LOC100248326 cDNA ORF clone, Vitis vinifera(wine grape)

Gene
Clones
gRNAs

Summary

Gene Symbol	LOC100248326
Entrez Gene ID	100248326
Full Name	calcium-transporting ATPase 4, endoplasmic reticulum-type
Gene Type	protein-coding
Organism	Vitis vinifera(wine grape)

mRNA and Protein(s)

mRNA	Protein	Name
XM_019221769.1	XP_019077314.1	calcium-transporting ATPase 4, endoplasmic reticulum-type
XM_002277270.3	XP_002277306.1	calcium-transporting ATPase 4, endoplasmic reticulum-type

Pathways from BioSystems
Gene Ontology

The following LOC100248326 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC100248326 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OVa08784	XM_002277270.3 Latest version!	Vitis vinifera calcium-transporting ATPase 4, endoplasmic reticulum-type (LOC100248326), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OVa08784	XM_019221769.1 Latest version!	Vitis vinifera calcium-transporting ATPase 4, endoplasmic reticulum-type (LOC100248326), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LOC100248326 ( XM_019221769.1 ) cDNA ORF clone, Vitis vinifera(wine grape) -> XP_019077314.1 Vitis vinifera calcium-transporting ATPase 4, endoplasmic reticulum-type (LOC100248326), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OVa08784
Clone ID Related Accession (Same CDS sequence)	XM_019221769.1 , XM_002277270.3
Accession Version	XM_019221769.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3192bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1479916800000
Organism	Vitis vinifera(wine grape)
Product	calcium-transporting ATPase 4, endoplasmic reticulum-type
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_012014.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Vitis vinifera Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGAAAGG GTGGGGAGGA CTATGGGAAG CGGGAGGTTT CGAGCTCTAA GGTGAGTGGC 
CCGGAAGTGT TTCCGGCGTG GGGGAGGGAG GTTCAGGAAT GTGAGAAGCA TTATGGGGTG 
AGTAGGAGAT CGGGGTTGAG TTCCAGCGAT GTGGAGAAGC GGCGCAAGAT CTACGGGTTG 
AATGAATTGG AGAAGCACGA GGGGCCGTCG ATTTGGAGTT TAATTCTGGA GCAGTTTCAG 
GATACTCTGG TTCGGATTCT ACTCGTGGCC GCGGTGATTT CGTTCGTGCT CGCTTGGTAC 
GATGGCGAGG AGGGGGGTGA GACGGAGATT ACGGCGTTTG TCGAGCCTCT GGTGATTTTC 
TTGATATTGA TTGCGAATGC TATTGTTGGG GTTTGGCAAG AGAACAATGC GGAGAAGGCG 
TTGGAGGCCC TTAAGGAGAT TCAATCTGAG CAGGCTGCCG TGATCCGCAA TAATCAACGC 
ATTCCCAATT TGCCGGCGAA GGAGCTTGTG CCCGGTGACA TCGTGGAACT TAAGGTCGGG 
GATAAGGTGC CTGCAGATAT GCGGGTTGTG GAATTGATTA GCTCAACTCT GCGTCTGGAA 
CAGGGGTCGT TGACCGGAGA GAGTGAAGCA GTGAATAAGA CAAATAAGCC TGTCCCCGAG 
GATGCCGACA TTCAAGGGAA AAGGTGTATG GTTTTTGCAG GAACGACTGT TGTGAATGGG 
AACTGCATTT GCTTGGTGAC CCAGACGGGT ATGGAGACAG AGATTGGAAA GGTGCATACT 
CAAATTCATG TGGCTTCCCA GAGTGAGGAA GACACGCCTT TGAAGAAAAA GTTGAATGAG 
TTTGGAGAGT CGCTGACAGT GATAATTGGA GTGATATGTG CGTTGGTATG GCTTATCAAT 
GTGAAGTACT TCCTTAATTG GGAGTATGTT GATGGGTGGC CGAGTAATTT CAAGTTCTCA 
TTTGAGAAGT GCACCTATTA CTTCAAGATT GCAGTGGCAT TGGCTGTTGC TGCCATTCCG 
GAAGGTTTAC CGGCAGTTAT CACCACTTGC TTGGCTCTAG GCACACGCAA GATGGCTCAG 
AAGAATGCTC TTGTGCGAAA GCTGCCAAGT GTTGAAACTC TGGGCTGCAC AACTGTGATT 
TGTTCAGATA AAACGGGTAC CTTAACTACT AACCAGATGG CAGTGGCAAA GCTGGTGGCC 
ATGGGTTCTA GAGCAGGAGC ACTTCGAAAA TTCAGAGTGG ATGGGACCAC ATACAGTCCC 
TTTGATGGAA AAATACACGA CTGGCCCTGT GGTCGCATGG ATGCTAATCT TCAAATGATT 
GCGAAGATTT CTGCTGTATG CAATGACGCT GGTGTGGCAC AGTCAGAGCA TAAGTATGTT 
GCCAATGGAA TGCCAACAGA GGCAGCTCTG AAGGTTCTGG TCGAGAAAAT GGGTCCTCCT 
GCAGTCGATG ATGACAAATC ATTTTCAAGT TCTGGTGATC TGCTACGTTG CTGCCAACGC 
TGGAATGAAA ATGAACGTCG AATTGCTACT CTTGAGTTTG ATCGTGATCG CAAGTCTATG 
GGGGTTATTG TCAATTCTCA CTCAGGAAAA AAGTCATTGC TAGTGAAGGG TGCAGTAGAA 
AATCTATTGG AGAGAAGCAA CTCTGTTCAG TTGCTTGATG GTTCTGTTGT AGAACTGGGT 
GATAATTCTA GGAGCCTTAT TTTAGAAGCA CTTCACGAAA TGTCAAGTGG TGCATTACGT 
TGTTTGGGTT TTGCATACAA AGATGAGCTC CCTGACTTTG CAACATATGA TGGTGATGAA 
AATCATCCAG CTCATGGTCT TCTACTCAAT CCTGCCAACT ATTCATCAAT TGAGAGGAAC 
CTTACTTTTG TAGGCTTGGT TGGGCTGAGG GATCCTCCTC GTGCAGAGGT TCACCAAGCA 
ATTGAAGACT GCAGAGCAGC CGGTATTCGT GTTATGGTCA TCACAGGCGA TAACAAGAAT 
ACAGCAGAAG CTATTTGTCA TGAAATAGGT GTATTTGGAC CTAATGAGGA CATTAGGTCG 
AAAAGCTTAA CTGGGAAAGA GTTCATGGAA CTTCGGGATC AGAAAGCACA TCTGAGACAA 
AATGGTGGGC TTCTGTTCTC TAGGGCTGAA CCTAGGCATA AACAAGAGAT CGTGAGGCTG 
CTTAAAGAAG ATGGGGAAGT GGTCGCCATG ACTGGTGATG GAGTGAATGA TGCGCCTGCC 
TTGAAACTGG CTGATATTGG CATTGCTATG GGTATTGCTG GGACAGAGGT TGCTAAAGAA 
GCCTCTGATA TGGTGTTGGC AGACGATAAT TTTAGTACAA TAGTTGCTGC TGTTGGGGAA 
GGGCGATCTA TCTACAACAA TATGAAGGCT TTTATCAGGT ATATGATTTC CTCAAATATT 
GGTGAGGTTG CATCCATATT TTTAACAGCA GCTTTAGGGA TCCCTGAAGG GTTGATACCT 
GTTCAGCTTT TATGGGTCAA CCTTGTTACT GATGGCCCCC CTGCAACTGC TTTGGGATTC 
AATCCACCAG ATAGGGACAT AATGAAGAAA CCACCTCGTA GAAGTGATGA CTCACTCATC 
AGTGCTTGGA TCTTGTTCCG CTATCTGGTA ATTGGATTGT ATGTTGGAAT AGCAACCGTT 
GGTGTATTTG TCATTTGGTA CACGCATAGT TCCTTCTTGG GCATTGACCT CAGTGGGGAT 
GGCCACACCC TTGTCACCTA CACCCAGCTT GCTGACTGGG GCCAATGCTC ATCCTGGGAG 
AATTTCACTA TTTCACCCTT CACAGCTGGT GCTCAGGTGT TCACTTTCAA CGACAATCCC 
TGTGATTACT TTCAGGGTGG CAAAGTGAAG GCCACTACAC TGTCTCTTTC AGTCCTGGTT 
GCCATTGAAA TGTTCAATTC CCTCAATGCC CTTTCAGAGG ATGGGAGTCT TCTAGTGATG 
CCTCCATGGG TCAATCCGTG GCTCCTGGTA GCCATGTCTG TCTCATTTGG CTTGCACTTT 
CTGATTCTGT ATGTGCCGGT TCTTGCTCAA GTATTCGGGA TTGTGCCCCT CAGTTTGAAC 
GAATGGCTAT TGGTTTTGGC GGTTGCATTC CCAGTGATTT TAATTGATGA GATTCTAAAG 
TTGGTGGGGC GATGTACAAG CGGTTTTCAA ACATCCAGCA CAAGGAAATC CTTGAAGCCG 
AAGTCAGAAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_019077314.1
CDS	302..3493
Translation	MGKGGEDYGKREVSSSKVSGPEVFPAWGREVQECEKHYGVSRRSGLSSSDVEKRRKIYGLNELEKHEGPSIWSLILEQFQDTLVRILLVAAVISFVLAWYDGEEGGETEITAFVEPLVIFLILIANAIVGVWQENNAEKALEALKEIQSEQAAVIRNNQRIPNLPAKELVPGDIVELKVGDKVPADMRVVELISSTLRLEQGSLTGESEAVNKTNKPVPEDADIQGKRCMVFAGTTVVNGNCICLVTQTGMETEIGKVHTQIHVASQSEEDTPLKKKLNEFGESLTVIIGVICALVWLINVKYFLNWEYVDGWPSNFKFSFEKCTYYFKIAVALAVAAIPEGLPAVITTCLALGTRKMAQKNALVRKLPSVETLGCTTVICSDKTGTLTTNQMAVAKLVAMGSRAGALRKFRVDGTTYSPFDGKIHDWPCGRMDANLQMIAKISAVCNDAGVAQSEHKYVANGMPTEAALKVLVEKMGPPAVDDDKSFSSSGDLLRCCQRWNENERRIATLEFDRDRKSMGVIVNSHSGKKSLLVKGAVENLLERSNSVQLLDGSVVELGDNSRSLILEALHEMSSGALRCLGFAYKDELPDFATYDGDENHPAHGLLLNPANYSSIERNLTFVGLVGLRDPPRAEVHQAIEDCRAAGIRVMVITGDNKNTAEAICHEIGVFGPNEDIRSKSLTGKEFMELRDQKAHLRQNGGLLFSRAEPRHKQEIVRLLKEDGEVVAMTGDGVNDAPALKLADIGIAMGIAGTEVAKEASDMVLADDNFSTIVAAVGEGRSIYNNMKAFIRYMISSNIGEVASIFLTAALGIPEGLIPVQLLWVNLVTDGPPATALGFNPPDRDIMKKPPRRSDDSLISAWILFRYLVIGLYVGIATVGVFVIWYTHSSFLGIDLSGDGHTLVTYTQLADWGQCSSWENFTISPFTAGAQVFTFNDNPCDYFQGGKVKATTLSLSVLVAIEMFNSLNALSEDGSLLVMPPWVNPWLLVAMSVSFGLHFLILYVPVLAQVFGIVPLSLNEWLLVLAVAFPVILIDEILKLVGRCTSGFQTSSTRKSLKPKSE

Target ORF information:

RefSeq Version	XM_019221769.1
Organism	Vitis vinifera(wine grape)
Definition	Vitis vinifera calcium-transporting ATPase 4, endoplasmic reticulum-type (LOC100248326), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_019221769.1

ORF Insert Sequence:

ATGGGAAAGG GTGGGGAGGA CTATGGGAAG CGGGAGGTTT CGAGCTCTAA GGTGAGTGGC 
CCGGAAGTGT TTCCGGCGTG GGGGAGGGAG GTTCAGGAAT GTGAGAAGCA TTATGGGGTG 
AGTAGGAGAT CGGGGTTGAG TTCCAGCGAT GTGGAGAAGC GGCGCAAGAT CTACGGGTTG 
AATGAATTGG AGAAGCACGA GGGGCCGTCG ATTTGGAGTT TAATTCTGGA GCAGTTTCAG 
GATACTCTGG TTCGGATTCT ACTCGTGGCC GCGGTGATTT CGTTCGTGCT CGCTTGGTAC 
GATGGCGAGG AGGGGGGTGA GACGGAGATT ACGGCGTTTG TCGAGCCTCT GGTGATTTTC 
TTGATATTGA TTGCGAATGC TATTGTTGGG GTTTGGCAAG AGAACAATGC GGAGAAGGCG 
TTGGAGGCCC TTAAGGAGAT TCAATCTGAG CAGGCTGCCG TGATCCGCAA TAATCAACGC 
ATTCCCAATT TGCCGGCGAA GGAGCTTGTG CCCGGTGACA TCGTGGAACT TAAGGTCGGG 
GATAAGGTGC CTGCAGATAT GCGGGTTGTG GAATTGATTA GCTCAACTCT GCGTCTGGAA 
CAGGGGTCGT TGACCGGAGA GAGTGAAGCA GTGAATAAGA CAAATAAGCC TGTCCCCGAG 
GATGCCGACA TTCAAGGGAA AAGGTGTATG GTTTTTGCAG GAACGACTGT TGTGAATGGG 
AACTGCATTT GCTTGGTGAC CCAGACGGGT ATGGAGACAG AGATTGGAAA GGTGCATACT 
CAAATTCATG TGGCTTCCCA GAGTGAGGAA GACACGCCTT TGAAGAAAAA GTTGAATGAG 
TTTGGAGAGT CGCTGACAGT GATAATTGGA GTGATATGTG CGTTGGTATG GCTTATCAAT 
GTGAAGTACT TCCTTAATTG GGAGTATGTT GATGGGTGGC CGAGTAATTT CAAGTTCTCA 
TTTGAGAAGT GCACCTATTA CTTCAAGATT GCAGTGGCAT TGGCTGTTGC TGCCATTCCG 
GAAGGTTTAC CGGCAGTTAT CACCACTTGC TTGGCTCTAG GCACACGCAA GATGGCTCAG 
AAGAATGCTC TTGTGCGAAA GCTGCCAAGT GTTGAAACTC TGGGCTGCAC AACTGTGATT 
TGTTCAGATA AAACGGGTAC CTTAACTACT AACCAGATGG CAGTGGCAAA GCTGGTGGCC 
ATGGGTTCTA GAGCAGGAGC ACTTCGAAAA TTCAGAGTGG ATGGGACCAC ATACAGTCCC 
TTTGATGGAA AAATACACGA CTGGCCCTGT GGTCGCATGG ATGCTAATCT TCAAATGATT 
GCGAAGATTT CTGCTGTATG CAATGACGCT GGTGTGGCAC AGTCAGAGCA TAAGTATGTT 
GCCAATGGAA TGCCAACAGA GGCAGCTCTG AAGGTTCTGG TCGAGAAAAT GGGTCCTCCT 
GCAGTCGATG ATGACAAATC ATTTTCAAGT TCTGGTGATC TGCTACGTTG CTGCCAACGC 
TGGAATGAAA ATGAACGTCG AATTGCTACT CTTGAGTTTG ATCGTGATCG CAAGTCTATG 
GGGGTTATTG TCAATTCTCA CTCAGGAAAA AAGTCATTGC TAGTGAAGGG TGCAGTAGAA 
AATCTATTGG AGAGAAGCAA CTCTGTTCAG TTGCTTGATG GTTCTGTTGT AGAACTGGGT 
GATAATTCTA GGAGCCTTAT TTTAGAAGCA CTTCACGAAA TGTCAAGTGG TGCATTACGT 
TGTTTGGGTT TTGCATACAA AGATGAGCTC CCTGACTTTG CAACATATGA TGGTGATGAA 
AATCATCCAG CTCATGGTCT TCTACTCAAT CCTGCCAACT ATTCATCAAT TGAGAGGAAC 
CTTACTTTTG TAGGCTTGGT TGGGCTGAGG GATCCTCCTC GTGCAGAGGT TCACCAAGCA 
ATTGAAGACT GCAGAGCAGC CGGTATTCGT GTTATGGTCA TCACAGGCGA TAACAAGAAT 
ACAGCAGAAG CTATTTGTCA TGAAATAGGT GTATTTGGAC CTAATGAGGA CATTAGGTCG 
AAAAGCTTAA CTGGGAAAGA GTTCATGGAA CTTCGGGATC AGAAAGCACA TCTGAGACAA 
AATGGTGGGC TTCTGTTCTC TAGGGCTGAA CCTAGGCATA AACAAGAGAT CGTGAGGCTG 
CTTAAAGAAG ATGGGGAAGT GGTCGCCATG ACTGGTGATG GAGTGAATGA TGCGCCTGCC 
TTGAAACTGG CTGATATTGG CATTGCTATG GGTATTGCTG GGACAGAGGT TGCTAAAGAA 
GCCTCTGATA TGGTGTTGGC AGACGATAAT TTTAGTACAA TAGTTGCTGC TGTTGGGGAA 
GGGCGATCTA TCTACAACAA TATGAAGGCT TTTATCAGGT ATATGATTTC CTCAAATATT 
GGTGAGGTTG CATCCATATT TTTAACAGCA GCTTTAGGGA TCCCTGAAGG GTTGATACCT 
GTTCAGCTTT TATGGGTCAA CCTTGTTACT GATGGCCCCC CTGCAACTGC TTTGGGATTC 
AATCCACCAG ATAGGGACAT AATGAAGAAA CCACCTCGTA GAAGTGATGA CTCACTCATC 
AGTGCTTGGA TCTTGTTCCG CTATCTGGTA ATTGGATTGT ATGTTGGAAT AGCAACCGTT 
GGTGTATTTG TCATTTGGTA CACGCATAGT TCCTTCTTGG GCATTGACCT CAGTGGGGAT 
GGCCACACCC TTGTCACCTA CACCCAGCTT GCTGACTGGG GCCAATGCTC ATCCTGGGAG 
AATTTCACTA TTTCACCCTT CACAGCTGGT GCTCAGGTGT TCACTTTCAA CGACAATCCC 
TGTGATTACT TTCAGGGTGG CAAAGTGAAG GCCACTACAC TGTCTCTTTC AGTCCTGGTT 
GCCATTGAAA TGTTCAATTC CCTCAATGCC CTTTCAGAGG ATGGGAGTCT TCTAGTGATG 
CCTCCATGGG TCAATCCGTG GCTCCTGGTA GCCATGTCTG TCTCATTTGG CTTGCACTTT 
CTGATTCTGT ATGTGCCGGT TCTTGCTCAA GTATTCGGGA TTGTGCCCCT CAGTTTGAAC 
GAATGGCTAT TGGTTTTGGC GGTTGCATTC CCAGTGATTT TAATTGATGA GATTCTAAAG 
TTGGTGGGGC GATGTACAAG CGGTTTTCAA ACATCCAGCA CAAGGAAATC CTTGAAGCCG 
AAGTCAGAAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC100248326 ( XM_002277270.3 ) cDNA ORF clone, Vitis vinifera(wine grape) -> XP_002277306.1 Vitis vinifera calcium-transporting ATPase 4, endoplasmic reticulum-type (LOC100248326), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OVa08784
Clone ID Related Accession (Same CDS sequence)	XM_019221769.1 , XM_002277270.3
Accession Version	XM_002277270.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3192bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1479916800000
Organism	Vitis vinifera(wine grape)
Product	calcium-transporting ATPase 4, endoplasmic reticulum-type
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_012014.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Nov 25, 2016 this sequence version replaced XM_002277270.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Vitis vinifera Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGAAAGG GTGGGGAGGA CTATGGGAAG CGGGAGGTTT CGAGCTCTAA GGTGAGTGGC 
CCGGAAGTGT TTCCGGCGTG GGGGAGGGAG GTTCAGGAAT GTGAGAAGCA TTATGGGGTG 
AGTAGGAGAT CGGGGTTGAG TTCCAGCGAT GTGGAGAAGC GGCGCAAGAT CTACGGGTTG 
AATGAATTGG AGAAGCACGA GGGGCCGTCG ATTTGGAGTT TAATTCTGGA GCAGTTTCAG 
GATACTCTGG TTCGGATTCT ACTCGTGGCC GCGGTGATTT CGTTCGTGCT CGCTTGGTAC 
GATGGCGAGG AGGGGGGTGA GACGGAGATT ACGGCGTTTG TCGAGCCTCT GGTGATTTTC 
TTGATATTGA TTGCGAATGC TATTGTTGGG GTTTGGCAAG AGAACAATGC GGAGAAGGCG 
TTGGAGGCCC TTAAGGAGAT TCAATCTGAG CAGGCTGCCG TGATCCGCAA TAATCAACGC 
ATTCCCAATT TGCCGGCGAA GGAGCTTGTG CCCGGTGACA TCGTGGAACT TAAGGTCGGG 
GATAAGGTGC CTGCAGATAT GCGGGTTGTG GAATTGATTA GCTCAACTCT GCGTCTGGAA 
CAGGGGTCGT TGACCGGAGA GAGTGAAGCA GTGAATAAGA CAAATAAGCC TGTCCCCGAG 
GATGCCGACA TTCAAGGGAA AAGGTGTATG GTTTTTGCAG GAACGACTGT TGTGAATGGG 
AACTGCATTT GCTTGGTGAC CCAGACGGGT ATGGAGACAG AGATTGGAAA GGTGCATACT 
CAAATTCATG TGGCTTCCCA GAGTGAGGAA GACACGCCTT TGAAGAAAAA GTTGAATGAG 
TTTGGAGAGT CGCTGACAGT GATAATTGGA GTGATATGTG CGTTGGTATG GCTTATCAAT 
GTGAAGTACT TCCTTAATTG GGAGTATGTT GATGGGTGGC CGAGTAATTT CAAGTTCTCA 
TTTGAGAAGT GCACCTATTA CTTCAAGATT GCAGTGGCAT TGGCTGTTGC TGCCATTCCG 
GAAGGTTTAC CGGCAGTTAT CACCACTTGC TTGGCTCTAG GCACACGCAA GATGGCTCAG 
AAGAATGCTC TTGTGCGAAA GCTGCCAAGT GTTGAAACTC TGGGCTGCAC AACTGTGATT 
TGTTCAGATA AAACGGGTAC CTTAACTACT AACCAGATGG CAGTGGCAAA GCTGGTGGCC 
ATGGGTTCTA GAGCAGGAGC ACTTCGAAAA TTCAGAGTGG ATGGGACCAC ATACAGTCCC 
TTTGATGGAA AAATACACGA CTGGCCCTGT GGTCGCATGG ATGCTAATCT TCAAATGATT 
GCGAAGATTT CTGCTGTATG CAATGACGCT GGTGTGGCAC AGTCAGAGCA TAAGTATGTT 
GCCAATGGAA TGCCAACAGA GGCAGCTCTG AAGGTTCTGG TCGAGAAAAT GGGTCCTCCT 
GCAGTCGATG ATGACAAATC ATTTTCAAGT TCTGGTGATC TGCTACGTTG CTGCCAACGC 
TGGAATGAAA ATGAACGTCG AATTGCTACT CTTGAGTTTG ATCGTGATCG CAAGTCTATG 
GGGGTTATTG TCAATTCTCA CTCAGGAAAA AAGTCATTGC TAGTGAAGGG TGCAGTAGAA 
AATCTATTGG AGAGAAGCAA CTCTGTTCAG TTGCTTGATG GTTCTGTTGT AGAACTGGGT 
GATAATTCTA GGAGCCTTAT TTTAGAAGCA CTTCACGAAA TGTCAAGTGG TGCATTACGT 
TGTTTGGGTT TTGCATACAA AGATGAGCTC CCTGACTTTG CAACATATGA TGGTGATGAA 
AATCATCCAG CTCATGGTCT TCTACTCAAT CCTGCCAACT ATTCATCAAT TGAGAGGAAC 
CTTACTTTTG TAGGCTTGGT TGGGCTGAGG GATCCTCCTC GTGCAGAGGT TCACCAAGCA 
ATTGAAGACT GCAGAGCAGC CGGTATTCGT GTTATGGTCA TCACAGGCGA TAACAAGAAT 
ACAGCAGAAG CTATTTGTCA TGAAATAGGT GTATTTGGAC CTAATGAGGA CATTAGGTCG 
AAAAGCTTAA CTGGGAAAGA GTTCATGGAA CTTCGGGATC AGAAAGCACA TCTGAGACAA 
AATGGTGGGC TTCTGTTCTC TAGGGCTGAA CCTAGGCATA AACAAGAGAT CGTGAGGCTG 
CTTAAAGAAG ATGGGGAAGT GGTCGCCATG ACTGGTGATG GAGTGAATGA TGCGCCTGCC 
TTGAAACTGG CTGATATTGG CATTGCTATG GGTATTGCTG GGACAGAGGT TGCTAAAGAA 
GCCTCTGATA TGGTGTTGGC AGACGATAAT TTTAGTACAA TAGTTGCTGC TGTTGGGGAA 
GGGCGATCTA TCTACAACAA TATGAAGGCT TTTATCAGGT ATATGATTTC CTCAAATATT 
GGTGAGGTTG CATCCATATT TTTAACAGCA GCTTTAGGGA TCCCTGAAGG GTTGATACCT 
GTTCAGCTTT TATGGGTCAA CCTTGTTACT GATGGCCCCC CTGCAACTGC TTTGGGATTC 
AATCCACCAG ATAGGGACAT AATGAAGAAA CCACCTCGTA GAAGTGATGA CTCACTCATC 
AGTGCTTGGA TCTTGTTCCG CTATCTGGTA ATTGGATTGT ATGTTGGAAT AGCAACCGTT 
GGTGTATTTG TCATTTGGTA CACGCATAGT TCCTTCTTGG GCATTGACCT CAGTGGGGAT 
GGCCACACCC TTGTCACCTA CACCCAGCTT GCTGACTGGG GCCAATGCTC ATCCTGGGAG 
AATTTCACTA TTTCACCCTT CACAGCTGGT GCTCAGGTGT TCACTTTCAA CGACAATCCC 
TGTGATTACT TTCAGGGTGG CAAAGTGAAG GCCACTACAC TGTCTCTTTC AGTCCTGGTT 
GCCATTGAAA TGTTCAATTC CCTCAATGCC CTTTCAGAGG ATGGGAGTCT TCTAGTGATG 
CCTCCATGGG TCAATCCGTG GCTCCTGGTA GCCATGTCTG TCTCATTTGG CTTGCACTTT 
CTGATTCTGT ATGTGCCGGT TCTTGCTCAA GTATTCGGGA TTGTGCCCCT CAGTTTGAAC 
GAATGGCTAT TGGTTTTGGC GGTTGCATTC CCAGTGATTT TAATTGATGA GATTCTAAAG 
TTGGTGGGGC GATGTACAAG CGGTTTTCAA ACATCCAGCA CAAGGAAATC CTTGAAGCCG 
AAGTCAGAAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_002277306.1
CDS	398..3589
Translation	MGKGGEDYGKREVSSSKVSGPEVFPAWGREVQECEKHYGVSRRSGLSSSDVEKRRKIYGLNELEKHEGPSIWSLILEQFQDTLVRILLVAAVISFVLAWYDGEEGGETEITAFVEPLVIFLILIANAIVGVWQENNAEKALEALKEIQSEQAAVIRNNQRIPNLPAKELVPGDIVELKVGDKVPADMRVVELISSTLRLEQGSLTGESEAVNKTNKPVPEDADIQGKRCMVFAGTTVVNGNCICLVTQTGMETEIGKVHTQIHVASQSEEDTPLKKKLNEFGESLTVIIGVICALVWLINVKYFLNWEYVDGWPSNFKFSFEKCTYYFKIAVALAVAAIPEGLPAVITTCLALGTRKMAQKNALVRKLPSVETLGCTTVICSDKTGTLTTNQMAVAKLVAMGSRAGALRKFRVDGTTYSPFDGKIHDWPCGRMDANLQMIAKISAVCNDAGVAQSEHKYVANGMPTEAALKVLVEKMGPPAVDDDKSFSSSGDLLRCCQRWNENERRIATLEFDRDRKSMGVIVNSHSGKKSLLVKGAVENLLERSNSVQLLDGSVVELGDNSRSLILEALHEMSSGALRCLGFAYKDELPDFATYDGDENHPAHGLLLNPANYSSIERNLTFVGLVGLRDPPRAEVHQAIEDCRAAGIRVMVITGDNKNTAEAICHEIGVFGPNEDIRSKSLTGKEFMELRDQKAHLRQNGGLLFSRAEPRHKQEIVRLLKEDGEVVAMTGDGVNDAPALKLADIGIAMGIAGTEVAKEASDMVLADDNFSTIVAAVGEGRSIYNNMKAFIRYMISSNIGEVASIFLTAALGIPEGLIPVQLLWVNLVTDGPPATALGFNPPDRDIMKKPPRRSDDSLISAWILFRYLVIGLYVGIATVGVFVIWYTHSSFLGIDLSGDGHTLVTYTQLADWGQCSSWENFTISPFTAGAQVFTFNDNPCDYFQGGKVKATTLSLSVLVAIEMFNSLNALSEDGSLLVMPPWVNPWLLVAMSVSFGLHFLILYVPVLAQVFGIVPLSLNEWLLVLAVAFPVILIDEILKLVGRCTSGFQTSSTRKSLKPKSE

Target ORF information:

RefSeq Version	XM_002277270.3
Organism	Vitis vinifera(wine grape)
Definition	Vitis vinifera calcium-transporting ATPase 4, endoplasmic reticulum-type (LOC100248326), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_002277270.3

ORF Insert Sequence:

ATGGGAAAGG GTGGGGAGGA CTATGGGAAG CGGGAGGTTT CGAGCTCTAA GGTGAGTGGC 
CCGGAAGTGT TTCCGGCGTG GGGGAGGGAG GTTCAGGAAT GTGAGAAGCA TTATGGGGTG 
AGTAGGAGAT CGGGGTTGAG TTCCAGCGAT GTGGAGAAGC GGCGCAAGAT CTACGGGTTG 
AATGAATTGG AGAAGCACGA GGGGCCGTCG ATTTGGAGTT TAATTCTGGA GCAGTTTCAG 
GATACTCTGG TTCGGATTCT ACTCGTGGCC GCGGTGATTT CGTTCGTGCT CGCTTGGTAC 
GATGGCGAGG AGGGGGGTGA GACGGAGATT ACGGCGTTTG TCGAGCCTCT GGTGATTTTC 
TTGATATTGA TTGCGAATGC TATTGTTGGG GTTTGGCAAG AGAACAATGC GGAGAAGGCG 
TTGGAGGCCC TTAAGGAGAT TCAATCTGAG CAGGCTGCCG TGATCCGCAA TAATCAACGC 
ATTCCCAATT TGCCGGCGAA GGAGCTTGTG CCCGGTGACA TCGTGGAACT TAAGGTCGGG 
GATAAGGTGC CTGCAGATAT GCGGGTTGTG GAATTGATTA GCTCAACTCT GCGTCTGGAA 
CAGGGGTCGT TGACCGGAGA GAGTGAAGCA GTGAATAAGA CAAATAAGCC TGTCCCCGAG 
GATGCCGACA TTCAAGGGAA AAGGTGTATG GTTTTTGCAG GAACGACTGT TGTGAATGGG 
AACTGCATTT GCTTGGTGAC CCAGACGGGT ATGGAGACAG AGATTGGAAA GGTGCATACT 
CAAATTCATG TGGCTTCCCA GAGTGAGGAA GACACGCCTT TGAAGAAAAA GTTGAATGAG 
TTTGGAGAGT CGCTGACAGT GATAATTGGA GTGATATGTG CGTTGGTATG GCTTATCAAT 
GTGAAGTACT TCCTTAATTG GGAGTATGTT GATGGGTGGC CGAGTAATTT CAAGTTCTCA 
TTTGAGAAGT GCACCTATTA CTTCAAGATT GCAGTGGCAT TGGCTGTTGC TGCCATTCCG 
GAAGGTTTAC CGGCAGTTAT CACCACTTGC TTGGCTCTAG GCACACGCAA GATGGCTCAG 
AAGAATGCTC TTGTGCGAAA GCTGCCAAGT GTTGAAACTC TGGGCTGCAC AACTGTGATT 
TGTTCAGATA AAACGGGTAC CTTAACTACT AACCAGATGG CAGTGGCAAA GCTGGTGGCC 
ATGGGTTCTA GAGCAGGAGC ACTTCGAAAA TTCAGAGTGG ATGGGACCAC ATACAGTCCC 
TTTGATGGAA AAATACACGA CTGGCCCTGT GGTCGCATGG ATGCTAATCT TCAAATGATT 
GCGAAGATTT CTGCTGTATG CAATGACGCT GGTGTGGCAC AGTCAGAGCA TAAGTATGTT 
GCCAATGGAA TGCCAACAGA GGCAGCTCTG AAGGTTCTGG TCGAGAAAAT GGGTCCTCCT 
GCAGTCGATG ATGACAAATC ATTTTCAAGT TCTGGTGATC TGCTACGTTG CTGCCAACGC 
TGGAATGAAA ATGAACGTCG AATTGCTACT CTTGAGTTTG ATCGTGATCG CAAGTCTATG 
GGGGTTATTG TCAATTCTCA CTCAGGAAAA AAGTCATTGC TAGTGAAGGG TGCAGTAGAA 
AATCTATTGG AGAGAAGCAA CTCTGTTCAG TTGCTTGATG GTTCTGTTGT AGAACTGGGT 
GATAATTCTA GGAGCCTTAT TTTAGAAGCA CTTCACGAAA TGTCAAGTGG TGCATTACGT 
TGTTTGGGTT TTGCATACAA AGATGAGCTC CCTGACTTTG CAACATATGA TGGTGATGAA 
AATCATCCAG CTCATGGTCT TCTACTCAAT CCTGCCAACT ATTCATCAAT TGAGAGGAAC 
CTTACTTTTG TAGGCTTGGT TGGGCTGAGG GATCCTCCTC GTGCAGAGGT TCACCAAGCA 
ATTGAAGACT GCAGAGCAGC CGGTATTCGT GTTATGGTCA TCACAGGCGA TAACAAGAAT 
ACAGCAGAAG CTATTTGTCA TGAAATAGGT GTATTTGGAC CTAATGAGGA CATTAGGTCG 
AAAAGCTTAA CTGGGAAAGA GTTCATGGAA CTTCGGGATC AGAAAGCACA TCTGAGACAA 
AATGGTGGGC TTCTGTTCTC TAGGGCTGAA CCTAGGCATA AACAAGAGAT CGTGAGGCTG 
CTTAAAGAAG ATGGGGAAGT GGTCGCCATG ACTGGTGATG GAGTGAATGA TGCGCCTGCC 
TTGAAACTGG CTGATATTGG CATTGCTATG GGTATTGCTG GGACAGAGGT TGCTAAAGAA 
GCCTCTGATA TGGTGTTGGC AGACGATAAT TTTAGTACAA TAGTTGCTGC TGTTGGGGAA 
GGGCGATCTA TCTACAACAA TATGAAGGCT TTTATCAGGT ATATGATTTC CTCAAATATT 
GGTGAGGTTG CATCCATATT TTTAACAGCA GCTTTAGGGA TCCCTGAAGG GTTGATACCT 
GTTCAGCTTT TATGGGTCAA CCTTGTTACT GATGGCCCCC CTGCAACTGC TTTGGGATTC 
AATCCACCAG ATAGGGACAT AATGAAGAAA CCACCTCGTA GAAGTGATGA CTCACTCATC 
AGTGCTTGGA TCTTGTTCCG CTATCTGGTA ATTGGATTGT ATGTTGGAAT AGCAACCGTT 
GGTGTATTTG TCATTTGGTA CACGCATAGT TCCTTCTTGG GCATTGACCT CAGTGGGGAT 
GGCCACACCC TTGTCACCTA CACCCAGCTT GCTGACTGGG GCCAATGCTC ATCCTGGGAG 
AATTTCACTA TTTCACCCTT CACAGCTGGT GCTCAGGTGT TCACTTTCAA CGACAATCCC 
TGTGATTACT TTCAGGGTGG CAAAGTGAAG GCCACTACAC TGTCTCTTTC AGTCCTGGTT 
GCCATTGAAA TGTTCAATTC CCTCAATGCC CTTTCAGAGG ATGGGAGTCT TCTAGTGATG 
CCTCCATGGG TCAATCCGTG GCTCCTGGTA GCCATGTCTG TCTCATTTGG CTTGCACTTT 
CTGATTCTGT ATGTGCCGGT TCTTGCTCAA GTATTCGGGA TTGTGCCCCT CAGTTTGAAC 
GAATGGCTAT TGGTTTTGGC GGTTGCATTC CCAGTGATTT TAATTGATGA GATTCTAAAG 
TTGGTGGGGC GATGTACAAG CGGTTTTCAA ACATCCAGCA CAAGGAAATC CTTGAAGCCG 
AAGTCAGAAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

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LOC100248326 cDNA ORF clone, Vitis vinifera(wine grape)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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