LOC100037049 cDNA ORF clone, Xenopus laevis(African clawed frog)

Gene
Clones
gRNAs

Summary

Gene Symbol	LOC100037049
Entrez Gene ID	100037049
Full Name	SEC16 homolog A, endoplasmic reticulum export factor S homeolog
Synonyms	sec16l
General protein information	Preferred Names SEC16 homolog A, endoplasmic reticulum export factor S homeolog Names protein transport protein Sec16A
Gene Type	protein-coding
Organism	Xenopus laevis(African clawed frog)

mRNA and Protein(s)

mRNA	Protein	Name
XM_018232582.1	XP_018088071.1	protein transport protein Sec16A isoform X4
XM_018232579.1	XP_018088068.1	protein transport protein Sec16A isoform X1
XM_018232581.1	XP_018088070.1	protein transport protein Sec16A isoform X3
XM_018232580.1	XP_018088069.1	protein transport protein Sec16A isoform X2

Pathways from BioSystems
Gene Ontology

The following LOC100037049 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC100037049 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OAf43839	XM_018232579.1 Latest version!	Xenopus laevis protein transport protein Sec16A (LOC100037049), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1301.30 $1859.00
OAf43840	XM_018232580.1 Latest version!	Xenopus laevis protein transport protein Sec16A (LOC100037049), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1301.30 $1859.00
OAf43841	XM_018232581.1 Latest version!	Xenopus laevis protein transport protein Sec16A (LOC100037049), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1301.30 $1859.00
OAf43842	XM_018232582.1 Latest version!	Xenopus laevis protein transport protein Sec16A (LOC100037049), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1301.30 $1859.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LOC100037049 ( XM_018232579.1 ) cDNA ORF clone, Xenopus laevis(African clawed frog) -> XP_018088068.1 Xenopus laevis protein transport protein Sec16A (LOC100037049), transcript variant X1, mRNA.

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CloneID	OAf43839
Clone ID Related Accession (Same CDS sequence)	XM_018232579.1
Accession Version	XM_018232579.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7095bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1474214400000
Organism	Xenopus laevis(African clawed frog)
Product	protein transport protein Sec16A isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030739.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus laevis Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAGCCCC CTCCGCAAGC AAGGCATCCT GGCACGGGTG GACCTCCTCG CATGGGACTT 
GGCAATAATG CCCACTGGCG CAAAAGGGGA CCTAATCCTG TACCAGTAGC TTCAGCTTCA 
GTGCAGCCAG TAACAGACCC TTTTGCCTTT GGCCGTATTA CCCCACAAAG TATGGCACAG 
GGCAATGTCC CGATAGGAAA CCCACTGATG GCACCCAGTG TTTCCCCTTC TTTTTACCCT 
CAACCCGTTC CTGTACATGC ATCGGCTGCT GTAGATCAAG GGCATGGGTC TCCTGCATCA 
CTACCAGGAT TTGTTCCACA ACCTGCTGCT ACGGGGGGGA TGTATACAAA TGTGTCTGTC 
CCATCACCAT CAAGTGCCCC CTTTCACAAC TACCCAGCCG CCACCTCTAG TGCTCAGCCT 
GGACTCAGTA ATATTCAGAC TTTTCAGAAT ACAGCAAGTG GACTTGAAAA TGCCTTTCTG 
CCAGTGCACA TCAGGGCTCA GTCAACACAA GGCATTAATT ATAACCCTCC ATCTTCATCA 
CCTTTGCAGC ACTTTATCCC TGCACCATCT CAGCCAACTA ATGCTCCCTC TGGGTCCTTA 
CAAAGTGTAC CTCCTGCCTC AGCTCAGAAC TTCCCCCTTC CTGTTCAACA GCCATCCCAT 
AATGCTCCTT ATTACTCCCC ACCTTATTTA GAAGTGTCTA ATCTGGTCCC TCAAGCCAAC 
GAAACAGTGG CACAGCAGAG GTCAGCTCCT GGGCATTACC CGCCTGCCAC TGTCGCCAAT 
CCTACATTTC AACACAGCAC TTCACAACCC ACCATTTTAA ATCCGATAGA CTATATGCAC 
CGCCACAATC CTGACCAGAA CTGGGTTAAG CAACCTCACA AGGATCAGCA GCATCCACCT 
TTTCCCAGTC CTAACCCCGT ACAATACCCA ACTCAGCCAC CTGAGCCCCA TCAGGAGGTG 
ACTAATGCAT CTGGTCATGT CTCCACCAAT GTCGACTCTG GTACTATATC TATGTTTTTT 
AAAGGGGATG AAGAAGAAAA TGTAGAAATA GTTTCTTCCG ACCAGCAGAA TGCATCTGTT 
AAAGTGGATC ATGGTTCTTC TCATTACCAG AACTCTGGAC ATGAAGGTGT GCCTTCTCTC 
CTGCCACAGC CCACAGTCAG CAGCATGTCG TCACTTACAC CAATCTCTGT GTCTCAGAAT 
GTTGGCAATT TGACCCAACC ACATTCAGAT TGGAGGCAGG ATGTGTTTCC TGTTGGCGAT 
AAAATTTCTA AACCCAAAGT TGGAAGTGAA TTGCCCTATG ACAATGTTGA AAACATAGAG 
TGTGTACAAA ACCAAGAGGT TCCCCCCAAC GAGCCCAATC ATTTTAACCT TTATCCTTCT 
AATGTAAATT CAGATACGTA CCGCTATGGG TCCATGCCTG GACTCTCCCT ACAAAAGAAT 
GTTAGCAGCC AACCTGAAGC TGGTCCGAAT CTAGAGACGC CTGTCACAGT GGGTTGTCCT 
GTCAGATCTG ATAGTGTTTC ATCAAATTAT AGCAACCTGA GTCACAAAAG CCTGCCTGTG 
CCCACCCGGC CTCAAGAGCT AGCCGGGACA TTTATTCAGA AGGAGAGAGG AAGGCTTGAA 
GGTGACTCCT CCCATGGTTT CTTTAAGCAA ATTGATTCTT TGCCCATGGC AGGTGATGCT 
GTAGGCCAGA ATATGGGTCA GCACACGTTC CACAGCAATT TATCCCAGCC ACCAACTCCA 
AGTCCTCCTA AACCTACTGG GATCTTCATG ACGAGTGCAA ATAGTTCTTT CGAACCTGTT 
AGATCGCACA GCATTGGTTT GAAAGCGTCC GACGTAGACC AATTGAAAAT GGGTGGAGAC 
TTAAGAGAGG GGCATAATGT TCCACCAAGC GCCTCTCTCC CAGCAGCTTC CCCTGGTAAC 
TTGGAACAAC CACCAGATAA CCTGGAATCC ATATTCTTAG GTAAAAGAAA TTCTCACGGA 
AACATTACCA GCAATGGGAA CTTGTTCCAT TCTGCTGGCG ACCCGCCATT GGATAATATA 
TCCTTACTGC TTGAGAGACG TCCATCGTCA AGAGCACACG GGTCTAACAA GAAATGCGAA 
AGCCCGGCAA CCACCCTGTG GGCTCAAGGT GAGCTTCCAA ATTTTGGCGG AAATGTTTTG 
TTGGCTCCTG CAGCTCCTTC ACTCTATGTG CCCCCTAAAC CCCAAACCGA AATAATTCAG 
CCACCTGAAG ATGGGTCTTA TGATTCATGC TCGGGTGGAA CAGATATGGG GAAATTTCAG 
ACCTCTCTGG AAGAACAAAT TTCTTCGGAA AACCTTGAGA ATCCTCCAAA GATCACTGAT 
GAGGAGCAAC TTCCCTCGCA GGCAAGTTCA GGTTACGCCA GTTTGTTGTC TTCGCCGCCC 
ACTGAGGTAC TTCAGAATCA GCCCTTGCTA ATTGCCCAAC CAAATAAAAC CTATAATTTG 
TCACAGCCTA ATAATTTTTC ATTGTCGTTA CCAAATAAGG TAAAAAATAT TAATTCGCTT 
AAGGAACTGG GAGCAGCTGA CAAGCCGGGT CCTCAGAGTT TGCCTCCAGC TGTGGGGAAT 
TTTAACTCCA ATGACAATAC TGTGTTTTCC CTAATTCAAG TCCCACCCGA GGCTTCTAAT 
CCACCTAATT ACAAATCTGC TGCTACTAAT GATAAATTCT CTGAATCTCT TCACAATCTG 
AGTTCCATTT TGCAGCGGCA ATCATCAGCA AATCAAATGC CCCTCAATTT AGCTGCGGAA 
AGTCAAAAGG CACTTAAGGG TGAAAACGCG ACTGTCGATC TTGCAAATAA AAACCCGATT 
CCAGCTGCCC TTCCGACGGG GCCACGTTTC CCTGCCAATC CAAACGGCAA GCCAAATTAT 
CATGCAGAGA AGACCGTCGC TCTAGATTTT ACTGTCCCTC GTGCGCCAGT CAGTTCTGTT 
GCCAATAACG TTGCTTCAAG TAACAGTCAG CCGGTTGCTG GAGGGCCGCT CTTTCCTAGG 
CCGTCTGTCA GCTATCAGAA TGGTCAGGCA GGCCTAGATG TTAACAAGCA GGGTTTTTAT 
CAGCAGGTCA CTAAGGATGG GCCCCCGCAG CAGCAGCCTG TTATGGATAT CCAACACCAG 
GGAGGAGTGG GCCTGGGGGA ATACATGGTC CCATCCTCTC AGCCACTGTC CTTGGTTCTG 
CCTGCACAAG CGCCTCCAGT GGGTAAATCG GTTCCATACC CTCCACAAGC TGAAAGCCAA 
GTTCAAACCT TGCAGAACCC TAGTGGAACT CCTTATAACA GTGCGCCAGA ACCTGCACCT 
AAATACGGAC CAACTGAACA GCCTTCATAT CCAGCTCCGC CTGTTCCTTC CTCATCAGCC 
ACAGTGAACC ACATAGCAAC ATATCAAGAG CAGCCCAGGC CTCCTGCCTC CCATACCCCT 
CAGCAAGCTG ATCCTTATTA CTACAGGTCT TACGGTGCCT ACCCACAGCC TTACCAACCC 
TTGGACCCAA GGGCAGCACC TCTTTACTAT CCGGACCCTT ATGGCGCTTA TGACCCCACC 
TACAGGCAGT ACAGTGGGTC TGCATATGTG AACCCTGGCA ATTACTCCTA TCAGGAGCCC 
GAACGTCCCA GTTCCAGATC CAGCCACTGC TCCGATCGCC CCTCCTCAAG GCAGGGCTAT 
AGCAGTAAAG GAGACTGGAA TGCACACAGT GGTTACTACT CAGGGTATTA TCAGAGTCAG 
TATGATTATG CAGACCCCAG CCGCTGGGAC CGATATCCTG GAGCCTATGA CCCTGGCTAT 
AGAGACCCCA GCAGTAATTA CTGGAATTAT ATGTACAACA GCCGTGATGA TGTGTACCAA 
AGGAACGTGC CTACTGCTTA TACCACCAGA TATGACGTTT ATGAAGACCG CTGGCAATAT 
GACCCACGTT ATGTGGGAGG CTTTGATGAA GAGCCACAGA GAGACCTCAC GAAGGACGAC 
TTTGACCGAC GTAGTGTTCA CAGTGAGCAT TCGGTCGATA GCATGCACAG CGGCCGGAGT 
GCACACAGTC GGAGAAGCAG TTTCAGCTCC CATTCACAAC ATAGCCAGAT CTACAAAAGC 
CAGCCAGACC TGTCTGCCAA CGCGTATGAA CCCCAAATAC AGAATCCTCC TCTCAATGAG 
TATTCGTATG GCGTGTACCC CAATGACTAC GGCACCCAGC AGGCGCTTGA TGATTACTCT 
TACGGGTATC CCAAGGGCAC AGAGTGGCAA CCTGTAGAAC AAGTGCCTTC AAGGCCGTTG 
ACTCCAGAGA AGTTTGGGAG CCCACACATC TGTGCCAGGT TTGGTTCTGG GGGTCAGTTA 
ATAAAGGTGC TGCCTAACCT TCCTTCAGAC GGACAGCCGG CCTTAGTGGA AATCCACAAC 
ATGGAGGTCA TTATGCAGAA CAGCGCAGAG CAGGAACAGC TGAGAAGTTT CCCTGGACCT 
TTAGTGAAGG ATGAAACTCA TAAAGTTGAC GTCATCAGTT TTGCCCAGAA GAAATTCAAG 
GAGTGTTCTC AGAATGAGAG CCTGTTAGAC CAAGAGTCTG CTTGCCTGCT CTGGGACTTC 
ATTGTGCTTC TGTGCCGGCA AAATGGGACT GTTGTTGGAA CAGATATCGC TGAGCTGCTT 
TTGAGAGATC ACAAGACAGT GTGGCTTCCT GCAAAGTCTC CCAACGAAGC CAACCTGATC 
GATTTCAGCA ATGAGCCGCT CGAGCAGCAG GAGGAAGAGT CAGGGGCTTC TCAGTTGTCC 
TTCCTCACGG ATTCTTTGCA AAGCACCGGG GAAGCTCTAG AGAAGGAGAC TGAGCGGTTC 
AGAGAGCTTC TACTTTTTGG GCGTAAAAAG GATGCCTTAG AATCTGCTAT GAAAAATGGA 
CTGTGGGGCC ACGCTTTGCT CCTAGCTAGC AAAATGGACA GCCGAACGCA CGCCAGAGTT 
ATGACAAGGT TTGCCAACAG CCTTCCAATT AACGATCCCC TGCAGACTGT GTATCAGCTC 
TTGTCTGGGA GAATGCCAGC AGCAGCCACG TGCTGCGGAG ATGAGAAATG GGGTGACTGG 
CGCCCTCACC TGGCGATGGT GCTGTCTAAT CTGACCAACA ATGTTGACGT TCCCACCAGG 
GCCATGACAA CAATGGGAGA TACATTAGCA TCCCGGGGGC TGATGGATGC CTCACACTTC 
TGCTATTTAA TGGCACAAGT CGGCTTTGGC ATTTTCACCA AGAAATCTAC TAAGTTGGTC 
CTCATTGGAT CCAACCACAG TTTATCATTT GCAAAGTTTT GCACTAACGA AGCCATCCAG 
AGAACAGAGG CTTATGAATA TGCACAGGCA CTTGGCACCC AGTCTTTCTC TCTCCCGAAT 
ATACAGGTTT ACAAGTTCAT CTACGCTTGC CGTTTGGCGG AACACGGATT ATCTGCACAA 
GCTTTCCATT ACTGTGAAGT CATCTCCAAG ACCATCTTGA AGCACCCGTC TTGCTACTCC 
CCTGTGCTTG TCAGCCAGCT ACTGGAGGTG TCCTCCCACT TGAGGTTCTT TGACCCACAG 
CTGAAGGAGA AGCCAGAACA GGAGTTATTT ATCGACCCTC CTTGGCTGCA GCATCTCCGC 
ACCTTGGATA AGCAAATGAA GCATGGTACT GTAGTTTATG ACATGGGCAG AACCACACCT 
CAGCAGTTTG CCTGCAGTAC ACCAAGCTCG GAGATAGACC ATGTGAGCCA ATCAGATGGT 
TTTGGAATAG CCGGGGATAT ACCAACTCAT ACAGAGAATC CATTACTGGC CAATCTCCTG 
CCCAACATGA TGCCTCCAGT ACAAGCTGTG CTAGTTCCTG CTGGTCCTGA AAATCCGCCA 
CCTGTATATC CACCCTACAC AGGGAATGAT CCCTCCTACC CAGGTACACC TCAGGGTCTA 
GCTCCCTCAT CTGGGCCTCC AACTCCATCT ACTGCTCCAA ATTTTGTGCC GTTTGGTGTG 
GAACAGGCAA CTGTTTATCC ACCTGCTGCT CTACAGCCAT ACCCACCTCT TCCCCAGGCA 
ACAGATTGTG CTCCTCCAAT GCAGATGAAT CAAGATCCAG GACTGCAAAG GATTCAGCAG 
AATTCCCCTA CCAAAACCAC CTTCGCTGAT CCTGAAGATT TCTACGGTGT CATGGCAAAA 
ATGGTTCCCG GGCAGAGATC ACGGACAGTG TCACAGTCCT CATCACACAT GAGGCGGACA 
CGGACAACCT CAGAGTCATC AACCCATTCT ATTGGGTCTG GACGTAGAAG TTCCATGGGC 
ATCCAACCAT CGCCTCCTCC TGCCTCTATC CCAGAGGCAG ACAGACCAGA AGAGAAGAAC 
AAACATAAGT CTCATGGTCC ATCCCAAGGG AGTGGTAAAA GTTGGTTTCC CCTGAAATTT 
GGCTGGTTGA TGGGAAAAGG CAAGAACGAA GCACACTTAC CTGATGACAG GAATAAATCT 
ATTGTGTGGG ATGAGAAGCA GCAGCGGTGG ACTAACCTGG ATGAACCAGA AGAAGAGAGA 
AAACCGTTGC CTCCACCACC ACCAGGGATG CTGAAAAAAC CACAGACTGC TCCATCTGTC 
CCAGGTGGTC CTCTCAACCC TTCTGTCAAT GTTTTCTCCA TCAGAGCAGC AGGGGCAAGA 
GGTCGTTACA TTGATGTCTT AAACCCAGGG GGTACCCGGC CCACTAACTC GGTCCCTCCG 
CCTGCTGATT TGTTTGCACC TTTGGCCCCC ATGCCTATCC CCACGAACCT GTTTGTTCCA 
AATGCAGTCC CAGAAGAGGG TCAGCCTGCG GAAGCCAGTG GGGCAGAAAT GGTGCAACCT 
GCGGGTCAGA CGGGGCTCAG TAACTTTTCC CAGGCACAGT TACCCGGTTT GCCCCCTGTG 
TCTGATCTTC CAACAAATCA GGAAGATGGG CAGATGGGAG AGCTTTCCCG ATCTAGCTCT 
CTCAGTTCCT TATCCCGTGA AGTAAGCCAG CATATGGAGC AGGCTCACGT GGCCCCTGCG 
TCAGGCCCCC CACCTACAGG GGCTGTCCCC TTCTACAACC CCTCCCAGTT TTCACAACAG 
CAGCCTCCTT CTGCCCAAGC AGGTGGGCTA CGACCTGGAC GTCTCGGCCA GAGGAAATAT 
CCTTCCTTAA AATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_018088068.1
CDS	215..7309
Translation	MQPPPQARHPGTGGPPRMGLGNNAHWRKRGPNPVPVASASVQPVTDPFAFGRITPQSMAQGNVPIGNPLMAPSVSPSFYPQPVPVHASAAVDQGHGSPASLPGFVPQPAATGGMYTNVSVPSPSSAPFHNYPAATSSAQPGLSNIQTFQNTASGLENAFLPVHIRAQSTQGINYNPPSSSPLQHFIPAPSQPTNAPSGSLQSVPPASAQNFPLPVQQPSHNAPYYSPPYLEVSNLVPQANETVAQQRSAPGHYPPATVANPTFQHSTSQPTILNPIDYMHRHNPDQNWVKQPHKDQQHPPFPSPNPVQYPTQPPEPHQEVTNASGHVSTNVDSGTISMFFKGDEEENVEIVSSDQQNASVKVDHGSSHYQNSGHEGVPSLLPQPTVSSMSSLTPISVSQNVGNLTQPHSDWRQDVFPVGDKISKPKVGSELPYDNVENIECVQNQEVPPNEPNHFNLYPSNVNSDTYRYGSMPGLSLQKNVSSQPEAGPNLETPVTVGCPVRSDSVSSNYSNLSHKSLPVPTRPQELAGTFIQKERGRLEGDSSHGFFKQIDSLPMAGDAVGQNMGQHTFHSNLSQPPTPSPPKPTGIFMTSANSSFEPVRSHSIGLKASDVDQLKMGGDLREGHNVPPSASLPAASPGNLEQPPDNLESIFLGKRNSHGNITSNGNLFHSAGDPPLDNISLLLERRPSSRAHGSNKKCESPATTLWAQGELPNFGGNVLLAPAAPSLYVPPKPQTEIIQPPEDGSYDSCSGGTDMGKFQTSLEEQISSENLENPPKITDEEQLPSQASSGYASLLSSPPTEVLQNQPLLIAQPNKTYNLSQPNNFSLSLPNKVKNINSLKELGAADKPGPQSLPPAVGNFNSNDNTVFSLIQVPPEASNPPNYKSAATNDKFSESLHNLSSILQRQSSANQMPLNLAAESQKALKGENATVDLANKNPIPAALPTGPRFPANPNGKPNYHAEKTVALDFTVPRAPVSSVANNVASSNSQPVAGGPLFPRPSVSYQNGQAGLDVNKQGFYQQVTKDGPPQQQPVMDIQHQGGVGLGEYMVPSSQPLSLVLPAQAPPVGKSVPYPPQAESQVQTLQNPSGTPYNSAPEPAPKYGPTEQPSYPAPPVPSSSATVNHIATYQEQPRPPASHTPQQADPYYYRSYGAYPQPYQPLDPRAAPLYYPDPYGAYDPTYRQYSGSAYVNPGNYSYQEPERPSSRSSHCSDRPSSRQGYSSKGDWNAHSGYYSGYYQSQYDYADPSRWDRYPGAYDPGYRDPSSNYWNYMYNSRDDVYQRNVPTAYTTRYDVYEDRWQYDPRYVGGFDEEPQRDLTKDDFDRRSVHSEHSVDSMHSGRSAHSRRSSFSSHSQHSQIYKSQPDLSANAYEPQIQNPPLNEYSYGVYPNDYGTQQALDDYSYGYPKGTEWQPVEQVPSRPLTPEKFGSPHICARFGSGGQLIKVLPNLPSDGQPALVEIHNMEVIMQNSAEQEQLRSFPGPLVKDETHKVDVISFAQKKFKECSQNESLLDQESACLLWDFIVLLCRQNGTVVGTDIAELLLRDHKTVWLPAKSPNEANLIDFSNEPLEQQEEESGASQLSFLTDSLQSTGEALEKETERFRELLLFGRKKDALESAMKNGLWGHALLLASKMDSRTHARVMTRFANSLPINDPLQTVYQLLSGRMPAAATCCGDEKWGDWRPHLAMVLSNLTNNVDVPTRAMTTMGDTLASRGLMDASHFCYLMAQVGFGIFTKKSTKLVLIGSNHSLSFAKFCTNEAIQRTEAYEYAQALGTQSFSLPNIQVYKFIYACRLAEHGLSAQAFHYCEVISKTILKHPSCYSPVLVSQLLEVSSHLRFFDPQLKEKPEQELFIDPPWLQHLRTLDKQMKHGTVVYDMGRTTPQQFACSTPSSEIDHVSQSDGFGIAGDIPTHTENPLLANLLPNMMPPVQAVLVPAGPENPPPVYPPYTGNDPSYPGTPQGLAPSSGPPTPSTAPNFVPFGVEQATVYPPAALQPYPPLPQATDCAPPMQMNQDPGLQRIQQNSPTKTTFADPEDFYGVMAKMVPGQRSRTVSQSSSHMRRTRTTSESSTHSIGSGRRSSMGIQPSPPPASIPEADRPEEKNKHKSHGPSQGSGKSWFPLKFGWLMGKGKNEAHLPDDRNKSIVWDEKQQRWTNLDEPEEERKPLPPPPPGMLKKPQTAPSVPGGPLNPSVNVFSIRAAGARGRYIDVLNPGGTRPTNSVPPPADLFAPLAPMPIPTNLFVPNAVPEEGQPAEASGAEMVQPAGQTGLSNFSQAQLPGLPPVSDLPTNQEDGQMGELSRSSSLSSLSREVSQHMEQAHVAPASGPPPTGAVPFYNPSQFSQQQPPSAQAGGLRPGRLGQRKYPSLK

Target ORF information:

RefSeq Version	XM_018232579.1
Organism	Xenopus laevis(African clawed frog)
Definition	Xenopus laevis protein transport protein Sec16A (LOC100037049), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_018232579.1

ORF Insert Sequence:

ATGCAGCCCC CTCCGCAAGC AAGGCATCCT GGCACGGGTG GACCTCCTCG CATGGGACTT 
GGCAATAATG CCCACTGGCG CAAAAGGGGA CCTAATCCTG TACCAGTAGC TTCAGCTTCA 
GTGCAGCCAG TAACAGACCC TTTTGCCTTT GGCCGTATTA CCCCACAAAG TATGGCACAG 
GGCAATGTCC CGATAGGAAA CCCACTGATG GCACCCAGTG TTTCCCCTTC TTTTTACCCT 
CAACCCGTTC CTGTACATGC ATCGGCTGCT GTAGATCAAG GGCATGGGTC TCCTGCATCA 
CTACCAGGAT TTGTTCCACA ACCTGCTGCT ACGGGGGGGA TGTATACAAA TGTGTCTGTC 
CCATCACCAT CAAGTGCCCC CTTTCACAAC TACCCAGCCG CCACCTCTAG TGCTCAGCCT 
GGACTCAGTA ATATTCAGAC TTTTCAGAAT ACAGCAAGTG GACTTGAAAA TGCCTTTCTG 
CCAGTGCACA TCAGGGCTCA GTCAACACAA GGCATTAATT ATAACCCTCC ATCTTCATCA 
CCTTTGCAGC ACTTTATCCC TGCACCATCT CAGCCAACTA ATGCTCCCTC TGGGTCCTTA 
CAAAGTGTAC CTCCTGCCTC AGCTCAGAAC TTCCCCCTTC CTGTTCAACA GCCATCCCAT 
AATGCTCCTT ATTACTCCCC ACCTTATTTA GAAGTGTCTA ATCTGGTCCC TCAAGCCAAC 
GAAACAGTGG CACAGCAGAG GTCAGCTCCT GGGCATTACC CGCCTGCCAC TGTCGCCAAT 
CCTACATTTC AACACAGCAC TTCACAACCC ACCATTTTAA ATCCGATAGA CTATATGCAC 
CGCCACAATC CTGACCAGAA CTGGGTTAAG CAACCTCACA AGGATCAGCA GCATCCACCT 
TTTCCCAGTC CTAACCCCGT ACAATACCCA ACTCAGCCAC CTGAGCCCCA TCAGGAGGTG 
ACTAATGCAT CTGGTCATGT CTCCACCAAT GTCGACTCTG GTACTATATC TATGTTTTTT 
AAAGGGGATG AAGAAGAAAA TGTAGAAATA GTTTCTTCCG ACCAGCAGAA TGCATCTGTT 
AAAGTGGATC ATGGTTCTTC TCATTACCAG AACTCTGGAC ATGAAGGTGT GCCTTCTCTC 
CTGCCACAGC CCACAGTCAG CAGCATGTCG TCACTTACAC CAATCTCTGT GTCTCAGAAT 
GTTGGCAATT TGACCCAACC ACATTCAGAT TGGAGGCAGG ATGTGTTTCC TGTTGGCGAT 
AAAATTTCTA AACCCAAAGT TGGAAGTGAA TTGCCCTATG ACAATGTTGA AAACATAGAG 
TGTGTACAAA ACCAAGAGGT TCCCCCCAAC GAGCCCAATC ATTTTAACCT TTATCCTTCT 
AATGTAAATT CAGATACGTA CCGCTATGGG TCCATGCCTG GACTCTCCCT ACAAAAGAAT 
GTTAGCAGCC AACCTGAAGC TGGTCCGAAT CTAGAGACGC CTGTCACAGT GGGTTGTCCT 
GTCAGATCTG ATAGTGTTTC ATCAAATTAT AGCAACCTGA GTCACAAAAG CCTGCCTGTG 
CCCACCCGGC CTCAAGAGCT AGCCGGGACA TTTATTCAGA AGGAGAGAGG AAGGCTTGAA 
GGTGACTCCT CCCATGGTTT CTTTAAGCAA ATTGATTCTT TGCCCATGGC AGGTGATGCT 
GTAGGCCAGA ATATGGGTCA GCACACGTTC CACAGCAATT TATCCCAGCC ACCAACTCCA 
AGTCCTCCTA AACCTACTGG GATCTTCATG ACGAGTGCAA ATAGTTCTTT CGAACCTGTT 
AGATCGCACA GCATTGGTTT GAAAGCGTCC GACGTAGACC AATTGAAAAT GGGTGGAGAC 
TTAAGAGAGG GGCATAATGT TCCACCAAGC GCCTCTCTCC CAGCAGCTTC CCCTGGTAAC 
TTGGAACAAC CACCAGATAA CCTGGAATCC ATATTCTTAG GTAAAAGAAA TTCTCACGGA 
AACATTACCA GCAATGGGAA CTTGTTCCAT TCTGCTGGCG ACCCGCCATT GGATAATATA 
TCCTTACTGC TTGAGAGACG TCCATCGTCA AGAGCACACG GGTCTAACAA GAAATGCGAA 
AGCCCGGCAA CCACCCTGTG GGCTCAAGGT GAGCTTCCAA ATTTTGGCGG AAATGTTTTG 
TTGGCTCCTG CAGCTCCTTC ACTCTATGTG CCCCCTAAAC CCCAAACCGA AATAATTCAG 
CCACCTGAAG ATGGGTCTTA TGATTCATGC TCGGGTGGAA CAGATATGGG GAAATTTCAG 
ACCTCTCTGG AAGAACAAAT TTCTTCGGAA AACCTTGAGA ATCCTCCAAA GATCACTGAT 
GAGGAGCAAC TTCCCTCGCA GGCAAGTTCA GGTTACGCCA GTTTGTTGTC TTCGCCGCCC 
ACTGAGGTAC TTCAGAATCA GCCCTTGCTA ATTGCCCAAC CAAATAAAAC CTATAATTTG 
TCACAGCCTA ATAATTTTTC ATTGTCGTTA CCAAATAAGG TAAAAAATAT TAATTCGCTT 
AAGGAACTGG GAGCAGCTGA CAAGCCGGGT CCTCAGAGTT TGCCTCCAGC TGTGGGGAAT 
TTTAACTCCA ATGACAATAC TGTGTTTTCC CTAATTCAAG TCCCACCCGA GGCTTCTAAT 
CCACCTAATT ACAAATCTGC TGCTACTAAT GATAAATTCT CTGAATCTCT TCACAATCTG 
AGTTCCATTT TGCAGCGGCA ATCATCAGCA AATCAAATGC CCCTCAATTT AGCTGCGGAA 
AGTCAAAAGG CACTTAAGGG TGAAAACGCG ACTGTCGATC TTGCAAATAA AAACCCGATT 
CCAGCTGCCC TTCCGACGGG GCCACGTTTC CCTGCCAATC CAAACGGCAA GCCAAATTAT 
CATGCAGAGA AGACCGTCGC TCTAGATTTT ACTGTCCCTC GTGCGCCAGT CAGTTCTGTT 
GCCAATAACG TTGCTTCAAG TAACAGTCAG CCGGTTGCTG GAGGGCCGCT CTTTCCTAGG 
CCGTCTGTCA GCTATCAGAA TGGTCAGGCA GGCCTAGATG TTAACAAGCA GGGTTTTTAT 
CAGCAGGTCA CTAAGGATGG GCCCCCGCAG CAGCAGCCTG TTATGGATAT CCAACACCAG 
GGAGGAGTGG GCCTGGGGGA ATACATGGTC CCATCCTCTC AGCCACTGTC CTTGGTTCTG 
CCTGCACAAG CGCCTCCAGT GGGTAAATCG GTTCCATACC CTCCACAAGC TGAAAGCCAA 
GTTCAAACCT TGCAGAACCC TAGTGGAACT CCTTATAACA GTGCGCCAGA ACCTGCACCT 
AAATACGGAC CAACTGAACA GCCTTCATAT CCAGCTCCGC CTGTTCCTTC CTCATCAGCC 
ACAGTGAACC ACATAGCAAC ATATCAAGAG CAGCCCAGGC CTCCTGCCTC CCATACCCCT 
CAGCAAGCTG ATCCTTATTA CTACAGGTCT TACGGTGCCT ACCCACAGCC TTACCAACCC 
TTGGACCCAA GGGCAGCACC TCTTTACTAT CCGGACCCTT ATGGCGCTTA TGACCCCACC 
TACAGGCAGT ACAGTGGGTC TGCATATGTG AACCCTGGCA ATTACTCCTA TCAGGAGCCC 
GAACGTCCCA GTTCCAGATC CAGCCACTGC TCCGATCGCC CCTCCTCAAG GCAGGGCTAT 
AGCAGTAAAG GAGACTGGAA TGCACACAGT GGTTACTACT CAGGGTATTA TCAGAGTCAG 
TATGATTATG CAGACCCCAG CCGCTGGGAC CGATATCCTG GAGCCTATGA CCCTGGCTAT 
AGAGACCCCA GCAGTAATTA CTGGAATTAT ATGTACAACA GCCGTGATGA TGTGTACCAA 
AGGAACGTGC CTACTGCTTA TACCACCAGA TATGACGTTT ATGAAGACCG CTGGCAATAT 
GACCCACGTT ATGTGGGAGG CTTTGATGAA GAGCCACAGA GAGACCTCAC GAAGGACGAC 
TTTGACCGAC GTAGTGTTCA CAGTGAGCAT TCGGTCGATA GCATGCACAG CGGCCGGAGT 
GCACACAGTC GGAGAAGCAG TTTCAGCTCC CATTCACAAC ATAGCCAGAT CTACAAAAGC 
CAGCCAGACC TGTCTGCCAA CGCGTATGAA CCCCAAATAC AGAATCCTCC TCTCAATGAG 
TATTCGTATG GCGTGTACCC CAATGACTAC GGCACCCAGC AGGCGCTTGA TGATTACTCT 
TACGGGTATC CCAAGGGCAC AGAGTGGCAA CCTGTAGAAC AAGTGCCTTC AAGGCCGTTG 
ACTCCAGAGA AGTTTGGGAG CCCACACATC TGTGCCAGGT TTGGTTCTGG GGGTCAGTTA 
ATAAAGGTGC TGCCTAACCT TCCTTCAGAC GGACAGCCGG CCTTAGTGGA AATCCACAAC 
ATGGAGGTCA TTATGCAGAA CAGCGCAGAG CAGGAACAGC TGAGAAGTTT CCCTGGACCT 
TTAGTGAAGG ATGAAACTCA TAAAGTTGAC GTCATCAGTT TTGCCCAGAA GAAATTCAAG 
GAGTGTTCTC AGAATGAGAG CCTGTTAGAC CAAGAGTCTG CTTGCCTGCT CTGGGACTTC 
ATTGTGCTTC TGTGCCGGCA AAATGGGACT GTTGTTGGAA CAGATATCGC TGAGCTGCTT 
TTGAGAGATC ACAAGACAGT GTGGCTTCCT GCAAAGTCTC CCAACGAAGC CAACCTGATC 
GATTTCAGCA ATGAGCCGCT CGAGCAGCAG GAGGAAGAGT CAGGGGCTTC TCAGTTGTCC 
TTCCTCACGG ATTCTTTGCA AAGCACCGGG GAAGCTCTAG AGAAGGAGAC TGAGCGGTTC 
AGAGAGCTTC TACTTTTTGG GCGTAAAAAG GATGCCTTAG AATCTGCTAT GAAAAATGGA 
CTGTGGGGCC ACGCTTTGCT CCTAGCTAGC AAAATGGACA GCCGAACGCA CGCCAGAGTT 
ATGACAAGGT TTGCCAACAG CCTTCCAATT AACGATCCCC TGCAGACTGT GTATCAGCTC 
TTGTCTGGGA GAATGCCAGC AGCAGCCACG TGCTGCGGAG ATGAGAAATG GGGTGACTGG 
CGCCCTCACC TGGCGATGGT GCTGTCTAAT CTGACCAACA ATGTTGACGT TCCCACCAGG 
GCCATGACAA CAATGGGAGA TACATTAGCA TCCCGGGGGC TGATGGATGC CTCACACTTC 
TGCTATTTAA TGGCACAAGT CGGCTTTGGC ATTTTCACCA AGAAATCTAC TAAGTTGGTC 
CTCATTGGAT CCAACCACAG TTTATCATTT GCAAAGTTTT GCACTAACGA AGCCATCCAG 
AGAACAGAGG CTTATGAATA TGCACAGGCA CTTGGCACCC AGTCTTTCTC TCTCCCGAAT 
ATACAGGTTT ACAAGTTCAT CTACGCTTGC CGTTTGGCGG AACACGGATT ATCTGCACAA 
GCTTTCCATT ACTGTGAAGT CATCTCCAAG ACCATCTTGA AGCACCCGTC TTGCTACTCC 
CCTGTGCTTG TCAGCCAGCT ACTGGAGGTG TCCTCCCACT TGAGGTTCTT TGACCCACAG 
CTGAAGGAGA AGCCAGAACA GGAGTTATTT ATCGACCCTC CTTGGCTGCA GCATCTCCGC 
ACCTTGGATA AGCAAATGAA GCATGGTACT GTAGTTTATG ACATGGGCAG AACCACACCT 
CAGCAGTTTG CCTGCAGTAC ACCAAGCTCG GAGATAGACC ATGTGAGCCA ATCAGATGGT 
TTTGGAATAG CCGGGGATAT ACCAACTCAT ACAGAGAATC CATTACTGGC CAATCTCCTG 
CCCAACATGA TGCCTCCAGT ACAAGCTGTG CTAGTTCCTG CTGGTCCTGA AAATCCGCCA 
CCTGTATATC CACCCTACAC AGGGAATGAT CCCTCCTACC CAGGTACACC TCAGGGTCTA 
GCTCCCTCAT CTGGGCCTCC AACTCCATCT ACTGCTCCAA ATTTTGTGCC GTTTGGTGTG 
GAACAGGCAA CTGTTTATCC ACCTGCTGCT CTACAGCCAT ACCCACCTCT TCCCCAGGCA 
ACAGATTGTG CTCCTCCAAT GCAGATGAAT CAAGATCCAG GACTGCAAAG GATTCAGCAG 
AATTCCCCTA CCAAAACCAC CTTCGCTGAT CCTGAAGATT TCTACGGTGT CATGGCAAAA 
ATGGTTCCCG GGCAGAGATC ACGGACAGTG TCACAGTCCT CATCACACAT GAGGCGGACA 
CGGACAACCT CAGAGTCATC AACCCATTCT ATTGGGTCTG GACGTAGAAG TTCCATGGGC 
ATCCAACCAT CGCCTCCTCC TGCCTCTATC CCAGAGGCAG ACAGACCAGA AGAGAAGAAC 
AAACATAAGT CTCATGGTCC ATCCCAAGGG AGTGGTAAAA GTTGGTTTCC CCTGAAATTT 
GGCTGGTTGA TGGGAAAAGG CAAGAACGAA GCACACTTAC CTGATGACAG GAATAAATCT 
ATTGTGTGGG ATGAGAAGCA GCAGCGGTGG ACTAACCTGG ATGAACCAGA AGAAGAGAGA 
AAACCGTTGC CTCCACCACC ACCAGGGATG CTGAAAAAAC CACAGACTGC TCCATCTGTC 
CCAGGTGGTC CTCTCAACCC TTCTGTCAAT GTTTTCTCCA TCAGAGCAGC AGGGGCAAGA 
GGTCGTTACA TTGATGTCTT AAACCCAGGG GGTACCCGGC CCACTAACTC GGTCCCTCCG 
CCTGCTGATT TGTTTGCACC TTTGGCCCCC ATGCCTATCC CCACGAACCT GTTTGTTCCA 
AATGCAGTCC CAGAAGAGGG TCAGCCTGCG GAAGCCAGTG GGGCAGAAAT GGTGCAACCT 
GCGGGTCAGA CGGGGCTCAG TAACTTTTCC CAGGCACAGT TACCCGGTTT GCCCCCTGTG 
TCTGATCTTC CAACAAATCA GGAAGATGGG CAGATGGGAG AGCTTTCCCG ATCTAGCTCT 
CTCAGTTCCT TATCCCGTGA AGTAAGCCAG CATATGGAGC AGGCTCACGT GGCCCCTGCG 
TCAGGCCCCC CACCTACAGG GGCTGTCCCC TTCTACAACC CCTCCCAGTT TTCACAACAG 
CAGCCTCCTT CTGCCCAAGC AGGTGGGCTA CGACCTGGAC GTCTCGGCCA GAGGAAATAT 
CCTTCCTTAA AATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC100037049 ( XM_018232580.1 ) cDNA ORF clone, Xenopus laevis(African clawed frog) -> XP_018088069.1 Xenopus laevis protein transport protein Sec16A (LOC100037049), transcript variant X2, mRNA.

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CloneID	OAf43840
Clone ID Related Accession (Same CDS sequence)	XM_018232580.1
Accession Version	XM_018232580.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7059bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1474214400000
Organism	Xenopus laevis(African clawed frog)
Product	protein transport protein Sec16A isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030739.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus laevis Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAGCCCC CTCCGCAAGC AAGGCATCCT GGCACGGGTG GACCTCCTCG CATGGGACTT 
GGCAATAATG CCCACTGGCG CAAAAGGGGA CCTAATCCTG TACCAGTAGC TTCAGCTTCA 
GTGCAGCCAG TAACAGACCC TTTTGCCTTT GGCCGTATTA CCCCACAAAG TATGGCACAG 
GGCAATGTCC CGATAGGAAA CCCACTGATG GCACCCAGTG TTTCCCCTTC TTTTTACCCT 
CAACCCGTTC CTGTACATGC ATCGGCTGCT GTAGATCAAG GGCATGGGTC TCCTGCATCA 
CTACCAGGAT TTGTTCCACA ACCTGCTGCT ACGGGGGGGA TGTATACAAA TGTGTCTGTC 
CCATCACCAT CAAGTGCCCC CTTTCACAAC TACCCAGCCG CCACCTCTAG TGCTCAGCCT 
GGACTCAGTA ATATTCAGAC TTTTCAGAAT ACAGCAAGTG GACTTGAAAA TGCCTTTCTG 
CCAGTGCACA TCAGGGCTCA GTCAACACAA GGCATTAATT ATAACCCTCC ATCTTCATCA 
CCTTTGCAGC ACTTTATCCC TGCACCATCT CAGCCAACTA ATGCTCCCTC TGGGTCCTTA 
CAAAGTGTAC CTCCTGCCTC AGCTCAGAAC TTCCCCCTTC CTGTTCAACA GCCATCCCAT 
AATGCTCCTT ATTACTCCCC ACCTTATTTA GAAGTGTCTA ATCTGGTCCC TCAAGCCAAC 
GAAACAGTGG CACAGCAGAG GTCAGCTCCT GGGCATTACC CGCCTGCCAC TGTCGCCAAT 
CCTACATTTC AACACAGCAC TTCACAACCC ACCATTTTAA ATCCGATAGA CTATATGCAC 
CGCCACAATC CTGACCAGAA CTGGGTTAAG CAACCTCACA AGGATCAGCA GCATCCACCT 
TTTCCCAGTC CTAACCCCGT ACAATACCCA ACTCAGCCAC CTGAGCCCCA TCAGGAGGTG 
ACTAATGCAT CTGGTCATGT CTCCACCAAT GTCGACTCTG GTACTATATC TATGTTTTTT 
AAAGGGGATG AAGAAGAAAA TGTAGAAATA GTTTCTTCCG ACCAGCAGAA TGCATCTGTT 
AAAGTGGATC ATGGTTCTTC TCATTACCAG AACTCTGGAC ATGAAGGTGT GCCTTCTCTC 
CTGCCACAGC CCACAGTCAG CAGCATGTCG TCACTTACAC CAATCTCTGT GTCTCAGAAT 
GTTGGCAATT TGACCCAACC ACATTCAGAT TGGAGGCAGG ATGTGTTTCC TGTTGGCGAT 
AAAATTTCTA AACCCAAAGT TGGAAGTGAA TTGCCCTATG ACAATGTTGA AAACATAGAG 
TGTGTACAAA ACCAAGAGGT TCCCCCCAAC GAGCCCAATC ATTTTAACCT TTATCCTTCT 
AATGTAAATT CAGATACGTA CCGCTATGGG TCCATGCCTG GACTCTCCCT ACAAAAGAAT 
GTTAGCAGCC AACCTGAAGC TGGTCCGAAT CTAGAGACGC CTGTCACAGT GGGTTGTCCT 
GTCAGATCTG ATAGTGTTTC ATCAAATTAT AGCAACCTGA GTCACAAAAG CCTGCCTGTG 
CCCACCCGGC CTCAAGAGCT AGCCGGGACA TTTATTCAGA AGGAGAGAGG AAGGCTTGAA 
GGTGACTCCT CCCATGGTTT CTTTAAGCAA ATTGATTCTT TGCCCATGGC AGGTGATGCT 
GTAGGCCAGA ATATGGGTCA GCACACGTTC CACAGCAATT TATCCCAGCC ACCAACTCCA 
AGTCCTCCTA AACCTACTGG GATCTTCATG ACGAGTGCAA ATAGTTCTTT CGAACCTGTT 
AGATCGCACA GCATTGGTTT GAAAGCGTCC GACGTAGACC AATTGAAAAT GGGTGGAGAC 
TTAAGAGAGG GGCATAATGT TCCACCAAGC GCCTCTCTCC CAGCAGCTTC CCCTGGTAAC 
TTGGAACAAC CACCAGATAA CCTGGAATCC ATATTCTTAG GTAAAAGAAA TTCTCACGGA 
AACATTACCA GCAATGGGAA CTTGTTCCAT TCTGCTGGCG ACCCGCCATT GGATAATATA 
TCCTTACTGC TTGAGAGACG TCCATCGTCA AGAGCACACG GGTCTAACAA GAAATGCGAA 
AGCCCGGCAA CCACCCTGTG GGCTCAAGGT GAGCTTCCAA ATTTTGGCGG AAATGTTTTG 
TTGGCTCCTG CAGCTCCTTC ACTCTATGTG CCCCCTAAAC CCCAAACCGA AATAATTCAG 
CCACCTGAAG ATGGGTCTTA TGATTCATGC TCGGGTGGAA CAGATATGGG GAAATTTCAG 
ACCTCTCTGG AAGAACAAAT TTCTTCGGAA AACCTTGAGA ATCCTCCAAA GATCACTGAT 
GAGGAGCAAC TTCCCTCGCA GGCAAGTTCA GGTTACGCCA GTTTGTTGTC TTCGCCGCCC 
ACTGAGGTAC TTCAGAATCA GCCCTTGCTA ATTGCCCAAC CAAATAAAAC CTATAATTTG 
TCACAGCCTA ATAATTTTTC ATTGTCGTTA CCAAATAAGG TAAAAAATAT TAATTCGCTT 
AAGGAACTGG GAGCAGCTGA CAAGCCGGGT CCTCAGAGTT TGCCTCCAGC TGTGGGGAAT 
TTTAACTCCA ATGACAATAC TGTGTTTTCC CTAATTCAAG TCCCACCCGA GGCTTCTAAT 
CCACCTAATT ACAAATCTGC TGCTACTAAT GATAAATTCT CTGAATCTCT TCACAATCTG 
AGTTCCATTT TGCAGCGGCA ATCATCAGCA AATCAAATGC CCCTCAATTT AGCTGCGGAA 
AGTCAAAAGG CACTTAAGGG TGAAAACGCG ACTGTCGATC TTGCAAATAA AAACCCGATT 
CCAGCTGCCC TTCCGACGGG GCCACGTTTC CCTGCCAATC CAAACGGCAA GCCAAATTAT 
CATGCAGAGA AGACCGTCGC TCTAGATTTT ACTGTCCCTC GTGCGCCAGT CAGTTCTGTT 
GCCAATAACG TTGCTTCAAG TAACAGTCAG CCGGTTGCTG GAGGGCCGCT CTTTCCTAGG 
CCGTCTGTCA GCTATCAGAA TGGTCAGGCA GGCCTAGATG TTAACAAGCA GGGTTTTTAT 
CAGCAGGTCA CTAAGGATGG GCCCCCGCAG CAGCAGCCTG TTATGGATAT CCAACACCAG 
GGAGGAGTGG GCCTGGGGGA ATACATGGTC CCATCCTCTC AGCCACTGTC CTTGGTTCTG 
CCTGCACAAG CGCCTCCAGT GGGTAAATCG GTTCCATACC CTCCACAAGC TGAAAGCCAA 
GTTCAAACCT TGCAGAACCC TAGTGGAACT CCTTATAACA GTGCGCCAGA ACCTGCACCT 
AAATACGGAC CAACTGAACA GCCTTCATAT CCAGCTCCGC CTGTTCCTTC CTCATCAGCC 
ACAGTGAACC ACATAGCAAC ATATCAAGAG CAGCCCAGGC CTCCTGCCTC CCATACCCCT 
CAGCAAGCTG ATCCTTATTA CTACAGGTCT TACGGTGCCT ACCCACAGCC TTACCAACCC 
TTGGACCCAA GGGCAGCACC TCTTTACTAT CCGGACCCTT ATGGCGCTTA TGACCCCACC 
TACAGGCAGT ACAGTGGGTC TGCATATGTG AACCCTGGCA ATTACTCCTA TCAGGAGCCC 
GAACGTCCCA GTTCCAGATC CAGCCACTGC TCCGATCGCC CCTCCTCAAG GCAGGGCTAT 
AGCAGTAAAG GAGACTGGAA TGCACACAGT GGTTACTACT CAGGGTATTA TCAGAGTCAG 
TATGATTATG CAGACCCCAG CCGCTGGGAC CGATATCCTG GAGCCTATGA CCCTGGCTAT 
AGAGACCCCA GCAGTAATTA CTGGAATTAT ATGTACAACA GCCGTGATGA TGTGTACCAA 
AGGAACGTGC CTACTGCTTA TACCACCAGA TATGACGTTT ATGAAGACCG CTGGCAATAT 
GACCCACGTT ATGTGGGAGG CTTTGATGAA GAGCCACAGA GAGACCTCAC GAAGGACGAC 
TTTGACCGAC GTAGTGTTCA CAGTGAGCAT TCGGTCGATA GCATGCACAG CGGCCGGAGT 
GCACACAGTC GGAGAAGCAG TTTCAGCTCC CATTCACAAC ATAGCCAGAT CTACAAAAGC 
CAGCCAGACC TGTCTGCCAA CGCGTATGAA CCCCAAATAC AGAATCCTCC TCTCAATGAG 
TATTCGTATG GCGTGTACCC CAATGACTAC GGCACCCAGC AGGCGCTTGA TGATTACTCT 
TACGGGTATC CCAAGGGCAC AGAGTGGCAA CCTGTAGAAC AAGTGCCTTC AAGGCCGTTG 
ACTCCAGAGA AGTTTGGGAG CCCACACATC TGTGCCAGGT TTGGTTCTGG GGGTCAGTTA 
ATAAAGGTGC TGCCTAACCT TCCTTCAGAC GGACAGCCGG CCTTAGTGGA AATCCACAAC 
ATGGAGGTCA TTATGCAGAA CAGCGCAGAG CAGGAACAGC TGAGAAGTTT CCCTGGACCT 
TTAGTGAAGG ATGAAACTCA TAAAGTTGAC GTCATCAGTT TTGCCCAGAA GAAATTCAAG 
GAGTGTTCTC AGAATGAGAG CCTGTTAGAC CAAGAGTCTG CTTGCCTGCT CTGGGACTTC 
ATTGTGCTTC TGTGCCGGCA AAATGGGACT GTTGTTGGAA CAGATATCGC TGAGCTGCTT 
TTGAGAGATC ACAAGACAGT GTGGCTTCCT GCAAAGTCTC CCAACGAAGC CAACCTGATC 
GATTTCAGCA ATGAGCCGCT CGAGCAGCAG GAGGAAGAGT CAGGGGCTTC TCAGTTGTCC 
TTCCTCACGG ATTCTTTGCA AAGCACCGGG GAAGCTCTAG AGAAGGAGAC TGAGCGGTTC 
AGAGAGCTTC TACTTTTTGG GCGTAAAAAG GATGCCTTAG AATCTGCTAT GAAAAATGGA 
CTGTGGGGCC ACGCTTTGCT CCTAGCTAGC AAAATGGACA GCCGAACGCA CGCCAGAGTT 
ATGACAAGGT TTGCCAACAG CCTTCCAATT AACGATCCCC TGCAGACTGT GTATCAGCTC 
TTGTCTGGGA GAATGCCAGC AGCAGCCACG TGCTGCGGAG ATGAGAAATG GGGTGACTGG 
CGCCCTCACC TGGCGATGGT GCTGTCTAAT CTGACCAACA ATGTTGACGT TCCCACCAGG 
GCCATGACAA CAATGGGAGA TACATTAGCA TCCCGGGGGC TGATGGATGC CTCACACTTC 
TGCTATTTAA TGGCACAAGT CGGCTTTGGC ATTTTCACCA AGAAATCTAC TAAGTTGGTC 
CTCATTGGAT CCAACCACAG TTTATCATTT GCAAAGTTTT GCACTAACGA AGCCATCCAG 
AGAACAGAGG CTTATGAATA TGCACAGGCA CTTGGCACCC AGTCTTTCTC TCTCCCGAAT 
ATACAGGTTT ACAAGTTCAT CTACGCTTGC CGTTTGGCGG AACACGGATT ATCTGCACAA 
GCTTTCCATT ACTGTGAAGT CATCTCCAAG ACCATCTTGA AGCACCCGTC TTGCTACTCC 
CCTGTGCTTG TCAGCCAGCT ACTGGAGGTG TCCTCCCACT TGAGGTTCTT TGACCCACAG 
CTGAAGGAGA AGCCAGAACA GGAGTTATTT ATCGACCCTC CTTGGCTGCA GCATCTCCGC 
ACCTTGGATA AGCAAATGAA GCATGGTACT GTAGTTTATG ACATGGGCAG AACCACACCT 
CAGCAGTTTG CCTGCAGTAC ACCAAGCTCG GAGATAGACC ATGTGAGCCA ATCAGATGGT 
TTTGGAATAG CCGGGGATAT ACCAACTCAT ACAGAGAATC CATTACTGGC CAATCTCCTG 
CCCAACATGA TGCCTCCAGT ACAAGCTGTG CTAGTTCCTG CTGGTCCTGA AAATCCGCCA 
CCTGTATATC CACCCTACAC AGGGAATGAT CCCTCCTACC CAGGTACACC TCAGGGTCTA 
GCTCCCTCAT CTGGGCCTCC AACTCCATCT ACTGCTCCAA ATTTTGTGCC GTTTGGTGTG 
GAACAGGCAA CTGTTTATCC ACCTGCTGCT CTACAGCCAT ACCCACCTCT TCCCCAGGCA 
ACAGATTGTG CTCCTCCAAT GCAGATGAAT CAAGATCCAG GACTGCAAAG GATTCAGCAG 
AATTCCCCTA CCAAAACCAC CTTCGCTGAT CCTGAAGATT TCTACGGTGT CATGGCAAAA 
ATGGTTCCCG GGCAGAGATC ACGGACAGTG TCACAGTCCT CATCACACAT GAGGCGGACA 
CGGACAACCT CAGAGTCATC AACCCATTCT ATTGGGTCTG GACGTAGAAG TTCCATGGGC 
ATCCAACCAT CGCCTCCTCC TGCCTCTATC CCAGAGGCAG ACAGACCAGA AGAGAAGAAC 
AAACATAAGT CTCATGGTCC ATCCCAAGGG AGTGGTAAAA GTTGGTTTCC CCTGAAATTT 
GGCTGGTTGA TGGGAAAAGG CAAGAACGAA GCACACTTAC CTGATGACAG GAATAAATCT 
ATTGTGTGGG ATGAGAAGCA GCAGCGGTGG ACTAACCTGG ATGAACCAGA AGAAGAGAGA 
AAACCGTTGC CTCCACCACC ACCAGGGATG CTGAAAAAAC CACAGACTGC TCCATCTGTC 
CCAGGTGGTC CTCTCAACCC TTCTGTCAAT GTTTTCTCCA TCAGAGCAGC AGGGGCAAGA 
GGTCGTTACA TTGATGTCTT AAACCCAGGG GGTACCCGGC CCACTAACTC GGTCCCTCCG 
CCTGCTGATT TGTTTGCACC TTTGGCCCCC ATGCCTATCC CCACGAACCT GTTTGTTCCA 
AATGCAGTCC CAGAAGAGGG TCAGCCTGCG GAAGCCAGTG GGGCAGAAAT GGTGCAACCT 
GCGGGTCAGA CGGGGCTCAG TAACTTTTCC CAGGCACAGT TACCCGGTTT GCCCCCTGTG 
TCTGATCTTC CAACAAATCA GGAAGATGGG CAGATGGGAG AGCTTTCCCG ATCTAGCTCT 
CTCAGTTCCT TATCCCGTGA AGTAAGCCAG CATATGGAGC AGCTCTCTGC CTCTCCCAGG 
AACTCTACTG CAACAGCAGC ACCTTCTGGC TCTCCAACTG TGGCTGAACT ACAGTTCAGG 
ATCACTGTTA GCTGCTCCCT TCTACTCTGC AGCTGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_018088069.1
CDS	215..7273
Translation	MQPPPQARHPGTGGPPRMGLGNNAHWRKRGPNPVPVASASVQPVTDPFAFGRITPQSMAQGNVPIGNPLMAPSVSPSFYPQPVPVHASAAVDQGHGSPASLPGFVPQPAATGGMYTNVSVPSPSSAPFHNYPAATSSAQPGLSNIQTFQNTASGLENAFLPVHIRAQSTQGINYNPPSSSPLQHFIPAPSQPTNAPSGSLQSVPPASAQNFPLPVQQPSHNAPYYSPPYLEVSNLVPQANETVAQQRSAPGHYPPATVANPTFQHSTSQPTILNPIDYMHRHNPDQNWVKQPHKDQQHPPFPSPNPVQYPTQPPEPHQEVTNASGHVSTNVDSGTISMFFKGDEEENVEIVSSDQQNASVKVDHGSSHYQNSGHEGVPSLLPQPTVSSMSSLTPISVSQNVGNLTQPHSDWRQDVFPVGDKISKPKVGSELPYDNVENIECVQNQEVPPNEPNHFNLYPSNVNSDTYRYGSMPGLSLQKNVSSQPEAGPNLETPVTVGCPVRSDSVSSNYSNLSHKSLPVPTRPQELAGTFIQKERGRLEGDSSHGFFKQIDSLPMAGDAVGQNMGQHTFHSNLSQPPTPSPPKPTGIFMTSANSSFEPVRSHSIGLKASDVDQLKMGGDLREGHNVPPSASLPAASPGNLEQPPDNLESIFLGKRNSHGNITSNGNLFHSAGDPPLDNISLLLERRPSSRAHGSNKKCESPATTLWAQGELPNFGGNVLLAPAAPSLYVPPKPQTEIIQPPEDGSYDSCSGGTDMGKFQTSLEEQISSENLENPPKITDEEQLPSQASSGYASLLSSPPTEVLQNQPLLIAQPNKTYNLSQPNNFSLSLPNKVKNINSLKELGAADKPGPQSLPPAVGNFNSNDNTVFSLIQVPPEASNPPNYKSAATNDKFSESLHNLSSILQRQSSANQMPLNLAAESQKALKGENATVDLANKNPIPAALPTGPRFPANPNGKPNYHAEKTVALDFTVPRAPVSSVANNVASSNSQPVAGGPLFPRPSVSYQNGQAGLDVNKQGFYQQVTKDGPPQQQPVMDIQHQGGVGLGEYMVPSSQPLSLVLPAQAPPVGKSVPYPPQAESQVQTLQNPSGTPYNSAPEPAPKYGPTEQPSYPAPPVPSSSATVNHIATYQEQPRPPASHTPQQADPYYYRSYGAYPQPYQPLDPRAAPLYYPDPYGAYDPTYRQYSGSAYVNPGNYSYQEPERPSSRSSHCSDRPSSRQGYSSKGDWNAHSGYYSGYYQSQYDYADPSRWDRYPGAYDPGYRDPSSNYWNYMYNSRDDVYQRNVPTAYTTRYDVYEDRWQYDPRYVGGFDEEPQRDLTKDDFDRRSVHSEHSVDSMHSGRSAHSRRSSFSSHSQHSQIYKSQPDLSANAYEPQIQNPPLNEYSYGVYPNDYGTQQALDDYSYGYPKGTEWQPVEQVPSRPLTPEKFGSPHICARFGSGGQLIKVLPNLPSDGQPALVEIHNMEVIMQNSAEQEQLRSFPGPLVKDETHKVDVISFAQKKFKECSQNESLLDQESACLLWDFIVLLCRQNGTVVGTDIAELLLRDHKTVWLPAKSPNEANLIDFSNEPLEQQEEESGASQLSFLTDSLQSTGEALEKETERFRELLLFGRKKDALESAMKNGLWGHALLLASKMDSRTHARVMTRFANSLPINDPLQTVYQLLSGRMPAAATCCGDEKWGDWRPHLAMVLSNLTNNVDVPTRAMTTMGDTLASRGLMDASHFCYLMAQVGFGIFTKKSTKLVLIGSNHSLSFAKFCTNEAIQRTEAYEYAQALGTQSFSLPNIQVYKFIYACRLAEHGLSAQAFHYCEVISKTILKHPSCYSPVLVSQLLEVSSHLRFFDPQLKEKPEQELFIDPPWLQHLRTLDKQMKHGTVVYDMGRTTPQQFACSTPSSEIDHVSQSDGFGIAGDIPTHTENPLLANLLPNMMPPVQAVLVPAGPENPPPVYPPYTGNDPSYPGTPQGLAPSSGPPTPSTAPNFVPFGVEQATVYPPAALQPYPPLPQATDCAPPMQMNQDPGLQRIQQNSPTKTTFADPEDFYGVMAKMVPGQRSRTVSQSSSHMRRTRTTSESSTHSIGSGRRSSMGIQPSPPPASIPEADRPEEKNKHKSHGPSQGSGKSWFPLKFGWLMGKGKNEAHLPDDRNKSIVWDEKQQRWTNLDEPEEERKPLPPPPPGMLKKPQTAPSVPGGPLNPSVNVFSIRAAGARGRYIDVLNPGGTRPTNSVPPPADLFAPLAPMPIPTNLFVPNAVPEEGQPAEASGAEMVQPAGQTGLSNFSQAQLPGLPPVSDLPTNQEDGQMGELSRSSSLSSLSREVSQHMEQLSASPRNSTATAAPSGSPTVAELQFRITVSCSLLLCSC

Target ORF information:

RefSeq Version	XM_018232580.1
Organism	Xenopus laevis(African clawed frog)
Definition	Xenopus laevis protein transport protein Sec16A (LOC100037049), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_018232580.1

ORF Insert Sequence:

ATGCAGCCCC CTCCGCAAGC AAGGCATCCT GGCACGGGTG GACCTCCTCG CATGGGACTT 
GGCAATAATG CCCACTGGCG CAAAAGGGGA CCTAATCCTG TACCAGTAGC TTCAGCTTCA 
GTGCAGCCAG TAACAGACCC TTTTGCCTTT GGCCGTATTA CCCCACAAAG TATGGCACAG 
GGCAATGTCC CGATAGGAAA CCCACTGATG GCACCCAGTG TTTCCCCTTC TTTTTACCCT 
CAACCCGTTC CTGTACATGC ATCGGCTGCT GTAGATCAAG GGCATGGGTC TCCTGCATCA 
CTACCAGGAT TTGTTCCACA ACCTGCTGCT ACGGGGGGGA TGTATACAAA TGTGTCTGTC 
CCATCACCAT CAAGTGCCCC CTTTCACAAC TACCCAGCCG CCACCTCTAG TGCTCAGCCT 
GGACTCAGTA ATATTCAGAC TTTTCAGAAT ACAGCAAGTG GACTTGAAAA TGCCTTTCTG 
CCAGTGCACA TCAGGGCTCA GTCAACACAA GGCATTAATT ATAACCCTCC ATCTTCATCA 
CCTTTGCAGC ACTTTATCCC TGCACCATCT CAGCCAACTA ATGCTCCCTC TGGGTCCTTA 
CAAAGTGTAC CTCCTGCCTC AGCTCAGAAC TTCCCCCTTC CTGTTCAACA GCCATCCCAT 
AATGCTCCTT ATTACTCCCC ACCTTATTTA GAAGTGTCTA ATCTGGTCCC TCAAGCCAAC 
GAAACAGTGG CACAGCAGAG GTCAGCTCCT GGGCATTACC CGCCTGCCAC TGTCGCCAAT 
CCTACATTTC AACACAGCAC TTCACAACCC ACCATTTTAA ATCCGATAGA CTATATGCAC 
CGCCACAATC CTGACCAGAA CTGGGTTAAG CAACCTCACA AGGATCAGCA GCATCCACCT 
TTTCCCAGTC CTAACCCCGT ACAATACCCA ACTCAGCCAC CTGAGCCCCA TCAGGAGGTG 
ACTAATGCAT CTGGTCATGT CTCCACCAAT GTCGACTCTG GTACTATATC TATGTTTTTT 
AAAGGGGATG AAGAAGAAAA TGTAGAAATA GTTTCTTCCG ACCAGCAGAA TGCATCTGTT 
AAAGTGGATC ATGGTTCTTC TCATTACCAG AACTCTGGAC ATGAAGGTGT GCCTTCTCTC 
CTGCCACAGC CCACAGTCAG CAGCATGTCG TCACTTACAC CAATCTCTGT GTCTCAGAAT 
GTTGGCAATT TGACCCAACC ACATTCAGAT TGGAGGCAGG ATGTGTTTCC TGTTGGCGAT 
AAAATTTCTA AACCCAAAGT TGGAAGTGAA TTGCCCTATG ACAATGTTGA AAACATAGAG 
TGTGTACAAA ACCAAGAGGT TCCCCCCAAC GAGCCCAATC ATTTTAACCT TTATCCTTCT 
AATGTAAATT CAGATACGTA CCGCTATGGG TCCATGCCTG GACTCTCCCT ACAAAAGAAT 
GTTAGCAGCC AACCTGAAGC TGGTCCGAAT CTAGAGACGC CTGTCACAGT GGGTTGTCCT 
GTCAGATCTG ATAGTGTTTC ATCAAATTAT AGCAACCTGA GTCACAAAAG CCTGCCTGTG 
CCCACCCGGC CTCAAGAGCT AGCCGGGACA TTTATTCAGA AGGAGAGAGG AAGGCTTGAA 
GGTGACTCCT CCCATGGTTT CTTTAAGCAA ATTGATTCTT TGCCCATGGC AGGTGATGCT 
GTAGGCCAGA ATATGGGTCA GCACACGTTC CACAGCAATT TATCCCAGCC ACCAACTCCA 
AGTCCTCCTA AACCTACTGG GATCTTCATG ACGAGTGCAA ATAGTTCTTT CGAACCTGTT 
AGATCGCACA GCATTGGTTT GAAAGCGTCC GACGTAGACC AATTGAAAAT GGGTGGAGAC 
TTAAGAGAGG GGCATAATGT TCCACCAAGC GCCTCTCTCC CAGCAGCTTC CCCTGGTAAC 
TTGGAACAAC CACCAGATAA CCTGGAATCC ATATTCTTAG GTAAAAGAAA TTCTCACGGA 
AACATTACCA GCAATGGGAA CTTGTTCCAT TCTGCTGGCG ACCCGCCATT GGATAATATA 
TCCTTACTGC TTGAGAGACG TCCATCGTCA AGAGCACACG GGTCTAACAA GAAATGCGAA 
AGCCCGGCAA CCACCCTGTG GGCTCAAGGT GAGCTTCCAA ATTTTGGCGG AAATGTTTTG 
TTGGCTCCTG CAGCTCCTTC ACTCTATGTG CCCCCTAAAC CCCAAACCGA AATAATTCAG 
CCACCTGAAG ATGGGTCTTA TGATTCATGC TCGGGTGGAA CAGATATGGG GAAATTTCAG 
ACCTCTCTGG AAGAACAAAT TTCTTCGGAA AACCTTGAGA ATCCTCCAAA GATCACTGAT 
GAGGAGCAAC TTCCCTCGCA GGCAAGTTCA GGTTACGCCA GTTTGTTGTC TTCGCCGCCC 
ACTGAGGTAC TTCAGAATCA GCCCTTGCTA ATTGCCCAAC CAAATAAAAC CTATAATTTG 
TCACAGCCTA ATAATTTTTC ATTGTCGTTA CCAAATAAGG TAAAAAATAT TAATTCGCTT 
AAGGAACTGG GAGCAGCTGA CAAGCCGGGT CCTCAGAGTT TGCCTCCAGC TGTGGGGAAT 
TTTAACTCCA ATGACAATAC TGTGTTTTCC CTAATTCAAG TCCCACCCGA GGCTTCTAAT 
CCACCTAATT ACAAATCTGC TGCTACTAAT GATAAATTCT CTGAATCTCT TCACAATCTG 
AGTTCCATTT TGCAGCGGCA ATCATCAGCA AATCAAATGC CCCTCAATTT AGCTGCGGAA 
AGTCAAAAGG CACTTAAGGG TGAAAACGCG ACTGTCGATC TTGCAAATAA AAACCCGATT 
CCAGCTGCCC TTCCGACGGG GCCACGTTTC CCTGCCAATC CAAACGGCAA GCCAAATTAT 
CATGCAGAGA AGACCGTCGC TCTAGATTTT ACTGTCCCTC GTGCGCCAGT CAGTTCTGTT 
GCCAATAACG TTGCTTCAAG TAACAGTCAG CCGGTTGCTG GAGGGCCGCT CTTTCCTAGG 
CCGTCTGTCA GCTATCAGAA TGGTCAGGCA GGCCTAGATG TTAACAAGCA GGGTTTTTAT 
CAGCAGGTCA CTAAGGATGG GCCCCCGCAG CAGCAGCCTG TTATGGATAT CCAACACCAG 
GGAGGAGTGG GCCTGGGGGA ATACATGGTC CCATCCTCTC AGCCACTGTC CTTGGTTCTG 
CCTGCACAAG CGCCTCCAGT GGGTAAATCG GTTCCATACC CTCCACAAGC TGAAAGCCAA 
GTTCAAACCT TGCAGAACCC TAGTGGAACT CCTTATAACA GTGCGCCAGA ACCTGCACCT 
AAATACGGAC CAACTGAACA GCCTTCATAT CCAGCTCCGC CTGTTCCTTC CTCATCAGCC 
ACAGTGAACC ACATAGCAAC ATATCAAGAG CAGCCCAGGC CTCCTGCCTC CCATACCCCT 
CAGCAAGCTG ATCCTTATTA CTACAGGTCT TACGGTGCCT ACCCACAGCC TTACCAACCC 
TTGGACCCAA GGGCAGCACC TCTTTACTAT CCGGACCCTT ATGGCGCTTA TGACCCCACC 
TACAGGCAGT ACAGTGGGTC TGCATATGTG AACCCTGGCA ATTACTCCTA TCAGGAGCCC 
GAACGTCCCA GTTCCAGATC CAGCCACTGC TCCGATCGCC CCTCCTCAAG GCAGGGCTAT 
AGCAGTAAAG GAGACTGGAA TGCACACAGT GGTTACTACT CAGGGTATTA TCAGAGTCAG 
TATGATTATG CAGACCCCAG CCGCTGGGAC CGATATCCTG GAGCCTATGA CCCTGGCTAT 
AGAGACCCCA GCAGTAATTA CTGGAATTAT ATGTACAACA GCCGTGATGA TGTGTACCAA 
AGGAACGTGC CTACTGCTTA TACCACCAGA TATGACGTTT ATGAAGACCG CTGGCAATAT 
GACCCACGTT ATGTGGGAGG CTTTGATGAA GAGCCACAGA GAGACCTCAC GAAGGACGAC 
TTTGACCGAC GTAGTGTTCA CAGTGAGCAT TCGGTCGATA GCATGCACAG CGGCCGGAGT 
GCACACAGTC GGAGAAGCAG TTTCAGCTCC CATTCACAAC ATAGCCAGAT CTACAAAAGC 
CAGCCAGACC TGTCTGCCAA CGCGTATGAA CCCCAAATAC AGAATCCTCC TCTCAATGAG 
TATTCGTATG GCGTGTACCC CAATGACTAC GGCACCCAGC AGGCGCTTGA TGATTACTCT 
TACGGGTATC CCAAGGGCAC AGAGTGGCAA CCTGTAGAAC AAGTGCCTTC AAGGCCGTTG 
ACTCCAGAGA AGTTTGGGAG CCCACACATC TGTGCCAGGT TTGGTTCTGG GGGTCAGTTA 
ATAAAGGTGC TGCCTAACCT TCCTTCAGAC GGACAGCCGG CCTTAGTGGA AATCCACAAC 
ATGGAGGTCA TTATGCAGAA CAGCGCAGAG CAGGAACAGC TGAGAAGTTT CCCTGGACCT 
TTAGTGAAGG ATGAAACTCA TAAAGTTGAC GTCATCAGTT TTGCCCAGAA GAAATTCAAG 
GAGTGTTCTC AGAATGAGAG CCTGTTAGAC CAAGAGTCTG CTTGCCTGCT CTGGGACTTC 
ATTGTGCTTC TGTGCCGGCA AAATGGGACT GTTGTTGGAA CAGATATCGC TGAGCTGCTT 
TTGAGAGATC ACAAGACAGT GTGGCTTCCT GCAAAGTCTC CCAACGAAGC CAACCTGATC 
GATTTCAGCA ATGAGCCGCT CGAGCAGCAG GAGGAAGAGT CAGGGGCTTC TCAGTTGTCC 
TTCCTCACGG ATTCTTTGCA AAGCACCGGG GAAGCTCTAG AGAAGGAGAC TGAGCGGTTC 
AGAGAGCTTC TACTTTTTGG GCGTAAAAAG GATGCCTTAG AATCTGCTAT GAAAAATGGA 
CTGTGGGGCC ACGCTTTGCT CCTAGCTAGC AAAATGGACA GCCGAACGCA CGCCAGAGTT 
ATGACAAGGT TTGCCAACAG CCTTCCAATT AACGATCCCC TGCAGACTGT GTATCAGCTC 
TTGTCTGGGA GAATGCCAGC AGCAGCCACG TGCTGCGGAG ATGAGAAATG GGGTGACTGG 
CGCCCTCACC TGGCGATGGT GCTGTCTAAT CTGACCAACA ATGTTGACGT TCCCACCAGG 
GCCATGACAA CAATGGGAGA TACATTAGCA TCCCGGGGGC TGATGGATGC CTCACACTTC 
TGCTATTTAA TGGCACAAGT CGGCTTTGGC ATTTTCACCA AGAAATCTAC TAAGTTGGTC 
CTCATTGGAT CCAACCACAG TTTATCATTT GCAAAGTTTT GCACTAACGA AGCCATCCAG 
AGAACAGAGG CTTATGAATA TGCACAGGCA CTTGGCACCC AGTCTTTCTC TCTCCCGAAT 
ATACAGGTTT ACAAGTTCAT CTACGCTTGC CGTTTGGCGG AACACGGATT ATCTGCACAA 
GCTTTCCATT ACTGTGAAGT CATCTCCAAG ACCATCTTGA AGCACCCGTC TTGCTACTCC 
CCTGTGCTTG TCAGCCAGCT ACTGGAGGTG TCCTCCCACT TGAGGTTCTT TGACCCACAG 
CTGAAGGAGA AGCCAGAACA GGAGTTATTT ATCGACCCTC CTTGGCTGCA GCATCTCCGC 
ACCTTGGATA AGCAAATGAA GCATGGTACT GTAGTTTATG ACATGGGCAG AACCACACCT 
CAGCAGTTTG CCTGCAGTAC ACCAAGCTCG GAGATAGACC ATGTGAGCCA ATCAGATGGT 
TTTGGAATAG CCGGGGATAT ACCAACTCAT ACAGAGAATC CATTACTGGC CAATCTCCTG 
CCCAACATGA TGCCTCCAGT ACAAGCTGTG CTAGTTCCTG CTGGTCCTGA AAATCCGCCA 
CCTGTATATC CACCCTACAC AGGGAATGAT CCCTCCTACC CAGGTACACC TCAGGGTCTA 
GCTCCCTCAT CTGGGCCTCC AACTCCATCT ACTGCTCCAA ATTTTGTGCC GTTTGGTGTG 
GAACAGGCAA CTGTTTATCC ACCTGCTGCT CTACAGCCAT ACCCACCTCT TCCCCAGGCA 
ACAGATTGTG CTCCTCCAAT GCAGATGAAT CAAGATCCAG GACTGCAAAG GATTCAGCAG 
AATTCCCCTA CCAAAACCAC CTTCGCTGAT CCTGAAGATT TCTACGGTGT CATGGCAAAA 
ATGGTTCCCG GGCAGAGATC ACGGACAGTG TCACAGTCCT CATCACACAT GAGGCGGACA 
CGGACAACCT CAGAGTCATC AACCCATTCT ATTGGGTCTG GACGTAGAAG TTCCATGGGC 
ATCCAACCAT CGCCTCCTCC TGCCTCTATC CCAGAGGCAG ACAGACCAGA AGAGAAGAAC 
AAACATAAGT CTCATGGTCC ATCCCAAGGG AGTGGTAAAA GTTGGTTTCC CCTGAAATTT 
GGCTGGTTGA TGGGAAAAGG CAAGAACGAA GCACACTTAC CTGATGACAG GAATAAATCT 
ATTGTGTGGG ATGAGAAGCA GCAGCGGTGG ACTAACCTGG ATGAACCAGA AGAAGAGAGA 
AAACCGTTGC CTCCACCACC ACCAGGGATG CTGAAAAAAC CACAGACTGC TCCATCTGTC 
CCAGGTGGTC CTCTCAACCC TTCTGTCAAT GTTTTCTCCA TCAGAGCAGC AGGGGCAAGA 
GGTCGTTACA TTGATGTCTT AAACCCAGGG GGTACCCGGC CCACTAACTC GGTCCCTCCG 
CCTGCTGATT TGTTTGCACC TTTGGCCCCC ATGCCTATCC CCACGAACCT GTTTGTTCCA 
AATGCAGTCC CAGAAGAGGG TCAGCCTGCG GAAGCCAGTG GGGCAGAAAT GGTGCAACCT 
GCGGGTCAGA CGGGGCTCAG TAACTTTTCC CAGGCACAGT TACCCGGTTT GCCCCCTGTG 
TCTGATCTTC CAACAAATCA GGAAGATGGG CAGATGGGAG AGCTTTCCCG ATCTAGCTCT 
CTCAGTTCCT TATCCCGTGA AGTAAGCCAG CATATGGAGC AGCTCTCTGC CTCTCCCAGG 
AACTCTACTG CAACAGCAGC ACCTTCTGGC TCTCCAACTG TGGCTGAACT ACAGTTCAGG 
ATCACTGTTA GCTGCTCCCT TCTACTCTGC AGCTGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC100037049 ( XM_018232581.1 ) cDNA ORF clone, Xenopus laevis(African clawed frog) -> XP_018088070.1 Xenopus laevis protein transport protein Sec16A (LOC100037049), transcript variant X3, mRNA.

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CloneID	OAf43841
Clone ID Related Accession (Same CDS sequence)	XM_018232581.1
Accession Version	XM_018232581.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7035bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1474214400000
Organism	Xenopus laevis(African clawed frog)
Product	protein transport protein Sec16A isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030739.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus laevis Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAGCCCC CTCCGCAAGC AAGGCATCCT GGCACGGGTG GACCTCCTCG CATGGGACTT 
GGCAATAATG CCCACTGGCG CAAAAGGGGA CCTAATCCTG TACCAGTAGC TTCAGCTTCA 
GTGCAGCCAG TAACAGACCC TTTTGCCTTT GGCCGTATTA CCCCACAAAG TATGGCACAG 
GGCAATGTCC CGATAGGAAA CCCACTGATG GCACCCAGTG TTTCCCCTTC TTTTTACCCT 
CAACCCGTTC CTGTACATGC ATCGGCTGCT GTAGATCAAG GGCATGGGTC TCCTGCATCA 
CTACCAGGAT TTGTTCCACA ACCTGCTGCT ACGGGGGGGA TGTATACAAA TGTGTCTGTC 
CCATCACCAT CAAGTGCCCC CTTTCACAAC TACCCAGCCG CCACCTCTAG TGCTCAGCCT 
GGACTCAGTA ATATTCAGAC TTTTCAGAAT ACAGCAAGTG GACTTGAAAA TGCCTTTCTG 
CCAGTGCACA TCAGGGCTCA GTCAACACAA GGCATTAATT ATAACCCTCC ATCTTCATCA 
CCTTTGCAGC ACTTTATCCC TGCACCATCT CAGCCAACTA ATGCTCCCTC TGGGTCCTTA 
CAAAGTGTAC CTCCTGCCTC AGCTCAGAAC TTCCCCCTTC CTGTTCAACA GCCATCCCAT 
AATGCTCCTT ATTACTCCCC ACCTTATTTA GAAGTGTCTA ATCTGGTCCC TCAAGCCAAC 
GAAACAGTGG CACAGCAGAG GTCAGCTCCT GGGCATTACC CGCCTGCCAC TGTCGCCAAT 
CCTACATTTC AACACAGCAC TTCACAACCC ACCATTTTAA ATCCGATAGA CTATATGCAC 
CGCCACAATC CTGACCAGAA CTGGGTTAAG CAACCTCACA AGGATCAGCA GCATCCACCT 
TTTCCCAGTC CTAACCCCGT ACAATACCCA ACTCAGCCAC CTGAGCCCCA TCAGGAGGTG 
ACTAATGCAT CTGGTCATGT CTCCACCAAT GTCGACTCTG GTACTATATC TATGTTTTTT 
AAAGGGGATG AAGAAGAAAA TGTAGAAATA GTTTCTTCCG ACCAGCAGAA TGCATCTGTT 
AAAGTGGATC ATGGTTCTTC TCATTACCAG AACTCTGGAC ATGAAGGTGT GCCTTCTCTC 
CTGCCACAGC CCACAGTCAG CAGCATGTCG TCACTTACAC CAATCTCTGT GTCTCAGAAT 
GTTGGCAATT TGACCCAACC ACATTCAGAT TGGAGGCAGG ATGTGTTTCC TGTTGGCGAT 
AAAATTTCTA AACCCAAAGT TGGAAGTGAA TTGCCCTATG ACAATGTTGA AAACATAGAG 
TGTGTACAAA ACCAAGAGGT TCCCCCCAAC GAGCCCAATC ATTTTAACCT TTATCCTTCT 
AATGTAAATT CAGATACGTA CCGCTATGGG TCCATGCCTG GACTCTCCCT ACAAAAGAAT 
GTTAGCAGCC AACCTGAAGC TGGTCCGAAT CTAGAGACGC CTGTCACAGT GGGTTGTCCT 
GTCAGATCTG ATAGTGTTTC ATCAAATTAT AGCAACCTGA GTCACAAAAG CCTGCCTGTG 
CCCACCCGGC CTCAAGAGCT AGCCGGGACA TTTATTCAGA AGGAGAGAGG AAGGCTTGAA 
GGTGACTCCT CCCATGGTTT CTTTAAGCAA ATTGATTCTT TGCCCATGGC AGGTGATGCT 
GTAGGCCAGA ATATGGGTCA GCACACGTTC CACAGCAATT TATCCCAGCC ACCAACTCCA 
AGTCCTCCTA AACCTACTGG GATCTTCATG ACGAGTGCAA ATAGTTCTTT CGAACCTGTT 
AGATCGCACA GCATTGGTTT GAAAGCGTCC GACGTAGACC AATTGAAAAT GGGTGGAGAC 
TTAAGAGAGG GGCATAATGT TCCACCAAGC GCCTCTCTCC CAGCAGCTTC CCCTGGTAAC 
TTGGAACAAC CACCAGATAA CCTGGAATCC ATATTCTTAG GTAAAAGAAA TTCTCACGGA 
AACATTACCA GCAATGGGAA CTTGTTCCAT TCTGCTGGCG ACCCGCCATT GGATAATATA 
TCCTTACTGC TTGAGAGACG TCCATCGTCA AGAGCACACG GGTCTAACAA GAAATGCGAA 
AGCCCGGCAA CCACCCTGTG GGCTCAAGGT GAGCTTCCAA ATTTTGGCGG AAATGTTTTG 
TTGGCTCCTG CAGCTCCTTC ACTCTATGTG CCCCCTAAAC CCCAAACCGA AATAATTCAG 
CCACCTGAAG ATGGGTCTTA TGATTCATGC TCGGGTGGAA CAGATATGGG GAAATTTCAG 
ACCTCTCTGG AAGAACAAAT TTCTTCGGAA AACCTTGAGA ATCCTCCAAA GATCACTGAT 
GAGGAGCAAC TTCCCTCGCA GGCAAGTTCA GGTTACGCCA GTTTGTTGTC TTCGCCGCCC 
ACTGAGGTAC TTCAGAATCA GCCCTTGCTA ATTGCCCAAC CAAATAAAAC CTATAATTTG 
TCACAGCCTA ATAATTTTTC ATTGTCGTTA CCAAATAAGG TAAAAAATAT TAATTCGCTT 
AAGGAACTGG GAGCAGCTGA CAAGCCGGGT CCTCAGAGTT TGCCTCCAGC TGTGGGGAAT 
TTTAACTCCA ATGACAATAC TGTGTTTTCC CTAATTCAAG TCCCACCCGA GGCTTCTAAT 
CCACCTAATT ACAAATCTGC TGCTACTAAT GATAAATTCT CTGAATCTCT TCACAATCTG 
AGTTCCATTT TGCAGCGGCA ATCATCAGCA AATCAAATGC CCCTCAATTT AGCTGCGGAA 
AGTCAAAAGG CACTTAAGGG TGAAAACGCG ACTGTCGATC TTGCAAATAA AAACCCGATT 
CCAGCTGCCC TTCCGACGGG GCCACGTTTC CCTGCCAATC CAAACGGCAA GCCAAATTAT 
CATGCAGAGA AGACCGTCGC TCTAGATTTT ACTGTCCCTC GTGCGCCAGT CAGTTCTGTT 
GCCAATAACG TTGCTTCAAG TAACAGTCAG CCGGTTGCTG GAGGGCCGCT CTTTCCTAGG 
CCGTCTGTCA GCTATCAGAA TGGTCAGGCA GGCCTAGATG TTAACAAGCA GGGTTTTTAT 
CAGCAGGTCA CTAAGGATGG GCCCCCGCAG CAGCAGCCTG TTATGGATAT CCAACACCAG 
GGAGGAGTGG GCCTGGGGGA ATACATGGTC CCATCCTCTC AGCCACTGTC CTTGGTTCTG 
CCTGCACAAG CGCCTCCAGT GGGTAAATCG GTTCCATACC CTCCACAAGC TGAAAGCCAA 
GTTCAAACCT TGCAGAACCC TAGTGGAACT CCTTATAACA GTGCGCCAGA ACCTGCACCT 
AAATACGGAC CAACTGAACA GCCTTCATAT CCAGCTCCGC CTGTTCCTTC CTCATCAGCC 
ACAGTGAACC ACATAGCAAC ATATCAAGAG CAGCCCAGGC CTCCTGCCTC CCATACCCCT 
CAGCAAGCTG ATCCTTATTA CTACAGGTCT TACGGTGCCT ACCCACAGCC TTACCAACCC 
TTGGACCCAA GGGCAGCACC TCTTTACTAT CCGGACCCTT ATGGCGCTTA TGACCCCACC 
TACAGGCAGT ACAGTGGGTC TGCATATGTG AACCCTGGCA ATTACTCCTA TCAGGAGCCC 
GAACGTCCCA GTTCCAGATC CAGCCACTGC TCCGATCGCC CCTCCTCAAG GCAGGGCTAT 
AGCAGTAAAG GAGACTGGAA TGCACACAGT GGTTACTACT CAGGGTATTA TCAGAGTCAG 
TATGATTATG CAGACCCCAG CCGCTGGGAC CGATATCCTG GAGCCTATGA CCCTGGCTAT 
AGAGACCCCA GCAGTAATTA CTGGAATTAT ATGTACAACA GCCGTGATGA TGTGTACCAA 
AGGAACGTGC CTACTGCTTA TACCACCAGA TATGACGTTT ATGAAGACCG CTGGCAATAT 
GACCCACGTT ATGTGGGAGG CTTTGATGAA GAGCCACAGA GAGACCTCAC GAAGGACGAC 
TTTGACCGAC GTAGTGTTCA CAGTGAGCAT TCGGTCGATA GCATGCACAG CGGCCGGAGT 
GCACACAGTC GGAGAAGCAG TTTCAGCTCC CATTCACAAC ATAGCCAGAT CTACAAAAGC 
CAGCCAGACC TGTCTGCCAA CGCGTATGAA CCCCAAATAC AGAATCCTCC TCTCAATGAG 
TATTCGTATG GCGTGTACCC CAATGACTAC GGCACCCAGC AGGCGCTTGA TGATTACTCT 
TACGGGTATC CCAAGGGCAC AGAGTGGCAA CCTGTAGAAC AAGTGCCTTC AAGGCCGTTG 
ACTCCAGAGA AGTTTGGGAG CCCACACATC TGTGCCAGGT TTGGTTCTGG GGGTCAGTTA 
ATAAAGGTGC TGCCTAACCT TCCTTCAGAC GGACAGCCGG CCTTAGTGGA AATCCACAAC 
ATGGAGGTCA TTATGCAGAA CAGCGCAGAG CAGGAACAGC TGAGAAGTTT CCCTGGACCT 
TTAGTGAAGG ATGAAACTCA TAAAGTTGAC GTCATCAGTT TTGCCCAGAA GAAATTCAAG 
GAGTGTTCTC AGAATGAGAG CCTGTTAGAC CAAGAGTCTG CTTGCCTGCT CTGGGACTTC 
ATTGTGCTTC TGTGCCGGCA AAATGGGACT GTTGTTGGAA CAGATATCGC TGAGCTGCTT 
TTGAGAGATC ACAAGACAGT GTGGCTTCCT GCAAAGTCTC CCAACGAAGC CAACCTGATC 
GATTTCAGCA ATGAGCCGCT CGAGCAGCAG GAGGAAGAGT CAGGGGCTTC TCAGTTGTCC 
TTCCTCACGG ATTCTTTGCA AAGCACCGGG GAAGCTCTAG AGAAGGAGAC TGAGCGGTTC 
AGAGAGCTTC TACTTTTTGG GCGTAAAAAG GATGCCTTAG AATCTGCTAT GAAAAATGGA 
CTGTGGGGCC ACGCTTTGCT CCTAGCTAGC AAAATGGACA GCCGAACGCA CGCCAGAGTT 
ATGACAAGGT TTGCCAACAG CCTTCCAATT AACGATCCCC TGCAGACTGT GTATCAGCTC 
TTGTCTGGGA GAATGCCAGC AGCAGCCACG TGCTGCGGAG ATGAGAAATG GGGTGACTGG 
CGCCCTCACC TGGCGATGGT GCTGTCTAAT CTGACCAACA ATGTTGACGT TCCCACCAGG 
GCCATGACAA CAATGGGAGA TACATTAGCA TCCCGGGGGC TGATGGATGC CTCACACTTC 
TGCTATTTAA TGGCACAAGT CGGCTTTGGC ATTTTCACCA AGAAATCTAC TAAGTTGGTC 
CTCATTGGAT CCAACCACAG TTTATCATTT GCAAAGTTTT GCACTAACGA AGCCATCCAG 
AGAACAGAGG CTTATGAATA TGCACAGGCA CTTGGCACCC AGTCTTTCTC TCTCCCGAAT 
ATACAGGTTT ACAAGTTCAT CTACGCTTGC CGTTTGGCGG AACACGGATT ATCTGCACAA 
GCTTTCCATT ACTGTGAAGT CATCTCCAAG ACCATCTTGA AGCACCCGTC TTGCTACTCC 
CCTGTGCTTG TCAGCCAGCT ACTGGAGGTG TCCTCCCACT TGAGGTTCTT TGACCCACAG 
CTGAAGGAGA AGCCAGAACA GGAGTTATTT ATCGACCCTC CTTGGCTGCA GCATCTCCGC 
ACCTTGGATA AGCAAATGAA GCATGGTACT GTAGTTTATG ACATGGGCAG AACCACACCT 
CAGCAGTTTG CCTGCAGTAC ACCAAGCTCG GAGATAGACC ATGTGAGCCA ATCAGATGGT 
TTTGGAATAG CCGGGGATAT ACCAACTCAT ACAGAGAATC CATTACTGGC CAATCTCCTG 
CCCAACATGA TGCCTCCAGT ACAAGCTGTG CTAGTTCCTG CTGGTCCTGA AAATCCGCCA 
CCTGTATATC CACCCTACAC AGGGAATGAT CCCTCCTACC CAGGTACACC TCAGGGTCTA 
GCTCCCTCAT CTGGGCCTCC AACTCCATCT ACTGCTCCAA ATTTTGTGCC GTTTGGTGTG 
GAACAGGCAA CTGTTTATCC ACCTGCTGCT CTACAGCCAT ACCCACCTCT TCCCCAGGCA 
ACAGATTGTG CTCCTCCAAT GCAGATGAAT CAAGATCCAG GACTGCAAAG GATTCAGCAG 
AATTCCCCTA CCAAAACCAC CTTCGCTGAT CCTGAAGATT TCTACGGTGT CATGGCAAAA 
ATGGTTCCCG GGCAGAGATC ACGGACAGTG TCACAGTCCT CATCACACAT GAGGCGGACA 
CGGACAACCT CAGAGTCATC AACCCATTCT ATTGGGTCTG GACGTAGAAG TTCCATGGGC 
ATCCAACCAT CGCCTCCTCC TGCCTCTATC CCAGAGGCAG ACAGACCAGA AGAGAAGAAC 
AAACATAAGT CTCATGGTCC ATCCCAAGGG AGTGGTAAAA GTTGGTTTCC CCTGAAATTT 
GGCTGGTTGA TGGGAAAAGG CAAGAACGAA GCACACTTAC CTGATGACAG GAATAAATCT 
ATTGTGTGGG ATGAGAAGCA GCAGCGGTGG ACTAACCTGG ATGAACCAGA AGAAGAGAGA 
AAACCGTTGC CTCCACCACC ACCAGGGATG CTGAAAAAAC CACAGACTGC TCCATCTGTC 
CCAGGTGGTC CTCTCAACCC TTCTGTCAAT GTTTTCTCCA TCAGAGCAGC AGGGGCAAGA 
GGTCGTTACA TTGATGTCTT AAACCCAGGG GGTACCCGGC CCACTAACTC GGTCCCTCCG 
CCTGCTGATT TGTTTGCACC TTTGGCCCCC ATGCCTATCC CCACGAACCT GTTTGTTCCA 
AATGCAGTCC CAGAAGAGGG TCAGCCTGCG GAAGCCAGTG GGGCAGAAAT GGTGCAACCT 
GCGGGTCAGA CGGGGCTCAG TAACTTTTCC CAGGCACAGT TACCCGGTTT GCCCCCTGTG 
TCTGATCTTC CAACAAATCA GGAAGATGGG CAGATGGGAG AGGCTCACGT GGCCCCTGCG 
TCAGGCCCCC CACCTACAGG GGCTGTCCCC TTCTACAACC CCTCCCAGTT TTCACAACAG 
CAGCCTCCTT CTGCCCAAGC AGGTGGGCTA CGACCTGGAC GTCTCGGCCA GAGGAAATAT 
CCTTCCTTAA AATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_018088070.1
CDS	214..7248
Translation	MQPPPQARHPGTGGPPRMGLGNNAHWRKRGPNPVPVASASVQPVTDPFAFGRITPQSMAQGNVPIGNPLMAPSVSPSFYPQPVPVHASAAVDQGHGSPASLPGFVPQPAATGGMYTNVSVPSPSSAPFHNYPAATSSAQPGLSNIQTFQNTASGLENAFLPVHIRAQSTQGINYNPPSSSPLQHFIPAPSQPTNAPSGSLQSVPPASAQNFPLPVQQPSHNAPYYSPPYLEVSNLVPQANETVAQQRSAPGHYPPATVANPTFQHSTSQPTILNPIDYMHRHNPDQNWVKQPHKDQQHPPFPSPNPVQYPTQPPEPHQEVTNASGHVSTNVDSGTISMFFKGDEEENVEIVSSDQQNASVKVDHGSSHYQNSGHEGVPSLLPQPTVSSMSSLTPISVSQNVGNLTQPHSDWRQDVFPVGDKISKPKVGSELPYDNVENIECVQNQEVPPNEPNHFNLYPSNVNSDTYRYGSMPGLSLQKNVSSQPEAGPNLETPVTVGCPVRSDSVSSNYSNLSHKSLPVPTRPQELAGTFIQKERGRLEGDSSHGFFKQIDSLPMAGDAVGQNMGQHTFHSNLSQPPTPSPPKPTGIFMTSANSSFEPVRSHSIGLKASDVDQLKMGGDLREGHNVPPSASLPAASPGNLEQPPDNLESIFLGKRNSHGNITSNGNLFHSAGDPPLDNISLLLERRPSSRAHGSNKKCESPATTLWAQGELPNFGGNVLLAPAAPSLYVPPKPQTEIIQPPEDGSYDSCSGGTDMGKFQTSLEEQISSENLENPPKITDEEQLPSQASSGYASLLSSPPTEVLQNQPLLIAQPNKTYNLSQPNNFSLSLPNKVKNINSLKELGAADKPGPQSLPPAVGNFNSNDNTVFSLIQVPPEASNPPNYKSAATNDKFSESLHNLSSILQRQSSANQMPLNLAAESQKALKGENATVDLANKNPIPAALPTGPRFPANPNGKPNYHAEKTVALDFTVPRAPVSSVANNVASSNSQPVAGGPLFPRPSVSYQNGQAGLDVNKQGFYQQVTKDGPPQQQPVMDIQHQGGVGLGEYMVPSSQPLSLVLPAQAPPVGKSVPYPPQAESQVQTLQNPSGTPYNSAPEPAPKYGPTEQPSYPAPPVPSSSATVNHIATYQEQPRPPASHTPQQADPYYYRSYGAYPQPYQPLDPRAAPLYYPDPYGAYDPTYRQYSGSAYVNPGNYSYQEPERPSSRSSHCSDRPSSRQGYSSKGDWNAHSGYYSGYYQSQYDYADPSRWDRYPGAYDPGYRDPSSNYWNYMYNSRDDVYQRNVPTAYTTRYDVYEDRWQYDPRYVGGFDEEPQRDLTKDDFDRRSVHSEHSVDSMHSGRSAHSRRSSFSSHSQHSQIYKSQPDLSANAYEPQIQNPPLNEYSYGVYPNDYGTQQALDDYSYGYPKGTEWQPVEQVPSRPLTPEKFGSPHICARFGSGGQLIKVLPNLPSDGQPALVEIHNMEVIMQNSAEQEQLRSFPGPLVKDETHKVDVISFAQKKFKECSQNESLLDQESACLLWDFIVLLCRQNGTVVGTDIAELLLRDHKTVWLPAKSPNEANLIDFSNEPLEQQEEESGASQLSFLTDSLQSTGEALEKETERFRELLLFGRKKDALESAMKNGLWGHALLLASKMDSRTHARVMTRFANSLPINDPLQTVYQLLSGRMPAAATCCGDEKWGDWRPHLAMVLSNLTNNVDVPTRAMTTMGDTLASRGLMDASHFCYLMAQVGFGIFTKKSTKLVLIGSNHSLSFAKFCTNEAIQRTEAYEYAQALGTQSFSLPNIQVYKFIYACRLAEHGLSAQAFHYCEVISKTILKHPSCYSPVLVSQLLEVSSHLRFFDPQLKEKPEQELFIDPPWLQHLRTLDKQMKHGTVVYDMGRTTPQQFACSTPSSEIDHVSQSDGFGIAGDIPTHTENPLLANLLPNMMPPVQAVLVPAGPENPPPVYPPYTGNDPSYPGTPQGLAPSSGPPTPSTAPNFVPFGVEQATVYPPAALQPYPPLPQATDCAPPMQMNQDPGLQRIQQNSPTKTTFADPEDFYGVMAKMVPGQRSRTVSQSSSHMRRTRTTSESSTHSIGSGRRSSMGIQPSPPPASIPEADRPEEKNKHKSHGPSQGSGKSWFPLKFGWLMGKGKNEAHLPDDRNKSIVWDEKQQRWTNLDEPEEERKPLPPPPPGMLKKPQTAPSVPGGPLNPSVNVFSIRAAGARGRYIDVLNPGGTRPTNSVPPPADLFAPLAPMPIPTNLFVPNAVPEEGQPAEASGAEMVQPAGQTGLSNFSQAQLPGLPPVSDLPTNQEDGQMGEAHVAPASGPPPTGAVPFYNPSQFSQQQPPSAQAGGLRPGRLGQRKYPSLK

Target ORF information:

RefSeq Version	XM_018232581.1
Organism	Xenopus laevis(African clawed frog)
Definition	Xenopus laevis protein transport protein Sec16A (LOC100037049), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_018232581.1

ORF Insert Sequence:

ATGCAGCCCC CTCCGCAAGC AAGGCATCCT GGCACGGGTG GACCTCCTCG CATGGGACTT 
GGCAATAATG CCCACTGGCG CAAAAGGGGA CCTAATCCTG TACCAGTAGC TTCAGCTTCA 
GTGCAGCCAG TAACAGACCC TTTTGCCTTT GGCCGTATTA CCCCACAAAG TATGGCACAG 
GGCAATGTCC CGATAGGAAA CCCACTGATG GCACCCAGTG TTTCCCCTTC TTTTTACCCT 
CAACCCGTTC CTGTACATGC ATCGGCTGCT GTAGATCAAG GGCATGGGTC TCCTGCATCA 
CTACCAGGAT TTGTTCCACA ACCTGCTGCT ACGGGGGGGA TGTATACAAA TGTGTCTGTC 
CCATCACCAT CAAGTGCCCC CTTTCACAAC TACCCAGCCG CCACCTCTAG TGCTCAGCCT 
GGACTCAGTA ATATTCAGAC TTTTCAGAAT ACAGCAAGTG GACTTGAAAA TGCCTTTCTG 
CCAGTGCACA TCAGGGCTCA GTCAACACAA GGCATTAATT ATAACCCTCC ATCTTCATCA 
CCTTTGCAGC ACTTTATCCC TGCACCATCT CAGCCAACTA ATGCTCCCTC TGGGTCCTTA 
CAAAGTGTAC CTCCTGCCTC AGCTCAGAAC TTCCCCCTTC CTGTTCAACA GCCATCCCAT 
AATGCTCCTT ATTACTCCCC ACCTTATTTA GAAGTGTCTA ATCTGGTCCC TCAAGCCAAC 
GAAACAGTGG CACAGCAGAG GTCAGCTCCT GGGCATTACC CGCCTGCCAC TGTCGCCAAT 
CCTACATTTC AACACAGCAC TTCACAACCC ACCATTTTAA ATCCGATAGA CTATATGCAC 
CGCCACAATC CTGACCAGAA CTGGGTTAAG CAACCTCACA AGGATCAGCA GCATCCACCT 
TTTCCCAGTC CTAACCCCGT ACAATACCCA ACTCAGCCAC CTGAGCCCCA TCAGGAGGTG 
ACTAATGCAT CTGGTCATGT CTCCACCAAT GTCGACTCTG GTACTATATC TATGTTTTTT 
AAAGGGGATG AAGAAGAAAA TGTAGAAATA GTTTCTTCCG ACCAGCAGAA TGCATCTGTT 
AAAGTGGATC ATGGTTCTTC TCATTACCAG AACTCTGGAC ATGAAGGTGT GCCTTCTCTC 
CTGCCACAGC CCACAGTCAG CAGCATGTCG TCACTTACAC CAATCTCTGT GTCTCAGAAT 
GTTGGCAATT TGACCCAACC ACATTCAGAT TGGAGGCAGG ATGTGTTTCC TGTTGGCGAT 
AAAATTTCTA AACCCAAAGT TGGAAGTGAA TTGCCCTATG ACAATGTTGA AAACATAGAG 
TGTGTACAAA ACCAAGAGGT TCCCCCCAAC GAGCCCAATC ATTTTAACCT TTATCCTTCT 
AATGTAAATT CAGATACGTA CCGCTATGGG TCCATGCCTG GACTCTCCCT ACAAAAGAAT 
GTTAGCAGCC AACCTGAAGC TGGTCCGAAT CTAGAGACGC CTGTCACAGT GGGTTGTCCT 
GTCAGATCTG ATAGTGTTTC ATCAAATTAT AGCAACCTGA GTCACAAAAG CCTGCCTGTG 
CCCACCCGGC CTCAAGAGCT AGCCGGGACA TTTATTCAGA AGGAGAGAGG AAGGCTTGAA 
GGTGACTCCT CCCATGGTTT CTTTAAGCAA ATTGATTCTT TGCCCATGGC AGGTGATGCT 
GTAGGCCAGA ATATGGGTCA GCACACGTTC CACAGCAATT TATCCCAGCC ACCAACTCCA 
AGTCCTCCTA AACCTACTGG GATCTTCATG ACGAGTGCAA ATAGTTCTTT CGAACCTGTT 
AGATCGCACA GCATTGGTTT GAAAGCGTCC GACGTAGACC AATTGAAAAT GGGTGGAGAC 
TTAAGAGAGG GGCATAATGT TCCACCAAGC GCCTCTCTCC CAGCAGCTTC CCCTGGTAAC 
TTGGAACAAC CACCAGATAA CCTGGAATCC ATATTCTTAG GTAAAAGAAA TTCTCACGGA 
AACATTACCA GCAATGGGAA CTTGTTCCAT TCTGCTGGCG ACCCGCCATT GGATAATATA 
TCCTTACTGC TTGAGAGACG TCCATCGTCA AGAGCACACG GGTCTAACAA GAAATGCGAA 
AGCCCGGCAA CCACCCTGTG GGCTCAAGGT GAGCTTCCAA ATTTTGGCGG AAATGTTTTG 
TTGGCTCCTG CAGCTCCTTC ACTCTATGTG CCCCCTAAAC CCCAAACCGA AATAATTCAG 
CCACCTGAAG ATGGGTCTTA TGATTCATGC TCGGGTGGAA CAGATATGGG GAAATTTCAG 
ACCTCTCTGG AAGAACAAAT TTCTTCGGAA AACCTTGAGA ATCCTCCAAA GATCACTGAT 
GAGGAGCAAC TTCCCTCGCA GGCAAGTTCA GGTTACGCCA GTTTGTTGTC TTCGCCGCCC 
ACTGAGGTAC TTCAGAATCA GCCCTTGCTA ATTGCCCAAC CAAATAAAAC CTATAATTTG 
TCACAGCCTA ATAATTTTTC ATTGTCGTTA CCAAATAAGG TAAAAAATAT TAATTCGCTT 
AAGGAACTGG GAGCAGCTGA CAAGCCGGGT CCTCAGAGTT TGCCTCCAGC TGTGGGGAAT 
TTTAACTCCA ATGACAATAC TGTGTTTTCC CTAATTCAAG TCCCACCCGA GGCTTCTAAT 
CCACCTAATT ACAAATCTGC TGCTACTAAT GATAAATTCT CTGAATCTCT TCACAATCTG 
AGTTCCATTT TGCAGCGGCA ATCATCAGCA AATCAAATGC CCCTCAATTT AGCTGCGGAA 
AGTCAAAAGG CACTTAAGGG TGAAAACGCG ACTGTCGATC TTGCAAATAA AAACCCGATT 
CCAGCTGCCC TTCCGACGGG GCCACGTTTC CCTGCCAATC CAAACGGCAA GCCAAATTAT 
CATGCAGAGA AGACCGTCGC TCTAGATTTT ACTGTCCCTC GTGCGCCAGT CAGTTCTGTT 
GCCAATAACG TTGCTTCAAG TAACAGTCAG CCGGTTGCTG GAGGGCCGCT CTTTCCTAGG 
CCGTCTGTCA GCTATCAGAA TGGTCAGGCA GGCCTAGATG TTAACAAGCA GGGTTTTTAT 
CAGCAGGTCA CTAAGGATGG GCCCCCGCAG CAGCAGCCTG TTATGGATAT CCAACACCAG 
GGAGGAGTGG GCCTGGGGGA ATACATGGTC CCATCCTCTC AGCCACTGTC CTTGGTTCTG 
CCTGCACAAG CGCCTCCAGT GGGTAAATCG GTTCCATACC CTCCACAAGC TGAAAGCCAA 
GTTCAAACCT TGCAGAACCC TAGTGGAACT CCTTATAACA GTGCGCCAGA ACCTGCACCT 
AAATACGGAC CAACTGAACA GCCTTCATAT CCAGCTCCGC CTGTTCCTTC CTCATCAGCC 
ACAGTGAACC ACATAGCAAC ATATCAAGAG CAGCCCAGGC CTCCTGCCTC CCATACCCCT 
CAGCAAGCTG ATCCTTATTA CTACAGGTCT TACGGTGCCT ACCCACAGCC TTACCAACCC 
TTGGACCCAA GGGCAGCACC TCTTTACTAT CCGGACCCTT ATGGCGCTTA TGACCCCACC 
TACAGGCAGT ACAGTGGGTC TGCATATGTG AACCCTGGCA ATTACTCCTA TCAGGAGCCC 
GAACGTCCCA GTTCCAGATC CAGCCACTGC TCCGATCGCC CCTCCTCAAG GCAGGGCTAT 
AGCAGTAAAG GAGACTGGAA TGCACACAGT GGTTACTACT CAGGGTATTA TCAGAGTCAG 
TATGATTATG CAGACCCCAG CCGCTGGGAC CGATATCCTG GAGCCTATGA CCCTGGCTAT 
AGAGACCCCA GCAGTAATTA CTGGAATTAT ATGTACAACA GCCGTGATGA TGTGTACCAA 
AGGAACGTGC CTACTGCTTA TACCACCAGA TATGACGTTT ATGAAGACCG CTGGCAATAT 
GACCCACGTT ATGTGGGAGG CTTTGATGAA GAGCCACAGA GAGACCTCAC GAAGGACGAC 
TTTGACCGAC GTAGTGTTCA CAGTGAGCAT TCGGTCGATA GCATGCACAG CGGCCGGAGT 
GCACACAGTC GGAGAAGCAG TTTCAGCTCC CATTCACAAC ATAGCCAGAT CTACAAAAGC 
CAGCCAGACC TGTCTGCCAA CGCGTATGAA CCCCAAATAC AGAATCCTCC TCTCAATGAG 
TATTCGTATG GCGTGTACCC CAATGACTAC GGCACCCAGC AGGCGCTTGA TGATTACTCT 
TACGGGTATC CCAAGGGCAC AGAGTGGCAA CCTGTAGAAC AAGTGCCTTC AAGGCCGTTG 
ACTCCAGAGA AGTTTGGGAG CCCACACATC TGTGCCAGGT TTGGTTCTGG GGGTCAGTTA 
ATAAAGGTGC TGCCTAACCT TCCTTCAGAC GGACAGCCGG CCTTAGTGGA AATCCACAAC 
ATGGAGGTCA TTATGCAGAA CAGCGCAGAG CAGGAACAGC TGAGAAGTTT CCCTGGACCT 
TTAGTGAAGG ATGAAACTCA TAAAGTTGAC GTCATCAGTT TTGCCCAGAA GAAATTCAAG 
GAGTGTTCTC AGAATGAGAG CCTGTTAGAC CAAGAGTCTG CTTGCCTGCT CTGGGACTTC 
ATTGTGCTTC TGTGCCGGCA AAATGGGACT GTTGTTGGAA CAGATATCGC TGAGCTGCTT 
TTGAGAGATC ACAAGACAGT GTGGCTTCCT GCAAAGTCTC CCAACGAAGC CAACCTGATC 
GATTTCAGCA ATGAGCCGCT CGAGCAGCAG GAGGAAGAGT CAGGGGCTTC TCAGTTGTCC 
TTCCTCACGG ATTCTTTGCA AAGCACCGGG GAAGCTCTAG AGAAGGAGAC TGAGCGGTTC 
AGAGAGCTTC TACTTTTTGG GCGTAAAAAG GATGCCTTAG AATCTGCTAT GAAAAATGGA 
CTGTGGGGCC ACGCTTTGCT CCTAGCTAGC AAAATGGACA GCCGAACGCA CGCCAGAGTT 
ATGACAAGGT TTGCCAACAG CCTTCCAATT AACGATCCCC TGCAGACTGT GTATCAGCTC 
TTGTCTGGGA GAATGCCAGC AGCAGCCACG TGCTGCGGAG ATGAGAAATG GGGTGACTGG 
CGCCCTCACC TGGCGATGGT GCTGTCTAAT CTGACCAACA ATGTTGACGT TCCCACCAGG 
GCCATGACAA CAATGGGAGA TACATTAGCA TCCCGGGGGC TGATGGATGC CTCACACTTC 
TGCTATTTAA TGGCACAAGT CGGCTTTGGC ATTTTCACCA AGAAATCTAC TAAGTTGGTC 
CTCATTGGAT CCAACCACAG TTTATCATTT GCAAAGTTTT GCACTAACGA AGCCATCCAG 
AGAACAGAGG CTTATGAATA TGCACAGGCA CTTGGCACCC AGTCTTTCTC TCTCCCGAAT 
ATACAGGTTT ACAAGTTCAT CTACGCTTGC CGTTTGGCGG AACACGGATT ATCTGCACAA 
GCTTTCCATT ACTGTGAAGT CATCTCCAAG ACCATCTTGA AGCACCCGTC TTGCTACTCC 
CCTGTGCTTG TCAGCCAGCT ACTGGAGGTG TCCTCCCACT TGAGGTTCTT TGACCCACAG 
CTGAAGGAGA AGCCAGAACA GGAGTTATTT ATCGACCCTC CTTGGCTGCA GCATCTCCGC 
ACCTTGGATA AGCAAATGAA GCATGGTACT GTAGTTTATG ACATGGGCAG AACCACACCT 
CAGCAGTTTG CCTGCAGTAC ACCAAGCTCG GAGATAGACC ATGTGAGCCA ATCAGATGGT 
TTTGGAATAG CCGGGGATAT ACCAACTCAT ACAGAGAATC CATTACTGGC CAATCTCCTG 
CCCAACATGA TGCCTCCAGT ACAAGCTGTG CTAGTTCCTG CTGGTCCTGA AAATCCGCCA 
CCTGTATATC CACCCTACAC AGGGAATGAT CCCTCCTACC CAGGTACACC TCAGGGTCTA 
GCTCCCTCAT CTGGGCCTCC AACTCCATCT ACTGCTCCAA ATTTTGTGCC GTTTGGTGTG 
GAACAGGCAA CTGTTTATCC ACCTGCTGCT CTACAGCCAT ACCCACCTCT TCCCCAGGCA 
ACAGATTGTG CTCCTCCAAT GCAGATGAAT CAAGATCCAG GACTGCAAAG GATTCAGCAG 
AATTCCCCTA CCAAAACCAC CTTCGCTGAT CCTGAAGATT TCTACGGTGT CATGGCAAAA 
ATGGTTCCCG GGCAGAGATC ACGGACAGTG TCACAGTCCT CATCACACAT GAGGCGGACA 
CGGACAACCT CAGAGTCATC AACCCATTCT ATTGGGTCTG GACGTAGAAG TTCCATGGGC 
ATCCAACCAT CGCCTCCTCC TGCCTCTATC CCAGAGGCAG ACAGACCAGA AGAGAAGAAC 
AAACATAAGT CTCATGGTCC ATCCCAAGGG AGTGGTAAAA GTTGGTTTCC CCTGAAATTT 
GGCTGGTTGA TGGGAAAAGG CAAGAACGAA GCACACTTAC CTGATGACAG GAATAAATCT 
ATTGTGTGGG ATGAGAAGCA GCAGCGGTGG ACTAACCTGG ATGAACCAGA AGAAGAGAGA 
AAACCGTTGC CTCCACCACC ACCAGGGATG CTGAAAAAAC CACAGACTGC TCCATCTGTC 
CCAGGTGGTC CTCTCAACCC TTCTGTCAAT GTTTTCTCCA TCAGAGCAGC AGGGGCAAGA 
GGTCGTTACA TTGATGTCTT AAACCCAGGG GGTACCCGGC CCACTAACTC GGTCCCTCCG 
CCTGCTGATT TGTTTGCACC TTTGGCCCCC ATGCCTATCC CCACGAACCT GTTTGTTCCA 
AATGCAGTCC CAGAAGAGGG TCAGCCTGCG GAAGCCAGTG GGGCAGAAAT GGTGCAACCT 
GCGGGTCAGA CGGGGCTCAG TAACTTTTCC CAGGCACAGT TACCCGGTTT GCCCCCTGTG 
TCTGATCTTC CAACAAATCA GGAAGATGGG CAGATGGGAG AGGCTCACGT GGCCCCTGCG 
TCAGGCCCCC CACCTACAGG GGCTGTCCCC TTCTACAACC CCTCCCAGTT TTCACAACAG 
CAGCCTCCTT CTGCCCAAGC AGGTGGGCTA CGACCTGGAC GTCTCGGCCA GAGGAAATAT 
CCTTCCTTAA AATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC100037049 ( XM_018232582.1 ) cDNA ORF clone, Xenopus laevis(African clawed frog) -> XP_018088071.1 Xenopus laevis protein transport protein Sec16A (LOC100037049), transcript variant X4, mRNA.

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CloneID	OAf43842
Clone ID Related Accession (Same CDS sequence)	XM_018232582.1
Accession Version	XM_018232582.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7005bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1474214400000
Organism	Xenopus laevis(African clawed frog)
Product	protein transport protein Sec16A isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030739.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus laevis Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAGCCCC CTCCGCAAGC AAGGCATCCT GGCACGGGTG GACCTCCTCG CATGGGACTT 
GGCAATAATG CCCACTGGCG CAAAAGGGGA CCTAATCCTG TACCAGTAGC TTCAGCTTCA 
GTGCAGCCAG TAACAGACCC TTTTGCCTTT GGCCGTATTA CCCCACAAAG TATGGCACAG 
GGCAATGTCC CGATAGGAAA CCCACTGATG GCACCCAGTG TTTCCCCTTC TTTTTACCCT 
CAACCCGTTC CTGTACATGC ATCGGCTGCT GTAGATCAAG GGCATGGGTC TCCTGCATCA 
CTACCAGGAT TTGTTCCACA ACCTGCTGCT ACGGGGGGGA TGTATACAAA TGTGTCTGTC 
CCATCACCAT CAAGTGCCCC CTTTCACAAC TACCCAGCCG CCACCTCTAG TGCTCAGCCT 
GGACTCAGTA ATATTCAGAC TTTTCAGAAT ACAGCAAGTG GACTTGAAAA TGCCTTTCTG 
CCAGTGCACA TCAGGGCTCA GTCAACACAA GGCATTAATT ATAACCCTCC ATCTTCATCA 
CCTTTGCAGC ACTTTATCCC TGCACCATCT CAGCCAACTA ATGCTCCCTC TGGGTCCTTA 
CAAAGTGTAC CTCCTGCCTC AGCTCAGAAC TTCCCCCTTC CTGTTCAACA GCCATCCCAT 
AATGCTCCTT ATTACTCCCC ACCTTATTTA GAAGTGTCTA ATCTGGTCCC TCAAGCCAAC 
GAAACAGTGG CACAGCAGAG GTCAGCTCCT GGGCATTACC CGCCTGCCAC TGTCGCCAAT 
CCTACATTTC AACACAGCAC TTCACAACCC ACCATTTTAA ATCCGATAGA CTATATGCAC 
CGCCACAATC CTGACCAGAA CTGGGTTAAG CAACCTCACA AGGATCAGCA GCATCCACCT 
TTTCCCAGTC CTAACCCCGT ACAATACCCA ACTCAGCCAC CTGAGCCCCA TCAGGAGGTG 
ACTAATGCAT CTGGTCATGT CTCCACCAAT GTCGACTCTG GTACTATATC TATGTTTTTT 
AAAGGGGATG AAGAAGAAAA TGTAGAAATA GTTTCTTCCG ACCAGCAGAA TGCATCTGTT 
AAAGTGGATC ATGGTTCTTC TCATTACCAG AACTCTGGAC ATGAAGGTGT GCCTTCTCTC 
CTGCCACAGC CCACAGTCAG CAGCATGTCG TCACTTACAC CAATCTCTGT GTCTCAGAAT 
GTTGGCAATT TGACCCAACC ACATTCAGAT TGGAGGCAGG ATGTGTTTCC TGTTGGCGAT 
AAAATTTCTA AACCCAAAGT TGGAAGTGAA TTGCCCTATG ACAATGTTGA AAACATAGAG 
TGTGTACAAA ACCAAGAGGT TCCCCCCAAC GAGCCCAATC ATTTTAACCT TTATCCTTCT 
AATGTAAATT CAGATACGTA CCGCTATGGG TCCATGCCTG GACTCTCCCT ACAAAAGAAT 
GTTAGCAGCC AACCTGAAGC TGGTCCGAAT CTAGAGACGC CTGTCACAGT GGGTTGTCCT 
GTCAGATCTG ATAGTGTTTC ATCAAATTAT AGCAACCTGA GTCACAAAAG CCTGCCTGTG 
CCCACCCGGC CTCAAGAGCT AGCCGGGACA TTTATTCAGA AGGAGAGAGG AAGGCTTGAA 
GGTGACTCCT CCCATGGTTT CTTTAAGCAA ATTGATTCTT TGCCCATGGC AGGTGATGCT 
GTAGGCCAGA ATATGGGTCA GCACACGTTC CACAGCAATT TATCCCAGCC ACCAACTCCA 
AGTCCTCCTA AACCTACTGG GATCTTCATG ACGAGTGCAA ATAGTTCTTT CGAACCTGTT 
AGATCGCACA GCATTGGTTT GAAAGCGTCC GACGTAGACC AATTGAAAAT GGGTGGAGAC 
TTAAGAGAGG GGCATAATGT TCCACCAAGC GCCTCTCTCC CAGCAGCTTC CCCTGGTAAC 
TTGGAACAAC CACCAGATAA CCTGGAATCC ATATTCTTAG GTAAAAGAAA TTCTCACGGA 
AACATTACCA GCAATGGGAA CTTGTTCCAT TCTGCTGGCG ACCCGCCATT GGATAATATA 
TCCTTACTGC TTGAGAGACG TCCATCGTCA AGAGCACACG GGTCTAACAA GAAATGCGAA 
AGCCCGGCAA CCACCCTGTG GGCTCAAGGT GAGCTTCCAA ATTTTGGCGG AAATGTTTTG 
TTGGCTCCTG CAGCTCCTTC ACTCTATGTG CCCCCTAAAC CCCAAACCGA AATAATTCAG 
CCACCTGAAG ATGGGTCTTA TGATTCATGC TCGGGTGGAA CAGATATGGG GAAATTTCAG 
ACCTCTCTGG AAGAACAAAT TTCTTCGGAA AACCTTGAGA ATCCTCCAAA GATCACTGAT 
GAGGAGCAAC TTCCCTCGCA GGCAAGTTCA GGTTACGCCA GTTTGTTGTC TTCGCCGCCC 
ACTGAGGTAC TTCAGAATCA GCCCTTGCTA ATTGCCCAAC CAAATAAAAC CTATAATTTG 
TCACAGCCTA ATAATTTTTC ATTGTCGTTA CCAAATAAGG TAAAAAATAT TAATTCGCTT 
AAGGAACTGG GAGCAGCTGA CAAGCCGGGT CCTCAGAGTT TGCCTCCAGC TGTGGGGAAT 
TTTAACTCCA ATGACAATAC TGTGTTTTCC CTAATTCAAG TCCCACCCGA GGCTTCTAAT 
CCACCTAATT ACAAATCTGC TGCTACTAAT GATAAATTCT CTGAATCTCT TCACAATCTG 
AGTTCCATTT TGCAGCGGCA ATCATCAGCA AATCAAATGC CCCTCAATTT AGCTGCGGAA 
AGTCAAAAGG CACTTAAGGG TGAAAACGCG ACTGTCGATC TTGCAAATAA AAACCCGATT 
CCAGCTGCCC TTCCGACGGG GCCACGTTTC CCTGCCAATC CAAACGGCAA GCCAAATTAT 
CATGCAGAGA AGACCGTCGC TCTAGATTTT ACTGTCCCTC GTGCGCCAGT CAGTTCTGTT 
GCCAATAACG TTGCTTCAAG TAACAGTCAG CCGGTTGCTG GAGGGCCGCT CTTTCCTAGG 
CCGTCTGTCA GCTATCAGAA TGGTCAGGCA GGCCTAGATG TTAACAAGCA GGGTTTTTAT 
CAGCAGGTCA CTAAGGATGG GCCCCCGCAG CAGCAGCCTG TTATGGATAT CCAACACCAG 
GGAGGAGTGG GCCTGGGGGA ATACATGGTC CCATCCTCTC AGCCACTGTC CTTGGTTCTG 
CCTGCACAAG CGCCTCCAGT GGGTAAATCG GTTCCATACC CTCCACAAGC TGAAAGCCAA 
GTTCAAACCT TGCAGAACCC TAGTGGAACT CCTTATAACA GTGCGCCAGA ACCTGCACCT 
AAATACGGAC CAACTGAACA GCCTTCATAT CCAGCTCCGC CTGTTCCTTC CTCATCAGCC 
ACAGTGAACC ACATAGCAAC ATATCAAGAG CAGCCCAGGC CTCCTGCCTC CCATACCCCT 
CAGCAAGCTG ATCCTTATTA CTACAGGTCT TACGGTGCCT ACCCACAGCC TTACCAACCC 
TTGGACCCAA GGGCAGCACC TCTTTACTAT CCGGACCCTT ATGGCGCTTA TGACCCCACC 
TACAGGCAGT ACAGTGGGTC TGCATATGTG AACCCTGGCA ATTACTCCTA TCAGGAGCCC 
GAACGTCCCA GTTCCAGATC CAGCCACTGC TCCGATCGCC CCTCCTCAAG GCAGGGCTAT 
AGCAGTAAAG GAGACTGGAA TGCACACAGT GGTTACTACT CAGGGTATTA TCAGAGTCAG 
TATGATTATG CAGACCCCAG CCGCTGGGAC CGATATCCTG GAGCCTATGA CCCTGGCTAT 
AGAGACCCCA GCAGTAATTA CTGGAATTAT ATGTACAACA GCCGTGATGA TGTGTACCAA 
AGGAACGTGC CTACTGCTTA TACCACCAGA TATGACGTTT ATGAAGACCG CTGGCAATAT 
GACCCACGTT ATGTGGGAGG CTTTGATGAA GAGCCACAGA GAGACCTCAC GAAGGACGAC 
TTTGACCGAC GTAGTGTTCA CAGTGAGCAT TCGGTCGATA GCATGCACAG CGGCCGGAGT 
GCACACAGTC GGAGAAGCAG TTTCAGCTCC CATTCACAAC ATAGCCAGAT CTACAAAAGC 
CAGCCAGACC TGTCTGCCAA CGCGTATGAA CCCCAAATAC AGAATCCTCC TCTCAATGAG 
TATTCGTATG GCGTGTACCC CAATGACTAC GGCACCCAGC AGGCGCTTGA TGATTACTCT 
TACGGGTATC CCAAGGGCAC AGAGTGGCAA CCTGTAGAAC AAGTGCCTTC AAGGCCGTTG 
ACTCCAGAGA AGTTTGGGAG CCCACACATC TGTGCCAGGT TTGGTTCTGG GGGTCAGTTA 
ATAAAGGTGC TGCCTAACCT TCCTTCAGAC GGACAGCCGG CCTTAGTGGA AATCCACAAC 
ATGGAGGTCA TTATGCAGAA CAGCGCAGAG CAGGAACAGC TGAGAAGTTT CCCTGGACCT 
TTAGTGAAGG ATGAAACTCA TAAAGTTGAC GTCATCAGTT TTGCCCAGAA GAAATTCAAG 
GAGTGTTCTC AGAATGAGAG CCTGTTAGAC CAAGAGTCTG CTTGCCTGCT CTGGGACTTC 
ATTGTGCTTC TGTGCCGGCA AAATGGGACT GTTGTTGGAA CAGATATCGC TGAGCTGCTT 
TTGAGAGATC ACAAGACAGT GTGGCTTCCT GCAAAGTCTC CCAACGAAGC CAACCTGATC 
GATTTCAGCA ATGAGCCGCT CGAGCAGCAG GAGGAAGAGT CAGGGGCTTC TCAGTTGTCC 
TTCCTCACGG ATTCTTTGCA AAGCACCGGG GAAGCTCTAG AGAAGGAGAC TGAGCGGTTC 
AGAGAGCTTC TACTTTTTGG GCGTAAAAAG GATGCCTTAG AATCTGCTAT GAAAAATGGA 
CTGTGGGGCC ACGCTTTGCT CCTAGCTAGC AAAATGGACA GCCGAACGCA CGCCAGAGTT 
ATGACAAGGT TTGCCAACAG CCTTCCAATT AACGATCCCC TGCAGACTGT GTATCAGCTC 
TTGTCTGGGA GAATGCCAGC AGCAGCCACG TGCTGCGGAG ATGAGAAATG GGGTGACTGG 
CGCCCTCACC TGGCGATGGT GCTGTCTAAT CTGACCAACA ATGTTGACGT TCCCACCAGG 
GCCATGACAA CAATGGGAGA TACATTAGCA TCCCGGGGGC TGATGGATGC CTCACACTTC 
TGCTATTTAA TGGCACAAGT CGGCTTTGGC ATTTTCACCA AGAAATCTAC TAAGTTGGTC 
CTCATTGGAT CCAACCACAG TTTATCATTT GCAAAGTTTT GCACTAACGA AGCCATCCAG 
AGAACAGAGG CTTATGAATA TGCACAGGCA CTTGGCACCC AGTCTTTCTC TCTCCCGAAT 
ATACAGGTTT ACAAGTTCAT CTACGCTTGC CGTTTGGCGG AACACGGATT ATCTGCACAA 
GCTTTCCATT ACTGTGAAGT CATCTCCAAG ACCATCTTGA AGCACCCGTC TTGCTACTCC 
CCTGTGCTTG TCAGCCAGCT ACTGGAGGTG TCCTCCCACT TGAGGTTCTT TGACCCACAG 
CTGAAGGAGA AGCCAGAACA GGAGTTATTT ATCGACCCTC CTTGGCTGCA GCATCTCCGC 
ACCTTGGATA AGCAAATGAA GCATGGTACT GTAGTTTATG ACATGGGCAG AACCACACCT 
CAGCAGTTTG CCTGCAGTAC ACCAAGCTCG GAGATAGACC ATGTGAGCCA ATCAGATGGT 
TTTGGAATAG CCGGGGATAT ACCAACTCAT ACAGAGAATC CATTACTGGC CAATCTCCTG 
CCCAACATGA TGCCTCCAGT ACAAGCTGTG CTAGTTCCTG CTGGTCCTGA AAATCCGCCA 
CCTGTATATC CACCCTACAC AGGGAATGAT CCCTCCTACC CAGGTACACC TCAGGGTCTA 
GCTCCCTCAT CTGGGCCTCC AACTCCATCT ACTGCTCCAA ATTTTGTGCC GTTTGGTGTG 
GAACAGGCAA CTGTTTATCC ACCTGCTGCT CTACAGCCAT ACCCACCTCT TCCCCAGGCA 
ACAGATTGTG CTCCTCCAAT GCAGATGAAT CAAGATCCAG GACTGCAAAG GATTCAGCAG 
AATTCCCCTA CCAAAACCAC CTTCGCTGAT CCTGAAGATT TCTACGGTGT CATGGCAAAA 
ATGGTTCCCG GGCAGAGATC ACGGACAGTG TCACAGTCCT CATCACACAT GAGGCGGACA 
CGGACAACCT CAGAGTCATC AACCCATTCT ATTGGGTCTG GACGTAGAAG TTCCATGGGC 
ATCCAACCAT CGCCTCCTCC TGCCTCTATC CCAGAGGCAG ACAGACCAGA AGAGAAGAAC 
AAACATAAGT CTCATGGTCC ATCCCAAGGG ATTGTGTGGG ATGAGAAGCA GCAGCGGTGG 
ACTAACCTGG ATGAACCAGA AGAAGAGAGA AAACCGTTGC CTCCACCACC ACCAGGGATG 
CTGAAAAAAC CACAGACTGC TCCATCTGTC CCAGGTGGTC CTCTCAACCC TTCTGTCAAT 
GTTTTCTCCA TCAGAGCAGC AGGGGCAAGA GGTCGTTACA TTGATGTCTT AAACCCAGGG 
GGTACCCGGC CCACTAACTC GGTCCCTCCG CCTGCTGATT TGTTTGCACC TTTGGCCCCC 
ATGCCTATCC CCACGAACCT GTTTGTTCCA AATGCAGTCC CAGAAGAGGG TCAGCCTGCG 
GAAGCCAGTG GGGCAGAAAT GGTGCAACCT GCGGGTCAGA CGGGGCTCAG TAACTTTTCC 
CAGGCACAGT TACCCGGTTT GCCCCCTGTG TCTGATCTTC CAACAAATCA GGAAGATGGG 
CAGATGGGAG AGCTTTCCCG ATCTAGCTCT CTCAGTTCCT TATCCCGTGA AGTAAGCCAG 
CATATGGAGC AGGCTCACGT GGCCCCTGCG TCAGGCCCCC CACCTACAGG GGCTGTCCCC 
TTCTACAACC CCTCCCAGTT TTCACAACAG CAGCCTCCTT CTGCCCAAGC AGGTGGGCTA 
CGACCTGGAC GTCTCGGCCA GAGGAAATAT CCTTCCTTAA AATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_018088071.1
CDS	215..7219
Translation	MQPPPQARHPGTGGPPRMGLGNNAHWRKRGPNPVPVASASVQPVTDPFAFGRITPQSMAQGNVPIGNPLMAPSVSPSFYPQPVPVHASAAVDQGHGSPASLPGFVPQPAATGGMYTNVSVPSPSSAPFHNYPAATSSAQPGLSNIQTFQNTASGLENAFLPVHIRAQSTQGINYNPPSSSPLQHFIPAPSQPTNAPSGSLQSVPPASAQNFPLPVQQPSHNAPYYSPPYLEVSNLVPQANETVAQQRSAPGHYPPATVANPTFQHSTSQPTILNPIDYMHRHNPDQNWVKQPHKDQQHPPFPSPNPVQYPTQPPEPHQEVTNASGHVSTNVDSGTISMFFKGDEEENVEIVSSDQQNASVKVDHGSSHYQNSGHEGVPSLLPQPTVSSMSSLTPISVSQNVGNLTQPHSDWRQDVFPVGDKISKPKVGSELPYDNVENIECVQNQEVPPNEPNHFNLYPSNVNSDTYRYGSMPGLSLQKNVSSQPEAGPNLETPVTVGCPVRSDSVSSNYSNLSHKSLPVPTRPQELAGTFIQKERGRLEGDSSHGFFKQIDSLPMAGDAVGQNMGQHTFHSNLSQPPTPSPPKPTGIFMTSANSSFEPVRSHSIGLKASDVDQLKMGGDLREGHNVPPSASLPAASPGNLEQPPDNLESIFLGKRNSHGNITSNGNLFHSAGDPPLDNISLLLERRPSSRAHGSNKKCESPATTLWAQGELPNFGGNVLLAPAAPSLYVPPKPQTEIIQPPEDGSYDSCSGGTDMGKFQTSLEEQISSENLENPPKITDEEQLPSQASSGYASLLSSPPTEVLQNQPLLIAQPNKTYNLSQPNNFSLSLPNKVKNINSLKELGAADKPGPQSLPPAVGNFNSNDNTVFSLIQVPPEASNPPNYKSAATNDKFSESLHNLSSILQRQSSANQMPLNLAAESQKALKGENATVDLANKNPIPAALPTGPRFPANPNGKPNYHAEKTVALDFTVPRAPVSSVANNVASSNSQPVAGGPLFPRPSVSYQNGQAGLDVNKQGFYQQVTKDGPPQQQPVMDIQHQGGVGLGEYMVPSSQPLSLVLPAQAPPVGKSVPYPPQAESQVQTLQNPSGTPYNSAPEPAPKYGPTEQPSYPAPPVPSSSATVNHIATYQEQPRPPASHTPQQADPYYYRSYGAYPQPYQPLDPRAAPLYYPDPYGAYDPTYRQYSGSAYVNPGNYSYQEPERPSSRSSHCSDRPSSRQGYSSKGDWNAHSGYYSGYYQSQYDYADPSRWDRYPGAYDPGYRDPSSNYWNYMYNSRDDVYQRNVPTAYTTRYDVYEDRWQYDPRYVGGFDEEPQRDLTKDDFDRRSVHSEHSVDSMHSGRSAHSRRSSFSSHSQHSQIYKSQPDLSANAYEPQIQNPPLNEYSYGVYPNDYGTQQALDDYSYGYPKGTEWQPVEQVPSRPLTPEKFGSPHICARFGSGGQLIKVLPNLPSDGQPALVEIHNMEVIMQNSAEQEQLRSFPGPLVKDETHKVDVISFAQKKFKECSQNESLLDQESACLLWDFIVLLCRQNGTVVGTDIAELLLRDHKTVWLPAKSPNEANLIDFSNEPLEQQEEESGASQLSFLTDSLQSTGEALEKETERFRELLLFGRKKDALESAMKNGLWGHALLLASKMDSRTHARVMTRFANSLPINDPLQTVYQLLSGRMPAAATCCGDEKWGDWRPHLAMVLSNLTNNVDVPTRAMTTMGDTLASRGLMDASHFCYLMAQVGFGIFTKKSTKLVLIGSNHSLSFAKFCTNEAIQRTEAYEYAQALGTQSFSLPNIQVYKFIYACRLAEHGLSAQAFHYCEVISKTILKHPSCYSPVLVSQLLEVSSHLRFFDPQLKEKPEQELFIDPPWLQHLRTLDKQMKHGTVVYDMGRTTPQQFACSTPSSEIDHVSQSDGFGIAGDIPTHTENPLLANLLPNMMPPVQAVLVPAGPENPPPVYPPYTGNDPSYPGTPQGLAPSSGPPTPSTAPNFVPFGVEQATVYPPAALQPYPPLPQATDCAPPMQMNQDPGLQRIQQNSPTKTTFADPEDFYGVMAKMVPGQRSRTVSQSSSHMRRTRTTSESSTHSIGSGRRSSMGIQPSPPPASIPEADRPEEKNKHKSHGPSQGIVWDEKQQRWTNLDEPEEERKPLPPPPPGMLKKPQTAPSVPGGPLNPSVNVFSIRAAGARGRYIDVLNPGGTRPTNSVPPPADLFAPLAPMPIPTNLFVPNAVPEEGQPAEASGAEMVQPAGQTGLSNFSQAQLPGLPPVSDLPTNQEDGQMGELSRSSSLSSLSREVSQHMEQAHVAPASGPPPTGAVPFYNPSQFSQQQPPSAQAGGLRPGRLGQRKYPSLK

Target ORF information:

RefSeq Version	XM_018232582.1
Organism	Xenopus laevis(African clawed frog)
Definition	Xenopus laevis protein transport protein Sec16A (LOC100037049), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_018232582.1

ORF Insert Sequence:

ATGCAGCCCC CTCCGCAAGC AAGGCATCCT GGCACGGGTG GACCTCCTCG CATGGGACTT 
GGCAATAATG CCCACTGGCG CAAAAGGGGA CCTAATCCTG TACCAGTAGC TTCAGCTTCA 
GTGCAGCCAG TAACAGACCC TTTTGCCTTT GGCCGTATTA CCCCACAAAG TATGGCACAG 
GGCAATGTCC CGATAGGAAA CCCACTGATG GCACCCAGTG TTTCCCCTTC TTTTTACCCT 
CAACCCGTTC CTGTACATGC ATCGGCTGCT GTAGATCAAG GGCATGGGTC TCCTGCATCA 
CTACCAGGAT TTGTTCCACA ACCTGCTGCT ACGGGGGGGA TGTATACAAA TGTGTCTGTC 
CCATCACCAT CAAGTGCCCC CTTTCACAAC TACCCAGCCG CCACCTCTAG TGCTCAGCCT 
GGACTCAGTA ATATTCAGAC TTTTCAGAAT ACAGCAAGTG GACTTGAAAA TGCCTTTCTG 
CCAGTGCACA TCAGGGCTCA GTCAACACAA GGCATTAATT ATAACCCTCC ATCTTCATCA 
CCTTTGCAGC ACTTTATCCC TGCACCATCT CAGCCAACTA ATGCTCCCTC TGGGTCCTTA 
CAAAGTGTAC CTCCTGCCTC AGCTCAGAAC TTCCCCCTTC CTGTTCAACA GCCATCCCAT 
AATGCTCCTT ATTACTCCCC ACCTTATTTA GAAGTGTCTA ATCTGGTCCC TCAAGCCAAC 
GAAACAGTGG CACAGCAGAG GTCAGCTCCT GGGCATTACC CGCCTGCCAC TGTCGCCAAT 
CCTACATTTC AACACAGCAC TTCACAACCC ACCATTTTAA ATCCGATAGA CTATATGCAC 
CGCCACAATC CTGACCAGAA CTGGGTTAAG CAACCTCACA AGGATCAGCA GCATCCACCT 
TTTCCCAGTC CTAACCCCGT ACAATACCCA ACTCAGCCAC CTGAGCCCCA TCAGGAGGTG 
ACTAATGCAT CTGGTCATGT CTCCACCAAT GTCGACTCTG GTACTATATC TATGTTTTTT 
AAAGGGGATG AAGAAGAAAA TGTAGAAATA GTTTCTTCCG ACCAGCAGAA TGCATCTGTT 
AAAGTGGATC ATGGTTCTTC TCATTACCAG AACTCTGGAC ATGAAGGTGT GCCTTCTCTC 
CTGCCACAGC CCACAGTCAG CAGCATGTCG TCACTTACAC CAATCTCTGT GTCTCAGAAT 
GTTGGCAATT TGACCCAACC ACATTCAGAT TGGAGGCAGG ATGTGTTTCC TGTTGGCGAT 
AAAATTTCTA AACCCAAAGT TGGAAGTGAA TTGCCCTATG ACAATGTTGA AAACATAGAG 
TGTGTACAAA ACCAAGAGGT TCCCCCCAAC GAGCCCAATC ATTTTAACCT TTATCCTTCT 
AATGTAAATT CAGATACGTA CCGCTATGGG TCCATGCCTG GACTCTCCCT ACAAAAGAAT 
GTTAGCAGCC AACCTGAAGC TGGTCCGAAT CTAGAGACGC CTGTCACAGT GGGTTGTCCT 
GTCAGATCTG ATAGTGTTTC ATCAAATTAT AGCAACCTGA GTCACAAAAG CCTGCCTGTG 
CCCACCCGGC CTCAAGAGCT AGCCGGGACA TTTATTCAGA AGGAGAGAGG AAGGCTTGAA 
GGTGACTCCT CCCATGGTTT CTTTAAGCAA ATTGATTCTT TGCCCATGGC AGGTGATGCT 
GTAGGCCAGA ATATGGGTCA GCACACGTTC CACAGCAATT TATCCCAGCC ACCAACTCCA 
AGTCCTCCTA AACCTACTGG GATCTTCATG ACGAGTGCAA ATAGTTCTTT CGAACCTGTT 
AGATCGCACA GCATTGGTTT GAAAGCGTCC GACGTAGACC AATTGAAAAT GGGTGGAGAC 
TTAAGAGAGG GGCATAATGT TCCACCAAGC GCCTCTCTCC CAGCAGCTTC CCCTGGTAAC 
TTGGAACAAC CACCAGATAA CCTGGAATCC ATATTCTTAG GTAAAAGAAA TTCTCACGGA 
AACATTACCA GCAATGGGAA CTTGTTCCAT TCTGCTGGCG ACCCGCCATT GGATAATATA 
TCCTTACTGC TTGAGAGACG TCCATCGTCA AGAGCACACG GGTCTAACAA GAAATGCGAA 
AGCCCGGCAA CCACCCTGTG GGCTCAAGGT GAGCTTCCAA ATTTTGGCGG AAATGTTTTG 
TTGGCTCCTG CAGCTCCTTC ACTCTATGTG CCCCCTAAAC CCCAAACCGA AATAATTCAG 
CCACCTGAAG ATGGGTCTTA TGATTCATGC TCGGGTGGAA CAGATATGGG GAAATTTCAG 
ACCTCTCTGG AAGAACAAAT TTCTTCGGAA AACCTTGAGA ATCCTCCAAA GATCACTGAT 
GAGGAGCAAC TTCCCTCGCA GGCAAGTTCA GGTTACGCCA GTTTGTTGTC TTCGCCGCCC 
ACTGAGGTAC TTCAGAATCA GCCCTTGCTA ATTGCCCAAC CAAATAAAAC CTATAATTTG 
TCACAGCCTA ATAATTTTTC ATTGTCGTTA CCAAATAAGG TAAAAAATAT TAATTCGCTT 
AAGGAACTGG GAGCAGCTGA CAAGCCGGGT CCTCAGAGTT TGCCTCCAGC TGTGGGGAAT 
TTTAACTCCA ATGACAATAC TGTGTTTTCC CTAATTCAAG TCCCACCCGA GGCTTCTAAT 
CCACCTAATT ACAAATCTGC TGCTACTAAT GATAAATTCT CTGAATCTCT TCACAATCTG 
AGTTCCATTT TGCAGCGGCA ATCATCAGCA AATCAAATGC CCCTCAATTT AGCTGCGGAA 
AGTCAAAAGG CACTTAAGGG TGAAAACGCG ACTGTCGATC TTGCAAATAA AAACCCGATT 
CCAGCTGCCC TTCCGACGGG GCCACGTTTC CCTGCCAATC CAAACGGCAA GCCAAATTAT 
CATGCAGAGA AGACCGTCGC TCTAGATTTT ACTGTCCCTC GTGCGCCAGT CAGTTCTGTT 
GCCAATAACG TTGCTTCAAG TAACAGTCAG CCGGTTGCTG GAGGGCCGCT CTTTCCTAGG 
CCGTCTGTCA GCTATCAGAA TGGTCAGGCA GGCCTAGATG TTAACAAGCA GGGTTTTTAT 
CAGCAGGTCA CTAAGGATGG GCCCCCGCAG CAGCAGCCTG TTATGGATAT CCAACACCAG 
GGAGGAGTGG GCCTGGGGGA ATACATGGTC CCATCCTCTC AGCCACTGTC CTTGGTTCTG 
CCTGCACAAG CGCCTCCAGT GGGTAAATCG GTTCCATACC CTCCACAAGC TGAAAGCCAA 
GTTCAAACCT TGCAGAACCC TAGTGGAACT CCTTATAACA GTGCGCCAGA ACCTGCACCT 
AAATACGGAC CAACTGAACA GCCTTCATAT CCAGCTCCGC CTGTTCCTTC CTCATCAGCC 
ACAGTGAACC ACATAGCAAC ATATCAAGAG CAGCCCAGGC CTCCTGCCTC CCATACCCCT 
CAGCAAGCTG ATCCTTATTA CTACAGGTCT TACGGTGCCT ACCCACAGCC TTACCAACCC 
TTGGACCCAA GGGCAGCACC TCTTTACTAT CCGGACCCTT ATGGCGCTTA TGACCCCACC 
TACAGGCAGT ACAGTGGGTC TGCATATGTG AACCCTGGCA ATTACTCCTA TCAGGAGCCC 
GAACGTCCCA GTTCCAGATC CAGCCACTGC TCCGATCGCC CCTCCTCAAG GCAGGGCTAT 
AGCAGTAAAG GAGACTGGAA TGCACACAGT GGTTACTACT CAGGGTATTA TCAGAGTCAG 
TATGATTATG CAGACCCCAG CCGCTGGGAC CGATATCCTG GAGCCTATGA CCCTGGCTAT 
AGAGACCCCA GCAGTAATTA CTGGAATTAT ATGTACAACA GCCGTGATGA TGTGTACCAA 
AGGAACGTGC CTACTGCTTA TACCACCAGA TATGACGTTT ATGAAGACCG CTGGCAATAT 
GACCCACGTT ATGTGGGAGG CTTTGATGAA GAGCCACAGA GAGACCTCAC GAAGGACGAC 
TTTGACCGAC GTAGTGTTCA CAGTGAGCAT TCGGTCGATA GCATGCACAG CGGCCGGAGT 
GCACACAGTC GGAGAAGCAG TTTCAGCTCC CATTCACAAC ATAGCCAGAT CTACAAAAGC 
CAGCCAGACC TGTCTGCCAA CGCGTATGAA CCCCAAATAC AGAATCCTCC TCTCAATGAG 
TATTCGTATG GCGTGTACCC CAATGACTAC GGCACCCAGC AGGCGCTTGA TGATTACTCT 
TACGGGTATC CCAAGGGCAC AGAGTGGCAA CCTGTAGAAC AAGTGCCTTC AAGGCCGTTG 
ACTCCAGAGA AGTTTGGGAG CCCACACATC TGTGCCAGGT TTGGTTCTGG GGGTCAGTTA 
ATAAAGGTGC TGCCTAACCT TCCTTCAGAC GGACAGCCGG CCTTAGTGGA AATCCACAAC 
ATGGAGGTCA TTATGCAGAA CAGCGCAGAG CAGGAACAGC TGAGAAGTTT CCCTGGACCT 
TTAGTGAAGG ATGAAACTCA TAAAGTTGAC GTCATCAGTT TTGCCCAGAA GAAATTCAAG 
GAGTGTTCTC AGAATGAGAG CCTGTTAGAC CAAGAGTCTG CTTGCCTGCT CTGGGACTTC 
ATTGTGCTTC TGTGCCGGCA AAATGGGACT GTTGTTGGAA CAGATATCGC TGAGCTGCTT 
TTGAGAGATC ACAAGACAGT GTGGCTTCCT GCAAAGTCTC CCAACGAAGC CAACCTGATC 
GATTTCAGCA ATGAGCCGCT CGAGCAGCAG GAGGAAGAGT CAGGGGCTTC TCAGTTGTCC 
TTCCTCACGG ATTCTTTGCA AAGCACCGGG GAAGCTCTAG AGAAGGAGAC TGAGCGGTTC 
AGAGAGCTTC TACTTTTTGG GCGTAAAAAG GATGCCTTAG AATCTGCTAT GAAAAATGGA 
CTGTGGGGCC ACGCTTTGCT CCTAGCTAGC AAAATGGACA GCCGAACGCA CGCCAGAGTT 
ATGACAAGGT TTGCCAACAG CCTTCCAATT AACGATCCCC TGCAGACTGT GTATCAGCTC 
TTGTCTGGGA GAATGCCAGC AGCAGCCACG TGCTGCGGAG ATGAGAAATG GGGTGACTGG 
CGCCCTCACC TGGCGATGGT GCTGTCTAAT CTGACCAACA ATGTTGACGT TCCCACCAGG 
GCCATGACAA CAATGGGAGA TACATTAGCA TCCCGGGGGC TGATGGATGC CTCACACTTC 
TGCTATTTAA TGGCACAAGT CGGCTTTGGC ATTTTCACCA AGAAATCTAC TAAGTTGGTC 
CTCATTGGAT CCAACCACAG TTTATCATTT GCAAAGTTTT GCACTAACGA AGCCATCCAG 
AGAACAGAGG CTTATGAATA TGCACAGGCA CTTGGCACCC AGTCTTTCTC TCTCCCGAAT 
ATACAGGTTT ACAAGTTCAT CTACGCTTGC CGTTTGGCGG AACACGGATT ATCTGCACAA 
GCTTTCCATT ACTGTGAAGT CATCTCCAAG ACCATCTTGA AGCACCCGTC TTGCTACTCC 
CCTGTGCTTG TCAGCCAGCT ACTGGAGGTG TCCTCCCACT TGAGGTTCTT TGACCCACAG 
CTGAAGGAGA AGCCAGAACA GGAGTTATTT ATCGACCCTC CTTGGCTGCA GCATCTCCGC 
ACCTTGGATA AGCAAATGAA GCATGGTACT GTAGTTTATG ACATGGGCAG AACCACACCT 
CAGCAGTTTG CCTGCAGTAC ACCAAGCTCG GAGATAGACC ATGTGAGCCA ATCAGATGGT 
TTTGGAATAG CCGGGGATAT ACCAACTCAT ACAGAGAATC CATTACTGGC CAATCTCCTG 
CCCAACATGA TGCCTCCAGT ACAAGCTGTG CTAGTTCCTG CTGGTCCTGA AAATCCGCCA 
CCTGTATATC CACCCTACAC AGGGAATGAT CCCTCCTACC CAGGTACACC TCAGGGTCTA 
GCTCCCTCAT CTGGGCCTCC AACTCCATCT ACTGCTCCAA ATTTTGTGCC GTTTGGTGTG 
GAACAGGCAA CTGTTTATCC ACCTGCTGCT CTACAGCCAT ACCCACCTCT TCCCCAGGCA 
ACAGATTGTG CTCCTCCAAT GCAGATGAAT CAAGATCCAG GACTGCAAAG GATTCAGCAG 
AATTCCCCTA CCAAAACCAC CTTCGCTGAT CCTGAAGATT TCTACGGTGT CATGGCAAAA 
ATGGTTCCCG GGCAGAGATC ACGGACAGTG TCACAGTCCT CATCACACAT GAGGCGGACA 
CGGACAACCT CAGAGTCATC AACCCATTCT ATTGGGTCTG GACGTAGAAG TTCCATGGGC 
ATCCAACCAT CGCCTCCTCC TGCCTCTATC CCAGAGGCAG ACAGACCAGA AGAGAAGAAC 
AAACATAAGT CTCATGGTCC ATCCCAAGGG ATTGTGTGGG ATGAGAAGCA GCAGCGGTGG 
ACTAACCTGG ATGAACCAGA AGAAGAGAGA AAACCGTTGC CTCCACCACC ACCAGGGATG 
CTGAAAAAAC CACAGACTGC TCCATCTGTC CCAGGTGGTC CTCTCAACCC TTCTGTCAAT 
GTTTTCTCCA TCAGAGCAGC AGGGGCAAGA GGTCGTTACA TTGATGTCTT AAACCCAGGG 
GGTACCCGGC CCACTAACTC GGTCCCTCCG CCTGCTGATT TGTTTGCACC TTTGGCCCCC 
ATGCCTATCC CCACGAACCT GTTTGTTCCA AATGCAGTCC CAGAAGAGGG TCAGCCTGCG 
GAAGCCAGTG GGGCAGAAAT GGTGCAACCT GCGGGTCAGA CGGGGCTCAG TAACTTTTCC 
CAGGCACAGT TACCCGGTTT GCCCCCTGTG TCTGATCTTC CAACAAATCA GGAAGATGGG 
CAGATGGGAG AGCTTTCCCG ATCTAGCTCT CTCAGTTCCT TATCCCGTGA AGTAAGCCAG 
CATATGGAGC AGGCTCACGT GGCCCCTGCG TCAGGCCCCC CACCTACAGG GGCTGTCCCC 
TTCTACAACC CCTCCCAGTT TTCACAACAG CAGCCTCCTT CTGCCCAAGC AGGTGGGCTA 
CGACCTGGAC GTCTCGGCCA GAGGAAATAT CCTTCCTTAA AATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

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LOC100037049 cDNA ORF clone, Xenopus laevis(African clawed frog)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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