ppl.L cDNA ORF clone, Xenopus laevis(African clawed frog)

Gene
Clones
gRNAs

Summary

Gene Symbol	ppl.L
Entrez Gene ID	495053
Full Name	periplakin L homeolog
Synonyms	ppl
General protein information	Preferred Names periplakin L homeolog Names periplakin
Gene Type	protein-coding
Organism	Xenopus laevis(African clawed frog)

mRNA and Protein(s)

mRNA	Protein	Name
XM_018236668.1	XP_018092157.1	periplakin

Pathways from BioSystems
Gene Ontology

The following ppl.L gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ppl.L cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OAf46940	XM_018236668.1 Latest version!	Xenopus laevis periplakin L homeolog (ppl.L), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

ppl.L ( XM_018236668.1 ) cDNA ORF clone, Xenopus laevis(African clawed frog) -> XP_018092157.1 Xenopus laevis periplakin L homeolog (ppl.L), mRNA.

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CloneID	OAf46940
Clone ID Related Accession (Same CDS sequence)	XM_018236668.1
Accession Version	XM_018236668.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5295bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1474214400000
Organism	Xenopus laevis(African clawed frog)
Product	periplakin
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030740.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus laevis Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGTTCCT TGTTTAAGAA ACGAAACAAA TACAGTCCCA ATACTCAACC ACCACCACCA 
CCACCATCCA AAGTTGTCTC CAACAAGGAG CTGTCAGAGC TGATCGAGAG GCTTCAGAAA 
AATGCAGATC AAGTGGAACG CAACGTGGTG GACACAGACA CCAAACTGCA GGAGGACGCG 
GATAAGCTTG CTGCCTCCAG ACCACTAAGC TACAAGGATT ACACCACCAA CACCATCAAG 
GAATCAGAGA AAATTGTCGT GGTACTGGAG ACAGATGCTG TGCAGGCACG CCAGCTCAAG 
CACCCACAGG CCGACATGAT TAGTGAGGAC ATTCGCCAAC TAAAGGAACG GATTCAGGCC 
CAGAAAAAGA AGCACGACCA CGTTTATAAC CAGAAGATAC CTAAGGGAGA AGTTCCAAGA 
TTCGACTGGG GCAAATTGAT TGATAGTAAT ATGGATGAGC TGAACAACCA GGGGTTTGCA 
ACAGATTTGC CCAACCTGGA GAAACAGATT GAAGAGCATA ACATCTTCCA CAATGAGGTC 
AAGACCATTG GGGATCAAGT CAACAAGAAT AATGACAAGG AATACATTAG TGGTCTGCAG 
GTGAAATACC AAAAGCTTTT GTCTGCATCC CAGAAGCGCC AGAGGGATCT CAGTACCCTG 
CAGGATTACA TGCAACGTTG CACCAATGAA CTGTACTGGC TGGATCAGCA GGAGAAGGAA 
CGCATGGAGT ATGATTGGAG CGATCACAAC AAGGACTACA TAAGCCGTCG GAGGCAATAC 
GAGAACTTCA TCCAGCATAG TCTGGAGACA AAGGAGCATG AAATCAGCAA GCTTCAGGAA 
GAAGGCGACA AGCTCATTGC ATCAGGACAC CCTGGAAAAA TATCAATTGA GGCCCATACA 
GAAGCAGTTC ATGCAGACTG GAAGGAGTAT TTGAACCTCC TGATTTGTGA GGAGAGTCAC 
CTGAAGTACA TGGAGGATTA TCATCAGTTC TACAGAGACG CAAAAGATGC TCAGGATCTG 
CTAAAGAAAG TTGACGGTGA TATAGACCAT AAGTATAACC CAGACTACAA GGACCGTTAC 
CAGATTGAAG CTCAGCTACG TGAGCTGGAG GATCAGGACA AGGCTTTAGA TAAGTACGAG 
GAAGTCATAC AATCTCTGCA AAAGCGCAGC CAGCAGGTGG TGCCACTAAA ATTCCGCAGG 
GAGACTCCAT TGAAACCAAT CCCAGTGGAA TCCTTGTGTG ACTTCTACTC TGAAGAGGGT 
GAGATTAGCC GTGGAGTGAG TTATACTCTG AAGGCCAATA GAGGGGAGAA ATGGGAGTTG 
ACTGACAGCA ATGGAAAGAA ATTGGTTGCA CCATCAGTAT GCTTCACAAT CCCACCAACT 
GACCCTGAGG CTGTAGCACT TGGAGAAAGC ATGGTCAACC AATACCGAGC AACAAAGCAA 
AAATCAGTCG GATCAAAAAA TGCATTGCTG CAGCACTTAG ATGTGCAAAA GAAGGAAAGC 
AGTCAAGTCG CTGCAAATGC CCAGGATACT CAAGGGCGTC AACTGCTTGC TGGATTGGAC 
AAAGTCCAAA GTGATTTGGA AAGGCAAGAG AAAGCCATAA GCTCCCAACT GCGCCCACCT 
CTAGAGCAGA TCAGAGCCGT GCAGGACAGC TCAGAGAGGC TGAAGGAGCT CAAGAATATC 
TCAAACCAAG TACTGCGCAT TGAACCTGAA AAGGTCCAGA AGGTCCAAGA GTGTGAGGCA 
TTCCTGCAGA AATCCCCTGA TGTCTCTTGC TCACCCATCT TGATGAGCAA AATGGGAGAA 
ACCAACAAGA AATATGAAAG GGTCAATGAT CTTCTCTTAT CGGCTCAGGA GAAGATAGAC 
GGGTCCAATC GCTTGGAAGG CAGCCTGCAG AAGGGCAGAG ACCTTCTCTC CAGCTATGAA 
ACCAAACTTG CCCTAGACGA CACAGTGCCA GAAGATCCAC AAGCCATTGA CAGGAAACTA 
CAGGAGTTAT CGGCAACAAG CTCCGAGCTG CAGAAGAGCA GGGCGGTTTT TGCAGAGGCC 
GAACAGAACC TGTACAGCAG TCGTACATGT TCCAACAACC TGGCCAACCG CTTTCAAGAG 
CACTGTCCTG ACATTGATCG CCAAGAGGCT GAAGTGTCCA AACTCAACCA GCGTTACACC 
CAGTTGGACC AGCAGATCCA GAACAGGTCT CAAAGCCTGC AGAAAGCCAA AGTCGCCTAC 
TCCGGTTACC GCAACAACTA TGACCATATT GATCAGTGGC TACTGAATCT TCCCAACTAT 
GAGCCCAAGG ACACAGACAG TGTCAAGCAA GTGGAGACCA AACTGAAAAA CCAGAAACTT 
CTAGTGACTG AGATTGCAGA CAAAGAGCAG GACATGCAGC ACGTATCAAG CAGCGCCCAG 
CAGTACCAAC AAGCAGTGAA GGATTATGAG CTTGAAACTG AGAAGTTGAG GTCAATTCTG 
GACACAGAAA ACAGAAGGAA CAGTTATTCT AAAAGGCCTA AAATCCAGTC ACCAGGTGCC 
AAAGTGAAGG AACAGGAAGC TGATTTGTCA GCAAGATACA CTGAAGCCAA TGCGGTGAAC 
AAGCAGAGGT TACAGACACT GGAGTTTGCC CAAAGCCTTC TCCGGCAGCA ACCGGAAGCA 
GAAATACGTC AAGAGTCAGT TCAGATGATC CGTTCTGAGA AACCAGCTGA GGAGACCTGG 
AAAATCAAAA ATATGTTAGA TGATGAAGTG CAGCGCCGGC AGCAGTTGGA ATCAGAGATC 
AAATCCACTC AAGATGAGAT AATCGTTTTA CAGAACCAAA AACCTCAGGA GACCCTAGTG 
AAAAAGGAAA TGTTGAAGAA GGTACCAGAT CCACAGCTAG AAGATGAATA TCGCAAGGTT 
ATTGAGAGAG TCTCAGAAGA GCAGCGCTTA AACAAGTCTT TGCAAGATGA GCTGGAATCC 
CTGAAGTTGA AAGTCCGTGC ACTGGAATAT GAGCAAAGAG AAGGTGGTCA ACAATACAGA 
GTCAAGGAAG TCCTGAGGAT AGAAAAAGAT AAAGCACAGC AGGAAGAATG CTTGAGACTC 
AATGAAGAGT TGGAATGGCT GAAGAGGCAA AAAGGGGCCA AACAGAGTGA GATAACAAGC 
TTAGAGGAAC GTAAACTTAT CTTGTCAGAA CAAAAAAATA AACAGCAGGA GAAAATCACA 
GAGAAAGAGG TTGTCAAAAT GCAGAGTGAC CCACAACTGG CTTTGGAATA TAAACGTCTT 
CAGGAGAATA AACAAAAGGA AAGTGCTCAA AGGCAGAAGG AGGAAGAAGA GCTGATGCTC 
CTGCAGGAGA AGTTAAAACG CCTAGAGAAA GAGAGAGCTA TGGCTGAGGG CAAGATCACA 
GTAAAAGAAG TGCTAAAAGT TGAGAAAGAT CTGGCCACTG AGAGGGAGGT GGCTGACCTT 
CGTAGACAGT ATGACGAAGA GACTTCCAAG ACACGAACTG CCCAGAGGGA AAAGAATGAG 
TTCATTCGAA AGATAAATAA TTTAGAAGAA GAGAATGCAA AAGTTGTGGT TCAGGAGAAA 
GTACGTGAGA TTGTTCGCCC AGACCCAAAA GCTGAAAGTG AACTGGCCAA CCTTCGCCTG 
GAGGTGATAG AGCAAGAGAG AAAATACAGA GCAACCGAAG AGCAACTGAA AAGTGTGCAG 
AGCGAACTGA CAGTCTTACA AAAGCGTGGT CCACAAGTAG AGACAAAAGA AATCATCAAG 
GAAGTCATTA CGCACAAGAC AGACCCTGAG GTTTTGTCAG AACTGGAGAA ACTCCGTGGT 
GAGATCTTTA GCAAGACACG AATCATAGAG CGTTCGGAGC TAGAGATCAC ACAGCTAAAT 
CAGGAGATTC AAAGCTGGAA AGATACAAAG CCACAAGTTC AGATCAAGGA GGTTATTCAA 
GAGGTCCTTC AGTACCGTGA AGACCCAACC ACAAAGAAAG AAGTAGAGAC ATTGAGAGAA 
AAGCTGGCTG AGGAACAGAA GAAATGCTTG GAACTAGAAA AGGAGAGACT TTCTCAAGAG 
GACAAAATAA GAAAGAAAGA AATGGAACTT TCACAAGTGA AAGAAAAGGT GGTTCAACAA 
GAGGTGGTGA AGCTTGAAGA GGATCCTACT CTGAAGGCAG AAGTTAACAG TTTCTCCAAA 
ACCATTGAGA CTGAGCTGAA GCAAAAGGAC TCTCTGCAGG AGGAACTACG CAAGCTGCAG 
TGGCGGAAAT CAGAGCTGGA ACGTCAGTTT GAGGAGCTAG AAAAGGAGAG GCAGGCTAGG 
CGAGAGGCAG AGCTTGAGAT AAATAAACTG AAATACAAGT TGACTGAACT GGAACAGAGA 
GAAATAGAGA CCAAAGAGAA AGTTACATTG CAACAGAAAG TTGTCATGCA ACAAGACCCA 
CAACAAGAAA AGGAGCATAA CCTCCTGAAG CTCCAAATGG AAGAAGAAAA GCACAAGCGG 
CACATGAGGG AGGGTGAACT AGAAGCATTG CAGAAAAAAC TCATCTCATT AGAGAAGATG 
GAGCTAAAGG AAAAGGTTGT TTTTACAGAG AAAATAGAGG TAGAAAAAGA TCCTGAAACA 
GAGCAAGAGA TACAGAGGCT GAAAATCAGC TTGGAGGAAG AGAGTAAATC CAAGAGAGAG 
CTGGACTCGG AGGTAAGCCG TCTTCAAACA CGCCTTTCGG AAATTGAATT CAACAACTCT 
AAGGCCAACA AAGAGCTTGA ATACCTAAGG GATGAGGTGC ATAAGCTACA GATGGAAAAA 
CAAAATCTGC AGCTGGAGAC CCGCCACCTG CATTCGGAAA TTCAGATTAC CATGAAGGAA 
GCTCAAGATA TCCGAAACAT GACTCAAGTT GACAGCAATG TGAACCTGGA TTCCCGCCTC 
AATGCTCTGG AAAAAGAGCT GAAAGAACTC CAAAAGATCT CCAAAGACAA AGATGCTGAG 
ATCGAGCAAC TGCAAAAGCG CCTGGAAAGC ATGACAATCA AGAAGGAACA GAGGGAGAAC 
CACCTCCGCC GTTCCATTGT CGTCATTGAC CCCGACACAG GTAGAGAGAT GTCCCCTGAG 
GAAGCCCGTA GACTGGGTCT CATCGACTGG AATATGTTTG TCAACCTTCA GAACCAAGAG 
TGCGACTGGG AAGAAACGAC CATCAAGGGG CCCAATGGAG AGTCTTCTGT GATTATTGAT 
AGAAAATCTG GCAGGAAGTT CGACATTGAA GAGGCCCTGA GACTGCGACG CATCACCAGA 
CGCCAGTACG ACTGTTACCT CAACAAGGAA ATGTCCATCC AGGAATTTGC CGCCCTGGTC 
TCTGGGAGGC TGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_018092157.1
CDS	243..5537
Translation	MSSLFKKRNKYSPNTQPPPPPPSKVVSNKELSELIERLQKNADQVERNVVDTDTKLQEDADKLAASRPLSYKDYTTNTIKESEKIVVVLETDAVQARQLKHPQADMISEDIRQLKERIQAQKKKHDHVYNQKIPKGEVPRFDWGKLIDSNMDELNNQGFATDLPNLEKQIEEHNIFHNEVKTIGDQVNKNNDKEYISGLQVKYQKLLSASQKRQRDLSTLQDYMQRCTNELYWLDQQEKERMEYDWSDHNKDYISRRRQYENFIQHSLETKEHEISKLQEEGDKLIASGHPGKISIEAHTEAVHADWKEYLNLLICEESHLKYMEDYHQFYRDAKDAQDLLKKVDGDIDHKYNPDYKDRYQIEAQLRELEDQDKALDKYEEVIQSLQKRSQQVVPLKFRRETPLKPIPVESLCDFYSEEGEISRGVSYTLKANRGEKWELTDSNGKKLVAPSVCFTIPPTDPEAVALGESMVNQYRATKQKSVGSKNALLQHLDVQKKESSQVAANAQDTQGRQLLAGLDKVQSDLERQEKAISSQLRPPLEQIRAVQDSSERLKELKNISNQVLRIEPEKVQKVQECEAFLQKSPDVSCSPILMSKMGETNKKYERVNDLLLSAQEKIDGSNRLEGSLQKGRDLLSSYETKLALDDTVPEDPQAIDRKLQELSATSSELQKSRAVFAEAEQNLYSSRTCSNNLANRFQEHCPDIDRQEAEVSKLNQRYTQLDQQIQNRSQSLQKAKVAYSGYRNNYDHIDQWLLNLPNYEPKDTDSVKQVETKLKNQKLLVTEIADKEQDMQHVSSSAQQYQQAVKDYELETEKLRSILDTENRRNSYSKRPKIQSPGAKVKEQEADLSARYTEANAVNKQRLQTLEFAQSLLRQQPEAEIRQESVQMIRSEKPAEETWKIKNMLDDEVQRRQQLESEIKSTQDEIIVLQNQKPQETLVKKEMLKKVPDPQLEDEYRKVIERVSEEQRLNKSLQDELESLKLKVRALEYEQREGGQQYRVKEVLRIEKDKAQQEECLRLNEELEWLKRQKGAKQSEITSLEERKLILSEQKNKQQEKITEKEVVKMQSDPQLALEYKRLQENKQKESAQRQKEEEELMLLQEKLKRLEKERAMAEGKITVKEVLKVEKDLATEREVADLRRQYDEETSKTRTAQREKNEFIRKINNLEEENAKVVVQEKVREIVRPDPKAESELANLRLEVIEQERKYRATEEQLKSVQSELTVLQKRGPQVETKEIIKEVITHKTDPEVLSELEKLRGEIFSKTRIIERSELEITQLNQEIQSWKDTKPQVQIKEVIQEVLQYREDPTTKKEVETLREKLAEEQKKCLELEKERLSQEDKIRKKEMELSQVKEKVVQQEVVKLEEDPTLKAEVNSFSKTIETELKQKDSLQEELRKLQWRKSELERQFEELEKERQARREAELEINKLKYKLTELEQREIETKEKVTLQQKVVMQQDPQQEKEHNLLKLQMEEEKHKRHMREGELEALQKKLISLEKMELKEKVVFTEKIEVEKDPETEQEIQRLKISLEEESKSKRELDSEVSRLQTRLSEIEFNNSKANKELEYLRDEVHKLQMEKQNLQLETRHLHSEIQITMKEAQDIRNMTQVDSNVNLDSRLNALEKELKELQKISKDKDAEIEQLQKRLESMTIKKEQRENHLRRSIVVIDPDTGREMSPEEARRLGLIDWNMFVNLQNQECDWEETTIKGPNGESSVIIDRKSGRKFDIEEALRLRRITRRQYDCYLNKEMSIQEFAALVSGRL

Target ORF information:

RefSeq Version	XM_018236668.1
Organism	Xenopus laevis(African clawed frog)
Definition	Xenopus laevis periplakin L homeolog (ppl.L), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_018236668.1

ORF Insert Sequence:

ATGAGTTCCT TGTTTAAGAA ACGAAACAAA TACAGTCCCA ATACTCAACC ACCACCACCA 
CCACCATCCA AAGTTGTCTC CAACAAGGAG CTGTCAGAGC TGATCGAGAG GCTTCAGAAA 
AATGCAGATC AAGTGGAACG CAACGTGGTG GACACAGACA CCAAACTGCA GGAGGACGCG 
GATAAGCTTG CTGCCTCCAG ACCACTAAGC TACAAGGATT ACACCACCAA CACCATCAAG 
GAATCAGAGA AAATTGTCGT GGTACTGGAG ACAGATGCTG TGCAGGCACG CCAGCTCAAG 
CACCCACAGG CCGACATGAT TAGTGAGGAC ATTCGCCAAC TAAAGGAACG GATTCAGGCC 
CAGAAAAAGA AGCACGACCA CGTTTATAAC CAGAAGATAC CTAAGGGAGA AGTTCCAAGA 
TTCGACTGGG GCAAATTGAT TGATAGTAAT ATGGATGAGC TGAACAACCA GGGGTTTGCA 
ACAGATTTGC CCAACCTGGA GAAACAGATT GAAGAGCATA ACATCTTCCA CAATGAGGTC 
AAGACCATTG GGGATCAAGT CAACAAGAAT AATGACAAGG AATACATTAG TGGTCTGCAG 
GTGAAATACC AAAAGCTTTT GTCTGCATCC CAGAAGCGCC AGAGGGATCT CAGTACCCTG 
CAGGATTACA TGCAACGTTG CACCAATGAA CTGTACTGGC TGGATCAGCA GGAGAAGGAA 
CGCATGGAGT ATGATTGGAG CGATCACAAC AAGGACTACA TAAGCCGTCG GAGGCAATAC 
GAGAACTTCA TCCAGCATAG TCTGGAGACA AAGGAGCATG AAATCAGCAA GCTTCAGGAA 
GAAGGCGACA AGCTCATTGC ATCAGGACAC CCTGGAAAAA TATCAATTGA GGCCCATACA 
GAAGCAGTTC ATGCAGACTG GAAGGAGTAT TTGAACCTCC TGATTTGTGA GGAGAGTCAC 
CTGAAGTACA TGGAGGATTA TCATCAGTTC TACAGAGACG CAAAAGATGC TCAGGATCTG 
CTAAAGAAAG TTGACGGTGA TATAGACCAT AAGTATAACC CAGACTACAA GGACCGTTAC 
CAGATTGAAG CTCAGCTACG TGAGCTGGAG GATCAGGACA AGGCTTTAGA TAAGTACGAG 
GAAGTCATAC AATCTCTGCA AAAGCGCAGC CAGCAGGTGG TGCCACTAAA ATTCCGCAGG 
GAGACTCCAT TGAAACCAAT CCCAGTGGAA TCCTTGTGTG ACTTCTACTC TGAAGAGGGT 
GAGATTAGCC GTGGAGTGAG TTATACTCTG AAGGCCAATA GAGGGGAGAA ATGGGAGTTG 
ACTGACAGCA ATGGAAAGAA ATTGGTTGCA CCATCAGTAT GCTTCACAAT CCCACCAACT 
GACCCTGAGG CTGTAGCACT TGGAGAAAGC ATGGTCAACC AATACCGAGC AACAAAGCAA 
AAATCAGTCG GATCAAAAAA TGCATTGCTG CAGCACTTAG ATGTGCAAAA GAAGGAAAGC 
AGTCAAGTCG CTGCAAATGC CCAGGATACT CAAGGGCGTC AACTGCTTGC TGGATTGGAC 
AAAGTCCAAA GTGATTTGGA AAGGCAAGAG AAAGCCATAA GCTCCCAACT GCGCCCACCT 
CTAGAGCAGA TCAGAGCCGT GCAGGACAGC TCAGAGAGGC TGAAGGAGCT CAAGAATATC 
TCAAACCAAG TACTGCGCAT TGAACCTGAA AAGGTCCAGA AGGTCCAAGA GTGTGAGGCA 
TTCCTGCAGA AATCCCCTGA TGTCTCTTGC TCACCCATCT TGATGAGCAA AATGGGAGAA 
ACCAACAAGA AATATGAAAG GGTCAATGAT CTTCTCTTAT CGGCTCAGGA GAAGATAGAC 
GGGTCCAATC GCTTGGAAGG CAGCCTGCAG AAGGGCAGAG ACCTTCTCTC CAGCTATGAA 
ACCAAACTTG CCCTAGACGA CACAGTGCCA GAAGATCCAC AAGCCATTGA CAGGAAACTA 
CAGGAGTTAT CGGCAACAAG CTCCGAGCTG CAGAAGAGCA GGGCGGTTTT TGCAGAGGCC 
GAACAGAACC TGTACAGCAG TCGTACATGT TCCAACAACC TGGCCAACCG CTTTCAAGAG 
CACTGTCCTG ACATTGATCG CCAAGAGGCT GAAGTGTCCA AACTCAACCA GCGTTACACC 
CAGTTGGACC AGCAGATCCA GAACAGGTCT CAAAGCCTGC AGAAAGCCAA AGTCGCCTAC 
TCCGGTTACC GCAACAACTA TGACCATATT GATCAGTGGC TACTGAATCT TCCCAACTAT 
GAGCCCAAGG ACACAGACAG TGTCAAGCAA GTGGAGACCA AACTGAAAAA CCAGAAACTT 
CTAGTGACTG AGATTGCAGA CAAAGAGCAG GACATGCAGC ACGTATCAAG CAGCGCCCAG 
CAGTACCAAC AAGCAGTGAA GGATTATGAG CTTGAAACTG AGAAGTTGAG GTCAATTCTG 
GACACAGAAA ACAGAAGGAA CAGTTATTCT AAAAGGCCTA AAATCCAGTC ACCAGGTGCC 
AAAGTGAAGG AACAGGAAGC TGATTTGTCA GCAAGATACA CTGAAGCCAA TGCGGTGAAC 
AAGCAGAGGT TACAGACACT GGAGTTTGCC CAAAGCCTTC TCCGGCAGCA ACCGGAAGCA 
GAAATACGTC AAGAGTCAGT TCAGATGATC CGTTCTGAGA AACCAGCTGA GGAGACCTGG 
AAAATCAAAA ATATGTTAGA TGATGAAGTG CAGCGCCGGC AGCAGTTGGA ATCAGAGATC 
AAATCCACTC AAGATGAGAT AATCGTTTTA CAGAACCAAA AACCTCAGGA GACCCTAGTG 
AAAAAGGAAA TGTTGAAGAA GGTACCAGAT CCACAGCTAG AAGATGAATA TCGCAAGGTT 
ATTGAGAGAG TCTCAGAAGA GCAGCGCTTA AACAAGTCTT TGCAAGATGA GCTGGAATCC 
CTGAAGTTGA AAGTCCGTGC ACTGGAATAT GAGCAAAGAG AAGGTGGTCA ACAATACAGA 
GTCAAGGAAG TCCTGAGGAT AGAAAAAGAT AAAGCACAGC AGGAAGAATG CTTGAGACTC 
AATGAAGAGT TGGAATGGCT GAAGAGGCAA AAAGGGGCCA AACAGAGTGA GATAACAAGC 
TTAGAGGAAC GTAAACTTAT CTTGTCAGAA CAAAAAAATA AACAGCAGGA GAAAATCACA 
GAGAAAGAGG TTGTCAAAAT GCAGAGTGAC CCACAACTGG CTTTGGAATA TAAACGTCTT 
CAGGAGAATA AACAAAAGGA AAGTGCTCAA AGGCAGAAGG AGGAAGAAGA GCTGATGCTC 
CTGCAGGAGA AGTTAAAACG CCTAGAGAAA GAGAGAGCTA TGGCTGAGGG CAAGATCACA 
GTAAAAGAAG TGCTAAAAGT TGAGAAAGAT CTGGCCACTG AGAGGGAGGT GGCTGACCTT 
CGTAGACAGT ATGACGAAGA GACTTCCAAG ACACGAACTG CCCAGAGGGA AAAGAATGAG 
TTCATTCGAA AGATAAATAA TTTAGAAGAA GAGAATGCAA AAGTTGTGGT TCAGGAGAAA 
GTACGTGAGA TTGTTCGCCC AGACCCAAAA GCTGAAAGTG AACTGGCCAA CCTTCGCCTG 
GAGGTGATAG AGCAAGAGAG AAAATACAGA GCAACCGAAG AGCAACTGAA AAGTGTGCAG 
AGCGAACTGA CAGTCTTACA AAAGCGTGGT CCACAAGTAG AGACAAAAGA AATCATCAAG 
GAAGTCATTA CGCACAAGAC AGACCCTGAG GTTTTGTCAG AACTGGAGAA ACTCCGTGGT 
GAGATCTTTA GCAAGACACG AATCATAGAG CGTTCGGAGC TAGAGATCAC ACAGCTAAAT 
CAGGAGATTC AAAGCTGGAA AGATACAAAG CCACAAGTTC AGATCAAGGA GGTTATTCAA 
GAGGTCCTTC AGTACCGTGA AGACCCAACC ACAAAGAAAG AAGTAGAGAC ATTGAGAGAA 
AAGCTGGCTG AGGAACAGAA GAAATGCTTG GAACTAGAAA AGGAGAGACT TTCTCAAGAG 
GACAAAATAA GAAAGAAAGA AATGGAACTT TCACAAGTGA AAGAAAAGGT GGTTCAACAA 
GAGGTGGTGA AGCTTGAAGA GGATCCTACT CTGAAGGCAG AAGTTAACAG TTTCTCCAAA 
ACCATTGAGA CTGAGCTGAA GCAAAAGGAC TCTCTGCAGG AGGAACTACG CAAGCTGCAG 
TGGCGGAAAT CAGAGCTGGA ACGTCAGTTT GAGGAGCTAG AAAAGGAGAG GCAGGCTAGG 
CGAGAGGCAG AGCTTGAGAT AAATAAACTG AAATACAAGT TGACTGAACT GGAACAGAGA 
GAAATAGAGA CCAAAGAGAA AGTTACATTG CAACAGAAAG TTGTCATGCA ACAAGACCCA 
CAACAAGAAA AGGAGCATAA CCTCCTGAAG CTCCAAATGG AAGAAGAAAA GCACAAGCGG 
CACATGAGGG AGGGTGAACT AGAAGCATTG CAGAAAAAAC TCATCTCATT AGAGAAGATG 
GAGCTAAAGG AAAAGGTTGT TTTTACAGAG AAAATAGAGG TAGAAAAAGA TCCTGAAACA 
GAGCAAGAGA TACAGAGGCT GAAAATCAGC TTGGAGGAAG AGAGTAAATC CAAGAGAGAG 
CTGGACTCGG AGGTAAGCCG TCTTCAAACA CGCCTTTCGG AAATTGAATT CAACAACTCT 
AAGGCCAACA AAGAGCTTGA ATACCTAAGG GATGAGGTGC ATAAGCTACA GATGGAAAAA 
CAAAATCTGC AGCTGGAGAC CCGCCACCTG CATTCGGAAA TTCAGATTAC CATGAAGGAA 
GCTCAAGATA TCCGAAACAT GACTCAAGTT GACAGCAATG TGAACCTGGA TTCCCGCCTC 
AATGCTCTGG AAAAAGAGCT GAAAGAACTC CAAAAGATCT CCAAAGACAA AGATGCTGAG 
ATCGAGCAAC TGCAAAAGCG CCTGGAAAGC ATGACAATCA AGAAGGAACA GAGGGAGAAC 
CACCTCCGCC GTTCCATTGT CGTCATTGAC CCCGACACAG GTAGAGAGAT GTCCCCTGAG 
GAAGCCCGTA GACTGGGTCT CATCGACTGG AATATGTTTG TCAACCTTCA GAACCAAGAG 
TGCGACTGGG AAGAAACGAC CATCAAGGGG CCCAATGGAG AGTCTTCTGT GATTATTGAT 
AGAAAATCTG GCAGGAAGTT CGACATTGAA GAGGCCCTGA GACTGCGACG CATCACCAGA 
CGCCAGTACG ACTGTTACCT CAACAAGGAA ATGTCCATCC AGGAATTTGC CGCCCTGGTC 
TCTGGGAGGC TGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

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ppl.L cDNA ORF clone, Xenopus laevis(African clawed frog)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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