rlim.S cDNA ORF clone, Xenopus laevis(African clawed frog)

Gene
Clones
gRNAs

Summary

Gene Symbol	rlim.S
Entrez Gene ID	100137618
Full Name	ring finger protein, LIM domain interacting L homeolog
Synonyms	XRnf12,XRnf12C,rlim-a,rlim-b,rlim.S,rnf12,rnf12-B,rnf12b,xrnf12b
General protein information	Preferred Names ring finger protein, LIM domain interacting L homeolog Names E3 ubiquitin-protein ligase RNF12-B RING finger protein 12-B RING-type E3 ubiquitin transferase RNF12-B ring finger protein, LIM domain interacting S homeolog
Gene Type	protein-coding
Organism	Xenopus laevis(African clawed frog)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001114772.1	NP_001108244.1	E3 ubiquitin-protein ligase RNF12-B
XM_018228516.1	XP_018084005.1	E3 ubiquitin-protein ligase RNF12-B isoform X1
XM_018228517.1	XP_018084006.1	E3 ubiquitin-protein ligase RNF12-B isoform X2

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Selective degradation of excess Ldb1 by Rnf12/RLIM confers proper Ldb1 expression levels and Xlim-1/Ldb1 stoichiometry in Xenopus organizer functions.
Hiratani I, Yamamoto N, Mochizuki T, Ohmori SY, Taira M
Development (Cambridge, England)130(17)4161-75(2003 Sep)

Genetic and genomic tools for Xenopus research: The NIH Xenopus initiative.
Klein SL, Strausberg RL, Wagner L, Pontius J, Clifton SW, Richardson P
Developmental dynamics : an official publication of the American Association of Anatomists225(4)384-91(2002 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following rlim.S gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the rlim.S cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OAf03233	NM_001114772.1 Latest version!	Xenopus laevis ring finger protein, LIM domain interacting L homeolog (rlim.L), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
OAf40887	XM_018228516.1 Latest version!	Xenopus laevis ring finger protein, LIM domain interacting S homeolog (rlim.S), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
OAf40888	XM_018228517.1 Latest version!	Xenopus laevis ring finger protein, LIM domain interacting S homeolog (rlim.S), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

rlim.S ( NM_001114772.1 ) cDNA ORF clone, Xenopus laevis(African clawed frog) -> NP_001108244.1 Xenopus laevis ring finger protein, LIM domain interacting L homeolog (rlim.L), mRNA.

Price & Availability↑

CloneID	OAf03233
Clone ID Related Accession (Same CDS sequence)	NM_001114772.1
Accession Version	NM_001114772.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2274bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1577289600000
Organism	Xenopus laevis(African clawed frog)
Product	E3 ubiquitin-protein ligase RNF12-B
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AB114040.1. ##Evidence-Data-START## Transcript exon combination :: AB114040.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMD00012418, SAMD00012419 [ECO:0000348] ##Evidence-Data-END##

ATGGAGAGTG CGGATTCCAC CGGAAAAGGG AGCACTGAAC AGTCAGAGTC TCAGCGCCAA 
AGCCAGATGG ACAGGCTAGA TCGAGAGGAA GCTTTTTATC AGTTTGTAAA TAACTTAAAT 
GATGAGGACT ACAGACTAAT GAGAGATAAC AACTTATTGG GGACTCCAGG GGAAATTACT 
AAAGAGGAAT TGTTGCAAAG GCTTCAGCAA ATCAAAGAAG GGCCACCACA GCCAAGTACA 
GAAGAAACAA GAGGCGATTC TGTCTCCACA GGTGAGGACC CTGCAGAGGA TTCATCCAAT 
GGAGATTCCA TAATAGACTG GCTGAATTCA GTGCGACAGA CTGGGAACAC AACACGAAGT 
GGTCAGAGAG GCAATCAGTC CTGGAGGGCA GTGAGCAGGA CAAATCCAAA CAGTGGGGAT 
TTCAGGTTTA GTTTGGAAAT CAATGTGAAT CGTACTTCTG GCACCGCAAG CATGCCTTCG 
CTTGACCAAT CGGCTGAAAT GCCTGGGCCT GAGGATATGG AAGTGAGTAG TCAGGGTGAG 
GCTGAGAATG TACCTGAACC TGAAACTGTA CCTGAATCTA TACGTGAACC TGAATCTGTA 
GATGAACCTG TATCGGTAGC TGAACCTGTA TCGGTAGCTG AACCTGTATC GGTAGCTGAA 
CCTGAATCGG TAGCTGAACC TGAATCTGTA GCTGCATCTG TACCTGTACC TGAATCTGTA 
CCTGAACCTG AATCTGTACC TGAACCTGAA TCTGTACCTG AACCTGAATC TGTACCTGAA 
CCTGAATCTG TACCTGAACC TGAATCTGTA CCTGAACCTG AATCTGTACC TGAACCTGAA 
TCTGTACCTG AACCTGAATC TGTACCTGAA CCTGAATCTG TACCTGAACC TGAATCTGTA 
CCTGAACCTG AATCTGTACC TGAACCTGAA TCAATAGCTG AACCTGAATC TGTACCTGTA 
CCTGAATCTG TACCTGTTGC CACAAGGCCA GCACCAGTAG AGGTGACAGT AGAGGAAGCT 
CCAATACAGA GGGGGCAAAG GAGGGCCAGA AGCAGGAGTC CGGATCAGCG CAGGACGCGA 
GCTAGAACCG ATAGAAGCAG ATCCCCTCTA TATCAAACTG TTGATCCACC TATTCGAAGG 
GCTCAGCATA GTTCATCACA GACTGTTGAT GCTTCCAATA CAGAAGAAGC AGAGGGTAGC 
TCTAGGACTA GGCACCATGT TAGTTCTCAA GTGCACAGCT CTTCATCAAA TGAAACAGAG 
GGTAGTTCTA GAACTAGACA GCATATTACA GCCAGACAAC AAGCTCTTGG GACTGAAGGT 
CAGAGCCAAA GCACTGTTCA TCTGTCAAAC CCCGAGTCAA GAAGCTCTTC CCAAACTCCT 
CAGACAGACT CCCCCAGTAA TGCAGAGACT ACAGGTACCG GGCAGAGGCC TCCCACCATT 
GTGCTAGATC TGCAAGTAAG GCGCGTGCGC CCAGGTGATT ACAGGCAGAG AGACAGCATA 
GCTAACCGGA CACGTTCACG CTCTCAGACT CCCAACAACA CTGTCACATA TGAGAGCGAG 
AGAGGAGGAT TTCGTCGCAC TTTCTCGCGG TCTGAACGTG CCGGGGTTAG AACCTATGTA 
AGTACCATCC GAATTCCAAT CCGAAGAATC CTGAACACTG GGCTCAGTGA GACCACATCT 
GTAGCCATCC AGACAATGCT GAGGCAAATA ATGACTGGCT TTGGGGAACT CAGCTACTTC 
ATGTACAATG ATAACGACGC TGATCCTAAC AACCCCACAG CAGTATCTCC TCCTGCAGCA 
GTTCCTGGTG AAGTACAAAA CAACCCAAGC GCAGAGGTCA GAGCTCCTAT TGCTGAGCCT 
GCAGAACCTG TTGCTCCAGT GGAAAGTGAT GAAGGCAGCA ATGTTGCAAC ATCTGCAACC 
AGGAGGGAGG GTCGCAATAG CAGAGGTGGT GTCACTTTGG AAGAAAGCGG ATCTCTACCT 
TTTCTTAGTC TGGCCCAGTT TTTCCTGCTT AATGAGGATG ATGATGATCA GCCAAGAGGA 
CTCACCAAAG AGCAAATCGA CAACCTGTCC ACCCGAAATT ATGGTGAAAA TGATGCCCTG 
AAAACCTGTA GCGTGTGCAT AACAGAATAT ACAGAAGGGA ACAAGCTGCG CAAACTGCCT 
TGTTCCCACG AGTACCACAT CCACTGTATT GACCGCTGGT TGTCCGAAAA TTCTACCTGT 
CCCATCTGTC GCCGAGCAGT CCTGGTTGCA GGCAACAGAG AGAGCATTGT GTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001108244.1
CDS	167..2440
Translation	MESADSTGKGSTEQSESQRQSQMDRLDREEAFYQFVNNLNDEDYRLMRDNNLLGTPGEITKEELLQRLQQIKEGPPQPSTEETRGDSVSTGEDPAEDSSNGDSIIDWLNSVRQTGNTTRSGQRGNQSWRAVSRTNPNSGDFRFSLEINVNRTSGTASMPSLDQSAEMPGPEDMEVSSQGEAENVPEPETVPESIREPESVDEPVSVAEPVSVAEPVSVAEPESVAEPESVAASVPVPESVPEPESVPEPESVPEPESVPEPESVPEPESVPEPESVPEPESVPEPESVPEPESVPEPESVPEPESVPEPESIAEPESVPVPESVPVATRPAPVEVTVEEAPIQRGQRRARSRSPDQRRTRARTDRSRSPLYQTVDPPIRRAQHSSSQTVDASNTEEAEGSSRTRHHVSSQVHSSSSNETEGSSRTRQHITARQQALGTEGQSQSTVHLSNPESRSSSQTPQTDSPSNAETTGTGQRPPTIVLDLQVRRVRPGDYRQRDSIANRTRSRSQTPNNTVTYESERGGFRRTFSRSERAGVRTYVSTIRIPIRRILNTGLSETTSVAIQTMLRQIMTGFGELSYFMYNDNDADPNNPTAVSPPAAVPGEVQNNPSAEVRAPIAEPAEPVAPVESDEGSNVATSATRREGRNSRGGVTLEESGSLPFLSLAQFFLLNEDDDDQPRGLTKEQIDNLSTRNYGENDALKTCSVCITEYTEGNKLRKLPCSHEYHIHCIDRWLSENSTCPICRRAVLVAGNRESIV

Target ORF information:

RefSeq Version	NM_001114772.1
Organism	Xenopus laevis(African clawed frog)
Definition	Xenopus laevis ring finger protein, LIM domain interacting L homeolog (rlim.L), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001114772.1

ORF Insert Sequence:

ATGGAGAGTG CGGATTCCAC CGGAAAAGGG AGCACTGAAC AGTCAGAGTC TCAGCGCCAA 
AGCCAGATGG ACAGGCTAGA TCGAGAGGAA GCTTTTTATC AGTTTGTAAA TAACTTAAAT 
GATGAGGACT ACAGACTAAT GAGAGATAAC AACTTATTGG GGACTCCAGG GGAAATTACT 
AAAGAGGAAT TGTTGCAAAG GCTTCAGCAA ATCAAAGAAG GGCCACCACA GCCAAGTACA 
GAAGAAACAA GAGGCGATTC TGTCTCCACA GGTGAGGACC CTGCAGAGGA TTCATCCAAT 
GGAGATTCCA TAATAGACTG GCTGAATTCA GTGCGACAGA CTGGGAACAC AACACGAAGT 
GGTCAGAGAG GCAATCAGTC CTGGAGGGCA GTGAGCAGGA CAAATCCAAA CAGTGGGGAT 
TTCAGGTTTA GTTTGGAAAT CAATGTGAAT CGTACTTCTG GCACCGCAAG CATGCCTTCG 
CTTGACCAAT CGGCTGAAAT GCCTGGGCCT GAGGATATGG AAGTGAGTAG TCAGGGTGAG 
GCTGAGAATG TACCTGAACC TGAAACTGTA CCTGAATCTA TACGTGAACC TGAATCTGTA 
GATGAACCTG TATCGGTAGC TGAACCTGTA TCGGTAGCTG AACCTGTATC GGTAGCTGAA 
CCTGAATCGG TAGCTGAACC TGAATCTGTA GCTGCATCTG TACCTGTACC TGAATCTGTA 
CCTGAACCTG AATCTGTACC TGAACCTGAA TCTGTACCTG AACCTGAATC TGTACCTGAA 
CCTGAATCTG TACCTGAACC TGAATCTGTA CCTGAACCTG AATCTGTACC TGAACCTGAA 
TCTGTACCTG AACCTGAATC TGTACCTGAA CCTGAATCTG TACCTGAACC TGAATCTGTA 
CCTGAACCTG AATCTGTACC TGAACCTGAA TCAATAGCTG AACCTGAATC TGTACCTGTA 
CCTGAATCTG TACCTGTTGC CACAAGGCCA GCACCAGTAG AGGTGACAGT AGAGGAAGCT 
CCAATACAGA GGGGGCAAAG GAGGGCCAGA AGCAGGAGTC CGGATCAGCG CAGGACGCGA 
GCTAGAACCG ATAGAAGCAG ATCCCCTCTA TATCAAACTG TTGATCCACC TATTCGAAGG 
GCTCAGCATA GTTCATCACA GACTGTTGAT GCTTCCAATA CAGAAGAAGC AGAGGGTAGC 
TCTAGGACTA GGCACCATGT TAGTTCTCAA GTGCACAGCT CTTCATCAAA TGAAACAGAG 
GGTAGTTCTA GAACTAGACA GCATATTACA GCCAGACAAC AAGCTCTTGG GACTGAAGGT 
CAGAGCCAAA GCACTGTTCA TCTGTCAAAC CCCGAGTCAA GAAGCTCTTC CCAAACTCCT 
CAGACAGACT CCCCCAGTAA TGCAGAGACT ACAGGTACCG GGCAGAGGCC TCCCACCATT 
GTGCTAGATC TGCAAGTAAG GCGCGTGCGC CCAGGTGATT ACAGGCAGAG AGACAGCATA 
GCTAACCGGA CACGTTCACG CTCTCAGACT CCCAACAACA CTGTCACATA TGAGAGCGAG 
AGAGGAGGAT TTCGTCGCAC TTTCTCGCGG TCTGAACGTG CCGGGGTTAG AACCTATGTA 
AGTACCATCC GAATTCCAAT CCGAAGAATC CTGAACACTG GGCTCAGTGA GACCACATCT 
GTAGCCATCC AGACAATGCT GAGGCAAATA ATGACTGGCT TTGGGGAACT CAGCTACTTC 
ATGTACAATG ATAACGACGC TGATCCTAAC AACCCCACAG CAGTATCTCC TCCTGCAGCA 
GTTCCTGGTG AAGTACAAAA CAACCCAAGC GCAGAGGTCA GAGCTCCTAT TGCTGAGCCT 
GCAGAACCTG TTGCTCCAGT GGAAAGTGAT GAAGGCAGCA ATGTTGCAAC ATCTGCAACC 
AGGAGGGAGG GTCGCAATAG CAGAGGTGGT GTCACTTTGG AAGAAAGCGG ATCTCTACCT 
TTTCTTAGTC TGGCCCAGTT TTTCCTGCTT AATGAGGATG ATGATGATCA GCCAAGAGGA 
CTCACCAAAG AGCAAATCGA CAACCTGTCC ACCCGAAATT ATGGTGAAAA TGATGCCCTG 
AAAACCTGTA GCGTGTGCAT AACAGAATAT ACAGAAGGGA ACAAGCTGCG CAAACTGCCT 
TGTTCCCACG AGTACCACAT CCACTGTATT GACCGCTGGT TGTCCGAAAA TTCTACCTGT 
CCCATCTGTC GCCGAGCAGT CCTGGTTGCA GGCAACAGAG AGAGCATTGT GTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

rlim.S ( XM_018228516.1 ) cDNA ORF clone, Xenopus laevis(African clawed frog) -> XP_018084005.1 Xenopus laevis ring finger protein, LIM domain interacting S homeolog (rlim.S), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OAf40887
Clone ID Related Accession (Same CDS sequence)	XM_018228516.1
Accession Version	XM_018228516.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2325bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1474214400000
Organism	Xenopus laevis(African clawed frog)
Product	E3 ubiquitin-protein ligase RNF12-B isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030738.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus laevis Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGGAAGC TGACGGTAGC GAAGCGGGAT TCACGTTGGA CAACATCATC ATTGTATCCA 
TTCATCATCA TGGAGAGTGC GGATTCCACC GGAAAAGGGA GCACTGAACA GTCCGAGTCT 
CAGCGCCAAA GCCAGATGGA CAGGCTAGAT CGAGAGGAAG CTTTTTATCA GTTTGTAAAT 
AACTTAAATG ATGAGGACTA CAGACTAATG AGAGATAACA ACTTATTGGG GACTCCAGGG 
GAAATTACTA AAGAGGAATT GTTGCAAAGG CTTCAGCAAA TCAAAGAAGG GCCACCACAG 
CCAAGTACAG AAGAAACAAG AGGCGATTCT GTCTCCACAG GTGAGGACCC TGCAGAGGAT 
TCATCCAATG GAGATTCCAT AATAGACTGG CTGAATTCAG TGCGACAGAC TGGGAACACA 
ACACGAAGTG GTCAGAGAGG CAATCAGTCC TGGAGGGCAG TGAGCAGGAC AAATCCAAAC 
AGTGGGGATT TCAGGTTTAG TTTGGAAATC AATGTGAATC GTACTTCTGG CACCGCAAGC 
ATGCCTTCGC TTGACCAATC GGCTGAAATG CCTGGGCCTG AGGATATGGA AGTGAGTAGT 
CAGGGTGAGG CTGAGAATGT ACCTGAACCT GAAACTGTAC CTGAATCTAT ACGTGAACCT 
GAATCTGTAG ATGAACCTGT ATCGGTAGCT GAACCTGTAT CGGTAGCTGA ACCTGTATCG 
GTAGCTGAAC CTGAATCGGT AGCTGAACCT GAATCTGTAG CTGCATCTGT ACCTGTACCT 
GAATCTGTAC CTGAACCTGA ATCTGTACCT GAACCTGAAT CTGTACCTGA ACCTGAATCT 
GTACCTGAAC CTGAATCTGT ACCTGAACCT GAATCTGTAC CTGAACCTGA ATCTGTACCT 
GAACCTGAAT CTGTACCTGA ACCTGAATCT GTACCTGAAC CTGAATCAAT AGCTGAACCT 
GAATCAATAG CTGAACCTGA ATCTGTACCT GTACCTGAAT CTGTACCTGT ACCTGAATCT 
GTACCTGTTG CCACAAGGCC AGCACCAGTA GAGGTGACAG TAGAGGAAGC TCCAATACAG 
AGGGGGCAAA GGAGGGCCAG AAGCAGGAGT CCGGATCAGC GCAGGACGCG AGCTAGAACC 
GATAGAAGCA GATCCCCTCT ATATCAAACT GTTGATCCAC CTATTCGAAG GGCTCAGCAT 
AGTTCATCAC AGACTGTTGA TGCTTCCAAT ACAGAAGAAG CAGAGGGTAG CTCTAGGACT 
AGGCACCATG TTAGTTCTCA AGTGCACAGC TCTTCATCAA ATGAAACAGA GGGTAGTTCT 
AGAACTAGAC AGCATATTAC AGCCAGACAA CAAGCTCTTG GGACTGAAGG TCAGAGCCAA 
AGCACTGTTC ATCTGTCAAA CCCCGAGTCA AGAAGCTCTT CCCAAACTCC TCAGACAGAC 
TCCCCCAGTA ATGCAGAGAC TACAGGTACC GGGCAGAGGC CTCCCACCAT TGTGCTAGAT 
CTGCAAGTAA GGCGCGTGCG CCCAGGTGAT TACAGGCAGA GAGACAGCAT AGCTAACCGG 
ACACGTTCAC GCTCTCAGAC TCCCAACAAC ACTGTCACAT ATGAGAGCGA GAGAGGAGGA 
TTTCGTCGCA CTTTCTCGCG GTCTGAACGT GCCGGGGTTA GAACCTATGT AAGTACCATC 
CGAATTCCAA TCCGAAGAAT CCTGAACACT GGGCTCAGTG AGACCACATC TGTAGCCATC 
CAGACAATGC TGAGGCAAAT AATGACTGGC TTTGGGGAAC TCAGCTACTT CATGTACAAT 
GATAACGACG CTGATCCTAA CAACCCCACA GCAGTATCTC CTCCTGCAGC AGTTCCTGGT 
GAAGTACAAA ACAACCCAAG CGCAGAGGTC AGAGCTCCTA TTGCTGAGCC TGCAGAACCT 
GTTGCTCCAG TGGAAAGTGA TGAAGGCAGC AATGTTGCAA CATCTGCAAC CAGGAGGGAG 
GGTCGCAATA GCAGAGGTGG TGTCACTTTG GAAGAAAGCG GATCTCTACC TTTTCTTAGT 
CTGGCCCAGT TTTTCCTGCT TAATGAGGAT GATGATGATC AGCCAAGAGG ACTCACCAAA 
GAGCAAATCG ACAACCTGTC CACCCGAAAT TATGGTGAAA ATGATGCCCT GAAAACCTGT 
AGCGTGTGCA TAACAGAATA TACAGAAGGG AACAAGCTGC GCAAACTGCC TTGTTCCCAC 
GAGTACCACA TCCACTGTAT TGACCGCTGG TTGTCCGAAA ATTCTACCTG TCCCATCTGT 
CGCCGAGCAG TCCTGGTTGC AGGCAACAGA GAGAGCATTG TGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_018084005.1
CDS	336..2660
Translation	MWKLTVAKRDSRWTTSSLYPFIIMESADSTGKGSTEQSESQRQSQMDRLDREEAFYQFVNNLNDEDYRLMRDNNLLGTPGEITKEELLQRLQQIKEGPPQPSTEETRGDSVSTGEDPAEDSSNGDSIIDWLNSVRQTGNTTRSGQRGNQSWRAVSRTNPNSGDFRFSLEINVNRTSGTASMPSLDQSAEMPGPEDMEVSSQGEAENVPEPETVPESIREPESVDEPVSVAEPVSVAEPVSVAEPESVAEPESVAASVPVPESVPEPESVPEPESVPEPESVPEPESVPEPESVPEPESVPEPESVPEPESVPEPESIAEPESIAEPESVPVPESVPVPESVPVATRPAPVEVTVEEAPIQRGQRRARSRSPDQRRTRARTDRSRSPLYQTVDPPIRRAQHSSSQTVDASNTEEAEGSSRTRHHVSSQVHSSSSNETEGSSRTRQHITARQQALGTEGQSQSTVHLSNPESRSSSQTPQTDSPSNAETTGTGQRPPTIVLDLQVRRVRPGDYRQRDSIANRTRSRSQTPNNTVTYESERGGFRRTFSRSERAGVRTYVSTIRIPIRRILNTGLSETTSVAIQTMLRQIMTGFGELSYFMYNDNDADPNNPTAVSPPAAVPGEVQNNPSAEVRAPIAEPAEPVAPVESDEGSNVATSATRREGRNSRGGVTLEESGSLPFLSLAQFFLLNEDDDDQPRGLTKEQIDNLSTRNYGENDALKTCSVCITEYTEGNKLRKLPCSHEYHIHCIDRWLSENSTCPICRRAVLVAGNRESIV

Target ORF information:

RefSeq Version	XM_018228516.1
Organism	Xenopus laevis(African clawed frog)
Definition	Xenopus laevis ring finger protein, LIM domain interacting S homeolog (rlim.S), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_018228516.1

ORF Insert Sequence:

ATGTGGAAGC TGACGGTAGC GAAGCGGGAT TCACGTTGGA CAACATCATC ATTGTATCCA 
TTCATCATCA TGGAGAGTGC GGATTCCACC GGAAAAGGGA GCACTGAACA GTCCGAGTCT 
CAGCGCCAAA GCCAGATGGA CAGGCTAGAT CGAGAGGAAG CTTTTTATCA GTTTGTAAAT 
AACTTAAATG ATGAGGACTA CAGACTAATG AGAGATAACA ACTTATTGGG GACTCCAGGG 
GAAATTACTA AAGAGGAATT GTTGCAAAGG CTTCAGCAAA TCAAAGAAGG GCCACCACAG 
CCAAGTACAG AAGAAACAAG AGGCGATTCT GTCTCCACAG GTGAGGACCC TGCAGAGGAT 
TCATCCAATG GAGATTCCAT AATAGACTGG CTGAATTCAG TGCGACAGAC TGGGAACACA 
ACACGAAGTG GTCAGAGAGG CAATCAGTCC TGGAGGGCAG TGAGCAGGAC AAATCCAAAC 
AGTGGGGATT TCAGGTTTAG TTTGGAAATC AATGTGAATC GTACTTCTGG CACCGCAAGC 
ATGCCTTCGC TTGACCAATC GGCTGAAATG CCTGGGCCTG AGGATATGGA AGTGAGTAGT 
CAGGGTGAGG CTGAGAATGT ACCTGAACCT GAAACTGTAC CTGAATCTAT ACGTGAACCT 
GAATCTGTAG ATGAACCTGT ATCGGTAGCT GAACCTGTAT CGGTAGCTGA ACCTGTATCG 
GTAGCTGAAC CTGAATCGGT AGCTGAACCT GAATCTGTAG CTGCATCTGT ACCTGTACCT 
GAATCTGTAC CTGAACCTGA ATCTGTACCT GAACCTGAAT CTGTACCTGA ACCTGAATCT 
GTACCTGAAC CTGAATCTGT ACCTGAACCT GAATCTGTAC CTGAACCTGA ATCTGTACCT 
GAACCTGAAT CTGTACCTGA ACCTGAATCT GTACCTGAAC CTGAATCAAT AGCTGAACCT 
GAATCAATAG CTGAACCTGA ATCTGTACCT GTACCTGAAT CTGTACCTGT ACCTGAATCT 
GTACCTGTTG CCACAAGGCC AGCACCAGTA GAGGTGACAG TAGAGGAAGC TCCAATACAG 
AGGGGGCAAA GGAGGGCCAG AAGCAGGAGT CCGGATCAGC GCAGGACGCG AGCTAGAACC 
GATAGAAGCA GATCCCCTCT ATATCAAACT GTTGATCCAC CTATTCGAAG GGCTCAGCAT 
AGTTCATCAC AGACTGTTGA TGCTTCCAAT ACAGAAGAAG CAGAGGGTAG CTCTAGGACT 
AGGCACCATG TTAGTTCTCA AGTGCACAGC TCTTCATCAA ATGAAACAGA GGGTAGTTCT 
AGAACTAGAC AGCATATTAC AGCCAGACAA CAAGCTCTTG GGACTGAAGG TCAGAGCCAA 
AGCACTGTTC ATCTGTCAAA CCCCGAGTCA AGAAGCTCTT CCCAAACTCC TCAGACAGAC 
TCCCCCAGTA ATGCAGAGAC TACAGGTACC GGGCAGAGGC CTCCCACCAT TGTGCTAGAT 
CTGCAAGTAA GGCGCGTGCG CCCAGGTGAT TACAGGCAGA GAGACAGCAT AGCTAACCGG 
ACACGTTCAC GCTCTCAGAC TCCCAACAAC ACTGTCACAT ATGAGAGCGA GAGAGGAGGA 
TTTCGTCGCA CTTTCTCGCG GTCTGAACGT GCCGGGGTTA GAACCTATGT AAGTACCATC 
CGAATTCCAA TCCGAAGAAT CCTGAACACT GGGCTCAGTG AGACCACATC TGTAGCCATC 
CAGACAATGC TGAGGCAAAT AATGACTGGC TTTGGGGAAC TCAGCTACTT CATGTACAAT 
GATAACGACG CTGATCCTAA CAACCCCACA GCAGTATCTC CTCCTGCAGC AGTTCCTGGT 
GAAGTACAAA ACAACCCAAG CGCAGAGGTC AGAGCTCCTA TTGCTGAGCC TGCAGAACCT 
GTTGCTCCAG TGGAAAGTGA TGAAGGCAGC AATGTTGCAA CATCTGCAAC CAGGAGGGAG 
GGTCGCAATA GCAGAGGTGG TGTCACTTTG GAAGAAAGCG GATCTCTACC TTTTCTTAGT 
CTGGCCCAGT TTTTCCTGCT TAATGAGGAT GATGATGATC AGCCAAGAGG ACTCACCAAA 
GAGCAAATCG ACAACCTGTC CACCCGAAAT TATGGTGAAA ATGATGCCCT GAAAACCTGT 
AGCGTGTGCA TAACAGAATA TACAGAAGGG AACAAGCTGC GCAAACTGCC TTGTTCCCAC 
GAGTACCACA TCCACTGTAT TGACCGCTGG TTGTCCGAAA ATTCTACCTG TCCCATCTGT 
CGCCGAGCAG TCCTGGTTGC AGGCAACAGA GAGAGCATTG TGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

rlim.S ( XM_018228517.1 ) cDNA ORF clone, Xenopus laevis(African clawed frog) -> XP_018084006.1 Xenopus laevis ring finger protein, LIM domain interacting S homeolog (rlim.S), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OAf40888
Clone ID Related Accession (Same CDS sequence)	XM_018228517.1
Accession Version	XM_018228517.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2307bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1474214400000
Organism	Xenopus laevis(African clawed frog)
Product	E3 ubiquitin-protein ligase RNF12-B isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030738.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus laevis Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGGAAGC TGACGGTAGC GAAGCGGGAT TCACGTTGGA CAACATCATC ATTGTATCCA 
TTCATCATCA TGGAGAGTGC GGATTCCACC GGAAAAGGGA GCACTGAACA GTCCGAGTCT 
CAGCGCCAAA GCCAGATGGA CAGGCTAGAT CGAGAGGAAG CTTTTTATCA GTTTGTAAAT 
AACTTAAATG ATGAGGACTA CAGACTAATG AGAGATAACA ACTTATTGGG GACTCCAGGG 
GAAATTACTA AAGAGGAATT GTTGCAAAGG CTTCAGCAAA TCAAAGAAGG GCCACCACAG 
CCAAGTACAG AAGAAACAAG AGGTGAGGAC CCTGCAGAGG ATTCATCCAA TGGAGATTCC 
ATAATAGACT GGCTGAATTC AGTGCGACAG ACTGGGAACA CAACACGAAG TGGTCAGAGA 
GGCAATCAGT CCTGGAGGGC AGTGAGCAGG ACAAATCCAA ACAGTGGGGA TTTCAGGTTT 
AGTTTGGAAA TCAATGTGAA TCGTACTTCT GGCACCGCAA GCATGCCTTC GCTTGACCAA 
TCGGCTGAAA TGCCTGGGCC TGAGGATATG GAAGTGAGTA GTCAGGGTGA GGCTGAGAAT 
GTACCTGAAC CTGAAACTGT ACCTGAATCT ATACGTGAAC CTGAATCTGT AGATGAACCT 
GTATCGGTAG CTGAACCTGT ATCGGTAGCT GAACCTGTAT CGGTAGCTGA ACCTGAATCG 
GTAGCTGAAC CTGAATCTGT AGCTGCATCT GTACCTGTAC CTGAATCTGT ACCTGAACCT 
GAATCTGTAC CTGAACCTGA ATCTGTACCT GAACCTGAAT CTGTACCTGA ACCTGAATCT 
GTACCTGAAC CTGAATCTGT ACCTGAACCT GAATCTGTAC CTGAACCTGA ATCTGTACCT 
GAACCTGAAT CTGTACCTGA ACCTGAATCA ATAGCTGAAC CTGAATCAAT AGCTGAACCT 
GAATCTGTAC CTGTACCTGA ATCTGTACCT GTACCTGAAT CTGTACCTGT TGCCACAAGG 
CCAGCACCAG TAGAGGTGAC AGTAGAGGAA GCTCCAATAC AGAGGGGGCA AAGGAGGGCC 
AGAAGCAGGA GTCCGGATCA GCGCAGGACG CGAGCTAGAA CCGATAGAAG CAGATCCCCT 
CTATATCAAA CTGTTGATCC ACCTATTCGA AGGGCTCAGC ATAGTTCATC ACAGACTGTT 
GATGCTTCCA ATACAGAAGA AGCAGAGGGT AGCTCTAGGA CTAGGCACCA TGTTAGTTCT 
CAAGTGCACA GCTCTTCATC AAATGAAACA GAGGGTAGTT CTAGAACTAG ACAGCATATT 
ACAGCCAGAC AACAAGCTCT TGGGACTGAA GGTCAGAGCC AAAGCACTGT TCATCTGTCA 
AACCCCGAGT CAAGAAGCTC TTCCCAAACT CCTCAGACAG ACTCCCCCAG TAATGCAGAG 
ACTACAGGTA CCGGGCAGAG GCCTCCCACC ATTGTGCTAG ATCTGCAAGT AAGGCGCGTG 
CGCCCAGGTG ATTACAGGCA GAGAGACAGC ATAGCTAACC GGACACGTTC ACGCTCTCAG 
ACTCCCAACA ACACTGTCAC ATATGAGAGC GAGAGAGGAG GATTTCGTCG CACTTTCTCG 
CGGTCTGAAC GTGCCGGGGT TAGAACCTAT GTAAGTACCA TCCGAATTCC AATCCGAAGA 
ATCCTGAACA CTGGGCTCAG TGAGACCACA TCTGTAGCCA TCCAGACAAT GCTGAGGCAA 
ATAATGACTG GCTTTGGGGA ACTCAGCTAC TTCATGTACA ATGATAACGA CGCTGATCCT 
AACAACCCCA CAGCAGTATC TCCTCCTGCA GCAGTTCCTG GTGAAGTACA AAACAACCCA 
AGCGCAGAGG TCAGAGCTCC TATTGCTGAG CCTGCAGAAC CTGTTGCTCC AGTGGAAAGT 
GATGAAGGCA GCAATGTTGC AACATCTGCA ACCAGGAGGG AGGGTCGCAA TAGCAGAGGT 
GGTGTCACTT TGGAAGAAAG CGGATCTCTA CCTTTTCTTA GTCTGGCCCA GTTTTTCCTG 
CTTAATGAGG ATGATGATGA TCAGCCAAGA GGACTCACCA AAGAGCAAAT CGACAACCTG 
TCCACCCGAA ATTATGGTGA AAATGATGCC CTGAAAACCT GTAGCGTGTG CATAACAGAA 
TATACAGAAG GGAACAAGCT GCGCAAACTG CCTTGTTCCC ACGAGTACCA CATCCACTGT 
ATTGACCGCT GGTTGTCCGA AAATTCTACC TGTCCCATCT GTCGCCGAGC AGTCCTGGTT 
GCAGGCAACA GAGAGAGCAT TGTGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_018084006.1
CDS	338..2644
Translation	MWKLTVAKRDSRWTTSSLYPFIIMESADSTGKGSTEQSESQRQSQMDRLDREEAFYQFVNNLNDEDYRLMRDNNLLGTPGEITKEELLQRLQQIKEGPPQPSTEETRGEDPAEDSSNGDSIIDWLNSVRQTGNTTRSGQRGNQSWRAVSRTNPNSGDFRFSLEINVNRTSGTASMPSLDQSAEMPGPEDMEVSSQGEAENVPEPETVPESIREPESVDEPVSVAEPVSVAEPVSVAEPESVAEPESVAASVPVPESVPEPESVPEPESVPEPESVPEPESVPEPESVPEPESVPEPESVPEPESVPEPESIAEPESIAEPESVPVPESVPVPESVPVATRPAPVEVTVEEAPIQRGQRRARSRSPDQRRTRARTDRSRSPLYQTVDPPIRRAQHSSSQTVDASNTEEAEGSSRTRHHVSSQVHSSSSNETEGSSRTRQHITARQQALGTEGQSQSTVHLSNPESRSSSQTPQTDSPSNAETTGTGQRPPTIVLDLQVRRVRPGDYRQRDSIANRTRSRSQTPNNTVTYESERGGFRRTFSRSERAGVRTYVSTIRIPIRRILNTGLSETTSVAIQTMLRQIMTGFGELSYFMYNDNDADPNNPTAVSPPAAVPGEVQNNPSAEVRAPIAEPAEPVAPVESDEGSNVATSATRREGRNSRGGVTLEESGSLPFLSLAQFFLLNEDDDDQPRGLTKEQIDNLSTRNYGENDALKTCSVCITEYTEGNKLRKLPCSHEYHIHCIDRWLSENSTCPICRRAVLVAGNRESIV

Target ORF information:

RefSeq Version	XM_018228517.1
Organism	Xenopus laevis(African clawed frog)
Definition	Xenopus laevis ring finger protein, LIM domain interacting S homeolog (rlim.S), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_018228517.1

ORF Insert Sequence:

ATGTGGAAGC TGACGGTAGC GAAGCGGGAT TCACGTTGGA CAACATCATC ATTGTATCCA 
TTCATCATCA TGGAGAGTGC GGATTCCACC GGAAAAGGGA GCACTGAACA GTCCGAGTCT 
CAGCGCCAAA GCCAGATGGA CAGGCTAGAT CGAGAGGAAG CTTTTTATCA GTTTGTAAAT 
AACTTAAATG ATGAGGACTA CAGACTAATG AGAGATAACA ACTTATTGGG GACTCCAGGG 
GAAATTACTA AAGAGGAATT GTTGCAAAGG CTTCAGCAAA TCAAAGAAGG GCCACCACAG 
CCAAGTACAG AAGAAACAAG AGGTGAGGAC CCTGCAGAGG ATTCATCCAA TGGAGATTCC 
ATAATAGACT GGCTGAATTC AGTGCGACAG ACTGGGAACA CAACACGAAG TGGTCAGAGA 
GGCAATCAGT CCTGGAGGGC AGTGAGCAGG ACAAATCCAA ACAGTGGGGA TTTCAGGTTT 
AGTTTGGAAA TCAATGTGAA TCGTACTTCT GGCACCGCAA GCATGCCTTC GCTTGACCAA 
TCGGCTGAAA TGCCTGGGCC TGAGGATATG GAAGTGAGTA GTCAGGGTGA GGCTGAGAAT 
GTACCTGAAC CTGAAACTGT ACCTGAATCT ATACGTGAAC CTGAATCTGT AGATGAACCT 
GTATCGGTAG CTGAACCTGT ATCGGTAGCT GAACCTGTAT CGGTAGCTGA ACCTGAATCG 
GTAGCTGAAC CTGAATCTGT AGCTGCATCT GTACCTGTAC CTGAATCTGT ACCTGAACCT 
GAATCTGTAC CTGAACCTGA ATCTGTACCT GAACCTGAAT CTGTACCTGA ACCTGAATCT 
GTACCTGAAC CTGAATCTGT ACCTGAACCT GAATCTGTAC CTGAACCTGA ATCTGTACCT 
GAACCTGAAT CTGTACCTGA ACCTGAATCA ATAGCTGAAC CTGAATCAAT AGCTGAACCT 
GAATCTGTAC CTGTACCTGA ATCTGTACCT GTACCTGAAT CTGTACCTGT TGCCACAAGG 
CCAGCACCAG TAGAGGTGAC AGTAGAGGAA GCTCCAATAC AGAGGGGGCA AAGGAGGGCC 
AGAAGCAGGA GTCCGGATCA GCGCAGGACG CGAGCTAGAA CCGATAGAAG CAGATCCCCT 
CTATATCAAA CTGTTGATCC ACCTATTCGA AGGGCTCAGC ATAGTTCATC ACAGACTGTT 
GATGCTTCCA ATACAGAAGA AGCAGAGGGT AGCTCTAGGA CTAGGCACCA TGTTAGTTCT 
CAAGTGCACA GCTCTTCATC AAATGAAACA GAGGGTAGTT CTAGAACTAG ACAGCATATT 
ACAGCCAGAC AACAAGCTCT TGGGACTGAA GGTCAGAGCC AAAGCACTGT TCATCTGTCA 
AACCCCGAGT CAAGAAGCTC TTCCCAAACT CCTCAGACAG ACTCCCCCAG TAATGCAGAG 
ACTACAGGTA CCGGGCAGAG GCCTCCCACC ATTGTGCTAG ATCTGCAAGT AAGGCGCGTG 
CGCCCAGGTG ATTACAGGCA GAGAGACAGC ATAGCTAACC GGACACGTTC ACGCTCTCAG 
ACTCCCAACA ACACTGTCAC ATATGAGAGC GAGAGAGGAG GATTTCGTCG CACTTTCTCG 
CGGTCTGAAC GTGCCGGGGT TAGAACCTAT GTAAGTACCA TCCGAATTCC AATCCGAAGA 
ATCCTGAACA CTGGGCTCAG TGAGACCACA TCTGTAGCCA TCCAGACAAT GCTGAGGCAA 
ATAATGACTG GCTTTGGGGA ACTCAGCTAC TTCATGTACA ATGATAACGA CGCTGATCCT 
AACAACCCCA CAGCAGTATC TCCTCCTGCA GCAGTTCCTG GTGAAGTACA AAACAACCCA 
AGCGCAGAGG TCAGAGCTCC TATTGCTGAG CCTGCAGAAC CTGTTGCTCC AGTGGAAAGT 
GATGAAGGCA GCAATGTTGC AACATCTGCA ACCAGGAGGG AGGGTCGCAA TAGCAGAGGT 
GGTGTCACTT TGGAAGAAAG CGGATCTCTA CCTTTTCTTA GTCTGGCCCA GTTTTTCCTG 
CTTAATGAGG ATGATGATGA TCAGCCAAGA GGACTCACCA AAGAGCAAAT CGACAACCTG 
TCCACCCGAA ATTATGGTGA AAATGATGCC CTGAAAACCT GTAGCGTGTG CATAACAGAA 
TATACAGAAG GGAACAAGCT GCGCAAACTG CCTTGTTCCC ACGAGTACCA CATCCACTGT 
ATTGACCGCT GGTTGTCCGA AAATTCTACC TGTCCCATCT GTCGCCGAGC AGTCCTGGTT 
GCAGGCAACA GAGAGAGCAT TGTGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Selective degradation of excess Ldb1 by Rnf12/RLIM confers proper Ldb1 expression levels and Xlim-1/Ldb1 stoichiometry in Xenopus organizer functions.
Development (Cambridge, England)130(17)4161-75(2003 Sep)
Hiratani I,Yamamoto N,Mochizuki T,Ohmori SY,Taira M

Genetic and genomic tools for Xenopus research: The NIH Xenopus initiative.
Developmental dynamics : an official publication of the American Association of Anatomists225(4)384-91(2002 Dec)
Klein SL,Strausberg RL,Wagner L,Pontius J,Clifton SW,Richardson P

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rlim.S cDNA ORF clone, Xenopus laevis(African clawed frog)

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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