LOC104877973 cDNA ORF clone, Vitis vinifera(wine grape)

Gene
Clones
gRNAs

Summary

Gene Symbol	LOC104877973
Entrez Gene ID	104877973
Full Name	TMV resistance protein N-like
Gene Type	protein-coding
Organism	Vitis vinifera(wine grape)
Genome	Chromosome: 18 Map Location: 18\|18

mRNA and Protein(s)

mRNA	Protein	Name
XM_010647605.2	XP_010645907.2	TMV resistance protein N-like
XM_019217900.1	XP_019073445.1	TMV resistance protein N-like

Pathways from BioSystems
Gene Ontology

The following LOC104877973 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC104877973 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OVa39371	XM_019217900.1 Latest version!	Vitis vinifera TMV resistance protein N-like (LOC104877973), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OVa39371	XM_010647605.2 Latest version!	Vitis vinifera TMV resistance protein N-like (LOC104877973), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LOC104877973 ( XM_010647605.2 ) cDNA ORF clone, Vitis vinifera(wine grape) -> XP_010645907.2 Vitis vinifera TMV resistance protein N-like (LOC104877973), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OVa39371
Clone ID Related Accession (Same CDS sequence)	XM_010647605.2 , XM_019217900.1
Accession Version	XM_010647605.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3126bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1479657600000
Organism	Vitis vinifera(wine grape)
Product	TMV resistance protein N-like
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003724191.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Nov 22, 2016 this sequence version replaced XM_010647605.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Vitis vinifera Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTGCTG CTTTTTCTTC TTCTCAGAAG AGCTATGATG TCTTCCTCAG TTTCAGAGGA 
GACGACACCC GCAACAATTT CACTGCCCAT CTCCTTCAGG AGTTGCGCAC CAAAGGAATC 
AACACTTTCT TTGATGAGGA TAAGCTCGAG AAAGGTCGAG TCATATCCCC TGCACTCATT 
ACAGCTATTG AAAACTCCAT GTTTTCTATC ATTGTTTTGT CGGAAAACTA TGCATCTTCT 
AGGTGGTGCT TGGAGGAGAT GGTCAAGATA CTAGAATGCA ATAGATCCAA GGAGGAGAGG 
GTTCTACCAA TTTTCTACAA CGTGGATCCA TCAGATGTGC GGAATCACAT GGGAAAATTT 
GGAGAAGCCT TGGCTAAACA TGAAGAGAAT TTGGAGGAGA ACGGGGAGAG GGTGAAAATT 
TGGAGGGACG CTCTGACTGA AGTTGCAAAT TTATCTGGCT GGGATTCAAG GAACAAGAAT 
GAGCCTCTGC TAATAAAAGA AATCGTTATA AAACTTTTGA AGAAGTTGTT AAATACATGG 
ACAAGTGATA CTGAAGAGAA CCTGGTTGGA ATACAGTCCC GCATTCAAAA ACTGAGAATG 
CTATTGTGTT TACAATCAGA TGATGTTCGG ATGGTAGGAA TTTGTGGTAT GGGTGGAATA 
GGGAAAACAA CCCTTGCTAG AGCTATTTAT AGCCAAGTTT CTAACCAATT TGAAGCTTGC 
TCCTTTCTTG AAATTGCAAA TGATTTCAAA GAGCAGGATT TAACTAGTTT AGCAGAGAAA 
CTTCTTTCTC AATTATTGCA GGAAGAAAAT CTCAAAATAA AAGGATCCAC TTCTATAAAA 
GCAAGGCTCC ACTCAAGAAA GGTCCTTGTT GTTCTTGATA ATGTGAATAA TCTAACAATA 
TTGGAACATT TAGCTGGAAA TCAAGATTGG TTTGGTCAAG GGAGTAGAAT TATCGTAACT 
ACTCGGGATC AACGTTTGTT AATTCAACAT AAAGTTGATT ATTATGAGGT TGCAGAATTT 
AATGGTGATG AAGCATTTGA GTTCCTCAAA CATCATTCGT TAAAATATGA ACTTCTTGAA 
AATGATTTAC AGGAGCTTTC AAGAGAAATA ATATTTTATG CTAAAGGCCT TCCATTGGCT 
CTTAGAGTTT TAGGTTCTCT TTTATTTGGC ATGAACAAAG ACGAGTGGAG AGATTACTTG 
GTCAAACTAA AAAGTACTCC TAATATAGAA ATTCAGGAAG TACTTAGATT AAGTTACGAT 
AGGCTAGATG ATGAAGAGAA GAATATATTT TTGGATATTG CATGTTTCTT TAAAGGAGAG 
GACAAAGATC ATGTGGTGGA AATACTGAAG GGTTGTGGCT TCTCTGCAAA ATGTGGAATA 
AAAACTCTCA TTAACAAGTC TCTCATAACT ATTAATTTTG CTAACAAGTT GGAGATGCAT 
GACTTAATAC AAGAAATGGG TAAGGGTATT GTTCGCCAAG AATGTCCAAA AGAGCCTGAA 
AGACGAAGTA GATTGTGGGA ACATGAAGAT ATCTTTGATG TGTTGAAAAG AAATATGGGA 
AGTGAAAAAA TTGAAGGCAT ATTCCTCAAC TTGTCTCATT TAGAAGATAC ACTAGACTTC 
ACCATTGAAG CCTTTGCTGG GATGAAAAAA CTTAGATTGC TCAAAGTCTA TAATTCTAAG 
AGTATTTCAA GAGACTTCAG AGATACCTTT AACAACAAGG TGAATTGTAG AGTGCGGTTT 
GCCCATGAAT TTAAGTTTTG TTCCAATGAT TTGAGGTACT TATATTGGCA TGGATACTCT 
TTGAAATCAT TACCAAAGGA TTTCAGCCCA AAGCATCTTG TTGAGCTCAG TATGCCTTAT 
AGTCACATTA AAAAACTTTG GAAAGGAATC AAGGTTCTTG AAAGGTTAAA ATCCATAGAT 
CTCAGCCACT CCAAGTACTT AATACAAACT CCAGATTTCT CAGGGATCAC CAACCTTGAA 
CGTCTAGTTT TAGAAGGTTG TATAAATTTG CCTAAGGTTC ACCCATCACT TGGAGTTTTG 
AAGAAGCTAA ATTTCTTGAG TTTAAAAAAT TGCACAATGC TTAGAAGGCT TCCAAGCAGC 
ACTTGTAGTT TGAAATCTCT TGAAACCTTT ATTCTTTCTG GTTGCTCCAA ATTTGAAGAA 
TTTCCAGAGA ATTTTGGGAA CTTAGAAATG TTGAAGGAGC TTCACGCAGA TGGAATTGTG 
GTAAGAGTAC TACCCCCCTC CTTTTTTTCC ATGAGAAACC TTGAAAAGTT ATCTTTCGGT 
GGATGTAAAG GACCAGCATC TGCCTCATGG TTGTGGCCAA AAAGAAGTTC AAATTCTATT 
TGTTTCACGG TACCTTCTTC CTCAAACTTA TGCTCTTTAA AGAACCTAGA TTTAAGTTAC 
TGCAATATAT CAGATGGAGC AAATGTTAGT GGCCTTGGCT TCTTAGTTTC ACTTGAATGG 
TTAAATTTAA GTGGAAACAA CTTTGTCACT TTGCCTAACA TGAGTGGACT CTCTCACCTG 
GAAACGCTTC GGTTGGGAAA TTGTAAAAGA CTAGAGGCAC TTTCACAGCT TCCATCAAGC 
ATACGTTCTT TAAATGCAAA AAATTGCACA TCGTTGGGAA CAACTGAATT ACTCAACTTG 
CTACTTACAA CGAAGGTGCA TACTCTGCTG AGACCGCGGG AACTCGAATC CCTAGATTCT 
ACATTCGGTG TTGTGATTCC TGGGAGTAGA ATACCAGATT GGATCAGGTA TCAGAGCTCG 
AGGAATGTAA TTGAAGCAGA CCTACCCCTA AATTGGTCTA CCAACTGCCT GGGTTTTGCA 
TTGGCTCTTG TCTTCGGTGG CCGGTTTCCT GTCGCTTATG ATGATTGGTT TTGGGCACGT 
GTTTTCTTGG ACTTTGGCAC ATGTCGCCGT TCTTTCGAAA CTGGTATTTC TTTCCCCATG 
GAAAATAGTG TTTTCGCTGA AGGGGATCAT GTGGTTCTGA CTTTTGCACC AGTTCAACCC 
TCACTCAGTC CGCATCAAGT CATTCACATC AAGGCTACAT TTGCAATAAT GTCCGTGCCG 
AACTATTATG AAATTAAGAG ATGTGGGTTA GGTTTAATGT ATGTTAATGA AGAGGTCCAA 
AAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010645907.2
CDS	288..3413
Translation	MAAAFSSSQKSYDVFLSFRGDDTRNNFTAHLLQELRTKGINTFFDEDKLEKGRVISPALITAIENSMFSIIVLSENYASSRWCLEEMVKILECNRSKEERVLPIFYNVDPSDVRNHMGKFGEALAKHEENLEENGERVKIWRDALTEVANLSGWDSRNKNEPLLIKEIVIKLLKKLLNTWTSDTEENLVGIQSRIQKLRMLLCLQSDDVRMVGICGMGGIGKTTLARAIYSQVSNQFEACSFLEIANDFKEQDLTSLAEKLLSQLLQEENLKIKGSTSIKARLHSRKVLVVLDNVNNLTILEHLAGNQDWFGQGSRIIVTTRDQRLLIQHKVDYYEVAEFNGDEAFEFLKHHSLKYELLENDLQELSREIIFYAKGLPLALRVLGSLLFGMNKDEWRDYLVKLKSTPNIEIQEVLRLSYDRLDDEEKNIFLDIACFFKGEDKDHVVEILKGCGFSAKCGIKTLINKSLITINFANKLEMHDLIQEMGKGIVRQECPKEPERRSRLWEHEDIFDVLKRNMGSEKIEGIFLNLSHLEDTLDFTIEAFAGMKKLRLLKVYNSKSISRDFRDTFNNKVNCRVRFAHEFKFCSNDLRYLYWHGYSLKSLPKDFSPKHLVELSMPYSHIKKLWKGIKVLERLKSIDLSHSKYLIQTPDFSGITNLERLVLEGCINLPKVHPSLGVLKKLNFLSLKNCTMLRRLPSSTCSLKSLETFILSGCSKFEEFPENFGNLEMLKELHADGIVVRVLPPSFFSMRNLEKLSFGGCKGPASASWLWPKRSSNSICFTVPSSSNLCSLKNLDLSYCNISDGANVSGLGFLVSLEWLNLSGNNFVTLPNMSGLSHLETLRLGNCKRLEALSQLPSSIRSLNAKNCTSLGTTELLNLLLTTKVHTLLRPRELESLDSTFGVVIPGSRIPDWIRYQSSRNVIEADLPLNWSTNCLGFALALVFGGRFPVAYDDWFWARVFLDFGTCRRSFETGISFPMENSVFAEGDHVVLTFAPVQPSLSPHQVIHIKATFAIMSVPNYYEIKRCGLGLMYVNEEVQK

Target ORF information:

RefSeq Version	XM_010647605.2
Organism	Vitis vinifera(wine grape)
Definition	Vitis vinifera TMV resistance protein N-like (LOC104877973), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010647605.2

ORF Insert Sequence:

ATGGCTGCTG CTTTTTCTTC TTCTCAGAAG AGCTATGATG TCTTCCTCAG TTTCAGAGGA 
GACGACACCC GCAACAATTT CACTGCCCAT CTCCTTCAGG AGTTGCGCAC CAAAGGAATC 
AACACTTTCT TTGATGAGGA TAAGCTCGAG AAAGGTCGAG TCATATCCCC TGCACTCATT 
ACAGCTATTG AAAACTCCAT GTTTTCTATC ATTGTTTTGT CGGAAAACTA TGCATCTTCT 
AGGTGGTGCT TGGAGGAGAT GGTCAAGATA CTAGAATGCA ATAGATCCAA GGAGGAGAGG 
GTTCTACCAA TTTTCTACAA CGTGGATCCA TCAGATGTGC GGAATCACAT GGGAAAATTT 
GGAGAAGCCT TGGCTAAACA TGAAGAGAAT TTGGAGGAGA ACGGGGAGAG GGTGAAAATT 
TGGAGGGACG CTCTGACTGA AGTTGCAAAT TTATCTGGCT GGGATTCAAG GAACAAGAAT 
GAGCCTCTGC TAATAAAAGA AATCGTTATA AAACTTTTGA AGAAGTTGTT AAATACATGG 
ACAAGTGATA CTGAAGAGAA CCTGGTTGGA ATACAGTCCC GCATTCAAAA ACTGAGAATG 
CTATTGTGTT TACAATCAGA TGATGTTCGG ATGGTAGGAA TTTGTGGTAT GGGTGGAATA 
GGGAAAACAA CCCTTGCTAG AGCTATTTAT AGCCAAGTTT CTAACCAATT TGAAGCTTGC 
TCCTTTCTTG AAATTGCAAA TGATTTCAAA GAGCAGGATT TAACTAGTTT AGCAGAGAAA 
CTTCTTTCTC AATTATTGCA GGAAGAAAAT CTCAAAATAA AAGGATCCAC TTCTATAAAA 
GCAAGGCTCC ACTCAAGAAA GGTCCTTGTT GTTCTTGATA ATGTGAATAA TCTAACAATA 
TTGGAACATT TAGCTGGAAA TCAAGATTGG TTTGGTCAAG GGAGTAGAAT TATCGTAACT 
ACTCGGGATC AACGTTTGTT AATTCAACAT AAAGTTGATT ATTATGAGGT TGCAGAATTT 
AATGGTGATG AAGCATTTGA GTTCCTCAAA CATCATTCGT TAAAATATGA ACTTCTTGAA 
AATGATTTAC AGGAGCTTTC AAGAGAAATA ATATTTTATG CTAAAGGCCT TCCATTGGCT 
CTTAGAGTTT TAGGTTCTCT TTTATTTGGC ATGAACAAAG ACGAGTGGAG AGATTACTTG 
GTCAAACTAA AAAGTACTCC TAATATAGAA ATTCAGGAAG TACTTAGATT AAGTTACGAT 
AGGCTAGATG ATGAAGAGAA GAATATATTT TTGGATATTG CATGTTTCTT TAAAGGAGAG 
GACAAAGATC ATGTGGTGGA AATACTGAAG GGTTGTGGCT TCTCTGCAAA ATGTGGAATA 
AAAACTCTCA TTAACAAGTC TCTCATAACT ATTAATTTTG CTAACAAGTT GGAGATGCAT 
GACTTAATAC AAGAAATGGG TAAGGGTATT GTTCGCCAAG AATGTCCAAA AGAGCCTGAA 
AGACGAAGTA GATTGTGGGA ACATGAAGAT ATCTTTGATG TGTTGAAAAG AAATATGGGA 
AGTGAAAAAA TTGAAGGCAT ATTCCTCAAC TTGTCTCATT TAGAAGATAC ACTAGACTTC 
ACCATTGAAG CCTTTGCTGG GATGAAAAAA CTTAGATTGC TCAAAGTCTA TAATTCTAAG 
AGTATTTCAA GAGACTTCAG AGATACCTTT AACAACAAGG TGAATTGTAG AGTGCGGTTT 
GCCCATGAAT TTAAGTTTTG TTCCAATGAT TTGAGGTACT TATATTGGCA TGGATACTCT 
TTGAAATCAT TACCAAAGGA TTTCAGCCCA AAGCATCTTG TTGAGCTCAG TATGCCTTAT 
AGTCACATTA AAAAACTTTG GAAAGGAATC AAGGTTCTTG AAAGGTTAAA ATCCATAGAT 
CTCAGCCACT CCAAGTACTT AATACAAACT CCAGATTTCT CAGGGATCAC CAACCTTGAA 
CGTCTAGTTT TAGAAGGTTG TATAAATTTG CCTAAGGTTC ACCCATCACT TGGAGTTTTG 
AAGAAGCTAA ATTTCTTGAG TTTAAAAAAT TGCACAATGC TTAGAAGGCT TCCAAGCAGC 
ACTTGTAGTT TGAAATCTCT TGAAACCTTT ATTCTTTCTG GTTGCTCCAA ATTTGAAGAA 
TTTCCAGAGA ATTTTGGGAA CTTAGAAATG TTGAAGGAGC TTCACGCAGA TGGAATTGTG 
GTAAGAGTAC TACCCCCCTC CTTTTTTTCC ATGAGAAACC TTGAAAAGTT ATCTTTCGGT 
GGATGTAAAG GACCAGCATC TGCCTCATGG TTGTGGCCAA AAAGAAGTTC AAATTCTATT 
TGTTTCACGG TACCTTCTTC CTCAAACTTA TGCTCTTTAA AGAACCTAGA TTTAAGTTAC 
TGCAATATAT CAGATGGAGC AAATGTTAGT GGCCTTGGCT TCTTAGTTTC ACTTGAATGG 
TTAAATTTAA GTGGAAACAA CTTTGTCACT TTGCCTAACA TGAGTGGACT CTCTCACCTG 
GAAACGCTTC GGTTGGGAAA TTGTAAAAGA CTAGAGGCAC TTTCACAGCT TCCATCAAGC 
ATACGTTCTT TAAATGCAAA AAATTGCACA TCGTTGGGAA CAACTGAATT ACTCAACTTG 
CTACTTACAA CGAAGGTGCA TACTCTGCTG AGACCGCGGG AACTCGAATC CCTAGATTCT 
ACATTCGGTG TTGTGATTCC TGGGAGTAGA ATACCAGATT GGATCAGGTA TCAGAGCTCG 
AGGAATGTAA TTGAAGCAGA CCTACCCCTA AATTGGTCTA CCAACTGCCT GGGTTTTGCA 
TTGGCTCTTG TCTTCGGTGG CCGGTTTCCT GTCGCTTATG ATGATTGGTT TTGGGCACGT 
GTTTTCTTGG ACTTTGGCAC ATGTCGCCGT TCTTTCGAAA CTGGTATTTC TTTCCCCATG 
GAAAATAGTG TTTTCGCTGA AGGGGATCAT GTGGTTCTGA CTTTTGCACC AGTTCAACCC 
TCACTCAGTC CGCATCAAGT CATTCACATC AAGGCTACAT TTGCAATAAT GTCCGTGCCG 
AACTATTATG AAATTAAGAG ATGTGGGTTA GGTTTAATGT ATGTTAATGA AGAGGTCCAA 
AAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC104877973 ( XM_019217900.1 ) cDNA ORF clone, Vitis vinifera(wine grape) -> XP_019073445.1 Vitis vinifera TMV resistance protein N-like (LOC104877973), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OVa39371
Clone ID Related Accession (Same CDS sequence)	XM_010647605.2 , XM_019217900.1
Accession Version	XM_019217900.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3126bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1479657600000
Organism	Vitis vinifera(wine grape)
Product	TMV resistance protein N-like
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003724191.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Vitis vinifera Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTGCTG CTTTTTCTTC TTCTCAGAAG AGCTATGATG TCTTCCTCAG TTTCAGAGGA 
GACGACACCC GCAACAATTT CACTGCCCAT CTCCTTCAGG AGTTGCGCAC CAAAGGAATC 
AACACTTTCT TTGATGAGGA TAAGCTCGAG AAAGGTCGAG TCATATCCCC TGCACTCATT 
ACAGCTATTG AAAACTCCAT GTTTTCTATC ATTGTTTTGT CGGAAAACTA TGCATCTTCT 
AGGTGGTGCT TGGAGGAGAT GGTCAAGATA CTAGAATGCA ATAGATCCAA GGAGGAGAGG 
GTTCTACCAA TTTTCTACAA CGTGGATCCA TCAGATGTGC GGAATCACAT GGGAAAATTT 
GGAGAAGCCT TGGCTAAACA TGAAGAGAAT TTGGAGGAGA ACGGGGAGAG GGTGAAAATT 
TGGAGGGACG CTCTGACTGA AGTTGCAAAT TTATCTGGCT GGGATTCAAG GAACAAGAAT 
GAGCCTCTGC TAATAAAAGA AATCGTTATA AAACTTTTGA AGAAGTTGTT AAATACATGG 
ACAAGTGATA CTGAAGAGAA CCTGGTTGGA ATACAGTCCC GCATTCAAAA ACTGAGAATG 
CTATTGTGTT TACAATCAGA TGATGTTCGG ATGGTAGGAA TTTGTGGTAT GGGTGGAATA 
GGGAAAACAA CCCTTGCTAG AGCTATTTAT AGCCAAGTTT CTAACCAATT TGAAGCTTGC 
TCCTTTCTTG AAATTGCAAA TGATTTCAAA GAGCAGGATT TAACTAGTTT AGCAGAGAAA 
CTTCTTTCTC AATTATTGCA GGAAGAAAAT CTCAAAATAA AAGGATCCAC TTCTATAAAA 
GCAAGGCTCC ACTCAAGAAA GGTCCTTGTT GTTCTTGATA ATGTGAATAA TCTAACAATA 
TTGGAACATT TAGCTGGAAA TCAAGATTGG TTTGGTCAAG GGAGTAGAAT TATCGTAACT 
ACTCGGGATC AACGTTTGTT AATTCAACAT AAAGTTGATT ATTATGAGGT TGCAGAATTT 
AATGGTGATG AAGCATTTGA GTTCCTCAAA CATCATTCGT TAAAATATGA ACTTCTTGAA 
AATGATTTAC AGGAGCTTTC AAGAGAAATA ATATTTTATG CTAAAGGCCT TCCATTGGCT 
CTTAGAGTTT TAGGTTCTCT TTTATTTGGC ATGAACAAAG ACGAGTGGAG AGATTACTTG 
GTCAAACTAA AAAGTACTCC TAATATAGAA ATTCAGGAAG TACTTAGATT AAGTTACGAT 
AGGCTAGATG ATGAAGAGAA GAATATATTT TTGGATATTG CATGTTTCTT TAAAGGAGAG 
GACAAAGATC ATGTGGTGGA AATACTGAAG GGTTGTGGCT TCTCTGCAAA ATGTGGAATA 
AAAACTCTCA TTAACAAGTC TCTCATAACT ATTAATTTTG CTAACAAGTT GGAGATGCAT 
GACTTAATAC AAGAAATGGG TAAGGGTATT GTTCGCCAAG AATGTCCAAA AGAGCCTGAA 
AGACGAAGTA GATTGTGGGA ACATGAAGAT ATCTTTGATG TGTTGAAAAG AAATATGGGA 
AGTGAAAAAA TTGAAGGCAT ATTCCTCAAC TTGTCTCATT TAGAAGATAC ACTAGACTTC 
ACCATTGAAG CCTTTGCTGG GATGAAAAAA CTTAGATTGC TCAAAGTCTA TAATTCTAAG 
AGTATTTCAA GAGACTTCAG AGATACCTTT AACAACAAGG TGAATTGTAG AGTGCGGTTT 
GCCCATGAAT TTAAGTTTTG TTCCAATGAT TTGAGGTACT TATATTGGCA TGGATACTCT 
TTGAAATCAT TACCAAAGGA TTTCAGCCCA AAGCATCTTG TTGAGCTCAG TATGCCTTAT 
AGTCACATTA AAAAACTTTG GAAAGGAATC AAGGTTCTTG AAAGGTTAAA ATCCATAGAT 
CTCAGCCACT CCAAGTACTT AATACAAACT CCAGATTTCT CAGGGATCAC CAACCTTGAA 
CGTCTAGTTT TAGAAGGTTG TATAAATTTG CCTAAGGTTC ACCCATCACT TGGAGTTTTG 
AAGAAGCTAA ATTTCTTGAG TTTAAAAAAT TGCACAATGC TTAGAAGGCT TCCAAGCAGC 
ACTTGTAGTT TGAAATCTCT TGAAACCTTT ATTCTTTCTG GTTGCTCCAA ATTTGAAGAA 
TTTCCAGAGA ATTTTGGGAA CTTAGAAATG TTGAAGGAGC TTCACGCAGA TGGAATTGTG 
GTAAGAGTAC TACCCCCCTC CTTTTTTTCC ATGAGAAACC TTGAAAAGTT ATCTTTCGGT 
GGATGTAAAG GACCAGCATC TGCCTCATGG TTGTGGCCAA AAAGAAGTTC AAATTCTATT 
TGTTTCACGG TACCTTCTTC CTCAAACTTA TGCTCTTTAA AGAACCTAGA TTTAAGTTAC 
TGCAATATAT CAGATGGAGC AAATGTTAGT GGCCTTGGCT TCTTAGTTTC ACTTGAATGG 
TTAAATTTAA GTGGAAACAA CTTTGTCACT TTGCCTAACA TGAGTGGACT CTCTCACCTG 
GAAACGCTTC GGTTGGGAAA TTGTAAAAGA CTAGAGGCAC TTTCACAGCT TCCATCAAGC 
ATACGTTCTT TAAATGCAAA AAATTGCACA TCGTTGGGAA CAACTGAATT ACTCAACTTG 
CTACTTACAA CGAAGGTGCA TACTCTGCTG AGACCGCGGG AACTCGAATC CCTAGATTCT 
ACATTCGGTG TTGTGATTCC TGGGAGTAGA ATACCAGATT GGATCAGGTA TCAGAGCTCG 
AGGAATGTAA TTGAAGCAGA CCTACCCCTA AATTGGTCTA CCAACTGCCT GGGTTTTGCA 
TTGGCTCTTG TCTTCGGTGG CCGGTTTCCT GTCGCTTATG ATGATTGGTT TTGGGCACGT 
GTTTTCTTGG ACTTTGGCAC ATGTCGCCGT TCTTTCGAAA CTGGTATTTC TTTCCCCATG 
GAAAATAGTG TTTTCGCTGA AGGGGATCAT GTGGTTCTGA CTTTTGCACC AGTTCAACCC 
TCACTCAGTC CGCATCAAGT CATTCACATC AAGGCTACAT TTGCAATAAT GTCCGTGCCG 
AACTATTATG AAATTAAGAG ATGTGGGTTA GGTTTAATGT ATGTTAATGA AGAGGTCCAA 
AAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_019073445.1
CDS	288..3413
Translation	MAAAFSSSQKSYDVFLSFRGDDTRNNFTAHLLQELRTKGINTFFDEDKLEKGRVISPALITAIENSMFSIIVLSENYASSRWCLEEMVKILECNRSKEERVLPIFYNVDPSDVRNHMGKFGEALAKHEENLEENGERVKIWRDALTEVANLSGWDSRNKNEPLLIKEIVIKLLKKLLNTWTSDTEENLVGIQSRIQKLRMLLCLQSDDVRMVGICGMGGIGKTTLARAIYSQVSNQFEACSFLEIANDFKEQDLTSLAEKLLSQLLQEENLKIKGSTSIKARLHSRKVLVVLDNVNNLTILEHLAGNQDWFGQGSRIIVTTRDQRLLIQHKVDYYEVAEFNGDEAFEFLKHHSLKYELLENDLQELSREIIFYAKGLPLALRVLGSLLFGMNKDEWRDYLVKLKSTPNIEIQEVLRLSYDRLDDEEKNIFLDIACFFKGEDKDHVVEILKGCGFSAKCGIKTLINKSLITINFANKLEMHDLIQEMGKGIVRQECPKEPERRSRLWEHEDIFDVLKRNMGSEKIEGIFLNLSHLEDTLDFTIEAFAGMKKLRLLKVYNSKSISRDFRDTFNNKVNCRVRFAHEFKFCSNDLRYLYWHGYSLKSLPKDFSPKHLVELSMPYSHIKKLWKGIKVLERLKSIDLSHSKYLIQTPDFSGITNLERLVLEGCINLPKVHPSLGVLKKLNFLSLKNCTMLRRLPSSTCSLKSLETFILSGCSKFEEFPENFGNLEMLKELHADGIVVRVLPPSFFSMRNLEKLSFGGCKGPASASWLWPKRSSNSICFTVPSSSNLCSLKNLDLSYCNISDGANVSGLGFLVSLEWLNLSGNNFVTLPNMSGLSHLETLRLGNCKRLEALSQLPSSIRSLNAKNCTSLGTTELLNLLLTTKVHTLLRPRELESLDSTFGVVIPGSRIPDWIRYQSSRNVIEADLPLNWSTNCLGFALALVFGGRFPVAYDDWFWARVFLDFGTCRRSFETGISFPMENSVFAEGDHVVLTFAPVQPSLSPHQVIHIKATFAIMSVPNYYEIKRCGLGLMYVNEEVQK

Target ORF information:

RefSeq Version	XM_019217900.1
Organism	Vitis vinifera(wine grape)
Definition	Vitis vinifera TMV resistance protein N-like (LOC104877973), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_019217900.1

ORF Insert Sequence:

ATGGCTGCTG CTTTTTCTTC TTCTCAGAAG AGCTATGATG TCTTCCTCAG TTTCAGAGGA 
GACGACACCC GCAACAATTT CACTGCCCAT CTCCTTCAGG AGTTGCGCAC CAAAGGAATC 
AACACTTTCT TTGATGAGGA TAAGCTCGAG AAAGGTCGAG TCATATCCCC TGCACTCATT 
ACAGCTATTG AAAACTCCAT GTTTTCTATC ATTGTTTTGT CGGAAAACTA TGCATCTTCT 
AGGTGGTGCT TGGAGGAGAT GGTCAAGATA CTAGAATGCA ATAGATCCAA GGAGGAGAGG 
GTTCTACCAA TTTTCTACAA CGTGGATCCA TCAGATGTGC GGAATCACAT GGGAAAATTT 
GGAGAAGCCT TGGCTAAACA TGAAGAGAAT TTGGAGGAGA ACGGGGAGAG GGTGAAAATT 
TGGAGGGACG CTCTGACTGA AGTTGCAAAT TTATCTGGCT GGGATTCAAG GAACAAGAAT 
GAGCCTCTGC TAATAAAAGA AATCGTTATA AAACTTTTGA AGAAGTTGTT AAATACATGG 
ACAAGTGATA CTGAAGAGAA CCTGGTTGGA ATACAGTCCC GCATTCAAAA ACTGAGAATG 
CTATTGTGTT TACAATCAGA TGATGTTCGG ATGGTAGGAA TTTGTGGTAT GGGTGGAATA 
GGGAAAACAA CCCTTGCTAG AGCTATTTAT AGCCAAGTTT CTAACCAATT TGAAGCTTGC 
TCCTTTCTTG AAATTGCAAA TGATTTCAAA GAGCAGGATT TAACTAGTTT AGCAGAGAAA 
CTTCTTTCTC AATTATTGCA GGAAGAAAAT CTCAAAATAA AAGGATCCAC TTCTATAAAA 
GCAAGGCTCC ACTCAAGAAA GGTCCTTGTT GTTCTTGATA ATGTGAATAA TCTAACAATA 
TTGGAACATT TAGCTGGAAA TCAAGATTGG TTTGGTCAAG GGAGTAGAAT TATCGTAACT 
ACTCGGGATC AACGTTTGTT AATTCAACAT AAAGTTGATT ATTATGAGGT TGCAGAATTT 
AATGGTGATG AAGCATTTGA GTTCCTCAAA CATCATTCGT TAAAATATGA ACTTCTTGAA 
AATGATTTAC AGGAGCTTTC AAGAGAAATA ATATTTTATG CTAAAGGCCT TCCATTGGCT 
CTTAGAGTTT TAGGTTCTCT TTTATTTGGC ATGAACAAAG ACGAGTGGAG AGATTACTTG 
GTCAAACTAA AAAGTACTCC TAATATAGAA ATTCAGGAAG TACTTAGATT AAGTTACGAT 
AGGCTAGATG ATGAAGAGAA GAATATATTT TTGGATATTG CATGTTTCTT TAAAGGAGAG 
GACAAAGATC ATGTGGTGGA AATACTGAAG GGTTGTGGCT TCTCTGCAAA ATGTGGAATA 
AAAACTCTCA TTAACAAGTC TCTCATAACT ATTAATTTTG CTAACAAGTT GGAGATGCAT 
GACTTAATAC AAGAAATGGG TAAGGGTATT GTTCGCCAAG AATGTCCAAA AGAGCCTGAA 
AGACGAAGTA GATTGTGGGA ACATGAAGAT ATCTTTGATG TGTTGAAAAG AAATATGGGA 
AGTGAAAAAA TTGAAGGCAT ATTCCTCAAC TTGTCTCATT TAGAAGATAC ACTAGACTTC 
ACCATTGAAG CCTTTGCTGG GATGAAAAAA CTTAGATTGC TCAAAGTCTA TAATTCTAAG 
AGTATTTCAA GAGACTTCAG AGATACCTTT AACAACAAGG TGAATTGTAG AGTGCGGTTT 
GCCCATGAAT TTAAGTTTTG TTCCAATGAT TTGAGGTACT TATATTGGCA TGGATACTCT 
TTGAAATCAT TACCAAAGGA TTTCAGCCCA AAGCATCTTG TTGAGCTCAG TATGCCTTAT 
AGTCACATTA AAAAACTTTG GAAAGGAATC AAGGTTCTTG AAAGGTTAAA ATCCATAGAT 
CTCAGCCACT CCAAGTACTT AATACAAACT CCAGATTTCT CAGGGATCAC CAACCTTGAA 
CGTCTAGTTT TAGAAGGTTG TATAAATTTG CCTAAGGTTC ACCCATCACT TGGAGTTTTG 
AAGAAGCTAA ATTTCTTGAG TTTAAAAAAT TGCACAATGC TTAGAAGGCT TCCAAGCAGC 
ACTTGTAGTT TGAAATCTCT TGAAACCTTT ATTCTTTCTG GTTGCTCCAA ATTTGAAGAA 
TTTCCAGAGA ATTTTGGGAA CTTAGAAATG TTGAAGGAGC TTCACGCAGA TGGAATTGTG 
GTAAGAGTAC TACCCCCCTC CTTTTTTTCC ATGAGAAACC TTGAAAAGTT ATCTTTCGGT 
GGATGTAAAG GACCAGCATC TGCCTCATGG TTGTGGCCAA AAAGAAGTTC AAATTCTATT 
TGTTTCACGG TACCTTCTTC CTCAAACTTA TGCTCTTTAA AGAACCTAGA TTTAAGTTAC 
TGCAATATAT CAGATGGAGC AAATGTTAGT GGCCTTGGCT TCTTAGTTTC ACTTGAATGG 
TTAAATTTAA GTGGAAACAA CTTTGTCACT TTGCCTAACA TGAGTGGACT CTCTCACCTG 
GAAACGCTTC GGTTGGGAAA TTGTAAAAGA CTAGAGGCAC TTTCACAGCT TCCATCAAGC 
ATACGTTCTT TAAATGCAAA AAATTGCACA TCGTTGGGAA CAACTGAATT ACTCAACTTG 
CTACTTACAA CGAAGGTGCA TACTCTGCTG AGACCGCGGG AACTCGAATC CCTAGATTCT 
ACATTCGGTG TTGTGATTCC TGGGAGTAGA ATACCAGATT GGATCAGGTA TCAGAGCTCG 
AGGAATGTAA TTGAAGCAGA CCTACCCCTA AATTGGTCTA CCAACTGCCT GGGTTTTGCA 
TTGGCTCTTG TCTTCGGTGG CCGGTTTCCT GTCGCTTATG ATGATTGGTT TTGGGCACGT 
GTTTTCTTGG ACTTTGGCAC ATGTCGCCGT TCTTTCGAAA CTGGTATTTC TTTCCCCATG 
GAAAATAGTG TTTTCGCTGA AGGGGATCAT GTGGTTCTGA CTTTTGCACC AGTTCAACCC 
TCACTCAGTC CGCATCAAGT CATTCACATC AAGGCTACAT TTGCAATAAT GTCCGTGCCG 
AACTATTATG AAATTAAGAG ATGTGGGTTA GGTTTAATGT ATGTTAATGA AGAGGTCCAA 
AAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

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LOC104877973 cDNA ORF clone, Vitis vinifera(wine grape)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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