limch1 gene cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

mRNA	Protein	Name
XM_012955699.3	XP_012811153.2	LIM and calponin homology domains-containing protein 1 isoform X7
XM_004911220.4	XP_004911277.2	LIM and calponin homology domains-containing protein 1 isoform X5
XM_004911217.4	XP_004911274.2	LIM and calponin homology domains-containing protein 1 isoform X1
XM_004911217.3	XP_004911274.1	LIM and calponin homology domains-containing protein 1 isoform X1
XM_004911218.3	XP_004911275.1	LIM and calponin homology domains-containing protein 1 isoform X2
XM_012955695.2	XP_012811149.1	LIM and calponin homology domains-containing protein 1 isoform X3
XM_012955696.3	XP_012811150.2	LIM and calponin homology domains-containing protein 1 isoform X3
XM_012955698.2	XP_012811152.1	LIM and calponin homology domains-containing protein 1 isoform X5
XM_004911220.3	XP_004911277.1	LIM and calponin homology domains-containing protein 1 isoform X6
XM_004911221.3	XP_004911278.1	LIM and calponin homology domains-containing protein 1 isoform X6
XM_012955696.2	XP_012811150.1	LIM and calponin homology domains-containing protein 1 isoform X4
XM_031895385.1	XP_031751245.1	LIM and calponin homology domains-containing protein 1 isoform X6
XM_031895386.1	XP_031751246.1	LIM and calponin homology domains-containing protein 1 isoform X8
XM_012955698.3	XP_012811152.2	LIM and calponin homology domains-containing protein 1 isoform X4
XM_012955699.2	XP_012811153.1	LIM and calponin homology domains-containing protein 1 isoform X7
XM_004911218.4	XP_004911275.2	LIM and calponin homology domains-containing protein 1 isoform X2
XM_004911221.4	XP_004911278.2	LIM and calponin homology domains-containing protein 1 isoform X5

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OXa04746	XM_004911217.3 Latest version!	Xenopus tropicalis LIM and calponin homology domains 1 (limch1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OXa04747	XM_004911218.3 Latest version!	Xenopus tropicalis LIM and calponin homology domains 1 (limch1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OXa04749	XM_004911221.3 Latest version!	Xenopus tropicalis LIM and calponin homology domains 1 (limch1), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OXa04749	XM_004911220.3 Latest version!	Xenopus tropicalis LIM and calponin homology domains 1 (limch1), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OXa30867	XM_012955695.2 Latest version!	Xenopus tropicalis LIM and calponin homology domains 1 (limch1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OXa30868	XM_012955696.2 Latest version!	Xenopus tropicalis LIM and calponin homology domains 1 (limch1), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OXa30869	XM_012955698.2 Latest version!	Xenopus tropicalis LIM and calponin homology domains 1 (limch1), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OXa30870	XM_012955699.2 Latest version!	Xenopus tropicalis LIM and calponin homology domains 1 (limch1), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OXa49695	XM_004911217.4 Latest version!	Xenopus tropicalis LIM and calponin homology domains 1 (limch1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OXa49696	XM_004911218.4 Latest version!	Xenopus tropicalis LIM and calponin homology domains 1 (limch1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OXa49697	XM_012955696.3 Latest version!	Xenopus tropicalis LIM and calponin homology domains 1 (limch1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OXa49698	XM_012955698.3 Latest version!	Xenopus tropicalis LIM and calponin homology domains 1 (limch1), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OXa49699	XM_004911221.4 Latest version!	Xenopus tropicalis LIM and calponin homology domains 1 (limch1), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OXa49699	XM_004911220.4 Latest version!	Xenopus tropicalis LIM and calponin homology domains 1 (limch1), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OXa49700	XM_031895385.1 Latest version!	Xenopus tropicalis LIM and calponin homology domains 1 (limch1), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OXa49701	XM_012955699.3 Latest version!	Xenopus tropicalis LIM and calponin homology domains 1 (limch1), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OXa49702	XM_031895386.1 Latest version!	Xenopus tropicalis LIM and calponin homology domains 1 (limch1), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00

limch1 ( XM_004911217.3 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_004911274.1 Xenopus tropicalis LIM and calponin homology domains 1 (limch1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OXa04746
Clone ID Related Accession (Same CDS sequence)	XM_004911217.3
Accession Version	XM_004911217.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5775bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1473177600000
Organism	Xenopus tropicalis(tropical clawed frog)
Product	LIM and calponin homology domains-containing protein 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_004911217.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCAGCT CCGCCACACT GCAAGGGGAA GAGATCCCCC CGCCCCAGGA GTCTCCCCCA 
GACACGGCTT GCAGGGAGGC ACAGAAGTGG ATTGAGCAAG TTACTGGCAA AAGCTTTGGC 
GACAAAGACT TCAGGACTGG ACTGGAGAAT GGAATCCTTC TGTGCGAGTT ATTAAATGCT 
ATTAAACCAG GACTCGTAAA AAAGATCAAC AGACTTCCTA CTCCCATTGC TGGATTGGAC 
AATATAACAT TATTCCTTAG AGGCTGCAAA GAGCTCGGGC TGAAAGAATC TCAGTTGTTT 
GACCCAGGTG ACCTACAGGA CACAGCAAAC AGGACAACAG CCAGGAATTC AGATTGCAAC 
AGAAAACTAA GAAATGTACT AATCACCGTG TACTGGCTGG GGAAAGCTGC AAGCAGCTGC 
TCGTCCTATA GTGGCAACAA CCTGGACTTA AAGGAGTTTG AGGGATTGCT GGCACAAATG 
AGAAAAGAAA ATGAGGAAAT AGAGAGTCCA AAACGCAGCA TACGGGACAG TGGCTACATA 
GATTGTTGGG ACTCTGAGAG AAGTGACTCC CTTTCTCCTC CCAGACATGG TAGAGATGAC 
TCCTTCGATA GTTTAGACTC ATTTGGATCT AGATCACAAC AGACACCATC TCCAGATGTA 
GTGCTGAGGG GAAGCAGCGA CGGCAGAGGA AGCGACTCTG AGTCTGACCT ACCTCACAGA 
AGGTTACCTG ATGTGCGAAA AGATGATATG TCTGCTAGAC GGGTATCCTA CGGTGAAAAC 
AAAGCATTTG TGCCTTTCAA CCAATACCTC CCCAACAAGA GCAACCAGAC CTCCTACATC 
CCGGCACCCC TGAGGAAGAA GAAGGCTGAG CGTGAGGACT ACCGGAAAAG CTGGAGCACA 
GCTACATCCC CCCTGGGGGG AGAGCGACCC TTCAGTCCCA AACAAAGTCC AGTCATTAAA 
GAAGAACAAA ACGAGTTGCA GTGTGAAGGA GAAGGGACCA AGCAGATGAT TGCCTTAAAA 
GAACCTTGCC TTGGAGCAGA AAATAGGAGT GAGAGAGTTG GTGAAAGTGA AGGGGGTGTC 
ATGGGACAAT CATCTGCTAA TGATGACATT CCAAAAGATG CAGAGGAAGT GAGAAAGCGC 
AAGCACCTTC AGAAAGCGGG TATCAAGGTC TTGCCCGCAA CAAAGCGCTA CAGCAGTCCA 
AGTCAATTTG CTGAAGACAT TGATGGCGCT CCAGACATTG TGCTGCGAAA AGAGAACACC 
TTCCTGAAGT CATATGAAGG GATGGGCTCT GATTCCGAAG AAGAAGAAGA GTGCAAAGTA 
CCTGATATAC AGCGAGATGA CTTGGCCACA AGGCGTGCAA GGATGAACCA ACCAAAAAGC 
CCAATGCAGT TTAATCAGTA TATACAGGGG TCATACTTAA ACAAAAACAG AGCATCAAGA 
AAGCTGAACA GTTCTAAGAC TTCCACTCCA GCTTCTCCAA AAATGTCTCA AGATATCAGG 
GAGTACCAAG GCCCCCAGTT ATTAAAGCCA TCACACGTCT GCAAAGAAAA GACATTTCCA 
ATACCCTCAG ACAATAAAGA GGATGCAGAA GCAGAGCAAG AAAAAGTTCC CGACATTCAG 
AAAGACGACC TGGCGAAACG CAAAGCTAAA GCTTCAGTTT CACTGCCAAA GGAACCATCG 
GCCTTTGCAG CGGTTTCAAT TACAAAGGCC GACATGGAAA CTTGGCAGAG GCTTCGAATG 
TCTTCTGAAG ATAGTGAGCA CTTGAATGAC AGGCAGGCTG ACCAGCAAGA GCAAACATCC 
AAGATTGATG TGACTAGAGC GACAAATGAT GACTTTGGGA GGAGGAAATT GTCTCTGCAA 
GAGAAAAGGG CTCGAAACAA ACAGAAGTTT GTACGCTTTG GCCCAGTGAC TGAAATTGAT 
CAGCAGAAGT GGGATAGGCT TTGTATAGCC AAGGCTGAAT CTGGCCAGGA AAGTGAAGAG 
ATGAAGGGCA GCATTACGTC TGATGAAACT GCCAGCCACA TATCTCCCTC AGCTTCTGCT 
CAGCGCTCCC AACCCCAGGA GGATGTTGGC ACAGGGCCAG TCTTGCCACA TGAGTCAAGA 
TCTTTTACAC CAGAGAGATT GGTTTCACCT GCTCCTCCTG AAAATGAAAG TGAAATTCAG 
CTCCTAGAAA CACAGCAAGA TGACATGATG GCAAGGAGAA CAGGTACATT CCAAAAATGC 
ACCAGAACCA GCAGAGACTC TCCTGTGTCC CACCAATATG CACTCCATAG CCAACAACAC 
GAAGAGAAGT CTGACTCGCT GGAACTCGAA GAGAAACAAG GACTCTTTCT GGATGTTAAC 
CCACAACTGA GAAAATTACT GCTGTCGGAC TTGGCAAATA CTAGTTCAAA AGCAACTAGC 
CCTACTTTGG GGAGCAAGTC TCTGAAAGTC ACCAAAGAAG CTGAAGATGT AAAAAATGAC 
ATGAAAGAAG GTGCCTTGAA CACAGGAAAG GATCCAAACT CTGTTATCCC AGAGGATAAC 
ACAATTGCAC CAGACCCAGA AAAAGATGAC ATGATGACAA GAAAAATCTC AGTGAGCCAG 
AAGCCATCTT GCGTCAGCCT TGGACACTTC CTTCCTGTTC CTTTTTCTTT GCAGCACAGA 
GAGGGGGAAA AGAAACCAAC AAGCTGCATC ATATCAAGCA CCAAAAAGTC CAAAAATGCA 
TCCAAAGCTG TTCCAGCCGA CATTTCTGAT GTTCCTTCTA AGCCCCTTGA CCAGAAAGAA 
ACAAAAGAAC ATGGTGAATT AGGAGATACG ACTTGCAAGC AAGCCTCCAA TAGTGTGGAG 
ACTCCCATGT GTACAGAGTT AGACAGCCAG CAACAGGATG GTCTTGAGAG GAGAATGATA 
CTGTCTACTG ACTTGGCCAA TACTGAGTTA AAAATAACTC CTCCTACTTT GGCCAACAAC 
TCTCCAACCA TAACAAAAGT ACAAGAGCCA GATGCAACCA TCTCAGAGGA CAATACAACT 
GCACCAAACC CTGAAAAAGA TGATATGATG ACCAGAAGAA TCTATGTAAC CCAGAAACCA 
TCGTGTGTAG GTCTTGGCCA CTTCCTTCCA GTTCCATTTT CTTTGCAGCA AAAAGTTGAG 
GAAAAGAAAT CAACGAGCTC CACTCTATCG ATCAAAATAA AGTCAGATAA TTGCACCCAA 
GCACTTGCAG TCGGCAGTTC TGACCTTCCT TGTAAGCTCC TCGGCCAATG TGAAACAGAG 
GGACATGTTG AATTACGATC CGATGTGACT TGCGATCAAA CCTCCACTGA GATCATTAGT 
GATGGGATTC CCACTGGTAA AGGGTTAGAT ACCCAGGCAG AAGACGCTCT TGAGACATGT 
GACCATGAGT TATTCAGATT CAGCCCAAGA TCTGTTCTGT CTGATGATGG AGAGAGCATG 
AGCATGTGCG ACATGAGGTA CGAAGAAGAG GGGAGTATGC AGCCTCACAG CAGGGCACAC 
TATGAGCATC TACAGCATGT CAACAGCTTG CTCAAGGAGG AGGATGACAC ATGGCAAGAT 
GACCTAGCTA AGTGGAAGAC ACGGAGAAGA AGTGCATCAC AGGATCTGAT CAAAAAAGAG 
GAACAGAGGA AGAAGATGGA GAGAATGATG AGCGGAGAGG ATGAACTGTC GGAGCGTAGG 
AAAAGCATAA AAACATATAG AGAGATTGTG GAAGAAAAAG AACGTCGAGA GAAAGAACTG 
CATGAAGCCT ACAAAAATGC CAAGTCAAGA GATGAGGCTG AACACATATT ACAGAGCTAC 
ATTGAAAAGT TTACAATTAG TGAAGCTGTT CTTGAGGGAT TAGTAATGCC AAAAATCTTA 
GAAAGAAGCC AGTCGGTGGA GCCAAGTTTA TTGTCTTCAT CAGAGGACCC AAACATCCTG 
AAATACCTGA GGCAACAGTC TTTACCATCA CCAAAATACA CTTCCACTGT TGAAGCAACT 
GTCTCCCCCA GCAGCCCATG TGAAGCAAAA AGTCCAACAG GTCTGATATC ACCAAGCAAA 
AACATTATCT CAAAGACTGT GCCTATGCTA ACAGCCAAGC CTTACTCCCA GCCCAAAAAC 
ACCCAGGAAG TCCTCAAGAC ATTTAAGGTA GATGGAAAGG TAAACCTCAA CGGGGTAGAA 
GAGCAGAAAG AAGAGGAACA TGGAAACCAG ACATTTGCCC CATCACTCAC CCGATCTCAG 
ATGTTTGAAG GTGTAGCCAG AGTTGATGTT CCTGTTGTTG AAATAAAGCA AGACGTAACT 
TCCATAGAGC TTAATCTGGC CAGACCAAAC ACCCTGAATT CCTTACAAGA ACCCAAAAGT 
CTCTGCGAAG ACAGAAATCA CATAAGCCAG GAGGATGAGT CAGATTCTCT GGTGCAATTA 
GAAAGAGCTC ACTCCGTGGA AATATTGTCA GAAGGTAACA CCCAATCAAG CAATGCAACC 
CAGGATGTAC CTGTTGTGAG CAGTATAGAG ATTCCACCAT TGACCATCAA TGGAAAGGAC 
ACTGTGGATG AGAGGAAAGT GGAAGCTGCC GAGTTGATCA TGCATTGGTC CCTGGAGCCG 
AATCATAAGG AAGCCAAAGA AAATTCTTTA TCGCCTTTAA AAACTTCTGA AAATTTAGAA 
GAATCCTTGC AGTTTAAAGA GAATGAATCA TCACACAAAG TGCAGCTAAA CATTACGCTT 
CAGAGCCGGA ACGCCTGGAG ACGCTCCCAG TTTTTTTTGC AGCCAGTGGA TACAGAATGT 
ACAGAAAATT CACCAGCACT TGTACCCAGT TTGAATTCTC CTAACCGATT CTGCACATGG 
GACCCTGAAG AAGAACGCAA ACGGCAAGAA AAATGGCAGC AGGAGCAGGA AAGGCTTCTC 
CAGGAGAGGT ACCAGAGAGA GCAGGAAAAA CTTAAAGAAG AATGGGAAAA AGCTCAGAAA 
GAAGTAGAAG AGGAGGAAAG GAAATATTAT GAGGAGGAAA GAAAGATAAT TGAAGACACT 
GTGGTCCCAC TGACTTTGTC TCCAAATTCT AATGCACAGT TTAATATCTC TGAAAGAAAT 
GGGAACTTCA TTCCAGCACT TACAGAACAT GTCAAGAGAG ATCAAGAAAA TAACAATGAA 
AAGCTCTTGA CAGAGTCCAG CAGAACGATA AAGAATGAGA CACATATGCA CAGCAATGTA 
ACACAGCAAC TGCTTTTGCA TACACAGGAG GGGTCAATTC TTGGTGCTAA AGAGAAACAA 
ATAAAGGGAA AAACAGACAA GTGGGAAGCA GAACAGTCCA TCTGTATTAT GGACCGAGAG 
GAGCTGAGCA ACCATGTGCA GTCTAATGAA TGTAATACAC CCACTAGGTT ACCCTTGCAG 
GCAGGATGTA AACAAGTGAC GTCATCAGAG TCAAGTCTGC CTGGGGATCT TGGATCACCA 
AAAAATCCTA TCACTGAAAA AGATGCTATT CAGAATATGT CGTCCATAAT ACCCACACGG 
GGAACTGATT TCAAGGAAGA TACAATGAAT TCTTCACAGT CCTCTACATC ACAGTGCCAG 
TCACCAAACA GGTCTGTTAG TGGGAAAAAA CTGTGCTCCA CATGTGGACT TCCTCTAGGC 
AAGGGAGCTG CCATGATAAT TGAAACCCTC AGTCTTTATT TCCACATCCA GTGCTTCAAG 
TGTGGACTGT GTAAGGGACA ACTTGGGGAT GCGATCACCG GCACAGACGT CAGAATTCGA 
AATGGCCTAC TGAACTGCAA TGACTGTTAC ATGAAGTCTA GAAGTAAGGC GGCTGGCCAC 
CCCACCCCTT TGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004911274.1
CDS	492..6266
Translation	MASSATLQGEEIPPPQESPPDTACREAQKWIEQVTGKSFGDKDFRTGLENGILLCELLNAIKPGLVKKINRLPTPIAGLDNITLFLRGCKELGLKESQLFDPGDLQDTANRTTARNSDCNRKLRNVLITVYWLGKAASSCSSYSGNNLDLKEFEGLLAQMRKENEEIESPKRSIRDSGYIDCWDSERSDSLSPPRHGRDDSFDSLDSFGSRSQQTPSPDVVLRGSSDGRGSDSESDLPHRRLPDVRKDDMSARRVSYGENKAFVPFNQYLPNKSNQTSYIPAPLRKKKAEREDYRKSWSTATSPLGGERPFSPKQSPVIKEEQNELQCEGEGTKQMIALKEPCLGAENRSERVGESEGGVMGQSSANDDIPKDAEEVRKRKHLQKAGIKVLPATKRYSSPSQFAEDIDGAPDIVLRKENTFLKSYEGMGSDSEEEEECKVPDIQRDDLATRRARMNQPKSPMQFNQYIQGSYLNKNRASRKLNSSKTSTPASPKMSQDIREYQGPQLLKPSHVCKEKTFPIPSDNKEDAEAEQEKVPDIQKDDLAKRKAKASVSLPKEPSAFAAVSITKADMETWQRLRMSSEDSEHLNDRQADQQEQTSKIDVTRATNDDFGRRKLSLQEKRARNKQKFVRFGPVTEIDQQKWDRLCIAKAESGQESEEMKGSITSDETASHISPSASAQRSQPQEDVGTGPVLPHESRSFTPERLVSPAPPENESEIQLLETQQDDMMARRTGTFQKCTRTSRDSPVSHQYALHSQQHEEKSDSLELEEKQGLFLDVNPQLRKLLLSDLANTSSKATSPTLGSKSLKVTKEAEDVKNDMKEGALNTGKDPNSVIPEDNTIAPDPEKDDMMTRKISVSQKPSCVSLGHFLPVPFSLQHREGEKKPTSCIISSTKKSKNASKAVPADISDVPSKPLDQKETKEHGELGDTTCKQASNSVETPMCTELDSQQQDGLERRMILSTDLANTELKITPPTLANNSPTITKVQEPDATISEDNTTAPNPEKDDMMTRRIYVTQKPSCVGLGHFLPVPFSLQQKVEEKKSTSSTLSIKIKSDNCTQALAVGSSDLPCKLLGQCETEGHVELRSDVTCDQTSTEIISDGIPTGKGLDTQAEDALETCDHELFRFSPRSVLSDDGESMSMCDMRYEEEGSMQPHSRAHYEHLQHVNSLLKEEDDTWQDDLAKWKTRRRSASQDLIKKEEQRKKMERMMSGEDELSERRKSIKTYREIVEEKERREKELHEAYKNAKSRDEAEHILQSYIEKFTISEAVLEGLVMPKILERSQSVEPSLLSSSEDPNILKYLRQQSLPSPKYTSTVEATVSPSSPCEAKSPTGLISPSKNIISKTVPMLTAKPYSQPKNTQEVLKTFKVDGKVNLNGVEEQKEEEHGNQTFAPSLTRSQMFEGVARVDVPVVEIKQDVTSIELNLARPNTLNSLQEPKSLCEDRNHISQEDESDSLVQLERAHSVEILSEGNTQSSNATQDVPVVSSIEIPPLTINGKDTVDERKVEAAELIMHWSLEPNHKEAKENSLSPLKTSENLEESLQFKENESSHKVQLNITLQSRNAWRRSQFFLQPVDTECTENSPALVPSLNSPNRFCTWDPEEERKRQEKWQQEQERLLQERYQREQEKLKEEWEKAQKEVEEEERKYYEEERKIIEDTVVPLTLSPNSNAQFNISERNGNFIPALTEHVKRDQENNNEKLLTESSRTIKNETHMHSNVTQQLLLHTQEGSILGAKEKQIKGKTDKWEAEQSICIMDREELSNHVQSNECNTPTRLPLQAGCKQVTSSESSLPGDLGSPKNPITEKDAIQNMSSIIPTRGTDFKEDTMNSSQSSTSQCQSPNRSVSGKKLCSTCGLPLGKGAAMIIETLSLYFHIQCFKCGLCKGQLGDAITGTDVRIRNGLLNCNDCYMKSRSKAAGHPTPL

Target ORF information:

RefSeq Version	XM_004911217.3
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis LIM and calponin homology domains 1 (limch1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004911217.3

ORF Insert Sequence:

ATGGCCAGCT CCGCCACACT GCAAGGGGAA GAGATCCCCC CGCCCCAGGA GTCTCCCCCA 
GACACGGCTT GCAGGGAGGC ACAGAAGTGG ATTGAGCAAG TTACTGGCAA AAGCTTTGGC 
GACAAAGACT TCAGGACTGG ACTGGAGAAT GGAATCCTTC TGTGCGAGTT ATTAAATGCT 
ATTAAACCAG GACTCGTAAA AAAGATCAAC AGACTTCCTA CTCCCATTGC TGGATTGGAC 
AATATAACAT TATTCCTTAG AGGCTGCAAA GAGCTCGGGC TGAAAGAATC TCAGTTGTTT 
GACCCAGGTG ACCTACAGGA CACAGCAAAC AGGACAACAG CCAGGAATTC AGATTGCAAC 
AGAAAACTAA GAAATGTACT AATCACCGTG TACTGGCTGG GGAAAGCTGC AAGCAGCTGC 
TCGTCCTATA GTGGCAACAA CCTGGACTTA AAGGAGTTTG AGGGATTGCT GGCACAAATG 
AGAAAAGAAA ATGAGGAAAT AGAGAGTCCA AAACGCAGCA TACGGGACAG TGGCTACATA 
GATTGTTGGG ACTCTGAGAG AAGTGACTCC CTTTCTCCTC CCAGACATGG TAGAGATGAC 
TCCTTCGATA GTTTAGACTC ATTTGGATCT AGATCACAAC AGACACCATC TCCAGATGTA 
GTGCTGAGGG GAAGCAGCGA CGGCAGAGGA AGCGACTCTG AGTCTGACCT ACCTCACAGA 
AGGTTACCTG ATGTGCGAAA AGATGATATG TCTGCTAGAC GGGTATCCTA CGGTGAAAAC 
AAAGCATTTG TGCCTTTCAA CCAATACCTC CCCAACAAGA GCAACCAGAC CTCCTACATC 
CCGGCACCCC TGAGGAAGAA GAAGGCTGAG CGTGAGGACT ACCGGAAAAG CTGGAGCACA 
GCTACATCCC CCCTGGGGGG AGAGCGACCC TTCAGTCCCA AACAAAGTCC AGTCATTAAA 
GAAGAACAAA ACGAGTTGCA GTGTGAAGGA GAAGGGACCA AGCAGATGAT TGCCTTAAAA 
GAACCTTGCC TTGGAGCAGA AAATAGGAGT GAGAGAGTTG GTGAAAGTGA AGGGGGTGTC 
ATGGGACAAT CATCTGCTAA TGATGACATT CCAAAAGATG CAGAGGAAGT GAGAAAGCGC 
AAGCACCTTC AGAAAGCGGG TATCAAGGTC TTGCCCGCAA CAAAGCGCTA CAGCAGTCCA 
AGTCAATTTG CTGAAGACAT TGATGGCGCT CCAGACATTG TGCTGCGAAA AGAGAACACC 
TTCCTGAAGT CATATGAAGG GATGGGCTCT GATTCCGAAG AAGAAGAAGA GTGCAAAGTA 
CCTGATATAC AGCGAGATGA CTTGGCCACA AGGCGTGCAA GGATGAACCA ACCAAAAAGC 
CCAATGCAGT TTAATCAGTA TATACAGGGG TCATACTTAA ACAAAAACAG AGCATCAAGA 
AAGCTGAACA GTTCTAAGAC TTCCACTCCA GCTTCTCCAA AAATGTCTCA AGATATCAGG 
GAGTACCAAG GCCCCCAGTT ATTAAAGCCA TCACACGTCT GCAAAGAAAA GACATTTCCA 
ATACCCTCAG ACAATAAAGA GGATGCAGAA GCAGAGCAAG AAAAAGTTCC CGACATTCAG 
AAAGACGACC TGGCGAAACG CAAAGCTAAA GCTTCAGTTT CACTGCCAAA GGAACCATCG 
GCCTTTGCAG CGGTTTCAAT TACAAAGGCC GACATGGAAA CTTGGCAGAG GCTTCGAATG 
TCTTCTGAAG ATAGTGAGCA CTTGAATGAC AGGCAGGCTG ACCAGCAAGA GCAAACATCC 
AAGATTGATG TGACTAGAGC GACAAATGAT GACTTTGGGA GGAGGAAATT GTCTCTGCAA 
GAGAAAAGGG CTCGAAACAA ACAGAAGTTT GTACGCTTTG GCCCAGTGAC TGAAATTGAT 
CAGCAGAAGT GGGATAGGCT TTGTATAGCC AAGGCTGAAT CTGGCCAGGA AAGTGAAGAG 
ATGAAGGGCA GCATTACGTC TGATGAAACT GCCAGCCACA TATCTCCCTC AGCTTCTGCT 
CAGCGCTCCC AACCCCAGGA GGATGTTGGC ACAGGGCCAG TCTTGCCACA TGAGTCAAGA 
TCTTTTACAC CAGAGAGATT GGTTTCACCT GCTCCTCCTG AAAATGAAAG TGAAATTCAG 
CTCCTAGAAA CACAGCAAGA TGACATGATG GCAAGGAGAA CAGGTACATT CCAAAAATGC 
ACCAGAACCA GCAGAGACTC TCCTGTGTCC CACCAATATG CACTCCATAG CCAACAACAC 
GAAGAGAAGT CTGACTCGCT GGAACTCGAA GAGAAACAAG GACTCTTTCT GGATGTTAAC 
CCACAACTGA GAAAATTACT GCTGTCGGAC TTGGCAAATA CTAGTTCAAA AGCAACTAGC 
CCTACTTTGG GGAGCAAGTC TCTGAAAGTC ACCAAAGAAG CTGAAGATGT AAAAAATGAC 
ATGAAAGAAG GTGCCTTGAA CACAGGAAAG GATCCAAACT CTGTTATCCC AGAGGATAAC 
ACAATTGCAC CAGACCCAGA AAAAGATGAC ATGATGACAA GAAAAATCTC AGTGAGCCAG 
AAGCCATCTT GCGTCAGCCT TGGACACTTC CTTCCTGTTC CTTTTTCTTT GCAGCACAGA 
GAGGGGGAAA AGAAACCAAC AAGCTGCATC ATATCAAGCA CCAAAAAGTC CAAAAATGCA 
TCCAAAGCTG TTCCAGCCGA CATTTCTGAT GTTCCTTCTA AGCCCCTTGA CCAGAAAGAA 
ACAAAAGAAC ATGGTGAATT AGGAGATACG ACTTGCAAGC AAGCCTCCAA TAGTGTGGAG 
ACTCCCATGT GTACAGAGTT AGACAGCCAG CAACAGGATG GTCTTGAGAG GAGAATGATA 
CTGTCTACTG ACTTGGCCAA TACTGAGTTA AAAATAACTC CTCCTACTTT GGCCAACAAC 
TCTCCAACCA TAACAAAAGT ACAAGAGCCA GATGCAACCA TCTCAGAGGA CAATACAACT 
GCACCAAACC CTGAAAAAGA TGATATGATG ACCAGAAGAA TCTATGTAAC CCAGAAACCA 
TCGTGTGTAG GTCTTGGCCA CTTCCTTCCA GTTCCATTTT CTTTGCAGCA AAAAGTTGAG 
GAAAAGAAAT CAACGAGCTC CACTCTATCG ATCAAAATAA AGTCAGATAA TTGCACCCAA 
GCACTTGCAG TCGGCAGTTC TGACCTTCCT TGTAAGCTCC TCGGCCAATG TGAAACAGAG 
GGACATGTTG AATTACGATC CGATGTGACT TGCGATCAAA CCTCCACTGA GATCATTAGT 
GATGGGATTC CCACTGGTAA AGGGTTAGAT ACCCAGGCAG AAGACGCTCT TGAGACATGT 
GACCATGAGT TATTCAGATT CAGCCCAAGA TCTGTTCTGT CTGATGATGG AGAGAGCATG 
AGCATGTGCG ACATGAGGTA CGAAGAAGAG GGGAGTATGC AGCCTCACAG CAGGGCACAC 
TATGAGCATC TACAGCATGT CAACAGCTTG CTCAAGGAGG AGGATGACAC ATGGCAAGAT 
GACCTAGCTA AGTGGAAGAC ACGGAGAAGA AGTGCATCAC AGGATCTGAT CAAAAAAGAG 
GAACAGAGGA AGAAGATGGA GAGAATGATG AGCGGAGAGG ATGAACTGTC GGAGCGTAGG 
AAAAGCATAA AAACATATAG AGAGATTGTG GAAGAAAAAG AACGTCGAGA GAAAGAACTG 
CATGAAGCCT ACAAAAATGC CAAGTCAAGA GATGAGGCTG AACACATATT ACAGAGCTAC 
ATTGAAAAGT TTACAATTAG TGAAGCTGTT CTTGAGGGAT TAGTAATGCC AAAAATCTTA 
GAAAGAAGCC AGTCGGTGGA GCCAAGTTTA TTGTCTTCAT CAGAGGACCC AAACATCCTG 
AAATACCTGA GGCAACAGTC TTTACCATCA CCAAAATACA CTTCCACTGT TGAAGCAACT 
GTCTCCCCCA GCAGCCCATG TGAAGCAAAA AGTCCAACAG GTCTGATATC ACCAAGCAAA 
AACATTATCT CAAAGACTGT GCCTATGCTA ACAGCCAAGC CTTACTCCCA GCCCAAAAAC 
ACCCAGGAAG TCCTCAAGAC ATTTAAGGTA GATGGAAAGG TAAACCTCAA CGGGGTAGAA 
GAGCAGAAAG AAGAGGAACA TGGAAACCAG ACATTTGCCC CATCACTCAC CCGATCTCAG 
ATGTTTGAAG GTGTAGCCAG AGTTGATGTT CCTGTTGTTG AAATAAAGCA AGACGTAACT 
TCCATAGAGC TTAATCTGGC CAGACCAAAC ACCCTGAATT CCTTACAAGA ACCCAAAAGT 
CTCTGCGAAG ACAGAAATCA CATAAGCCAG GAGGATGAGT CAGATTCTCT GGTGCAATTA 
GAAAGAGCTC ACTCCGTGGA AATATTGTCA GAAGGTAACA CCCAATCAAG CAATGCAACC 
CAGGATGTAC CTGTTGTGAG CAGTATAGAG ATTCCACCAT TGACCATCAA TGGAAAGGAC 
ACTGTGGATG AGAGGAAAGT GGAAGCTGCC GAGTTGATCA TGCATTGGTC CCTGGAGCCG 
AATCATAAGG AAGCCAAAGA AAATTCTTTA TCGCCTTTAA AAACTTCTGA AAATTTAGAA 
GAATCCTTGC AGTTTAAAGA GAATGAATCA TCACACAAAG TGCAGCTAAA CATTACGCTT 
CAGAGCCGGA ACGCCTGGAG ACGCTCCCAG TTTTTTTTGC AGCCAGTGGA TACAGAATGT 
ACAGAAAATT CACCAGCACT TGTACCCAGT TTGAATTCTC CTAACCGATT CTGCACATGG 
GACCCTGAAG AAGAACGCAA ACGGCAAGAA AAATGGCAGC AGGAGCAGGA AAGGCTTCTC 
CAGGAGAGGT ACCAGAGAGA GCAGGAAAAA CTTAAAGAAG AATGGGAAAA AGCTCAGAAA 
GAAGTAGAAG AGGAGGAAAG GAAATATTAT GAGGAGGAAA GAAAGATAAT TGAAGACACT 
GTGGTCCCAC TGACTTTGTC TCCAAATTCT AATGCACAGT TTAATATCTC TGAAAGAAAT 
GGGAACTTCA TTCCAGCACT TACAGAACAT GTCAAGAGAG ATCAAGAAAA TAACAATGAA 
AAGCTCTTGA CAGAGTCCAG CAGAACGATA AAGAATGAGA CACATATGCA CAGCAATGTA 
ACACAGCAAC TGCTTTTGCA TACACAGGAG GGGTCAATTC TTGGTGCTAA AGAGAAACAA 
ATAAAGGGAA AAACAGACAA GTGGGAAGCA GAACAGTCCA TCTGTATTAT GGACCGAGAG 
GAGCTGAGCA ACCATGTGCA GTCTAATGAA TGTAATACAC CCACTAGGTT ACCCTTGCAG 
GCAGGATGTA AACAAGTGAC GTCATCAGAG TCAAGTCTGC CTGGGGATCT TGGATCACCA 
AAAAATCCTA TCACTGAAAA AGATGCTATT CAGAATATGT CGTCCATAAT ACCCACACGG 
GGAACTGATT TCAAGGAAGA TACAATGAAT TCTTCACAGT CCTCTACATC ACAGTGCCAG 
TCACCAAACA GGTCTGTTAG TGGGAAAAAA CTGTGCTCCA CATGTGGACT TCCTCTAGGC 
AAGGGAGCTG CCATGATAAT TGAAACCCTC AGTCTTTATT TCCACATCCA GTGCTTCAAG 
TGTGGACTGT GTAAGGGACA ACTTGGGGAT GCGATCACCG GCACAGACGT CAGAATTCGA 
AATGGCCTAC TGAACTGCAA TGACTGTTAC ATGAAGTCTA GAAGTAAGGC GGCTGGCCAC 
CCCACCCCTT TGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

limch1 ( XM_004911218.3 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_004911275.1 Xenopus tropicalis LIM and calponin homology domains 1 (limch1), transcript variant X2, mRNA.

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CloneID	OXa04747
Clone ID Related Accession (Same CDS sequence)	XM_004911218.3
Accession Version	XM_004911218.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5769bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1473177600000
Organism	Xenopus tropicalis(tropical clawed frog)
Product	LIM and calponin homology domains-containing protein 1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_004911218.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCAGCT CCGCCACACT GCAAGGGGAA GAGATCCCCC CGCCCCAGGA GTCTCCCCCA 
GACACGGCTT GCAGGGAGGC ACAGAAGTGG ATTGAGCAAG TTACTGGCAA AAGCTTTGGC 
GACAAAGACT TCAGGACTGG ACTGGAGAAT GGAATCCTTC TGTGCGAGTT ATTAAATGCT 
ATTAAACCAG GACTCGTAAA AAAGATCAAC AGACTTCCTA CTCCCATTGC TGGATTGGAC 
AATATAACAT TATTCCTTAG AGGCTGCAAA GAGCTCGGGC TGAAAGAATC TCAGTTGTTT 
GACCCAGGTG ACCTACAGGA CACAGCAAAC AGGACAACAG CCAGGAATTC AGATTGCAAC 
AGAAAACTAA GAAATGTACT AATCACCGTG TACTGGCTGG GGAAAGCTGC AAGCAGCTGC 
TCGTCCTATA GTGGCAACAA CCTGGACTTA AAGGAGTTTG AGGGATTGCT GGCACAAATG 
AGAAAAGAAA ATGAGGAAAT AGAGAGTCCA AAACGCAGCA TACGGGACAG TGGCTACATA 
GATTGTTGGG ACTCTGAGAG AAGTGACTCC CTTTCTCCTC CCAGACATGG TAGAGATGAC 
TCCTTCGATA GTTTAGACTC ATTTGGATCT AGATCACAAC AGACACCATC TCCAGATGTA 
GTGCTGAGGG GAAGCAGCGA CGGCAGAGGA AGCGACTCTG AGTCTGACCT ACCTCACAGA 
AGGTTACCTG ATGTGCGAAA AGATGATATG TCTGCTAGAC GGGTATCCTA CGGTGAAAAC 
AAAGCATTTG TGCCTTTCAA CCAATACCTC CCCAACAAGA GCAACCAGAC CTCCTACATC 
CCGGCACCCC TGAGGAAGAA GAAGGCTGAG CGTGAGGACT ACCGGAAAAG CTGGAGCACA 
GCTACATCCC CCCTGGGGGG AGAGCGACCC TTCAGTCCCA AACAAAGTCC AGTCATTAAA 
GAAGAACAAA ACGAGTTGCA GTGTGAAGGA GAAGGGACCA AGCAGATGAT TGCCTTAAAA 
GAACCTTGCC TTGGAGCAGA AAATAGGAGT GAGAGAGTTG GTGAAAGTGA AGGGGGTGTC 
ATGGGACAAT CATCTGCTAA TGATGACATT CCAAAAGATG CAGAGGAAGT GAGAAAGCGC 
AAGCACCTTC AGAAAGCGGG TATCAAGGTC TTGCCCGCAA CAAAGCGCTA CAGCAGTCCA 
AGTCAATTTG CTGAAGACAT TGATGGCGCT CCAGACATTG TGCTGCGAAA AGAGAACACC 
TTCCTGAAGT CATATGAAGG GATGGGCTCT GATTCCGAAG AAGAAGAAGA GTGCAAAGTA 
CCTGATATAC AGCGAGATGA CTTGGCCACA AGGCGTGCAA GGATGAACCA ACCAAAAAGC 
CCAATGCAGT TTAATCAGTA TATACAGGGG TCATACTTAA ACAAAAACAG AGCATCAAGA 
AAGCTGAACA GTTCTAAGAC TTCCACTCCA GCTTCTCCAA AAATGTCTCA AGATATCAGG 
GAGTACCAAG GCCCCCAGTT ATTAAAGCCA TCACACGTCT GCAAAGAAAA GACATTTCCA 
ATACCCTCAG ACAATAAAGA GGATGCAGAA GCAGAGCAAG AAAAAGTTCC CGACATTCAG 
AAAGACGACC TGGCGAAACG CAAAGCTAAA GCTTCAGTTT CACTGCCAAA GGAACCATCG 
GCCTTTGCAG CGGTTTCAAT TACAAAGGCC GACATGGAAA CTTGGCAGAG GCTTCGAATG 
TCTTCTGAAG ATAGTGAGCA CTTGAATGAC AGGCAGGCTG ACCAGCAAGA GCAAACATCC 
AAGATTGATG TGACTAGAGC GACAAATGAT GACTTTGGGA GGAGGAAATT GTCTCTGCAA 
GAGAAAAGGG CTCGAAACAA ACAGAAGTTT GTACGCTTTG GCCCAGTGAC TGAAATTGAT 
CAGCAGAAGT GGGATAGGCT TTGTATAGCC AAGGCTGAAT CTGGCCAGGA AAGTGAAGAG 
ATGAAGGGCA GCATTACGTC TGATGAAACT GCCAGCCACA TATCTCCCTC AGCTTCTGCT 
CAGCGCTCCC AACCCCAGGA GGATGTTGGC ACAGGGCCAG TCTTGCCACA TGAGTCAAGA 
TCTTTTACAC CAGAGAGATT GGTTTCACCT GCTCCTCCTG AAAATGAAAG TGAAATTCAG 
CTCCTAGAAA CACAGCAAGA TGACATGATG GCAAGGAGAA CAGGTACATT CCAAAAATGC 
ACCAGAACCA GCAGAGACTC TCCTGTGTCC CACCAATATG CACTCCATAG CCAACAACAC 
GAAGAGAAGT CTGACTCGCT GGAACTCGAA GAGAAACAAG GACTCTTTCT GGATGTTAAC 
CCACAACTGA GAAAATTACT GCTGTCGGAC TTGGCAAATA CTAGTTCAAA AGCAACTAGC 
CCTACTTTGG GGAGCAAGTC TCTGAAAGTC ACCAAAGAAG CTGAAGATGT AAAAAATGAC 
ATGAAAGAAG GTGCCTTGAA CACAGGAAAG GATCCAAACT CTGTTATCCC AGAGGATAAC 
ACAATTGCAC CAGACCCAGA AAAAGATGAC ATGATGACAA GAAAAATCTC AGTGAGCCAG 
AAGCCATCTT GCGTCAGCCT TGGACACTTC CTTCCTGTTC CTTTTTCTTT GCAGCACAGA 
GAGGGGGAAA AGAAACCAAC AAGCTGCATC ATATCAAGCA CCAAAAAGTC CAAAAATGCA 
TCCAAAGCTG TTCCAGCCGA CATTTCTGAT GTTCCTTCTA AGCCCCTTGA CCAGAAAGAA 
ACAAAAGAAC ATGGTGAATT AGGAGATACG ACTTGCAAGC AAGCCTCCAA TAGTGTGGAG 
ACTCCCATGT GTACAGAGTT AGACAGCCAG CAACAGGATG GTCTTGAGAG GAGAATGATA 
CTGTCTACTG ACTTGGCCAA TACTGAGTTA AAAATAACTC CTCCTACTTT GGCCAACAAC 
TCTCCAACCA TAACAAAAGT ACAAGAGCCA GATGCAACCA TCTCAGAGGA CAATACAACT 
GCACCAAACC CTGAAAAAGA TGATATGATG ACCAGAAGAA TCTATGTAAC CCAGAAACCA 
TCGTGTGTAG GTCTTGGCCA CTTCCTTCCA GTTCCATTTT CTTTGCAGCA AAAAGTTGAG 
GAAAAGAAAT CAACGAGCTC CACTCTATCG ATCAAAATAA AGTCAGATAA TTGCACCCAA 
GCACTTGCAG TCGGCAGTTC TGACCTTCCT TGTAAGCTCC TCGGCCAATG TGAAACAGAG 
GGACATGTTG AATTACGATC CGATGTGACT TGCGATCAAA CCTCCACTGA GATCATTAGT 
GATGGGATTC CCACTGGTAA AGGGTTAGAT ACCCAGGCAG AAGACGCTCT TGAGACATGT 
GACCATGAGT TATTCAGATT CAGCCCAAGA TCTGTTCTGT CTGATGATGG AGAGAGCATG 
AGCATGTGCG ACATGAGGTA CGAAGAAGAG GGGAGTATGC AGCCTCACAG CAGGGCACAC 
TATGAGCATC TACAGCATGT CAACAGCTTG CTCAAGGAGG AGGATGACAC ATGGCAAGAT 
GACCTAGCTA AGTGGAAGAC ACGGAGAAGA AGTGCATCAC AGGATCTGAT CAAAAAAGAG 
GAACAGAGGA AGAAGATGGA GAGAATGATG AGCGGAGAGG ATGAACTGTC GGAGCGTAGG 
AAAAGCATAA AAACATATAG AGAGATTGTG GAAGAAAAAG AACGTCGAGA GAAAGAACTG 
CATGAAGCCT ACAAAAATGC CAAGTCAAGA GATGAGGCTG AACACATATT ACAGAGCTAC 
ATTGAAAAGT TTACAATTAG TGAAGCTGTT CTTGAGGGAT TAGTAATGCC AAAAATCTTA 
GAAAGAAGCC AGTCGGTGGA GCCAAGTTTA TTGTCTTCAT CAGAGGACCC AAACATCCTG 
AAATACCTGA GGCAACAGTC TTTACCATCA CCAAAATACA CTTCCACTGT TGAAGCAACT 
GTCTCCCCCA GCAGCCCATG TGAAGCAAAA AGTCCAACAG GTCTGATATC ACCAAGCAAA 
AACATTATCT CAAAGACTGT GCCTATGCTA ACAGCCAAGC CTTACTCCCA GCCCAAAAAC 
ACCCAGGAAG TCCTCAAGAC ATTTAAGGTA GATGGAAAGG TAAACCTCAA CGGGGTAGAA 
GAGCAGAAAG AAGAGGAACA TGGAAACCAG ACATTTGCCC CATCACTCAC CCGATCTCAG 
ATGTTTGAAG GTGTAGCCAG AGTTGATGTT CCTGTTGTTG AAATAAAGCA AGACGTAACT 
TCCATAGAGC TTAATCTGGC CAGACCAAAC ACCCTGAATT CCTTACAAGA ACCCAAAAGT 
CTCTGCGAAG ACAGAAATCA CATAAGCCAG GAGGATGAGT CAGATTCTCT GGTGCAATTA 
GAAAGAGCTC ACTCCGTGGA AATATTGTCA GAAGGTAACA CCCAATCAAG CAATGCAACC 
CAGGATGTAC CTGTTGTGAG CAGTATAGAG ATTCCACCAT TGACCATCAA TGGAAAGGAC 
ACTGTGGATG AGAGGAAAGT GGAAGCTGCC GAGTTGATCA TGCATTGGTC CCTGGAGCCG 
AATCATAAGG AAGCCAAAGA AAATTCTTTA TCGCCTTTAA AAACTTCTGA AAATTTAGAA 
GAATCCTTGC AGTTTAAAGA GAATGAATCA TCACACAAAG TGCAGCTAAA CATTACGCTT 
CAGAGCCGGA ACGCCTGGAG ACGCTCCCAG TTTTTTTTGC AGCCAGTGGA TACAGAATGT 
ACAGAAAATT CACCAGCACT TGTACCCAGT TTGAATTCTC CTAACCGATT CTGCACATGG 
GACCCTGAAG AAGAACGCAA ACGGCAAGAA AAATGGCAGC AGGAGCAGGA AAGGCTTCTC 
CAGGAGAGGT ACCAGAGAGA GCAGGAAAAA CTTAAAGAAG AATGGGAAAA AGCTCAGAAA 
GAAGTAGAAG AGGAGGAAAG GAAATATTAT GAGGAGGAAA GAAAGATAAT TGAAGACACT 
GTGGTCCCAC TGACTTTGTC TCCAAATTCT AATGCACAGT TTAATATCTC TGAAAGAAAT 
GGGAACTTCA TTCCAGCACT TACAGAACAT GTCAAGAGAG ATCAAGAAAA TAACAATGAA 
AAGCTCTTGA CAGAGTCCAG CAGAACGATA AAGAATGAGA CACATATGCA CAGCAATGTA 
ACACAGCAAC TGCTTTTGCA TACACAGGAG GGGTCAATTC TTGGTGCTAA AGAGAAACAA 
ATAAAGGGAA AAACAGACAA GTGGGAAGCA GAACAGTCCA TCTGTATTAT GGACCGAGAG 
GAGCTGAGCA ACCATGTGCA GTCTAATGAA TGTAATACAC CCACTAGGTT ACCCTTGCAG 
GCAGGATGTA AACAAGTGAC GTCATCAGAG TCAAGTCTGC CTGGGGATCT TGGATCACCA 
AAAAATCCTA TCACTGAAAA AGATGCTATT CAGAATATGT CGTCCATAAT ACCCACACGG 
GGAACTGATT TCAAGGAAGA TACAATGAAT TCTTCACAGT CCTCTACATC ACAGTGCCAG 
TCACCAAACA GGTCTGTTAG TGGGAAAAAA CTGTGCTCCA CATGTGGACT TCCTCTAGGC 
AAGGGAGCTG CCATGATAAT TGAAACCCTC AGTCTTTATT TCCACATCCA GTGCTTCAAG 
TGTGGACTGT GTAAGGGACA ACTTGGGGAT GCGATCACCG GCACAGACGT CAGAATTCGA 
AATGGCCTAC TGAACTGCAA TGACTGTTAC ATGAAGTCTA GAACGGCTGG CCACCCCACC 
CCTTTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004911275.1
CDS	492..6260
Translation	MASSATLQGEEIPPPQESPPDTACREAQKWIEQVTGKSFGDKDFRTGLENGILLCELLNAIKPGLVKKINRLPTPIAGLDNITLFLRGCKELGLKESQLFDPGDLQDTANRTTARNSDCNRKLRNVLITVYWLGKAASSCSSYSGNNLDLKEFEGLLAQMRKENEEIESPKRSIRDSGYIDCWDSERSDSLSPPRHGRDDSFDSLDSFGSRSQQTPSPDVVLRGSSDGRGSDSESDLPHRRLPDVRKDDMSARRVSYGENKAFVPFNQYLPNKSNQTSYIPAPLRKKKAEREDYRKSWSTATSPLGGERPFSPKQSPVIKEEQNELQCEGEGTKQMIALKEPCLGAENRSERVGESEGGVMGQSSANDDIPKDAEEVRKRKHLQKAGIKVLPATKRYSSPSQFAEDIDGAPDIVLRKENTFLKSYEGMGSDSEEEEECKVPDIQRDDLATRRARMNQPKSPMQFNQYIQGSYLNKNRASRKLNSSKTSTPASPKMSQDIREYQGPQLLKPSHVCKEKTFPIPSDNKEDAEAEQEKVPDIQKDDLAKRKAKASVSLPKEPSAFAAVSITKADMETWQRLRMSSEDSEHLNDRQADQQEQTSKIDVTRATNDDFGRRKLSLQEKRARNKQKFVRFGPVTEIDQQKWDRLCIAKAESGQESEEMKGSITSDETASHISPSASAQRSQPQEDVGTGPVLPHESRSFTPERLVSPAPPENESEIQLLETQQDDMMARRTGTFQKCTRTSRDSPVSHQYALHSQQHEEKSDSLELEEKQGLFLDVNPQLRKLLLSDLANTSSKATSPTLGSKSLKVTKEAEDVKNDMKEGALNTGKDPNSVIPEDNTIAPDPEKDDMMTRKISVSQKPSCVSLGHFLPVPFSLQHREGEKKPTSCIISSTKKSKNASKAVPADISDVPSKPLDQKETKEHGELGDTTCKQASNSVETPMCTELDSQQQDGLERRMILSTDLANTELKITPPTLANNSPTITKVQEPDATISEDNTTAPNPEKDDMMTRRIYVTQKPSCVGLGHFLPVPFSLQQKVEEKKSTSSTLSIKIKSDNCTQALAVGSSDLPCKLLGQCETEGHVELRSDVTCDQTSTEIISDGIPTGKGLDTQAEDALETCDHELFRFSPRSVLSDDGESMSMCDMRYEEEGSMQPHSRAHYEHLQHVNSLLKEEDDTWQDDLAKWKTRRRSASQDLIKKEEQRKKMERMMSGEDELSERRKSIKTYREIVEEKERREKELHEAYKNAKSRDEAEHILQSYIEKFTISEAVLEGLVMPKILERSQSVEPSLLSSSEDPNILKYLRQQSLPSPKYTSTVEATVSPSSPCEAKSPTGLISPSKNIISKTVPMLTAKPYSQPKNTQEVLKTFKVDGKVNLNGVEEQKEEEHGNQTFAPSLTRSQMFEGVARVDVPVVEIKQDVTSIELNLARPNTLNSLQEPKSLCEDRNHISQEDESDSLVQLERAHSVEILSEGNTQSSNATQDVPVVSSIEIPPLTINGKDTVDERKVEAAELIMHWSLEPNHKEAKENSLSPLKTSENLEESLQFKENESSHKVQLNITLQSRNAWRRSQFFLQPVDTECTENSPALVPSLNSPNRFCTWDPEEERKRQEKWQQEQERLLQERYQREQEKLKEEWEKAQKEVEEEERKYYEEERKIIEDTVVPLTLSPNSNAQFNISERNGNFIPALTEHVKRDQENNNEKLLTESSRTIKNETHMHSNVTQQLLLHTQEGSILGAKEKQIKGKTDKWEAEQSICIMDREELSNHVQSNECNTPTRLPLQAGCKQVTSSESSLPGDLGSPKNPITEKDAIQNMSSIIPTRGTDFKEDTMNSSQSSTSQCQSPNRSVSGKKLCSTCGLPLGKGAAMIIETLSLYFHIQCFKCGLCKGQLGDAITGTDVRIRNGLLNCNDCYMKSRTAGHPTPL

Target ORF information:

RefSeq Version	XM_004911218.3
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis LIM and calponin homology domains 1 (limch1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004911218.3

ORF Insert Sequence:

ATGGCCAGCT CCGCCACACT GCAAGGGGAA GAGATCCCCC CGCCCCAGGA GTCTCCCCCA 
GACACGGCTT GCAGGGAGGC ACAGAAGTGG ATTGAGCAAG TTACTGGCAA AAGCTTTGGC 
GACAAAGACT TCAGGACTGG ACTGGAGAAT GGAATCCTTC TGTGCGAGTT ATTAAATGCT 
ATTAAACCAG GACTCGTAAA AAAGATCAAC AGACTTCCTA CTCCCATTGC TGGATTGGAC 
AATATAACAT TATTCCTTAG AGGCTGCAAA GAGCTCGGGC TGAAAGAATC TCAGTTGTTT 
GACCCAGGTG ACCTACAGGA CACAGCAAAC AGGACAACAG CCAGGAATTC AGATTGCAAC 
AGAAAACTAA GAAATGTACT AATCACCGTG TACTGGCTGG GGAAAGCTGC AAGCAGCTGC 
TCGTCCTATA GTGGCAACAA CCTGGACTTA AAGGAGTTTG AGGGATTGCT GGCACAAATG 
AGAAAAGAAA ATGAGGAAAT AGAGAGTCCA AAACGCAGCA TACGGGACAG TGGCTACATA 
GATTGTTGGG ACTCTGAGAG AAGTGACTCC CTTTCTCCTC CCAGACATGG TAGAGATGAC 
TCCTTCGATA GTTTAGACTC ATTTGGATCT AGATCACAAC AGACACCATC TCCAGATGTA 
GTGCTGAGGG GAAGCAGCGA CGGCAGAGGA AGCGACTCTG AGTCTGACCT ACCTCACAGA 
AGGTTACCTG ATGTGCGAAA AGATGATATG TCTGCTAGAC GGGTATCCTA CGGTGAAAAC 
AAAGCATTTG TGCCTTTCAA CCAATACCTC CCCAACAAGA GCAACCAGAC CTCCTACATC 
CCGGCACCCC TGAGGAAGAA GAAGGCTGAG CGTGAGGACT ACCGGAAAAG CTGGAGCACA 
GCTACATCCC CCCTGGGGGG AGAGCGACCC TTCAGTCCCA AACAAAGTCC AGTCATTAAA 
GAAGAACAAA ACGAGTTGCA GTGTGAAGGA GAAGGGACCA AGCAGATGAT TGCCTTAAAA 
GAACCTTGCC TTGGAGCAGA AAATAGGAGT GAGAGAGTTG GTGAAAGTGA AGGGGGTGTC 
ATGGGACAAT CATCTGCTAA TGATGACATT CCAAAAGATG CAGAGGAAGT GAGAAAGCGC 
AAGCACCTTC AGAAAGCGGG TATCAAGGTC TTGCCCGCAA CAAAGCGCTA CAGCAGTCCA 
AGTCAATTTG CTGAAGACAT TGATGGCGCT CCAGACATTG TGCTGCGAAA AGAGAACACC 
TTCCTGAAGT CATATGAAGG GATGGGCTCT GATTCCGAAG AAGAAGAAGA GTGCAAAGTA 
CCTGATATAC AGCGAGATGA CTTGGCCACA AGGCGTGCAA GGATGAACCA ACCAAAAAGC 
CCAATGCAGT TTAATCAGTA TATACAGGGG TCATACTTAA ACAAAAACAG AGCATCAAGA 
AAGCTGAACA GTTCTAAGAC TTCCACTCCA GCTTCTCCAA AAATGTCTCA AGATATCAGG 
GAGTACCAAG GCCCCCAGTT ATTAAAGCCA TCACACGTCT GCAAAGAAAA GACATTTCCA 
ATACCCTCAG ACAATAAAGA GGATGCAGAA GCAGAGCAAG AAAAAGTTCC CGACATTCAG 
AAAGACGACC TGGCGAAACG CAAAGCTAAA GCTTCAGTTT CACTGCCAAA GGAACCATCG 
GCCTTTGCAG CGGTTTCAAT TACAAAGGCC GACATGGAAA CTTGGCAGAG GCTTCGAATG 
TCTTCTGAAG ATAGTGAGCA CTTGAATGAC AGGCAGGCTG ACCAGCAAGA GCAAACATCC 
AAGATTGATG TGACTAGAGC GACAAATGAT GACTTTGGGA GGAGGAAATT GTCTCTGCAA 
GAGAAAAGGG CTCGAAACAA ACAGAAGTTT GTACGCTTTG GCCCAGTGAC TGAAATTGAT 
CAGCAGAAGT GGGATAGGCT TTGTATAGCC AAGGCTGAAT CTGGCCAGGA AAGTGAAGAG 
ATGAAGGGCA GCATTACGTC TGATGAAACT GCCAGCCACA TATCTCCCTC AGCTTCTGCT 
CAGCGCTCCC AACCCCAGGA GGATGTTGGC ACAGGGCCAG TCTTGCCACA TGAGTCAAGA 
TCTTTTACAC CAGAGAGATT GGTTTCACCT GCTCCTCCTG AAAATGAAAG TGAAATTCAG 
CTCCTAGAAA CACAGCAAGA TGACATGATG GCAAGGAGAA CAGGTACATT CCAAAAATGC 
ACCAGAACCA GCAGAGACTC TCCTGTGTCC CACCAATATG CACTCCATAG CCAACAACAC 
GAAGAGAAGT CTGACTCGCT GGAACTCGAA GAGAAACAAG GACTCTTTCT GGATGTTAAC 
CCACAACTGA GAAAATTACT GCTGTCGGAC TTGGCAAATA CTAGTTCAAA AGCAACTAGC 
CCTACTTTGG GGAGCAAGTC TCTGAAAGTC ACCAAAGAAG CTGAAGATGT AAAAAATGAC 
ATGAAAGAAG GTGCCTTGAA CACAGGAAAG GATCCAAACT CTGTTATCCC AGAGGATAAC 
ACAATTGCAC CAGACCCAGA AAAAGATGAC ATGATGACAA GAAAAATCTC AGTGAGCCAG 
AAGCCATCTT GCGTCAGCCT TGGACACTTC CTTCCTGTTC CTTTTTCTTT GCAGCACAGA 
GAGGGGGAAA AGAAACCAAC AAGCTGCATC ATATCAAGCA CCAAAAAGTC CAAAAATGCA 
TCCAAAGCTG TTCCAGCCGA CATTTCTGAT GTTCCTTCTA AGCCCCTTGA CCAGAAAGAA 
ACAAAAGAAC ATGGTGAATT AGGAGATACG ACTTGCAAGC AAGCCTCCAA TAGTGTGGAG 
ACTCCCATGT GTACAGAGTT AGACAGCCAG CAACAGGATG GTCTTGAGAG GAGAATGATA 
CTGTCTACTG ACTTGGCCAA TACTGAGTTA AAAATAACTC CTCCTACTTT GGCCAACAAC 
TCTCCAACCA TAACAAAAGT ACAAGAGCCA GATGCAACCA TCTCAGAGGA CAATACAACT 
GCACCAAACC CTGAAAAAGA TGATATGATG ACCAGAAGAA TCTATGTAAC CCAGAAACCA 
TCGTGTGTAG GTCTTGGCCA CTTCCTTCCA GTTCCATTTT CTTTGCAGCA AAAAGTTGAG 
GAAAAGAAAT CAACGAGCTC CACTCTATCG ATCAAAATAA AGTCAGATAA TTGCACCCAA 
GCACTTGCAG TCGGCAGTTC TGACCTTCCT TGTAAGCTCC TCGGCCAATG TGAAACAGAG 
GGACATGTTG AATTACGATC CGATGTGACT TGCGATCAAA CCTCCACTGA GATCATTAGT 
GATGGGATTC CCACTGGTAA AGGGTTAGAT ACCCAGGCAG AAGACGCTCT TGAGACATGT 
GACCATGAGT TATTCAGATT CAGCCCAAGA TCTGTTCTGT CTGATGATGG AGAGAGCATG 
AGCATGTGCG ACATGAGGTA CGAAGAAGAG GGGAGTATGC AGCCTCACAG CAGGGCACAC 
TATGAGCATC TACAGCATGT CAACAGCTTG CTCAAGGAGG AGGATGACAC ATGGCAAGAT 
GACCTAGCTA AGTGGAAGAC ACGGAGAAGA AGTGCATCAC AGGATCTGAT CAAAAAAGAG 
GAACAGAGGA AGAAGATGGA GAGAATGATG AGCGGAGAGG ATGAACTGTC GGAGCGTAGG 
AAAAGCATAA AAACATATAG AGAGATTGTG GAAGAAAAAG AACGTCGAGA GAAAGAACTG 
CATGAAGCCT ACAAAAATGC CAAGTCAAGA GATGAGGCTG AACACATATT ACAGAGCTAC 
ATTGAAAAGT TTACAATTAG TGAAGCTGTT CTTGAGGGAT TAGTAATGCC AAAAATCTTA 
GAAAGAAGCC AGTCGGTGGA GCCAAGTTTA TTGTCTTCAT CAGAGGACCC AAACATCCTG 
AAATACCTGA GGCAACAGTC TTTACCATCA CCAAAATACA CTTCCACTGT TGAAGCAACT 
GTCTCCCCCA GCAGCCCATG TGAAGCAAAA AGTCCAACAG GTCTGATATC ACCAAGCAAA 
AACATTATCT CAAAGACTGT GCCTATGCTA ACAGCCAAGC CTTACTCCCA GCCCAAAAAC 
ACCCAGGAAG TCCTCAAGAC ATTTAAGGTA GATGGAAAGG TAAACCTCAA CGGGGTAGAA 
GAGCAGAAAG AAGAGGAACA TGGAAACCAG ACATTTGCCC CATCACTCAC CCGATCTCAG 
ATGTTTGAAG GTGTAGCCAG AGTTGATGTT CCTGTTGTTG AAATAAAGCA AGACGTAACT 
TCCATAGAGC TTAATCTGGC CAGACCAAAC ACCCTGAATT CCTTACAAGA ACCCAAAAGT 
CTCTGCGAAG ACAGAAATCA CATAAGCCAG GAGGATGAGT CAGATTCTCT GGTGCAATTA 
GAAAGAGCTC ACTCCGTGGA AATATTGTCA GAAGGTAACA CCCAATCAAG CAATGCAACC 
CAGGATGTAC CTGTTGTGAG CAGTATAGAG ATTCCACCAT TGACCATCAA TGGAAAGGAC 
ACTGTGGATG AGAGGAAAGT GGAAGCTGCC GAGTTGATCA TGCATTGGTC CCTGGAGCCG 
AATCATAAGG AAGCCAAAGA AAATTCTTTA TCGCCTTTAA AAACTTCTGA AAATTTAGAA 
GAATCCTTGC AGTTTAAAGA GAATGAATCA TCACACAAAG TGCAGCTAAA CATTACGCTT 
CAGAGCCGGA ACGCCTGGAG ACGCTCCCAG TTTTTTTTGC AGCCAGTGGA TACAGAATGT 
ACAGAAAATT CACCAGCACT TGTACCCAGT TTGAATTCTC CTAACCGATT CTGCACATGG 
GACCCTGAAG AAGAACGCAA ACGGCAAGAA AAATGGCAGC AGGAGCAGGA AAGGCTTCTC 
CAGGAGAGGT ACCAGAGAGA GCAGGAAAAA CTTAAAGAAG AATGGGAAAA AGCTCAGAAA 
GAAGTAGAAG AGGAGGAAAG GAAATATTAT GAGGAGGAAA GAAAGATAAT TGAAGACACT 
GTGGTCCCAC TGACTTTGTC TCCAAATTCT AATGCACAGT TTAATATCTC TGAAAGAAAT 
GGGAACTTCA TTCCAGCACT TACAGAACAT GTCAAGAGAG ATCAAGAAAA TAACAATGAA 
AAGCTCTTGA CAGAGTCCAG CAGAACGATA AAGAATGAGA CACATATGCA CAGCAATGTA 
ACACAGCAAC TGCTTTTGCA TACACAGGAG GGGTCAATTC TTGGTGCTAA AGAGAAACAA 
ATAAAGGGAA AAACAGACAA GTGGGAAGCA GAACAGTCCA TCTGTATTAT GGACCGAGAG 
GAGCTGAGCA ACCATGTGCA GTCTAATGAA TGTAATACAC CCACTAGGTT ACCCTTGCAG 
GCAGGATGTA AACAAGTGAC GTCATCAGAG TCAAGTCTGC CTGGGGATCT TGGATCACCA 
AAAAATCCTA TCACTGAAAA AGATGCTATT CAGAATATGT CGTCCATAAT ACCCACACGG 
GGAACTGATT TCAAGGAAGA TACAATGAAT TCTTCACAGT CCTCTACATC ACAGTGCCAG 
TCACCAAACA GGTCTGTTAG TGGGAAAAAA CTGTGCTCCA CATGTGGACT TCCTCTAGGC 
AAGGGAGCTG CCATGATAAT TGAAACCCTC AGTCTTTATT TCCACATCCA GTGCTTCAAG 
TGTGGACTGT GTAAGGGACA ACTTGGGGAT GCGATCACCG GCACAGACGT CAGAATTCGA 
AATGGCCTAC TGAACTGCAA TGACTGTTAC ATGAAGTCTA GAACGGCTGG CCACCCCACC 
CCTTTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

limch1 ( XM_004911220.3 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_004911277.1 Xenopus tropicalis LIM and calponin homology domains 1 (limch1), transcript variant X6, mRNA.

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CloneID	OXa04749
Clone ID Related Accession (Same CDS sequence)	XM_004911220.3 , XM_004911221.3
Accession Version	XM_004911220.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5604bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1473177600000
Organism	Xenopus tropicalis(tropical clawed frog)
Product	LIM and calponin homology domains-containing protein 1 isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_004911220.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCAGCT CCGCCACACT GCAAGGGGAA GAGATCCCCC CGCCCCAGGA GTCTCCCCCA 
GACACGGCTT GCAGGGAGGC ACAGAAGTGG ATTGAGCAAG TTACTGGCAA AAGCTTTGGC 
GACAAAGACT TCAGGACTGG ACTGGAGAAT GGAATCCTTC TGTGCGAGTT ATTAAATGCT 
ATTAAACCAG GACTCGTAAA AAAGATCAAC AGACTTCCTA CTCCCATTGC TGGATTGGAC 
AATATAACAT TATTCCTTAG AGGCTGCAAA GAGCTCGGGC TGAAAGAATC TCAGTTGTTT 
GACCCAGGTG ACCTACAGGA CACAGCAAAC AGGACAACAG CCAGGAATTC AGATTGCAAC 
AGAAAACTAA GAAATGTACT AATCACCGTG TACTGGCTGG GGAAAGCTGC AAGCAGCTGC 
TCGTCCTATA GTGGCAACAA CCTGGACTTA AAGGAGTTTG AGGGATTGCT GGCACAAATG 
AGAAAAGAAA ATGAGGAAAT AGAGAGTCCA AAACGCAGCA TACGGGACAG TGGCTACATA 
GATTGTTGGG ACTCTGAGAG AAGTGACTCC CTTTCTCCTC CCAGACATGG TAGAGATGAC 
TCCTTCGATA GTTTAGACTC ATTTGGATCT AGATCACAAC AGACACCATC TCCAGATGTA 
GTGCTGAGGG GAAGCAGCGA CGGCAGAGGA AGCGACTCTG AGTCTGACCT ACCTCACAGA 
AGGTTACCTG ATGTGCGAAA AGATGATATG TCTGCTAGAC GGGTATCCTA CGGTGAAAAC 
AAAGCATTTG TGCCTTTCAA CCAATACCTC CCCAACAAGA GCAACCAGAC CTCCTACATC 
CCGGCACCCC TGAGGAAGAA GAAGGCTGAG CGTGAGGACT ACCGGAAAAG CTGGAGCACA 
GCTACATCCC CCCTGGGGGG AGAGCGACCC TTCAGTCCCA AACAAAGTCC AGTCATTAAA 
GAAGAACAAA ACGAGTTGCA GTGTGAAGGA GAAGGGACCA AGCAGATGAT TGCCTTAAAA 
GAACCTTGCC TTGGAGCAGA AAATAGGAGT GAGAGAGTTG GTGAAAGTGA AGGGGGTGTC 
ATGGGACAAT CATCTGCTAA TGATGACATT CCAAAAGATG CAGAGGAAGT GAGAAAGCGC 
AAGCACCTTC AGAAAGCGGG TATCAAGGTC TTGCCCGCAA CAAAGCGCTA CAGCAGTCCA 
AGTCAATTTG CTGAAGACAT TGATGGCGCT CCAGACATTG TGCTGCGAAA AGAGAACACC 
TTCCTGAAGT CATATGAAGG GATGGGCTCT GATTCCGAAG AAGAAGAAGA GTGCAAAGTA 
CCTGATATAC AGCGAGATGA CTTGGCCACA AGGCGTGCAA GGATGAACCA ACCAAAAAGC 
CCAATGCAGT TTAATCAGTA TATACAGGGG TCATACTTAA ACAAAAACAG AGCATCAAGA 
AAGCTGAACA GTTCTAAGAC TTCCACTCCA GCTTCTCCAA AAATGTCTCA AGATATCAGG 
GAGTACCAAG GCCCCCAGTT ATTAAAGCCA TCACACGTCT GCAAAGAAAA GACATTTCCA 
ATACCCTCAG ACAATAAAGA GGATGCAGAA GCAGAGCAAG AAAAAGTTCC CGACATTCAG 
AAAGACGACC TGGCGAAACG CAAAGCTAAA GCTTCAGTTT CACTGCCAAA GGAACCATCG 
GCCTTTGCAG CGGTTTCAAT TACAAAGGCC GACATGGAAA CTTGGCAGAG GCTTCGAATG 
TCTTCTGAAG ATAGTGAGCA CTTGAATGAC AGGCAGGCTG ACCAGCAAGA GCAAACATCC 
AAGATTGATG TGACTAGAGC GACAAATGAT GACTTTGGGA GGAGGAAATT GTCTCTGCAA 
GAGAAAAGGG CTCGAAACAA ACAGAAGTTT GTACGCTTTG GCCCAGTGAC TGAAATTGAT 
CAGCAGAAGT GGGATAGGCT TTGTATAGCC AAGGCTGAAT CTGGCCAGGA AAGTGAAGAG 
ATGAAGGGCA GCATTACGTC TGATGAAACT GCCAGCCACA TATCTCCCTC AGCTTCTGCT 
CAGCGCTCCC AACCCCAGGA GGATGTTGGC ACAGGGCCAG TCTTGCCACA TGAGTCAAGA 
TCTTTTACAC CAGAGAGATT GGTTTCACCT GCTCCTCCTG AAAATGAAAG TGAAATTCAG 
CTCCTAGAAA CACAGCAAGA TGACATGATG GCAAGGAGAA CAGGTACATT CCAAAAATGC 
ACCAGAACCA GCAGAGACTC TCCTGTGTCC CACCAATATG CACTCCATAG CCAACAACAC 
GAAGAGAAGT CTGACTCGCT GGAACTCGAA GAGAAACAAG GACTCTTTCT GGATGTTAAC 
CCACAACTGA GAAAATTACT GCTGTCGGAC TTGGCAAATA CTAGTTCAAA AGCAACTAGC 
CCTACTTTGG GGAGCAAGTC TCTGAAAGTC ACCAAAGAAG CTGAAGATGT AAAAAATGAC 
ATGAAAGAAG GTGCCTTGAA CACAGGAAAG GATCCAAACT CTGTTATCCC AGAGGATAAC 
ACAATTGCAC CAGACCCAGA AAAAGATGAC ATGATGACAA GAAAAATCTC AGTGAGCCAG 
AAGCCATCTT GCGTCAGCCT TGGACACTTC CTTCCTGTTC CTTTTTCTTT GCAGCACAGA 
GAGGGGGAAA AGAAACCAAC AAGCTGCATC ATATCAAGCA CCAAAAAGTC CAAAAATGCA 
TCCAAAGCTG TTCCAGCCGA CATTTCTGAT GTTCCTTCTA AGCCCCTTGA CCAGAAAGAA 
ACAAAAGAAC ATGGTGAATT AGGAGATACG ACTTGCAAGC AAGCCTCCAA TAGTGTGGAG 
ACTCCCATGT GTACAGAGTT AGACAGCCAG CAACAGGATG GTCTTGAGAG GAGAATGATA 
CTGTCTACTG ACTTGGCCAA TACTGAGTTA AAAATAACTC CTCCTACTTT GGCCAACAAC 
TCTCCAACCA TAACAAAAGT ACAAGAGCCA GATGCAACCA TCTCAGAGGA CAATACAACT 
GCACCAAACC CTGAAAAAGA TGATATGATG ACCAGAAGAA TCTATGTAAC CCAGAAACCA 
TCGTGTGTAG GTCTTGGCCA CTTCCTTCCA GTTCCATTTT CTTTGCAGCA AAAAGTTGAG 
GAAAAGAAAT CAACGAGCTC CACTCTATCG ATCAAAATAA AGTCAGATAA TTGCACCCAA 
GCACTTGCAG TCGGCAGTTC TGACCTTCCT TGTAAGCTCC TCGGCCAATG TGAAACAGAG 
GGACATGTTG AATTACGATC CGATGTGACT TGCGATCAAA CCTCCACTGA GATCATTAGT 
GATGGGATTC CCACTGGTAA AGGGTTAGAT ACCCAGGCAG AAGACGCTCT TGAGACATGT 
GACCATGAGT TATTCAGATT CAGCCCAAGA TCTGTTCTGT CTGATGATGG AGAGAGCATG 
AGCATGTGCG ACATGAGGTA CGAAGAAGAG GGGAGTATGC AGCCTCACAG CAGGGCACAC 
TATGAGCATC TACAGCATGT CAACAGCTTG CTCAAGGAGG AGGATGACAC ATGGCAAGAT 
GACCTAGCTA AGTGGAAGAC ACGGAGAAGA AGTGCATCAC AGGATCTGAT CAAAAAAGAG 
GAACAGAGGA AGAAGATGGA GAGAATGATG AGCGGAGAGG ATGAACTGTC GGAGCGTAGG 
AAAAGCATAA AAACATATAG AGAGATTGTG GAAGAAAAAG AACGTCGAGA GAAAGAACTG 
CATGAAGCCT ACAAAAATGC CAAGTCAAGA GATGAGGCTG AACACATATT ACAGAGCTAC 
ATTGAAAAGT TTACAATTAG TGAAGCTGTT CTTGAGGGAT TAGTAATGCC AAAAATCTTA 
GAAAGAAGCC AGTCGGTGGA GCCAAGTTTA TTGTCTTCAT CAGAGGACCC AAACATCCTG 
AAATACCTGA GGCAACAGTC TTTACCATCA CCAAAATACA CTTCCACTGT TGAAGCAACT 
GTCTCCCCCA GCAGCCCATG TGAAGCAAAA AGTCCAACAG GTCTGATATC ACCAAGCAAA 
AACATTATCT CAAAGACTGT GCCTATGCTA ACAGCCAAGC CTTACTCCCA GCCCAAAAAC 
ACCCAGGAAG TCCTCAAGAC ATTTAAGGTA GATGGAAAGG TAAACCTCAA CGGGGTAGAA 
GAGCAGAAAG AAGAGGAACA TGGAAACCAG ACATTTGCCC CATCACTCAC CCGATCTCAG 
ATGTTTGAAG GTGTAGCCAG AGTTGATGTT CCTGTTGTTG AAATAAAGCA AGACGTAACT 
TCCATAGAGC TTAATCTGGC CAGACCAAAC ACCCTGAATT CCTTACAAGA ACCCAAAAGT 
CTCTGCGAAG ACAGAAATCA CATAAGCCAG GAGGATGAGT CAGATTCTCT GGTGCAATTA 
GAAAGAGCTC ACTCCGTGGA AATATTGTCA GAAGGTAACA CCCAATCAAG CAATGCAACC 
CAGGATGTAC CTGTTGTGAG CAGTATAGAG ATTCCACCAT TGACCATCAA TGGAAAGGAC 
ACTGTGGATG AGAGGAAAGT GGAAGCTGCC GAGTTGATCA TGCATTGGTC CCTGGAGCCG 
AATCATAAGG AAGCCAAAGA AAATTCTTTA TCGCCTTTAA AAACTTCTGA AAATTTAGAA 
GAATCCTTGC AGTTTAAAGA GAATGAATCA TCACACAAAG TGCAGCTAAA CATTACGCTT 
CAGAGCCGGA ACGCCTGGAG ACGCTCCCAG TTTTTTTTGC AGCCAGTGGA TACAGAATGT 
ACAGAAAATT CACCAGCACT TGTACCCAGT TTGAATTCTC CTAACCGATT CTGCACATGG 
GACCCTGAAG AAGAACGCAA ACGGCAAGAA AAATGGCAGC AGGAGCAGGA AAGGCTTCTC 
CAGGAGAGGT ACCAGAGAGA GCAGGAAAAA CTTAAAGAAG AATGGGAAAA AGCTCAGAAA 
GAAGTAGAAG AGGAGGAAAG GAAATATTAT GAGGAGGAAA GAAAGATAAT TGAAGACACT 
GTGGTCCCAC TGACTTTGTC TCCAAATTCT AATGCACAGT TTAATATCTC TGAAAGAAAT 
GGGAACTTCA TTCCAGCACT TACAGAACAT GTCAAGAGAG ATCAAGAAAA TAACAATGAA 
AAGCTCTTGA CAGAGTCCAG CAGAACGATA AAGAATGAGA CACATATGCA CAGCAATGTA 
ACACAGCAAC TGCTTTTGCA TACACAGGAG GGGTCAATTC TTGGTGCTAA AGAGAAACAA 
ATAAAGGGAA AAACAGACAA GTGGGAAGCA GAACAGTCCA TCTGTATTAT GGACCGAGAG 
GAGCTGAGCA ACCATGTGCA GTCTAATGAA TGTAATACAC CCACTAGGTT ACCCTTGCAG 
GCAGGATGTA AACAAGTGAC GTCATCAGAG TCAAGTCTGC CTGGGGATCT TGGATCACCA 
AAAAATCCTA TCACTGAAAA AGATGCTATT CAGAATATGT CGTCCATAAT ACCCACACGG 
GGAACTGATT TCAAGGAAGA TACAATGAAT TCTTCACAGT CCTCTACATC ACAGTGCCAG 
TCACCAAACA GTGTGGACTG TGTAAGGGAC AACTTGGGGA TGCGATCACC GGCACAGACG 
TCAGAATTCG AAATGGCCTA CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004911277.1
CDS	492..6095
Translation	MASSATLQGEEIPPPQESPPDTACREAQKWIEQVTGKSFGDKDFRTGLENGILLCELLNAIKPGLVKKINRLPTPIAGLDNITLFLRGCKELGLKESQLFDPGDLQDTANRTTARNSDCNRKLRNVLITVYWLGKAASSCSSYSGNNLDLKEFEGLLAQMRKENEEIESPKRSIRDSGYIDCWDSERSDSLSPPRHGRDDSFDSLDSFGSRSQQTPSPDVVLRGSSDGRGSDSESDLPHRRLPDVRKDDMSARRVSYGENKAFVPFNQYLPNKSNQTSYIPAPLRKKKAEREDYRKSWSTATSPLGGERPFSPKQSPVIKEEQNELQCEGEGTKQMIALKEPCLGAENRSERVGESEGGVMGQSSANDDIPKDAEEVRKRKHLQKAGIKVLPATKRYSSPSQFAEDIDGAPDIVLRKENTFLKSYEGMGSDSEEEEECKVPDIQRDDLATRRARMNQPKSPMQFNQYIQGSYLNKNRASRKLNSSKTSTPASPKMSQDIREYQGPQLLKPSHVCKEKTFPIPSDNKEDAEAEQEKVPDIQKDDLAKRKAKASVSLPKEPSAFAAVSITKADMETWQRLRMSSEDSEHLNDRQADQQEQTSKIDVTRATNDDFGRRKLSLQEKRARNKQKFVRFGPVTEIDQQKWDRLCIAKAESGQESEEMKGSITSDETASHISPSASAQRSQPQEDVGTGPVLPHESRSFTPERLVSPAPPENESEIQLLETQQDDMMARRTGTFQKCTRTSRDSPVSHQYALHSQQHEEKSDSLELEEKQGLFLDVNPQLRKLLLSDLANTSSKATSPTLGSKSLKVTKEAEDVKNDMKEGALNTGKDPNSVIPEDNTIAPDPEKDDMMTRKISVSQKPSCVSLGHFLPVPFSLQHREGEKKPTSCIISSTKKSKNASKAVPADISDVPSKPLDQKETKEHGELGDTTCKQASNSVETPMCTELDSQQQDGLERRMILSTDLANTELKITPPTLANNSPTITKVQEPDATISEDNTTAPNPEKDDMMTRRIYVTQKPSCVGLGHFLPVPFSLQQKVEEKKSTSSTLSIKIKSDNCTQALAVGSSDLPCKLLGQCETEGHVELRSDVTCDQTSTEIISDGIPTGKGLDTQAEDALETCDHELFRFSPRSVLSDDGESMSMCDMRYEEEGSMQPHSRAHYEHLQHVNSLLKEEDDTWQDDLAKWKTRRRSASQDLIKKEEQRKKMERMMSGEDELSERRKSIKTYREIVEEKERREKELHEAYKNAKSRDEAEHILQSYIEKFTISEAVLEGLVMPKILERSQSVEPSLLSSSEDPNILKYLRQQSLPSPKYTSTVEATVSPSSPCEAKSPTGLISPSKNIISKTVPMLTAKPYSQPKNTQEVLKTFKVDGKVNLNGVEEQKEEEHGNQTFAPSLTRSQMFEGVARVDVPVVEIKQDVTSIELNLARPNTLNSLQEPKSLCEDRNHISQEDESDSLVQLERAHSVEILSEGNTQSSNATQDVPVVSSIEIPPLTINGKDTVDERKVEAAELIMHWSLEPNHKEAKENSLSPLKTSENLEESLQFKENESSHKVQLNITLQSRNAWRRSQFFLQPVDTECTENSPALVPSLNSPNRFCTWDPEEERKRQEKWQQEQERLLQERYQREQEKLKEEWEKAQKEVEEEERKYYEEERKIIEDTVVPLTLSPNSNAQFNISERNGNFIPALTEHVKRDQENNNEKLLTESSRTIKNETHMHSNVTQQLLLHTQEGSILGAKEKQIKGKTDKWEAEQSICIMDREELSNHVQSNECNTPTRLPLQAGCKQVTSSESSLPGDLGSPKNPITEKDAIQNMSSIIPTRGTDFKEDTMNSSQSSTSQCQSPNSVDCVRDNLGMRSPAQTSEFEMAY

Target ORF information:

RefSeq Version	XM_004911220.3
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis LIM and calponin homology domains 1 (limch1), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004911220.3

ORF Insert Sequence:

ATGGCCAGCT CCGCCACACT GCAAGGGGAA GAGATCCCCC CGCCCCAGGA GTCTCCCCCA 
GACACGGCTT GCAGGGAGGC ACAGAAGTGG ATTGAGCAAG TTACTGGCAA AAGCTTTGGC 
GACAAAGACT TCAGGACTGG ACTGGAGAAT GGAATCCTTC TGTGCGAGTT ATTAAATGCT 
ATTAAACCAG GACTCGTAAA AAAGATCAAC AGACTTCCTA CTCCCATTGC TGGATTGGAC 
AATATAACAT TATTCCTTAG AGGCTGCAAA GAGCTCGGGC TGAAAGAATC TCAGTTGTTT 
GACCCAGGTG ACCTACAGGA CACAGCAAAC AGGACAACAG CCAGGAATTC AGATTGCAAC 
AGAAAACTAA GAAATGTACT AATCACCGTG TACTGGCTGG GGAAAGCTGC AAGCAGCTGC 
TCGTCCTATA GTGGCAACAA CCTGGACTTA AAGGAGTTTG AGGGATTGCT GGCACAAATG 
AGAAAAGAAA ATGAGGAAAT AGAGAGTCCA AAACGCAGCA TACGGGACAG TGGCTACATA 
GATTGTTGGG ACTCTGAGAG AAGTGACTCC CTTTCTCCTC CCAGACATGG TAGAGATGAC 
TCCTTCGATA GTTTAGACTC ATTTGGATCT AGATCACAAC AGACACCATC TCCAGATGTA 
GTGCTGAGGG GAAGCAGCGA CGGCAGAGGA AGCGACTCTG AGTCTGACCT ACCTCACAGA 
AGGTTACCTG ATGTGCGAAA AGATGATATG TCTGCTAGAC GGGTATCCTA CGGTGAAAAC 
AAAGCATTTG TGCCTTTCAA CCAATACCTC CCCAACAAGA GCAACCAGAC CTCCTACATC 
CCGGCACCCC TGAGGAAGAA GAAGGCTGAG CGTGAGGACT ACCGGAAAAG CTGGAGCACA 
GCTACATCCC CCCTGGGGGG AGAGCGACCC TTCAGTCCCA AACAAAGTCC AGTCATTAAA 
GAAGAACAAA ACGAGTTGCA GTGTGAAGGA GAAGGGACCA AGCAGATGAT TGCCTTAAAA 
GAACCTTGCC TTGGAGCAGA AAATAGGAGT GAGAGAGTTG GTGAAAGTGA AGGGGGTGTC 
ATGGGACAAT CATCTGCTAA TGATGACATT CCAAAAGATG CAGAGGAAGT GAGAAAGCGC 
AAGCACCTTC AGAAAGCGGG TATCAAGGTC TTGCCCGCAA CAAAGCGCTA CAGCAGTCCA 
AGTCAATTTG CTGAAGACAT TGATGGCGCT CCAGACATTG TGCTGCGAAA AGAGAACACC 
TTCCTGAAGT CATATGAAGG GATGGGCTCT GATTCCGAAG AAGAAGAAGA GTGCAAAGTA 
CCTGATATAC AGCGAGATGA CTTGGCCACA AGGCGTGCAA GGATGAACCA ACCAAAAAGC 
CCAATGCAGT TTAATCAGTA TATACAGGGG TCATACTTAA ACAAAAACAG AGCATCAAGA 
AAGCTGAACA GTTCTAAGAC TTCCACTCCA GCTTCTCCAA AAATGTCTCA AGATATCAGG 
GAGTACCAAG GCCCCCAGTT ATTAAAGCCA TCACACGTCT GCAAAGAAAA GACATTTCCA 
ATACCCTCAG ACAATAAAGA GGATGCAGAA GCAGAGCAAG AAAAAGTTCC CGACATTCAG 
AAAGACGACC TGGCGAAACG CAAAGCTAAA GCTTCAGTTT CACTGCCAAA GGAACCATCG 
GCCTTTGCAG CGGTTTCAAT TACAAAGGCC GACATGGAAA CTTGGCAGAG GCTTCGAATG 
TCTTCTGAAG ATAGTGAGCA CTTGAATGAC AGGCAGGCTG ACCAGCAAGA GCAAACATCC 
AAGATTGATG TGACTAGAGC GACAAATGAT GACTTTGGGA GGAGGAAATT GTCTCTGCAA 
GAGAAAAGGG CTCGAAACAA ACAGAAGTTT GTACGCTTTG GCCCAGTGAC TGAAATTGAT 
CAGCAGAAGT GGGATAGGCT TTGTATAGCC AAGGCTGAAT CTGGCCAGGA AAGTGAAGAG 
ATGAAGGGCA GCATTACGTC TGATGAAACT GCCAGCCACA TATCTCCCTC AGCTTCTGCT 
CAGCGCTCCC AACCCCAGGA GGATGTTGGC ACAGGGCCAG TCTTGCCACA TGAGTCAAGA 
TCTTTTACAC CAGAGAGATT GGTTTCACCT GCTCCTCCTG AAAATGAAAG TGAAATTCAG 
CTCCTAGAAA CACAGCAAGA TGACATGATG GCAAGGAGAA CAGGTACATT CCAAAAATGC 
ACCAGAACCA GCAGAGACTC TCCTGTGTCC CACCAATATG CACTCCATAG CCAACAACAC 
GAAGAGAAGT CTGACTCGCT GGAACTCGAA GAGAAACAAG GACTCTTTCT GGATGTTAAC 
CCACAACTGA GAAAATTACT GCTGTCGGAC TTGGCAAATA CTAGTTCAAA AGCAACTAGC 
CCTACTTTGG GGAGCAAGTC TCTGAAAGTC ACCAAAGAAG CTGAAGATGT AAAAAATGAC 
ATGAAAGAAG GTGCCTTGAA CACAGGAAAG GATCCAAACT CTGTTATCCC AGAGGATAAC 
ACAATTGCAC CAGACCCAGA AAAAGATGAC ATGATGACAA GAAAAATCTC AGTGAGCCAG 
AAGCCATCTT GCGTCAGCCT TGGACACTTC CTTCCTGTTC CTTTTTCTTT GCAGCACAGA 
GAGGGGGAAA AGAAACCAAC AAGCTGCATC ATATCAAGCA CCAAAAAGTC CAAAAATGCA 
TCCAAAGCTG TTCCAGCCGA CATTTCTGAT GTTCCTTCTA AGCCCCTTGA CCAGAAAGAA 
ACAAAAGAAC ATGGTGAATT AGGAGATACG ACTTGCAAGC AAGCCTCCAA TAGTGTGGAG 
ACTCCCATGT GTACAGAGTT AGACAGCCAG CAACAGGATG GTCTTGAGAG GAGAATGATA 
CTGTCTACTG ACTTGGCCAA TACTGAGTTA AAAATAACTC CTCCTACTTT GGCCAACAAC 
TCTCCAACCA TAACAAAAGT ACAAGAGCCA GATGCAACCA TCTCAGAGGA CAATACAACT 
GCACCAAACC CTGAAAAAGA TGATATGATG ACCAGAAGAA TCTATGTAAC CCAGAAACCA 
TCGTGTGTAG GTCTTGGCCA CTTCCTTCCA GTTCCATTTT CTTTGCAGCA AAAAGTTGAG 
GAAAAGAAAT CAACGAGCTC CACTCTATCG ATCAAAATAA AGTCAGATAA TTGCACCCAA 
GCACTTGCAG TCGGCAGTTC TGACCTTCCT TGTAAGCTCC TCGGCCAATG TGAAACAGAG 
GGACATGTTG AATTACGATC CGATGTGACT TGCGATCAAA CCTCCACTGA GATCATTAGT 
GATGGGATTC CCACTGGTAA AGGGTTAGAT ACCCAGGCAG AAGACGCTCT TGAGACATGT 
GACCATGAGT TATTCAGATT CAGCCCAAGA TCTGTTCTGT CTGATGATGG AGAGAGCATG 
AGCATGTGCG ACATGAGGTA CGAAGAAGAG GGGAGTATGC AGCCTCACAG CAGGGCACAC 
TATGAGCATC TACAGCATGT CAACAGCTTG CTCAAGGAGG AGGATGACAC ATGGCAAGAT 
GACCTAGCTA AGTGGAAGAC ACGGAGAAGA AGTGCATCAC AGGATCTGAT CAAAAAAGAG 
GAACAGAGGA AGAAGATGGA GAGAATGATG AGCGGAGAGG ATGAACTGTC GGAGCGTAGG 
AAAAGCATAA AAACATATAG AGAGATTGTG GAAGAAAAAG AACGTCGAGA GAAAGAACTG 
CATGAAGCCT ACAAAAATGC CAAGTCAAGA GATGAGGCTG AACACATATT ACAGAGCTAC 
ATTGAAAAGT TTACAATTAG TGAAGCTGTT CTTGAGGGAT TAGTAATGCC AAAAATCTTA 
GAAAGAAGCC AGTCGGTGGA GCCAAGTTTA TTGTCTTCAT CAGAGGACCC AAACATCCTG 
AAATACCTGA GGCAACAGTC TTTACCATCA CCAAAATACA CTTCCACTGT TGAAGCAACT 
GTCTCCCCCA GCAGCCCATG TGAAGCAAAA AGTCCAACAG GTCTGATATC ACCAAGCAAA 
AACATTATCT CAAAGACTGT GCCTATGCTA ACAGCCAAGC CTTACTCCCA GCCCAAAAAC 
ACCCAGGAAG TCCTCAAGAC ATTTAAGGTA GATGGAAAGG TAAACCTCAA CGGGGTAGAA 
GAGCAGAAAG AAGAGGAACA TGGAAACCAG ACATTTGCCC CATCACTCAC CCGATCTCAG 
ATGTTTGAAG GTGTAGCCAG AGTTGATGTT CCTGTTGTTG AAATAAAGCA AGACGTAACT 
TCCATAGAGC TTAATCTGGC CAGACCAAAC ACCCTGAATT CCTTACAAGA ACCCAAAAGT 
CTCTGCGAAG ACAGAAATCA CATAAGCCAG GAGGATGAGT CAGATTCTCT GGTGCAATTA 
GAAAGAGCTC ACTCCGTGGA AATATTGTCA GAAGGTAACA CCCAATCAAG CAATGCAACC 
CAGGATGTAC CTGTTGTGAG CAGTATAGAG ATTCCACCAT TGACCATCAA TGGAAAGGAC 
ACTGTGGATG AGAGGAAAGT GGAAGCTGCC GAGTTGATCA TGCATTGGTC CCTGGAGCCG 
AATCATAAGG AAGCCAAAGA AAATTCTTTA TCGCCTTTAA AAACTTCTGA AAATTTAGAA 
GAATCCTTGC AGTTTAAAGA GAATGAATCA TCACACAAAG TGCAGCTAAA CATTACGCTT 
CAGAGCCGGA ACGCCTGGAG ACGCTCCCAG TTTTTTTTGC AGCCAGTGGA TACAGAATGT 
ACAGAAAATT CACCAGCACT TGTACCCAGT TTGAATTCTC CTAACCGATT CTGCACATGG 
GACCCTGAAG AAGAACGCAA ACGGCAAGAA AAATGGCAGC AGGAGCAGGA AAGGCTTCTC 
CAGGAGAGGT ACCAGAGAGA GCAGGAAAAA CTTAAAGAAG AATGGGAAAA AGCTCAGAAA 
GAAGTAGAAG AGGAGGAAAG GAAATATTAT GAGGAGGAAA GAAAGATAAT TGAAGACACT 
GTGGTCCCAC TGACTTTGTC TCCAAATTCT AATGCACAGT TTAATATCTC TGAAAGAAAT 
GGGAACTTCA TTCCAGCACT TACAGAACAT GTCAAGAGAG ATCAAGAAAA TAACAATGAA 
AAGCTCTTGA CAGAGTCCAG CAGAACGATA AAGAATGAGA CACATATGCA CAGCAATGTA 
ACACAGCAAC TGCTTTTGCA TACACAGGAG GGGTCAATTC TTGGTGCTAA AGAGAAACAA 
ATAAAGGGAA AAACAGACAA GTGGGAAGCA GAACAGTCCA TCTGTATTAT GGACCGAGAG 
GAGCTGAGCA ACCATGTGCA GTCTAATGAA TGTAATACAC CCACTAGGTT ACCCTTGCAG 
GCAGGATGTA AACAAGTGAC GTCATCAGAG TCAAGTCTGC CTGGGGATCT TGGATCACCA 
AAAAATCCTA TCACTGAAAA AGATGCTATT CAGAATATGT CGTCCATAAT ACCCACACGG 
GGAACTGATT TCAAGGAAGA TACAATGAAT TCTTCACAGT CCTCTACATC ACAGTGCCAG 
TCACCAAACA GTGTGGACTG TGTAAGGGAC AACTTGGGGA TGCGATCACC GGCACAGACG 
TCAGAATTCG AAATGGCCTA CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

limch1 ( XM_004911221.3 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_004911278.1 Xenopus tropicalis LIM and calponin homology domains 1 (limch1), transcript variant X7, mRNA.

Price & Availability↑

CloneID	OXa04749
Clone ID Related Accession (Same CDS sequence)	XM_004911220.3 , XM_004911221.3
Accession Version	XM_004911221.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5604bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1473177600000
Organism	Xenopus tropicalis(tropical clawed frog)
Product	LIM and calponin homology domains-containing protein 1 isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_004911221.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCAGCT CCGCCACACT GCAAGGGGAA GAGATCCCCC CGCCCCAGGA GTCTCCCCCA 
GACACGGCTT GCAGGGAGGC ACAGAAGTGG ATTGAGCAAG TTACTGGCAA AAGCTTTGGC 
GACAAAGACT TCAGGACTGG ACTGGAGAAT GGAATCCTTC TGTGCGAGTT ATTAAATGCT 
ATTAAACCAG GACTCGTAAA AAAGATCAAC AGACTTCCTA CTCCCATTGC TGGATTGGAC 
AATATAACAT TATTCCTTAG AGGCTGCAAA GAGCTCGGGC TGAAAGAATC TCAGTTGTTT 
GACCCAGGTG ACCTACAGGA CACAGCAAAC AGGACAACAG CCAGGAATTC AGATTGCAAC 
AGAAAACTAA GAAATGTACT AATCACCGTG TACTGGCTGG GGAAAGCTGC AAGCAGCTGC 
TCGTCCTATA GTGGCAACAA CCTGGACTTA AAGGAGTTTG AGGGATTGCT GGCACAAATG 
AGAAAAGAAA ATGAGGAAAT AGAGAGTCCA AAACGCAGCA TACGGGACAG TGGCTACATA 
GATTGTTGGG ACTCTGAGAG AAGTGACTCC CTTTCTCCTC CCAGACATGG TAGAGATGAC 
TCCTTCGATA GTTTAGACTC ATTTGGATCT AGATCACAAC AGACACCATC TCCAGATGTA 
GTGCTGAGGG GAAGCAGCGA CGGCAGAGGA AGCGACTCTG AGTCTGACCT ACCTCACAGA 
AGGTTACCTG ATGTGCGAAA AGATGATATG TCTGCTAGAC GGGTATCCTA CGGTGAAAAC 
AAAGCATTTG TGCCTTTCAA CCAATACCTC CCCAACAAGA GCAACCAGAC CTCCTACATC 
CCGGCACCCC TGAGGAAGAA GAAGGCTGAG CGTGAGGACT ACCGGAAAAG CTGGAGCACA 
GCTACATCCC CCCTGGGGGG AGAGCGACCC TTCAGTCCCA AACAAAGTCC AGTCATTAAA 
GAAGAACAAA ACGAGTTGCA GTGTGAAGGA GAAGGGACCA AGCAGATGAT TGCCTTAAAA 
GAACCTTGCC TTGGAGCAGA AAATAGGAGT GAGAGAGTTG GTGAAAGTGA AGGGGGTGTC 
ATGGGACAAT CATCTGCTAA TGATGACATT CCAAAAGATG CAGAGGAAGT GAGAAAGCGC 
AAGCACCTTC AGAAAGCGGG TATCAAGGTC TTGCCCGCAA CAAAGCGCTA CAGCAGTCCA 
AGTCAATTTG CTGAAGACAT TGATGGCGCT CCAGACATTG TGCTGCGAAA AGAGAACACC 
TTCCTGAAGT CATATGAAGG GATGGGCTCT GATTCCGAAG AAGAAGAAGA GTGCAAAGTA 
CCTGATATAC AGCGAGATGA CTTGGCCACA AGGCGTGCAA GGATGAACCA ACCAAAAAGC 
CCAATGCAGT TTAATCAGTA TATACAGGGG TCATACTTAA ACAAAAACAG AGCATCAAGA 
AAGCTGAACA GTTCTAAGAC TTCCACTCCA GCTTCTCCAA AAATGTCTCA AGATATCAGG 
GAGTACCAAG GCCCCCAGTT ATTAAAGCCA TCACACGTCT GCAAAGAAAA GACATTTCCA 
ATACCCTCAG ACAATAAAGA GGATGCAGAA GCAGAGCAAG AAAAAGTTCC CGACATTCAG 
AAAGACGACC TGGCGAAACG CAAAGCTAAA GCTTCAGTTT CACTGCCAAA GGAACCATCG 
GCCTTTGCAG CGGTTTCAAT TACAAAGGCC GACATGGAAA CTTGGCAGAG GCTTCGAATG 
TCTTCTGAAG ATAGTGAGCA CTTGAATGAC AGGCAGGCTG ACCAGCAAGA GCAAACATCC 
AAGATTGATG TGACTAGAGC GACAAATGAT GACTTTGGGA GGAGGAAATT GTCTCTGCAA 
GAGAAAAGGG CTCGAAACAA ACAGAAGTTT GTACGCTTTG GCCCAGTGAC TGAAATTGAT 
CAGCAGAAGT GGGATAGGCT TTGTATAGCC AAGGCTGAAT CTGGCCAGGA AAGTGAAGAG 
ATGAAGGGCA GCATTACGTC TGATGAAACT GCCAGCCACA TATCTCCCTC AGCTTCTGCT 
CAGCGCTCCC AACCCCAGGA GGATGTTGGC ACAGGGCCAG TCTTGCCACA TGAGTCAAGA 
TCTTTTACAC CAGAGAGATT GGTTTCACCT GCTCCTCCTG AAAATGAAAG TGAAATTCAG 
CTCCTAGAAA CACAGCAAGA TGACATGATG GCAAGGAGAA CAGGTACATT CCAAAAATGC 
ACCAGAACCA GCAGAGACTC TCCTGTGTCC CACCAATATG CACTCCATAG CCAACAACAC 
GAAGAGAAGT CTGACTCGCT GGAACTCGAA GAGAAACAAG GACTCTTTCT GGATGTTAAC 
CCACAACTGA GAAAATTACT GCTGTCGGAC TTGGCAAATA CTAGTTCAAA AGCAACTAGC 
CCTACTTTGG GGAGCAAGTC TCTGAAAGTC ACCAAAGAAG CTGAAGATGT AAAAAATGAC 
ATGAAAGAAG GTGCCTTGAA CACAGGAAAG GATCCAAACT CTGTTATCCC AGAGGATAAC 
ACAATTGCAC CAGACCCAGA AAAAGATGAC ATGATGACAA GAAAAATCTC AGTGAGCCAG 
AAGCCATCTT GCGTCAGCCT TGGACACTTC CTTCCTGTTC CTTTTTCTTT GCAGCACAGA 
GAGGGGGAAA AGAAACCAAC AAGCTGCATC ATATCAAGCA CCAAAAAGTC CAAAAATGCA 
TCCAAAGCTG TTCCAGCCGA CATTTCTGAT GTTCCTTCTA AGCCCCTTGA CCAGAAAGAA 
ACAAAAGAAC ATGGTGAATT AGGAGATACG ACTTGCAAGC AAGCCTCCAA TAGTGTGGAG 
ACTCCCATGT GTACAGAGTT AGACAGCCAG CAACAGGATG GTCTTGAGAG GAGAATGATA 
CTGTCTACTG ACTTGGCCAA TACTGAGTTA AAAATAACTC CTCCTACTTT GGCCAACAAC 
TCTCCAACCA TAACAAAAGT ACAAGAGCCA GATGCAACCA TCTCAGAGGA CAATACAACT 
GCACCAAACC CTGAAAAAGA TGATATGATG ACCAGAAGAA TCTATGTAAC CCAGAAACCA 
TCGTGTGTAG GTCTTGGCCA CTTCCTTCCA GTTCCATTTT CTTTGCAGCA AAAAGTTGAG 
GAAAAGAAAT CAACGAGCTC CACTCTATCG ATCAAAATAA AGTCAGATAA TTGCACCCAA 
GCACTTGCAG TCGGCAGTTC TGACCTTCCT TGTAAGCTCC TCGGCCAATG TGAAACAGAG 
GGACATGTTG AATTACGATC CGATGTGACT TGCGATCAAA CCTCCACTGA GATCATTAGT 
GATGGGATTC CCACTGGTAA AGGGTTAGAT ACCCAGGCAG AAGACGCTCT TGAGACATGT 
GACCATGAGT TATTCAGATT CAGCCCAAGA TCTGTTCTGT CTGATGATGG AGAGAGCATG 
AGCATGTGCG ACATGAGGTA CGAAGAAGAG GGGAGTATGC AGCCTCACAG CAGGGCACAC 
TATGAGCATC TACAGCATGT CAACAGCTTG CTCAAGGAGG AGGATGACAC ATGGCAAGAT 
GACCTAGCTA AGTGGAAGAC ACGGAGAAGA AGTGCATCAC AGGATCTGAT CAAAAAAGAG 
GAACAGAGGA AGAAGATGGA GAGAATGATG AGCGGAGAGG ATGAACTGTC GGAGCGTAGG 
AAAAGCATAA AAACATATAG AGAGATTGTG GAAGAAAAAG AACGTCGAGA GAAAGAACTG 
CATGAAGCCT ACAAAAATGC CAAGTCAAGA GATGAGGCTG AACACATATT ACAGAGCTAC 
ATTGAAAAGT TTACAATTAG TGAAGCTGTT CTTGAGGGAT TAGTAATGCC AAAAATCTTA 
GAAAGAAGCC AGTCGGTGGA GCCAAGTTTA TTGTCTTCAT CAGAGGACCC AAACATCCTG 
AAATACCTGA GGCAACAGTC TTTACCATCA CCAAAATACA CTTCCACTGT TGAAGCAACT 
GTCTCCCCCA GCAGCCCATG TGAAGCAAAA AGTCCAACAG GTCTGATATC ACCAAGCAAA 
AACATTATCT CAAAGACTGT GCCTATGCTA ACAGCCAAGC CTTACTCCCA GCCCAAAAAC 
ACCCAGGAAG TCCTCAAGAC ATTTAAGGTA GATGGAAAGG TAAACCTCAA CGGGGTAGAA 
GAGCAGAAAG AAGAGGAACA TGGAAACCAG ACATTTGCCC CATCACTCAC CCGATCTCAG 
ATGTTTGAAG GTGTAGCCAG AGTTGATGTT CCTGTTGTTG AAATAAAGCA AGACGTAACT 
TCCATAGAGC TTAATCTGGC CAGACCAAAC ACCCTGAATT CCTTACAAGA ACCCAAAAGT 
CTCTGCGAAG ACAGAAATCA CATAAGCCAG GAGGATGAGT CAGATTCTCT GGTGCAATTA 
GAAAGAGCTC ACTCCGTGGA AATATTGTCA GAAGGTAACA CCCAATCAAG CAATGCAACC 
CAGGATGTAC CTGTTGTGAG CAGTATAGAG ATTCCACCAT TGACCATCAA TGGAAAGGAC 
ACTGTGGATG AGAGGAAAGT GGAAGCTGCC GAGTTGATCA TGCATTGGTC CCTGGAGCCG 
AATCATAAGG AAGCCAAAGA AAATTCTTTA TCGCCTTTAA AAACTTCTGA AAATTTAGAA 
GAATCCTTGC AGTTTAAAGA GAATGAATCA TCACACAAAG TGCAGCTAAA CATTACGCTT 
CAGAGCCGGA ACGCCTGGAG ACGCTCCCAG TTTTTTTTGC AGCCAGTGGA TACAGAATGT 
ACAGAAAATT CACCAGCACT TGTACCCAGT TTGAATTCTC CTAACCGATT CTGCACATGG 
GACCCTGAAG AAGAACGCAA ACGGCAAGAA AAATGGCAGC AGGAGCAGGA AAGGCTTCTC 
CAGGAGAGGT ACCAGAGAGA GCAGGAAAAA CTTAAAGAAG AATGGGAAAA AGCTCAGAAA 
GAAGTAGAAG AGGAGGAAAG GAAATATTAT GAGGAGGAAA GAAAGATAAT TGAAGACACT 
GTGGTCCCAC TGACTTTGTC TCCAAATTCT AATGCACAGT TTAATATCTC TGAAAGAAAT 
GGGAACTTCA TTCCAGCACT TACAGAACAT GTCAAGAGAG ATCAAGAAAA TAACAATGAA 
AAGCTCTTGA CAGAGTCCAG CAGAACGATA AAGAATGAGA CACATATGCA CAGCAATGTA 
ACACAGCAAC TGCTTTTGCA TACACAGGAG GGGTCAATTC TTGGTGCTAA AGAGAAACAA 
ATAAAGGGAA AAACAGACAA GTGGGAAGCA GAACAGTCCA TCTGTATTAT GGACCGAGAG 
GAGCTGAGCA ACCATGTGCA GTCTAATGAA TGTAATACAC CCACTAGGTT ACCCTTGCAG 
GCAGGATGTA AACAAGTGAC GTCATCAGAG TCAAGTCTGC CTGGGGATCT TGGATCACCA 
AAAAATCCTA TCACTGAAAA AGATGCTATT CAGAATATGT CGTCCATAAT ACCCACACGG 
GGAACTGATT TCAAGGAAGA TACAATGAAT TCTTCACAGT CCTCTACATC ACAGTGCCAG 
TCACCAAACA GTGTGGACTG TGTAAGGGAC AACTTGGGGA TGCGATCACC GGCACAGACG 
TCAGAATTCG AAATGGCCTA CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004911278.1
CDS	492..6095
Translation	MASSATLQGEEIPPPQESPPDTACREAQKWIEQVTGKSFGDKDFRTGLENGILLCELLNAIKPGLVKKINRLPTPIAGLDNITLFLRGCKELGLKESQLFDPGDLQDTANRTTARNSDCNRKLRNVLITVYWLGKAASSCSSYSGNNLDLKEFEGLLAQMRKENEEIESPKRSIRDSGYIDCWDSERSDSLSPPRHGRDDSFDSLDSFGSRSQQTPSPDVVLRGSSDGRGSDSESDLPHRRLPDVRKDDMSARRVSYGENKAFVPFNQYLPNKSNQTSYIPAPLRKKKAEREDYRKSWSTATSPLGGERPFSPKQSPVIKEEQNELQCEGEGTKQMIALKEPCLGAENRSERVGESEGGVMGQSSANDDIPKDAEEVRKRKHLQKAGIKVLPATKRYSSPSQFAEDIDGAPDIVLRKENTFLKSYEGMGSDSEEEEECKVPDIQRDDLATRRARMNQPKSPMQFNQYIQGSYLNKNRASRKLNSSKTSTPASPKMSQDIREYQGPQLLKPSHVCKEKTFPIPSDNKEDAEAEQEKVPDIQKDDLAKRKAKASVSLPKEPSAFAAVSITKADMETWQRLRMSSEDSEHLNDRQADQQEQTSKIDVTRATNDDFGRRKLSLQEKRARNKQKFVRFGPVTEIDQQKWDRLCIAKAESGQESEEMKGSITSDETASHISPSASAQRSQPQEDVGTGPVLPHESRSFTPERLVSPAPPENESEIQLLETQQDDMMARRTGTFQKCTRTSRDSPVSHQYALHSQQHEEKSDSLELEEKQGLFLDVNPQLRKLLLSDLANTSSKATSPTLGSKSLKVTKEAEDVKNDMKEGALNTGKDPNSVIPEDNTIAPDPEKDDMMTRKISVSQKPSCVSLGHFLPVPFSLQHREGEKKPTSCIISSTKKSKNASKAVPADISDVPSKPLDQKETKEHGELGDTTCKQASNSVETPMCTELDSQQQDGLERRMILSTDLANTELKITPPTLANNSPTITKVQEPDATISEDNTTAPNPEKDDMMTRRIYVTQKPSCVGLGHFLPVPFSLQQKVEEKKSTSSTLSIKIKSDNCTQALAVGSSDLPCKLLGQCETEGHVELRSDVTCDQTSTEIISDGIPTGKGLDTQAEDALETCDHELFRFSPRSVLSDDGESMSMCDMRYEEEGSMQPHSRAHYEHLQHVNSLLKEEDDTWQDDLAKWKTRRRSASQDLIKKEEQRKKMERMMSGEDELSERRKSIKTYREIVEEKERREKELHEAYKNAKSRDEAEHILQSYIEKFTISEAVLEGLVMPKILERSQSVEPSLLSSSEDPNILKYLRQQSLPSPKYTSTVEATVSPSSPCEAKSPTGLISPSKNIISKTVPMLTAKPYSQPKNTQEVLKTFKVDGKVNLNGVEEQKEEEHGNQTFAPSLTRSQMFEGVARVDVPVVEIKQDVTSIELNLARPNTLNSLQEPKSLCEDRNHISQEDESDSLVQLERAHSVEILSEGNTQSSNATQDVPVVSSIEIPPLTINGKDTVDERKVEAAELIMHWSLEPNHKEAKENSLSPLKTSENLEESLQFKENESSHKVQLNITLQSRNAWRRSQFFLQPVDTECTENSPALVPSLNSPNRFCTWDPEEERKRQEKWQQEQERLLQERYQREQEKLKEEWEKAQKEVEEEERKYYEEERKIIEDTVVPLTLSPNSNAQFNISERNGNFIPALTEHVKRDQENNNEKLLTESSRTIKNETHMHSNVTQQLLLHTQEGSILGAKEKQIKGKTDKWEAEQSICIMDREELSNHVQSNECNTPTRLPLQAGCKQVTSSESSLPGDLGSPKNPITEKDAIQNMSSIIPTRGTDFKEDTMNSSQSSTSQCQSPNSVDCVRDNLGMRSPAQTSEFEMAY

Target ORF information:

RefSeq Version	XM_004911221.3
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis LIM and calponin homology domains 1 (limch1), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004911221.3

ORF Insert Sequence:

ATGGCCAGCT CCGCCACACT GCAAGGGGAA GAGATCCCCC CGCCCCAGGA GTCTCCCCCA 
GACACGGCTT GCAGGGAGGC ACAGAAGTGG ATTGAGCAAG TTACTGGCAA AAGCTTTGGC 
GACAAAGACT TCAGGACTGG ACTGGAGAAT GGAATCCTTC TGTGCGAGTT ATTAAATGCT 
ATTAAACCAG GACTCGTAAA AAAGATCAAC AGACTTCCTA CTCCCATTGC TGGATTGGAC 
AATATAACAT TATTCCTTAG AGGCTGCAAA GAGCTCGGGC TGAAAGAATC TCAGTTGTTT 
GACCCAGGTG ACCTACAGGA CACAGCAAAC AGGACAACAG CCAGGAATTC AGATTGCAAC 
AGAAAACTAA GAAATGTACT AATCACCGTG TACTGGCTGG GGAAAGCTGC AAGCAGCTGC 
TCGTCCTATA GTGGCAACAA CCTGGACTTA AAGGAGTTTG AGGGATTGCT GGCACAAATG 
AGAAAAGAAA ATGAGGAAAT AGAGAGTCCA AAACGCAGCA TACGGGACAG TGGCTACATA 
GATTGTTGGG ACTCTGAGAG AAGTGACTCC CTTTCTCCTC CCAGACATGG TAGAGATGAC 
TCCTTCGATA GTTTAGACTC ATTTGGATCT AGATCACAAC AGACACCATC TCCAGATGTA 
GTGCTGAGGG GAAGCAGCGA CGGCAGAGGA AGCGACTCTG AGTCTGACCT ACCTCACAGA 
AGGTTACCTG ATGTGCGAAA AGATGATATG TCTGCTAGAC GGGTATCCTA CGGTGAAAAC 
AAAGCATTTG TGCCTTTCAA CCAATACCTC CCCAACAAGA GCAACCAGAC CTCCTACATC 
CCGGCACCCC TGAGGAAGAA GAAGGCTGAG CGTGAGGACT ACCGGAAAAG CTGGAGCACA 
GCTACATCCC CCCTGGGGGG AGAGCGACCC TTCAGTCCCA AACAAAGTCC AGTCATTAAA 
GAAGAACAAA ACGAGTTGCA GTGTGAAGGA GAAGGGACCA AGCAGATGAT TGCCTTAAAA 
GAACCTTGCC TTGGAGCAGA AAATAGGAGT GAGAGAGTTG GTGAAAGTGA AGGGGGTGTC 
ATGGGACAAT CATCTGCTAA TGATGACATT CCAAAAGATG CAGAGGAAGT GAGAAAGCGC 
AAGCACCTTC AGAAAGCGGG TATCAAGGTC TTGCCCGCAA CAAAGCGCTA CAGCAGTCCA 
AGTCAATTTG CTGAAGACAT TGATGGCGCT CCAGACATTG TGCTGCGAAA AGAGAACACC 
TTCCTGAAGT CATATGAAGG GATGGGCTCT GATTCCGAAG AAGAAGAAGA GTGCAAAGTA 
CCTGATATAC AGCGAGATGA CTTGGCCACA AGGCGTGCAA GGATGAACCA ACCAAAAAGC 
CCAATGCAGT TTAATCAGTA TATACAGGGG TCATACTTAA ACAAAAACAG AGCATCAAGA 
AAGCTGAACA GTTCTAAGAC TTCCACTCCA GCTTCTCCAA AAATGTCTCA AGATATCAGG 
GAGTACCAAG GCCCCCAGTT ATTAAAGCCA TCACACGTCT GCAAAGAAAA GACATTTCCA 
ATACCCTCAG ACAATAAAGA GGATGCAGAA GCAGAGCAAG AAAAAGTTCC CGACATTCAG 
AAAGACGACC TGGCGAAACG CAAAGCTAAA GCTTCAGTTT CACTGCCAAA GGAACCATCG 
GCCTTTGCAG CGGTTTCAAT TACAAAGGCC GACATGGAAA CTTGGCAGAG GCTTCGAATG 
TCTTCTGAAG ATAGTGAGCA CTTGAATGAC AGGCAGGCTG ACCAGCAAGA GCAAACATCC 
AAGATTGATG TGACTAGAGC GACAAATGAT GACTTTGGGA GGAGGAAATT GTCTCTGCAA 
GAGAAAAGGG CTCGAAACAA ACAGAAGTTT GTACGCTTTG GCCCAGTGAC TGAAATTGAT 
CAGCAGAAGT GGGATAGGCT TTGTATAGCC AAGGCTGAAT CTGGCCAGGA AAGTGAAGAG 
ATGAAGGGCA GCATTACGTC TGATGAAACT GCCAGCCACA TATCTCCCTC AGCTTCTGCT 
CAGCGCTCCC AACCCCAGGA GGATGTTGGC ACAGGGCCAG TCTTGCCACA TGAGTCAAGA 
TCTTTTACAC CAGAGAGATT GGTTTCACCT GCTCCTCCTG AAAATGAAAG TGAAATTCAG 
CTCCTAGAAA CACAGCAAGA TGACATGATG GCAAGGAGAA CAGGTACATT CCAAAAATGC 
ACCAGAACCA GCAGAGACTC TCCTGTGTCC CACCAATATG CACTCCATAG CCAACAACAC 
GAAGAGAAGT CTGACTCGCT GGAACTCGAA GAGAAACAAG GACTCTTTCT GGATGTTAAC 
CCACAACTGA GAAAATTACT GCTGTCGGAC TTGGCAAATA CTAGTTCAAA AGCAACTAGC 
CCTACTTTGG GGAGCAAGTC TCTGAAAGTC ACCAAAGAAG CTGAAGATGT AAAAAATGAC 
ATGAAAGAAG GTGCCTTGAA CACAGGAAAG GATCCAAACT CTGTTATCCC AGAGGATAAC 
ACAATTGCAC CAGACCCAGA AAAAGATGAC ATGATGACAA GAAAAATCTC AGTGAGCCAG 
AAGCCATCTT GCGTCAGCCT TGGACACTTC CTTCCTGTTC CTTTTTCTTT GCAGCACAGA 
GAGGGGGAAA AGAAACCAAC AAGCTGCATC ATATCAAGCA CCAAAAAGTC CAAAAATGCA 
TCCAAAGCTG TTCCAGCCGA CATTTCTGAT GTTCCTTCTA AGCCCCTTGA CCAGAAAGAA 
ACAAAAGAAC ATGGTGAATT AGGAGATACG ACTTGCAAGC AAGCCTCCAA TAGTGTGGAG 
ACTCCCATGT GTACAGAGTT AGACAGCCAG CAACAGGATG GTCTTGAGAG GAGAATGATA 
CTGTCTACTG ACTTGGCCAA TACTGAGTTA AAAATAACTC CTCCTACTTT GGCCAACAAC 
TCTCCAACCA TAACAAAAGT ACAAGAGCCA GATGCAACCA TCTCAGAGGA CAATACAACT 
GCACCAAACC CTGAAAAAGA TGATATGATG ACCAGAAGAA TCTATGTAAC CCAGAAACCA 
TCGTGTGTAG GTCTTGGCCA CTTCCTTCCA GTTCCATTTT CTTTGCAGCA AAAAGTTGAG 
GAAAAGAAAT CAACGAGCTC CACTCTATCG ATCAAAATAA AGTCAGATAA TTGCACCCAA 
GCACTTGCAG TCGGCAGTTC TGACCTTCCT TGTAAGCTCC TCGGCCAATG TGAAACAGAG 
GGACATGTTG AATTACGATC CGATGTGACT TGCGATCAAA CCTCCACTGA GATCATTAGT 
GATGGGATTC CCACTGGTAA AGGGTTAGAT ACCCAGGCAG AAGACGCTCT TGAGACATGT 
GACCATGAGT TATTCAGATT CAGCCCAAGA TCTGTTCTGT CTGATGATGG AGAGAGCATG 
AGCATGTGCG ACATGAGGTA CGAAGAAGAG GGGAGTATGC AGCCTCACAG CAGGGCACAC 
TATGAGCATC TACAGCATGT CAACAGCTTG CTCAAGGAGG AGGATGACAC ATGGCAAGAT 
GACCTAGCTA AGTGGAAGAC ACGGAGAAGA AGTGCATCAC AGGATCTGAT CAAAAAAGAG 
GAACAGAGGA AGAAGATGGA GAGAATGATG AGCGGAGAGG ATGAACTGTC GGAGCGTAGG 
AAAAGCATAA AAACATATAG AGAGATTGTG GAAGAAAAAG AACGTCGAGA GAAAGAACTG 
CATGAAGCCT ACAAAAATGC CAAGTCAAGA GATGAGGCTG AACACATATT ACAGAGCTAC 
ATTGAAAAGT TTACAATTAG TGAAGCTGTT CTTGAGGGAT TAGTAATGCC AAAAATCTTA 
GAAAGAAGCC AGTCGGTGGA GCCAAGTTTA TTGTCTTCAT CAGAGGACCC AAACATCCTG 
AAATACCTGA GGCAACAGTC TTTACCATCA CCAAAATACA CTTCCACTGT TGAAGCAACT 
GTCTCCCCCA GCAGCCCATG TGAAGCAAAA AGTCCAACAG GTCTGATATC ACCAAGCAAA 
AACATTATCT CAAAGACTGT GCCTATGCTA ACAGCCAAGC CTTACTCCCA GCCCAAAAAC 
ACCCAGGAAG TCCTCAAGAC ATTTAAGGTA GATGGAAAGG TAAACCTCAA CGGGGTAGAA 
GAGCAGAAAG AAGAGGAACA TGGAAACCAG ACATTTGCCC CATCACTCAC CCGATCTCAG 
ATGTTTGAAG GTGTAGCCAG AGTTGATGTT CCTGTTGTTG AAATAAAGCA AGACGTAACT 
TCCATAGAGC TTAATCTGGC CAGACCAAAC ACCCTGAATT CCTTACAAGA ACCCAAAAGT 
CTCTGCGAAG ACAGAAATCA CATAAGCCAG GAGGATGAGT CAGATTCTCT GGTGCAATTA 
GAAAGAGCTC ACTCCGTGGA AATATTGTCA GAAGGTAACA CCCAATCAAG CAATGCAACC 
CAGGATGTAC CTGTTGTGAG CAGTATAGAG ATTCCACCAT TGACCATCAA TGGAAAGGAC 
ACTGTGGATG AGAGGAAAGT GGAAGCTGCC GAGTTGATCA TGCATTGGTC CCTGGAGCCG 
AATCATAAGG AAGCCAAAGA AAATTCTTTA TCGCCTTTAA AAACTTCTGA AAATTTAGAA 
GAATCCTTGC AGTTTAAAGA GAATGAATCA TCACACAAAG TGCAGCTAAA CATTACGCTT 
CAGAGCCGGA ACGCCTGGAG ACGCTCCCAG TTTTTTTTGC AGCCAGTGGA TACAGAATGT 
ACAGAAAATT CACCAGCACT TGTACCCAGT TTGAATTCTC CTAACCGATT CTGCACATGG 
GACCCTGAAG AAGAACGCAA ACGGCAAGAA AAATGGCAGC AGGAGCAGGA AAGGCTTCTC 
CAGGAGAGGT ACCAGAGAGA GCAGGAAAAA CTTAAAGAAG AATGGGAAAA AGCTCAGAAA 
GAAGTAGAAG AGGAGGAAAG GAAATATTAT GAGGAGGAAA GAAAGATAAT TGAAGACACT 
GTGGTCCCAC TGACTTTGTC TCCAAATTCT AATGCACAGT TTAATATCTC TGAAAGAAAT 
GGGAACTTCA TTCCAGCACT TACAGAACAT GTCAAGAGAG ATCAAGAAAA TAACAATGAA 
AAGCTCTTGA CAGAGTCCAG CAGAACGATA AAGAATGAGA CACATATGCA CAGCAATGTA 
ACACAGCAAC TGCTTTTGCA TACACAGGAG GGGTCAATTC TTGGTGCTAA AGAGAAACAA 
ATAAAGGGAA AAACAGACAA GTGGGAAGCA GAACAGTCCA TCTGTATTAT GGACCGAGAG 
GAGCTGAGCA ACCATGTGCA GTCTAATGAA TGTAATACAC CCACTAGGTT ACCCTTGCAG 
GCAGGATGTA AACAAGTGAC GTCATCAGAG TCAAGTCTGC CTGGGGATCT TGGATCACCA 
AAAAATCCTA TCACTGAAAA AGATGCTATT CAGAATATGT CGTCCATAAT ACCCACACGG 
GGAACTGATT TCAAGGAAGA TACAATGAAT TCTTCACAGT CCTCTACATC ACAGTGCCAG 
TCACCAAACA GTGTGGACTG TGTAAGGGAC AACTTGGGGA TGCGATCACC GGCACAGACG 
TCAGAATTCG AAATGGCCTA CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

limch1 ( XM_012955695.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012811149.1 Xenopus tropicalis LIM and calponin homology domains 1 (limch1), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OXa30867
Clone ID Related Accession (Same CDS sequence)	XM_012955695.2
Accession Version	XM_012955695.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5700bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1473177600000
Organism	Xenopus tropicalis(tropical clawed frog)
Product	LIM and calponin homology domains-containing protein 1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012955695.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTGAAGA CGCCAAAAGC TCAAGTTACT GGCAAAAGCT TTGGCGACAA AGACTTCAGG 
ACTGGACTGG AGAATGGAAT CCTTCTGTGC GAGTTATTAA ATGCTATTAA ACCAGGACTC 
GTAAAAAAGA TCAACAGACT TCCTACTCCC ATTGCTGGAT TGGACAATAT AACATTATTC 
CTTAGAGGCT GCAAAGAGCT CGGGCTGAAA GAATCTCAGT TGTTTGACCC AGGTGACCTA 
CAGGACACAG CAAACAGGAC AACAGCCAGG AATTCAGATT GCAACAGAAA ACTAAGAAAT 
GTACTAATCA CCGTGTACTG GCTGGGGAAA GCTGCAAGCA GCTGCTCGTC CTATAGTGGC 
AACAACCTGG ACTTAAAGGA GTTTGAGGGA TTGCTGGCAC AAATGAGAAA AGAAAATGAG 
GAAATAGAGA GTCCAAAACG CAGCATACGG GACAGTGGCT ACATAGATTG TTGGGACTCT 
GAGAGAAGTG ACTCCCTTTC TCCTCCCAGA CATGGTAGAG ATGACTCCTT CGATAGTTTA 
GACTCATTTG GATCTAGATC ACAACAGACA CCATCTCCAG ATGTAGTGCT GAGGGGAAGC 
AGCGACGGCA GAGGAAGCGA CTCTGAGTCT GACCTACCTC ACAGAAGGTT ACCTGATGTG 
CGAAAAGATG ATATGTCTGC TAGACGGGTA TCCTACGGTG AAAACAAAGC ATTTGTGCCT 
TTCAACCAAT ACCTCCCCAA CAAGAGCAAC CAGACCTCCT ACATCCCGGC ACCCCTGAGG 
AAGAAGAAGG CTGAGCGTGA GGACTACCGG AAAAGCTGGA GCACAGCTAC ATCCCCCCTG 
GGGGGAGAGC GACCCTTCAG TCCCAAACAA AGTCCAGTCA TTAAAGAAGA ACAAAACGAG 
TTGCAGTGTG AAGGAGAAGG GACCAAGCAG ATGATTGCCT TAAAAGAACC TTGCCTTGGA 
GCAGAAAATA GGAGTGAGAG AGTTGGTGAA AGTGAAGGGG GTGTCATGGG ACAATCATCT 
GCTAATGATG ACATTCCAAA AGATGCAGAG GAAGTGAGAA AGCGCAAGCA CCTTCAGAAA 
GCGGGTATCA AGGTCTTGCC CGCAACAAAG CGCTACAGCA GTCCAAGTCA ATTTGCTGAA 
GACATTGATG GCGCTCCAGA CATTGTGCTG CGAAAAGAGA ACACCTTCCT GAAGTCATAT 
GAAGGGATGG GCTCTGATTC CGAAGAAGAA GAAGAGTGCA AAGTACCTGA TATACAGCGA 
GATGACTTGG CCACAAGGCG TGCAAGGATG AACCAACCAA AAAGCCCAAT GCAGTTTAAT 
CAGTATATAC AGGGGTCATA CTTAAACAAA AACAGAGCAT CAAGAAAGCT GAACAGTTCT 
AAGACTTCCA CTCCAGCTTC TCCAAAAATG TCTCAAGATA TCAGGGAGTA CCAAGGCCCC 
CAGTTATTAA AGCCATCACA CGTCTGCAAA GAAAAGACAT TTCCAATACC CTCAGACAAT 
AAAGAGGATG CAGAAGCAGA GCAAGAAAAA GTTCCCGACA TTCAGAAAGA CGACCTGGCG 
AAACGCAAAG CTAAAGCTTC AGTTTCACTG CCAAAGGAAC CATCGGCCTT TGCAGCGGTT 
TCAATTACAA AGGCCGACAT GGAAACTTGG CAGAGGCTTC GAATGTCTTC TGAAGATAGT 
GAGCACTTGA ATGACAGGCA GGCTGACCAG CAAGAGCAAA CATCCAAGAT TGATGTGACT 
AGAGCGACAA ATGATGACTT TGGGAGGAGG AAATTGTCTC TGCAAGAGAA AAGGGCTCGA 
AACAAACAGA AGTTTGTACG CTTTGGCCCA GTGACTGAAA TTGATCAGCA GAAGTGGGAT 
AGGCTTTGTA TAGCCAAGGC TGAATCTGGC CAGGAAAGTG AAGAGATGAA GGGCAGCATT 
ACGTCTGATG AAACTGCCAG CCACATATCT CCCTCAGCTT CTGCTCAGCG CTCCCAACCC 
CAGGAGGATG TTGGCACAGG GCCAGTCTTG CCACATGAGT CAAGATCTTT TACACCAGAG 
AGATTGGTTT CACCTGCTCC TCCTGAAAAT GAAAGTGAAA TTCAGCTCCT AGAAACACAG 
CAAGATGACA TGATGGCAAG GAGAACAGGT ACATTCCAAA AATGCACCAG AACCAGCAGA 
GACTCTCCTG TGTCCCACCA ATATGCACTC CATAGCCAAC AACACGAAGA GAAGTCTGAC 
TCGCTGGAAC TCGAAGAGAA ACAAGGACTC TTTCTGGATG TTAACCCACA ACTGAGAAAA 
TTACTGCTGT CGGACTTGGC AAATACTAGT TCAAAAGCAA CTAGCCCTAC TTTGGGGAGC 
AAGTCTCTGA AAGTCACCAA AGAAGCTGAA GATGTAAAAA ATGACATGAA AGAAGGTGCC 
TTGAACACAG GAAAGGATCC AAACTCTGTT ATCCCAGAGG ATAACACAAT TGCACCAGAC 
CCAGAAAAAG ATGACATGAT GACAAGAAAA ATCTCAGTGA GCCAGAAGCC ATCTTGCGTC 
AGCCTTGGAC ACTTCCTTCC TGTTCCTTTT TCTTTGCAGC ACAGAGAGGG GGAAAAGAAA 
CCAACAAGCT GCATCATATC AAGCACCAAA AAGTCCAAAA ATGCATCCAA AGCTGTTCCA 
GCCGACATTT CTGATGTTCC TTCTAAGCCC CTTGACCAGA AAGAAACAAA AGAACATGGT 
GAATTAGGAG ATACGACTTG CAAGCAAGCC TCCAATAGTG TGGAGACTCC CATGTGTACA 
GAGTTAGACA GCCAGCAACA GGATGGTCTT GAGAGGAGAA TGATACTGTC TACTGACTTG 
GCCAATACTG AGTTAAAAAT AACTCCTCCT ACTTTGGCCA ACAACTCTCC AACCATAACA 
AAAGTACAAG AGCCAGATGC AACCATCTCA GAGGACAATA CAACTGCACC AAACCCTGAA 
AAAGATGATA TGATGACCAG AAGAATCTAT GTAACCCAGA AACCATCGTG TGTAGGTCTT 
GGCCACTTCC TTCCAGTTCC ATTTTCTTTG CAGCAAAAAG TTGAGGAAAA GAAATCAACG 
AGCTCCACTC TATCGATCAA AATAAAGTCA GATAATTGCA CCCAAGCACT TGCAGTCGGC 
AGTTCTGACC TTCCTTGTAA GCTCCTCGGC CAATGTGAAA CAGAGGGACA TGTTGAATTA 
CGATCCGATG TGACTTGCGA TCAAACCTCC ACTGAGATCA TTAGTGATGG GATTCCCACT 
GGTAAAGGGT TAGATACCCA GGCAGAAGAC GCTCTTGAGA CATGTGACCA TGAGTTATTC 
AGATTCAGCC CAAGATCTGT TCTGTCTGAT GATGGAGAGA GCATGAGCAT GTGCGACATG 
AGGTACGAAG AAGAGGGGAG TATGCAGCCT CACAGCAGGG CACACTATGA GCATCTACAG 
CATGTCAACA GCTTGCTCAA GGAGGAGGAT GACACATGGC AAGATGACCT AGCTAAGTGG 
AAGACACGGA GAAGAAGTGC ATCACAGGAT CTGATCAAAA AAGAGGAACA GAGGAAGAAG 
ATGGAGAGAA TGATGAGCGG AGAGGATGAA CTGTCGGAGC GTAGGAAAAG CATAAAAACA 
TATAGAGAGA TTGTGGAAGA AAAAGAACGT CGAGAGAAAG AACTGCATGA AGCCTACAAA 
AATGCCAAGT CAAGAGATGA GGCTGAACAC ATATTACAGA GCTACATTGA AAAGTTTACA 
ATTAGTGAAG CTGTTCTTGA GGGATTAGTA ATGCCAAAAA TCTTAGAAAG AAGCCAGTCG 
GTGGAGCCAA GTTTATTGTC TTCATCAGAG GACCCAAACA TCCTGAAATA CCTGAGGCAA 
CAGTCTTTAC CATCACCAAA ATACACTTCC ACTGTTGAAG CAACTGTCTC CCCCAGCAGC 
CCATGTGAAG CAAAAAGTCC AACAGGTCTG ATATCACCAA GCAAAAACAT TATCTCAAAG 
ACTGTGCCTA TGCTAACAGC CAAGCCTTAC TCCCAGCCCA AAAACACCCA GGAAGTCCTC 
AAGACATTTA AGGTAGATGG AAAGGTAAAC CTCAACGGGG TAGAAGAGCA GAAAGAAGAG 
GAACATGGAA ACCAGACATT TGCCCCATCA CTCACCCGAT CTCAGATGTT TGAAGGTGTA 
GCCAGAGTTG ATGTTCCTGT TGTTGAAATA AAGCAAGACG TAACTTCCAT AGAGCTTAAT 
CTGGCCAGAC CAAACACCCT GAATTCCTTA CAAGAACCCA AAAGTCTCTG CGAAGACAGA 
AATCACATAA GCCAGGAGGA TGAGTCAGAT TCTCTGGTGC AATTAGAAAG AGCTCACTCC 
GTGGAAATAT TGTCAGAAGG TAACACCCAA TCAAGCAATG CAACCCAGGA TGTACCTGTT 
GTGAGCAGTA TAGAGATTCC ACCATTGACC ATCAATGGAA AGGACACTGT GGATGAGAGG 
AAAGTGGAAG CTGCCGAGTT GATCATGCAT TGGTCCCTGG AGCCGAATCA TAAGGAAGCC 
AAAGAAAATT CTTTATCGCC TTTAAAAACT TCTGAAAATT TAGAAGAATC CTTGCAGTTT 
AAAGAGAATG AATCATCACA CAAAGTGCAG CTAAACATTA CGCTTCAGAG CCGGAACGCC 
TGGAGACGCT CCCAGTTTTT TTTGCAGCCA GTGGATACAG AATGTACAGA AAATTCACCA 
GCACTTGTAC CCAGTTTGAA TTCTCCTAAC CGATTCTGCA CATGGGACCC TGAAGAAGAA 
CGCAAACGGC AAGAAAAATG GCAGCAGGAG CAGGAAAGGC TTCTCCAGGA GAGGTACCAG 
AGAGAGCAGG AAAAACTTAA AGAAGAATGG GAAAAAGCTC AGAAAGAAGT AGAAGAGGAG 
GAAAGGAAAT ATTATGAGGA GGAAAGAAAG ATAATTGAAG ACACTGTGGT CCCACTGACT 
TTGTCTCCAA ATTCTAATGC ACAGTTTAAT ATCTCTGAAA GAAATGGGAA CTTCATTCCA 
GCACTTACAG AACATGTCAA GAGAGATCAA GAAAATAACA ATGAAAAGCT CTTGACAGAG 
TCCAGCAGAA CGATAAAGAA TGAGACACAT ATGCACAGCA ATGTAACACA GCAACTGCTT 
TTGCATACAC AGGAGGGGTC AATTCTTGGT GCTAAAGAGA AACAAATAAA GGGAAAAACA 
GACAAGTGGG AAGCAGAACA GTCCATCTGT ATTATGGACC GAGAGGAGCT GAGCAACCAT 
GTGCAGTCTA ATGAATGTAA TACACCCACT AGGTTACCCT TGCAGGCAGG ATGTAAACAA 
GTGACGTCAT CAGAGTCAAG TCTGCCTGGG GATCTTGGAT CACCAAAAAA TCCTATCACT 
GAAAAAGATG CTATTCAGAA TATGTCGTCC ATAATACCCA CACGGGGAAC TGATTTCAAG 
GAAGATACAA TGAATTCTTC ACAGTCCTCT ACATCACAGT GCCAGTCACC AAACAGGTCT 
GTTAGTGGGA AAAAACTGTG CTCCACATGT GGACTTCCTC TAGGCAAGGG AGCTGCCATG 
ATAATTGAAA CCCTCAGTCT TTATTTCCAC ATCCAGTGCT TCAAGTGTGG ACTGTGTAAG 
GGACAACTTG GGGATGCGAT CACCGGCACA GACGTCAGAA TTCGAAATGG CCTACTGAAC 
TGCAATGACT GTTACATGAA GTCTAGAAGT AAGGCGGCTG GCCACCCCAC CCCTTTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012811149.1
CDS	193..5892
Translation	MLKTPKAQVTGKSFGDKDFRTGLENGILLCELLNAIKPGLVKKINRLPTPIAGLDNITLFLRGCKELGLKESQLFDPGDLQDTANRTTARNSDCNRKLRNVLITVYWLGKAASSCSSYSGNNLDLKEFEGLLAQMRKENEEIESPKRSIRDSGYIDCWDSERSDSLSPPRHGRDDSFDSLDSFGSRSQQTPSPDVVLRGSSDGRGSDSESDLPHRRLPDVRKDDMSARRVSYGENKAFVPFNQYLPNKSNQTSYIPAPLRKKKAEREDYRKSWSTATSPLGGERPFSPKQSPVIKEEQNELQCEGEGTKQMIALKEPCLGAENRSERVGESEGGVMGQSSANDDIPKDAEEVRKRKHLQKAGIKVLPATKRYSSPSQFAEDIDGAPDIVLRKENTFLKSYEGMGSDSEEEEECKVPDIQRDDLATRRARMNQPKSPMQFNQYIQGSYLNKNRASRKLNSSKTSTPASPKMSQDIREYQGPQLLKPSHVCKEKTFPIPSDNKEDAEAEQEKVPDIQKDDLAKRKAKASVSLPKEPSAFAAVSITKADMETWQRLRMSSEDSEHLNDRQADQQEQTSKIDVTRATNDDFGRRKLSLQEKRARNKQKFVRFGPVTEIDQQKWDRLCIAKAESGQESEEMKGSITSDETASHISPSASAQRSQPQEDVGTGPVLPHESRSFTPERLVSPAPPENESEIQLLETQQDDMMARRTGTFQKCTRTSRDSPVSHQYALHSQQHEEKSDSLELEEKQGLFLDVNPQLRKLLLSDLANTSSKATSPTLGSKSLKVTKEAEDVKNDMKEGALNTGKDPNSVIPEDNTIAPDPEKDDMMTRKISVSQKPSCVSLGHFLPVPFSLQHREGEKKPTSCIISSTKKSKNASKAVPADISDVPSKPLDQKETKEHGELGDTTCKQASNSVETPMCTELDSQQQDGLERRMILSTDLANTELKITPPTLANNSPTITKVQEPDATISEDNTTAPNPEKDDMMTRRIYVTQKPSCVGLGHFLPVPFSLQQKVEEKKSTSSTLSIKIKSDNCTQALAVGSSDLPCKLLGQCETEGHVELRSDVTCDQTSTEIISDGIPTGKGLDTQAEDALETCDHELFRFSPRSVLSDDGESMSMCDMRYEEEGSMQPHSRAHYEHLQHVNSLLKEEDDTWQDDLAKWKTRRRSASQDLIKKEEQRKKMERMMSGEDELSERRKSIKTYREIVEEKERREKELHEAYKNAKSRDEAEHILQSYIEKFTISEAVLEGLVMPKILERSQSVEPSLLSSSEDPNILKYLRQQSLPSPKYTSTVEATVSPSSPCEAKSPTGLISPSKNIISKTVPMLTAKPYSQPKNTQEVLKTFKVDGKVNLNGVEEQKEEEHGNQTFAPSLTRSQMFEGVARVDVPVVEIKQDVTSIELNLARPNTLNSLQEPKSLCEDRNHISQEDESDSLVQLERAHSVEILSEGNTQSSNATQDVPVVSSIEIPPLTINGKDTVDERKVEAAELIMHWSLEPNHKEAKENSLSPLKTSENLEESLQFKENESSHKVQLNITLQSRNAWRRSQFFLQPVDTECTENSPALVPSLNSPNRFCTWDPEEERKRQEKWQQEQERLLQERYQREQEKLKEEWEKAQKEVEEEERKYYEEERKIIEDTVVPLTLSPNSNAQFNISERNGNFIPALTEHVKRDQENNNEKLLTESSRTIKNETHMHSNVTQQLLLHTQEGSILGAKEKQIKGKTDKWEAEQSICIMDREELSNHVQSNECNTPTRLPLQAGCKQVTSSESSLPGDLGSPKNPITEKDAIQNMSSIIPTRGTDFKEDTMNSSQSSTSQCQSPNRSVSGKKLCSTCGLPLGKGAAMIIETLSLYFHIQCFKCGLCKGQLGDAITGTDVRIRNGLLNCNDCYMKSRSKAAGHPTPL

Target ORF information:

RefSeq Version	XM_012955695.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis LIM and calponin homology domains 1 (limch1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012955695.2

ORF Insert Sequence:

ATGTTGAAGA CGCCAAAAGC TCAAGTTACT GGCAAAAGCT TTGGCGACAA AGACTTCAGG 
ACTGGACTGG AGAATGGAAT CCTTCTGTGC GAGTTATTAA ATGCTATTAA ACCAGGACTC 
GTAAAAAAGA TCAACAGACT TCCTACTCCC ATTGCTGGAT TGGACAATAT AACATTATTC 
CTTAGAGGCT GCAAAGAGCT CGGGCTGAAA GAATCTCAGT TGTTTGACCC AGGTGACCTA 
CAGGACACAG CAAACAGGAC AACAGCCAGG AATTCAGATT GCAACAGAAA ACTAAGAAAT 
GTACTAATCA CCGTGTACTG GCTGGGGAAA GCTGCAAGCA GCTGCTCGTC CTATAGTGGC 
AACAACCTGG ACTTAAAGGA GTTTGAGGGA TTGCTGGCAC AAATGAGAAA AGAAAATGAG 
GAAATAGAGA GTCCAAAACG CAGCATACGG GACAGTGGCT ACATAGATTG TTGGGACTCT 
GAGAGAAGTG ACTCCCTTTC TCCTCCCAGA CATGGTAGAG ATGACTCCTT CGATAGTTTA 
GACTCATTTG GATCTAGATC ACAACAGACA CCATCTCCAG ATGTAGTGCT GAGGGGAAGC 
AGCGACGGCA GAGGAAGCGA CTCTGAGTCT GACCTACCTC ACAGAAGGTT ACCTGATGTG 
CGAAAAGATG ATATGTCTGC TAGACGGGTA TCCTACGGTG AAAACAAAGC ATTTGTGCCT 
TTCAACCAAT ACCTCCCCAA CAAGAGCAAC CAGACCTCCT ACATCCCGGC ACCCCTGAGG 
AAGAAGAAGG CTGAGCGTGA GGACTACCGG AAAAGCTGGA GCACAGCTAC ATCCCCCCTG 
GGGGGAGAGC GACCCTTCAG TCCCAAACAA AGTCCAGTCA TTAAAGAAGA ACAAAACGAG 
TTGCAGTGTG AAGGAGAAGG GACCAAGCAG ATGATTGCCT TAAAAGAACC TTGCCTTGGA 
GCAGAAAATA GGAGTGAGAG AGTTGGTGAA AGTGAAGGGG GTGTCATGGG ACAATCATCT 
GCTAATGATG ACATTCCAAA AGATGCAGAG GAAGTGAGAA AGCGCAAGCA CCTTCAGAAA 
GCGGGTATCA AGGTCTTGCC CGCAACAAAG CGCTACAGCA GTCCAAGTCA ATTTGCTGAA 
GACATTGATG GCGCTCCAGA CATTGTGCTG CGAAAAGAGA ACACCTTCCT GAAGTCATAT 
GAAGGGATGG GCTCTGATTC CGAAGAAGAA GAAGAGTGCA AAGTACCTGA TATACAGCGA 
GATGACTTGG CCACAAGGCG TGCAAGGATG AACCAACCAA AAAGCCCAAT GCAGTTTAAT 
CAGTATATAC AGGGGTCATA CTTAAACAAA AACAGAGCAT CAAGAAAGCT GAACAGTTCT 
AAGACTTCCA CTCCAGCTTC TCCAAAAATG TCTCAAGATA TCAGGGAGTA CCAAGGCCCC 
CAGTTATTAA AGCCATCACA CGTCTGCAAA GAAAAGACAT TTCCAATACC CTCAGACAAT 
AAAGAGGATG CAGAAGCAGA GCAAGAAAAA GTTCCCGACA TTCAGAAAGA CGACCTGGCG 
AAACGCAAAG CTAAAGCTTC AGTTTCACTG CCAAAGGAAC CATCGGCCTT TGCAGCGGTT 
TCAATTACAA AGGCCGACAT GGAAACTTGG CAGAGGCTTC GAATGTCTTC TGAAGATAGT 
GAGCACTTGA ATGACAGGCA GGCTGACCAG CAAGAGCAAA CATCCAAGAT TGATGTGACT 
AGAGCGACAA ATGATGACTT TGGGAGGAGG AAATTGTCTC TGCAAGAGAA AAGGGCTCGA 
AACAAACAGA AGTTTGTACG CTTTGGCCCA GTGACTGAAA TTGATCAGCA GAAGTGGGAT 
AGGCTTTGTA TAGCCAAGGC TGAATCTGGC CAGGAAAGTG AAGAGATGAA GGGCAGCATT 
ACGTCTGATG AAACTGCCAG CCACATATCT CCCTCAGCTT CTGCTCAGCG CTCCCAACCC 
CAGGAGGATG TTGGCACAGG GCCAGTCTTG CCACATGAGT CAAGATCTTT TACACCAGAG 
AGATTGGTTT CACCTGCTCC TCCTGAAAAT GAAAGTGAAA TTCAGCTCCT AGAAACACAG 
CAAGATGACA TGATGGCAAG GAGAACAGGT ACATTCCAAA AATGCACCAG AACCAGCAGA 
GACTCTCCTG TGTCCCACCA ATATGCACTC CATAGCCAAC AACACGAAGA GAAGTCTGAC 
TCGCTGGAAC TCGAAGAGAA ACAAGGACTC TTTCTGGATG TTAACCCACA ACTGAGAAAA 
TTACTGCTGT CGGACTTGGC AAATACTAGT TCAAAAGCAA CTAGCCCTAC TTTGGGGAGC 
AAGTCTCTGA AAGTCACCAA AGAAGCTGAA GATGTAAAAA ATGACATGAA AGAAGGTGCC 
TTGAACACAG GAAAGGATCC AAACTCTGTT ATCCCAGAGG ATAACACAAT TGCACCAGAC 
CCAGAAAAAG ATGACATGAT GACAAGAAAA ATCTCAGTGA GCCAGAAGCC ATCTTGCGTC 
AGCCTTGGAC ACTTCCTTCC TGTTCCTTTT TCTTTGCAGC ACAGAGAGGG GGAAAAGAAA 
CCAACAAGCT GCATCATATC AAGCACCAAA AAGTCCAAAA ATGCATCCAA AGCTGTTCCA 
GCCGACATTT CTGATGTTCC TTCTAAGCCC CTTGACCAGA AAGAAACAAA AGAACATGGT 
GAATTAGGAG ATACGACTTG CAAGCAAGCC TCCAATAGTG TGGAGACTCC CATGTGTACA 
GAGTTAGACA GCCAGCAACA GGATGGTCTT GAGAGGAGAA TGATACTGTC TACTGACTTG 
GCCAATACTG AGTTAAAAAT AACTCCTCCT ACTTTGGCCA ACAACTCTCC AACCATAACA 
AAAGTACAAG AGCCAGATGC AACCATCTCA GAGGACAATA CAACTGCACC AAACCCTGAA 
AAAGATGATA TGATGACCAG AAGAATCTAT GTAACCCAGA AACCATCGTG TGTAGGTCTT 
GGCCACTTCC TTCCAGTTCC ATTTTCTTTG CAGCAAAAAG TTGAGGAAAA GAAATCAACG 
AGCTCCACTC TATCGATCAA AATAAAGTCA GATAATTGCA CCCAAGCACT TGCAGTCGGC 
AGTTCTGACC TTCCTTGTAA GCTCCTCGGC CAATGTGAAA CAGAGGGACA TGTTGAATTA 
CGATCCGATG TGACTTGCGA TCAAACCTCC ACTGAGATCA TTAGTGATGG GATTCCCACT 
GGTAAAGGGT TAGATACCCA GGCAGAAGAC GCTCTTGAGA CATGTGACCA TGAGTTATTC 
AGATTCAGCC CAAGATCTGT TCTGTCTGAT GATGGAGAGA GCATGAGCAT GTGCGACATG 
AGGTACGAAG AAGAGGGGAG TATGCAGCCT CACAGCAGGG CACACTATGA GCATCTACAG 
CATGTCAACA GCTTGCTCAA GGAGGAGGAT GACACATGGC AAGATGACCT AGCTAAGTGG 
AAGACACGGA GAAGAAGTGC ATCACAGGAT CTGATCAAAA AAGAGGAACA GAGGAAGAAG 
ATGGAGAGAA TGATGAGCGG AGAGGATGAA CTGTCGGAGC GTAGGAAAAG CATAAAAACA 
TATAGAGAGA TTGTGGAAGA AAAAGAACGT CGAGAGAAAG AACTGCATGA AGCCTACAAA 
AATGCCAAGT CAAGAGATGA GGCTGAACAC ATATTACAGA GCTACATTGA AAAGTTTACA 
ATTAGTGAAG CTGTTCTTGA GGGATTAGTA ATGCCAAAAA TCTTAGAAAG AAGCCAGTCG 
GTGGAGCCAA GTTTATTGTC TTCATCAGAG GACCCAAACA TCCTGAAATA CCTGAGGCAA 
CAGTCTTTAC CATCACCAAA ATACACTTCC ACTGTTGAAG CAACTGTCTC CCCCAGCAGC 
CCATGTGAAG CAAAAAGTCC AACAGGTCTG ATATCACCAA GCAAAAACAT TATCTCAAAG 
ACTGTGCCTA TGCTAACAGC CAAGCCTTAC TCCCAGCCCA AAAACACCCA GGAAGTCCTC 
AAGACATTTA AGGTAGATGG AAAGGTAAAC CTCAACGGGG TAGAAGAGCA GAAAGAAGAG 
GAACATGGAA ACCAGACATT TGCCCCATCA CTCACCCGAT CTCAGATGTT TGAAGGTGTA 
GCCAGAGTTG ATGTTCCTGT TGTTGAAATA AAGCAAGACG TAACTTCCAT AGAGCTTAAT 
CTGGCCAGAC CAAACACCCT GAATTCCTTA CAAGAACCCA AAAGTCTCTG CGAAGACAGA 
AATCACATAA GCCAGGAGGA TGAGTCAGAT TCTCTGGTGC AATTAGAAAG AGCTCACTCC 
GTGGAAATAT TGTCAGAAGG TAACACCCAA TCAAGCAATG CAACCCAGGA TGTACCTGTT 
GTGAGCAGTA TAGAGATTCC ACCATTGACC ATCAATGGAA AGGACACTGT GGATGAGAGG 
AAAGTGGAAG CTGCCGAGTT GATCATGCAT TGGTCCCTGG AGCCGAATCA TAAGGAAGCC 
AAAGAAAATT CTTTATCGCC TTTAAAAACT TCTGAAAATT TAGAAGAATC CTTGCAGTTT 
AAAGAGAATG AATCATCACA CAAAGTGCAG CTAAACATTA CGCTTCAGAG CCGGAACGCC 
TGGAGACGCT CCCAGTTTTT TTTGCAGCCA GTGGATACAG AATGTACAGA AAATTCACCA 
GCACTTGTAC CCAGTTTGAA TTCTCCTAAC CGATTCTGCA CATGGGACCC TGAAGAAGAA 
CGCAAACGGC AAGAAAAATG GCAGCAGGAG CAGGAAAGGC TTCTCCAGGA GAGGTACCAG 
AGAGAGCAGG AAAAACTTAA AGAAGAATGG GAAAAAGCTC AGAAAGAAGT AGAAGAGGAG 
GAAAGGAAAT ATTATGAGGA GGAAAGAAAG ATAATTGAAG ACACTGTGGT CCCACTGACT 
TTGTCTCCAA ATTCTAATGC ACAGTTTAAT ATCTCTGAAA GAAATGGGAA CTTCATTCCA 
GCACTTACAG AACATGTCAA GAGAGATCAA GAAAATAACA ATGAAAAGCT CTTGACAGAG 
TCCAGCAGAA CGATAAAGAA TGAGACACAT ATGCACAGCA ATGTAACACA GCAACTGCTT 
TTGCATACAC AGGAGGGGTC AATTCTTGGT GCTAAAGAGA AACAAATAAA GGGAAAAACA 
GACAAGTGGG AAGCAGAACA GTCCATCTGT ATTATGGACC GAGAGGAGCT GAGCAACCAT 
GTGCAGTCTA ATGAATGTAA TACACCCACT AGGTTACCCT TGCAGGCAGG ATGTAAACAA 
GTGACGTCAT CAGAGTCAAG TCTGCCTGGG GATCTTGGAT CACCAAAAAA TCCTATCACT 
GAAAAAGATG CTATTCAGAA TATGTCGTCC ATAATACCCA CACGGGGAAC TGATTTCAAG 
GAAGATACAA TGAATTCTTC ACAGTCCTCT ACATCACAGT GCCAGTCACC AAACAGGTCT 
GTTAGTGGGA AAAAACTGTG CTCCACATGT GGACTTCCTC TAGGCAAGGG AGCTGCCATG 
ATAATTGAAA CCCTCAGTCT TTATTTCCAC ATCCAGTGCT TCAAGTGTGG ACTGTGTAAG 
GGACAACTTG GGGATGCGAT CACCGGCACA GACGTCAGAA TTCGAAATGG CCTACTGAAC 
TGCAATGACT GTTACATGAA GTCTAGAAGT AAGGCGGCTG GCCACCCCAC CCCTTTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

limch1 ( XM_012955696.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012811150.1 Xenopus tropicalis LIM and calponin homology domains 1 (limch1), transcript variant X4, mRNA.

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CloneID	OXa30868
Clone ID Related Accession (Same CDS sequence)	XM_012955696.2
Accession Version	XM_012955696.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5694bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1473177600000
Organism	Xenopus tropicalis(tropical clawed frog)
Product	LIM and calponin homology domains-containing protein 1 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012955696.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCAGCT CCGCCACACT GCAAGGGGAA GAGATCCCCC CGCCCCAGGA GTCTCCCCCA 
GACACGGCTT GCAGGGAGGC ACAGAAGTGG ATTGAGCAAG TTACTGGCAA AAGCTTTGGC 
GACAAAGACT TCAGGACTGG ACTGGAGAAT GGAATCCTTC TGTGCGAGTT ATTAAATGCT 
ATTAAACCAG GACTCGTAAA AAAGATCAAC AGACTTCCTA CTCCCATTGC TGGATTGGAC 
AATATAACAT TATTCCTTAG AGGCTGCAAA GAGCTCGGGC TGAAAGAATC TCAGTTGTTT 
GACCCAGGTG ACCTACAGGA CACAGCAAAC AGGACAACAG CCAGGAATTC AGATTGCAAC 
AGAAAACTAA GAAATGTACT AATCACCGTG TACTGGCTGG GGAAAGCTGC AAGCAGCTGC 
TCGTCCTATA GTGGCAACAA CCTGGACTTA AAGGAGTTTG AGGGATTGCT GGCACAAATG 
AGAAAAGAAA ATGAGGAAAT AGAGAGTCCA AAACGCAGCA TACGGGACAG TGGCTACATA 
GATTGTTGGG ACTCTGAGAG AAGTGACTCC CTTTCTCCTC CCAGACATGG TAGAGATGAC 
TCCTTCGATA GTTTAGACTC ATTTGGATCT AGATCACAAC AGACACCATC TCCAGATGTA 
GTGCTGAGGG GAAGCAGCGA CGGCAGAGGA AGCGACTCTG AGTCTGACCT ACCTCACAGA 
AGGTTACCTG ATGTGCGAAA AGATGATATG TCTGCTAGAC GGGTATCCTA CGGTGAAAAC 
AAAGCATTTG TGCCTTTCAA CCAATACCTC CCCAACAAGA GCAACCAGAC CTCCTACATC 
CCGGCACCCC TGAGGAAGAA GAAGGCTGAG CGTGAGGACT ACCGGAAAAG CTGGAGCACA 
GCTACATCCC CCCTGGGGGG AGAGCGACCC TTCAGTCCCA AACAAAGTCC AGTCATTAAA 
GAAGAACAAA ACGAGTTGCA GTGTGAAGGA GAAGGGACCA AGCAGATGAT TGCCTTAAAA 
GAACCTTGCC TTGGAGCAGA AAATAGGAGT GAGAGAGTTG GTGAAAGTGA AGGGGGTGTC 
ATGGGACAAT CATCTGCTAA TGATGACATT CCAAAAGATG CAGAGGAAGT GAGAAAGCGC 
AAGCACCTTC AGAAAGCGGG TATCAAGGTC TTGCCCGCAA CAAAGCGCTA CAGCAGTCCA 
AGTCAATTTG CTGAAGACAT TGATGGCGCT CCAGACATTG TGCTGCGAAA AGAGAACACC 
TTCCTGAAGT CATATGAAGG GATGGGCTCT GATTCCGAAG AAGAAGAAGA GTGCAAAGTA 
CCTGATATAC AGCGAGATGA CTTGGCCACA AGGCGTGCAA GGATGAACCA ACCAAAAAGC 
CCAATGCAGT TTAATCAGTA TATACAGGGG TCATACTTAA ACAAAAACAG AGCATCAAGA 
AAGCTGAACA GTTCTAAGAC TTCCACTCCA GCTTCTCCAA AAATGTCTCA AGATATCAGG 
GAGTACCAAG GCCCCCAGTT ATTAAAGCCA TCACACGTCT GCAAAGAAAA GACATTTCCA 
ATACCCTCAG ACAATAAAGA GGATGCAGAA GCAGAGCAAG AAAAAGTTCC CGACATTCAG 
AAAGACGACC TGGCGAAACG CAAAGCTAAA GCTTCAGTTT CACTGCCAAA GGAACCATCG 
GCCTTTGCAG CGGTTTCAAT TACAAAGGCC GACATGGAAA CTTGGCAGAG GCTTCGAATG 
TCTTCTGAAG ATAGTGAGCA CTTGAATGAC AGGCAGGCTG ACCAGCAAGA GCAAACATCC 
AAGATTGATG TGACTAGAGC GACAAATGAT GACTTTGGGA GGAGGAAATT GTCTCTGCAA 
GAGAAAAGGG CTCGAAACAA ACAGAAGTTT GTACGCTTTG GCCCAGTGAC TGAAATTGAT 
CAGCAGAAGT GGGATAGGCT TTGTATAGCC AAGGCTGAAT CTGGCCAGGA AAGTGAAGAG 
ATGAAGGGCA GCATTACGTC TGATGAAACT GCCAGCCACA TATCTCCCTC AGCTTCTGCT 
CAGCGCTCCC AACCCCAGGA GGATGTTGGC ACAGGGCCAG TCTTGCCACA TGAGTCAAGA 
TCTTTTACAC CAGAGAGATT GGTTTCACCT GCTCCTCCTG AAAATGAAAG TGAAATTCAG 
CTCCTAGAAA CACAGCAAGA TGACATGATG GCAAGGAGAA CAGGTACATT CCAAAAATGC 
ACCAGAACCA GCAGAGACTC TCCTGTGTCC CACCAATATG CACTCCATAG CCAACAACAC 
GAAGAGAAGT CTGACTCGCT GGAACTCGAA GAGAAACAAG GACTCTTTCT GGATGTTAAC 
CCACAACTGA GAAAATTACT GCTGTCGGAC TTGGCAAATA CTAGTTCAAA AGCAACTAGC 
CCTACTTTGG GGAGCAAGTC TCTGAAAGTC ACCAAAGAAG CTGAAGATGT AAAAAATGAC 
ATGAAAGAAG GTGCCTTGAA CACAGGAAAG GATCCAAACT CTGTTATCCC AGAGGATAAC 
ACAATTGCAC CAGACCCAGA AAAAGATGAC ATGATGACAA GAAAAATCTC AGTGAGCCAG 
AAGCCATCTT GCGTCAGCCT TGGACACTTC CTTCCTGTTC CTTTTTCTTT GCAGCACAGA 
GAGGGGGAAA AGAAACCAAC AAGCTGCATC ATATCAAGCA CCAAAAAGTC CAAAAATGCA 
TCCAAAGCTG TTCCAGCCGA CATTTCTGAT GTTCCTTCTA AGCCCCTTGA CCAGAAAGAA 
ACAAAAGAAC ATGGTGAATT AGGAGATACG ACTTGCAAGC AAGCCTCCAA TAGTGTGGAG 
ACTCCCATGT GTACAGAGTT AGACAGCCAG CAACAGGATG GTCTTGAGAG GAGAATGATA 
CTGTCTACTG ACTTGGCCAA TACTGAGTTA AAAATAACTC CTCCTACTTT GGCCAACAAC 
TCTCCAACCA TAACAAAAGT ACAAGAGCCA GATGCAACCA TCTCAGAGGA CAATACAACT 
GCACCAAACC CTGAAAAAGA TGATATGATG ACCAGAAGAA TCTATGTAAC CCAGAAACCA 
TCGTGTGTAG GTCTTGGCCA CTTCCTTCCA GTTCCATTTT CTTTGCAGCA AAAAGTTGAG 
GAAAAGAAAT CAACGAGCTC CACTCTATCG ATCAAAATAA AGTCAGATAA TTGCACCCAA 
GCACTTGCAG TCGGCAGTTC TGACCTTCCT TGTAAGCTCC TCGGCCAATG TGAAACAGAG 
GGACATGTTG AATTACGATC CGATGTGACT TGCGATCAAA CCTCCACTGA GATCATTAGT 
GATGGGATTC CCACTGGTAA AGGGTTAGAT ACCCAGGCAG AAGACGCTCT TGAGACATGT 
GACCATGAGT TATTCAGATT CAGCCCAAGA TCTGTTCTGT CTGATGATGG AGAGAGCATG 
AGCATGTGCG ACATGAGGTA CGAAGAAGAG GGGAGTATGC AGCCTCACAG CAGGGCACAC 
TATGAGCATC TACAGCATGT CAACAGCTTG CTCAAGGAGG AGGATGACAC ATGGCAAGAT 
GACCTAGCTA AGTGGAAGAC ACGGAGAAGA AGTGCATCAC AGGATCTGAT CAAAAAAGAG 
GAACAGAGGA AGAAGATGGA GAGAATGATG AGCGGAGAGG ATGAACTGTC GGAGCGTAGG 
AAAAGCATAA AAACATATAG AGAGATTGTG GAAGAAAAAG AACGTCGAGA GAAAGAACTG 
CATGAAGCCT ACAAAAATGC CAAGTCAAGA GATGAGGCTG AACACATATT ACAGAGCTAC 
ATTGAAAAGT TTACAATTAG TGAAGCTGTT CTTGAGGGAT TAGTAATGCC AAAAATCTTA 
GAAAGAAGCC AGTCGGTGGA GCCAAGTTTA TTGTCTTCAT CAGAGGACCC AAACATCCTG 
AAATACCTGA GGCAACAGTC TTTACCATCA CCAAAATACA CTTCCACTGT TGAAGCAACT 
GTCTCCCCCA GCAGCCCATG TGAAGCAAAA AGTCCAACAG GTCTGATATC ACCAAGCAAA 
AACATTATCT CAAAGACTGT GCCTATGCTA ACAGCCAAGC CTTACTCCCA GCCCAAAAAC 
ACCCAGGAAG TCCTCAAGAC ATTTAAGGTA GATGGAAAGG TAAACCTCAA CGGGGTAGAA 
GAGCAGAAAG AAGAGGAACA TGGAAACCAG ACATTTGCCC CATCACTCAC CCGATCTCAG 
ATGTTTGAAG GTGTAGCCAG AGTTGATGTT CCTGTTGTTG AAATAAAGCA AGACGTAACT 
TCCATAGAGC TTAATCTGGC CAGACCAAAC ACCCTGAATT CCTTACAAGA ACCCAAAAGT 
CTCTGCGAAG ACAGAAATCA CATAAGCCAG GAGGATGAGT CAGATTCTCT GGTGCAATTA 
GAAAGAGCTC ACTCCGTGGA AATATTGTCA GAAGGTAACA CCCAATCAAG CAATGCAACC 
CAGGATGTAC CTGTTGTGAG CAGTATAGAG ATTCCACCAT TGACCATCAA TGGAAAGGAC 
ACTGTGGATG AGAGGAAAGT GGAAGCTGCC GAGTTGATCA TGCATTGGTC CCTGGAGCCG 
AATCATAAGG AAGCCAAAGA AAATTCTTTA TCGCCTTTAA AAACTTCTGA AAATTTAGAA 
GAATCCTTGC AGTTTAAAGA GAATGTGGAT ACAGAATGTA CAGAAAATTC ACCAGCACTT 
GTACCCAGTT TGAATTCTCC TAACCGATTC TGCACATGGG ACCCTGAAGA AGAACGCAAA 
CGGCAAGAAA AATGGCAGCA GGAGCAGGAA AGGCTTCTCC AGGAGAGGTA CCAGAGAGAG 
CAGGAAAAAC TTAAAGAAGA ATGGGAAAAA GCTCAGAAAG AAGTAGAAGA GGAGGAAAGG 
AAATATTATG AGGAGGAAAG AAAGATAATT GAAGACACTG TGGTCCCACT GACTTTGTCT 
CCAAATTCTA ATGCACAGTT TAATATCTCT GAAAGAAATG GGAACTTCAT TCCAGCACTT 
ACAGAACATG TCAAGAGAGA TCAAGAAAAT AACAATGAAA AGCTCTTGAC AGAGTCCAGC 
AGAACGATAA AGAATGAGAC ACATATGCAC AGCAATGTAA CACAGCAACT GCTTTTGCAT 
ACACAGGAGG GGTCAATTCT TGGTGCTAAA GAGAAACAAA TAAAGGGAAA AACAGACAAG 
TGGGAAGCAG AACAGTCCAT CTGTATTATG GACCGAGAGG AGCTGAGCAA CCATGTGCAG 
TCTAATGAAT GTAATACACC CACTAGGTTA CCCTTGCAGG CAGGATGTAA ACAAGTGACG 
TCATCAGAGT CAAGTCTGCC TGGGGATCTT GGATCACCAA AAAATCCTAT CACTGAAAAA 
GATGCTATTC AGAATATGTC GTCCATAATA CCCACACGGG GAACTGATTT CAAGGAAGAT 
ACAATGAATT CTTCACAGTC CTCTACATCA CAGTGCCAGT CACCAAACAG GTCTGTTAGT 
GGGAAAAAAC TGTGCTCCAC ATGTGGACTT CCTCTAGGCA AGGGAGCTGC CATGATAATT 
GAAACCCTCA GTCTTTATTT CCACATCCAG TGCTTCAAGT GTGGACTGTG TAAGGGACAA 
CTTGGGGATG CGATCACCGG CACAGACGTC AGAATTCGAA ATGGCCTACT GAACTGCAAT 
GACTGTTACA TGAAGTCTAG AAGTAAGGCG GCTGGCCACC CCACCCCTTT GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012811150.1
CDS	492..6185
Translation	MASSATLQGEEIPPPQESPPDTACREAQKWIEQVTGKSFGDKDFRTGLENGILLCELLNAIKPGLVKKINRLPTPIAGLDNITLFLRGCKELGLKESQLFDPGDLQDTANRTTARNSDCNRKLRNVLITVYWLGKAASSCSSYSGNNLDLKEFEGLLAQMRKENEEIESPKRSIRDSGYIDCWDSERSDSLSPPRHGRDDSFDSLDSFGSRSQQTPSPDVVLRGSSDGRGSDSESDLPHRRLPDVRKDDMSARRVSYGENKAFVPFNQYLPNKSNQTSYIPAPLRKKKAEREDYRKSWSTATSPLGGERPFSPKQSPVIKEEQNELQCEGEGTKQMIALKEPCLGAENRSERVGESEGGVMGQSSANDDIPKDAEEVRKRKHLQKAGIKVLPATKRYSSPSQFAEDIDGAPDIVLRKENTFLKSYEGMGSDSEEEEECKVPDIQRDDLATRRARMNQPKSPMQFNQYIQGSYLNKNRASRKLNSSKTSTPASPKMSQDIREYQGPQLLKPSHVCKEKTFPIPSDNKEDAEAEQEKVPDIQKDDLAKRKAKASVSLPKEPSAFAAVSITKADMETWQRLRMSSEDSEHLNDRQADQQEQTSKIDVTRATNDDFGRRKLSLQEKRARNKQKFVRFGPVTEIDQQKWDRLCIAKAESGQESEEMKGSITSDETASHISPSASAQRSQPQEDVGTGPVLPHESRSFTPERLVSPAPPENESEIQLLETQQDDMMARRTGTFQKCTRTSRDSPVSHQYALHSQQHEEKSDSLELEEKQGLFLDVNPQLRKLLLSDLANTSSKATSPTLGSKSLKVTKEAEDVKNDMKEGALNTGKDPNSVIPEDNTIAPDPEKDDMMTRKISVSQKPSCVSLGHFLPVPFSLQHREGEKKPTSCIISSTKKSKNASKAVPADISDVPSKPLDQKETKEHGELGDTTCKQASNSVETPMCTELDSQQQDGLERRMILSTDLANTELKITPPTLANNSPTITKVQEPDATISEDNTTAPNPEKDDMMTRRIYVTQKPSCVGLGHFLPVPFSLQQKVEEKKSTSSTLSIKIKSDNCTQALAVGSSDLPCKLLGQCETEGHVELRSDVTCDQTSTEIISDGIPTGKGLDTQAEDALETCDHELFRFSPRSVLSDDGESMSMCDMRYEEEGSMQPHSRAHYEHLQHVNSLLKEEDDTWQDDLAKWKTRRRSASQDLIKKEEQRKKMERMMSGEDELSERRKSIKTYREIVEEKERREKELHEAYKNAKSRDEAEHILQSYIEKFTISEAVLEGLVMPKILERSQSVEPSLLSSSEDPNILKYLRQQSLPSPKYTSTVEATVSPSSPCEAKSPTGLISPSKNIISKTVPMLTAKPYSQPKNTQEVLKTFKVDGKVNLNGVEEQKEEEHGNQTFAPSLTRSQMFEGVARVDVPVVEIKQDVTSIELNLARPNTLNSLQEPKSLCEDRNHISQEDESDSLVQLERAHSVEILSEGNTQSSNATQDVPVVSSIEIPPLTINGKDTVDERKVEAAELIMHWSLEPNHKEAKENSLSPLKTSENLEESLQFKENVDTECTENSPALVPSLNSPNRFCTWDPEEERKRQEKWQQEQERLLQERYQREQEKLKEEWEKAQKEVEEEERKYYEEERKIIEDTVVPLTLSPNSNAQFNISERNGNFIPALTEHVKRDQENNNEKLLTESSRTIKNETHMHSNVTQQLLLHTQEGSILGAKEKQIKGKTDKWEAEQSICIMDREELSNHVQSNECNTPTRLPLQAGCKQVTSSESSLPGDLGSPKNPITEKDAIQNMSSIIPTRGTDFKEDTMNSSQSSTSQCQSPNRSVSGKKLCSTCGLPLGKGAAMIIETLSLYFHIQCFKCGLCKGQLGDAITGTDVRIRNGLLNCNDCYMKSRSKAAGHPTPL

Target ORF information:

RefSeq Version	XM_012955696.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis LIM and calponin homology domains 1 (limch1), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012955696.2

ORF Insert Sequence:

ATGGCCAGCT CCGCCACACT GCAAGGGGAA GAGATCCCCC CGCCCCAGGA GTCTCCCCCA 
GACACGGCTT GCAGGGAGGC ACAGAAGTGG ATTGAGCAAG TTACTGGCAA AAGCTTTGGC 
GACAAAGACT TCAGGACTGG ACTGGAGAAT GGAATCCTTC TGTGCGAGTT ATTAAATGCT 
ATTAAACCAG GACTCGTAAA AAAGATCAAC AGACTTCCTA CTCCCATTGC TGGATTGGAC 
AATATAACAT TATTCCTTAG AGGCTGCAAA GAGCTCGGGC TGAAAGAATC TCAGTTGTTT 
GACCCAGGTG ACCTACAGGA CACAGCAAAC AGGACAACAG CCAGGAATTC AGATTGCAAC 
AGAAAACTAA GAAATGTACT AATCACCGTG TACTGGCTGG GGAAAGCTGC AAGCAGCTGC 
TCGTCCTATA GTGGCAACAA CCTGGACTTA AAGGAGTTTG AGGGATTGCT GGCACAAATG 
AGAAAAGAAA ATGAGGAAAT AGAGAGTCCA AAACGCAGCA TACGGGACAG TGGCTACATA 
GATTGTTGGG ACTCTGAGAG AAGTGACTCC CTTTCTCCTC CCAGACATGG TAGAGATGAC 
TCCTTCGATA GTTTAGACTC ATTTGGATCT AGATCACAAC AGACACCATC TCCAGATGTA 
GTGCTGAGGG GAAGCAGCGA CGGCAGAGGA AGCGACTCTG AGTCTGACCT ACCTCACAGA 
AGGTTACCTG ATGTGCGAAA AGATGATATG TCTGCTAGAC GGGTATCCTA CGGTGAAAAC 
AAAGCATTTG TGCCTTTCAA CCAATACCTC CCCAACAAGA GCAACCAGAC CTCCTACATC 
CCGGCACCCC TGAGGAAGAA GAAGGCTGAG CGTGAGGACT ACCGGAAAAG CTGGAGCACA 
GCTACATCCC CCCTGGGGGG AGAGCGACCC TTCAGTCCCA AACAAAGTCC AGTCATTAAA 
GAAGAACAAA ACGAGTTGCA GTGTGAAGGA GAAGGGACCA AGCAGATGAT TGCCTTAAAA 
GAACCTTGCC TTGGAGCAGA AAATAGGAGT GAGAGAGTTG GTGAAAGTGA AGGGGGTGTC 
ATGGGACAAT CATCTGCTAA TGATGACATT CCAAAAGATG CAGAGGAAGT GAGAAAGCGC 
AAGCACCTTC AGAAAGCGGG TATCAAGGTC TTGCCCGCAA CAAAGCGCTA CAGCAGTCCA 
AGTCAATTTG CTGAAGACAT TGATGGCGCT CCAGACATTG TGCTGCGAAA AGAGAACACC 
TTCCTGAAGT CATATGAAGG GATGGGCTCT GATTCCGAAG AAGAAGAAGA GTGCAAAGTA 
CCTGATATAC AGCGAGATGA CTTGGCCACA AGGCGTGCAA GGATGAACCA ACCAAAAAGC 
CCAATGCAGT TTAATCAGTA TATACAGGGG TCATACTTAA ACAAAAACAG AGCATCAAGA 
AAGCTGAACA GTTCTAAGAC TTCCACTCCA GCTTCTCCAA AAATGTCTCA AGATATCAGG 
GAGTACCAAG GCCCCCAGTT ATTAAAGCCA TCACACGTCT GCAAAGAAAA GACATTTCCA 
ATACCCTCAG ACAATAAAGA GGATGCAGAA GCAGAGCAAG AAAAAGTTCC CGACATTCAG 
AAAGACGACC TGGCGAAACG CAAAGCTAAA GCTTCAGTTT CACTGCCAAA GGAACCATCG 
GCCTTTGCAG CGGTTTCAAT TACAAAGGCC GACATGGAAA CTTGGCAGAG GCTTCGAATG 
TCTTCTGAAG ATAGTGAGCA CTTGAATGAC AGGCAGGCTG ACCAGCAAGA GCAAACATCC 
AAGATTGATG TGACTAGAGC GACAAATGAT GACTTTGGGA GGAGGAAATT GTCTCTGCAA 
GAGAAAAGGG CTCGAAACAA ACAGAAGTTT GTACGCTTTG GCCCAGTGAC TGAAATTGAT 
CAGCAGAAGT GGGATAGGCT TTGTATAGCC AAGGCTGAAT CTGGCCAGGA AAGTGAAGAG 
ATGAAGGGCA GCATTACGTC TGATGAAACT GCCAGCCACA TATCTCCCTC AGCTTCTGCT 
CAGCGCTCCC AACCCCAGGA GGATGTTGGC ACAGGGCCAG TCTTGCCACA TGAGTCAAGA 
TCTTTTACAC CAGAGAGATT GGTTTCACCT GCTCCTCCTG AAAATGAAAG TGAAATTCAG 
CTCCTAGAAA CACAGCAAGA TGACATGATG GCAAGGAGAA CAGGTACATT CCAAAAATGC 
ACCAGAACCA GCAGAGACTC TCCTGTGTCC CACCAATATG CACTCCATAG CCAACAACAC 
GAAGAGAAGT CTGACTCGCT GGAACTCGAA GAGAAACAAG GACTCTTTCT GGATGTTAAC 
CCACAACTGA GAAAATTACT GCTGTCGGAC TTGGCAAATA CTAGTTCAAA AGCAACTAGC 
CCTACTTTGG GGAGCAAGTC TCTGAAAGTC ACCAAAGAAG CTGAAGATGT AAAAAATGAC 
ATGAAAGAAG GTGCCTTGAA CACAGGAAAG GATCCAAACT CTGTTATCCC AGAGGATAAC 
ACAATTGCAC CAGACCCAGA AAAAGATGAC ATGATGACAA GAAAAATCTC AGTGAGCCAG 
AAGCCATCTT GCGTCAGCCT TGGACACTTC CTTCCTGTTC CTTTTTCTTT GCAGCACAGA 
GAGGGGGAAA AGAAACCAAC AAGCTGCATC ATATCAAGCA CCAAAAAGTC CAAAAATGCA 
TCCAAAGCTG TTCCAGCCGA CATTTCTGAT GTTCCTTCTA AGCCCCTTGA CCAGAAAGAA 
ACAAAAGAAC ATGGTGAATT AGGAGATACG ACTTGCAAGC AAGCCTCCAA TAGTGTGGAG 
ACTCCCATGT GTACAGAGTT AGACAGCCAG CAACAGGATG GTCTTGAGAG GAGAATGATA 
CTGTCTACTG ACTTGGCCAA TACTGAGTTA AAAATAACTC CTCCTACTTT GGCCAACAAC 
TCTCCAACCA TAACAAAAGT ACAAGAGCCA GATGCAACCA TCTCAGAGGA CAATACAACT 
GCACCAAACC CTGAAAAAGA TGATATGATG ACCAGAAGAA TCTATGTAAC CCAGAAACCA 
TCGTGTGTAG GTCTTGGCCA CTTCCTTCCA GTTCCATTTT CTTTGCAGCA AAAAGTTGAG 
GAAAAGAAAT CAACGAGCTC CACTCTATCG ATCAAAATAA AGTCAGATAA TTGCACCCAA 
GCACTTGCAG TCGGCAGTTC TGACCTTCCT TGTAAGCTCC TCGGCCAATG TGAAACAGAG 
GGACATGTTG AATTACGATC CGATGTGACT TGCGATCAAA CCTCCACTGA GATCATTAGT 
GATGGGATTC CCACTGGTAA AGGGTTAGAT ACCCAGGCAG AAGACGCTCT TGAGACATGT 
GACCATGAGT TATTCAGATT CAGCCCAAGA TCTGTTCTGT CTGATGATGG AGAGAGCATG 
AGCATGTGCG ACATGAGGTA CGAAGAAGAG GGGAGTATGC AGCCTCACAG CAGGGCACAC 
TATGAGCATC TACAGCATGT CAACAGCTTG CTCAAGGAGG AGGATGACAC ATGGCAAGAT 
GACCTAGCTA AGTGGAAGAC ACGGAGAAGA AGTGCATCAC AGGATCTGAT CAAAAAAGAG 
GAACAGAGGA AGAAGATGGA GAGAATGATG AGCGGAGAGG ATGAACTGTC GGAGCGTAGG 
AAAAGCATAA AAACATATAG AGAGATTGTG GAAGAAAAAG AACGTCGAGA GAAAGAACTG 
CATGAAGCCT ACAAAAATGC CAAGTCAAGA GATGAGGCTG AACACATATT ACAGAGCTAC 
ATTGAAAAGT TTACAATTAG TGAAGCTGTT CTTGAGGGAT TAGTAATGCC AAAAATCTTA 
GAAAGAAGCC AGTCGGTGGA GCCAAGTTTA TTGTCTTCAT CAGAGGACCC AAACATCCTG 
AAATACCTGA GGCAACAGTC TTTACCATCA CCAAAATACA CTTCCACTGT TGAAGCAACT 
GTCTCCCCCA GCAGCCCATG TGAAGCAAAA AGTCCAACAG GTCTGATATC ACCAAGCAAA 
AACATTATCT CAAAGACTGT GCCTATGCTA ACAGCCAAGC CTTACTCCCA GCCCAAAAAC 
ACCCAGGAAG TCCTCAAGAC ATTTAAGGTA GATGGAAAGG TAAACCTCAA CGGGGTAGAA 
GAGCAGAAAG AAGAGGAACA TGGAAACCAG ACATTTGCCC CATCACTCAC CCGATCTCAG 
ATGTTTGAAG GTGTAGCCAG AGTTGATGTT CCTGTTGTTG AAATAAAGCA AGACGTAACT 
TCCATAGAGC TTAATCTGGC CAGACCAAAC ACCCTGAATT CCTTACAAGA ACCCAAAAGT 
CTCTGCGAAG ACAGAAATCA CATAAGCCAG GAGGATGAGT CAGATTCTCT GGTGCAATTA 
GAAAGAGCTC ACTCCGTGGA AATATTGTCA GAAGGTAACA CCCAATCAAG CAATGCAACC 
CAGGATGTAC CTGTTGTGAG CAGTATAGAG ATTCCACCAT TGACCATCAA TGGAAAGGAC 
ACTGTGGATG AGAGGAAAGT GGAAGCTGCC GAGTTGATCA TGCATTGGTC CCTGGAGCCG 
AATCATAAGG AAGCCAAAGA AAATTCTTTA TCGCCTTTAA AAACTTCTGA AAATTTAGAA 
GAATCCTTGC AGTTTAAAGA GAATGTGGAT ACAGAATGTA CAGAAAATTC ACCAGCACTT 
GTACCCAGTT TGAATTCTCC TAACCGATTC TGCACATGGG ACCCTGAAGA AGAACGCAAA 
CGGCAAGAAA AATGGCAGCA GGAGCAGGAA AGGCTTCTCC AGGAGAGGTA CCAGAGAGAG 
CAGGAAAAAC TTAAAGAAGA ATGGGAAAAA GCTCAGAAAG AAGTAGAAGA GGAGGAAAGG 
AAATATTATG AGGAGGAAAG AAAGATAATT GAAGACACTG TGGTCCCACT GACTTTGTCT 
CCAAATTCTA ATGCACAGTT TAATATCTCT GAAAGAAATG GGAACTTCAT TCCAGCACTT 
ACAGAACATG TCAAGAGAGA TCAAGAAAAT AACAATGAAA AGCTCTTGAC AGAGTCCAGC 
AGAACGATAA AGAATGAGAC ACATATGCAC AGCAATGTAA CACAGCAACT GCTTTTGCAT 
ACACAGGAGG GGTCAATTCT TGGTGCTAAA GAGAAACAAA TAAAGGGAAA AACAGACAAG 
TGGGAAGCAG AACAGTCCAT CTGTATTATG GACCGAGAGG AGCTGAGCAA CCATGTGCAG 
TCTAATGAAT GTAATACACC CACTAGGTTA CCCTTGCAGG CAGGATGTAA ACAAGTGACG 
TCATCAGAGT CAAGTCTGCC TGGGGATCTT GGATCACCAA AAAATCCTAT CACTGAAAAA 
GATGCTATTC AGAATATGTC GTCCATAATA CCCACACGGG GAACTGATTT CAAGGAAGAT 
ACAATGAATT CTTCACAGTC CTCTACATCA CAGTGCCAGT CACCAAACAG GTCTGTTAGT 
GGGAAAAAAC TGTGCTCCAC ATGTGGACTT CCTCTAGGCA AGGGAGCTGC CATGATAATT 
GAAACCCTCA GTCTTTATTT CCACATCCAG TGCTTCAAGT GTGGACTGTG TAAGGGACAA 
CTTGGGGATG CGATCACCGG CACAGACGTC AGAATTCGAA ATGGCCTACT GAACTGCAAT 
GACTGTTACA TGAAGTCTAG AAGTAAGGCG GCTGGCCACC CCACCCCTTT GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

limch1 ( XM_012955698.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012811152.1 Xenopus tropicalis LIM and calponin homology domains 1 (limch1), transcript variant X5, mRNA.

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CloneID	OXa30869
Clone ID Related Accession (Same CDS sequence)	XM_012955698.2
Accession Version	XM_012955698.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5682bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1473177600000
Organism	Xenopus tropicalis(tropical clawed frog)
Product	LIM and calponin homology domains-containing protein 1 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012955698.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCAGCT CCGCCACACT GCAAGGGGAA GAGATCCCCC CGCCCCAGGA GTCTCCCCCA 
GACACGGCTT GCAGGGAGGC ACAGAAGTGG ATTGAGCAAG TTACTGGCAA AAGCTTTGGC 
GACAAAGACT TCAGGACTGG ACTGGAGAAT GGAATCCTTC TGTGCGAGTT ATTAAATGCT 
ATTAAACCAG GACTCGTAAA AAAGATCAAC AGACTTCCTA CTCCCATTGC TGGATTGGAC 
AATATAACAT TATTCCTTAG AGGCTGCAAA GAGCTCGGGC TGAAAGAATC TCAGTTGTTT 
GACCCAGGTG ACCTACAGGA CACAGCAAAC AGGACAACAG CCAGGAATTC AGATTGCAAC 
AGAAAACTAA GAAATGTACT AATCACCGTG TACTGGCTGG GGAAAGCTGC AAGCAGCTGC 
TCGTCCTATA GTGGCAACAA CCTGGACTTA AAGGAGTTTG AGGGATTGCT GGCACAAATG 
AGAAAAGAAA ATGAGGAAAT AGAGAGTCCA AAACGCAGCA TACGGGACAG TGGCTACATA 
GATTGTTGGG ACTCTGAGAG AAGTGACTCC CTTTCTCCTC CCAGACATGG TAGAGATGAC 
TCCTTCGATA GTTTAGACTC ATTTGGATCT AGATCACAAC AGACACCATC TCCAGATGTA 
GTGCTGAGGG GAAGCAGCGA CGGCAGAGGA AGCGACTCTG AGTCTGACCT ACCTCACAGA 
AGGTTACCTG ATGTGCGAAA AGATGATATG TCTGCTAGAC GGGTATCCTA CGGTGAAAAC 
AAAGCATTTG TGCCTTTCAA CCAATACCTC CCCAACAAGA GCAACCAGAC CTCCTACATC 
CCGGCACCCC TGAGGAAGAA GAAGGCTGAG CGTGAGGACT ACCGGAAAAG CTGGAGCACA 
GCTACATCCC CCCTGGGGGG AGAGCGACCC TTCAGTCCCA AACAAAGTCC AGTCATTAAA 
GAAGAACAAA ACGAGTTGCA GTGTGAAGGA GAAGGGACCA AGCAGATGAT TGCCTTAAAA 
GAACCTTGCC TTGGAGCAGA AAATAGGAGT GAGAGAGTTG GTGAAAGTGA AGGGGGTGTC 
ATGGGACAAT CATCTGCTAA TGATGACATT CCAAAAGATG CAGAGGAAGT GAGAAAGCGC 
AAGCACCTTC AGAAAGCGGG TATCAAGGTC TTGCCCGCAA CAAAGCGCTA CAGCAGTCCA 
AGTCAATTTG CTGAAGACAT TGATGGCGCT CCAGACATTG TGCTGCGAAA AGAGAACACC 
TTCCTGAAGT CATATGAAGG GATGGGCTCT GATTCCGAAG AAGAAGAAGA GTGCAAAGTA 
CCTGATATAC AGCGAGATGA CTTGGCCACA AGGCGTGCAA GGATGAACCA ACCAAAAAGC 
CCAATGCAGT TTAATCAGTA TATACAGGGG TCATACTTAA ACAAAAACAG AGCATCAAGA 
AAGCTGAACA GTTCTAAGAC TTCCACTCCA GCTTCTCCAA AAATGTCTCA AGATATCAGG 
GAGTACCAAG GCCCCCAGTT ATTAAAGCCA TCACACGTCT GCAAAGAAAA GACATTTCCA 
ATACCCTCAG ACAATAAAGA GGATGCAGAA GCAGAGCAAG AAAAAGTTCC CGACATTCAG 
AAAGACGACC TGGCGAAACG CAAAGCTAAA GCTTCAGTTT CACTGCCAAA GGAACCATCG 
GCCTTTGCAG CGGTTTCAAT TACAAAGGCC GACATGGAAA CTTGGCAGAG GCTTCGAATG 
TCTTCTGAAG ATAGTGAGCA CTTGAATGAC AGGCAGGCTG ACCAGCAAGA GCAAACATCC 
AAGATTGATG TGACTAGAGC GACAAATGAT GACTTTGGGA GGAGGAAATT GTCTCTGCAA 
GAGAAAAGGG CTCGAAACAA ACAGAAGTTT GTACGCTTTG GCCCAGTGAC TGAAATTGAT 
CAGCAGAAGT GGGATAGGCT TTGTATAGCC AAGGCTGAAT CTGGCCAGGA AAGTGAAGAG 
ATGAAGGGCA GCATTACGTC TGATGAAACT GCCAGCCACA TATCTCCCTC AGCTTCTGCT 
CAGCGCTCCC AACCCCAGGA GGATGTTGGC ACAGGGCCAG TCTTGCCACA TGAGTCAAGA 
TCTTTTACAC CAGAGAGATT GGTTTCACCT GCTCCTCCTG AAAATGAAAG TGAAATTCAG 
CTCCTAGAAA CACAGCAAGA TGACATGATG GCAAGGAGAA CAGGTACATT CCAAAAATGC 
ACCAGAACCA GCAGAGACTC TCCTGTGTCC CACCAATATG CACTCCATAG CCAACAACAC 
GAAGAGAAGT CTGACTCGCT GGAACTCGAA GAGAAACAAG GACTCTTTCT GGATGTTAAC 
CCACAACTGA GAAAATTACT GCTGTCGGAC TTGGCAAATA CTAGTTCAAA AGCAACTAGC 
CCTACTTTGG GGAGCAAGTC TCTGAAAGTC ACCAAAGAAG CTGAAGATGT AAAAAATGAC 
ATGAAAGAAG GTGCCTTGAA CACAGGAAAG GATCCAAACT CTGTTATCCC AGAGGATAAC 
ACAATTGCAC CAGACCCAGA AAAAGATGAC ATGATGACAA GAAAAATCTC AGTGAGCCAG 
AAGCCATCTT GCGTCAGCCT TGGACACTTC CTTCCTGTTC CTTTTTCTTT GCAGCACAGA 
GAGGGGGAAA AGAAACCAAC AAGCTGCATC ATATCAAGCA CCAAAAAGTC CAAAAATGCA 
TCCAAAGCTG TTCCAGCCGA CATTTCTGAT GTTCCTTCTA AGCCCCTTGA CCAGAAAGAA 
ACAAAAGAAC ATGGTGAATT AGGAGATACG ACTTGCAAGC AAGCCTCCAA TAGTGTGGAG 
ACTCCCATGT GTACAGAGTT AGACAGCCAG CAACAGGATG GTCTTGAGAG GAGAATGATA 
CTGTCTACTG ACTTGGCCAA TACTGAGTTA AAAATAACTC CTCCTACTTT GGCCAACAAC 
TCTCCAACCA TAACAAAAGT ACAAGAGCCA GATGCAACCA TCTCAGAGGA CAATACAACT 
GCACCAAACC CTGAAAAAGA TGATATGATG ACCAGAAGAA TCTATGTAAC CCAGAAACCA 
TCGTGTGTAG GTCTTGGCCA CTTCCTTCCA GTTCCATTTT CTTTGCAGCA AAAAGTTGAG 
GAAAAGAAAT CAACGAGCTC CACTCTATCG ATCAAAATAA AGTCAGATAA TTGCACCCAA 
GCACTTGCAG TCGGCAGTTC TGACCTTCCT TGTAAGCTCC TCGGCCAATG TGAAACAGAG 
GGACATGTTG AATTACGATC CGATGTGACT TGCGATCAAA CCTCCACTGA GATCATTAGT 
GATGGGATTC CCACTGGTAA AGGGTTAGAT ACCCAGGCAG AAGACGCTCT TGAGACATGT 
GACCATGAGT TATTCAGATT CAGCCCAAGA TCTGTTCTGT CTGATGATGG AGAGAGCATG 
AGCATGTGCG ACATGAGGTA CGAAGAAGAG GGGAGTATGC AGCCTCACAG CAGGGCACAC 
TATGAGCATC TACAGCATGT CAACAGCTTG CTCAAGGAGG AGGATGACAC ATGGCAAGAT 
GACCTAGCTA AGTGGAAGAC ACGGAGAAGA AGTGCATCAC AGGATCTGAT CAAAAAAGAG 
GAACAGAGGA AGAAGATGGA GAGAATGATG AGCGGAGAGG ATGAACTGTC GGAGCGTAGG 
AAAAGCATAA AAACATATAG AGAGATTGTG GAAGAAAAAG AACGTCGAGA GAAAGAACTG 
CATGAAGCCT ACAAAAATGC CAAGTCAAGA GATGAGGCTG AACACATATT ACAGAGCTAC 
ATTGAAAAGT TTACAATTAG TGAAGCTGTT CTTGAGGGAT TAGTAATGCC AAAAATCTTA 
GAAAGAAGCC AGTCGGTGGA GCCAAGTTTA TTGTCTTCAT CAGAGGACCC AAACATCCTG 
AAATACCTGA GGCAACAGTC TTTACCATCA CCAAAATACA CTTCCACTGT TGAAGCAACT 
GTCTCCCCCA GCAGCCCATG TGAAGCAAAA AGTCCAACAG GTCTGATATC ACCAAGCAAA 
AACATTATCT CAAAGACTGT GCCTATGCTA ACAGCCAAGC CTTACTCCCA GCCCAAAAAC 
ACCCAGGAAG TCCTCAAGAC ATTTAAGGTA GATGGAAAGG TAAACCTCAA CGGGGTAGAA 
GAGCAGAAAG AAGAGGAACA TGGAAACCAG ACATTTGCCC CATCACTCAC CCGATCTCAG 
ATGTTTGAAG GTGTAGCCAG AGTTGATGTT CCTGTTGTTG AAATAAAGCA AGACGTAACT 
TCCATAGAGC TTAATCTGGC CAGACCAAAC ACCCTGAATT CCTTACAAGA ACCCAAAAGT 
CTCTGCGAAG ACAGAAATCA CATAAGCCAG GAGGATGAGT CAGATTCTCT GGTGCAATTA 
GAAAGAGCTC ACTCCGTGGA AATATTGTCA GAAGGTAACA CCCAATCAAG CAATGCAACC 
CAGGATGTAC CTGTTGTGAG CAGTATAGAG ATTCCACCAT TGACCATCAA TGGAAAGGAC 
ACTGTGGATG AGAGGAAAGT GGAAGCTGCC GAGTTGATCA TGCATTGGTC CCTGGAGCCG 
AATCATAAGG AAGCCAAAGA AAATTCTTTA TCGCCTTTAA AAACTTCTGA AAATTTAGAA 
GAATCCTTGC AGTTTAAAGA GAATGAATCA TCACACAAAG TGCAGCTAAA CATTACGCTT 
CAGAGCCGGA ACGCCTGGAG ACGCTCCCAG TTTTTTTTGC AGCCAGTGGA TACAGAATGT 
ACAGAAAATT CACCAGCACT TGTACCCAGT TTGAATTCTC CTAACCGATT CTGCACATGG 
GACCCTGAAG AAGAACGCAA ACGGCAAGAA AAATGGCAGC AGGAGCAGGA AAGGCTTCTC 
CAGGAAAGAA AGATAATTGA AGACACTGTG GTCCCACTGA CTTTGTCTCC AAATTCTAAT 
GCACAGTTTA ATATCTCTGA AAGAAATGGG AACTTCATTC CAGCACTTAC AGAACATGTC 
AAGAGAGATC AAGAAAATAA CAATGAAAAG CTCTTGACAG AGTCCAGCAG AACGATAAAG 
AATGAGACAC ATATGCACAG CAATGTAACA CAGCAACTGC TTTTGCATAC ACAGGAGGGG 
TCAATTCTTG GTGCTAAAGA GAAACAAATA AAGGGAAAAA CAGACAAGTG GGAAGCAGAA 
CAGTCCATCT GTATTATGGA CCGAGAGGAG CTGAGCAACC ATGTGCAGTC TAATGAATGT 
AATACACCCA CTAGGTTACC CTTGCAGGCA GGATGTAAAC AAGTGACGTC ATCAGAGTCA 
AGTCTGCCTG GGGATCTTGG ATCACCAAAA AATCCTATCA CTGAAAAAGA TGCTATTCAG 
AATATGTCGT CCATAATACC CACACGGGGA ACTGATTTCA AGGAAGATAC AATGAATTCT 
TCACAGTCCT CTACATCACA GTGCCAGTCA CCAAACAGGT CTGTTAGTGG GAAAAAACTG 
TGCTCCACAT GTGGACTTCC TCTAGGCAAG GGAGCTGCCA TGATAATTGA AACCCTCAGT 
CTTTATTTCC ACATCCAGTG CTTCAAGTGT GGACTGTGTA AGGGACAACT TGGGGATGCG 
ATCACCGGCA CAGACGTCAG AATTCGAAAT GGCCTACTGA ACTGCAATGA CTGTTACATG 
AAGTCTAGAA GTAAGGCGGC TGGCCACCCC ACCCCTTTGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012811152.1
CDS	492..6173
Translation	MASSATLQGEEIPPPQESPPDTACREAQKWIEQVTGKSFGDKDFRTGLENGILLCELLNAIKPGLVKKINRLPTPIAGLDNITLFLRGCKELGLKESQLFDPGDLQDTANRTTARNSDCNRKLRNVLITVYWLGKAASSCSSYSGNNLDLKEFEGLLAQMRKENEEIESPKRSIRDSGYIDCWDSERSDSLSPPRHGRDDSFDSLDSFGSRSQQTPSPDVVLRGSSDGRGSDSESDLPHRRLPDVRKDDMSARRVSYGENKAFVPFNQYLPNKSNQTSYIPAPLRKKKAEREDYRKSWSTATSPLGGERPFSPKQSPVIKEEQNELQCEGEGTKQMIALKEPCLGAENRSERVGESEGGVMGQSSANDDIPKDAEEVRKRKHLQKAGIKVLPATKRYSSPSQFAEDIDGAPDIVLRKENTFLKSYEGMGSDSEEEEECKVPDIQRDDLATRRARMNQPKSPMQFNQYIQGSYLNKNRASRKLNSSKTSTPASPKMSQDIREYQGPQLLKPSHVCKEKTFPIPSDNKEDAEAEQEKVPDIQKDDLAKRKAKASVSLPKEPSAFAAVSITKADMETWQRLRMSSEDSEHLNDRQADQQEQTSKIDVTRATNDDFGRRKLSLQEKRARNKQKFVRFGPVTEIDQQKWDRLCIAKAESGQESEEMKGSITSDETASHISPSASAQRSQPQEDVGTGPVLPHESRSFTPERLVSPAPPENESEIQLLETQQDDMMARRTGTFQKCTRTSRDSPVSHQYALHSQQHEEKSDSLELEEKQGLFLDVNPQLRKLLLSDLANTSSKATSPTLGSKSLKVTKEAEDVKNDMKEGALNTGKDPNSVIPEDNTIAPDPEKDDMMTRKISVSQKPSCVSLGHFLPVPFSLQHREGEKKPTSCIISSTKKSKNASKAVPADISDVPSKPLDQKETKEHGELGDTTCKQASNSVETPMCTELDSQQQDGLERRMILSTDLANTELKITPPTLANNSPTITKVQEPDATISEDNTTAPNPEKDDMMTRRIYVTQKPSCVGLGHFLPVPFSLQQKVEEKKSTSSTLSIKIKSDNCTQALAVGSSDLPCKLLGQCETEGHVELRSDVTCDQTSTEIISDGIPTGKGLDTQAEDALETCDHELFRFSPRSVLSDDGESMSMCDMRYEEEGSMQPHSRAHYEHLQHVNSLLKEEDDTWQDDLAKWKTRRRSASQDLIKKEEQRKKMERMMSGEDELSERRKSIKTYREIVEEKERREKELHEAYKNAKSRDEAEHILQSYIEKFTISEAVLEGLVMPKILERSQSVEPSLLSSSEDPNILKYLRQQSLPSPKYTSTVEATVSPSSPCEAKSPTGLISPSKNIISKTVPMLTAKPYSQPKNTQEVLKTFKVDGKVNLNGVEEQKEEEHGNQTFAPSLTRSQMFEGVARVDVPVVEIKQDVTSIELNLARPNTLNSLQEPKSLCEDRNHISQEDESDSLVQLERAHSVEILSEGNTQSSNATQDVPVVSSIEIPPLTINGKDTVDERKVEAAELIMHWSLEPNHKEAKENSLSPLKTSENLEESLQFKENESSHKVQLNITLQSRNAWRRSQFFLQPVDTECTENSPALVPSLNSPNRFCTWDPEEERKRQEKWQQEQERLLQERKIIEDTVVPLTLSPNSNAQFNISERNGNFIPALTEHVKRDQENNNEKLLTESSRTIKNETHMHSNVTQQLLLHTQEGSILGAKEKQIKGKTDKWEAEQSICIMDREELSNHVQSNECNTPTRLPLQAGCKQVTSSESSLPGDLGSPKNPITEKDAIQNMSSIIPTRGTDFKEDTMNSSQSSTSQCQSPNRSVSGKKLCSTCGLPLGKGAAMIIETLSLYFHIQCFKCGLCKGQLGDAITGTDVRIRNGLLNCNDCYMKSRSKAAGHPTPL

Target ORF information:

RefSeq Version	XM_012955698.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis LIM and calponin homology domains 1 (limch1), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012955698.2

ORF Insert Sequence:

ATGGCCAGCT CCGCCACACT GCAAGGGGAA GAGATCCCCC CGCCCCAGGA GTCTCCCCCA 
GACACGGCTT GCAGGGAGGC ACAGAAGTGG ATTGAGCAAG TTACTGGCAA AAGCTTTGGC 
GACAAAGACT TCAGGACTGG ACTGGAGAAT GGAATCCTTC TGTGCGAGTT ATTAAATGCT 
ATTAAACCAG GACTCGTAAA AAAGATCAAC AGACTTCCTA CTCCCATTGC TGGATTGGAC 
AATATAACAT TATTCCTTAG AGGCTGCAAA GAGCTCGGGC TGAAAGAATC TCAGTTGTTT 
GACCCAGGTG ACCTACAGGA CACAGCAAAC AGGACAACAG CCAGGAATTC AGATTGCAAC 
AGAAAACTAA GAAATGTACT AATCACCGTG TACTGGCTGG GGAAAGCTGC AAGCAGCTGC 
TCGTCCTATA GTGGCAACAA CCTGGACTTA AAGGAGTTTG AGGGATTGCT GGCACAAATG 
AGAAAAGAAA ATGAGGAAAT AGAGAGTCCA AAACGCAGCA TACGGGACAG TGGCTACATA 
GATTGTTGGG ACTCTGAGAG AAGTGACTCC CTTTCTCCTC CCAGACATGG TAGAGATGAC 
TCCTTCGATA GTTTAGACTC ATTTGGATCT AGATCACAAC AGACACCATC TCCAGATGTA 
GTGCTGAGGG GAAGCAGCGA CGGCAGAGGA AGCGACTCTG AGTCTGACCT ACCTCACAGA 
AGGTTACCTG ATGTGCGAAA AGATGATATG TCTGCTAGAC GGGTATCCTA CGGTGAAAAC 
AAAGCATTTG TGCCTTTCAA CCAATACCTC CCCAACAAGA GCAACCAGAC CTCCTACATC 
CCGGCACCCC TGAGGAAGAA GAAGGCTGAG CGTGAGGACT ACCGGAAAAG CTGGAGCACA 
GCTACATCCC CCCTGGGGGG AGAGCGACCC TTCAGTCCCA AACAAAGTCC AGTCATTAAA 
GAAGAACAAA ACGAGTTGCA GTGTGAAGGA GAAGGGACCA AGCAGATGAT TGCCTTAAAA 
GAACCTTGCC TTGGAGCAGA AAATAGGAGT GAGAGAGTTG GTGAAAGTGA AGGGGGTGTC 
ATGGGACAAT CATCTGCTAA TGATGACATT CCAAAAGATG CAGAGGAAGT GAGAAAGCGC 
AAGCACCTTC AGAAAGCGGG TATCAAGGTC TTGCCCGCAA CAAAGCGCTA CAGCAGTCCA 
AGTCAATTTG CTGAAGACAT TGATGGCGCT CCAGACATTG TGCTGCGAAA AGAGAACACC 
TTCCTGAAGT CATATGAAGG GATGGGCTCT GATTCCGAAG AAGAAGAAGA GTGCAAAGTA 
CCTGATATAC AGCGAGATGA CTTGGCCACA AGGCGTGCAA GGATGAACCA ACCAAAAAGC 
CCAATGCAGT TTAATCAGTA TATACAGGGG TCATACTTAA ACAAAAACAG AGCATCAAGA 
AAGCTGAACA GTTCTAAGAC TTCCACTCCA GCTTCTCCAA AAATGTCTCA AGATATCAGG 
GAGTACCAAG GCCCCCAGTT ATTAAAGCCA TCACACGTCT GCAAAGAAAA GACATTTCCA 
ATACCCTCAG ACAATAAAGA GGATGCAGAA GCAGAGCAAG AAAAAGTTCC CGACATTCAG 
AAAGACGACC TGGCGAAACG CAAAGCTAAA GCTTCAGTTT CACTGCCAAA GGAACCATCG 
GCCTTTGCAG CGGTTTCAAT TACAAAGGCC GACATGGAAA CTTGGCAGAG GCTTCGAATG 
TCTTCTGAAG ATAGTGAGCA CTTGAATGAC AGGCAGGCTG ACCAGCAAGA GCAAACATCC 
AAGATTGATG TGACTAGAGC GACAAATGAT GACTTTGGGA GGAGGAAATT GTCTCTGCAA 
GAGAAAAGGG CTCGAAACAA ACAGAAGTTT GTACGCTTTG GCCCAGTGAC TGAAATTGAT 
CAGCAGAAGT GGGATAGGCT TTGTATAGCC AAGGCTGAAT CTGGCCAGGA AAGTGAAGAG 
ATGAAGGGCA GCATTACGTC TGATGAAACT GCCAGCCACA TATCTCCCTC AGCTTCTGCT 
CAGCGCTCCC AACCCCAGGA GGATGTTGGC ACAGGGCCAG TCTTGCCACA TGAGTCAAGA 
TCTTTTACAC CAGAGAGATT GGTTTCACCT GCTCCTCCTG AAAATGAAAG TGAAATTCAG 
CTCCTAGAAA CACAGCAAGA TGACATGATG GCAAGGAGAA CAGGTACATT CCAAAAATGC 
ACCAGAACCA GCAGAGACTC TCCTGTGTCC CACCAATATG CACTCCATAG CCAACAACAC 
GAAGAGAAGT CTGACTCGCT GGAACTCGAA GAGAAACAAG GACTCTTTCT GGATGTTAAC 
CCACAACTGA GAAAATTACT GCTGTCGGAC TTGGCAAATA CTAGTTCAAA AGCAACTAGC 
CCTACTTTGG GGAGCAAGTC TCTGAAAGTC ACCAAAGAAG CTGAAGATGT AAAAAATGAC 
ATGAAAGAAG GTGCCTTGAA CACAGGAAAG GATCCAAACT CTGTTATCCC AGAGGATAAC 
ACAATTGCAC CAGACCCAGA AAAAGATGAC ATGATGACAA GAAAAATCTC AGTGAGCCAG 
AAGCCATCTT GCGTCAGCCT TGGACACTTC CTTCCTGTTC CTTTTTCTTT GCAGCACAGA 
GAGGGGGAAA AGAAACCAAC AAGCTGCATC ATATCAAGCA CCAAAAAGTC CAAAAATGCA 
TCCAAAGCTG TTCCAGCCGA CATTTCTGAT GTTCCTTCTA AGCCCCTTGA CCAGAAAGAA 
ACAAAAGAAC ATGGTGAATT AGGAGATACG ACTTGCAAGC AAGCCTCCAA TAGTGTGGAG 
ACTCCCATGT GTACAGAGTT AGACAGCCAG CAACAGGATG GTCTTGAGAG GAGAATGATA 
CTGTCTACTG ACTTGGCCAA TACTGAGTTA AAAATAACTC CTCCTACTTT GGCCAACAAC 
TCTCCAACCA TAACAAAAGT ACAAGAGCCA GATGCAACCA TCTCAGAGGA CAATACAACT 
GCACCAAACC CTGAAAAAGA TGATATGATG ACCAGAAGAA TCTATGTAAC CCAGAAACCA 
TCGTGTGTAG GTCTTGGCCA CTTCCTTCCA GTTCCATTTT CTTTGCAGCA AAAAGTTGAG 
GAAAAGAAAT CAACGAGCTC CACTCTATCG ATCAAAATAA AGTCAGATAA TTGCACCCAA 
GCACTTGCAG TCGGCAGTTC TGACCTTCCT TGTAAGCTCC TCGGCCAATG TGAAACAGAG 
GGACATGTTG AATTACGATC CGATGTGACT TGCGATCAAA CCTCCACTGA GATCATTAGT 
GATGGGATTC CCACTGGTAA AGGGTTAGAT ACCCAGGCAG AAGACGCTCT TGAGACATGT 
GACCATGAGT TATTCAGATT CAGCCCAAGA TCTGTTCTGT CTGATGATGG AGAGAGCATG 
AGCATGTGCG ACATGAGGTA CGAAGAAGAG GGGAGTATGC AGCCTCACAG CAGGGCACAC 
TATGAGCATC TACAGCATGT CAACAGCTTG CTCAAGGAGG AGGATGACAC ATGGCAAGAT 
GACCTAGCTA AGTGGAAGAC ACGGAGAAGA AGTGCATCAC AGGATCTGAT CAAAAAAGAG 
GAACAGAGGA AGAAGATGGA GAGAATGATG AGCGGAGAGG ATGAACTGTC GGAGCGTAGG 
AAAAGCATAA AAACATATAG AGAGATTGTG GAAGAAAAAG AACGTCGAGA GAAAGAACTG 
CATGAAGCCT ACAAAAATGC CAAGTCAAGA GATGAGGCTG AACACATATT ACAGAGCTAC 
ATTGAAAAGT TTACAATTAG TGAAGCTGTT CTTGAGGGAT TAGTAATGCC AAAAATCTTA 
GAAAGAAGCC AGTCGGTGGA GCCAAGTTTA TTGTCTTCAT CAGAGGACCC AAACATCCTG 
AAATACCTGA GGCAACAGTC TTTACCATCA CCAAAATACA CTTCCACTGT TGAAGCAACT 
GTCTCCCCCA GCAGCCCATG TGAAGCAAAA AGTCCAACAG GTCTGATATC ACCAAGCAAA 
AACATTATCT CAAAGACTGT GCCTATGCTA ACAGCCAAGC CTTACTCCCA GCCCAAAAAC 
ACCCAGGAAG TCCTCAAGAC ATTTAAGGTA GATGGAAAGG TAAACCTCAA CGGGGTAGAA 
GAGCAGAAAG AAGAGGAACA TGGAAACCAG ACATTTGCCC CATCACTCAC CCGATCTCAG 
ATGTTTGAAG GTGTAGCCAG AGTTGATGTT CCTGTTGTTG AAATAAAGCA AGACGTAACT 
TCCATAGAGC TTAATCTGGC CAGACCAAAC ACCCTGAATT CCTTACAAGA ACCCAAAAGT 
CTCTGCGAAG ACAGAAATCA CATAAGCCAG GAGGATGAGT CAGATTCTCT GGTGCAATTA 
GAAAGAGCTC ACTCCGTGGA AATATTGTCA GAAGGTAACA CCCAATCAAG CAATGCAACC 
CAGGATGTAC CTGTTGTGAG CAGTATAGAG ATTCCACCAT TGACCATCAA TGGAAAGGAC 
ACTGTGGATG AGAGGAAAGT GGAAGCTGCC GAGTTGATCA TGCATTGGTC CCTGGAGCCG 
AATCATAAGG AAGCCAAAGA AAATTCTTTA TCGCCTTTAA AAACTTCTGA AAATTTAGAA 
GAATCCTTGC AGTTTAAAGA GAATGAATCA TCACACAAAG TGCAGCTAAA CATTACGCTT 
CAGAGCCGGA ACGCCTGGAG ACGCTCCCAG TTTTTTTTGC AGCCAGTGGA TACAGAATGT 
ACAGAAAATT CACCAGCACT TGTACCCAGT TTGAATTCTC CTAACCGATT CTGCACATGG 
GACCCTGAAG AAGAACGCAA ACGGCAAGAA AAATGGCAGC AGGAGCAGGA AAGGCTTCTC 
CAGGAAAGAA AGATAATTGA AGACACTGTG GTCCCACTGA CTTTGTCTCC AAATTCTAAT 
GCACAGTTTA ATATCTCTGA AAGAAATGGG AACTTCATTC CAGCACTTAC AGAACATGTC 
AAGAGAGATC AAGAAAATAA CAATGAAAAG CTCTTGACAG AGTCCAGCAG AACGATAAAG 
AATGAGACAC ATATGCACAG CAATGTAACA CAGCAACTGC TTTTGCATAC ACAGGAGGGG 
TCAATTCTTG GTGCTAAAGA GAAACAAATA AAGGGAAAAA CAGACAAGTG GGAAGCAGAA 
CAGTCCATCT GTATTATGGA CCGAGAGGAG CTGAGCAACC ATGTGCAGTC TAATGAATGT 
AATACACCCA CTAGGTTACC CTTGCAGGCA GGATGTAAAC AAGTGACGTC ATCAGAGTCA 
AGTCTGCCTG GGGATCTTGG ATCACCAAAA AATCCTATCA CTGAAAAAGA TGCTATTCAG 
AATATGTCGT CCATAATACC CACACGGGGA ACTGATTTCA AGGAAGATAC AATGAATTCT 
TCACAGTCCT CTACATCACA GTGCCAGTCA CCAAACAGGT CTGTTAGTGG GAAAAAACTG 
TGCTCCACAT GTGGACTTCC TCTAGGCAAG GGAGCTGCCA TGATAATTGA AACCCTCAGT 
CTTTATTTCC ACATCCAGTG CTTCAAGTGT GGACTGTGTA AGGGACAACT TGGGGATGCG 
ATCACCGGCA CAGACGTCAG AATTCGAAAT GGCCTACTGA ACTGCAATGA CTGTTACATG 
AAGTCTAGAA GTAAGGCGGC TGGCCACCCC ACCCCTTTGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

limch1 ( XM_012955699.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012811153.1 Xenopus tropicalis LIM and calponin homology domains 1 (limch1), transcript variant X8, mRNA.

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CloneID	OXa30870
Clone ID Related Accession (Same CDS sequence)	XM_012955699.2
Accession Version	XM_012955699.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5508bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1473177600000
Organism	Xenopus tropicalis(tropical clawed frog)
Product	LIM and calponin homology domains-containing protein 1 isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012955699.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCAGCT CCGCCACACT GCAAGGGGAA GAGATCCCCC CGCCCCAGGA GTCTCCCCCA 
GACACGGCTT GCAGGGAGGC ACAGAAGTGG ATTGAGCAAG TTACTGGCAA AAGCTTTGGC 
GACAAAGACT TCAGGACTGG ACTGGAGAAT GGAATCCTTC TGTGCGAGTT ATTAAATGCT 
ATTAAACCAG GACTCGTAAA AAAGATCAAC AGACTTCCTA CTCCCATTGC TGGATTGGAC 
AATATAACAT TATTCCTTAG AGGCTGCAAA GAGCTCGGGC TGAAAGAATC TCAGTTGTTT 
GACCCAGGTG ACCTACAGGA CACAGCAAAC AGGACAACAG CCAGGAATTC AGATTGCAAC 
AGAAAACTAA GAAATGTACT AATCACCGTG TACTGGCTGG GGAAAGCTGC AAGCAGCTGC 
TCGTCCTATA GTGGCAACAA CCTGGACTTA AAGGAGTTTG AGGGATTGCT GGCACAAATG 
AGAAAAGAAA ATGAGGAAAT AGAGAGTCCA AAACGCAGCA TACGGGACAG TGGCTACATA 
GATTGTTGGG ACTCTGAGAG AAGTGACTCC CTTTCTCCTC CCAGACATGG TAGAGATGAC 
TCCTTCGATA GTTTAGACTC ATTTGGATCT AGATCACAAC AGACACCATC TCCAGATGTA 
GTGCTGAGGG GAAGCAGCGA CGGCAGAGGA AGCGACTCTG AGTCTGACCT ACCTCACAGA 
AGGTTACCTG ATGTGCGAAA AGATGATATG TCTGCTAGAC GGGTATCCTA CGGTGAAAAC 
AAAGCATTTG TGCCTTTCAA CCAATACCTC CCCAACAAGA GCAACCAGAC CTCCTACATC 
CCGGCACCCC TGAGGAAGAA GAAGGCTGAG CGTGAGGACT ACCGGAAAAG CTGGAGCACA 
GCTACATCCC CCCTGGGGGG AGAGCGACCC TTCAGTCCCA AACAAAGTCC AGTCATTAAA 
GAAGAACAAA ACGAGTTGCA GTGTGAAGGA GAAGGGACCA AGCAGATGAT TGCCTTAAAA 
GAACCTTGCC TTGGAGCAGA AAATAGGAGT GAGAGAGTTG GTGAAAGTGA AGGGGGTGTC 
ATGGGACAAT CATCTGCTAA TGATGACATT CCAAAAGATG CAGAGGAAGT GAGAAAGCGC 
AAGCACCTTC AGAAAGCGGG TATCAAGGTC TTGCCCGCAA CAAAGCGCTA CAGCAGTCCA 
AGTCAATTTG CTGAAGACAT TGATGGCGCT CCAGACATTG TGCTGCGAAA AGAGAACACC 
TTCCTGAAGT CATATGAAGG GATGGGCTCT GATTCCGAAG AAGAAGAAGA GTGCAAAGTA 
CCTGATATAC AGCGAGATGA CTTGGCCACA AGGCGTGCAA GGATGAACCA ACCAAAAAGC 
CCAATGCAGT TTAATCAGTA TATACAGGGG TCATACTTAA ACAAAAACAG AGCATCAAGA 
AAGCTGAACA GTTCTAAGAC TTCCACTCCA GCTTCTCCAA AAATGTCTCA AGATATCAGG 
GAGTACCAAG GCCCCCAGTT ATTAAAGCCA TCACACGTCT GCAAAGAAAA GACATTTCCA 
ATACCCTCAG ACAATAAAGA GGATGCAGAA GCAGAGCAAG AAAAAGTTCC CGACATTCAG 
AAAGACGACC TGGCGAAACG CAAAGCTAAA GCTTCAGTTT CACTGCCAAA GGAACCATCG 
GCCTTTGCAG CGGTTTCAAT TACAAAGGCC GACATGGAAA CTTGGCAGAG GCTTCGAATG 
TCTTCTGAAG ATAGTGAGCA CTTGAATGAC AGGCAGGCTG ACCAGCAAGA GCAAACATCC 
AAGATTGATG TGACTAGAGC GACAAATGAT GACTTTGGGA GGAGGAAATT GTCTCTGCAA 
GAGAAAAGGG CTCGAAACAA ACAGAAGTTT GTACGCTTTG GCCCAGTGAC TGAAATTGAT 
CAGCAGAAGT GGGATAGGCT TTGTATAGCC AAGGCTGAAT CTGGCCAGGA AAGTGAAGAG 
ATGAAGGGCA GCATTACGTC TGATGAAACT GCCAGCCACA TATCTCCCTC AGCTTCTGCT 
CAGCGCTCCC AACCCCAGGA GGATGTTGGC ACAGGGCCAG TCTTGCCACA TGAGTCAAGA 
TCTTTTACAC CAGAGAGATT GGTTTCACCT GCTCCTCCTG AAAATGAAAG TGAAATTCAG 
CTCCTAGAAA CACAGCAAGA TGACATGATG GCAAGGAGAA CAGGTACATT CCAAAAATGC 
ACCAGAACCA GCAGAGACTC TCCTGTGTCC CACCAATATG CACTCCATAG CCAACAACAC 
GAAGAGAAGT CTGACTCGCT GGAACTCGAA GAGAAACAAG GACTCTTTCT GGATGTTAAC 
CCACAACTGA GAAAATTACT GCTGTCGGAC TTGGCAAATA CTAGTTCAAA AGCAACTAGC 
CCTACTTTGG GGAGCAAGTC TCTGAAAGTC ACCAAAGAAG CTGAAGATGT AAAAAATGAC 
ATGAAAGAAG GTGCCTTGAA CACAGGAAAG GATCCAAACT CTGTTATCCC AGAGGATAAC 
ACAATTGCAC CAGACCCAGA AAAAGATGAC ATGATGACAA GAAAAATCTC AGTGAGCCAG 
AAGCCATCTT GCGTCAGCCT TGGACACTTC CTTCCTGTTC CTTTTTCTTT GCAGCACAGA 
GAGGGGGAAA AGAAACCAAC AAGCTGCATC ATATCAAGCA CCAAAAAGTC CAAAAATGCA 
TCCAAAGCTG TTCCAGCCGA CATTTCTGAT GTTCCTTCTA AGCCCCTTGA CCAGAAAGAA 
ACAAAAGAAC ATGGTGAATT AGGAGATACG ACTTGCAAGC AAGCCTCCAA TAGTGTGGAG 
ACTCCCATGT GTACAGAGTT AGACAGCCAG CAACAGGATG GTCTTGAGAG CTCCACTCTA 
TCGATCAAAA TAAAGTCAGA TAATTGCACC CAAGCACTTG CAGTCGGCAG TTCTGACCTT 
CCTTGTAAGC TCCTCGGCCA ATGTGAAACA GAGGGACATG TTGAATTACG ATCCGATGTG 
ACTTGCGATC AAACCTCCAC TGAGATCATT AGTGATGGGA TTCCCACTGG TAAAGGGTTA 
GATACCCAGG CAGAAGACGC TCTTGAGACA TGTGACCATG AGTTATTCAG ATTCAGCCCA 
AGATCTGTTC TGTCTGATGA TGGAGAGAGC ATGAGCATGT GCGACATGAG GTACGAAGAA 
GAGGGGAGTA TGCAGCCTCA CAGCAGGGCA CACTATGAGC ATCTACAGCA TGTCAACAGC 
TTGCTCAAGG AGGAGGATGA CACATGGCAA GATGACCTAG CTAAGTGGAA GACACGGAGA 
AGAAGTGCAT CACAGGATCT GATCAAAAAA GAGGAACAGA GGAAGAAGAT GGAGAGAATG 
ATGAGCGGAG AGGATGAACT GTCGGAGCGT AGGAAAAGCA TAAAAACATA TAGAGAGATT 
GTGGAAGAAA AAGAACGTCG AGAGAAAGAA CTGCATGAAG CCTACAAAAA TGCCAAGTCA 
AGAGATGAGG CTGAACACAT ATTACAGAGC TACATTGAAA AGTTTACAAT TAGTGAAGCT 
GTTCTTGAGG GATTAGTAAT GCCAAAAATC TTAGAAAGAA GCCAGTCGGT GGAGCCAAGT 
TTATTGTCTT CATCAGAGGA CCCAAACATC CTGAAATACC TGAGGCAACA GTCTTTACCA 
TCACCAAAAT ACACTTCCAC TGTTGAAGCA ACTGTCTCCC CCAGCAGCCC ATGTGAAGCA 
AAAAGTCCAA CAGGTCTGAT ATCACCAAGC AAAAACATTA TCTCAAAGAC TGTGCCTATG 
CTAACAGCCA AGCCTTACTC CCAGCCCAAA AACACCCAGG AAGTCCTCAA GACATTTAAG 
GTAGATGGAA AGGTAAACCT CAACGGGGTA GAAGAGCAGA AAGAAGAGGA ACATGGAAAC 
CAGACATTTG CCCCATCACT CACCCGATCT CAGATGTTTG AAGGTGTAGC CAGAGTTGAT 
GTTCCTGTTG TTGAAATAAA GCAAGACGTA ACTTCCATAG AGCTTAATCT GGCCAGACCA 
AACACCCTGA ATTCCTTACA AGAACCCAAA AGTCTCTGCG AAGACAGAAA TCACATAAGC 
CAGGAGGATG AGTCAGATTC TCTGGTGCAA TTAGAAAGAG CTCACTCCGT GGAAATATTG 
TCAGAAGGTA ACACCCAATC AAGCAATGCA ACCCAGGATG TACCTGTTGT GAGCAGTATA 
GAGATTCCAC CATTGACCAT CAATGGAAAG GACACTGTGG ATGAGAGGAA AGTGGAAGCT 
GCCGAGTTGA TCATGCATTG GTCCCTGGAG CCGAATCATA AGGAAGCCAA AGAAAATTCT 
TTATCGCCTT TAAAAACTTC TGAAAATTTA GAAGAATCCT TGCAGTTTAA AGAGAATGAA 
TCATCACACA AAGTGCAGCT AAACATTACG CTTCAGAGCC GGAACGCCTG GAGACGCTCC 
CAGTTTTTTT TGCAGCCAGT GGATACAGAA TGTACAGAAA ATTCACCAGC ACTTGTACCC 
AGTTTGAATT CTCCTAACCG ATTCTGCACA TGGGACCCTG AAGAAGAACG CAAACGGCAA 
GAAAAATGGC AGCAGGAGCA GGAAAGGCTT CTCCAGGAGA GGTACCAGAG AGAGCAGGAA 
AAACTTAAAG AAGAATGGGA AAAAGCTCAG AAAGAAGTAG AAGAGGAGGA AAGGAAATAT 
TATGAGGAGG AAAGAAAGAT AATTGAAGAC ACTGTGGTCC CACTGACTTT GTCTCCAAAT 
TCTAATGCAC AGTTTAATAT CTCTGAAAGA AATGGGAACT TCATTCCAGC ACTTACAGAA 
CATGTCAAGA GAGATCAAGA AAATAACAAT GAAAAGCTCT TGACAGAGTC CAGCAGAACG 
ATAAAGAATG AGACACATAT GCACAGCAAT GTAACACAGC AACTGCTTTT GCATACACAG 
GAGGGGTCAA TTCTTGGTGC TAAAGAGAAA CAAATAAAGG GAAAAACAGA CAAGTGGGAA 
GCAGAACAGT CCATCTGTAT TATGGACCGA GAGGAGCTGA GCAACCATGT GCAGTCTAAT 
GAATGTAATA CACCCACTAG GTTACCCTTG CAGGCAGGAT GTAAACAAGT GACGTCATCA 
GAGTCAAGTC TGCCTGGGGA TCTTGGATCA CCAAAAAATC CTATCACTGA AAAAGATGCT 
ATTCAGAATA TGTCGTCCAT AATACCCACA CGGGGAACTG ATTTCAAGGA AGATACAATG 
AATTCTTCAC AGTCCTCTAC ATCACAGTGC CAGTCACCAA ACAGGTCTGT TAGTGGGAAA 
AAACTGTGCT CCACATGTGG ACTTCCTCTA GGCAAGGGAG CTGCCATGAT AATTGAAACC 
CTCAGTCTTT ATTTCCACAT CCAGTGCTTC AAGTGTGGAC TGTGTAAGGG ACAACTTGGG 
GATGCGATCA CCGGCACAGA CGTCAGAATT CGAAATGGCC TACTGAACTG CAATGACTGT 
TACATGAAGT CTAGAAGTAA GGCGGCTGGC CACCCCACCC CTTTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012811153.1
CDS	492..5999
Translation	MASSATLQGEEIPPPQESPPDTACREAQKWIEQVTGKSFGDKDFRTGLENGILLCELLNAIKPGLVKKINRLPTPIAGLDNITLFLRGCKELGLKESQLFDPGDLQDTANRTTARNSDCNRKLRNVLITVYWLGKAASSCSSYSGNNLDLKEFEGLLAQMRKENEEIESPKRSIRDSGYIDCWDSERSDSLSPPRHGRDDSFDSLDSFGSRSQQTPSPDVVLRGSSDGRGSDSESDLPHRRLPDVRKDDMSARRVSYGENKAFVPFNQYLPNKSNQTSYIPAPLRKKKAEREDYRKSWSTATSPLGGERPFSPKQSPVIKEEQNELQCEGEGTKQMIALKEPCLGAENRSERVGESEGGVMGQSSANDDIPKDAEEVRKRKHLQKAGIKVLPATKRYSSPSQFAEDIDGAPDIVLRKENTFLKSYEGMGSDSEEEEECKVPDIQRDDLATRRARMNQPKSPMQFNQYIQGSYLNKNRASRKLNSSKTSTPASPKMSQDIREYQGPQLLKPSHVCKEKTFPIPSDNKEDAEAEQEKVPDIQKDDLAKRKAKASVSLPKEPSAFAAVSITKADMETWQRLRMSSEDSEHLNDRQADQQEQTSKIDVTRATNDDFGRRKLSLQEKRARNKQKFVRFGPVTEIDQQKWDRLCIAKAESGQESEEMKGSITSDETASHISPSASAQRSQPQEDVGTGPVLPHESRSFTPERLVSPAPPENESEIQLLETQQDDMMARRTGTFQKCTRTSRDSPVSHQYALHSQQHEEKSDSLELEEKQGLFLDVNPQLRKLLLSDLANTSSKATSPTLGSKSLKVTKEAEDVKNDMKEGALNTGKDPNSVIPEDNTIAPDPEKDDMMTRKISVSQKPSCVSLGHFLPVPFSLQHREGEKKPTSCIISSTKKSKNASKAVPADISDVPSKPLDQKETKEHGELGDTTCKQASNSVETPMCTELDSQQQDGLESSTLSIKIKSDNCTQALAVGSSDLPCKLLGQCETEGHVELRSDVTCDQTSTEIISDGIPTGKGLDTQAEDALETCDHELFRFSPRSVLSDDGESMSMCDMRYEEEGSMQPHSRAHYEHLQHVNSLLKEEDDTWQDDLAKWKTRRRSASQDLIKKEEQRKKMERMMSGEDELSERRKSIKTYREIVEEKERREKELHEAYKNAKSRDEAEHILQSYIEKFTISEAVLEGLVMPKILERSQSVEPSLLSSSEDPNILKYLRQQSLPSPKYTSTVEATVSPSSPCEAKSPTGLISPSKNIISKTVPMLTAKPYSQPKNTQEVLKTFKVDGKVNLNGVEEQKEEEHGNQTFAPSLTRSQMFEGVARVDVPVVEIKQDVTSIELNLARPNTLNSLQEPKSLCEDRNHISQEDESDSLVQLERAHSVEILSEGNTQSSNATQDVPVVSSIEIPPLTINGKDTVDERKVEAAELIMHWSLEPNHKEAKENSLSPLKTSENLEESLQFKENESSHKVQLNITLQSRNAWRRSQFFLQPVDTECTENSPALVPSLNSPNRFCTWDPEEERKRQEKWQQEQERLLQERYQREQEKLKEEWEKAQKEVEEEERKYYEEERKIIEDTVVPLTLSPNSNAQFNISERNGNFIPALTEHVKRDQENNNEKLLTESSRTIKNETHMHSNVTQQLLLHTQEGSILGAKEKQIKGKTDKWEAEQSICIMDREELSNHVQSNECNTPTRLPLQAGCKQVTSSESSLPGDLGSPKNPITEKDAIQNMSSIIPTRGTDFKEDTMNSSQSSTSQCQSPNRSVSGKKLCSTCGLPLGKGAAMIIETLSLYFHIQCFKCGLCKGQLGDAITGTDVRIRNGLLNCNDCYMKSRSKAAGHPTPL

Target ORF information:

RefSeq Version	XM_012955699.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis LIM and calponin homology domains 1 (limch1), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012955699.2

ORF Insert Sequence:

ATGGCCAGCT CCGCCACACT GCAAGGGGAA GAGATCCCCC CGCCCCAGGA GTCTCCCCCA 
GACACGGCTT GCAGGGAGGC ACAGAAGTGG ATTGAGCAAG TTACTGGCAA AAGCTTTGGC 
GACAAAGACT TCAGGACTGG ACTGGAGAAT GGAATCCTTC TGTGCGAGTT ATTAAATGCT 
ATTAAACCAG GACTCGTAAA AAAGATCAAC AGACTTCCTA CTCCCATTGC TGGATTGGAC 
AATATAACAT TATTCCTTAG AGGCTGCAAA GAGCTCGGGC TGAAAGAATC TCAGTTGTTT 
GACCCAGGTG ACCTACAGGA CACAGCAAAC AGGACAACAG CCAGGAATTC AGATTGCAAC 
AGAAAACTAA GAAATGTACT AATCACCGTG TACTGGCTGG GGAAAGCTGC AAGCAGCTGC 
TCGTCCTATA GTGGCAACAA CCTGGACTTA AAGGAGTTTG AGGGATTGCT GGCACAAATG 
AGAAAAGAAA ATGAGGAAAT AGAGAGTCCA AAACGCAGCA TACGGGACAG TGGCTACATA 
GATTGTTGGG ACTCTGAGAG AAGTGACTCC CTTTCTCCTC CCAGACATGG TAGAGATGAC 
TCCTTCGATA GTTTAGACTC ATTTGGATCT AGATCACAAC AGACACCATC TCCAGATGTA 
GTGCTGAGGG GAAGCAGCGA CGGCAGAGGA AGCGACTCTG AGTCTGACCT ACCTCACAGA 
AGGTTACCTG ATGTGCGAAA AGATGATATG TCTGCTAGAC GGGTATCCTA CGGTGAAAAC 
AAAGCATTTG TGCCTTTCAA CCAATACCTC CCCAACAAGA GCAACCAGAC CTCCTACATC 
CCGGCACCCC TGAGGAAGAA GAAGGCTGAG CGTGAGGACT ACCGGAAAAG CTGGAGCACA 
GCTACATCCC CCCTGGGGGG AGAGCGACCC TTCAGTCCCA AACAAAGTCC AGTCATTAAA 
GAAGAACAAA ACGAGTTGCA GTGTGAAGGA GAAGGGACCA AGCAGATGAT TGCCTTAAAA 
GAACCTTGCC TTGGAGCAGA AAATAGGAGT GAGAGAGTTG GTGAAAGTGA AGGGGGTGTC 
ATGGGACAAT CATCTGCTAA TGATGACATT CCAAAAGATG CAGAGGAAGT GAGAAAGCGC 
AAGCACCTTC AGAAAGCGGG TATCAAGGTC TTGCCCGCAA CAAAGCGCTA CAGCAGTCCA 
AGTCAATTTG CTGAAGACAT TGATGGCGCT CCAGACATTG TGCTGCGAAA AGAGAACACC 
TTCCTGAAGT CATATGAAGG GATGGGCTCT GATTCCGAAG AAGAAGAAGA GTGCAAAGTA 
CCTGATATAC AGCGAGATGA CTTGGCCACA AGGCGTGCAA GGATGAACCA ACCAAAAAGC 
CCAATGCAGT TTAATCAGTA TATACAGGGG TCATACTTAA ACAAAAACAG AGCATCAAGA 
AAGCTGAACA GTTCTAAGAC TTCCACTCCA GCTTCTCCAA AAATGTCTCA AGATATCAGG 
GAGTACCAAG GCCCCCAGTT ATTAAAGCCA TCACACGTCT GCAAAGAAAA GACATTTCCA 
ATACCCTCAG ACAATAAAGA GGATGCAGAA GCAGAGCAAG AAAAAGTTCC CGACATTCAG 
AAAGACGACC TGGCGAAACG CAAAGCTAAA GCTTCAGTTT CACTGCCAAA GGAACCATCG 
GCCTTTGCAG CGGTTTCAAT TACAAAGGCC GACATGGAAA CTTGGCAGAG GCTTCGAATG 
TCTTCTGAAG ATAGTGAGCA CTTGAATGAC AGGCAGGCTG ACCAGCAAGA GCAAACATCC 
AAGATTGATG TGACTAGAGC GACAAATGAT GACTTTGGGA GGAGGAAATT GTCTCTGCAA 
GAGAAAAGGG CTCGAAACAA ACAGAAGTTT GTACGCTTTG GCCCAGTGAC TGAAATTGAT 
CAGCAGAAGT GGGATAGGCT TTGTATAGCC AAGGCTGAAT CTGGCCAGGA AAGTGAAGAG 
ATGAAGGGCA GCATTACGTC TGATGAAACT GCCAGCCACA TATCTCCCTC AGCTTCTGCT 
CAGCGCTCCC AACCCCAGGA GGATGTTGGC ACAGGGCCAG TCTTGCCACA TGAGTCAAGA 
TCTTTTACAC CAGAGAGATT GGTTTCACCT GCTCCTCCTG AAAATGAAAG TGAAATTCAG 
CTCCTAGAAA CACAGCAAGA TGACATGATG GCAAGGAGAA CAGGTACATT CCAAAAATGC 
ACCAGAACCA GCAGAGACTC TCCTGTGTCC CACCAATATG CACTCCATAG CCAACAACAC 
GAAGAGAAGT CTGACTCGCT GGAACTCGAA GAGAAACAAG GACTCTTTCT GGATGTTAAC 
CCACAACTGA GAAAATTACT GCTGTCGGAC TTGGCAAATA CTAGTTCAAA AGCAACTAGC 
CCTACTTTGG GGAGCAAGTC TCTGAAAGTC ACCAAAGAAG CTGAAGATGT AAAAAATGAC 
ATGAAAGAAG GTGCCTTGAA CACAGGAAAG GATCCAAACT CTGTTATCCC AGAGGATAAC 
ACAATTGCAC CAGACCCAGA AAAAGATGAC ATGATGACAA GAAAAATCTC AGTGAGCCAG 
AAGCCATCTT GCGTCAGCCT TGGACACTTC CTTCCTGTTC CTTTTTCTTT GCAGCACAGA 
GAGGGGGAAA AGAAACCAAC AAGCTGCATC ATATCAAGCA CCAAAAAGTC CAAAAATGCA 
TCCAAAGCTG TTCCAGCCGA CATTTCTGAT GTTCCTTCTA AGCCCCTTGA CCAGAAAGAA 
ACAAAAGAAC ATGGTGAATT AGGAGATACG ACTTGCAAGC AAGCCTCCAA TAGTGTGGAG 
ACTCCCATGT GTACAGAGTT AGACAGCCAG CAACAGGATG GTCTTGAGAG CTCCACTCTA 
TCGATCAAAA TAAAGTCAGA TAATTGCACC CAAGCACTTG CAGTCGGCAG TTCTGACCTT 
CCTTGTAAGC TCCTCGGCCA ATGTGAAACA GAGGGACATG TTGAATTACG ATCCGATGTG 
ACTTGCGATC AAACCTCCAC TGAGATCATT AGTGATGGGA TTCCCACTGG TAAAGGGTTA 
GATACCCAGG CAGAAGACGC TCTTGAGACA TGTGACCATG AGTTATTCAG ATTCAGCCCA 
AGATCTGTTC TGTCTGATGA TGGAGAGAGC ATGAGCATGT GCGACATGAG GTACGAAGAA 
GAGGGGAGTA TGCAGCCTCA CAGCAGGGCA CACTATGAGC ATCTACAGCA TGTCAACAGC 
TTGCTCAAGG AGGAGGATGA CACATGGCAA GATGACCTAG CTAAGTGGAA GACACGGAGA 
AGAAGTGCAT CACAGGATCT GATCAAAAAA GAGGAACAGA GGAAGAAGAT GGAGAGAATG 
ATGAGCGGAG AGGATGAACT GTCGGAGCGT AGGAAAAGCA TAAAAACATA TAGAGAGATT 
GTGGAAGAAA AAGAACGTCG AGAGAAAGAA CTGCATGAAG CCTACAAAAA TGCCAAGTCA 
AGAGATGAGG CTGAACACAT ATTACAGAGC TACATTGAAA AGTTTACAAT TAGTGAAGCT 
GTTCTTGAGG GATTAGTAAT GCCAAAAATC TTAGAAAGAA GCCAGTCGGT GGAGCCAAGT 
TTATTGTCTT CATCAGAGGA CCCAAACATC CTGAAATACC TGAGGCAACA GTCTTTACCA 
TCACCAAAAT ACACTTCCAC TGTTGAAGCA ACTGTCTCCC CCAGCAGCCC ATGTGAAGCA 
AAAAGTCCAA CAGGTCTGAT ATCACCAAGC AAAAACATTA TCTCAAAGAC TGTGCCTATG 
CTAACAGCCA AGCCTTACTC CCAGCCCAAA AACACCCAGG AAGTCCTCAA GACATTTAAG 
GTAGATGGAA AGGTAAACCT CAACGGGGTA GAAGAGCAGA AAGAAGAGGA ACATGGAAAC 
CAGACATTTG CCCCATCACT CACCCGATCT CAGATGTTTG AAGGTGTAGC CAGAGTTGAT 
GTTCCTGTTG TTGAAATAAA GCAAGACGTA ACTTCCATAG AGCTTAATCT GGCCAGACCA 
AACACCCTGA ATTCCTTACA AGAACCCAAA AGTCTCTGCG AAGACAGAAA TCACATAAGC 
CAGGAGGATG AGTCAGATTC TCTGGTGCAA TTAGAAAGAG CTCACTCCGT GGAAATATTG 
TCAGAAGGTA ACACCCAATC AAGCAATGCA ACCCAGGATG TACCTGTTGT GAGCAGTATA 
GAGATTCCAC CATTGACCAT CAATGGAAAG GACACTGTGG ATGAGAGGAA AGTGGAAGCT 
GCCGAGTTGA TCATGCATTG GTCCCTGGAG CCGAATCATA AGGAAGCCAA AGAAAATTCT 
TTATCGCCTT TAAAAACTTC TGAAAATTTA GAAGAATCCT TGCAGTTTAA AGAGAATGAA 
TCATCACACA AAGTGCAGCT AAACATTACG CTTCAGAGCC GGAACGCCTG GAGACGCTCC 
CAGTTTTTTT TGCAGCCAGT GGATACAGAA TGTACAGAAA ATTCACCAGC ACTTGTACCC 
AGTTTGAATT CTCCTAACCG ATTCTGCACA TGGGACCCTG AAGAAGAACG CAAACGGCAA 
GAAAAATGGC AGCAGGAGCA GGAAAGGCTT CTCCAGGAGA GGTACCAGAG AGAGCAGGAA 
AAACTTAAAG AAGAATGGGA AAAAGCTCAG AAAGAAGTAG AAGAGGAGGA AAGGAAATAT 
TATGAGGAGG AAAGAAAGAT AATTGAAGAC ACTGTGGTCC CACTGACTTT GTCTCCAAAT 
TCTAATGCAC AGTTTAATAT CTCTGAAAGA AATGGGAACT TCATTCCAGC ACTTACAGAA 
CATGTCAAGA GAGATCAAGA AAATAACAAT GAAAAGCTCT TGACAGAGTC CAGCAGAACG 
ATAAAGAATG AGACACATAT GCACAGCAAT GTAACACAGC AACTGCTTTT GCATACACAG 
GAGGGGTCAA TTCTTGGTGC TAAAGAGAAA CAAATAAAGG GAAAAACAGA CAAGTGGGAA 
GCAGAACAGT CCATCTGTAT TATGGACCGA GAGGAGCTGA GCAACCATGT GCAGTCTAAT 
GAATGTAATA CACCCACTAG GTTACCCTTG CAGGCAGGAT GTAAACAAGT GACGTCATCA 
GAGTCAAGTC TGCCTGGGGA TCTTGGATCA CCAAAAAATC CTATCACTGA AAAAGATGCT 
ATTCAGAATA TGTCGTCCAT AATACCCACA CGGGGAACTG ATTTCAAGGA AGATACAATG 
AATTCTTCAC AGTCCTCTAC ATCACAGTGC CAGTCACCAA ACAGGTCTGT TAGTGGGAAA 
AAACTGTGCT CCACATGTGG ACTTCCTCTA GGCAAGGGAG CTGCCATGAT AATTGAAACC 
CTCAGTCTTT ATTTCCACAT CCAGTGCTTC AAGTGTGGAC TGTGTAAGGG ACAACTTGGG 
GATGCGATCA CCGGCACAGA CGTCAGAATT CGAAATGGCC TACTGAACTG CAATGACTGT 
TACATGAAGT CTAGAAGTAA GGCGGCTGGC CACCCCACCC CTTTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

limch1 ( XM_004911217.4 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_004911274.2 Xenopus tropicalis LIM and calponin homology domains 1 (limch1), transcript variant X1, mRNA.

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CloneID	OXa49695
Clone ID Related Accession (Same CDS sequence)	XM_004911217.4
Accession Version	XM_004911217.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5775bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1576512000000
Organism	Xenopus tropicalis(tropical clawed frog)
Product	LIM and calponin homology domains-containing protein 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_004911217.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCAGCT CCGCCACACT GCAAGGGGAA GAGATCCCCC CGCCCCAGGA GTCTCCCCCA 
GACACGGCTT GCAGGGAGGC ACAGAAGTGG ATTGAGCAAG TTACTGGCAA AAGCTTTGGC 
GACAAAGACT TCAGGACTGG ACTGGAGAAT GGAATCCTTC TGTGCGAGTT ATTAAATGCT 
ATTAAACCAG GACTCGTAAA AAAGATCAAC AGACTTCCTA CTCCCATTGC TGGATTGGAC 
AATATAACAT TATTCCTTAG AGGCTGCAAA GAGCTCGGGC TGAAAGAATC TCAGTTGTTT 
GACCCAGGTG ACCTACAGGA CACAGCAAAC AGGACAACAG CCAGGAATTC AGATTGCAAC 
AGAAAACTAA GAAATGTACT AATCACCGTG TACTGGCTGG GGAAAGCTGC AAGCAGCTGC 
TCGTCCTATA GTGGCAACAA CCTGGACTTA AAGGAGTTTG AGGGATTGCT GGCACAAATG 
AGAAAAGAAA ATGAGGAAAT AGAGAGTCCA AAACGCAGCA TACGGGACAG TGGCTACATA 
GATTGTTGGG ACTCTGAGAG AAGTGACTCC CTTTCTCCTC CCAGACATGG TAGAGATGAC 
TCCTTCGATA GTTTAGACTC ATTTGGATCT AGATCACAAC AGACACCATC TCCAGATGTA 
GTGCTGAGGG GAAGCAGCGA CGGCAGAGGA AGCGACTCTG AGTCTGACCT ACCTCACAGA 
AGGTTACCTG ATGTGCGAAA AGATGATATG TCTGCTAGAC GGGTATCCTA CGGTGAAAAC 
AAAGCATTTG TGCCTTTCAA CCAATACCTC CCCAACAAGA GCAACCAGAC CTCCTACATC 
CCGGCACCCC TGAGGAAGAA GAAGGCTGAG CGTGAGGACT ACCGGAAAAG CTGGAGCACA 
GCTACATCCC CCCTGGGGGG AGAGCGACCC TTCAGTCCCA AACAAAGTCC AGTCATTAAA 
GAAGAACAAA ACGAGTTGCA GTGTGAAGGA GAAGGGACCA AGCAGATGAT TGCCTTAAAA 
GAACCTTGCC TTGGAGCAGA AAATAGGAGT GAGAGAGTTG GTGAAAGTGA AGGGGGTGTC 
ATGGGACAAT CATCTGCTAA TGATGACATT CCAAAAGATG CAGAGGAAGT GAGAAAGCGC 
AAGCACCTTC AGAAAGCGGG TATCAAGGTC TTGCCCGCAA CAAAGCGCTA CAGCAGTCCA 
AGTCAATTTG CTGAAGACAT TGATGGCGCT CCAGACATTG TGCTGCGAAA AGAGAACACC 
TTCCTGAAGT CATATGAAGG GATGGGCTCT GATTCCGAAG AAGAAGAAGA GTGCAAAGTA 
CCTGATATAC AGCGAGATGA CTTGGCCACA AGGCGTGCAA GGATGAACCA ACCAAAAAGC 
CCAATGCAGT TTAATCAGTA TATACAGGGG TCATACTTAA ACAAAAACAG AGCATCAAGA 
AAGCTGAACA GTTCTAAGAC TTCCACTCCA GCTTCTCCAA AAATGTCTCA AGATATCAGG 
GAGTACCAAG GCCCCCAGTT ATTAAAGCCA TCACACGTCT GCAAAGAAAA GACATTTCCA 
ATACCCTCAG ACAATAAAGA GGATGCAGAA GCAGAGCAAG AAAAAGTTCC CGACATTCAG 
AAAGACGACC TGGCGAAACG CAAAGCTAAA GCTTCAGTTT CACTGCCAAA GGAACCATCG 
GCCTTTGCAG CGGTTTCAAT TACAAAGGCC GACATGGAAA CTTGGCAGAG GCTTCGAATG 
TCTTCTGAAG ATAGTGAGCA CTTGAATGAC AGGCAGGCTG ACCAGCAAGA GCAAACATCC 
AAGATTGATG TGACTAGAGC GACAAATGAT GACTTTGGGA GGAGGAAATT GTCTCTGCAA 
GAGAAAAGGG CTCGAAACAA ACAGAAGTTT GTACGCTTTG GCCCAGTGAC TGAAATTGAT 
CAGCAGAAGT GGGATAGGCT TTGTATAGCC AAGGCTGAAT CTGGCCAGGA AAGTGAAGAG 
ATGAAGGGCA GCATTACGTC TGATGAAACT GCCAGCCACA TATCTCCCTC AGCTTCTGCT 
CAGCGCTCCC AACCCCAGGA GGATGTTGGC ACAGGGCCAG TCTTGCCACA TGAGTCAAGA 
TCTTTTACAC CAGAGAGATT GGTTTCACCT GCTCCTCCTG AAAATGAAAG TGAAATTCAG 
CTCCTAGAAA CACAGCAAGA TGACATGATG GCAAGGAGAA CAGGTACATT CCAAAAATGC 
GCCAGAACCA GCAGAGACTC TCCTGTGTCC CACCAATATG CACTCCATAG CCAACAACAC 
GAAGAGAAGT CTGACTCGCT GGAACTCGAA GAGAAACAAG GACTCTTTCT GGATGTTAAC 
CCACAACTGA GAAAATTACT GCTGTCGGAC TTGGCAAATA CTAGTTCAAA AGCAACTAGC 
CCTACTTTGG GGAGCAAGTC TCTGAAAGTC ACCAAAGAAG CTGAAGATGT AAAAAATGAC 
ATGAAAGAAG GTGCCTTGAA CACAGGAAAG GATCCAAACT CTGTTATCCC AGAGGATAAC 
ACAATTGCAC CAGACCCAGA AAAAGATGAC ATGATGACAA GAAAAATCTC AGTGAGCCAG 
AAGCCATCTT GCGTCAGCCT TGGACACTTC CTTCCTGTTC CTTTTTCTTT GCAGCGCAGA 
GAGGGGGAAA AGAAACCAAC AAGCTGCATC ATATCAAGCA CCAAAAAGTC CAAAAATGCA 
TCCAAAGCTG TTCCAGCCGA CATTTCTGAT GTTCCTTCTA AGCCCCTTGA CCAGAAAGAA 
ACAAAAGAAC ATGGTGAATT AGGAGATACG ACTTGCAAGC AAGCCTCCAA TAGTGTGGAG 
ACTCCCATGT GTACAGAGTT AGACAGCCAG CAACAGGATG GTCTTGAGAG GAGAATGATA 
CTGTCTACTG ACTTGGCCAA TACTGAGTTA AAAATAACTC CTCCTACTTT GGCCAACAAC 
TCTCCAACCA TAACAAAAGT ACAAGAGCCA GATGCAACCA TCTCAGAGGA CAATACAACT 
GCACCAAACC CTGAAAAAGA TGATATGATG ACCAGAAGAA TCTCTGTAAC CCAGAAACCA 
TCGTGTGTAG GTCTTGGCCA CTTCCTTCCA GTTCCATTTT CTTTGCAGCA AAAAGTTGAG 
GAAAAGAAAT CAACGAGCTC CACTCTATCG ATCAAAATAA AGTCAGATAA TTGCACCCAA 
GCACTTGCAG TCGGCAGTTC TGACCTTCCT TGTAAGCTCC TCGGCCAATG TGAAACAGAG 
GGACATGTTG AATTACGATC CGATGTGACT TGCGATCAAA CCTCCACTGA GATCATTAGT 
GATGGGATTC CCACTGGTAA AGGGTTAGAT ACCCAGGCAG AAGACGCTCT TGAGACATGT 
GACCATGAGT TATTCAGATT CAGCCCAAGA TCTGTTCTGT CTGATGATGG AGAGAGCATG 
AGCATGTGCG ACATGAGGTA CGAAGAAGAG GGAAGTATGC AGCCTCACAG CAGGGCACAC 
TATGAGCATC TACAGCATGT CAACAGCTTG CTCAAGGAGG AGGATGACAC ATGGCAAGAT 
GACCTAGCTA AGTGGAAGAC ACGGAGAAGA AGTGCATCAC AGGATCTGAT CAAAAAAGAG 
GAACAGAGGA AGAAGATGGA GAGAATGATG AGCGGAGAGG ATGAACTGTC GGAGCGTAGG 
AAAAGCATAA AAACATATAG AGAGATTGTG GAAGAAAAAG AACGTCGAGA GAAAGAACTG 
CATGAAGCCT ACAAAAATGC CAAGTCAAGA GATGAGGCTG AACACATATT ACAGAGCTAC 
ATTGAAAAGT TTACAATTAG TGAAGCTGTT CTTGAGGGAT TAGTAATGCC AAAAATCTTA 
GAAAGAAGCC AGTCGGTGGA GCCAAGTTTA TTGTCTTCAT CAGAGGACCC AAACATCCTG 
AAATACCTGA GGCAACAGTC TTTACCATCA CCAAAATACA CTTCCACTGT TGAAGCAACT 
GTCTCCCCCA GCAGCCCATG TGAAGCAAAA AGTCCAACAG GTCTGATATC ACCAAGCAAA 
AACATTATCT CAAAGACTGT GCCTATGCTA ACAGCCAAGC CTTACTCCCA GCCCAAAAAC 
ACCCAGGAAG TCCTCAAGAC ATTTAAGGTA GATGGAAAGG TAAACCTCAA CGGGGTAGAA 
GAGCAGAAAG AAGAGGAACA TGGAAACCAG ACATTTGCCC CATCACTCAC CCGATCTCAG 
ATGTTTGAAG GTGTAGCCAG AGTTGATGTT CCTGTTGTTG AAATAAAGCA AGACGTAACT 
TCCATAGAGC TTAATCTGGC CAGACCAAAC ACCCTGAATT CCTTACAAGA ACCCAAAAGT 
CTCTGCGAAG ACAGAAATCA CATAAGCCAG GAGGATGAGT CAGATTCTCT GGTGCAATTA 
GAAAGAGCTC ACTCCGTGGA AATATTGTCA GAAGGTAACA CCCAATCAAG CAATGCAACC 
CAGGATGTAC CTGTTGTGAG CAGTATAGAG ATTCCACCAT TGACCATCAA TGGAAAGGAC 
ACTGTGGATG AGAGGAAAGT GGAAGCTGCC GAGTTGATCA TGCATTGGTC CCTGGAGCCG 
AATCATAAGG AAGCCAAAGA AAATTCTTTA TCGCCTTTAA AAACTTCTGA AAATTTAGAA 
GAATCCTTGC AGTTTAAAGA GAATGAATCA TCACACAAAG TGCAGCTAAA CATTACGCTT 
CAGAGCCGGA ACGCCTGGAG ACGCTCCCAG TTTTTTTTGC AGCCAGTGGA TACAGAATGT 
ACAGAAAATT CACCAGCACT TGTACCCAGT TTGAATTCTC CTAACCGATT CTGCACATGG 
GACCCTGAAG AAGAACGCAA ACGGCAAGAA AAATGGCAGC AGGAGCAGGA AAGGCTTCTC 
CAGGAGAGGT ACCAGAGAGA GCAGGAAAAA CTTAAAGAAG AATGGGAAAA AGCTCAGAAA 
GAAGTAGAAG AGGAGGAAAG GAAATATTAT GAGGAGGAAA GAAAGATAAT TGAAGACACT 
GTGGTCCCAC TGACTTTGTC TCCAAATTCT AATGCACAGT TTAATATCTC TGAAAGAAAT 
GGGAACTTCA TTCCAGCACT TACAGAACAT GTCAAGAGAG ATCAAGAAAA TAACAATGAA 
AAGCTCTTGA CAGAGTCCAG CAGAACGATA AAGAATGAGA CACATATGCA CAGCAATGTA 
ACACAGCAAC TGCTTTTGCA TACACAGGAG GGGTCAATTC TTGGTGCTAA AGAGAAACAA 
ATAAAGGGAA AAACAGACAA GTGGGAAGCA GAACAGTCCA TCTGTATTAT GGACCGAGAG 
GAGCTGAGCA ACCATGTGCA GTCTAATGAA TGTAATACAC CCACTAGGTT ACCCTTGCAG 
GCAGGATGTA AACAAGTGAC GTCATCAGAG TCAAGTCTGC CTGGGGATCT TGGATCACCA 
AAAAATCCTA TCACTGAAAA AGATGCTATT CAGAATATGT CGTCCATAAT ACCCACACGG 
GGAACTGATT TCAAGGAAGA TACAATGAAT TCTTCACAGT CCTCTACATC ACAGTGCCAG 
TCACCAAACA GGTCTGTTAG TGGGAAAAAA CTGTGCTCCA CATGTGGACT TCCTCTAGGC 
AAGGGAGCTG CCATGATAAT TGAAACCCTC AGTCTTTATT TCCACATCCA GTGCTTCAAG 
TGTGGACTGT GTAAGGGACA ACTTGGGGAT GCGATCACCG GCACAGACGT CAGAATTCGA 
AATGGCCTAC TGAACTGCAA TGACTGTTAC ATGAAGTCTA GAAGTAAGGC GGCTGGCCAC 
CCCACCCCTT TGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004911274.2
CDS	491..6265
Translation	MASSATLQGEEIPPPQESPPDTACREAQKWIEQVTGKSFGDKDFRTGLENGILLCELLNAIKPGLVKKINRLPTPIAGLDNITLFLRGCKELGLKESQLFDPGDLQDTANRTTARNSDCNRKLRNVLITVYWLGKAASSCSSYSGNNLDLKEFEGLLAQMRKENEEIESPKRSIRDSGYIDCWDSERSDSLSPPRHGRDDSFDSLDSFGSRSQQTPSPDVVLRGSSDGRGSDSESDLPHRRLPDVRKDDMSARRVSYGENKAFVPFNQYLPNKSNQTSYIPAPLRKKKAEREDYRKSWSTATSPLGGERPFSPKQSPVIKEEQNELQCEGEGTKQMIALKEPCLGAENRSERVGESEGGVMGQSSANDDIPKDAEEVRKRKHLQKAGIKVLPATKRYSSPSQFAEDIDGAPDIVLRKENTFLKSYEGMGSDSEEEEECKVPDIQRDDLATRRARMNQPKSPMQFNQYIQGSYLNKNRASRKLNSSKTSTPASPKMSQDIREYQGPQLLKPSHVCKEKTFPIPSDNKEDAEAEQEKVPDIQKDDLAKRKAKASVSLPKEPSAFAAVSITKADMETWQRLRMSSEDSEHLNDRQADQQEQTSKIDVTRATNDDFGRRKLSLQEKRARNKQKFVRFGPVTEIDQQKWDRLCIAKAESGQESEEMKGSITSDETASHISPSASAQRSQPQEDVGTGPVLPHESRSFTPERLVSPAPPENESEIQLLETQQDDMMARRTGTFQKCARTSRDSPVSHQYALHSQQHEEKSDSLELEEKQGLFLDVNPQLRKLLLSDLANTSSKATSPTLGSKSLKVTKEAEDVKNDMKEGALNTGKDPNSVIPEDNTIAPDPEKDDMMTRKISVSQKPSCVSLGHFLPVPFSLQRREGEKKPTSCIISSTKKSKNASKAVPADISDVPSKPLDQKETKEHGELGDTTCKQASNSVETPMCTELDSQQQDGLERRMILSTDLANTELKITPPTLANNSPTITKVQEPDATISEDNTTAPNPEKDDMMTRRISVTQKPSCVGLGHFLPVPFSLQQKVEEKKSTSSTLSIKIKSDNCTQALAVGSSDLPCKLLGQCETEGHVELRSDVTCDQTSTEIISDGIPTGKGLDTQAEDALETCDHELFRFSPRSVLSDDGESMSMCDMRYEEEGSMQPHSRAHYEHLQHVNSLLKEEDDTWQDDLAKWKTRRRSASQDLIKKEEQRKKMERMMSGEDELSERRKSIKTYREIVEEKERREKELHEAYKNAKSRDEAEHILQSYIEKFTISEAVLEGLVMPKILERSQSVEPSLLSSSEDPNILKYLRQQSLPSPKYTSTVEATVSPSSPCEAKSPTGLISPSKNIISKTVPMLTAKPYSQPKNTQEVLKTFKVDGKVNLNGVEEQKEEEHGNQTFAPSLTRSQMFEGVARVDVPVVEIKQDVTSIELNLARPNTLNSLQEPKSLCEDRNHISQEDESDSLVQLERAHSVEILSEGNTQSSNATQDVPVVSSIEIPPLTINGKDTVDERKVEAAELIMHWSLEPNHKEAKENSLSPLKTSENLEESLQFKENESSHKVQLNITLQSRNAWRRSQFFLQPVDTECTENSPALVPSLNSPNRFCTWDPEEERKRQEKWQQEQERLLQERYQREQEKLKEEWEKAQKEVEEEERKYYEEERKIIEDTVVPLTLSPNSNAQFNISERNGNFIPALTEHVKRDQENNNEKLLTESSRTIKNETHMHSNVTQQLLLHTQEGSILGAKEKQIKGKTDKWEAEQSICIMDREELSNHVQSNECNTPTRLPLQAGCKQVTSSESSLPGDLGSPKNPITEKDAIQNMSSIIPTRGTDFKEDTMNSSQSSTSQCQSPNRSVSGKKLCSTCGLPLGKGAAMIIETLSLYFHIQCFKCGLCKGQLGDAITGTDVRIRNGLLNCNDCYMKSRSKAAGHPTPL

Target ORF information:

RefSeq Version	XM_004911217.4
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis LIM and calponin homology domains 1 (limch1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004911217.4

ORF Insert Sequence:

ATGGCCAGCT CCGCCACACT GCAAGGGGAA GAGATCCCCC CGCCCCAGGA GTCTCCCCCA 
GACACGGCTT GCAGGGAGGC ACAGAAGTGG ATTGAGCAAG TTACTGGCAA AAGCTTTGGC 
GACAAAGACT TCAGGACTGG ACTGGAGAAT GGAATCCTTC TGTGCGAGTT ATTAAATGCT 
ATTAAACCAG GACTCGTAAA AAAGATCAAC AGACTTCCTA CTCCCATTGC TGGATTGGAC 
AATATAACAT TATTCCTTAG AGGCTGCAAA GAGCTCGGGC TGAAAGAATC TCAGTTGTTT 
GACCCAGGTG ACCTACAGGA CACAGCAAAC AGGACAACAG CCAGGAATTC AGATTGCAAC 
AGAAAACTAA GAAATGTACT AATCACCGTG TACTGGCTGG GGAAAGCTGC AAGCAGCTGC 
TCGTCCTATA GTGGCAACAA CCTGGACTTA AAGGAGTTTG AGGGATTGCT GGCACAAATG 
AGAAAAGAAA ATGAGGAAAT AGAGAGTCCA AAACGCAGCA TACGGGACAG TGGCTACATA 
GATTGTTGGG ACTCTGAGAG AAGTGACTCC CTTTCTCCTC CCAGACATGG TAGAGATGAC 
TCCTTCGATA GTTTAGACTC ATTTGGATCT AGATCACAAC AGACACCATC TCCAGATGTA 
GTGCTGAGGG GAAGCAGCGA CGGCAGAGGA AGCGACTCTG AGTCTGACCT ACCTCACAGA 
AGGTTACCTG ATGTGCGAAA AGATGATATG TCTGCTAGAC GGGTATCCTA CGGTGAAAAC 
AAAGCATTTG TGCCTTTCAA CCAATACCTC CCCAACAAGA GCAACCAGAC CTCCTACATC 
CCGGCACCCC TGAGGAAGAA GAAGGCTGAG CGTGAGGACT ACCGGAAAAG CTGGAGCACA 
GCTACATCCC CCCTGGGGGG AGAGCGACCC TTCAGTCCCA AACAAAGTCC AGTCATTAAA 
GAAGAACAAA ACGAGTTGCA GTGTGAAGGA GAAGGGACCA AGCAGATGAT TGCCTTAAAA 
GAACCTTGCC TTGGAGCAGA AAATAGGAGT GAGAGAGTTG GTGAAAGTGA AGGGGGTGTC 
ATGGGACAAT CATCTGCTAA TGATGACATT CCAAAAGATG CAGAGGAAGT GAGAAAGCGC 
AAGCACCTTC AGAAAGCGGG TATCAAGGTC TTGCCCGCAA CAAAGCGCTA CAGCAGTCCA 
AGTCAATTTG CTGAAGACAT TGATGGCGCT CCAGACATTG TGCTGCGAAA AGAGAACACC 
TTCCTGAAGT CATATGAAGG GATGGGCTCT GATTCCGAAG AAGAAGAAGA GTGCAAAGTA 
CCTGATATAC AGCGAGATGA CTTGGCCACA AGGCGTGCAA GGATGAACCA ACCAAAAAGC 
CCAATGCAGT TTAATCAGTA TATACAGGGG TCATACTTAA ACAAAAACAG AGCATCAAGA 
AAGCTGAACA GTTCTAAGAC TTCCACTCCA GCTTCTCCAA AAATGTCTCA AGATATCAGG 
GAGTACCAAG GCCCCCAGTT ATTAAAGCCA TCACACGTCT GCAAAGAAAA GACATTTCCA 
ATACCCTCAG ACAATAAAGA GGATGCAGAA GCAGAGCAAG AAAAAGTTCC CGACATTCAG 
AAAGACGACC TGGCGAAACG CAAAGCTAAA GCTTCAGTTT CACTGCCAAA GGAACCATCG 
GCCTTTGCAG CGGTTTCAAT TACAAAGGCC GACATGGAAA CTTGGCAGAG GCTTCGAATG 
TCTTCTGAAG ATAGTGAGCA CTTGAATGAC AGGCAGGCTG ACCAGCAAGA GCAAACATCC 
AAGATTGATG TGACTAGAGC GACAAATGAT GACTTTGGGA GGAGGAAATT GTCTCTGCAA 
GAGAAAAGGG CTCGAAACAA ACAGAAGTTT GTACGCTTTG GCCCAGTGAC TGAAATTGAT 
CAGCAGAAGT GGGATAGGCT TTGTATAGCC AAGGCTGAAT CTGGCCAGGA AAGTGAAGAG 
ATGAAGGGCA GCATTACGTC TGATGAAACT GCCAGCCACA TATCTCCCTC AGCTTCTGCT 
CAGCGCTCCC AACCCCAGGA GGATGTTGGC ACAGGGCCAG TCTTGCCACA TGAGTCAAGA 
TCTTTTACAC CAGAGAGATT GGTTTCACCT GCTCCTCCTG AAAATGAAAG TGAAATTCAG 
CTCCTAGAAA CACAGCAAGA TGACATGATG GCAAGGAGAA CAGGTACATT CCAAAAATGC 
GCCAGAACCA GCAGAGACTC TCCTGTGTCC CACCAATATG CACTCCATAG CCAACAACAC 
GAAGAGAAGT CTGACTCGCT GGAACTCGAA GAGAAACAAG GACTCTTTCT GGATGTTAAC 
CCACAACTGA GAAAATTACT GCTGTCGGAC TTGGCAAATA CTAGTTCAAA AGCAACTAGC 
CCTACTTTGG GGAGCAAGTC TCTGAAAGTC ACCAAAGAAG CTGAAGATGT AAAAAATGAC 
ATGAAAGAAG GTGCCTTGAA CACAGGAAAG GATCCAAACT CTGTTATCCC AGAGGATAAC 
ACAATTGCAC CAGACCCAGA AAAAGATGAC ATGATGACAA GAAAAATCTC AGTGAGCCAG 
AAGCCATCTT GCGTCAGCCT TGGACACTTC CTTCCTGTTC CTTTTTCTTT GCAGCGCAGA 
GAGGGGGAAA AGAAACCAAC AAGCTGCATC ATATCAAGCA CCAAAAAGTC CAAAAATGCA 
TCCAAAGCTG TTCCAGCCGA CATTTCTGAT GTTCCTTCTA AGCCCCTTGA CCAGAAAGAA 
ACAAAAGAAC ATGGTGAATT AGGAGATACG ACTTGCAAGC AAGCCTCCAA TAGTGTGGAG 
ACTCCCATGT GTACAGAGTT AGACAGCCAG CAACAGGATG GTCTTGAGAG GAGAATGATA 
CTGTCTACTG ACTTGGCCAA TACTGAGTTA AAAATAACTC CTCCTACTTT GGCCAACAAC 
TCTCCAACCA TAACAAAAGT ACAAGAGCCA GATGCAACCA TCTCAGAGGA CAATACAACT 
GCACCAAACC CTGAAAAAGA TGATATGATG ACCAGAAGAA TCTCTGTAAC CCAGAAACCA 
TCGTGTGTAG GTCTTGGCCA CTTCCTTCCA GTTCCATTTT CTTTGCAGCA AAAAGTTGAG 
GAAAAGAAAT CAACGAGCTC CACTCTATCG ATCAAAATAA AGTCAGATAA TTGCACCCAA 
GCACTTGCAG TCGGCAGTTC TGACCTTCCT TGTAAGCTCC TCGGCCAATG TGAAACAGAG 
GGACATGTTG AATTACGATC CGATGTGACT TGCGATCAAA CCTCCACTGA GATCATTAGT 
GATGGGATTC CCACTGGTAA AGGGTTAGAT ACCCAGGCAG AAGACGCTCT TGAGACATGT 
GACCATGAGT TATTCAGATT CAGCCCAAGA TCTGTTCTGT CTGATGATGG AGAGAGCATG 
AGCATGTGCG ACATGAGGTA CGAAGAAGAG GGAAGTATGC AGCCTCACAG CAGGGCACAC 
TATGAGCATC TACAGCATGT CAACAGCTTG CTCAAGGAGG AGGATGACAC ATGGCAAGAT 
GACCTAGCTA AGTGGAAGAC ACGGAGAAGA AGTGCATCAC AGGATCTGAT CAAAAAAGAG 
GAACAGAGGA AGAAGATGGA GAGAATGATG AGCGGAGAGG ATGAACTGTC GGAGCGTAGG 
AAAAGCATAA AAACATATAG AGAGATTGTG GAAGAAAAAG AACGTCGAGA GAAAGAACTG 
CATGAAGCCT ACAAAAATGC CAAGTCAAGA GATGAGGCTG AACACATATT ACAGAGCTAC 
ATTGAAAAGT TTACAATTAG TGAAGCTGTT CTTGAGGGAT TAGTAATGCC AAAAATCTTA 
GAAAGAAGCC AGTCGGTGGA GCCAAGTTTA TTGTCTTCAT CAGAGGACCC AAACATCCTG 
AAATACCTGA GGCAACAGTC TTTACCATCA CCAAAATACA CTTCCACTGT TGAAGCAACT 
GTCTCCCCCA GCAGCCCATG TGAAGCAAAA AGTCCAACAG GTCTGATATC ACCAAGCAAA 
AACATTATCT CAAAGACTGT GCCTATGCTA ACAGCCAAGC CTTACTCCCA GCCCAAAAAC 
ACCCAGGAAG TCCTCAAGAC ATTTAAGGTA GATGGAAAGG TAAACCTCAA CGGGGTAGAA 
GAGCAGAAAG AAGAGGAACA TGGAAACCAG ACATTTGCCC CATCACTCAC CCGATCTCAG 
ATGTTTGAAG GTGTAGCCAG AGTTGATGTT CCTGTTGTTG AAATAAAGCA AGACGTAACT 
TCCATAGAGC TTAATCTGGC CAGACCAAAC ACCCTGAATT CCTTACAAGA ACCCAAAAGT 
CTCTGCGAAG ACAGAAATCA CATAAGCCAG GAGGATGAGT CAGATTCTCT GGTGCAATTA 
GAAAGAGCTC ACTCCGTGGA AATATTGTCA GAAGGTAACA CCCAATCAAG CAATGCAACC 
CAGGATGTAC CTGTTGTGAG CAGTATAGAG ATTCCACCAT TGACCATCAA TGGAAAGGAC 
ACTGTGGATG AGAGGAAAGT GGAAGCTGCC GAGTTGATCA TGCATTGGTC CCTGGAGCCG 
AATCATAAGG AAGCCAAAGA AAATTCTTTA TCGCCTTTAA AAACTTCTGA AAATTTAGAA 
GAATCCTTGC AGTTTAAAGA GAATGAATCA TCACACAAAG TGCAGCTAAA CATTACGCTT 
CAGAGCCGGA ACGCCTGGAG ACGCTCCCAG TTTTTTTTGC AGCCAGTGGA TACAGAATGT 
ACAGAAAATT CACCAGCACT TGTACCCAGT TTGAATTCTC CTAACCGATT CTGCACATGG 
GACCCTGAAG AAGAACGCAA ACGGCAAGAA AAATGGCAGC AGGAGCAGGA AAGGCTTCTC 
CAGGAGAGGT ACCAGAGAGA GCAGGAAAAA CTTAAAGAAG AATGGGAAAA AGCTCAGAAA 
GAAGTAGAAG AGGAGGAAAG GAAATATTAT GAGGAGGAAA GAAAGATAAT TGAAGACACT 
GTGGTCCCAC TGACTTTGTC TCCAAATTCT AATGCACAGT TTAATATCTC TGAAAGAAAT 
GGGAACTTCA TTCCAGCACT TACAGAACAT GTCAAGAGAG ATCAAGAAAA TAACAATGAA 
AAGCTCTTGA CAGAGTCCAG CAGAACGATA AAGAATGAGA CACATATGCA CAGCAATGTA 
ACACAGCAAC TGCTTTTGCA TACACAGGAG GGGTCAATTC TTGGTGCTAA AGAGAAACAA 
ATAAAGGGAA AAACAGACAA GTGGGAAGCA GAACAGTCCA TCTGTATTAT GGACCGAGAG 
GAGCTGAGCA ACCATGTGCA GTCTAATGAA TGTAATACAC CCACTAGGTT ACCCTTGCAG 
GCAGGATGTA AACAAGTGAC GTCATCAGAG TCAAGTCTGC CTGGGGATCT TGGATCACCA 
AAAAATCCTA TCACTGAAAA AGATGCTATT CAGAATATGT CGTCCATAAT ACCCACACGG 
GGAACTGATT TCAAGGAAGA TACAATGAAT TCTTCACAGT CCTCTACATC ACAGTGCCAG 
TCACCAAACA GGTCTGTTAG TGGGAAAAAA CTGTGCTCCA CATGTGGACT TCCTCTAGGC 
AAGGGAGCTG CCATGATAAT TGAAACCCTC AGTCTTTATT TCCACATCCA GTGCTTCAAG 
TGTGGACTGT GTAAGGGACA ACTTGGGGAT GCGATCACCG GCACAGACGT CAGAATTCGA 
AATGGCCTAC TGAACTGCAA TGACTGTTAC ATGAAGTCTA GAAGTAAGGC GGCTGGCCAC 
CCCACCCCTT TGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

limch1 ( XM_004911218.4 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_004911275.2 Xenopus tropicalis LIM and calponin homology domains 1 (limch1), transcript variant X2, mRNA.

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CloneID	OXa49696
Clone ID Related Accession (Same CDS sequence)	XM_004911218.4
Accession Version	XM_004911218.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5769bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1576512000000
Organism	Xenopus tropicalis(tropical clawed frog)
Product	LIM and calponin homology domains-containing protein 1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_004911218.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCAGCT CCGCCACACT GCAAGGGGAA GAGATCCCCC CGCCCCAGGA GTCTCCCCCA 
GACACGGCTT GCAGGGAGGC ACAGAAGTGG ATTGAGCAAG TTACTGGCAA AAGCTTTGGC 
GACAAAGACT TCAGGACTGG ACTGGAGAAT GGAATCCTTC TGTGCGAGTT ATTAAATGCT 
ATTAAACCAG GACTCGTAAA AAAGATCAAC AGACTTCCTA CTCCCATTGC TGGATTGGAC 
AATATAACAT TATTCCTTAG AGGCTGCAAA GAGCTCGGGC TGAAAGAATC TCAGTTGTTT 
GACCCAGGTG ACCTACAGGA CACAGCAAAC AGGACAACAG CCAGGAATTC AGATTGCAAC 
AGAAAACTAA GAAATGTACT AATCACCGTG TACTGGCTGG GGAAAGCTGC AAGCAGCTGC 
TCGTCCTATA GTGGCAACAA CCTGGACTTA AAGGAGTTTG AGGGATTGCT GGCACAAATG 
AGAAAAGAAA ATGAGGAAAT AGAGAGTCCA AAACGCAGCA TACGGGACAG TGGCTACATA 
GATTGTTGGG ACTCTGAGAG AAGTGACTCC CTTTCTCCTC CCAGACATGG TAGAGATGAC 
TCCTTCGATA GTTTAGACTC ATTTGGATCT AGATCACAAC AGACACCATC TCCAGATGTA 
GTGCTGAGGG GAAGCAGCGA CGGCAGAGGA AGCGACTCTG AGTCTGACCT ACCTCACAGA 
AGGTTACCTG ATGTGCGAAA AGATGATATG TCTGCTAGAC GGGTATCCTA CGGTGAAAAC 
AAAGCATTTG TGCCTTTCAA CCAATACCTC CCCAACAAGA GCAACCAGAC CTCCTACATC 
CCGGCACCCC TGAGGAAGAA GAAGGCTGAG CGTGAGGACT ACCGGAAAAG CTGGAGCACA 
GCTACATCCC CCCTGGGGGG AGAGCGACCC TTCAGTCCCA AACAAAGTCC AGTCATTAAA 
GAAGAACAAA ACGAGTTGCA GTGTGAAGGA GAAGGGACCA AGCAGATGAT TGCCTTAAAA 
GAACCTTGCC TTGGAGCAGA AAATAGGAGT GAGAGAGTTG GTGAAAGTGA AGGGGGTGTC 
ATGGGACAAT CATCTGCTAA TGATGACATT CCAAAAGATG CAGAGGAAGT GAGAAAGCGC 
AAGCACCTTC AGAAAGCGGG TATCAAGGTC TTGCCCGCAA CAAAGCGCTA CAGCAGTCCA 
AGTCAATTTG CTGAAGACAT TGATGGCGCT CCAGACATTG TGCTGCGAAA AGAGAACACC 
TTCCTGAAGT CATATGAAGG GATGGGCTCT GATTCCGAAG AAGAAGAAGA GTGCAAAGTA 
CCTGATATAC AGCGAGATGA CTTGGCCACA AGGCGTGCAA GGATGAACCA ACCAAAAAGC 
CCAATGCAGT TTAATCAGTA TATACAGGGG TCATACTTAA ACAAAAACAG AGCATCAAGA 
AAGCTGAACA GTTCTAAGAC TTCCACTCCA GCTTCTCCAA AAATGTCTCA AGATATCAGG 
GAGTACCAAG GCCCCCAGTT ATTAAAGCCA TCACACGTCT GCAAAGAAAA GACATTTCCA 
ATACCCTCAG ACAATAAAGA GGATGCAGAA GCAGAGCAAG AAAAAGTTCC CGACATTCAG 
AAAGACGACC TGGCGAAACG CAAAGCTAAA GCTTCAGTTT CACTGCCAAA GGAACCATCG 
GCCTTTGCAG CGGTTTCAAT TACAAAGGCC GACATGGAAA CTTGGCAGAG GCTTCGAATG 
TCTTCTGAAG ATAGTGAGCA CTTGAATGAC AGGCAGGCTG ACCAGCAAGA GCAAACATCC 
AAGATTGATG TGACTAGAGC GACAAATGAT GACTTTGGGA GGAGGAAATT GTCTCTGCAA 
GAGAAAAGGG CTCGAAACAA ACAGAAGTTT GTACGCTTTG GCCCAGTGAC TGAAATTGAT 
CAGCAGAAGT GGGATAGGCT TTGTATAGCC AAGGCTGAAT CTGGCCAGGA AAGTGAAGAG 
ATGAAGGGCA GCATTACGTC TGATGAAACT GCCAGCCACA TATCTCCCTC AGCTTCTGCT 
CAGCGCTCCC AACCCCAGGA GGATGTTGGC ACAGGGCCAG TCTTGCCACA TGAGTCAAGA 
TCTTTTACAC CAGAGAGATT GGTTTCACCT GCTCCTCCTG AAAATGAAAG TGAAATTCAG 
CTCCTAGAAA CACAGCAAGA TGACATGATG GCAAGGAGAA CAGGTACATT CCAAAAATGC 
GCCAGAACCA GCAGAGACTC TCCTGTGTCC CACCAATATG CACTCCATAG CCAACAACAC 
GAAGAGAAGT CTGACTCGCT GGAACTCGAA GAGAAACAAG GACTCTTTCT GGATGTTAAC 
CCACAACTGA GAAAATTACT GCTGTCGGAC TTGGCAAATA CTAGTTCAAA AGCAACTAGC 
CCTACTTTGG GGAGCAAGTC TCTGAAAGTC ACCAAAGAAG CTGAAGATGT AAAAAATGAC 
ATGAAAGAAG GTGCCTTGAA CACAGGAAAG GATCCAAACT CTGTTATCCC AGAGGATAAC 
ACAATTGCAC CAGACCCAGA AAAAGATGAC ATGATGACAA GAAAAATCTC AGTGAGCCAG 
AAGCCATCTT GCGTCAGCCT TGGACACTTC CTTCCTGTTC CTTTTTCTTT GCAGCGCAGA 
GAGGGGGAAA AGAAACCAAC AAGCTGCATC ATATCAAGCA CCAAAAAGTC CAAAAATGCA 
TCCAAAGCTG TTCCAGCCGA CATTTCTGAT GTTCCTTCTA AGCCCCTTGA CCAGAAAGAA 
ACAAAAGAAC ATGGTGAATT AGGAGATACG ACTTGCAAGC AAGCCTCCAA TAGTGTGGAG 
ACTCCCATGT GTACAGAGTT AGACAGCCAG CAACAGGATG GTCTTGAGAG GAGAATGATA 
CTGTCTACTG ACTTGGCCAA TACTGAGTTA AAAATAACTC CTCCTACTTT GGCCAACAAC 
TCTCCAACCA TAACAAAAGT ACAAGAGCCA GATGCAACCA TCTCAGAGGA CAATACAACT 
GCACCAAACC CTGAAAAAGA TGATATGATG ACCAGAAGAA TCTCTGTAAC CCAGAAACCA 
TCGTGTGTAG GTCTTGGCCA CTTCCTTCCA GTTCCATTTT CTTTGCAGCA AAAAGTTGAG 
GAAAAGAAAT CAACGAGCTC CACTCTATCG ATCAAAATAA AGTCAGATAA TTGCACCCAA 
GCACTTGCAG TCGGCAGTTC TGACCTTCCT TGTAAGCTCC TCGGCCAATG TGAAACAGAG 
GGACATGTTG AATTACGATC CGATGTGACT TGCGATCAAA CCTCCACTGA GATCATTAGT 
GATGGGATTC CCACTGGTAA AGGGTTAGAT ACCCAGGCAG AAGACGCTCT TGAGACATGT 
GACCATGAGT TATTCAGATT CAGCCCAAGA TCTGTTCTGT CTGATGATGG AGAGAGCATG 
AGCATGTGCG ACATGAGGTA CGAAGAAGAG GGAAGTATGC AGCCTCACAG CAGGGCACAC 
TATGAGCATC TACAGCATGT CAACAGCTTG CTCAAGGAGG AGGATGACAC ATGGCAAGAT 
GACCTAGCTA AGTGGAAGAC ACGGAGAAGA AGTGCATCAC AGGATCTGAT CAAAAAAGAG 
GAACAGAGGA AGAAGATGGA GAGAATGATG AGCGGAGAGG ATGAACTGTC GGAGCGTAGG 
AAAAGCATAA AAACATATAG AGAGATTGTG GAAGAAAAAG AACGTCGAGA GAAAGAACTG 
CATGAAGCCT ACAAAAATGC CAAGTCAAGA GATGAGGCTG AACACATATT ACAGAGCTAC 
ATTGAAAAGT TTACAATTAG TGAAGCTGTT CTTGAGGGAT TAGTAATGCC AAAAATCTTA 
GAAAGAAGCC AGTCGGTGGA GCCAAGTTTA TTGTCTTCAT CAGAGGACCC AAACATCCTG 
AAATACCTGA GGCAACAGTC TTTACCATCA CCAAAATACA CTTCCACTGT TGAAGCAACT 
GTCTCCCCCA GCAGCCCATG TGAAGCAAAA AGTCCAACAG GTCTGATATC ACCAAGCAAA 
AACATTATCT CAAAGACTGT GCCTATGCTA ACAGCCAAGC CTTACTCCCA GCCCAAAAAC 
ACCCAGGAAG TCCTCAAGAC ATTTAAGGTA GATGGAAAGG TAAACCTCAA CGGGGTAGAA 
GAGCAGAAAG AAGAGGAACA TGGAAACCAG ACATTTGCCC CATCACTCAC CCGATCTCAG 
ATGTTTGAAG GTGTAGCCAG AGTTGATGTT CCTGTTGTTG AAATAAAGCA AGACGTAACT 
TCCATAGAGC TTAATCTGGC CAGACCAAAC ACCCTGAATT CCTTACAAGA ACCCAAAAGT 
CTCTGCGAAG ACAGAAATCA CATAAGCCAG GAGGATGAGT CAGATTCTCT GGTGCAATTA 
GAAAGAGCTC ACTCCGTGGA AATATTGTCA GAAGGTAACA CCCAATCAAG CAATGCAACC 
CAGGATGTAC CTGTTGTGAG CAGTATAGAG ATTCCACCAT TGACCATCAA TGGAAAGGAC 
ACTGTGGATG AGAGGAAAGT GGAAGCTGCC GAGTTGATCA TGCATTGGTC CCTGGAGCCG 
AATCATAAGG AAGCCAAAGA AAATTCTTTA TCGCCTTTAA AAACTTCTGA AAATTTAGAA 
GAATCCTTGC AGTTTAAAGA GAATGAATCA TCACACAAAG TGCAGCTAAA CATTACGCTT 
CAGAGCCGGA ACGCCTGGAG ACGCTCCCAG TTTTTTTTGC AGCCAGTGGA TACAGAATGT 
ACAGAAAATT CACCAGCACT TGTACCCAGT TTGAATTCTC CTAACCGATT CTGCACATGG 
GACCCTGAAG AAGAACGCAA ACGGCAAGAA AAATGGCAGC AGGAGCAGGA AAGGCTTCTC 
CAGGAGAGGT ACCAGAGAGA GCAGGAAAAA CTTAAAGAAG AATGGGAAAA AGCTCAGAAA 
GAAGTAGAAG AGGAGGAAAG GAAATATTAT GAGGAGGAAA GAAAGATAAT TGAAGACACT 
GTGGTCCCAC TGACTTTGTC TCCAAATTCT AATGCACAGT TTAATATCTC TGAAAGAAAT 
GGGAACTTCA TTCCAGCACT TACAGAACAT GTCAAGAGAG ATCAAGAAAA TAACAATGAA 
AAGCTCTTGA CAGAGTCCAG CAGAACGATA AAGAATGAGA CACATATGCA CAGCAATGTA 
ACACAGCAAC TGCTTTTGCA TACACAGGAG GGGTCAATTC TTGGTGCTAA AGAGAAACAA 
ATAAAGGGAA AAACAGACAA GTGGGAAGCA GAACAGTCCA TCTGTATTAT GGACCGAGAG 
GAGCTGAGCA ACCATGTGCA GTCTAATGAA TGTAATACAC CCACTAGGTT ACCCTTGCAG 
GCAGGATGTA AACAAGTGAC GTCATCAGAG TCAAGTCTGC CTGGGGATCT TGGATCACCA 
AAAAATCCTA TCACTGAAAA AGATGCTATT CAGAATATGT CGTCCATAAT ACCCACACGG 
GGAACTGATT TCAAGGAAGA TACAATGAAT TCTTCACAGT CCTCTACATC ACAGTGCCAG 
TCACCAAACA GGTCTGTTAG TGGGAAAAAA CTGTGCTCCA CATGTGGACT TCCTCTAGGC 
AAGGGAGCTG CCATGATAAT TGAAACCCTC AGTCTTTATT TCCACATCCA GTGCTTCAAG 
TGTGGACTGT GTAAGGGACA ACTTGGGGAT GCGATCACCG GCACAGACGT CAGAATTCGA 
AATGGCCTAC TGAACTGCAA TGACTGTTAC ATGAAGTCTA GAACGGCTGG CCACCCCACC 
CCTTTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004911275.2
CDS	491..6259
Translation	MASSATLQGEEIPPPQESPPDTACREAQKWIEQVTGKSFGDKDFRTGLENGILLCELLNAIKPGLVKKINRLPTPIAGLDNITLFLRGCKELGLKESQLFDPGDLQDTANRTTARNSDCNRKLRNVLITVYWLGKAASSCSSYSGNNLDLKEFEGLLAQMRKENEEIESPKRSIRDSGYIDCWDSERSDSLSPPRHGRDDSFDSLDSFGSRSQQTPSPDVVLRGSSDGRGSDSESDLPHRRLPDVRKDDMSARRVSYGENKAFVPFNQYLPNKSNQTSYIPAPLRKKKAEREDYRKSWSTATSPLGGERPFSPKQSPVIKEEQNELQCEGEGTKQMIALKEPCLGAENRSERVGESEGGVMGQSSANDDIPKDAEEVRKRKHLQKAGIKVLPATKRYSSPSQFAEDIDGAPDIVLRKENTFLKSYEGMGSDSEEEEECKVPDIQRDDLATRRARMNQPKSPMQFNQYIQGSYLNKNRASRKLNSSKTSTPASPKMSQDIREYQGPQLLKPSHVCKEKTFPIPSDNKEDAEAEQEKVPDIQKDDLAKRKAKASVSLPKEPSAFAAVSITKADMETWQRLRMSSEDSEHLNDRQADQQEQTSKIDVTRATNDDFGRRKLSLQEKRARNKQKFVRFGPVTEIDQQKWDRLCIAKAESGQESEEMKGSITSDETASHISPSASAQRSQPQEDVGTGPVLPHESRSFTPERLVSPAPPENESEIQLLETQQDDMMARRTGTFQKCARTSRDSPVSHQYALHSQQHEEKSDSLELEEKQGLFLDVNPQLRKLLLSDLANTSSKATSPTLGSKSLKVTKEAEDVKNDMKEGALNTGKDPNSVIPEDNTIAPDPEKDDMMTRKISVSQKPSCVSLGHFLPVPFSLQRREGEKKPTSCIISSTKKSKNASKAVPADISDVPSKPLDQKETKEHGELGDTTCKQASNSVETPMCTELDSQQQDGLERRMILSTDLANTELKITPPTLANNSPTITKVQEPDATISEDNTTAPNPEKDDMMTRRISVTQKPSCVGLGHFLPVPFSLQQKVEEKKSTSSTLSIKIKSDNCTQALAVGSSDLPCKLLGQCETEGHVELRSDVTCDQTSTEIISDGIPTGKGLDTQAEDALETCDHELFRFSPRSVLSDDGESMSMCDMRYEEEGSMQPHSRAHYEHLQHVNSLLKEEDDTWQDDLAKWKTRRRSASQDLIKKEEQRKKMERMMSGEDELSERRKSIKTYREIVEEKERREKELHEAYKNAKSRDEAEHILQSYIEKFTISEAVLEGLVMPKILERSQSVEPSLLSSSEDPNILKYLRQQSLPSPKYTSTVEATVSPSSPCEAKSPTGLISPSKNIISKTVPMLTAKPYSQPKNTQEVLKTFKVDGKVNLNGVEEQKEEEHGNQTFAPSLTRSQMFEGVARVDVPVVEIKQDVTSIELNLARPNTLNSLQEPKSLCEDRNHISQEDESDSLVQLERAHSVEILSEGNTQSSNATQDVPVVSSIEIPPLTINGKDTVDERKVEAAELIMHWSLEPNHKEAKENSLSPLKTSENLEESLQFKENESSHKVQLNITLQSRNAWRRSQFFLQPVDTECTENSPALVPSLNSPNRFCTWDPEEERKRQEKWQQEQERLLQERYQREQEKLKEEWEKAQKEVEEEERKYYEEERKIIEDTVVPLTLSPNSNAQFNISERNGNFIPALTEHVKRDQENNNEKLLTESSRTIKNETHMHSNVTQQLLLHTQEGSILGAKEKQIKGKTDKWEAEQSICIMDREELSNHVQSNECNTPTRLPLQAGCKQVTSSESSLPGDLGSPKNPITEKDAIQNMSSIIPTRGTDFKEDTMNSSQSSTSQCQSPNRSVSGKKLCSTCGLPLGKGAAMIIETLSLYFHIQCFKCGLCKGQLGDAITGTDVRIRNGLLNCNDCYMKSRTAGHPTPL

Target ORF information:

RefSeq Version	XM_004911218.4
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis LIM and calponin homology domains 1 (limch1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004911218.4

ORF Insert Sequence:

ATGGCCAGCT CCGCCACACT GCAAGGGGAA GAGATCCCCC CGCCCCAGGA GTCTCCCCCA 
GACACGGCTT GCAGGGAGGC ACAGAAGTGG ATTGAGCAAG TTACTGGCAA AAGCTTTGGC 
GACAAAGACT TCAGGACTGG ACTGGAGAAT GGAATCCTTC TGTGCGAGTT ATTAAATGCT 
ATTAAACCAG GACTCGTAAA AAAGATCAAC AGACTTCCTA CTCCCATTGC TGGATTGGAC 
AATATAACAT TATTCCTTAG AGGCTGCAAA GAGCTCGGGC TGAAAGAATC TCAGTTGTTT 
GACCCAGGTG ACCTACAGGA CACAGCAAAC AGGACAACAG CCAGGAATTC AGATTGCAAC 
AGAAAACTAA GAAATGTACT AATCACCGTG TACTGGCTGG GGAAAGCTGC AAGCAGCTGC 
TCGTCCTATA GTGGCAACAA CCTGGACTTA AAGGAGTTTG AGGGATTGCT GGCACAAATG 
AGAAAAGAAA ATGAGGAAAT AGAGAGTCCA AAACGCAGCA TACGGGACAG TGGCTACATA 
GATTGTTGGG ACTCTGAGAG AAGTGACTCC CTTTCTCCTC CCAGACATGG TAGAGATGAC 
TCCTTCGATA GTTTAGACTC ATTTGGATCT AGATCACAAC AGACACCATC TCCAGATGTA 
GTGCTGAGGG GAAGCAGCGA CGGCAGAGGA AGCGACTCTG AGTCTGACCT ACCTCACAGA 
AGGTTACCTG ATGTGCGAAA AGATGATATG TCTGCTAGAC GGGTATCCTA CGGTGAAAAC 
AAAGCATTTG TGCCTTTCAA CCAATACCTC CCCAACAAGA GCAACCAGAC CTCCTACATC 
CCGGCACCCC TGAGGAAGAA GAAGGCTGAG CGTGAGGACT ACCGGAAAAG CTGGAGCACA 
GCTACATCCC CCCTGGGGGG AGAGCGACCC TTCAGTCCCA AACAAAGTCC AGTCATTAAA 
GAAGAACAAA ACGAGTTGCA GTGTGAAGGA GAAGGGACCA AGCAGATGAT TGCCTTAAAA 
GAACCTTGCC TTGGAGCAGA AAATAGGAGT GAGAGAGTTG GTGAAAGTGA AGGGGGTGTC 
ATGGGACAAT CATCTGCTAA TGATGACATT CCAAAAGATG CAGAGGAAGT GAGAAAGCGC 
AAGCACCTTC AGAAAGCGGG TATCAAGGTC TTGCCCGCAA CAAAGCGCTA CAGCAGTCCA 
AGTCAATTTG CTGAAGACAT TGATGGCGCT CCAGACATTG TGCTGCGAAA AGAGAACACC 
TTCCTGAAGT CATATGAAGG GATGGGCTCT GATTCCGAAG AAGAAGAAGA GTGCAAAGTA 
CCTGATATAC AGCGAGATGA CTTGGCCACA AGGCGTGCAA GGATGAACCA ACCAAAAAGC 
CCAATGCAGT TTAATCAGTA TATACAGGGG TCATACTTAA ACAAAAACAG AGCATCAAGA 
AAGCTGAACA GTTCTAAGAC TTCCACTCCA GCTTCTCCAA AAATGTCTCA AGATATCAGG 
GAGTACCAAG GCCCCCAGTT ATTAAAGCCA TCACACGTCT GCAAAGAAAA GACATTTCCA 
ATACCCTCAG ACAATAAAGA GGATGCAGAA GCAGAGCAAG AAAAAGTTCC CGACATTCAG 
AAAGACGACC TGGCGAAACG CAAAGCTAAA GCTTCAGTTT CACTGCCAAA GGAACCATCG 
GCCTTTGCAG CGGTTTCAAT TACAAAGGCC GACATGGAAA CTTGGCAGAG GCTTCGAATG 
TCTTCTGAAG ATAGTGAGCA CTTGAATGAC AGGCAGGCTG ACCAGCAAGA GCAAACATCC 
AAGATTGATG TGACTAGAGC GACAAATGAT GACTTTGGGA GGAGGAAATT GTCTCTGCAA 
GAGAAAAGGG CTCGAAACAA ACAGAAGTTT GTACGCTTTG GCCCAGTGAC TGAAATTGAT 
CAGCAGAAGT GGGATAGGCT TTGTATAGCC AAGGCTGAAT CTGGCCAGGA AAGTGAAGAG 
ATGAAGGGCA GCATTACGTC TGATGAAACT GCCAGCCACA TATCTCCCTC AGCTTCTGCT 
CAGCGCTCCC AACCCCAGGA GGATGTTGGC ACAGGGCCAG TCTTGCCACA TGAGTCAAGA 
TCTTTTACAC CAGAGAGATT GGTTTCACCT GCTCCTCCTG AAAATGAAAG TGAAATTCAG 
CTCCTAGAAA CACAGCAAGA TGACATGATG GCAAGGAGAA CAGGTACATT CCAAAAATGC 
GCCAGAACCA GCAGAGACTC TCCTGTGTCC CACCAATATG CACTCCATAG CCAACAACAC 
GAAGAGAAGT CTGACTCGCT GGAACTCGAA GAGAAACAAG GACTCTTTCT GGATGTTAAC 
CCACAACTGA GAAAATTACT GCTGTCGGAC TTGGCAAATA CTAGTTCAAA AGCAACTAGC 
CCTACTTTGG GGAGCAAGTC TCTGAAAGTC ACCAAAGAAG CTGAAGATGT AAAAAATGAC 
ATGAAAGAAG GTGCCTTGAA CACAGGAAAG GATCCAAACT CTGTTATCCC AGAGGATAAC 
ACAATTGCAC CAGACCCAGA AAAAGATGAC ATGATGACAA GAAAAATCTC AGTGAGCCAG 
AAGCCATCTT GCGTCAGCCT TGGACACTTC CTTCCTGTTC CTTTTTCTTT GCAGCGCAGA 
GAGGGGGAAA AGAAACCAAC AAGCTGCATC ATATCAAGCA CCAAAAAGTC CAAAAATGCA 
TCCAAAGCTG TTCCAGCCGA CATTTCTGAT GTTCCTTCTA AGCCCCTTGA CCAGAAAGAA 
ACAAAAGAAC ATGGTGAATT AGGAGATACG ACTTGCAAGC AAGCCTCCAA TAGTGTGGAG 
ACTCCCATGT GTACAGAGTT AGACAGCCAG CAACAGGATG GTCTTGAGAG GAGAATGATA 
CTGTCTACTG ACTTGGCCAA TACTGAGTTA AAAATAACTC CTCCTACTTT GGCCAACAAC 
TCTCCAACCA TAACAAAAGT ACAAGAGCCA GATGCAACCA TCTCAGAGGA CAATACAACT 
GCACCAAACC CTGAAAAAGA TGATATGATG ACCAGAAGAA TCTCTGTAAC CCAGAAACCA 
TCGTGTGTAG GTCTTGGCCA CTTCCTTCCA GTTCCATTTT CTTTGCAGCA AAAAGTTGAG 
GAAAAGAAAT CAACGAGCTC CACTCTATCG ATCAAAATAA AGTCAGATAA TTGCACCCAA 
GCACTTGCAG TCGGCAGTTC TGACCTTCCT TGTAAGCTCC TCGGCCAATG TGAAACAGAG 
GGACATGTTG AATTACGATC CGATGTGACT TGCGATCAAA CCTCCACTGA GATCATTAGT 
GATGGGATTC CCACTGGTAA AGGGTTAGAT ACCCAGGCAG AAGACGCTCT TGAGACATGT 
GACCATGAGT TATTCAGATT CAGCCCAAGA TCTGTTCTGT CTGATGATGG AGAGAGCATG 
AGCATGTGCG ACATGAGGTA CGAAGAAGAG GGAAGTATGC AGCCTCACAG CAGGGCACAC 
TATGAGCATC TACAGCATGT CAACAGCTTG CTCAAGGAGG AGGATGACAC ATGGCAAGAT 
GACCTAGCTA AGTGGAAGAC ACGGAGAAGA AGTGCATCAC AGGATCTGAT CAAAAAAGAG 
GAACAGAGGA AGAAGATGGA GAGAATGATG AGCGGAGAGG ATGAACTGTC GGAGCGTAGG 
AAAAGCATAA AAACATATAG AGAGATTGTG GAAGAAAAAG AACGTCGAGA GAAAGAACTG 
CATGAAGCCT ACAAAAATGC CAAGTCAAGA GATGAGGCTG AACACATATT ACAGAGCTAC 
ATTGAAAAGT TTACAATTAG TGAAGCTGTT CTTGAGGGAT TAGTAATGCC AAAAATCTTA 
GAAAGAAGCC AGTCGGTGGA GCCAAGTTTA TTGTCTTCAT CAGAGGACCC AAACATCCTG 
AAATACCTGA GGCAACAGTC TTTACCATCA CCAAAATACA CTTCCACTGT TGAAGCAACT 
GTCTCCCCCA GCAGCCCATG TGAAGCAAAA AGTCCAACAG GTCTGATATC ACCAAGCAAA 
AACATTATCT CAAAGACTGT GCCTATGCTA ACAGCCAAGC CTTACTCCCA GCCCAAAAAC 
ACCCAGGAAG TCCTCAAGAC ATTTAAGGTA GATGGAAAGG TAAACCTCAA CGGGGTAGAA 
GAGCAGAAAG AAGAGGAACA TGGAAACCAG ACATTTGCCC CATCACTCAC CCGATCTCAG 
ATGTTTGAAG GTGTAGCCAG AGTTGATGTT CCTGTTGTTG AAATAAAGCA AGACGTAACT 
TCCATAGAGC TTAATCTGGC CAGACCAAAC ACCCTGAATT CCTTACAAGA ACCCAAAAGT 
CTCTGCGAAG ACAGAAATCA CATAAGCCAG GAGGATGAGT CAGATTCTCT GGTGCAATTA 
GAAAGAGCTC ACTCCGTGGA AATATTGTCA GAAGGTAACA CCCAATCAAG CAATGCAACC 
CAGGATGTAC CTGTTGTGAG CAGTATAGAG ATTCCACCAT TGACCATCAA TGGAAAGGAC 
ACTGTGGATG AGAGGAAAGT GGAAGCTGCC GAGTTGATCA TGCATTGGTC CCTGGAGCCG 
AATCATAAGG AAGCCAAAGA AAATTCTTTA TCGCCTTTAA AAACTTCTGA AAATTTAGAA 
GAATCCTTGC AGTTTAAAGA GAATGAATCA TCACACAAAG TGCAGCTAAA CATTACGCTT 
CAGAGCCGGA ACGCCTGGAG ACGCTCCCAG TTTTTTTTGC AGCCAGTGGA TACAGAATGT 
ACAGAAAATT CACCAGCACT TGTACCCAGT TTGAATTCTC CTAACCGATT CTGCACATGG 
GACCCTGAAG AAGAACGCAA ACGGCAAGAA AAATGGCAGC AGGAGCAGGA AAGGCTTCTC 
CAGGAGAGGT ACCAGAGAGA GCAGGAAAAA CTTAAAGAAG AATGGGAAAA AGCTCAGAAA 
GAAGTAGAAG AGGAGGAAAG GAAATATTAT GAGGAGGAAA GAAAGATAAT TGAAGACACT 
GTGGTCCCAC TGACTTTGTC TCCAAATTCT AATGCACAGT TTAATATCTC TGAAAGAAAT 
GGGAACTTCA TTCCAGCACT TACAGAACAT GTCAAGAGAG ATCAAGAAAA TAACAATGAA 
AAGCTCTTGA CAGAGTCCAG CAGAACGATA AAGAATGAGA CACATATGCA CAGCAATGTA 
ACACAGCAAC TGCTTTTGCA TACACAGGAG GGGTCAATTC TTGGTGCTAA AGAGAAACAA 
ATAAAGGGAA AAACAGACAA GTGGGAAGCA GAACAGTCCA TCTGTATTAT GGACCGAGAG 
GAGCTGAGCA ACCATGTGCA GTCTAATGAA TGTAATACAC CCACTAGGTT ACCCTTGCAG 
GCAGGATGTA AACAAGTGAC GTCATCAGAG TCAAGTCTGC CTGGGGATCT TGGATCACCA 
AAAAATCCTA TCACTGAAAA AGATGCTATT CAGAATATGT CGTCCATAAT ACCCACACGG 
GGAACTGATT TCAAGGAAGA TACAATGAAT TCTTCACAGT CCTCTACATC ACAGTGCCAG 
TCACCAAACA GGTCTGTTAG TGGGAAAAAA CTGTGCTCCA CATGTGGACT TCCTCTAGGC 
AAGGGAGCTG CCATGATAAT TGAAACCCTC AGTCTTTATT TCCACATCCA GTGCTTCAAG 
TGTGGACTGT GTAAGGGACA ACTTGGGGAT GCGATCACCG GCACAGACGT CAGAATTCGA 
AATGGCCTAC TGAACTGCAA TGACTGTTAC ATGAAGTCTA GAACGGCTGG CCACCCCACC 
CCTTTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

limch1 ( XM_012955696.3 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012811150.2 Xenopus tropicalis LIM and calponin homology domains 1 (limch1), transcript variant X3, mRNA.

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CloneID	OXa49697
Clone ID Related Accession (Same CDS sequence)	XM_012955696.3
Accession Version	XM_012955696.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5694bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1576512000000
Organism	Xenopus tropicalis(tropical clawed frog)
Product	LIM and calponin homology domains-containing protein 1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012955696.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCAGCT CCGCCACACT GCAAGGGGAA GAGATCCCCC CGCCCCAGGA GTCTCCCCCA 
GACACGGCTT GCAGGGAGGC ACAGAAGTGG ATTGAGCAAG TTACTGGCAA AAGCTTTGGC 
GACAAAGACT TCAGGACTGG ACTGGAGAAT GGAATCCTTC TGTGCGAGTT ATTAAATGCT 
ATTAAACCAG GACTCGTAAA AAAGATCAAC AGACTTCCTA CTCCCATTGC TGGATTGGAC 
AATATAACAT TATTCCTTAG AGGCTGCAAA GAGCTCGGGC TGAAAGAATC TCAGTTGTTT 
GACCCAGGTG ACCTACAGGA CACAGCAAAC AGGACAACAG CCAGGAATTC AGATTGCAAC 
AGAAAACTAA GAAATGTACT AATCACCGTG TACTGGCTGG GGAAAGCTGC AAGCAGCTGC 
TCGTCCTATA GTGGCAACAA CCTGGACTTA AAGGAGTTTG AGGGATTGCT GGCACAAATG 
AGAAAAGAAA ATGAGGAAAT AGAGAGTCCA AAACGCAGCA TACGGGACAG TGGCTACATA 
GATTGTTGGG ACTCTGAGAG AAGTGACTCC CTTTCTCCTC CCAGACATGG TAGAGATGAC 
TCCTTCGATA GTTTAGACTC ATTTGGATCT AGATCACAAC AGACACCATC TCCAGATGTA 
GTGCTGAGGG GAAGCAGCGA CGGCAGAGGA AGCGACTCTG AGTCTGACCT ACCTCACAGA 
AGGTTACCTG ATGTGCGAAA AGATGATATG TCTGCTAGAC GGGTATCCTA CGGTGAAAAC 
AAAGCATTTG TGCCTTTCAA CCAATACCTC CCCAACAAGA GCAACCAGAC CTCCTACATC 
CCGGCACCCC TGAGGAAGAA GAAGGCTGAG CGTGAGGACT ACCGGAAAAG CTGGAGCACA 
GCTACATCCC CCCTGGGGGG AGAGCGACCC TTCAGTCCCA AACAAAGTCC AGTCATTAAA 
GAAGAACAAA ACGAGTTGCA GTGTGAAGGA GAAGGGACCA AGCAGATGAT TGCCTTAAAA 
GAACCTTGCC TTGGAGCAGA AAATAGGAGT GAGAGAGTTG GTGAAAGTGA AGGGGGTGTC 
ATGGGACAAT CATCTGCTAA TGATGACATT CCAAAAGATG CAGAGGAAGT GAGAAAGCGC 
AAGCACCTTC AGAAAGCGGG TATCAAGGTC TTGCCCGCAA CAAAGCGCTA CAGCAGTCCA 
AGTCAATTTG CTGAAGACAT TGATGGCGCT CCAGACATTG TGCTGCGAAA AGAGAACACC 
TTCCTGAAGT CATATGAAGG GATGGGCTCT GATTCCGAAG AAGAAGAAGA GTGCAAAGTA 
CCTGATATAC AGCGAGATGA CTTGGCCACA AGGCGTGCAA GGATGAACCA ACCAAAAAGC 
CCAATGCAGT TTAATCAGTA TATACAGGGG TCATACTTAA ACAAAAACAG AGCATCAAGA 
AAGCTGAACA GTTCTAAGAC TTCCACTCCA GCTTCTCCAA AAATGTCTCA AGATATCAGG 
GAGTACCAAG GCCCCCAGTT ATTAAAGCCA TCACACGTCT GCAAAGAAAA GACATTTCCA 
ATACCCTCAG ACAATAAAGA GGATGCAGAA GCAGAGCAAG AAAAAGTTCC CGACATTCAG 
AAAGACGACC TGGCGAAACG CAAAGCTAAA GCTTCAGTTT CACTGCCAAA GGAACCATCG 
GCCTTTGCAG CGGTTTCAAT TACAAAGGCC GACATGGAAA CTTGGCAGAG GCTTCGAATG 
TCTTCTGAAG ATAGTGAGCA CTTGAATGAC AGGCAGGCTG ACCAGCAAGA GCAAACATCC 
AAGATTGATG TGACTAGAGC GACAAATGAT GACTTTGGGA GGAGGAAATT GTCTCTGCAA 
GAGAAAAGGG CTCGAAACAA ACAGAAGTTT GTACGCTTTG GCCCAGTGAC TGAAATTGAT 
CAGCAGAAGT GGGATAGGCT TTGTATAGCC AAGGCTGAAT CTGGCCAGGA AAGTGAAGAG 
ATGAAGGGCA GCATTACGTC TGATGAAACT GCCAGCCACA TATCTCCCTC AGCTTCTGCT 
CAGCGCTCCC AACCCCAGGA GGATGTTGGC ACAGGGCCAG TCTTGCCACA TGAGTCAAGA 
TCTTTTACAC CAGAGAGATT GGTTTCACCT GCTCCTCCTG AAAATGAAAG TGAAATTCAG 
CTCCTAGAAA CACAGCAAGA TGACATGATG GCAAGGAGAA CAGGTACATT CCAAAAATGC 
GCCAGAACCA GCAGAGACTC TCCTGTGTCC CACCAATATG CACTCCATAG CCAACAACAC 
GAAGAGAAGT CTGACTCGCT GGAACTCGAA GAGAAACAAG GACTCTTTCT GGATGTTAAC 
CCACAACTGA GAAAATTACT GCTGTCGGAC TTGGCAAATA CTAGTTCAAA AGCAACTAGC 
CCTACTTTGG GGAGCAAGTC TCTGAAAGTC ACCAAAGAAG CTGAAGATGT AAAAAATGAC 
ATGAAAGAAG GTGCCTTGAA CACAGGAAAG GATCCAAACT CTGTTATCCC AGAGGATAAC 
ACAATTGCAC CAGACCCAGA AAAAGATGAC ATGATGACAA GAAAAATCTC AGTGAGCCAG 
AAGCCATCTT GCGTCAGCCT TGGACACTTC CTTCCTGTTC CTTTTTCTTT GCAGCGCAGA 
GAGGGGGAAA AGAAACCAAC AAGCTGCATC ATATCAAGCA CCAAAAAGTC CAAAAATGCA 
TCCAAAGCTG TTCCAGCCGA CATTTCTGAT GTTCCTTCTA AGCCCCTTGA CCAGAAAGAA 
ACAAAAGAAC ATGGTGAATT AGGAGATACG ACTTGCAAGC AAGCCTCCAA TAGTGTGGAG 
ACTCCCATGT GTACAGAGTT AGACAGCCAG CAACAGGATG GTCTTGAGAG GAGAATGATA 
CTGTCTACTG ACTTGGCCAA TACTGAGTTA AAAATAACTC CTCCTACTTT GGCCAACAAC 
TCTCCAACCA TAACAAAAGT ACAAGAGCCA GATGCAACCA TCTCAGAGGA CAATACAACT 
GCACCAAACC CTGAAAAAGA TGATATGATG ACCAGAAGAA TCTCTGTAAC CCAGAAACCA 
TCGTGTGTAG GTCTTGGCCA CTTCCTTCCA GTTCCATTTT CTTTGCAGCA AAAAGTTGAG 
GAAAAGAAAT CAACGAGCTC CACTCTATCG ATCAAAATAA AGTCAGATAA TTGCACCCAA 
GCACTTGCAG TCGGCAGTTC TGACCTTCCT TGTAAGCTCC TCGGCCAATG TGAAACAGAG 
GGACATGTTG AATTACGATC CGATGTGACT TGCGATCAAA CCTCCACTGA GATCATTAGT 
GATGGGATTC CCACTGGTAA AGGGTTAGAT ACCCAGGCAG AAGACGCTCT TGAGACATGT 
GACCATGAGT TATTCAGATT CAGCCCAAGA TCTGTTCTGT CTGATGATGG AGAGAGCATG 
AGCATGTGCG ACATGAGGTA CGAAGAAGAG GGAAGTATGC AGCCTCACAG CAGGGCACAC 
TATGAGCATC TACAGCATGT CAACAGCTTG CTCAAGGAGG AGGATGACAC ATGGCAAGAT 
GACCTAGCTA AGTGGAAGAC ACGGAGAAGA AGTGCATCAC AGGATCTGAT CAAAAAAGAG 
GAACAGAGGA AGAAGATGGA GAGAATGATG AGCGGAGAGG ATGAACTGTC GGAGCGTAGG 
AAAAGCATAA AAACATATAG AGAGATTGTG GAAGAAAAAG AACGTCGAGA GAAAGAACTG 
CATGAAGCCT ACAAAAATGC CAAGTCAAGA GATGAGGCTG AACACATATT ACAGAGCTAC 
ATTGAAAAGT TTACAATTAG TGAAGCTGTT CTTGAGGGAT TAGTAATGCC AAAAATCTTA 
GAAAGAAGCC AGTCGGTGGA GCCAAGTTTA TTGTCTTCAT CAGAGGACCC AAACATCCTG 
AAATACCTGA GGCAACAGTC TTTACCATCA CCAAAATACA CTTCCACTGT TGAAGCAACT 
GTCTCCCCCA GCAGCCCATG TGAAGCAAAA AGTCCAACAG GTCTGATATC ACCAAGCAAA 
AACATTATCT CAAAGACTGT GCCTATGCTA ACAGCCAAGC CTTACTCCCA GCCCAAAAAC 
ACCCAGGAAG TCCTCAAGAC ATTTAAGGTA GATGGAAAGG TAAACCTCAA CGGGGTAGAA 
GAGCAGAAAG AAGAGGAACA TGGAAACCAG ACATTTGCCC CATCACTCAC CCGATCTCAG 
ATGTTTGAAG GTGTAGCCAG AGTTGATGTT CCTGTTGTTG AAATAAAGCA AGACGTAACT 
TCCATAGAGC TTAATCTGGC CAGACCAAAC ACCCTGAATT CCTTACAAGA ACCCAAAAGT 
CTCTGCGAAG ACAGAAATCA CATAAGCCAG GAGGATGAGT CAGATTCTCT GGTGCAATTA 
GAAAGAGCTC ACTCCGTGGA AATATTGTCA GAAGGTAACA CCCAATCAAG CAATGCAACC 
CAGGATGTAC CTGTTGTGAG CAGTATAGAG ATTCCACCAT TGACCATCAA TGGAAAGGAC 
ACTGTGGATG AGAGGAAAGT GGAAGCTGCC GAGTTGATCA TGCATTGGTC CCTGGAGCCG 
AATCATAAGG AAGCCAAAGA AAATTCTTTA TCGCCTTTAA AAACTTCTGA AAATTTAGAA 
GAATCCTTGC AGTTTAAAGA GAATGTGGAT ACAGAATGTA CAGAAAATTC ACCAGCACTT 
GTACCCAGTT TGAATTCTCC TAACCGATTC TGCACATGGG ACCCTGAAGA AGAACGCAAA 
CGGCAAGAAA AATGGCAGCA GGAGCAGGAA AGGCTTCTCC AGGAGAGGTA CCAGAGAGAG 
CAGGAAAAAC TTAAAGAAGA ATGGGAAAAA GCTCAGAAAG AAGTAGAAGA GGAGGAAAGG 
AAATATTATG AGGAGGAAAG AAAGATAATT GAAGACACTG TGGTCCCACT GACTTTGTCT 
CCAAATTCTA ATGCACAGTT TAATATCTCT GAAAGAAATG GGAACTTCAT TCCAGCACTT 
ACAGAACATG TCAAGAGAGA TCAAGAAAAT AACAATGAAA AGCTCTTGAC AGAGTCCAGC 
AGAACGATAA AGAATGAGAC ACATATGCAC AGCAATGTAA CACAGCAACT GCTTTTGCAT 
ACACAGGAGG GGTCAATTCT TGGTGCTAAA GAGAAACAAA TAAAGGGAAA AACAGACAAG 
TGGGAAGCAG AACAGTCCAT CTGTATTATG GACCGAGAGG AGCTGAGCAA CCATGTGCAG 
TCTAATGAAT GTAATACACC CACTAGGTTA CCCTTGCAGG CAGGATGTAA ACAAGTGACG 
TCATCAGAGT CAAGTCTGCC TGGGGATCTT GGATCACCAA AAAATCCTAT CACTGAAAAA 
GATGCTATTC AGAATATGTC GTCCATAATA CCCACACGGG GAACTGATTT CAAGGAAGAT 
ACAATGAATT CTTCACAGTC CTCTACATCA CAGTGCCAGT CACCAAACAG GTCTGTTAGT 
GGGAAAAAAC TGTGCTCCAC ATGTGGACTT CCTCTAGGCA AGGGAGCTGC CATGATAATT 
GAAACCCTCA GTCTTTATTT CCACATCCAG TGCTTCAAGT GTGGACTGTG TAAGGGACAA 
CTTGGGGATG CGATCACCGG CACAGACGTC AGAATTCGAA ATGGCCTACT GAACTGCAAT 
GACTGTTACA TGAAGTCTAG AAGTAAGGCG GCTGGCCACC CCACCCCTTT GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012811150.2
CDS	491..6184
Translation	MASSATLQGEEIPPPQESPPDTACREAQKWIEQVTGKSFGDKDFRTGLENGILLCELLNAIKPGLVKKINRLPTPIAGLDNITLFLRGCKELGLKESQLFDPGDLQDTANRTTARNSDCNRKLRNVLITVYWLGKAASSCSSYSGNNLDLKEFEGLLAQMRKENEEIESPKRSIRDSGYIDCWDSERSDSLSPPRHGRDDSFDSLDSFGSRSQQTPSPDVVLRGSSDGRGSDSESDLPHRRLPDVRKDDMSARRVSYGENKAFVPFNQYLPNKSNQTSYIPAPLRKKKAEREDYRKSWSTATSPLGGERPFSPKQSPVIKEEQNELQCEGEGTKQMIALKEPCLGAENRSERVGESEGGVMGQSSANDDIPKDAEEVRKRKHLQKAGIKVLPATKRYSSPSQFAEDIDGAPDIVLRKENTFLKSYEGMGSDSEEEEECKVPDIQRDDLATRRARMNQPKSPMQFNQYIQGSYLNKNRASRKLNSSKTSTPASPKMSQDIREYQGPQLLKPSHVCKEKTFPIPSDNKEDAEAEQEKVPDIQKDDLAKRKAKASVSLPKEPSAFAAVSITKADMETWQRLRMSSEDSEHLNDRQADQQEQTSKIDVTRATNDDFGRRKLSLQEKRARNKQKFVRFGPVTEIDQQKWDRLCIAKAESGQESEEMKGSITSDETASHISPSASAQRSQPQEDVGTGPVLPHESRSFTPERLVSPAPPENESEIQLLETQQDDMMARRTGTFQKCARTSRDSPVSHQYALHSQQHEEKSDSLELEEKQGLFLDVNPQLRKLLLSDLANTSSKATSPTLGSKSLKVTKEAEDVKNDMKEGALNTGKDPNSVIPEDNTIAPDPEKDDMMTRKISVSQKPSCVSLGHFLPVPFSLQRREGEKKPTSCIISSTKKSKNASKAVPADISDVPSKPLDQKETKEHGELGDTTCKQASNSVETPMCTELDSQQQDGLERRMILSTDLANTELKITPPTLANNSPTITKVQEPDATISEDNTTAPNPEKDDMMTRRISVTQKPSCVGLGHFLPVPFSLQQKVEEKKSTSSTLSIKIKSDNCTQALAVGSSDLPCKLLGQCETEGHVELRSDVTCDQTSTEIISDGIPTGKGLDTQAEDALETCDHELFRFSPRSVLSDDGESMSMCDMRYEEEGSMQPHSRAHYEHLQHVNSLLKEEDDTWQDDLAKWKTRRRSASQDLIKKEEQRKKMERMMSGEDELSERRKSIKTYREIVEEKERREKELHEAYKNAKSRDEAEHILQSYIEKFTISEAVLEGLVMPKILERSQSVEPSLLSSSEDPNILKYLRQQSLPSPKYTSTVEATVSPSSPCEAKSPTGLISPSKNIISKTVPMLTAKPYSQPKNTQEVLKTFKVDGKVNLNGVEEQKEEEHGNQTFAPSLTRSQMFEGVARVDVPVVEIKQDVTSIELNLARPNTLNSLQEPKSLCEDRNHISQEDESDSLVQLERAHSVEILSEGNTQSSNATQDVPVVSSIEIPPLTINGKDTVDERKVEAAELIMHWSLEPNHKEAKENSLSPLKTSENLEESLQFKENVDTECTENSPALVPSLNSPNRFCTWDPEEERKRQEKWQQEQERLLQERYQREQEKLKEEWEKAQKEVEEEERKYYEEERKIIEDTVVPLTLSPNSNAQFNISERNGNFIPALTEHVKRDQENNNEKLLTESSRTIKNETHMHSNVTQQLLLHTQEGSILGAKEKQIKGKTDKWEAEQSICIMDREELSNHVQSNECNTPTRLPLQAGCKQVTSSESSLPGDLGSPKNPITEKDAIQNMSSIIPTRGTDFKEDTMNSSQSSTSQCQSPNRSVSGKKLCSTCGLPLGKGAAMIIETLSLYFHIQCFKCGLCKGQLGDAITGTDVRIRNGLLNCNDCYMKSRSKAAGHPTPL

Target ORF information:

RefSeq Version	XM_012955696.3
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis LIM and calponin homology domains 1 (limch1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012955696.3

ORF Insert Sequence:

ATGGCCAGCT CCGCCACACT GCAAGGGGAA GAGATCCCCC CGCCCCAGGA GTCTCCCCCA 
GACACGGCTT GCAGGGAGGC ACAGAAGTGG ATTGAGCAAG TTACTGGCAA AAGCTTTGGC 
GACAAAGACT TCAGGACTGG ACTGGAGAAT GGAATCCTTC TGTGCGAGTT ATTAAATGCT 
ATTAAACCAG GACTCGTAAA AAAGATCAAC AGACTTCCTA CTCCCATTGC TGGATTGGAC 
AATATAACAT TATTCCTTAG AGGCTGCAAA GAGCTCGGGC TGAAAGAATC TCAGTTGTTT 
GACCCAGGTG ACCTACAGGA CACAGCAAAC AGGACAACAG CCAGGAATTC AGATTGCAAC 
AGAAAACTAA GAAATGTACT AATCACCGTG TACTGGCTGG GGAAAGCTGC AAGCAGCTGC 
TCGTCCTATA GTGGCAACAA CCTGGACTTA AAGGAGTTTG AGGGATTGCT GGCACAAATG 
AGAAAAGAAA ATGAGGAAAT AGAGAGTCCA AAACGCAGCA TACGGGACAG TGGCTACATA 
GATTGTTGGG ACTCTGAGAG AAGTGACTCC CTTTCTCCTC CCAGACATGG TAGAGATGAC 
TCCTTCGATA GTTTAGACTC ATTTGGATCT AGATCACAAC AGACACCATC TCCAGATGTA 
GTGCTGAGGG GAAGCAGCGA CGGCAGAGGA AGCGACTCTG AGTCTGACCT ACCTCACAGA 
AGGTTACCTG ATGTGCGAAA AGATGATATG TCTGCTAGAC GGGTATCCTA CGGTGAAAAC 
AAAGCATTTG TGCCTTTCAA CCAATACCTC CCCAACAAGA GCAACCAGAC CTCCTACATC 
CCGGCACCCC TGAGGAAGAA GAAGGCTGAG CGTGAGGACT ACCGGAAAAG CTGGAGCACA 
GCTACATCCC CCCTGGGGGG AGAGCGACCC TTCAGTCCCA AACAAAGTCC AGTCATTAAA 
GAAGAACAAA ACGAGTTGCA GTGTGAAGGA GAAGGGACCA AGCAGATGAT TGCCTTAAAA 
GAACCTTGCC TTGGAGCAGA AAATAGGAGT GAGAGAGTTG GTGAAAGTGA AGGGGGTGTC 
ATGGGACAAT CATCTGCTAA TGATGACATT CCAAAAGATG CAGAGGAAGT GAGAAAGCGC 
AAGCACCTTC AGAAAGCGGG TATCAAGGTC TTGCCCGCAA CAAAGCGCTA CAGCAGTCCA 
AGTCAATTTG CTGAAGACAT TGATGGCGCT CCAGACATTG TGCTGCGAAA AGAGAACACC 
TTCCTGAAGT CATATGAAGG GATGGGCTCT GATTCCGAAG AAGAAGAAGA GTGCAAAGTA 
CCTGATATAC AGCGAGATGA CTTGGCCACA AGGCGTGCAA GGATGAACCA ACCAAAAAGC 
CCAATGCAGT TTAATCAGTA TATACAGGGG TCATACTTAA ACAAAAACAG AGCATCAAGA 
AAGCTGAACA GTTCTAAGAC TTCCACTCCA GCTTCTCCAA AAATGTCTCA AGATATCAGG 
GAGTACCAAG GCCCCCAGTT ATTAAAGCCA TCACACGTCT GCAAAGAAAA GACATTTCCA 
ATACCCTCAG ACAATAAAGA GGATGCAGAA GCAGAGCAAG AAAAAGTTCC CGACATTCAG 
AAAGACGACC TGGCGAAACG CAAAGCTAAA GCTTCAGTTT CACTGCCAAA GGAACCATCG 
GCCTTTGCAG CGGTTTCAAT TACAAAGGCC GACATGGAAA CTTGGCAGAG GCTTCGAATG 
TCTTCTGAAG ATAGTGAGCA CTTGAATGAC AGGCAGGCTG ACCAGCAAGA GCAAACATCC 
AAGATTGATG TGACTAGAGC GACAAATGAT GACTTTGGGA GGAGGAAATT GTCTCTGCAA 
GAGAAAAGGG CTCGAAACAA ACAGAAGTTT GTACGCTTTG GCCCAGTGAC TGAAATTGAT 
CAGCAGAAGT GGGATAGGCT TTGTATAGCC AAGGCTGAAT CTGGCCAGGA AAGTGAAGAG 
ATGAAGGGCA GCATTACGTC TGATGAAACT GCCAGCCACA TATCTCCCTC AGCTTCTGCT 
CAGCGCTCCC AACCCCAGGA GGATGTTGGC ACAGGGCCAG TCTTGCCACA TGAGTCAAGA 
TCTTTTACAC CAGAGAGATT GGTTTCACCT GCTCCTCCTG AAAATGAAAG TGAAATTCAG 
CTCCTAGAAA CACAGCAAGA TGACATGATG GCAAGGAGAA CAGGTACATT CCAAAAATGC 
GCCAGAACCA GCAGAGACTC TCCTGTGTCC CACCAATATG CACTCCATAG CCAACAACAC 
GAAGAGAAGT CTGACTCGCT GGAACTCGAA GAGAAACAAG GACTCTTTCT GGATGTTAAC 
CCACAACTGA GAAAATTACT GCTGTCGGAC TTGGCAAATA CTAGTTCAAA AGCAACTAGC 
CCTACTTTGG GGAGCAAGTC TCTGAAAGTC ACCAAAGAAG CTGAAGATGT AAAAAATGAC 
ATGAAAGAAG GTGCCTTGAA CACAGGAAAG GATCCAAACT CTGTTATCCC AGAGGATAAC 
ACAATTGCAC CAGACCCAGA AAAAGATGAC ATGATGACAA GAAAAATCTC AGTGAGCCAG 
AAGCCATCTT GCGTCAGCCT TGGACACTTC CTTCCTGTTC CTTTTTCTTT GCAGCGCAGA 
GAGGGGGAAA AGAAACCAAC AAGCTGCATC ATATCAAGCA CCAAAAAGTC CAAAAATGCA 
TCCAAAGCTG TTCCAGCCGA CATTTCTGAT GTTCCTTCTA AGCCCCTTGA CCAGAAAGAA 
ACAAAAGAAC ATGGTGAATT AGGAGATACG ACTTGCAAGC AAGCCTCCAA TAGTGTGGAG 
ACTCCCATGT GTACAGAGTT AGACAGCCAG CAACAGGATG GTCTTGAGAG GAGAATGATA 
CTGTCTACTG ACTTGGCCAA TACTGAGTTA AAAATAACTC CTCCTACTTT GGCCAACAAC 
TCTCCAACCA TAACAAAAGT ACAAGAGCCA GATGCAACCA TCTCAGAGGA CAATACAACT 
GCACCAAACC CTGAAAAAGA TGATATGATG ACCAGAAGAA TCTCTGTAAC CCAGAAACCA 
TCGTGTGTAG GTCTTGGCCA CTTCCTTCCA GTTCCATTTT CTTTGCAGCA AAAAGTTGAG 
GAAAAGAAAT CAACGAGCTC CACTCTATCG ATCAAAATAA AGTCAGATAA TTGCACCCAA 
GCACTTGCAG TCGGCAGTTC TGACCTTCCT TGTAAGCTCC TCGGCCAATG TGAAACAGAG 
GGACATGTTG AATTACGATC CGATGTGACT TGCGATCAAA CCTCCACTGA GATCATTAGT 
GATGGGATTC CCACTGGTAA AGGGTTAGAT ACCCAGGCAG AAGACGCTCT TGAGACATGT 
GACCATGAGT TATTCAGATT CAGCCCAAGA TCTGTTCTGT CTGATGATGG AGAGAGCATG 
AGCATGTGCG ACATGAGGTA CGAAGAAGAG GGAAGTATGC AGCCTCACAG CAGGGCACAC 
TATGAGCATC TACAGCATGT CAACAGCTTG CTCAAGGAGG AGGATGACAC ATGGCAAGAT 
GACCTAGCTA AGTGGAAGAC ACGGAGAAGA AGTGCATCAC AGGATCTGAT CAAAAAAGAG 
GAACAGAGGA AGAAGATGGA GAGAATGATG AGCGGAGAGG ATGAACTGTC GGAGCGTAGG 
AAAAGCATAA AAACATATAG AGAGATTGTG GAAGAAAAAG AACGTCGAGA GAAAGAACTG 
CATGAAGCCT ACAAAAATGC CAAGTCAAGA GATGAGGCTG AACACATATT ACAGAGCTAC 
ATTGAAAAGT TTACAATTAG TGAAGCTGTT CTTGAGGGAT TAGTAATGCC AAAAATCTTA 
GAAAGAAGCC AGTCGGTGGA GCCAAGTTTA TTGTCTTCAT CAGAGGACCC AAACATCCTG 
AAATACCTGA GGCAACAGTC TTTACCATCA CCAAAATACA CTTCCACTGT TGAAGCAACT 
GTCTCCCCCA GCAGCCCATG TGAAGCAAAA AGTCCAACAG GTCTGATATC ACCAAGCAAA 
AACATTATCT CAAAGACTGT GCCTATGCTA ACAGCCAAGC CTTACTCCCA GCCCAAAAAC 
ACCCAGGAAG TCCTCAAGAC ATTTAAGGTA GATGGAAAGG TAAACCTCAA CGGGGTAGAA 
GAGCAGAAAG AAGAGGAACA TGGAAACCAG ACATTTGCCC CATCACTCAC CCGATCTCAG 
ATGTTTGAAG GTGTAGCCAG AGTTGATGTT CCTGTTGTTG AAATAAAGCA AGACGTAACT 
TCCATAGAGC TTAATCTGGC CAGACCAAAC ACCCTGAATT CCTTACAAGA ACCCAAAAGT 
CTCTGCGAAG ACAGAAATCA CATAAGCCAG GAGGATGAGT CAGATTCTCT GGTGCAATTA 
GAAAGAGCTC ACTCCGTGGA AATATTGTCA GAAGGTAACA CCCAATCAAG CAATGCAACC 
CAGGATGTAC CTGTTGTGAG CAGTATAGAG ATTCCACCAT TGACCATCAA TGGAAAGGAC 
ACTGTGGATG AGAGGAAAGT GGAAGCTGCC GAGTTGATCA TGCATTGGTC CCTGGAGCCG 
AATCATAAGG AAGCCAAAGA AAATTCTTTA TCGCCTTTAA AAACTTCTGA AAATTTAGAA 
GAATCCTTGC AGTTTAAAGA GAATGTGGAT ACAGAATGTA CAGAAAATTC ACCAGCACTT 
GTACCCAGTT TGAATTCTCC TAACCGATTC TGCACATGGG ACCCTGAAGA AGAACGCAAA 
CGGCAAGAAA AATGGCAGCA GGAGCAGGAA AGGCTTCTCC AGGAGAGGTA CCAGAGAGAG 
CAGGAAAAAC TTAAAGAAGA ATGGGAAAAA GCTCAGAAAG AAGTAGAAGA GGAGGAAAGG 
AAATATTATG AGGAGGAAAG AAAGATAATT GAAGACACTG TGGTCCCACT GACTTTGTCT 
CCAAATTCTA ATGCACAGTT TAATATCTCT GAAAGAAATG GGAACTTCAT TCCAGCACTT 
ACAGAACATG TCAAGAGAGA TCAAGAAAAT AACAATGAAA AGCTCTTGAC AGAGTCCAGC 
AGAACGATAA AGAATGAGAC ACATATGCAC AGCAATGTAA CACAGCAACT GCTTTTGCAT 
ACACAGGAGG GGTCAATTCT TGGTGCTAAA GAGAAACAAA TAAAGGGAAA AACAGACAAG 
TGGGAAGCAG AACAGTCCAT CTGTATTATG GACCGAGAGG AGCTGAGCAA CCATGTGCAG 
TCTAATGAAT GTAATACACC CACTAGGTTA CCCTTGCAGG CAGGATGTAA ACAAGTGACG 
TCATCAGAGT CAAGTCTGCC TGGGGATCTT GGATCACCAA AAAATCCTAT CACTGAAAAA 
GATGCTATTC AGAATATGTC GTCCATAATA CCCACACGGG GAACTGATTT CAAGGAAGAT 
ACAATGAATT CTTCACAGTC CTCTACATCA CAGTGCCAGT CACCAAACAG GTCTGTTAGT 
GGGAAAAAAC TGTGCTCCAC ATGTGGACTT CCTCTAGGCA AGGGAGCTGC CATGATAATT 
GAAACCCTCA GTCTTTATTT CCACATCCAG TGCTTCAAGT GTGGACTGTG TAAGGGACAA 
CTTGGGGATG CGATCACCGG CACAGACGTC AGAATTCGAA ATGGCCTACT GAACTGCAAT 
GACTGTTACA TGAAGTCTAG AAGTAAGGCG GCTGGCCACC CCACCCCTTT GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

limch1 ( XM_012955698.3 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012811152.2 Xenopus tropicalis LIM and calponin homology domains 1 (limch1), transcript variant X4, mRNA.

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CloneID	OXa49698
Clone ID Related Accession (Same CDS sequence)	XM_012955698.3
Accession Version	XM_012955698.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5682bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1576512000000
Organism	Xenopus tropicalis(tropical clawed frog)
Product	LIM and calponin homology domains-containing protein 1 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012955698.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCAGCT CCGCCACACT GCAAGGGGAA GAGATCCCCC CGCCCCAGGA GTCTCCCCCA 
GACACGGCTT GCAGGGAGGC ACAGAAGTGG ATTGAGCAAG TTACTGGCAA AAGCTTTGGC 
GACAAAGACT TCAGGACTGG ACTGGAGAAT GGAATCCTTC TGTGCGAGTT ATTAAATGCT 
ATTAAACCAG GACTCGTAAA AAAGATCAAC AGACTTCCTA CTCCCATTGC TGGATTGGAC 
AATATAACAT TATTCCTTAG AGGCTGCAAA GAGCTCGGGC TGAAAGAATC TCAGTTGTTT 
GACCCAGGTG ACCTACAGGA CACAGCAAAC AGGACAACAG CCAGGAATTC AGATTGCAAC 
AGAAAACTAA GAAATGTACT AATCACCGTG TACTGGCTGG GGAAAGCTGC AAGCAGCTGC 
TCGTCCTATA GTGGCAACAA CCTGGACTTA AAGGAGTTTG AGGGATTGCT GGCACAAATG 
AGAAAAGAAA ATGAGGAAAT AGAGAGTCCA AAACGCAGCA TACGGGACAG TGGCTACATA 
GATTGTTGGG ACTCTGAGAG AAGTGACTCC CTTTCTCCTC CCAGACATGG TAGAGATGAC 
TCCTTCGATA GTTTAGACTC ATTTGGATCT AGATCACAAC AGACACCATC TCCAGATGTA 
GTGCTGAGGG GAAGCAGCGA CGGCAGAGGA AGCGACTCTG AGTCTGACCT ACCTCACAGA 
AGGTTACCTG ATGTGCGAAA AGATGATATG TCTGCTAGAC GGGTATCCTA CGGTGAAAAC 
AAAGCATTTG TGCCTTTCAA CCAATACCTC CCCAACAAGA GCAACCAGAC CTCCTACATC 
CCGGCACCCC TGAGGAAGAA GAAGGCTGAG CGTGAGGACT ACCGGAAAAG CTGGAGCACA 
GCTACATCCC CCCTGGGGGG AGAGCGACCC TTCAGTCCCA AACAAAGTCC AGTCATTAAA 
GAAGAACAAA ACGAGTTGCA GTGTGAAGGA GAAGGGACCA AGCAGATGAT TGCCTTAAAA 
GAACCTTGCC TTGGAGCAGA AAATAGGAGT GAGAGAGTTG GTGAAAGTGA AGGGGGTGTC 
ATGGGACAAT CATCTGCTAA TGATGACATT CCAAAAGATG CAGAGGAAGT GAGAAAGCGC 
AAGCACCTTC AGAAAGCGGG TATCAAGGTC TTGCCCGCAA CAAAGCGCTA CAGCAGTCCA 
AGTCAATTTG CTGAAGACAT TGATGGCGCT CCAGACATTG TGCTGCGAAA AGAGAACACC 
TTCCTGAAGT CATATGAAGG GATGGGCTCT GATTCCGAAG AAGAAGAAGA GTGCAAAGTA 
CCTGATATAC AGCGAGATGA CTTGGCCACA AGGCGTGCAA GGATGAACCA ACCAAAAAGC 
CCAATGCAGT TTAATCAGTA TATACAGGGG TCATACTTAA ACAAAAACAG AGCATCAAGA 
AAGCTGAACA GTTCTAAGAC TTCCACTCCA GCTTCTCCAA AAATGTCTCA AGATATCAGG 
GAGTACCAAG GCCCCCAGTT ATTAAAGCCA TCACACGTCT GCAAAGAAAA GACATTTCCA 
ATACCCTCAG ACAATAAAGA GGATGCAGAA GCAGAGCAAG AAAAAGTTCC CGACATTCAG 
AAAGACGACC TGGCGAAACG CAAAGCTAAA GCTTCAGTTT CACTGCCAAA GGAACCATCG 
GCCTTTGCAG CGGTTTCAAT TACAAAGGCC GACATGGAAA CTTGGCAGAG GCTTCGAATG 
TCTTCTGAAG ATAGTGAGCA CTTGAATGAC AGGCAGGCTG ACCAGCAAGA GCAAACATCC 
AAGATTGATG TGACTAGAGC GACAAATGAT GACTTTGGGA GGAGGAAATT GTCTCTGCAA 
GAGAAAAGGG CTCGAAACAA ACAGAAGTTT GTACGCTTTG GCCCAGTGAC TGAAATTGAT 
CAGCAGAAGT GGGATAGGCT TTGTATAGCC AAGGCTGAAT CTGGCCAGGA AAGTGAAGAG 
ATGAAGGGCA GCATTACGTC TGATGAAACT GCCAGCCACA TATCTCCCTC AGCTTCTGCT 
CAGCGCTCCC AACCCCAGGA GGATGTTGGC ACAGGGCCAG TCTTGCCACA TGAGTCAAGA 
TCTTTTACAC CAGAGAGATT GGTTTCACCT GCTCCTCCTG AAAATGAAAG TGAAATTCAG 
CTCCTAGAAA CACAGCAAGA TGACATGATG GCAAGGAGAA CAGGTACATT CCAAAAATGC 
GCCAGAACCA GCAGAGACTC TCCTGTGTCC CACCAATATG CACTCCATAG CCAACAACAC 
GAAGAGAAGT CTGACTCGCT GGAACTCGAA GAGAAACAAG GACTCTTTCT GGATGTTAAC 
CCACAACTGA GAAAATTACT GCTGTCGGAC TTGGCAAATA CTAGTTCAAA AGCAACTAGC 
CCTACTTTGG GGAGCAAGTC TCTGAAAGTC ACCAAAGAAG CTGAAGATGT AAAAAATGAC 
ATGAAAGAAG GTGCCTTGAA CACAGGAAAG GATCCAAACT CTGTTATCCC AGAGGATAAC 
ACAATTGCAC CAGACCCAGA AAAAGATGAC ATGATGACAA GAAAAATCTC AGTGAGCCAG 
AAGCCATCTT GCGTCAGCCT TGGACACTTC CTTCCTGTTC CTTTTTCTTT GCAGCGCAGA 
GAGGGGGAAA AGAAACCAAC AAGCTGCATC ATATCAAGCA CCAAAAAGTC CAAAAATGCA 
TCCAAAGCTG TTCCAGCCGA CATTTCTGAT GTTCCTTCTA AGCCCCTTGA CCAGAAAGAA 
ACAAAAGAAC ATGGTGAATT AGGAGATACG ACTTGCAAGC AAGCCTCCAA TAGTGTGGAG 
ACTCCCATGT GTACAGAGTT AGACAGCCAG CAACAGGATG GTCTTGAGAG GAGAATGATA 
CTGTCTACTG ACTTGGCCAA TACTGAGTTA AAAATAACTC CTCCTACTTT GGCCAACAAC 
TCTCCAACCA TAACAAAAGT ACAAGAGCCA GATGCAACCA TCTCAGAGGA CAATACAACT 
GCACCAAACC CTGAAAAAGA TGATATGATG ACCAGAAGAA TCTCTGTAAC CCAGAAACCA 
TCGTGTGTAG GTCTTGGCCA CTTCCTTCCA GTTCCATTTT CTTTGCAGCA AAAAGTTGAG 
GAAAAGAAAT CAACGAGCTC CACTCTATCG ATCAAAATAA AGTCAGATAA TTGCACCCAA 
GCACTTGCAG TCGGCAGTTC TGACCTTCCT TGTAAGCTCC TCGGCCAATG TGAAACAGAG 
GGACATGTTG AATTACGATC CGATGTGACT TGCGATCAAA CCTCCACTGA GATCATTAGT 
GATGGGATTC CCACTGGTAA AGGGTTAGAT ACCCAGGCAG AAGACGCTCT TGAGACATGT 
GACCATGAGT TATTCAGATT CAGCCCAAGA TCTGTTCTGT CTGATGATGG AGAGAGCATG 
AGCATGTGCG ACATGAGGTA CGAAGAAGAG GGAAGTATGC AGCCTCACAG CAGGGCACAC 
TATGAGCATC TACAGCATGT CAACAGCTTG CTCAAGGAGG AGGATGACAC ATGGCAAGAT 
GACCTAGCTA AGTGGAAGAC ACGGAGAAGA AGTGCATCAC AGGATCTGAT CAAAAAAGAG 
GAACAGAGGA AGAAGATGGA GAGAATGATG AGCGGAGAGG ATGAACTGTC GGAGCGTAGG 
AAAAGCATAA AAACATATAG AGAGATTGTG GAAGAAAAAG AACGTCGAGA GAAAGAACTG 
CATGAAGCCT ACAAAAATGC CAAGTCAAGA GATGAGGCTG AACACATATT ACAGAGCTAC 
ATTGAAAAGT TTACAATTAG TGAAGCTGTT CTTGAGGGAT TAGTAATGCC AAAAATCTTA 
GAAAGAAGCC AGTCGGTGGA GCCAAGTTTA TTGTCTTCAT CAGAGGACCC AAACATCCTG 
AAATACCTGA GGCAACAGTC TTTACCATCA CCAAAATACA CTTCCACTGT TGAAGCAACT 
GTCTCCCCCA GCAGCCCATG TGAAGCAAAA AGTCCAACAG GTCTGATATC ACCAAGCAAA 
AACATTATCT CAAAGACTGT GCCTATGCTA ACAGCCAAGC CTTACTCCCA GCCCAAAAAC 
ACCCAGGAAG TCCTCAAGAC ATTTAAGGTA GATGGAAAGG TAAACCTCAA CGGGGTAGAA 
GAGCAGAAAG AAGAGGAACA TGGAAACCAG ACATTTGCCC CATCACTCAC CCGATCTCAG 
ATGTTTGAAG GTGTAGCCAG AGTTGATGTT CCTGTTGTTG AAATAAAGCA AGACGTAACT 
TCCATAGAGC TTAATCTGGC CAGACCAAAC ACCCTGAATT CCTTACAAGA ACCCAAAAGT 
CTCTGCGAAG ACAGAAATCA CATAAGCCAG GAGGATGAGT CAGATTCTCT GGTGCAATTA 
GAAAGAGCTC ACTCCGTGGA AATATTGTCA GAAGGTAACA CCCAATCAAG CAATGCAACC 
CAGGATGTAC CTGTTGTGAG CAGTATAGAG ATTCCACCAT TGACCATCAA TGGAAAGGAC 
ACTGTGGATG AGAGGAAAGT GGAAGCTGCC GAGTTGATCA TGCATTGGTC CCTGGAGCCG 
AATCATAAGG AAGCCAAAGA AAATTCTTTA TCGCCTTTAA AAACTTCTGA AAATTTAGAA 
GAATCCTTGC AGTTTAAAGA GAATGAATCA TCACACAAAG TGCAGCTAAA CATTACGCTT 
CAGAGCCGGA ACGCCTGGAG ACGCTCCCAG TTTTTTTTGC AGCCAGTGGA TACAGAATGT 
ACAGAAAATT CACCAGCACT TGTACCCAGT TTGAATTCTC CTAACCGATT CTGCACATGG 
GACCCTGAAG AAGAACGCAA ACGGCAAGAA AAATGGCAGC AGGAGCAGGA AAGGCTTCTC 
CAGGAAAGAA AGATAATTGA AGACACTGTG GTCCCACTGA CTTTGTCTCC AAATTCTAAT 
GCACAGTTTA ATATCTCTGA AAGAAATGGG AACTTCATTC CAGCACTTAC AGAACATGTC 
AAGAGAGATC AAGAAAATAA CAATGAAAAG CTCTTGACAG AGTCCAGCAG AACGATAAAG 
AATGAGACAC ATATGCACAG CAATGTAACA CAGCAACTGC TTTTGCATAC ACAGGAGGGG 
TCAATTCTTG GTGCTAAAGA GAAACAAATA AAGGGAAAAA CAGACAAGTG GGAAGCAGAA 
CAGTCCATCT GTATTATGGA CCGAGAGGAG CTGAGCAACC ATGTGCAGTC TAATGAATGT 
AATACACCCA CTAGGTTACC CTTGCAGGCA GGATGTAAAC AAGTGACGTC ATCAGAGTCA 
AGTCTGCCTG GGGATCTTGG ATCACCAAAA AATCCTATCA CTGAAAAAGA TGCTATTCAG 
AATATGTCGT CCATAATACC CACACGGGGA ACTGATTTCA AGGAAGATAC AATGAATTCT 
TCACAGTCCT CTACATCACA GTGCCAGTCA CCAAACAGGT CTGTTAGTGG GAAAAAACTG 
TGCTCCACAT GTGGACTTCC TCTAGGCAAG GGAGCTGCCA TGATAATTGA AACCCTCAGT 
CTTTATTTCC ACATCCAGTG CTTCAAGTGT GGACTGTGTA AGGGACAACT TGGGGATGCG 
ATCACCGGCA CAGACGTCAG AATTCGAAAT GGCCTACTGA ACTGCAATGA CTGTTACATG 
AAGTCTAGAA GTAAGGCGGC TGGCCACCCC ACCCCTTTGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012811152.2
CDS	491..6172
Translation	MASSATLQGEEIPPPQESPPDTACREAQKWIEQVTGKSFGDKDFRTGLENGILLCELLNAIKPGLVKKINRLPTPIAGLDNITLFLRGCKELGLKESQLFDPGDLQDTANRTTARNSDCNRKLRNVLITVYWLGKAASSCSSYSGNNLDLKEFEGLLAQMRKENEEIESPKRSIRDSGYIDCWDSERSDSLSPPRHGRDDSFDSLDSFGSRSQQTPSPDVVLRGSSDGRGSDSESDLPHRRLPDVRKDDMSARRVSYGENKAFVPFNQYLPNKSNQTSYIPAPLRKKKAEREDYRKSWSTATSPLGGERPFSPKQSPVIKEEQNELQCEGEGTKQMIALKEPCLGAENRSERVGESEGGVMGQSSANDDIPKDAEEVRKRKHLQKAGIKVLPATKRYSSPSQFAEDIDGAPDIVLRKENTFLKSYEGMGSDSEEEEECKVPDIQRDDLATRRARMNQPKSPMQFNQYIQGSYLNKNRASRKLNSSKTSTPASPKMSQDIREYQGPQLLKPSHVCKEKTFPIPSDNKEDAEAEQEKVPDIQKDDLAKRKAKASVSLPKEPSAFAAVSITKADMETWQRLRMSSEDSEHLNDRQADQQEQTSKIDVTRATNDDFGRRKLSLQEKRARNKQKFVRFGPVTEIDQQKWDRLCIAKAESGQESEEMKGSITSDETASHISPSASAQRSQPQEDVGTGPVLPHESRSFTPERLVSPAPPENESEIQLLETQQDDMMARRTGTFQKCARTSRDSPVSHQYALHSQQHEEKSDSLELEEKQGLFLDVNPQLRKLLLSDLANTSSKATSPTLGSKSLKVTKEAEDVKNDMKEGALNTGKDPNSVIPEDNTIAPDPEKDDMMTRKISVSQKPSCVSLGHFLPVPFSLQRREGEKKPTSCIISSTKKSKNASKAVPADISDVPSKPLDQKETKEHGELGDTTCKQASNSVETPMCTELDSQQQDGLERRMILSTDLANTELKITPPTLANNSPTITKVQEPDATISEDNTTAPNPEKDDMMTRRISVTQKPSCVGLGHFLPVPFSLQQKVEEKKSTSSTLSIKIKSDNCTQALAVGSSDLPCKLLGQCETEGHVELRSDVTCDQTSTEIISDGIPTGKGLDTQAEDALETCDHELFRFSPRSVLSDDGESMSMCDMRYEEEGSMQPHSRAHYEHLQHVNSLLKEEDDTWQDDLAKWKTRRRSASQDLIKKEEQRKKMERMMSGEDELSERRKSIKTYREIVEEKERREKELHEAYKNAKSRDEAEHILQSYIEKFTISEAVLEGLVMPKILERSQSVEPSLLSSSEDPNILKYLRQQSLPSPKYTSTVEATVSPSSPCEAKSPTGLISPSKNIISKTVPMLTAKPYSQPKNTQEVLKTFKVDGKVNLNGVEEQKEEEHGNQTFAPSLTRSQMFEGVARVDVPVVEIKQDVTSIELNLARPNTLNSLQEPKSLCEDRNHISQEDESDSLVQLERAHSVEILSEGNTQSSNATQDVPVVSSIEIPPLTINGKDTVDERKVEAAELIMHWSLEPNHKEAKENSLSPLKTSENLEESLQFKENESSHKVQLNITLQSRNAWRRSQFFLQPVDTECTENSPALVPSLNSPNRFCTWDPEEERKRQEKWQQEQERLLQERKIIEDTVVPLTLSPNSNAQFNISERNGNFIPALTEHVKRDQENNNEKLLTESSRTIKNETHMHSNVTQQLLLHTQEGSILGAKEKQIKGKTDKWEAEQSICIMDREELSNHVQSNECNTPTRLPLQAGCKQVTSSESSLPGDLGSPKNPITEKDAIQNMSSIIPTRGTDFKEDTMNSSQSSTSQCQSPNRSVSGKKLCSTCGLPLGKGAAMIIETLSLYFHIQCFKCGLCKGQLGDAITGTDVRIRNGLLNCNDCYMKSRSKAAGHPTPL

Target ORF information:

RefSeq Version	XM_012955698.3
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis LIM and calponin homology domains 1 (limch1), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012955698.3

ORF Insert Sequence:

ATGGCCAGCT CCGCCACACT GCAAGGGGAA GAGATCCCCC CGCCCCAGGA GTCTCCCCCA 
GACACGGCTT GCAGGGAGGC ACAGAAGTGG ATTGAGCAAG TTACTGGCAA AAGCTTTGGC 
GACAAAGACT TCAGGACTGG ACTGGAGAAT GGAATCCTTC TGTGCGAGTT ATTAAATGCT 
ATTAAACCAG GACTCGTAAA AAAGATCAAC AGACTTCCTA CTCCCATTGC TGGATTGGAC 
AATATAACAT TATTCCTTAG AGGCTGCAAA GAGCTCGGGC TGAAAGAATC TCAGTTGTTT 
GACCCAGGTG ACCTACAGGA CACAGCAAAC AGGACAACAG CCAGGAATTC AGATTGCAAC 
AGAAAACTAA GAAATGTACT AATCACCGTG TACTGGCTGG GGAAAGCTGC AAGCAGCTGC 
TCGTCCTATA GTGGCAACAA CCTGGACTTA AAGGAGTTTG AGGGATTGCT GGCACAAATG 
AGAAAAGAAA ATGAGGAAAT AGAGAGTCCA AAACGCAGCA TACGGGACAG TGGCTACATA 
GATTGTTGGG ACTCTGAGAG AAGTGACTCC CTTTCTCCTC CCAGACATGG TAGAGATGAC 
TCCTTCGATA GTTTAGACTC ATTTGGATCT AGATCACAAC AGACACCATC TCCAGATGTA 
GTGCTGAGGG GAAGCAGCGA CGGCAGAGGA AGCGACTCTG AGTCTGACCT ACCTCACAGA 
AGGTTACCTG ATGTGCGAAA AGATGATATG TCTGCTAGAC GGGTATCCTA CGGTGAAAAC 
AAAGCATTTG TGCCTTTCAA CCAATACCTC CCCAACAAGA GCAACCAGAC CTCCTACATC 
CCGGCACCCC TGAGGAAGAA GAAGGCTGAG CGTGAGGACT ACCGGAAAAG CTGGAGCACA 
GCTACATCCC CCCTGGGGGG AGAGCGACCC TTCAGTCCCA AACAAAGTCC AGTCATTAAA 
GAAGAACAAA ACGAGTTGCA GTGTGAAGGA GAAGGGACCA AGCAGATGAT TGCCTTAAAA 
GAACCTTGCC TTGGAGCAGA AAATAGGAGT GAGAGAGTTG GTGAAAGTGA AGGGGGTGTC 
ATGGGACAAT CATCTGCTAA TGATGACATT CCAAAAGATG CAGAGGAAGT GAGAAAGCGC 
AAGCACCTTC AGAAAGCGGG TATCAAGGTC TTGCCCGCAA CAAAGCGCTA CAGCAGTCCA 
AGTCAATTTG CTGAAGACAT TGATGGCGCT CCAGACATTG TGCTGCGAAA AGAGAACACC 
TTCCTGAAGT CATATGAAGG GATGGGCTCT GATTCCGAAG AAGAAGAAGA GTGCAAAGTA 
CCTGATATAC AGCGAGATGA CTTGGCCACA AGGCGTGCAA GGATGAACCA ACCAAAAAGC 
CCAATGCAGT TTAATCAGTA TATACAGGGG TCATACTTAA ACAAAAACAG AGCATCAAGA 
AAGCTGAACA GTTCTAAGAC TTCCACTCCA GCTTCTCCAA AAATGTCTCA AGATATCAGG 
GAGTACCAAG GCCCCCAGTT ATTAAAGCCA TCACACGTCT GCAAAGAAAA GACATTTCCA 
ATACCCTCAG ACAATAAAGA GGATGCAGAA GCAGAGCAAG AAAAAGTTCC CGACATTCAG 
AAAGACGACC TGGCGAAACG CAAAGCTAAA GCTTCAGTTT CACTGCCAAA GGAACCATCG 
GCCTTTGCAG CGGTTTCAAT TACAAAGGCC GACATGGAAA CTTGGCAGAG GCTTCGAATG 
TCTTCTGAAG ATAGTGAGCA CTTGAATGAC AGGCAGGCTG ACCAGCAAGA GCAAACATCC 
AAGATTGATG TGACTAGAGC GACAAATGAT GACTTTGGGA GGAGGAAATT GTCTCTGCAA 
GAGAAAAGGG CTCGAAACAA ACAGAAGTTT GTACGCTTTG GCCCAGTGAC TGAAATTGAT 
CAGCAGAAGT GGGATAGGCT TTGTATAGCC AAGGCTGAAT CTGGCCAGGA AAGTGAAGAG 
ATGAAGGGCA GCATTACGTC TGATGAAACT GCCAGCCACA TATCTCCCTC AGCTTCTGCT 
CAGCGCTCCC AACCCCAGGA GGATGTTGGC ACAGGGCCAG TCTTGCCACA TGAGTCAAGA 
TCTTTTACAC CAGAGAGATT GGTTTCACCT GCTCCTCCTG AAAATGAAAG TGAAATTCAG 
CTCCTAGAAA CACAGCAAGA TGACATGATG GCAAGGAGAA CAGGTACATT CCAAAAATGC 
GCCAGAACCA GCAGAGACTC TCCTGTGTCC CACCAATATG CACTCCATAG CCAACAACAC 
GAAGAGAAGT CTGACTCGCT GGAACTCGAA GAGAAACAAG GACTCTTTCT GGATGTTAAC 
CCACAACTGA GAAAATTACT GCTGTCGGAC TTGGCAAATA CTAGTTCAAA AGCAACTAGC 
CCTACTTTGG GGAGCAAGTC TCTGAAAGTC ACCAAAGAAG CTGAAGATGT AAAAAATGAC 
ATGAAAGAAG GTGCCTTGAA CACAGGAAAG GATCCAAACT CTGTTATCCC AGAGGATAAC 
ACAATTGCAC CAGACCCAGA AAAAGATGAC ATGATGACAA GAAAAATCTC AGTGAGCCAG 
AAGCCATCTT GCGTCAGCCT TGGACACTTC CTTCCTGTTC CTTTTTCTTT GCAGCGCAGA 
GAGGGGGAAA AGAAACCAAC AAGCTGCATC ATATCAAGCA CCAAAAAGTC CAAAAATGCA 
TCCAAAGCTG TTCCAGCCGA CATTTCTGAT GTTCCTTCTA AGCCCCTTGA CCAGAAAGAA 
ACAAAAGAAC ATGGTGAATT AGGAGATACG ACTTGCAAGC AAGCCTCCAA TAGTGTGGAG 
ACTCCCATGT GTACAGAGTT AGACAGCCAG CAACAGGATG GTCTTGAGAG GAGAATGATA 
CTGTCTACTG ACTTGGCCAA TACTGAGTTA AAAATAACTC CTCCTACTTT GGCCAACAAC 
TCTCCAACCA TAACAAAAGT ACAAGAGCCA GATGCAACCA TCTCAGAGGA CAATACAACT 
GCACCAAACC CTGAAAAAGA TGATATGATG ACCAGAAGAA TCTCTGTAAC CCAGAAACCA 
TCGTGTGTAG GTCTTGGCCA CTTCCTTCCA GTTCCATTTT CTTTGCAGCA AAAAGTTGAG 
GAAAAGAAAT CAACGAGCTC CACTCTATCG ATCAAAATAA AGTCAGATAA TTGCACCCAA 
GCACTTGCAG TCGGCAGTTC TGACCTTCCT TGTAAGCTCC TCGGCCAATG TGAAACAGAG 
GGACATGTTG AATTACGATC CGATGTGACT TGCGATCAAA CCTCCACTGA GATCATTAGT 
GATGGGATTC CCACTGGTAA AGGGTTAGAT ACCCAGGCAG AAGACGCTCT TGAGACATGT 
GACCATGAGT TATTCAGATT CAGCCCAAGA TCTGTTCTGT CTGATGATGG AGAGAGCATG 
AGCATGTGCG ACATGAGGTA CGAAGAAGAG GGAAGTATGC AGCCTCACAG CAGGGCACAC 
TATGAGCATC TACAGCATGT CAACAGCTTG CTCAAGGAGG AGGATGACAC ATGGCAAGAT 
GACCTAGCTA AGTGGAAGAC ACGGAGAAGA AGTGCATCAC AGGATCTGAT CAAAAAAGAG 
GAACAGAGGA AGAAGATGGA GAGAATGATG AGCGGAGAGG ATGAACTGTC GGAGCGTAGG 
AAAAGCATAA AAACATATAG AGAGATTGTG GAAGAAAAAG AACGTCGAGA GAAAGAACTG 
CATGAAGCCT ACAAAAATGC CAAGTCAAGA GATGAGGCTG AACACATATT ACAGAGCTAC 
ATTGAAAAGT TTACAATTAG TGAAGCTGTT CTTGAGGGAT TAGTAATGCC AAAAATCTTA 
GAAAGAAGCC AGTCGGTGGA GCCAAGTTTA TTGTCTTCAT CAGAGGACCC AAACATCCTG 
AAATACCTGA GGCAACAGTC TTTACCATCA CCAAAATACA CTTCCACTGT TGAAGCAACT 
GTCTCCCCCA GCAGCCCATG TGAAGCAAAA AGTCCAACAG GTCTGATATC ACCAAGCAAA 
AACATTATCT CAAAGACTGT GCCTATGCTA ACAGCCAAGC CTTACTCCCA GCCCAAAAAC 
ACCCAGGAAG TCCTCAAGAC ATTTAAGGTA GATGGAAAGG TAAACCTCAA CGGGGTAGAA 
GAGCAGAAAG AAGAGGAACA TGGAAACCAG ACATTTGCCC CATCACTCAC CCGATCTCAG 
ATGTTTGAAG GTGTAGCCAG AGTTGATGTT CCTGTTGTTG AAATAAAGCA AGACGTAACT 
TCCATAGAGC TTAATCTGGC CAGACCAAAC ACCCTGAATT CCTTACAAGA ACCCAAAAGT 
CTCTGCGAAG ACAGAAATCA CATAAGCCAG GAGGATGAGT CAGATTCTCT GGTGCAATTA 
GAAAGAGCTC ACTCCGTGGA AATATTGTCA GAAGGTAACA CCCAATCAAG CAATGCAACC 
CAGGATGTAC CTGTTGTGAG CAGTATAGAG ATTCCACCAT TGACCATCAA TGGAAAGGAC 
ACTGTGGATG AGAGGAAAGT GGAAGCTGCC GAGTTGATCA TGCATTGGTC CCTGGAGCCG 
AATCATAAGG AAGCCAAAGA AAATTCTTTA TCGCCTTTAA AAACTTCTGA AAATTTAGAA 
GAATCCTTGC AGTTTAAAGA GAATGAATCA TCACACAAAG TGCAGCTAAA CATTACGCTT 
CAGAGCCGGA ACGCCTGGAG ACGCTCCCAG TTTTTTTTGC AGCCAGTGGA TACAGAATGT 
ACAGAAAATT CACCAGCACT TGTACCCAGT TTGAATTCTC CTAACCGATT CTGCACATGG 
GACCCTGAAG AAGAACGCAA ACGGCAAGAA AAATGGCAGC AGGAGCAGGA AAGGCTTCTC 
CAGGAAAGAA AGATAATTGA AGACACTGTG GTCCCACTGA CTTTGTCTCC AAATTCTAAT 
GCACAGTTTA ATATCTCTGA AAGAAATGGG AACTTCATTC CAGCACTTAC AGAACATGTC 
AAGAGAGATC AAGAAAATAA CAATGAAAAG CTCTTGACAG AGTCCAGCAG AACGATAAAG 
AATGAGACAC ATATGCACAG CAATGTAACA CAGCAACTGC TTTTGCATAC ACAGGAGGGG 
TCAATTCTTG GTGCTAAAGA GAAACAAATA AAGGGAAAAA CAGACAAGTG GGAAGCAGAA 
CAGTCCATCT GTATTATGGA CCGAGAGGAG CTGAGCAACC ATGTGCAGTC TAATGAATGT 
AATACACCCA CTAGGTTACC CTTGCAGGCA GGATGTAAAC AAGTGACGTC ATCAGAGTCA 
AGTCTGCCTG GGGATCTTGG ATCACCAAAA AATCCTATCA CTGAAAAAGA TGCTATTCAG 
AATATGTCGT CCATAATACC CACACGGGGA ACTGATTTCA AGGAAGATAC AATGAATTCT 
TCACAGTCCT CTACATCACA GTGCCAGTCA CCAAACAGGT CTGTTAGTGG GAAAAAACTG 
TGCTCCACAT GTGGACTTCC TCTAGGCAAG GGAGCTGCCA TGATAATTGA AACCCTCAGT 
CTTTATTTCC ACATCCAGTG CTTCAAGTGT GGACTGTGTA AGGGACAACT TGGGGATGCG 
ATCACCGGCA CAGACGTCAG AATTCGAAAT GGCCTACTGA ACTGCAATGA CTGTTACATG 
AAGTCTAGAA GTAAGGCGGC TGGCCACCCC ACCCCTTTGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

limch1 ( XM_004911220.4 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_004911277.2 Xenopus tropicalis LIM and calponin homology domains 1 (limch1), transcript variant X5, mRNA.

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CloneID	OXa49699
Clone ID Related Accession (Same CDS sequence)	XM_004911220.4 , XM_004911221.4
Accession Version	XM_004911220.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5604bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1576512000000
Organism	Xenopus tropicalis(tropical clawed frog)
Product	LIM and calponin homology domains-containing protein 1 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_004911220.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCAGCT CCGCCACACT GCAAGGGGAA GAGATCCCCC CGCCCCAGGA GTCTCCCCCA 
GACACGGCTT GCAGGGAGGC ACAGAAGTGG ATTGAGCAAG TTACTGGCAA AAGCTTTGGC 
GACAAAGACT TCAGGACTGG ACTGGAGAAT GGAATCCTTC TGTGCGAGTT ATTAAATGCT 
ATTAAACCAG GACTCGTAAA AAAGATCAAC AGACTTCCTA CTCCCATTGC TGGATTGGAC 
AATATAACAT TATTCCTTAG AGGCTGCAAA GAGCTCGGGC TGAAAGAATC TCAGTTGTTT 
GACCCAGGTG ACCTACAGGA CACAGCAAAC AGGACAACAG CCAGGAATTC AGATTGCAAC 
AGAAAACTAA GAAATGTACT AATCACCGTG TACTGGCTGG GGAAAGCTGC AAGCAGCTGC 
TCGTCCTATA GTGGCAACAA CCTGGACTTA AAGGAGTTTG AGGGATTGCT GGCACAAATG 
AGAAAAGAAA ATGAGGAAAT AGAGAGTCCA AAACGCAGCA TACGGGACAG TGGCTACATA 
GATTGTTGGG ACTCTGAGAG AAGTGACTCC CTTTCTCCTC CCAGACATGG TAGAGATGAC 
TCCTTCGATA GTTTAGACTC ATTTGGATCT AGATCACAAC AGACACCATC TCCAGATGTA 
GTGCTGAGGG GAAGCAGCGA CGGCAGAGGA AGCGACTCTG AGTCTGACCT ACCTCACAGA 
AGGTTACCTG ATGTGCGAAA AGATGATATG TCTGCTAGAC GGGTATCCTA CGGTGAAAAC 
AAAGCATTTG TGCCTTTCAA CCAATACCTC CCCAACAAGA GCAACCAGAC CTCCTACATC 
CCGGCACCCC TGAGGAAGAA GAAGGCTGAG CGTGAGGACT ACCGGAAAAG CTGGAGCACA 
GCTACATCCC CCCTGGGGGG AGAGCGACCC TTCAGTCCCA AACAAAGTCC AGTCATTAAA 
GAAGAACAAA ACGAGTTGCA GTGTGAAGGA GAAGGGACCA AGCAGATGAT TGCCTTAAAA 
GAACCTTGCC TTGGAGCAGA AAATAGGAGT GAGAGAGTTG GTGAAAGTGA AGGGGGTGTC 
ATGGGACAAT CATCTGCTAA TGATGACATT CCAAAAGATG CAGAGGAAGT GAGAAAGCGC 
AAGCACCTTC AGAAAGCGGG TATCAAGGTC TTGCCCGCAA CAAAGCGCTA CAGCAGTCCA 
AGTCAATTTG CTGAAGACAT TGATGGCGCT CCAGACATTG TGCTGCGAAA AGAGAACACC 
TTCCTGAAGT CATATGAAGG GATGGGCTCT GATTCCGAAG AAGAAGAAGA GTGCAAAGTA 
CCTGATATAC AGCGAGATGA CTTGGCCACA AGGCGTGCAA GGATGAACCA ACCAAAAAGC 
CCAATGCAGT TTAATCAGTA TATACAGGGG TCATACTTAA ACAAAAACAG AGCATCAAGA 
AAGCTGAACA GTTCTAAGAC TTCCACTCCA GCTTCTCCAA AAATGTCTCA AGATATCAGG 
GAGTACCAAG GCCCCCAGTT ATTAAAGCCA TCACACGTCT GCAAAGAAAA GACATTTCCA 
ATACCCTCAG ACAATAAAGA GGATGCAGAA GCAGAGCAAG AAAAAGTTCC CGACATTCAG 
AAAGACGACC TGGCGAAACG CAAAGCTAAA GCTTCAGTTT CACTGCCAAA GGAACCATCG 
GCCTTTGCAG CGGTTTCAAT TACAAAGGCC GACATGGAAA CTTGGCAGAG GCTTCGAATG 
TCTTCTGAAG ATAGTGAGCA CTTGAATGAC AGGCAGGCTG ACCAGCAAGA GCAAACATCC 
AAGATTGATG TGACTAGAGC GACAAATGAT GACTTTGGGA GGAGGAAATT GTCTCTGCAA 
GAGAAAAGGG CTCGAAACAA ACAGAAGTTT GTACGCTTTG GCCCAGTGAC TGAAATTGAT 
CAGCAGAAGT GGGATAGGCT TTGTATAGCC AAGGCTGAAT CTGGCCAGGA AAGTGAAGAG 
ATGAAGGGCA GCATTACGTC TGATGAAACT GCCAGCCACA TATCTCCCTC AGCTTCTGCT 
CAGCGCTCCC AACCCCAGGA GGATGTTGGC ACAGGGCCAG TCTTGCCACA TGAGTCAAGA 
TCTTTTACAC CAGAGAGATT GGTTTCACCT GCTCCTCCTG AAAATGAAAG TGAAATTCAG 
CTCCTAGAAA CACAGCAAGA TGACATGATG GCAAGGAGAA CAGGTACATT CCAAAAATGC 
GCCAGAACCA GCAGAGACTC TCCTGTGTCC CACCAATATG CACTCCATAG CCAACAACAC 
GAAGAGAAGT CTGACTCGCT GGAACTCGAA GAGAAACAAG GACTCTTTCT GGATGTTAAC 
CCACAACTGA GAAAATTACT GCTGTCGGAC TTGGCAAATA CTAGTTCAAA AGCAACTAGC 
CCTACTTTGG GGAGCAAGTC TCTGAAAGTC ACCAAAGAAG CTGAAGATGT AAAAAATGAC 
ATGAAAGAAG GTGCCTTGAA CACAGGAAAG GATCCAAACT CTGTTATCCC AGAGGATAAC 
ACAATTGCAC CAGACCCAGA AAAAGATGAC ATGATGACAA GAAAAATCTC AGTGAGCCAG 
AAGCCATCTT GCGTCAGCCT TGGACACTTC CTTCCTGTTC CTTTTTCTTT GCAGCGCAGA 
GAGGGGGAAA AGAAACCAAC AAGCTGCATC ATATCAAGCA CCAAAAAGTC CAAAAATGCA 
TCCAAAGCTG TTCCAGCCGA CATTTCTGAT GTTCCTTCTA AGCCCCTTGA CCAGAAAGAA 
ACAAAAGAAC ATGGTGAATT AGGAGATACG ACTTGCAAGC AAGCCTCCAA TAGTGTGGAG 
ACTCCCATGT GTACAGAGTT AGACAGCCAG CAACAGGATG GTCTTGAGAG GAGAATGATA 
CTGTCTACTG ACTTGGCCAA TACTGAGTTA AAAATAACTC CTCCTACTTT GGCCAACAAC 
TCTCCAACCA TAACAAAAGT ACAAGAGCCA GATGCAACCA TCTCAGAGGA CAATACAACT 
GCACCAAACC CTGAAAAAGA TGATATGATG ACCAGAAGAA TCTCTGTAAC CCAGAAACCA 
TCGTGTGTAG GTCTTGGCCA CTTCCTTCCA GTTCCATTTT CTTTGCAGCA AAAAGTTGAG 
GAAAAGAAAT CAACGAGCTC CACTCTATCG ATCAAAATAA AGTCAGATAA TTGCACCCAA 
GCACTTGCAG TCGGCAGTTC TGACCTTCCT TGTAAGCTCC TCGGCCAATG TGAAACAGAG 
GGACATGTTG AATTACGATC CGATGTGACT TGCGATCAAA CCTCCACTGA GATCATTAGT 
GATGGGATTC CCACTGGTAA AGGGTTAGAT ACCCAGGCAG AAGACGCTCT TGAGACATGT 
GACCATGAGT TATTCAGATT CAGCCCAAGA TCTGTTCTGT CTGATGATGG AGAGAGCATG 
AGCATGTGCG ACATGAGGTA CGAAGAAGAG GGAAGTATGC AGCCTCACAG CAGGGCACAC 
TATGAGCATC TACAGCATGT CAACAGCTTG CTCAAGGAGG AGGATGACAC ATGGCAAGAT 
GACCTAGCTA AGTGGAAGAC ACGGAGAAGA AGTGCATCAC AGGATCTGAT CAAAAAAGAG 
GAACAGAGGA AGAAGATGGA GAGAATGATG AGCGGAGAGG ATGAACTGTC GGAGCGTAGG 
AAAAGCATAA AAACATATAG AGAGATTGTG GAAGAAAAAG AACGTCGAGA GAAAGAACTG 
CATGAAGCCT ACAAAAATGC CAAGTCAAGA GATGAGGCTG AACACATATT ACAGAGCTAC 
ATTGAAAAGT TTACAATTAG TGAAGCTGTT CTTGAGGGAT TAGTAATGCC AAAAATCTTA 
GAAAGAAGCC AGTCGGTGGA GCCAAGTTTA TTGTCTTCAT CAGAGGACCC AAACATCCTG 
AAATACCTGA GGCAACAGTC TTTACCATCA CCAAAATACA CTTCCACTGT TGAAGCAACT 
GTCTCCCCCA GCAGCCCATG TGAAGCAAAA AGTCCAACAG GTCTGATATC ACCAAGCAAA 
AACATTATCT CAAAGACTGT GCCTATGCTA ACAGCCAAGC CTTACTCCCA GCCCAAAAAC 
ACCCAGGAAG TCCTCAAGAC ATTTAAGGTA GATGGAAAGG TAAACCTCAA CGGGGTAGAA 
GAGCAGAAAG AAGAGGAACA TGGAAACCAG ACATTTGCCC CATCACTCAC CCGATCTCAG 
ATGTTTGAAG GTGTAGCCAG AGTTGATGTT CCTGTTGTTG AAATAAAGCA AGACGTAACT 
TCCATAGAGC TTAATCTGGC CAGACCAAAC ACCCTGAATT CCTTACAAGA ACCCAAAAGT 
CTCTGCGAAG ACAGAAATCA CATAAGCCAG GAGGATGAGT CAGATTCTCT GGTGCAATTA 
GAAAGAGCTC ACTCCGTGGA AATATTGTCA GAAGGTAACA CCCAATCAAG CAATGCAACC 
CAGGATGTAC CTGTTGTGAG CAGTATAGAG ATTCCACCAT TGACCATCAA TGGAAAGGAC 
ACTGTGGATG AGAGGAAAGT GGAAGCTGCC GAGTTGATCA TGCATTGGTC CCTGGAGCCG 
AATCATAAGG AAGCCAAAGA AAATTCTTTA TCGCCTTTAA AAACTTCTGA AAATTTAGAA 
GAATCCTTGC AGTTTAAAGA GAATGAATCA TCACACAAAG TGCAGCTAAA CATTACGCTT 
CAGAGCCGGA ACGCCTGGAG ACGCTCCCAG TTTTTTTTGC AGCCAGTGGA TACAGAATGT 
ACAGAAAATT CACCAGCACT TGTACCCAGT TTGAATTCTC CTAACCGATT CTGCACATGG 
GACCCTGAAG AAGAACGCAA ACGGCAAGAA AAATGGCAGC AGGAGCAGGA AAGGCTTCTC 
CAGGAGAGGT ACCAGAGAGA GCAGGAAAAA CTTAAAGAAG AATGGGAAAA AGCTCAGAAA 
GAAGTAGAAG AGGAGGAAAG GAAATATTAT GAGGAGGAAA GAAAGATAAT TGAAGACACT 
GTGGTCCCAC TGACTTTGTC TCCAAATTCT AATGCACAGT TTAATATCTC TGAAAGAAAT 
GGGAACTTCA TTCCAGCACT TACAGAACAT GTCAAGAGAG ATCAAGAAAA TAACAATGAA 
AAGCTCTTGA CAGAGTCCAG CAGAACGATA AAGAATGAGA CACATATGCA CAGCAATGTA 
ACACAGCAAC TGCTTTTGCA TACACAGGAG GGGTCAATTC TTGGTGCTAA AGAGAAACAA 
ATAAAGGGAA AAACAGACAA GTGGGAAGCA GAACAGTCCA TCTGTATTAT GGACCGAGAG 
GAGCTGAGCA ACCATGTGCA GTCTAATGAA TGTAATACAC CCACTAGGTT ACCCTTGCAG 
GCAGGATGTA AACAAGTGAC GTCATCAGAG TCAAGTCTGC CTGGGGATCT TGGATCACCA 
AAAAATCCTA TCACTGAAAA AGATGCTATT CAGAATATGT CGTCCATAAT ACCCACACGG 
GGAACTGATT TCAAGGAAGA TACAATGAAT TCTTCACAGT CCTCTACATC ACAGTGCCAG 
TCACCAAACA GTGTGGACTG TGTAAGGGAC AACTTGGGGA TGCGATCACC GGCACAGACG 
TCAGAATTCG AAATGGCCTA CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004911277.2
CDS	491..6094
Translation	MASSATLQGEEIPPPQESPPDTACREAQKWIEQVTGKSFGDKDFRTGLENGILLCELLNAIKPGLVKKINRLPTPIAGLDNITLFLRGCKELGLKESQLFDPGDLQDTANRTTARNSDCNRKLRNVLITVYWLGKAASSCSSYSGNNLDLKEFEGLLAQMRKENEEIESPKRSIRDSGYIDCWDSERSDSLSPPRHGRDDSFDSLDSFGSRSQQTPSPDVVLRGSSDGRGSDSESDLPHRRLPDVRKDDMSARRVSYGENKAFVPFNQYLPNKSNQTSYIPAPLRKKKAEREDYRKSWSTATSPLGGERPFSPKQSPVIKEEQNELQCEGEGTKQMIALKEPCLGAENRSERVGESEGGVMGQSSANDDIPKDAEEVRKRKHLQKAGIKVLPATKRYSSPSQFAEDIDGAPDIVLRKENTFLKSYEGMGSDSEEEEECKVPDIQRDDLATRRARMNQPKSPMQFNQYIQGSYLNKNRASRKLNSSKTSTPASPKMSQDIREYQGPQLLKPSHVCKEKTFPIPSDNKEDAEAEQEKVPDIQKDDLAKRKAKASVSLPKEPSAFAAVSITKADMETWQRLRMSSEDSEHLNDRQADQQEQTSKIDVTRATNDDFGRRKLSLQEKRARNKQKFVRFGPVTEIDQQKWDRLCIAKAESGQESEEMKGSITSDETASHISPSASAQRSQPQEDVGTGPVLPHESRSFTPERLVSPAPPENESEIQLLETQQDDMMARRTGTFQKCARTSRDSPVSHQYALHSQQHEEKSDSLELEEKQGLFLDVNPQLRKLLLSDLANTSSKATSPTLGSKSLKVTKEAEDVKNDMKEGALNTGKDPNSVIPEDNTIAPDPEKDDMMTRKISVSQKPSCVSLGHFLPVPFSLQRREGEKKPTSCIISSTKKSKNASKAVPADISDVPSKPLDQKETKEHGELGDTTCKQASNSVETPMCTELDSQQQDGLERRMILSTDLANTELKITPPTLANNSPTITKVQEPDATISEDNTTAPNPEKDDMMTRRISVTQKPSCVGLGHFLPVPFSLQQKVEEKKSTSSTLSIKIKSDNCTQALAVGSSDLPCKLLGQCETEGHVELRSDVTCDQTSTEIISDGIPTGKGLDTQAEDALETCDHELFRFSPRSVLSDDGESMSMCDMRYEEEGSMQPHSRAHYEHLQHVNSLLKEEDDTWQDDLAKWKTRRRSASQDLIKKEEQRKKMERMMSGEDELSERRKSIKTYREIVEEKERREKELHEAYKNAKSRDEAEHILQSYIEKFTISEAVLEGLVMPKILERSQSVEPSLLSSSEDPNILKYLRQQSLPSPKYTSTVEATVSPSSPCEAKSPTGLISPSKNIISKTVPMLTAKPYSQPKNTQEVLKTFKVDGKVNLNGVEEQKEEEHGNQTFAPSLTRSQMFEGVARVDVPVVEIKQDVTSIELNLARPNTLNSLQEPKSLCEDRNHISQEDESDSLVQLERAHSVEILSEGNTQSSNATQDVPVVSSIEIPPLTINGKDTVDERKVEAAELIMHWSLEPNHKEAKENSLSPLKTSENLEESLQFKENESSHKVQLNITLQSRNAWRRSQFFLQPVDTECTENSPALVPSLNSPNRFCTWDPEEERKRQEKWQQEQERLLQERYQREQEKLKEEWEKAQKEVEEEERKYYEEERKIIEDTVVPLTLSPNSNAQFNISERNGNFIPALTEHVKRDQENNNEKLLTESSRTIKNETHMHSNVTQQLLLHTQEGSILGAKEKQIKGKTDKWEAEQSICIMDREELSNHVQSNECNTPTRLPLQAGCKQVTSSESSLPGDLGSPKNPITEKDAIQNMSSIIPTRGTDFKEDTMNSSQSSTSQCQSPNSVDCVRDNLGMRSPAQTSEFEMAY

Target ORF information:

RefSeq Version	XM_004911220.4
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis LIM and calponin homology domains 1 (limch1), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004911220.4

ORF Insert Sequence:

ATGGCCAGCT CCGCCACACT GCAAGGGGAA GAGATCCCCC CGCCCCAGGA GTCTCCCCCA 
GACACGGCTT GCAGGGAGGC ACAGAAGTGG ATTGAGCAAG TTACTGGCAA AAGCTTTGGC 
GACAAAGACT TCAGGACTGG ACTGGAGAAT GGAATCCTTC TGTGCGAGTT ATTAAATGCT 
ATTAAACCAG GACTCGTAAA AAAGATCAAC AGACTTCCTA CTCCCATTGC TGGATTGGAC 
AATATAACAT TATTCCTTAG AGGCTGCAAA GAGCTCGGGC TGAAAGAATC TCAGTTGTTT 
GACCCAGGTG ACCTACAGGA CACAGCAAAC AGGACAACAG CCAGGAATTC AGATTGCAAC 
AGAAAACTAA GAAATGTACT AATCACCGTG TACTGGCTGG GGAAAGCTGC AAGCAGCTGC 
TCGTCCTATA GTGGCAACAA CCTGGACTTA AAGGAGTTTG AGGGATTGCT GGCACAAATG 
AGAAAAGAAA ATGAGGAAAT AGAGAGTCCA AAACGCAGCA TACGGGACAG TGGCTACATA 
GATTGTTGGG ACTCTGAGAG AAGTGACTCC CTTTCTCCTC CCAGACATGG TAGAGATGAC 
TCCTTCGATA GTTTAGACTC ATTTGGATCT AGATCACAAC AGACACCATC TCCAGATGTA 
GTGCTGAGGG GAAGCAGCGA CGGCAGAGGA AGCGACTCTG AGTCTGACCT ACCTCACAGA 
AGGTTACCTG ATGTGCGAAA AGATGATATG TCTGCTAGAC GGGTATCCTA CGGTGAAAAC 
AAAGCATTTG TGCCTTTCAA CCAATACCTC CCCAACAAGA GCAACCAGAC CTCCTACATC 
CCGGCACCCC TGAGGAAGAA GAAGGCTGAG CGTGAGGACT ACCGGAAAAG CTGGAGCACA 
GCTACATCCC CCCTGGGGGG AGAGCGACCC TTCAGTCCCA AACAAAGTCC AGTCATTAAA 
GAAGAACAAA ACGAGTTGCA GTGTGAAGGA GAAGGGACCA AGCAGATGAT TGCCTTAAAA 
GAACCTTGCC TTGGAGCAGA AAATAGGAGT GAGAGAGTTG GTGAAAGTGA AGGGGGTGTC 
ATGGGACAAT CATCTGCTAA TGATGACATT CCAAAAGATG CAGAGGAAGT GAGAAAGCGC 
AAGCACCTTC AGAAAGCGGG TATCAAGGTC TTGCCCGCAA CAAAGCGCTA CAGCAGTCCA 
AGTCAATTTG CTGAAGACAT TGATGGCGCT CCAGACATTG TGCTGCGAAA AGAGAACACC 
TTCCTGAAGT CATATGAAGG GATGGGCTCT GATTCCGAAG AAGAAGAAGA GTGCAAAGTA 
CCTGATATAC AGCGAGATGA CTTGGCCACA AGGCGTGCAA GGATGAACCA ACCAAAAAGC 
CCAATGCAGT TTAATCAGTA TATACAGGGG TCATACTTAA ACAAAAACAG AGCATCAAGA 
AAGCTGAACA GTTCTAAGAC TTCCACTCCA GCTTCTCCAA AAATGTCTCA AGATATCAGG 
GAGTACCAAG GCCCCCAGTT ATTAAAGCCA TCACACGTCT GCAAAGAAAA GACATTTCCA 
ATACCCTCAG ACAATAAAGA GGATGCAGAA GCAGAGCAAG AAAAAGTTCC CGACATTCAG 
AAAGACGACC TGGCGAAACG CAAAGCTAAA GCTTCAGTTT CACTGCCAAA GGAACCATCG 
GCCTTTGCAG CGGTTTCAAT TACAAAGGCC GACATGGAAA CTTGGCAGAG GCTTCGAATG 
TCTTCTGAAG ATAGTGAGCA CTTGAATGAC AGGCAGGCTG ACCAGCAAGA GCAAACATCC 
AAGATTGATG TGACTAGAGC GACAAATGAT GACTTTGGGA GGAGGAAATT GTCTCTGCAA 
GAGAAAAGGG CTCGAAACAA ACAGAAGTTT GTACGCTTTG GCCCAGTGAC TGAAATTGAT 
CAGCAGAAGT GGGATAGGCT TTGTATAGCC AAGGCTGAAT CTGGCCAGGA AAGTGAAGAG 
ATGAAGGGCA GCATTACGTC TGATGAAACT GCCAGCCACA TATCTCCCTC AGCTTCTGCT 
CAGCGCTCCC AACCCCAGGA GGATGTTGGC ACAGGGCCAG TCTTGCCACA TGAGTCAAGA 
TCTTTTACAC CAGAGAGATT GGTTTCACCT GCTCCTCCTG AAAATGAAAG TGAAATTCAG 
CTCCTAGAAA CACAGCAAGA TGACATGATG GCAAGGAGAA CAGGTACATT CCAAAAATGC 
GCCAGAACCA GCAGAGACTC TCCTGTGTCC CACCAATATG CACTCCATAG CCAACAACAC 
GAAGAGAAGT CTGACTCGCT GGAACTCGAA GAGAAACAAG GACTCTTTCT GGATGTTAAC 
CCACAACTGA GAAAATTACT GCTGTCGGAC TTGGCAAATA CTAGTTCAAA AGCAACTAGC 
CCTACTTTGG GGAGCAAGTC TCTGAAAGTC ACCAAAGAAG CTGAAGATGT AAAAAATGAC 
ATGAAAGAAG GTGCCTTGAA CACAGGAAAG GATCCAAACT CTGTTATCCC AGAGGATAAC 
ACAATTGCAC CAGACCCAGA AAAAGATGAC ATGATGACAA GAAAAATCTC AGTGAGCCAG 
AAGCCATCTT GCGTCAGCCT TGGACACTTC CTTCCTGTTC CTTTTTCTTT GCAGCGCAGA 
GAGGGGGAAA AGAAACCAAC AAGCTGCATC ATATCAAGCA CCAAAAAGTC CAAAAATGCA 
TCCAAAGCTG TTCCAGCCGA CATTTCTGAT GTTCCTTCTA AGCCCCTTGA CCAGAAAGAA 
ACAAAAGAAC ATGGTGAATT AGGAGATACG ACTTGCAAGC AAGCCTCCAA TAGTGTGGAG 
ACTCCCATGT GTACAGAGTT AGACAGCCAG CAACAGGATG GTCTTGAGAG GAGAATGATA 
CTGTCTACTG ACTTGGCCAA TACTGAGTTA AAAATAACTC CTCCTACTTT GGCCAACAAC 
TCTCCAACCA TAACAAAAGT ACAAGAGCCA GATGCAACCA TCTCAGAGGA CAATACAACT 
GCACCAAACC CTGAAAAAGA TGATATGATG ACCAGAAGAA TCTCTGTAAC CCAGAAACCA 
TCGTGTGTAG GTCTTGGCCA CTTCCTTCCA GTTCCATTTT CTTTGCAGCA AAAAGTTGAG 
GAAAAGAAAT CAACGAGCTC CACTCTATCG ATCAAAATAA AGTCAGATAA TTGCACCCAA 
GCACTTGCAG TCGGCAGTTC TGACCTTCCT TGTAAGCTCC TCGGCCAATG TGAAACAGAG 
GGACATGTTG AATTACGATC CGATGTGACT TGCGATCAAA CCTCCACTGA GATCATTAGT 
GATGGGATTC CCACTGGTAA AGGGTTAGAT ACCCAGGCAG AAGACGCTCT TGAGACATGT 
GACCATGAGT TATTCAGATT CAGCCCAAGA TCTGTTCTGT CTGATGATGG AGAGAGCATG 
AGCATGTGCG ACATGAGGTA CGAAGAAGAG GGAAGTATGC AGCCTCACAG CAGGGCACAC 
TATGAGCATC TACAGCATGT CAACAGCTTG CTCAAGGAGG AGGATGACAC ATGGCAAGAT 
GACCTAGCTA AGTGGAAGAC ACGGAGAAGA AGTGCATCAC AGGATCTGAT CAAAAAAGAG 
GAACAGAGGA AGAAGATGGA GAGAATGATG AGCGGAGAGG ATGAACTGTC GGAGCGTAGG 
AAAAGCATAA AAACATATAG AGAGATTGTG GAAGAAAAAG AACGTCGAGA GAAAGAACTG 
CATGAAGCCT ACAAAAATGC CAAGTCAAGA GATGAGGCTG AACACATATT ACAGAGCTAC 
ATTGAAAAGT TTACAATTAG TGAAGCTGTT CTTGAGGGAT TAGTAATGCC AAAAATCTTA 
GAAAGAAGCC AGTCGGTGGA GCCAAGTTTA TTGTCTTCAT CAGAGGACCC AAACATCCTG 
AAATACCTGA GGCAACAGTC TTTACCATCA CCAAAATACA CTTCCACTGT TGAAGCAACT 
GTCTCCCCCA GCAGCCCATG TGAAGCAAAA AGTCCAACAG GTCTGATATC ACCAAGCAAA 
AACATTATCT CAAAGACTGT GCCTATGCTA ACAGCCAAGC CTTACTCCCA GCCCAAAAAC 
ACCCAGGAAG TCCTCAAGAC ATTTAAGGTA GATGGAAAGG TAAACCTCAA CGGGGTAGAA 
GAGCAGAAAG AAGAGGAACA TGGAAACCAG ACATTTGCCC CATCACTCAC CCGATCTCAG 
ATGTTTGAAG GTGTAGCCAG AGTTGATGTT CCTGTTGTTG AAATAAAGCA AGACGTAACT 
TCCATAGAGC TTAATCTGGC CAGACCAAAC ACCCTGAATT CCTTACAAGA ACCCAAAAGT 
CTCTGCGAAG ACAGAAATCA CATAAGCCAG GAGGATGAGT CAGATTCTCT GGTGCAATTA 
GAAAGAGCTC ACTCCGTGGA AATATTGTCA GAAGGTAACA CCCAATCAAG CAATGCAACC 
CAGGATGTAC CTGTTGTGAG CAGTATAGAG ATTCCACCAT TGACCATCAA TGGAAAGGAC 
ACTGTGGATG AGAGGAAAGT GGAAGCTGCC GAGTTGATCA TGCATTGGTC CCTGGAGCCG 
AATCATAAGG AAGCCAAAGA AAATTCTTTA TCGCCTTTAA AAACTTCTGA AAATTTAGAA 
GAATCCTTGC AGTTTAAAGA GAATGAATCA TCACACAAAG TGCAGCTAAA CATTACGCTT 
CAGAGCCGGA ACGCCTGGAG ACGCTCCCAG TTTTTTTTGC AGCCAGTGGA TACAGAATGT 
ACAGAAAATT CACCAGCACT TGTACCCAGT TTGAATTCTC CTAACCGATT CTGCACATGG 
GACCCTGAAG AAGAACGCAA ACGGCAAGAA AAATGGCAGC AGGAGCAGGA AAGGCTTCTC 
CAGGAGAGGT ACCAGAGAGA GCAGGAAAAA CTTAAAGAAG AATGGGAAAA AGCTCAGAAA 
GAAGTAGAAG AGGAGGAAAG GAAATATTAT GAGGAGGAAA GAAAGATAAT TGAAGACACT 
GTGGTCCCAC TGACTTTGTC TCCAAATTCT AATGCACAGT TTAATATCTC TGAAAGAAAT 
GGGAACTTCA TTCCAGCACT TACAGAACAT GTCAAGAGAG ATCAAGAAAA TAACAATGAA 
AAGCTCTTGA CAGAGTCCAG CAGAACGATA AAGAATGAGA CACATATGCA CAGCAATGTA 
ACACAGCAAC TGCTTTTGCA TACACAGGAG GGGTCAATTC TTGGTGCTAA AGAGAAACAA 
ATAAAGGGAA AAACAGACAA GTGGGAAGCA GAACAGTCCA TCTGTATTAT GGACCGAGAG 
GAGCTGAGCA ACCATGTGCA GTCTAATGAA TGTAATACAC CCACTAGGTT ACCCTTGCAG 
GCAGGATGTA AACAAGTGAC GTCATCAGAG TCAAGTCTGC CTGGGGATCT TGGATCACCA 
AAAAATCCTA TCACTGAAAA AGATGCTATT CAGAATATGT CGTCCATAAT ACCCACACGG 
GGAACTGATT TCAAGGAAGA TACAATGAAT TCTTCACAGT CCTCTACATC ACAGTGCCAG 
TCACCAAACA GTGTGGACTG TGTAAGGGAC AACTTGGGGA TGCGATCACC GGCACAGACG 
TCAGAATTCG AAATGGCCTA CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

limch1 ( XM_004911221.4 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_004911278.2 Xenopus tropicalis LIM and calponin homology domains 1 (limch1), transcript variant X6, mRNA.

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CloneID	OXa49699
Clone ID Related Accession (Same CDS sequence)	XM_004911220.4 , XM_004911221.4
Accession Version	XM_004911221.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5604bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1576512000000
Organism	Xenopus tropicalis(tropical clawed frog)
Product	LIM and calponin homology domains-containing protein 1 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_004911221.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCAGCT CCGCCACACT GCAAGGGGAA GAGATCCCCC CGCCCCAGGA GTCTCCCCCA 
GACACGGCTT GCAGGGAGGC ACAGAAGTGG ATTGAGCAAG TTACTGGCAA AAGCTTTGGC 
GACAAAGACT TCAGGACTGG ACTGGAGAAT GGAATCCTTC TGTGCGAGTT ATTAAATGCT 
ATTAAACCAG GACTCGTAAA AAAGATCAAC AGACTTCCTA CTCCCATTGC TGGATTGGAC 
AATATAACAT TATTCCTTAG AGGCTGCAAA GAGCTCGGGC TGAAAGAATC TCAGTTGTTT 
GACCCAGGTG ACCTACAGGA CACAGCAAAC AGGACAACAG CCAGGAATTC AGATTGCAAC 
AGAAAACTAA GAAATGTACT AATCACCGTG TACTGGCTGG GGAAAGCTGC AAGCAGCTGC 
TCGTCCTATA GTGGCAACAA CCTGGACTTA AAGGAGTTTG AGGGATTGCT GGCACAAATG 
AGAAAAGAAA ATGAGGAAAT AGAGAGTCCA AAACGCAGCA TACGGGACAG TGGCTACATA 
GATTGTTGGG ACTCTGAGAG AAGTGACTCC CTTTCTCCTC CCAGACATGG TAGAGATGAC 
TCCTTCGATA GTTTAGACTC ATTTGGATCT AGATCACAAC AGACACCATC TCCAGATGTA 
GTGCTGAGGG GAAGCAGCGA CGGCAGAGGA AGCGACTCTG AGTCTGACCT ACCTCACAGA 
AGGTTACCTG ATGTGCGAAA AGATGATATG TCTGCTAGAC GGGTATCCTA CGGTGAAAAC 
AAAGCATTTG TGCCTTTCAA CCAATACCTC CCCAACAAGA GCAACCAGAC CTCCTACATC 
CCGGCACCCC TGAGGAAGAA GAAGGCTGAG CGTGAGGACT ACCGGAAAAG CTGGAGCACA 
GCTACATCCC CCCTGGGGGG AGAGCGACCC TTCAGTCCCA AACAAAGTCC AGTCATTAAA 
GAAGAACAAA ACGAGTTGCA GTGTGAAGGA GAAGGGACCA AGCAGATGAT TGCCTTAAAA 
GAACCTTGCC TTGGAGCAGA AAATAGGAGT GAGAGAGTTG GTGAAAGTGA AGGGGGTGTC 
ATGGGACAAT CATCTGCTAA TGATGACATT CCAAAAGATG CAGAGGAAGT GAGAAAGCGC 
AAGCACCTTC AGAAAGCGGG TATCAAGGTC TTGCCCGCAA CAAAGCGCTA CAGCAGTCCA 
AGTCAATTTG CTGAAGACAT TGATGGCGCT CCAGACATTG TGCTGCGAAA AGAGAACACC 
TTCCTGAAGT CATATGAAGG GATGGGCTCT GATTCCGAAG AAGAAGAAGA GTGCAAAGTA 
CCTGATATAC AGCGAGATGA CTTGGCCACA AGGCGTGCAA GGATGAACCA ACCAAAAAGC 
CCAATGCAGT TTAATCAGTA TATACAGGGG TCATACTTAA ACAAAAACAG AGCATCAAGA 
AAGCTGAACA GTTCTAAGAC TTCCACTCCA GCTTCTCCAA AAATGTCTCA AGATATCAGG 
GAGTACCAAG GCCCCCAGTT ATTAAAGCCA TCACACGTCT GCAAAGAAAA GACATTTCCA 
ATACCCTCAG ACAATAAAGA GGATGCAGAA GCAGAGCAAG AAAAAGTTCC CGACATTCAG 
AAAGACGACC TGGCGAAACG CAAAGCTAAA GCTTCAGTTT CACTGCCAAA GGAACCATCG 
GCCTTTGCAG CGGTTTCAAT TACAAAGGCC GACATGGAAA CTTGGCAGAG GCTTCGAATG 
TCTTCTGAAG ATAGTGAGCA CTTGAATGAC AGGCAGGCTG ACCAGCAAGA GCAAACATCC 
AAGATTGATG TGACTAGAGC GACAAATGAT GACTTTGGGA GGAGGAAATT GTCTCTGCAA 
GAGAAAAGGG CTCGAAACAA ACAGAAGTTT GTACGCTTTG GCCCAGTGAC TGAAATTGAT 
CAGCAGAAGT GGGATAGGCT TTGTATAGCC AAGGCTGAAT CTGGCCAGGA AAGTGAAGAG 
ATGAAGGGCA GCATTACGTC TGATGAAACT GCCAGCCACA TATCTCCCTC AGCTTCTGCT 
CAGCGCTCCC AACCCCAGGA GGATGTTGGC ACAGGGCCAG TCTTGCCACA TGAGTCAAGA 
TCTTTTACAC CAGAGAGATT GGTTTCACCT GCTCCTCCTG AAAATGAAAG TGAAATTCAG 
CTCCTAGAAA CACAGCAAGA TGACATGATG GCAAGGAGAA CAGGTACATT CCAAAAATGC 
GCCAGAACCA GCAGAGACTC TCCTGTGTCC CACCAATATG CACTCCATAG CCAACAACAC 
GAAGAGAAGT CTGACTCGCT GGAACTCGAA GAGAAACAAG GACTCTTTCT GGATGTTAAC 
CCACAACTGA GAAAATTACT GCTGTCGGAC TTGGCAAATA CTAGTTCAAA AGCAACTAGC 
CCTACTTTGG GGAGCAAGTC TCTGAAAGTC ACCAAAGAAG CTGAAGATGT AAAAAATGAC 
ATGAAAGAAG GTGCCTTGAA CACAGGAAAG GATCCAAACT CTGTTATCCC AGAGGATAAC 
ACAATTGCAC CAGACCCAGA AAAAGATGAC ATGATGACAA GAAAAATCTC AGTGAGCCAG 
AAGCCATCTT GCGTCAGCCT TGGACACTTC CTTCCTGTTC CTTTTTCTTT GCAGCGCAGA 
GAGGGGGAAA AGAAACCAAC AAGCTGCATC ATATCAAGCA CCAAAAAGTC CAAAAATGCA 
TCCAAAGCTG TTCCAGCCGA CATTTCTGAT GTTCCTTCTA AGCCCCTTGA CCAGAAAGAA 
ACAAAAGAAC ATGGTGAATT AGGAGATACG ACTTGCAAGC AAGCCTCCAA TAGTGTGGAG 
ACTCCCATGT GTACAGAGTT AGACAGCCAG CAACAGGATG GTCTTGAGAG GAGAATGATA 
CTGTCTACTG ACTTGGCCAA TACTGAGTTA AAAATAACTC CTCCTACTTT GGCCAACAAC 
TCTCCAACCA TAACAAAAGT ACAAGAGCCA GATGCAACCA TCTCAGAGGA CAATACAACT 
GCACCAAACC CTGAAAAAGA TGATATGATG ACCAGAAGAA TCTCTGTAAC CCAGAAACCA 
TCGTGTGTAG GTCTTGGCCA CTTCCTTCCA GTTCCATTTT CTTTGCAGCA AAAAGTTGAG 
GAAAAGAAAT CAACGAGCTC CACTCTATCG ATCAAAATAA AGTCAGATAA TTGCACCCAA 
GCACTTGCAG TCGGCAGTTC TGACCTTCCT TGTAAGCTCC TCGGCCAATG TGAAACAGAG 
GGACATGTTG AATTACGATC CGATGTGACT TGCGATCAAA CCTCCACTGA GATCATTAGT 
GATGGGATTC CCACTGGTAA AGGGTTAGAT ACCCAGGCAG AAGACGCTCT TGAGACATGT 
GACCATGAGT TATTCAGATT CAGCCCAAGA TCTGTTCTGT CTGATGATGG AGAGAGCATG 
AGCATGTGCG ACATGAGGTA CGAAGAAGAG GGAAGTATGC AGCCTCACAG CAGGGCACAC 
TATGAGCATC TACAGCATGT CAACAGCTTG CTCAAGGAGG AGGATGACAC ATGGCAAGAT 
GACCTAGCTA AGTGGAAGAC ACGGAGAAGA AGTGCATCAC AGGATCTGAT CAAAAAAGAG 
GAACAGAGGA AGAAGATGGA GAGAATGATG AGCGGAGAGG ATGAACTGTC GGAGCGTAGG 
AAAAGCATAA AAACATATAG AGAGATTGTG GAAGAAAAAG AACGTCGAGA GAAAGAACTG 
CATGAAGCCT ACAAAAATGC CAAGTCAAGA GATGAGGCTG AACACATATT ACAGAGCTAC 
ATTGAAAAGT TTACAATTAG TGAAGCTGTT CTTGAGGGAT TAGTAATGCC AAAAATCTTA 
GAAAGAAGCC AGTCGGTGGA GCCAAGTTTA TTGTCTTCAT CAGAGGACCC AAACATCCTG 
AAATACCTGA GGCAACAGTC TTTACCATCA CCAAAATACA CTTCCACTGT TGAAGCAACT 
GTCTCCCCCA GCAGCCCATG TGAAGCAAAA AGTCCAACAG GTCTGATATC ACCAAGCAAA 
AACATTATCT CAAAGACTGT GCCTATGCTA ACAGCCAAGC CTTACTCCCA GCCCAAAAAC 
ACCCAGGAAG TCCTCAAGAC ATTTAAGGTA GATGGAAAGG TAAACCTCAA CGGGGTAGAA 
GAGCAGAAAG AAGAGGAACA TGGAAACCAG ACATTTGCCC CATCACTCAC CCGATCTCAG 
ATGTTTGAAG GTGTAGCCAG AGTTGATGTT CCTGTTGTTG AAATAAAGCA AGACGTAACT 
TCCATAGAGC TTAATCTGGC CAGACCAAAC ACCCTGAATT CCTTACAAGA ACCCAAAAGT 
CTCTGCGAAG ACAGAAATCA CATAAGCCAG GAGGATGAGT CAGATTCTCT GGTGCAATTA 
GAAAGAGCTC ACTCCGTGGA AATATTGTCA GAAGGTAACA CCCAATCAAG CAATGCAACC 
CAGGATGTAC CTGTTGTGAG CAGTATAGAG ATTCCACCAT TGACCATCAA TGGAAAGGAC 
ACTGTGGATG AGAGGAAAGT GGAAGCTGCC GAGTTGATCA TGCATTGGTC CCTGGAGCCG 
AATCATAAGG AAGCCAAAGA AAATTCTTTA TCGCCTTTAA AAACTTCTGA AAATTTAGAA 
GAATCCTTGC AGTTTAAAGA GAATGAATCA TCACACAAAG TGCAGCTAAA CATTACGCTT 
CAGAGCCGGA ACGCCTGGAG ACGCTCCCAG TTTTTTTTGC AGCCAGTGGA TACAGAATGT 
ACAGAAAATT CACCAGCACT TGTACCCAGT TTGAATTCTC CTAACCGATT CTGCACATGG 
GACCCTGAAG AAGAACGCAA ACGGCAAGAA AAATGGCAGC AGGAGCAGGA AAGGCTTCTC 
CAGGAGAGGT ACCAGAGAGA GCAGGAAAAA CTTAAAGAAG AATGGGAAAA AGCTCAGAAA 
GAAGTAGAAG AGGAGGAAAG GAAATATTAT GAGGAGGAAA GAAAGATAAT TGAAGACACT 
GTGGTCCCAC TGACTTTGTC TCCAAATTCT AATGCACAGT TTAATATCTC TGAAAGAAAT 
GGGAACTTCA TTCCAGCACT TACAGAACAT GTCAAGAGAG ATCAAGAAAA TAACAATGAA 
AAGCTCTTGA CAGAGTCCAG CAGAACGATA AAGAATGAGA CACATATGCA CAGCAATGTA 
ACACAGCAAC TGCTTTTGCA TACACAGGAG GGGTCAATTC TTGGTGCTAA AGAGAAACAA 
ATAAAGGGAA AAACAGACAA GTGGGAAGCA GAACAGTCCA TCTGTATTAT GGACCGAGAG 
GAGCTGAGCA ACCATGTGCA GTCTAATGAA TGTAATACAC CCACTAGGTT ACCCTTGCAG 
GCAGGATGTA AACAAGTGAC GTCATCAGAG TCAAGTCTGC CTGGGGATCT TGGATCACCA 
AAAAATCCTA TCACTGAAAA AGATGCTATT CAGAATATGT CGTCCATAAT ACCCACACGG 
GGAACTGATT TCAAGGAAGA TACAATGAAT TCTTCACAGT CCTCTACATC ACAGTGCCAG 
TCACCAAACA GTGTGGACTG TGTAAGGGAC AACTTGGGGA TGCGATCACC GGCACAGACG 
TCAGAATTCG AAATGGCCTA CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004911278.2
CDS	491..6094
Translation	MASSATLQGEEIPPPQESPPDTACREAQKWIEQVTGKSFGDKDFRTGLENGILLCELLNAIKPGLVKKINRLPTPIAGLDNITLFLRGCKELGLKESQLFDPGDLQDTANRTTARNSDCNRKLRNVLITVYWLGKAASSCSSYSGNNLDLKEFEGLLAQMRKENEEIESPKRSIRDSGYIDCWDSERSDSLSPPRHGRDDSFDSLDSFGSRSQQTPSPDVVLRGSSDGRGSDSESDLPHRRLPDVRKDDMSARRVSYGENKAFVPFNQYLPNKSNQTSYIPAPLRKKKAEREDYRKSWSTATSPLGGERPFSPKQSPVIKEEQNELQCEGEGTKQMIALKEPCLGAENRSERVGESEGGVMGQSSANDDIPKDAEEVRKRKHLQKAGIKVLPATKRYSSPSQFAEDIDGAPDIVLRKENTFLKSYEGMGSDSEEEEECKVPDIQRDDLATRRARMNQPKSPMQFNQYIQGSYLNKNRASRKLNSSKTSTPASPKMSQDIREYQGPQLLKPSHVCKEKTFPIPSDNKEDAEAEQEKVPDIQKDDLAKRKAKASVSLPKEPSAFAAVSITKADMETWQRLRMSSEDSEHLNDRQADQQEQTSKIDVTRATNDDFGRRKLSLQEKRARNKQKFVRFGPVTEIDQQKWDRLCIAKAESGQESEEMKGSITSDETASHISPSASAQRSQPQEDVGTGPVLPHESRSFTPERLVSPAPPENESEIQLLETQQDDMMARRTGTFQKCARTSRDSPVSHQYALHSQQHEEKSDSLELEEKQGLFLDVNPQLRKLLLSDLANTSSKATSPTLGSKSLKVTKEAEDVKNDMKEGALNTGKDPNSVIPEDNTIAPDPEKDDMMTRKISVSQKPSCVSLGHFLPVPFSLQRREGEKKPTSCIISSTKKSKNASKAVPADISDVPSKPLDQKETKEHGELGDTTCKQASNSVETPMCTELDSQQQDGLERRMILSTDLANTELKITPPTLANNSPTITKVQEPDATISEDNTTAPNPEKDDMMTRRISVTQKPSCVGLGHFLPVPFSLQQKVEEKKSTSSTLSIKIKSDNCTQALAVGSSDLPCKLLGQCETEGHVELRSDVTCDQTSTEIISDGIPTGKGLDTQAEDALETCDHELFRFSPRSVLSDDGESMSMCDMRYEEEGSMQPHSRAHYEHLQHVNSLLKEEDDTWQDDLAKWKTRRRSASQDLIKKEEQRKKMERMMSGEDELSERRKSIKTYREIVEEKERREKELHEAYKNAKSRDEAEHILQSYIEKFTISEAVLEGLVMPKILERSQSVEPSLLSSSEDPNILKYLRQQSLPSPKYTSTVEATVSPSSPCEAKSPTGLISPSKNIISKTVPMLTAKPYSQPKNTQEVLKTFKVDGKVNLNGVEEQKEEEHGNQTFAPSLTRSQMFEGVARVDVPVVEIKQDVTSIELNLARPNTLNSLQEPKSLCEDRNHISQEDESDSLVQLERAHSVEILSEGNTQSSNATQDVPVVSSIEIPPLTINGKDTVDERKVEAAELIMHWSLEPNHKEAKENSLSPLKTSENLEESLQFKENESSHKVQLNITLQSRNAWRRSQFFLQPVDTECTENSPALVPSLNSPNRFCTWDPEEERKRQEKWQQEQERLLQERYQREQEKLKEEWEKAQKEVEEEERKYYEEERKIIEDTVVPLTLSPNSNAQFNISERNGNFIPALTEHVKRDQENNNEKLLTESSRTIKNETHMHSNVTQQLLLHTQEGSILGAKEKQIKGKTDKWEAEQSICIMDREELSNHVQSNECNTPTRLPLQAGCKQVTSSESSLPGDLGSPKNPITEKDAIQNMSSIIPTRGTDFKEDTMNSSQSSTSQCQSPNSVDCVRDNLGMRSPAQTSEFEMAY

Target ORF information:

RefSeq Version	XM_004911221.4
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis LIM and calponin homology domains 1 (limch1), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004911221.4

ORF Insert Sequence:

ATGGCCAGCT CCGCCACACT GCAAGGGGAA GAGATCCCCC CGCCCCAGGA GTCTCCCCCA 
GACACGGCTT GCAGGGAGGC ACAGAAGTGG ATTGAGCAAG TTACTGGCAA AAGCTTTGGC 
GACAAAGACT TCAGGACTGG ACTGGAGAAT GGAATCCTTC TGTGCGAGTT ATTAAATGCT 
ATTAAACCAG GACTCGTAAA AAAGATCAAC AGACTTCCTA CTCCCATTGC TGGATTGGAC 
AATATAACAT TATTCCTTAG AGGCTGCAAA GAGCTCGGGC TGAAAGAATC TCAGTTGTTT 
GACCCAGGTG ACCTACAGGA CACAGCAAAC AGGACAACAG CCAGGAATTC AGATTGCAAC 
AGAAAACTAA GAAATGTACT AATCACCGTG TACTGGCTGG GGAAAGCTGC AAGCAGCTGC 
TCGTCCTATA GTGGCAACAA CCTGGACTTA AAGGAGTTTG AGGGATTGCT GGCACAAATG 
AGAAAAGAAA ATGAGGAAAT AGAGAGTCCA AAACGCAGCA TACGGGACAG TGGCTACATA 
GATTGTTGGG ACTCTGAGAG AAGTGACTCC CTTTCTCCTC CCAGACATGG TAGAGATGAC 
TCCTTCGATA GTTTAGACTC ATTTGGATCT AGATCACAAC AGACACCATC TCCAGATGTA 
GTGCTGAGGG GAAGCAGCGA CGGCAGAGGA AGCGACTCTG AGTCTGACCT ACCTCACAGA 
AGGTTACCTG ATGTGCGAAA AGATGATATG TCTGCTAGAC GGGTATCCTA CGGTGAAAAC 
AAAGCATTTG TGCCTTTCAA CCAATACCTC CCCAACAAGA GCAACCAGAC CTCCTACATC 
CCGGCACCCC TGAGGAAGAA GAAGGCTGAG CGTGAGGACT ACCGGAAAAG CTGGAGCACA 
GCTACATCCC CCCTGGGGGG AGAGCGACCC TTCAGTCCCA AACAAAGTCC AGTCATTAAA 
GAAGAACAAA ACGAGTTGCA GTGTGAAGGA GAAGGGACCA AGCAGATGAT TGCCTTAAAA 
GAACCTTGCC TTGGAGCAGA AAATAGGAGT GAGAGAGTTG GTGAAAGTGA AGGGGGTGTC 
ATGGGACAAT CATCTGCTAA TGATGACATT CCAAAAGATG CAGAGGAAGT GAGAAAGCGC 
AAGCACCTTC AGAAAGCGGG TATCAAGGTC TTGCCCGCAA CAAAGCGCTA CAGCAGTCCA 
AGTCAATTTG CTGAAGACAT TGATGGCGCT CCAGACATTG TGCTGCGAAA AGAGAACACC 
TTCCTGAAGT CATATGAAGG GATGGGCTCT GATTCCGAAG AAGAAGAAGA GTGCAAAGTA 
CCTGATATAC AGCGAGATGA CTTGGCCACA AGGCGTGCAA GGATGAACCA ACCAAAAAGC 
CCAATGCAGT TTAATCAGTA TATACAGGGG TCATACTTAA ACAAAAACAG AGCATCAAGA 
AAGCTGAACA GTTCTAAGAC TTCCACTCCA GCTTCTCCAA AAATGTCTCA AGATATCAGG 
GAGTACCAAG GCCCCCAGTT ATTAAAGCCA TCACACGTCT GCAAAGAAAA GACATTTCCA 
ATACCCTCAG ACAATAAAGA GGATGCAGAA GCAGAGCAAG AAAAAGTTCC CGACATTCAG 
AAAGACGACC TGGCGAAACG CAAAGCTAAA GCTTCAGTTT CACTGCCAAA GGAACCATCG 
GCCTTTGCAG CGGTTTCAAT TACAAAGGCC GACATGGAAA CTTGGCAGAG GCTTCGAATG 
TCTTCTGAAG ATAGTGAGCA CTTGAATGAC AGGCAGGCTG ACCAGCAAGA GCAAACATCC 
AAGATTGATG TGACTAGAGC GACAAATGAT GACTTTGGGA GGAGGAAATT GTCTCTGCAA 
GAGAAAAGGG CTCGAAACAA ACAGAAGTTT GTACGCTTTG GCCCAGTGAC TGAAATTGAT 
CAGCAGAAGT GGGATAGGCT TTGTATAGCC AAGGCTGAAT CTGGCCAGGA AAGTGAAGAG 
ATGAAGGGCA GCATTACGTC TGATGAAACT GCCAGCCACA TATCTCCCTC AGCTTCTGCT 
CAGCGCTCCC AACCCCAGGA GGATGTTGGC ACAGGGCCAG TCTTGCCACA TGAGTCAAGA 
TCTTTTACAC CAGAGAGATT GGTTTCACCT GCTCCTCCTG AAAATGAAAG TGAAATTCAG 
CTCCTAGAAA CACAGCAAGA TGACATGATG GCAAGGAGAA CAGGTACATT CCAAAAATGC 
GCCAGAACCA GCAGAGACTC TCCTGTGTCC CACCAATATG CACTCCATAG CCAACAACAC 
GAAGAGAAGT CTGACTCGCT GGAACTCGAA GAGAAACAAG GACTCTTTCT GGATGTTAAC 
CCACAACTGA GAAAATTACT GCTGTCGGAC TTGGCAAATA CTAGTTCAAA AGCAACTAGC 
CCTACTTTGG GGAGCAAGTC TCTGAAAGTC ACCAAAGAAG CTGAAGATGT AAAAAATGAC 
ATGAAAGAAG GTGCCTTGAA CACAGGAAAG GATCCAAACT CTGTTATCCC AGAGGATAAC 
ACAATTGCAC CAGACCCAGA AAAAGATGAC ATGATGACAA GAAAAATCTC AGTGAGCCAG 
AAGCCATCTT GCGTCAGCCT TGGACACTTC CTTCCTGTTC CTTTTTCTTT GCAGCGCAGA 
GAGGGGGAAA AGAAACCAAC AAGCTGCATC ATATCAAGCA CCAAAAAGTC CAAAAATGCA 
TCCAAAGCTG TTCCAGCCGA CATTTCTGAT GTTCCTTCTA AGCCCCTTGA CCAGAAAGAA 
ACAAAAGAAC ATGGTGAATT AGGAGATACG ACTTGCAAGC AAGCCTCCAA TAGTGTGGAG 
ACTCCCATGT GTACAGAGTT AGACAGCCAG CAACAGGATG GTCTTGAGAG GAGAATGATA 
CTGTCTACTG ACTTGGCCAA TACTGAGTTA AAAATAACTC CTCCTACTTT GGCCAACAAC 
TCTCCAACCA TAACAAAAGT ACAAGAGCCA GATGCAACCA TCTCAGAGGA CAATACAACT 
GCACCAAACC CTGAAAAAGA TGATATGATG ACCAGAAGAA TCTCTGTAAC CCAGAAACCA 
TCGTGTGTAG GTCTTGGCCA CTTCCTTCCA GTTCCATTTT CTTTGCAGCA AAAAGTTGAG 
GAAAAGAAAT CAACGAGCTC CACTCTATCG ATCAAAATAA AGTCAGATAA TTGCACCCAA 
GCACTTGCAG TCGGCAGTTC TGACCTTCCT TGTAAGCTCC TCGGCCAATG TGAAACAGAG 
GGACATGTTG AATTACGATC CGATGTGACT TGCGATCAAA CCTCCACTGA GATCATTAGT 
GATGGGATTC CCACTGGTAA AGGGTTAGAT ACCCAGGCAG AAGACGCTCT TGAGACATGT 
GACCATGAGT TATTCAGATT CAGCCCAAGA TCTGTTCTGT CTGATGATGG AGAGAGCATG 
AGCATGTGCG ACATGAGGTA CGAAGAAGAG GGAAGTATGC AGCCTCACAG CAGGGCACAC 
TATGAGCATC TACAGCATGT CAACAGCTTG CTCAAGGAGG AGGATGACAC ATGGCAAGAT 
GACCTAGCTA AGTGGAAGAC ACGGAGAAGA AGTGCATCAC AGGATCTGAT CAAAAAAGAG 
GAACAGAGGA AGAAGATGGA GAGAATGATG AGCGGAGAGG ATGAACTGTC GGAGCGTAGG 
AAAAGCATAA AAACATATAG AGAGATTGTG GAAGAAAAAG AACGTCGAGA GAAAGAACTG 
CATGAAGCCT ACAAAAATGC CAAGTCAAGA GATGAGGCTG AACACATATT ACAGAGCTAC 
ATTGAAAAGT TTACAATTAG TGAAGCTGTT CTTGAGGGAT TAGTAATGCC AAAAATCTTA 
GAAAGAAGCC AGTCGGTGGA GCCAAGTTTA TTGTCTTCAT CAGAGGACCC AAACATCCTG 
AAATACCTGA GGCAACAGTC TTTACCATCA CCAAAATACA CTTCCACTGT TGAAGCAACT 
GTCTCCCCCA GCAGCCCATG TGAAGCAAAA AGTCCAACAG GTCTGATATC ACCAAGCAAA 
AACATTATCT CAAAGACTGT GCCTATGCTA ACAGCCAAGC CTTACTCCCA GCCCAAAAAC 
ACCCAGGAAG TCCTCAAGAC ATTTAAGGTA GATGGAAAGG TAAACCTCAA CGGGGTAGAA 
GAGCAGAAAG AAGAGGAACA TGGAAACCAG ACATTTGCCC CATCACTCAC CCGATCTCAG 
ATGTTTGAAG GTGTAGCCAG AGTTGATGTT CCTGTTGTTG AAATAAAGCA AGACGTAACT 
TCCATAGAGC TTAATCTGGC CAGACCAAAC ACCCTGAATT CCTTACAAGA ACCCAAAAGT 
CTCTGCGAAG ACAGAAATCA CATAAGCCAG GAGGATGAGT CAGATTCTCT GGTGCAATTA 
GAAAGAGCTC ACTCCGTGGA AATATTGTCA GAAGGTAACA CCCAATCAAG CAATGCAACC 
CAGGATGTAC CTGTTGTGAG CAGTATAGAG ATTCCACCAT TGACCATCAA TGGAAAGGAC 
ACTGTGGATG AGAGGAAAGT GGAAGCTGCC GAGTTGATCA TGCATTGGTC CCTGGAGCCG 
AATCATAAGG AAGCCAAAGA AAATTCTTTA TCGCCTTTAA AAACTTCTGA AAATTTAGAA 
GAATCCTTGC AGTTTAAAGA GAATGAATCA TCACACAAAG TGCAGCTAAA CATTACGCTT 
CAGAGCCGGA ACGCCTGGAG ACGCTCCCAG TTTTTTTTGC AGCCAGTGGA TACAGAATGT 
ACAGAAAATT CACCAGCACT TGTACCCAGT TTGAATTCTC CTAACCGATT CTGCACATGG 
GACCCTGAAG AAGAACGCAA ACGGCAAGAA AAATGGCAGC AGGAGCAGGA AAGGCTTCTC 
CAGGAGAGGT ACCAGAGAGA GCAGGAAAAA CTTAAAGAAG AATGGGAAAA AGCTCAGAAA 
GAAGTAGAAG AGGAGGAAAG GAAATATTAT GAGGAGGAAA GAAAGATAAT TGAAGACACT 
GTGGTCCCAC TGACTTTGTC TCCAAATTCT AATGCACAGT TTAATATCTC TGAAAGAAAT 
GGGAACTTCA TTCCAGCACT TACAGAACAT GTCAAGAGAG ATCAAGAAAA TAACAATGAA 
AAGCTCTTGA CAGAGTCCAG CAGAACGATA AAGAATGAGA CACATATGCA CAGCAATGTA 
ACACAGCAAC TGCTTTTGCA TACACAGGAG GGGTCAATTC TTGGTGCTAA AGAGAAACAA 
ATAAAGGGAA AAACAGACAA GTGGGAAGCA GAACAGTCCA TCTGTATTAT GGACCGAGAG 
GAGCTGAGCA ACCATGTGCA GTCTAATGAA TGTAATACAC CCACTAGGTT ACCCTTGCAG 
GCAGGATGTA AACAAGTGAC GTCATCAGAG TCAAGTCTGC CTGGGGATCT TGGATCACCA 
AAAAATCCTA TCACTGAAAA AGATGCTATT CAGAATATGT CGTCCATAAT ACCCACACGG 
GGAACTGATT TCAAGGAAGA TACAATGAAT TCTTCACAGT CCTCTACATC ACAGTGCCAG 
TCACCAAACA GTGTGGACTG TGTAAGGGAC AACTTGGGGA TGCGATCACC GGCACAGACG 
TCAGAATTCG AAATGGCCTA CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

limch1 ( XM_031895385.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_031751245.1 Xenopus tropicalis LIM and calponin homology domains 1 (limch1), transcript variant X7, mRNA.

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CloneID	OXa49700
Clone ID Related Accession (Same CDS sequence)	XM_031895385.1
Accession Version	XM_031895385.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5535bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1576512000000
Organism	Xenopus tropicalis(tropical clawed frog)
Product	LIM and calponin homology domains-containing protein 1 isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCAGCT CCGCCACACT GCAAGGGGAA GAGATCCCCC CGCCCCAGGA GTCTCCCCCA 
GACACGGCTT GCAGGGAGGC ACAGAAGTGG ATTGAGCAAG TTACTGGCAA AAGCTTTGGC 
GACAAAGACT TCAGGACTGG ACTGGAGAAT GGAATCCTTC TGTGCGAGTT ATTAAATGCT 
ATTAAACCAG GACTCGTAAA AAAGATCAAC AGACTTCCTA CTCCCATTGC TGGATTGGAC 
AATATAACAT TATTCCTTAG AGGCTGCAAA GAGCTCGGGC TGAAAGAATC TCAGTTGTTT 
GACCCAGGTG ACCTACAGGA CACAGCAAAC AGGACAACAG CCAGGAATTC AGATTGCAAC 
AGAAAACTAA GAAATGTACT AATCACCGTG TACTGGCTGG GGAAAGCTGC AAGCAGCTGC 
TCGTCCTATA GTGGCAACAA CCTGGACTTA AAGGAGTTTG AGGGATTGCT GGCACAAATG 
AGAAAAGAAA ATGAGGAAAT AGAGAGTCCA AAACGCAGCA TACGGGACAG TGGCTACATA 
GATTGTTGGG ACTCTGAGAG AAGTGACTCC CTTTCTCCTC CCAGACATGG TAGAGATGAC 
TCCTTCGATA GTTTAGACTC ATTTGGATCT AGATCACAAC AGACACCATC TCCAGATGTA 
GTGCTGAGGG GAAGCAGCGA CGGCAGAGGA AGCGACTCTG AGTCTGACCT ACCTCACAGA 
AGGTTACCTG ATGTGCGAAA AGATGATATG TCTGCTAGAC GGGTATCCTA CGGTGAAAAC 
AAAGCATTTG TGCCTTTCAA CCAATACCTC CCCAACAAGA GCAACCAGAC CTCCTACATC 
CCGGCACCCC TGAGGAAGAA GAAGGCTGAG CGTGAGGACT ACCGGAAAAG CTGGAGCACA 
GCTACATCCC CCCTGGGGGG AGAGCGACCC TTCAGTCCCA AACAAAGTCC AGTCATTAAA 
GAAGAACAAA ACGAGTTGCA GTGTGAAGGA GAAGGGACCA AGCAGATGAT TGCCTTAAAA 
GAACCTTGCC TTGGAGCAGA AAATAGGAGT GAGAGAGTTG GTGAAAGTGA AGGGGGTGTC 
ATGGGACAAT CATCTGCTAA TGATGACATT CCAAAAGATG CAGAGGAAGT GAGAAAGCGC 
AAGCACCTTC AGAAAGCGGG TATCAAGGTC TTGCCCGCAA CAAAGCGCTA CAGCAGTCCA 
AGTCAATTTG CTGAAGACAT TGATGGCGCT CCAGACATTG TGCTGCGAAA AGAGAACACC 
TTCCTGAAGT CATATGAAGG GATGGGCTCT GATTCCGAAG AAGAAGAAGA GTGCAAAGTA 
CCTGATATAC AGCGAGATGA CTTGGCCACA AGGCGTGCAA GGATGAACCA ACCAAAAAGC 
CCAATGCAGT TTAATCAGTA TATACAGGGG TCATACTTAA ACAAAAACAG AGCATCAAGA 
AAGCTGAACA GTTCTAAGAC TTCCACTCCA GCTTCTCCAA AAATGTCTCA AGATATCAGG 
GAGTACCAAG GCCCCCAGTT ATTAAAGCCA TCACACGTCT GCAAAGAAAA GACATTTCCA 
ATACCCTCAG ACAATAAAGA GGATGCAGAA GCAGAGCAAG AAAAAGTTCC CGACATTCAG 
AAAGACGACC TGGCGAAACG CAAAGCTAAA GCTTCAGTTT CACTGCCAAA GGAACCATCG 
GCCTTTGCAG CGGTTTCAAT TACAAAGGCC GACATGGAAA CTTGGCAGAG GCTTCGAATG 
TCTTCTGAAG ATAGTGAGCA CTTGAATGAC AGGCAGGCTG ACCAGCAAGA GCAAACATCC 
AAGATTGATG TGACTAGAGC GACAAATGAT GACTTTGGGA GGAGGAAATT GTCTCTGCAA 
GAGAAAAGGG CTCGAAACAA ACAGAAGTTT GTACGCTTTG GCCCAGTGAC TGAAATTGAT 
CAGCAGAAGT GGGATAGGCT TTGTATAGCC AAGGCTGAAT CTGGCCAGGA AAGTGAAGAG 
ATGAAGGGCA GCATTACGTC TGATGAAACT GCCAGCCACA TATCTCCCTC AGCTTCTGCT 
CAGCGCTCCC AACCCCAGGA GGATGTTGGC ACAGGGCCAG TCTTGCCACA TGAGTCAAGA 
TCTTTTACAC CAGAGAGATT GGTTTCACCT GCTCCTCCTG AAAATGAAAG TGAAATTCAG 
CTCCTAGAAA CACAGCAAGA TGACATGATG GCAAGGAGAA CAGGTACATT CCAAAAATGC 
GCCAGAACCA GCAGAGACTC TCCTGTGTCC CACCAATATG CACTCCATAG CCAACAACAC 
GAAGAGAAGT CTGACTCGCT GGAACTCGAA GAGAAACAAG GACTCTTTCT GGATGTTAAC 
CCACAACTGA GAAAATTACT GCTGTCGGAC TTGGCAAATA CTAGTTCAAA AGCAACTAGC 
CCTACTTTGG GGAGCAAGTC TCTGAAAGTC ACCAAAGAAG CTGAAGATGT AAAAAATGAC 
ATGAAAGAAG GTGCCTTGAA CACAGGAAAG GATCCAAACT CTGTTATCCC AGAGGATAAC 
ACAATTGCAC CAGACCCAGA AAAAGATGAC ATGATGACAA GAAAAATCTC AGTGAGCCAG 
AAGCCATCTT GCGTCAGCCT TGGACACTTC CTTCCTGTTC CTTTTTCTTT GCAGCGCAGA 
GAGGGGGAAA AGAAACCAAC AAGCTGCATC ATATCAAGCA CCAAAAAGTC CAAAAATGCA 
TCCAAAGCTG TTCCAGCCGA CATTTCTGAT GTTCCTTCTA AGCCCCTTGA CCAGAAAGAA 
ACAAAAGAAC ATGGTGAATT AGGAGATACG ACTTGCAAGC AAGCCTCCAA TAGTGTGGAG 
ACTCCCATGT GTACAGAGTT AGACAGCCAG CAACAGGATG GTCTTGAGAG GAGAATGATA 
CTGTCTACTG ACTTGGCCAA TACTGAGTTA AAAATAACTC CTCCTACTTT GGCCAACAAC 
TCTCCAACCA TAACAAAAGT ACAAGAGCCA GATGCAACCA TCTCAGAGGA CAATACAACT 
GCACCAAACC CTGAAAAAGA TGATATGATG ACCAGAAGAA TCTCTGTAAC CCAGAAACCA 
TCGTGTGTAG GTCTTGGCCA CTTCCTTCCA GTTCCATTTT CTTTGCAGCA AAAAGTTGAG 
GAAAAGAAAT CAACGAGATT CAGCCCAAGA TCTGTTCTGT CTGATGATGG AGAGAGCATG 
AGCATGTGCG ACATGAGGTA CGAAGAAGAG GGAAGTATGC AGCCTCACAG CAGGGCACAC 
TATGAGCATC TACAGCATGT CAACAGCTTG CTCAAGGAGG AGGATGACAC ATGGCAAGAT 
GACCTAGCTA AGTGGAAGAC ACGGAGAAGA AGTGCATCAC AGGATCTGAT CAAAAAAGAG 
GAACAGAGGA AGAAGATGGA GAGAATGATG AGCGGAGAGG ATGAACTGTC GGAGCGTAGG 
AAAAGCATAA AAACATATAG AGAGATTGTG GAAGAAAAAG AACGTCGAGA GAAAGAACTG 
CATGAAGCCT ACAAAAATGC CAAGTCAAGA GATGAGGCTG AACACATATT ACAGAGCTAC 
ATTGAAAAGT TTACAATTAG TGAAGCTGTT CTTGAGGGAT TAGTAATGCC AAAAATCTTA 
GAAAGAAGCC AGTCGGTGGA GCCAAGTTTA TTGTCTTCAT CAGAGGACCC AAACATCCTG 
AAATACCTGA GGCAACAGTC TTTACCATCA CCAAAATACA CTTCCACTGT TGAAGCAACT 
GTCTCCCCCA GCAGCCCATG TGAAGCAAAA AGTCCAACAG GTCTGATATC ACCAAGCAAA 
AACATTATCT CAAAGACTGT GCCTATGCTA ACAGCCAAGC CTTACTCCCA GCCCAAAAAC 
ACCCAGGAAG TCCTCAAGAC ATTTAAGGTA GATGGAAAGG TAAACCTCAA CGGGGTAGAA 
GAGCAGAAAG AAGAGGAACA TGGAAACCAG ACATTTGCCC CATCACTCAC CCGATCTCAG 
ATGTTTGAAG GTGTAGCCAG AGTTGATGTT CCTGTTGTTG AAATAAAGCA AGACGTAACT 
TCCATAGAGC TTAATCTGGC CAGACCAAAC ACCCTGAATT CCTTACAAGA ACCCAAAAGT 
CTCTGCGAAG ACAGAAATCA CATAAGCCAG GAGGATGAGT CAGATTCTCT GGTGCAATTA 
GAAAGAGCTC ACTCCGTGGA AATATTGTCA GAAGGTAACA CCCAATCAAG CAATGCAACC 
CAGGATGTAC CTGTTGTGAG CAGTATAGAG ATTCCACCAT TGACCATCAA TGGAAAGGAC 
ACTGTGGATG AGAGGAAAGT GGAAGCTGCC GAGTTGATCA TGCATTGGTC CCTGGAGCCG 
AATCATAAGG AAGCCAAAGA AAATTCTTTA TCGCCTTTAA AAACTTCTGA AAATTTAGAA 
GAATCCTTGC AGTTTAAAGA GAATGAATCA TCACACAAAG TGCAGCTAAA CATTACGCTT 
CAGAGCCGGA ACGCCTGGAG ACGCTCCCAG TTTTTTTTGC AGCCAGTGGA TACAGAATGT 
ACAGAAAATT CACCAGCACT TGTACCCAGT TTGAATTCTC CTAACCGATT CTGCACATGG 
GACCCTGAAG AAGAACGCAA ACGGCAAGAA AAATGGCAGC AGGAGCAGGA AAGGCTTCTC 
CAGGAGAGGT ACCAGAGAGA GCAGGAAAAA CTTAAAGAAG AATGGGAAAA AGCTCAGAAA 
GAAGTAGAAG AGGAGGAAAG GAAATATTAT GAGGAGGAAA GAAAGATAAT TGAAGACACT 
GTGGTCCCAC TGACTTTGTC TCCAAATTCT AATGCACAGT TTAATATCTC TGAAAGAAAT 
GGGAACTTCA TTCCAGCACT TACAGAACAT GTCAAGAGAG ATCAAGAAAA TAACAATGAA 
AAGCTCTTGA CAGAGTCCAG CAGAACGATA AAGAATGAGA CACATATGCA CAGCAATGTA 
ACACAGCAAC TGCTTTTGCA TACACAGGAG GGGTCAATTC TTGGTGCTAA AGAGAAACAA 
ATAAAGGGAA AAACAGACAA GTGGGAAGCA GAACAGTCCA TCTGTATTAT GGACCGAGAG 
GAGCTGAGCA ACCATGTGCA GTCTAATGAA TGTAATACAC CCACTAGGTT ACCCTTGCAG 
GCAGGATGTA AACAAGTGAC GTCATCAGAG TCAAGTCTGC CTGGGGATCT TGGATCACCA 
AAAAATCCTA TCACTGAAAA AGATGCTATT CAGAATATGT CGTCCATAAT ACCCACACGG 
GGAACTGATT TCAAGGAAGA TACAATGAAT TCTTCACAGT CCTCTACATC ACAGTGCCAG 
TCACCAAACA GGTCTGTTAG TGGGAAAAAA CTGTGCTCCA CATGTGGACT TCCTCTAGGC 
AAGGGAGCTG CCATGATAAT TGAAACCCTC AGTCTTTATT TCCACATCCA GTGCTTCAAG 
TGTGGACTGT GTAAGGGACA ACTTGGGGAT GCGATCACCG GCACAGACGT CAGAATTCGA 
AATGGCCTAC TGAACTGCAA TGACTGTTAC ATGAAGTCTA GAAGTAAGGC GGCTGGCCAC 
CCCACCCCTT TGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031751245.1
CDS	491..6025
Translation	MASSATLQGEEIPPPQESPPDTACREAQKWIEQVTGKSFGDKDFRTGLENGILLCELLNAIKPGLVKKINRLPTPIAGLDNITLFLRGCKELGLKESQLFDPGDLQDTANRTTARNSDCNRKLRNVLITVYWLGKAASSCSSYSGNNLDLKEFEGLLAQMRKENEEIESPKRSIRDSGYIDCWDSERSDSLSPPRHGRDDSFDSLDSFGSRSQQTPSPDVVLRGSSDGRGSDSESDLPHRRLPDVRKDDMSARRVSYGENKAFVPFNQYLPNKSNQTSYIPAPLRKKKAEREDYRKSWSTATSPLGGERPFSPKQSPVIKEEQNELQCEGEGTKQMIALKEPCLGAENRSERVGESEGGVMGQSSANDDIPKDAEEVRKRKHLQKAGIKVLPATKRYSSPSQFAEDIDGAPDIVLRKENTFLKSYEGMGSDSEEEEECKVPDIQRDDLATRRARMNQPKSPMQFNQYIQGSYLNKNRASRKLNSSKTSTPASPKMSQDIREYQGPQLLKPSHVCKEKTFPIPSDNKEDAEAEQEKVPDIQKDDLAKRKAKASVSLPKEPSAFAAVSITKADMETWQRLRMSSEDSEHLNDRQADQQEQTSKIDVTRATNDDFGRRKLSLQEKRARNKQKFVRFGPVTEIDQQKWDRLCIAKAESGQESEEMKGSITSDETASHISPSASAQRSQPQEDVGTGPVLPHESRSFTPERLVSPAPPENESEIQLLETQQDDMMARRTGTFQKCARTSRDSPVSHQYALHSQQHEEKSDSLELEEKQGLFLDVNPQLRKLLLSDLANTSSKATSPTLGSKSLKVTKEAEDVKNDMKEGALNTGKDPNSVIPEDNTIAPDPEKDDMMTRKISVSQKPSCVSLGHFLPVPFSLQRREGEKKPTSCIISSTKKSKNASKAVPADISDVPSKPLDQKETKEHGELGDTTCKQASNSVETPMCTELDSQQQDGLERRMILSTDLANTELKITPPTLANNSPTITKVQEPDATISEDNTTAPNPEKDDMMTRRISVTQKPSCVGLGHFLPVPFSLQQKVEEKKSTRFSPRSVLSDDGESMSMCDMRYEEEGSMQPHSRAHYEHLQHVNSLLKEEDDTWQDDLAKWKTRRRSASQDLIKKEEQRKKMERMMSGEDELSERRKSIKTYREIVEEKERREKELHEAYKNAKSRDEAEHILQSYIEKFTISEAVLEGLVMPKILERSQSVEPSLLSSSEDPNILKYLRQQSLPSPKYTSTVEATVSPSSPCEAKSPTGLISPSKNIISKTVPMLTAKPYSQPKNTQEVLKTFKVDGKVNLNGVEEQKEEEHGNQTFAPSLTRSQMFEGVARVDVPVVEIKQDVTSIELNLARPNTLNSLQEPKSLCEDRNHISQEDESDSLVQLERAHSVEILSEGNTQSSNATQDVPVVSSIEIPPLTINGKDTVDERKVEAAELIMHWSLEPNHKEAKENSLSPLKTSENLEESLQFKENESSHKVQLNITLQSRNAWRRSQFFLQPVDTECTENSPALVPSLNSPNRFCTWDPEEERKRQEKWQQEQERLLQERYQREQEKLKEEWEKAQKEVEEEERKYYEEERKIIEDTVVPLTLSPNSNAQFNISERNGNFIPALTEHVKRDQENNNEKLLTESSRTIKNETHMHSNVTQQLLLHTQEGSILGAKEKQIKGKTDKWEAEQSICIMDREELSNHVQSNECNTPTRLPLQAGCKQVTSSESSLPGDLGSPKNPITEKDAIQNMSSIIPTRGTDFKEDTMNSSQSSTSQCQSPNRSVSGKKLCSTCGLPLGKGAAMIIETLSLYFHIQCFKCGLCKGQLGDAITGTDVRIRNGLLNCNDCYMKSRSKAAGHPTPL

Target ORF information:

RefSeq Version	XM_031895385.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis LIM and calponin homology domains 1 (limch1), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031895385.1

ORF Insert Sequence:

ATGGCCAGCT CCGCCACACT GCAAGGGGAA GAGATCCCCC CGCCCCAGGA GTCTCCCCCA 
GACACGGCTT GCAGGGAGGC ACAGAAGTGG ATTGAGCAAG TTACTGGCAA AAGCTTTGGC 
GACAAAGACT TCAGGACTGG ACTGGAGAAT GGAATCCTTC TGTGCGAGTT ATTAAATGCT 
ATTAAACCAG GACTCGTAAA AAAGATCAAC AGACTTCCTA CTCCCATTGC TGGATTGGAC 
AATATAACAT TATTCCTTAG AGGCTGCAAA GAGCTCGGGC TGAAAGAATC TCAGTTGTTT 
GACCCAGGTG ACCTACAGGA CACAGCAAAC AGGACAACAG CCAGGAATTC AGATTGCAAC 
AGAAAACTAA GAAATGTACT AATCACCGTG TACTGGCTGG GGAAAGCTGC AAGCAGCTGC 
TCGTCCTATA GTGGCAACAA CCTGGACTTA AAGGAGTTTG AGGGATTGCT GGCACAAATG 
AGAAAAGAAA ATGAGGAAAT AGAGAGTCCA AAACGCAGCA TACGGGACAG TGGCTACATA 
GATTGTTGGG ACTCTGAGAG AAGTGACTCC CTTTCTCCTC CCAGACATGG TAGAGATGAC 
TCCTTCGATA GTTTAGACTC ATTTGGATCT AGATCACAAC AGACACCATC TCCAGATGTA 
GTGCTGAGGG GAAGCAGCGA CGGCAGAGGA AGCGACTCTG AGTCTGACCT ACCTCACAGA 
AGGTTACCTG ATGTGCGAAA AGATGATATG TCTGCTAGAC GGGTATCCTA CGGTGAAAAC 
AAAGCATTTG TGCCTTTCAA CCAATACCTC CCCAACAAGA GCAACCAGAC CTCCTACATC 
CCGGCACCCC TGAGGAAGAA GAAGGCTGAG CGTGAGGACT ACCGGAAAAG CTGGAGCACA 
GCTACATCCC CCCTGGGGGG AGAGCGACCC TTCAGTCCCA AACAAAGTCC AGTCATTAAA 
GAAGAACAAA ACGAGTTGCA GTGTGAAGGA GAAGGGACCA AGCAGATGAT TGCCTTAAAA 
GAACCTTGCC TTGGAGCAGA AAATAGGAGT GAGAGAGTTG GTGAAAGTGA AGGGGGTGTC 
ATGGGACAAT CATCTGCTAA TGATGACATT CCAAAAGATG CAGAGGAAGT GAGAAAGCGC 
AAGCACCTTC AGAAAGCGGG TATCAAGGTC TTGCCCGCAA CAAAGCGCTA CAGCAGTCCA 
AGTCAATTTG CTGAAGACAT TGATGGCGCT CCAGACATTG TGCTGCGAAA AGAGAACACC 
TTCCTGAAGT CATATGAAGG GATGGGCTCT GATTCCGAAG AAGAAGAAGA GTGCAAAGTA 
CCTGATATAC AGCGAGATGA CTTGGCCACA AGGCGTGCAA GGATGAACCA ACCAAAAAGC 
CCAATGCAGT TTAATCAGTA TATACAGGGG TCATACTTAA ACAAAAACAG AGCATCAAGA 
AAGCTGAACA GTTCTAAGAC TTCCACTCCA GCTTCTCCAA AAATGTCTCA AGATATCAGG 
GAGTACCAAG GCCCCCAGTT ATTAAAGCCA TCACACGTCT GCAAAGAAAA GACATTTCCA 
ATACCCTCAG ACAATAAAGA GGATGCAGAA GCAGAGCAAG AAAAAGTTCC CGACATTCAG 
AAAGACGACC TGGCGAAACG CAAAGCTAAA GCTTCAGTTT CACTGCCAAA GGAACCATCG 
GCCTTTGCAG CGGTTTCAAT TACAAAGGCC GACATGGAAA CTTGGCAGAG GCTTCGAATG 
TCTTCTGAAG ATAGTGAGCA CTTGAATGAC AGGCAGGCTG ACCAGCAAGA GCAAACATCC 
AAGATTGATG TGACTAGAGC GACAAATGAT GACTTTGGGA GGAGGAAATT GTCTCTGCAA 
GAGAAAAGGG CTCGAAACAA ACAGAAGTTT GTACGCTTTG GCCCAGTGAC TGAAATTGAT 
CAGCAGAAGT GGGATAGGCT TTGTATAGCC AAGGCTGAAT CTGGCCAGGA AAGTGAAGAG 
ATGAAGGGCA GCATTACGTC TGATGAAACT GCCAGCCACA TATCTCCCTC AGCTTCTGCT 
CAGCGCTCCC AACCCCAGGA GGATGTTGGC ACAGGGCCAG TCTTGCCACA TGAGTCAAGA 
TCTTTTACAC CAGAGAGATT GGTTTCACCT GCTCCTCCTG AAAATGAAAG TGAAATTCAG 
CTCCTAGAAA CACAGCAAGA TGACATGATG GCAAGGAGAA CAGGTACATT CCAAAAATGC 
GCCAGAACCA GCAGAGACTC TCCTGTGTCC CACCAATATG CACTCCATAG CCAACAACAC 
GAAGAGAAGT CTGACTCGCT GGAACTCGAA GAGAAACAAG GACTCTTTCT GGATGTTAAC 
CCACAACTGA GAAAATTACT GCTGTCGGAC TTGGCAAATA CTAGTTCAAA AGCAACTAGC 
CCTACTTTGG GGAGCAAGTC TCTGAAAGTC ACCAAAGAAG CTGAAGATGT AAAAAATGAC 
ATGAAAGAAG GTGCCTTGAA CACAGGAAAG GATCCAAACT CTGTTATCCC AGAGGATAAC 
ACAATTGCAC CAGACCCAGA AAAAGATGAC ATGATGACAA GAAAAATCTC AGTGAGCCAG 
AAGCCATCTT GCGTCAGCCT TGGACACTTC CTTCCTGTTC CTTTTTCTTT GCAGCGCAGA 
GAGGGGGAAA AGAAACCAAC AAGCTGCATC ATATCAAGCA CCAAAAAGTC CAAAAATGCA 
TCCAAAGCTG TTCCAGCCGA CATTTCTGAT GTTCCTTCTA AGCCCCTTGA CCAGAAAGAA 
ACAAAAGAAC ATGGTGAATT AGGAGATACG ACTTGCAAGC AAGCCTCCAA TAGTGTGGAG 
ACTCCCATGT GTACAGAGTT AGACAGCCAG CAACAGGATG GTCTTGAGAG GAGAATGATA 
CTGTCTACTG ACTTGGCCAA TACTGAGTTA AAAATAACTC CTCCTACTTT GGCCAACAAC 
TCTCCAACCA TAACAAAAGT ACAAGAGCCA GATGCAACCA TCTCAGAGGA CAATACAACT 
GCACCAAACC CTGAAAAAGA TGATATGATG ACCAGAAGAA TCTCTGTAAC CCAGAAACCA 
TCGTGTGTAG GTCTTGGCCA CTTCCTTCCA GTTCCATTTT CTTTGCAGCA AAAAGTTGAG 
GAAAAGAAAT CAACGAGATT CAGCCCAAGA TCTGTTCTGT CTGATGATGG AGAGAGCATG 
AGCATGTGCG ACATGAGGTA CGAAGAAGAG GGAAGTATGC AGCCTCACAG CAGGGCACAC 
TATGAGCATC TACAGCATGT CAACAGCTTG CTCAAGGAGG AGGATGACAC ATGGCAAGAT 
GACCTAGCTA AGTGGAAGAC ACGGAGAAGA AGTGCATCAC AGGATCTGAT CAAAAAAGAG 
GAACAGAGGA AGAAGATGGA GAGAATGATG AGCGGAGAGG ATGAACTGTC GGAGCGTAGG 
AAAAGCATAA AAACATATAG AGAGATTGTG GAAGAAAAAG AACGTCGAGA GAAAGAACTG 
CATGAAGCCT ACAAAAATGC CAAGTCAAGA GATGAGGCTG AACACATATT ACAGAGCTAC 
ATTGAAAAGT TTACAATTAG TGAAGCTGTT CTTGAGGGAT TAGTAATGCC AAAAATCTTA 
GAAAGAAGCC AGTCGGTGGA GCCAAGTTTA TTGTCTTCAT CAGAGGACCC AAACATCCTG 
AAATACCTGA GGCAACAGTC TTTACCATCA CCAAAATACA CTTCCACTGT TGAAGCAACT 
GTCTCCCCCA GCAGCCCATG TGAAGCAAAA AGTCCAACAG GTCTGATATC ACCAAGCAAA 
AACATTATCT CAAAGACTGT GCCTATGCTA ACAGCCAAGC CTTACTCCCA GCCCAAAAAC 
ACCCAGGAAG TCCTCAAGAC ATTTAAGGTA GATGGAAAGG TAAACCTCAA CGGGGTAGAA 
GAGCAGAAAG AAGAGGAACA TGGAAACCAG ACATTTGCCC CATCACTCAC CCGATCTCAG 
ATGTTTGAAG GTGTAGCCAG AGTTGATGTT CCTGTTGTTG AAATAAAGCA AGACGTAACT 
TCCATAGAGC TTAATCTGGC CAGACCAAAC ACCCTGAATT CCTTACAAGA ACCCAAAAGT 
CTCTGCGAAG ACAGAAATCA CATAAGCCAG GAGGATGAGT CAGATTCTCT GGTGCAATTA 
GAAAGAGCTC ACTCCGTGGA AATATTGTCA GAAGGTAACA CCCAATCAAG CAATGCAACC 
CAGGATGTAC CTGTTGTGAG CAGTATAGAG ATTCCACCAT TGACCATCAA TGGAAAGGAC 
ACTGTGGATG AGAGGAAAGT GGAAGCTGCC GAGTTGATCA TGCATTGGTC CCTGGAGCCG 
AATCATAAGG AAGCCAAAGA AAATTCTTTA TCGCCTTTAA AAACTTCTGA AAATTTAGAA 
GAATCCTTGC AGTTTAAAGA GAATGAATCA TCACACAAAG TGCAGCTAAA CATTACGCTT 
CAGAGCCGGA ACGCCTGGAG ACGCTCCCAG TTTTTTTTGC AGCCAGTGGA TACAGAATGT 
ACAGAAAATT CACCAGCACT TGTACCCAGT TTGAATTCTC CTAACCGATT CTGCACATGG 
GACCCTGAAG AAGAACGCAA ACGGCAAGAA AAATGGCAGC AGGAGCAGGA AAGGCTTCTC 
CAGGAGAGGT ACCAGAGAGA GCAGGAAAAA CTTAAAGAAG AATGGGAAAA AGCTCAGAAA 
GAAGTAGAAG AGGAGGAAAG GAAATATTAT GAGGAGGAAA GAAAGATAAT TGAAGACACT 
GTGGTCCCAC TGACTTTGTC TCCAAATTCT AATGCACAGT TTAATATCTC TGAAAGAAAT 
GGGAACTTCA TTCCAGCACT TACAGAACAT GTCAAGAGAG ATCAAGAAAA TAACAATGAA 
AAGCTCTTGA CAGAGTCCAG CAGAACGATA AAGAATGAGA CACATATGCA CAGCAATGTA 
ACACAGCAAC TGCTTTTGCA TACACAGGAG GGGTCAATTC TTGGTGCTAA AGAGAAACAA 
ATAAAGGGAA AAACAGACAA GTGGGAAGCA GAACAGTCCA TCTGTATTAT GGACCGAGAG 
GAGCTGAGCA ACCATGTGCA GTCTAATGAA TGTAATACAC CCACTAGGTT ACCCTTGCAG 
GCAGGATGTA AACAAGTGAC GTCATCAGAG TCAAGTCTGC CTGGGGATCT TGGATCACCA 
AAAAATCCTA TCACTGAAAA AGATGCTATT CAGAATATGT CGTCCATAAT ACCCACACGG 
GGAACTGATT TCAAGGAAGA TACAATGAAT TCTTCACAGT CCTCTACATC ACAGTGCCAG 
TCACCAAACA GGTCTGTTAG TGGGAAAAAA CTGTGCTCCA CATGTGGACT TCCTCTAGGC 
AAGGGAGCTG CCATGATAAT TGAAACCCTC AGTCTTTATT TCCACATCCA GTGCTTCAAG 
TGTGGACTGT GTAAGGGACA ACTTGGGGAT GCGATCACCG GCACAGACGT CAGAATTCGA 
AATGGCCTAC TGAACTGCAA TGACTGTTAC ATGAAGTCTA GAAGTAAGGC GGCTGGCCAC 
CCCACCCCTT TGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

limch1 ( XM_012955699.3 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012811153.2 Xenopus tropicalis LIM and calponin homology domains 1 (limch1), transcript variant X8, mRNA.

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CloneID	OXa49701
Clone ID Related Accession (Same CDS sequence)	XM_012955699.3
Accession Version	XM_012955699.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5508bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1576512000000
Organism	Xenopus tropicalis(tropical clawed frog)
Product	LIM and calponin homology domains-containing protein 1 isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012955699.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCAGCT CCGCCACACT GCAAGGGGAA GAGATCCCCC CGCCCCAGGA GTCTCCCCCA 
GACACGGCTT GCAGGGAGGC ACAGAAGTGG ATTGAGCAAG TTACTGGCAA AAGCTTTGGC 
GACAAAGACT TCAGGACTGG ACTGGAGAAT GGAATCCTTC TGTGCGAGTT ATTAAATGCT 
ATTAAACCAG GACTCGTAAA AAAGATCAAC AGACTTCCTA CTCCCATTGC TGGATTGGAC 
AATATAACAT TATTCCTTAG AGGCTGCAAA GAGCTCGGGC TGAAAGAATC TCAGTTGTTT 
GACCCAGGTG ACCTACAGGA CACAGCAAAC AGGACAACAG CCAGGAATTC AGATTGCAAC 
AGAAAACTAA GAAATGTACT AATCACCGTG TACTGGCTGG GGAAAGCTGC AAGCAGCTGC 
TCGTCCTATA GTGGCAACAA CCTGGACTTA AAGGAGTTTG AGGGATTGCT GGCACAAATG 
AGAAAAGAAA ATGAGGAAAT AGAGAGTCCA AAACGCAGCA TACGGGACAG TGGCTACATA 
GATTGTTGGG ACTCTGAGAG AAGTGACTCC CTTTCTCCTC CCAGACATGG TAGAGATGAC 
TCCTTCGATA GTTTAGACTC ATTTGGATCT AGATCACAAC AGACACCATC TCCAGATGTA 
GTGCTGAGGG GAAGCAGCGA CGGCAGAGGA AGCGACTCTG AGTCTGACCT ACCTCACAGA 
AGGTTACCTG ATGTGCGAAA AGATGATATG TCTGCTAGAC GGGTATCCTA CGGTGAAAAC 
AAAGCATTTG TGCCTTTCAA CCAATACCTC CCCAACAAGA GCAACCAGAC CTCCTACATC 
CCGGCACCCC TGAGGAAGAA GAAGGCTGAG CGTGAGGACT ACCGGAAAAG CTGGAGCACA 
GCTACATCCC CCCTGGGGGG AGAGCGACCC TTCAGTCCCA AACAAAGTCC AGTCATTAAA 
GAAGAACAAA ACGAGTTGCA GTGTGAAGGA GAAGGGACCA AGCAGATGAT TGCCTTAAAA 
GAACCTTGCC TTGGAGCAGA AAATAGGAGT GAGAGAGTTG GTGAAAGTGA AGGGGGTGTC 
ATGGGACAAT CATCTGCTAA TGATGACATT CCAAAAGATG CAGAGGAAGT GAGAAAGCGC 
AAGCACCTTC AGAAAGCGGG TATCAAGGTC TTGCCCGCAA CAAAGCGCTA CAGCAGTCCA 
AGTCAATTTG CTGAAGACAT TGATGGCGCT CCAGACATTG TGCTGCGAAA AGAGAACACC 
TTCCTGAAGT CATATGAAGG GATGGGCTCT GATTCCGAAG AAGAAGAAGA GTGCAAAGTA 
CCTGATATAC AGCGAGATGA CTTGGCCACA AGGCGTGCAA GGATGAACCA ACCAAAAAGC 
CCAATGCAGT TTAATCAGTA TATACAGGGG TCATACTTAA ACAAAAACAG AGCATCAAGA 
AAGCTGAACA GTTCTAAGAC TTCCACTCCA GCTTCTCCAA AAATGTCTCA AGATATCAGG 
GAGTACCAAG GCCCCCAGTT ATTAAAGCCA TCACACGTCT GCAAAGAAAA GACATTTCCA 
ATACCCTCAG ACAATAAAGA GGATGCAGAA GCAGAGCAAG AAAAAGTTCC CGACATTCAG 
AAAGACGACC TGGCGAAACG CAAAGCTAAA GCTTCAGTTT CACTGCCAAA GGAACCATCG 
GCCTTTGCAG CGGTTTCAAT TACAAAGGCC GACATGGAAA CTTGGCAGAG GCTTCGAATG 
TCTTCTGAAG ATAGTGAGCA CTTGAATGAC AGGCAGGCTG ACCAGCAAGA GCAAACATCC 
AAGATTGATG TGACTAGAGC GACAAATGAT GACTTTGGGA GGAGGAAATT GTCTCTGCAA 
GAGAAAAGGG CTCGAAACAA ACAGAAGTTT GTACGCTTTG GCCCAGTGAC TGAAATTGAT 
CAGCAGAAGT GGGATAGGCT TTGTATAGCC AAGGCTGAAT CTGGCCAGGA AAGTGAAGAG 
ATGAAGGGCA GCATTACGTC TGATGAAACT GCCAGCCACA TATCTCCCTC AGCTTCTGCT 
CAGCGCTCCC AACCCCAGGA GGATGTTGGC ACAGGGCCAG TCTTGCCACA TGAGTCAAGA 
TCTTTTACAC CAGAGAGATT GGTTTCACCT GCTCCTCCTG AAAATGAAAG TGAAATTCAG 
CTCCTAGAAA CACAGCAAGA TGACATGATG GCAAGGAGAA CAGGTACATT CCAAAAATGC 
GCCAGAACCA GCAGAGACTC TCCTGTGTCC CACCAATATG CACTCCATAG CCAACAACAC 
GAAGAGAAGT CTGACTCGCT GGAACTCGAA GAGAAACAAG GACTCTTTCT GGATGTTAAC 
CCACAACTGA GAAAATTACT GCTGTCGGAC TTGGCAAATA CTAGTTCAAA AGCAACTAGC 
CCTACTTTGG GGAGCAAGTC TCTGAAAGTC ACCAAAGAAG CTGAAGATGT AAAAAATGAC 
ATGAAAGAAG GTGCCTTGAA CACAGGAAAG GATCCAAACT CTGTTATCCC AGAGGATAAC 
ACAATTGCAC CAGACCCAGA AAAAGATGAC ATGATGACAA GAAAAATCTC AGTGAGCCAG 
AAGCCATCTT GCGTCAGCCT TGGACACTTC CTTCCTGTTC CTTTTTCTTT GCAGCGCAGA 
GAGGGGGAAA AGAAACCAAC AAGCTGCATC ATATCAAGCA CCAAAAAGTC CAAAAATGCA 
TCCAAAGCTG TTCCAGCCGA CATTTCTGAT GTTCCTTCTA AGCCCCTTGA CCAGAAAGAA 
ACAAAAGAAC ATGGTGAATT AGGAGATACG ACTTGCAAGC AAGCCTCCAA TAGTGTGGAG 
ACTCCCATGT GTACAGAGTT AGACAGCCAG CAACAGGATG GTCTTGAGAG CTCCACTCTA 
TCGATCAAAA TAAAGTCAGA TAATTGCACC CAAGCACTTG CAGTCGGCAG TTCTGACCTT 
CCTTGTAAGC TCCTCGGCCA ATGTGAAACA GAGGGACATG TTGAATTACG ATCCGATGTG 
ACTTGCGATC AAACCTCCAC TGAGATCATT AGTGATGGGA TTCCCACTGG TAAAGGGTTA 
GATACCCAGG CAGAAGACGC TCTTGAGACA TGTGACCATG AGTTATTCAG ATTCAGCCCA 
AGATCTGTTC TGTCTGATGA TGGAGAGAGC ATGAGCATGT GCGACATGAG GTACGAAGAA 
GAGGGAAGTA TGCAGCCTCA CAGCAGGGCA CACTATGAGC ATCTACAGCA TGTCAACAGC 
TTGCTCAAGG AGGAGGATGA CACATGGCAA GATGACCTAG CTAAGTGGAA GACACGGAGA 
AGAAGTGCAT CACAGGATCT GATCAAAAAA GAGGAACAGA GGAAGAAGAT GGAGAGAATG 
ATGAGCGGAG AGGATGAACT GTCGGAGCGT AGGAAAAGCA TAAAAACATA TAGAGAGATT 
GTGGAAGAAA AAGAACGTCG AGAGAAAGAA CTGCATGAAG CCTACAAAAA TGCCAAGTCA 
AGAGATGAGG CTGAACACAT ATTACAGAGC TACATTGAAA AGTTTACAAT TAGTGAAGCT 
GTTCTTGAGG GATTAGTAAT GCCAAAAATC TTAGAAAGAA GCCAGTCGGT GGAGCCAAGT 
TTATTGTCTT CATCAGAGGA CCCAAACATC CTGAAATACC TGAGGCAACA GTCTTTACCA 
TCACCAAAAT ACACTTCCAC TGTTGAAGCA ACTGTCTCCC CCAGCAGCCC ATGTGAAGCA 
AAAAGTCCAA CAGGTCTGAT ATCACCAAGC AAAAACATTA TCTCAAAGAC TGTGCCTATG 
CTAACAGCCA AGCCTTACTC CCAGCCCAAA AACACCCAGG AAGTCCTCAA GACATTTAAG 
GTAGATGGAA AGGTAAACCT CAACGGGGTA GAAGAGCAGA AAGAAGAGGA ACATGGAAAC 
CAGACATTTG CCCCATCACT CACCCGATCT CAGATGTTTG AAGGTGTAGC CAGAGTTGAT 
GTTCCTGTTG TTGAAATAAA GCAAGACGTA ACTTCCATAG AGCTTAATCT GGCCAGACCA 
AACACCCTGA ATTCCTTACA AGAACCCAAA AGTCTCTGCG AAGACAGAAA TCACATAAGC 
CAGGAGGATG AGTCAGATTC TCTGGTGCAA TTAGAAAGAG CTCACTCCGT GGAAATATTG 
TCAGAAGGTA ACACCCAATC AAGCAATGCA ACCCAGGATG TACCTGTTGT GAGCAGTATA 
GAGATTCCAC CATTGACCAT CAATGGAAAG GACACTGTGG ATGAGAGGAA AGTGGAAGCT 
GCCGAGTTGA TCATGCATTG GTCCCTGGAG CCGAATCATA AGGAAGCCAA AGAAAATTCT 
TTATCGCCTT TAAAAACTTC TGAAAATTTA GAAGAATCCT TGCAGTTTAA AGAGAATGAA 
TCATCACACA AAGTGCAGCT AAACATTACG CTTCAGAGCC GGAACGCCTG GAGACGCTCC 
CAGTTTTTTT TGCAGCCAGT GGATACAGAA TGTACAGAAA ATTCACCAGC ACTTGTACCC 
AGTTTGAATT CTCCTAACCG ATTCTGCACA TGGGACCCTG AAGAAGAACG CAAACGGCAA 
GAAAAATGGC AGCAGGAGCA GGAAAGGCTT CTCCAGGAGA GGTACCAGAG AGAGCAGGAA 
AAACTTAAAG AAGAATGGGA AAAAGCTCAG AAAGAAGTAG AAGAGGAGGA AAGGAAATAT 
TATGAGGAGG AAAGAAAGAT AATTGAAGAC ACTGTGGTCC CACTGACTTT GTCTCCAAAT 
TCTAATGCAC AGTTTAATAT CTCTGAAAGA AATGGGAACT TCATTCCAGC ACTTACAGAA 
CATGTCAAGA GAGATCAAGA AAATAACAAT GAAAAGCTCT TGACAGAGTC CAGCAGAACG 
ATAAAGAATG AGACACATAT GCACAGCAAT GTAACACAGC AACTGCTTTT GCATACACAG 
GAGGGGTCAA TTCTTGGTGC TAAAGAGAAA CAAATAAAGG GAAAAACAGA CAAGTGGGAA 
GCAGAACAGT CCATCTGTAT TATGGACCGA GAGGAGCTGA GCAACCATGT GCAGTCTAAT 
GAATGTAATA CACCCACTAG GTTACCCTTG CAGGCAGGAT GTAAACAAGT GACGTCATCA 
GAGTCAAGTC TGCCTGGGGA TCTTGGATCA CCAAAAAATC CTATCACTGA AAAAGATGCT 
ATTCAGAATA TGTCGTCCAT AATACCCACA CGGGGAACTG ATTTCAAGGA AGATACAATG 
AATTCTTCAC AGTCCTCTAC ATCACAGTGC CAGTCACCAA ACAGGTCTGT TAGTGGGAAA 
AAACTGTGCT CCACATGTGG ACTTCCTCTA GGCAAGGGAG CTGCCATGAT AATTGAAACC 
CTCAGTCTTT ATTTCCACAT CCAGTGCTTC AAGTGTGGAC TGTGTAAGGG ACAACTTGGG 
GATGCGATCA CCGGCACAGA CGTCAGAATT CGAAATGGCC TACTGAACTG CAATGACTGT 
TACATGAAGT CTAGAAGTAA GGCGGCTGGC CACCCCACCC CTTTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012811153.2
CDS	491..5998
Translation	MASSATLQGEEIPPPQESPPDTACREAQKWIEQVTGKSFGDKDFRTGLENGILLCELLNAIKPGLVKKINRLPTPIAGLDNITLFLRGCKELGLKESQLFDPGDLQDTANRTTARNSDCNRKLRNVLITVYWLGKAASSCSSYSGNNLDLKEFEGLLAQMRKENEEIESPKRSIRDSGYIDCWDSERSDSLSPPRHGRDDSFDSLDSFGSRSQQTPSPDVVLRGSSDGRGSDSESDLPHRRLPDVRKDDMSARRVSYGENKAFVPFNQYLPNKSNQTSYIPAPLRKKKAEREDYRKSWSTATSPLGGERPFSPKQSPVIKEEQNELQCEGEGTKQMIALKEPCLGAENRSERVGESEGGVMGQSSANDDIPKDAEEVRKRKHLQKAGIKVLPATKRYSSPSQFAEDIDGAPDIVLRKENTFLKSYEGMGSDSEEEEECKVPDIQRDDLATRRARMNQPKSPMQFNQYIQGSYLNKNRASRKLNSSKTSTPASPKMSQDIREYQGPQLLKPSHVCKEKTFPIPSDNKEDAEAEQEKVPDIQKDDLAKRKAKASVSLPKEPSAFAAVSITKADMETWQRLRMSSEDSEHLNDRQADQQEQTSKIDVTRATNDDFGRRKLSLQEKRARNKQKFVRFGPVTEIDQQKWDRLCIAKAESGQESEEMKGSITSDETASHISPSASAQRSQPQEDVGTGPVLPHESRSFTPERLVSPAPPENESEIQLLETQQDDMMARRTGTFQKCARTSRDSPVSHQYALHSQQHEEKSDSLELEEKQGLFLDVNPQLRKLLLSDLANTSSKATSPTLGSKSLKVTKEAEDVKNDMKEGALNTGKDPNSVIPEDNTIAPDPEKDDMMTRKISVSQKPSCVSLGHFLPVPFSLQRREGEKKPTSCIISSTKKSKNASKAVPADISDVPSKPLDQKETKEHGELGDTTCKQASNSVETPMCTELDSQQQDGLESSTLSIKIKSDNCTQALAVGSSDLPCKLLGQCETEGHVELRSDVTCDQTSTEIISDGIPTGKGLDTQAEDALETCDHELFRFSPRSVLSDDGESMSMCDMRYEEEGSMQPHSRAHYEHLQHVNSLLKEEDDTWQDDLAKWKTRRRSASQDLIKKEEQRKKMERMMSGEDELSERRKSIKTYREIVEEKERREKELHEAYKNAKSRDEAEHILQSYIEKFTISEAVLEGLVMPKILERSQSVEPSLLSSSEDPNILKYLRQQSLPSPKYTSTVEATVSPSSPCEAKSPTGLISPSKNIISKTVPMLTAKPYSQPKNTQEVLKTFKVDGKVNLNGVEEQKEEEHGNQTFAPSLTRSQMFEGVARVDVPVVEIKQDVTSIELNLARPNTLNSLQEPKSLCEDRNHISQEDESDSLVQLERAHSVEILSEGNTQSSNATQDVPVVSSIEIPPLTINGKDTVDERKVEAAELIMHWSLEPNHKEAKENSLSPLKTSENLEESLQFKENESSHKVQLNITLQSRNAWRRSQFFLQPVDTECTENSPALVPSLNSPNRFCTWDPEEERKRQEKWQQEQERLLQERYQREQEKLKEEWEKAQKEVEEEERKYYEEERKIIEDTVVPLTLSPNSNAQFNISERNGNFIPALTEHVKRDQENNNEKLLTESSRTIKNETHMHSNVTQQLLLHTQEGSILGAKEKQIKGKTDKWEAEQSICIMDREELSNHVQSNECNTPTRLPLQAGCKQVTSSESSLPGDLGSPKNPITEKDAIQNMSSIIPTRGTDFKEDTMNSSQSSTSQCQSPNRSVSGKKLCSTCGLPLGKGAAMIIETLSLYFHIQCFKCGLCKGQLGDAITGTDVRIRNGLLNCNDCYMKSRSKAAGHPTPL

Target ORF information:

RefSeq Version	XM_012955699.3
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis LIM and calponin homology domains 1 (limch1), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012955699.3

ORF Insert Sequence:

ATGGCCAGCT CCGCCACACT GCAAGGGGAA GAGATCCCCC CGCCCCAGGA GTCTCCCCCA 
GACACGGCTT GCAGGGAGGC ACAGAAGTGG ATTGAGCAAG TTACTGGCAA AAGCTTTGGC 
GACAAAGACT TCAGGACTGG ACTGGAGAAT GGAATCCTTC TGTGCGAGTT ATTAAATGCT 
ATTAAACCAG GACTCGTAAA AAAGATCAAC AGACTTCCTA CTCCCATTGC TGGATTGGAC 
AATATAACAT TATTCCTTAG AGGCTGCAAA GAGCTCGGGC TGAAAGAATC TCAGTTGTTT 
GACCCAGGTG ACCTACAGGA CACAGCAAAC AGGACAACAG CCAGGAATTC AGATTGCAAC 
AGAAAACTAA GAAATGTACT AATCACCGTG TACTGGCTGG GGAAAGCTGC AAGCAGCTGC 
TCGTCCTATA GTGGCAACAA CCTGGACTTA AAGGAGTTTG AGGGATTGCT GGCACAAATG 
AGAAAAGAAA ATGAGGAAAT AGAGAGTCCA AAACGCAGCA TACGGGACAG TGGCTACATA 
GATTGTTGGG ACTCTGAGAG AAGTGACTCC CTTTCTCCTC CCAGACATGG TAGAGATGAC 
TCCTTCGATA GTTTAGACTC ATTTGGATCT AGATCACAAC AGACACCATC TCCAGATGTA 
GTGCTGAGGG GAAGCAGCGA CGGCAGAGGA AGCGACTCTG AGTCTGACCT ACCTCACAGA 
AGGTTACCTG ATGTGCGAAA AGATGATATG TCTGCTAGAC GGGTATCCTA CGGTGAAAAC 
AAAGCATTTG TGCCTTTCAA CCAATACCTC CCCAACAAGA GCAACCAGAC CTCCTACATC 
CCGGCACCCC TGAGGAAGAA GAAGGCTGAG CGTGAGGACT ACCGGAAAAG CTGGAGCACA 
GCTACATCCC CCCTGGGGGG AGAGCGACCC TTCAGTCCCA AACAAAGTCC AGTCATTAAA 
GAAGAACAAA ACGAGTTGCA GTGTGAAGGA GAAGGGACCA AGCAGATGAT TGCCTTAAAA 
GAACCTTGCC TTGGAGCAGA AAATAGGAGT GAGAGAGTTG GTGAAAGTGA AGGGGGTGTC 
ATGGGACAAT CATCTGCTAA TGATGACATT CCAAAAGATG CAGAGGAAGT GAGAAAGCGC 
AAGCACCTTC AGAAAGCGGG TATCAAGGTC TTGCCCGCAA CAAAGCGCTA CAGCAGTCCA 
AGTCAATTTG CTGAAGACAT TGATGGCGCT CCAGACATTG TGCTGCGAAA AGAGAACACC 
TTCCTGAAGT CATATGAAGG GATGGGCTCT GATTCCGAAG AAGAAGAAGA GTGCAAAGTA 
CCTGATATAC AGCGAGATGA CTTGGCCACA AGGCGTGCAA GGATGAACCA ACCAAAAAGC 
CCAATGCAGT TTAATCAGTA TATACAGGGG TCATACTTAA ACAAAAACAG AGCATCAAGA 
AAGCTGAACA GTTCTAAGAC TTCCACTCCA GCTTCTCCAA AAATGTCTCA AGATATCAGG 
GAGTACCAAG GCCCCCAGTT ATTAAAGCCA TCACACGTCT GCAAAGAAAA GACATTTCCA 
ATACCCTCAG ACAATAAAGA GGATGCAGAA GCAGAGCAAG AAAAAGTTCC CGACATTCAG 
AAAGACGACC TGGCGAAACG CAAAGCTAAA GCTTCAGTTT CACTGCCAAA GGAACCATCG 
GCCTTTGCAG CGGTTTCAAT TACAAAGGCC GACATGGAAA CTTGGCAGAG GCTTCGAATG 
TCTTCTGAAG ATAGTGAGCA CTTGAATGAC AGGCAGGCTG ACCAGCAAGA GCAAACATCC 
AAGATTGATG TGACTAGAGC GACAAATGAT GACTTTGGGA GGAGGAAATT GTCTCTGCAA 
GAGAAAAGGG CTCGAAACAA ACAGAAGTTT GTACGCTTTG GCCCAGTGAC TGAAATTGAT 
CAGCAGAAGT GGGATAGGCT TTGTATAGCC AAGGCTGAAT CTGGCCAGGA AAGTGAAGAG 
ATGAAGGGCA GCATTACGTC TGATGAAACT GCCAGCCACA TATCTCCCTC AGCTTCTGCT 
CAGCGCTCCC AACCCCAGGA GGATGTTGGC ACAGGGCCAG TCTTGCCACA TGAGTCAAGA 
TCTTTTACAC CAGAGAGATT GGTTTCACCT GCTCCTCCTG AAAATGAAAG TGAAATTCAG 
CTCCTAGAAA CACAGCAAGA TGACATGATG GCAAGGAGAA CAGGTACATT CCAAAAATGC 
GCCAGAACCA GCAGAGACTC TCCTGTGTCC CACCAATATG CACTCCATAG CCAACAACAC 
GAAGAGAAGT CTGACTCGCT GGAACTCGAA GAGAAACAAG GACTCTTTCT GGATGTTAAC 
CCACAACTGA GAAAATTACT GCTGTCGGAC TTGGCAAATA CTAGTTCAAA AGCAACTAGC 
CCTACTTTGG GGAGCAAGTC TCTGAAAGTC ACCAAAGAAG CTGAAGATGT AAAAAATGAC 
ATGAAAGAAG GTGCCTTGAA CACAGGAAAG GATCCAAACT CTGTTATCCC AGAGGATAAC 
ACAATTGCAC CAGACCCAGA AAAAGATGAC ATGATGACAA GAAAAATCTC AGTGAGCCAG 
AAGCCATCTT GCGTCAGCCT TGGACACTTC CTTCCTGTTC CTTTTTCTTT GCAGCGCAGA 
GAGGGGGAAA AGAAACCAAC AAGCTGCATC ATATCAAGCA CCAAAAAGTC CAAAAATGCA 
TCCAAAGCTG TTCCAGCCGA CATTTCTGAT GTTCCTTCTA AGCCCCTTGA CCAGAAAGAA 
ACAAAAGAAC ATGGTGAATT AGGAGATACG ACTTGCAAGC AAGCCTCCAA TAGTGTGGAG 
ACTCCCATGT GTACAGAGTT AGACAGCCAG CAACAGGATG GTCTTGAGAG CTCCACTCTA 
TCGATCAAAA TAAAGTCAGA TAATTGCACC CAAGCACTTG CAGTCGGCAG TTCTGACCTT 
CCTTGTAAGC TCCTCGGCCA ATGTGAAACA GAGGGACATG TTGAATTACG ATCCGATGTG 
ACTTGCGATC AAACCTCCAC TGAGATCATT AGTGATGGGA TTCCCACTGG TAAAGGGTTA 
GATACCCAGG CAGAAGACGC TCTTGAGACA TGTGACCATG AGTTATTCAG ATTCAGCCCA 
AGATCTGTTC TGTCTGATGA TGGAGAGAGC ATGAGCATGT GCGACATGAG GTACGAAGAA 
GAGGGAAGTA TGCAGCCTCA CAGCAGGGCA CACTATGAGC ATCTACAGCA TGTCAACAGC 
TTGCTCAAGG AGGAGGATGA CACATGGCAA GATGACCTAG CTAAGTGGAA GACACGGAGA 
AGAAGTGCAT CACAGGATCT GATCAAAAAA GAGGAACAGA GGAAGAAGAT GGAGAGAATG 
ATGAGCGGAG AGGATGAACT GTCGGAGCGT AGGAAAAGCA TAAAAACATA TAGAGAGATT 
GTGGAAGAAA AAGAACGTCG AGAGAAAGAA CTGCATGAAG CCTACAAAAA TGCCAAGTCA 
AGAGATGAGG CTGAACACAT ATTACAGAGC TACATTGAAA AGTTTACAAT TAGTGAAGCT 
GTTCTTGAGG GATTAGTAAT GCCAAAAATC TTAGAAAGAA GCCAGTCGGT GGAGCCAAGT 
TTATTGTCTT CATCAGAGGA CCCAAACATC CTGAAATACC TGAGGCAACA GTCTTTACCA 
TCACCAAAAT ACACTTCCAC TGTTGAAGCA ACTGTCTCCC CCAGCAGCCC ATGTGAAGCA 
AAAAGTCCAA CAGGTCTGAT ATCACCAAGC AAAAACATTA TCTCAAAGAC TGTGCCTATG 
CTAACAGCCA AGCCTTACTC CCAGCCCAAA AACACCCAGG AAGTCCTCAA GACATTTAAG 
GTAGATGGAA AGGTAAACCT CAACGGGGTA GAAGAGCAGA AAGAAGAGGA ACATGGAAAC 
CAGACATTTG CCCCATCACT CACCCGATCT CAGATGTTTG AAGGTGTAGC CAGAGTTGAT 
GTTCCTGTTG TTGAAATAAA GCAAGACGTA ACTTCCATAG AGCTTAATCT GGCCAGACCA 
AACACCCTGA ATTCCTTACA AGAACCCAAA AGTCTCTGCG AAGACAGAAA TCACATAAGC 
CAGGAGGATG AGTCAGATTC TCTGGTGCAA TTAGAAAGAG CTCACTCCGT GGAAATATTG 
TCAGAAGGTA ACACCCAATC AAGCAATGCA ACCCAGGATG TACCTGTTGT GAGCAGTATA 
GAGATTCCAC CATTGACCAT CAATGGAAAG GACACTGTGG ATGAGAGGAA AGTGGAAGCT 
GCCGAGTTGA TCATGCATTG GTCCCTGGAG CCGAATCATA AGGAAGCCAA AGAAAATTCT 
TTATCGCCTT TAAAAACTTC TGAAAATTTA GAAGAATCCT TGCAGTTTAA AGAGAATGAA 
TCATCACACA AAGTGCAGCT AAACATTACG CTTCAGAGCC GGAACGCCTG GAGACGCTCC 
CAGTTTTTTT TGCAGCCAGT GGATACAGAA TGTACAGAAA ATTCACCAGC ACTTGTACCC 
AGTTTGAATT CTCCTAACCG ATTCTGCACA TGGGACCCTG AAGAAGAACG CAAACGGCAA 
GAAAAATGGC AGCAGGAGCA GGAAAGGCTT CTCCAGGAGA GGTACCAGAG AGAGCAGGAA 
AAACTTAAAG AAGAATGGGA AAAAGCTCAG AAAGAAGTAG AAGAGGAGGA AAGGAAATAT 
TATGAGGAGG AAAGAAAGAT AATTGAAGAC ACTGTGGTCC CACTGACTTT GTCTCCAAAT 
TCTAATGCAC AGTTTAATAT CTCTGAAAGA AATGGGAACT TCATTCCAGC ACTTACAGAA 
CATGTCAAGA GAGATCAAGA AAATAACAAT GAAAAGCTCT TGACAGAGTC CAGCAGAACG 
ATAAAGAATG AGACACATAT GCACAGCAAT GTAACACAGC AACTGCTTTT GCATACACAG 
GAGGGGTCAA TTCTTGGTGC TAAAGAGAAA CAAATAAAGG GAAAAACAGA CAAGTGGGAA 
GCAGAACAGT CCATCTGTAT TATGGACCGA GAGGAGCTGA GCAACCATGT GCAGTCTAAT 
GAATGTAATA CACCCACTAG GTTACCCTTG CAGGCAGGAT GTAAACAAGT GACGTCATCA 
GAGTCAAGTC TGCCTGGGGA TCTTGGATCA CCAAAAAATC CTATCACTGA AAAAGATGCT 
ATTCAGAATA TGTCGTCCAT AATACCCACA CGGGGAACTG ATTTCAAGGA AGATACAATG 
AATTCTTCAC AGTCCTCTAC ATCACAGTGC CAGTCACCAA ACAGGTCTGT TAGTGGGAAA 
AAACTGTGCT CCACATGTGG ACTTCCTCTA GGCAAGGGAG CTGCCATGAT AATTGAAACC 
CTCAGTCTTT ATTTCCACAT CCAGTGCTTC AAGTGTGGAC TGTGTAAGGG ACAACTTGGG 
GATGCGATCA CCGGCACAGA CGTCAGAATT CGAAATGGCC TACTGAACTG CAATGACTGT 
TACATGAAGT CTAGAAGTAA GGCGGCTGGC CACCCCACCC CTTTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

limch1 ( XM_031895386.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_031751246.1 Xenopus tropicalis LIM and calponin homology domains 1 (limch1), transcript variant X9, mRNA.

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CloneID	OXa49702
Clone ID Related Accession (Same CDS sequence)	XM_031895386.1
Accession Version	XM_031895386.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5298bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1576512000000
Organism	Xenopus tropicalis(tropical clawed frog)
Product	LIM and calponin homology domains-containing protein 1 isoform X8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGAAAAG AAAATGAGGA AATAGAGAGT CCAAAACGCA GCATACGGGA CAGTGGCTAC 
ATAGATTGTT GGGACTCTGA GAGAAGTGAC TCCCTTTCTC CTCCCAGACA TGGTAGAGAT 
GACTCCTTCG ATAGTTTAGA CTCATTTGGA TCTAGATCAC AACAGACACC ATCTCCAGAT 
GTAGTGCTGA GGGGAAGCAG CGACGGCAGA GGAAGCGACT CTGAGTCTGA CCTACCTCAC 
AGAAGGTTAC CTGATGTGCG AAAAGATGAT ATGTCTGCTA GACGGGTATC CTACGGTGAA 
AACAAAGCAT TTGTGCCTTT CAACCAATAC CTCCCCAACA AGAGCAACCA GACCTCCTAC 
ATCCCGGCAC CCCTGAGGAA GAAGAAGGCT GAGCGTGAGG ACTACCGGAA AAGCTGGAGC 
ACAGCTACAT CCCCCCTGGG GGGAGAGCGA CCCTTCAGTC CCAAACAAAG TCCAGTCATT 
AAAGAAGAAC AAAACGAGTT GCAGTGTGAA GGAGAAGGGA CCAAGCAGAT GATTGCCTTA 
AAAGAACCTT GCCTTGGAGC AGAAAATAGG AGTGAGAGAG TTGGTGAAAG TGAAGGGGGT 
GTCATGGGAC AATCATCTGC TAATGATGAC ATTCCAAAAG ATGCAGAGGA AGTGAGAAAG 
CGCAAGCACC TTCAGAAAGC GGGTATCAAG GTCTTGCCCG CAACAAAGCG CTACAGCAGT 
CCAAGTCAAT TTGCTGAAGA CATTGATGGC GCTCCAGACA TTGTGCTGCG AAAAGAGAAC 
ACCTTCCTGA AGTCATATGA AGGGATGGGC TCTGATTCCG AAGAAGAAGA AGAGTGCAAA 
GTACCTGATA TACAGCGAGA TGACTTGGCC ACAAGGCGTG CAAGGATGAA CCAACCAAAA 
AGCCCAATGC AGTTTAATCA GTATATACAG GGGTCATACT TAAACAAAAA CAGAGCATCA 
AGAAAGCTGA ACAGTTCTAA GACTTCCACT CCAGCTTCTC CAAAAATGTC TCAAGATATC 
AGGGAGTACC AAGGCCCCCA GTTATTAAAG CCATCACACG TCTGCAAAGA AAAGACATTT 
CCAATACCCT CAGACAATAA AGAGGATGCA GAAGCAGAGC AAGAAAAAGT TCCCGACATT 
CAGAAAGACG ACCTGGCGAA ACGCAAAGCT AAAGCTTCAG TTTCACTGCC AAAGGAACCA 
TCGGCCTTTG CAGCGGTTTC AATTACAAAG GCCGACATGG AAACTTGGCA GAGGCTTCGA 
ATGTCTTCTG AAGATAGTGA GCACTTGAAT GACAGGCAGG CTGACCAGCA AGAGCAAACA 
TCCAAGATTG ATGTGACTAG AGCGACAAAT GATGACTTTG GGAGGAGGAA ATTGTCTCTG 
CAAGAGAAAA GGGCTCGAAA CAAACAGAAG TTTGTACGCT TTGGCCCAGT GACTGAAATT 
GATCAGCAGA AGTGGGATAG GCTTTGTATA GCCAAGGCTG AATCTGGCCA GGAAAGTGAA 
GAGATGAAGG GCAGCATTAC GTCTGATGAA ACTGCCAGCC ACATATCTCC CTCAGCTTCT 
GCTCAGCGCT CCCAACCCCA GGAGGATGTT GGCACAGGGC CAGTCTTGCC ACATGAGTCA 
AGATCTTTTA CACCAGAGAG ATTGGTTTCA CCTGCTCCTC CTGAAAATGA AAGTGAAATT 
CAGCTCCTAG AAACACAGCA AGATGACATG ATGGCAAGGA GAACAGGTAC ATTCCAAAAA 
TGCGCCAGAA CCAGCAGAGA CTCTCCTGTG TCCCACCAAT ATGCACTCCA TAGCCAACAA 
CACGAAGAGA AGTCTGACTC GCTGGAACTC GAAGAGAAAC AAGGACTCTT TCTGGATGTT 
AACCCACAAC TGAGAAAATT ACTGCTGTCG GACTTGGCAA ATACTAGTTC AAAAGCAACT 
AGCCCTACTT TGGGGAGCAA GTCTCTGAAA GTCACCAAAG AAGCTGAAGA TGTAAAAAAT 
GACATGAAAG AAGGTGCCTT GAACACAGGA AAGGATCCAA ACTCTGTTAT CCCAGAGGAT 
AACACAATTG CACCAGACCC AGAAAAAGAT GACATGATGA CAAGAAAAAT CTCAGTGAGC 
CAGAAGCCAT CTTGCGTCAG CCTTGGACAC TTCCTTCCTG TTCCTTTTTC TTTGCAGCGC 
AGAGAGGGGG AAAAGAAACC AACAAGCTGC ATCATATCAA GCACCAAAAA GTCCAAAAAT 
GCATCCAAAG CTGTTCCAGC CGACATTTCT GATGTTCCTT CTAAGCCCCT TGACCAGAAA 
GAAACAAAAG AACATGGTGA ATTAGGAGAT ACGACTTGCA AGCAAGCCTC CAATAGTGTG 
GAGACTCCCA TGTGTACAGA GTTAGACAGC CAGCAACAGG ATGGTCTTGA GAGGAGAATG 
ATACTGTCTA CTGACTTGGC CAATACTGAG TTAAAAATAA CTCCTCCTAC TTTGGCCAAC 
AACTCTCCAA CCATAACAAA AGTACAAGAG CCAGATGCAA CCATCTCAGA GGACAATACA 
ACTGCACCAA ACCCTGAAAA AGATGATATG ATGACCAGAA GAATCTCTGT AACCCAGAAA 
CCATCGTGTG TAGGTCTTGG CCACTTCCTT CCAGTTCCAT TTTCTTTGCA GCAAAAAGTT 
GAGGAAAAGA AATCAACGAG CTCCACTCTA TCGATCAAAA TAAAGTCAGA TAATTGCACC 
CAAGCACTTG CAGTCGGCAG TTCTGACCTT CCTTGTAAGC TCCTCGGCCA ATGTGAAACA 
GAGGGACATG TTGAATTACG ATCCGATGTG ACTTGCGATC AAACCTCCAC TGAGATCATT 
AGTGATGGGA TTCCCACTGG TAAAGGGTTA GATACCCAGG CAGAAGACGC TCTTGAGACA 
TGTGACCATG AGTTATTCAG ATTCAGCCCA AGATCTGTTC TGTCTGATGA TGGAGAGAGC 
ATGAGCATGT GCGACATGAG GTACGAAGAA GAGGGAAGTA TGCAGCCTCA CAGCAGGGCA 
CACTATGAGC ATCTACAGCA TGTCAACAGC TTGCTCAAGG AGGAGGATGA CACATGGCAA 
GATGACCTAG CTAAGTGGAA GACACGGAGA AGAAGTGCAT CACAGGATCT GATCAAAAAA 
GAGGAACAGA GGAAGAAGAT GGAGAGAATG ATGAGCGGAG AGGATGAACT GTCGGAGCGT 
AGGAAAAGCA TAAAAACATA TAGAGAGATT GTGGAAGAAA AAGAACGTCG AGAGAAAGAA 
CTGCATGAAG CCTACAAAAA TGCCAAGTCA AGAGATGAGG CTGAACACAT ATTACAGAGC 
TACATTGAAA AGTTTACAAT TAGTGAAGCT GTTCTTGAGG GATTAGTAAT GCCAAAAATC 
TTAGAAAGAA GCCAGTCGGT GGAGCCAAGT TTATTGTCTT CATCAGAGGA CCCAAACATC 
CTGAAATACC TGAGGCAACA GTCTTTACCA TCACCAAAAT ACACTTCCAC TGTTGAAGCA 
ACTGTCTCCC CCAGCAGCCC ATGTGAAGCA AAAAGTCCAA CAGGTCTGAT ATCACCAAGC 
AAAAACATTA TCTCAAAGAC TGTGCCTATG CTAACAGCCA AGCCTTACTC CCAGCCCAAA 
AACACCCAGG AAGTCCTCAA GACATTTAAG GTAGATGGAA AGGTAAACCT CAACGGGGTA 
GAAGAGCAGA AAGAAGAGGA ACATGGAAAC CAGACATTTG CCCCATCACT CACCCGATCT 
CAGATGTTTG AAGGTGTAGC CAGAGTTGAT GTTCCTGTTG TTGAAATAAA GCAAGACGTA 
ACTTCCATAG AGCTTAATCT GGCCAGACCA AACACCCTGA ATTCCTTACA AGAACCCAAA 
AGTCTCTGCG AAGACAGAAA TCACATAAGC CAGGAGGATG AGTCAGATTC TCTGGTGCAA 
TTAGAAAGAG CTCACTCCGT GGAAATATTG TCAGAAGGTA ACACCCAATC AAGCAATGCA 
ACCCAGGATG TACCTGTTGT GAGCAGTATA GAGATTCCAC CATTGACCAT CAATGGAAAG 
GACACTGTGG ATGAGAGGAA AGTGGAAGCT GCCGAGTTGA TCATGCATTG GTCCCTGGAG 
CCGAATCATA AGGAAGCCAA AGAAAATTCT TTATCGCCTT TAAAAACTTC TGAAAATTTA 
GAAGAATCCT TGCAGTTTAA AGAGAATGAA TCATCACACA AAGTGCAGCT AAACATTACG 
CTTCAGAGCC GGAACGCCTG GAGACGCTCC CAGTTTTTTT TGCAGCCAGT GGATACAGAA 
TGTACAGAAA ATTCACCAGC ACTTGTACCC AGTTTGAATT CTCCTAACCG ATTCTGCACA 
TGGGACCCTG AAGAAGAACG CAAACGGCAA GAAAAATGGC AGCAGGAGCA GGAAAGGCTT 
CTCCAGGAGA GGTACCAGAG AGAGCAGGAA AAACTTAAAG AAGAATGGGA AAAAGCTCAG 
AAAGAAGTAG AAGAGGAGGA AAGGAAATAT TATGAGGAGG AAAGAAAGAT AATTGAAGAC 
ACTGTGGTCC CACTGACTTT GTCTCCAAAT TCTAATGCAC AGTTTAATAT CTCTGAAAGA 
AATGGGAACT TCATTCCAGC ACTTACAGAA CATGTCAAGA GAGATCAAGA AAATAACAAT 
GAAAAGCTCT TGACAGAGTC CAGCAGAACG ATAAAGAATG AGACACATAT GCACAGCAAT 
GTAACACAGC AACTGCTTTT GCATACACAG GAGGGGTCAA TTCTTGGTGC TAAAGAGAAA 
CAAATAAAGG GAAAAACAGA CAAGTGGGAA GCAGAACAGT CCATCTGTAT TATGGACCGA 
GAGGAGCTGA GCAACCATGT GCAGTCTAAT GAATGTAATA CACCCACTAG GTTACCCTTG 
CAGGCAGGAT GTAAACAAGT GACGTCATCA GAGTCAAGTC TGCCTGGGGA TCTTGGATCA 
CCAAAAAATC CTATCACTGA AAAAGATGCT ATTCAGAATA TGTCGTCCAT AATACCCACA 
CGGGGAACTG ATTTCAAGGA AGATACAATG AATTCTTCAC AGTCCTCTAC ATCACAGTGC 
CAGTCACCAA ACAGGTCTGT TAGTGGGAAA AAACTGTGCT CCACATGTGG ACTTCCTCTA 
GGCAAGGGAG CTGCCATGAT AATTGAAACC CTCAGTCTTT ATTTCCACAT CCAGTGCTTC 
AAGTGTGGAC TGTGTAAGGG ACAACTTGGG GATGCGATCA CCGGCACAGA CGTCAGAATT 
CGAAATGGCC TACTGAACTG CAATGACTGT TACATGAAGT CTAGAAGTAA GGCGGCTGGC 
CACCCCACCC CTTTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031751246.1
CDS	250..5547
Translation	MRKENEEIESPKRSIRDSGYIDCWDSERSDSLSPPRHGRDDSFDSLDSFGSRSQQTPSPDVVLRGSSDGRGSDSESDLPHRRLPDVRKDDMSARRVSYGENKAFVPFNQYLPNKSNQTSYIPAPLRKKKAEREDYRKSWSTATSPLGGERPFSPKQSPVIKEEQNELQCEGEGTKQMIALKEPCLGAENRSERVGESEGGVMGQSSANDDIPKDAEEVRKRKHLQKAGIKVLPATKRYSSPSQFAEDIDGAPDIVLRKENTFLKSYEGMGSDSEEEEECKVPDIQRDDLATRRARMNQPKSPMQFNQYIQGSYLNKNRASRKLNSSKTSTPASPKMSQDIREYQGPQLLKPSHVCKEKTFPIPSDNKEDAEAEQEKVPDIQKDDLAKRKAKASVSLPKEPSAFAAVSITKADMETWQRLRMSSEDSEHLNDRQADQQEQTSKIDVTRATNDDFGRRKLSLQEKRARNKQKFVRFGPVTEIDQQKWDRLCIAKAESGQESEEMKGSITSDETASHISPSASAQRSQPQEDVGTGPVLPHESRSFTPERLVSPAPPENESEIQLLETQQDDMMARRTGTFQKCARTSRDSPVSHQYALHSQQHEEKSDSLELEEKQGLFLDVNPQLRKLLLSDLANTSSKATSPTLGSKSLKVTKEAEDVKNDMKEGALNTGKDPNSVIPEDNTIAPDPEKDDMMTRKISVSQKPSCVSLGHFLPVPFSLQRREGEKKPTSCIISSTKKSKNASKAVPADISDVPSKPLDQKETKEHGELGDTTCKQASNSVETPMCTELDSQQQDGLERRMILSTDLANTELKITPPTLANNSPTITKVQEPDATISEDNTTAPNPEKDDMMTRRISVTQKPSCVGLGHFLPVPFSLQQKVEEKKSTSSTLSIKIKSDNCTQALAVGSSDLPCKLLGQCETEGHVELRSDVTCDQTSTEIISDGIPTGKGLDTQAEDALETCDHELFRFSPRSVLSDDGESMSMCDMRYEEEGSMQPHSRAHYEHLQHVNSLLKEEDDTWQDDLAKWKTRRRSASQDLIKKEEQRKKMERMMSGEDELSERRKSIKTYREIVEEKERREKELHEAYKNAKSRDEAEHILQSYIEKFTISEAVLEGLVMPKILERSQSVEPSLLSSSEDPNILKYLRQQSLPSPKYTSTVEATVSPSSPCEAKSPTGLISPSKNIISKTVPMLTAKPYSQPKNTQEVLKTFKVDGKVNLNGVEEQKEEEHGNQTFAPSLTRSQMFEGVARVDVPVVEIKQDVTSIELNLARPNTLNSLQEPKSLCEDRNHISQEDESDSLVQLERAHSVEILSEGNTQSSNATQDVPVVSSIEIPPLTINGKDTVDERKVEAAELIMHWSLEPNHKEAKENSLSPLKTSENLEESLQFKENESSHKVQLNITLQSRNAWRRSQFFLQPVDTECTENSPALVPSLNSPNRFCTWDPEEERKRQEKWQQEQERLLQERYQREQEKLKEEWEKAQKEVEEEERKYYEEERKIIEDTVVPLTLSPNSNAQFNISERNGNFIPALTEHVKRDQENNNEKLLTESSRTIKNETHMHSNVTQQLLLHTQEGSILGAKEKQIKGKTDKWEAEQSICIMDREELSNHVQSNECNTPTRLPLQAGCKQVTSSESSLPGDLGSPKNPITEKDAIQNMSSIIPTRGTDFKEDTMNSSQSSTSQCQSPNRSVSGKKLCSTCGLPLGKGAAMIIETLSLYFHIQCFKCGLCKGQLGDAITGTDVRIRNGLLNCNDCYMKSRSKAAGHPTPL

Target ORF information:

RefSeq Version	XM_031895386.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis LIM and calponin homology domains 1 (limch1), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031895386.1

ORF Insert Sequence:

ATGAGAAAAG AAAATGAGGA AATAGAGAGT CCAAAACGCA GCATACGGGA CAGTGGCTAC 
ATAGATTGTT GGGACTCTGA GAGAAGTGAC TCCCTTTCTC CTCCCAGACA TGGTAGAGAT 
GACTCCTTCG ATAGTTTAGA CTCATTTGGA TCTAGATCAC AACAGACACC ATCTCCAGAT 
GTAGTGCTGA GGGGAAGCAG CGACGGCAGA GGAAGCGACT CTGAGTCTGA CCTACCTCAC 
AGAAGGTTAC CTGATGTGCG AAAAGATGAT ATGTCTGCTA GACGGGTATC CTACGGTGAA 
AACAAAGCAT TTGTGCCTTT CAACCAATAC CTCCCCAACA AGAGCAACCA GACCTCCTAC 
ATCCCGGCAC CCCTGAGGAA GAAGAAGGCT GAGCGTGAGG ACTACCGGAA AAGCTGGAGC 
ACAGCTACAT CCCCCCTGGG GGGAGAGCGA CCCTTCAGTC CCAAACAAAG TCCAGTCATT 
AAAGAAGAAC AAAACGAGTT GCAGTGTGAA GGAGAAGGGA CCAAGCAGAT GATTGCCTTA 
AAAGAACCTT GCCTTGGAGC AGAAAATAGG AGTGAGAGAG TTGGTGAAAG TGAAGGGGGT 
GTCATGGGAC AATCATCTGC TAATGATGAC ATTCCAAAAG ATGCAGAGGA AGTGAGAAAG 
CGCAAGCACC TTCAGAAAGC GGGTATCAAG GTCTTGCCCG CAACAAAGCG CTACAGCAGT 
CCAAGTCAAT TTGCTGAAGA CATTGATGGC GCTCCAGACA TTGTGCTGCG AAAAGAGAAC 
ACCTTCCTGA AGTCATATGA AGGGATGGGC TCTGATTCCG AAGAAGAAGA AGAGTGCAAA 
GTACCTGATA TACAGCGAGA TGACTTGGCC ACAAGGCGTG CAAGGATGAA CCAACCAAAA 
AGCCCAATGC AGTTTAATCA GTATATACAG GGGTCATACT TAAACAAAAA CAGAGCATCA 
AGAAAGCTGA ACAGTTCTAA GACTTCCACT CCAGCTTCTC CAAAAATGTC TCAAGATATC 
AGGGAGTACC AAGGCCCCCA GTTATTAAAG CCATCACACG TCTGCAAAGA AAAGACATTT 
CCAATACCCT CAGACAATAA AGAGGATGCA GAAGCAGAGC AAGAAAAAGT TCCCGACATT 
CAGAAAGACG ACCTGGCGAA ACGCAAAGCT AAAGCTTCAG TTTCACTGCC AAAGGAACCA 
TCGGCCTTTG CAGCGGTTTC AATTACAAAG GCCGACATGG AAACTTGGCA GAGGCTTCGA 
ATGTCTTCTG AAGATAGTGA GCACTTGAAT GACAGGCAGG CTGACCAGCA AGAGCAAACA 
TCCAAGATTG ATGTGACTAG AGCGACAAAT GATGACTTTG GGAGGAGGAA ATTGTCTCTG 
CAAGAGAAAA GGGCTCGAAA CAAACAGAAG TTTGTACGCT TTGGCCCAGT GACTGAAATT 
GATCAGCAGA AGTGGGATAG GCTTTGTATA GCCAAGGCTG AATCTGGCCA GGAAAGTGAA 
GAGATGAAGG GCAGCATTAC GTCTGATGAA ACTGCCAGCC ACATATCTCC CTCAGCTTCT 
GCTCAGCGCT CCCAACCCCA GGAGGATGTT GGCACAGGGC CAGTCTTGCC ACATGAGTCA 
AGATCTTTTA CACCAGAGAG ATTGGTTTCA CCTGCTCCTC CTGAAAATGA AAGTGAAATT 
CAGCTCCTAG AAACACAGCA AGATGACATG ATGGCAAGGA GAACAGGTAC ATTCCAAAAA 
TGCGCCAGAA CCAGCAGAGA CTCTCCTGTG TCCCACCAAT ATGCACTCCA TAGCCAACAA 
CACGAAGAGA AGTCTGACTC GCTGGAACTC GAAGAGAAAC AAGGACTCTT TCTGGATGTT 
AACCCACAAC TGAGAAAATT ACTGCTGTCG GACTTGGCAA ATACTAGTTC AAAAGCAACT 
AGCCCTACTT TGGGGAGCAA GTCTCTGAAA GTCACCAAAG AAGCTGAAGA TGTAAAAAAT 
GACATGAAAG AAGGTGCCTT GAACACAGGA AAGGATCCAA ACTCTGTTAT CCCAGAGGAT 
AACACAATTG CACCAGACCC AGAAAAAGAT GACATGATGA CAAGAAAAAT CTCAGTGAGC 
CAGAAGCCAT CTTGCGTCAG CCTTGGACAC TTCCTTCCTG TTCCTTTTTC TTTGCAGCGC 
AGAGAGGGGG AAAAGAAACC AACAAGCTGC ATCATATCAA GCACCAAAAA GTCCAAAAAT 
GCATCCAAAG CTGTTCCAGC CGACATTTCT GATGTTCCTT CTAAGCCCCT TGACCAGAAA 
GAAACAAAAG AACATGGTGA ATTAGGAGAT ACGACTTGCA AGCAAGCCTC CAATAGTGTG 
GAGACTCCCA TGTGTACAGA GTTAGACAGC CAGCAACAGG ATGGTCTTGA GAGGAGAATG 
ATACTGTCTA CTGACTTGGC CAATACTGAG TTAAAAATAA CTCCTCCTAC TTTGGCCAAC 
AACTCTCCAA CCATAACAAA AGTACAAGAG CCAGATGCAA CCATCTCAGA GGACAATACA 
ACTGCACCAA ACCCTGAAAA AGATGATATG ATGACCAGAA GAATCTCTGT AACCCAGAAA 
CCATCGTGTG TAGGTCTTGG CCACTTCCTT CCAGTTCCAT TTTCTTTGCA GCAAAAAGTT 
GAGGAAAAGA AATCAACGAG CTCCACTCTA TCGATCAAAA TAAAGTCAGA TAATTGCACC 
CAAGCACTTG CAGTCGGCAG TTCTGACCTT CCTTGTAAGC TCCTCGGCCA ATGTGAAACA 
GAGGGACATG TTGAATTACG ATCCGATGTG ACTTGCGATC AAACCTCCAC TGAGATCATT 
AGTGATGGGA TTCCCACTGG TAAAGGGTTA GATACCCAGG CAGAAGACGC TCTTGAGACA 
TGTGACCATG AGTTATTCAG ATTCAGCCCA AGATCTGTTC TGTCTGATGA TGGAGAGAGC 
ATGAGCATGT GCGACATGAG GTACGAAGAA GAGGGAAGTA TGCAGCCTCA CAGCAGGGCA 
CACTATGAGC ATCTACAGCA TGTCAACAGC TTGCTCAAGG AGGAGGATGA CACATGGCAA 
GATGACCTAG CTAAGTGGAA GACACGGAGA AGAAGTGCAT CACAGGATCT GATCAAAAAA 
GAGGAACAGA GGAAGAAGAT GGAGAGAATG ATGAGCGGAG AGGATGAACT GTCGGAGCGT 
AGGAAAAGCA TAAAAACATA TAGAGAGATT GTGGAAGAAA AAGAACGTCG AGAGAAAGAA 
CTGCATGAAG CCTACAAAAA TGCCAAGTCA AGAGATGAGG CTGAACACAT ATTACAGAGC 
TACATTGAAA AGTTTACAAT TAGTGAAGCT GTTCTTGAGG GATTAGTAAT GCCAAAAATC 
TTAGAAAGAA GCCAGTCGGT GGAGCCAAGT TTATTGTCTT CATCAGAGGA CCCAAACATC 
CTGAAATACC TGAGGCAACA GTCTTTACCA TCACCAAAAT ACACTTCCAC TGTTGAAGCA 
ACTGTCTCCC CCAGCAGCCC ATGTGAAGCA AAAAGTCCAA CAGGTCTGAT ATCACCAAGC 
AAAAACATTA TCTCAAAGAC TGTGCCTATG CTAACAGCCA AGCCTTACTC CCAGCCCAAA 
AACACCCAGG AAGTCCTCAA GACATTTAAG GTAGATGGAA AGGTAAACCT CAACGGGGTA 
GAAGAGCAGA AAGAAGAGGA ACATGGAAAC CAGACATTTG CCCCATCACT CACCCGATCT 
CAGATGTTTG AAGGTGTAGC CAGAGTTGAT GTTCCTGTTG TTGAAATAAA GCAAGACGTA 
ACTTCCATAG AGCTTAATCT GGCCAGACCA AACACCCTGA ATTCCTTACA AGAACCCAAA 
AGTCTCTGCG AAGACAGAAA TCACATAAGC CAGGAGGATG AGTCAGATTC TCTGGTGCAA 
TTAGAAAGAG CTCACTCCGT GGAAATATTG TCAGAAGGTA ACACCCAATC AAGCAATGCA 
ACCCAGGATG TACCTGTTGT GAGCAGTATA GAGATTCCAC CATTGACCAT CAATGGAAAG 
GACACTGTGG ATGAGAGGAA AGTGGAAGCT GCCGAGTTGA TCATGCATTG GTCCCTGGAG 
CCGAATCATA AGGAAGCCAA AGAAAATTCT TTATCGCCTT TAAAAACTTC TGAAAATTTA 
GAAGAATCCT TGCAGTTTAA AGAGAATGAA TCATCACACA AAGTGCAGCT AAACATTACG 
CTTCAGAGCC GGAACGCCTG GAGACGCTCC CAGTTTTTTT TGCAGCCAGT GGATACAGAA 
TGTACAGAAA ATTCACCAGC ACTTGTACCC AGTTTGAATT CTCCTAACCG ATTCTGCACA 
TGGGACCCTG AAGAAGAACG CAAACGGCAA GAAAAATGGC AGCAGGAGCA GGAAAGGCTT 
CTCCAGGAGA GGTACCAGAG AGAGCAGGAA AAACTTAAAG AAGAATGGGA AAAAGCTCAG 
AAAGAAGTAG AAGAGGAGGA AAGGAAATAT TATGAGGAGG AAAGAAAGAT AATTGAAGAC 
ACTGTGGTCC CACTGACTTT GTCTCCAAAT TCTAATGCAC AGTTTAATAT CTCTGAAAGA 
AATGGGAACT TCATTCCAGC ACTTACAGAA CATGTCAAGA GAGATCAAGA AAATAACAAT 
GAAAAGCTCT TGACAGAGTC CAGCAGAACG ATAAAGAATG AGACACATAT GCACAGCAAT 
GTAACACAGC AACTGCTTTT GCATACACAG GAGGGGTCAA TTCTTGGTGC TAAAGAGAAA 
CAAATAAAGG GAAAAACAGA CAAGTGGGAA GCAGAACAGT CCATCTGTAT TATGGACCGA 
GAGGAGCTGA GCAACCATGT GCAGTCTAAT GAATGTAATA CACCCACTAG GTTACCCTTG 
CAGGCAGGAT GTAAACAAGT GACGTCATCA GAGTCAAGTC TGCCTGGGGA TCTTGGATCA 
CCAAAAAATC CTATCACTGA AAAAGATGCT ATTCAGAATA TGTCGTCCAT AATACCCACA 
CGGGGAACTG ATTTCAAGGA AGATACAATG AATTCTTCAC AGTCCTCTAC ATCACAGTGC 
CAGTCACCAA ACAGGTCTGT TAGTGGGAAA AAACTGTGCT CCACATGTGG ACTTCCTCTA 
GGCAAGGGAG CTGCCATGAT AATTGAAACC CTCAGTCTTT ATTTCCACAT CCAGTGCTTC 
AAGTGTGGAC TGTGTAAGGG ACAACTTGGG GATGCGATCA CCGGCACAGA CGTCAGAATT 
CGAAATGGCC TACTGAACTG CAATGACTGT TACATGAAGT CTAGAAGTAA GGCGGCTGGC 
CACCCCACCC CTTTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gene Symbol	limch1
Entrez Gene ID	100127553
Full Name	LIM and calponin homology domains 1
General protein information	Preferred Names LIM and calponin homology domains 1 Names LIM and calponin homology domains-containing protein 1
Gene Type	protein-coding
Organism	Xenopus tropicalis(tropical clawed frog)

Canis lupus familiaris (dog)	LIMCH1	XP_005628192.1
Gallus gallus (chicken)	LIMCH1	XP_004936138.1
Xenopus tropicalis (tropical clawed frog)	limch1	XP_004911276.1
Pan troglodytes (chimpanzee)	LIMCH1	XP_517164.3
Macaca mulatta (Rhesus monkey)	LOC707416	XP_002808455.1
Bos taurus (cattle)	LIMCH1	NP_001178450.1
Mus musculus (house mouse)	Limch1	NP_001001980.2
Homo sapiens (human)	LIMCH1	NP_055803.2
Rattus norvegicus (Norway rat)	Limch1	NP_001178607.1
Danio rerio (zebrafish)	limch1b	NP_001108201.1

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

limch1 cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

Gene

Clones

gRNAs

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