mixer.S cDNA ORF clone, Xenopus laevis(African clawed frog)

Gene
Clones
gRNAs

Summary

Gene Symbol	mixer.S
Entrez Gene ID	398035
Full Name	mix-like endodermal regulator L homeolog
Synonyms	mixer,mixer.S,tMixer
General protein information	Preferred Names mix-like endodermal regulator L homeolog Names mix-like endodermal regulator L homeolog mix-like endodermal regulator S homeolog homeodomain protein Mix.3
Gene Type	protein-coding
Organism	Xenopus laevis(African clawed frog)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001088291.1	NP_001081760.1	mix-like endodermal regulator L homeolog
XM_018261711.1	XP_018117200.1	mix-like endodermal regulator S homeolog isoform X1

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

CDK9/cyclin complexes modulate endoderm induction by direct interaction with Mix.3/mixer.
Zhu H, Doherty JR, Kuliyev E, Mead PE
Developmental dynamics : an official publication of the American Association of Anatomists238(6)1346-57(2009 Jun)

Determination of the minimal domains of Mix.3/Mixer required for endoderm development.
Doherty JR, Zhu H, Kuliyev E, Mead PE
Mechanisms of development123(1)56-66(2006 Jan)

Genetic and genomic tools for Xenopus research: The NIH Xenopus initiative.
Klein SL, Strausberg RL, Wagner L, Pontius J, Clifton SW, Richardson P
Developmental dynamics : an official publication of the American Association of Anatomists225(4)384-91(2002 Dec)

Cloning of Mix-related homeodomain proteins using fast retrieval of gel shift activities, (FROGS), a technique for the isolation of DNA-binding proteins.
Mead PE, Zhou Y, Lustig KD, Huber TL, Kirschner MW, Zon LI
Proceedings of the National Academy of Sciences of the United States of America95(19)11251-6(1998 Sep)

Mixer, a homeobox gene required for endoderm development.
Henry GL, Melton DA
Science (New York, N.Y.)281(5373)91-6(1998 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following mixer.S gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the mixer.S cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OAf10561	NM_001088291.1 Latest version!	Xenopus laevis mix-like endodermal regulator L homeolog (mixer.L), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00
OAf31335	XM_018261711.1 Latest version!	Xenopus laevis mix-like endodermal regulator S homeolog (mixer.S), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

mixer.S ( NM_001088291.1 ) cDNA ORF clone, Xenopus laevis(African clawed frog) -> NP_001081760.1 Xenopus laevis mix-like endodermal regulator L homeolog (mixer.L), mRNA.

Price & Availability↑

CloneID	OAf10561
Clone ID Related Accession (Same CDS sequence)	NM_001088291.1
Accession Version	NM_001088291.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1116bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1537286400000
Organism	Xenopus laevis(African clawed frog)
Product	mix-like endodermal regulator L homeolog
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AF081350.1. On Jul 25, 2007 this sequence version replaced NM_001088292.1. ##Evidence-Data-START## Transcript exon combination :: AF081350.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMD00012418, SAMD00012419 [ECO:0000350] ##Evidence-Data-END##

ATGGACACGT TCAGCCAACA ACTGGAGGAC TTCTACCCAT CTTGCTTCTC TGCCAGGTCC 
AGCCCAGTGG GCTTCACTGA TCCACCAGCC CAGCACTTAA CCATGAGCCT TGGTGCCATT 
CAGAAGGATT TCCAAGAGTC CAGCTTAAAG GCAAACGTCC AGCCAGTCAG TGATCCTCAA 
ACACTAGGGA CCCAGAAGTC CACCCCTCCT ACAAAACAAG AAATGGTCTC CCCGGTATCT 
TCAGTTGATG TGACATTGGG ATCACAACGC CGCAAGAGAA CATTTTACAG CCAGAACAAG 
CTGGATGTTC TAGAACAGTT CTTCCAGACC AACATGTATC CAGACATTCA CCACCGGGAA 
GAACTGGCTA AACGGATTTA CATCCCAGAG TCCAGAGTTC AGGTCTGGTT CCAGAACAGA 
AGAGCAAAGG AGCGCCGCGA TAAAGCCAAA TTAAACCCCT CGCCAGCAGT AGGCGTGTGC 
TACCCCAGTC TTCGACAACC CAATAAAAAA ATGTACCCCT CCAACAACCC AACACCAAAT 
GTGCCTGTTT CCCAGCAACA CATGGTTTTC CAAAAACCAC AGGGTCAACT GTTCATGAAT 
TCCCAGCAGA ATCCGTTCCA GCCAACTCAG GAATCTCAGC TTTGTTCAGA ATCCATCTAC 
GCTGTTTCCC AGCAGAGGAT CCTGATGCAG CAGGCTGCAC GGAGCAGCTA CCATGGAATT 
TCAGCATCTT ATAAACCTAC AGACACTCAG CAGCACTTCT ACTCATACAT GAGCCCAATG 
GGAGGCACCC AGGAGAAAGT CATGGATCTA AGCAAGAAGC ACAGTCAGAT GCCCTTCCAT 
CCCAGTCTTC TAATGGACTT CAACAACTTC CCTCCCAACA AGACCATCAC TCCAGATATG 
AATGTTAGAA TCCCACCAAT TCCTGTCTCT TCACCGTCAA ACAACCACAG TTGGATGAAT 
GTCTTTAATA CCAAAGAGGC CGGCCCATTG GTGTCCTTGC CAGAGGATGT CTATGAGGAA 
TTCTCTCCGG TCTCTGATTC TGGTGTTAGT GATGGATCTA CCATGTCTTT GACAGACTTT 
AAAGATAATG ATGGATCTGT GCTTGACAAC CTGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001081760.1
CDS	64..1179
Translation	MDTFSQQLEDFYPSCFSARSSPVGFTDPPAQHLTMSLGAIQKDFQESSLKANVQPVSDPQTLGTQKSTPPTKQEMVSPVSSVDVTLGSQRRKRTFYSQNKLDVLEQFFQTNMYPDIHHREELAKRIYIPESRVQVWFQNRRAKERRDKAKLNPSPAVGVCYPSLRQPNKKMYPSNNPTPNVPVSQQHMVFQKPQGQLFMNSQQNPFQPTQESQLCSESIYAVSQQRILMQQAARSSYHGISASYKPTDTQQHFYSYMSPMGGTQEKVMDLSKKHSQMPFHPSLLMDFNNFPPNKTITPDMNVRIPPIPVSSPSNNHSWMNVFNTKEAGPLVSLPEDVYEEFSPVSDSGVSDGSTMSLTDFKDNDGSVLDNL

Target ORF information:

RefSeq Version	NM_001088291.1
Organism	Xenopus laevis(African clawed frog)
Definition	Xenopus laevis mix-like endodermal regulator L homeolog (mixer.L), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001088291.1

ORF Insert Sequence:

ATGGACACGT TCAGCCAACA ACTGGAGGAC TTCTACCCAT CTTGCTTCTC TGCCAGGTCC 
AGCCCAGTGG GCTTCACTGA TCCACCAGCC CAGCACTTAA CCATGAGCCT TGGTGCCATT 
CAGAAGGATT TCCAAGAGTC CAGCTTAAAG GCAAACGTCC AGCCAGTCAG TGATCCTCAA 
ACACTAGGGA CCCAGAAGTC CACCCCTCCT ACAAAACAAG AAATGGTCTC CCCGGTATCT 
TCAGTTGATG TGACATTGGG ATCACAACGC CGCAAGAGAA CATTTTACAG CCAGAACAAG 
CTGGATGTTC TAGAACAGTT CTTCCAGACC AACATGTATC CAGACATTCA CCACCGGGAA 
GAACTGGCTA AACGGATTTA CATCCCAGAG TCCAGAGTTC AGGTCTGGTT CCAGAACAGA 
AGAGCAAAGG AGCGCCGCGA TAAAGCCAAA TTAAACCCCT CGCCAGCAGT AGGCGTGTGC 
TACCCCAGTC TTCGACAACC CAATAAAAAA ATGTACCCCT CCAACAACCC AACACCAAAT 
GTGCCTGTTT CCCAGCAACA CATGGTTTTC CAAAAACCAC AGGGTCAACT GTTCATGAAT 
TCCCAGCAGA ATCCGTTCCA GCCAACTCAG GAATCTCAGC TTTGTTCAGA ATCCATCTAC 
GCTGTTTCCC AGCAGAGGAT CCTGATGCAG CAGGCTGCAC GGAGCAGCTA CCATGGAATT 
TCAGCATCTT ATAAACCTAC AGACACTCAG CAGCACTTCT ACTCATACAT GAGCCCAATG 
GGAGGCACCC AGGAGAAAGT CATGGATCTA AGCAAGAAGC ACAGTCAGAT GCCCTTCCAT 
CCCAGTCTTC TAATGGACTT CAACAACTTC CCTCCCAACA AGACCATCAC TCCAGATATG 
AATGTTAGAA TCCCACCAAT TCCTGTCTCT TCACCGTCAA ACAACCACAG TTGGATGAAT 
GTCTTTAATA CCAAAGAGGC CGGCCCATTG GTGTCCTTGC CAGAGGATGT CTATGAGGAA 
TTCTCTCCGG TCTCTGATTC TGGTGTTAGT GATGGATCTA CCATGTCTTT GACAGACTTT 
AAAGATAATG ATGGATCTGT GCTTGACAAC CTGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

mixer.S ( XM_018261711.1 ) cDNA ORF clone, Xenopus laevis(African clawed frog) -> XP_018117200.1 Xenopus laevis mix-like endodermal regulator S homeolog (mixer.S), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OAf31335
Clone ID Related Accession (Same CDS sequence)	XM_018261711.1
Accession Version	XM_018261711.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1311bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1474214400000
Organism	Xenopus laevis(African clawed frog)
Product	mix-like endodermal regulator S homeolog isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030732.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus laevis Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGGTCTA CTTGGCAAGT TGGTTCCATC CATGCGTTTC TAGACATGTT CACACCTAAT 
TGGCTAAAGG ATTCAGTGTC CCCCAGTGGT ATAAGTAGGG ACTCAGGTCT CATTCAGTCA 
TTCAACCAAC TGAGCAGCAC TGGTCACTTT GGGAGCCACA AGATTTGGGA GTTCAGGCAG 
CAGCTAGCCC CTAGGATGGA CACGTTCAGC CAACAACTGG AGGACTTCTA CCCATCTTGC 
TTCTCTGCCA GGTCCAGCCC AGTGGGCTTC ACTGATCCAC CAGCCCAGCA CTTAACCATG 
AGCCTTGGTG CCATTCAGAA GGATTTCCAA GAGTCCAGCT TGAAGGCAAA CGTCCAGCCA 
GTCAGTGATC CTCAAACACT AGGGACCCAG AAGTCCACCC CTCCTACAAA ACAAGAAATG 
GTCTCCCCGG TATCTTCAGT TGATGTGACA TTGGGATCAC AACGCCGCAA GAGAACATTT 
TACAGCCAGA ACAAGCTGGA TGTTCTAGAA CAGTTCTTCC AGACCAACAT GTATCCAGAC 
ATTCACCACC GGGAAGAACT GGCTAAACGG ATTTACATCC CAGAGTCCAG AGTTCAGGTC 
TGGTTCCAGA ACAGAAGAGC AAAGGAGCGC CGCGATAAAG CCAAATTAAA CCCCTCGCCA 
GCAGTAGGCG TGTGCTACCC CAGTCTTCGA CAACCCAATA AAGAAATGTA CCCCTCCAAC 
AACCCAACAC CAAATGTGCC TGTTTCCCAG CAACACATGG TTTTCCAAAA ACCACAGGGT 
CAACTGTTCA TGAATTCCCA GCAGAATCCG TTCCAGCCAA CTCAGGAATC TCAGCTTTGT 
TCAGAATCCA CCTACGCTGT TTCCCAGCAG AGGATCCTGA TGCAGCAGGC TGCACGGAGC 
AGCTACCATG GAATTTCAGC ATCTTATAAA CCTACAGACA CTCAGCAGCA CTTCTACTCA 
TACATGAGCC CAATGGGAGG CACCCAGGAG AAAGTCATGG ATCTAAGCAA GAAGCACAGT 
CAGATGCCCT TCCATCCCAG TCTTCTAATG GACTTCAACA ACTTCCCTCC CAACAAGACC 
ATCACTCCAG ATATGAATGT TAGAATCCCA CCAATTCCTG TCTCTGCACC GTCAAACAAC 
CACAGTCGGA TGAATGTCTT TAATACCAAA GAGGCCGGCC CATTGGTGTC CTTGCCAGAG 
GATGTCTATG AGGAATTCTC TCCGGTCTCT GATTCTGGTG TTAGTGATGG ATCTACCATG 
TCTTTGACAG ACTTTAAAGA TAATGATGGA TCTGTGCTTG ACAACCTGTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_018117200.1
CDS	16..1326
Translation	MGSTWQVGSIHAFLDMFTPNWLKDSVSPSGISRDSGLIQSFNQLSSTGHFGSHKIWEFRQQLAPRMDTFSQQLEDFYPSCFSARSSPVGFTDPPAQHLTMSLGAIQKDFQESSLKANVQPVSDPQTLGTQKSTPPTKQEMVSPVSSVDVTLGSQRRKRTFYSQNKLDVLEQFFQTNMYPDIHHREELAKRIYIPESRVQVWFQNRRAKERRDKAKLNPSPAVGVCYPSLRQPNKEMYPSNNPTPNVPVSQQHMVFQKPQGQLFMNSQQNPFQPTQESQLCSESTYAVSQQRILMQQAARSSYHGISASYKPTDTQQHFYSYMSPMGGTQEKVMDLSKKHSQMPFHPSLLMDFNNFPPNKTITPDMNVRIPPIPVSAPSNNHSRMNVFNTKEAGPLVSLPEDVYEEFSPVSDSGVSDGSTMSLTDFKDNDGSVLDNL

Target ORF information:

RefSeq Version	XM_018261711.1
Organism	Xenopus laevis(African clawed frog)
Definition	Xenopus laevis mix-like endodermal regulator S homeolog (mixer.S), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_018261711.1

ORF Insert Sequence:

ATGGGGTCTA CTTGGCAAGT TGGTTCCATC CATGCGTTTC TAGACATGTT CACACCTAAT 
TGGCTAAAGG ATTCAGTGTC CCCCAGTGGT ATAAGTAGGG ACTCAGGTCT CATTCAGTCA 
TTCAACCAAC TGAGCAGCAC TGGTCACTTT GGGAGCCACA AGATTTGGGA GTTCAGGCAG 
CAGCTAGCCC CTAGGATGGA CACGTTCAGC CAACAACTGG AGGACTTCTA CCCATCTTGC 
TTCTCTGCCA GGTCCAGCCC AGTGGGCTTC ACTGATCCAC CAGCCCAGCA CTTAACCATG 
AGCCTTGGTG CCATTCAGAA GGATTTCCAA GAGTCCAGCT TGAAGGCAAA CGTCCAGCCA 
GTCAGTGATC CTCAAACACT AGGGACCCAG AAGTCCACCC CTCCTACAAA ACAAGAAATG 
GTCTCCCCGG TATCTTCAGT TGATGTGACA TTGGGATCAC AACGCCGCAA GAGAACATTT 
TACAGCCAGA ACAAGCTGGA TGTTCTAGAA CAGTTCTTCC AGACCAACAT GTATCCAGAC 
ATTCACCACC GGGAAGAACT GGCTAAACGG ATTTACATCC CAGAGTCCAG AGTTCAGGTC 
TGGTTCCAGA ACAGAAGAGC AAAGGAGCGC CGCGATAAAG CCAAATTAAA CCCCTCGCCA 
GCAGTAGGCG TGTGCTACCC CAGTCTTCGA CAACCCAATA AAGAAATGTA CCCCTCCAAC 
AACCCAACAC CAAATGTGCC TGTTTCCCAG CAACACATGG TTTTCCAAAA ACCACAGGGT 
CAACTGTTCA TGAATTCCCA GCAGAATCCG TTCCAGCCAA CTCAGGAATC TCAGCTTTGT 
TCAGAATCCA CCTACGCTGT TTCCCAGCAG AGGATCCTGA TGCAGCAGGC TGCACGGAGC 
AGCTACCATG GAATTTCAGC ATCTTATAAA CCTACAGACA CTCAGCAGCA CTTCTACTCA 
TACATGAGCC CAATGGGAGG CACCCAGGAG AAAGTCATGG ATCTAAGCAA GAAGCACAGT 
CAGATGCCCT TCCATCCCAG TCTTCTAATG GACTTCAACA ACTTCCCTCC CAACAAGACC 
ATCACTCCAG ATATGAATGT TAGAATCCCA CCAATTCCTG TCTCTGCACC GTCAAACAAC 
CACAGTCGGA TGAATGTCTT TAATACCAAA GAGGCCGGCC CATTGGTGTC CTTGCCAGAG 
GATGTCTATG AGGAATTCTC TCCGGTCTCT GATTCTGGTG TTAGTGATGG ATCTACCATG 
TCTTTGACAG ACTTTAAAGA TAATGATGGA TCTGTGCTTG ACAACCTGTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

CDK9/cyclin complexes modulate endoderm induction by direct interaction with Mix.3/mixer.
Developmental dynamics : an official publication of the American Association of Anatomists238(6)1346-57(2009 Jun)
Zhu H,Doherty JR,Kuliyev E,Mead PE

Determination of the minimal domains of Mix.3/Mixer required for endoderm development.
Mechanisms of development123(1)56-66(2006 Jan)
Doherty JR,Zhu H,Kuliyev E,Mead PE

Genetic and genomic tools for Xenopus research: The NIH Xenopus initiative.
Developmental dynamics : an official publication of the American Association of Anatomists225(4)384-91(2002 Dec)
Klein SL,Strausberg RL,Wagner L,Pontius J,Clifton SW,Richardson P

Cloning of Mix-related homeodomain proteins using fast retrieval of gel shift activities, (FROGS), a technique for the isolation of DNA-binding proteins.
Proceedings of the National Academy of Sciences of the United States of America95(19)11251-6(1998 Sep)
Mead PE,Zhou Y,Lustig KD,Huber TL,Kirschner MW,Zon LI

Mixer, a homeobox gene required for endoderm development.
Science (New York, N.Y.)281(5373)91-6(1998 Jul)
Henry GL,Melton DA

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mixer.S cDNA ORF clone, Xenopus laevis(African clawed frog)

Gene

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gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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