col2a1.S cDNA ORF clone, Xenopus laevis(African clawed frog)

Gene
Clones
gRNAs

Summary

Gene Symbol	col2a1.S
Entrez Gene ID	397739
Full Name	collagen, type II, alpha 1 S homeolog
Synonyms	col11a3,col2a1-a,col2a1-b,col2a1b,xcol2a1
General protein information	Preferred Names collagen, type II, alpha 1 S homeolog Names collagen, type II, alpha 1 S homeolog X-col II alpha-1 type II' collagen
Gene Type	protein-coding
Organism	Xenopus laevis(African clawed frog)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001087791.1	NP_001081260.1	collagen, type II, alpha 1 S homeolog precursor

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Genetic and genomic tools for Xenopus research: The NIH Xenopus initiative.
Klein SL, Strausberg RL, Wagner L, Pontius J, Clifton SW, Richardson P
Developmental dynamics : an official publication of the American Association of Anatomists225(4)384-91(2002 Dec)

Expression of two nonallelic type II procollagen genes during Xenopus laevis embryogenesis is characterized by stage-specific production of alternatively spliced transcripts.
Su MW, Suzuki HR, Bieker JJ, Solursh M, Ramirez F
The Journal of cell biology115(2)565-75(1991 Oct)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following col2a1.S gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the col2a1.S cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OAf09995	NM_001087791.1 Latest version!	Xenopus laevis collagen, type II, alpha 1 S homeolog (col2a1.S), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

col2a1.S ( NM_001087791.1 ) cDNA ORF clone, Xenopus laevis(African clawed frog) -> NP_001081260.1 Xenopus laevis collagen, type II, alpha 1 S homeolog (col2a1.S), mRNA.

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CloneID	OAf09995
Clone ID Related Accession (Same CDS sequence)	NM_001087791.1
Accession Version	NM_001087791.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4476bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1477497600000
Organism	Xenopus laevis(African clawed frog)
Product	collagen, type II, alpha 1 S homeolog precursor
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from M63596.1. On Jul 25, 2007 this sequence version replaced NM_001087792.1. ##Evidence-Data-START## Transcript exon combination :: M63596.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMD00012418, SAMD00012419 [ECO:0000350] ##Evidence-Data-END##

ATGTTTAGCT TTGTGGATTC AAGGACTCTA GTGCTGTTCG CAGCCACACA AGTCATTTTA 
TTAGCGGTTG TACGATGCCA AGATGAAGAA GATGTCCTGG ATACAGGCAG CTGCGTGCAG 
GATGGGCAGA GGTATAGTGA TAAAGATGTG TGGAAACCCG AGCCCTGCCA AATCTGTGTC 
TGTGACACTG GGACTGTTCT CTGCGATGAA ATAATCTGCG AAGAGTCCAA AGATTGCCCC 
AATGCTGAGA TCCCCTTCGG AGAGTGCTGT CCCATCTGCC CAACTGAGCA ATCTTCTACC 
TCCAGTGGGC AAGGAGTGGC CAAGGGACAG AAAGGTGAAC CTGGTGATAT TAAAGATGTT 
GTAGGACCAA GAGGACCACC TGGACCACAG GGACCTTCTG GTGAACAAGG ACCTAGAGGA 
GAACGTGGAG ATAAGGGCGA GACTGGTGCT CCCGGACCCC GTGGAAGAGA TGGCGAACCC 
GGTACTCCTG GGAACCCAGG ACCTGCTGGC CCGCCTGGAC CTCCTGGACC CCCTGGCCTT 
GGCGGAAACT TTGCTGCTCA AATGACAGGA GGATTTGATG AAAAAGCTGG CGGTGCACAA 
ATGGGAGTTA TGCAAGGACC AATGGGCCCT ATGGGACCAA GAGGACCTCC CGGCCCATCA 
GGAGCTCCTG GACCTCAAGG TTTCCAAGGG AATCCCGGTG AACCTGGTGA ATCTGGTGCT 
GGTGGTCCTA TGGGTCCTCG TGGTCCTCCT GGTCCTTCGG GAAAGCCTGG TGATGATGGT 
GAAGCTGGCA AACCTGGCAA ATCTGGTGAA CGTGGACCCC CAGGACCTCA GGGTGCTAGA 
GGTTTCCCAG GAACCCCAGG ACTTCCAGGA GTAAAAGGAC ACAGGGGTTA CCCAGGTCTT 
GATGGTGGAA AGGGTGAAGC TGGTGCTGCA GGTGCCAAGG GAGAAAGTGG AGCTTCTGGT 
GAAGCCGGAG CTCCTGGTCC AATGGGTCCC CGTGGTCTTC CAGGTGAGAG AGGACGTCCT 
GGATCTTCTG GTGCTGCTGG TGCTCGTGGT AATGATGGTC TCCCTGGTCC CGCTGGTCCA 
CCAGGACCTG TTGGCCCTGC TGGTGCTCCT GGTTTCCCTG GTGCCCCTGG CTCAAAGGGT 
GAAGCTGGCC CAACTGGAGC TCGTGGCCCT GAGGGTGCTC AAGGACCCAG AGGAGAATCT 
GGTACCCCTG GATCCCCTGG ACCTTCTGGA GCTTCTGGTA ACCCTGGTAC TGATGGTATT 
CCTGGCGCCA AAGGTTCATC TGGTGCTTCT GGTATTGCTG GTGCCCCTGG TTTCCCTGGA 
CCACGTGGTC CACCAGGACC TCAAGGAGCT ACTGGTCCTC TTGGTCCCAA AGGCGAGACT 
GGTGACCCTG GTATAGCAGG TTTCAAGGGT GAACATGGTC CCAAAGGTGA AATCGGGTCT 
GCAGGTCCTC AGGGTGCTCC TGGCCCAGCT GGAGAAGAAG GCAAAAGAGG AGCTCGTGGT 
GAACCTGGTG CTGCTGGGCC ACTTGGTCCT CCTGGTGAGA GAGGTGCTCC TGGTAATCGT 
GGTTTCCCTG GTCAAGATGG TCTTGCTGGT CCTAAGGGTG CTCCTGGTGA ACGTGGTGTT 
CCAGGTCTTG GTGGACCAAA GGGTGGTAAT GGTGATCCTG GCCGTCCCGG TGAACCCGGT 
CTCCCTGGTG CTAGAGGTCT TACTGGCCGT CCTGGTGATG CTGGTCCTCA GGGAAAAGTT 
GGGCCCTCTG GTGCTGCTGG TGAAGATGGT CGTCCAGGAC CTCCTGGGCC ACAAGGTGCT 
CGCGGTCAGC CTGGTGTTAT GGGATTCCCT GGACCTAAGG GTGCCAATGG TGAACCTGGC 
AAATCTGGTG AGAAAGGACT TGGTGGTGCT CCTGGTTTGA GGGGTTTGCC TGGAAAAGAT 
GGTGAAACTG GTGCTCAAGG TCCCAATGGT CCAGCTGGAC CTGCTGGTGA GAGAGGTGAA 
CAGGGACCTC CTGGTCCATC TGGCTTCCAG GGACTTCCTG GACCTCCCGG TTCTCCCGGA 
GAAGGTGGCA AACCAGGAGA TCAGGGTGTG CCCGGAGAAG CAGGTGCAGC AGGTCTTGTT 
GGACCAAGAG GTGAACGTGG TTTCCCAGGT GAGCGTGGGT CTTCCGGTCC TCAAGGTCTT 
CAGGGTTCTC GTGGTCTTCC TGGAACTCCT GGTACTGATG GTCCCAAGGG TGCAACTGGT 
CCATCTGGTC CCAATGGTGC CCAAGGTCCT CCTGGTCTTC AAGGTATGCC TGGTGAGAGA 
GGAGCTGCTG GTATTTCTGG ACCCAAAGGT GATAGAGGTG ATACTGGTGA GAAAGGCCCA 
GAGGGTGCTC CTGGCAAAGA TGGTTCAAGA GGTTTGACAG GTCCACTTGG TCCCCCTGGT 
CCAGCTGGTC CTAATGGTGA GAAGGGTGAA TCTGGTCCTT CTGGTCCACC TGGTATTGTT 
GGTGCCCGTG GTGCCCCTGG TGATCGTGGT GAGAATGGCC CTCCAGGTCC TGCTGGCTTT 
GCTGGTCCTC CAGGTTCTGA TGGTCAAGCT GGTCTTAAGG GTGATCAAGG TGAATCTGGC 
CAGAAGGGTG ATGCTGGTGC TCCTGGTCCT CAGGGTCCAT CTGGTGCTCC TGGCCCACAG 
GGCCCAACTG GTGTTAATGG TCCTAAAGGA GCTCGTGGTG CTCAAGGTCC TGCTGGTGCT 
ACTGGATTCC CTGGCGCTGC TGGAAGAGTT GGCACCCCAG GTCCCAATGG TAACCCTGGA 
CCTCCTGGTC CTCCCGGATC TGCTGGAAAA GAAGGACCAA AGGGTGTACG TGGTGATGCT 
GGTCCTACTG GTCGTGCTGG TGATCCAGGT CTTCAGGGTC CTGCTGGTGC TCCTGGAGAG 
AAGGGAGAGC CTGGTGAAGA TGGTCCTTCT GGTCCAGATG GTCCCAGTGG TCCTCAAGGT 
TTGTCTGGAA ATCGTGGTAT TGTTGGTCTA CCAGGTCAGC GTGGTGAAAG AGGATTCCCT 
GGACTTCCTG GCCCATCTGG TGAACCTGGC AAACAAGGAG GCCCTGGATC TTCAGGAGAC 
CGTGGACCCC CTGGCCCTGT TGGCCCACCT GGTTTGACTG GACCTGCTGG AGAGACTGGA 
CGAGAAGGTA ATCCCGGATC TGATGGCCCA CCTGGTCGAG ATGGTGCTAC TGGAATTAAG 
GGTGATCGTG GTGAAACTGG ACCAATTGGC GCTCCTGGTG CTCCAGGTTC CCCTGGTGCT 
CCTGGACCTG TTGGCCCAAC TGGAAAACAA GGAGACAGAG GAGAATCTGG ATCCCAAGGT 
CCACTTGGTC CTTCTGGTCC TGCTGGTGCT CGAGGTTTGA ATGGTCCTCA AGGTCCACGT 
GGTGATAAGG GTGAAGCTGG TGAGGCAGGA GAGAGAGGAC AGAAAGGTCA CAGAGGATTC 
ACTGGTCTTC AGGGTCTACC CGGTCCCCCT GGTACTGCTG GTGATCAAGG TGCTACTGGT 
TCTGCCGGTC CTTCTGGTCC TAGAGGTCCC CCTGGCCCTG TTGGTCCTTC TGGAAAAGAT 
GGCTCTAATG GTATCCCTGG TCCAATTGGT CCACCTGGTC CACGCGGTCG TAGTGGTGAA 
ACTGGCCCAG CTGGTCCACC TGGTCAATCT GGCCCACCTG GCCCCCCTGG TCCTCCTGGG 
CCTGGAATTG ACATGTCTGC TTTTGCTGGC CTATCTCAAC CTGAAAAAGG ACCTGACCCA 
ATGCGCTACA TGCGTGCTGA CCAAGCCTCT AGTTCTGTCC TTCAGACCGC TGATGTTGAA 
GCCAATTTGA AAACCCTGAA TAACCAGATC GAAAGCATTC GCAGCCCAGA TGGTACCAAA 
AAGAACCCAG CCCGCACATG CCGTGACCTG AAACTCTGCC ATCCTGAATG GAAGAGTGGT 
GAATATTGGA TTGATCCCAA CGAGGGCTGC ACTGTAGATG CTATCAAAGT CTTCTGCAAC 
ATGGAGACTG GAGAGTCCTG TGTATACCCC AGCCCATCAA AGATCCCCAA GAAGAACTGG 
TGGAGCGCCA AGGGCAAGGA AAAGAAGCAC ATTTGGTTTG GAGAGACAAT CAATGGTGGT 
TTCCAATTCA GCTATGGAGA TGACAGCTCA GCACCCAACA CTGCTAACAT TCAGTTGACC 
TTCCTGCGTC TGCTGTCCAC TGATGCATCC CAGAACATCA CCTATCACTG CAAGAATAGC 
ATTGCATTTA TGGATGAAGC TTCAGGCAAC CTTAAGAAGG CTGTTCTACT CCAAGGATCC 
AATGATGTAG AAATCAGAGC TGAAGGCAAC AGCAGATTCA CTTACAATGC CTTGGAAGAT 
GGCTGCAAGA AACACACTGG CAAATGGAGC AAGACAGTTA TTGAATATAG GACACAGAAA 
ACATCTCGCC TGCCCATCGT AGACATTGCA CCTATGGATA TTGGTGGCGC TGATCAGGAA 
TTTGGTGTTG ACATTGGCCC AGTCTGCTTC TTGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001081260.1
CDS	147..4622
Translation	MFSFVDSRTLVLFAATQVILLAVVRCQDEEDVLDTGSCVQDGQRYSDKDVWKPEPCQICVCDTGTVLCDEIICEESKDCPNAEIPFGECCPICPTEQSSTSSGQGVAKGQKGEPGDIKDVVGPRGPPGPQGPSGEQGPRGERGDKGETGAPGPRGRDGEPGTPGNPGPAGPPGPPGPPGLGGNFAAQMTGGFDEKAGGAQMGVMQGPMGPMGPRGPPGPSGAPGPQGFQGNPGEPGESGAGGPMGPRGPPGPSGKPGDDGEAGKPGKSGERGPPGPQGARGFPGTPGLPGVKGHRGYPGLDGGKGEAGAAGAKGESGASGEAGAPGPMGPRGLPGERGRPGSSGAAGARGNDGLPGPAGPPGPVGPAGAPGFPGAPGSKGEAGPTGARGPEGAQGPRGESGTPGSPGPSGASGNPGTDGIPGAKGSSGASGIAGAPGFPGPRGPPGPQGATGPLGPKGETGDPGIAGFKGEHGPKGEIGSAGPQGAPGPAGEEGKRGARGEPGAAGPLGPPGERGAPGNRGFPGQDGLAGPKGAPGERGVPGLGGPKGGNGDPGRPGEPGLPGARGLTGRPGDAGPQGKVGPSGAAGEDGRPGPPGPQGARGQPGVMGFPGPKGANGEPGKSGEKGLGGAPGLRGLPGKDGETGAQGPNGPAGPAGERGEQGPPGPSGFQGLPGPPGSPGEGGKPGDQGVPGEAGAAGLVGPRGERGFPGERGSSGPQGLQGSRGLPGTPGTDGPKGATGPSGPNGAQGPPGLQGMPGERGAAGISGPKGDRGDTGEKGPEGAPGKDGSRGLTGPLGPPGPAGPNGEKGESGPSGPPGIVGARGAPGDRGENGPPGPAGFAGPPGSDGQAGLKGDQGESGQKGDAGAPGPQGPSGAPGPQGPTGVNGPKGARGAQGPAGATGFPGAAGRVGTPGPNGNPGPPGPPGSAGKEGPKGVRGDAGPTGRAGDPGLQGPAGAPGEKGEPGEDGPSGPDGPSGPQGLSGNRGIVGLPGQRGERGFPGLPGPSGEPGKQGGPGSSGDRGPPGPVGPPGLTGPAGETGREGNPGSDGPPGRDGATGIKGDRGETGPIGAPGAPGSPGAPGPVGPTGKQGDRGESGSQGPLGPSGPAGARGLNGPQGPRGDKGEAGEAGERGQKGHRGFTGLQGLPGPPGTAGDQGATGSAGPSGPRGPPGPVGPSGKDGSNGIPGPIGPPGPRGRSGETGPAGPPGQSGPPGPPGPPGPGIDMSAFAGLSQPEKGPDPMRYMRADQASSSVLQTADVEANLKTLNNQIESIRSPDGTKKNPARTCRDLKLCHPEWKSGEYWIDPNEGCTVDAIKVFCNMETGESCVYPSPSKIPKKNWWSAKGKEKKHIWFGETINGGFQFSYGDDSSAPNTANIQLTFLRLLSTDASQNITYHCKNSIAFMDEASGNLKKAVLLQGSNDVEIRAEGNSRFTYNALEDGCKKHTGKWSKTVIEYRTQKTSRLPIVDIAPMDIGGADQEFGVDIGPVCFL

Target ORF information:

RefSeq Version	NM_001087791.1
Organism	Xenopus laevis(African clawed frog)
Definition	Xenopus laevis collagen, type II, alpha 1 S homeolog (col2a1.S), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001087791.1

ORF Insert Sequence:

ATGTTTAGCT TTGTGGATTC AAGGACTCTA GTGCTGTTCG CAGCCACACA AGTCATTTTA 
TTAGCGGTTG TACGATGCCA AGATGAAGAA GATGTCCTGG ATACAGGCAG CTGCGTGCAG 
GATGGGCAGA GGTATAGTGA TAAAGATGTG TGGAAACCCG AGCCCTGCCA AATCTGTGTC 
TGTGACACTG GGACTGTTCT CTGCGATGAA ATAATCTGCG AAGAGTCCAA AGATTGCCCC 
AATGCTGAGA TCCCCTTCGG AGAGTGCTGT CCCATCTGCC CAACTGAGCA ATCTTCTACC 
TCCAGTGGGC AAGGAGTGGC CAAGGGACAG AAAGGTGAAC CTGGTGATAT TAAAGATGTT 
GTAGGACCAA GAGGACCACC TGGACCACAG GGACCTTCTG GTGAACAAGG ACCTAGAGGA 
GAACGTGGAG ATAAGGGCGA GACTGGTGCT CCCGGACCCC GTGGAAGAGA TGGCGAACCC 
GGTACTCCTG GGAACCCAGG ACCTGCTGGC CCGCCTGGAC CTCCTGGACC CCCTGGCCTT 
GGCGGAAACT TTGCTGCTCA AATGACAGGA GGATTTGATG AAAAAGCTGG CGGTGCACAA 
ATGGGAGTTA TGCAAGGACC AATGGGCCCT ATGGGACCAA GAGGACCTCC CGGCCCATCA 
GGAGCTCCTG GACCTCAAGG TTTCCAAGGG AATCCCGGTG AACCTGGTGA ATCTGGTGCT 
GGTGGTCCTA TGGGTCCTCG TGGTCCTCCT GGTCCTTCGG GAAAGCCTGG TGATGATGGT 
GAAGCTGGCA AACCTGGCAA ATCTGGTGAA CGTGGACCCC CAGGACCTCA GGGTGCTAGA 
GGTTTCCCAG GAACCCCAGG ACTTCCAGGA GTAAAAGGAC ACAGGGGTTA CCCAGGTCTT 
GATGGTGGAA AGGGTGAAGC TGGTGCTGCA GGTGCCAAGG GAGAAAGTGG AGCTTCTGGT 
GAAGCCGGAG CTCCTGGTCC AATGGGTCCC CGTGGTCTTC CAGGTGAGAG AGGACGTCCT 
GGATCTTCTG GTGCTGCTGG TGCTCGTGGT AATGATGGTC TCCCTGGTCC CGCTGGTCCA 
CCAGGACCTG TTGGCCCTGC TGGTGCTCCT GGTTTCCCTG GTGCCCCTGG CTCAAAGGGT 
GAAGCTGGCC CAACTGGAGC TCGTGGCCCT GAGGGTGCTC AAGGACCCAG AGGAGAATCT 
GGTACCCCTG GATCCCCTGG ACCTTCTGGA GCTTCTGGTA ACCCTGGTAC TGATGGTATT 
CCTGGCGCCA AAGGTTCATC TGGTGCTTCT GGTATTGCTG GTGCCCCTGG TTTCCCTGGA 
CCACGTGGTC CACCAGGACC TCAAGGAGCT ACTGGTCCTC TTGGTCCCAA AGGCGAGACT 
GGTGACCCTG GTATAGCAGG TTTCAAGGGT GAACATGGTC CCAAAGGTGA AATCGGGTCT 
GCAGGTCCTC AGGGTGCTCC TGGCCCAGCT GGAGAAGAAG GCAAAAGAGG AGCTCGTGGT 
GAACCTGGTG CTGCTGGGCC ACTTGGTCCT CCTGGTGAGA GAGGTGCTCC TGGTAATCGT 
GGTTTCCCTG GTCAAGATGG TCTTGCTGGT CCTAAGGGTG CTCCTGGTGA ACGTGGTGTT 
CCAGGTCTTG GTGGACCAAA GGGTGGTAAT GGTGATCCTG GCCGTCCCGG TGAACCCGGT 
CTCCCTGGTG CTAGAGGTCT TACTGGCCGT CCTGGTGATG CTGGTCCTCA GGGAAAAGTT 
GGGCCCTCTG GTGCTGCTGG TGAAGATGGT CGTCCAGGAC CTCCTGGGCC ACAAGGTGCT 
CGCGGTCAGC CTGGTGTTAT GGGATTCCCT GGACCTAAGG GTGCCAATGG TGAACCTGGC 
AAATCTGGTG AGAAAGGACT TGGTGGTGCT CCTGGTTTGA GGGGTTTGCC TGGAAAAGAT 
GGTGAAACTG GTGCTCAAGG TCCCAATGGT CCAGCTGGAC CTGCTGGTGA GAGAGGTGAA 
CAGGGACCTC CTGGTCCATC TGGCTTCCAG GGACTTCCTG GACCTCCCGG TTCTCCCGGA 
GAAGGTGGCA AACCAGGAGA TCAGGGTGTG CCCGGAGAAG CAGGTGCAGC AGGTCTTGTT 
GGACCAAGAG GTGAACGTGG TTTCCCAGGT GAGCGTGGGT CTTCCGGTCC TCAAGGTCTT 
CAGGGTTCTC GTGGTCTTCC TGGAACTCCT GGTACTGATG GTCCCAAGGG TGCAACTGGT 
CCATCTGGTC CCAATGGTGC CCAAGGTCCT CCTGGTCTTC AAGGTATGCC TGGTGAGAGA 
GGAGCTGCTG GTATTTCTGG ACCCAAAGGT GATAGAGGTG ATACTGGTGA GAAAGGCCCA 
GAGGGTGCTC CTGGCAAAGA TGGTTCAAGA GGTTTGACAG GTCCACTTGG TCCCCCTGGT 
CCAGCTGGTC CTAATGGTGA GAAGGGTGAA TCTGGTCCTT CTGGTCCACC TGGTATTGTT 
GGTGCCCGTG GTGCCCCTGG TGATCGTGGT GAGAATGGCC CTCCAGGTCC TGCTGGCTTT 
GCTGGTCCTC CAGGTTCTGA TGGTCAAGCT GGTCTTAAGG GTGATCAAGG TGAATCTGGC 
CAGAAGGGTG ATGCTGGTGC TCCTGGTCCT CAGGGTCCAT CTGGTGCTCC TGGCCCACAG 
GGCCCAACTG GTGTTAATGG TCCTAAAGGA GCTCGTGGTG CTCAAGGTCC TGCTGGTGCT 
ACTGGATTCC CTGGCGCTGC TGGAAGAGTT GGCACCCCAG GTCCCAATGG TAACCCTGGA 
CCTCCTGGTC CTCCCGGATC TGCTGGAAAA GAAGGACCAA AGGGTGTACG TGGTGATGCT 
GGTCCTACTG GTCGTGCTGG TGATCCAGGT CTTCAGGGTC CTGCTGGTGC TCCTGGAGAG 
AAGGGAGAGC CTGGTGAAGA TGGTCCTTCT GGTCCAGATG GTCCCAGTGG TCCTCAAGGT 
TTGTCTGGAA ATCGTGGTAT TGTTGGTCTA CCAGGTCAGC GTGGTGAAAG AGGATTCCCT 
GGACTTCCTG GCCCATCTGG TGAACCTGGC AAACAAGGAG GCCCTGGATC TTCAGGAGAC 
CGTGGACCCC CTGGCCCTGT TGGCCCACCT GGTTTGACTG GACCTGCTGG AGAGACTGGA 
CGAGAAGGTA ATCCCGGATC TGATGGCCCA CCTGGTCGAG ATGGTGCTAC TGGAATTAAG 
GGTGATCGTG GTGAAACTGG ACCAATTGGC GCTCCTGGTG CTCCAGGTTC CCCTGGTGCT 
CCTGGACCTG TTGGCCCAAC TGGAAAACAA GGAGACAGAG GAGAATCTGG ATCCCAAGGT 
CCACTTGGTC CTTCTGGTCC TGCTGGTGCT CGAGGTTTGA ATGGTCCTCA AGGTCCACGT 
GGTGATAAGG GTGAAGCTGG TGAGGCAGGA GAGAGAGGAC AGAAAGGTCA CAGAGGATTC 
ACTGGTCTTC AGGGTCTACC CGGTCCCCCT GGTACTGCTG GTGATCAAGG TGCTACTGGT 
TCTGCCGGTC CTTCTGGTCC TAGAGGTCCC CCTGGCCCTG TTGGTCCTTC TGGAAAAGAT 
GGCTCTAATG GTATCCCTGG TCCAATTGGT CCACCTGGTC CACGCGGTCG TAGTGGTGAA 
ACTGGCCCAG CTGGTCCACC TGGTCAATCT GGCCCACCTG GCCCCCCTGG TCCTCCTGGG 
CCTGGAATTG ACATGTCTGC TTTTGCTGGC CTATCTCAAC CTGAAAAAGG ACCTGACCCA 
ATGCGCTACA TGCGTGCTGA CCAAGCCTCT AGTTCTGTCC TTCAGACCGC TGATGTTGAA 
GCCAATTTGA AAACCCTGAA TAACCAGATC GAAAGCATTC GCAGCCCAGA TGGTACCAAA 
AAGAACCCAG CCCGCACATG CCGTGACCTG AAACTCTGCC ATCCTGAATG GAAGAGTGGT 
GAATATTGGA TTGATCCCAA CGAGGGCTGC ACTGTAGATG CTATCAAAGT CTTCTGCAAC 
ATGGAGACTG GAGAGTCCTG TGTATACCCC AGCCCATCAA AGATCCCCAA GAAGAACTGG 
TGGAGCGCCA AGGGCAAGGA AAAGAAGCAC ATTTGGTTTG GAGAGACAAT CAATGGTGGT 
TTCCAATTCA GCTATGGAGA TGACAGCTCA GCACCCAACA CTGCTAACAT TCAGTTGACC 
TTCCTGCGTC TGCTGTCCAC TGATGCATCC CAGAACATCA CCTATCACTG CAAGAATAGC 
ATTGCATTTA TGGATGAAGC TTCAGGCAAC CTTAAGAAGG CTGTTCTACT CCAAGGATCC 
AATGATGTAG AAATCAGAGC TGAAGGCAAC AGCAGATTCA CTTACAATGC CTTGGAAGAT 
GGCTGCAAGA AACACACTGG CAAATGGAGC AAGACAGTTA TTGAATATAG GACACAGAAA 
ACATCTCGCC TGCCCATCGT AGACATTGCA CCTATGGATA TTGGTGGCGC TGATCAGGAA 
TTTGGTGTTG ACATTGGCCC AGTCTGCTTC TTGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Genetic and genomic tools for Xenopus research: The NIH Xenopus initiative.
Developmental dynamics : an official publication of the American Association of Anatomists225(4)384-91(2002 Dec)
Klein SL,Strausberg RL,Wagner L,Pontius J,Clifton SW,Richardson P

Expression of two nonallelic type II procollagen genes during Xenopus laevis embryogenesis is characterized by stage-specific production of alternatively spliced transcripts.
The Journal of cell biology115(2)565-75(1991 Oct)
Su MW,Suzuki HR,Bieker JJ,Solursh M,Ramirez F

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col2a1.S cDNA ORF clone, Xenopus laevis(African clawed frog)

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gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

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