ak9 cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

Gene
Clones
gRNAs

Summary

Gene Symbol	ak9
Entrez Gene ID	100144994
Full Name	adenylate kinase 9
Synonyms	akd1,akd2
General protein information	Preferred Names adenylate kinase 9 Names LOW QUALITY PROTEIN: adenylate kinase 9 adenylate kinase 9 adenylate kinase domain containing 1
Gene Type	protein-coding
Organism	Xenopus tropicalis(tropical clawed frog)

mRNA and Protein(s)

mRNA	Protein	Name
XM_018094315.1	XP_017949804.1	LOW QUALITY PROTEIN: adenylate kinase 9
XM_031901891.1	XP_031757751.1	adenylate kinase 9 isoform X1
XM_031901892.1	XP_031757752.1	adenylate kinase 9 isoform X2

Pathways from BioSystems

Homologies

Danio rerio (zebrafish)	si:ch211-199m3.4	XP_685909.5
Mus musculus (house mouse)	Ak9	XP_006513002.1
Xenopus tropicalis (tropical clawed frog)	ak9	XP_002940598.2
Canis lupus familiaris (dog)	AK9	XP_005627761.1
Homo sapiens (human)	AK9	NP_001138600.2
Bos taurus (cattle)	AKD1	XP_005210887.1
Pan troglodytes (chimpanzee)	AKD1	XP_001153976.2
Macaca mulatta (Rhesus monkey)	LOC700039	XP_001088445.2
Rattus norvegicus (Norway rat)	RGD1560386	XP_006256619.1
Gallus gallus (chicken)	AKD1	XP_003641084.2

Gene Ontology

The following ak9 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ak9 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OXa10474	XM_018094315.1 Latest version!	Xenopus tropicalis adenylate kinase 9 (ak9), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OXa58177	XM_031901891.1 Latest version!	Xenopus tropicalis adenylate kinase 9 (ak9), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OXa58178	XM_031901892.1 Latest version!	Xenopus tropicalis adenylate kinase 9 (ak9), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

ak9 ( XM_018094315.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_017949804.1 Xenopus tropicalis adenylate kinase 9 (ak9), mRNA.

Price & Availability↑

CloneID	OXa10474
Clone ID Related Accession (Same CDS sequence)	XM_018094315.1
Accession Version	XM_018094315.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5466bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1473177600000
Organism	Xenopus tropicalis(tropical clawed frog)
Product	LOW QUALITY PROTEIN: adenylate kinase 9
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030681.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## frameshifts :: corrected 1 indel ##RefSeq-Attributes-END##

ATGGCTTCTC AAGAAAGGCC AACTTTTGCA GACATATTTG ATGAAGATGA AGCACAACAA 
TCCTTTCTCA TGTCCAAACC CACTTGCTTT CTCATTGTTG GAAAACCTGG AACTGGAAAA 
GCTACTTTAG GGAAGAAGAT TTCTCAGGCA TGGAACTCTA TTTTTATTGA AGCTAAAGAA 
CTGATTACTG ATCATATTAA AAGAGGGACG GATCAGGGCA ATAAGATGCA GGCGCTCCTT 
CATCAGGGCT TGAGTATTCC TGAGGAACTA GTAACACATT TAATTTTGGA CAAGATAAAT 
TCTCCAGAGG TTGCTCATCT TGGATATGTG CTATGCGGGT TTCCCTCTCT ATGTGAAGAA 
TATGTAAAGA TCCCCCAACA AATTGAACTG ATTAAAAATT TCAAGTTAAC CCCAGATATT 
ATAATTAATA TTAAGTGCCC AGACACAGAT CTAGGAAAAA GACTGTGTGG CCTAAAGCAA 
GATCCAAATA CAGGCATTAT TTATCAAAGA GAAGATTGGG ATCCTGCTCT GAAAGAAAAA 
CAGAAGAAGC AGCAAGTAGG AGAAGAGGGA GAAGAAGAGG AGGAGGAGGA GGAGGAGGAG 
GAAGAGTTGG AAGAAGAACA GGAAGAAGAA ATGTCGTTAA CAGAAAAGCT TGTTTATAGG 
CCTGAAGACT TTCCTGAAAA TGTTAAAGAA CGCATAAAGC TATTCAAAGA TATTCTTCTG 
CGCCCTGTGG AGGATCTAAT GATTGACCAT GATCCTCACT ATCTCATAGA GCTGGATGGA 
AATATGAAAC CACAGAAAAT TTTTGAAACA GTATTATCTC GTCTTGTATC TTTGGGGCTT 
CGACTTGGAT CAGTTGTGAT GAAGTTACAG AATTCCGAGG AAGAAGAAAC ACTGCAGGAA 
ACAGATACTG ATCAACTCTT GCGATTACTG GCATCTAGCT CTATGATTGC TCCAAGATAT 
AGGTGGCGCA GAAGCAGATG GGGTCAGACA TGTCCTGTTG CTTTAAAGGA AGGATTTATT 
AAAAGAGGAC TTGTGGAGTT TGCTGTTAGT TTCCTGGATA AAATGTATTT GATGTCTTCA 
GAAGAGGCAC TTAGAAAATT TATGCAGAAT CCTCGTCAGT ATCTCCTCCC TCCCATGCCA 
CTTCCACCTT GTAAAGTAGC AGTGGTTGGA CCGAAGTGCT CTGGAAAAAC CACCCTTTGC 
AATTTCATAG CCAATAAATA TGCAGGAAAG GTCCTTGATA TGTCTGTGCT TATAAAACCA 
TTTGCTGAAG AAGCTAAGAA AAATTTTATA GAAAAAGCCG GGGAGGAAGC AACTGAACTG 
GCTATTCAAA CTGTGACAAT GNGAGTACAA GAAGAGAGGA TTTTAAAAGA ACCAGACAAA 
CCAGAAGAAA CAGAGGGGAT AGAAGAGCTG GAGTATCAAA AGGAAATGCC AGAAACCTTA 
GCTGCAGAGG TATTGGAAGA AATTACTGCT GATCATCCAG AGGTCTTAGA AATAGTGGCT 
GAAGCTGTAA AAGCTGCAAG TGAGACTCCT GTGACCATTA CTCCAGATCT TTATATGGCT 
GCACTAGAAC AAGCAATAAA CAAGTTGCAT GAAGAAAATA AAGAAAGATT TACAAGTGAT 
CCACCAGCTG GGGGATGGGT ACTGGATAAT TTTCCTAATT CTCCAAACTG CTGGTTAGCA 
ATGGCGGACA GGGGATTTCT CCCAGACACT GTCATTTGTT TAAGAGATTC AGCAGATAAT 
GGAAAGTACT TGCTGAACAG ACTGTACCTT ATGAATGAAC AAGACATTAA TGATAATATC 
ATTCAAAGAC TTCAGAAAGA AAGAGCAAGG AAGCTACAAG AAGAGGAGGA GGCAAGGAAA 
GAACAGCTAG AAATAATGAG ATTGCAACAA GAGGAAAAAC AGAGTCAAGA AGAGCAGCAG 
GAAAGCTCAG AGGATGGCAT GGAAATGTCT ACAGAATTGA GTGAACAGCC TTCTCACATT 
GTTTCAGAGC AAGTTATATC TTCGGTGCCT TCTCCAAATG AAGAAGTTCA AAGTAACATG 
TCAGAACTAG AGGCTGGTGA CAAGACTCTT CTAAGTAACA CAGCTCCACT GCCGATTTCT 
TCCAAGGATG AGCACAACCT GGCAGAGCCC CAACCACAGC CAGAAATTAT TTTGCCAGAA 
GTCCCAGAGG GCGGATTCCC AGATGTGCCA GAAATGGAGC CACTGAAAGA GTTTATTACC 
AAGTTTAATG AAGGATGGAT TCTGATAGAA CCAATACTTA ATGATGCAGG AAACCCAATT 
CTCACATATT TGGAGATAAA AGAGAAATCA CCTGAGACAA TGCTACAAGA AACCATCAGT 
TCTATGGAAA AACCATTTAA GTATTATGGC TGGGAAATGA CATCAGAAGA CATAGATGAA 
GAAGCTGAAG ACATGCAAGC CGAGCTAGAT TCTGAGGAAC AAGAACAGGA GGAGAATGAA 
AATGAGAGTG GAGGAGAAGA AGAAGAAGAA GATGAAGATC GCATTCGTGA AAGGAAGAGA 
AACTATGGGG ATTCCAAACA CTTTTGTCCA GTGGCCCTAA AAGATAATTT TGTGCTGTAT 
CCTGGAGAAT CTGAATATGC AGCAGTATAC AGGGAGAAGC TTTATTACTG TTCAACTCCT 
GAAGCAAGGG ACAAGTTCCT AGACAATCCT GAGAATTATA TAATTAACAA AGAACCTTTA 
AAGGCTCCAC CATTGAGAGT GCTTTTAATT GGAAACAGTG GTGCTGGCAA AACAGTTTGT 
GCAAGGTGGT TGGCAGAAAA ATTAGGCATT TTCCACATAC AGTTTAGAGA GTACCTGCAA 
GAGATAATGC TTTCCAAACT TCAAAGGAAA ATTGGACCAC AGTATGCTGA GGAAGTAAAA 
GAGTATCCAA ATGAGGAAAC TCCTCTAATG GAACTAGAAA ATAGTGACTT AGCTATTGCC 
GAAGAAAACA CTGCAGAGGA AGAGGTGGTT TTAACAGATG AAGAAGAAGC AGTCAAATCA 
TATTTAGAAG ATGCATCCCC ATTGCCAGCT GAACTGCTGG ATCAGATTGT AACAGACTGG 
TGGTCAGAAG AACCTTTTCG CTCAACTGGG TTTATATTAG ATGGCTTTCC TAATACAGCA 
GAAGAGACAC AGTACTTAGG GGAGAAGGGC TTTTTCCCAG ACATTGCTGT CTTTCTTGAT 
GTAGAAGAAA GTGCCATTTG CGACCGCCTT CTTCCTCCAC GTTTGGCAAA ATGGAAAGAA 
CGTCAAATGA GAAAAGAGGA GCGCAAACAA AAATTAAAAG CACTCAAGAA ACAAATAAGG 
GACGATCAGA TTGCAAAGAG AAGGGCTGAA TTAATGGCTG AACAAAATAA AATGAAAGAA 
GAAAAAGAGA ATCAAAAAAA CCCAGATGAA CAGGAGGAGG AGGAGGAGGA AGAAGATGAT 
GAAGGGGATG ATATTGAACT CATTCTTTCA GAAGAGTTTC CAGATGAGGA AGAAGATGAT 
GAGGAAGAAG AGGAGTCAGA ATATGATGCA GTTGAAAGAA TGAAAAGTGA AATAGGGGAG 
AAGTGTGAAG CTGAAGCAGA GTCTCTAGAA ACAGTGAAGG ATGAGCTTCA GAGACTTAAG 
ATCCCTGTTG TGACAATAAA TGGAGGCCGG AAACGACATA TTGTGCACTA CCAGTTATAT 
GAGAAGCTAA GAAATCTTGT AGAAAACCGT GAAAGCCTCT TTGAAAAAAG CTACCCCATT 
AGCCTTGCTG TTGCTAATAA GATGCTCCAA ATGTCTTACA AACAGCCTAG TGTCTTTGGA 
CGGTGGGACC CAGTCAAGCT AAGCCAAGGA GAGGTAATAA AACCATTTTT CACCCAAGAA 
AATCCCGGCT TTCCATTACT ATACCGCCAA TATATTTACT TCTTCTCGAC AAAGGAAAAC 
AGGAACTTAT TTATGAAAAA TCCTATCAAA TACATCCAGC AGGCAAAACC CAAGCCATCA 
GTGCCAATAA GAATAGCTGT TGTTGGGCCT CCTAAATCTG GCAAGACTTC AGTTGCTAAG 
ATGTTTACGA GTTTATATGG GCTGAAAAGA CTTTCAATTG GAGATGCCAT TCGCTCTGTT 
TTGGAAAATC AGCCAAACAC TGAATTGGCA ACAGAATTAA ACTCTATACT TCGGAAAGGG 
TTGGTTTTGC CTGATGATCT TTCTGTTAAG TGTCTAGAAG TAGCTTTGAT GGATTTAACT 
TCTAATACAA ATGGAGTTGT TTTTGATGGT TTTCCTGTTA CCAAACATCA AGTAGATCTT 
CTGGAAGCCT GCAGTATAAT TCCTATAAAA ATATTTGAGC TGCAAGCCCC TATAAAGGAA 
GTGTTAAAAA GAGGACTGGA AGACAAGAAG AACTCTAACA GGCCTTACCC TGTGCATGAT 
AGTGCTCAAA TTCTTGTTGT ACGCAACTCC TGTTATAAAC AAGAAGTCAT TCTGACCAAA 
GAACACTACT CAAAGCTGCA TCAGAACTGG TGTGAAGTTG ATTCTTTAAG GAGTAAATGG 
TGGGTTTCCA ACAAAATTAC TGGAGAAGTT CAGAAAAGTA TCAATCAAAT ACAGTCCTAC 
TTGGAGAGAA TAAAAGAAGG TATGGCAGCT GGCATTGCTG ACTTCTGCAT CACTCCTCAG 
GAGCTAGTTT CTCGGCTGGG TGAGTTTGGA CAATACTGCC CTGTGTGTCT AGCACAAAGG 
GGAGAGCTTG TTGACTGTTC TGGAAATCCA TCCCTGCAGT TTGCTGCAGA ATTCCGGGGA 
CACTATTACA AGATGGCTTC ACAGAAGGAG CTGGATGCTT TCTTGAAAAG TCCTGAATTA 
TATGTGCCAC CTCTTGCACC GCGCCCAATC CCTCCTCCTG AGCTGTTACC AAAAACGCTA 
ACTGTGGCTG ATGTGAAGGC AAAATTTCCT AAAAATGCAG AAATGAAGGG GTATTGCCCA 
GTTACCTATG TTGATGGTAA TAAAAGATAC GAAGCACTGG TCCCTGGTCA TATTGAGAAT 
GCAGTAGAAT ACAGAGATAA GATTTATATT TTTGAGACCA AGGAAAAGCT ACAGAAGTTC 
ATGAGACTTC CAGAGAAATA CTACAATCAG AAGCTTCCAT GCAAACTTCC ACCAAAAATA 
GAACCTGTGC TTTTGACTTC ACTTCCATTA ACTGGATACC TTGAGCAGGG AGCTGCAACT 
GCTTTAATAA AAGCAATGAA TGATGTAGGC TGCCTAAAGC CCAAGTATCC ATATCTAAAC 
ATCAAACAAT CTGCTCTGCT CTATATTGCT TTTCATTTAA AAGCTTTTAA TCCTCGGAAT 
TCTGATTACA TACGAAATAA ATACAAGAAA AAGCTGGAAC AATTTGTGGA GCGATGTGAA 
CTTATAACAT ACCTTGGAGA CACAATGACT CGCAAGTACA AAGAACCCCA AAGAAGGCCC 
ATTGATTTTG ACTTTAAGAT GCAGTCTTTC TTGTCATTGA AAGGCATGGA CCCAACCTGC 
ATTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017949804.1
CDS	28..5493
Translation	MASQERPTFADIFDEDEAQQSFLMSKPTCFLIVGKPGTGKATLGKKISQAWNSIFIEAKELITDHIKRGTDQGNKMQALLHQGLSIPEELVTHLILDKINSPEVAHLGYVLCGFPSLCEEYVKIPQQIELIKNFKLTPDIIINIKCPDTDLGKRLCGLKQDPNTGIIYQREDWDPALKEKQKKQQVGEEGEEEEEEEEEEEELEEEQEEEMSLTEKLVYRPEDFPENVKERIKLFKDILLRPVEDLMIDHDPHYLIELDGNMKPQKIFETVLSRLVSLGLRLGSVVMKLQNSEEEETLQETDTDQLLRLLASSSMIAPRYRWRRSRWGQTCPVALKEGFIKRGLVEFAVSFLDKMYLMSSEEALRKFMQNPRQYLLPPMPLPPCKVAVVGPKCSGKTTLCNFIANKYAGKVLDMSVLIKPFAEEAKKNFIEKAGEEATELAIQTVTMnullVQEERILKEPDKPEETEGIEELEYQKEMPETLAAEVLEEITADHPEVLEIVAEAVKAASETPVTITPDLYMAALEQAINKLHEENKERFTSDPPAGGWVLDNFPNSPNCWLAMADRGFLPDTVICLRDSADNGKYLLNRLYLMNEQDINDNIIQRLQKERARKLQEEEEARKEQLEIMRLQQEEKQSQEEQQESSEDGMEMSTELSEQPSHIVSEQVISSVPSPNEEVQSNMSELEAGDKTLLSNTAPLPISSKDEHNLAEPQPQPEIILPEVPEGGFPDVPEMEPLKEFITKFNEGWILIEPILNDAGNPILTYLEIKEKSPETMLQETISSMEKPFKYYGWEMTSEDIDEEAEDMQAELDSEEQEQEENENESGGEEEEEDEDRIRERKRNYGDSKHFCPVALKDNFVLYPGESEYAAVYREKLYYCSTPEARDKFLDNPENYIINKEPLKAPPLRVLLIGNSGAGKTVCARWLAEKLGIFHIQFREYLQEIMLSKLQRKIGPQYAEEVKEYPNEETPLMELENSDLAIAEENTAEEEVVLTDEEEAVKSYLEDASPLPAELLDQIVTDWWSEEPFRSTGFILDGFPNTAEETQYLGEKGFFPDIAVFLDVEESAICDRLLPPRLAKWKERQMRKEERKQKLKALKKQIRDDQIAKRRAELMAEQNKMKEEKENQKNPDEQEEEEEEEDDEGDDIELILSEEFPDEEEDDEEEEESEYDAVERMKSEIGEKCEAEAESLETVKDELQRLKIPVVTINGGRKRHIVHYQLYEKLRNLVENRESLFEKSYPISLAVANKMLQMSYKQPSVFGRWDPVKLSQGEVIKPFFTQENPGFPLLYRQYIYFFSTKENRNLFMKNPIKYIQQAKPKPSVPIRIAVVGPPKSGKTSVAKMFTSLYGLKRLSIGDAIRSVLENQPNTELATELNSILRKGLVLPDDLSVKCLEVALMDLTSNTNGVVFDGFPVTKHQVDLLEACSIIPIKIFELQAPIKEVLKRGLEDKKNSNRPYPVHDSAQILVVRNSCYKQEVILTKEHYSKLHQNWCEVDSLRSKWWVSNKITGEVQKSINQIQSYLERIKEGMAAGIADFCITPQELVSRLGEFGQYCPVCLAQRGELVDCSGNPSLQFAAEFRGHYYKMASQKELDAFLKSPELYVPPLAPRPIPPPELLPKTLTVADVKAKFPKNAEMKGYCPVTYVDGNKRYEALVPGHIENAVEYRDKIYIFETKEKLQKFMRLPEKYYNQKLPCKLPPKIEPVLLTSLPLTGYLEQGAATALIKAMNDVGCLKPKYPYLNIKQSALLYIAFHLKAFNPRNSDYIRNKYKKKLEQFVERCELITYLGDTMTRKYKEPQRRPIDFDFKMQSFLSLKGMDPTCI

Target ORF information:

RefSeq Version	XM_018094315.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis adenylate kinase 9 (ak9), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_018094315.1

ORF Insert Sequence:

ATGGCTTCTC AAGAAAGGCC AACTTTTGCA GACATATTTG ATGAAGATGA AGCACAACAA 
TCCTTTCTCA TGTCCAAACC CACTTGCTTT CTCATTGTTG GAAAACCTGG AACTGGAAAA 
GCTACTTTAG GGAAGAAGAT TTCTCAGGCA TGGAACTCTA TTTTTATTGA AGCTAAAGAA 
CTGATTACTG ATCATATTAA AAGAGGGACG GATCAGGGCA ATAAGATGCA GGCGCTCCTT 
CATCAGGGCT TGAGTATTCC TGAGGAACTA GTAACACATT TAATTTTGGA CAAGATAAAT 
TCTCCAGAGG TTGCTCATCT TGGATATGTG CTATGCGGGT TTCCCTCTCT ATGTGAAGAA 
TATGTAAAGA TCCCCCAACA AATTGAACTG ATTAAAAATT TCAAGTTAAC CCCAGATATT 
ATAATTAATA TTAAGTGCCC AGACACAGAT CTAGGAAAAA GACTGTGTGG CCTAAAGCAA 
GATCCAAATA CAGGCATTAT TTATCAAAGA GAAGATTGGG ATCCTGCTCT GAAAGAAAAA 
CAGAAGAAGC AGCAAGTAGG AGAAGAGGGA GAAGAAGAGG AGGAGGAGGA GGAGGAGGAG 
GAAGAGTTGG AAGAAGAACA GGAAGAAGAA ATGTCGTTAA CAGAAAAGCT TGTTTATAGG 
CCTGAAGACT TTCCTGAAAA TGTTAAAGAA CGCATAAAGC TATTCAAAGA TATTCTTCTG 
CGCCCTGTGG AGGATCTAAT GATTGACCAT GATCCTCACT ATCTCATAGA GCTGGATGGA 
AATATGAAAC CACAGAAAAT TTTTGAAACA GTATTATCTC GTCTTGTATC TTTGGGGCTT 
CGACTTGGAT CAGTTGTGAT GAAGTTACAG AATTCCGAGG AAGAAGAAAC ACTGCAGGAA 
ACAGATACTG ATCAACTCTT GCGATTACTG GCATCTAGCT CTATGATTGC TCCAAGATAT 
AGGTGGCGCA GAAGCAGATG GGGTCAGACA TGTCCTGTTG CTTTAAAGGA AGGATTTATT 
AAAAGAGGAC TTGTGGAGTT TGCTGTTAGT TTCCTGGATA AAATGTATTT GATGTCTTCA 
GAAGAGGCAC TTAGAAAATT TATGCAGAAT CCTCGTCAGT ATCTCCTCCC TCCCATGCCA 
CTTCCACCTT GTAAAGTAGC AGTGGTTGGA CCGAAGTGCT CTGGAAAAAC CACCCTTTGC 
AATTTCATAG CCAATAAATA TGCAGGAAAG GTCCTTGATA TGTCTGTGCT TATAAAACCA 
TTTGCTGAAG AAGCTAAGAA AAATTTTATA GAAAAAGCCG GGGAGGAAGC AACTGAACTG 
GCTATTCAAA CTGTGACAAT GNGAGTACAA GAAGAGAGGA TTTTAAAAGA ACCAGACAAA 
CCAGAAGAAA CAGAGGGGAT AGAAGAGCTG GAGTATCAAA AGGAAATGCC AGAAACCTTA 
GCTGCAGAGG TATTGGAAGA AATTACTGCT GATCATCCAG AGGTCTTAGA AATAGTGGCT 
GAAGCTGTAA AAGCTGCAAG TGAGACTCCT GTGACCATTA CTCCAGATCT TTATATGGCT 
GCACTAGAAC AAGCAATAAA CAAGTTGCAT GAAGAAAATA AAGAAAGATT TACAAGTGAT 
CCACCAGCTG GGGGATGGGT ACTGGATAAT TTTCCTAATT CTCCAAACTG CTGGTTAGCA 
ATGGCGGACA GGGGATTTCT CCCAGACACT GTCATTTGTT TAAGAGATTC AGCAGATAAT 
GGAAAGTACT TGCTGAACAG ACTGTACCTT ATGAATGAAC AAGACATTAA TGATAATATC 
ATTCAAAGAC TTCAGAAAGA AAGAGCAAGG AAGCTACAAG AAGAGGAGGA GGCAAGGAAA 
GAACAGCTAG AAATAATGAG ATTGCAACAA GAGGAAAAAC AGAGTCAAGA AGAGCAGCAG 
GAAAGCTCAG AGGATGGCAT GGAAATGTCT ACAGAATTGA GTGAACAGCC TTCTCACATT 
GTTTCAGAGC AAGTTATATC TTCGGTGCCT TCTCCAAATG AAGAAGTTCA AAGTAACATG 
TCAGAACTAG AGGCTGGTGA CAAGACTCTT CTAAGTAACA CAGCTCCACT GCCGATTTCT 
TCCAAGGATG AGCACAACCT GGCAGAGCCC CAACCACAGC CAGAAATTAT TTTGCCAGAA 
GTCCCAGAGG GCGGATTCCC AGATGTGCCA GAAATGGAGC CACTGAAAGA GTTTATTACC 
AAGTTTAATG AAGGATGGAT TCTGATAGAA CCAATACTTA ATGATGCAGG AAACCCAATT 
CTCACATATT TGGAGATAAA AGAGAAATCA CCTGAGACAA TGCTACAAGA AACCATCAGT 
TCTATGGAAA AACCATTTAA GTATTATGGC TGGGAAATGA CATCAGAAGA CATAGATGAA 
GAAGCTGAAG ACATGCAAGC CGAGCTAGAT TCTGAGGAAC AAGAACAGGA GGAGAATGAA 
AATGAGAGTG GAGGAGAAGA AGAAGAAGAA GATGAAGATC GCATTCGTGA AAGGAAGAGA 
AACTATGGGG ATTCCAAACA CTTTTGTCCA GTGGCCCTAA AAGATAATTT TGTGCTGTAT 
CCTGGAGAAT CTGAATATGC AGCAGTATAC AGGGAGAAGC TTTATTACTG TTCAACTCCT 
GAAGCAAGGG ACAAGTTCCT AGACAATCCT GAGAATTATA TAATTAACAA AGAACCTTTA 
AAGGCTCCAC CATTGAGAGT GCTTTTAATT GGAAACAGTG GTGCTGGCAA AACAGTTTGT 
GCAAGGTGGT TGGCAGAAAA ATTAGGCATT TTCCACATAC AGTTTAGAGA GTACCTGCAA 
GAGATAATGC TTTCCAAACT TCAAAGGAAA ATTGGACCAC AGTATGCTGA GGAAGTAAAA 
GAGTATCCAA ATGAGGAAAC TCCTCTAATG GAACTAGAAA ATAGTGACTT AGCTATTGCC 
GAAGAAAACA CTGCAGAGGA AGAGGTGGTT TTAACAGATG AAGAAGAAGC AGTCAAATCA 
TATTTAGAAG ATGCATCCCC ATTGCCAGCT GAACTGCTGG ATCAGATTGT AACAGACTGG 
TGGTCAGAAG AACCTTTTCG CTCAACTGGG TTTATATTAG ATGGCTTTCC TAATACAGCA 
GAAGAGACAC AGTACTTAGG GGAGAAGGGC TTTTTCCCAG ACATTGCTGT CTTTCTTGAT 
GTAGAAGAAA GTGCCATTTG CGACCGCCTT CTTCCTCCAC GTTTGGCAAA ATGGAAAGAA 
CGTCAAATGA GAAAAGAGGA GCGCAAACAA AAATTAAAAG CACTCAAGAA ACAAATAAGG 
GACGATCAGA TTGCAAAGAG AAGGGCTGAA TTAATGGCTG AACAAAATAA AATGAAAGAA 
GAAAAAGAGA ATCAAAAAAA CCCAGATGAA CAGGAGGAGG AGGAGGAGGA AGAAGATGAT 
GAAGGGGATG ATATTGAACT CATTCTTTCA GAAGAGTTTC CAGATGAGGA AGAAGATGAT 
GAGGAAGAAG AGGAGTCAGA ATATGATGCA GTTGAAAGAA TGAAAAGTGA AATAGGGGAG 
AAGTGTGAAG CTGAAGCAGA GTCTCTAGAA ACAGTGAAGG ATGAGCTTCA GAGACTTAAG 
ATCCCTGTTG TGACAATAAA TGGAGGCCGG AAACGACATA TTGTGCACTA CCAGTTATAT 
GAGAAGCTAA GAAATCTTGT AGAAAACCGT GAAAGCCTCT TTGAAAAAAG CTACCCCATT 
AGCCTTGCTG TTGCTAATAA GATGCTCCAA ATGTCTTACA AACAGCCTAG TGTCTTTGGA 
CGGTGGGACC CAGTCAAGCT AAGCCAAGGA GAGGTAATAA AACCATTTTT CACCCAAGAA 
AATCCCGGCT TTCCATTACT ATACCGCCAA TATATTTACT TCTTCTCGAC AAAGGAAAAC 
AGGAACTTAT TTATGAAAAA TCCTATCAAA TACATCCAGC AGGCAAAACC CAAGCCATCA 
GTGCCAATAA GAATAGCTGT TGTTGGGCCT CCTAAATCTG GCAAGACTTC AGTTGCTAAG 
ATGTTTACGA GTTTATATGG GCTGAAAAGA CTTTCAATTG GAGATGCCAT TCGCTCTGTT 
TTGGAAAATC AGCCAAACAC TGAATTGGCA ACAGAATTAA ACTCTATACT TCGGAAAGGG 
TTGGTTTTGC CTGATGATCT TTCTGTTAAG TGTCTAGAAG TAGCTTTGAT GGATTTAACT 
TCTAATACAA ATGGAGTTGT TTTTGATGGT TTTCCTGTTA CCAAACATCA AGTAGATCTT 
CTGGAAGCCT GCAGTATAAT TCCTATAAAA ATATTTGAGC TGCAAGCCCC TATAAAGGAA 
GTGTTAAAAA GAGGACTGGA AGACAAGAAG AACTCTAACA GGCCTTACCC TGTGCATGAT 
AGTGCTCAAA TTCTTGTTGT ACGCAACTCC TGTTATAAAC AAGAAGTCAT TCTGACCAAA 
GAACACTACT CAAAGCTGCA TCAGAACTGG TGTGAAGTTG ATTCTTTAAG GAGTAAATGG 
TGGGTTTCCA ACAAAATTAC TGGAGAAGTT CAGAAAAGTA TCAATCAAAT ACAGTCCTAC 
TTGGAGAGAA TAAAAGAAGG TATGGCAGCT GGCATTGCTG ACTTCTGCAT CACTCCTCAG 
GAGCTAGTTT CTCGGCTGGG TGAGTTTGGA CAATACTGCC CTGTGTGTCT AGCACAAAGG 
GGAGAGCTTG TTGACTGTTC TGGAAATCCA TCCCTGCAGT TTGCTGCAGA ATTCCGGGGA 
CACTATTACA AGATGGCTTC ACAGAAGGAG CTGGATGCTT TCTTGAAAAG TCCTGAATTA 
TATGTGCCAC CTCTTGCACC GCGCCCAATC CCTCCTCCTG AGCTGTTACC AAAAACGCTA 
ACTGTGGCTG ATGTGAAGGC AAAATTTCCT AAAAATGCAG AAATGAAGGG GTATTGCCCA 
GTTACCTATG TTGATGGTAA TAAAAGATAC GAAGCACTGG TCCCTGGTCA TATTGAGAAT 
GCAGTAGAAT ACAGAGATAA GATTTATATT TTTGAGACCA AGGAAAAGCT ACAGAAGTTC 
ATGAGACTTC CAGAGAAATA CTACAATCAG AAGCTTCCAT GCAAACTTCC ACCAAAAATA 
GAACCTGTGC TTTTGACTTC ACTTCCATTA ACTGGATACC TTGAGCAGGG AGCTGCAACT 
GCTTTAATAA AAGCAATGAA TGATGTAGGC TGCCTAAAGC CCAAGTATCC ATATCTAAAC 
ATCAAACAAT CTGCTCTGCT CTATATTGCT TTTCATTTAA AAGCTTTTAA TCCTCGGAAT 
TCTGATTACA TACGAAATAA ATACAAGAAA AAGCTGGAAC AATTTGTGGA GCGATGTGAA 
CTTATAACAT ACCTTGGAGA CACAATGACT CGCAAGTACA AAGAACCCCA AAGAAGGCCC 
ATTGATTTTG ACTTTAAGAT GCAGTCTTTC TTGTCATTGA AAGGCATGGA CCCAACCTGC 
ATTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ak9 ( XM_031901891.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_031757751.1 Xenopus tropicalis adenylate kinase 9 (ak9), transcript variant X1, mRNA.

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CloneID	OXa58177
Clone ID Related Accession (Same CDS sequence)	XM_031901891.1
Accession Version	XM_031901891.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5463bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1576512000000
Organism	Xenopus tropicalis(tropical clawed frog)
Product	adenylate kinase 9 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030681.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTTCTC AAGAAAGGCC AACTTTTGCA GACATATTTG ATGAAGATGA AGCACAACAA 
TCCTTTCTCA TGTCCAAACC CACTTGCTTT CTCATTGTTG GAAAACCTGG AACTGGAAAA 
GCTACTTTAG GGAAGAAGAT TTCTCAGGCA TGGAACTCTA TTTTTATTGA AGCTAAAGAA 
CTGATTACTG ATCATATTAA AAGAGGGACG GATCAGGGCA ATAAGATGCA GGCGCTCCTT 
CATCAGGGCT TGAGTATTCC TGAGGAACTA GTAACACATT TAATTTTGGA CAAGATAAAT 
TCTCCAGAGG TTGCTCATCT TGGATATGTG CTATGCGGGT TTCCCTCTCT ATGTGAAGAA 
TATGTAAAGA TCCCCCAACA AATTGAACTG ATTAAAAATT TCAAGTTAAC CCCAGATATT 
ATAATTAATA TTAAGTGCCC AGACACAGAT CTAGGAAAAA GACTGTGTGG CCTAAAGCAA 
GATCCAAATA CAGGCATTAT TTATCAAAGA GAAGATTGGG ATCCTGCTCT GAAAGAAAAA 
CAGAAGAAGC AGCAAGTAGG AGAAGAGGGA GAAGAAGAGG AGGAGGAGGA GGAGGAGGAA 
GAGTTGGAAG AAGAACAGGA AGAAGAAATG TCGTTAACAG AAAAGCTTGT TTATAGGCCT 
GAAGACTTTC CTGAAAATGT TAAAGAACGC ATAAAGCTAT TCAAAGATAT TCTTCTGCGC 
CCTGTGGAGG ATCTAATGAT TGACCATGAT CCTCACTATC TCATAGAGCT GGATGGAAAT 
ATGAAACCAC AGAAAATTTT TGAAACAGTA TTATCTCGTC TTGTATCTTT GGGGCTTCGA 
CTTGGATCAG TTGTGATGAA GTTACAGAAT TCCGAGGAAG AAGAAACACT GCAGGAAACA 
GATACTGATC AACTCTTGCG ATTACTGGCA TCTAGCTCTA TGATTGCTCC AAGATATAGG 
TGGCGCAGAA GCAGATGGGG TCAGACATGT CCTGTTGCTT TAAAGGAAGG ATTTATTAAA 
AGAGGACTTG TGGAGTTTGC TGTTAGTTTC CTGGATAAAA TGTATTTGAT GTCTTCAGAA 
GAGGCACTTA GAAAATTTAT GCAGAATCCT CGTCAGTATC TTCTCCCTCC CATGCCACTT 
TCACCTTGTA AAGTAGCAGT GGTTGGACCG AAGTGCTCTG GAAAAACCAC CCTTTGCAAT 
TTCATAGCCA ATAAATATGC AGGAAAGGTC CTTGATATGT CTGTGCTTAT AAAACCATTT 
GCTGAAGAAG CTAAGAAAAA TTTTATAGAA AAAGCGGTGG AGGAAGCAAC TGAACTGGCT 
ATTAAAACTG TGAGAATGAA GTTACAAGAA GAGAGGATTT TAAAAGAACA AGACAAACCA 
GAAGAAACAG AGGGGATAGA AGAGCTGGAG TATCAAAAGG AAATGCCAGA AACCTTAGCT 
GCAGAGGTAT TGGAAGAAAT TACTGCTGAT CATCCAGAGG TCTTAGAAAT AGTGGCTGAA 
GCTGTAAAAG CTGCAAGTGA GACTCCTGTG ACCATTACTC CAGATCTTTA TATGGCTGCA 
CTAGAACAAG CAATAAACAA GTTGCATGAA GAAAATAAAG AAAGATTTAC AAGTGATCCA 
CCAGCTGGGG GATGGGTACT GGATAATTTT CCTAATTCTC CAAACTGCTG GTTAGCAATG 
GCGGACAGGG GATTTCTCCC AGACACTGTC ATTTGTTTAA GAGATTCAGC AGATAATGGA 
AAGTACTTGC TGAACAGACT GTACCTTATG AATGAACAAG ACATTAATGA TAATATCATT 
CAAAGACTTC AGAAAGAAAG AGCAAGGAAG CTACAAGAAG AGGAGGAGGC AAGGAAAGAA 
CAGCTAGAAA TAATGAGATT GCAACAAGAG GAAAAACAGA GGCAAGAAGA GCAGCAGGAA 
AGCTCAGAGG ATGGCATGGA AATGTCTACA GAATTGAGTG AACAGCCTTC TCACATTGTT 
TCAGAGCAAG TTATATCTTC GGTGCCTTCT CCAAATGAAG AAGTTCAAAG TAACATGTCA 
GAACTAGAGG CTGGTGACAA GACTCTTCTA AGTAACACAG CTCCACTGCC GATTTCTTCC 
AAGGATGAGC ACAACCTGGC AGAGCCCCAA CCACAGCCAG AAATTATTTT GCCAGAAGTC 
CCAGAGGGCG GATTCCCAGA TGTGCCAGAA ATGGAGCCAC TGAAAGAGTT TATTACCAAG 
TTTAATGAAG GATGGATTCT GATAGAACCA ATACTTAATG ATGCAGGAAA CCCAATTCTC 
ACATATTTGG AGATAAAAGA GAAATCACCT GAGACAATGC TACAAGAAAC CATCAGTTCT 
ATGGATAAAC CATTTAAGTA TTATGGCTGG GAAATGACAT CAGAAGACAT AGATGAAGAA 
GCTGAAGACA TGCAAGCCGA GCTAGATTCT GAGGAACAAG AACAGGAGGA GAATGAAAAT 
GAGAGTGGAG GAGAAGAAGA AGAAGAAGAT GAAGATCGCA TTCGTGAAAG GAAGAGAAAC 
TATGGGGATT CCAAACACTT TTGTCCAGTG GCCCTAAAAG ATAATTTTGT GCTGTATCCT 
GGAGAATCTG AATATGCAGC AGTATACAGG GAGAAGCTTT ATTACTGTTC AACTCCTGAA 
GCAAGGGACA AGTTCCTAGA CAATCCTGAG AATTATATAA TTAACAAAGA ACCTTTAAAG 
GCTCCACCAT TGAGAGTGCT TTTAATTGGA AACAGTGGTG CTGGCAAAAC AGTTTGTGCA 
AGGTGGTTGG CAGAAAAATT AGGCATTTTC CACATACAGT TTAGAGAGTA CCTGCAAGAG 
ATAATGCTTT CCAAACTTCA AAGGAAAATT GGACCACAGT ATGCTGAGGA AGTAAAAGAG 
TATCCAAATG AGGAAACTCC TCTAATGGAA CTAGAAAATA GTGACTTAGC TATTGCCGAA 
GAAAACACTG CAGAGGAAGA GGTGGTTTTA ACAGATGAAG AAGAAGCAGT CAAATCATAT 
TTAGAAGATG CATCCCCATT GCCAGCTGAA CTGCTGGATC AGATTGTAAC AGACTGGTGG 
TCAGAAGAAC CTTTTCGCTC AACTGGGTTT ATATTAGATG GCTTTCCTAA TACAGCAGAA 
GAGACACAGT ACTTAGGGGA GAAGGGCTTT TTCCCAGACA TTGCTGTCTT TCTTGATGTA 
GAAGAAAGTG CCATTTGCGA CCGCCTTCTT CCTCCACGTT TGGCAAAATG GAAAGAACGT 
CAAATGAGAA AAGAGGAGCG CAAACAAAAA TTAAAAGCAC TCAAGAAACA AATAAGGGAC 
GATCAGATTG CAAAGAGAAG GGCTGAATTA ATGGCTGAAC AAAATAAAAT GAAAGAAGAA 
AAAGAGAATC AAAAAAACCC AGATGAACAG GAGGAGGAGG AGGAGGAAGA AGGTGATGAA 
GGGGATGATA TTGAACTCAT TCTTTCAGAA GAGTTTCCAG ATGAGGAAGA AGATGATGAG 
GAAGAAGAGG AGTCAGAATA TGATGCAGTT GAAAGAATGA AAAGTGAAAT AGGGGAGAAG 
TGTGAAGCTG AAGCAGAGTC TCTAGAAACA GTGAAGGATG AGCTTCAGAG ACTTAAGATC 
CCTGTTGTGA CAATAAATGG AGGCCGGAAA CGACATATTG TGCACTACCA GTTATATGAG 
AAGCTAAGAA ATCTTGTAGA AAACCGTGAA AGCCTCTTTG AAAAAAGCTA CCCCATTAGC 
CTTGCTGTTG CTAATAAGAT GCTCCAAATG TCTTACAAAC AGCCTAGTGT CTTTGGACGG 
TGGGACCCAG TCAAGCTAAG CCAAGGAGAG GTAATAAAAC CATTTTTCAC CCAAGAAAAT 
CCCGGCTTTC CATTACTATA CCGCCAATAT ATTTACTTCT TCTCGACAAA GGAAAACAGG 
AACTTATTTA TGAAAAATCC TATCAAATAC ATCCAGCAGG CAAAACCCAA GCCATCAGTG 
CCAATAAGAA TAGCTGTTGT TGGGCCTCCT AAATCTGGCA AGACTTCAGT TGCTAAGATG 
TTTACGAGTT TATATGGGCT GAAAAGACTT TCAATTGGAG ATGCCATTCG CTCTGTTTTG 
GAAAATCAGC CAAACACTGA ATTGGCAACA GAATTAAACT CTATACTTCG GAAAGGGTTG 
GTTTTGCCTG ATGATCTTTC TGTTAAGTGT CTAGAAGTAG CTTTGATGGA TTTAACTTCT 
AATACAAATG GAGTTGTTTT TGATGGTTTT CCTGTTACCA AACATCAAGT AGATCTTCTG 
GAAGCCTGCA GTATAATTCC TATAAAAATA TTTGAGCTGC AAGCCCCTAT AAAGGAAGTG 
TTAAAAAGAG GACTGGAAGA CAAGAAGAAC TCTAACAGGC CTTACCCTGT GCATGATAGT 
GCTCAAATTC TTGTTGTACG CAACTCCTGT TATAAACAAG AAGTCATTCT GACCAAAGAA 
CACTACTCAA AGCTGCATCA GAACTGGTGT GAAGTTGATT CTTTAAGGAG TAAATGGTGG 
GTTTCCAACA AAATTACTGG AGAAGTTCAG AAAAGTATCA ATCAAATACA GTCCTACTTG 
GAGAGAATAA AAGAAGGTAT GGCAGCTGGC ATTGCTGACT TCTGCATCAC TCCTCAGGAG 
CTAGTTTCTC GGCTGGGTGA GTTTGGACAA TACTGCCCTG TGTGTCTAGC ACAAAGGGGA 
GAGCTTGTTG ACTGTTCTGG AAATCCATCC CTGCAGTTTG CTGCAGAATT CCGGGGACAC 
TATTACAAGA TGGCTTCACA GAAGGAGCTG GATGCTTTCT TGAAAAGTCC TGAATTATAT 
GTGCCACCTC TTGCACCGCG CCCAATCCCT CCTCCTGAGC TGTTACCAAA AACGCTAACT 
GTGGCTGATG TGAAGGCAAA ATTTCCTAAA AATGCAGAAA TGAAGGGGTA TTGCCCAGTT 
ACCTATGTTG ATGGTAATAA AAGATACGAA GCACTGGTCC CTGGTCATAT TGAGAATGCA 
GTAGAATACA GAGATAAGAT TTATATTTTT GAGACCAAGG AAAAGCTACA GAAGTTCATG 
AGACTTCCAG AGAAATACTA CAATCAGAAG CTTCCATGCA AACTTCCACC AAAAATAGAA 
CCTGTGCTTT TGACTTCACT TCCATTAACT GGATACCTTG AGCAGGGAGC TGCAACTGCT 
TTAATAAAAG CAATGAATGA TGTAGGCTGC CTAAAGCCCA AGTATCCATA TCTAAACATC 
AAACAATCTG CTCTGCTCTA TATTGCTTTT CATTTAAAAG CTTTTAATCC TCGGAATTCT 
GATTACATAC GAAATAAATA CAAGAAAAAG CTGGAACAAT TTGTGGAGCG ATGTGAACTT 
ATAACATACC TTGGAGACAC AATGACTCGC AAGTACAAAG AACCCCAAAG AAGGCCCATT 
GATTTTGACT TTAAGATGCA GTCTTTCTTG TCATTGAAAG GCATGGACCC AACCTGCATT 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031757751.1
CDS	109..5571
Translation	MASQERPTFADIFDEDEAQQSFLMSKPTCFLIVGKPGTGKATLGKKISQAWNSIFIEAKELITDHIKRGTDQGNKMQALLHQGLSIPEELVTHLILDKINSPEVAHLGYVLCGFPSLCEEYVKIPQQIELIKNFKLTPDIIINIKCPDTDLGKRLCGLKQDPNTGIIYQREDWDPALKEKQKKQQVGEEGEEEEEEEEEEELEEEQEEEMSLTEKLVYRPEDFPENVKERIKLFKDILLRPVEDLMIDHDPHYLIELDGNMKPQKIFETVLSRLVSLGLRLGSVVMKLQNSEEEETLQETDTDQLLRLLASSSMIAPRYRWRRSRWGQTCPVALKEGFIKRGLVEFAVSFLDKMYLMSSEEALRKFMQNPRQYLLPPMPLSPCKVAVVGPKCSGKTTLCNFIANKYAGKVLDMSVLIKPFAEEAKKNFIEKAVEEATELAIKTVRMKLQEERILKEQDKPEETEGIEELEYQKEMPETLAAEVLEEITADHPEVLEIVAEAVKAASETPVTITPDLYMAALEQAINKLHEENKERFTSDPPAGGWVLDNFPNSPNCWLAMADRGFLPDTVICLRDSADNGKYLLNRLYLMNEQDINDNIIQRLQKERARKLQEEEEARKEQLEIMRLQQEEKQRQEEQQESSEDGMEMSTELSEQPSHIVSEQVISSVPSPNEEVQSNMSELEAGDKTLLSNTAPLPISSKDEHNLAEPQPQPEIILPEVPEGGFPDVPEMEPLKEFITKFNEGWILIEPILNDAGNPILTYLEIKEKSPETMLQETISSMDKPFKYYGWEMTSEDIDEEAEDMQAELDSEEQEQEENENESGGEEEEEDEDRIRERKRNYGDSKHFCPVALKDNFVLYPGESEYAAVYREKLYYCSTPEARDKFLDNPENYIINKEPLKAPPLRVLLIGNSGAGKTVCARWLAEKLGIFHIQFREYLQEIMLSKLQRKIGPQYAEEVKEYPNEETPLMELENSDLAIAEENTAEEEVVLTDEEEAVKSYLEDASPLPAELLDQIVTDWWSEEPFRSTGFILDGFPNTAEETQYLGEKGFFPDIAVFLDVEESAICDRLLPPRLAKWKERQMRKEERKQKLKALKKQIRDDQIAKRRAELMAEQNKMKEEKENQKNPDEQEEEEEEEGDEGDDIELILSEEFPDEEEDDEEEEESEYDAVERMKSEIGEKCEAEAESLETVKDELQRLKIPVVTINGGRKRHIVHYQLYEKLRNLVENRESLFEKSYPISLAVANKMLQMSYKQPSVFGRWDPVKLSQGEVIKPFFTQENPGFPLLYRQYIYFFSTKENRNLFMKNPIKYIQQAKPKPSVPIRIAVVGPPKSGKTSVAKMFTSLYGLKRLSIGDAIRSVLENQPNTELATELNSILRKGLVLPDDLSVKCLEVALMDLTSNTNGVVFDGFPVTKHQVDLLEACSIIPIKIFELQAPIKEVLKRGLEDKKNSNRPYPVHDSAQILVVRNSCYKQEVILTKEHYSKLHQNWCEVDSLRSKWWVSNKITGEVQKSINQIQSYLERIKEGMAAGIADFCITPQELVSRLGEFGQYCPVCLAQRGELVDCSGNPSLQFAAEFRGHYYKMASQKELDAFLKSPELYVPPLAPRPIPPPELLPKTLTVADVKAKFPKNAEMKGYCPVTYVDGNKRYEALVPGHIENAVEYRDKIYIFETKEKLQKFMRLPEKYYNQKLPCKLPPKIEPVLLTSLPLTGYLEQGAATALIKAMNDVGCLKPKYPYLNIKQSALLYIAFHLKAFNPRNSDYIRNKYKKKLEQFVERCELITYLGDTMTRKYKEPQRRPIDFDFKMQSFLSLKGMDPTCI

Target ORF information:

RefSeq Version	XM_031901891.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis adenylate kinase 9 (ak9), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031901891.1

ORF Insert Sequence:

ATGGCTTCTC AAGAAAGGCC AACTTTTGCA GACATATTTG ATGAAGATGA AGCACAACAA 
TCCTTTCTCA TGTCCAAACC CACTTGCTTT CTCATTGTTG GAAAACCTGG AACTGGAAAA 
GCTACTTTAG GGAAGAAGAT TTCTCAGGCA TGGAACTCTA TTTTTATTGA AGCTAAAGAA 
CTGATTACTG ATCATATTAA AAGAGGGACG GATCAGGGCA ATAAGATGCA GGCGCTCCTT 
CATCAGGGCT TGAGTATTCC TGAGGAACTA GTAACACATT TAATTTTGGA CAAGATAAAT 
TCTCCAGAGG TTGCTCATCT TGGATATGTG CTATGCGGGT TTCCCTCTCT ATGTGAAGAA 
TATGTAAAGA TCCCCCAACA AATTGAACTG ATTAAAAATT TCAAGTTAAC CCCAGATATT 
ATAATTAATA TTAAGTGCCC AGACACAGAT CTAGGAAAAA GACTGTGTGG CCTAAAGCAA 
GATCCAAATA CAGGCATTAT TTATCAAAGA GAAGATTGGG ATCCTGCTCT GAAAGAAAAA 
CAGAAGAAGC AGCAAGTAGG AGAAGAGGGA GAAGAAGAGG AGGAGGAGGA GGAGGAGGAA 
GAGTTGGAAG AAGAACAGGA AGAAGAAATG TCGTTAACAG AAAAGCTTGT TTATAGGCCT 
GAAGACTTTC CTGAAAATGT TAAAGAACGC ATAAAGCTAT TCAAAGATAT TCTTCTGCGC 
CCTGTGGAGG ATCTAATGAT TGACCATGAT CCTCACTATC TCATAGAGCT GGATGGAAAT 
ATGAAACCAC AGAAAATTTT TGAAACAGTA TTATCTCGTC TTGTATCTTT GGGGCTTCGA 
CTTGGATCAG TTGTGATGAA GTTACAGAAT TCCGAGGAAG AAGAAACACT GCAGGAAACA 
GATACTGATC AACTCTTGCG ATTACTGGCA TCTAGCTCTA TGATTGCTCC AAGATATAGG 
TGGCGCAGAA GCAGATGGGG TCAGACATGT CCTGTTGCTT TAAAGGAAGG ATTTATTAAA 
AGAGGACTTG TGGAGTTTGC TGTTAGTTTC CTGGATAAAA TGTATTTGAT GTCTTCAGAA 
GAGGCACTTA GAAAATTTAT GCAGAATCCT CGTCAGTATC TTCTCCCTCC CATGCCACTT 
TCACCTTGTA AAGTAGCAGT GGTTGGACCG AAGTGCTCTG GAAAAACCAC CCTTTGCAAT 
TTCATAGCCA ATAAATATGC AGGAAAGGTC CTTGATATGT CTGTGCTTAT AAAACCATTT 
GCTGAAGAAG CTAAGAAAAA TTTTATAGAA AAAGCGGTGG AGGAAGCAAC TGAACTGGCT 
ATTAAAACTG TGAGAATGAA GTTACAAGAA GAGAGGATTT TAAAAGAACA AGACAAACCA 
GAAGAAACAG AGGGGATAGA AGAGCTGGAG TATCAAAAGG AAATGCCAGA AACCTTAGCT 
GCAGAGGTAT TGGAAGAAAT TACTGCTGAT CATCCAGAGG TCTTAGAAAT AGTGGCTGAA 
GCTGTAAAAG CTGCAAGTGA GACTCCTGTG ACCATTACTC CAGATCTTTA TATGGCTGCA 
CTAGAACAAG CAATAAACAA GTTGCATGAA GAAAATAAAG AAAGATTTAC AAGTGATCCA 
CCAGCTGGGG GATGGGTACT GGATAATTTT CCTAATTCTC CAAACTGCTG GTTAGCAATG 
GCGGACAGGG GATTTCTCCC AGACACTGTC ATTTGTTTAA GAGATTCAGC AGATAATGGA 
AAGTACTTGC TGAACAGACT GTACCTTATG AATGAACAAG ACATTAATGA TAATATCATT 
CAAAGACTTC AGAAAGAAAG AGCAAGGAAG CTACAAGAAG AGGAGGAGGC AAGGAAAGAA 
CAGCTAGAAA TAATGAGATT GCAACAAGAG GAAAAACAGA GGCAAGAAGA GCAGCAGGAA 
AGCTCAGAGG ATGGCATGGA AATGTCTACA GAATTGAGTG AACAGCCTTC TCACATTGTT 
TCAGAGCAAG TTATATCTTC GGTGCCTTCT CCAAATGAAG AAGTTCAAAG TAACATGTCA 
GAACTAGAGG CTGGTGACAA GACTCTTCTA AGTAACACAG CTCCACTGCC GATTTCTTCC 
AAGGATGAGC ACAACCTGGC AGAGCCCCAA CCACAGCCAG AAATTATTTT GCCAGAAGTC 
CCAGAGGGCG GATTCCCAGA TGTGCCAGAA ATGGAGCCAC TGAAAGAGTT TATTACCAAG 
TTTAATGAAG GATGGATTCT GATAGAACCA ATACTTAATG ATGCAGGAAA CCCAATTCTC 
ACATATTTGG AGATAAAAGA GAAATCACCT GAGACAATGC TACAAGAAAC CATCAGTTCT 
ATGGATAAAC CATTTAAGTA TTATGGCTGG GAAATGACAT CAGAAGACAT AGATGAAGAA 
GCTGAAGACA TGCAAGCCGA GCTAGATTCT GAGGAACAAG AACAGGAGGA GAATGAAAAT 
GAGAGTGGAG GAGAAGAAGA AGAAGAAGAT GAAGATCGCA TTCGTGAAAG GAAGAGAAAC 
TATGGGGATT CCAAACACTT TTGTCCAGTG GCCCTAAAAG ATAATTTTGT GCTGTATCCT 
GGAGAATCTG AATATGCAGC AGTATACAGG GAGAAGCTTT ATTACTGTTC AACTCCTGAA 
GCAAGGGACA AGTTCCTAGA CAATCCTGAG AATTATATAA TTAACAAAGA ACCTTTAAAG 
GCTCCACCAT TGAGAGTGCT TTTAATTGGA AACAGTGGTG CTGGCAAAAC AGTTTGTGCA 
AGGTGGTTGG CAGAAAAATT AGGCATTTTC CACATACAGT TTAGAGAGTA CCTGCAAGAG 
ATAATGCTTT CCAAACTTCA AAGGAAAATT GGACCACAGT ATGCTGAGGA AGTAAAAGAG 
TATCCAAATG AGGAAACTCC TCTAATGGAA CTAGAAAATA GTGACTTAGC TATTGCCGAA 
GAAAACACTG CAGAGGAAGA GGTGGTTTTA ACAGATGAAG AAGAAGCAGT CAAATCATAT 
TTAGAAGATG CATCCCCATT GCCAGCTGAA CTGCTGGATC AGATTGTAAC AGACTGGTGG 
TCAGAAGAAC CTTTTCGCTC AACTGGGTTT ATATTAGATG GCTTTCCTAA TACAGCAGAA 
GAGACACAGT ACTTAGGGGA GAAGGGCTTT TTCCCAGACA TTGCTGTCTT TCTTGATGTA 
GAAGAAAGTG CCATTTGCGA CCGCCTTCTT CCTCCACGTT TGGCAAAATG GAAAGAACGT 
CAAATGAGAA AAGAGGAGCG CAAACAAAAA TTAAAAGCAC TCAAGAAACA AATAAGGGAC 
GATCAGATTG CAAAGAGAAG GGCTGAATTA ATGGCTGAAC AAAATAAAAT GAAAGAAGAA 
AAAGAGAATC AAAAAAACCC AGATGAACAG GAGGAGGAGG AGGAGGAAGA AGGTGATGAA 
GGGGATGATA TTGAACTCAT TCTTTCAGAA GAGTTTCCAG ATGAGGAAGA AGATGATGAG 
GAAGAAGAGG AGTCAGAATA TGATGCAGTT GAAAGAATGA AAAGTGAAAT AGGGGAGAAG 
TGTGAAGCTG AAGCAGAGTC TCTAGAAACA GTGAAGGATG AGCTTCAGAG ACTTAAGATC 
CCTGTTGTGA CAATAAATGG AGGCCGGAAA CGACATATTG TGCACTACCA GTTATATGAG 
AAGCTAAGAA ATCTTGTAGA AAACCGTGAA AGCCTCTTTG AAAAAAGCTA CCCCATTAGC 
CTTGCTGTTG CTAATAAGAT GCTCCAAATG TCTTACAAAC AGCCTAGTGT CTTTGGACGG 
TGGGACCCAG TCAAGCTAAG CCAAGGAGAG GTAATAAAAC CATTTTTCAC CCAAGAAAAT 
CCCGGCTTTC CATTACTATA CCGCCAATAT ATTTACTTCT TCTCGACAAA GGAAAACAGG 
AACTTATTTA TGAAAAATCC TATCAAATAC ATCCAGCAGG CAAAACCCAA GCCATCAGTG 
CCAATAAGAA TAGCTGTTGT TGGGCCTCCT AAATCTGGCA AGACTTCAGT TGCTAAGATG 
TTTACGAGTT TATATGGGCT GAAAAGACTT TCAATTGGAG ATGCCATTCG CTCTGTTTTG 
GAAAATCAGC CAAACACTGA ATTGGCAACA GAATTAAACT CTATACTTCG GAAAGGGTTG 
GTTTTGCCTG ATGATCTTTC TGTTAAGTGT CTAGAAGTAG CTTTGATGGA TTTAACTTCT 
AATACAAATG GAGTTGTTTT TGATGGTTTT CCTGTTACCA AACATCAAGT AGATCTTCTG 
GAAGCCTGCA GTATAATTCC TATAAAAATA TTTGAGCTGC AAGCCCCTAT AAAGGAAGTG 
TTAAAAAGAG GACTGGAAGA CAAGAAGAAC TCTAACAGGC CTTACCCTGT GCATGATAGT 
GCTCAAATTC TTGTTGTACG CAACTCCTGT TATAAACAAG AAGTCATTCT GACCAAAGAA 
CACTACTCAA AGCTGCATCA GAACTGGTGT GAAGTTGATT CTTTAAGGAG TAAATGGTGG 
GTTTCCAACA AAATTACTGG AGAAGTTCAG AAAAGTATCA ATCAAATACA GTCCTACTTG 
GAGAGAATAA AAGAAGGTAT GGCAGCTGGC ATTGCTGACT TCTGCATCAC TCCTCAGGAG 
CTAGTTTCTC GGCTGGGTGA GTTTGGACAA TACTGCCCTG TGTGTCTAGC ACAAAGGGGA 
GAGCTTGTTG ACTGTTCTGG AAATCCATCC CTGCAGTTTG CTGCAGAATT CCGGGGACAC 
TATTACAAGA TGGCTTCACA GAAGGAGCTG GATGCTTTCT TGAAAAGTCC TGAATTATAT 
GTGCCACCTC TTGCACCGCG CCCAATCCCT CCTCCTGAGC TGTTACCAAA AACGCTAACT 
GTGGCTGATG TGAAGGCAAA ATTTCCTAAA AATGCAGAAA TGAAGGGGTA TTGCCCAGTT 
ACCTATGTTG ATGGTAATAA AAGATACGAA GCACTGGTCC CTGGTCATAT TGAGAATGCA 
GTAGAATACA GAGATAAGAT TTATATTTTT GAGACCAAGG AAAAGCTACA GAAGTTCATG 
AGACTTCCAG AGAAATACTA CAATCAGAAG CTTCCATGCA AACTTCCACC AAAAATAGAA 
CCTGTGCTTT TGACTTCACT TCCATTAACT GGATACCTTG AGCAGGGAGC TGCAACTGCT 
TTAATAAAAG CAATGAATGA TGTAGGCTGC CTAAAGCCCA AGTATCCATA TCTAAACATC 
AAACAATCTG CTCTGCTCTA TATTGCTTTT CATTTAAAAG CTTTTAATCC TCGGAATTCT 
GATTACATAC GAAATAAATA CAAGAAAAAG CTGGAACAAT TTGTGGAGCG ATGTGAACTT 
ATAACATACC TTGGAGACAC AATGACTCGC AAGTACAAAG AACCCCAAAG AAGGCCCATT 
GATTTTGACT TTAAGATGCA GTCTTTCTTG TCATTGAAAG GCATGGACCC AACCTGCATT 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ak9 ( XM_031901892.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_031757752.1 Xenopus tropicalis adenylate kinase 9 (ak9), transcript variant X2, mRNA.

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CloneID	OXa58178
Clone ID Related Accession (Same CDS sequence)	XM_031901892.1
Accession Version	XM_031901892.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5454bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1576512000000
Organism	Xenopus tropicalis(tropical clawed frog)
Product	adenylate kinase 9 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030681.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTTCTC AAGAAAGGCC AACTTTTGCA GACATATTTG ATGAAGATGA AGCACAACAA 
TCCTTTCTCA TGTCCAAACC CACTTGCTTT CTCATTGTTG GAAAACCTGG AACTGGAAAA 
GCTACTTTAG GGAAGAAGAT TTCTCAGGCA TGGAACTCTA TTTTTATTGA AGCTAAAGAA 
CTGATTACTG ATCATATTAA AAGAGGGACG GATCAGGGCA ATAAGATGCA GGCGCTCCTT 
CATCAGGGCT TGAGTATTCC TGAGGAACTA GTAACACATT TAATTTTGGA CAAGATAAAT 
TCTCCAGAGG TTGCTCATCT TGGATATGTG CTATGCGGGT TTCCCTCTCT ATGTGAAGAA 
TATGTAAAGA TCCCCCAACA AATTGAACTG ATTAAAAATT TCAAGTTAAC CCCAGATATT 
ATAATTAATA TTAAGTGCCC AGACACAGAT CTAGGAAAAA GACTGTGTGG CCTAAAGCAA 
GATCCAAATA CAGGCATTAT TTATCAAAGA GAAGATTGGG ATCCTGCTCT GAAAGAAAAA 
CAGAAGAAGC AGCAAGTAGG AGAAGAGGGA GAAGAAGAGG AGGAGGAGGA GGAGGAGGAA 
GAGTTGGAAG AAGAACAGGA AGAAGAAATG TCGTTAACAG AAAAGCTTGT TTATAGGCCT 
GAAGACTTTC CTGAAAATGT TAAAGAACGC ATAAAGCTAT TCAAAGATAT TCTTCTGCGC 
CCTGTGGAGG ATCTAATGAT TGACCATGAT CCTCACTATC TCATAGAGCT GGATGGAAAT 
ATGAAACCAC AGAAAATTTT TGAAACAGTA TTATCTCGTC TTGTATCTTT GGGGCTTCGA 
CTTGGATCAG TTGTGATGAA GTTACAGAAT TCCGAGGAAG AAGAAACACT GCAGGAAACA 
GATACTGATC AACTCTTGCG ATTACTGGCA TCTAGCTCTA TGATTGCTCC AAGATATAGG 
TGGCGCAGAA GCAGATGGGG TCAGACATGT CCTGTTGCTT TAAAGGAAGG ATTTATTAAA 
AGAGGACTTG TGGAGTTTGC TGTTAGTTTC CTGGATAAAA TGTATTTGAT GTCTTCAGAA 
GAGGCACTTA GAAAATTTAT GCAGAATCCT CGTCAGTATC TTCTCCCTCC CATGCCACTT 
TCACCTTGTA AAGTAGCAGT GGTTGGACCG AAGTGCTCTG GAAAAACCAC CCTTTGCAAT 
TTCATAGCCA ATAAATATGC AGGAAAGGTC CTTGATATGT CTGTGCTTAT AAAACCATTT 
GCTGAAGAAG CTAAGAAAAA TTTTATAGAA AAAGCGGTGG AGGAAGCAAC TGAACTGGCT 
ATTAAAACTG TGAGAATGAA GTTACAAGAA GAGAGGATTT TAAAAGAACA AGAAGAAACA 
GAGGGGATAG AAGAGCTGGA GTATCAAAAG GAAATGCCAG AAACCTTAGC TGCAGAGGTA 
TTGGAAGAAA TTACTGCTGA TCATCCAGAG GTCTTAGAAA TAGTGGCTGA AGCTGTAAAA 
GCTGCAAGTG AGACTCCTGT GACCATTACT CCAGATCTTT ATATGGCTGC ACTAGAACAA 
GCAATAAACA AGTTGCATGA AGAAAATAAA GAAAGATTTA CAAGTGATCC ACCAGCTGGG 
GGATGGGTAC TGGATAATTT TCCTAATTCT CCAAACTGCT GGTTAGCAAT GGCGGACAGG 
GGATTTCTCC CAGACACTGT CATTTGTTTA AGAGATTCAG CAGATAATGG AAAGTACTTG 
CTGAACAGAC TGTACCTTAT GAATGAACAA GACATTAATG ATAATATCAT TCAAAGACTT 
CAGAAAGAAA GAGCAAGGAA GCTACAAGAA GAGGAGGAGG CAAGGAAAGA ACAGCTAGAA 
ATAATGAGAT TGCAACAAGA GGAAAAACAG AGGCAAGAAG AGCAGCAGGA AAGCTCAGAG 
GATGGCATGG AAATGTCTAC AGAATTGAGT GAACAGCCTT CTCACATTGT TTCAGAGCAA 
GTTATATCTT CGGTGCCTTC TCCAAATGAA GAAGTTCAAA GTAACATGTC AGAACTAGAG 
GCTGGTGACA AGACTCTTCT AAGTAACACA GCTCCACTGC CGATTTCTTC CAAGGATGAG 
CACAACCTGG CAGAGCCCCA ACCACAGCCA GAAATTATTT TGCCAGAAGT CCCAGAGGGC 
GGATTCCCAG ATGTGCCAGA AATGGAGCCA CTGAAAGAGT TTATTACCAA GTTTAATGAA 
GGATGGATTC TGATAGAACC AATACTTAAT GATGCAGGAA ACCCAATTCT CACATATTTG 
GAGATAAAAG AGAAATCACC TGAGACAATG CTACAAGAAA CCATCAGTTC TATGGATAAA 
CCATTTAAGT ATTATGGCTG GGAAATGACA TCAGAAGACA TAGATGAAGA AGCTGAAGAC 
ATGCAAGCCG AGCTAGATTC TGAGGAACAA GAACAGGAGG AGAATGAAAA TGAGAGTGGA 
GGAGAAGAAG AAGAAGAAGA TGAAGATCGC ATTCGTGAAA GGAAGAGAAA CTATGGGGAT 
TCCAAACACT TTTGTCCAGT GGCCCTAAAA GATAATTTTG TGCTGTATCC TGGAGAATCT 
GAATATGCAG CAGTATACAG GGAGAAGCTT TATTACTGTT CAACTCCTGA AGCAAGGGAC 
AAGTTCCTAG ACAATCCTGA GAATTATATA ATTAACAAAG AACCTTTAAA GGCTCCACCA 
TTGAGAGTGC TTTTAATTGG AAACAGTGGT GCTGGCAAAA CAGTTTGTGC AAGGTGGTTG 
GCAGAAAAAT TAGGCATTTT CCACATACAG TTTAGAGAGT ACCTGCAAGA GATAATGCTT 
TCCAAACTTC AAAGGAAAAT TGGACCACAG TATGCTGAGG AAGTAAAAGA GTATCCAAAT 
GAGGAAACTC CTCTAATGGA ACTAGAAAAT AGTGACTTAG CTATTGCCGA AGAAAACACT 
GCAGAGGAAG AGGTGGTTTT AACAGATGAA GAAGAAGCAG TCAAATCATA TTTAGAAGAT 
GCATCCCCAT TGCCAGCTGA ACTGCTGGAT CAGATTGTAA CAGACTGGTG GTCAGAAGAA 
CCTTTTCGCT CAACTGGGTT TATATTAGAT GGCTTTCCTA ATACAGCAGA AGAGACACAG 
TACTTAGGGG AGAAGGGCTT TTTCCCAGAC ATTGCTGTCT TTCTTGATGT AGAAGAAAGT 
GCCATTTGCG ACCGCCTTCT TCCTCCACGT TTGGCAAAAT GGAAAGAACG TCAAATGAGA 
AAAGAGGAGC GCAAACAAAA ATTAAAAGCA CTCAAGAAAC AAATAAGGGA CGATCAGATT 
GCAAAGAGAA GGGCTGAATT AATGGCTGAA CAAAATAAAA TGAAAGAAGA AAAAGAGAAT 
CAAAAAAACC CAGATGAACA GGAGGAGGAG GAGGAGGAAG AAGGTGATGA AGGGGATGAT 
ATTGAACTCA TTCTTTCAGA AGAGTTTCCA GATGAGGAAG AAGATGATGA GGAAGAAGAG 
GAGTCAGAAT ATGATGCAGT TGAAAGAATG AAAAGTGAAA TAGGGGAGAA GTGTGAAGCT 
GAAGCAGAGT CTCTAGAAAC AGTGAAGGAT GAGCTTCAGA GACTTAAGAT CCCTGTTGTG 
ACAATAAATG GAGGCCGGAA ACGACATATT GTGCACTACC AGTTATATGA GAAGCTAAGA 
AATCTTGTAG AAAACCGTGA AAGCCTCTTT GAAAAAAGCT ACCCCATTAG CCTTGCTGTT 
GCTAATAAGA TGCTCCAAAT GTCTTACAAA CAGCCTAGTG TCTTTGGACG GTGGGACCCA 
GTCAAGCTAA GCCAAGGAGA GGTAATAAAA CCATTTTTCA CCCAAGAAAA TCCCGGCTTT 
CCATTACTAT ACCGCCAATA TATTTACTTC TTCTCGACAA AGGAAAACAG GAACTTATTT 
ATGAAAAATC CTATCAAATA CATCCAGCAG GCAAAACCCA AGCCATCAGT GCCAATAAGA 
ATAGCTGTTG TTGGGCCTCC TAAATCTGGC AAGACTTCAG TTGCTAAGAT GTTTACGAGT 
TTATATGGGC TGAAAAGACT TTCAATTGGA GATGCCATTC GCTCTGTTTT GGAAAATCAG 
CCAAACACTG AATTGGCAAC AGAATTAAAC TCTATACTTC GGAAAGGGTT GGTTTTGCCT 
GATGATCTTT CTGTTAAGTG TCTAGAAGTA GCTTTGATGG ATTTAACTTC TAATACAAAT 
GGAGTTGTTT TTGATGGTTT TCCTGTTACC AAACATCAAG TAGATCTTCT GGAAGCCTGC 
AGTATAATTC CTATAAAAAT ATTTGAGCTG CAAGCCCCTA TAAAGGAAGT GTTAAAAAGA 
GGACTGGAAG ACAAGAAGAA CTCTAACAGG CCTTACCCTG TGCATGATAG TGCTCAAATT 
CTTGTTGTAC GCAACTCCTG TTATAAACAA GAAGTCATTC TGACCAAAGA ACACTACTCA 
AAGCTGCATC AGAACTGGTG TGAAGTTGAT TCTTTAAGGA GTAAATGGTG GGTTTCCAAC 
AAAATTACTG GAGAAGTTCA GAAAAGTATC AATCAAATAC AGTCCTACTT GGAGAGAATA 
AAAGAAGGTA TGGCAGCTGG CATTGCTGAC TTCTGCATCA CTCCTCAGGA GCTAGTTTCT 
CGGCTGGGTG AGTTTGGACA ATACTGCCCT GTGTGTCTAG CACAAAGGGG AGAGCTTGTT 
GACTGTTCTG GAAATCCATC CCTGCAGTTT GCTGCAGAAT TCCGGGGACA CTATTACAAG 
ATGGCTTCAC AGAAGGAGCT GGATGCTTTC TTGAAAAGTC CTGAATTATA TGTGCCACCT 
CTTGCACCGC GCCCAATCCC TCCTCCTGAG CTGTTACCAA AAACGCTAAC TGTGGCTGAT 
GTGAAGGCAA AATTTCCTAA AAATGCAGAA ATGAAGGGGT ATTGCCCAGT TACCTATGTT 
GATGGTAATA AAAGATACGA AGCACTGGTC CCTGGTCATA TTGAGAATGC AGTAGAATAC 
AGAGATAAGA TTTATATTTT TGAGACCAAG GAAAAGCTAC AGAAGTTCAT GAGACTTCCA 
GAGAAATACT ACAATCAGAA GCTTCCATGC AAACTTCCAC CAAAAATAGA ACCTGTGCTT 
TTGACTTCAC TTCCATTAAC TGGATACCTT GAGCAGGGAG CTGCAACTGC TTTAATAAAA 
GCAATGAATG ATGTAGGCTG CCTAAAGCCC AAGTATCCAT ATCTAAACAT CAAACAATCT 
GCTCTGCTCT ATATTGCTTT TCATTTAAAA GCTTTTAATC CTCGGAATTC TGATTACATA 
CGAAATAAAT ACAAGAAAAA GCTGGAACAA TTTGTGGAGC GATGTGAACT TATAACATAC 
CTTGGAGACA CAATGACTCG CAAGTACAAA GAACCCCAAA GAAGGCCCAT TGATTTTGAC 
TTTAAGATGC AGTCTTTCTT GTCATTGAAA GGCATGGACC CAACCTGCAT TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031757752.1
CDS	109..5562
Translation	MASQERPTFADIFDEDEAQQSFLMSKPTCFLIVGKPGTGKATLGKKISQAWNSIFIEAKELITDHIKRGTDQGNKMQALLHQGLSIPEELVTHLILDKINSPEVAHLGYVLCGFPSLCEEYVKIPQQIELIKNFKLTPDIIINIKCPDTDLGKRLCGLKQDPNTGIIYQREDWDPALKEKQKKQQVGEEGEEEEEEEEEEELEEEQEEEMSLTEKLVYRPEDFPENVKERIKLFKDILLRPVEDLMIDHDPHYLIELDGNMKPQKIFETVLSRLVSLGLRLGSVVMKLQNSEEEETLQETDTDQLLRLLASSSMIAPRYRWRRSRWGQTCPVALKEGFIKRGLVEFAVSFLDKMYLMSSEEALRKFMQNPRQYLLPPMPLSPCKVAVVGPKCSGKTTLCNFIANKYAGKVLDMSVLIKPFAEEAKKNFIEKAVEEATELAIKTVRMKLQEERILKEQEETEGIEELEYQKEMPETLAAEVLEEITADHPEVLEIVAEAVKAASETPVTITPDLYMAALEQAINKLHEENKERFTSDPPAGGWVLDNFPNSPNCWLAMADRGFLPDTVICLRDSADNGKYLLNRLYLMNEQDINDNIIQRLQKERARKLQEEEEARKEQLEIMRLQQEEKQRQEEQQESSEDGMEMSTELSEQPSHIVSEQVISSVPSPNEEVQSNMSELEAGDKTLLSNTAPLPISSKDEHNLAEPQPQPEIILPEVPEGGFPDVPEMEPLKEFITKFNEGWILIEPILNDAGNPILTYLEIKEKSPETMLQETISSMDKPFKYYGWEMTSEDIDEEAEDMQAELDSEEQEQEENENESGGEEEEEDEDRIRERKRNYGDSKHFCPVALKDNFVLYPGESEYAAVYREKLYYCSTPEARDKFLDNPENYIINKEPLKAPPLRVLLIGNSGAGKTVCARWLAEKLGIFHIQFREYLQEIMLSKLQRKIGPQYAEEVKEYPNEETPLMELENSDLAIAEENTAEEEVVLTDEEEAVKSYLEDASPLPAELLDQIVTDWWSEEPFRSTGFILDGFPNTAEETQYLGEKGFFPDIAVFLDVEESAICDRLLPPRLAKWKERQMRKEERKQKLKALKKQIRDDQIAKRRAELMAEQNKMKEEKENQKNPDEQEEEEEEEGDEGDDIELILSEEFPDEEEDDEEEEESEYDAVERMKSEIGEKCEAEAESLETVKDELQRLKIPVVTINGGRKRHIVHYQLYEKLRNLVENRESLFEKSYPISLAVANKMLQMSYKQPSVFGRWDPVKLSQGEVIKPFFTQENPGFPLLYRQYIYFFSTKENRNLFMKNPIKYIQQAKPKPSVPIRIAVVGPPKSGKTSVAKMFTSLYGLKRLSIGDAIRSVLENQPNTELATELNSILRKGLVLPDDLSVKCLEVALMDLTSNTNGVVFDGFPVTKHQVDLLEACSIIPIKIFELQAPIKEVLKRGLEDKKNSNRPYPVHDSAQILVVRNSCYKQEVILTKEHYSKLHQNWCEVDSLRSKWWVSNKITGEVQKSINQIQSYLERIKEGMAAGIADFCITPQELVSRLGEFGQYCPVCLAQRGELVDCSGNPSLQFAAEFRGHYYKMASQKELDAFLKSPELYVPPLAPRPIPPPELLPKTLTVADVKAKFPKNAEMKGYCPVTYVDGNKRYEALVPGHIENAVEYRDKIYIFETKEKLQKFMRLPEKYYNQKLPCKLPPKIEPVLLTSLPLTGYLEQGAATALIKAMNDVGCLKPKYPYLNIKQSALLYIAFHLKAFNPRNSDYIRNKYKKKLEQFVERCELITYLGDTMTRKYKEPQRRPIDFDFKMQSFLSLKGMDPTCI

Target ORF information:

RefSeq Version	XM_031901892.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis adenylate kinase 9 (ak9), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031901892.1

ORF Insert Sequence:

ATGGCTTCTC AAGAAAGGCC AACTTTTGCA GACATATTTG ATGAAGATGA AGCACAACAA 
TCCTTTCTCA TGTCCAAACC CACTTGCTTT CTCATTGTTG GAAAACCTGG AACTGGAAAA 
GCTACTTTAG GGAAGAAGAT TTCTCAGGCA TGGAACTCTA TTTTTATTGA AGCTAAAGAA 
CTGATTACTG ATCATATTAA AAGAGGGACG GATCAGGGCA ATAAGATGCA GGCGCTCCTT 
CATCAGGGCT TGAGTATTCC TGAGGAACTA GTAACACATT TAATTTTGGA CAAGATAAAT 
TCTCCAGAGG TTGCTCATCT TGGATATGTG CTATGCGGGT TTCCCTCTCT ATGTGAAGAA 
TATGTAAAGA TCCCCCAACA AATTGAACTG ATTAAAAATT TCAAGTTAAC CCCAGATATT 
ATAATTAATA TTAAGTGCCC AGACACAGAT CTAGGAAAAA GACTGTGTGG CCTAAAGCAA 
GATCCAAATA CAGGCATTAT TTATCAAAGA GAAGATTGGG ATCCTGCTCT GAAAGAAAAA 
CAGAAGAAGC AGCAAGTAGG AGAAGAGGGA GAAGAAGAGG AGGAGGAGGA GGAGGAGGAA 
GAGTTGGAAG AAGAACAGGA AGAAGAAATG TCGTTAACAG AAAAGCTTGT TTATAGGCCT 
GAAGACTTTC CTGAAAATGT TAAAGAACGC ATAAAGCTAT TCAAAGATAT TCTTCTGCGC 
CCTGTGGAGG ATCTAATGAT TGACCATGAT CCTCACTATC TCATAGAGCT GGATGGAAAT 
ATGAAACCAC AGAAAATTTT TGAAACAGTA TTATCTCGTC TTGTATCTTT GGGGCTTCGA 
CTTGGATCAG TTGTGATGAA GTTACAGAAT TCCGAGGAAG AAGAAACACT GCAGGAAACA 
GATACTGATC AACTCTTGCG ATTACTGGCA TCTAGCTCTA TGATTGCTCC AAGATATAGG 
TGGCGCAGAA GCAGATGGGG TCAGACATGT CCTGTTGCTT TAAAGGAAGG ATTTATTAAA 
AGAGGACTTG TGGAGTTTGC TGTTAGTTTC CTGGATAAAA TGTATTTGAT GTCTTCAGAA 
GAGGCACTTA GAAAATTTAT GCAGAATCCT CGTCAGTATC TTCTCCCTCC CATGCCACTT 
TCACCTTGTA AAGTAGCAGT GGTTGGACCG AAGTGCTCTG GAAAAACCAC CCTTTGCAAT 
TTCATAGCCA ATAAATATGC AGGAAAGGTC CTTGATATGT CTGTGCTTAT AAAACCATTT 
GCTGAAGAAG CTAAGAAAAA TTTTATAGAA AAAGCGGTGG AGGAAGCAAC TGAACTGGCT 
ATTAAAACTG TGAGAATGAA GTTACAAGAA GAGAGGATTT TAAAAGAACA AGAAGAAACA 
GAGGGGATAG AAGAGCTGGA GTATCAAAAG GAAATGCCAG AAACCTTAGC TGCAGAGGTA 
TTGGAAGAAA TTACTGCTGA TCATCCAGAG GTCTTAGAAA TAGTGGCTGA AGCTGTAAAA 
GCTGCAAGTG AGACTCCTGT GACCATTACT CCAGATCTTT ATATGGCTGC ACTAGAACAA 
GCAATAAACA AGTTGCATGA AGAAAATAAA GAAAGATTTA CAAGTGATCC ACCAGCTGGG 
GGATGGGTAC TGGATAATTT TCCTAATTCT CCAAACTGCT GGTTAGCAAT GGCGGACAGG 
GGATTTCTCC CAGACACTGT CATTTGTTTA AGAGATTCAG CAGATAATGG AAAGTACTTG 
CTGAACAGAC TGTACCTTAT GAATGAACAA GACATTAATG ATAATATCAT TCAAAGACTT 
CAGAAAGAAA GAGCAAGGAA GCTACAAGAA GAGGAGGAGG CAAGGAAAGA ACAGCTAGAA 
ATAATGAGAT TGCAACAAGA GGAAAAACAG AGGCAAGAAG AGCAGCAGGA AAGCTCAGAG 
GATGGCATGG AAATGTCTAC AGAATTGAGT GAACAGCCTT CTCACATTGT TTCAGAGCAA 
GTTATATCTT CGGTGCCTTC TCCAAATGAA GAAGTTCAAA GTAACATGTC AGAACTAGAG 
GCTGGTGACA AGACTCTTCT AAGTAACACA GCTCCACTGC CGATTTCTTC CAAGGATGAG 
CACAACCTGG CAGAGCCCCA ACCACAGCCA GAAATTATTT TGCCAGAAGT CCCAGAGGGC 
GGATTCCCAG ATGTGCCAGA AATGGAGCCA CTGAAAGAGT TTATTACCAA GTTTAATGAA 
GGATGGATTC TGATAGAACC AATACTTAAT GATGCAGGAA ACCCAATTCT CACATATTTG 
GAGATAAAAG AGAAATCACC TGAGACAATG CTACAAGAAA CCATCAGTTC TATGGATAAA 
CCATTTAAGT ATTATGGCTG GGAAATGACA TCAGAAGACA TAGATGAAGA AGCTGAAGAC 
ATGCAAGCCG AGCTAGATTC TGAGGAACAA GAACAGGAGG AGAATGAAAA TGAGAGTGGA 
GGAGAAGAAG AAGAAGAAGA TGAAGATCGC ATTCGTGAAA GGAAGAGAAA CTATGGGGAT 
TCCAAACACT TTTGTCCAGT GGCCCTAAAA GATAATTTTG TGCTGTATCC TGGAGAATCT 
GAATATGCAG CAGTATACAG GGAGAAGCTT TATTACTGTT CAACTCCTGA AGCAAGGGAC 
AAGTTCCTAG ACAATCCTGA GAATTATATA ATTAACAAAG AACCTTTAAA GGCTCCACCA 
TTGAGAGTGC TTTTAATTGG AAACAGTGGT GCTGGCAAAA CAGTTTGTGC AAGGTGGTTG 
GCAGAAAAAT TAGGCATTTT CCACATACAG TTTAGAGAGT ACCTGCAAGA GATAATGCTT 
TCCAAACTTC AAAGGAAAAT TGGACCACAG TATGCTGAGG AAGTAAAAGA GTATCCAAAT 
GAGGAAACTC CTCTAATGGA ACTAGAAAAT AGTGACTTAG CTATTGCCGA AGAAAACACT 
GCAGAGGAAG AGGTGGTTTT AACAGATGAA GAAGAAGCAG TCAAATCATA TTTAGAAGAT 
GCATCCCCAT TGCCAGCTGA ACTGCTGGAT CAGATTGTAA CAGACTGGTG GTCAGAAGAA 
CCTTTTCGCT CAACTGGGTT TATATTAGAT GGCTTTCCTA ATACAGCAGA AGAGACACAG 
TACTTAGGGG AGAAGGGCTT TTTCCCAGAC ATTGCTGTCT TTCTTGATGT AGAAGAAAGT 
GCCATTTGCG ACCGCCTTCT TCCTCCACGT TTGGCAAAAT GGAAAGAACG TCAAATGAGA 
AAAGAGGAGC GCAAACAAAA ATTAAAAGCA CTCAAGAAAC AAATAAGGGA CGATCAGATT 
GCAAAGAGAA GGGCTGAATT AATGGCTGAA CAAAATAAAA TGAAAGAAGA AAAAGAGAAT 
CAAAAAAACC CAGATGAACA GGAGGAGGAG GAGGAGGAAG AAGGTGATGA AGGGGATGAT 
ATTGAACTCA TTCTTTCAGA AGAGTTTCCA GATGAGGAAG AAGATGATGA GGAAGAAGAG 
GAGTCAGAAT ATGATGCAGT TGAAAGAATG AAAAGTGAAA TAGGGGAGAA GTGTGAAGCT 
GAAGCAGAGT CTCTAGAAAC AGTGAAGGAT GAGCTTCAGA GACTTAAGAT CCCTGTTGTG 
ACAATAAATG GAGGCCGGAA ACGACATATT GTGCACTACC AGTTATATGA GAAGCTAAGA 
AATCTTGTAG AAAACCGTGA AAGCCTCTTT GAAAAAAGCT ACCCCATTAG CCTTGCTGTT 
GCTAATAAGA TGCTCCAAAT GTCTTACAAA CAGCCTAGTG TCTTTGGACG GTGGGACCCA 
GTCAAGCTAA GCCAAGGAGA GGTAATAAAA CCATTTTTCA CCCAAGAAAA TCCCGGCTTT 
CCATTACTAT ACCGCCAATA TATTTACTTC TTCTCGACAA AGGAAAACAG GAACTTATTT 
ATGAAAAATC CTATCAAATA CATCCAGCAG GCAAAACCCA AGCCATCAGT GCCAATAAGA 
ATAGCTGTTG TTGGGCCTCC TAAATCTGGC AAGACTTCAG TTGCTAAGAT GTTTACGAGT 
TTATATGGGC TGAAAAGACT TTCAATTGGA GATGCCATTC GCTCTGTTTT GGAAAATCAG 
CCAAACACTG AATTGGCAAC AGAATTAAAC TCTATACTTC GGAAAGGGTT GGTTTTGCCT 
GATGATCTTT CTGTTAAGTG TCTAGAAGTA GCTTTGATGG ATTTAACTTC TAATACAAAT 
GGAGTTGTTT TTGATGGTTT TCCTGTTACC AAACATCAAG TAGATCTTCT GGAAGCCTGC 
AGTATAATTC CTATAAAAAT ATTTGAGCTG CAAGCCCCTA TAAAGGAAGT GTTAAAAAGA 
GGACTGGAAG ACAAGAAGAA CTCTAACAGG CCTTACCCTG TGCATGATAG TGCTCAAATT 
CTTGTTGTAC GCAACTCCTG TTATAAACAA GAAGTCATTC TGACCAAAGA ACACTACTCA 
AAGCTGCATC AGAACTGGTG TGAAGTTGAT TCTTTAAGGA GTAAATGGTG GGTTTCCAAC 
AAAATTACTG GAGAAGTTCA GAAAAGTATC AATCAAATAC AGTCCTACTT GGAGAGAATA 
AAAGAAGGTA TGGCAGCTGG CATTGCTGAC TTCTGCATCA CTCCTCAGGA GCTAGTTTCT 
CGGCTGGGTG AGTTTGGACA ATACTGCCCT GTGTGTCTAG CACAAAGGGG AGAGCTTGTT 
GACTGTTCTG GAAATCCATC CCTGCAGTTT GCTGCAGAAT TCCGGGGACA CTATTACAAG 
ATGGCTTCAC AGAAGGAGCT GGATGCTTTC TTGAAAAGTC CTGAATTATA TGTGCCACCT 
CTTGCACCGC GCCCAATCCC TCCTCCTGAG CTGTTACCAA AAACGCTAAC TGTGGCTGAT 
GTGAAGGCAA AATTTCCTAA AAATGCAGAA ATGAAGGGGT ATTGCCCAGT TACCTATGTT 
GATGGTAATA AAAGATACGA AGCACTGGTC CCTGGTCATA TTGAGAATGC AGTAGAATAC 
AGAGATAAGA TTTATATTTT TGAGACCAAG GAAAAGCTAC AGAAGTTCAT GAGACTTCCA 
GAGAAATACT ACAATCAGAA GCTTCCATGC AAACTTCCAC CAAAAATAGA ACCTGTGCTT 
TTGACTTCAC TTCCATTAAC TGGATACCTT GAGCAGGGAG CTGCAACTGC TTTAATAAAA 
GCAATGAATG ATGTAGGCTG CCTAAAGCCC AAGTATCCAT ATCTAAACAT CAAACAATCT 
GCTCTGCTCT ATATTGCTTT TCATTTAAAA GCTTTTAATC CTCGGAATTC TGATTACATA 
CGAAATAAAT ACAAGAAAAA GCTGGAACAA TTTGTGGAGC GATGTGAACT TATAACATAC 
CTTGGAGACA CAATGACTCG CAAGTACAAA GAACCCCAAA GAAGGCCCAT TGATTTTGAC 
TTTAAGATGC AGTCTTTCTT GTCATTGAAA GGCATGGACC CAACCTGCAT TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

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ak9 cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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