCCGCCTAT ATGCACTACA GCAACATATC TGCCAGTGCA 
GACCAAGCCT TGGACAGGTT TGCCATGAGA AGGTTCTATG AAGACAAAGT CCTTCCAGTA 
GCTCAACCTT CCCAAAAAAG GTATATAAAC TACTTCAGTG GGCTTCTCTC TGGCGCCATG 
AAAATGAACA GCAAACCTCT CTTCCTGCAT CATGTGATCA TGCATGGGAT CCCCAACTTT 
GAGTCCAAGG GAGGTTGTCG GCCATTTCTG AAGATCTACC AAGCGATGCA GCCTGTATAC 
ACCTCGGGCA TCTACAACGT GCAAGGGGAC AGCCAGACGA GCATCTGCAT CACCATAGAA 
CCGGGGCTGT TACTAAAAGG TGACATCTTG CTCAAGTGCT ACCACAAGAA GTTCCGCAGC 
CCCACGCGGG ACGTCATATT CCGGGTGCAG TTCCATACGT GCGCCATCCA TGATCTGGGA 
ATCGTGTTTG GGAAGGAGGA TCTGGACGAA GCTTTCAAAG ATGAGCGTTT TCCCGAATAT 
GGAAAAGTGG AATTTGTTTT TTCATATGGA CCAGAAAAGA TACAAGGCAT GGAGCACCTG 
GAGAATGGAC CCAGCGTCTC TGTGGATTAT AACACATCCG ACCCTCTGAT TCGCTGGGAC 
TCCTATGATA ACTTCAACGT GCACCGGGAA GACAGCCTGG AAGAGGTTGG ACACACTCAA 
GGCCCCTTGG ATGGAAGCCT GTACGCCAAG GTGAAGAAGA AAGACTCTAC GAACGGCAGC 
ACCAGCACCG TCAACGCCAG CGGAATTCCG TTGTCTGCCG CGCCCAATCA CATTGAACAC 
ACCCTGTCCG TGAGCAGCGA CTCCGGCAAC TCGACTGCTT CGACCAAAAC CGACAAGACC 
GATGAGCAAG TGGCCACGGC GGCTGCCAAC CAAGGGTTGA CCCCTGAGGA GAAGCAAGAG 
TTGGAGCGGC TCCTGAGCGG CTTTGGCTTG GAGACCGAAG GGCAGATGCA CAATTTAAAT 
CCGGGCACAT CTGCAGCAGC AGCGATGCTT CACGTTGTGC CGGCGCAAGT CCACGTCAAT 
GGGGAACGGA CATCTCCGCA GGTGGAACGA GAGACAGATA TTCTTGATGA TGATCTGCCC 
AACCATGATG TTCACAGTGT TGGAAGCTTG GGGACCCTGT CTTCGTGTGA TGTGATTCCA 
AGCTTGAGTG AGACTGGCTA CCAGGATTCT CACCTTCGCA TGTCCCCCTT GACCAATGGT 
CCAGCCACTG TTAATGGCAT TGATAACCTG CAAAAACATG GGTTTATTGG TAAAGAGACG 
ATGATTAATG GCGGCTACCC GTACAACAAC CAGAATGTCT TAAAAAGCGC TTTGATCCCT 
CATTCCCAGG ATGCAGGGAT CCATATGCGA CCTTCCGTGT CTGCTCATGA AAACTTAGCC 
TATCAAGTCA ACCAGCCCAA CTGGGGACAA CCTTCTCCAT CAAATGTAAC ACAAAAGCAC 
TTATACTATT ATGACCCGAA TGGCATGTAC CGTTCACAGT CGTACACCGC TGCCGAACCA 
GGGAAATTCG AAAACATTTC TCAACTACCC CAAGCTCCAG CCCGTAGCGT TAGTAGCAGA 
GAGGCGGTGC AGAGGGGCCT TAACTCGTGG CAGCAAAGTG GAAGCAGGCC CCCATCCCGG 
GATGGAACTA CTGAGAATCA AGTTGCCAGT CCTAGCCCGC AGCCAAGCAC TTTCCAGACT 
GTTCCACAGA GCCACAGCAT CCCAGAATTC CCCAAAATGG CTTCCCAGAA GGAGATTGAG 
CAGTCTATAG AAGCACTTAA TATGCTTATG CTGGACCTGG ACCCTTCATT CTCACAAATG 
CACAAGTCTC AGAGTGTGCC TGTTGCCCCT AGAGAAGAAA GGGTTCTGCC AGTGCAAGGA 
TCCTTCTCAG CGCAGCCATC TTTGGGCATT TATAGCCAGC CGACTCCACA GGTGGCTACA 
CAAAGGCCTA GCAACGCTTA CGTGGCTCCT AGCATGGTGC AGAGCGCTGG AGCCTCTCAA 
AGCCAACCAC ACTTGCCTTC GGAACAGGTT TCGAGAACAT ATTCACCTGG GGTGCATCAT 
GACCAAGCTC GGGCTGTTGG GCCAGAAAAA GTTCTGAATG ACTATCGGGA GCCTTATGCT 
GCTGGAACCT TCTCTCCTTA TGGTTATCAG AACCAACAAC CGCCTTTGGC ATTGACAAGA 
AGCTATAGCT ATGGAAGTAG TGCTTCGCCA TCTTCGGTTC CACCATTATC GGCATATATG 
CAGAGCAATA CTCAGCAACC CGTCCTTTCT TCTCCTGCTT CTTCTGTGGT CATGGCTCAA 
CCTCAGCCTC AATCCCAGTC TCCGACCAGA GAGCCAGACG ATGAACAGTA CAGTCTGGAA 
GGGCTGGTAG CTCACAGAAT TGCTGAGTAC AACAACAAAC TCCGGAATAT CCGTAAAAGC 
ATGAATAGCC CCCATCCCCA TGGCGTGCAG CGCCAGCGTT CCTTCACTTT CTCTGGGGTG 
CACACTCGGG ACAAGACTCC GGATGAGATC GGGTCCCTGG CACGGAGGCG CACCACCAGC 
GAGGGGCAAT ATGACGAGGG CAATGCTGAC CTGGGCAGAG CGAGTAGCCC CACCAAGGTG 
CGCTCCCCAG TGCGCTGCGT CTCCCCGGAA GTCGCCTTCA CCATTGCGGC AAACCCAGGG 
GGGAGACCCA AGGAGCCCCA TCTGCACAGC TACAAGGAGG CCTTTGAAGA GATGGAAATG 
ACGCCGCCAG TGATCAGCCC GGGGGCCACA GTAGCCCGAT CCCCCCCTGG TTTAGCCAAG 
ACCCCCCTGT CTGCCCTGGG ACTGAAGCCC CACAATCCCG CAGAAATCGT TCTCCAGCAA 
TCGGGAGAGA CAGTCAGGGA AACAGTAATT GCTTACATGG AAGAGCCCAG GAGTTATGTT 
GAATCCGTTG CCCGGACGGC TGCCGCGGGA GGAGTCGCAC CCCCATCACC TACAGGCCCC 
CAGGCATATA GTGTCGATTC TCTGATGAGA AATGGGGGTT ACCCCAATGT TTTCACCTCT 
CCCAGCCCCA TTTCTACCAG CAGCCCCATA CACAGCACTG ATGGCTCCCT AAGGAGTTAC 
CCGTCGGTGG AGAGCGGCGC CCGTGTCCCC ACTCCCACTC AGCCTTCCGT GGATTCCAGC 
TTCAGATCCA CCAGTCTATC GCAGCCGTCG CCCCAAAGCA GTTACCAGAA CGCCTCTCCC 
ATGGTTCCCT ACGCTGGCAC ACCGGCCAGT TCCTATGATG GATACCCTTA TTCCCCCAGC 
AGTGCCCTCA CCCAGACAGA CGGCCTCTCC GGGCCACAGA TGAACGCGGT CATTGGTCAC 
ATGGTTCACG GAAGCCCCAA GGCCGTTCAT AGAACGGTGG GGACCAACAC ACCACCGAGT 
CCAGGTTTTG GGAGGAGAAT GGTCACCCCC AATATGGCGC CTCCACCCGG AAGTCCAAGC 
ATGGGCAGAC ACCCCATGGC TACACATATG GCTGCACCCG TTACAGTAGG TAGCCCCAGC 
CTTCCTCTCC ACCAGGCCTT GATGACGGGA AATGCTCCGG TGACTCCTGG TAGCCCCGGC 
TTGGAGAGAC ACGTTATGTA TGGCTATTCC CCCGCTGATG ACAAACGACC GACACTGTCA 
CGGCAGAGCA GTGCGTCCGG ATACCAGGCA CCGCCCACCC CATCCTTCCC GGTCTCCCCG 
GCCTATTACC ATGGCATGAG TAGCCCTTCC TCCTCCTCTC CTGACTCAAC CTTATACCGG 
CAAGGGAGCC CAATCTCACA GCCGTCTCTG CCCGAGAAGA GGAGAATGTC ATCTGGGGAC 
AGATCCAACA GCCTCCCCAA CTACTCCACC CTCAATGGAA AAACCTCTTC GCCCATGTCC 
AGCGGGATGT CCAGCCCCAG CGGCGGAAGC ACAATGGCAT TTACTCACAC TCTACCGGAC 
TTCTCCAAGT TCTCCATGCC AGACAGCAGC CCGGAGACCC GAGCAAATGT GAAGTTTGTG 
CAGGACACGT CCAAGTACTG GTACAAACCA GACATTTCCA GGGATCAGGC CATCTCCCTG 
CTGAAGGACA AAGACCCCGG GGCGTTCATC ATACGAGACA GCCATTCGTT CCGAGGGGCC 
TACGGACTTG CCATGAAAGT TGCCACCCCG CCCCCCAACG CTGGCCAGCC CAGCAAGAAG 
GGAGATGTGA CCAATGAGCT GGTTCGGCAC TTTTTGATTG AGACGAGTCC CAGGGGGGTG 
AAATTAAAGG GCTGCCAGAA CGAACCCTAC TTTGGCTGCC TGTCGGCTCT GGTGTACCAG 
CACTCCATTA CTCCCCTTGC ACTGCCCTGC AAACTGGTCA TACCCTGCAG AGATCCCTCT 
GATGAGACCA AGGAGACGGC TGTTCCGGCC AATTCAGCCT CAGATTTACT GAAACAGGGC 
GCAGCCTGCA ACGTGTTATT CATAAACTCG ATAGACATGG AGTCGCTGAC CGGCCCACAA 
GCCATTGCGA AGACCATCGC CGAGTCTCTC GCCGCCGACC CCCCGCCGGC CGCCGCCATC 
GTTCACTTCA AAGTCTCCGC CCAAGGAATC ACCTTAACCG ACAACCAGAG AAAATTATTT 
TTCAGACGAC ATTATCCGCT CAGCTCAGTC ACCTTCTGCG ACCTGGACCC GCAAGAGAGA 
AAGTGGATGA AGGCCGATGG GACACAAGCC AAACTCTTTG GCTTTGTGGC CAGAAAGCAA 
GGAAGCACCA CAGACAATAT GTGCCACCTG TTTGCCGAAC TTGACCCAAA CCAACCAGCC 
ACTGCCATCG TCAACTTTGT GTCCCGAGTC ATCGGCCTGC AGAAGAGATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031749630.1
CDS	422..5992
Translation	MPSASHCLPRTASDRRMTWLCLSCMLWPEDLEGQNLHTFKVKTFKKVKSCSVCKQAITREGSACRVCRLSCHRKCEAKIASPCTPRVNYELPSSSESPQKNVETTGSTKSSRSTQSRRRPSRSISLIHAMEDSCELDLTYITERIISVTFSTGTEEPTFCRNLKEVAHMLKSKHGDNYLIFNLSERRHDISKLHSKVLDFGWPDLHAPALEKVCSICKAMDTWLNADPHNVVVIHNKGNRGRTGVVIAAYMHYSNISASADQALDRFAMRRFYEDKVLPVAQPSQKRYINYFSGLLSGAMKMNSKPLFLHHVIMHGIPNFESKGGCRPFLKIYQAMQPVYTSGIYNVQGDSQTSICITIEPGLLLKGDILLKCYHKKFRSPTRDVIFRVQFHTCAIHDLGIVFGKEDLDEAFKDERFPEYGKVEFVFSYGPEKIQGMEHLENGPSVSVDYNTSDPLIRWDSYDNFNVHREDSLEEVGHTQGPLDGSLYAKVKKKDSTNGSTSTVNASGIPLSAAPNHIEHTLSVSSDSGNSTASTKTDKTDEQVATAAANQGLTPEEKQELERLLSGFGLETEGQMHNLNPGTSAAAAMLHVVPAQVHVNGERTSPQVERETDILDDDLPNHDVHSVGSLGTLSSCDVIPSLSETGYQDSHLRMSPLTNGPATVNGIDNLQKHGFIGKETMINGGYPYNNQNVLKSALIPHSQDAGIHMRPSVSAHENLAYQVNQPNWGQPSPSNVTQKHLYYYDPNGMYRSQSYTAAEPGKFENISQLPQAPARSVSSREAVQRGLNSWQQSGSRPPSRDGTTENQVASPSPQPSTFQTVPQSHSIPEFPKMASQKEIEQSIEALNMLMLDLDPSFSQMHKSQSVPVAPREERVLPVQGSFSAQPSLGIYSQPTPQVATQRPSNAYVAPSMVQSAGASQSQPHLPSEQVSRTYSPGVHHDQARAVGPEKVLNDYREPYAAGTFSPYGYQNQQPPLALTRSYSYGSSASPSSVPPLSAYMQSNTQQPVLSSPASSVVMAQPQPQSQSPTREPDDEQYSLEGLVAHRIAEYNNKLRNIRKSMNSPHPHGVQRQRSFTFSGVHTRDKTPDEIGSLARRRTTSEGQYDEGNADLGRASSPTKVRSPVRCVSPEVAFTIAANPGGRPKEPHLHSYKEAFEEMEMTPPVISPGATVARSPPGLAKTPLSALGLKPHNPAEIVLQQSGETVRETVIAYMEEPRSYVESVARTAAAGGVAPPSPTGPQAYSVDSLMRNGGYPNVFTSPSPISTSSPIHSTDGSLRSYPSVESGARVPTPTQPSVDSSFRSTSLSQPSPQSSYQNASPMVPYAGTPASSYDGYPYSPSSALTQTDGLSGPQMNAVIGHMVHGSPKAVHRTVGTNTPPSPGFGRRMVTPNMAPPPGSPSMGRHPMATHMAAPVTVGSPSLPLHQALMTGNAPVTPGSPGLERHVMYGYSPADDKRPTLSRQSSASGYQAPPTPSFPVSPAYYHGMSSPSSSSPDSTLYRQGSPISQPSLPEKRRMSSGDRSNSLPNYSTLNGKTSSPMSSGMSSPSGGSTMAFTHTLPDFSKFSMPDSSPETRANVKFVQDTSKYWYKPDISRDQAISLLKDKDPGAFIIRDSHSFRGAYGLAMKVATPPPNAGQPSKKGDVTNELVRHFLIETSPRGVKLKGCQNEPYFGCLSALVYQHSITPLALPCKLVIPCRDPSDETKETAVPANSASDLLKQGAACNVLFINSIDMESLTGPQAIAKTIAESLAADPPPAAAIVHFKVSAQGITLTDNQRKLFFRRHYPLSSVTFCDLDPQERKWMKADGTQAKLFGFVARKQGSTTDNMCHLFAELDPNQPATAIVNFVSRVIGLQKR

Target ORF information:

RefSeq Version	XM_031893770.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis tensin 1 (tns1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031893770.1

ORF Insert Sequence:

ATGCCCTCTG CATCTCACTG CCTCCCCAGA ACTGCTTCGG ACCGCAGGAT GACCTGGCTG 
TGCCTGTCCT GTATGCTCTG GCCTGAAGAC CTGGAAGGGC AGAACCTTCA TACATTCAAG 
GTGAAGACCT TTAAGAAGGT GAAATCGTGC AGCGTGTGCA AGCAGGCCAT CACCAGAGAG 
GGCAGTGCGT GCCGGGTTTG CCGATTGTCG TGCCACAGAA AATGTGAAGC CAAGATTGCG 
TCGCCCTGTA CTCCTCGGGT CAATTATGAG CTGCCGTCAA GCTCTGAAAG CCCCCAGAAG 
AATGTAGAAA CCACAGGATC CACCAAATCC TCCAGAAGCA CTCAGTCCCG CCGCAGACCA 
TCAAGGAGCA TTAGCCTGAT TCACGCCATG GAGGACAGCT GCGAGTTGGA CCTCACCTAC 
ATCACCGAGC GGATCATCTC CGTCACCTTC TCCACCGGGA CGGAAGAGCC CACCTTCTGC 
CGGAACCTGA AGGAAGTGGC GCATATGCTG AAGTCCAAAC ATGGAGACAA TTACCTGATC 
TTCAACTTGT CGGAACGCAG ACATGACATC AGCAAGCTCC ATTCCAAGGT GTTGGATTTC 
GGATGGCCGG ACCTGCACGC GCCGGCCTTG GAAAAAGTCT GCAGCATCTG CAAAGCCATG 
GACACCTGGC TCAACGCCGA CCCTCACAAC GTCGTCGTCA TCCATAACAA AGGCAACCGA 
GGGCGGACAG GTGTGGTGAT TGCCGCCTAT ATGCACTACA GCAACATATC TGCCAGTGCA 
GACCAAGCCT TGGACAGGTT TGCCATGAGA AGGTTCTATG AAGACAAAGT CCTTCCAGTA 
GCTCAACCTT CCCAAAAAAG GTATATAAAC TACTTCAGTG GGCTTCTCTC TGGCGCCATG 
AAAATGAACA GCAAACCTCT CTTCCTGCAT CATGTGATCA TGCATGGGAT CCCCAACTTT 
GAGTCCAAGG GAGGTTGTCG GCCATTTCTG AAGATCTACC AAGCGATGCA GCCTGTATAC 
ACCTCGGGCA TCTACAACGT GCAAGGGGAC AGCCAGACGA GCATCTGCAT CACCATAGAA 
CCGGGGCTGT TACTAAAAGG TGACATCTTG CTCAAGTGCT ACCACAAGAA GTTCCGCAGC 
CCCACGCGGG ACGTCATATT CCGGGTGCAG TTCCATACGT GCGCCATCCA TGATCTGGGA 
ATCGTGTTTG GGAAGGAGGA TCTGGACGAA GCTTTCAAAG ATGAGCGTTT TCCCGAATAT 
GGAAAAGTGG AATTTGTTTT TTCATATGGA CCAGAAAAGA TACAAGGCAT GGAGCACCTG 
GAGAATGGAC CCAGCGTCTC TGTGGATTAT AACACATCCG ACCCTCTGAT TCGCTGGGAC 
TCCTATGATA ACTTCAACGT GCACCGGGAA GACAGCCTGG AAGAGGTTGG ACACACTCAA 
GGCCCCTTGG ATGGAAGCCT GTACGCCAAG GTGAAGAAGA AAGACTCTAC GAACGGCAGC 
ACCAGCACCG TCAACGCCAG CGGAATTCCG TTGTCTGCCG CGCCCAATCA CATTGAACAC 
ACCCTGTCCG TGAGCAGCGA CTCCGGCAAC TCGACTGCTT CGACCAAAAC CGACAAGACC 
GATGAGCAAG TGGCCACGGC GGCTGCCAAC CAAGGGTTGA CCCCTGAGGA GAAGCAAGAG 
TTGGAGCGGC TCCTGAGCGG CTTTGGCTTG GAGACCGAAG GGCAGATGCA CAATTTAAAT 
CCGGGCACAT CTGCAGCAGC AGCGATGCTT CACGTTGTGC CGGCGCAAGT CCACGTCAAT 
GGGGAACGGA CATCTCCGCA GGTGGAACGA GAGACAGATA TTCTTGATGA TGATCTGCCC 
AACCATGATG TTCACAGTGT TGGAAGCTTG GGGACCCTGT CTTCGTGTGA TGTGATTCCA 
AGCTTGAGTG AGACTGGCTA CCAGGATTCT CACCTTCGCA TGTCCCCCTT GACCAATGGT 
CCAGCCACTG TTAATGGCAT TGATAACCTG CAAAAACATG GGTTTATTGG TAAAGAGACG 
ATGATTAATG GCGGCTACCC GTACAACAAC CAGAATGTCT TAAAAAGCGC TTTGATCCCT 
CATTCCCAGG ATGCAGGGAT CCATATGCGA CCTTCCGTGT CTGCTCATGA AAACTTAGCC 
TATCAAGTCA ACCAGCCCAA CTGGGGACAA CCTTCTCCAT CAAATGTAAC ACAAAAGCAC 
TTATACTATT ATGACCCGAA TGGCATGTAC CGTTCACAGT CGTACACCGC TGCCGAACCA 
GGGAAATTCG AAAACATTTC TCAACTACCC CAAGCTCCAG CCCGTAGCGT TAGTAGCAGA 
GAGGCGGTGC AGAGGGGCCT TAACTCGTGG CAGCAAAGTG GAAGCAGGCC CCCATCCCGG 
GATGGAACTA CTGAGAATCA AGTTGCCAGT CCTAGCCCGC AGCCAAGCAC TTTCCAGACT 
GTTCCACAGA GCCACAGCAT CCCAGAATTC CCCAAAATGG CTTCCCAGAA GGAGATTGAG 
CAGTCTATAG AAGCACTTAA TATGCTTATG CTGGACCTGG ACCCTTCATT CTCACAAATG 
CACAAGTCTC AGAGTGTGCC TGTTGCCCCT AGAGAAGAAA GGGTTCTGCC AGTGCAAGGA 
TCCTTCTCAG CGCAGCCATC TTTGGGCATT TATAGCCAGC CGACTCCACA GGTGGCTACA 
CAAAGGCCTA GCAACGCTTA CGTGGCTCCT AGCATGGTGC AGAGCGCTGG AGCCTCTCAA 
AGCCAACCAC ACTTGCCTTC GGAACAGGTT TCGAGAACAT ATTCACCTGG GGTGCATCAT 
GACCAAGCTC GGGCTGTTGG GCCAGAAAAA GTTCTGAATG ACTATCGGGA GCCTTATGCT 
GCTGGAACCT TCTCTCCTTA TGGTTATCAG AACCAACAAC CGCCTTTGGC ATTGACAAGA 
AGCTATAGCT ATGGAAGTAG TGCTTCGCCA TCTTCGGTTC CACCATTATC GGCATATATG 
CAGAGCAATA CTCAGCAACC CGTCCTTTCT TCTCCTGCTT CTTCTGTGGT CATGGCTCAA 
CCTCAGCCTC AATCCCAGTC TCCGACCAGA GAGCCAGACG ATGAACAGTA CAGTCTGGAA 
GGGCTGGTAG CTCACAGAAT TGCTGAGTAC AACAACAAAC TCCGGAATAT CCGTAAAAGC 
ATGAATAGCC CCCATCCCCA TGGCGTGCAG CGCCAGCGTT CCTTCACTTT CTCTGGGGTG 
CACACTCGGG ACAAGACTCC GGATGAGATC GGGTCCCTGG CACGGAGGCG CACCACCAGC 
GAGGGGCAAT ATGACGAGGG CAATGCTGAC CTGGGCAGAG CGAGTAGCCC CACCAAGGTG 
CGCTCCCCAG TGCGCTGCGT CTCCCCGGAA GTCGCCTTCA CCATTGCGGC AAACCCAGGG 
GGGAGACCCA AGGAGCCCCA TCTGCACAGC TACAAGGAGG CCTTTGAAGA GATGGAAATG 
ACGCCGCCAG TGATCAGCCC GGGGGCCACA GTAGCCCGAT CCCCCCCTGG TTTAGCCAAG 
ACCCCCCTGT CTGCCCTGGG ACTGAAGCCC CACAATCCCG CAGAAATCGT TCTCCAGCAA 
TCGGGAGAGA CAGTCAGGGA AACAGTAATT GCTTACATGG AAGAGCCCAG GAGTTATGTT 
GAATCCGTTG CCCGGACGGC TGCCGCGGGA GGAGTCGCAC CCCCATCACC TACAGGCCCC 
CAGGCATATA GTGTCGATTC TCTGATGAGA AATGGGGGTT ACCCCAATGT TTTCACCTCT 
CCCAGCCCCA TTTCTACCAG CAGCCCCATA CACAGCACTG ATGGCTCCCT AAGGAGTTAC 
CCGTCGGTGG AGAGCGGCGC CCGTGTCCCC ACTCCCACTC AGCCTTCCGT GGATTCCAGC 
TTCAGATCCA CCAGTCTATC GCAGCCGTCG CCCCAAAGCA GTTACCAGAA CGCCTCTCCC 
ATGGTTCCCT ACGCTGGCAC ACCGGCCAGT TCCTATGATG GATACCCTTA TTCCCCCAGC 
AGTGCCCTCA CCCAGACAGA CGGCCTCTCC GGGCCACAGA TGAACGCGGT CATTGGTCAC 
ATGGTTCACG GAAGCCCCAA GGCCGTTCAT AGAACGGTGG GGACCAACAC ACCACCGAGT 
CCAGGTTTTG GGAGGAGAAT GGTCACCCCC AATATGGCGC CTCCACCCGG AAGTCCAAGC 
ATGGGCAGAC ACCCCATGGC TACACATATG GCTGCACCCG TTACAGTAGG TAGCCCCAGC 
CTTCCTCTCC ACCAGGCCTT GATGACGGGA AATGCTCCGG TGACTCCTGG TAGCCCCGGC 
TTGGAGAGAC ACGTTATGTA TGGCTATTCC CCCGCTGATG ACAAACGACC GACACTGTCA 
CGGCAGAGCA GTGCGTCCGG ATACCAGGCA CCGCCCACCC CATCCTTCCC GGTCTCCCCG 
GCCTATTACC ATGGCATGAG TAGCCCTTCC TCCTCCTCTC CTGACTCAAC CTTATACCGG 
CAAGGGAGCC CAATCTCACA GCCGTCTCTG CCCGAGAAGA GGAGAATGTC ATCTGGGGAC 
AGATCCAACA GCCTCCCCAA CTACTCCACC CTCAATGGAA AAACCTCTTC GCCCATGTCC 
AGCGGGATGT CCAGCCCCAG CGGCGGAAGC ACAATGGCAT TTACTCACAC TCTACCGGAC 
TTCTCCAAGT TCTCCATGCC AGACAGCAGC CCGGAGACCC GAGCAAATGT GAAGTTTGTG 
CAGGACACGT CCAAGTACTG GTACAAACCA GACATTTCCA GGGATCAGGC CATCTCCCTG 
CTGAAGGACA AAGACCCCGG GGCGTTCATC ATACGAGACA GCCATTCGTT CCGAGGGGCC 
TACGGACTTG CCATGAAAGT TGCCACCCCG CCCCCCAACG CTGGCCAGCC CAGCAAGAAG 
GGAGATGTGA CCAATGAGCT GGTTCGGCAC TTTTTGATTG AGACGAGTCC CAGGGGGGTG 
AAATTAAAGG GCTGCCAGAA CGAACCCTAC TTTGGCTGCC TGTCGGCTCT GGTGTACCAG 
CACTCCATTA CTCCCCTTGC ACTGCCCTGC AAACTGGTCA TACCCTGCAG AGATCCCTCT 
GATGAGACCA AGGAGACGGC TGTTCCGGCC AATTCAGCCT CAGATTTACT GAAACAGGGC 
GCAGCCTGCA ACGTGTTATT CATAAACTCG ATAGACATGG AGTCGCTGAC CGGCCCACAA 
GCCATTGCGA AGACCATCGC CGAGTCTCTC GCCGCCGACC CCCCGCCGGC CGCCGCCATC 
GTTCACTTCA AAGTCTCCGC CCAAGGAATC ACCTTAACCG ACAACCAGAG AAAATTATTT 
TTCAGACGAC ATTATCCGCT CAGCTCAGTC ACCTTCTGCG ACCTGGACCC GCAAGAGAGA 
AAGTGGATGA AGGCCGATGG GACACAAGCC AAACTCTTTG GCTTTGTGGC CAGAAAGCAA 
GGAAGCACCA CAGACAATAT GTGCCACCTG TTTGCCGAAC TTGACCCAAA CCAACCAGCC 
ACTGCCATCG TCAACTTTGT GTCCCGAGTC ATCGGCCTGC AGAAGAGATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

tns1 ( XM_031893771.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_031749631.1 Xenopus tropicalis tensin 1 (tns1), transcript variant X3, mRNA.

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CloneID	OXa67403
Clone ID Related Accession (Same CDS sequence)	XM_031893771.1
Accession Version	XM_031893771.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5565bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1576512000000
Organism	Xenopus tropicalis(tropical clawed frog)
Product	tensin-1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030685.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGATGCA CCGTCAGCTT CATCTGCTGT GAAGAGGACC CCCCGCTCAT GTCTGCTGAG 
CCCCAACATG AGGCCGAAAC GAAGGAGGAA AAAAAGAGGC TCCTTCCCAA GCCTGAAGAC 
CTGGAAGGGC AGAACCTTCA TACATTCAAG GTGAAGACCT TTAAGAAGGT GAAATCGTGC 
AGCGTGTGCA AGCAGGCCAT CACCAGAGAG GGCAGTGCGT GCCGGGTTTG CCGATTGTCG 
TGCCACAGAA AATGTGAAGC CAAGATTGCG TCGCCCTGTA CTCCTCGGGT CAATTATGAG 
CTGCCGTCAA GCTCTGAAAG CCCCCAGAAG AATGTAGAAA CCACAGGATC CACCAAATCC 
TCCAGAAGCA CTCAGTCCCG CCGCAGACCA TCAAGGAGCA TTAGCCTGAT TCACGCCATG 
GAGGACAGCT GCGAGTTGGA CCTCACCTAC ATCACCGAGC GGATCATCTC CGTCACCTTC 
TCCACCGGGA CGGAAGAGCC CACCTTCTGC CGGAACCTGA AGGAAGTGGC GCATATGCTG 
AAGTCCAAAC ATGGAGACAA TTACCTGATC TTCAACTTGT CGGAACGCAG ACATGACATC 
AGCAAGCTCC ATTCCAAGGT GTTGGATTTC GGATGGCCGG ACCTGCACGC GCCGGCCTTG 
GAAAAAGTCT GCAGCATCTG CAAAGCCATG GACACCTGGC TCAACGCCGA CCCTCACAAC 
GTCGTCGTCA TCCATAACAA AGGCAACCGA GGGCGGACAG GTGTGGTGAT TGCCGCCTAT 
ATGCACTACA GCAACATATC TGCCAGTGCA GACCAAGCCT TGGACAGGTT TGCCATGAGA 
AGGTTCTATG AAGACAAAGT CCTTCCAGTA GCTCAACCTT CCCAAAAAAG GTATATAAAC 
TACTTCAGTG GGCTTCTCTC TGGCGCCATG AAAATGAACA GCAAACCTCT CTTCCTGCAT 
CATGTGATCA TGCATGGGAT CCCCAACTTT GAGTCCAAGG GAGGTTGTCG GCCATTTCTG 
AAGATCTACC AAGCGATGCA GCCTGTATAC ACCTCGGGCA TCTACAACGT GCAAGGGGAC 
AGCCAGACGA GCATCTGCAT CACCATAGAA CCGGGGCTGT TACTAAAAGG TGACATCTTG 
CTCAAGTGCT ACCACAAGAA GTTCCGCAGC CCCACGCGGG ACGTCATATT CCGGGTGCAG 
TTCCATACGT GCGCCATCCA TGATCTGGGA ATCGTGTTTG GGAAGGAGGA TCTGGACGAA 
GCTTTCAAAG ATGAGCGTTT TCCCGAATAT GGAAAAGTGG AATTTGTTTT TTCATATGGA 
CCAGAAAAGA TACAAGGCAT GGAGCACCTG GAGAATGGAC CCAGCGTCTC TGTGGATTAT 
AACACATCCG ACCCTCTGAT TCGCTGGGAC TCCTATGATA ACTTCAACGT GCACCGGGAA 
GACAGCCTGG AAGAGGTTGG ACACACTCAA GGCCCCTTGG ATGGAAGCCT GTACGCCAAG 
GTGAAGAAGA AAGACTCTAC GAACGGCAGC ACCAGCACCG TCAACGCCAG CGGAATTCCG 
TTGTCTGCCG CGCCCAATCA CATTGAACAC ACCCTGTCCG TGAGCAGCGA CTCCGGCAAC 
TCGACTGCTT CGACCAAAAC CGACAAGACC GATGAGCAAG TGGCCACGGC GGCTGCCAAC 
CAAGGGTTGA CCCCTGAGGA GAAGCAAGAG TTGGAGCGGC TCCTGAGCGG CTTTGGCTTG 
GAGACCGAAG GGCAGATGCA CAATTTAAAT CCGGGCACAT CTGCAGCAGC AGCGATGCTT 
CACGTTGTGC CGGCGCAAGT CCACGTCAAT GGGGAACGGA CATCTCCGCA GGTGGAACGA 
GAGACAGATA TTCTTGATGA TGATCTGCCC AACCATGATG TTCACAGTGT TGGAAGCTTG 
GGGACCCTGT CTTCGTGTGA TGTGATTCCA AGCTTGAGTG AGACTGGCTA CCAGGATTCT 
CACCTTCGCA TGTCCCCCTT GACCAATGGT CCAGCCACTG TTAATGGCAT TGATAACCTG 
CAAAAACATG GGTTTATTGG TAAAGAGACG ATGATTAATG GCGGCTACCC GTACAACAAC 
CAGAATGTCT TAAAAAGCGC TTTGATCCCT CATTCCCAGG ATGCAGGGAT CCATATGCGA 
CCTTCCGTGT CTGCTCATGA AAACTTAGCC TATCAAGTCA ACCAGCCCAA CTGGGGACAA 
CCTTCTCCAT CAAATGTAAC ACAAAAGCAC TTATACTATT ATGACCCGAA TGGCATGTAC 
CGTTCACAGT CGTACACCGC TGCCGAACCA GGGAAATTCG AAAACATTTC TCAACTACCC 
CAAGCTCCAG CCCGTAGCGT TAGTAGCAGA GAGGCGGTGC AGAGGGGCCT TAACTCGTGG 
CAGCAAAGTG GAAGCAGGCC CCCATCCCGG GATGGAACTA CTGAGAATCA AGTTGCCAGT 
CCTAGCCCGC AGCCAAGCAC TTTCCAGACT GTTCCACAGA GCCACAGCAT CCCAGAATTC 
CCCAAAATGG CTTCCCAGAA GGAGATTGAG CAGTCTATAG AAGCACTTAA TATGCTTATG 
CTGGACCTGG ACCCTTCATT CTCACAAATG CACAAGTCTC AGAGTGTGCC TGTTGCCCCT 
AGAGAAGAAA GGGTTCTGCC AGTGCAAGGA TCCTTCTCAG CGCAGCCATC TTTGGGCATT 
TATAGCCAGC CGACTCCACA GGTGGCTACA CAAAGGCCTA GCAACGCTTA CGTGGCTCCT 
AGCATGGTGC AGAGCGCTGG AGCCTCTCAA AGCCAACCAC ACTTGCCTTC GGAACAGGTT 
TCGAGAACAT ATTCACCTGG GGTGCATCAT GACCAAGCTC GGGCTGTTGG GCCAGAAAAA 
GTTCTGAATG ACTATCGGGA GCCTTATGCT GCTGGAACCT TCTCTCCTTA TGGTTATCAG 
AACCAACAAC CGCCTTTGGC ATTGACAAGA AGCTATAGCT ATGGAAGTAG TGCTTCGCCA 
TCTTCGGTTC CACCATTATC GGCATATATG CAGAGCAATA CTCAGCAACC CGTCCTTTCT 
TCTCCTGCTT CTTCTGTGGT CATGGCTCAA CCTCAGCCTC AATCCCAGTC TCCGACCAGA 
GAGCCAGACG ATGAACAGTA CAGTCTGGAA GGGCTGGTAG CTCACAGAAT TGCTGAGTAC 
AACAACAAAC TCCGGAATAT CCGTAAAAGC ATGAATAGCC CCCATCCCCA TGGCGTGCAG 
CGCCAGCGTT CCTTCACTTT CTCTGGGGTG CACACTCGGG ACAAGACTCC GGATGAGATC 
GGGTCCCTGG CACGGAGGCG CACCACCAGC GAGGGGCAAT ATGACGAGGG CAATGCTGAC 
CTGGGCAGAG CGAGTAGCCC CACCAAGGTG CGCTCCCCAG TGCGCTGCGT CTCCCCGGAA 
GTCGCCTTCA CCATTGCGGC AAACCCAGGG GGGAGACCCA AGGAGCCCCA TCTGCACAGC 
TACAAGGAGG CCTTTGAAGA GATGGAAATG ACGCCGCCAG TGATCAGCCC GGGGGCCACA 
GTAGCCCGAT CCCCCCCTGG TTTAGCCAAG ACCCCCCTGT CTGCCCTGGG ACTGAAGCCC 
CACAATCCCG CAGAAATCGT TCTCCAGCAA TCGGGAGAGC CCAGGAGTTA TGTTGAATCC 
GTTGCCCGGA CGGCTGCCGC GGGAGGAGTC GCACCCCCAT CACCTACAGG CCCCCAGGCA 
TATAGTGTCG ATTCTCTGAT GAGAAATGGG GGTTACCCCA ATGTTTTCAC CTCTCCCAGC 
CCCATTTCTA CCAGCAGCCC CATACACAGC ACTGATGGCT CCCTAAGGAG TTACCCGTCG 
GTGGAGAGCG GCGCCCGTGT CCCCACTCCC ACTCAGCCTT CCGTGGATTC CAGCTTCAGA 
TCCACCAGTC TATCGCAGCC GTCGCCCCAA AGCAGTTACC AGAACGCCTC TCCCATGGTT 
CCCTACGCTG GCACACCGGC CAGTTCCTAT GATGGATACC CTTATTCCCC CAGCAGTGCC 
CTCACCCAGA CAGACGGCCT CTCCGGGCCA CAGATGAACG CGGTCATTGG TCACATGGTT 
CACGGAAGCC CCAAGGCCGT TCATAGAACG GTGGGGACCA ACACACCACC GAGTCCAGGT 
TTTGGGAGGA GAATGGTCAC CCCCAATATG GCGCCTCCAC CCGGAAGTCC AAGCATGGGC 
AGACACCCCA TGGCTACACA TATGGCTGCA CCCGTTACAG TAGGTAGCCC CAGCCTTCCT 
CTCCACCAGG CCTTGATGAC GGGAAATGCT CCGGTGACTC CTGGTAGCCC CGGCTTGGAG 
AGACACGTTA TGTATGGCTA TTCCCCCGCT GATGACAAAC GACCGACACT GTCACGGCAG 
AGCAGTGCGT CCGGATACCA GGCACCGCCC ACCCCATCCT TCCCGGTCTC CCCGGCCTAT 
TACCATGGCA TGAGTAGCCC TTCCTCCTCC TCTCCTGACT CAACCTTATA CCGGCAAGGG 
AGCCCAATCT CACAGCCGTC TCTGCCCGAG AAGAGGAGAA TGTCATCTGG GGACAGATCC 
AACAGCCTCC CCAACTACTC CACCCTCAAT GGAAAAACCT CTTCGCCCAT GTCCAGCGGG 
ATGTCCAGCC CCAGCGGCGG AAGCACAATG GCATTTACTC ACACTCTACC GGACTTCTCC 
AAGTTCTCCA TGCCAGACAG CAGCCCGGAG ACCCGAGCAA ATGTGAAGTT TGTGCAGGAC 
ACGTCCAAGT ACTGGTACAA ACCAGACATT TCCAGGGATC AGGCCATCTC CCTGCTGAAG 
GACAAAGACC CCGGGGCGTT CATCATACGA GACAGCCATT CGTTCCGAGG GGCCTACGGA 
CTTGCCATGA AAGTTGCCAC CCCGCCCCCC AACGCTGGCC AGCCCAGCAA GAAGGGAGAT 
GTGACCAATG AGCTGGTTCG GCACTTTTTG ATTGAGACGA GTCCCAGGGG GGTGAAATTA 
AAGGGCTGCC AGAACGAACC CTACTTTGGC TGCCTGTCGG CTCTGGTGTA CCAGCACTCC 
ATTACTCCCC TTGCACTGCC CTGCAAACTG GTCATACCCT GCAGAGATCC CTCTGATGAG 
ACCAAGGAGA CGGCTGTTCC GGCCAATTCA GCCTCAGATT TACTGAAACA GGGCGCAGCC 
TGCAACGTGT TATTCATAAA CTCGATAGAC ATGGAGTCGC TGACCGGCCC ACAAGCCATT 
GCGAAGACCA TCGCCGAGTC TCTCGCCGCC GACCCCCCGC CGGCCGCCGC CATCGTTCAC 
TTCAAAGTCT CCGCCCAAGG AATCACCTTA ACCGACAACC AGAGAAAATT ATTTTTCAGA 
CGACATTATC CGCTCAGCTC AGTCACCTTC TGCGACCTGG ACCCGCAAGA GAGAAAGTGG 
ATGAAGGCCG ATGGGACACA AGCCAAACTC TTTGGCTTTG TGGCCAGAAA GCAAGGAAGC 
ACCACAGACA ATATGTGCCA CCTGTTTGCC GAACTTGACC CAAACCAACC AGCCACTGCC 
ATCGTCAACT TTGTGTCCCG AGTCATCGGC CTGCAGAAGA GATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031749631.1
CDS	248..5812
Translation	MGCTVSFICCEEDPPLMSAEPQHEAETKEEKKRLLPKPEDLEGQNLHTFKVKTFKKVKSCSVCKQAITREGSACRVCRLSCHRKCEAKIASPCTPRVNYELPSSSESPQKNVETTGSTKSSRSTQSRRRPSRSISLIHAMEDSCELDLTYITERIISVTFSTGTEEPTFCRNLKEVAHMLKSKHGDNYLIFNLSERRHDISKLHSKVLDFGWPDLHAPALEKVCSICKAMDTWLNADPHNVVVIHNKGNRGRTGVVIAAYMHYSNISASADQALDRFAMRRFYEDKVLPVAQPSQKRYINYFSGLLSGAMKMNSKPLFLHHVIMHGIPNFESKGGCRPFLKIYQAMQPVYTSGIYNVQGDSQTSICITIEPGLLLKGDILLKCYHKKFRSPTRDVIFRVQFHTCAIHDLGIVFGKEDLDEAFKDERFPEYGKVEFVFSYGPEKIQGMEHLENGPSVSVDYNTSDPLIRWDSYDNFNVHREDSLEEVGHTQGPLDGSLYAKVKKKDSTNGSTSTVNASGIPLSAAPNHIEHTLSVSSDSGNSTASTKTDKTDEQVATAAANQGLTPEEKQELERLLSGFGLETEGQMHNLNPGTSAAAAMLHVVPAQVHVNGERTSPQVERETDILDDDLPNHDVHSVGSLGTLSSCDVIPSLSETGYQDSHLRMSPLTNGPATVNGIDNLQKHGFIGKETMINGGYPYNNQNVLKSALIPHSQDAGIHMRPSVSAHENLAYQVNQPNWGQPSPSNVTQKHLYYYDPNGMYRSQSYTAAEPGKFENISQLPQAPARSVSSREAVQRGLNSWQQSGSRPPSRDGTTENQVASPSPQPSTFQTVPQSHSIPEFPKMASQKEIEQSIEALNMLMLDLDPSFSQMHKSQSVPVAPREERVLPVQGSFSAQPSLGIYSQPTPQVATQRPSNAYVAPSMVQSAGASQSQPHLPSEQVSRTYSPGVHHDQARAVGPEKVLNDYREPYAAGTFSPYGYQNQQPPLALTRSYSYGSSASPSSVPPLSAYMQSNTQQPVLSSPASSVVMAQPQPQSQSPTREPDDEQYSLEGLVAHRIAEYNNKLRNIRKSMNSPHPHGVQRQRSFTFSGVHTRDKTPDEIGSLARRRTTSEGQYDEGNADLGRASSPTKVRSPVRCVSPEVAFTIAANPGGRPKEPHLHSYKEAFEEMEMTPPVISPGATVARSPPGLAKTPLSALGLKPHNPAEIVLQQSGEPRSYVESVARTAAAGGVAPPSPTGPQAYSVDSLMRNGGYPNVFTSPSPISTSSPIHSTDGSLRSYPSVESGARVPTPTQPSVDSSFRSTSLSQPSPQSSYQNASPMVPYAGTPASSYDGYPYSPSSALTQTDGLSGPQMNAVIGHMVHGSPKAVHRTVGTNTPPSPGFGRRMVTPNMAPPPGSPSMGRHPMATHMAAPVTVGSPSLPLHQALMTGNAPVTPGSPGLERHVMYGYSPADDKRPTLSRQSSASGYQAPPTPSFPVSPAYYHGMSSPSSSSPDSTLYRQGSPISQPSLPEKRRMSSGDRSNSLPNYSTLNGKTSSPMSSGMSSPSGGSTMAFTHTLPDFSKFSMPDSSPETRANVKFVQDTSKYWYKPDISRDQAISLLKDKDPGAFIIRDSHSFRGAYGLAMKVATPPPNAGQPSKKGDVTNELVRHFLIETSPRGVKLKGCQNEPYFGCLSALVYQHSITPLALPCKLVIPCRDPSDETKETAVPANSASDLLKQGAACNVLFINSIDMESLTGPQAIAKTIAESLAADPPPAAAIVHFKVSAQGITLTDNQRKLFFRRHYPLSSVTFCDLDPQERKWMKADGTQAKLFGFVARKQGSTTDNMCHLFAELDPNQPATAIVNFVSRVIGLQKR

Target ORF information:

RefSeq Version	XM_031893771.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis tensin 1 (tns1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031893771.1

ORF Insert Sequence:

ATGGGATGCA CCGTCAGCTT CATCTGCTGT GAAGAGGACC CCCCGCTCAT GTCTGCTGAG 
CCCCAACATG AGGCCGAAAC GAAGGAGGAA AAAAAGAGGC TCCTTCCCAA GCCTGAAGAC 
CTGGAAGGGC AGAACCTTCA TACATTCAAG GTGAAGACCT TTAAGAAGGT GAAATCGTGC 
AGCGTGTGCA AGCAGGCCAT CACCAGAGAG GGCAGTGCGT GCCGGGTTTG CCGATTGTCG 
TGCCACAGAA AATGTGAAGC CAAGATTGCG TCGCCCTGTA CTCCTCGGGT CAATTATGAG 
CTGCCGTCAA GCTCTGAAAG CCCCCAGAAG AATGTAGAAA CCACAGGATC CACCAAATCC 
TCCAGAAGCA CTCAGTCCCG CCGCAGACCA TCAAGGAGCA TTAGCCTGAT TCACGCCATG 
GAGGACAGCT GCGAGTTGGA CCTCACCTAC ATCACCGAGC GGATCATCTC CGTCACCTTC 
TCCACCGGGA CGGAAGAGCC CACCTTCTGC CGGAACCTGA AGGAAGTGGC GCATATGCTG 
AAGTCCAAAC ATGGAGACAA TTACCTGATC TTCAACTTGT CGGAACGCAG ACATGACATC 
AGCAAGCTCC ATTCCAAGGT GTTGGATTTC GGATGGCCGG ACCTGCACGC GCCGGCCTTG 
GAAAAAGTCT GCAGCATCTG CAAAGCCATG GACACCTGGC TCAACGCCGA CCCTCACAAC 
GTCGTCGTCA TCCATAACAA AGGCAACCGA GGGCGGACAG GTGTGGTGAT TGCCGCCTAT 
ATGCACTACA GCAACATATC TGCCAGTGCA GACCAAGCCT TGGACAGGTT TGCCATGAGA 
AGGTTCTATG AAGACAAAGT CCTTCCAGTA GCTCAACCTT CCCAAAAAAG GTATATAAAC 
TACTTCAGTG GGCTTCTCTC TGGCGCCATG AAAATGAACA GCAAACCTCT CTTCCTGCAT 
CATGTGATCA TGCATGGGAT CCCCAACTTT GAGTCCAAGG GAGGTTGTCG GCCATTTCTG 
AAGATCTACC AAGCGATGCA GCCTGTATAC ACCTCGGGCA TCTACAACGT GCAAGGGGAC 
AGCCAGACGA GCATCTGCAT CACCATAGAA CCGGGGCTGT TACTAAAAGG TGACATCTTG 
CTCAAGTGCT ACCACAAGAA GTTCCGCAGC CCCACGCGGG ACGTCATATT CCGGGTGCAG 
TTCCATACGT GCGCCATCCA TGATCTGGGA ATCGTGTTTG GGAAGGAGGA TCTGGACGAA 
GCTTTCAAAG ATGAGCGTTT TCCCGAATAT GGAAAAGTGG AATTTGTTTT TTCATATGGA 
CCAGAAAAGA TACAAGGCAT GGAGCACCTG GAGAATGGAC CCAGCGTCTC TGTGGATTAT 
AACACATCCG ACCCTCTGAT TCGCTGGGAC TCCTATGATA ACTTCAACGT GCACCGGGAA 
GACAGCCTGG AAGAGGTTGG ACACACTCAA GGCCCCTTGG ATGGAAGCCT GTACGCCAAG 
GTGAAGAAGA AAGACTCTAC GAACGGCAGC ACCAGCACCG TCAACGCCAG CGGAATTCCG 
TTGTCTGCCG CGCCCAATCA CATTGAACAC ACCCTGTCCG TGAGCAGCGA CTCCGGCAAC 
TCGACTGCTT CGACCAAAAC CGACAAGACC GATGAGCAAG TGGCCACGGC GGCTGCCAAC 
CAAGGGTTGA CCCCTGAGGA GAAGCAAGAG TTGGAGCGGC TCCTGAGCGG CTTTGGCTTG 
GAGACCGAAG GGCAGATGCA CAATTTAAAT CCGGGCACAT CTGCAGCAGC AGCGATGCTT 
CACGTTGTGC CGGCGCAAGT CCACGTCAAT GGGGAACGGA CATCTCCGCA GGTGGAACGA 
GAGACAGATA TTCTTGATGA TGATCTGCCC AACCATGATG TTCACAGTGT TGGAAGCTTG 
GGGACCCTGT CTTCGTGTGA TGTGATTCCA AGCTTGAGTG AGACTGGCTA CCAGGATTCT 
CACCTTCGCA TGTCCCCCTT GACCAATGGT CCAGCCACTG TTAATGGCAT TGATAACCTG 
CAAAAACATG GGTTTATTGG TAAAGAGACG ATGATTAATG GCGGCTACCC GTACAACAAC 
CAGAATGTCT TAAAAAGCGC TTTGATCCCT CATTCCCAGG ATGCAGGGAT CCATATGCGA 
CCTTCCGTGT CTGCTCATGA AAACTTAGCC TATCAAGTCA ACCAGCCCAA CTGGGGACAA 
CCTTCTCCAT CAAATGTAAC ACAAAAGCAC TTATACTATT ATGACCCGAA TGGCATGTAC 
CGTTCACAGT CGTACACCGC TGCCGAACCA GGGAAATTCG AAAACATTTC TCAACTACCC 
CAAGCTCCAG CCCGTAGCGT TAGTAGCAGA GAGGCGGTGC AGAGGGGCCT TAACTCGTGG 
CAGCAAAGTG GAAGCAGGCC CCCATCCCGG GATGGAACTA CTGAGAATCA AGTTGCCAGT 
CCTAGCCCGC AGCCAAGCAC TTTCCAGACT GTTCCACAGA GCCACAGCAT CCCAGAATTC 
CCCAAAATGG CTTCCCAGAA GGAGATTGAG CAGTCTATAG AAGCACTTAA TATGCTTATG 
CTGGACCTGG ACCCTTCATT CTCACAAATG CACAAGTCTC AGAGTGTGCC TGTTGCCCCT 
AGAGAAGAAA GGGTTCTGCC AGTGCAAGGA TCCTTCTCAG CGCAGCCATC TTTGGGCATT 
TATAGCCAGC CGACTCCACA GGTGGCTACA CAAAGGCCTA GCAACGCTTA CGTGGCTCCT 
AGCATGGTGC AGAGCGCTGG AGCCTCTCAA AGCCAACCAC ACTTGCCTTC GGAACAGGTT 
TCGAGAACAT ATTCACCTGG GGTGCATCAT GACCAAGCTC GGGCTGTTGG GCCAGAAAAA 
GTTCTGAATG ACTATCGGGA GCCTTATGCT GCTGGAACCT TCTCTCCTTA TGGTTATCAG 
AACCAACAAC CGCCTTTGGC ATTGACAAGA AGCTATAGCT ATGGAAGTAG TGCTTCGCCA 
TCTTCGGTTC CACCATTATC GGCATATATG CAGAGCAATA CTCAGCAACC CGTCCTTTCT 
TCTCCTGCTT CTTCTGTGGT CATGGCTCAA CCTCAGCCTC AATCCCAGTC TCCGACCAGA 
GAGCCAGACG ATGAACAGTA CAGTCTGGAA GGGCTGGTAG CTCACAGAAT TGCTGAGTAC 
AACAACAAAC TCCGGAATAT CCGTAAAAGC ATGAATAGCC CCCATCCCCA TGGCGTGCAG 
CGCCAGCGTT CCTTCACTTT CTCTGGGGTG CACACTCGGG ACAAGACTCC GGATGAGATC 
GGGTCCCTGG CACGGAGGCG CACCACCAGC GAGGGGCAAT ATGACGAGGG CAATGCTGAC 
CTGGGCAGAG CGAGTAGCCC CACCAAGGTG CGCTCCCCAG TGCGCTGCGT CTCCCCGGAA 
GTCGCCTTCA CCATTGCGGC AAACCCAGGG GGGAGACCCA AGGAGCCCCA TCTGCACAGC 
TACAAGGAGG CCTTTGAAGA GATGGAAATG ACGCCGCCAG TGATCAGCCC GGGGGCCACA 
GTAGCCCGAT CCCCCCCTGG TTTAGCCAAG ACCCCCCTGT CTGCCCTGGG ACTGAAGCCC 
CACAATCCCG CAGAAATCGT TCTCCAGCAA TCGGGAGAGC CCAGGAGTTA TGTTGAATCC 
GTTGCCCGGA CGGCTGCCGC GGGAGGAGTC GCACCCCCAT CACCTACAGG CCCCCAGGCA 
TATAGTGTCG ATTCTCTGAT GAGAAATGGG GGTTACCCCA ATGTTTTCAC CTCTCCCAGC 
CCCATTTCTA CCAGCAGCCC CATACACAGC ACTGATGGCT CCCTAAGGAG TTACCCGTCG 
GTGGAGAGCG GCGCCCGTGT CCCCACTCCC ACTCAGCCTT CCGTGGATTC CAGCTTCAGA 
TCCACCAGTC TATCGCAGCC GTCGCCCCAA AGCAGTTACC AGAACGCCTC TCCCATGGTT 
CCCTACGCTG GCACACCGGC CAGTTCCTAT GATGGATACC CTTATTCCCC CAGCAGTGCC 
CTCACCCAGA CAGACGGCCT CTCCGGGCCA CAGATGAACG CGGTCATTGG TCACATGGTT 
CACGGAAGCC CCAAGGCCGT TCATAGAACG GTGGGGACCA ACACACCACC GAGTCCAGGT 
TTTGGGAGGA GAATGGTCAC CCCCAATATG GCGCCTCCAC CCGGAAGTCC AAGCATGGGC 
AGACACCCCA TGGCTACACA TATGGCTGCA CCCGTTACAG TAGGTAGCCC CAGCCTTCCT 
CTCCACCAGG CCTTGATGAC GGGAAATGCT CCGGTGACTC CTGGTAGCCC CGGCTTGGAG 
AGACACGTTA TGTATGGCTA TTCCCCCGCT GATGACAAAC GACCGACACT GTCACGGCAG 
AGCAGTGCGT CCGGATACCA GGCACCGCCC ACCCCATCCT TCCCGGTCTC CCCGGCCTAT 
TACCATGGCA TGAGTAGCCC TTCCTCCTCC TCTCCTGACT CAACCTTATA CCGGCAAGGG 
AGCCCAATCT CACAGCCGTC TCTGCCCGAG AAGAGGAGAA TGTCATCTGG GGACAGATCC 
AACAGCCTCC CCAACTACTC CACCCTCAAT GGAAAAACCT CTTCGCCCAT GTCCAGCGGG 
ATGTCCAGCC CCAGCGGCGG AAGCACAATG GCATTTACTC ACACTCTACC GGACTTCTCC 
AAGTTCTCCA TGCCAGACAG CAGCCCGGAG ACCCGAGCAA ATGTGAAGTT TGTGCAGGAC 
ACGTCCAAGT ACTGGTACAA ACCAGACATT TCCAGGGATC AGGCCATCTC CCTGCTGAAG 
GACAAAGACC CCGGGGCGTT CATCATACGA GACAGCCATT CGTTCCGAGG GGCCTACGGA 
CTTGCCATGA AAGTTGCCAC CCCGCCCCCC AACGCTGGCC AGCCCAGCAA GAAGGGAGAT 
GTGACCAATG AGCTGGTTCG GCACTTTTTG ATTGAGACGA GTCCCAGGGG GGTGAAATTA 
AAGGGCTGCC AGAACGAACC CTACTTTGGC TGCCTGTCGG CTCTGGTGTA CCAGCACTCC 
ATTACTCCCC TTGCACTGCC CTGCAAACTG GTCATACCCT GCAGAGATCC CTCTGATGAG 
ACCAAGGAGA CGGCTGTTCC GGCCAATTCA GCCTCAGATT TACTGAAACA GGGCGCAGCC 
TGCAACGTGT TATTCATAAA CTCGATAGAC ATGGAGTCGC TGACCGGCCC ACAAGCCATT 
GCGAAGACCA TCGCCGAGTC TCTCGCCGCC GACCCCCCGC CGGCCGCCGC CATCGTTCAC 
TTCAAAGTCT CCGCCCAAGG AATCACCTTA ACCGACAACC AGAGAAAATT ATTTTTCAGA 
CGACATTATC CGCTCAGCTC AGTCACCTTC TGCGACCTGG ACCCGCAAGA GAGAAAGTGG 
ATGAAGGCCG ATGGGACACA AGCCAAACTC TTTGGCTTTG TGGCCAGAAA GCAAGGAAGC 
ACCACAGACA ATATGTGCCA CCTGTTTGCC GAACTTGACC CAAACCAACC AGCCACTGCC 
ATCGTCAACT TTGTGTCCCG AGTCATCGGC CTGCAGAAGA GATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

tns1 ( XM_031893772.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_031749632.1 Xenopus tropicalis tensin 1 (tns1), transcript variant X4, mRNA.

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CloneID	OXa67404
Clone ID Related Accession (Same CDS sequence)	XM_031893772.1
Accession Version	XM_031893772.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5511bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1576512000000
Organism	Xenopus tropicalis(tropical clawed frog)
Product	tensin-1 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030685.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGATGCA CCGTCAGCTT CATCTGCTGT GAAGAGGACC CCCCGCTCAT GTCTGCTGAG 
CCCCAACATG AGGCCGAAAC GAAGGAGGAA AAAAAGAGGC TCCTTCCCAA GCCTGAAGAC 
CTGGAAGGGC AGAACCTTCA TACATTCAAG GTGAAGACCT TTAAGAAGGT GAAATCGTGC 
AGCGTGTGCA AGCAGGCCAT CACCAGAGAG GGCAGTGCGT GCCGGGTTTG CCGATTGTCG 
TGCCACAGAA AATGTGAAGC CAAGATTGCG TCGCCCTGTA CTCCTCGGGT CAATTATGAG 
CTGCCGTCAA GCTCTGAAAG CCCCCAGAAG AATGTAGAAA CCACAGGATC CACCAAATCC 
TCCAGAAGCA CTCAGTCCCG CCGCAGACCA TCAAGGAGCA TTAGCCTGAT TCACGCCATG 
GAGGACAGCT GCGAGTTGGA CCTCACCTAC ATCACCGAGC GGATCATCTC CGTCACCTTC 
TCCACCGGGA CGGAAGAGCC CACCTTCTGC CGGAACCTGA AGGAAGTGGC GCATATGCTG 
AAGTCCAAAC ATGGAGACAA TTACCTGATC TTCAACTTGT CGGAACGCAG ACATGACATC 
AGCAAGCTCC ATTCCAAGGT GTTGGATTTC GGATGGCCGG ACCTGCACGC GCCGGCCTTG 
GAAAAAGTCT GCAGCATCTG CAAAGCCATG GACACCTGGC TCAACGCCGA CCCTCACAAC 
GTCGTCGTCA TCCATAACAA AGGCAACCGA GGGCGGACAG GTGTGGTGAT TGCCGCCTAT 
ATGCACTACA GCAACATATC TGCCAGTGCA GACCAAGCCT TGGACAGGTT TGCCATGAGA 
AGGTTCTATG AAGACAAAGT CCTTCCAGTA GCTCAACCTT CCCAAAAAAG GTATATAAAC 
TACTTCAGTG GGCTTCTCTC TGGCGCCATG AAAATGAACA GCAAACCTCT CTTCCTGCAT 
CATGTGATCA TGCATGGGAT CCCCAACTTT GAGTCCAAGG GAGGTTGTCG GCCATTTCTG 
AAGATCTACC AAGCGATGCA GCCTGTATAC ACCTCGGGCA TCTACAACGT GCAAGGGGAC 
AGCCAGACGA GCATCTGCAT CACCATAGAA CCGGGGCTGT TACTAAAAGG TGACATCTTG 
CTCAAGTGCT ACCACAAGAA GTTCCGCAGC CCCACGCGGG ACGTCATATT CCGGGTGCAG 
TTCCATACGT GCGCCATCCA TGATCTGGGA ATCGTGTTTG GGAAGGAGGA TCTGGACGAA 
GCTTTCAAAG ATGAGCGTTT TCCCGAATAT GGAAAAGTGG AATTTGTTTT TTCATATGGA 
CCAGAAAAGA TACAAGGCAT GGAGCACCTG GAGAATGGAC CCAGCGTCTC TGTGGATTAT 
AACACATCCG ACCCTCTGAT TCGCTGGGAC TCCTATGATA ACTTCAACGT GCACCGGGAA 
GACAGCCTGG AAGAGGTTGG ACACACTCAA GGCCCCTTGG ATGGAAGCCT GTACGCCAAG 
GTGAAGAAGA AAGACTCTAC GAACGGCAGC ACCAGCACCG TCAACGCCAG CGGAATTCCG 
TTGTCTGCCG CGCCCAATCA CATTGAACAC ACCCTGTCCG TGAGCAGCGA CTCCGGCAAC 
TCGACTGCTT CGACCAAAAC CGACAAGACC GATGAGCAAG TGGCCACGGC GGCTGCCAAC 
CAAGGGTTGA CCCCTGAGGA GAAGCAAGAG TTGGAGCGGC TCCTGAGCGG CTTTGGCTTG 
GAGACCGAAG GGCAGATGCA CAATTTAAAT CCGGGCACAT CTGCAGCAGC AGCGATGCTT 
CACGTTGTGC CGGCGCAAGT CCACGTCAAT GGGGAACGGA CATCTCCGCA GGTGGAACGA 
GAGACAGATA TTCTTGATGA TGATCTGCCC AACCATGATG TTCACAGTGT TGGAAGCTTG 
GGGACCCTGT CTTCGTGTGA TGTGATTCCA AGCTTGAGTG AGACTGGCTA CCAGGATTCT 
CACCTTCGCA TGTCCCCCTT GACCAATGGT CCAGCCACTG TTAATGGCAT TGATAACCTG 
CAAAAACATG GGTTTATTGG TAAAGAGACG ATGATTAATG GCGGCTACCC GTACAACAAC 
CAGAATGTCT TAAAAAGCGC TTTGATCCCT CATTCCCAGG ATGCAGGGAT CCATATGCGA 
CCTTCCGTGT CTGCTCATGA AAACTTAGCC TATCAAGTCA ACCAGCCCAA CTGGGGACAA 
CCTTCTCCAT CAAATGTAAC ACAAAAGCAC TTATACTATT ATGACCCGAA TGGCATGTAC 
CGTTCACAGT CGTACACCGC TGCCGAACCA GGGAAATTCG AAAACATTTC TCAACTACCC 
CAAGCTCCAG CCCGTAGCGT TAGTAGCAGA GAGGCGGTGC AGAGGGGCCT TAACTCGTGG 
CAGCAAAGTG GAAGCAGGCC CCCATCCCGG GATGGAACTA CTGAGAATCA AGTTGCCAGT 
CCTAGCCCGC AGCCAAGCAC TTTCCAGACT GTTCCACAGA GCCACAGCAT CCCAGAATTC 
CCCAAAATGG CTTCCCAGAA GGAGATTGAG CAGTCTATAG AAGCACTTAA TATGCTTATG 
CTGGACCTGG ACCCTTCATT CTCACAAATG CACAAGTCTC AGAGTGTGCC TGTTGCCCCT 
AGAGAAGAAA GGGTTCTGCC AGTGCAAGGA TCCTTCTCAG CGCAGCCATC TTTGGGCATT 
TATAGCCAGC CGACTCCACA GGTGGCTACA CAAAGGCCTA GCAACGCTTA CGTGGCTCCT 
AGCATGGTGC AGAGCGCTGG AGCCTCTCAA AGCCAACCAC ACTTGCCTTC GGAACAGGTT 
TCGAGAACAT ATTCACCTGG GGTGCATCAT GACCAAGCTC GGGCTGTTGG GCCAGAAAAA 
GTTCTGAATG ACTATCGGGA GCCTTATGCT GCTGGAACCT TCTCTCCTTA TGGTTATCAG 
AACCAACAAC CGCCTTTGGC ATTGACAAGA AGCTATAGCT ATGGAAGTAG TGCTTCGCCA 
TCTTCGGTTC CACCATTATC GGCATATATG CAGAGCAATA CTCAGCAACC CGTCCTTTCT 
TCTCCTGCTT CTTCTGTGGT CATGGCTCAA CCTCAGCCTC AATCCCAGTC TCCGACCAGA 
GAGCCAGACG ATGAACAGTA CAGTCTGGAA GGGCTGGTAG CTCACAGAAT TGCTGGGGTG 
CACACTCGGG ACAAGACTCC GGATGAGATC GGGTCCCTGG CACGGAGGCG CACCACCAGC 
GAGGGGCAAT ATGACGAGGG CAATGCTGAC CTGGGCAGAG CGAGTAGCCC CACCAAGGTG 
CGCTCCCCAG TGCGCTGCGT CTCCCCGGAA GTCGCCTTCA CCATTGCGGC AAACCCAGGG 
GGGAGACCCA AGGAGCCCCA TCTGCACAGC TACAAGGAGG CCTTTGAAGA GATGGAAATG 
ACGCCGCCAG TGATCAGCCC GGGGGCCACA GTAGCCCGAT CCCCCCCTGG TTTAGCCAAG 
ACCCCCCTGT CTGCCCTGGG ACTGAAGCCC CACAATCCCG CAGAAATCGT TCTCCAGCAA 
TCGGGAGAGA CAGTCAGGGA AACAGTAATT GCTTACATGG AAGAGCCCAG GAGTTATGTT 
GAATCCGTTG CCCGGACGGC TGCCGCGGGA GGAGTCGCAC CCCCATCACC TACAGGCCCC 
CAGGCATATA GTGTCGATTC TCTGATGAGA AATGGGGGTT ACCCCAATGT TTTCACCTCT 
CCCAGCCCCA TTTCTACCAG CAGCCCCATA CACAGCACTG ATGGCTCCCT AAGGAGTTAC 
CCGTCGGTGG AGAGCGGCGC CCGTGTCCCC ACTCCCACTC AGCCTTCCGT GGATTCCAGC 
TTCAGATCCA CCAGTCTATC GCAGCCGTCG CCCCAAAGCA GTTACCAGAA CGCCTCTCCC 
ATGGTTCCCT ACGCTGGCAC ACCGGCCAGT TCCTATGATG GATACCCTTA TTCCCCCAGC 
AGTGCCCTCA CCCAGACAGA CGGCCTCTCC GGGCCACAGA TGAACGCGGT CATTGGTCAC 
ATGGTTCACG GAAGCCCCAA GGCCGTTCAT AGAACGGTGG GGACCAACAC ACCACCGAGT 
CCAGGTTTTG GGAGGAGAAT GGTCACCCCC AATATGGCGC CTCCACCCGG AAGTCCAAGC 
ATGGGCAGAC ACCCCATGGC TACACATATG GCTGCACCCG TTACAGTAGG TAGCCCCAGC 
CTTCCTCTCC ACCAGGCCTT GATGACGGGA AATGCTCCGG TGACTCCTGG TAGCCCCGGC 
TTGGAGAGAC ACGTTATGTA TGGCTATTCC CCCGCTGATG ACAAACGACC GACACTGTCA 
CGGCAGAGCA GTGCGTCCGG ATACCAGGCA CCGCCCACCC CATCCTTCCC GGTCTCCCCG 
GCCTATTACC ATGGCATGAG TAGCCCTTCC TCCTCCTCTC CTGACTCAAC CTTATACCGG 
CAAGGGAGCC CAATCTCACA GCCGTCTCTG CCCGAGAAGA GGAGAATGTC ATCTGGGGAC 
AGATCCAACA GCCTCCCCAA CTACTCCACC CTCAATGGAA AAACCTCTTC GCCCATGTCC 
AGCGGGATGT CCAGCCCCAG CGGCGGAAGC ACAATGGCAT TTACTCACAC TCTACCGGAC 
TTCTCCAAGT TCTCCATGCC AGACAGCAGC CCGGAGACCC GAGCAAATGT GAAGTTTGTG 
CAGGACACGT CCAAGTACTG GTACAAACCA GACATTTCCA GGGATCAGGC CATCTCCCTG 
CTGAAGGACA AAGACCCCGG GGCGTTCATC ATACGAGACA GCCATTCGTT CCGAGGGGCC 
TACGGACTTG CCATGAAAGT TGCCACCCCG CCCCCCAACG CTGGCCAGCC CAGCAAGAAG 
GGAGATGTGA CCAATGAGCT GGTTCGGCAC TTTTTGATTG AGACGAGTCC CAGGGGGGTG 
AAATTAAAGG GCTGCCAGAA CGAACCCTAC TTTGGCTGCC TGTCGGCTCT GGTGTACCAG 
CACTCCATTA CTCCCCTTGC ACTGCCCTGC AAACTGGTCA TACCCTGCAG AGATCCCTCT 
GATGAGACCA AGGAGACGGC TGTTCCGGCC AATTCAGCCT CAGATTTACT GAAACAGGGC 
GCAGCCTGCA ACGTGTTATT CATAAACTCG ATAGACATGG AGTCGCTGAC CGGCCCACAA 
GCCATTGCGA AGACCATCGC CGAGTCTCTC GCCGCCGACC CCCCGCCGGC CGCCGCCATC 
GTTCACTTCA AAGTCTCCGC CCAAGGAATC ACCTTAACCG ACAACCAGAG AAAATTATTT 
TTCAGACGAC ATTATCCGCT CAGCTCAGTC ACCTTCTGCG ACCTGGACCC GCAAGAGAGA 
AAGTGGATGA AGGCCGATGG GACACAAGCC AAACTCTTTG GCTTTGTGGC CAGAAAGCAA 
GGAAGCACCA CAGACAATAT GTGCCACCTG TTTGCCGAAC TTGACCCAAA CCAACCAGCC 
ACTGCCATCG TCAACTTTGT GTCCCGAGTC ATCGGCCTGC AGAAGAGATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031749632.1
CDS	247..5757
Translation	MGCTVSFICCEEDPPLMSAEPQHEAETKEEKKRLLPKPEDLEGQNLHTFKVKTFKKVKSCSVCKQAITREGSACRVCRLSCHRKCEAKIASPCTPRVNYELPSSSESPQKNVETTGSTKSSRSTQSRRRPSRSISLIHAMEDSCELDLTYITERIISVTFSTGTEEPTFCRNLKEVAHMLKSKHGDNYLIFNLSERRHDISKLHSKVLDFGWPDLHAPALEKVCSICKAMDTWLNADPHNVVVIHNKGNRGRTGVVIAAYMHYSNISASADQALDRFAMRRFYEDKVLPVAQPSQKRYINYFSGLLSGAMKMNSKPLFLHHVIMHGIPNFESKGGCRPFLKIYQAMQPVYTSGIYNVQGDSQTSICITIEPGLLLKGDILLKCYHKKFRSPTRDVIFRVQFHTCAIHDLGIVFGKEDLDEAFKDERFPEYGKVEFVFSYGPEKIQGMEHLENGPSVSVDYNTSDPLIRWDSYDNFNVHREDSLEEVGHTQGPLDGSLYAKVKKKDSTNGSTSTVNASGIPLSAAPNHIEHTLSVSSDSGNSTASTKTDKTDEQVATAAANQGLTPEEKQELERLLSGFGLETEGQMHNLNPGTSAAAAMLHVVPAQVHVNGERTSPQVERETDILDDDLPNHDVHSVGSLGTLSSCDVIPSLSETGYQDSHLRMSPLTNGPATVNGIDNLQKHGFIGKETMINGGYPYNNQNVLKSALIPHSQDAGIHMRPSVSAHENLAYQVNQPNWGQPSPSNVTQKHLYYYDPNGMYRSQSYTAAEPGKFENISQLPQAPARSVSSREAVQRGLNSWQQSGSRPPSRDGTTENQVASPSPQPSTFQTVPQSHSIPEFPKMASQKEIEQSIEALNMLMLDLDPSFSQMHKSQSVPVAPREERVLPVQGSFSAQPSLGIYSQPTPQVATQRPSNAYVAPSMVQSAGASQSQPHLPSEQVSRTYSPGVHHDQARAVGPEKVLNDYREPYAAGTFSPYGYQNQQPPLALTRSYSYGSSASPSSVPPLSAYMQSNTQQPVLSSPASSVVMAQPQPQSQSPTREPDDEQYSLEGLVAHRIAGVHTRDKTPDEIGSLARRRTTSEGQYDEGNADLGRASSPTKVRSPVRCVSPEVAFTIAANPGGRPKEPHLHSYKEAFEEMEMTPPVISPGATVARSPPGLAKTPLSALGLKPHNPAEIVLQQSGETVRETVIAYMEEPRSYVESVARTAAAGGVAPPSPTGPQAYSVDSLMRNGGYPNVFTSPSPISTSSPIHSTDGSLRSYPSVESGARVPTPTQPSVDSSFRSTSLSQPSPQSSYQNASPMVPYAGTPASSYDGYPYSPSSALTQTDGLSGPQMNAVIGHMVHGSPKAVHRTVGTNTPPSPGFGRRMVTPNMAPPPGSPSMGRHPMATHMAAPVTVGSPSLPLHQALMTGNAPVTPGSPGLERHVMYGYSPADDKRPTLSRQSSASGYQAPPTPSFPVSPAYYHGMSSPSSSSPDSTLYRQGSPISQPSLPEKRRMSSGDRSNSLPNYSTLNGKTSSPMSSGMSSPSGGSTMAFTHTLPDFSKFSMPDSSPETRANVKFVQDTSKYWYKPDISRDQAISLLKDKDPGAFIIRDSHSFRGAYGLAMKVATPPPNAGQPSKKGDVTNELVRHFLIETSPRGVKLKGCQNEPYFGCLSALVYQHSITPLALPCKLVIPCRDPSDETKETAVPANSASDLLKQGAACNVLFINSIDMESLTGPQAIAKTIAESLAADPPPAAAIVHFKVSAQGITLTDNQRKLFFRRHYPLSSVTFCDLDPQERKWMKADGTQAKLFGFVARKQGSTTDNMCHLFAELDPNQPATAIVNFVSRVIGLQKR

Target ORF information:

RefSeq Version	XM_031893772.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis tensin 1 (tns1), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031893772.1

ORF Insert Sequence:

ATGGGATGCA CCGTCAGCTT CATCTGCTGT GAAGAGGACC CCCCGCTCAT GTCTGCTGAG 
CCCCAACATG AGGCCGAAAC GAAGGAGGAA AAAAAGAGGC TCCTTCCCAA GCCTGAAGAC 
CTGGAAGGGC AGAACCTTCA TACATTCAAG GTGAAGACCT TTAAGAAGGT GAAATCGTGC 
AGCGTGTGCA AGCAGGCCAT CACCAGAGAG GGCAGTGCGT GCCGGGTTTG CCGATTGTCG 
TGCCACAGAA AATGTGAAGC CAAGATTGCG TCGCCCTGTA CTCCTCGGGT CAATTATGAG 
CTGCCGTCAA GCTCTGAAAG CCCCCAGAAG AATGTAGAAA CCACAGGATC CACCAAATCC 
TCCAGAAGCA CTCAGTCCCG CCGCAGACCA TCAAGGAGCA TTAGCCTGAT TCACGCCATG 
GAGGACAGCT GCGAGTTGGA CCTCACCTAC ATCACCGAGC GGATCATCTC CGTCACCTTC 
TCCACCGGGA CGGAAGAGCC CACCTTCTGC CGGAACCTGA AGGAAGTGGC GCATATGCTG 
AAGTCCAAAC ATGGAGACAA TTACCTGATC TTCAACTTGT CGGAACGCAG ACATGACATC 
AGCAAGCTCC ATTCCAAGGT GTTGGATTTC GGATGGCCGG ACCTGCACGC GCCGGCCTTG 
GAAAAAGTCT GCAGCATCTG CAAAGCCATG GACACCTGGC TCAACGCCGA CCCTCACAAC 
GTCGTCGTCA TCCATAACAA AGGCAACCGA GGGCGGACAG GTGTGGTGAT TGCCGCCTAT 
ATGCACTACA GCAACATATC TGCCAGTGCA GACCAAGCCT TGGACAGGTT TGCCATGAGA 
AGGTTCTATG AAGACAAAGT CCTTCCAGTA GCTCAACCTT CCCAAAAAAG GTATATAAAC 
TACTTCAGTG GGCTTCTCTC TGGCGCCATG AAAATGAACA GCAAACCTCT CTTCCTGCAT 
CATGTGATCA TGCATGGGAT CCCCAACTTT GAGTCCAAGG GAGGTTGTCG GCCATTTCTG 
AAGATCTACC AAGCGATGCA GCCTGTATAC ACCTCGGGCA TCTACAACGT GCAAGGGGAC 
AGCCAGACGA GCATCTGCAT CACCATAGAA CCGGGGCTGT TACTAAAAGG TGACATCTTG 
CTCAAGTGCT ACCACAAGAA GTTCCGCAGC CCCACGCGGG ACGTCATATT CCGGGTGCAG 
TTCCATACGT GCGCCATCCA TGATCTGGGA ATCGTGTTTG GGAAGGAGGA TCTGGACGAA 
GCTTTCAAAG ATGAGCGTTT TCCCGAATAT GGAAAAGTGG AATTTGTTTT TTCATATGGA 
CCAGAAAAGA TACAAGGCAT GGAGCACCTG GAGAATGGAC CCAGCGTCTC TGTGGATTAT 
AACACATCCG ACCCTCTGAT TCGCTGGGAC TCCTATGATA ACTTCAACGT GCACCGGGAA 
GACAGCCTGG AAGAGGTTGG ACACACTCAA GGCCCCTTGG ATGGAAGCCT GTACGCCAAG 
GTGAAGAAGA AAGACTCTAC GAACGGCAGC ACCAGCACCG TCAACGCCAG CGGAATTCCG 
TTGTCTGCCG CGCCCAATCA CATTGAACAC ACCCTGTCCG TGAGCAGCGA CTCCGGCAAC 
TCGACTGCTT CGACCAAAAC CGACAAGACC GATGAGCAAG TGGCCACGGC GGCTGCCAAC 
CAAGGGTTGA CCCCTGAGGA GAAGCAAGAG TTGGAGCGGC TCCTGAGCGG CTTTGGCTTG 
GAGACCGAAG GGCAGATGCA CAATTTAAAT CCGGGCACAT CTGCAGCAGC AGCGATGCTT 
CACGTTGTGC CGGCGCAAGT CCACGTCAAT GGGGAACGGA CATCTCCGCA GGTGGAACGA 
GAGACAGATA TTCTTGATGA TGATCTGCCC AACCATGATG TTCACAGTGT TGGAAGCTTG 
GGGACCCTGT CTTCGTGTGA TGTGATTCCA AGCTTGAGTG AGACTGGCTA CCAGGATTCT 
CACCTTCGCA TGTCCCCCTT GACCAATGGT CCAGCCACTG TTAATGGCAT TGATAACCTG 
CAAAAACATG GGTTTATTGG TAAAGAGACG ATGATTAATG GCGGCTACCC GTACAACAAC 
CAGAATGTCT TAAAAAGCGC TTTGATCCCT CATTCCCAGG ATGCAGGGAT CCATATGCGA 
CCTTCCGTGT CTGCTCATGA AAACTTAGCC TATCAAGTCA ACCAGCCCAA CTGGGGACAA 
CCTTCTCCAT CAAATGTAAC ACAAAAGCAC TTATACTATT ATGACCCGAA TGGCATGTAC 
CGTTCACAGT CGTACACCGC TGCCGAACCA GGGAAATTCG AAAACATTTC TCAACTACCC 
CAAGCTCCAG CCCGTAGCGT TAGTAGCAGA GAGGCGGTGC AGAGGGGCCT TAACTCGTGG 
CAGCAAAGTG GAAGCAGGCC CCCATCCCGG GATGGAACTA CTGAGAATCA AGTTGCCAGT 
CCTAGCCCGC AGCCAAGCAC TTTCCAGACT GTTCCACAGA GCCACAGCAT CCCAGAATTC 
CCCAAAATGG CTTCCCAGAA GGAGATTGAG CAGTCTATAG AAGCACTTAA TATGCTTATG 
CTGGACCTGG ACCCTTCATT CTCACAAATG CACAAGTCTC AGAGTGTGCC TGTTGCCCCT 
AGAGAAGAAA GGGTTCTGCC AGTGCAAGGA TCCTTCTCAG CGCAGCCATC TTTGGGCATT 
TATAGCCAGC CGACTCCACA GGTGGCTACA CAAAGGCCTA GCAACGCTTA CGTGGCTCCT 
AGCATGGTGC AGAGCGCTGG AGCCTCTCAA AGCCAACCAC ACTTGCCTTC GGAACAGGTT 
TCGAGAACAT ATTCACCTGG GGTGCATCAT GACCAAGCTC GGGCTGTTGG GCCAGAAAAA 
GTTCTGAATG ACTATCGGGA GCCTTATGCT GCTGGAACCT TCTCTCCTTA TGGTTATCAG 
AACCAACAAC CGCCTTTGGC ATTGACAAGA AGCTATAGCT ATGGAAGTAG TGCTTCGCCA 
TCTTCGGTTC CACCATTATC GGCATATATG CAGAGCAATA CTCAGCAACC CGTCCTTTCT 
TCTCCTGCTT CTTCTGTGGT CATGGCTCAA CCTCAGCCTC AATCCCAGTC TCCGACCAGA 
GAGCCAGACG ATGAACAGTA CAGTCTGGAA GGGCTGGTAG CTCACAGAAT TGCTGGGGTG 
CACACTCGGG ACAAGACTCC GGATGAGATC GGGTCCCTGG CACGGAGGCG CACCACCAGC 
GAGGGGCAAT ATGACGAGGG CAATGCTGAC CTGGGCAGAG CGAGTAGCCC CACCAAGGTG 
CGCTCCCCAG TGCGCTGCGT CTCCCCGGAA GTCGCCTTCA CCATTGCGGC AAACCCAGGG 
GGGAGACCCA AGGAGCCCCA TCTGCACAGC TACAAGGAGG CCTTTGAAGA GATGGAAATG 
ACGCCGCCAG TGATCAGCCC GGGGGCCACA GTAGCCCGAT CCCCCCCTGG TTTAGCCAAG 
ACCCCCCTGT CTGCCCTGGG ACTGAAGCCC CACAATCCCG CAGAAATCGT TCTCCAGCAA 
TCGGGAGAGA CAGTCAGGGA AACAGTAATT GCTTACATGG AAGAGCCCAG GAGTTATGTT 
GAATCCGTTG CCCGGACGGC TGCCGCGGGA GGAGTCGCAC CCCCATCACC TACAGGCCCC 
CAGGCATATA GTGTCGATTC TCTGATGAGA AATGGGGGTT ACCCCAATGT TTTCACCTCT 
CCCAGCCCCA TTTCTACCAG CAGCCCCATA CACAGCACTG ATGGCTCCCT AAGGAGTTAC 
CCGTCGGTGG AGAGCGGCGC CCGTGTCCCC ACTCCCACTC AGCCTTCCGT GGATTCCAGC 
TTCAGATCCA CCAGTCTATC GCAGCCGTCG CCCCAAAGCA GTTACCAGAA CGCCTCTCCC 
ATGGTTCCCT ACGCTGGCAC ACCGGCCAGT TCCTATGATG GATACCCTTA TTCCCCCAGC 
AGTGCCCTCA CCCAGACAGA CGGCCTCTCC GGGCCACAGA TGAACGCGGT CATTGGTCAC 
ATGGTTCACG GAAGCCCCAA GGCCGTTCAT AGAACGGTGG GGACCAACAC ACCACCGAGT 
CCAGGTTTTG GGAGGAGAAT GGTCACCCCC AATATGGCGC CTCCACCCGG AAGTCCAAGC 
ATGGGCAGAC ACCCCATGGC TACACATATG GCTGCACCCG TTACAGTAGG TAGCCCCAGC 
CTTCCTCTCC ACCAGGCCTT GATGACGGGA AATGCTCCGG TGACTCCTGG TAGCCCCGGC 
TTGGAGAGAC ACGTTATGTA TGGCTATTCC CCCGCTGATG ACAAACGACC GACACTGTCA 
CGGCAGAGCA GTGCGTCCGG ATACCAGGCA CCGCCCACCC CATCCTTCCC GGTCTCCCCG 
GCCTATTACC ATGGCATGAG TAGCCCTTCC TCCTCCTCTC CTGACTCAAC CTTATACCGG 
CAAGGGAGCC CAATCTCACA GCCGTCTCTG CCCGAGAAGA GGAGAATGTC ATCTGGGGAC 
AGATCCAACA GCCTCCCCAA CTACTCCACC CTCAATGGAA AAACCTCTTC GCCCATGTCC 
AGCGGGATGT CCAGCCCCAG CGGCGGAAGC ACAATGGCAT TTACTCACAC TCTACCGGAC 
TTCTCCAAGT TCTCCATGCC AGACAGCAGC CCGGAGACCC GAGCAAATGT GAAGTTTGTG 
CAGGACACGT CCAAGTACTG GTACAAACCA GACATTTCCA GGGATCAGGC CATCTCCCTG 
CTGAAGGACA AAGACCCCGG GGCGTTCATC ATACGAGACA GCCATTCGTT CCGAGGGGCC 
TACGGACTTG CCATGAAAGT TGCCACCCCG CCCCCCAACG CTGGCCAGCC CAGCAAGAAG 
GGAGATGTGA CCAATGAGCT GGTTCGGCAC TTTTTGATTG AGACGAGTCC CAGGGGGGTG 
AAATTAAAGG GCTGCCAGAA CGAACCCTAC TTTGGCTGCC TGTCGGCTCT GGTGTACCAG 
CACTCCATTA CTCCCCTTGC ACTGCCCTGC AAACTGGTCA TACCCTGCAG AGATCCCTCT 
GATGAGACCA AGGAGACGGC TGTTCCGGCC AATTCAGCCT CAGATTTACT GAAACAGGGC 
GCAGCCTGCA ACGTGTTATT CATAAACTCG ATAGACATGG AGTCGCTGAC CGGCCCACAA 
GCCATTGCGA AGACCATCGC CGAGTCTCTC GCCGCCGACC CCCCGCCGGC CGCCGCCATC 
GTTCACTTCA AAGTCTCCGC CCAAGGAATC ACCTTAACCG ACAACCAGAG AAAATTATTT 
TTCAGACGAC ATTATCCGCT CAGCTCAGTC ACCTTCTGCG ACCTGGACCC GCAAGAGAGA 
AAGTGGATGA AGGCCGATGG GACACAAGCC AAACTCTTTG GCTTTGTGGC CAGAAAGCAA 
GGAAGCACCA CAGACAATAT GTGCCACCTG TTTGCCGAAC TTGACCCAAA CCAACCAGCC 
ACTGCCATCG TCAACTTTGT GTCCCGAGTC ATCGGCCTGC AGAAGAGATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

tns1 ( XM_031893773.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_031749633.1 Xenopus tropicalis tensin 1 (tns1), transcript variant X5, mRNA.

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CloneID	OXa67405
Clone ID Related Accession (Same CDS sequence)	XM_031893773.1
Accession Version	XM_031893773.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5475bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1576512000000
Organism	Xenopus tropicalis(tropical clawed frog)
Product	tensin-1 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030685.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGATGCA CCGTCAGCTT CATCTGCTGT GAAGAGGACC CCCCGCTCAT GTCTGCTGAG 
CCCCAACATG AGGCCGAAAC GAAGGAGGAA AAAAAGAGGC TCCTTCCCAA GCCTGAAGAC 
CTGGAAGGGC AGAACCTTCA TACATTCAAG GTGAAGACCT TTAAGAAGGT GAAATCGTGC 
AGCGTGTGCA AGCAGGCCAT CACCAGAGAG GGCAGTGCGT GCCGGGTTTG CCGATTGTCG 
TGCCACAGAA AATGTGAAGC CAAGATTGCG TCGCCCTGTA CTCCTCGGGT CAATTATGAG 
CTGCCGTCAA GCTCTGAAAG CCCCCAGAAG AATGTAGAAA CCACAGGATC CACCAAATCC 
TCCAGAAGCA CTCAGTCCCG CCGCAGACCA TCAAGGAGCA TTAGCCTGAT TCACGCCATG 
GAGGACAGCT GCGAGTTGGA CCTCACCTAC ATCACCGAGC GGATCATCTC CGTCACCTTC 
TCCACCGGGA CGGAAGAGCC CACCTTCTGC CGGAACCTGA AGGAAGTGGC GCATATGCTG 
AAGTCCAAAC ATGGAGACAA TTACCTGATC TTCAACTTGT CGGAACGCAG ACATGACATC 
AGCAAGCTCC ATTCCAAGGT GTTGGATTTC GGATGGCCGG ACCTGCACGC GCCGGCCTTG 
GAAAAAGTCT GCAGCATCTG CAAAGCCATG GACACCTGGC TCAACGCCGA CCCTCACAAC 
GTCGTCGTCA TCCATAACAA AGGCAACCGA GGGCGGACAG GTGTGGTGAT TGCCGCCTAT 
ATGCACTACA GCAACATATC TGCCAGTGCA GACCAAGCCT TGGACAGGTT TGCCATGAGA 
AGGTTCTATG AAGACAAAGT CCTTCCAGTA GCTCAACCTT CCCAAAAAAG GTATATAAAC 
TACTTCAGTG GGCTTCTCTC TGGCGCCATG AAAATGAACA GCAAACCTCT CTTCCTGCAT 
CATGTGATCA TGCATGGGAT CCCCAACTTT GAGTCCAAGG GAGGTTGTCG GCCATTTCTG 
AAGATCTACC AAGCGATGCA GCCTGTATAC ACCTCGGGCA TCTACAACGT GCAAGGGGAC 
AGCCAGACGA GCATCTGCAT CACCATAGAA CCGGGGCTGT TACTAAAAGG TGACATCTTG 
CTCAAGTGCT ACCACAAGAA GTTCCGCAGC CCCACGCGGG ACGTCATATT CCGGGTGCAG 
TTCCATACGT GCGCCATCCA TGATCTGGGA ATCGTGTTTG GGAAGGAGGA TCTGGACGAA 
GCTTTCAAAG ATGAGCGTTT TCCCGAATAT GGAAAAGTGG AATTTGTTTT TTCATATGGA 
CCAGAAAAGA TACAAGGCAT GGAGCACCTG GAGAATGGAC CCAGCGTCTC TGTGGATTAT 
AACACATCCG ACCCTCTGAT TCGCTGGGAC TCCTATGATA ACTTCAACGT GCACCGGGAA 
GACAGCCTGG AAGAGGTTGG ACACACTCAA GGCCCCTTGG ATGGAAGCCT GTACGCCAAG 
GTGAAGAAGA AAGACTCTAC GAACGGCAGC ACCAGCACCG TCAACGCCAG CGGAATTCCG 
TTGTCTGCCG CGCCCAATCA CATTGAACAC ACCCTGTCCG TGAGCAGCGA CTCCGGCAAC 
TCGACTGCTT CGACCAAAAC CGACAAGACC GATGAGCAAG TGGCCACGGC GGCTGCCAAC 
CAAGGGTTGA CCCCTGAGGA GAAGCAAGAG TTGGAGCGGC TCCTGAGCGG CTTTGGCTTG 
GAGACCGAAG GGCAGATGCA CAATTTAAAT CCGGGCACAT CTGCAGCAGC AGCGATGCTT 
CACGTTGTGC CGGCGCAAGT CCACGTCAAT GGGGAACGGA CATCTCCGCA GGTGGAACGA 
GAGACAGATA TTCTTGATGA TGATCTGCCC AACCATGATG TTCACAGTGT TGGAAGCTTG 
GGGACCCTGT CTTCGTGTGA TGTGATTCCA AGCTTGAGTG AGACTGGCTA CCAGGATTCT 
CACCTTCGCA TGTCCCCCTT GACCAATGGT CCAGCCACTG TTAATGGCAT TGATAACCTG 
CAAAAACATG GGTTTATTGG TAAAGAGACG ATGATTAATG GCGGCTACCC GTACAACAAC 
CAGAATGTCT TAAAAAGCGC TTTGATCCCT CATTCCCAGG ATGCAGGGAT CCATATGCGA 
CCTTCCGTGT CTGCTCATGA AAACTTAGCC TATCAAGTCA ACCAGCCCAA CTGGGGACAA 
CCTTCTCCAT CAAATGTAAC ACAAAAGCAC TTATACTATT ATGACCCGAA TGGCATGTAC 
CGTTCACAGT CGTACACCGC TGCCGAACCA GGGAAATTCG AAAACATTTC TCAACTACCC 
CAAGCTCCAG CCCGTAGCGT TAGTAGCAGA GAGGCGGTGC AGAGGGGCCT TAACTCGTGG 
CAGCAAAGTG GAAGCAGGCC CCCATCCCGG GATGGAACTA CTGAGAATCA AGTTGCCAGT 
CCTAGCCCGC AGCCAAGCAC TTTCCAGACT GTTCCACAGA GCCACAGCAT CCCAGAATTC 
CCCAAAATGG CTTCCCAGAA GGAGATTGAG CAGTCTATAG AAGCACTTAA TATGCTTATG 
CTGGACCTGG ACCCTTCATT CTCACAAATG CACAAGTCTC AGAGTGTGCC TGTTGCCCCT 
AGAGAAGAAA GGGTTCTGCC AGTGCAAGGA TCCTTCTCAG CGCAGCCATC TTTGGGCATT 
TATAGCCAGC CGACTCCACA GGTGGCTACA CAAAGGCCTA GCAACGCTTA CGTGGCTCCT 
AGCATGGTGC AGAGCGCTGG AGCCTCTCAA AGCCAACCAC ACTTGCCTTC GGAACAGGTT 
TCGAGAACAT ATTCACCTGG GGTGCATCAT GACCAAGCTC GGGCTGTTGG GCCAGAAAAA 
GTTCTGAATG ACTATCGGGA GCCTTATGCT GCTGGAACCT TCTCTCCTTA TGGTTATCAG 
AACCAACAAC CGCCTTTGGC ATTGACAAGA AGCTATAGCT ATGGAAGTAG TGCTTCGCCA 
TCTTCGGTTC CACCATTATC GGCATATATG CAGAGCAATA CTCAGCAACC CGTCCTTTCT 
TCTCCTGCTT CTTCTGTGGT CATGGCTCAA CCTCAGCCTC AATCCCAGTC TCCGACCAGA 
GAGCCAGACG ATGAACAGTA CAGTCTGGAA GGGCTGGTAG CTCACAGAAT TGCTGGGGTG 
CACACTCGGG ACAAGACTCC GGATGAGATC GGGTCCCTGG CACGGAGGCG CACCACCAGC 
GAGGGGCAAT ATGACGAGGG CAATGCTGAC CTGGGCAGAG CGAGTAGCCC CACCAAGGTG 
CGCTCCCCAG TGCGCTGCGT CTCCCCGGAA GTCGCCTTCA CCATTGCGGC AAACCCAGGG 
GGGAGACCCA AGGAGCCCCA TCTGCACAGC TACAAGGAGG CCTTTGAAGA GATGGAAATG 
ACGCCGCCAG TGATCAGCCC GGGGGCCACA GTAGCCCGAT CCCCCCCTGG TTTAGCCAAG 
ACCCCCCTGT CTGCCCTGGG ACTGAAGCCC CACAATCCCG CAGAAATCGT TCTCCAGCAA 
TCGGGAGAGC CCAGGAGTTA TGTTGAATCC GTTGCCCGGA CGGCTGCCGC GGGAGGAGTC 
GCACCCCCAT CACCTACAGG CCCCCAGGCA TATAGTGTCG ATTCTCTGAT GAGAAATGGG 
GGTTACCCCA ATGTTTTCAC CTCTCCCAGC CCCATTTCTA CCAGCAGCCC CATACACAGC 
ACTGATGGCT CCCTAAGGAG TTACCCGTCG GTGGAGAGCG GCGCCCGTGT CCCCACTCCC 
ACTCAGCCTT CCGTGGATTC CAGCTTCAGA TCCACCAGTC TATCGCAGCC GTCGCCCCAA 
AGCAGTTACC AGAACGCCTC TCCCATGGTT CCCTACGCTG GCACACCGGC CAGTTCCTAT 
GATGGATACC CTTATTCCCC CAGCAGTGCC CTCACCCAGA CAGACGGCCT CTCCGGGCCA 
CAGATGAACG CGGTCATTGG TCACATGGTT CACGGAAGCC CCAAGGCCGT TCATAGAACG 
GTGGGGACCA ACACACCACC GAGTCCAGGT TTTGGGAGGA GAATGGTCAC CCCCAATATG 
GCGCCTCCAC CCGGAAGTCC AAGCATGGGC AGACACCCCA TGGCTACACA TATGGCTGCA 
CCCGTTACAG TAGGTAGCCC CAGCCTTCCT CTCCACCAGG CCTTGATGAC GGGAAATGCT 
CCGGTGACTC CTGGTAGCCC CGGCTTGGAG AGACACGTTA TGTATGGCTA TTCCCCCGCT 
GATGACAAAC GACCGACACT GTCACGGCAG AGCAGTGCGT CCGGATACCA GGCACCGCCC 
ACCCCATCCT TCCCGGTCTC CCCGGCCTAT TACCATGGCA TGAGTAGCCC TTCCTCCTCC 
TCTCCTGACT CAACCTTATA CCGGCAAGGG AGCCCAATCT CACAGCCGTC TCTGCCCGAG 
AAGAGGAGAA TGTCATCTGG GGACAGATCC AACAGCCTCC CCAACTACTC CACCCTCAAT 
GGAAAAACCT CTTCGCCCAT GTCCAGCGGG ATGTCCAGCC CCAGCGGCGG AAGCACAATG 
GCATTTACTC ACACTCTACC GGACTTCTCC AAGTTCTCCA TGCCAGACAG CAGCCCGGAG 
ACCCGAGCAA ATGTGAAGTT TGTGCAGGAC ACGTCCAAGT ACTGGTACAA ACCAGACATT 
TCCAGGGATC AGGCCATCTC CCTGCTGAAG GACAAAGACC CCGGGGCGTT CATCATACGA 
GACAGCCATT CGTTCCGAGG GGCCTACGGA CTTGCCATGA AAGTTGCCAC CCCGCCCCCC 
AACGCTGGCC AGCCCAGCAA GAAGGGAGAT GTGACCAATG AGCTGGTTCG GCACTTTTTG 
ATTGAGACGA GTCCCAGGGG GGTGAAATTA AAGGGCTGCC AGAACGAACC CTACTTTGGC 
TGCCTGTCGG CTCTGGTGTA CCAGCACTCC ATTACTCCCC TTGCACTGCC CTGCAAACTG 
GTCATACCCT GCAGAGATCC CTCTGATGAG ACCAAGGAGA CGGCTGTTCC GGCCAATTCA 
GCCTCAGATT TACTGAAACA GGGCGCAGCC TGCAACGTGT TATTCATAAA CTCGATAGAC 
ATGGAGTCGC TGACCGGCCC ACAAGCCATT GCGAAGACCA TCGCCGAGTC TCTCGCCGCC 
GACCCCCCGC CGGCCGCCGC CATCGTTCAC TTCAAAGTCT CCGCCCAAGG AATCACCTTA 
ACCGACAACC AGAGAAAATT ATTTTTCAGA CGACATTATC CGCTCAGCTC AGTCACCTTC 
TGCGACCTGG ACCCGCAAGA GAGAAAGTGG ATGAAGGCCG ATGGGACACA AGCCAAACTC 
TTTGGCTTTG TGGCCAGAAA GCAAGGAAGC ACCACAGACA ATATGTGCCA CCTGTTTGCC 
GAACTTGACC CAAACCAACC AGCCACTGCC ATCGTCAACT TTGTGTCCCG AGTCATCGGC 
CTGCAGAAGA GATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031749633.1
CDS	246..5720
Translation	MGCTVSFICCEEDPPLMSAEPQHEAETKEEKKRLLPKPEDLEGQNLHTFKVKTFKKVKSCSVCKQAITREGSACRVCRLSCHRKCEAKIASPCTPRVNYELPSSSESPQKNVETTGSTKSSRSTQSRRRPSRSISLIHAMEDSCELDLTYITERIISVTFSTGTEEPTFCRNLKEVAHMLKSKHGDNYLIFNLSERRHDISKLHSKVLDFGWPDLHAPALEKVCSICKAMDTWLNADPHNVVVIHNKGNRGRTGVVIAAYMHYSNISASADQALDRFAMRRFYEDKVLPVAQPSQKRYINYFSGLLSGAMKMNSKPLFLHHVIMHGIPNFESKGGCRPFLKIYQAMQPVYTSGIYNVQGDSQTSICITIEPGLLLKGDILLKCYHKKFRSPTRDVIFRVQFHTCAIHDLGIVFGKEDLDEAFKDERFPEYGKVEFVFSYGPEKIQGMEHLENGPSVSVDYNTSDPLIRWDSYDNFNVHREDSLEEVGHTQGPLDGSLYAKVKKKDSTNGSTSTVNASGIPLSAAPNHIEHTLSVSSDSGNSTASTKTDKTDEQVATAAANQGLTPEEKQELERLLSGFGLETEGQMHNLNPGTSAAAAMLHVVPAQVHVNGERTSPQVERETDILDDDLPNHDVHSVGSLGTLSSCDVIPSLSETGYQDSHLRMSPLTNGPATVNGIDNLQKHGFIGKETMINGGYPYNNQNVLKSALIPHSQDAGIHMRPSVSAHENLAYQVNQPNWGQPSPSNVTQKHLYYYDPNGMYRSQSYTAAEPGKFENISQLPQAPARSVSSREAVQRGLNSWQQSGSRPPSRDGTTENQVASPSPQPSTFQTVPQSHSIPEFPKMASQKEIEQSIEALNMLMLDLDPSFSQMHKSQSVPVAPREERVLPVQGSFSAQPSLGIYSQPTPQVATQRPSNAYVAPSMVQSAGASQSQPHLPSEQVSRTYSPGVHHDQARAVGPEKVLNDYREPYAAGTFSPYGYQNQQPPLALTRSYSYGSSASPSSVPPLSAYMQSNTQQPVLSSPASSVVMAQPQPQSQSPTREPDDEQYSLEGLVAHRIAGVHTRDKTPDEIGSLARRRTTSEGQYDEGNADLGRASSPTKVRSPVRCVSPEVAFTIAANPGGRPKEPHLHSYKEAFEEMEMTPPVISPGATVARSPPGLAKTPLSALGLKPHNPAEIVLQQSGEPRSYVESVARTAAAGGVAPPSPTGPQAYSVDSLMRNGGYPNVFTSPSPISTSSPIHSTDGSLRSYPSVESGARVPTPTQPSVDSSFRSTSLSQPSPQSSYQNASPMVPYAGTPASSYDGYPYSPSSALTQTDGLSGPQMNAVIGHMVHGSPKAVHRTVGTNTPPSPGFGRRMVTPNMAPPPGSPSMGRHPMATHMAAPVTVGSPSLPLHQALMTGNAPVTPGSPGLERHVMYGYSPADDKRPTLSRQSSASGYQAPPTPSFPVSPAYYHGMSSPSSSSPDSTLYRQGSPISQPSLPEKRRMSSGDRSNSLPNYSTLNGKTSSPMSSGMSSPSGGSTMAFTHTLPDFSKFSMPDSSPETRANVKFVQDTSKYWYKPDISRDQAISLLKDKDPGAFIIRDSHSFRGAYGLAMKVATPPPNAGQPSKKGDVTNELVRHFLIETSPRGVKLKGCQNEPYFGCLSALVYQHSITPLALPCKLVIPCRDPSDETKETAVPANSASDLLKQGAACNVLFINSIDMESLTGPQAIAKTIAESLAADPPPAAAIVHFKVSAQGITLTDNQRKLFFRRHYPLSSVTFCDLDPQERKWMKADGTQAKLFGFVARKQGSTTDNMCHLFAELDPNQPATAIVNFVSRVIGLQKR

Target ORF information:

RefSeq Version	XM_031893773.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis tensin 1 (tns1), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031893773.1

ORF Insert Sequence:

ATGGGATGCA CCGTCAGCTT CATCTGCTGT GAAGAGGACC CCCCGCTCAT GTCTGCTGAG 
CCCCAACATG AGGCCGAAAC GAAGGAGGAA AAAAAGAGGC TCCTTCCCAA GCCTGAAGAC 
CTGGAAGGGC AGAACCTTCA TACATTCAAG GTGAAGACCT TTAAGAAGGT GAAATCGTGC 
AGCGTGTGCA AGCAGGCCAT CACCAGAGAG GGCAGTGCGT GCCGGGTTTG CCGATTGTCG 
TGCCACAGAA AATGTGAAGC CAAGATTGCG TCGCCCTGTA CTCCTCGGGT CAATTATGAG 
CTGCCGTCAA GCTCTGAAAG CCCCCAGAAG AATGTAGAAA CCACAGGATC CACCAAATCC 
TCCAGAAGCA CTCAGTCCCG CCGCAGACCA TCAAGGAGCA TTAGCCTGAT TCACGCCATG 
GAGGACAGCT GCGAGTTGGA CCTCACCTAC ATCACCGAGC GGATCATCTC CGTCACCTTC 
TCCACCGGGA CGGAAGAGCC CACCTTCTGC CGGAACCTGA AGGAAGTGGC GCATATGCTG 
AAGTCCAAAC ATGGAGACAA TTACCTGATC TTCAACTTGT CGGAACGCAG ACATGACATC 
AGCAAGCTCC ATTCCAAGGT GTTGGATTTC GGATGGCCGG ACCTGCACGC GCCGGCCTTG 
GAAAAAGTCT GCAGCATCTG CAAAGCCATG GACACCTGGC TCAACGCCGA CCCTCACAAC 
GTCGTCGTCA TCCATAACAA AGGCAACCGA GGGCGGACAG GTGTGGTGAT TGCCGCCTAT 
ATGCACTACA GCAACATATC TGCCAGTGCA GACCAAGCCT TGGACAGGTT TGCCATGAGA 
AGGTTCTATG AAGACAAAGT CCTTCCAGTA GCTCAACCTT CCCAAAAAAG GTATATAAAC 
TACTTCAGTG GGCTTCTCTC TGGCGCCATG AAAATGAACA GCAAACCTCT CTTCCTGCAT 
CATGTGATCA TGCATGGGAT CCCCAACTTT GAGTCCAAGG GAGGTTGTCG GCCATTTCTG 
AAGATCTACC AAGCGATGCA GCCTGTATAC ACCTCGGGCA TCTACAACGT GCAAGGGGAC 
AGCCAGACGA GCATCTGCAT CACCATAGAA CCGGGGCTGT TACTAAAAGG TGACATCTTG 
CTCAAGTGCT ACCACAAGAA GTTCCGCAGC CCCACGCGGG ACGTCATATT CCGGGTGCAG 
TTCCATACGT GCGCCATCCA TGATCTGGGA ATCGTGTTTG GGAAGGAGGA TCTGGACGAA 
GCTTTCAAAG ATGAGCGTTT TCCCGAATAT GGAAAAGTGG AATTTGTTTT TTCATATGGA 
CCAGAAAAGA TACAAGGCAT GGAGCACCTG GAGAATGGAC CCAGCGTCTC TGTGGATTAT 
AACACATCCG ACCCTCTGAT TCGCTGGGAC TCCTATGATA ACTTCAACGT GCACCGGGAA 
GACAGCCTGG AAGAGGTTGG ACACACTCAA GGCCCCTTGG ATGGAAGCCT GTACGCCAAG 
GTGAAGAAGA AAGACTCTAC GAACGGCAGC ACCAGCACCG TCAACGCCAG CGGAATTCCG 
TTGTCTGCCG CGCCCAATCA CATTGAACAC ACCCTGTCCG TGAGCAGCGA CTCCGGCAAC 
TCGACTGCTT CGACCAAAAC CGACAAGACC GATGAGCAAG TGGCCACGGC GGCTGCCAAC 
CAAGGGTTGA CCCCTGAGGA GAAGCAAGAG TTGGAGCGGC TCCTGAGCGG CTTTGGCTTG 
GAGACCGAAG GGCAGATGCA CAATTTAAAT CCGGGCACAT CTGCAGCAGC AGCGATGCTT 
CACGTTGTGC CGGCGCAAGT CCACGTCAAT GGGGAACGGA CATCTCCGCA GGTGGAACGA 
GAGACAGATA TTCTTGATGA TGATCTGCCC AACCATGATG TTCACAGTGT TGGAAGCTTG 
GGGACCCTGT CTTCGTGTGA TGTGATTCCA AGCTTGAGTG AGACTGGCTA CCAGGATTCT 
CACCTTCGCA TGTCCCCCTT GACCAATGGT CCAGCCACTG TTAATGGCAT TGATAACCTG 
CAAAAACATG GGTTTATTGG TAAAGAGACG ATGATTAATG GCGGCTACCC GTACAACAAC 
CAGAATGTCT TAAAAAGCGC TTTGATCCCT CATTCCCAGG ATGCAGGGAT CCATATGCGA 
CCTTCCGTGT CTGCTCATGA AAACTTAGCC TATCAAGTCA ACCAGCCCAA CTGGGGACAA 
CCTTCTCCAT CAAATGTAAC ACAAAAGCAC TTATACTATT ATGACCCGAA TGGCATGTAC 
CGTTCACAGT CGTACACCGC TGCCGAACCA GGGAAATTCG AAAACATTTC TCAACTACCC 
CAAGCTCCAG CCCGTAGCGT TAGTAGCAGA GAGGCGGTGC AGAGGGGCCT TAACTCGTGG 
CAGCAAAGTG GAAGCAGGCC CCCATCCCGG GATGGAACTA CTGAGAATCA AGTTGCCAGT 
CCTAGCCCGC AGCCAAGCAC TTTCCAGACT GTTCCACAGA GCCACAGCAT CCCAGAATTC 
CCCAAAATGG CTTCCCAGAA GGAGATTGAG CAGTCTATAG AAGCACTTAA TATGCTTATG 
CTGGACCTGG ACCCTTCATT CTCACAAATG CACAAGTCTC AGAGTGTGCC TGTTGCCCCT 
AGAGAAGAAA GGGTTCTGCC AGTGCAAGGA TCCTTCTCAG CGCAGCCATC TTTGGGCATT 
TATAGCCAGC CGACTCCACA GGTGGCTACA CAAAGGCCTA GCAACGCTTA CGTGGCTCCT 
AGCATGGTGC AGAGCGCTGG AGCCTCTCAA AGCCAACCAC ACTTGCCTTC GGAACAGGTT 
TCGAGAACAT ATTCACCTGG GGTGCATCAT GACCAAGCTC GGGCTGTTGG GCCAGAAAAA 
GTTCTGAATG ACTATCGGGA GCCTTATGCT GCTGGAACCT TCTCTCCTTA TGGTTATCAG 
AACCAACAAC CGCCTTTGGC ATTGACAAGA AGCTATAGCT ATGGAAGTAG TGCTTCGCCA 
TCTTCGGTTC CACCATTATC GGCATATATG CAGAGCAATA CTCAGCAACC CGTCCTTTCT 
TCTCCTGCTT CTTCTGTGGT CATGGCTCAA CCTCAGCCTC AATCCCAGTC TCCGACCAGA 
GAGCCAGACG ATGAACAGTA CAGTCTGGAA GGGCTGGTAG CTCACAGAAT TGCTGGGGTG 
CACACTCGGG ACAAGACTCC GGATGAGATC GGGTCCCTGG CACGGAGGCG CACCACCAGC 
GAGGGGCAAT ATGACGAGGG CAATGCTGAC CTGGGCAGAG CGAGTAGCCC CACCAAGGTG 
CGCTCCCCAG TGCGCTGCGT CTCCCCGGAA GTCGCCTTCA CCATTGCGGC AAACCCAGGG 
GGGAGACCCA AGGAGCCCCA TCTGCACAGC TACAAGGAGG CCTTTGAAGA GATGGAAATG 
ACGCCGCCAG TGATCAGCCC GGGGGCCACA GTAGCCCGAT CCCCCCCTGG TTTAGCCAAG 
ACCCCCCTGT CTGCCCTGGG ACTGAAGCCC CACAATCCCG CAGAAATCGT TCTCCAGCAA 
TCGGGAGAGC CCAGGAGTTA TGTTGAATCC GTTGCCCGGA CGGCTGCCGC GGGAGGAGTC 
GCACCCCCAT CACCTACAGG CCCCCAGGCA TATAGTGTCG ATTCTCTGAT GAGAAATGGG 
GGTTACCCCA ATGTTTTCAC CTCTCCCAGC CCCATTTCTA CCAGCAGCCC CATACACAGC 
ACTGATGGCT CCCTAAGGAG TTACCCGTCG GTGGAGAGCG GCGCCCGTGT CCCCACTCCC 
ACTCAGCCTT CCGTGGATTC CAGCTTCAGA TCCACCAGTC TATCGCAGCC GTCGCCCCAA 
AGCAGTTACC AGAACGCCTC TCCCATGGTT CCCTACGCTG GCACACCGGC CAGTTCCTAT 
GATGGATACC CTTATTCCCC CAGCAGTGCC CTCACCCAGA CAGACGGCCT CTCCGGGCCA 
CAGATGAACG CGGTCATTGG TCACATGGTT CACGGAAGCC CCAAGGCCGT TCATAGAACG 
GTGGGGACCA ACACACCACC GAGTCCAGGT TTTGGGAGGA GAATGGTCAC CCCCAATATG 
GCGCCTCCAC CCGGAAGTCC AAGCATGGGC AGACACCCCA TGGCTACACA TATGGCTGCA 
CCCGTTACAG TAGGTAGCCC CAGCCTTCCT CTCCACCAGG CCTTGATGAC GGGAAATGCT 
CCGGTGACTC CTGGTAGCCC CGGCTTGGAG AGACACGTTA TGTATGGCTA TTCCCCCGCT 
GATGACAAAC GACCGACACT GTCACGGCAG AGCAGTGCGT CCGGATACCA GGCACCGCCC 
ACCCCATCCT TCCCGGTCTC CCCGGCCTAT TACCATGGCA TGAGTAGCCC TTCCTCCTCC 
TCTCCTGACT CAACCTTATA CCGGCAAGGG AGCCCAATCT CACAGCCGTC TCTGCCCGAG 
AAGAGGAGAA TGTCATCTGG GGACAGATCC AACAGCCTCC CCAACTACTC CACCCTCAAT 
GGAAAAACCT CTTCGCCCAT GTCCAGCGGG ATGTCCAGCC CCAGCGGCGG AAGCACAATG 
GCATTTACTC ACACTCTACC GGACTTCTCC AAGTTCTCCA TGCCAGACAG CAGCCCGGAG 
ACCCGAGCAA ATGTGAAGTT TGTGCAGGAC ACGTCCAAGT ACTGGTACAA ACCAGACATT 
TCCAGGGATC AGGCCATCTC CCTGCTGAAG GACAAAGACC CCGGGGCGTT CATCATACGA 
GACAGCCATT CGTTCCGAGG GGCCTACGGA CTTGCCATGA AAGTTGCCAC CCCGCCCCCC 
AACGCTGGCC AGCCCAGCAA GAAGGGAGAT GTGACCAATG AGCTGGTTCG GCACTTTTTG 
ATTGAGACGA GTCCCAGGGG GGTGAAATTA AAGGGCTGCC AGAACGAACC CTACTTTGGC 
TGCCTGTCGG CTCTGGTGTA CCAGCACTCC ATTACTCCCC TTGCACTGCC CTGCAAACTG 
GTCATACCCT GCAGAGATCC CTCTGATGAG ACCAAGGAGA CGGCTGTTCC GGCCAATTCA 
GCCTCAGATT TACTGAAACA GGGCGCAGCC TGCAACGTGT TATTCATAAA CTCGATAGAC 
ATGGAGTCGC TGACCGGCCC ACAAGCCATT GCGAAGACCA TCGCCGAGTC TCTCGCCGCC 
GACCCCCCGC CGGCCGCCGC CATCGTTCAC TTCAAAGTCT CCGCCCAAGG AATCACCTTA 
ACCGACAACC AGAGAAAATT ATTTTTCAGA CGACATTATC CGCTCAGCTC AGTCACCTTC 
TGCGACCTGG ACCCGCAAGA GAGAAAGTGG ATGAAGGCCG ATGGGACACA AGCCAAACTC 
TTTGGCTTTG TGGCCAGAAA GCAAGGAAGC ACCACAGACA ATATGTGCCA CCTGTTTGCC 
GAACTTGACC CAAACCAACC AGCCACTGCC ATCGTCAACT TTGTGTCCCG AGTCATCGGC 
CTGCAGAAGA GATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

tns1 ( XM_031893774.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_031749634.1 Xenopus tropicalis tensin 1 (tns1), transcript variant X6, mRNA.

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CloneID	OXa67406
Clone ID Related Accession (Same CDS sequence)	XM_031893774.1
Accession Version	XM_031893774.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5184bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1576512000000
Organism	Xenopus tropicalis(tropical clawed frog)
Product	tensin-1 isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030685.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGACA GCTGCGAGTT GGACCTCACC TACATCACCG AGCGGATCAT CTCCGTCACC 
TTCTCCACCG GGACGGAAGA GCCCACCTTC TGCCGGAACC TGAAGGAAGT GGCGCATATG 
CTGAAGTCCA AACATGGAGA CAATTACCTG ATCTTCAACT TGTCGGAACG CAGACATGAC 
ATCAGCAAGC TCCATTCCAA GGTGTTGGAT TTCGGATGGC CGGACCTGCA CGCGCCGGCC 
TTGGAAAAAG TCTGCAGCAT CTGCAAAGCC ATGGACACCT GGCTCAACGC CGACCCTCAC 
AACGTCGTCG TCATCCATAA CAAAGGCAAC CGAGGGCGGA CAGGTGTGGT GATTGCCGCC 
TATATGCACT ACAGCAACAT ATCTGCCAGT GCAGACCAAG CCTTGGACAG GTTTGCCATG 
AGAAGGTTCT ATGAAGACAA AGTCCTTCCA GTAGCTCAAC CTTCCCAAAA AAGGTATATA 
AACTACTTCA GTGGGCTTCT CTCTGGCGCC ATGAAAATGA ACAGCAAACC TCTCTTCCTG 
CATCATGTGA TCATGCATGG GATCCCCAAC TTTGAGTCCA AGGGAGGTTG TCGGCCATTT 
CTGAAGATCT ACCAAGCGAT GCAGCCTGTA TACACCTCGG GCATCTACAA CGTGCAAGGG 
GACAGCCAGA CGAGCATCTG CATCACCATA GAACCGGGGC TGTTACTAAA AGGTGACATC 
TTGCTCAAGT GCTACCACAA GAAGTTCCGC AGCCCCACGC GGGACGTCAT ATTCCGGGTG 
CAGTTCCATA CGTGCGCCAT CCATGATCTG GGAATCGTGT TTGGGAAGGA GGATCTGGAC 
GAAGCTTTCA AAGATGAGCG TTTTCCCGAA TATGGAAAAG TGGAATTTGT TTTTTCATAT 
GGACCAGAAA AGATACAAGG CATGGAGCAC CTGGAGAATG GACCCAGCGT CTCTGTGGAT 
TATAACACAT CCGACCCTCT GATTCGCTGG GACTCCTATG ATAACTTCAA CGTGCACCGG 
GAAGACAGCC TGGAAGAGGT TGGACACACT CAAGGCCCCT TGGATGGAAG CCTGTACGCC 
AAGGTGAAGA AGAAAGACTC TACGAACGGC AGCACCAGCA CCGTCAACGC CAGCGGAATT 
CCGTTGTCTG CCGCGCCCAA TCACATTGAA CACACCCTGT CCGTGAGCAG CGACTCCGGC 
AACTCGACTG CTTCGACCAA AACCGACAAG ACCGATGAGC AAGTGGCCAC GGCGGCTGCC 
AACCAAGGGT TGACCCCTGA GGAGAAGCAA GAGTTGGAGC GGCTCCTGAG CGGCTTTGGC 
TTGGAGACCG AAGGGCAGAT GCACAATTTA AATCCGGGCA CATCTGCAGC AGCAGCGATG 
CTTCACGTTG TGCCGGCGCA AGTCCACGTC AATGGGGAAC GGACATCTCC GCAGGTGGAA 
CGAGAGACAG ATATTCTTGA TGATGATCTG CCCAACCATG ATGTTCACAG TGTTGGAAGC 
TTGGGGACCC TGTCTTCGTG TGATGTGATT CCAAGCTTGA GTGAGACTGG CTACCAGGAT 
TCTCACCTTC GCATGTCCCC CTTGACCAAT GGTCCAGCCA CTGTTAATGG CATTGATAAC 
CTGCAAAAAC ATGGGTTTAT TGGTAAAGAG ACGATGATTA ATGGCGGCTA CCCGTACAAC 
AACCAGAATG TCTTAAAAAG CGCTTTGATC CCTCATTCCC AGGATGCAGG GATCCATATG 
CGACCTTCCG TGTCTGCTCA TGAAAACTTA GCCTATCAAG TCAACCAGCC CAACTGGGGA 
CAACCTTCTC CATCAAATGT AACACAAAAG CACTTATACT ATTATGACCC GAATGGCATG 
TACCGTTCAC AGTCGTACAC CGCTGCCGAA CCAGGGAAAT TCGAAAACAT TTCTCAACTA 
CCCCAAGCTC CAGCCCGTAG CGTTAGTAGC AGAGAGGCGG TGCAGAGGGG CCTTAACTCG 
TGGCAGCAAA GTGGAAGCAG GCCCCCATCC CGGGATGGAA CTACTGAGAA TCAAGTTGCC 
AGTCCTAGCC CGCAGCCAAG CACTTTCCAG ACTGTTCCAC AGAGCCACAG CATCCCAGAA 
TTCCCCAAAA TGGCTTCCCA GAAGGAGATT GAGCAGTCTA TAGAAGCACT TAATATGCTT 
ATGCTGGACC TGGACCCTTC ATTCTCACAA ATGCACAAGT CTCAGAGTGT GCCTGTTGCC 
CCTAGAGAAG AAAGGGTTCT GCCAGTGCAA GGATCCTTCT CAGCGCAGCC ATCTTTGGGC 
ATTTATAGCC AGCCGACTCC ACAGGTGGCT ACACAAAGGC CTAGCAACGC TTACGTGGCT 
CCTAGCATGG TGCAGAGCGC TGGAGCCTCT CAAAGCCAAC CACACTTGCC TTCGGAACAG 
GTTTCGAGAA CATATTCACC TGGGGTGCAT CATGACCAAG CTCGGGCTGT TGGGCCAGAA 
AAAGTTCTGA ATGACTATCG GGAGCCTTAT GCTGCTGGAA CCTTCTCTCC TTATGGTTAT 
CAGAACCAAC AACCGCCTTT GGCATTGACA AGAAGCTATA GCTATGGAAG TAGTGCTTCG 
CCATCTTCGG TTCCACCATT ATCGGCATAT ATGCAGAGCA ATACTCAGCA ACCCGTCCTT 
TCTTCTCCTG CTTCTTCTGT GGTCATGGCT CAACCTCAGC CTCAATCCCA GTCTCCGACC 
AGAGAGCCAG ACGATGAACA GTACAGTCTG GAAGGGCTGG TAGCTCACAG AATTGCTGAG 
TACAACAACA AACTCCGGAA TATCCGTAAA AGCATGAATA GCCCCCATCC CCATGGCGTG 
CAGCGCCAGC GTTCCTTCAC TTTCTCTGGG GTGCACACTC GGGACAAGAC TCCGGATGAG 
ATCGGGTCCC TGGCACGGAG GCGCACCACC AGCGAGGGGC AATATGACGA GGGCAATGCT 
GACCTGGGCA GAGCGAGTAG CCCCACCAAG GTGCGCTCCC CAGTGCGCTG CGTCTCCCCG 
GAAGTCGCCT TCACCATTGC GGCAAACCCA GGGGGGAGAC CCAAGGAGCC CCATCTGCAC 
AGCTACAAGG AGGCCTTTGA AGAGATGGAA ATGACGCCGC CAGTGATCAG CCCGGGGGCC 
ACAGTAGCCC GATCCCCCCC TGGTTTAGCC AAGACCCCCC TGTCTGCCCT GGGACTGAAG 
CCCCACAATC CCGCAGAAAT CGTTCTCCAG CAATCGGGAG AGACAGTCAG GGAAACAGTA 
ATTGCTTACA TGGAAGAGCC CAGGAGTTAT GTTGAATCCG TTGCCCGGAC GGCTGCCGCG 
GGAGGAGTCG CACCCCCATC ACCTACAGGC CCCCAGGCAT ATAGTGTCGA TTCTCTGATG 
AGAAATGGGG GTTACCCCAA TGTTTTCACC TCTCCCAGCC CCATTTCTAC CAGCAGCCCC 
ATACACAGCA CTGATGGCTC CCTAAGGAGT TACCCGTCGG TGGAGAGCGG CGCCCGTGTC 
CCCACTCCCA CTCAGCCTTC CGTGGATTCC AGCTTCAGAT CCACCAGTCT ATCGCAGCCG 
TCGCCCCAAA GCAGTTACCA GAACGCCTCT CCCATGGTTC CCTACGCTGG CACACCGGCC 
AGTTCCTATG ATGGATACCC TTATTCCCCC AGCAGTGCCC TCACCCAGAC AGACGGCCTC 
TCCGGGCCAC AGATGAACGC GGTCATTGGT CACATGGTTC ACGGAAGCCC CAAGGCCGTT 
CATAGAACGG TGGGGACCAA CACACCACCG AGTCCAGGTT TTGGGAGGAG AATGGTCACC 
CCCAATATGG CGCCTCCACC CGGAAGTCCA AGCATGGGCA GACACCCCAT GGCTACACAT 
ATGGCTGCAC CCGTTACAGT AGGTAGCCCC AGCCTTCCTC TCCACCAGGC CTTGATGACG 
GGAAATGCTC CGGTGACTCC TGGTAGCCCC GGCTTGGAGA GACACGTTAT GTATGGCTAT 
TCCCCCGCTG ATGACAAACG ACCGACACTG TCACGGCAGA GCAGTGCGTC CGGATACCAG 
GCACCGCCCA CCCCATCCTT CCCGGTCTCC CCGGCCTATT ACCATGGCAT GAGTAGCCCT 
TCCTCCTCCT CTCCTGACTC AACCTTATAC CGGCAAGGGA GCCCAATCTC ACAGCCGTCT 
CTGCCCGAGA AGAGGAGAAT GTCATCTGGG GACAGATCCA ACAGCCTCCC CAACTACTCC 
ACCCTCAATG GAAAAACCTC TTCGCCCATG TCCAGCGGGA TGTCCAGCCC CAGCGGCGGA 
AGCACAATGG CATTTACTCA CACTCTACCG GACTTCTCCA AGTTCTCCAT GCCAGACAGC 
AGCCCGGAGA CCCGAGCAAA TGTGAAGTTT GTGCAGGACA CGTCCAAGTA CTGGTACAAA 
CCAGACATTT CCAGGGATCA GGCCATCTCC CTGCTGAAGG ACAAAGACCC CGGGGCGTTC 
ATCATACGAG ACAGCCATTC GTTCCGAGGG GCCTACGGAC TTGCCATGAA AGTTGCCACC 
CCGCCCCCCA ACGCTGGCCA GCCCAGCAAG AAGGGAGATG TGACCAATGA GCTGGTTCGG 
CACTTTTTGA TTGAGACGAG TCCCAGGGGG GTGAAATTAA AGGGCTGCCA GAACGAACCC 
TACTTTGGCT GCCTGTCGGC TCTGGTGTAC CAGCACTCCA TTACTCCCCT TGCACTGCCC 
TGCAAACTGG TCATACCCTG CAGAGATCCC TCTGATGAGA CCAAGGAGAC GGCTGTTCCG 
GCCAATTCAG CCTCAGATTT ACTGAAACAG GGCGCAGCCT GCAACGTGTT ATTCATAAAC 
TCGATAGACA TGGAGTCGCT GACCGGCCCA CAAGCCATTG CGAAGACCAT CGCCGAGTCT 
CTCGCCGCCG ACCCCCCGCC GGCCGCCGCC ATCGTTCACT TCAAAGTCTC CGCCCAAGGA 
ATCACCTTAA CCGACAACCA GAGAAAATTA TTTTTCAGAC GACATTATCC GCTCAGCTCA 
GTCACCTTCT GCGACCTGGA CCCGCAAGAG AGAAAGTGGA TGAAGGCCGA TGGGACACAA 
GCCAAACTCT TTGGCTTTGT GGCCAGAAAG CAAGGAAGCA CCACAGACAA TATGTGCCAC 
CTGTTTGCCG AACTTGACCC AAACCAACCA GCCACTGCCA TCGTCAACTT TGTGTCCCGA 
GTCATCGGCC TGCAGAAGAG ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031749634.1
CDS	241..5424
Translation	MEDSCELDLTYITERIISVTFSTGTEEPTFCRNLKEVAHMLKSKHGDNYLIFNLSERRHDISKLHSKVLDFGWPDLHAPALEKVCSICKAMDTWLNADPHNVVVIHNKGNRGRTGVVIAAYMHYSNISASADQALDRFAMRRFYEDKVLPVAQPSQKRYINYFSGLLSGAMKMNSKPLFLHHVIMHGIPNFESKGGCRPFLKIYQAMQPVYTSGIYNVQGDSQTSICITIEPGLLLKGDILLKCYHKKFRSPTRDVIFRVQFHTCAIHDLGIVFGKEDLDEAFKDERFPEYGKVEFVFSYGPEKIQGMEHLENGPSVSVDYNTSDPLIRWDSYDNFNVHREDSLEEVGHTQGPLDGSLYAKVKKKDSTNGSTSTVNASGIPLSAAPNHIEHTLSVSSDSGNSTASTKTDKTDEQVATAAANQGLTPEEKQELERLLSGFGLETEGQMHNLNPGTSAAAAMLHVVPAQVHVNGERTSPQVERETDILDDDLPNHDVHSVGSLGTLSSCDVIPSLSETGYQDSHLRMSPLTNGPATVNGIDNLQKHGFIGKETMINGGYPYNNQNVLKSALIPHSQDAGIHMRPSVSAHENLAYQVNQPNWGQPSPSNVTQKHLYYYDPNGMYRSQSYTAAEPGKFENISQLPQAPARSVSSREAVQRGLNSWQQSGSRPPSRDGTTENQVASPSPQPSTFQTVPQSHSIPEFPKMASQKEIEQSIEALNMLMLDLDPSFSQMHKSQSVPVAPREERVLPVQGSFSAQPSLGIYSQPTPQVATQRPSNAYVAPSMVQSAGASQSQPHLPSEQVSRTYSPGVHHDQARAVGPEKVLNDYREPYAAGTFSPYGYQNQQPPLALTRSYSYGSSASPSSVPPLSAYMQSNTQQPVLSSPASSVVMAQPQPQSQSPTREPDDEQYSLEGLVAHRIAEYNNKLRNIRKSMNSPHPHGVQRQRSFTFSGVHTRDKTPDEIGSLARRRTTSEGQYDEGNADLGRASSPTKVRSPVRCVSPEVAFTIAANPGGRPKEPHLHSYKEAFEEMEMTPPVISPGATVARSPPGLAKTPLSALGLKPHNPAEIVLQQSGETVRETVIAYMEEPRSYVESVARTAAAGGVAPPSPTGPQAYSVDSLMRNGGYPNVFTSPSPISTSSPIHSTDGSLRSYPSVESGARVPTPTQPSVDSSFRSTSLSQPSPQSSYQNASPMVPYAGTPASSYDGYPYSPSSALTQTDGLSGPQMNAVIGHMVHGSPKAVHRTVGTNTPPSPGFGRRMVTPNMAPPPGSPSMGRHPMATHMAAPVTVGSPSLPLHQALMTGNAPVTPGSPGLERHVMYGYSPADDKRPTLSRQSSASGYQAPPTPSFPVSPAYYHGMSSPSSSSPDSTLYRQGSPISQPSLPEKRRMSSGDRSNSLPNYSTLNGKTSSPMSSGMSSPSGGSTMAFTHTLPDFSKFSMPDSSPETRANVKFVQDTSKYWYKPDISRDQAISLLKDKDPGAFIIRDSHSFRGAYGLAMKVATPPPNAGQPSKKGDVTNELVRHFLIETSPRGVKLKGCQNEPYFGCLSALVYQHSITPLALPCKLVIPCRDPSDETKETAVPANSASDLLKQGAACNVLFINSIDMESLTGPQAIAKTIAESLAADPPPAAAIVHFKVSAQGITLTDNQRKLFFRRHYPLSSVTFCDLDPQERKWMKADGTQAKLFGFVARKQGSTTDNMCHLFAELDPNQPATAIVNFVSRVIGLQKR

Target ORF information:

RefSeq Version	XM_031893774.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis tensin 1 (tns1), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031893774.1

ORF Insert Sequence:

ATGGAGGACA GCTGCGAGTT GGACCTCACC TACATCACCG AGCGGATCAT CTCCGTCACC 
TTCTCCACCG GGACGGAAGA GCCCACCTTC TGCCGGAACC TGAAGGAAGT GGCGCATATG 
CTGAAGTCCA AACATGGAGA CAATTACCTG ATCTTCAACT TGTCGGAACG CAGACATGAC 
ATCAGCAAGC TCCATTCCAA GGTGTTGGAT TTCGGATGGC CGGACCTGCA CGCGCCGGCC 
TTGGAAAAAG TCTGCAGCAT CTGCAAAGCC ATGGACACCT GGCTCAACGC CGACCCTCAC 
AACGTCGTCG TCATCCATAA CAAAGGCAAC CGAGGGCGGA CAGGTGTGGT GATTGCCGCC 
TATATGCACT ACAGCAACAT ATCTGCCAGT GCAGACCAAG CCTTGGACAG GTTTGCCATG 
AGAAGGTTCT ATGAAGACAA AGTCCTTCCA GTAGCTCAAC CTTCCCAAAA AAGGTATATA 
AACTACTTCA GTGGGCTTCT CTCTGGCGCC ATGAAAATGA ACAGCAAACC TCTCTTCCTG 
CATCATGTGA TCATGCATGG GATCCCCAAC TTTGAGTCCA AGGGAGGTTG TCGGCCATTT 
CTGAAGATCT ACCAAGCGAT GCAGCCTGTA TACACCTCGG GCATCTACAA CGTGCAAGGG 
GACAGCCAGA CGAGCATCTG CATCACCATA GAACCGGGGC TGTTACTAAA AGGTGACATC 
TTGCTCAAGT GCTACCACAA GAAGTTCCGC AGCCCCACGC GGGACGTCAT ATTCCGGGTG 
CAGTTCCATA CGTGCGCCAT CCATGATCTG GGAATCGTGT TTGGGAAGGA GGATCTGGAC 
GAAGCTTTCA AAGATGAGCG TTTTCCCGAA TATGGAAAAG TGGAATTTGT TTTTTCATAT 
GGACCAGAAA AGATACAAGG CATGGAGCAC CTGGAGAATG GACCCAGCGT CTCTGTGGAT 
TATAACACAT CCGACCCTCT GATTCGCTGG GACTCCTATG ATAACTTCAA CGTGCACCGG 
GAAGACAGCC TGGAAGAGGT TGGACACACT CAAGGCCCCT TGGATGGAAG CCTGTACGCC 
AAGGTGAAGA AGAAAGACTC TACGAACGGC AGCACCAGCA CCGTCAACGC CAGCGGAATT 
CCGTTGTCTG CCGCGCCCAA TCACATTGAA CACACCCTGT CCGTGAGCAG CGACTCCGGC 
AACTCGACTG CTTCGACCAA AACCGACAAG ACCGATGAGC AAGTGGCCAC GGCGGCTGCC 
AACCAAGGGT TGACCCCTGA GGAGAAGCAA GAGTTGGAGC GGCTCCTGAG CGGCTTTGGC 
TTGGAGACCG AAGGGCAGAT GCACAATTTA AATCCGGGCA CATCTGCAGC AGCAGCGATG 
CTTCACGTTG TGCCGGCGCA AGTCCACGTC AATGGGGAAC GGACATCTCC GCAGGTGGAA 
CGAGAGACAG ATATTCTTGA TGATGATCTG CCCAACCATG ATGTTCACAG TGTTGGAAGC 
TTGGGGACCC TGTCTTCGTG TGATGTGATT CCAAGCTTGA GTGAGACTGG CTACCAGGAT 
TCTCACCTTC GCATGTCCCC CTTGACCAAT GGTCCAGCCA CTGTTAATGG CATTGATAAC 
CTGCAAAAAC ATGGGTTTAT TGGTAAAGAG ACGATGATTA ATGGCGGCTA CCCGTACAAC 
AACCAGAATG TCTTAAAAAG CGCTTTGATC CCTCATTCCC AGGATGCAGG GATCCATATG 
CGACCTTCCG TGTCTGCTCA TGAAAACTTA GCCTATCAAG TCAACCAGCC CAACTGGGGA 
CAACCTTCTC CATCAAATGT AACACAAAAG CACTTATACT ATTATGACCC GAATGGCATG 
TACCGTTCAC AGTCGTACAC CGCTGCCGAA CCAGGGAAAT TCGAAAACAT TTCTCAACTA 
CCCCAAGCTC CAGCCCGTAG CGTTAGTAGC AGAGAGGCGG TGCAGAGGGG CCTTAACTCG 
TGGCAGCAAA GTGGAAGCAG GCCCCCATCC CGGGATGGAA CTACTGAGAA TCAAGTTGCC 
AGTCCTAGCC CGCAGCCAAG CACTTTCCAG ACTGTTCCAC AGAGCCACAG CATCCCAGAA 
TTCCCCAAAA TGGCTTCCCA GAAGGAGATT GAGCAGTCTA TAGAAGCACT TAATATGCTT 
ATGCTGGACC TGGACCCTTC ATTCTCACAA ATGCACAAGT CTCAGAGTGT GCCTGTTGCC 
CCTAGAGAAG AAAGGGTTCT GCCAGTGCAA GGATCCTTCT CAGCGCAGCC ATCTTTGGGC 
ATTTATAGCC AGCCGACTCC ACAGGTGGCT ACACAAAGGC CTAGCAACGC TTACGTGGCT 
CCTAGCATGG TGCAGAGCGC TGGAGCCTCT CAAAGCCAAC CACACTTGCC TTCGGAACAG 
GTTTCGAGAA CATATTCACC TGGGGTGCAT CATGACCAAG CTCGGGCTGT TGGGCCAGAA 
AAAGTTCTGA ATGACTATCG GGAGCCTTAT GCTGCTGGAA CCTTCTCTCC TTATGGTTAT 
CAGAACCAAC AACCGCCTTT GGCATTGACA AGAAGCTATA GCTATGGAAG TAGTGCTTCG 
CCATCTTCGG TTCCACCATT ATCGGCATAT ATGCAGAGCA ATACTCAGCA ACCCGTCCTT 
TCTTCTCCTG CTTCTTCTGT GGTCATGGCT CAACCTCAGC CTCAATCCCA GTCTCCGACC 
AGAGAGCCAG ACGATGAACA GTACAGTCTG GAAGGGCTGG TAGCTCACAG AATTGCTGAG 
TACAACAACA AACTCCGGAA TATCCGTAAA AGCATGAATA GCCCCCATCC CCATGGCGTG 
CAGCGCCAGC GTTCCTTCAC TTTCTCTGGG GTGCACACTC GGGACAAGAC TCCGGATGAG 
ATCGGGTCCC TGGCACGGAG GCGCACCACC AGCGAGGGGC AATATGACGA GGGCAATGCT 
GACCTGGGCA GAGCGAGTAG CCCCACCAAG GTGCGCTCCC CAGTGCGCTG CGTCTCCCCG 
GAAGTCGCCT TCACCATTGC GGCAAACCCA GGGGGGAGAC CCAAGGAGCC CCATCTGCAC 
AGCTACAAGG AGGCCTTTGA AGAGATGGAA ATGACGCCGC CAGTGATCAG CCCGGGGGCC 
ACAGTAGCCC GATCCCCCCC TGGTTTAGCC AAGACCCCCC TGTCTGCCCT GGGACTGAAG 
CCCCACAATC CCGCAGAAAT CGTTCTCCAG CAATCGGGAG AGACAGTCAG GGAAACAGTA 
ATTGCTTACA TGGAAGAGCC CAGGAGTTAT GTTGAATCCG TTGCCCGGAC GGCTGCCGCG 
GGAGGAGTCG CACCCCCATC ACCTACAGGC CCCCAGGCAT ATAGTGTCGA TTCTCTGATG 
AGAAATGGGG GTTACCCCAA TGTTTTCACC TCTCCCAGCC CCATTTCTAC CAGCAGCCCC 
ATACACAGCA CTGATGGCTC CCTAAGGAGT TACCCGTCGG TGGAGAGCGG CGCCCGTGTC 
CCCACTCCCA CTCAGCCTTC CGTGGATTCC AGCTTCAGAT CCACCAGTCT ATCGCAGCCG 
TCGCCCCAAA GCAGTTACCA GAACGCCTCT CCCATGGTTC CCTACGCTGG CACACCGGCC 
AGTTCCTATG ATGGATACCC TTATTCCCCC AGCAGTGCCC TCACCCAGAC AGACGGCCTC 
TCCGGGCCAC AGATGAACGC GGTCATTGGT CACATGGTTC ACGGAAGCCC CAAGGCCGTT 
CATAGAACGG TGGGGACCAA CACACCACCG AGTCCAGGTT TTGGGAGGAG AATGGTCACC 
CCCAATATGG CGCCTCCACC CGGAAGTCCA AGCATGGGCA GACACCCCAT GGCTACACAT 
ATGGCTGCAC CCGTTACAGT AGGTAGCCCC AGCCTTCCTC TCCACCAGGC CTTGATGACG 
GGAAATGCTC CGGTGACTCC TGGTAGCCCC GGCTTGGAGA GACACGTTAT GTATGGCTAT 
TCCCCCGCTG ATGACAAACG ACCGACACTG TCACGGCAGA GCAGTGCGTC CGGATACCAG 
GCACCGCCCA CCCCATCCTT CCCGGTCTCC CCGGCCTATT ACCATGGCAT GAGTAGCCCT 
TCCTCCTCCT CTCCTGACTC AACCTTATAC CGGCAAGGGA GCCCAATCTC ACAGCCGTCT 
CTGCCCGAGA AGAGGAGAAT GTCATCTGGG GACAGATCCA ACAGCCTCCC CAACTACTCC 
ACCCTCAATG GAAAAACCTC TTCGCCCATG TCCAGCGGGA TGTCCAGCCC CAGCGGCGGA 
AGCACAATGG CATTTACTCA CACTCTACCG GACTTCTCCA AGTTCTCCAT GCCAGACAGC 
AGCCCGGAGA CCCGAGCAAA TGTGAAGTTT GTGCAGGACA CGTCCAAGTA CTGGTACAAA 
CCAGACATTT CCAGGGATCA GGCCATCTCC CTGCTGAAGG ACAAAGACCC CGGGGCGTTC 
ATCATACGAG ACAGCCATTC GTTCCGAGGG GCCTACGGAC TTGCCATGAA AGTTGCCACC 
CCGCCCCCCA ACGCTGGCCA GCCCAGCAAG AAGGGAGATG TGACCAATGA GCTGGTTCGG 
CACTTTTTGA TTGAGACGAG TCCCAGGGGG GTGAAATTAA AGGGCTGCCA GAACGAACCC 
TACTTTGGCT GCCTGTCGGC TCTGGTGTAC CAGCACTCCA TTACTCCCCT TGCACTGCCC 
TGCAAACTGG TCATACCCTG CAGAGATCCC TCTGATGAGA CCAAGGAGAC GGCTGTTCCG 
GCCAATTCAG CCTCAGATTT ACTGAAACAG GGCGCAGCCT GCAACGTGTT ATTCATAAAC 
TCGATAGACA TGGAGTCGCT GACCGGCCCA CAAGCCATTG CGAAGACCAT CGCCGAGTCT 
CTCGCCGCCG ACCCCCCGCC GGCCGCCGCC ATCGTTCACT TCAAAGTCTC CGCCCAAGGA 
ATCACCTTAA CCGACAACCA GAGAAAATTA TTTTTCAGAC GACATTATCC GCTCAGCTCA 
GTCACCTTCT GCGACCTGGA CCCGCAAGAG AGAAAGTGGA TGAAGGCCGA TGGGACACAA 
GCCAAACTCT TTGGCTTTGT GGCCAGAAAG CAAGGAAGCA CCACAGACAA TATGTGCCAC 
CTGTTTGCCG AACTTGACCC AAACCAACCA GCCACTGCCA TCGTCAACTT TGTGTCCCGA 
GTCATCGGCC TGCAGAAGAG ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences.
Proceedings of the National Academy of Sciences of the United States of America99(26)16899-903(2002 Dec)
Strausberg RL,Feingold EA,Grouse LH,Derge JG,Klausner RD,Collins FS,Wagner L,Shenmen CM,Schuler GD,Altschul SF,Zeeberg B,Buetow KH,Schaefer CF,Bhat NK,Hopkins RF,Jordan H,Moore T,Max SI,Wang J,Hsieh F,Diatchenko L,Marusina K,Farmer AA,Rubin GM,Hong L,Stapleton M,Soares MB,Bonaldo MF,Casavant TL,Scheetz TE,Brownstein MJ,Usdin TB,Toshiyuki S,Carninci P,Prange C,Raha SS,Loquellano NA,Peters GJ,Abramson RD,Mullahy SJ,Bosak SA,McEwan PJ,McKernan KJ,Malek JA,Gunaratne PH,Richards S,Worley KC,Hale S,Garcia AM,Gay LJ,Hulyk SW,Villalon DK,Muzny DM,Sodergren EJ,Lu X,Gibbs RA,Fahey J,Helton E,Ketteman M,Madan A,Rodrigues S,Sanchez A,Whiting M,Madan A,Young AC,Shevchenko Y,Bouffard GG,Blakesley RW,Touchman JW,Green ED,Dickson MC,Rodriguez AC,Grimwood J,Schmutz J,Myers RM,Butterfield YS,Krzywinski MI,Skalska U,Smailus DE,Schnerch A,Schein JE,Jones SJ,Marra MA,

Genetic and genomic tools for Xenopus research: The NIH Xenopus initiative.
Developmental dynamics : an official publication of the American Association of Anatomists225(4)384-91(2002 Dec)
Klein SL,Strausberg RL,Wagner L,Pontius J,Clifton SW,Richardson P

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tns1 cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

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gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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