timeless cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

Gene
Clones
gRNAs

Summary

Gene Symbol	timeless
Entrez Gene ID	100126683
Full Name	timeless circadian regulator
Synonyms	XTimeless1,htim,tim,tim1,timeless-a,timeless-b
General protein information	Preferred Names timeless circadian regulator Names protein timeless homolog timeless circadian clock timeless homolog
Gene Type	protein-coding
Organism	Xenopus tropicalis(tropical clawed frog)

mRNA and Protein(s)

mRNA	Protein	Name
XM_002934529.4	XP_002934575.1	protein timeless homolog
XM_002934529.5	XP_002934575.1	protein timeless homolog
XM_031897167.1	XP_031753027.1	protein timeless homolog

Pathways from BioSystems

Homologies

Homo sapiens (human)	TIMELESS	NP_003911.2
Canis lupus familiaris (dog)	TIMELESS	XP_538233.3
Pan troglodytes (chimpanzee)	TIMELESS	XP_509535.2
Macaca mulatta (Rhesus monkey)	LOC712835	XP_002808027.1
Danio rerio (zebrafish)	timeless	NP_001265529.1
Xenopus tropicalis (tropical clawed frog)	timeless	XP_002934575.1
Bos taurus (cattle)	TIMELESS	NP_001095541.2
Mus musculus (house mouse)	Timeless	NP_035719.1
Rattus norvegicus (Norway rat)	Timeless	NP_112630.1
Drosophila melanogaster (fruit fly)	timeout	NP_524341.3
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP010787	XP_001689129.1

Gene Ontology

The following timeless gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the timeless cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OXa06023	XM_031897167.1 Latest version!	Xenopus tropicalis timeless circadian regulator (timeless), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OXa06023	XM_002934529.5 Latest version!	Xenopus tropicalis timeless circadian regulator (timeless), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
View Old Accession Versions >>
OXa06023	XM_002934529.4	Xenopus tropicalis timeless circadian clock (timeless), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

timeless ( XM_002934529.4 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_002934575.1 Xenopus tropicalis timeless circadian clock (timeless), mRNA.

Price & Availability↑

CloneID	OXa06023
Clone ID Related Accession (Same CDS sequence)	XM_002934529.4 , XM_002934529.5 , XM_031897167.1
Accession Version	XM_002934529.4	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3864bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1473177600000
Organism	Xenopus tropicalis(tropical clawed frog)
Product	protein timeless homolog
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030678.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_002934529.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACTTGT ACATGATGAA CTGTGAATTG TTAGCAACGT GCAGTGCCTT GGGGTACCTG 
GAGAGCGATA CCTACCACAA AGAGCCTGAC TGTCTTGAGA GTGTAAAAGA TTTAATTCGT 
TACTTGCGCC ATGAGGATGA TACAAGAGAT ATCAGACAGC AGCTTGGGGC TGCCCAGATA 
CTGCAAAATG ATCTTATTCC CCTGGTTATC CAGCATCACA ATGATAAACA GCTGTTTGAT 
GCTGTAATCA GGTTGATGGT AAATGTAACG CAGCCGGCAC TTTTGTGCTT TGGGAAAGTA 
CCTCAGGATC CTACATTTAG ACACCATTTT CTGCAGGTTG TTTCCTATTT ACAAGCTTAC 
AAGGAGGCAT TTGCAAATGA AAGACTATTT GGAGTCTTAA GTGAAAAATT ATATGATTTA 
CTGCAGATGG AATGGGAGCA ACGAGAGGAG GAACATAATT TACTTATAGA AAGGATTCTT 
CTGCTAGTGC GTAATATTCT CCATGTGCCC CCTGACCCAG AGGAGGAGAA AAGTGTAGAT 
GACGATGCTA GTATTCATGA CCGAGTGCTT TGGGCAATCC ACATGAGTGG AATGGATGAT 
CTAATTAAGT TTTTGGCTAG CTCAGAGACA GAGCAACAAT GGAGCATGCA TGTGCTGGAG 
ACTGTATCTC TTATGTTTAG AGACCAGATA GCAGAGCATT TAGCTGCAAC AGGCCAGGCA 
CGCTCAACTG CAGAGCGAGA TACTGATATT AAAAACTTGG AGATCCTGAG ACAGAGGGAA 
TCAGCAGAAA AGAAGAACAG GGCTCTTCAG CGTGGGACTC GGCATTCTCG GTTTGGTGGT 
TCTTACATTG TTCAGGGACT GAAATCTATT GGAGACAAAG ATGTAATCTA TCACAAAGGC 
ATTCACAATT TCAAGAACTA CACTCATGAC CTTGGAAAGG AAGTGCGGAG AGTCCCAAAG 
AGAAAGCAGC AGGCCAAGGA AACAGAGGCA CGACGCCGCT CTGCACTCAA TGTTCGATTA 
TTTCTAAAGG AGTTTTGCAT TGACTTTCTT GAAAATTGCT ACAATCGACT CATGTACATT 
GTAAAAGATA ATCTGATTCG TGAGAAGGCA CAGCAGCATG ACGAAACCTA CTACTTGTGG 
TCCATGGCTT TTTTTATGGG GTTTAATCGC CTCTTCCGCT TCCAACCTGA GCTTGTGTCT 
GAGACAGTTA GCATCAGGAC CTTCCACTTC ATAGAGCAGA ACCTCACAAA TTATTATGAG 
ATGATGCTGA CTGACAAGAA GGAGGCCACA TCATGGGCCC GGCGAATGCA CCTTGCTCTA 
AAAGCGTATC AAGAGCTTCT AAGCACTGTT CATGAGATGG ACCATTCTAA GGAAGAGGCT 
GTGAGGGAAA GCAGCAAAAT CATTAAAAAT AACATATTCT ATTTAATGGA GTACCGAGAG 
CTCTTTCTGG CGCTATTCAG GAAATTTGAT GAGACTAAGC AGCCACGTTC CTTCTTAAAG 
GATCTGGTGG AGACAACACA TTTGTTTCTT AAAATGTTAG AAAAATTTTG CAAAGGAAAA 
CAGACTGTGG TCGTACAGAA ACGCCGTGTG AAACGAAAGA AGAAGAAATC CTCTGTAAGT 
GGTGCTAAGG AGGTTCAGCC TAAGAGCACA GAGGAGCTGG CGGCAATATG GACAAATGTT 
TCAGAGCAGC TCACGACCTG CATAGTGAAT TCAGAAAATC TACCTGACAG TGTTGTGCCT 
TTTGATGCTG CCTCTGAGAT ACCAGTAGAA GAGCAGAGGG CAGAAGCCTT AGTACGAATC 
CAAGATGGTC TCTTCTCTGG CTGTGCAGCA GAGGCCCTTG TTCTGCTGCG TTCTGCTAGG 
GAGGTCTGGC CAGAGGGGGA TGTTTTTGGT GCTGCAGAGA TAGAACCTGA AGAAGAGACT 
GAGCTACTGA AACAAATTCT CTTTGCAGAG CTTCCCAGAT CAACACAGGC TGAAAGTGAG 
GTTCTGGGAG AGGAAGAAGA GGAAGATGAG ATTGAGGAAG AAGAACTTAC CTCAGTTCAA 
GTATCTGAGA GGGAATTTAA CTTTCTAGAT TACATGAAAC GGTTTGCTAA CTCCAATGTA 
GTGAAAACCT ATGTCCTGCT GCTGAAGAAT TACCAACAGA ACAGTGCTCA CACCAATCAC 
TGTGTAGCGA AGATGTTGCA CCGTATTGCA TATGACCTCA AAATGGAGGC ACTTCTCTTT 
CAGCTTTCAG TGTTTTGTAT TTTCAACAAA ATTCTGGGTG ATCCAGCAGC AAGTGCCTAT 
AAGGAGTTGG TGGCCTTTTC TAAATACATC TTAAATAAGT TCTTTTCCCT GGCGGCAACA 
AACAACAAGG CCTATGTGGA GCTTCTTTTC TGGAAAAACA CCAATGTGAT TAGAGAAATG 
ACAGAGGGCT ATAGAAAACT TGGAGAAGAA GAGAGTGAAG CGAACCGCAA GCGCATAAAG 
TGGACTCCAG AGGATGAGGA TGAGCTGAGG AGACTTTATT TGCAGTTCAA AGAGATAGAA 
GACGCAGATG TGGTGGATAA TATCGTCGCC AACTTAAAAC CTGGAACCCG TACTCGGAAG 
CAAGTCATTA ACCATTTGGT GCAAATGGGG CTAGCTGACA GTGTCAAGGA CTTCAAACAA 
GAAACAAAAG GAACTCGCAT TGTGCTATGG ACAGAGGATC AAGAGCTGGA ACTTCAGCGC 
CTTTATGAAG ACTTTAAAGA CTCAGATGAC ATATTGGGAA ATATAATGAA GAATGTGACT 
GCAAAACGCT CCAAGGCTAG AATAATTGAG AAGCTTATCA GCATGGGTCT AGTATGCGAT 
CGCAAAGAAC TTTATAAGAA AAGAAGGAAG AAGGGGAAAT CATCTGGCAT GTCTGAAGAA 
GAGTTTCTCA ATGATCTGGG GTTTGATGGA AAACACAGTG TGGATGATGT GGAAGAAGAG 
GAGGAGGAGG AAGAAGAAGA AGCAAGTGAA GAAGAAGAAG AGTGTGAAGA CCTTAACTAT 
AAACAAATTG CAAAAGAGCA AACAGGTGGC AGAATGGATC CATTTACCGA TAAGAATATA 
AAGCATTTGG TGCACAAGTT ACAGCAGGAA GGTTTATCTG GTCCTCTTTT GTGGCTTCAA 
AACTGTTTAA ACCGCACTGC CGAAGACAGA GAAGAGGATG GCTCTTCTCA GGCAATTCCC 
CTTGTGCCTT TAACGGAAGA CAATGAGGAT GCTATGGAAA ATAAAACATT CAAGAAACTG 
CTGAAGAAGT TGGGCATCCG TGCGCCTGCA AATGAGCAGG AATCTTTCTG GCGCATCCCA 
GGTAAATTAA GTCCGGAACA GCTGCGCAAG GCAGCCTCCT GTCTTGTTCC CTTGGCTGCA 
GCAGTTGCCT GTGAGGTTCT TGCAAATGCT GAAGAACAAG AGGTAAATGT TGAGCAGAGG 
GCAGAAGCAC TTCGTGCACT TTTACTTGCA CGGAAAAAGA AATCATCAGA AGCTCAGCAA 
GACCTTCAAG AAGAAAATCA GAAATCCCCA GATAAGTCAT TACAATCCCA AAGCAAAACC 
AGAAGACCAA AGACAGCAAA GAAGAGAAGT AAAATGGTTG ACAGTGATGA AGAGAATTCT 
GTAGACAATA CTAATGAGAA CAAGGAGTCA GAGCAGCTGC CAATGGATGC CATAACAGAG 
AGAGAAACCA AACCGAATAG ACCAAAGAAA TCAAAGAAAA GAAGTCGTAT AGTGGACAGT 
GATGATGACT CTGTGACAGA AGAGCATGGC AAGGAGATGT TAGAGGTAGA CCAGAAAGGA 
TCGTTTTCTG AATCGGACAC TGAGGAAAAT GTTCAACCTA CCAAGAAAAA GCGTCTTGTC 
ATTGAGGATG AAGATGAAGA TTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_002934575.1
CDS	183..4046
Translation	MDLYMMNCELLATCSALGYLESDTYHKEPDCLESVKDLIRYLRHEDDTRDIRQQLGAAQILQNDLIPLVIQHHNDKQLFDAVIRLMVNVTQPALLCFGKVPQDPTFRHHFLQVVSYLQAYKEAFANERLFGVLSEKLYDLLQMEWEQREEEHNLLIERILLLVRNILHVPPDPEEEKSVDDDASIHDRVLWAIHMSGMDDLIKFLASSETEQQWSMHVLETVSLMFRDQIAEHLAATGQARSTAERDTDIKNLEILRQRESAEKKNRALQRGTRHSRFGGSYIVQGLKSIGDKDVIYHKGIHNFKNYTHDLGKEVRRVPKRKQQAKETEARRRSALNVRLFLKEFCIDFLENCYNRLMYIVKDNLIREKAQQHDETYYLWSMAFFMGFNRLFRFQPELVSETVSIRTFHFIEQNLTNYYEMMLTDKKEATSWARRMHLALKAYQELLSTVHEMDHSKEEAVRESSKIIKNNIFYLMEYRELFLALFRKFDETKQPRSFLKDLVETTHLFLKMLEKFCKGKQTVVVQKRRVKRKKKKSSVSGAKEVQPKSTEELAAIWTNVSEQLTTCIVNSENLPDSVVPFDAASEIPVEEQRAEALVRIQDGLFSGCAAEALVLLRSAREVWPEGDVFGAAEIEPEEETELLKQILFAELPRSTQAESEVLGEEEEEDEIEEEELTSVQVSEREFNFLDYMKRFANSNVVKTYVLLLKNYQQNSAHTNHCVAKMLHRIAYDLKMEALLFQLSVFCIFNKILGDPAASAYKELVAFSKYILNKFFSLAATNNKAYVELLFWKNTNVIREMTEGYRKLGEEESEANRKRIKWTPEDEDELRRLYLQFKEIEDADVVDNIVANLKPGTRTRKQVINHLVQMGLADSVKDFKQETKGTRIVLWTEDQELELQRLYEDFKDSDDILGNIMKNVTAKRSKARIIEKLISMGLVCDRKELYKKRRKKGKSSGMSEEEFLNDLGFDGKHSVDDVEEEEEEEEEEASEEEEECEDLNYKQIAKEQTGGRMDPFTDKNIKHLVHKLQQEGLSGPLLWLQNCLNRTAEDREEDGSSQAIPLVPLTEDNEDAMENKTFKKLLKKLGIRAPANEQESFWRIPGKLSPEQLRKAASCLVPLAAAVACEVLANAEEQEVNVEQRAEALRALLLARKKKSSEAQQDLQEENQKSPDKSLQSQSKTRRPKTAKKRSKMVDSDEENSVDNTNENKESEQLPMDAITERETKPNRPKKSKKRSRIVDSDDDSVTEEHGKEMLEVDQKGSFSESDTEENVQPTKKKRLVIEDEDED

Target ORF information:

RefSeq Version	XM_002934529.4
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis timeless circadian clock (timeless), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_002934529.4

ORF Insert Sequence:

ATGGACTTGT ACATGATGAA CTGTGAATTG TTAGCAACGT GCAGTGCCTT GGGGTACCTG 
GAGAGCGATA CCTACCACAA AGAGCCTGAC TGTCTTGAGA GTGTAAAAGA TTTAATTCGT 
TACTTGCGCC ATGAGGATGA TACAAGAGAT ATCAGACAGC AGCTTGGGGC TGCCCAGATA 
CTGCAAAATG ATCTTATTCC CCTGGTTATC CAGCATCACA ATGATAAACA GCTGTTTGAT 
GCTGTAATCA GGTTGATGGT AAATGTAACG CAGCCGGCAC TTTTGTGCTT TGGGAAAGTA 
CCTCAGGATC CTACATTTAG ACACCATTTT CTGCAGGTTG TTTCCTATTT ACAAGCTTAC 
AAGGAGGCAT TTGCAAATGA AAGACTATTT GGAGTCTTAA GTGAAAAATT ATATGATTTA 
CTGCAGATGG AATGGGAGCA ACGAGAGGAG GAACATAATT TACTTATAGA AAGGATTCTT 
CTGCTAGTGC GTAATATTCT CCATGTGCCC CCTGACCCAG AGGAGGAGAA AAGTGTAGAT 
GACGATGCTA GTATTCATGA CCGAGTGCTT TGGGCAATCC ACATGAGTGG AATGGATGAT 
CTAATTAAGT TTTTGGCTAG CTCAGAGACA GAGCAACAAT GGAGCATGCA TGTGCTGGAG 
ACTGTATCTC TTATGTTTAG AGACCAGATA GCAGAGCATT TAGCTGCAAC AGGCCAGGCA 
CGCTCAACTG CAGAGCGAGA TACTGATATT AAAAACTTGG AGATCCTGAG ACAGAGGGAA 
TCAGCAGAAA AGAAGAACAG GGCTCTTCAG CGTGGGACTC GGCATTCTCG GTTTGGTGGT 
TCTTACATTG TTCAGGGACT GAAATCTATT GGAGACAAAG ATGTAATCTA TCACAAAGGC 
ATTCACAATT TCAAGAACTA CACTCATGAC CTTGGAAAGG AAGTGCGGAG AGTCCCAAAG 
AGAAAGCAGC AGGCCAAGGA AACAGAGGCA CGACGCCGCT CTGCACTCAA TGTTCGATTA 
TTTCTAAAGG AGTTTTGCAT TGACTTTCTT GAAAATTGCT ACAATCGACT CATGTACATT 
GTAAAAGATA ATCTGATTCG TGAGAAGGCA CAGCAGCATG ACGAAACCTA CTACTTGTGG 
TCCATGGCTT TTTTTATGGG GTTTAATCGC CTCTTCCGCT TCCAACCTGA GCTTGTGTCT 
GAGACAGTTA GCATCAGGAC CTTCCACTTC ATAGAGCAGA ACCTCACAAA TTATTATGAG 
ATGATGCTGA CTGACAAGAA GGAGGCCACA TCATGGGCCC GGCGAATGCA CCTTGCTCTA 
AAAGCGTATC AAGAGCTTCT AAGCACTGTT CATGAGATGG ACCATTCTAA GGAAGAGGCT 
GTGAGGGAAA GCAGCAAAAT CATTAAAAAT AACATATTCT ATTTAATGGA GTACCGAGAG 
CTCTTTCTGG CGCTATTCAG GAAATTTGAT GAGACTAAGC AGCCACGTTC CTTCTTAAAG 
GATCTGGTGG AGACAACACA TTTGTTTCTT AAAATGTTAG AAAAATTTTG CAAAGGAAAA 
CAGACTGTGG TCGTACAGAA ACGCCGTGTG AAACGAAAGA AGAAGAAATC CTCTGTAAGT 
GGTGCTAAGG AGGTTCAGCC TAAGAGCACA GAGGAGCTGG CGGCAATATG GACAAATGTT 
TCAGAGCAGC TCACGACCTG CATAGTGAAT TCAGAAAATC TACCTGACAG TGTTGTGCCT 
TTTGATGCTG CCTCTGAGAT ACCAGTAGAA GAGCAGAGGG CAGAAGCCTT AGTACGAATC 
CAAGATGGTC TCTTCTCTGG CTGTGCAGCA GAGGCCCTTG TTCTGCTGCG TTCTGCTAGG 
GAGGTCTGGC CAGAGGGGGA TGTTTTTGGT GCTGCAGAGA TAGAACCTGA AGAAGAGACT 
GAGCTACTGA AACAAATTCT CTTTGCAGAG CTTCCCAGAT CAACACAGGC TGAAAGTGAG 
GTTCTGGGAG AGGAAGAAGA GGAAGATGAG ATTGAGGAAG AAGAACTTAC CTCAGTTCAA 
GTATCTGAGA GGGAATTTAA CTTTCTAGAT TACATGAAAC GGTTTGCTAA CTCCAATGTA 
GTGAAAACCT ATGTCCTGCT GCTGAAGAAT TACCAACAGA ACAGTGCTCA CACCAATCAC 
TGTGTAGCGA AGATGTTGCA CCGTATTGCA TATGACCTCA AAATGGAGGC ACTTCTCTTT 
CAGCTTTCAG TGTTTTGTAT TTTCAACAAA ATTCTGGGTG ATCCAGCAGC AAGTGCCTAT 
AAGGAGTTGG TGGCCTTTTC TAAATACATC TTAAATAAGT TCTTTTCCCT GGCGGCAACA 
AACAACAAGG CCTATGTGGA GCTTCTTTTC TGGAAAAACA CCAATGTGAT TAGAGAAATG 
ACAGAGGGCT ATAGAAAACT TGGAGAAGAA GAGAGTGAAG CGAACCGCAA GCGCATAAAG 
TGGACTCCAG AGGATGAGGA TGAGCTGAGG AGACTTTATT TGCAGTTCAA AGAGATAGAA 
GACGCAGATG TGGTGGATAA TATCGTCGCC AACTTAAAAC CTGGAACCCG TACTCGGAAG 
CAAGTCATTA ACCATTTGGT GCAAATGGGG CTAGCTGACA GTGTCAAGGA CTTCAAACAA 
GAAACAAAAG GAACTCGCAT TGTGCTATGG ACAGAGGATC AAGAGCTGGA ACTTCAGCGC 
CTTTATGAAG ACTTTAAAGA CTCAGATGAC ATATTGGGAA ATATAATGAA GAATGTGACT 
GCAAAACGCT CCAAGGCTAG AATAATTGAG AAGCTTATCA GCATGGGTCT AGTATGCGAT 
CGCAAAGAAC TTTATAAGAA AAGAAGGAAG AAGGGGAAAT CATCTGGCAT GTCTGAAGAA 
GAGTTTCTCA ATGATCTGGG GTTTGATGGA AAACACAGTG TGGATGATGT GGAAGAAGAG 
GAGGAGGAGG AAGAAGAAGA AGCAAGTGAA GAAGAAGAAG AGTGTGAAGA CCTTAACTAT 
AAACAAATTG CAAAAGAGCA AACAGGTGGC AGAATGGATC CATTTACCGA TAAGAATATA 
AAGCATTTGG TGCACAAGTT ACAGCAGGAA GGTTTATCTG GTCCTCTTTT GTGGCTTCAA 
AACTGTTTAA ACCGCACTGC CGAAGACAGA GAAGAGGATG GCTCTTCTCA GGCAATTCCC 
CTTGTGCCTT TAACGGAAGA CAATGAGGAT GCTATGGAAA ATAAAACATT CAAGAAACTG 
CTGAAGAAGT TGGGCATCCG TGCGCCTGCA AATGAGCAGG AATCTTTCTG GCGCATCCCA 
GGTAAATTAA GTCCGGAACA GCTGCGCAAG GCAGCCTCCT GTCTTGTTCC CTTGGCTGCA 
GCAGTTGCCT GTGAGGTTCT TGCAAATGCT GAAGAACAAG AGGTAAATGT TGAGCAGAGG 
GCAGAAGCAC TTCGTGCACT TTTACTTGCA CGGAAAAAGA AATCATCAGA AGCTCAGCAA 
GACCTTCAAG AAGAAAATCA GAAATCCCCA GATAAGTCAT TACAATCCCA AAGCAAAACC 
AGAAGACCAA AGACAGCAAA GAAGAGAAGT AAAATGGTTG ACAGTGATGA AGAGAATTCT 
GTAGACAATA CTAATGAGAA CAAGGAGTCA GAGCAGCTGC CAATGGATGC CATAACAGAG 
AGAGAAACCA AACCGAATAG ACCAAAGAAA TCAAAGAAAA GAAGTCGTAT AGTGGACAGT 
GATGATGACT CTGTGACAGA AGAGCATGGC AAGGAGATGT TAGAGGTAGA CCAGAAAGGA 
TCGTTTTCTG AATCGGACAC TGAGGAAAAT GTTCAACCTA CCAAGAAAAA GCGTCTTGTC 
ATTGAGGATG AAGATGAAGA TTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

timeless ( XM_002934529.5 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_002934575.1 Xenopus tropicalis timeless circadian regulator (timeless), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OXa06023
Clone ID Related Accession (Same CDS sequence)	XM_002934529.4 , XM_002934529.5 , XM_031897167.1
Accession Version	XM_002934529.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3864bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1576512000000
Organism	Xenopus tropicalis(tropical clawed frog)
Product	protein timeless homolog
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030678.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_002934529.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACTTGT ACATGATGAA CTGTGAATTG TTAGCAACGT GCAGTGCCTT GGGGTACCTG 
GAGAGCGATA CCTACCACAA AGAGCCTGAC TGTCTTGAGA GTGTAAAAGA TTTAATTCGT 
TACTTGCGCC ATGAGGATGA TACAAGAGAT ATCAGACAGC AGCTTGGGGC TGCCCAGATA 
CTGCAAAATG ATCTTATTCC CCTGGTTATC CAGCATCACA ATGATAAACA GCTGTTTGAT 
GCTGTAATCA GGTTGATGGT AAATGTAACG CAGCCGGCAC TTTTGTGCTT TGGGAAAGTA 
CCTCAGGATC CTACATTTAG ACACCATTTT CTGCAGGTTG TTTCCTATTT ACAAGCTTAC 
AAGGAGGCAT TTGCAAATGA AAGACTATTT GGAGTCTTAA GTGAAAAATT ATATGATTTA 
CTGCAGATGG AATGGGAGCA ACGAGAGGAG GAACATAATT TACTTATAGA AAGGATTCTT 
CTGCTAGTGC GTAATATTCT CCATGTGCCC CCTGACCCAG AGGAGGAGAA AAGTGTAGAT 
GACGATGCTA GTATTCATGA CCGAGTGCTT TGGGCAATCC ACATGAGTGG AATGGATGAT 
CTAATTAAGT TTTTGGCTAG CTCAGAGACA GAGCAACAAT GGAGCATGCA TGTGCTGGAG 
ACTGTATCTC TTATGTTTAG AGACCAGATA GCAGAGCATT TAGCTGCAAC AGGCCAGGCA 
CGCTCAACTG CAGAGCGAGA TACTGATATT AAAAACTTGG AGATCCTGAG ACAGAGGGAA 
TCAGCAGAAA AGAAGAACAG GGCTCTTCAG CGTGGGACTC GGCATTCTCG GTTTGGTGGT 
TCTTACATTG TTCAGGGACT GAAATCTATT GGAGACAAAG ATGTAATCTA TCACAAAGGC 
ATTCACAATT TCAAGAACTA CACTCATGAC CTTGGAAAGG AAGTGCGGAG AGTCCCAAAG 
AGAAAGCAGC AGGCCAAGGA AACAGAGGCA CGACGCCGCT CTGCACTCAA TGTTCGATTA 
TTTCTAAAGG AGTTTTGCAT TGACTTTCTT GAAAATTGCT ACAATCGACT CATGTACATT 
GTAAAAGATA ATCTGATTCG TGAGAAGGCA CAGCAGCATG ACGAAACCTA CTACTTGTGG 
TCCATGGCTT TTTTTATGGG GTTTAATCGC CTCTTCCGCT TCCAACCTGA GCTTGTGTCT 
GAGACAGTTA GCATCAGGAC CTTCCACTTC ATAGAGCAGA ACCTCACAAA TTATTATGAG 
ATGATGCTGA CTGACAAGAA GGAGGCCACA TCATGGGCCC GGCGAATGCA CCTTGCTCTA 
AAAGCGTATC AAGAGCTTCT AAGCACTGTT CATGAGATGG ACCATTCTAA GGAAGAGGCT 
GTGAGGGAAA GCAGCAAAAT CATTAAAAAT AACATATTCT ATTTAATGGA GTACCGAGAG 
CTCTTTCTGG CGCTATTCAG GAAATTTGAT GAGACTAAGC AGCCACGTTC CTTCTTAAAG 
GATCTGGTGG AGACAACACA TTTGTTTCTT AAAATGTTAG AAAAATTTTG CAAAGGAAAA 
CAGACTGTGG TCGTACAGAA ACGCCGTGTG AAACGAAAGA AGAAGAAATC CTCTGTAAGT 
GGTGCTAAGG AGGTTCAGCC TAAGAGCACA GAGGAGCTGG CGGCAATATG GACAAATGTT 
TCAGAGCAGC TCACGACCTG CATAGTGAAT TCAGAAAATC TACCTGACAG TGTTGTGCCT 
TTTGATGCTG CCTCTGAGAT ACCAGTAGAA GAGCAGAGGG CAGAAGCCTT AGTACGAATC 
CAAGATGGTC TCTTCTCTGG CTGTGCAGCA GAGGCCCTTG TTCTGCTGCG TTCTGCTAGG 
GAGGTCTGGC CAGAGGGGGA TGTTTTTGGT GCTGCAGAGA TAGAACCTGA AGAAGAGACT 
GAGCTACTGA AACAAATTCT CTTTGCAGAG CTTCCCAGAT CAACACAGGC TGAAAGTGAG 
GTTCTGGGAG AGGAAGAAGA GGAAGATGAG ATTGAGGAAG AAGAACTTAC CTCAGTTCAA 
GTATCTGAGA GGGAATTTAA CTTTCTAGAT TACATGAAAC GGTTTGCTAA CTCCAATGTA 
GTGAAAACCT ATGTCCTGCT GCTGAAGAAT TACCAACAGA ACAGTGCTCA CACCAATCAC 
TGTGTAGCGA AGATGTTGCA CCGTATTGCA TATGACCTCA AAATGGAGGC ACTTCTCTTT 
CAGCTTTCAG TGTTTTGTAT TTTCAACAAA ATTCTGGGTG ATCCAGCAGC AAGTGCCTAT 
AAGGAGTTGG TGGCCTTTTC TAAATACATC TTAAATAAGT TCTTTTCCCT GGCGGCAACA 
AACAACAAGG CCTATGTGGA GCTTCTTTTC TGGAAAAACA CCAATGTGAT TAGAGAAATG 
ACAGAGGGCT ATAGAAAACT TGGAGAAGAA GAGAGTGAAG CGAACCGCAA GCGCATAAAG 
TGGACTCCAG AGGATGAGGA TGAGCTGAGG AGACTTTATT TGCAGTTCAA AGAGATAGAA 
GACGCAGATG TGGTGGATAA TATCGTCGCC AACTTAAAAC CTGGAACCCG TACTCGGAAG 
CAAGTCATTA ACCATTTGGT GCAAATGGGG CTAGCTGACA GTGTCAAGGA CTTCAAACAA 
GAAACAAAAG GAACTCGCAT TGTGCTATGG ACAGAGGATC AAGAGCTGGA ACTTCAGCGC 
CTTTATGAAG ACTTTAAAGA CTCAGATGAC ATATTGGGAA ATATAATGAA GAATGTGACT 
GCAAAACGCT CCAAGGCTAG AATAATTGAG AAGCTTATCA GCATGGGTCT AGTATGCGAT 
CGCAAAGAAC TTTATAAGAA AAGAAGGAAG AAGGGGAAAT CATCTGGCAT GTCTGAAGAA 
GAGTTTCTCA ATGATCTGGG GTTTGATGGA AAACACAGTG TGGATGATGT GGAAGAAGAG 
GAGGAGGAGG AAGAAGAAGA AGCAAGTGAA GAAGAAGAAG AGTGTGAAGA CCTTAACTAT 
AAACAAATTG CAAAAGAGCA AACAGGTGGC AGAATGGATC CATTTACCGA TAAGAATATA 
AAGCATTTGG TGCACAAGTT ACAGCAGGAA GGTTTATCTG GTCCTCTTTT GTGGCTTCAA 
AACTGTTTAA ACCGCACTGC CGAAGACAGA GAAGAGGATG GCTCTTCTCA GGCAATTCCC 
CTTGTGCCTT TAACGGAAGA CAATGAGGAT GCTATGGAAA ATAAAACATT CAAGAAACTG 
CTGAAGAAGT TGGGCATCCG TGCGCCTGCA AATGAGCAGG AATCTTTCTG GCGCATCCCA 
GGTAAATTAA GTCCGGAACA GCTGCGCAAG GCAGCCTCCT GTCTTGTTCC CTTGGCTGCA 
GCAGTTGCCT GTGAGGTTCT TGCAAATGCT GAAGAACAAG AGGTAAATGT TGAGCAGAGG 
GCAGAAGCAC TTCGTGCACT TTTACTTGCA CGGAAAAAGA AATCATCAGA AGCTCAGCAA 
GACCTTCAAG AAGAAAATCA GAAATCCCCA GATAAGTCAT TACAATCCCA AAGCAAAACC 
AGAAGACCAA AGACAGCAAA GAAGAGAAGT AAAATGGTTG ACAGTGATGA AGAGAATTCT 
GTAGACAATA CTAATGAGAA CAAGGAGTCA GAGCAGCTGC CAATGGATGC CATAACAGAG 
AGAGAAACCA AACCGAATAG ACCAAAGAAA TCAAAGAAAA GAAGTCGTAT AGTGGACAGT 
GATGATGACT CTGTGACAGA AGAGCATGGC AAGGAGATGT TAGAGGTAGA CCAGAAAGGA 
TCGTTTTCTG AATCGGACAC TGAGGAAAAT GTTCAACCTA CCAAGAAAAA GCGTCTTGTC 
ATTGAGGATG AAGATGAAGA TTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_002934575.1
CDS	178..4041
Translation	MDLYMMNCELLATCSALGYLESDTYHKEPDCLESVKDLIRYLRHEDDTRDIRQQLGAAQILQNDLIPLVIQHHNDKQLFDAVIRLMVNVTQPALLCFGKVPQDPTFRHHFLQVVSYLQAYKEAFANERLFGVLSEKLYDLLQMEWEQREEEHNLLIERILLLVRNILHVPPDPEEEKSVDDDASIHDRVLWAIHMSGMDDLIKFLASSETEQQWSMHVLETVSLMFRDQIAEHLAATGQARSTAERDTDIKNLEILRQRESAEKKNRALQRGTRHSRFGGSYIVQGLKSIGDKDVIYHKGIHNFKNYTHDLGKEVRRVPKRKQQAKETEARRRSALNVRLFLKEFCIDFLENCYNRLMYIVKDNLIREKAQQHDETYYLWSMAFFMGFNRLFRFQPELVSETVSIRTFHFIEQNLTNYYEMMLTDKKEATSWARRMHLALKAYQELLSTVHEMDHSKEEAVRESSKIIKNNIFYLMEYRELFLALFRKFDETKQPRSFLKDLVETTHLFLKMLEKFCKGKQTVVVQKRRVKRKKKKSSVSGAKEVQPKSTEELAAIWTNVSEQLTTCIVNSENLPDSVVPFDAASEIPVEEQRAEALVRIQDGLFSGCAAEALVLLRSAREVWPEGDVFGAAEIEPEEETELLKQILFAELPRSTQAESEVLGEEEEEDEIEEEELTSVQVSEREFNFLDYMKRFANSNVVKTYVLLLKNYQQNSAHTNHCVAKMLHRIAYDLKMEALLFQLSVFCIFNKILGDPAASAYKELVAFSKYILNKFFSLAATNNKAYVELLFWKNTNVIREMTEGYRKLGEEESEANRKRIKWTPEDEDELRRLYLQFKEIEDADVVDNIVANLKPGTRTRKQVINHLVQMGLADSVKDFKQETKGTRIVLWTEDQELELQRLYEDFKDSDDILGNIMKNVTAKRSKARIIEKLISMGLVCDRKELYKKRRKKGKSSGMSEEEFLNDLGFDGKHSVDDVEEEEEEEEEEASEEEEECEDLNYKQIAKEQTGGRMDPFTDKNIKHLVHKLQQEGLSGPLLWLQNCLNRTAEDREEDGSSQAIPLVPLTEDNEDAMENKTFKKLLKKLGIRAPANEQESFWRIPGKLSPEQLRKAASCLVPLAAAVACEVLANAEEQEVNVEQRAEALRALLLARKKKSSEAQQDLQEENQKSPDKSLQSQSKTRRPKTAKKRSKMVDSDEENSVDNTNENKESEQLPMDAITERETKPNRPKKSKKRSRIVDSDDDSVTEEHGKEMLEVDQKGSFSESDTEENVQPTKKKRLVIEDEDED

Target ORF information:

RefSeq Version	XM_002934529.5
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis timeless circadian regulator (timeless), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_002934529.5

ORF Insert Sequence:

ATGGACTTGT ACATGATGAA CTGTGAATTG TTAGCAACGT GCAGTGCCTT GGGGTACCTG 
GAGAGCGATA CCTACCACAA AGAGCCTGAC TGTCTTGAGA GTGTAAAAGA TTTAATTCGT 
TACTTGCGCC ATGAGGATGA TACAAGAGAT ATCAGACAGC AGCTTGGGGC TGCCCAGATA 
CTGCAAAATG ATCTTATTCC CCTGGTTATC CAGCATCACA ATGATAAACA GCTGTTTGAT 
GCTGTAATCA GGTTGATGGT AAATGTAACG CAGCCGGCAC TTTTGTGCTT TGGGAAAGTA 
CCTCAGGATC CTACATTTAG ACACCATTTT CTGCAGGTTG TTTCCTATTT ACAAGCTTAC 
AAGGAGGCAT TTGCAAATGA AAGACTATTT GGAGTCTTAA GTGAAAAATT ATATGATTTA 
CTGCAGATGG AATGGGAGCA ACGAGAGGAG GAACATAATT TACTTATAGA AAGGATTCTT 
CTGCTAGTGC GTAATATTCT CCATGTGCCC CCTGACCCAG AGGAGGAGAA AAGTGTAGAT 
GACGATGCTA GTATTCATGA CCGAGTGCTT TGGGCAATCC ACATGAGTGG AATGGATGAT 
CTAATTAAGT TTTTGGCTAG CTCAGAGACA GAGCAACAAT GGAGCATGCA TGTGCTGGAG 
ACTGTATCTC TTATGTTTAG AGACCAGATA GCAGAGCATT TAGCTGCAAC AGGCCAGGCA 
CGCTCAACTG CAGAGCGAGA TACTGATATT AAAAACTTGG AGATCCTGAG ACAGAGGGAA 
TCAGCAGAAA AGAAGAACAG GGCTCTTCAG CGTGGGACTC GGCATTCTCG GTTTGGTGGT 
TCTTACATTG TTCAGGGACT GAAATCTATT GGAGACAAAG ATGTAATCTA TCACAAAGGC 
ATTCACAATT TCAAGAACTA CACTCATGAC CTTGGAAAGG AAGTGCGGAG AGTCCCAAAG 
AGAAAGCAGC AGGCCAAGGA AACAGAGGCA CGACGCCGCT CTGCACTCAA TGTTCGATTA 
TTTCTAAAGG AGTTTTGCAT TGACTTTCTT GAAAATTGCT ACAATCGACT CATGTACATT 
GTAAAAGATA ATCTGATTCG TGAGAAGGCA CAGCAGCATG ACGAAACCTA CTACTTGTGG 
TCCATGGCTT TTTTTATGGG GTTTAATCGC CTCTTCCGCT TCCAACCTGA GCTTGTGTCT 
GAGACAGTTA GCATCAGGAC CTTCCACTTC ATAGAGCAGA ACCTCACAAA TTATTATGAG 
ATGATGCTGA CTGACAAGAA GGAGGCCACA TCATGGGCCC GGCGAATGCA CCTTGCTCTA 
AAAGCGTATC AAGAGCTTCT AAGCACTGTT CATGAGATGG ACCATTCTAA GGAAGAGGCT 
GTGAGGGAAA GCAGCAAAAT CATTAAAAAT AACATATTCT ATTTAATGGA GTACCGAGAG 
CTCTTTCTGG CGCTATTCAG GAAATTTGAT GAGACTAAGC AGCCACGTTC CTTCTTAAAG 
GATCTGGTGG AGACAACACA TTTGTTTCTT AAAATGTTAG AAAAATTTTG CAAAGGAAAA 
CAGACTGTGG TCGTACAGAA ACGCCGTGTG AAACGAAAGA AGAAGAAATC CTCTGTAAGT 
GGTGCTAAGG AGGTTCAGCC TAAGAGCACA GAGGAGCTGG CGGCAATATG GACAAATGTT 
TCAGAGCAGC TCACGACCTG CATAGTGAAT TCAGAAAATC TACCTGACAG TGTTGTGCCT 
TTTGATGCTG CCTCTGAGAT ACCAGTAGAA GAGCAGAGGG CAGAAGCCTT AGTACGAATC 
CAAGATGGTC TCTTCTCTGG CTGTGCAGCA GAGGCCCTTG TTCTGCTGCG TTCTGCTAGG 
GAGGTCTGGC CAGAGGGGGA TGTTTTTGGT GCTGCAGAGA TAGAACCTGA AGAAGAGACT 
GAGCTACTGA AACAAATTCT CTTTGCAGAG CTTCCCAGAT CAACACAGGC TGAAAGTGAG 
GTTCTGGGAG AGGAAGAAGA GGAAGATGAG ATTGAGGAAG AAGAACTTAC CTCAGTTCAA 
GTATCTGAGA GGGAATTTAA CTTTCTAGAT TACATGAAAC GGTTTGCTAA CTCCAATGTA 
GTGAAAACCT ATGTCCTGCT GCTGAAGAAT TACCAACAGA ACAGTGCTCA CACCAATCAC 
TGTGTAGCGA AGATGTTGCA CCGTATTGCA TATGACCTCA AAATGGAGGC ACTTCTCTTT 
CAGCTTTCAG TGTTTTGTAT TTTCAACAAA ATTCTGGGTG ATCCAGCAGC AAGTGCCTAT 
AAGGAGTTGG TGGCCTTTTC TAAATACATC TTAAATAAGT TCTTTTCCCT GGCGGCAACA 
AACAACAAGG CCTATGTGGA GCTTCTTTTC TGGAAAAACA CCAATGTGAT TAGAGAAATG 
ACAGAGGGCT ATAGAAAACT TGGAGAAGAA GAGAGTGAAG CGAACCGCAA GCGCATAAAG 
TGGACTCCAG AGGATGAGGA TGAGCTGAGG AGACTTTATT TGCAGTTCAA AGAGATAGAA 
GACGCAGATG TGGTGGATAA TATCGTCGCC AACTTAAAAC CTGGAACCCG TACTCGGAAG 
CAAGTCATTA ACCATTTGGT GCAAATGGGG CTAGCTGACA GTGTCAAGGA CTTCAAACAA 
GAAACAAAAG GAACTCGCAT TGTGCTATGG ACAGAGGATC AAGAGCTGGA ACTTCAGCGC 
CTTTATGAAG ACTTTAAAGA CTCAGATGAC ATATTGGGAA ATATAATGAA GAATGTGACT 
GCAAAACGCT CCAAGGCTAG AATAATTGAG AAGCTTATCA GCATGGGTCT AGTATGCGAT 
CGCAAAGAAC TTTATAAGAA AAGAAGGAAG AAGGGGAAAT CATCTGGCAT GTCTGAAGAA 
GAGTTTCTCA ATGATCTGGG GTTTGATGGA AAACACAGTG TGGATGATGT GGAAGAAGAG 
GAGGAGGAGG AAGAAGAAGA AGCAAGTGAA GAAGAAGAAG AGTGTGAAGA CCTTAACTAT 
AAACAAATTG CAAAAGAGCA AACAGGTGGC AGAATGGATC CATTTACCGA TAAGAATATA 
AAGCATTTGG TGCACAAGTT ACAGCAGGAA GGTTTATCTG GTCCTCTTTT GTGGCTTCAA 
AACTGTTTAA ACCGCACTGC CGAAGACAGA GAAGAGGATG GCTCTTCTCA GGCAATTCCC 
CTTGTGCCTT TAACGGAAGA CAATGAGGAT GCTATGGAAA ATAAAACATT CAAGAAACTG 
CTGAAGAAGT TGGGCATCCG TGCGCCTGCA AATGAGCAGG AATCTTTCTG GCGCATCCCA 
GGTAAATTAA GTCCGGAACA GCTGCGCAAG GCAGCCTCCT GTCTTGTTCC CTTGGCTGCA 
GCAGTTGCCT GTGAGGTTCT TGCAAATGCT GAAGAACAAG AGGTAAATGT TGAGCAGAGG 
GCAGAAGCAC TTCGTGCACT TTTACTTGCA CGGAAAAAGA AATCATCAGA AGCTCAGCAA 
GACCTTCAAG AAGAAAATCA GAAATCCCCA GATAAGTCAT TACAATCCCA AAGCAAAACC 
AGAAGACCAA AGACAGCAAA GAAGAGAAGT AAAATGGTTG ACAGTGATGA AGAGAATTCT 
GTAGACAATA CTAATGAGAA CAAGGAGTCA GAGCAGCTGC CAATGGATGC CATAACAGAG 
AGAGAAACCA AACCGAATAG ACCAAAGAAA TCAAAGAAAA GAAGTCGTAT AGTGGACAGT 
GATGATGACT CTGTGACAGA AGAGCATGGC AAGGAGATGT TAGAGGTAGA CCAGAAAGGA 
TCGTTTTCTG AATCGGACAC TGAGGAAAAT GTTCAACCTA CCAAGAAAAA GCGTCTTGTC 
ATTGAGGATG AAGATGAAGA TTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

timeless ( XM_031897167.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_031753027.1 Xenopus tropicalis timeless circadian regulator (timeless), transcript variant X1, mRNA.

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CloneID	OXa06023
Clone ID Related Accession (Same CDS sequence)	XM_002934529.4 , XM_002934529.5 , XM_031897167.1
Accession Version	XM_031897167.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3864bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1576512000000
Organism	Xenopus tropicalis(tropical clawed frog)
Product	protein timeless homolog
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030678.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACTTGT ACATGATGAA CTGTGAATTG TTAGCAACGT GCAGTGCCTT GGGGTACCTG 
GAGAGCGATA CCTACCACAA AGAGCCTGAC TGTCTTGAGA GTGTAAAAGA TTTAATTCGT 
TACTTGCGCC ATGAGGATGA TACAAGAGAT ATCAGACAGC AGCTTGGGGC TGCCCAGATA 
CTGCAAAATG ATCTTATTCC CCTGGTTATC CAGCATCACA ATGATAAACA GCTGTTTGAT 
GCTGTAATCA GGTTGATGGT AAATGTAACG CAGCCGGCAC TTTTGTGCTT TGGGAAAGTA 
CCTCAGGATC CTACATTTAG ACACCATTTT CTGCAGGTTG TTTCCTATTT ACAAGCTTAC 
AAGGAGGCAT TTGCAAATGA AAGACTATTT GGAGTCTTAA GTGAAAAATT ATATGATTTA 
CTGCAGATGG AATGGGAGCA ACGAGAGGAG GAACATAATT TACTTATAGA AAGGATTCTT 
CTGCTAGTGC GTAATATTCT CCATGTGCCC CCTGACCCAG AGGAGGAGAA AAGTGTAGAT 
GACGATGCTA GTATTCATGA CCGAGTGCTT TGGGCAATCC ACATGAGTGG AATGGATGAT 
CTAATTAAGT TTTTGGCTAG CTCAGAGACA GAGCAACAAT GGAGCATGCA TGTGCTGGAG 
ACTGTATCTC TTATGTTTAG AGACCAGATA GCAGAGCATT TAGCTGCAAC AGGCCAGGCA 
CGCTCAACTG CAGAGCGAGA TACTGATATT AAAAACTTGG AGATCCTGAG ACAGAGGGAA 
TCAGCAGAAA AGAAGAACAG GGCTCTTCAG CGTGGGACTC GGCATTCTCG GTTTGGTGGT 
TCTTACATTG TTCAGGGACT GAAATCTATT GGAGACAAAG ATGTAATCTA TCACAAAGGC 
ATTCACAATT TCAAGAACTA CACTCATGAC CTTGGAAAGG AAGTGCGGAG AGTCCCAAAG 
AGAAAGCAGC AGGCCAAGGA AACAGAGGCA CGACGCCGCT CTGCACTCAA TGTTCGATTA 
TTTCTAAAGG AGTTTTGCAT TGACTTTCTT GAAAATTGCT ACAATCGACT CATGTACATT 
GTAAAAGATA ATCTGATTCG TGAGAAGGCA CAGCAGCATG ACGAAACCTA CTACTTGTGG 
TCCATGGCTT TTTTTATGGG GTTTAATCGC CTCTTCCGCT TCCAACCTGA GCTTGTGTCT 
GAGACAGTTA GCATCAGGAC CTTCCACTTC ATAGAGCAGA ACCTCACAAA TTATTATGAG 
ATGATGCTGA CTGACAAGAA GGAGGCCACA TCATGGGCCC GGCGAATGCA CCTTGCTCTA 
AAAGCGTATC AAGAGCTTCT AAGCACTGTT CATGAGATGG ACCATTCTAA GGAAGAGGCT 
GTGAGGGAAA GCAGCAAAAT CATTAAAAAT AACATATTCT ATTTAATGGA GTACCGAGAG 
CTCTTTCTGG CGCTATTCAG GAAATTTGAT GAGACTAAGC AGCCACGTTC CTTCTTAAAG 
GATCTGGTGG AGACAACACA TTTGTTTCTT AAAATGTTAG AAAAATTTTG CAAAGGAAAA 
CAGACTGTGG TCGTACAGAA ACGCCGTGTG AAACGAAAGA AGAAGAAATC CTCTGTAAGT 
GGTGCTAAGG AGGTTCAGCC TAAGAGCACA GAGGAGCTGG CGGCAATATG GACAAATGTT 
TCAGAGCAGC TCACGACCTG CATAGTGAAT TCAGAAAATC TACCTGACAG TGTTGTGCCT 
TTTGATGCTG CCTCTGAGAT ACCAGTAGAA GAGCAGAGGG CAGAAGCCTT AGTACGAATC 
CAAGATGGTC TCTTCTCTGG CTGTGCAGCA GAGGCCCTTG TTCTGCTGCG TTCTGCTAGG 
GAGGTCTGGC CAGAGGGGGA TGTTTTTGGT GCTGCAGAGA TAGAACCTGA AGAAGAGACT 
GAGCTACTGA AACAAATTCT CTTTGCAGAG CTTCCCAGAT CAACACAGGC TGAAAGTGAG 
GTTCTGGGAG AGGAAGAAGA GGAAGATGAG ATTGAGGAAG AAGAACTTAC CTCAGTTCAA 
GTATCTGAGA GGGAATTTAA CTTTCTAGAT TACATGAAAC GGTTTGCTAA CTCCAATGTA 
GTGAAAACCT ATGTCCTGCT GCTGAAGAAT TACCAACAGA ACAGTGCTCA CACCAATCAC 
TGTGTAGCGA AGATGTTGCA CCGTATTGCA TATGACCTCA AAATGGAGGC ACTTCTCTTT 
CAGCTTTCAG TGTTTTGTAT TTTCAACAAA ATTCTGGGTG ATCCAGCAGC AAGTGCCTAT 
AAGGAGTTGG TGGCCTTTTC TAAATACATC TTAAATAAGT TCTTTTCCCT GGCGGCAACA 
AACAACAAGG CCTATGTGGA GCTTCTTTTC TGGAAAAACA CCAATGTGAT TAGAGAAATG 
ACAGAGGGCT ATAGAAAACT TGGAGAAGAA GAGAGTGAAG CGAACCGCAA GCGCATAAAG 
TGGACTCCAG AGGATGAGGA TGAGCTGAGG AGACTTTATT TGCAGTTCAA AGAGATAGAA 
GACGCAGATG TGGTGGATAA TATCGTCGCC AACTTAAAAC CTGGAACCCG TACTCGGAAG 
CAAGTCATTA ACCATTTGGT GCAAATGGGG CTAGCTGACA GTGTCAAGGA CTTCAAACAA 
GAAACAAAAG GAACTCGCAT TGTGCTATGG ACAGAGGATC AAGAGCTGGA ACTTCAGCGC 
CTTTATGAAG ACTTTAAAGA CTCAGATGAC ATATTGGGAA ATATAATGAA GAATGTGACT 
GCAAAACGCT CCAAGGCTAG AATAATTGAG AAGCTTATCA GCATGGGTCT AGTATGCGAT 
CGCAAAGAAC TTTATAAGAA AAGAAGGAAG AAGGGGAAAT CATCTGGCAT GTCTGAAGAA 
GAGTTTCTCA ATGATCTGGG GTTTGATGGA AAACACAGTG TGGATGATGT GGAAGAAGAG 
GAGGAGGAGG AAGAAGAAGA AGCAAGTGAA GAAGAAGAAG AGTGTGAAGA CCTTAACTAT 
AAACAAATTG CAAAAGAGCA AACAGGTGGC AGAATGGATC CATTTACCGA TAAGAATATA 
AAGCATTTGG TGCACAAGTT ACAGCAGGAA GGTTTATCTG GTCCTCTTTT GTGGCTTCAA 
AACTGTTTAA ACCGCACTGC CGAAGACAGA GAAGAGGATG GCTCTTCTCA GGCAATTCCC 
CTTGTGCCTT TAACGGAAGA CAATGAGGAT GCTATGGAAA ATAAAACATT CAAGAAACTG 
CTGAAGAAGT TGGGCATCCG TGCGCCTGCA AATGAGCAGG AATCTTTCTG GCGCATCCCA 
GGTAAATTAA GTCCGGAACA GCTGCGCAAG GCAGCCTCCT GTCTTGTTCC CTTGGCTGCA 
GCAGTTGCCT GTGAGGTTCT TGCAAATGCT GAAGAACAAG AGGTAAATGT TGAGCAGAGG 
GCAGAAGCAC TTCGTGCACT TTTACTTGCA CGGAAAAAGA AATCATCAGA AGCTCAGCAA 
GACCTTCAAG AAGAAAATCA GAAATCCCCA GATAAGTCAT TACAATCCCA AAGCAAAACC 
AGAAGACCAA AGACAGCAAA GAAGAGAAGT AAAATGGTTG ACAGTGATGA AGAGAATTCT 
GTAGACAATA CTAATGAGAA CAAGGAGTCA GAGCAGCTGC CAATGGATGC CATAACAGAG 
AGAGAAACCA AACCGAATAG ACCAAAGAAA TCAAAGAAAA GAAGTCGTAT AGTGGACAGT 
GATGATGACT CTGTGACAGA AGAGCATGGC AAGGAGATGT TAGAGGTAGA CCAGAAAGGA 
TCGTTTTCTG AATCGGACAC TGAGGAAAAT GTTCAACCTA CCAAGAAAAA GCGTCTTGTC 
ATTGAGGATG AAGATGAAGA TTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031753027.1
CDS	153..4016
Translation	MDLYMMNCELLATCSALGYLESDTYHKEPDCLESVKDLIRYLRHEDDTRDIRQQLGAAQILQNDLIPLVIQHHNDKQLFDAVIRLMVNVTQPALLCFGKVPQDPTFRHHFLQVVSYLQAYKEAFANERLFGVLSEKLYDLLQMEWEQREEEHNLLIERILLLVRNILHVPPDPEEEKSVDDDASIHDRVLWAIHMSGMDDLIKFLASSETEQQWSMHVLETVSLMFRDQIAEHLAATGQARSTAERDTDIKNLEILRQRESAEKKNRALQRGTRHSRFGGSYIVQGLKSIGDKDVIYHKGIHNFKNYTHDLGKEVRRVPKRKQQAKETEARRRSALNVRLFLKEFCIDFLENCYNRLMYIVKDNLIREKAQQHDETYYLWSMAFFMGFNRLFRFQPELVSETVSIRTFHFIEQNLTNYYEMMLTDKKEATSWARRMHLALKAYQELLSTVHEMDHSKEEAVRESSKIIKNNIFYLMEYRELFLALFRKFDETKQPRSFLKDLVETTHLFLKMLEKFCKGKQTVVVQKRRVKRKKKKSSVSGAKEVQPKSTEELAAIWTNVSEQLTTCIVNSENLPDSVVPFDAASEIPVEEQRAEALVRIQDGLFSGCAAEALVLLRSAREVWPEGDVFGAAEIEPEEETELLKQILFAELPRSTQAESEVLGEEEEEDEIEEEELTSVQVSEREFNFLDYMKRFANSNVVKTYVLLLKNYQQNSAHTNHCVAKMLHRIAYDLKMEALLFQLSVFCIFNKILGDPAASAYKELVAFSKYILNKFFSLAATNNKAYVELLFWKNTNVIREMTEGYRKLGEEESEANRKRIKWTPEDEDELRRLYLQFKEIEDADVVDNIVANLKPGTRTRKQVINHLVQMGLADSVKDFKQETKGTRIVLWTEDQELELQRLYEDFKDSDDILGNIMKNVTAKRSKARIIEKLISMGLVCDRKELYKKRRKKGKSSGMSEEEFLNDLGFDGKHSVDDVEEEEEEEEEEASEEEEECEDLNYKQIAKEQTGGRMDPFTDKNIKHLVHKLQQEGLSGPLLWLQNCLNRTAEDREEDGSSQAIPLVPLTEDNEDAMENKTFKKLLKKLGIRAPANEQESFWRIPGKLSPEQLRKAASCLVPLAAAVACEVLANAEEQEVNVEQRAEALRALLLARKKKSSEAQQDLQEENQKSPDKSLQSQSKTRRPKTAKKRSKMVDSDEENSVDNTNENKESEQLPMDAITERETKPNRPKKSKKRSRIVDSDDDSVTEEHGKEMLEVDQKGSFSESDTEENVQPTKKKRLVIEDEDED

Target ORF information:

RefSeq Version	XM_031897167.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis timeless circadian regulator (timeless), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031897167.1

ORF Insert Sequence:

ATGGACTTGT ACATGATGAA CTGTGAATTG TTAGCAACGT GCAGTGCCTT GGGGTACCTG 
GAGAGCGATA CCTACCACAA AGAGCCTGAC TGTCTTGAGA GTGTAAAAGA TTTAATTCGT 
TACTTGCGCC ATGAGGATGA TACAAGAGAT ATCAGACAGC AGCTTGGGGC TGCCCAGATA 
CTGCAAAATG ATCTTATTCC CCTGGTTATC CAGCATCACA ATGATAAACA GCTGTTTGAT 
GCTGTAATCA GGTTGATGGT AAATGTAACG CAGCCGGCAC TTTTGTGCTT TGGGAAAGTA 
CCTCAGGATC CTACATTTAG ACACCATTTT CTGCAGGTTG TTTCCTATTT ACAAGCTTAC 
AAGGAGGCAT TTGCAAATGA AAGACTATTT GGAGTCTTAA GTGAAAAATT ATATGATTTA 
CTGCAGATGG AATGGGAGCA ACGAGAGGAG GAACATAATT TACTTATAGA AAGGATTCTT 
CTGCTAGTGC GTAATATTCT CCATGTGCCC CCTGACCCAG AGGAGGAGAA AAGTGTAGAT 
GACGATGCTA GTATTCATGA CCGAGTGCTT TGGGCAATCC ACATGAGTGG AATGGATGAT 
CTAATTAAGT TTTTGGCTAG CTCAGAGACA GAGCAACAAT GGAGCATGCA TGTGCTGGAG 
ACTGTATCTC TTATGTTTAG AGACCAGATA GCAGAGCATT TAGCTGCAAC AGGCCAGGCA 
CGCTCAACTG CAGAGCGAGA TACTGATATT AAAAACTTGG AGATCCTGAG ACAGAGGGAA 
TCAGCAGAAA AGAAGAACAG GGCTCTTCAG CGTGGGACTC GGCATTCTCG GTTTGGTGGT 
TCTTACATTG TTCAGGGACT GAAATCTATT GGAGACAAAG ATGTAATCTA TCACAAAGGC 
ATTCACAATT TCAAGAACTA CACTCATGAC CTTGGAAAGG AAGTGCGGAG AGTCCCAAAG 
AGAAAGCAGC AGGCCAAGGA AACAGAGGCA CGACGCCGCT CTGCACTCAA TGTTCGATTA 
TTTCTAAAGG AGTTTTGCAT TGACTTTCTT GAAAATTGCT ACAATCGACT CATGTACATT 
GTAAAAGATA ATCTGATTCG TGAGAAGGCA CAGCAGCATG ACGAAACCTA CTACTTGTGG 
TCCATGGCTT TTTTTATGGG GTTTAATCGC CTCTTCCGCT TCCAACCTGA GCTTGTGTCT 
GAGACAGTTA GCATCAGGAC CTTCCACTTC ATAGAGCAGA ACCTCACAAA TTATTATGAG 
ATGATGCTGA CTGACAAGAA GGAGGCCACA TCATGGGCCC GGCGAATGCA CCTTGCTCTA 
AAAGCGTATC AAGAGCTTCT AAGCACTGTT CATGAGATGG ACCATTCTAA GGAAGAGGCT 
GTGAGGGAAA GCAGCAAAAT CATTAAAAAT AACATATTCT ATTTAATGGA GTACCGAGAG 
CTCTTTCTGG CGCTATTCAG GAAATTTGAT GAGACTAAGC AGCCACGTTC CTTCTTAAAG 
GATCTGGTGG AGACAACACA TTTGTTTCTT AAAATGTTAG AAAAATTTTG CAAAGGAAAA 
CAGACTGTGG TCGTACAGAA ACGCCGTGTG AAACGAAAGA AGAAGAAATC CTCTGTAAGT 
GGTGCTAAGG AGGTTCAGCC TAAGAGCACA GAGGAGCTGG CGGCAATATG GACAAATGTT 
TCAGAGCAGC TCACGACCTG CATAGTGAAT TCAGAAAATC TACCTGACAG TGTTGTGCCT 
TTTGATGCTG CCTCTGAGAT ACCAGTAGAA GAGCAGAGGG CAGAAGCCTT AGTACGAATC 
CAAGATGGTC TCTTCTCTGG CTGTGCAGCA GAGGCCCTTG TTCTGCTGCG TTCTGCTAGG 
GAGGTCTGGC CAGAGGGGGA TGTTTTTGGT GCTGCAGAGA TAGAACCTGA AGAAGAGACT 
GAGCTACTGA AACAAATTCT CTTTGCAGAG CTTCCCAGAT CAACACAGGC TGAAAGTGAG 
GTTCTGGGAG AGGAAGAAGA GGAAGATGAG ATTGAGGAAG AAGAACTTAC CTCAGTTCAA 
GTATCTGAGA GGGAATTTAA CTTTCTAGAT TACATGAAAC GGTTTGCTAA CTCCAATGTA 
GTGAAAACCT ATGTCCTGCT GCTGAAGAAT TACCAACAGA ACAGTGCTCA CACCAATCAC 
TGTGTAGCGA AGATGTTGCA CCGTATTGCA TATGACCTCA AAATGGAGGC ACTTCTCTTT 
CAGCTTTCAG TGTTTTGTAT TTTCAACAAA ATTCTGGGTG ATCCAGCAGC AAGTGCCTAT 
AAGGAGTTGG TGGCCTTTTC TAAATACATC TTAAATAAGT TCTTTTCCCT GGCGGCAACA 
AACAACAAGG CCTATGTGGA GCTTCTTTTC TGGAAAAACA CCAATGTGAT TAGAGAAATG 
ACAGAGGGCT ATAGAAAACT TGGAGAAGAA GAGAGTGAAG CGAACCGCAA GCGCATAAAG 
TGGACTCCAG AGGATGAGGA TGAGCTGAGG AGACTTTATT TGCAGTTCAA AGAGATAGAA 
GACGCAGATG TGGTGGATAA TATCGTCGCC AACTTAAAAC CTGGAACCCG TACTCGGAAG 
CAAGTCATTA ACCATTTGGT GCAAATGGGG CTAGCTGACA GTGTCAAGGA CTTCAAACAA 
GAAACAAAAG GAACTCGCAT TGTGCTATGG ACAGAGGATC AAGAGCTGGA ACTTCAGCGC 
CTTTATGAAG ACTTTAAAGA CTCAGATGAC ATATTGGGAA ATATAATGAA GAATGTGACT 
GCAAAACGCT CCAAGGCTAG AATAATTGAG AAGCTTATCA GCATGGGTCT AGTATGCGAT 
CGCAAAGAAC TTTATAAGAA AAGAAGGAAG AAGGGGAAAT CATCTGGCAT GTCTGAAGAA 
GAGTTTCTCA ATGATCTGGG GTTTGATGGA AAACACAGTG TGGATGATGT GGAAGAAGAG 
GAGGAGGAGG AAGAAGAAGA AGCAAGTGAA GAAGAAGAAG AGTGTGAAGA CCTTAACTAT 
AAACAAATTG CAAAAGAGCA AACAGGTGGC AGAATGGATC CATTTACCGA TAAGAATATA 
AAGCATTTGG TGCACAAGTT ACAGCAGGAA GGTTTATCTG GTCCTCTTTT GTGGCTTCAA 
AACTGTTTAA ACCGCACTGC CGAAGACAGA GAAGAGGATG GCTCTTCTCA GGCAATTCCC 
CTTGTGCCTT TAACGGAAGA CAATGAGGAT GCTATGGAAA ATAAAACATT CAAGAAACTG 
CTGAAGAAGT TGGGCATCCG TGCGCCTGCA AATGAGCAGG AATCTTTCTG GCGCATCCCA 
GGTAAATTAA GTCCGGAACA GCTGCGCAAG GCAGCCTCCT GTCTTGTTCC CTTGGCTGCA 
GCAGTTGCCT GTGAGGTTCT TGCAAATGCT GAAGAACAAG AGGTAAATGT TGAGCAGAGG 
GCAGAAGCAC TTCGTGCACT TTTACTTGCA CGGAAAAAGA AATCATCAGA AGCTCAGCAA 
GACCTTCAAG AAGAAAATCA GAAATCCCCA GATAAGTCAT TACAATCCCA AAGCAAAACC 
AGAAGACCAA AGACAGCAAA GAAGAGAAGT AAAATGGTTG ACAGTGATGA AGAGAATTCT 
GTAGACAATA CTAATGAGAA CAAGGAGTCA GAGCAGCTGC CAATGGATGC CATAACAGAG 
AGAGAAACCA AACCGAATAG ACCAAAGAAA TCAAAGAAAA GAAGTCGTAT AGTGGACAGT 
GATGATGACT CTGTGACAGA AGAGCATGGC AAGGAGATGT TAGAGGTAGA CCAGAAAGGA 
TCGTTTTCTG AATCGGACAC TGAGGAAAAT GTTCAACCTA CCAAGAAAAA GCGTCTTGTC 
ATTGAGGATG AAGATGAAGA TTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

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timeless cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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