LOC108716059 cDNA ORF clone, Xenopus laevis(African clawed frog)

Gene
Clones
gRNAs

Summary

Gene Symbol	LOC108716059
Entrez Gene ID	108716059
Full Name	carbamoyl-phosphate synthetase 2, aspartate transcarbamylase, and dihydroorotase L homeolog
Synonyms	viar02,xcad
General protein information	Preferred Names carbamoyl-phosphate synthetase 2, aspartate transcarbamylase, and dihydroorotase L homeolog Names CAD protein-like
Gene Type	protein-coding
Organism	Xenopus laevis(African clawed frog)

mRNA and Protein(s)

mRNA	Protein	Name
XM_018262173.1	XP_018117662.1	CAD protein-like isoform X1
XM_018262174.1	XP_018117663.1	CAD protein-like isoform X2

Pathways from BioSystems
Gene Ontology

The following LOC108716059 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC108716059 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OAf31605	XM_018262173.1 Latest version!	Xenopus laevis CAD protein-like (LOC108716059), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OAf31606	XM_018262174.1 Latest version!	Xenopus laevis CAD protein-like (LOC108716059), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LOC108716059 ( XM_018262173.1 ) cDNA ORF clone, Xenopus laevis(African clawed frog) -> XP_018117662.1 Xenopus laevis CAD protein-like (LOC108716059), transcript variant X1, mRNA.

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CloneID	OAf31605
Clone ID Related Accession (Same CDS sequence)	XM_018262173.1
Accession Version	XM_018262173.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6744bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1474214400000
Organism	Xenopus laevis(African clawed frog)
Product	CAD protein-like isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030732.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus laevis Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTGCCG CTGCGTTTCT GCTGCTCGAG GATGGGACTC GCCTCCCCGG GAGGATGTTC 
GGGGCTCAGC GCTTTGTCTC CGGGGAAGTG GTGTTCCAGA CGGGCATGGT GGGCTACCCC 
GAGTCCCTGA CCGACCCCTC ATACAAGAGT CAGATCCTGG TGCTGACGTA TCCGCTCATA 
GGGAACTATG GGATCCCCCG CGATCAACTC GACCAGTTTG GGCTCAGCCG GTGGTTTGAG 
TCCAGTAAGA TTCATGTCTC GGCGCTGATT ATTGGGGAGC AATCGCAGAA CCCCAGTCAC 
TGGAGCTGTG ACATCTCTTT GAACCAGTGG CTGAAGCAGC AGGGAATCCC CGGACTGGAA 
GGTGTGGACA CCCGGGCGCT GACCAAGAAG ATCCGAGAGA AGGGCACCCT GCTGGGCAAG 
CTCATAGCCC CCGGCACCGA CGAGACACAA GTCCCTTATG AAGATCCCGG GAAGAGGAAC 
CTGGTGCAGG AGGTGTCACT GAAGGAGCCC CGAGTGTTCA ATTCTGGGGG TGCAGTGAGG 
GTCGCTGCCC TGGACTGTGG GCTGAAGTTC AACCAGATCC GCTGCCTGTG CCAGAGGGGG 
GCCGAGGTGA CGGTGCTGCC ATGGGATTAT CCAATCAGCG CTGCAGAGTT TGACGGACTG 
TTCCTCAGTA ACGGCCCCGG GGACCCCACT TTCTGCCAGA AATCCGTGCA GAACATCAGG 
GGGTTCATCT CACAGGAGAA GCCAAAACCC GTATTTGGGA TCTGTCTCGG CCACCAGATC 
CTCTCCCTGG TTATTGGAGC CAAGACCTAC AAGATGAAGT ACGGGAATCG TGGGCACAAC 
CAGCCGTGTA TACACGAGGG CTCTCAGCGC TGCTATATAA CCTCCCAGAA CCACGGATAC 
GCTGTGGACC CAAACTCCTT ACCTGAAGGC TGGTCTCCTC TGTTCACTAA TGCCAACGAC 
CAATCAAACG AGGGGATTAT ACATGACTCC AAACCCTTCT TCAGTGTGCA GTTCCACCCG 
GAGCACAGTG CTGGGCCCAT GGACCTGGTG TGTCTATTTG ATGTATTTCT GGAGACTGTG 
AGAGACATAA AGGCCGGGAA TTCGGGAGAG AAGTCAGTAA AACAGCGACT GACTGAGCTG 
CTCACGTACA AAGACTCCCC CGGTGTCACA GACTTGGAGG CCTCTCGTCC GCAAAAAGTG 
CTGATCCTGG GCTCAGGGGG GCTCTCGATA GGCCAGGCCG GGGAGTTCGA CTACTCTGGC 
TCTCAGGCCA TTAAAGCACT GAAGGAAGAG AACATCCAGA CAGTCCTGAT TAACCCCAAC 
ATTGCCACAG TACAGACATC AAAAGGCCTG GCCGACAAGG TTTATTTCCT GCCAATCACC 
CCTGAATATG TCACTCAGGT GATAAAGAAT GAGCGGCCAG ATGGAATACT CCTGACGTTT 
GGGGGTCAGA CTGCATTGAA TTGTGGGGTG GAGCTGACCA AGCAGGGGGT CCTAGAAAAG 
TACCATGTGC GTGTTCTGGG GACCCCTGTG AAATCCATTG AAATGACAGA AGATCGCAAA 
GTGTTTGTGG AGAAGATGGA GGAGATTGGA GAACACGTGG CCCCCAGTGA GGCGGCTTTC 
TCCTTGGAGC AGGCCCAGGC TGCTGCAGAA CGTCTCGGGT ATCCAGTTTT GGTCCGGTCA 
GCTTATGCAC TGGGTGGGCT AGGATCTGGA TTTGCCAATA ACAAGGAAGA GCTGGCTGCC 
CTGGTCTGCC AGGCCTTCGC ACACACAACG CAGGTCCTGG TGGATAAATC CCTGAAGGGC 
TGGAAGGAGA TTGAGTATGA AGTCGTGCGG GACGCCTATG ATAACTGTGT AACGGTTTGC 
AACATGGAGA ACGTGGATCC CCTTGGCATT CACACTGGAG AATCCATCGT GGTAGCTCCC 
AGCCAGACGC TGAACGATCG AGAGTACAAC ATGCTGCGCA ACACGGCCAT TAAGGTTATC 
CAGCACCTCG GCATTGTGGG CGAGTGCAAC ATACAGTACG CCTTAAATCC AGAATCTGAG 
CAGTATTATA TTATTGAGGT CAACGCAAGA CTGTCTCGTA GCTCAGCCCT GGCGAGTAAA 
GCAACCGGTT ACCCCCTGGC GTATGTGGCT GCCAAGCTGG CACTGGGAAT TCCACTGCCA 
GTTCTCAGGA ACTCTGTAAC CAACTCCACC ACGGCTAACT TTGAGCCCAG CCTGGATTAC 
TGTGTGGTGA AGGTTCCACG CTGGGACCTG AGCAAGTTCT CGCGAGTCAG TACCAAGATT 
GGAAGCTCCA TGAAGAGTGT GGGCGAGGTC ATGTCTGTTG GTAGGAGCTT TGAAGAAGCC 
TTCCAGAAAG CTCTGCGCAT GGTCGATGAG AACTGTGTGG GCTTCGATCA CTGCATCAGA 
TCTGCATCTG ATGAGGAACT GGAGACACCA ACAGATAAGC GAATCTTCGT GCTGGCCGCA 
GCCCTGAGAG TTGGTTACAC CATCGAGCGC CTCTATAAGT TGACCAGGAT TGACAAGTGG 
TTCCTCCATA AGATGAAGAA CATTACTGAT CACTTGGCCA CACTGGAGTC TTATCACCAG 
GATGAGACCT TGATGCCCCG TGAGGTCCTT CTCCGAGCCA AACAGCTGGG CTTCTCCGAT 
AAGCAGATTG CACTGGCCGT GCAAAGCACG GAGCTGGCAG TCCGGAAGAT GAGGCAGGAT 
CTGCAGATTT TGCCTGTAGT GAAGCAGATC GACACGGTAG CAGCGGAGTG GCCGGCTCAG 
ACCAATTACC TTTATCTGAC CTACAATGGC ATTGAGCATG ATTTGACCTT TCATGAGCCG 
CATGTTATGG TCATTGGCTC TGGTGTTTAC CGAATAGGTA GCAGCGTGGA GTTTGATTGG 
TGTGCAGTGG GCTGCATCCA GGAGCTGCGC AAGATGGGAT TTAAGACCAT CATGGTGAAT 
TACAATCCCG AGACTGTCAG CACAGACTAC GACATGTGTG ACCGCCTGTA TTTTGATGAG 
ATCTCGTTTG AGGTGGTGAT GGATATCTAT GAGCTTGAGA ACCCAGAGGG AATCATACTG 
TCCATGGGCG GCCAGCTACC CAATAACATG GCCATGTCCC TACATCGCCA GCAGTGCCGG 
GTACTGGGCA CCTCTCCTGA GGACATTGAC TCCGCTGAGA ACAGATTTAA GTTTTCTCGC 
CTTTTGGACA CAATTGGTAT TAGTCAGCCG CAGTGGAAAG AGCTGTCTGA CACGGAGTCA 
GCCGTTCAGT TCTGCAACAC TGTCGGCTTC CCCTGTGTTG TGCGTCCATC CTATGTGCTG 
AGCGGAGCCG CTATGAATGT GGCTTATTCA GATGAAGACA TGGAGAAGGT GCTTAGCAAT 
GCCGTGGCTG TATCCAAGGA GCATCCTGTG GTCATCTCTA AATTCATCCA GGAGGCCAAG 
GAAATTGATG TGGATGCCGT GGCCTGTGAT GGAGTTGTGG TTGCCGTGGC TATTTCGGAA 
CATGTGGAGA ATGCAGGGGT ACACTCTGGG GATGCAACAC TGGTGACCCC GGCTCAGGAT 
ATCACCCCCA AAACCTTGGA AAGGATTAAA GCCATTGTCC ACGCTGTGGG GCTGGAGCTC 
CAAGTCACCG GGCCCTTCAA CCTGCAACTC ATTGCTAAGG ATGACCAGCT GAAAGTGATT 
GAGTGCAACG TTCGTGTGTC TCGCTCCTTT CCCTTTGTGT CTAAGACTCT GGGGGTTGAT 
CTAGTAGCTC TGGCAACGCA AGTCATTATG GGAGAAGAGG TTGAACCTGT TGGTCTGATG 
ACTGGTACTG GCATTGTAGG GGTTAAGGTT CCACAGTTCT CGTTCTCACG CTTGGCTGGA 
GCAGACGTGG TCCTGGGGGT GGAGATGACC AGCACAGGGG AGGTGGCCTG CTTTGGAGAG 
AACCGATATG AGGCTTATCT AAAAGCCATG CTAAGTACTG GGTTCAAAAT CCCACAGAAG 
AACATCCTGC TGACTATTGG GAGCTATAAG AACAAGAGCG AGCTCCTGCC CACTGTGCGA 
GCCATGGAAA GCCTTGGGTA CAATCTGTAT GCCAGCCTGG GAACTGCTGA CTTCTACACC 
GAGCATGGCA TCAAGGTAAA GGCAATGGAC TGGCCCTTTG AGGAGAGCGA GAATGATGGG 
CCCCTACGGG AGAAGCAGGG GAGCATCATG GACCATTTGT CAGAGAATCA CTTTGATTTG 
GTCATTAACC TGTCCATGCG GAATTCTGGG GGTCGCCGAC TTTCCTCCTT TGTTACCCGT 
GGGTATCGTG CCCGCCGATT GGCTGTAGAC TATTCCGTGC CCCTCATCAT CGATATTAAG 
TGTACCAAAC TCTTTGTGGA GGCTCTGCGG CAGATTGGAC GGTCGCCCCC TGTTAAGACT 
CACGTTGATT GTATGACCTC ACAGAAGTTT ATCAGGCTGC CGGGCCTCAT TGATATCCAT 
GTGCACCTAC GGGAACCTGG AGCCACTCAT AAAGAGGACT TTTCTTCTGG CACAGCGGCT 
GCACTGGCTG GTGGAGTCAC CCTTGTATGT GCCATGCCGA ACACCAATCC AGCCATCACT 
GATGCCAGTT CCTTCTCTCT GGCACAGAAG CTGGCGGAGG CTGGTGCCCG CTGTGACTTT 
GCCCTTTTCC TTGGTGCTTC CTCAGAGAAT GCCGCTTCTC TTTCTCCAAT TGCCAGCTCA 
GCTGCTGGCC TTAAGATGTA TCTGAATGAC ACCTACTCTC AGCTTAAGAT GGACAATGTT 
TCTGTGTGGA TGGAGCACTT TGAGAAGTGG CCGGCTCACC TGCCCATTGT GGCACATGCA 
GAGAAACAGA CCGTGGCTGC GATTCTCATG GTGGCCCAAC TGTACCAGCG TCCTGTTCAC 
ATTTGCCACG TAGCAAAGAA AGAGGAGATC CTAATTATTA AAGCCGCCAA GCAGAAGGGA 
ATCAAAGTAA CATGTGAGGT GGCCCCACAT CACCTTTTCC TGTGCCTCAA TGACTTGGAG 
AAAATAGGAG AGGCACGGGG GCAAGTACGC CCCATGCTGG GGACTCGAGA GGACCTAGAA 
TCTCTGTGGG AAAACTTGGA TATTATAGAC TGTTTTGCCA CTGACCACGC CCCTCACTCT 
GTGGAGGAAA AGGATGGCCC AAACCCACCC CCTGGGTATC CAGGCTTGGA GACCATGTTG 
CCTCTGCTCC TCACAGCAGT CAGTGAGGGA CGTCTGACCA TTGATGACAT CATTAAGCGT 
CTGTATGACA ACCCCCGCAA GATCTTCTCC TTGCCTGTGC AGGAGAACAC TTATGTAGAG 
GTGGACTTGG AGCAAGAGTG GGTAATCCCA TCCCACATGC AGTTTACCAG GTCCAGGTGG 
ACCCCCTTTG CTGGAATGAA GGTGAAAGGA GCCGTGCGTC GAGTTGTGCT GCGTGGGGAG 
GTGGCCTACA TTGATGGCCA GGTGTTGGTA CCTGCAGGAT ATGGACAGGA TGTCAGGAAA 
TGGACCCCAC CCGTTGTTGC CCAATCTCAC CCCATGGTCA ATAAAGAGAT ACCCAAGACC 
CCAGAGCGGC ACCGCCATGT TACAGTACAA AGTGAGACCA TTCGCAGTCG AGCACCAAGC 
CCCCGCAGAC CTGTTCAGTC GGCAGATGGG CGCTTCTACT TGCCTCCTCG GGTGCATCGT 
GCTTCAGATC CTGGGTTACC TGCAGAGGAG CAGAAAGAGA AGGCACCCAA GAGATCCGTG 
GAGCCAGAGC TGGTGCCCAT CCAGGATGGC TATTCCCACC CTCCTCCTAT TCCTAGAATT 
ATGTCCCCCC AGAGTACGTC CATGCAAAGT GTCCCTCATG TGCAGACCTC ACCCCTGTTG 
CACCCACTGA TTGGCCAGCA CATCCTGACG GTTAAGCAGT TCAGCAAAGA CCAGATGTCC 
CACCTGTTTA ACGTGGCACA CACGCTGCGC ATATTGGTTC AGAAGGAGAG AAACCTAGAT 
ATGCTGAAGG GCAAGGTGAT GGCCTCCATG TTTTTTGAGG CCAGCACACG TACAAGCAGC 
TCCTTTGCTG CAGCGATGCA ACGTCTTGGG GGCTCTGTGC TCAGTTTCTG TGAGACAACC 
TCCTCTTCTC AAAAAGGAGA ATCACTCATT GACTCTGTTC AGACAATGAG CTGCTATTCC 
GATGTCCTTG TGGTGAGACA CCCAGAGCCT GGAGCTGTGG AGCTTGCTGC CAAGCACTGT 
CGGAAGCCTG TGATAAATGC TGGGGATGGA GTGGGCGAGC ACCCCACACA GGCACTACTG 
GACATTTTCA CAATCCGTGA AGAGCTGGGC ACAGTCAATG GAATGATAAT AACAATGGTC 
GGCGATTTGA AACATGGCAG GACAGTTCAC TCCCTCGCCT GCCTTCTAAC GCAGTACCGC 
GTGACTTTGA GATACGTGAC TCCCCGCAGC CTTCGTATGC CCTCCAACAT CATACACTTT 
GTAGCCTCCA AAGGCATTAA GCAGGAAGAG TTTGAGAGCA TTGAAGAAGC TCTGCCGGAT 
ACAGATGTGC TGTATATGAC CCGAATACAG AAGGAGAGAT TTAATTCTTC CGACGAGTAC 
AATGCTTGTT TTGGCCAATT TATCCTGACC CCACACATCA TGACCAGAGC CAAGAAGAGG 
ATGGTGGTAA TGCATCCGAT GCCTCGTGTG AACGAGATCA GTTTTGAAGT GGATTCCGAC 
CCCCGGGCAG CTTACTTCCG CCAGGCTGAG AACGGCATGT ATATCCGCAT GGCTCTCTTA 
GCAACAGTTT TGGGAAAATA CTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_018117662.1
CDS	152..6895
Translation	MAAAAFLLLEDGTRLPGRMFGAQRFVSGEVVFQTGMVGYPESLTDPSYKSQILVLTYPLIGNYGIPRDQLDQFGLSRWFESSKIHVSALIIGEQSQNPSHWSCDISLNQWLKQQGIPGLEGVDTRALTKKIREKGTLLGKLIAPGTDETQVPYEDPGKRNLVQEVSLKEPRVFNSGGAVRVAALDCGLKFNQIRCLCQRGAEVTVLPWDYPISAAEFDGLFLSNGPGDPTFCQKSVQNIRGFISQEKPKPVFGICLGHQILSLVIGAKTYKMKYGNRGHNQPCIHEGSQRCYITSQNHGYAVDPNSLPEGWSPLFTNANDQSNEGIIHDSKPFFSVQFHPEHSAGPMDLVCLFDVFLETVRDIKAGNSGEKSVKQRLTELLTYKDSPGVTDLEASRPQKVLILGSGGLSIGQAGEFDYSGSQAIKALKEENIQTVLINPNIATVQTSKGLADKVYFLPITPEYVTQVIKNERPDGILLTFGGQTALNCGVELTKQGVLEKYHVRVLGTPVKSIEMTEDRKVFVEKMEEIGEHVAPSEAAFSLEQAQAAAERLGYPVLVRSAYALGGLGSGFANNKEELAALVCQAFAHTTQVLVDKSLKGWKEIEYEVVRDAYDNCVTVCNMENVDPLGIHTGESIVVAPSQTLNDREYNMLRNTAIKVIQHLGIVGECNIQYALNPESEQYYIIEVNARLSRSSALASKATGYPLAYVAAKLALGIPLPVLRNSVTNSTTANFEPSLDYCVVKVPRWDLSKFSRVSTKIGSSMKSVGEVMSVGRSFEEAFQKALRMVDENCVGFDHCIRSASDEELETPTDKRIFVLAAALRVGYTIERLYKLTRIDKWFLHKMKNITDHLATLESYHQDETLMPREVLLRAKQLGFSDKQIALAVQSTELAVRKMRQDLQILPVVKQIDTVAAEWPAQTNYLYLTYNGIEHDLTFHEPHVMVIGSGVYRIGSSVEFDWCAVGCIQELRKMGFKTIMVNYNPETVSTDYDMCDRLYFDEISFEVVMDIYELENPEGIILSMGGQLPNNMAMSLHRQQCRVLGTSPEDIDSAENRFKFSRLLDTIGISQPQWKELSDTESAVQFCNTVGFPCVVRPSYVLSGAAMNVAYSDEDMEKVLSNAVAVSKEHPVVISKFIQEAKEIDVDAVACDGVVVAVAISEHVENAGVHSGDATLVTPAQDITPKTLERIKAIVHAVGLELQVTGPFNLQLIAKDDQLKVIECNVRVSRSFPFVSKTLGVDLVALATQVIMGEEVEPVGLMTGTGIVGVKVPQFSFSRLAGADVVLGVEMTSTGEVACFGENRYEAYLKAMLSTGFKIPQKNILLTIGSYKNKSELLPTVRAMESLGYNLYASLGTADFYTEHGIKVKAMDWPFEESENDGPLREKQGSIMDHLSENHFDLVINLSMRNSGGRRLSSFVTRGYRARRLAVDYSVPLIIDIKCTKLFVEALRQIGRSPPVKTHVDCMTSQKFIRLPGLIDIHVHLREPGATHKEDFSSGTAAALAGGVTLVCAMPNTNPAITDASSFSLAQKLAEAGARCDFALFLGASSENAASLSPIASSAAGLKMYLNDTYSQLKMDNVSVWMEHFEKWPAHLPIVAHAEKQTVAAILMVAQLYQRPVHICHVAKKEEILIIKAAKQKGIKVTCEVAPHHLFLCLNDLEKIGEARGQVRPMLGTREDLESLWENLDIIDCFATDHAPHSVEEKDGPNPPPGYPGLETMLPLLLTAVSEGRLTIDDIIKRLYDNPRKIFSLPVQENTYVEVDLEQEWVIPSHMQFTRSRWTPFAGMKVKGAVRRVVLRGEVAYIDGQVLVPAGYGQDVRKWTPPVVAQSHPMVNKEIPKTPERHRHVTVQSETIRSRAPSPRRPVQSADGRFYLPPRVHRASDPGLPAEEQKEKAPKRSVEPELVPIQDGYSHPPPIPRIMSPQSTSMQSVPHVQTSPLLHPLIGQHILTVKQFSKDQMSHLFNVAHTLRILVQKERNLDMLKGKVMASMFFEASTRTSSSFAAAMQRLGGSVLSFCETTSSSQKGESLIDSVQTMSCYSDVLVVRHPEPGAVELAAKHCRKPVINAGDGVGEHPTQALLDIFTIREELGTVNGMIITMVGDLKHGRTVHSLACLLTQYRVTLRYVTPRSLRMPSNIIHFVASKGIKQEEFESIEEALPDTDVLYMTRIQKERFNSSDEYNACFGQFILTPHIMTRAKKRMVVMHPMPRVNEISFEVDSDPRAAYFRQAENGMYIRMALLATVLGKY

Target ORF information:

RefSeq Version	XM_018262173.1
Organism	Xenopus laevis(African clawed frog)
Definition	Xenopus laevis CAD protein-like (LOC108716059), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_018262173.1

ORF Insert Sequence:

ATGGCTGCCG CTGCGTTTCT GCTGCTCGAG GATGGGACTC GCCTCCCCGG GAGGATGTTC 
GGGGCTCAGC GCTTTGTCTC CGGGGAAGTG GTGTTCCAGA CGGGCATGGT GGGCTACCCC 
GAGTCCCTGA CCGACCCCTC ATACAAGAGT CAGATCCTGG TGCTGACGTA TCCGCTCATA 
GGGAACTATG GGATCCCCCG CGATCAACTC GACCAGTTTG GGCTCAGCCG GTGGTTTGAG 
TCCAGTAAGA TTCATGTCTC GGCGCTGATT ATTGGGGAGC AATCGCAGAA CCCCAGTCAC 
TGGAGCTGTG ACATCTCTTT GAACCAGTGG CTGAAGCAGC AGGGAATCCC CGGACTGGAA 
GGTGTGGACA CCCGGGCGCT GACCAAGAAG ATCCGAGAGA AGGGCACCCT GCTGGGCAAG 
CTCATAGCCC CCGGCACCGA CGAGACACAA GTCCCTTATG AAGATCCCGG GAAGAGGAAC 
CTGGTGCAGG AGGTGTCACT GAAGGAGCCC CGAGTGTTCA ATTCTGGGGG TGCAGTGAGG 
GTCGCTGCCC TGGACTGTGG GCTGAAGTTC AACCAGATCC GCTGCCTGTG CCAGAGGGGG 
GCCGAGGTGA CGGTGCTGCC ATGGGATTAT CCAATCAGCG CTGCAGAGTT TGACGGACTG 
TTCCTCAGTA ACGGCCCCGG GGACCCCACT TTCTGCCAGA AATCCGTGCA GAACATCAGG 
GGGTTCATCT CACAGGAGAA GCCAAAACCC GTATTTGGGA TCTGTCTCGG CCACCAGATC 
CTCTCCCTGG TTATTGGAGC CAAGACCTAC AAGATGAAGT ACGGGAATCG TGGGCACAAC 
CAGCCGTGTA TACACGAGGG CTCTCAGCGC TGCTATATAA CCTCCCAGAA CCACGGATAC 
GCTGTGGACC CAAACTCCTT ACCTGAAGGC TGGTCTCCTC TGTTCACTAA TGCCAACGAC 
CAATCAAACG AGGGGATTAT ACATGACTCC AAACCCTTCT TCAGTGTGCA GTTCCACCCG 
GAGCACAGTG CTGGGCCCAT GGACCTGGTG TGTCTATTTG ATGTATTTCT GGAGACTGTG 
AGAGACATAA AGGCCGGGAA TTCGGGAGAG AAGTCAGTAA AACAGCGACT GACTGAGCTG 
CTCACGTACA AAGACTCCCC CGGTGTCACA GACTTGGAGG CCTCTCGTCC GCAAAAAGTG 
CTGATCCTGG GCTCAGGGGG GCTCTCGATA GGCCAGGCCG GGGAGTTCGA CTACTCTGGC 
TCTCAGGCCA TTAAAGCACT GAAGGAAGAG AACATCCAGA CAGTCCTGAT TAACCCCAAC 
ATTGCCACAG TACAGACATC AAAAGGCCTG GCCGACAAGG TTTATTTCCT GCCAATCACC 
CCTGAATATG TCACTCAGGT GATAAAGAAT GAGCGGCCAG ATGGAATACT CCTGACGTTT 
GGGGGTCAGA CTGCATTGAA TTGTGGGGTG GAGCTGACCA AGCAGGGGGT CCTAGAAAAG 
TACCATGTGC GTGTTCTGGG GACCCCTGTG AAATCCATTG AAATGACAGA AGATCGCAAA 
GTGTTTGTGG AGAAGATGGA GGAGATTGGA GAACACGTGG CCCCCAGTGA GGCGGCTTTC 
TCCTTGGAGC AGGCCCAGGC TGCTGCAGAA CGTCTCGGGT ATCCAGTTTT GGTCCGGTCA 
GCTTATGCAC TGGGTGGGCT AGGATCTGGA TTTGCCAATA ACAAGGAAGA GCTGGCTGCC 
CTGGTCTGCC AGGCCTTCGC ACACACAACG CAGGTCCTGG TGGATAAATC CCTGAAGGGC 
TGGAAGGAGA TTGAGTATGA AGTCGTGCGG GACGCCTATG ATAACTGTGT AACGGTTTGC 
AACATGGAGA ACGTGGATCC CCTTGGCATT CACACTGGAG AATCCATCGT GGTAGCTCCC 
AGCCAGACGC TGAACGATCG AGAGTACAAC ATGCTGCGCA ACACGGCCAT TAAGGTTATC 
CAGCACCTCG GCATTGTGGG CGAGTGCAAC ATACAGTACG CCTTAAATCC AGAATCTGAG 
CAGTATTATA TTATTGAGGT CAACGCAAGA CTGTCTCGTA GCTCAGCCCT GGCGAGTAAA 
GCAACCGGTT ACCCCCTGGC GTATGTGGCT GCCAAGCTGG CACTGGGAAT TCCACTGCCA 
GTTCTCAGGA ACTCTGTAAC CAACTCCACC ACGGCTAACT TTGAGCCCAG CCTGGATTAC 
TGTGTGGTGA AGGTTCCACG CTGGGACCTG AGCAAGTTCT CGCGAGTCAG TACCAAGATT 
GGAAGCTCCA TGAAGAGTGT GGGCGAGGTC ATGTCTGTTG GTAGGAGCTT TGAAGAAGCC 
TTCCAGAAAG CTCTGCGCAT GGTCGATGAG AACTGTGTGG GCTTCGATCA CTGCATCAGA 
TCTGCATCTG ATGAGGAACT GGAGACACCA ACAGATAAGC GAATCTTCGT GCTGGCCGCA 
GCCCTGAGAG TTGGTTACAC CATCGAGCGC CTCTATAAGT TGACCAGGAT TGACAAGTGG 
TTCCTCCATA AGATGAAGAA CATTACTGAT CACTTGGCCA CACTGGAGTC TTATCACCAG 
GATGAGACCT TGATGCCCCG TGAGGTCCTT CTCCGAGCCA AACAGCTGGG CTTCTCCGAT 
AAGCAGATTG CACTGGCCGT GCAAAGCACG GAGCTGGCAG TCCGGAAGAT GAGGCAGGAT 
CTGCAGATTT TGCCTGTAGT GAAGCAGATC GACACGGTAG CAGCGGAGTG GCCGGCTCAG 
ACCAATTACC TTTATCTGAC CTACAATGGC ATTGAGCATG ATTTGACCTT TCATGAGCCG 
CATGTTATGG TCATTGGCTC TGGTGTTTAC CGAATAGGTA GCAGCGTGGA GTTTGATTGG 
TGTGCAGTGG GCTGCATCCA GGAGCTGCGC AAGATGGGAT TTAAGACCAT CATGGTGAAT 
TACAATCCCG AGACTGTCAG CACAGACTAC GACATGTGTG ACCGCCTGTA TTTTGATGAG 
ATCTCGTTTG AGGTGGTGAT GGATATCTAT GAGCTTGAGA ACCCAGAGGG AATCATACTG 
TCCATGGGCG GCCAGCTACC CAATAACATG GCCATGTCCC TACATCGCCA GCAGTGCCGG 
GTACTGGGCA CCTCTCCTGA GGACATTGAC TCCGCTGAGA ACAGATTTAA GTTTTCTCGC 
CTTTTGGACA CAATTGGTAT TAGTCAGCCG CAGTGGAAAG AGCTGTCTGA CACGGAGTCA 
GCCGTTCAGT TCTGCAACAC TGTCGGCTTC CCCTGTGTTG TGCGTCCATC CTATGTGCTG 
AGCGGAGCCG CTATGAATGT GGCTTATTCA GATGAAGACA TGGAGAAGGT GCTTAGCAAT 
GCCGTGGCTG TATCCAAGGA GCATCCTGTG GTCATCTCTA AATTCATCCA GGAGGCCAAG 
GAAATTGATG TGGATGCCGT GGCCTGTGAT GGAGTTGTGG TTGCCGTGGC TATTTCGGAA 
CATGTGGAGA ATGCAGGGGT ACACTCTGGG GATGCAACAC TGGTGACCCC GGCTCAGGAT 
ATCACCCCCA AAACCTTGGA AAGGATTAAA GCCATTGTCC ACGCTGTGGG GCTGGAGCTC 
CAAGTCACCG GGCCCTTCAA CCTGCAACTC ATTGCTAAGG ATGACCAGCT GAAAGTGATT 
GAGTGCAACG TTCGTGTGTC TCGCTCCTTT CCCTTTGTGT CTAAGACTCT GGGGGTTGAT 
CTAGTAGCTC TGGCAACGCA AGTCATTATG GGAGAAGAGG TTGAACCTGT TGGTCTGATG 
ACTGGTACTG GCATTGTAGG GGTTAAGGTT CCACAGTTCT CGTTCTCACG CTTGGCTGGA 
GCAGACGTGG TCCTGGGGGT GGAGATGACC AGCACAGGGG AGGTGGCCTG CTTTGGAGAG 
AACCGATATG AGGCTTATCT AAAAGCCATG CTAAGTACTG GGTTCAAAAT CCCACAGAAG 
AACATCCTGC TGACTATTGG GAGCTATAAG AACAAGAGCG AGCTCCTGCC CACTGTGCGA 
GCCATGGAAA GCCTTGGGTA CAATCTGTAT GCCAGCCTGG GAACTGCTGA CTTCTACACC 
GAGCATGGCA TCAAGGTAAA GGCAATGGAC TGGCCCTTTG AGGAGAGCGA GAATGATGGG 
CCCCTACGGG AGAAGCAGGG GAGCATCATG GACCATTTGT CAGAGAATCA CTTTGATTTG 
GTCATTAACC TGTCCATGCG GAATTCTGGG GGTCGCCGAC TTTCCTCCTT TGTTACCCGT 
GGGTATCGTG CCCGCCGATT GGCTGTAGAC TATTCCGTGC CCCTCATCAT CGATATTAAG 
TGTACCAAAC TCTTTGTGGA GGCTCTGCGG CAGATTGGAC GGTCGCCCCC TGTTAAGACT 
CACGTTGATT GTATGACCTC ACAGAAGTTT ATCAGGCTGC CGGGCCTCAT TGATATCCAT 
GTGCACCTAC GGGAACCTGG AGCCACTCAT AAAGAGGACT TTTCTTCTGG CACAGCGGCT 
GCACTGGCTG GTGGAGTCAC CCTTGTATGT GCCATGCCGA ACACCAATCC AGCCATCACT 
GATGCCAGTT CCTTCTCTCT GGCACAGAAG CTGGCGGAGG CTGGTGCCCG CTGTGACTTT 
GCCCTTTTCC TTGGTGCTTC CTCAGAGAAT GCCGCTTCTC TTTCTCCAAT TGCCAGCTCA 
GCTGCTGGCC TTAAGATGTA TCTGAATGAC ACCTACTCTC AGCTTAAGAT GGACAATGTT 
TCTGTGTGGA TGGAGCACTT TGAGAAGTGG CCGGCTCACC TGCCCATTGT GGCACATGCA 
GAGAAACAGA CCGTGGCTGC GATTCTCATG GTGGCCCAAC TGTACCAGCG TCCTGTTCAC 
ATTTGCCACG TAGCAAAGAA AGAGGAGATC CTAATTATTA AAGCCGCCAA GCAGAAGGGA 
ATCAAAGTAA CATGTGAGGT GGCCCCACAT CACCTTTTCC TGTGCCTCAA TGACTTGGAG 
AAAATAGGAG AGGCACGGGG GCAAGTACGC CCCATGCTGG GGACTCGAGA GGACCTAGAA 
TCTCTGTGGG AAAACTTGGA TATTATAGAC TGTTTTGCCA CTGACCACGC CCCTCACTCT 
GTGGAGGAAA AGGATGGCCC AAACCCACCC CCTGGGTATC CAGGCTTGGA GACCATGTTG 
CCTCTGCTCC TCACAGCAGT CAGTGAGGGA CGTCTGACCA TTGATGACAT CATTAAGCGT 
CTGTATGACA ACCCCCGCAA GATCTTCTCC TTGCCTGTGC AGGAGAACAC TTATGTAGAG 
GTGGACTTGG AGCAAGAGTG GGTAATCCCA TCCCACATGC AGTTTACCAG GTCCAGGTGG 
ACCCCCTTTG CTGGAATGAA GGTGAAAGGA GCCGTGCGTC GAGTTGTGCT GCGTGGGGAG 
GTGGCCTACA TTGATGGCCA GGTGTTGGTA CCTGCAGGAT ATGGACAGGA TGTCAGGAAA 
TGGACCCCAC CCGTTGTTGC CCAATCTCAC CCCATGGTCA ATAAAGAGAT ACCCAAGACC 
CCAGAGCGGC ACCGCCATGT TACAGTACAA AGTGAGACCA TTCGCAGTCG AGCACCAAGC 
CCCCGCAGAC CTGTTCAGTC GGCAGATGGG CGCTTCTACT TGCCTCCTCG GGTGCATCGT 
GCTTCAGATC CTGGGTTACC TGCAGAGGAG CAGAAAGAGA AGGCACCCAA GAGATCCGTG 
GAGCCAGAGC TGGTGCCCAT CCAGGATGGC TATTCCCACC CTCCTCCTAT TCCTAGAATT 
ATGTCCCCCC AGAGTACGTC CATGCAAAGT GTCCCTCATG TGCAGACCTC ACCCCTGTTG 
CACCCACTGA TTGGCCAGCA CATCCTGACG GTTAAGCAGT TCAGCAAAGA CCAGATGTCC 
CACCTGTTTA ACGTGGCACA CACGCTGCGC ATATTGGTTC AGAAGGAGAG AAACCTAGAT 
ATGCTGAAGG GCAAGGTGAT GGCCTCCATG TTTTTTGAGG CCAGCACACG TACAAGCAGC 
TCCTTTGCTG CAGCGATGCA ACGTCTTGGG GGCTCTGTGC TCAGTTTCTG TGAGACAACC 
TCCTCTTCTC AAAAAGGAGA ATCACTCATT GACTCTGTTC AGACAATGAG CTGCTATTCC 
GATGTCCTTG TGGTGAGACA CCCAGAGCCT GGAGCTGTGG AGCTTGCTGC CAAGCACTGT 
CGGAAGCCTG TGATAAATGC TGGGGATGGA GTGGGCGAGC ACCCCACACA GGCACTACTG 
GACATTTTCA CAATCCGTGA AGAGCTGGGC ACAGTCAATG GAATGATAAT AACAATGGTC 
GGCGATTTGA AACATGGCAG GACAGTTCAC TCCCTCGCCT GCCTTCTAAC GCAGTACCGC 
GTGACTTTGA GATACGTGAC TCCCCGCAGC CTTCGTATGC CCTCCAACAT CATACACTTT 
GTAGCCTCCA AAGGCATTAA GCAGGAAGAG TTTGAGAGCA TTGAAGAAGC TCTGCCGGAT 
ACAGATGTGC TGTATATGAC CCGAATACAG AAGGAGAGAT TTAATTCTTC CGACGAGTAC 
AATGCTTGTT TTGGCCAATT TATCCTGACC CCACACATCA TGACCAGAGC CAAGAAGAGG 
ATGGTGGTAA TGCATCCGAT GCCTCGTGTG AACGAGATCA GTTTTGAAGT GGATTCCGAC 
CCCCGGGCAG CTTACTTCCG CCAGGCTGAG AACGGCATGT ATATCCGCAT GGCTCTCTTA 
GCAACAGTTT TGGGAAAATA CTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC108716059 ( XM_018262174.1 ) cDNA ORF clone, Xenopus laevis(African clawed frog) -> XP_018117663.1 Xenopus laevis CAD protein-like (LOC108716059), transcript variant X2, mRNA.

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CloneID	OAf31606
Clone ID Related Accession (Same CDS sequence)	XM_018262174.1
Accession Version	XM_018262174.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6699bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1474214400000
Organism	Xenopus laevis(African clawed frog)
Product	CAD protein-like isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030732.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus laevis Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTGCCG CTGCGTTTCT GCTGCTCGAG GATGGGACTC GCCTCCCCGG GAGGATGTTC 
GGGGCTCAGC GCTTTGTCTC CGGGGAAGTG GTGTTCCAGA CGGGCATGGT GGGCTACCCC 
GAGTCCCTGA CCGACCCCTC ATACAAGAGT CAGATCCTGG TGCTGACGTA TCCGCTCATA 
GGGAACTATG GGATCCCCCG CGATCAACTC GACCAGTTTG GGCTCAGCCG GTGGTTTGAG 
TCCAGTAAGA TTCATGTCTC GGCGCTGATT ATTGGGGAGC AATCGCAGAA CCCCAGTCAC 
TGGAGCTGTG ACATCTCTTT GAACCAGTGG CTGAAGCAGC AGGGAATCCC CGGACTGGAA 
GGTGTGGACA CCCGGGCGCT GACCAAGAAG ATCCGAGAGA AGGGCACCCT GCTGGGCAAG 
CTCATAGCCC CCGGCACCGA CGAGACACAA GTCCCTTATG AAGATCCCGG GAAGAGGAAC 
CTGGTGCAGG AGGTGTCACT GAAGGAGCCC CGAGTGTTCA ATTCTGGGGG TGCAGTGAGG 
GTCGCTGCCC TGGACTGTGG GCTGAAGTTC AACCAGATCC GCTGCCTGTG CCAGAGGGGG 
GCCGAGGTGA CGGTGCTGCC ATGGGATTAT CCAATCAGCG CTGCAGAGTT TGACGGACTG 
TTCCTCAGTA ACGGCCCCGG GGACCCCACT TTCTGCCAGA AATCCGTGCA GAACATCAGG 
GGGTTCATCT CACAGGAGAA GCCAAAACCC GTATTTGGGA TCTGTCTCGG CCACCAGATC 
CTCTCCCTGG TTATTGGAGC CAAGACCTAC AAGATGAAGT ACGGGAATCG TGGGCACAAC 
CAGCCGTGTA TACACGAGGG CTCTCAGCGC TGCTATATAA CCTCCCAGAA CCACGGATAC 
GCTGTGGACC CAAACTCCTT ACCTGAAGGC TGGTCTCCTC TGTTCACTAA TGCCAACGAC 
CAATCAAACG AGGGGATTAT ACATGACTCC AAACCCTTCT TCAGTGTGCA GTTCCACCCG 
GAGCACAGTG CTGGGCCCAT GGACCTGGTG TGTCTATTTG ATGTATTTCT GGAGACTGTG 
AGAGACATAA AGGCCGGGAA TTCGGGAGAG AAGTCAGTAA AACAGCGACT GACTGAGCTG 
CTCACGTACA AAGACTCCCC CGGTGTCACA GACTTGGAGG CCTCTCGTCC GCAAAAAGTG 
CTGATCCTGG GCTCAGGGGG GCTCTCGATA GGCCAGGCCG GGGAGTTCGA CTACTCTGGC 
TCTCAGGCCA TTAAAGCACT GAAGGAAGAG AACATCCAGA CAGTCCTGAT TAACCCCAAC 
ATTGCCACAG TACAGACATC AAAAGGCCTG GCCGACAAGG TTTATTTCCT GCCAATCACC 
CCTGAATATG TCACTCAGGT GATAAAGAAT GAGCGGCCAG ATGGAATACT CCTGACGTTT 
GGGGGTCAGA CTGCATTGAA TTGTGGGGTG GAGCTGACCA AGCAGGGGGT CCTAGAAAAG 
TACCATGTGC GTGTTCTGGG GACCCCTGTG AAATCCATTG AAATGACAGA AGATCGCAAA 
GTGTTTGTGG AGAAGATGGA GGAGATTGGA GAACACGTGG CCCCCAGTGA GGCGGCTTTC 
TCCTTGGAGC AGGCCCAGGC TGCTGCAGAA CGTCTCGGGT ATCCAGTTTT GGTCCGGTCA 
GCTTATGCAC TGGGTGGGCT AGGATCTGGA TTTGCCAATA ACAAGGAAGA GCTGGCTGCC 
CTGGTCTGCC AGGCCTTCGC ACACACAACG CAGGTCCTGG TGGATAAATC CCTGAAGGGC 
TGGAAGGAGA TTGAGTATGA AGTCGTGCGG GACGCCTATG ATAACTGTGT AACGGTTTGC 
AACATGGAGA ACGTGGATCC CCTTGGCATT CACACTGGAG AATCCATCGT GGTAGCTCCC 
AGCCAGACGC TGAACGATCG AGAGTACAAC ATGCTGCGCA ACACGGCCAT TAAGGTTATC 
CAGCACCTCG GCATTGTGGG CGAGTGCAAC ATACAGTACG CCTTAAATCC AGAATCTGAG 
CAGTATTATA TTATTGAGGT CAACGCAAGA CTGTCTCGTA GCTCAGCCCT GGCGAGTAAA 
GCAACCGGTT ACCCCCTGGC GTATGTGGCT GCCAAGCTGG CACTGGGAAT TCCACTGCCA 
GTTCTCAGGA ACTCTGTAAC CAACTCCACC ACGGCTAACT TTGAGCCCAG CCTGGATTAC 
TGTGTGGTGA AGGTTCCACG CTGGGACCTG AGCAAGTTCT CGCGAGTCAG TACCAAGATT 
GGAAGCTCCA TGAAGAGTGT GGGCGAGGTC ATGTCTGTTG GTAGGAGCTT TGAAGAAGCC 
TTCCAGAAAG CTCTGCGCAT GGTCGATGAG AACTGTGTGG GCTTCGATCA CTGCATCAGA 
TCTGCATCTG ATGAGGAACT GGAGACACCA ACAGATAAGC GAATCTTCGT GCTGGCCGCA 
GCCCTGAGAG TTGGTTACAC CATCGAGCGC CTCTATAAGT TGACCAGGAT TGACAAGTGG 
TTCCTCCATA AGATGAAGAA CATTACTGAT CACTTGGCCA CACTGGAGTC TTATCACCAG 
GATGAGACCT TGATGCCCCG TGAGGTCCTT CTCCGAGCCA AACAGCTGGG CTTCTCCGAT 
AAGCAGATTG CACTGGCCGT GCAAAGCACG GAGCTGGCAG TCCGGAAGAT GAGGCAGGAT 
CTGCAGATTT TGCCTGTAGT GAAGCAGATC GACACGGTAG CAGCGGAGTG GCCGGCTCAG 
ACCAATTACC TTTATCTGAC CTACAATGGC ATTGAGCATG ATTTGACCTT TCATGAGCCG 
CATGTTATGG TCATTGGCTC TGGTGTTTAC CGAATAGGTA GCAGCGTGGA GTTTGATTGG 
TGTGCAGTGG GCTGCATCCA GGAGCTGCGC AAGATGGGAT TTAAGACCAT CATGGTGAAT 
TACAATCCCG AGACTGTCAG CACAGACTAC GACATGTGTG ACCGCCTGTA TTTTGATGAG 
ATCTCGTTTG AGGTGGTGAT GGATATCTAT GAGCTTGAGA ACCCAGAGGG AATCATACTG 
TCCATGGGCG GCCAGCTACC CAATAACATG GCCATGTCCC TACATCGCCA GCAGTGCCGG 
GTACTGGGCA CCTCTCCTGA GGACATTGAC TCCGCTGAGA ACAGATTTAA GTTTTCTCGC 
CTTTTGGACA CAATTGGTAT TAGTCAGCCG CAGTGGAAAG AGCTGTCTGA CACGGAGTCA 
GCCGTTCAGT TCTGCAACAC TGTCGGCTTC CCCTGTGTTG TGCGTCCATC CTATGTGCTG 
AGCGGAGCCG CTATGAATGT GGCTTATTCA GATGAAGACA TGGAGAAGGT GCTTAGCAAT 
GCCGTGGCTG TATCCAAGGA GCATCCTGTG GTCATCTCTA AATTCATCCA GGAGGCCAAG 
GAAATTGATG TGGATGCCGT GGCCTGTGAT GGAGTTGTGG TTGCCGTGGC TATTTCGGAA 
CATGTGGAGA ATGCAGGGGT ACACTCTGGG GATGCAACAC TGGTGACCCC GGCTCAGGAT 
ATCACCCCCA AAACCTTGGA AAGGATTAAA GCCATTGTCC ACGCTGTGGG GCTGGAGCTC 
CAAGTCACCG GGCCCTTCAA CCTGCAACTC ATTGCTAAGG ATGACCAGCT GAAAGTGATT 
GAGTGCAACG TTCGTGTGTC TCGCTCCTTT CCCTTTGTGT CTAAGACTCT GGGGGTTGAT 
CTAGTAGCTC TGGCAACGCA AGTCATTATG GGAGAAGAGG TTGAACCTGT TGGTCTGATG 
ACTGGTACTG GCATTGTAGG GGTTAAGGTT CCACAGTTCT CGTTCTCACG CTTGGCTGGA 
GCAGACGTGG TCCTGGGGGT GGAGATGACC AGCACAGGGG AGGTGGCCTG CTTTGGAGAG 
AACCGATATG AGGCTTATCT AAAAGCCATG CTAAGTACTG GGTTCAAAAT CCCACAGAAG 
AACATCCTGC TGACTATTGG GAGCTATAAG AACAAGAGCG AGCTCCTGCC CACTGTGCGA 
GCCATGGAAA GCCTTGGGTA CAATCTGTAT GCCAGCCTGG GAACTGCTGA CTTCTACACC 
GAGCATGGCA TCAAGGTAAA GGCAATGGAC TGGCCCTTTG AGGAGAGCGA GAATGATGGG 
CCCCTACGGG AGAAGCAGGG GAGCATCATG GACCATTTGT CAGAGAATCA CTTTGATTTG 
GTCATTAACC TGTCCATGCG GAATTCTGGG GGTCGCCGAC TTTCCTCCTT TGTTACCCGT 
GGGTATCGTG CCCGCCGATT GGCTGTAGAC TATTCCGTGC CCCTCATCAT CGATATTAAG 
TGTACCAAAC TCTTTGTGGA GGCTCTGCGG CAGATTGGAC GGTCGCCCCC TGTTAAGACT 
CACGTTGATT GTATGACCTC ACAGAAGTTT ATCAGGCTGC CGGGCCTCAT TGATATCCAT 
GTGCACCTAC GGGAACCTGG AGCCACTCAT AAAGAGGACT TTTCTTCTGG CACAGCGGCT 
GCACTGGCTG GTGGAGTCAC CCTTGTATGT GCCATGCCGA ACACCAATCC AGCCATCACT 
GATGCCAGTT CCTTCTCTCT GGCACAGAAG CTGGCGGAGG CTGGTGCCCG CTGTGACTTT 
GCCCTTTTCC TTGGTGCTTC CTCAGAGAAT GCCGCTTCTC TTTCTCCAAT TGCCAGCTCA 
GCTGCTGGCC TTAAGATGTA TCTGAATGAC ACCTACTCTC AGCTTAAGAT GGACAATGTT 
TCTGTGTGGA TGGAGCACTT TGAGAAGTGG CCGGCTCACC TGCCCATTGT GGCACATGCA 
GAGAAACAGA CCGTGGCTGC GATTCTCATG GTGGCCCAAC TGTACCAGCG TCCTGTTCAC 
ATTTGCCACG TAGCAAAGAA AGAGGAGATC CTAATTATTA AAGCCGCCAA GCAGAAGGGA 
ATCAAAGTAA CATGTGAGGT GGCCCCACAT CACCTTTTCC TGTGCCTCAA TGACTTGGAG 
AAAATAGGAG AGGCACGGGG GCAAGTACGC CCCATGCTGG GGACTCGAGA GGACCTAGAA 
TCTCTGTGGG AAAACTTGGA TATTATAGAC TGTTTTGCCA CTGACCACGC CCCTCACTCT 
GTGGAGGAAA AGGATGGCCC AAACCCACCC CCTGGGTATC CAGGCTTGGA GACCATGTTG 
CCTCTGCTCC TCACAGCAGT CAGTGAGGGA CGTCTGACCA TTGATGACAT CATTAAGCGT 
CTGTATGACA ACCCCCGCAA GATCTTCTCC TTGCCTGTGC AGGAGAACAC TTATGTAGAG 
GTGGACTTGG AGCAAGAGTG GGTAATCCCA TCCCACATGC AGTTTACCAG GTCCAGGTGG 
ACCCCCTTTG CTGGAATGAA GGTGAAAGGA GCCGTGCGTC GAGTTGTGCT GCGTGGGGAG 
GTGGCCTACA TTGATGGCCA GGTGTTGGTA CCTGCAGGAT ATGGACAGGA TGTCAGGAAA 
TGGACCCCAC CCGTTGTTGC CCAATCTCAC CCCATGGTCA ATAAAGAGAT ACCCAAGACC 
CCAGAGCGGC ACCGCCATGT TACAGTACAA AGTGAGACCA TTCGCAGTCG AGCACCAAGC 
CCCCGCAGAC CTGTTCAGTC GGCAGATGGG CGCTTCTACT TGCCTCCTCG GGTGCATCGT 
GCTTCAGATC CTGGGTTACC TGAGCTGGTG CCCATCCAGG ATGGCTATTC CCACCCTCCT 
CCTATTCCTA GAATTATGTC CCCCCAGAGT ACGTCCATGC AAAGTGTCCC TCATGTGCAG 
ACCTCACCCC TGTTGCACCC ACTGATTGGC CAGCACATCC TGACGGTTAA GCAGTTCAGC 
AAAGACCAGA TGTCCCACCT GTTTAACGTG GCACACACGC TGCGCATATT GGTTCAGAAG 
GAGAGAAACC TAGATATGCT GAAGGGCAAG GTGATGGCCT CCATGTTTTT TGAGGCCAGC 
ACACGTACAA GCAGCTCCTT TGCTGCAGCG ATGCAACGTC TTGGGGGCTC TGTGCTCAGT 
TTCTGTGAGA CAACCTCCTC TTCTCAAAAA GGAGAATCAC TCATTGACTC TGTTCAGACA 
ATGAGCTGCT ATTCCGATGT CCTTGTGGTG AGACACCCAG AGCCTGGAGC TGTGGAGCTT 
GCTGCCAAGC ACTGTCGGAA GCCTGTGATA AATGCTGGGG ATGGAGTGGG CGAGCACCCC 
ACACAGGCAC TACTGGACAT TTTCACAATC CGTGAAGAGC TGGGCACAGT CAATGGAATG 
ATAATAACAA TGGTCGGCGA TTTGAAACAT GGCAGGACAG TTCACTCCCT CGCCTGCCTT 
CTAACGCAGT ACCGCGTGAC TTTGAGATAC GTGACTCCCC GCAGCCTTCG TATGCCCTCC 
AACATCATAC ACTTTGTAGC CTCCAAAGGC ATTAAGCAGG AAGAGTTTGA GAGCATTGAA 
GAAGCTCTGC CGGATACAGA TGTGCTGTAT ATGACCCGAA TACAGAAGGA GAGATTTAAT 
TCTTCCGACG AGTACAATGC TTGTTTTGGC CAATTTATCC TGACCCCACA CATCATGACC 
AGAGCCAAGA AGAGGATGGT GGTAATGCAT CCGATGCCTC GTGTGAACGA GATCAGTTTT 
GAAGTGGATT CCGACCCCCG GGCAGCTTAC TTCCGCCAGG CTGAGAACGG CATGTATATC 
CGCATGGCTC TCTTAGCAAC AGTTTTGGGA AAATACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_018117663.1
CDS	149..6847
Translation	MAAAAFLLLEDGTRLPGRMFGAQRFVSGEVVFQTGMVGYPESLTDPSYKSQILVLTYPLIGNYGIPRDQLDQFGLSRWFESSKIHVSALIIGEQSQNPSHWSCDISLNQWLKQQGIPGLEGVDTRALTKKIREKGTLLGKLIAPGTDETQVPYEDPGKRNLVQEVSLKEPRVFNSGGAVRVAALDCGLKFNQIRCLCQRGAEVTVLPWDYPISAAEFDGLFLSNGPGDPTFCQKSVQNIRGFISQEKPKPVFGICLGHQILSLVIGAKTYKMKYGNRGHNQPCIHEGSQRCYITSQNHGYAVDPNSLPEGWSPLFTNANDQSNEGIIHDSKPFFSVQFHPEHSAGPMDLVCLFDVFLETVRDIKAGNSGEKSVKQRLTELLTYKDSPGVTDLEASRPQKVLILGSGGLSIGQAGEFDYSGSQAIKALKEENIQTVLINPNIATVQTSKGLADKVYFLPITPEYVTQVIKNERPDGILLTFGGQTALNCGVELTKQGVLEKYHVRVLGTPVKSIEMTEDRKVFVEKMEEIGEHVAPSEAAFSLEQAQAAAERLGYPVLVRSAYALGGLGSGFANNKEELAALVCQAFAHTTQVLVDKSLKGWKEIEYEVVRDAYDNCVTVCNMENVDPLGIHTGESIVVAPSQTLNDREYNMLRNTAIKVIQHLGIVGECNIQYALNPESEQYYIIEVNARLSRSSALASKATGYPLAYVAAKLALGIPLPVLRNSVTNSTTANFEPSLDYCVVKVPRWDLSKFSRVSTKIGSSMKSVGEVMSVGRSFEEAFQKALRMVDENCVGFDHCIRSASDEELETPTDKRIFVLAAALRVGYTIERLYKLTRIDKWFLHKMKNITDHLATLESYHQDETLMPREVLLRAKQLGFSDKQIALAVQSTELAVRKMRQDLQILPVVKQIDTVAAEWPAQTNYLYLTYNGIEHDLTFHEPHVMVIGSGVYRIGSSVEFDWCAVGCIQELRKMGFKTIMVNYNPETVSTDYDMCDRLYFDEISFEVVMDIYELENPEGIILSMGGQLPNNMAMSLHRQQCRVLGTSPEDIDSAENRFKFSRLLDTIGISQPQWKELSDTESAVQFCNTVGFPCVVRPSYVLSGAAMNVAYSDEDMEKVLSNAVAVSKEHPVVISKFIQEAKEIDVDAVACDGVVVAVAISEHVENAGVHSGDATLVTPAQDITPKTLERIKAIVHAVGLELQVTGPFNLQLIAKDDQLKVIECNVRVSRSFPFVSKTLGVDLVALATQVIMGEEVEPVGLMTGTGIVGVKVPQFSFSRLAGADVVLGVEMTSTGEVACFGENRYEAYLKAMLSTGFKIPQKNILLTIGSYKNKSELLPTVRAMESLGYNLYASLGTADFYTEHGIKVKAMDWPFEESENDGPLREKQGSIMDHLSENHFDLVINLSMRNSGGRRLSSFVTRGYRARRLAVDYSVPLIIDIKCTKLFVEALRQIGRSPPVKTHVDCMTSQKFIRLPGLIDIHVHLREPGATHKEDFSSGTAAALAGGVTLVCAMPNTNPAITDASSFSLAQKLAEAGARCDFALFLGASSENAASLSPIASSAAGLKMYLNDTYSQLKMDNVSVWMEHFEKWPAHLPIVAHAEKQTVAAILMVAQLYQRPVHICHVAKKEEILIIKAAKQKGIKVTCEVAPHHLFLCLNDLEKIGEARGQVRPMLGTREDLESLWENLDIIDCFATDHAPHSVEEKDGPNPPPGYPGLETMLPLLLTAVSEGRLTIDDIIKRLYDNPRKIFSLPVQENTYVEVDLEQEWVIPSHMQFTRSRWTPFAGMKVKGAVRRVVLRGEVAYIDGQVLVPAGYGQDVRKWTPPVVAQSHPMVNKEIPKTPERHRHVTVQSETIRSRAPSPRRPVQSADGRFYLPPRVHRASDPGLPELVPIQDGYSHPPPIPRIMSPQSTSMQSVPHVQTSPLLHPLIGQHILTVKQFSKDQMSHLFNVAHTLRILVQKERNLDMLKGKVMASMFFEASTRTSSSFAAAMQRLGGSVLSFCETTSSSQKGESLIDSVQTMSCYSDVLVVRHPEPGAVELAAKHCRKPVINAGDGVGEHPTQALLDIFTIREELGTVNGMIITMVGDLKHGRTVHSLACLLTQYRVTLRYVTPRSLRMPSNIIHFVASKGIKQEEFESIEEALPDTDVLYMTRIQKERFNSSDEYNACFGQFILTPHIMTRAKKRMVVMHPMPRVNEISFEVDSDPRAAYFRQAENGMYIRMALLATVLGKY

Target ORF information:

RefSeq Version	XM_018262174.1
Organism	Xenopus laevis(African clawed frog)
Definition	Xenopus laevis CAD protein-like (LOC108716059), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_018262174.1

ORF Insert Sequence:

ATGGCTGCCG CTGCGTTTCT GCTGCTCGAG GATGGGACTC GCCTCCCCGG GAGGATGTTC 
GGGGCTCAGC GCTTTGTCTC CGGGGAAGTG GTGTTCCAGA CGGGCATGGT GGGCTACCCC 
GAGTCCCTGA CCGACCCCTC ATACAAGAGT CAGATCCTGG TGCTGACGTA TCCGCTCATA 
GGGAACTATG GGATCCCCCG CGATCAACTC GACCAGTTTG GGCTCAGCCG GTGGTTTGAG 
TCCAGTAAGA TTCATGTCTC GGCGCTGATT ATTGGGGAGC AATCGCAGAA CCCCAGTCAC 
TGGAGCTGTG ACATCTCTTT GAACCAGTGG CTGAAGCAGC AGGGAATCCC CGGACTGGAA 
GGTGTGGACA CCCGGGCGCT GACCAAGAAG ATCCGAGAGA AGGGCACCCT GCTGGGCAAG 
CTCATAGCCC CCGGCACCGA CGAGACACAA GTCCCTTATG AAGATCCCGG GAAGAGGAAC 
CTGGTGCAGG AGGTGTCACT GAAGGAGCCC CGAGTGTTCA ATTCTGGGGG TGCAGTGAGG 
GTCGCTGCCC TGGACTGTGG GCTGAAGTTC AACCAGATCC GCTGCCTGTG CCAGAGGGGG 
GCCGAGGTGA CGGTGCTGCC ATGGGATTAT CCAATCAGCG CTGCAGAGTT TGACGGACTG 
TTCCTCAGTA ACGGCCCCGG GGACCCCACT TTCTGCCAGA AATCCGTGCA GAACATCAGG 
GGGTTCATCT CACAGGAGAA GCCAAAACCC GTATTTGGGA TCTGTCTCGG CCACCAGATC 
CTCTCCCTGG TTATTGGAGC CAAGACCTAC AAGATGAAGT ACGGGAATCG TGGGCACAAC 
CAGCCGTGTA TACACGAGGG CTCTCAGCGC TGCTATATAA CCTCCCAGAA CCACGGATAC 
GCTGTGGACC CAAACTCCTT ACCTGAAGGC TGGTCTCCTC TGTTCACTAA TGCCAACGAC 
CAATCAAACG AGGGGATTAT ACATGACTCC AAACCCTTCT TCAGTGTGCA GTTCCACCCG 
GAGCACAGTG CTGGGCCCAT GGACCTGGTG TGTCTATTTG ATGTATTTCT GGAGACTGTG 
AGAGACATAA AGGCCGGGAA TTCGGGAGAG AAGTCAGTAA AACAGCGACT GACTGAGCTG 
CTCACGTACA AAGACTCCCC CGGTGTCACA GACTTGGAGG CCTCTCGTCC GCAAAAAGTG 
CTGATCCTGG GCTCAGGGGG GCTCTCGATA GGCCAGGCCG GGGAGTTCGA CTACTCTGGC 
TCTCAGGCCA TTAAAGCACT GAAGGAAGAG AACATCCAGA CAGTCCTGAT TAACCCCAAC 
ATTGCCACAG TACAGACATC AAAAGGCCTG GCCGACAAGG TTTATTTCCT GCCAATCACC 
CCTGAATATG TCACTCAGGT GATAAAGAAT GAGCGGCCAG ATGGAATACT CCTGACGTTT 
GGGGGTCAGA CTGCATTGAA TTGTGGGGTG GAGCTGACCA AGCAGGGGGT CCTAGAAAAG 
TACCATGTGC GTGTTCTGGG GACCCCTGTG AAATCCATTG AAATGACAGA AGATCGCAAA 
GTGTTTGTGG AGAAGATGGA GGAGATTGGA GAACACGTGG CCCCCAGTGA GGCGGCTTTC 
TCCTTGGAGC AGGCCCAGGC TGCTGCAGAA CGTCTCGGGT ATCCAGTTTT GGTCCGGTCA 
GCTTATGCAC TGGGTGGGCT AGGATCTGGA TTTGCCAATA ACAAGGAAGA GCTGGCTGCC 
CTGGTCTGCC AGGCCTTCGC ACACACAACG CAGGTCCTGG TGGATAAATC CCTGAAGGGC 
TGGAAGGAGA TTGAGTATGA AGTCGTGCGG GACGCCTATG ATAACTGTGT AACGGTTTGC 
AACATGGAGA ACGTGGATCC CCTTGGCATT CACACTGGAG AATCCATCGT GGTAGCTCCC 
AGCCAGACGC TGAACGATCG AGAGTACAAC ATGCTGCGCA ACACGGCCAT TAAGGTTATC 
CAGCACCTCG GCATTGTGGG CGAGTGCAAC ATACAGTACG CCTTAAATCC AGAATCTGAG 
CAGTATTATA TTATTGAGGT CAACGCAAGA CTGTCTCGTA GCTCAGCCCT GGCGAGTAAA 
GCAACCGGTT ACCCCCTGGC GTATGTGGCT GCCAAGCTGG CACTGGGAAT TCCACTGCCA 
GTTCTCAGGA ACTCTGTAAC CAACTCCACC ACGGCTAACT TTGAGCCCAG CCTGGATTAC 
TGTGTGGTGA AGGTTCCACG CTGGGACCTG AGCAAGTTCT CGCGAGTCAG TACCAAGATT 
GGAAGCTCCA TGAAGAGTGT GGGCGAGGTC ATGTCTGTTG GTAGGAGCTT TGAAGAAGCC 
TTCCAGAAAG CTCTGCGCAT GGTCGATGAG AACTGTGTGG GCTTCGATCA CTGCATCAGA 
TCTGCATCTG ATGAGGAACT GGAGACACCA ACAGATAAGC GAATCTTCGT GCTGGCCGCA 
GCCCTGAGAG TTGGTTACAC CATCGAGCGC CTCTATAAGT TGACCAGGAT TGACAAGTGG 
TTCCTCCATA AGATGAAGAA CATTACTGAT CACTTGGCCA CACTGGAGTC TTATCACCAG 
GATGAGACCT TGATGCCCCG TGAGGTCCTT CTCCGAGCCA AACAGCTGGG CTTCTCCGAT 
AAGCAGATTG CACTGGCCGT GCAAAGCACG GAGCTGGCAG TCCGGAAGAT GAGGCAGGAT 
CTGCAGATTT TGCCTGTAGT GAAGCAGATC GACACGGTAG CAGCGGAGTG GCCGGCTCAG 
ACCAATTACC TTTATCTGAC CTACAATGGC ATTGAGCATG ATTTGACCTT TCATGAGCCG 
CATGTTATGG TCATTGGCTC TGGTGTTTAC CGAATAGGTA GCAGCGTGGA GTTTGATTGG 
TGTGCAGTGG GCTGCATCCA GGAGCTGCGC AAGATGGGAT TTAAGACCAT CATGGTGAAT 
TACAATCCCG AGACTGTCAG CACAGACTAC GACATGTGTG ACCGCCTGTA TTTTGATGAG 
ATCTCGTTTG AGGTGGTGAT GGATATCTAT GAGCTTGAGA ACCCAGAGGG AATCATACTG 
TCCATGGGCG GCCAGCTACC CAATAACATG GCCATGTCCC TACATCGCCA GCAGTGCCGG 
GTACTGGGCA CCTCTCCTGA GGACATTGAC TCCGCTGAGA ACAGATTTAA GTTTTCTCGC 
CTTTTGGACA CAATTGGTAT TAGTCAGCCG CAGTGGAAAG AGCTGTCTGA CACGGAGTCA 
GCCGTTCAGT TCTGCAACAC TGTCGGCTTC CCCTGTGTTG TGCGTCCATC CTATGTGCTG 
AGCGGAGCCG CTATGAATGT GGCTTATTCA GATGAAGACA TGGAGAAGGT GCTTAGCAAT 
GCCGTGGCTG TATCCAAGGA GCATCCTGTG GTCATCTCTA AATTCATCCA GGAGGCCAAG 
GAAATTGATG TGGATGCCGT GGCCTGTGAT GGAGTTGTGG TTGCCGTGGC TATTTCGGAA 
CATGTGGAGA ATGCAGGGGT ACACTCTGGG GATGCAACAC TGGTGACCCC GGCTCAGGAT 
ATCACCCCCA AAACCTTGGA AAGGATTAAA GCCATTGTCC ACGCTGTGGG GCTGGAGCTC 
CAAGTCACCG GGCCCTTCAA CCTGCAACTC ATTGCTAAGG ATGACCAGCT GAAAGTGATT 
GAGTGCAACG TTCGTGTGTC TCGCTCCTTT CCCTTTGTGT CTAAGACTCT GGGGGTTGAT 
CTAGTAGCTC TGGCAACGCA AGTCATTATG GGAGAAGAGG TTGAACCTGT TGGTCTGATG 
ACTGGTACTG GCATTGTAGG GGTTAAGGTT CCACAGTTCT CGTTCTCACG CTTGGCTGGA 
GCAGACGTGG TCCTGGGGGT GGAGATGACC AGCACAGGGG AGGTGGCCTG CTTTGGAGAG 
AACCGATATG AGGCTTATCT AAAAGCCATG CTAAGTACTG GGTTCAAAAT CCCACAGAAG 
AACATCCTGC TGACTATTGG GAGCTATAAG AACAAGAGCG AGCTCCTGCC CACTGTGCGA 
GCCATGGAAA GCCTTGGGTA CAATCTGTAT GCCAGCCTGG GAACTGCTGA CTTCTACACC 
GAGCATGGCA TCAAGGTAAA GGCAATGGAC TGGCCCTTTG AGGAGAGCGA GAATGATGGG 
CCCCTACGGG AGAAGCAGGG GAGCATCATG GACCATTTGT CAGAGAATCA CTTTGATTTG 
GTCATTAACC TGTCCATGCG GAATTCTGGG GGTCGCCGAC TTTCCTCCTT TGTTACCCGT 
GGGTATCGTG CCCGCCGATT GGCTGTAGAC TATTCCGTGC CCCTCATCAT CGATATTAAG 
TGTACCAAAC TCTTTGTGGA GGCTCTGCGG CAGATTGGAC GGTCGCCCCC TGTTAAGACT 
CACGTTGATT GTATGACCTC ACAGAAGTTT ATCAGGCTGC CGGGCCTCAT TGATATCCAT 
GTGCACCTAC GGGAACCTGG AGCCACTCAT AAAGAGGACT TTTCTTCTGG CACAGCGGCT 
GCACTGGCTG GTGGAGTCAC CCTTGTATGT GCCATGCCGA ACACCAATCC AGCCATCACT 
GATGCCAGTT CCTTCTCTCT GGCACAGAAG CTGGCGGAGG CTGGTGCCCG CTGTGACTTT 
GCCCTTTTCC TTGGTGCTTC CTCAGAGAAT GCCGCTTCTC TTTCTCCAAT TGCCAGCTCA 
GCTGCTGGCC TTAAGATGTA TCTGAATGAC ACCTACTCTC AGCTTAAGAT GGACAATGTT 
TCTGTGTGGA TGGAGCACTT TGAGAAGTGG CCGGCTCACC TGCCCATTGT GGCACATGCA 
GAGAAACAGA CCGTGGCTGC GATTCTCATG GTGGCCCAAC TGTACCAGCG TCCTGTTCAC 
ATTTGCCACG TAGCAAAGAA AGAGGAGATC CTAATTATTA AAGCCGCCAA GCAGAAGGGA 
ATCAAAGTAA CATGTGAGGT GGCCCCACAT CACCTTTTCC TGTGCCTCAA TGACTTGGAG 
AAAATAGGAG AGGCACGGGG GCAAGTACGC CCCATGCTGG GGACTCGAGA GGACCTAGAA 
TCTCTGTGGG AAAACTTGGA TATTATAGAC TGTTTTGCCA CTGACCACGC CCCTCACTCT 
GTGGAGGAAA AGGATGGCCC AAACCCACCC CCTGGGTATC CAGGCTTGGA GACCATGTTG 
CCTCTGCTCC TCACAGCAGT CAGTGAGGGA CGTCTGACCA TTGATGACAT CATTAAGCGT 
CTGTATGACA ACCCCCGCAA GATCTTCTCC TTGCCTGTGC AGGAGAACAC TTATGTAGAG 
GTGGACTTGG AGCAAGAGTG GGTAATCCCA TCCCACATGC AGTTTACCAG GTCCAGGTGG 
ACCCCCTTTG CTGGAATGAA GGTGAAAGGA GCCGTGCGTC GAGTTGTGCT GCGTGGGGAG 
GTGGCCTACA TTGATGGCCA GGTGTTGGTA CCTGCAGGAT ATGGACAGGA TGTCAGGAAA 
TGGACCCCAC CCGTTGTTGC CCAATCTCAC CCCATGGTCA ATAAAGAGAT ACCCAAGACC 
CCAGAGCGGC ACCGCCATGT TACAGTACAA AGTGAGACCA TTCGCAGTCG AGCACCAAGC 
CCCCGCAGAC CTGTTCAGTC GGCAGATGGG CGCTTCTACT TGCCTCCTCG GGTGCATCGT 
GCTTCAGATC CTGGGTTACC TGAGCTGGTG CCCATCCAGG ATGGCTATTC CCACCCTCCT 
CCTATTCCTA GAATTATGTC CCCCCAGAGT ACGTCCATGC AAAGTGTCCC TCATGTGCAG 
ACCTCACCCC TGTTGCACCC ACTGATTGGC CAGCACATCC TGACGGTTAA GCAGTTCAGC 
AAAGACCAGA TGTCCCACCT GTTTAACGTG GCACACACGC TGCGCATATT GGTTCAGAAG 
GAGAGAAACC TAGATATGCT GAAGGGCAAG GTGATGGCCT CCATGTTTTT TGAGGCCAGC 
ACACGTACAA GCAGCTCCTT TGCTGCAGCG ATGCAACGTC TTGGGGGCTC TGTGCTCAGT 
TTCTGTGAGA CAACCTCCTC TTCTCAAAAA GGAGAATCAC TCATTGACTC TGTTCAGACA 
ATGAGCTGCT ATTCCGATGT CCTTGTGGTG AGACACCCAG AGCCTGGAGC TGTGGAGCTT 
GCTGCCAAGC ACTGTCGGAA GCCTGTGATA AATGCTGGGG ATGGAGTGGG CGAGCACCCC 
ACACAGGCAC TACTGGACAT TTTCACAATC CGTGAAGAGC TGGGCACAGT CAATGGAATG 
ATAATAACAA TGGTCGGCGA TTTGAAACAT GGCAGGACAG TTCACTCCCT CGCCTGCCTT 
CTAACGCAGT ACCGCGTGAC TTTGAGATAC GTGACTCCCC GCAGCCTTCG TATGCCCTCC 
AACATCATAC ACTTTGTAGC CTCCAAAGGC ATTAAGCAGG AAGAGTTTGA GAGCATTGAA 
GAAGCTCTGC CGGATACAGA TGTGCTGTAT ATGACCCGAA TACAGAAGGA GAGATTTAAT 
TCTTCCGACG AGTACAATGC TTGTTTTGGC CAATTTATCC TGACCCCACA CATCATGACC 
AGAGCCAAGA AGAGGATGGT GGTAATGCAT CCGATGCCTC GTGTGAACGA GATCAGTTTT 
GAAGTGGATT CCGACCCCCG GGCAGCTTAC TTCCGCCAGG CTGAGAACGG CATGTATATC 
CGCATGGCTC TCTTAGCAAC AGTTTTGGGA AAATACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

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LOC108716059 cDNA ORF clone, Xenopus laevis(African clawed frog)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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