LOC662516 cDNA ORF clone, Tribolium castaneum(red flour beetle)

Gene
Clones
gRNAs

Summary

Gene Symbol	LOC662516
Entrez Gene ID	662516
Full Name	myosin 22
Synonyms	Myo22
Gene Type	protein-coding
Organism	Tribolium castaneum(red flour beetle)

mRNA and Protein(s)

mRNA	Protein	Name
XM_008197567.2	XP_008195789.1	myosin-I heavy chain

Pathways from BioSystems
Gene Ontology

The following LOC662516 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC662516 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OTg10004	XM_008197567.2 Latest version!	Tribolium castaneum myosin 22 (LOC662516), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LOC662516 ( XM_008197567.2 ) cDNA ORF clone, Tribolium castaneum(red flour beetle) -> XP_008195789.1 Tribolium castaneum myosin 22 (LOC662516), mRNA.

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CloneID	OTg10004
Clone ID Related Accession (Same CDS sequence)	XM_008197567.2
Accession Version	XM_008197567.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6444bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1458403200000
Organism	Tribolium castaneum(red flour beetle)
Product	myosin-I heavy chain
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_015450177.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Mar 21, 2016 this sequence version replaced XM_008197567.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Tribolium castaneum Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAGGAAT TAAATTTTAC TTCAACGAGC GCGATGCCCG ACGATGGCGT CCCGGACATG 
ACACGGCTGA CAATCCTCGA CGAAGAAGTG ATAAACAAGA ACCTGAAAGT GCGCTACGAA 
AAAGACCGCA TCTACACATA CACCGGCTCA ATCCTCGTCG CAGTCAACCC TTACAAGGAC 
ATTGACATCT ACTCTCAACA CTACGTGAGC AAATACCAGG GGAAAAAATT AGGGGCACAC 
GAGCCACATG TTTTCGCACT GGCGGAAGCA GCTTACGCCT CTCTTCAAGC AGATCCGAGC 
GGAAAAGGTC ACACGAACCA AAGCTTGGTC ATAAGTGGAG AAAGCGGTGC TGGAAAAACG 
GAAAACACCC GCTTTATCCT CCAATATTTA TGTTCAGTCA CTAGTGGAGT TTCGACATGG 
GTTGAGCAAC AGATCCTCGA AGCTAACACC ATATTGGAAG CTTTCGGCAA CGCGAAAACA 
GTCAGAAATG ATAACTCGAG CCGGTTCGGA AAGTTCATGC AAGTCTGTTT TGACTCACGA 
TGGATGATCG CGGGCTGTAT CATCCAGGAT TATCTCCTAG AACAATCGAG GCTGACTTTT 
CAGGGACCCG GCGAGCGCAA TTATCACGTT TTTTACCAAT TAGTCGAAGG AGCGAAACAC 
AATAAGGAGC TAGCGACACA ATTGCACCTA AAAGACGCCA GTTTTTACAA TTATCTGAAC 
CAGTCGAGTT GTGCGAAGCT TGAAGGTGAC GATAGAAGGC TGGATTCATT AAGACTGGCT 
TTTAACGTGC TTCAAGTGCC CACAAACATG TGCAATGGCA TTTTCCAAAC ACTTTCAGCC 
ATTCTCTGGC TGGGAAACCT CAGTTTCGAG GATATCGACG GCGAGCGGTG CCAACTGGCT 
GAGACAGATG CGGAAATTTT AGAAATAATC TCTTCTCTCC TAGAATTGGA GTTAGAGTCT 
TTGAAACAAG TCGTTCTTTT AAGGCAAATT AACGTTAGAG GAAATATCAC CGAAATTCCA 
CTCAAACTAC AGGAAGCAAG AGAGAATCGA CATGCGATGG CGAAAGCGCT CTATTCCCGG 
ACTTTTGCCT GGCTTATTAA CCATATCAAC AACTGCACGA ATCCGGGACA GGACTCGACG 
CGCTTTTTGG GCGTCTTGGA CATTTTTGGC TTTGAGAATT TCGCAGTCAA CTCCTTTGAA 
CAACTCTGTA TTAATTACAC CAACGAGAAG TTGCACAAGT TTTTCAACCA CTACGTATTC 
GCACTGGAGC AGGAAATCTA CGAGCAAGAG GCAATCCAGT TCAACCACAT CTTCTTCACC 
GACAACTCCC TGTGTTTGGA GCTGCTGGAG AAGCCGCCCC GTTGTGTCTT GAGGCTGCTG 
TCCGAGCAGT GCCACTTGCC GCGAGGCTGC GACGCTTCGT ACATTTCGAA TTTGCACGCG 
GAGTTTGAAA ATCACGAACG ATACGTCAAG GGGGAAGACC GGAGGCGTTG GGAAACCGAA 
TTTGGGATAA AACACTACGC CGGCTGTGTC ACTTATTGCG TTAAGGGTTT CGTCGATAAA 
AACCGAGACG TGCAACAAGA CGTTTTCTTC GATATAATTT CGCGGAGCAA AAACGAATTT 
CTCCAAGATT TATCCAAGTA TCAAGATTTA GAAAATCTCA TGGCTTCGCG TCAGATCGCG 
AACGGCGGAA CCACCGTTTC AAGGGGGACG AGCAAAGGCA AACCAACTGT TTCTGATACT 
TTCCGCCATC AGTTGCAAGC CCTTGTCGAT GTTTTGCAGT CGACGACGCC GTGGTACGTC 
CGGTGCATTA AGCCGAACAG CGAGAAACTC CCGAATGATT ATGACAGTAA TCTAGTCTTA 
GACCAATTGA AGTATTTAGG AATGTTAGAT ATTATTCGTA TACGAAAAGA AGGCTTCCCG 
ATCCACATGA ACTTTGACGA TTTCGTCAAT AGGTATCACT GTCTTTCCAA AAACCGTCTT 
CCACCTGACA TGATGAAAGC CTGTCGGGAT TTGCTCGAGA GTTACAATTT ACCAAAGACT 
GAATGGCAGT TAGGCAAAAC CAAAGTTTTC ATGCGATCTC ACATACACGA GCCTTTGGAG 
GAAGCAAGGA ACAAAATTAT TCTCTCGCGC ACTGTCATCA TCCAGAAAGT TTACCGCAGA 
TACGTCGCCC GGAAGGAGTT TTTGAAAATA CGCAACGCCG TCTTGCGCAT CCAACACGCT 
TACATCGGCT GGAAATTACG AATCGAATTT TTGCGCAAAC GACGCGCTGC GATAATAATC 
CAGAGTCACT TGCGGGGAGT TTTTGCCCGA GAGGTCGCAG CCGCGTTGCG CGAAATGAGA 
CGAGTGGAAG AAGAAATGCG AAGAAGGGAG AAGCTGGAAG AGGAACGTAA AGCCCGAGAG 
GCAGAAGAGG CCCAAAAGCG ACTGGCCGAG GAAGAGTTGA GCAAAAAGAA CGAAGATTTG 
GAAAGGAGCG AAAAGGCCGC CGAACAGGAA ATAGCCGCAT TATCGCACAT GGCCGAACAG 
TTGAAACCGC GCCTTACCGA ACCTGTGACC GAGTCGGTCG ATCTTGATAA TCTCTTTGCG 
TTCTTATCAG ACGTGCAGCC CCAAAATCGC AACCAAATCA TTGACGAAAT CGGGGAAAAG 
ATGGACGAGT TGGTTGAGGA TTTGGATGTG GAGCTGGAAA CTGTGATCCA GCAGGAATTG 
GAAGGGCTGG CTTTGGAGCA ACAACAGCCG ACTCCCCCAA AACTCGCAAT GCCTACTTTG 
CCCGAACCGA CAGGGCCTCC CCCACCTCCA CCTCCCGCGT TCCAGAACCC GCCAGAATCC 
CCACAACAGG AGGTGAAGGA GACCAATAAT GAACCCATCT ATGAGGCCGT TCTCCCACGG 
GAGGACACCA CGTGTGTGTC ACCTCCTCCA TTACCCGCGC CTCCACGGCT GCCTTCAGAA 
TCCCCCAAAG CTAGTCCGCC GGTCTCAAGA CCAAACAGCG CTATACCCCC AGCTTGGCGA 
TATAATAATC ACGAACAGGA AGCTAATAAC GCAGAAAGGG AGCAACGAAG AAAATGCCGC 
GTTGAGAAGA AGTTGCAAGA GTTGACTGAA ACTAGTGAGA AAGAAAGTTC CGCAGAAGAA 
ACCTATCATG ATATGATCGA GTTTGCTGAG AGTTATTTCA ATGCCCATGA AAGGAGTCCC 
GAGGGAACCA TTATAGCGAC TTTGACGAGG AAGAGACAGA GTTCGGAATT GATTCCGAAG 
TGTGAGATGG TGACTTATTA CAAAGGGACG ACCATTCCAA ATTCGCATAT TCATATGTAC 
GATCCCGACA ACATCAATAT TGCTTGCAAC ATCTTCAGGG ACTTGTGCAA GTACATCCGC 
GGCGAGATGA ACGCCGAGCG CGAGCTGCTC GTCATCCAGA CCATAATCGG GCACGCACTC 
GAGCGCGAGG AGATCCGCGA CGAGATCTTC GTGCAATGCA TCCGCCAAGC CACCAACAAC 
CCCAGTTCCG AAGGCACCGA ACGCGTGTGG CTTCTCCTCT GCCTCTGTGT CGTGTCCTTC 
CAGCCCTCCA AGCTCCTCCA CCGCTACTTC GTGTCATTTC TCAAGAAAAA CCTAGCACAA 
GGGGGTAAAA TCTCGCAGTA CGTGCAGTGG TGCATTGACA ACTGCAATAA CACTAAAGTG 
AAAGTGCGGG AACACCCGCC TTCGTCAGTC GAAGTAGCGG CAATGAAGCG TCTGGGAACG 
ATCGTGTGCA GATTTTTCTT TCTGGATGGG CGCACGAAGG CGATTGATGT CCACCCGACC 
GACACTGCAG GCGACGCAGC GAGGAAATTG GCTGAAAGGT TGGGGTTGCG GAATCTGGAC 
GGCTGGGCGA TTTATCAGAG TCGGCCTGAT GGGGAGGAAC ACGTCCGCGC GCATTATTAT 
CTCTACGATG TGATAGCGCA ATGGGAAATG AACCAGAATA AGTTGGCACC GCCGACCGGA 
CTCGCCACGC TCGGCCGGCG GAGTGGCACC ACGCTGAGTG GCGGAGAGAA TAGGTTTATT 
TTCAAGAGAA GGCTTTTCAA GTCTAGTCGG GAGCTGAGTC AGGATCCCGT CGAAGTCAAT 
CTTTTGTATG CTCAAGCTGT GCACAGTGTG GTCAAGAGCG ATGATTTTCC GGTTACGGAG 
AAAGTGGCCC TGCAGTTGGC GGGCCTGCAG GCCCAAGTGG CGTTGGGCAA CCCCAAAGAC 
AATAACAAGC TCGAATACTA CACCGACATT GACGTTTATT TACCATACCG GATAAGTCGT 
ACTCGAGGCG ACGACGTCTG GGTGCCTATT ATAGCGCAAG CGCACAAACA ATACGGTGCT 
AACCGTTCCG AACTAACTGC CAAGGCTCTA TACCTATCTT GCGTGATGCA GTACCCCTTA 
TACGGCACTA CTATGTTTCC GGTGACCTAT CGAGGGTACT GGTCGTACGG AAACTCCCTT 
ATTTTGGGGG TGAACAGCGA GGGGATCATG TTGATAAAAC CCGACGATAA ATTCGTCCTT 
AACGAATACC GCTATCAGGA AATCGAGAGT ATTCTCCTAG ACCCCAGTGA TAGTTTTATC 
ACCATAACGT TACAACGACA CCTCAATGAC AATAACCACA AATGTTTCGT TTTCGAGACG 
ACGCAAAAGA ACGAAATCGG GTCTCTGATT GCGAGTTATT GTCCGAGTTT AGCCGCCTGG 
CTGACGGAGA ACGAAGCTCC TCAGAAGAGG GTTAAGGGGA TTACGAACGA GGACAGAGTG 
AGGTTGTACC ACAATTTGGT TAATTGCCGA CGAGCGTTGG TCGATCATGA CATATTGAGG 
AAACCGACTG ATTCCTCAGG AAGCTTTTTA CGGAACACGT TGAGGCGGTT GAGTAAACAC 
AAGTTGGATA AGTTGAGGCA GGAACATGGA GGCGACACTG GAGAGACTTA CAAAGGGTTT 
CATTATACGT TTTGGGCGTT CAGTCGCCAG CAACTGCCAC AAAGCATCAG CAGACTGCCC 
GATCAAGAGG AGCAAATCAT GCTTCAAGTG TTTCAGATTA TTCTGACCTA CGCTGGACTA 
GGGCAGAATG GTGAGACAAT TCGGCGCGTG GAGGACGAGC ACGTGACGCT CCTGCAGACG 
ATCCTCGAGC GCTGCATGCG CAAGGAGTCG CTCCTTTGCG AGCTCTACCT CCAGCTCATA 
AAGCAAACCA CCGACCATCC CGACCCCAAC TCCCGCGTCA ACCTCCGCCA CTGGGCTTTG 
CTCTCTCTCG CATGTTCGGT GGTTTTGCCC CCCAATAAAG CGATCCGTCG CTACTTGATA 
GCCCACTTGA AGCGCTGCAG CTCCGATTAC GTGACCGAAG AAGGCAAATA CGCCCGATTT 
GCGGAAAAAT GCCTGCTCAA GACCCAGGGA ACGCGCCGCC GCCAATGGCC GCCCTCCCGT 
GAGGAGATCC TCTGCACGAT CAATCGGCGG CCGGTCTATG CCCGGTTCCA TTTCATGGAC 
GGACAGTACC ATTCCGTGGA GTTCCACCCC TCGGCAACGG CCAAGGATGT GTTGGAGATT 
GTGAGGGATA AGATTGGGTT GGGGTCGGAT GCGAAAGGAT ATGCGATTTA TGAAGTTTTG 
GGGAATTCGG AACGGAGTTT GTCGGCTGAG GAGAAAGTTG CCGATGTTAT GGCCAAGTGG 
GAGAGGTACA GAGCCGCCAC TGCGGCCAAT ACTGCCAATA GTACAGGCAC TGCCAAGAGG 
GCAAGGCCCC AACATCACTT TTTCCTATTT AAGAAGCATA TTTTTATGGA CAACTTTATC 
AACTTGAACG ATCCGGTTGA GAAGGAATTG CTGTATCATC AGGTCTTGCA TGATTTGAGG 
ACCGACAGGT TCCCGGTTAC TGACAAAGAA GCGATGATGC TAACTGCCCT CCAAGCTCAA 
CTGGAACTGG GCGACGTGAA CGACCAAATC CACGACTACC GGCCTATCGC CTGTCACTGT 
CTACCAGCCC GCCTTGTGCC GATCTTACCA CAAGAGGGCG TAGCAATGCA CCACCAATCT 
CTGCGGGGAA TGACCCCATC GGAGGCGAAG CAAGCCTTCC TAAACCTAAT TCAAAGCTGG 
CCTTTGCACA AAGCTACAAT TTTCGACGTT ATGCAATCGT TCACTTCGAA CTGGCCTCGA 
GTGTTGTGGC TCGCCGTGGA CCAGAAAGGC CTCCACCTCC TCGAGCACCG TTCAAGAAAC 
ACACTTTGTA CCTACGATTA TGGGAGTATT TTGAGCTACG CACCGGCGCT TAACTACCTA 
ATGATCATAA CCGGAAGTGA TAAGAAACAG AGTAAAGTTA TTTTGACCAC TGCCCAAGCG 
TTCCAAATCG CGACTTTGAT ACGGGAGTAC ATGGAGGTGC TGCATGGAGA TGCGGTGGAA 
ATCCGGAGGG CACAAACGCC GAAAAGTCGG CCAGTTAGTG CTTTGCACCA GAACGCACCC 
TTGGTGCCCC CCCAGCCCAG CTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008195789.1
CDS	206..6649
Translation	MQELNFTSTSAMPDDGVPDMTRLTILDEEVINKNLKVRYEKDRIYTYTGSILVAVNPYKDIDIYSQHYVSKYQGKKLGAHEPHVFALAEAAYASLQADPSGKGHTNQSLVISGESGAGKTENTRFILQYLCSVTSGVSTWVEQQILEANTILEAFGNAKTVRNDNSSRFGKFMQVCFDSRWMIAGCIIQDYLLEQSRLTFQGPGERNYHVFYQLVEGAKHNKELATQLHLKDASFYNYLNQSSCAKLEGDDRRLDSLRLAFNVLQVPTNMCNGIFQTLSAILWLGNLSFEDIDGERCQLAETDAEILEIISSLLELELESLKQVVLLRQINVRGNITEIPLKLQEARENRHAMAKALYSRTFAWLINHINNCTNPGQDSTRFLGVLDIFGFENFAVNSFEQLCINYTNEKLHKFFNHYVFALEQEIYEQEAIQFNHIFFTDNSLCLELLEKPPRCVLRLLSEQCHLPRGCDASYISNLHAEFENHERYVKGEDRRRWETEFGIKHYAGCVTYCVKGFVDKNRDVQQDVFFDIISRSKNEFLQDLSKYQDLENLMASRQIANGGTTVSRGTSKGKPTVSDTFRHQLQALVDVLQSTTPWYVRCIKPNSEKLPNDYDSNLVLDQLKYLGMLDIIRIRKEGFPIHMNFDDFVNRYHCLSKNRLPPDMMKACRDLLESYNLPKTEWQLGKTKVFMRSHIHEPLEEARNKIILSRTVIIQKVYRRYVARKEFLKIRNAVLRIQHAYIGWKLRIEFLRKRRAAIIIQSHLRGVFAREVAAALREMRRVEEEMRRREKLEEERKAREAEEAQKRLAEEELSKKNEDLERSEKAAEQEIAALSHMAEQLKPRLTEPVTESVDLDNLFAFLSDVQPQNRNQIIDEIGEKMDELVEDLDVELETVIQQELEGLALEQQQPTPPKLAMPTLPEPTGPPPPPPPAFQNPPESPQQEVKETNNEPIYEAVLPREDTTCVSPPPLPAPPRLPSESPKASPPVSRPNSAIPPAWRYNNHEQEANNAEREQRRKCRVEKKLQELTETSEKESSAEETYHDMIEFAESYFNAHERSPEGTIIATLTRKRQSSELIPKCEMVTYYKGTTIPNSHIHMYDPDNINIACNIFRDLCKYIRGEMNAERELLVIQTIIGHALEREEIRDEIFVQCIRQATNNPSSEGTERVWLLLCLCVVSFQPSKLLHRYFVSFLKKNLAQGGKISQYVQWCIDNCNNTKVKVREHPPSSVEVAAMKRLGTIVCRFFFLDGRTKAIDVHPTDTAGDAARKLAERLGLRNLDGWAIYQSRPDGEEHVRAHYYLYDVIAQWEMNQNKLAPPTGLATLGRRSGTTLSGGENRFIFKRRLFKSSRELSQDPVEVNLLYAQAVHSVVKSDDFPVTEKVALQLAGLQAQVALGNPKDNNKLEYYTDIDVYLPYRISRTRGDDVWVPIIAQAHKQYGANRSELTAKALYLSCVMQYPLYGTTMFPVTYRGYWSYGNSLILGVNSEGIMLIKPDDKFVLNEYRYQEIESILLDPSDSFITITLQRHLNDNNHKCFVFETTQKNEIGSLIASYCPSLAAWLTENEAPQKRVKGITNEDRVRLYHNLVNCRRALVDHDILRKPTDSSGSFLRNTLRRLSKHKLDKLRQEHGGDTGETYKGFHYTFWAFSRQQLPQSISRLPDQEEQIMLQVFQIILTYAGLGQNGETIRRVEDEHVTLLQTILERCMRKESLLCELYLQLIKQTTDHPDPNSRVNLRHWALLSLACSVVLPPNKAIRRYLIAHLKRCSSDYVTEEGKYARFAEKCLLKTQGTRRRQWPPSREEILCTINRRPVYARFHFMDGQYHSVEFHPSATAKDVLEIVRDKIGLGSDAKGYAIYEVLGNSERSLSAEEKVADVMAKWERYRAATAANTANSTGTAKRARPQHHFFLFKKHIFMDNFINLNDPVEKELLYHQVLHDLRTDRFPVTDKEAMMLTALQAQLELGDVNDQIHDYRPIACHCLPARLVPILPQEGVAMHHQSLRGMTPSEAKQAFLNLIQSWPLHKATIFDVMQSFTSNWPRVLWLAVDQKGLHLLEHRSRNTLCTYDYGSILSYAPALNYLMIITGSDKKQSKVILTTAQAFQIATLIREYMEVLHGDAVEIRRAQTPKSRPVSALHQNAPLVPPQPS

Target ORF information:

RefSeq Version	XM_008197567.2
Organism	Tribolium castaneum(red flour beetle)
Definition	Tribolium castaneum myosin 22 (LOC662516), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008197567.2

ORF Insert Sequence:

ATGCAGGAAT TAAATTTTAC TTCAACGAGC GCGATGCCCG ACGATGGCGT CCCGGACATG 
ACACGGCTGA CAATCCTCGA CGAAGAAGTG ATAAACAAGA ACCTGAAAGT GCGCTACGAA 
AAAGACCGCA TCTACACATA CACCGGCTCA ATCCTCGTCG CAGTCAACCC TTACAAGGAC 
ATTGACATCT ACTCTCAACA CTACGTGAGC AAATACCAGG GGAAAAAATT AGGGGCACAC 
GAGCCACATG TTTTCGCACT GGCGGAAGCA GCTTACGCCT CTCTTCAAGC AGATCCGAGC 
GGAAAAGGTC ACACGAACCA AAGCTTGGTC ATAAGTGGAG AAAGCGGTGC TGGAAAAACG 
GAAAACACCC GCTTTATCCT CCAATATTTA TGTTCAGTCA CTAGTGGAGT TTCGACATGG 
GTTGAGCAAC AGATCCTCGA AGCTAACACC ATATTGGAAG CTTTCGGCAA CGCGAAAACA 
GTCAGAAATG ATAACTCGAG CCGGTTCGGA AAGTTCATGC AAGTCTGTTT TGACTCACGA 
TGGATGATCG CGGGCTGTAT CATCCAGGAT TATCTCCTAG AACAATCGAG GCTGACTTTT 
CAGGGACCCG GCGAGCGCAA TTATCACGTT TTTTACCAAT TAGTCGAAGG AGCGAAACAC 
AATAAGGAGC TAGCGACACA ATTGCACCTA AAAGACGCCA GTTTTTACAA TTATCTGAAC 
CAGTCGAGTT GTGCGAAGCT TGAAGGTGAC GATAGAAGGC TGGATTCATT AAGACTGGCT 
TTTAACGTGC TTCAAGTGCC CACAAACATG TGCAATGGCA TTTTCCAAAC ACTTTCAGCC 
ATTCTCTGGC TGGGAAACCT CAGTTTCGAG GATATCGACG GCGAGCGGTG CCAACTGGCT 
GAGACAGATG CGGAAATTTT AGAAATAATC TCTTCTCTCC TAGAATTGGA GTTAGAGTCT 
TTGAAACAAG TCGTTCTTTT AAGGCAAATT AACGTTAGAG GAAATATCAC CGAAATTCCA 
CTCAAACTAC AGGAAGCAAG AGAGAATCGA CATGCGATGG CGAAAGCGCT CTATTCCCGG 
ACTTTTGCCT GGCTTATTAA CCATATCAAC AACTGCACGA ATCCGGGACA GGACTCGACG 
CGCTTTTTGG GCGTCTTGGA CATTTTTGGC TTTGAGAATT TCGCAGTCAA CTCCTTTGAA 
CAACTCTGTA TTAATTACAC CAACGAGAAG TTGCACAAGT TTTTCAACCA CTACGTATTC 
GCACTGGAGC AGGAAATCTA CGAGCAAGAG GCAATCCAGT TCAACCACAT CTTCTTCACC 
GACAACTCCC TGTGTTTGGA GCTGCTGGAG AAGCCGCCCC GTTGTGTCTT GAGGCTGCTG 
TCCGAGCAGT GCCACTTGCC GCGAGGCTGC GACGCTTCGT ACATTTCGAA TTTGCACGCG 
GAGTTTGAAA ATCACGAACG ATACGTCAAG GGGGAAGACC GGAGGCGTTG GGAAACCGAA 
TTTGGGATAA AACACTACGC CGGCTGTGTC ACTTATTGCG TTAAGGGTTT CGTCGATAAA 
AACCGAGACG TGCAACAAGA CGTTTTCTTC GATATAATTT CGCGGAGCAA AAACGAATTT 
CTCCAAGATT TATCCAAGTA TCAAGATTTA GAAAATCTCA TGGCTTCGCG TCAGATCGCG 
AACGGCGGAA CCACCGTTTC AAGGGGGACG AGCAAAGGCA AACCAACTGT TTCTGATACT 
TTCCGCCATC AGTTGCAAGC CCTTGTCGAT GTTTTGCAGT CGACGACGCC GTGGTACGTC 
CGGTGCATTA AGCCGAACAG CGAGAAACTC CCGAATGATT ATGACAGTAA TCTAGTCTTA 
GACCAATTGA AGTATTTAGG AATGTTAGAT ATTATTCGTA TACGAAAAGA AGGCTTCCCG 
ATCCACATGA ACTTTGACGA TTTCGTCAAT AGGTATCACT GTCTTTCCAA AAACCGTCTT 
CCACCTGACA TGATGAAAGC CTGTCGGGAT TTGCTCGAGA GTTACAATTT ACCAAAGACT 
GAATGGCAGT TAGGCAAAAC CAAAGTTTTC ATGCGATCTC ACATACACGA GCCTTTGGAG 
GAAGCAAGGA ACAAAATTAT TCTCTCGCGC ACTGTCATCA TCCAGAAAGT TTACCGCAGA 
TACGTCGCCC GGAAGGAGTT TTTGAAAATA CGCAACGCCG TCTTGCGCAT CCAACACGCT 
TACATCGGCT GGAAATTACG AATCGAATTT TTGCGCAAAC GACGCGCTGC GATAATAATC 
CAGAGTCACT TGCGGGGAGT TTTTGCCCGA GAGGTCGCAG CCGCGTTGCG CGAAATGAGA 
CGAGTGGAAG AAGAAATGCG AAGAAGGGAG AAGCTGGAAG AGGAACGTAA AGCCCGAGAG 
GCAGAAGAGG CCCAAAAGCG ACTGGCCGAG GAAGAGTTGA GCAAAAAGAA CGAAGATTTG 
GAAAGGAGCG AAAAGGCCGC CGAACAGGAA ATAGCCGCAT TATCGCACAT GGCCGAACAG 
TTGAAACCGC GCCTTACCGA ACCTGTGACC GAGTCGGTCG ATCTTGATAA TCTCTTTGCG 
TTCTTATCAG ACGTGCAGCC CCAAAATCGC AACCAAATCA TTGACGAAAT CGGGGAAAAG 
ATGGACGAGT TGGTTGAGGA TTTGGATGTG GAGCTGGAAA CTGTGATCCA GCAGGAATTG 
GAAGGGCTGG CTTTGGAGCA ACAACAGCCG ACTCCCCCAA AACTCGCAAT GCCTACTTTG 
CCCGAACCGA CAGGGCCTCC CCCACCTCCA CCTCCCGCGT TCCAGAACCC GCCAGAATCC 
CCACAACAGG AGGTGAAGGA GACCAATAAT GAACCCATCT ATGAGGCCGT TCTCCCACGG 
GAGGACACCA CGTGTGTGTC ACCTCCTCCA TTACCCGCGC CTCCACGGCT GCCTTCAGAA 
TCCCCCAAAG CTAGTCCGCC GGTCTCAAGA CCAAACAGCG CTATACCCCC AGCTTGGCGA 
TATAATAATC ACGAACAGGA AGCTAATAAC GCAGAAAGGG AGCAACGAAG AAAATGCCGC 
GTTGAGAAGA AGTTGCAAGA GTTGACTGAA ACTAGTGAGA AAGAAAGTTC CGCAGAAGAA 
ACCTATCATG ATATGATCGA GTTTGCTGAG AGTTATTTCA ATGCCCATGA AAGGAGTCCC 
GAGGGAACCA TTATAGCGAC TTTGACGAGG AAGAGACAGA GTTCGGAATT GATTCCGAAG 
TGTGAGATGG TGACTTATTA CAAAGGGACG ACCATTCCAA ATTCGCATAT TCATATGTAC 
GATCCCGACA ACATCAATAT TGCTTGCAAC ATCTTCAGGG ACTTGTGCAA GTACATCCGC 
GGCGAGATGA ACGCCGAGCG CGAGCTGCTC GTCATCCAGA CCATAATCGG GCACGCACTC 
GAGCGCGAGG AGATCCGCGA CGAGATCTTC GTGCAATGCA TCCGCCAAGC CACCAACAAC 
CCCAGTTCCG AAGGCACCGA ACGCGTGTGG CTTCTCCTCT GCCTCTGTGT CGTGTCCTTC 
CAGCCCTCCA AGCTCCTCCA CCGCTACTTC GTGTCATTTC TCAAGAAAAA CCTAGCACAA 
GGGGGTAAAA TCTCGCAGTA CGTGCAGTGG TGCATTGACA ACTGCAATAA CACTAAAGTG 
AAAGTGCGGG AACACCCGCC TTCGTCAGTC GAAGTAGCGG CAATGAAGCG TCTGGGAACG 
ATCGTGTGCA GATTTTTCTT TCTGGATGGG CGCACGAAGG CGATTGATGT CCACCCGACC 
GACACTGCAG GCGACGCAGC GAGGAAATTG GCTGAAAGGT TGGGGTTGCG GAATCTGGAC 
GGCTGGGCGA TTTATCAGAG TCGGCCTGAT GGGGAGGAAC ACGTCCGCGC GCATTATTAT 
CTCTACGATG TGATAGCGCA ATGGGAAATG AACCAGAATA AGTTGGCACC GCCGACCGGA 
CTCGCCACGC TCGGCCGGCG GAGTGGCACC ACGCTGAGTG GCGGAGAGAA TAGGTTTATT 
TTCAAGAGAA GGCTTTTCAA GTCTAGTCGG GAGCTGAGTC AGGATCCCGT CGAAGTCAAT 
CTTTTGTATG CTCAAGCTGT GCACAGTGTG GTCAAGAGCG ATGATTTTCC GGTTACGGAG 
AAAGTGGCCC TGCAGTTGGC GGGCCTGCAG GCCCAAGTGG CGTTGGGCAA CCCCAAAGAC 
AATAACAAGC TCGAATACTA CACCGACATT GACGTTTATT TACCATACCG GATAAGTCGT 
ACTCGAGGCG ACGACGTCTG GGTGCCTATT ATAGCGCAAG CGCACAAACA ATACGGTGCT 
AACCGTTCCG AACTAACTGC CAAGGCTCTA TACCTATCTT GCGTGATGCA GTACCCCTTA 
TACGGCACTA CTATGTTTCC GGTGACCTAT CGAGGGTACT GGTCGTACGG AAACTCCCTT 
ATTTTGGGGG TGAACAGCGA GGGGATCATG TTGATAAAAC CCGACGATAA ATTCGTCCTT 
AACGAATACC GCTATCAGGA AATCGAGAGT ATTCTCCTAG ACCCCAGTGA TAGTTTTATC 
ACCATAACGT TACAACGACA CCTCAATGAC AATAACCACA AATGTTTCGT TTTCGAGACG 
ACGCAAAAGA ACGAAATCGG GTCTCTGATT GCGAGTTATT GTCCGAGTTT AGCCGCCTGG 
CTGACGGAGA ACGAAGCTCC TCAGAAGAGG GTTAAGGGGA TTACGAACGA GGACAGAGTG 
AGGTTGTACC ACAATTTGGT TAATTGCCGA CGAGCGTTGG TCGATCATGA CATATTGAGG 
AAACCGACTG ATTCCTCAGG AAGCTTTTTA CGGAACACGT TGAGGCGGTT GAGTAAACAC 
AAGTTGGATA AGTTGAGGCA GGAACATGGA GGCGACACTG GAGAGACTTA CAAAGGGTTT 
CATTATACGT TTTGGGCGTT CAGTCGCCAG CAACTGCCAC AAAGCATCAG CAGACTGCCC 
GATCAAGAGG AGCAAATCAT GCTTCAAGTG TTTCAGATTA TTCTGACCTA CGCTGGACTA 
GGGCAGAATG GTGAGACAAT TCGGCGCGTG GAGGACGAGC ACGTGACGCT CCTGCAGACG 
ATCCTCGAGC GCTGCATGCG CAAGGAGTCG CTCCTTTGCG AGCTCTACCT CCAGCTCATA 
AAGCAAACCA CCGACCATCC CGACCCCAAC TCCCGCGTCA ACCTCCGCCA CTGGGCTTTG 
CTCTCTCTCG CATGTTCGGT GGTTTTGCCC CCCAATAAAG CGATCCGTCG CTACTTGATA 
GCCCACTTGA AGCGCTGCAG CTCCGATTAC GTGACCGAAG AAGGCAAATA CGCCCGATTT 
GCGGAAAAAT GCCTGCTCAA GACCCAGGGA ACGCGCCGCC GCCAATGGCC GCCCTCCCGT 
GAGGAGATCC TCTGCACGAT CAATCGGCGG CCGGTCTATG CCCGGTTCCA TTTCATGGAC 
GGACAGTACC ATTCCGTGGA GTTCCACCCC TCGGCAACGG CCAAGGATGT GTTGGAGATT 
GTGAGGGATA AGATTGGGTT GGGGTCGGAT GCGAAAGGAT ATGCGATTTA TGAAGTTTTG 
GGGAATTCGG AACGGAGTTT GTCGGCTGAG GAGAAAGTTG CCGATGTTAT GGCCAAGTGG 
GAGAGGTACA GAGCCGCCAC TGCGGCCAAT ACTGCCAATA GTACAGGCAC TGCCAAGAGG 
GCAAGGCCCC AACATCACTT TTTCCTATTT AAGAAGCATA TTTTTATGGA CAACTTTATC 
AACTTGAACG ATCCGGTTGA GAAGGAATTG CTGTATCATC AGGTCTTGCA TGATTTGAGG 
ACCGACAGGT TCCCGGTTAC TGACAAAGAA GCGATGATGC TAACTGCCCT CCAAGCTCAA 
CTGGAACTGG GCGACGTGAA CGACCAAATC CACGACTACC GGCCTATCGC CTGTCACTGT 
CTACCAGCCC GCCTTGTGCC GATCTTACCA CAAGAGGGCG TAGCAATGCA CCACCAATCT 
CTGCGGGGAA TGACCCCATC GGAGGCGAAG CAAGCCTTCC TAAACCTAAT TCAAAGCTGG 
CCTTTGCACA AAGCTACAAT TTTCGACGTT ATGCAATCGT TCACTTCGAA CTGGCCTCGA 
GTGTTGTGGC TCGCCGTGGA CCAGAAAGGC CTCCACCTCC TCGAGCACCG TTCAAGAAAC 
ACACTTTGTA CCTACGATTA TGGGAGTATT TTGAGCTACG CACCGGCGCT TAACTACCTA 
ATGATCATAA CCGGAAGTGA TAAGAAACAG AGTAAAGTTA TTTTGACCAC TGCCCAAGCG 
TTCCAAATCG CGACTTTGAT ACGGGAGTAC ATGGAGGTGC TGCATGGAGA TGCGGTGGAA 
ATCCGGAGGG CACAAACGCC GAAAAGTCGG CCAGTTAGTG CTTTGCACCA GAACGCACCC 
TTGGTGCCCC CCCAGCCCAG CTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

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LOC662516 cDNA ORF clone, Tribolium castaneum(red flour beetle)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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