ndel1.L cDNA ORF clone, Xenopus laevis(African clawed frog)

Gene
Clones
gRNAs

Summary

Gene Symbol	ndel1.L
Entrez Gene ID	444761
Full Name	nudE neurodevelopment protein 1 like 1 L homeolog
Synonyms	eopa,mitap1,ndel1,ndel1-a,ndel1-b,nudel
General protein information	Preferred Names nudE neurodevelopment protein 1 like 1 L homeolog Names nuclear distribution protein nudE-like 1-A nudE neurodevelopment protein 1-like 1 nudE nuclear distribution E homolog-like 1 nudE nuclear distribution gene E homolog like 1
Gene Type	protein-coding
Organism	Xenopus laevis(African clawed frog)

mRNA and Protein(s)

mRNA	Protein	Name
XM_018234401.1	XP_018089890.1	nuclear distribution protein nudE-like 1-A isoform X2
XM_018234400.1	XP_018089889.1	nuclear distribution protein nudE-like 1-A isoform X1
NM_001092863.1	NP_001086332.1	nuclear distribution protein nudE-like 1-A
XM_018234402.1	XP_018089891.1	nuclear distribution protein nudE-like 1-A isoform X3

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Nudel/NudE and Lis1 promote dynein and dynactin interaction in the context of spindle morphogenesis.
Wang S, Ketcham SA, Schön A, Goodman B, Wang Y, Yates J 3rd, Freire E, Schroer TA, Zheng Y
Molecular biology of the cell24(22)3522-33(2013 Nov)

The N-terminal coiled-coil of Ndel1 is a regulated scaffold that recruits LIS1 to dynein.
Zyłkiewicz E, Kijańska M, Choi WC, Derewenda U, Derewenda ZS, Stukenberg PT
The Journal of cell biology192(3)433-45(2011 Feb)

Identification of a novel dynein binding domain in nudel essential for spindle pole organization in Xenopus egg extract.
Wang S, Zheng Y
The Journal of biological chemistry286(1)587-93(2011 Jan)

Requirement for Nudel and dynein for assembly of the lamin B spindle matrix.
Ma L, Tsai MY, Wang S, Lu B, Chen R, Yates JR III, Zhu X, Zheng Y
Nature cell biology11(3)247-56(2009 03)

Genetic and genomic tools for Xenopus research: The NIH Xenopus initiative.
Klein SL, Strausberg RL, Wagner L, Pontius J, Clifton SW, Richardson P
Developmental dynamics : an official publication of the American Association of Anatomists225(4)384-91(2002 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following ndel1.L gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ndel1.L cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OAf01976	NM_001092863.1 Latest version!	Xenopus laevis nudE neurodevelopment protein 1 like 1 L homeolog (ndel1.L), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00
OAf45196	XM_018234400.1 Latest version!	Xenopus laevis nudE neurodevelopment protein 1 like 1 L homeolog (ndel1.L), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00
OAf45197	XM_018234401.1 Latest version!	Xenopus laevis nudE neurodevelopment protein 1 like 1 L homeolog (ndel1.L), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00
OAf45198	XM_018234402.1 Latest version!	Xenopus laevis nudE neurodevelopment protein 1 like 1 L homeolog (ndel1.L), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

ndel1.L ( NM_001092863.1 ) cDNA ORF clone, Xenopus laevis(African clawed frog) -> NP_001086332.1 Xenopus laevis nudE neurodevelopment protein 1 like 1 L homeolog (ndel1.L), mRNA.

Price & Availability↑

CloneID	OAf01976
Clone ID Related Accession (Same CDS sequence)	NM_001092863.1
Accession Version	NM_001092863.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1038bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1547827200000
Organism	Xenopus laevis(African clawed frog)
Product	nuclear distribution protein nudE-like 1-A
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC074498.1. ##Evidence-Data-START## Transcript exon combination :: BC074498.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMD00012418, SAMD00012419 [ECO:0000350] ##Evidence-Data-END##

ATGGAAAACG AGGAAATCCC TGAGTTCCTG AGCCCCAAAG AGGAGATTGT CTATTGGCGG 
GAGCTTTCAA AGAGACTGAA ACAGAGTTAC CAAGAGGCAC GTGATGAACT GATAGAGTTT 
CAGGAGGGGA GCCGAGAGCT GGAGGCCGAG CTGGAAACAC AACTGGTTCA AGCGGAACAG 
AGGAACAGGG ATTTGTTAAG TGATAACCAA AGGCTAAAGT GCGAGGTGGA GTCTCTAAAG 
GAGAAGTTGG AGCACCAGTA TGCACAGAGT TACAAACAGG TGTCGCTGCT GGAGGATGAA 
TTGGCTCGTG CCCGGAGCAT TAAGGATCAG TTGCACAAGT ACGTCAGAGA GTTGGAACAG 
GCTAATGATG ACTTGGAAAG AGCCAAAAGG GCGACCATCG TATCTCTGGA AGACTTCGAA 
CAAAGGCTGA ATCAGGCAAT AGAAAGGAAC GCTTTCTTGG AGAGCGAGCT GGATGAAAAG 
GAGTCTTTGT TGGTCTCCGT GCAGAGATTG AAAGATGAGG CGCGAGACTT AAGGCAAGAA 
TTAGCAGTAC GTGAGAGACA GACGGATGGT ACAAGGAAAT CGGCCCCCAG TTCACCAACT 
CTGGACTGTG ATAAAACAGA CTCTGCAGTC CAGGCTTCTC TCTCTCTCCC AGCAACCCCT 
GTGGGCAAAA TATGTGATAA CAGCTTCACT TCACCCAAAG GGATACCAAA CGGTTTTGGA 
ACAACTCCAC TTACACCATC TGCCAGAATA TCAGCACTTA ATATTGTAGG GGACCTGTTG 
AGGAAAGTTG GGGCCTTGGA ATCTAAGCTT GCGGCTTGTA GAAACTTTGC CAAGGACCAG 
GCCTCTCGGA AATCCTACAC ACCTGTCAAC TTGAACAGCA ACAGTAGCAG CAGTGTATTA 
AACAGCAGTG GGGTAAAATA CTCTCACGCG GGGCACACGT CATTCTTTGA CAAGGGGGCA 
GTAAATGGTT ATGAACCCCC AGGAGTTTTA GGATCCCGGC CTCCGTCCCC CCCGGGAATG 
CTTCCGCTTA GTGTATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001086332.1
CDS	49..1086
Translation	MENEEIPEFLSPKEEIVYWRELSKRLKQSYQEARDELIEFQEGSRELEAELETQLVQAEQRNRDLLSDNQRLKCEVESLKEKLEHQYAQSYKQVSLLEDELARARSIKDQLHKYVRELEQANDDLERAKRATIVSLEDFEQRLNQAIERNAFLESELDEKESLLVSVQRLKDEARDLRQELAVRERQTDGTRKSAPSSPTLDCDKTDSAVQASLSLPATPVGKICDNSFTSPKGIPNGFGTTPLTPSARISALNIVGDLLRKVGALESKLAACRNFAKDQASRKSYTPVNLNSNSSSSVLNSSGVKYSHAGHTSFFDKGAVNGYEPPGVLGSRPPSPPGMLPLSV

Target ORF information:

RefSeq Version	NM_001092863.1
Organism	Xenopus laevis(African clawed frog)
Definition	Xenopus laevis nudE neurodevelopment protein 1 like 1 L homeolog (ndel1.L), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001092863.1

ORF Insert Sequence:

ATGGAAAACG AGGAAATCCC TGAGTTCCTG AGCCCCAAAG AGGAGATTGT CTATTGGCGG 
GAGCTTTCAA AGAGACTGAA ACAGAGTTAC CAAGAGGCAC GTGATGAACT GATAGAGTTT 
CAGGAGGGGA GCCGAGAGCT GGAGGCCGAG CTGGAAACAC AACTGGTTCA AGCGGAACAG 
AGGAACAGGG ATTTGTTAAG TGATAACCAA AGGCTAAAGT GCGAGGTGGA GTCTCTAAAG 
GAGAAGTTGG AGCACCAGTA TGCACAGAGT TACAAACAGG TGTCGCTGCT GGAGGATGAA 
TTGGCTCGTG CCCGGAGCAT TAAGGATCAG TTGCACAAGT ACGTCAGAGA GTTGGAACAG 
GCTAATGATG ACTTGGAAAG AGCCAAAAGG GCGACCATCG TATCTCTGGA AGACTTCGAA 
CAAAGGCTGA ATCAGGCAAT AGAAAGGAAC GCTTTCTTGG AGAGCGAGCT GGATGAAAAG 
GAGTCTTTGT TGGTCTCCGT GCAGAGATTG AAAGATGAGG CGCGAGACTT AAGGCAAGAA 
TTAGCAGTAC GTGAGAGACA GACGGATGGT ACAAGGAAAT CGGCCCCCAG TTCACCAACT 
CTGGACTGTG ATAAAACAGA CTCTGCAGTC CAGGCTTCTC TCTCTCTCCC AGCAACCCCT 
GTGGGCAAAA TATGTGATAA CAGCTTCACT TCACCCAAAG GGATACCAAA CGGTTTTGGA 
ACAACTCCAC TTACACCATC TGCCAGAATA TCAGCACTTA ATATTGTAGG GGACCTGTTG 
AGGAAAGTTG GGGCCTTGGA ATCTAAGCTT GCGGCTTGTA GAAACTTTGC CAAGGACCAG 
GCCTCTCGGA AATCCTACAC ACCTGTCAAC TTGAACAGCA ACAGTAGCAG CAGTGTATTA 
AACAGCAGTG GGGTAAAATA CTCTCACGCG GGGCACACGT CATTCTTTGA CAAGGGGGCA 
GTAAATGGTT ATGAACCCCC AGGAGTTTTA GGATCCCGGC CTCCGTCCCC CCCGGGAATG 
CTTCCGCTTA GTGTATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ndel1.L ( XM_018234400.1 ) cDNA ORF clone, Xenopus laevis(African clawed frog) -> XP_018089889.1 Xenopus laevis nudE neurodevelopment protein 1 like 1 L homeolog (ndel1.L), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OAf45196
Clone ID Related Accession (Same CDS sequence)	XM_018234400.1
Accession Version	XM_018234400.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1053bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1474214400000
Organism	Xenopus laevis(African clawed frog)
Product	nuclear distribution protein nudE-like 1-A isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030740.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus laevis Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATTTCCT TAACCATGGA AAACGAGGAA ATCCCTGAGT TCCTGAGCCC CAAAGAGGAG 
ATTGTCTATT GGCGGGAGCT TTCAAAGAGA CTGAAACAGA GTTACCAAGA GGCACGTGAT 
GAACTGATAG AGTTTCAGGA GGGGAGCCGA GAGCTGGAGG CCGAGCTGGA AACACAACTG 
GTTCAAGCGG AACAGAGGAA CAGGGATTTG TTAAGTGATA ACCAAAGGCT AAAGTGCGAG 
GTGGAGTCTC TAAAGGAGAA GTTGGAGCAC CAGTATGCAC AGAGTTACAA ACAGGTGTCG 
CTGCTGGAGG ATGAATTGGC TCGTGCCCGG AGCATTAAGG ATCAGTTGCA CAAGTACGTC 
AGAGAGTTGG AACAGGCTAA TGATGACTTG GAAAGAGCCA AAAGGGCGAC CATCGTATCT 
CTGGAAGACT TCGAACAAAG GCTGAATCAG GCAATAGAAA GGAACGCTTT CTTGGAGAGC 
GAGCTGGATG AAAAGGAGTC TTTGTTGGTC TCCGTGCAGA GATTGAAAGA TGAGGCGCGA 
GACTTAAGGC AAGAATTAGC AGTACGTGAG AGACAGACGG ATGGTACAAG GAAATCGGCC 
CCCAGTTCAC CAACTCTGGA CTGTGATAAA ACAGACTCTG CAGTCCAGGC TTCTCTCTCT 
CTCCCAGCAA CCCCTGTGGG CAAAATATGT GATAACAGCT TCACTTCACC CAAAGGGATA 
CCAAACGGTT TTGGAACAAC TCCACTTACA CCATCTGCCA GAATATCAGC ACTTAATATT 
GTAGGGGACC TGTTGAGGAA AGTTGGGGCC TTGGAATCTA AGCTTGCGGC TTGTAGAAAC 
TTTGCCAAGG ACCAGGCCTC TCGGAAATCC TACACACCTG TCAACTTGAA CAGCAACAGT 
AGCAGCAGTG TATTAAACAG CAGTGGGGTA AAATACTCTC ACGCGGGGCA CACGTCATTC 
TTTGACAAGG GGGCAGTAAA TGGTTATGAA CCCCCAGGAG TTTTAGGATC CCGGCCTCCG 
TCCCCCCCAG GAATGCTTCC GCTTAGTGTA TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_018089889.1
CDS	340..1392
Translation	MISLTMENEEIPEFLSPKEEIVYWRELSKRLKQSYQEARDELIEFQEGSRELEAELETQLVQAEQRNRDLLSDNQRLKCEVESLKEKLEHQYAQSYKQVSLLEDELARARSIKDQLHKYVRELEQANDDLERAKRATIVSLEDFEQRLNQAIERNAFLESELDEKESLLVSVQRLKDEARDLRQELAVRERQTDGTRKSAPSSPTLDCDKTDSAVQASLSLPATPVGKICDNSFTSPKGIPNGFGTTPLTPSARISALNIVGDLLRKVGALESKLAACRNFAKDQASRKSYTPVNLNSNSSSSVLNSSGVKYSHAGHTSFFDKGAVNGYEPPGVLGSRPPSPPGMLPLSV

Target ORF information:

RefSeq Version	XM_018234400.1
Organism	Xenopus laevis(African clawed frog)
Definition	Xenopus laevis nudE neurodevelopment protein 1 like 1 L homeolog (ndel1.L), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_018234400.1

ORF Insert Sequence:

ATGATTTCCT TAACCATGGA AAACGAGGAA ATCCCTGAGT TCCTGAGCCC CAAAGAGGAG 
ATTGTCTATT GGCGGGAGCT TTCAAAGAGA CTGAAACAGA GTTACCAAGA GGCACGTGAT 
GAACTGATAG AGTTTCAGGA GGGGAGCCGA GAGCTGGAGG CCGAGCTGGA AACACAACTG 
GTTCAAGCGG AACAGAGGAA CAGGGATTTG TTAAGTGATA ACCAAAGGCT AAAGTGCGAG 
GTGGAGTCTC TAAAGGAGAA GTTGGAGCAC CAGTATGCAC AGAGTTACAA ACAGGTGTCG 
CTGCTGGAGG ATGAATTGGC TCGTGCCCGG AGCATTAAGG ATCAGTTGCA CAAGTACGTC 
AGAGAGTTGG AACAGGCTAA TGATGACTTG GAAAGAGCCA AAAGGGCGAC CATCGTATCT 
CTGGAAGACT TCGAACAAAG GCTGAATCAG GCAATAGAAA GGAACGCTTT CTTGGAGAGC 
GAGCTGGATG AAAAGGAGTC TTTGTTGGTC TCCGTGCAGA GATTGAAAGA TGAGGCGCGA 
GACTTAAGGC AAGAATTAGC AGTACGTGAG AGACAGACGG ATGGTACAAG GAAATCGGCC 
CCCAGTTCAC CAACTCTGGA CTGTGATAAA ACAGACTCTG CAGTCCAGGC TTCTCTCTCT 
CTCCCAGCAA CCCCTGTGGG CAAAATATGT GATAACAGCT TCACTTCACC CAAAGGGATA 
CCAAACGGTT TTGGAACAAC TCCACTTACA CCATCTGCCA GAATATCAGC ACTTAATATT 
GTAGGGGACC TGTTGAGGAA AGTTGGGGCC TTGGAATCTA AGCTTGCGGC TTGTAGAAAC 
TTTGCCAAGG ACCAGGCCTC TCGGAAATCC TACACACCTG TCAACTTGAA CAGCAACAGT 
AGCAGCAGTG TATTAAACAG CAGTGGGGTA AAATACTCTC ACGCGGGGCA CACGTCATTC 
TTTGACAAGG GGGCAGTAAA TGGTTATGAA CCCCCAGGAG TTTTAGGATC CCGGCCTCCG 
TCCCCCCCAG GAATGCTTCC GCTTAGTGTA TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ndel1.L ( XM_018234401.1 ) cDNA ORF clone, Xenopus laevis(African clawed frog) -> XP_018089890.1 Xenopus laevis nudE neurodevelopment protein 1 like 1 L homeolog (ndel1.L), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OAf45197
Clone ID Related Accession (Same CDS sequence)	XM_018234401.1
Accession Version	XM_018234401.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1038bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1474214400000
Organism	Xenopus laevis(African clawed frog)
Product	nuclear distribution protein nudE-like 1-A isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030740.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus laevis Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAAACG AGGAAATCCC TGAGTTCCTG AGCCCCAAAG AGGAGATTGT CTATTGGCGG 
GAGCTTTCAA AGAGACTGAA ACAGAGTTAC CAAGAGGCAC GTGATGAACT GATAGAGTTT 
CAGGAGGGGA GCCGAGAGCT GGAGGCCGAG CTGGAAACAC AACTGGTTCA AGCGGAACAG 
AGGAACAGGG ATTTGTTAAG TGATAACCAA AGGCTAAAGT GCGAGGTGGA GTCTCTAAAG 
GAGAAGTTGG AGCACCAGTA TGCACAGAGT TACAAACAGG TGTCGCTGCT GGAGGATGAA 
TTGGCTCGTG CCCGGAGCAT TAAGGATCAG TTGCACAAGT ACGTCAGAGA GTTGGAACAG 
GCTAATGATG ACTTGGAAAG AGCCAAAAGG GCGACCATCG TATCTCTGGA AGACTTCGAA 
CAAAGGCTGA ATCAGGCAAT AGAAAGGAAC GCTTTCTTGG AGAGCGAGCT GGATGAAAAG 
GAGTCTTTGT TGGTCTCCGT GCAGAGATTG AAAGATGAGG CGCGAGACTT AAGGCAAGAA 
TTAGCAGTAC GTGAGAGACA GACGGATGGT ACAAGGAAAT CGGCCCCCAG TTCACCAACT 
CTGGACTGTG ATAAAACAGA CTCTGCAGTC CAGGCTTCTC TCTCTCTCCC AGCAACCCCT 
GTGGGCAAAA TATGTGATAA CAGCTTCACT TCACCCAAAG GGATACCAAA CGGTTTTGGA 
ACAACTCCAC TTACACCATC TGCCAGAATA TCAGCACTTA ATATTGTAGG GGACCTGTTG 
AGGAAAGTTG GGGCCTTGGA ATCTAAGCTT GCGGCTTGTA GAAACTTTGC CAAGGACCAG 
GCCTCTCGGA AATCCTACAC ACCTGTCAAC TTGAACAGCA ACAGTAGCAG CAGTGTATTA 
AACAGCAGTG GGGTAAAATA CTCTCACGCG GGGCACACGT CATTCTTTGA CAAGGGGGCA 
GTAAATGGTT ATGAACCCCC AGGAGTTTTA GGATCCCGGC CTCCGTCCCC CCCAGGAATG 
CTTCCGCTTA GTGTATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_018089890.1
CDS	120..1157
Translation	MENEEIPEFLSPKEEIVYWRELSKRLKQSYQEARDELIEFQEGSRELEAELETQLVQAEQRNRDLLSDNQRLKCEVESLKEKLEHQYAQSYKQVSLLEDELARARSIKDQLHKYVRELEQANDDLERAKRATIVSLEDFEQRLNQAIERNAFLESELDEKESLLVSVQRLKDEARDLRQELAVRERQTDGTRKSAPSSPTLDCDKTDSAVQASLSLPATPVGKICDNSFTSPKGIPNGFGTTPLTPSARISALNIVGDLLRKVGALESKLAACRNFAKDQASRKSYTPVNLNSNSSSSVLNSSGVKYSHAGHTSFFDKGAVNGYEPPGVLGSRPPSPPGMLPLSV

Target ORF information:

RefSeq Version	XM_018234401.1
Organism	Xenopus laevis(African clawed frog)
Definition	Xenopus laevis nudE neurodevelopment protein 1 like 1 L homeolog (ndel1.L), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_018234401.1

ORF Insert Sequence:

ATGGAAAACG AGGAAATCCC TGAGTTCCTG AGCCCCAAAG AGGAGATTGT CTATTGGCGG 
GAGCTTTCAA AGAGACTGAA ACAGAGTTAC CAAGAGGCAC GTGATGAACT GATAGAGTTT 
CAGGAGGGGA GCCGAGAGCT GGAGGCCGAG CTGGAAACAC AACTGGTTCA AGCGGAACAG 
AGGAACAGGG ATTTGTTAAG TGATAACCAA AGGCTAAAGT GCGAGGTGGA GTCTCTAAAG 
GAGAAGTTGG AGCACCAGTA TGCACAGAGT TACAAACAGG TGTCGCTGCT GGAGGATGAA 
TTGGCTCGTG CCCGGAGCAT TAAGGATCAG TTGCACAAGT ACGTCAGAGA GTTGGAACAG 
GCTAATGATG ACTTGGAAAG AGCCAAAAGG GCGACCATCG TATCTCTGGA AGACTTCGAA 
CAAAGGCTGA ATCAGGCAAT AGAAAGGAAC GCTTTCTTGG AGAGCGAGCT GGATGAAAAG 
GAGTCTTTGT TGGTCTCCGT GCAGAGATTG AAAGATGAGG CGCGAGACTT AAGGCAAGAA 
TTAGCAGTAC GTGAGAGACA GACGGATGGT ACAAGGAAAT CGGCCCCCAG TTCACCAACT 
CTGGACTGTG ATAAAACAGA CTCTGCAGTC CAGGCTTCTC TCTCTCTCCC AGCAACCCCT 
GTGGGCAAAA TATGTGATAA CAGCTTCACT TCACCCAAAG GGATACCAAA CGGTTTTGGA 
ACAACTCCAC TTACACCATC TGCCAGAATA TCAGCACTTA ATATTGTAGG GGACCTGTTG 
AGGAAAGTTG GGGCCTTGGA ATCTAAGCTT GCGGCTTGTA GAAACTTTGC CAAGGACCAG 
GCCTCTCGGA AATCCTACAC ACCTGTCAAC TTGAACAGCA ACAGTAGCAG CAGTGTATTA 
AACAGCAGTG GGGTAAAATA CTCTCACGCG GGGCACACGT CATTCTTTGA CAAGGGGGCA 
GTAAATGGTT ATGAACCCCC AGGAGTTTTA GGATCCCGGC CTCCGTCCCC CCCAGGAATG 
CTTCCGCTTA GTGTATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ndel1.L ( XM_018234402.1 ) cDNA ORF clone, Xenopus laevis(African clawed frog) -> XP_018089891.1 Xenopus laevis nudE neurodevelopment protein 1 like 1 L homeolog (ndel1.L), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OAf45198
Clone ID Related Accession (Same CDS sequence)	XM_018234402.1
Accession Version	XM_018234402.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1014bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1474214400000
Organism	Xenopus laevis(African clawed frog)
Product	nuclear distribution protein nudE-like 1-A isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030740.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus laevis Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATTTCCT TAACCATGGA AAACGAGGAA ATCCCTGAGT TCCTGAGCCC CAAAGAGGAG 
ATTGTCTATT GGCGGGAGCT TTCAAAGAGA CTGAAACAGA GTTACCAAGA GGCACGTGAT 
GAACTGATAG AGTTTCAGGA GGGGAGCCGA GAGCTGGAGG CCGAGCTGGA AACACAACTG 
GTTCAAGCGG AACAGAGGAA CAGGGATTTG TTAAGTGATA ACCAAAGGCT AAAGTGCGAG 
GTGGAGTCTC TAAAGGAGAA GTTGGAGCAC CAGTATGCAC AGAGTTACAA ACAGGTGTCG 
CTGCTGGAGG ATGAATTGGC TCGTGCCCGG AGCATTAAGG ATCAGTTGCA CAAGTACGTC 
AGAGAGTTGG AACAGGCTAA TGATGACTTG GAAAGAGCCA AAAGGGCGAC CATCGTATCT 
CTGGAAGACT TCGAACAAAG GCTGAATCAG GCAATAGAAA GGAACGCTTT CTTGGAGAGC 
GAGCTGGATG AAAAGGAGTC TTTGTTGGTC TCCGTGCAGA GATTGAAAGA TGAGGCGCGA 
GACTTAAGGC AAGAATTAGC AGTACGTGAG AGACAGACGG ATGGTACAAG GAAATCGGCC 
CCCAGTTCAC CAACTCTGGA CTGTGATAAA ACAGACTCTG CAGTCCAGGC TTCTCTCTCT 
CTCCCAGCAA CCCCTGTGGG CAAAATATGT GATAACAGCT TCACTTCACC CAAAGGGATA 
CCAAACGGTT TTGGAACAAC TCCACTTACA CCATCTGCCA GAATATCAGC ACTTAATATT 
GTAGGGGACC TGTTGAGGAA AGTTGGGGCC TTGGAATCTA AGCTTGCGGC TTGTAGAAAC 
TTTGCCAAGG ACCAGGCCTC TCGGAAATCC TACACACCTG TCAACTTGAA CAGCAACAGT 
AGCAGCAGTG TATTAAACAG CAGTGGGGTA AAATACTCTC ACGCGGGGCA CACGTCATTC 
TTTGACAAGG GGCAGGAGAA GGTCATTTTC CCCACGTTGT TTATGGGGCA GTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_018089891.1
CDS	346..1359
Translation	MISLTMENEEIPEFLSPKEEIVYWRELSKRLKQSYQEARDELIEFQEGSRELEAELETQLVQAEQRNRDLLSDNQRLKCEVESLKEKLEHQYAQSYKQVSLLEDELARARSIKDQLHKYVRELEQANDDLERAKRATIVSLEDFEQRLNQAIERNAFLESELDEKESLLVSVQRLKDEARDLRQELAVRERQTDGTRKSAPSSPTLDCDKTDSAVQASLSLPATPVGKICDNSFTSPKGIPNGFGTTPLTPSARISALNIVGDLLRKVGALESKLAACRNFAKDQASRKSYTPVNLNSNSSSSVLNSSGVKYSHAGHTSFFDKGQEKVIFPTLFMGQ

Target ORF information:

RefSeq Version	XM_018234402.1
Organism	Xenopus laevis(African clawed frog)
Definition	Xenopus laevis nudE neurodevelopment protein 1 like 1 L homeolog (ndel1.L), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_018234402.1

ORF Insert Sequence:

ATGATTTCCT TAACCATGGA AAACGAGGAA ATCCCTGAGT TCCTGAGCCC CAAAGAGGAG 
ATTGTCTATT GGCGGGAGCT TTCAAAGAGA CTGAAACAGA GTTACCAAGA GGCACGTGAT 
GAACTGATAG AGTTTCAGGA GGGGAGCCGA GAGCTGGAGG CCGAGCTGGA AACACAACTG 
GTTCAAGCGG AACAGAGGAA CAGGGATTTG TTAAGTGATA ACCAAAGGCT AAAGTGCGAG 
GTGGAGTCTC TAAAGGAGAA GTTGGAGCAC CAGTATGCAC AGAGTTACAA ACAGGTGTCG 
CTGCTGGAGG ATGAATTGGC TCGTGCCCGG AGCATTAAGG ATCAGTTGCA CAAGTACGTC 
AGAGAGTTGG AACAGGCTAA TGATGACTTG GAAAGAGCCA AAAGGGCGAC CATCGTATCT 
CTGGAAGACT TCGAACAAAG GCTGAATCAG GCAATAGAAA GGAACGCTTT CTTGGAGAGC 
GAGCTGGATG AAAAGGAGTC TTTGTTGGTC TCCGTGCAGA GATTGAAAGA TGAGGCGCGA 
GACTTAAGGC AAGAATTAGC AGTACGTGAG AGACAGACGG ATGGTACAAG GAAATCGGCC 
CCCAGTTCAC CAACTCTGGA CTGTGATAAA ACAGACTCTG CAGTCCAGGC TTCTCTCTCT 
CTCCCAGCAA CCCCTGTGGG CAAAATATGT GATAACAGCT TCACTTCACC CAAAGGGATA 
CCAAACGGTT TTGGAACAAC TCCACTTACA CCATCTGCCA GAATATCAGC ACTTAATATT 
GTAGGGGACC TGTTGAGGAA AGTTGGGGCC TTGGAATCTA AGCTTGCGGC TTGTAGAAAC 
TTTGCCAAGG ACCAGGCCTC TCGGAAATCC TACACACCTG TCAACTTGAA CAGCAACAGT 
AGCAGCAGTG TATTAAACAG CAGTGGGGTA AAATACTCTC ACGCGGGGCA CACGTCATTC 
TTTGACAAGG GGCAGGAGAA GGTCATTTTC CCCACGTTGT TTATGGGGCA GTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Nudel/NudE and Lis1 promote dynein and dynactin interaction in the context of spindle morphogenesis.
Molecular biology of the cell24(22)3522-33(2013 Nov)
Wang S,Ketcham SA,Schön A,Goodman B,Wang Y,Yates J 3rd,Freire E,Schroer TA,Zheng Y

The N-terminal coiled-coil of Ndel1 is a regulated scaffold that recruits LIS1 to dynein.
The Journal of cell biology192(3)433-45(2011 Feb)
Zyłkiewicz E,Kijańska M,Choi WC,Derewenda U,Derewenda ZS,Stukenberg PT

Identification of a novel dynein binding domain in nudel essential for spindle pole organization in Xenopus egg extract.
The Journal of biological chemistry286(1)587-93(2011 Jan)
Wang S,Zheng Y

Requirement for Nudel and dynein for assembly of the lamin B spindle matrix.
Nature cell biology11(3)247-56(2009 03)
Ma L,Tsai MY,Wang S,Lu B,Chen R,Yates JR III,Zhu X,Zheng Y

Genetic and genomic tools for Xenopus research: The NIH Xenopus initiative.
Developmental dynamics : an official publication of the American Association of Anatomists225(4)384-91(2002 Dec)
Klein SL,Strausberg RL,Wagner L,Pontius J,Clifton SW,Richardson P

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ndel1.L cDNA ORF clone, Xenopus laevis(African clawed frog)

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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