col4a2.L cDNA ORF clone, Xenopus laevis(African clawed frog)

Gene
Clones
gRNAs

Summary

Gene Symbol	col4a2.L
Entrez Gene ID	373763
Full Name	collagen, type IV, alpha 2 L homeolog
Synonyms	col4a2,col4a2-a
General protein information	Preferred Names collagen, type IV, alpha 2 L homeolog Names collagen alpha-2(IV) chain collagen alpha2(IV)
Gene Type	protein-coding
Organism	Xenopus laevis(African clawed frog)

mRNA and Protein(s)

mRNA	Protein	Name
XM_018247204.1	XP_018102693.1	collagen alpha-2(IV) chain

Pathways from BioSystems
Gene Ontology

The following col4a2.L gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the col4a2.L cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OAf20554	XM_018247204.1 Latest version!	Xenopus laevis collagen, type IV, alpha 2 L homeolog (col4a2.L), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00

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Next-day Shipping ORF Clones ( in default vector with tag)
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2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

col4a2.L ( XM_018247204.1 ) cDNA ORF clone, Xenopus laevis(African clawed frog) -> XP_018102693.1 Xenopus laevis collagen, type IV, alpha 2 L homeolog (col4a2.L), mRNA.

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CloneID	OAf20554
Clone ID Related Accession (Same CDS sequence)	XM_018247204.1
Accession Version	XM_018247204.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5172bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1474214400000
Organism	Xenopus laevis(African clawed frog)
Product	collagen alpha-2(IV) chain
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030726.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus laevis Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACAAGAA CTCAAAGCAC CAAAAGATGT GCCACAAGCA GCAAAGAGCA TAACCATTTT 
CTACTCAAAC ATCATTCAGT CACTTGGTAC GTGATGTTCG TTGTACTGGT GACACTTTTT 
GCTGGAAATG CCCATGCTGG TGACAAGTCA TATACAGGGC CCTGTGGAGG AAGAGACTGC 
AGTCAGGGGT GCCAGTGTCT GCCGGAGAAA GGAAGTCGCG GACAACCAGG ACCTCTTGGC 
CCGCAAGGTA CCCCTGGCCC ACCAGGACTC ATAGGAATAG CAGGTCTCCA GGGACGTAAG 
GGAGACAAAG GAGAGCGAGG ATTTCCTGGA GTGACTGGAC CCTTTGGAGA CAAGGGCCAA 
AGAGGTGTTA CAGGTTTTTC CGGAGCTGAT GGCATACCTG GACATACAGG TCAGGGTGGC 
TCAAGAGGTA AACCAGGTCA TGATGGTTGT AATGGAACAA AAGGTGATGC TGGTGATGGG 
GGCTCACATG GTCCAAGAGG CCCTCCTGGA ATTCTTGGTG GATTTGGTGT CAAAGGCCAA 
AAAGGAGAAC CATTTCATGT ATCCATTGAG GTGAAAAACA GACTTAGAGG AGATCCTGGT 
GAACCAGGCT TTAATGGCAT TCAGGGTCCA AGGGGTTCTC CTGGAATAGC AGGATTCATT 
GGGCCACAGG GTTATCGTGG CCCTCGTGGG CCACCTGGAC CTCCTGGGAC ACAAGGACTA 
CAAGGCAACA GAGGACTTGG CTATTACGGA GAAAAAGGAG ACACTGGTGA TCCAGGTCCA 
CCTGGTGAAC AAATTCCTCC AGGAGAGAGA GAACAACAGC ACAAAACTGA AATTCTCTTG 
CTAAAATATA AGGGGGCCAA GGGGGACCAA GGAGAAAGGG GAATTCGAGG TGATAAAGGT 
CAACAAGCTG TCACAGGAGA AAAAGGAGAA GAAGGAATCA CAGGATTTCC TGGACAAAGG 
GGATTGCCTG GTAATGATGG TGGCCGTGGA AATGCAGGAG AAATAGGGTT TCCTGGGTCA 
AATGGAGCAC CTGGATTACC TGGTTATAAG GGAATAAAGG GAGAACATGG GAACCGAGGA 
CCTGCAGGAC CTGTTACCTT TCTCAAAGGT CAAAAACCAA AAAAAGGTTA TGAAGGTGAT 
ATGGGATTTC CAGGACTTAA TGGGCTTTAT GGAGATGACG GAGACTTGGG AGATCCTGGT 
TTTCCAGGTC AGAATGGATT TCCAAGTACA TTTCCAGGAC GCACAGGTGA TCCAGGATTG 
AATGGTCAAA GGGGCCCCAA AGGTTACCAA GGTGATCCAG GCTTACCAGC ATTTCAGCCA 
GGACCCCCGG GTAAAGATGG AGAACCTGGC AATGAAGGAC CACCTGGCAA ACCTGGATAC 
CCAGGTGGTC GCATTGGGAT GAAAGGATTA CCTGGGCCTC CAGGAAGGGA TGGACATAGA 
GGATTTCCTG GTTCTCGGGG TTTCAAAGGA ATTAAAGGTG AACAAGGGGA ATGCAAGTGT 
TTTGCAGGAG ATGAAGCTGG TAGAGGCCCT GCTGGAGAGT CTGGTCCAGT GGGTTTCCCA 
GGTTTTCAGG GTCTGTCAGG CAGAAAAGGA GAAGCGGGAG ATAGAGGAGT ACAAGGGCTA 
CCAGGAACAC CAGGCACAGT GGGTCTGACT GGTCCTGCTG GGTTTCCAGG TCCTGCTGGA 
GAGAAGGGTG ATAAAGTAGT TGCTACAGAA AAAGGTTCCA AGGGAGTGCA AGGCAACTCT 
GGTTTTCCTG GTGCCCCTGG TCGTGATGGA TACCCGGGAA GAAATGGTAG AGATGGTATT 
CCTGGCTTTC AAGGCCCTCC TGGAGATGGT GTAAAAGGAC TCCCGGGTTA TGCTGGGTTT 
CCTGGACAAC CTGGTACTCC TGGTGTTCTA GGTGAGACAG GCTTTCCTGG ACCTGGATTT 
CCAGGTCCAA AAGGTGTACG TGGAGAACCA GGAAACAGAG GCATTAGTGG ATTCCCAGGA 
ATTCCTGGAC TTTCAGGTCC CCAAGGTGAC TGCTACCAAT CAGCAGATGA AGAAAATGAA 
ATAGGGAAAG AAGGAACTGG CTGCCATCTG ATTGCACCAC CTGCTCCAGA GGGCGCCCGA 
GGGCCACCAG GATTGCCAGG ATATAACGGT TTAAGAGGCC AGAAAGGTTT CCCTGGACCA 
CAAGGTTTTA ATGGAGTGTT TGGATTAAAA GGAGTCAAAG GAGAACCTGG GAATGATAGT 
TTGCCCGGTC CACCAGGTTT TCCTGGGCAA AGGGGAGATA GAGGTAATGC AGGAATTCCT 
GGTCTACCAG GGGCACCGGG TCTATCAGGA AAACCTGGCT TTAAAGGGGT TCAAGGAGAA 
AAGGGAGGTG CAGGTGATCG CTTAGGAGCT CCACCAGGGG AGCAAGGAAA TACTGGACTG 
CCTGGGTTGC AAGGACTGAA AGGATTACAA GGGGATCAAG GCATACCTGG TCTGCAAGGT 
AAAGTTGGAA TACCAGGAAC ACCTGGAAAC AAGGGTAGTA AAGGCAGTGC TGGGGCCCCA 
GGAGGACCTG GAATACCAGG ACCTGCAGCA CAATTTGGAT TTCCAGGAAT GCCAGGAAAC 
CCAGGCACTC CAGGACCCCA CGGTTCAATG GGACCTCCAG GTTCTCCAGG ACCGAGAGGA 
GATGAAGGCG ATAAAGGGCC ACCAGGTCCG GTTGGCATGA AAGGGTTACC TGGAGAACTA 
GGCAATGAAG GATTTGTTGG AATTAGGGGG AAACAAGGGG CTCCAGGTAT CCCTGGCTTG 
GTTGGAATGC ATGGACTTCC TGGTGTGAAA GGTGTCAAAG GTTCCCCTGG CATGGAAGGA 
TTTCATGGAA TGAAAGGAGC AAAGGGGAGA CCTGCATGGA GAGGACCCAA GGGACAAAGT 
GGCAATATAG GATTTCCAGG AGTAAAAGGG AGAGACGGAG CACTGGGTTA CAAAGGTGAC 
CGTGGGGAAA GGGGACTACC AGGAGAGCCC CCCGAAATGC TGCCAGAAGT TATGCATTTG 
GCAAAAGGTG AAAAGGGAGA TCAAGGTGTT CCTGGATTCA AGGGCACTAC TGGACCTACA 
GGCAGTAAAG GTATGCCAGG AGATCCTGGA CAGCCAGGGT TATCTGGTTT GCCTGGCATA 
CCAAGCTTTG AATTTGGTGA TAAAGGAGAT ACTGGCTTGC CTGGGCGTGG TGGTCATCCA 
GGAATTCCAG GAGAAGTTGG TCCACGAGGA ATTATTGGCT TCCCAGGATT TACTGGTCCT 
AGGGGAACTC CTGGTTTCTC TGGCCTTCCT GGTGTTCCTG GTGAAAGAGG CCATTATGGA 
GATACAGGCC AGAGAGGTGA TACTATTGAT TTACCAGGAG AAAGAGGAGT TAAGGGTCAG 
ACTGGAAGCC CTGGGCTGTC AGGACAACAT GGAATACATG GAGAAAAAGG AATAAAAGGG 
GAAGCCGGTT CCTATGGTAT TGAGGGAATG AGAGGTGAAC CTGGACAACC AGGAGAAAAG 
GGATTCCCAG GTCAGCAAGG CCTGTTGGGC TTACAAGGCT CACAGGGTTC ACCGGGTCAA 
CCGGGTGTAC ATGGCGAGAA AGGAAATACT GGGTTTCCTG GATTACCTGG TCAACATGGA 
TTTCCTGGTT TAAGAGGGAT TGCTGGCCTA GATGGTCTGC CAGGCAGCAA AGGAATCCAG 
GGACAACCAG GTGCAGAAAT ACCTGGTTTA AAGGGTTTTA AAGGTCTGAG TGGTGAAAAA 
GGTCAACCCG GAGAGTTTAG TACCATTGTA GGAATACCTG GACCTGCAGG ACCTAAAGGA 
ACCATAGGGG ATCAAGGTTT CCCTGGCTTA ACTGGACTTC CTGGAACAGT TGGAGTTCGT 
GGCTTGCCTG GGTTTTCTAA CAGGACAGGA ACGCTAGGAG CTGTTGGACA GCCAGGACCT 
CCTGGACTGC CAGGTTTTCC AGGCCCTGTG GGAACCCCAG GTTTACCCTC TAAACCTGGA 
GTAAAAGGAG AAATAGGAGA GCTGGGGCCA CAAGGGAACT ATGGCGCTAA AGGTATTCCT 
GGTGATCAAG GAAAAGTGGG AGCTCCTGGA GTTGCTGGAA TACCTGGAGT CAAAGGTTGG 
AAAGGCAATG CTGGCTTCCA AGGTGTGTCT GGGGTATTAG GGCATCTAGG TGATCAGGGA 
CTTAAAGGAT ATAAAGGTGA CAGAGGAGAA CACGGGTTAA AGGGTCCTTC TGGACAACCA 
GGCCCTATGT CTGAACCTCC TATTATTATT GCTGAACGTG GTTTCCATGG GCCACAAGGA 
ATTATTGGCC CTAAAGGTGT TCAGGGCGAA ATAGGTCCAA GAGGTGTTGT GGGTAGTCCA 
GGTGCCCCTG GCCAAACTGG GAAAGAAGGA TTACCAGGCA TACCAGGCTG GCCTGCACCT 
CCAGGCGGCC GAGGAGAATC GGGACCCATG GGACAAAGTG GACTTGATGG ACTAAAAGGT 
GCCACTGGTG TGCCAGGCAG GGCAGGGCAT CCTGGGATGC CGGGAAGAAG TATCAATATT 
GGCTATCTTT TAGTCAAGCA CAGTCAAACT GATGAAGAGC CAATGTGTCC TGTTGGTATG 
GCCCGGTTGT GGACTGGGTA CAGTTTGCTT TATTTTGAAG GACAGGAAAA GGCACACAAT 
CAGGACTTGG GTCTAGCTGG ATCTTGTTTG CAGCGCTTCA GTACAATGCC TTTCCTGTAC 
TGCAACCCTG GCGACGTCTG TTATTATGCA AACCGTAACG ATAAGTCTTA TTGGCTTTCC 
ACCACTGCTC CTCTTCCAAT GATGCCGGTG GTTGAAGAAG AAATAAGGCC ATATATTAGC 
CGTTGCTCAG TGTGCGAAGC TCCGGCAGTA GCAATCGCTG TTCATAGTCA AGATGTCAGT 
ATCCCTCATT GCCCAGATGG TTGGCGGAGC TTGTGGATTG GATACTCTTT TCTCATGCAC 
ACTGCAGCAG GTGACGAGGG AGGAGGACAA TCTCTCTCTT CCCCGGGAAG TTGTTTGGAA 
GACTTCAGGG CAACTCCATT CATTGAATGT AATGGCGAAA GAGGGACCTG CCACTACTTT 
GCAAACAAGT ACAGTTTCTG GCTTACTACT ATTGACCAGA CCTTCCAGAG CTCTCCGGCT 
GCAGATACTC TTAAAGCTGG GCTCATTCGA ACTCATATCA GCCGATGCCA AGTCTGCATG 
AAAAATCTGT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_018102693.1
CDS	185..5356
Translation	MTRTQSTKRCATSSKEHNHFLLKHHSVTWYVMFVVLVTLFAGNAHAGDKSYTGPCGGRDCSQGCQCLPEKGSRGQPGPLGPQGTPGPPGLIGIAGLQGRKGDKGERGFPGVTGPFGDKGQRGVTGFSGADGIPGHTGQGGSRGKPGHDGCNGTKGDAGDGGSHGPRGPPGILGGFGVKGQKGEPFHVSIEVKNRLRGDPGEPGFNGIQGPRGSPGIAGFIGPQGYRGPRGPPGPPGTQGLQGNRGLGYYGEKGDTGDPGPPGEQIPPGEREQQHKTEILLLKYKGAKGDQGERGIRGDKGQQAVTGEKGEEGITGFPGQRGLPGNDGGRGNAGEIGFPGSNGAPGLPGYKGIKGEHGNRGPAGPVTFLKGQKPKKGYEGDMGFPGLNGLYGDDGDLGDPGFPGQNGFPSTFPGRTGDPGLNGQRGPKGYQGDPGLPAFQPGPPGKDGEPGNEGPPGKPGYPGGRIGMKGLPGPPGRDGHRGFPGSRGFKGIKGEQGECKCFAGDEAGRGPAGESGPVGFPGFQGLSGRKGEAGDRGVQGLPGTPGTVGLTGPAGFPGPAGEKGDKVVATEKGSKGVQGNSGFPGAPGRDGYPGRNGRDGIPGFQGPPGDGVKGLPGYAGFPGQPGTPGVLGETGFPGPGFPGPKGVRGEPGNRGISGFPGIPGLSGPQGDCYQSADEENEIGKEGTGCHLIAPPAPEGARGPPGLPGYNGLRGQKGFPGPQGFNGVFGLKGVKGEPGNDSLPGPPGFPGQRGDRGNAGIPGLPGAPGLSGKPGFKGVQGEKGGAGDRLGAPPGEQGNTGLPGLQGLKGLQGDQGIPGLQGKVGIPGTPGNKGSKGSAGAPGGPGIPGPAAQFGFPGMPGNPGTPGPHGSMGPPGSPGPRGDEGDKGPPGPVGMKGLPGELGNEGFVGIRGKQGAPGIPGLVGMHGLPGVKGVKGSPGMEGFHGMKGAKGRPAWRGPKGQSGNIGFPGVKGRDGALGYKGDRGERGLPGEPPEMLPEVMHLAKGEKGDQGVPGFKGTTGPTGSKGMPGDPGQPGLSGLPGIPSFEFGDKGDTGLPGRGGHPGIPGEVGPRGIIGFPGFTGPRGTPGFSGLPGVPGERGHYGDTGQRGDTIDLPGERGVKGQTGSPGLSGQHGIHGEKGIKGEAGSYGIEGMRGEPGQPGEKGFPGQQGLLGLQGSQGSPGQPGVHGEKGNTGFPGLPGQHGFPGLRGIAGLDGLPGSKGIQGQPGAEIPGLKGFKGLSGEKGQPGEFSTIVGIPGPAGPKGTIGDQGFPGLTGLPGTVGVRGLPGFSNRTGTLGAVGQPGPPGLPGFPGPVGTPGLPSKPGVKGEIGELGPQGNYGAKGIPGDQGKVGAPGVAGIPGVKGWKGNAGFQGVSGVLGHLGDQGLKGYKGDRGEHGLKGPSGQPGPMSEPPIIIAERGFHGPQGIIGPKGVQGEIGPRGVVGSPGAPGQTGKEGLPGIPGWPAPPGGRGESGPMGQSGLDGLKGATGVPGRAGHPGMPGRSINIGYLLVKHSQTDEEPMCPVGMARLWTGYSLLYFEGQEKAHNQDLGLAGSCLQRFSTMPFLYCNPGDVCYYANRNDKSYWLSTTAPLPMMPVVEEEIRPYISRCSVCEAPAVAIAVHSQDVSIPHCPDGWRSLWIGYSFLMHTAAGDEGGGQSLSSPGSCLEDFRATPFIECNGERGTCHYFANKYSFWLTTIDQTFQSSPAADTLKAGLIRTHISRCQVCMKNL

Target ORF information:

RefSeq Version	XM_018247204.1
Organism	Xenopus laevis(African clawed frog)
Definition	Xenopus laevis collagen, type IV, alpha 2 L homeolog (col4a2.L), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_018247204.1

ORF Insert Sequence:

ATGACAAGAA CTCAAAGCAC CAAAAGATGT GCCACAAGCA GCAAAGAGCA TAACCATTTT 
CTACTCAAAC ATCATTCAGT CACTTGGTAC GTGATGTTCG TTGTACTGGT GACACTTTTT 
GCTGGAAATG CCCATGCTGG TGACAAGTCA TATACAGGGC CCTGTGGAGG AAGAGACTGC 
AGTCAGGGGT GCCAGTGTCT GCCGGAGAAA GGAAGTCGCG GACAACCAGG ACCTCTTGGC 
CCGCAAGGTA CCCCTGGCCC ACCAGGACTC ATAGGAATAG CAGGTCTCCA GGGACGTAAG 
GGAGACAAAG GAGAGCGAGG ATTTCCTGGA GTGACTGGAC CCTTTGGAGA CAAGGGCCAA 
AGAGGTGTTA CAGGTTTTTC CGGAGCTGAT GGCATACCTG GACATACAGG TCAGGGTGGC 
TCAAGAGGTA AACCAGGTCA TGATGGTTGT AATGGAACAA AAGGTGATGC TGGTGATGGG 
GGCTCACATG GTCCAAGAGG CCCTCCTGGA ATTCTTGGTG GATTTGGTGT CAAAGGCCAA 
AAAGGAGAAC CATTTCATGT ATCCATTGAG GTGAAAAACA GACTTAGAGG AGATCCTGGT 
GAACCAGGCT TTAATGGCAT TCAGGGTCCA AGGGGTTCTC CTGGAATAGC AGGATTCATT 
GGGCCACAGG GTTATCGTGG CCCTCGTGGG CCACCTGGAC CTCCTGGGAC ACAAGGACTA 
CAAGGCAACA GAGGACTTGG CTATTACGGA GAAAAAGGAG ACACTGGTGA TCCAGGTCCA 
CCTGGTGAAC AAATTCCTCC AGGAGAGAGA GAACAACAGC ACAAAACTGA AATTCTCTTG 
CTAAAATATA AGGGGGCCAA GGGGGACCAA GGAGAAAGGG GAATTCGAGG TGATAAAGGT 
CAACAAGCTG TCACAGGAGA AAAAGGAGAA GAAGGAATCA CAGGATTTCC TGGACAAAGG 
GGATTGCCTG GTAATGATGG TGGCCGTGGA AATGCAGGAG AAATAGGGTT TCCTGGGTCA 
AATGGAGCAC CTGGATTACC TGGTTATAAG GGAATAAAGG GAGAACATGG GAACCGAGGA 
CCTGCAGGAC CTGTTACCTT TCTCAAAGGT CAAAAACCAA AAAAAGGTTA TGAAGGTGAT 
ATGGGATTTC CAGGACTTAA TGGGCTTTAT GGAGATGACG GAGACTTGGG AGATCCTGGT 
TTTCCAGGTC AGAATGGATT TCCAAGTACA TTTCCAGGAC GCACAGGTGA TCCAGGATTG 
AATGGTCAAA GGGGCCCCAA AGGTTACCAA GGTGATCCAG GCTTACCAGC ATTTCAGCCA 
GGACCCCCGG GTAAAGATGG AGAACCTGGC AATGAAGGAC CACCTGGCAA ACCTGGATAC 
CCAGGTGGTC GCATTGGGAT GAAAGGATTA CCTGGGCCTC CAGGAAGGGA TGGACATAGA 
GGATTTCCTG GTTCTCGGGG TTTCAAAGGA ATTAAAGGTG AACAAGGGGA ATGCAAGTGT 
TTTGCAGGAG ATGAAGCTGG TAGAGGCCCT GCTGGAGAGT CTGGTCCAGT GGGTTTCCCA 
GGTTTTCAGG GTCTGTCAGG CAGAAAAGGA GAAGCGGGAG ATAGAGGAGT ACAAGGGCTA 
CCAGGAACAC CAGGCACAGT GGGTCTGACT GGTCCTGCTG GGTTTCCAGG TCCTGCTGGA 
GAGAAGGGTG ATAAAGTAGT TGCTACAGAA AAAGGTTCCA AGGGAGTGCA AGGCAACTCT 
GGTTTTCCTG GTGCCCCTGG TCGTGATGGA TACCCGGGAA GAAATGGTAG AGATGGTATT 
CCTGGCTTTC AAGGCCCTCC TGGAGATGGT GTAAAAGGAC TCCCGGGTTA TGCTGGGTTT 
CCTGGACAAC CTGGTACTCC TGGTGTTCTA GGTGAGACAG GCTTTCCTGG ACCTGGATTT 
CCAGGTCCAA AAGGTGTACG TGGAGAACCA GGAAACAGAG GCATTAGTGG ATTCCCAGGA 
ATTCCTGGAC TTTCAGGTCC CCAAGGTGAC TGCTACCAAT CAGCAGATGA AGAAAATGAA 
ATAGGGAAAG AAGGAACTGG CTGCCATCTG ATTGCACCAC CTGCTCCAGA GGGCGCCCGA 
GGGCCACCAG GATTGCCAGG ATATAACGGT TTAAGAGGCC AGAAAGGTTT CCCTGGACCA 
CAAGGTTTTA ATGGAGTGTT TGGATTAAAA GGAGTCAAAG GAGAACCTGG GAATGATAGT 
TTGCCCGGTC CACCAGGTTT TCCTGGGCAA AGGGGAGATA GAGGTAATGC AGGAATTCCT 
GGTCTACCAG GGGCACCGGG TCTATCAGGA AAACCTGGCT TTAAAGGGGT TCAAGGAGAA 
AAGGGAGGTG CAGGTGATCG CTTAGGAGCT CCACCAGGGG AGCAAGGAAA TACTGGACTG 
CCTGGGTTGC AAGGACTGAA AGGATTACAA GGGGATCAAG GCATACCTGG TCTGCAAGGT 
AAAGTTGGAA TACCAGGAAC ACCTGGAAAC AAGGGTAGTA AAGGCAGTGC TGGGGCCCCA 
GGAGGACCTG GAATACCAGG ACCTGCAGCA CAATTTGGAT TTCCAGGAAT GCCAGGAAAC 
CCAGGCACTC CAGGACCCCA CGGTTCAATG GGACCTCCAG GTTCTCCAGG ACCGAGAGGA 
GATGAAGGCG ATAAAGGGCC ACCAGGTCCG GTTGGCATGA AAGGGTTACC TGGAGAACTA 
GGCAATGAAG GATTTGTTGG AATTAGGGGG AAACAAGGGG CTCCAGGTAT CCCTGGCTTG 
GTTGGAATGC ATGGACTTCC TGGTGTGAAA GGTGTCAAAG GTTCCCCTGG CATGGAAGGA 
TTTCATGGAA TGAAAGGAGC AAAGGGGAGA CCTGCATGGA GAGGACCCAA GGGACAAAGT 
GGCAATATAG GATTTCCAGG AGTAAAAGGG AGAGACGGAG CACTGGGTTA CAAAGGTGAC 
CGTGGGGAAA GGGGACTACC AGGAGAGCCC CCCGAAATGC TGCCAGAAGT TATGCATTTG 
GCAAAAGGTG AAAAGGGAGA TCAAGGTGTT CCTGGATTCA AGGGCACTAC TGGACCTACA 
GGCAGTAAAG GTATGCCAGG AGATCCTGGA CAGCCAGGGT TATCTGGTTT GCCTGGCATA 
CCAAGCTTTG AATTTGGTGA TAAAGGAGAT ACTGGCTTGC CTGGGCGTGG TGGTCATCCA 
GGAATTCCAG GAGAAGTTGG TCCACGAGGA ATTATTGGCT TCCCAGGATT TACTGGTCCT 
AGGGGAACTC CTGGTTTCTC TGGCCTTCCT GGTGTTCCTG GTGAAAGAGG CCATTATGGA 
GATACAGGCC AGAGAGGTGA TACTATTGAT TTACCAGGAG AAAGAGGAGT TAAGGGTCAG 
ACTGGAAGCC CTGGGCTGTC AGGACAACAT GGAATACATG GAGAAAAAGG AATAAAAGGG 
GAAGCCGGTT CCTATGGTAT TGAGGGAATG AGAGGTGAAC CTGGACAACC AGGAGAAAAG 
GGATTCCCAG GTCAGCAAGG CCTGTTGGGC TTACAAGGCT CACAGGGTTC ACCGGGTCAA 
CCGGGTGTAC ATGGCGAGAA AGGAAATACT GGGTTTCCTG GATTACCTGG TCAACATGGA 
TTTCCTGGTT TAAGAGGGAT TGCTGGCCTA GATGGTCTGC CAGGCAGCAA AGGAATCCAG 
GGACAACCAG GTGCAGAAAT ACCTGGTTTA AAGGGTTTTA AAGGTCTGAG TGGTGAAAAA 
GGTCAACCCG GAGAGTTTAG TACCATTGTA GGAATACCTG GACCTGCAGG ACCTAAAGGA 
ACCATAGGGG ATCAAGGTTT CCCTGGCTTA ACTGGACTTC CTGGAACAGT TGGAGTTCGT 
GGCTTGCCTG GGTTTTCTAA CAGGACAGGA ACGCTAGGAG CTGTTGGACA GCCAGGACCT 
CCTGGACTGC CAGGTTTTCC AGGCCCTGTG GGAACCCCAG GTTTACCCTC TAAACCTGGA 
GTAAAAGGAG AAATAGGAGA GCTGGGGCCA CAAGGGAACT ATGGCGCTAA AGGTATTCCT 
GGTGATCAAG GAAAAGTGGG AGCTCCTGGA GTTGCTGGAA TACCTGGAGT CAAAGGTTGG 
AAAGGCAATG CTGGCTTCCA AGGTGTGTCT GGGGTATTAG GGCATCTAGG TGATCAGGGA 
CTTAAAGGAT ATAAAGGTGA CAGAGGAGAA CACGGGTTAA AGGGTCCTTC TGGACAACCA 
GGCCCTATGT CTGAACCTCC TATTATTATT GCTGAACGTG GTTTCCATGG GCCACAAGGA 
ATTATTGGCC CTAAAGGTGT TCAGGGCGAA ATAGGTCCAA GAGGTGTTGT GGGTAGTCCA 
GGTGCCCCTG GCCAAACTGG GAAAGAAGGA TTACCAGGCA TACCAGGCTG GCCTGCACCT 
CCAGGCGGCC GAGGAGAATC GGGACCCATG GGACAAAGTG GACTTGATGG ACTAAAAGGT 
GCCACTGGTG TGCCAGGCAG GGCAGGGCAT CCTGGGATGC CGGGAAGAAG TATCAATATT 
GGCTATCTTT TAGTCAAGCA CAGTCAAACT GATGAAGAGC CAATGTGTCC TGTTGGTATG 
GCCCGGTTGT GGACTGGGTA CAGTTTGCTT TATTTTGAAG GACAGGAAAA GGCACACAAT 
CAGGACTTGG GTCTAGCTGG ATCTTGTTTG CAGCGCTTCA GTACAATGCC TTTCCTGTAC 
TGCAACCCTG GCGACGTCTG TTATTATGCA AACCGTAACG ATAAGTCTTA TTGGCTTTCC 
ACCACTGCTC CTCTTCCAAT GATGCCGGTG GTTGAAGAAG AAATAAGGCC ATATATTAGC 
CGTTGCTCAG TGTGCGAAGC TCCGGCAGTA GCAATCGCTG TTCATAGTCA AGATGTCAGT 
ATCCCTCATT GCCCAGATGG TTGGCGGAGC TTGTGGATTG GATACTCTTT TCTCATGCAC 
ACTGCAGCAG GTGACGAGGG AGGAGGACAA TCTCTCTCTT CCCCGGGAAG TTGTTTGGAA 
GACTTCAGGG CAACTCCATT CATTGAATGT AATGGCGAAA GAGGGACCTG CCACTACTTT 
GCAAACAAGT ACAGTTTCTG GCTTACTACT ATTGACCAGA CCTTCCAGAG CTCTCCGGCT 
GCAGATACTC TTAAAGCTGG GCTCATTCGA ACTCATATCA GCCGATGCCA AGTCTGCATG 
AAAAATCTGT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

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col4a2.L cDNA ORF clone, Xenopus laevis(African clawed frog)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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