Tc00.1047053508577.40 cDNA ORF clone, Trypanosoma cruzi strain CL Brener

Gene
Clones
gRNAs

Summary

Gene Symbol	Tc00.1047053508577.40
Entrez Gene ID	3548059
Full Name	dispersed gene family protein 1 (DGF-1)
Gene Type	protein-coding
Organism	Trypanosoma cruzi strain CL Brener

mRNA and Protein(s)

mRNA	Protein	Name
XM_811127.1	XP_816220.1	dispersed gene family protein 1 (DGF-1)

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

The genome sequence of Trypanosoma cruzi, etiologic agent of Chagas disease.
El-Sayed NM, Myler PJ, Bartholomeu DC, Nilsson D, Aggarwal G, Tran AN, Ghedin E, Worthey EA, Delcher AL, Blandin G, Westenberger SJ, Caler E, Cerqueira GC, Branche C, Haas B, Anupama A, Arner E, Aslund L, Attipoe P, Bontempi E, Bringaud F, Burton P, Cadag E, Campbell DA, Carrington M, Crabtree J, Darban H, da Silveira JF, de Jong P, Edwards K, Englund PT, Fazelina G, Feldblyum T, Ferella M, Frasch AC, Gull K, Horn D, Hou L, Huang Y, Kindlund E, Klingbeil M, Kluge S, Koo H, Lacerda D, Levin MJ, Lorenzi H, Louie T, Machado CR, McCulloch R, McKenna A, Mizuno Y, Mottram JC, Nelson S, Ochaya S, Osoegawa K, Pai G, Parsons M, Pentony M, Pettersson U, Pop M, Ramirez JL, Rinta J, Robertson L, Salzberg SL, Sanchez DO, Seyler A, Sharma R, Shetty J, Simpson AJ, Sisk E, Tammi MT, Tarleton R, Teixeira S, Van Aken S, Vogt C, Ward PN, Wickstead B, Wortman J, White O, Fraser CM, Stuart KD, Andersson B
Science (New York, N.Y.)309(5733)409-15(2005 Jul)

Comparative genomics of trypanosomatid parasitic protozoa.
El-Sayed NM, Myler PJ, Blandin G, Berriman M, Crabtree J, Aggarwal G, Caler E, Renauld H, Worthey EA, Hertz-Fowler C, Ghedin E, Peacock C, Bartholomeu DC, Haas BJ, Tran AN, Wortman JR, Alsmark UC, Angiuoli S, Anupama A, Badger J, Bringaud F, Cadag E, Carlton JM, Cerqueira GC, Creasy T, Delcher AL, Djikeng A, Embley TM, Hauser C, Ivens AC, Kummerfeld SK, Pereira-Leal JB, Nilsson D, Peterson J, Salzberg SL, Shallom J, Silva JC, Sundaram J, Westenberger S, White O, Melville SE, Donelson JE, Andersson B, Stuart KD, Hall N
Science (New York, N.Y.)309(5733)404-9(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Tc00.1047053508577.40 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Tc00.1047053508577.40 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OTb10744	XM_811127.1 Latest version!	Trypanosoma cruzi strain CL Brener dispersed gene family protein 1 (DGF-1) partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Tc00.1047053508577.40 ( XM_811127.1 ) cDNA ORF clone, Trypanosoma cruzi strain CL Brener -> XP_816220.1 Trypanosoma cruzi strain CL Brener dispersed gene family protein 1 (DGF-1) partial mRNA.

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CloneID	OTb10744
Clone ID Related Accession (Same CDS sequence)	XM_811127.1
Accession Version	XM_811127.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 10380bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1225382400000
Organism	Trypanosoma cruzi strain CL Brener
Product	dispersed gene family protein 1 (DGF-1)
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NW_001849327). COMPLETENESS: incomplete on both ends.

ATGTGGTCTG CGGTGGGTGC GTGTCCGCGC AGCCGGCACC GTGTGCGGCG CAGGGCCATT 
GCGGCTGTGC GTGTGGCACT GCTTGTGGCG TTGGCGCTGG TTGCGGCGAC GGCGTTTATG 
CCCGCGGCGC ATGCGGTGGT GTTGCGACTG CGGGGCGGCA CGGTGGACAG AGCCATCACG 
GTTGGCCACG CCGTGGACAC GGTGCTGATG GACGGCGTGT ACGTCACGAA CGGCGTGGCC 
GTGGTGTTCG ACGTGCCGGC GATGCTTCCC GGCGCGCTGC GCATCGAACT GAGGAACTGC 
GTGTGCGACG GCGGTGCGCA GATCTACGTG CGGGGCTACA GCGGTGAGCC GGCGAGTGAC 
CGGAGCCTGG AGGTGAGCGT GAGCAGGCTG TCCGGCGGCT ACTGCTCGCT TGTGTTTGTG 
CACAGCCTGC CGGCACGCAC GAACGTGACG GTCCGTGACT CGACGATTGT GACGGCGGGG 
CCGATGCGCT ACTCCCAGCT GAGCGGGCTG ATGGACGCGG TGGCGTCGCC GCTTGTGCTG 
CACGCGACGT CGCTGTTGCA GACGCAGCTG CGTGTGAGCA ACACTGTGCT GAGGTATTTA 
CAGGCGGGCG GGTCGGCGGT GCACGTTGGC GGCGGTGTGG ACCTGCTGTC GAGCGCGGTG 
GTGCTTGACG GCGTGTTGCT GGAGGCGTCG GGCGATTCGA CCGCGTTCGC GTTGCATGTG 
GCGTCCTCGT CGCGTCTCAG TCTGCGGAGC CACTCGGTGT TCTCCGTGAC GAACATGTCG 
GTTGTGAGCA GTGGCGGCGG CTTTGTGCTT GGCGAGCGCC TCGCGGTGTC GGACTCGGTG 
CTGCGCTTCG TGGGCGTCGA GGGGTCTGTT GCGTCGTCGC TGGTGCGCTG CGACGGTGGG 
ACGGTGGGTG CTGGCGGGTG GCTGGAGCTG CACGACGTGT GGGCGGTGGG CGAGGCCTCG 
TCTGTGGCGT CGCTGTCGGG GGTGACGTTG AGTGGCGGCA CCGTGTCTAT TGCGCGCTGC 
GCTGCCACTG GCGCGACGCT TGTCTCCGGG CTGACGATCA CGAGCGGCGT CGTGTCGGTG 
CAATGCAACC GTGCCGGCGG CCGGGTGCTG CAGAGCAGCG GCGACTACCG CATGGCCGGC 
CTGCCCTCCG TGAGCGTGGT TCCGTGCGAC GGCTGTGCGG CTGCGCTGGC GTGCTTTGAT 
GCGCTGACGG CGTCGTTCTC CGACTGCGTG TGCGGCTGCC GTGCCGGCGG CGTGGGCGAG 
GCGTGCCTGC CGTTCGACGT GCCGCCTGCG AGGTCCGGCG GTGGTGGCGG TACTGCGCGG 
GACTGCGTGA GTGGCGTGAC GCTGATGGAG TCGGTGACGG TTGGCGGCGG GCGGGCGACG 
GCGTGCTTCG ACTCGGTGGT GTTCAGAGGG CCAATCACTG TGGCGGTGGA CCTGCGCTTG 
ATGGACGCGT TCGCTGACGC GCTGAACGTG ACGCTGCGCC ACTGCGTGCT TGCAGGTGGC 
GCGCAGCTGC GGATTGGTGG CCTCAGCGAG ATCACGGCGC ACCTGATGCC CCATGCCCTC 
GTCAACATGA CGAATGTGAC GTCGCTGGAG GGCACGATCG TGCTGCATGG CGCGATGCCG 
CCGAACTCGA GTGTGCTGCT GGCGAACTCG ACGCTGCGCG CGACGGTTGG CGGGTCGCGG 
TACGTGCCGA CGACCCCGGG CCACGAGAGA TTTCGGTACG GCCCCGCGCT TGTCCTGGAC 
GGTGTCCGGC TTCTGTCGAC GCGGTTTGTC ATGACGCGGT CGACGCTGGT GTGTGGCGGG 
AGCTCGTGCG CTGCTATCCT CGTGGAGCAC AGCCTTTGCG CGAAGCTGTC CAGCGTCTTC 
TACATGGACA ACTGCGCTGT CAAGTCGCGG AAGCATGTGA TTTACTCTCT TGCGTCGGAC 
CTGCGTGTTG GCGGCGACTC CGTGTTCTCC ATACAGAACA GCTCGTGGAG TGCGCCGAGC 
ACCGAGTACT ACAAAGGTGC GTGTGTGTTT AGGGGTGTTG TTGTGGCTGG CGGCAGCGTG 
CTGCAGATTG TGTCCAGCAC TTTCCGTCTC GGCTTTGCCA TGCTCATGGC AGCCACGCTG 
ACCGTGACTG GCGGCAGCTG GCTGGTGCAC CGCAACAACG AGTTCCACAG CGCCTACGTT 
GTGTACGTGG CCAACGAGAA CGGCGTGGCG TTTTGCGATC GGAGTGTGTG GTCGATACTT 
GACAACAACT TCACGTACGG CTCGTACTCG TCAACCATTG CGCATATGAC AACCAATTGG 
TCACCACCAT CCGACACGCA CCCGATCATC TACGGCATGT GCAACGAGGC AAGGGGCTCT 
CCTGTGACGG ATTACGGAGA AGAGCTCAAT ATTGTGGCGC CCGTGACGGT GCTGGACTGT 
GGTGCGTGCA CGATGAACGC CGTGTGCTTT GCTGCGAGGA CTAGTAGCAT CAGCGACTGT 
GAGTGTGTGT GTGCTGCTGG CGGCTACGGT GACACGTGCC TGCCGGCTGC GGTTCCGGAC 
GGCCTTGGGC CGCTCCCGCT CCCCGATGCG AAGGACACGG AGGTCCGCTG CGTGCACGGT 
GGCAGCATCA GCTCCGTGGA CTATCCTGAT CCCGGTGTGC GTGGTCTGTG CCTCGTCAAC 
GTGACCTTCA CCGCCCCGAT TGTGCTGGAC CTGTCGAGCT TCGACGTACC GCAGCAGACA 
CTCAACGTCA CGCTGCTGCA GTGCGTCCTC ATGGGCCTGT CGATCAAGGG GAGTGGTGCG 
CGTGTGCACG TGAGCGTGAC ATCCTCGTTG TTGGATTCTG GCGCATTGGA GTTTGAGGGT 
GATTTTGGCG CGAGCTCCCA GATACTGGTG GTGGGCAGTA CGATTGTGGC GACATCGGGC 
CACGCCATTT ACTTTGTGAA GTTTACTCTC AGTGCGAATA TGACGCTGCT GCTACTCGAC 
AACTACATTG AGGCGAATAG GCACGCAGTT TATTTTATTG ATGGTGTTGT TGTTGATGGT 
GGCAGGATCA TTGTGAAGGG AAATACTTTG AGGGCAACGG AAGTCGATGA CGGTGAGGAA 
TCATCTGTTT GCGTTACTTC TGTTGATGTG AGGAACGGCG GCTACTTTGA CGTGGAGAAC 
AACACGATGA GCTCGGTCAA TGGCGTTATT TTTTGGGGGG ATAATACCGT GAGGTCCGCG 
GGGCTGCTGC GAGTGGCGGA CTGCACCTTT GTTGGCGGGA CAGAAATTTT TGACCCCGCG 
CTGTTGCATT TGTCTGGCTC GGCGACACTT GAGGGAGGTG CGCAGTGGCG TTTGGAGGGC 
AACAGCGTGG TTGCCGCATC AGTATTAATT ATTTCGTATT CTTGGCACAA AATTCAGCTG 
TCGGGCAGCG GCACCACCGT GGTGCTTGCA CACAACCGTC AGGTGAACGA TAGTTATCCC 
TTTGCTGATC TGGCTCTGCC GAGCATGGTC AAGAAGCCAC CCGCACGGTT GGTCGTTGGG 
TGCAACCTGC AGGGCGATGA GGAGGCGTCG TACGACTATG TGTTTCCTGA GGGCGTGGTG 
GTGTTCAGGT GTGGCACATG CTACGACGAC GCGGCGTGCT ACATGCCCGG GACGGAGTTG 
GTGGACCGCG GCTCGTGCTC GTGCAGCTGC AAGGACGGAT GGCATGGCGC GTCGTGTCTT 
CCCTTCGAGG TGCCCGACAC GGCTGTGCCG CCCTTGCCGG AAAGAGCCGT GGACGGCGAC 
GCGAGCTGCG TGGTGAACCA GACGCTGACG GACCTCGTGC TGAACATGTG GAAGATACAC 
CACTGTTACG TGGATGTGAC ATTCAGCGGC GTGGGTGCAG TGCTGACGTT CTCCCTCAAC 
AGTATGCCGC TGCATCTCCC CATCAACATC ACGTTTACCG GGTGCACATT CCGCGACGGT 
GCCGTGCTGA AGTTTGTTGG AGGTGCTGAG GCTGTTGAGT CAACCGGCGT CCTGATCCGC 
GTGAGCCAGA CGGTGATGCG TTCCTCCGTT GTTGTGTTTG CACTTGCCTT CCCGCAGCAC 
TGTGACATCG CCGTTACGGA GGTTGATGCG GTGCAGTCCT CCGAGGTCCA TCTGCCGGAC 
GCCAGGAGGA ACAAGCTTAG CGTACTTTTA CTGATGAATG TTGTGCTGAG TGCGTCCTCG 
CTGCTGGTGA GCAACGTCAA GGCGCATGCA TTGAGGTATG ATGCGTTTGG TTTGTACTCG 
ACCGGGACGC TGACGCTGGT GGGTGGCAGC TCTCTTTACG TGCGGTACTG CAGCTTTGAG 
GGCTACGAAT ACCTGTTCCA CGTGTACAGG CTCAGTGTGA GTGATCACTC TGTTTTTGCG 
CTGCTCAACA ACACAATGTT CTCCGGGGTA AGTCTTCTGT ATCAGCACCA CGGAGTTAGC 
GTGTCGGAAC ACAGCGTGCT GCGCTTGGTG GGGAACAGTG GCTCAGCTCG CTACGCCATC 
TGTAACGATG GATTGTGGAC TGTTCAGCGG TCAAGCTGGT TGGATTGGCG CGACAACGAC 
GTGGAAGTGG GTGCGATGTT TCACGAATCC GAATCCACTT TTGTGAGCAT TGACGGCAGC 
AGTGTGGTGA CGCTGACGGG CTGCAAGATG GGCTTGACGG GGTTGTCGGG ACCTCTGCTA 
TCGCGTGCTA ACGCTGGCTA CCGGTTCGTT GCTGGGTGCC TGACGGTCGC AGGCCGGGAG 
GTGACGACGG CGGCAGAACT GGTGCTCCAC GGCATCACCA ACGTGACGAC GGTTGCTGCG 
TGCGGGGAGT GTACGAAGGA CGGCGACTGC TTTGCTCCGC TGACGGTGGC GGTCATTGAC 
TGCAAGTGCC AGTGCGCTGC TGGAGGGCAC GGCGATGTGT GTGTCCCGGC GCCTGTGCCT 
GCTGGCCCGC CGCCACCGCC GTCGCCGCCA CTGCTGCCCA TTCCGCCGCC ACCGCCGGTT 
GGTGAGTGCA TCAGCGACAT GGTGTACCCC GAGGTTGCGC AGGCTGTGGG CGGCGGGCTG 
TCGTGGCTGT GCTACCGCAA CGTGACGTTC AGCGGGGGCC GCATGATTCT GACGGTGCTG 
ATTGGGGCGA TGACGGGCGA AGTGGTGAAT GTCACGTTTG ATGGATGCAC GTGGAGGGAC 
GGTGCCGTGC TGGTGCTTTT GGGCAACGTG AACGCCGCTG TGGGGTCGCT GAACATCGTC 
GTCACCGGCA ACACGTTCCG TGACGCACTG CTCAGCCCGG AGGGCGTGTT CCCACCGCAC 
ACGAACATCA CGATCAGCAG GAACCGCTTT ACGGTCACGA GGCTGGTCCT TCGGTCGGGC 
TTGGACATAT GGATCCCGTC CTGCGTTGTG ATGAATGGAC TGGCGATCAG CAACGACTCC 
GCGGTGGTGC TTACTGGCAA TGTGTTTCAG AGCGTGACGG CATCGTCAAG CGCCATTTAC 
GTTGTCGGAT CTGCGCTGAG GGTGTCGTGG CACTCCGTGT TTGCGGTGGT GGGCAACACT 
TTTCATATGG CTGGCGGTGA CAGTACGCTG ATAAATCTTG AAGGGTCTGG CCAATCCTCA 
TCGCTGAGTG TACTGAACAA CTCTGCCGTG GTGATCCGAG GCAATCTTGT GTCGAGGCCG 
GTGAGGTACT TCTTATTGGT AATTTTGGCG TTACGTGTGG AGTCTCTTTC AGCGGTTGTG 
TTTCAGGGCA ACGACATGCA GGGAAGCTTG GCTGTTTTTT TTCCAAGATA CTCCTCCAAC 
ATCTACTACA ACTCCTGGCT GCAGTTGAGC GGCAACCTCT GCCGTGAATC CCCATCGGAA 
GCTTTCCTCT TTTTTAATCC TACGGTGAAT CTCCGCGACT CCACGGTGTC TGTGTCTGGC 
AACCGATTCA TCTCCAGCAC AGGAATGTCG AAGATACTTT GGATATTCAC AGGGCCCCGC 
GATCTCACAA ACGGAGCGAT TGTGGCTGCG TGCAACACCG TGAACGGCGA GGGGGAAGCG 
AAATACAGCA TTCCGTCCGT GTACAATGCC ACCATTTTGA CCTGCAGCGA CCAATGCGCC 
CTCGCGGCAT CGTGCTTCCC TGCGTACACG ACGACGGCGT CGAGTGATGG CTGCACCTGC 
ACGTGCGCTG AGGGCGGCCA CGGCGATGCG TGCCTGCCCG TCGCTGCTCC AGAACCACCG 
AGCACCGACG GCGCCGACTT GTGCGTGCGG GACGTGCGTG TGGATGTGGG GGTCAGCGCC 
GGTCTCGGGA CGTCGGTGGT GTGCTACGTT GGTGTGACCT TTGCGGCGGA TGTCGTGGTG 
GACGTGGACT TGATGTCGGG GAGTGTGCGC AACGTGACGC TGTCGAACTG CACGCTTGTG 
GGCGGAGCGA GCCTGTACGT TGTTGGGTGG CTGTCTGACC CGCCTGCTGG CCAGCGCGCC 
GATGTGTTGA TCAGCGGGCT TGAGTCGCGC TCCGGCGGCG GCGTGGTGGT GGCGAACCGC 
TACCCGCCGG GCTCGCGCGT TACTGTGGTG GACTCTGTGC TGATCGCGGA GAAGCGTGTT 
GCGTACCGCG GCGCCTACGG CCTTGGCGCC GCCTCCGCGT GCCTCGTGCT GCACAGCGTG 
AATCTGACTG GGAGCGTGCT GACCATTGCG CGCACGCACG TGGCTGCGGT GTTCCGCGAC 
GCTGTTGGCG TGTTGTTGGT TGGCGGCGTG GCGCTGTTGT CGCGCGGTGC GCTGTACGTG 
GACGGGCTGT TGGTGCAGAC GGCGCTGGGG CTGTGCGTGT CGGTGGAGGG CGGTGTTGCG 
GCCAGTGGCG GCTCCGTGGT GGCGTTTGTT GACAGCGACT TCCTGCTGTG CAAGCACGCG 
TTGTCTGTGC GTGGGGCTGT GAGCGTGTCG GGCTCCGCTG TGGCGTTTTT GCGGAGCGAA 
TTTGTGTTGA CGGAGGACTA CGCGGTGGCG TTTTATTCAA CGATGAGACT GGCCGACGGG 
TCGATGCTGC TGGCGAAGGG CAACGTGCAC GACGGCGTCT CGAGGGAGAT GCTCTACACC 
GCTGGCGCCG TGACCGCTGC TGGGAGCACG CTGAGCTTTG TGCGCAACCG AGCGCTGCTG 
CCGCGGATGC TGTCGCTGAG CCTGTCGCTC TCGGCTGGGG CGCATTTGAG GGTGGCGTGC 
AACGACGCTG GTGGCCGTGT TCTGTCGACG GCGGAGGAGT ACGCAGCGGC TGGCTTTGGC 
GACGCTGGGA GCATCGACGT TGTTGGGTGC GACGCCTGCG ACAGGGACAC GCACTGCTAT 
GCGCCCGGGA CGGCGTCGGC GAGCATGAGG AACGGTGCGT GCGTGTGCGA TTGCGGCGGC 
GGGTACGGCG AGGCGTGCGT GCCTGTGGGC GCTCCCGCGC TGCCGTCAGC TGCGGGCACT 
GCGTCGAGTG TGTTTCTCCG CGAGGGCGTG ACGGTGCGGT CGGTGTTCGT TGTGCCTGCC 
GGCGCGAGCG AGGTGGCGCT GCGCCACGTT GTGCTGGACG GCGTGAGTCC TGTGCTCTAC 
GTGCCGTGGA TGGCGCGAGA CGGCGTGCGG ATCGTTGTGC AGAACGTGTC GCTGCTGAAC 
GGTGCCGTGC TGTACGTGAT GGGCGGTGGA GCGTTGCGCG GTGCGGTGGC GGCGGGGAGC 
GACGAGAGCG GGCCGGTGGA GCTGTCCGTG TGCGATGTGG AGGCGCTGGA CGGTGCGCTT 
GTGCTGACCG GCACGTATCC CGCGGGGTCC GTGCTGACGG TGGCGGATTC CCTGCTGGTT 
GCCGCGAGGC CGACGCCGCT TGTGTATCTT CCCGGCTCGC AGTCCTCGCC GTACGCACCG 
GTGCTGGTGC TGTCGGGCCT GCGGCTTGTG CGGTCCGTGC TGGTTGTGTC CGGCGTTGCT 
CTCGTGACCG TGGTGACTGG TGGGCGGACG GTGGTGATGG ACGGCGCTGT GCTGGAGCTT 
GTCGGTGGCG GTGTCGCGCT GGACGCGGCT GTGCTCGGTG GCGAATATGC GTTGTACACG 
AGTGCGCGCG TGGTGGCGTC TGGGGGCGCT GTGCTGCGCG TGTCGGGGAG CCAGGTGTAC 
GCCGCGCATG GGTTGGTGTT CGAGAGCGGT GTGGAGGCCA ACGCGTCCGC CGTCGTATTG 
AACGACAACG CCGGTGCGCT GACGGATGGC GCGCTGCTGG TGCTGCGGGG GTCGGCGTCG 
TTTGCGTCCG GGTCGTGGCT GTCGGTGCGC GGCAACAGCA TCAGCGGCAG GCTCCTGTCG 
CTGCCGTCGT ACCCGCGCAG TGCGGACTTT GTGCAGAGCA CGCTGACGCT CCACGGGAAC 
GCCGGCAGCG GGCCCGTCGT GATGGACGGC ACCGTTGCGC TTGGGGACGC CGGCCGAAGG 
TTCGTCGTGG GGTGCCTGAC GCTCAACGGG CGGGCGCTGC AGCCGATGGA CTACAGGTCC 
GCCGGCATCA TCGGGGAATT CCGCCCCGTG ACGTGTGGCG TGTGCAACGC CGACGTGCGC 
TGCTTTGGAG CTGCGACGAG GGCGATGTCT GGGTCGTGCG GCTGCCGCTG TGCTGAGGGC 
GGGTACGGGC GCGACTGCCT GCCGGTGTAC CTACCGTACG TGGACGGGTG CAACCGCACG 
TCTGCCCCGC CGCCGCTGAG CCACACGGCG ACGCTGACGG AGACGCGCAG CCCGACACCA 
ACGTGGACGC CAAGCCTGAG CGTAACGCGC TACAGTCCGA CGCAGTATGG GCCGACGGAG 
ACCCTGCAGA TGACGGAGAC GGTGGCGTTG CCGCCCACGC GGACCTCGAC GGCGTCGGTG 
AGCAGCACGC TGTGGTGGAG CGACGTGGCG TGCCCGACGC TCGCTGTGAC GACGACGGCG 
GCTGGTGGGA GCCTGACGCA GAACGACATT CGCGGCGGTG GGAGCGCCGT CCCGACGCGG 
CTGATGGTGG CGCTGCCGCC GCCGTTTCGA TGGGAACGCG ACCCGCAGCT TGGCACGCAC 
CTGAGCTTTG TGCCGGTGTC GACGGCGCAG CCGCGCGGCT TCGGTGGTCC GTGGGGAGCG 
ATGCTGAGCA ACGCGACGTG GGTGCGCAAC GCGACGAATC CGTCCACCGT CCTGGAGCTG 
GCTGTGCCCG TGCACCGCGG TTACTTCATT GTTGCCGACG AGACGATATT CATTCGCTGC 
GACGCTGCGG CGGTGTTTGG CGGCTGCAGG GGTGTGCTGT TTGGGTCCTT CACGATCAGG 
TCGGACACGC TGCCCGCCGC TGCCAGCGCC CTCTCGGCGA TCACCGGCGT TGTTGCGGGT 
GCGACGGCTG TTGCCGTGGT GGTGACCGGC GGGCTGGGCT CCATCCTGGA GATGCAGGCG 
CTCGGCGTGT TGGCGAGGAT GCCATGCGCC TCGGCGCAGG AGCGTGCGAG CACCGTTGCG 
CTGCCGTACT TCCTGTCGGT GTTCGCTGCC CTTGACCCGC TGTGGATGGT GGTGGGCAAC 
GCGCTGCTGG CCGCAGTGTT CGGGTGCGTG CACTACGGTG TGACGGCGGC CTTCCAGCGG 
TGGCGCGGCG TGGACGCAGC GAGTGCGTGG GCTGCGATGC GCTTCCCGAG CCTGACGTAC 
GTTGTGGCGC ACGCGATGCA TCTTGGCATC TTCTTTGGCT CCGTGTTTGC ACTGGCGATG 
CCCGACGCCC GCGTGCAGCA CTACGTGATT GGTGTCGTTG GCGTGCTGTA CGGAGTGGCG 
TTTCCTGCTG GCGTGTGCTA CTTGATTGCC CGCCACGTGG GCGCGAGCTT CACGAGGTAC 
TGGCAGTTTT CGAGGAAGCC GCTGCACGAG CGCCTGCTGT ACCCCGTCGG GTATTGGCAC 
CCCGCGGCGC AGCAGCGGAT GTACGGCGGC ATGCTGACGA ACATGAAGGG CAGCCACGTG 
TACTGGTGCG TGTTCCAGCT GTCGGTGCTG TGTGTGGTGG GCCTGATTGC GGCTGTGCAC 
CCGCCCGTGG GAGGCTGCCA CGTCCAGTAC TTCTGCATGG CGACTGTGCT GCTTGCGGGT 
GCGGGCGTGG TTGCCTTCAC GAACATGATG CGCTCGGCCT TCCTGACGGT GATGCGCACG 
GCGAGCTTTG TGCTTCTCGC GGCGCTGTGC CTGGTGAGCG CGGCCAACCA TCTTGCGCCG 
AGCGACGGCG GAGCGAGGGC GTACGCGGCG ATCGTGCTGC TGCTGACTGC TGTGCTGCTT 
GCGGTCACTG TGTACGGCGT CGTTGTGTGG TACGCGGAGG ACCGCCACTG GCAGGAGCTG 
CGCGAGCCGC GGCGTTGTGG GCTGGAGGCA CTGCTACGCG ATGACGAGGA GAGCGACGAG 
GACGCGCAGA AGCCTCACGA CATGACGTCA TCGTCGTACG CCTCAGGCAC CACCGTTGCG 
TCGTACCGAC CACCCGCACC GCCGCTGCAG CCCATGGCCG GTGACATTCG CTTAGACGCT 
CTGAGCCTAC TGGACCGTGC ATCCAGTGCG GGCCACAAAA TTGGTCACGC TCTTCCGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_816220.1
CDS	1..10380
Translation	MWSAVGACPRSRHRVRRRAIAAVRVALLVALALVAATAFMPAAHAVVLRLRGGTVDRAITVGHAVDTVLMDGVYVTNGVAVVFDVPAMLPGALRIELRNCVCDGGAQIYVRGYSGEPASDRSLEVSVSRLSGGYCSLVFVHSLPARTNVTVRDSTIVTAGPMRYSQLSGLMDAVASPLVLHATSLLQTQLRVSNTVLRYLQAGGSAVHVGGGVDLLSSAVVLDGVLLEASGDSTAFALHVASSSRLSLRSHSVFSVTNMSVVSSGGGFVLGERLAVSDSVLRFVGVEGSVASSLVRCDGGTVGAGGWLELHDVWAVGEASSVASLSGVTLSGGTVSIARCAATGATLVSGLTITSGVVSVQCNRAGGRVLQSSGDYRMAGLPSVSVVPCDGCAAALACFDALTASFSDCVCGCRAGGVGEACLPFDVPPARSGGGGGTARDCVSGVTLMESVTVGGGRATACFDSVVFRGPITVAVDLRLMDAFADALNVTLRHCVLAGGAQLRIGGLSEITAHLMPHALVNMTNVTSLEGTIVLHGAMPPNSSVLLANSTLRATVGGSRYVPTTPGHERFRYGPALVLDGVRLLSTRFVMTRSTLVCGGSSCAAILVEHSLCAKLSSVFYMDNCAVKSRKHVIYSLASDLRVGGDSVFSIQNSSWSAPSTEYYKGACVFRGVVVAGGSVLQIVSSTFRLGFAMLMAATLTVTGGSWLVHRNNEFHSAYVVYVANENGVAFCDRSVWSILDNNFTYGSYSSTIAHMTTNWSPPSDTHPIIYGMCNEARGSPVTDYGEELNIVAPVTVLDCGACTMNAVCFAARTSSISDCECVCAAGGYGDTCLPAAVPDGLGPLPLPDAKDTEVRCVHGGSISSVDYPDPGVRGLCLVNVTFTAPIVLDLSSFDVPQQTLNVTLLQCVLMGLSIKGSGARVHVSVTSSLLDSGALEFEGDFGASSQILVVGSTIVATSGHAIYFVKFTLSANMTLLLLDNYIEANRHAVYFIDGVVVDGGRIIVKGNTLRATEVDDGEESSVCVTSVDVRNGGYFDVENNTMSSVNGVIFWGDNTVRSAGLLRVADCTFVGGTEIFDPALLHLSGSATLEGGAQWRLEGNSVVAASVLIISYSWHKIQLSGSGTTVVLAHNRQVNDSYPFADLALPSMVKKPPARLVVGCNLQGDEEASYDYVFPEGVVVFRCGTCYDDAACYMPGTELVDRGSCSCSCKDGWHGASCLPFEVPDTAVPPLPERAVDGDASCVVNQTLTDLVLNMWKIHHCYVDVTFSGVGAVLTFSLNSMPLHLPINITFTGCTFRDGAVLKFVGGAEAVESTGVLIRVSQTVMRSSVVVFALAFPQHCDIAVTEVDAVQSSEVHLPDARRNKLSVLLLMNVVLSASSLLVSNVKAHALRYDAFGLYSTGTLTLVGGSSLYVRYCSFEGYEYLFHVYRLSVSDHSVFALLNNTMFSGVSLLYQHHGVSVSEHSVLRLVGNSGSARYAICNDGLWTVQRSSWLDWRDNDVEVGAMFHESESTFVSIDGSSVVTLTGCKMGLTGLSGPLLSRANAGYRFVAGCLTVAGREVTTAAELVLHGITNVTTVAACGECTKDGDCFAPLTVAVIDCKCQCAAGGHGDVCVPAPVPAGPPPPPSPPLLPIPPPPPVGECISDMVYPEVAQAVGGGLSWLCYRNVTFSGGRMILTVLIGAMTGEVVNVTFDGCTWRDGAVLVLLGNVNAAVGSLNIVVTGNTFRDALLSPEGVFPPHTNITISRNRFTVTRLVLRSGLDIWIPSCVVMNGLAISNDSAVVLTGNVFQSVTASSSAIYVVGSALRVSWHSVFAVVGNTFHMAGGDSTLINLEGSGQSSSLSVLNNSAVVIRGNLVSRPVRYFLLVILALRVESLSAVVFQGNDMQGSLAVFFPRYSSNIYYNSWLQLSGNLCRESPSEAFLFFNPTVNLRDSTVSVSGNRFISSTGMSKILWIFTGPRDLTNGAIVAACNTVNGEGEAKYSIPSVYNATILTCSDQCALAASCFPAYTTTASSDGCTCTCAEGGHGDACLPVAAPEPPSTDGADLCVRDVRVDVGVSAGLGTSVVCYVGVTFAADVVVDVDLMSGSVRNVTLSNCTLVGGASLYVVGWLSDPPAGQRADVLISGLESRSGGGVVVANRYPPGSRVTVVDSVLIAEKRVAYRGAYGLGAASACLVLHSVNLTGSVLTIARTHVAAVFRDAVGVLLVGGVALLSRGALYVDGLLVQTALGLCVSVEGGVAASGGSVVAFVDSDFLLCKHALSVRGAVSVSGSAVAFLRSEFVLTEDYAVAFYSTMRLADGSMLLAKGNVHDGVSREMLYTAGAVTAAGSTLSFVRNRALLPRMLSLSLSLSAGAHLRVACNDAGGRVLSTAEEYAAAGFGDAGSIDVVGCDACDRDTHCYAPGTASASMRNGACVCDCGGGYGEACVPVGAPALPSAAGTASSVFLREGVTVRSVFVVPAGASEVALRHVVLDGVSPVLYVPWMARDGVRIVVQNVSLLNGAVLYVMGGGALRGAVAAGSDESGPVELSVCDVEALDGALVLTGTYPAGSVLTVADSLLVAARPTPLVYLPGSQSSPYAPVLVLSGLRLVRSVLVVSGVALVTVVTGGRTVVMDGAVLELVGGGVALDAAVLGGEYALYTSARVVASGGAVLRVSGSQVYAAHGLVFESGVEANASAVVLNDNAGALTDGALLVLRGSASFASGSWLSVRGNSISGRLLSLPSYPRSADFVQSTLTLHGNAGSGPVVMDGTVALGDAGRRFVVGCLTLNGRALQPMDYRSAGIIGEFRPVTCGVCNADVRCFGAATRAMSGSCGCRCAEGGYGRDCLPVYLPYVDGCNRTSAPPPLSHTATLTETRSPTPTWTPSLSVTRYSPTQYGPTETLQMTETVALPPTRTSTASVSSTLWWSDVACPTLAVTTTAAGGSLTQNDIRGGGSAVPTRLMVALPPPFRWERDPQLGTHLSFVPVSTAQPRGFGGPWGAMLSNATWVRNATNPSTVLELAVPVHRGYFIVADETIFIRCDAAAVFGGCRGVLFGSFTIRSDTLPAAASALSAITGVVAGATAVAVVVTGGLGSILEMQALGVLARMPCASAQERASTVALPYFLSVFAALDPLWMVVGNALLAAVFGCVHYGVTAAFQRWRGVDAASAWAAMRFPSLTYVVAHAMHLGIFFGSVFALAMPDARVQHYVIGVVGVLYGVAFPAGVCYLIARHVGASFTRYWQFSRKPLHERLLYPVGYWHPAAQQRMYGGMLTNMKGSHVYWCVFQLSVLCVVGLIAAVHPPVGGCHVQYFCMATVLLAGAGVVAFTNMMRSAFLTVMRTASFVLLAALCLVSAANHLAPSDGGARAYAAIVLLLTAVLLAVTVYGVVVWYAEDRHWQELREPRRCGLEALLRDDEESDEDAQKPHDMTSSSYASGTTVASYRPPAPPLQPMAGDIRLDALSLLDRASSAGHKIGHALP

Target ORF information:

RefSeq Version	XM_811127.1
Organism	Trypanosoma cruzi strain CL Brener
Definition	Trypanosoma cruzi strain CL Brener dispersed gene family protein 1 (DGF-1) partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_811127.1

ORF Insert Sequence:

ATGTGGTCTG CGGTGGGTGC GTGTCCGCGC AGCCGGCACC GTGTGCGGCG CAGGGCCATT 
GCGGCTGTGC GTGTGGCACT GCTTGTGGCG TTGGCGCTGG TTGCGGCGAC GGCGTTTATG 
CCCGCGGCGC ATGCGGTGGT GTTGCGACTG CGGGGCGGCA CGGTGGACAG AGCCATCACG 
GTTGGCCACG CCGTGGACAC GGTGCTGATG GACGGCGTGT ACGTCACGAA CGGCGTGGCC 
GTGGTGTTCG ACGTGCCGGC GATGCTTCCC GGCGCGCTGC GCATCGAACT GAGGAACTGC 
GTGTGCGACG GCGGTGCGCA GATCTACGTG CGGGGCTACA GCGGTGAGCC GGCGAGTGAC 
CGGAGCCTGG AGGTGAGCGT GAGCAGGCTG TCCGGCGGCT ACTGCTCGCT TGTGTTTGTG 
CACAGCCTGC CGGCACGCAC GAACGTGACG GTCCGTGACT CGACGATTGT GACGGCGGGG 
CCGATGCGCT ACTCCCAGCT GAGCGGGCTG ATGGACGCGG TGGCGTCGCC GCTTGTGCTG 
CACGCGACGT CGCTGTTGCA GACGCAGCTG CGTGTGAGCA ACACTGTGCT GAGGTATTTA 
CAGGCGGGCG GGTCGGCGGT GCACGTTGGC GGCGGTGTGG ACCTGCTGTC GAGCGCGGTG 
GTGCTTGACG GCGTGTTGCT GGAGGCGTCG GGCGATTCGA CCGCGTTCGC GTTGCATGTG 
GCGTCCTCGT CGCGTCTCAG TCTGCGGAGC CACTCGGTGT TCTCCGTGAC GAACATGTCG 
GTTGTGAGCA GTGGCGGCGG CTTTGTGCTT GGCGAGCGCC TCGCGGTGTC GGACTCGGTG 
CTGCGCTTCG TGGGCGTCGA GGGGTCTGTT GCGTCGTCGC TGGTGCGCTG CGACGGTGGG 
ACGGTGGGTG CTGGCGGGTG GCTGGAGCTG CACGACGTGT GGGCGGTGGG CGAGGCCTCG 
TCTGTGGCGT CGCTGTCGGG GGTGACGTTG AGTGGCGGCA CCGTGTCTAT TGCGCGCTGC 
GCTGCCACTG GCGCGACGCT TGTCTCCGGG CTGACGATCA CGAGCGGCGT CGTGTCGGTG 
CAATGCAACC GTGCCGGCGG CCGGGTGCTG CAGAGCAGCG GCGACTACCG CATGGCCGGC 
CTGCCCTCCG TGAGCGTGGT TCCGTGCGAC GGCTGTGCGG CTGCGCTGGC GTGCTTTGAT 
GCGCTGACGG CGTCGTTCTC CGACTGCGTG TGCGGCTGCC GTGCCGGCGG CGTGGGCGAG 
GCGTGCCTGC CGTTCGACGT GCCGCCTGCG AGGTCCGGCG GTGGTGGCGG TACTGCGCGG 
GACTGCGTGA GTGGCGTGAC GCTGATGGAG TCGGTGACGG TTGGCGGCGG GCGGGCGACG 
GCGTGCTTCG ACTCGGTGGT GTTCAGAGGG CCAATCACTG TGGCGGTGGA CCTGCGCTTG 
ATGGACGCGT TCGCTGACGC GCTGAACGTG ACGCTGCGCC ACTGCGTGCT TGCAGGTGGC 
GCGCAGCTGC GGATTGGTGG CCTCAGCGAG ATCACGGCGC ACCTGATGCC CCATGCCCTC 
GTCAACATGA CGAATGTGAC GTCGCTGGAG GGCACGATCG TGCTGCATGG CGCGATGCCG 
CCGAACTCGA GTGTGCTGCT GGCGAACTCG ACGCTGCGCG CGACGGTTGG CGGGTCGCGG 
TACGTGCCGA CGACCCCGGG CCACGAGAGA TTTCGGTACG GCCCCGCGCT TGTCCTGGAC 
GGTGTCCGGC TTCTGTCGAC GCGGTTTGTC ATGACGCGGT CGACGCTGGT GTGTGGCGGG 
AGCTCGTGCG CTGCTATCCT CGTGGAGCAC AGCCTTTGCG CGAAGCTGTC CAGCGTCTTC 
TACATGGACA ACTGCGCTGT CAAGTCGCGG AAGCATGTGA TTTACTCTCT TGCGTCGGAC 
CTGCGTGTTG GCGGCGACTC CGTGTTCTCC ATACAGAACA GCTCGTGGAG TGCGCCGAGC 
ACCGAGTACT ACAAAGGTGC GTGTGTGTTT AGGGGTGTTG TTGTGGCTGG CGGCAGCGTG 
CTGCAGATTG TGTCCAGCAC TTTCCGTCTC GGCTTTGCCA TGCTCATGGC AGCCACGCTG 
ACCGTGACTG GCGGCAGCTG GCTGGTGCAC CGCAACAACG AGTTCCACAG CGCCTACGTT 
GTGTACGTGG CCAACGAGAA CGGCGTGGCG TTTTGCGATC GGAGTGTGTG GTCGATACTT 
GACAACAACT TCACGTACGG CTCGTACTCG TCAACCATTG CGCATATGAC AACCAATTGG 
TCACCACCAT CCGACACGCA CCCGATCATC TACGGCATGT GCAACGAGGC AAGGGGCTCT 
CCTGTGACGG ATTACGGAGA AGAGCTCAAT ATTGTGGCGC CCGTGACGGT GCTGGACTGT 
GGTGCGTGCA CGATGAACGC CGTGTGCTTT GCTGCGAGGA CTAGTAGCAT CAGCGACTGT 
GAGTGTGTGT GTGCTGCTGG CGGCTACGGT GACACGTGCC TGCCGGCTGC GGTTCCGGAC 
GGCCTTGGGC CGCTCCCGCT CCCCGATGCG AAGGACACGG AGGTCCGCTG CGTGCACGGT 
GGCAGCATCA GCTCCGTGGA CTATCCTGAT CCCGGTGTGC GTGGTCTGTG CCTCGTCAAC 
GTGACCTTCA CCGCCCCGAT TGTGCTGGAC CTGTCGAGCT TCGACGTACC GCAGCAGACA 
CTCAACGTCA CGCTGCTGCA GTGCGTCCTC ATGGGCCTGT CGATCAAGGG GAGTGGTGCG 
CGTGTGCACG TGAGCGTGAC ATCCTCGTTG TTGGATTCTG GCGCATTGGA GTTTGAGGGT 
GATTTTGGCG CGAGCTCCCA GATACTGGTG GTGGGCAGTA CGATTGTGGC GACATCGGGC 
CACGCCATTT ACTTTGTGAA GTTTACTCTC AGTGCGAATA TGACGCTGCT GCTACTCGAC 
AACTACATTG AGGCGAATAG GCACGCAGTT TATTTTATTG ATGGTGTTGT TGTTGATGGT 
GGCAGGATCA TTGTGAAGGG AAATACTTTG AGGGCAACGG AAGTCGATGA CGGTGAGGAA 
TCATCTGTTT GCGTTACTTC TGTTGATGTG AGGAACGGCG GCTACTTTGA CGTGGAGAAC 
AACACGATGA GCTCGGTCAA TGGCGTTATT TTTTGGGGGG ATAATACCGT GAGGTCCGCG 
GGGCTGCTGC GAGTGGCGGA CTGCACCTTT GTTGGCGGGA CAGAAATTTT TGACCCCGCG 
CTGTTGCATT TGTCTGGCTC GGCGACACTT GAGGGAGGTG CGCAGTGGCG TTTGGAGGGC 
AACAGCGTGG TTGCCGCATC AGTATTAATT ATTTCGTATT CTTGGCACAA AATTCAGCTG 
TCGGGCAGCG GCACCACCGT GGTGCTTGCA CACAACCGTC AGGTGAACGA TAGTTATCCC 
TTTGCTGATC TGGCTCTGCC GAGCATGGTC AAGAAGCCAC CCGCACGGTT GGTCGTTGGG 
TGCAACCTGC AGGGCGATGA GGAGGCGTCG TACGACTATG TGTTTCCTGA GGGCGTGGTG 
GTGTTCAGGT GTGGCACATG CTACGACGAC GCGGCGTGCT ACATGCCCGG GACGGAGTTG 
GTGGACCGCG GCTCGTGCTC GTGCAGCTGC AAGGACGGAT GGCATGGCGC GTCGTGTCTT 
CCCTTCGAGG TGCCCGACAC GGCTGTGCCG CCCTTGCCGG AAAGAGCCGT GGACGGCGAC 
GCGAGCTGCG TGGTGAACCA GACGCTGACG GACCTCGTGC TGAACATGTG GAAGATACAC 
CACTGTTACG TGGATGTGAC ATTCAGCGGC GTGGGTGCAG TGCTGACGTT CTCCCTCAAC 
AGTATGCCGC TGCATCTCCC CATCAACATC ACGTTTACCG GGTGCACATT CCGCGACGGT 
GCCGTGCTGA AGTTTGTTGG AGGTGCTGAG GCTGTTGAGT CAACCGGCGT CCTGATCCGC 
GTGAGCCAGA CGGTGATGCG TTCCTCCGTT GTTGTGTTTG CACTTGCCTT CCCGCAGCAC 
TGTGACATCG CCGTTACGGA GGTTGATGCG GTGCAGTCCT CCGAGGTCCA TCTGCCGGAC 
GCCAGGAGGA ACAAGCTTAG CGTACTTTTA CTGATGAATG TTGTGCTGAG TGCGTCCTCG 
CTGCTGGTGA GCAACGTCAA GGCGCATGCA TTGAGGTATG ATGCGTTTGG TTTGTACTCG 
ACCGGGACGC TGACGCTGGT GGGTGGCAGC TCTCTTTACG TGCGGTACTG CAGCTTTGAG 
GGCTACGAAT ACCTGTTCCA CGTGTACAGG CTCAGTGTGA GTGATCACTC TGTTTTTGCG 
CTGCTCAACA ACACAATGTT CTCCGGGGTA AGTCTTCTGT ATCAGCACCA CGGAGTTAGC 
GTGTCGGAAC ACAGCGTGCT GCGCTTGGTG GGGAACAGTG GCTCAGCTCG CTACGCCATC 
TGTAACGATG GATTGTGGAC TGTTCAGCGG TCAAGCTGGT TGGATTGGCG CGACAACGAC 
GTGGAAGTGG GTGCGATGTT TCACGAATCC GAATCCACTT TTGTGAGCAT TGACGGCAGC 
AGTGTGGTGA CGCTGACGGG CTGCAAGATG GGCTTGACGG GGTTGTCGGG ACCTCTGCTA 
TCGCGTGCTA ACGCTGGCTA CCGGTTCGTT GCTGGGTGCC TGACGGTCGC AGGCCGGGAG 
GTGACGACGG CGGCAGAACT GGTGCTCCAC GGCATCACCA ACGTGACGAC GGTTGCTGCG 
TGCGGGGAGT GTACGAAGGA CGGCGACTGC TTTGCTCCGC TGACGGTGGC GGTCATTGAC 
TGCAAGTGCC AGTGCGCTGC TGGAGGGCAC GGCGATGTGT GTGTCCCGGC GCCTGTGCCT 
GCTGGCCCGC CGCCACCGCC GTCGCCGCCA CTGCTGCCCA TTCCGCCGCC ACCGCCGGTT 
GGTGAGTGCA TCAGCGACAT GGTGTACCCC GAGGTTGCGC AGGCTGTGGG CGGCGGGCTG 
TCGTGGCTGT GCTACCGCAA CGTGACGTTC AGCGGGGGCC GCATGATTCT GACGGTGCTG 
ATTGGGGCGA TGACGGGCGA AGTGGTGAAT GTCACGTTTG ATGGATGCAC GTGGAGGGAC 
GGTGCCGTGC TGGTGCTTTT GGGCAACGTG AACGCCGCTG TGGGGTCGCT GAACATCGTC 
GTCACCGGCA ACACGTTCCG TGACGCACTG CTCAGCCCGG AGGGCGTGTT CCCACCGCAC 
ACGAACATCA CGATCAGCAG GAACCGCTTT ACGGTCACGA GGCTGGTCCT TCGGTCGGGC 
TTGGACATAT GGATCCCGTC CTGCGTTGTG ATGAATGGAC TGGCGATCAG CAACGACTCC 
GCGGTGGTGC TTACTGGCAA TGTGTTTCAG AGCGTGACGG CATCGTCAAG CGCCATTTAC 
GTTGTCGGAT CTGCGCTGAG GGTGTCGTGG CACTCCGTGT TTGCGGTGGT GGGCAACACT 
TTTCATATGG CTGGCGGTGA CAGTACGCTG ATAAATCTTG AAGGGTCTGG CCAATCCTCA 
TCGCTGAGTG TACTGAACAA CTCTGCCGTG GTGATCCGAG GCAATCTTGT GTCGAGGCCG 
GTGAGGTACT TCTTATTGGT AATTTTGGCG TTACGTGTGG AGTCTCTTTC AGCGGTTGTG 
TTTCAGGGCA ACGACATGCA GGGAAGCTTG GCTGTTTTTT TTCCAAGATA CTCCTCCAAC 
ATCTACTACA ACTCCTGGCT GCAGTTGAGC GGCAACCTCT GCCGTGAATC CCCATCGGAA 
GCTTTCCTCT TTTTTAATCC TACGGTGAAT CTCCGCGACT CCACGGTGTC TGTGTCTGGC 
AACCGATTCA TCTCCAGCAC AGGAATGTCG AAGATACTTT GGATATTCAC AGGGCCCCGC 
GATCTCACAA ACGGAGCGAT TGTGGCTGCG TGCAACACCG TGAACGGCGA GGGGGAAGCG 
AAATACAGCA TTCCGTCCGT GTACAATGCC ACCATTTTGA CCTGCAGCGA CCAATGCGCC 
CTCGCGGCAT CGTGCTTCCC TGCGTACACG ACGACGGCGT CGAGTGATGG CTGCACCTGC 
ACGTGCGCTG AGGGCGGCCA CGGCGATGCG TGCCTGCCCG TCGCTGCTCC AGAACCACCG 
AGCACCGACG GCGCCGACTT GTGCGTGCGG GACGTGCGTG TGGATGTGGG GGTCAGCGCC 
GGTCTCGGGA CGTCGGTGGT GTGCTACGTT GGTGTGACCT TTGCGGCGGA TGTCGTGGTG 
GACGTGGACT TGATGTCGGG GAGTGTGCGC AACGTGACGC TGTCGAACTG CACGCTTGTG 
GGCGGAGCGA GCCTGTACGT TGTTGGGTGG CTGTCTGACC CGCCTGCTGG CCAGCGCGCC 
GATGTGTTGA TCAGCGGGCT TGAGTCGCGC TCCGGCGGCG GCGTGGTGGT GGCGAACCGC 
TACCCGCCGG GCTCGCGCGT TACTGTGGTG GACTCTGTGC TGATCGCGGA GAAGCGTGTT 
GCGTACCGCG GCGCCTACGG CCTTGGCGCC GCCTCCGCGT GCCTCGTGCT GCACAGCGTG 
AATCTGACTG GGAGCGTGCT GACCATTGCG CGCACGCACG TGGCTGCGGT GTTCCGCGAC 
GCTGTTGGCG TGTTGTTGGT TGGCGGCGTG GCGCTGTTGT CGCGCGGTGC GCTGTACGTG 
GACGGGCTGT TGGTGCAGAC GGCGCTGGGG CTGTGCGTGT CGGTGGAGGG CGGTGTTGCG 
GCCAGTGGCG GCTCCGTGGT GGCGTTTGTT GACAGCGACT TCCTGCTGTG CAAGCACGCG 
TTGTCTGTGC GTGGGGCTGT GAGCGTGTCG GGCTCCGCTG TGGCGTTTTT GCGGAGCGAA 
TTTGTGTTGA CGGAGGACTA CGCGGTGGCG TTTTATTCAA CGATGAGACT GGCCGACGGG 
TCGATGCTGC TGGCGAAGGG CAACGTGCAC GACGGCGTCT CGAGGGAGAT GCTCTACACC 
GCTGGCGCCG TGACCGCTGC TGGGAGCACG CTGAGCTTTG TGCGCAACCG AGCGCTGCTG 
CCGCGGATGC TGTCGCTGAG CCTGTCGCTC TCGGCTGGGG CGCATTTGAG GGTGGCGTGC 
AACGACGCTG GTGGCCGTGT TCTGTCGACG GCGGAGGAGT ACGCAGCGGC TGGCTTTGGC 
GACGCTGGGA GCATCGACGT TGTTGGGTGC GACGCCTGCG ACAGGGACAC GCACTGCTAT 
GCGCCCGGGA CGGCGTCGGC GAGCATGAGG AACGGTGCGT GCGTGTGCGA TTGCGGCGGC 
GGGTACGGCG AGGCGTGCGT GCCTGTGGGC GCTCCCGCGC TGCCGTCAGC TGCGGGCACT 
GCGTCGAGTG TGTTTCTCCG CGAGGGCGTG ACGGTGCGGT CGGTGTTCGT TGTGCCTGCC 
GGCGCGAGCG AGGTGGCGCT GCGCCACGTT GTGCTGGACG GCGTGAGTCC TGTGCTCTAC 
GTGCCGTGGA TGGCGCGAGA CGGCGTGCGG ATCGTTGTGC AGAACGTGTC GCTGCTGAAC 
GGTGCCGTGC TGTACGTGAT GGGCGGTGGA GCGTTGCGCG GTGCGGTGGC GGCGGGGAGC 
GACGAGAGCG GGCCGGTGGA GCTGTCCGTG TGCGATGTGG AGGCGCTGGA CGGTGCGCTT 
GTGCTGACCG GCACGTATCC CGCGGGGTCC GTGCTGACGG TGGCGGATTC CCTGCTGGTT 
GCCGCGAGGC CGACGCCGCT TGTGTATCTT CCCGGCTCGC AGTCCTCGCC GTACGCACCG 
GTGCTGGTGC TGTCGGGCCT GCGGCTTGTG CGGTCCGTGC TGGTTGTGTC CGGCGTTGCT 
CTCGTGACCG TGGTGACTGG TGGGCGGACG GTGGTGATGG ACGGCGCTGT GCTGGAGCTT 
GTCGGTGGCG GTGTCGCGCT GGACGCGGCT GTGCTCGGTG GCGAATATGC GTTGTACACG 
AGTGCGCGCG TGGTGGCGTC TGGGGGCGCT GTGCTGCGCG TGTCGGGGAG CCAGGTGTAC 
GCCGCGCATG GGTTGGTGTT CGAGAGCGGT GTGGAGGCCA ACGCGTCCGC CGTCGTATTG 
AACGACAACG CCGGTGCGCT GACGGATGGC GCGCTGCTGG TGCTGCGGGG GTCGGCGTCG 
TTTGCGTCCG GGTCGTGGCT GTCGGTGCGC GGCAACAGCA TCAGCGGCAG GCTCCTGTCG 
CTGCCGTCGT ACCCGCGCAG TGCGGACTTT GTGCAGAGCA CGCTGACGCT CCACGGGAAC 
GCCGGCAGCG GGCCCGTCGT GATGGACGGC ACCGTTGCGC TTGGGGACGC CGGCCGAAGG 
TTCGTCGTGG GGTGCCTGAC GCTCAACGGG CGGGCGCTGC AGCCGATGGA CTACAGGTCC 
GCCGGCATCA TCGGGGAATT CCGCCCCGTG ACGTGTGGCG TGTGCAACGC CGACGTGCGC 
TGCTTTGGAG CTGCGACGAG GGCGATGTCT GGGTCGTGCG GCTGCCGCTG TGCTGAGGGC 
GGGTACGGGC GCGACTGCCT GCCGGTGTAC CTACCGTACG TGGACGGGTG CAACCGCACG 
TCTGCCCCGC CGCCGCTGAG CCACACGGCG ACGCTGACGG AGACGCGCAG CCCGACACCA 
ACGTGGACGC CAAGCCTGAG CGTAACGCGC TACAGTCCGA CGCAGTATGG GCCGACGGAG 
ACCCTGCAGA TGACGGAGAC GGTGGCGTTG CCGCCCACGC GGACCTCGAC GGCGTCGGTG 
AGCAGCACGC TGTGGTGGAG CGACGTGGCG TGCCCGACGC TCGCTGTGAC GACGACGGCG 
GCTGGTGGGA GCCTGACGCA GAACGACATT CGCGGCGGTG GGAGCGCCGT CCCGACGCGG 
CTGATGGTGG CGCTGCCGCC GCCGTTTCGA TGGGAACGCG ACCCGCAGCT TGGCACGCAC 
CTGAGCTTTG TGCCGGTGTC GACGGCGCAG CCGCGCGGCT TCGGTGGTCC GTGGGGAGCG 
ATGCTGAGCA ACGCGACGTG GGTGCGCAAC GCGACGAATC CGTCCACCGT CCTGGAGCTG 
GCTGTGCCCG TGCACCGCGG TTACTTCATT GTTGCCGACG AGACGATATT CATTCGCTGC 
GACGCTGCGG CGGTGTTTGG CGGCTGCAGG GGTGTGCTGT TTGGGTCCTT CACGATCAGG 
TCGGACACGC TGCCCGCCGC TGCCAGCGCC CTCTCGGCGA TCACCGGCGT TGTTGCGGGT 
GCGACGGCTG TTGCCGTGGT GGTGACCGGC GGGCTGGGCT CCATCCTGGA GATGCAGGCG 
CTCGGCGTGT TGGCGAGGAT GCCATGCGCC TCGGCGCAGG AGCGTGCGAG CACCGTTGCG 
CTGCCGTACT TCCTGTCGGT GTTCGCTGCC CTTGACCCGC TGTGGATGGT GGTGGGCAAC 
GCGCTGCTGG CCGCAGTGTT CGGGTGCGTG CACTACGGTG TGACGGCGGC CTTCCAGCGG 
TGGCGCGGCG TGGACGCAGC GAGTGCGTGG GCTGCGATGC GCTTCCCGAG CCTGACGTAC 
GTTGTGGCGC ACGCGATGCA TCTTGGCATC TTCTTTGGCT CCGTGTTTGC ACTGGCGATG 
CCCGACGCCC GCGTGCAGCA CTACGTGATT GGTGTCGTTG GCGTGCTGTA CGGAGTGGCG 
TTTCCTGCTG GCGTGTGCTA CTTGATTGCC CGCCACGTGG GCGCGAGCTT CACGAGGTAC 
TGGCAGTTTT CGAGGAAGCC GCTGCACGAG CGCCTGCTGT ACCCCGTCGG GTATTGGCAC 
CCCGCGGCGC AGCAGCGGAT GTACGGCGGC ATGCTGACGA ACATGAAGGG CAGCCACGTG 
TACTGGTGCG TGTTCCAGCT GTCGGTGCTG TGTGTGGTGG GCCTGATTGC GGCTGTGCAC 
CCGCCCGTGG GAGGCTGCCA CGTCCAGTAC TTCTGCATGG CGACTGTGCT GCTTGCGGGT 
GCGGGCGTGG TTGCCTTCAC GAACATGATG CGCTCGGCCT TCCTGACGGT GATGCGCACG 
GCGAGCTTTG TGCTTCTCGC GGCGCTGTGC CTGGTGAGCG CGGCCAACCA TCTTGCGCCG 
AGCGACGGCG GAGCGAGGGC GTACGCGGCG ATCGTGCTGC TGCTGACTGC TGTGCTGCTT 
GCGGTCACTG TGTACGGCGT CGTTGTGTGG TACGCGGAGG ACCGCCACTG GCAGGAGCTG 
CGCGAGCCGC GGCGTTGTGG GCTGGAGGCA CTGCTACGCG ATGACGAGGA GAGCGACGAG 
GACGCGCAGA AGCCTCACGA CATGACGTCA TCGTCGTACG CCTCAGGCAC CACCGTTGCG 
TCGTACCGAC CACCCGCACC GCCGCTGCAG CCCATGGCCG GTGACATTCG CTTAGACGCT 
CTGAGCCTAC TGGACCGTGC ATCCAGTGCG GGCCACAAAA TTGGTCACGC TCTTCCGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The genome sequence of Trypanosoma cruzi, etiologic agent of Chagas disease.
Science (New York, N.Y.)309(5733)409-15(2005 Jul)
El-Sayed NM,Myler PJ,Bartholomeu DC,Nilsson D,Aggarwal G,Tran AN,Ghedin E,Worthey EA,Delcher AL,Blandin G,Westenberger SJ,Caler E,Cerqueira GC,Branche C,Haas B,Anupama A,Arner E,Aslund L,Attipoe P,Bontempi E,Bringaud F,Burton P,Cadag E,Campbell DA,Carrington M,Crabtree J,Darban H,da Silveira JF,de Jong P,Edwards K,Englund PT,Fazelina G,Feldblyum T,Ferella M,Frasch AC,Gull K,Horn D,Hou L,Huang Y,Kindlund E,Klingbeil M,Kluge S,Koo H,Lacerda D,Levin MJ,Lorenzi H,Louie T,Machado CR,McCulloch R,McKenna A,Mizuno Y,Mottram JC,Nelson S,Ochaya S,Osoegawa K,Pai G,Parsons M,Pentony M,Pettersson U,Pop M,Ramirez JL,Rinta J,Robertson L,Salzberg SL,Sanchez DO,Seyler A,Sharma R,Shetty J,Simpson AJ,Sisk E,Tammi MT,Tarleton R,Teixeira S,Van Aken S,Vogt C,Ward PN,Wickstead B,Wortman J,White O,Fraser CM,Stuart KD,Andersson B

Comparative genomics of trypanosomatid parasitic protozoa.
Science (New York, N.Y.)309(5733)404-9(2005 Jul)
El-Sayed NM,Myler PJ,Blandin G,Berriman M,Crabtree J,Aggarwal G,Caler E,Renauld H,Worthey EA,Hertz-Fowler C,Ghedin E,Peacock C,Bartholomeu DC,Haas BJ,Tran AN,Wortman JR,Alsmark UC,Angiuoli S,Anupama A,Badger J,Bringaud F,Cadag E,Carlton JM,Cerqueira GC,Creasy T,Delcher AL,Djikeng A,Embley TM,Hauser C,Ivens AC,Kummerfeld SK,Pereira-Leal JB,Nilsson D,Peterson J,Salzberg SL,Shallom J,Silva JC,Sundaram J,Westenberger S,White O,Melville SE,Donelson JE,Andersson B,Stuart KD,Hall N

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Tc00.1047053508577.40 cDNA ORF clone, Trypanosoma cruzi strain CL Brener

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