map2k1.L cDNA ORF clone, Xenopus laevis(African clawed frog)

Gene
Clones
gRNAs

Summary

Gene Symbol	map2k1.L
Entrez Gene ID	379991
Full Name	mitogen-activated protein kinase kinase 1 L homeolog
Synonyms	map2k1,mek-2
General protein information	Preferred Names mitogen-activated protein kinase kinase 1 L homeolog Names dual specificity mitogen-activated protein kinase kinase 1 ERK activator kinase 1 MAP kinase kinase 1 MAP kinase kinase or Erk Kinase, Dual specificity mitogen-activated protein kinase kinase, involved in ras mediated vulval induction, LEThal LET-537 (42.8 kD) (mek-2) MAPK/ERK kinase 1 MAPKK 1 MEK1
Gene Type	protein-coding
Organism	Xenopus laevis(African clawed frog)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001086830.1	NP_001080299.1	dual specificity mitogen-activated protein kinase kinase 1

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Vg1RBP phosphorylation by Erk2 MAP kinase correlates with the cortical release of Vg1 mRNA during meiotic maturation of Xenopus oocytes.
Git A, Allison R, Perdiguero E, Nebreda AR, Houliston E, Standart N
RNA (New York, N.Y.)15(6)1121-33(2009 Jun)

ERK modulates DNA bending and enhancesome structure by phosphorylating HMG1-boxes 1 and 2 of the RNA polymerase I transcription factor UBF.
Stefanovsky VY, Langlois F, Bazett-Jones D, Pelletier G, Moss T
Biochemistry45(11)3626-34(2006 Mar)

ERK2 is required for FGF1-induced JNK1 phosphorylation in Xenopus oocyte expressing FGF receptor 1.
Browaeys-Poly E, Fafeur V, Vilain JP, Cailliau K
Biochimica et biophysica acta1743(1-2)1-4(2005 Mar)

Genetic and genomic tools for Xenopus research: The NIH Xenopus initiative.
Klein SL, Strausberg RL, Wagner L, Pontius J, Clifton SW, Richardson P
Developmental dynamics : an official publication of the American Association of Anatomists225(4)384-91(2002 Dec)

cDNA cloning of MAP kinase kinase reveals kinase cascade pathways in yeasts to vertebrates.
Kosako H, Nishida E, Gotoh Y
The EMBO journal12(2)787-94(1993 Feb)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following map2k1.L gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the map2k1.L cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OAf00483	NM_001086830.1 Latest version!	Xenopus laevis mitogen-activated protein kinase kinase 1 L homeolog (map2k1.L), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

map2k1.L ( NM_001086830.1 ) cDNA ORF clone, Xenopus laevis(African clawed frog) -> NP_001080299.1 Xenopus laevis mitogen-activated protein kinase kinase 1 L homeolog (map2k1.L), mRNA.

Price & Availability↑

CloneID	OAf00483
Clone ID Related Accession (Same CDS sequence)	NM_001086830.1
Accession Version	NM_001086830.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1188bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1550246400000
Organism	Xenopus laevis(African clawed frog)
Product	dual specificity mitogen-activated protein kinase kinase 1
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC043913.1. ##Evidence-Data-START## Transcript exon combination :: BC043913.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMD00012418, SAMD00012419 [ECO:0000348] ##Evidence-Data-END##

ATGCCTAAAA AGAAGCCTAC GCCCATACAG CTGAATCCCA ACCCCGAAGG GACTGCTGTG 
AACGGGACCC CTACAGCCGA GACAAACCTT GAAGCTCTGC AGAAAAAGTT GGAAGAGCTT 
GAGCTGGATG AGCAGCAGAG GAAGCGTCTG GAGGCTTTTC TCACCCAGAA GCAGAAAGTT 
GGGGAACTGA AGGATGACGA CTTTGAAAAA GTTTCAGAGC TTGGAGCAGG CAACGGAGGA 
GTGGTGTTTA AGGTGTCCCA CAAGCCAACC AGCTTGATTA TGGCCAGGAA GTTGATTCAT 
CTGGAGATTA AGCCTGCAAT CCGAAACCAG ATTATCCGAG AGTTGCAGGT TCTGCATGAA 
TGTAACTCCC CATACATTGT GGGGTTCTAT GGGGCCTTCT ACAGTGATGG AGAGATCAGC 
ATTTGCATGG AACACATGGA TGGAGGCTCC CTTGATCAGG TTCTGAAGAA AGCTGGCAAA 
ATCCCAGAAA AGATTTTGGG AAAAGTCAGC ATTGCAGTGA TAAAAGGTCT AACCTACCTG 
AGAGAAAAGC ATAAGATAAT GCACAGAGAT GTGAAACCTT CTAACATCCT GGTCAACTCT 
AGAGGAGAGA TAAAACTCTG CGACTTTGGG GTCAGCGGGC AACTCATAGA CTCCATGGCA 
AATTCCTTTG TTGGGACAAG ATCCTATATG TCACCGGAGC GACTACAGGG CACTCATTAT 
TCTGTGCAAT CAGACATCTG GAGCATGGGG CTGTCGCTGG TGGAAATGGC CATTGGAAGG 
TATCCCATTC CACCCCCTGA TGCCAAAGAG CTGGAACTTA TCTTTGGGTG TTCTGTAGAA 
AGGGATCCAG CATCTTCTGA ACTGGCACCT CGCCCCCGGC CACCCGGACG TCCAATAAGC 
TCATACGGTC CTGATAGTCG ACCACCCATG GCTATTTTTG AACTTCTGGA TTATATCGTG 
AACGAGCCGC CTCCAAAATT GCCCAGTGGA GTATTTGGAG CTGAGTTCCA GGACTTTGTG 
AATAAATGTC TTGTGAAGAA TCCGGCAGAG AGAGCAGACC TTAAACAGCT AATGGTTCAC 
AGCTTCATTA AGCAGTCAGA GTTGGAGGAA GTGGATTTTG CTGGATGGCT CTGTTCCACT 
ATGGGCCTTA AGCAGCCCAG TACCCCAACC CATGCCGCCG GAGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001080299.1
CDS	100..1287
Translation	MPKKKPTPIQLNPNPEGTAVNGTPTAETNLEALQKKLEELELDEQQRKRLEAFLTQKQKVGELKDDDFEKVSELGAGNGGVVFKVSHKPTSLIMARKLIHLEIKPAIRNQIIRELQVLHECNSPYIVGFYGAFYSDGEISICMEHMDGGSLDQVLKKAGKIPEKILGKVSIAVIKGLTYLREKHKIMHRDVKPSNILVNSRGEIKLCDFGVSGQLIDSMANSFVGTRSYMSPERLQGTHYSVQSDIWSMGLSLVEMAIGRYPIPPPDAKELELIFGCSVERDPASSELAPRPRPPGRPISSYGPDSRPPMAIFELLDYIVNEPPPKLPSGVFGAEFQDFVNKCLVKNPAERADLKQLMVHSFIKQSELEEVDFAGWLCSTMGLKQPSTPTHAAGV

Target ORF information:

RefSeq Version	NM_001086830.1
Organism	Xenopus laevis(African clawed frog)
Definition	Xenopus laevis mitogen-activated protein kinase kinase 1 L homeolog (map2k1.L), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001086830.1

ORF Insert Sequence:

ATGCCTAAAA AGAAGCCTAC GCCCATACAG CTGAATCCCA ACCCCGAAGG GACTGCTGTG 
AACGGGACCC CTACAGCCGA GACAAACCTT GAAGCTCTGC AGAAAAAGTT GGAAGAGCTT 
GAGCTGGATG AGCAGCAGAG GAAGCGTCTG GAGGCTTTTC TCACCCAGAA GCAGAAAGTT 
GGGGAACTGA AGGATGACGA CTTTGAAAAA GTTTCAGAGC TTGGAGCAGG CAACGGAGGA 
GTGGTGTTTA AGGTGTCCCA CAAGCCAACC AGCTTGATTA TGGCCAGGAA GTTGATTCAT 
CTGGAGATTA AGCCTGCAAT CCGAAACCAG ATTATCCGAG AGTTGCAGGT TCTGCATGAA 
TGTAACTCCC CATACATTGT GGGGTTCTAT GGGGCCTTCT ACAGTGATGG AGAGATCAGC 
ATTTGCATGG AACACATGGA TGGAGGCTCC CTTGATCAGG TTCTGAAGAA AGCTGGCAAA 
ATCCCAGAAA AGATTTTGGG AAAAGTCAGC ATTGCAGTGA TAAAAGGTCT AACCTACCTG 
AGAGAAAAGC ATAAGATAAT GCACAGAGAT GTGAAACCTT CTAACATCCT GGTCAACTCT 
AGAGGAGAGA TAAAACTCTG CGACTTTGGG GTCAGCGGGC AACTCATAGA CTCCATGGCA 
AATTCCTTTG TTGGGACAAG ATCCTATATG TCACCGGAGC GACTACAGGG CACTCATTAT 
TCTGTGCAAT CAGACATCTG GAGCATGGGG CTGTCGCTGG TGGAAATGGC CATTGGAAGG 
TATCCCATTC CACCCCCTGA TGCCAAAGAG CTGGAACTTA TCTTTGGGTG TTCTGTAGAA 
AGGGATCCAG CATCTTCTGA ACTGGCACCT CGCCCCCGGC CACCCGGACG TCCAATAAGC 
TCATACGGTC CTGATAGTCG ACCACCCATG GCTATTTTTG AACTTCTGGA TTATATCGTG 
AACGAGCCGC CTCCAAAATT GCCCAGTGGA GTATTTGGAG CTGAGTTCCA GGACTTTGTG 
AATAAATGTC TTGTGAAGAA TCCGGCAGAG AGAGCAGACC TTAAACAGCT AATGGTTCAC 
AGCTTCATTA AGCAGTCAGA GTTGGAGGAA GTGGATTTTG CTGGATGGCT CTGTTCCACT 
ATGGGCCTTA AGCAGCCCAG TACCCCAACC CATGCCGCCG GAGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Vg1RBP phosphorylation by Erk2 MAP kinase correlates with the cortical release of Vg1 mRNA during meiotic maturation of Xenopus oocytes.
RNA (New York, N.Y.)15(6)1121-33(2009 Jun)
Git A,Allison R,Perdiguero E,Nebreda AR,Houliston E,Standart N

ERK modulates DNA bending and enhancesome structure by phosphorylating HMG1-boxes 1 and 2 of the RNA polymerase I transcription factor UBF.
Biochemistry45(11)3626-34(2006 Mar)
Stefanovsky VY,Langlois F,Bazett-Jones D,Pelletier G,Moss T

ERK2 is required for FGF1-induced JNK1 phosphorylation in Xenopus oocyte expressing FGF receptor 1.
Biochimica et biophysica acta1743(1-2)1-4(2005 Mar)
Browaeys-Poly E,Fafeur V,Vilain JP,Cailliau K

Genetic and genomic tools for Xenopus research: The NIH Xenopus initiative.
Developmental dynamics : an official publication of the American Association of Anatomists225(4)384-91(2002 Dec)
Klein SL,Strausberg RL,Wagner L,Pontius J,Clifton SW,Richardson P

cDNA cloning of MAP kinase kinase reveals kinase cascade pathways in yeasts to vertebrates.
The EMBO journal12(2)787-94(1993 Feb)
Kosako H,Nishida E,Gotoh Y

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map2k1.L cDNA ORF clone, Xenopus laevis(African clawed frog)

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Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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