TGME49_277550 cDNA ORF clone, Toxoplasma gondii ME49

Gene
Clones
gRNAs

Summary

Gene Symbol	TGME49_277550
Entrez Gene ID	7898758
Full Name	UvrD/REP helicase domain-containing protein
Synonyms	TGME49_077550
Gene Type	protein-coding
Organism	Toxoplasma gondii ME49

mRNA and Protein(s)

mRNA	Protein	Name
XM_018781771.1	XP_018635165.1	UvrD/REP helicase domain-containing protein

Pathways from BioSystems
Gene Ontology

The following TGME49_277550 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the TGME49_277550 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OTi12247	XM_018781771.1 Latest version!	Toxoplasma gondii ME49 UvrD/REP helicase domain-containing protein partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

TGME49_277550 ( XM_018781771.1 ) cDNA ORF clone, Toxoplasma gondii ME49 -> XP_018635165.1 Toxoplasma gondii ME49 UvrD/REP helicase domain-containing protein partial mRNA.

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CloneID	OTi12247
Clone ID Related Accession (Same CDS sequence)	XM_018781771.1
Accession Version	XM_018781771.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 9573bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1476806400000
Organism	Toxoplasma gondii ME49
Product	UvrD/REP helicase domain-containing protein
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NC_031480). COMPLETENESS: incomplete on both ends.

ATGGAGAATA CGTCTCTCAG TAGAGTACCC GAACGGGGAA GCCGCAGTCG AGATTCGCCT 
CGTCCTCCCG GCTCTTCCTC CCTCCACGCC TCATCTCAGC CTCAGTCGCC GCTTCTCGCG 
CGCGACATCC GACCTGCGGG GGCCCTCTCG GGTGTATGGA CAGTCCGGAG CCTCGACGCT 
GGCATCCCGC CTCACGCCGG ATCGGTGACG AAACGTTTTG CGTCTTCTGC ATCTTCTCTG 
TCTCTTCCAG AGACTCAGCG TCATGAGCAA GCGAGAAATT CTCAGGCCTG CCTCCTGGAC 
AGGTCCTCTC CCAGGAGCCT GGCGAGCTCG CAGCTGCGAC TGGAGCCCGC GAAGCCGTGG 
GTGAGTTTCC AGAGAAGCAG AGCACAGAGA ACCGATCGAG CTCACGTGGA CGCGACGCTC 
ATGAGTGAGG CTGGGGACCA GAACTCCCGA ACTCCCGGTG TCTCCGCGAA CGCCTCCTCG 
GTTTCGGTGG AGCAGATGCG ACTTCTGCCG CGAGAAGGCC AGAAGTCGTC TGACGCGCGC 
GACGCCGGCT CAGCCAACCA AGAATGTCCG CGGACATCGC AGACGCCGTC GCTCGAAAGG 
CTTCCTGCGC CGCTTCCCAC AGGGCTCGCT TCGACGACCC TCTTGCGAGA AGGCAGTGCT 
GCTGTGTTTG CTTCCAAGGG AATTCCAGGA AGAAAGACGT CGGAAAACAA GTCGTCTCTC 
TCTCCACCGG GCGCCGTCTC ACACTCTGTG ACCCAGCAAC GCCCCGCTCC TCACAGGTCG 
CGTCCGCTGT GGACCTCGGG TGTCTGGACA CCTCACCATG AAGCCGACCG AGAAGAAGGA 
AGAGAAGGAC TCGGCTCTTG TGCTTCTTCG AATGCGGAGA GGAATGCGGT GCGCTCGGGT 
ACGAGGGAGA CAGAGTTGAA GGGGAGGAAG GAGGAGGAGA GCCGAGCGTT GTCAAGGACA 
GAGATGAAAA CGAATCCGAG TCCAGCTAGG ACTAGCGGGT CGCCTCAGAA CAGGCCCCGA 
CAGCCCCGAC CCCCCGTTTC GTCTGGTTTC CATCCTGCGT CTGCTCCCTT CCATTCTCGG 
CCACTTGGTG CCTCACCCTC GTCCATATCT CCGTCATCAT CTTCTTCTTT ATCCTCTTAT 
TCTTGTGCTT CGTCATCATC ATCATCTTCT TTATCCTCTT ATTCTTGTGC TTCGTCTTCC 
TCGTCTTCTC CTTCGTCTTC GTCGCCTCTT TCCACTGCTT CACGTCCCTC CGCGACTTCC 
GGTCCTGCCG TCTCTCTCGT CGGTGCCACT GGCCTACGCG TTCCCGCGAA CTCCACTGTT 
CCAGCGTGCG GCGCCTCTGC GTCTCTCGCG TCTCATCCAG TCTCCTCTGA GGAGGAGCGC 
AGAAGAAGCG TAGCGATGGT TCGCTCTCTC TCCTCTGCTG CTGTTTCCTC GTTTCAGTCT 
TCTTTCGTTC CGTCTGCATG CGTCCAATCC TCTCCGACAC CTGCCTGTGC TTCTTCGCGA 
CCGCCTGCCT CGTCTTTCTC CTCCTTGTCT GCCTCCTTGG CGTCTTCGGC TGCGTCGTCG 
ACTAGGCTCG CTTCAGACCC TTCGCAGTCT TCGCTTTCTT CACAATCTTT GCATTCTTCA 
CACTCTTTGC ATTCTTCGCT TCCTTCGTCT CTTCCATGGA CGTCTCCCGG AAGGAGTCGC 
GGGCTTTCGA GTGCGGTCCC TCTCAGCCCC TCTGCTCCCC GTTTACATTC GGAGTCTTCT 
GACGGTACGG AGAAGCCGCC GGCCGCCTTT GGCCTGCCTG CCGGCGATTC GCTTCCCTCG 
GCTTCTCGCG AGCTGCTGCG GCGCTCTATA CCCTTCAATG CGTCTTCGCT CGGCGGTGGA 
AAGACTCAGC CTCCCTCGAG TCGGTCTGCG ACCTTGCCTT CGAGTGCACC GTCGTCCTTC 
GCTTCCGCCA CGACCCGCGA TCCCGGCGTC TCTCCCAGTC GACACACATC TGATTTCTCT 
CGCCCCGTTG TTGTTCGTTG CTCATCCCCC TCTTCGTCGC CTTCGCCTTC GCGCGCTTCG 
ACTCCTGCAC CATCGTCACC TGTTTTGCCT TCAGCTGCTT CTTCTGCTTC GTCGTACGTT 
TTGGTTTCTT CTCTGCGTCC TCCCAGGAGA CCCTCTGCAT TCCTCAAGCC TTCTGTCATC 
TCTTCCGCGT CTCTCCCGTC ACTGCCTGGC TCTGCTGGGG GGGCGTCTTC GGAGAGTTCA 
GCCATATTTC AGAGTGACAC TGACGCGATG GAAACCACTC GTGGAGGGAA TGGACAGCAA 
TGTGAAGATA AGGACAGGAA TCAGAACGGC CTCTACGTAG ACGAGAGAGC GCGGCGGAGG 
CGTACACCCG TTGTGCGAGC GCATGAGGCG AAGAAGGAAG GAGGAAAGAG AATGAGAAGC 
GACGGCGAGG AGCGAGAGAG AGAAGCTGAG GGCCTCGCGT TTCGTGCTGT GCTTCCCGAG 
AAGGCGAAGG GGACGCGACG GGAGGAGACT GAGGAGGAAG TCTGTTTGAA AGAAGAAGCG 
AAAGAAGAGG GAGATGCAGA GAAAGCCGTC AGAATCTGTG AAGCGGAGAA GTTGCTCCCG 
CCTCACAGCA AGATTTTCGG ACAACTCTCG GAGGAACAAC GAAGAGTCGT CCTTGCCCCT 
GCGCATGCAA ATCTCTGCGT CATTGCTGGG CCTGGATCAG GGAAAACGAC GGCGATAACC 
GCTCGAATTC TTCGCCTCCT TCTTGAAGGC GAAGGTCCAA TCTTGGCTCT CACCTTCACG 
CGGCGAGGAG CCGAGGAGCT CCGGACGCGC GTGGTGGACG GTTTCTCTTC CACTCTCGCG 
GAGATGCGGC TTCTGTCGGG GTCGCGAGAG AACGGCGCCC TCCCCTCAGC TGTCTCTCCG 
CGAACGAACG CAGCCACAAG CTCCACAGCC TCCGCGAGAA CAAACGGCGG CGACGCCACG 
ATCTTCAACG CCCCGCTCGC GAAGCTCGAC TGGCAGCGGC AGACGAACTG GACGCATCCC 
TTCGCTCCAG CGTCTGCGGC AGGCCCTCAC CCGAGGTGCG ACTCCGCGCC TGTCCACCCG 
TCGGTCACCT GTTACTCTTC TTCGGCAGTC GCGCCTTCTG CTTCCGCCTC TTTCGCGTCG 
CTCTCTGCGT CTTTGGGAGA GCAAGCTCCA ACGTCCGCAG CTGCGGCGCC CGGCTTGCCC 
CTCTTTCACG ACCTCGCCGG TCGCGTCCTT GTCGGAACGT TTCACAAATT CGGTCTCCTC 
GTCCTCCGGC GCCACGGGCG CGCCGTGGGG GTCCCTCCCT CCTTCCTCGT CGCCACCGCG 
ACTCGCCAGG GCCAGTTGGC CCGCGAGGTC CTCGAGGCCT TCAAGGCCCG CGAGGAGGCC 
CGCGGGGCGA TGCTGAAGAG CGCGCGCGAG GAGCCAGGGA GGCCGCAGCG CGCGTGTGGG 
GTGGGGAATT TGGAGGCCGC CTTTCAGCAG GTTCGGCGCT TGCACTTGCT CCGCATGCAG 
AGAGAGCTGG CGGAGAGACA GAGAGAGAGA GCCGCCCGCT CCCGGGGCGG CGGCCCGGAG 
ACAGCAGAGT CACGAGCGAC AGGAAAGACG GGAGAGACAG GAGAGGCAGA TATCTGTCTA 
GACGAGGCGG CTCTCGACAG TGACCCAGAT GACTTGCTTG ACTTTCTGAC AGACGAAGAG 
GAGGAAGAAA GCGGGGGCTT CCACTCGCAG CACTCGGGAC TTCCGGCGGC GGGCAGAACA 
ACCAGGCGAG AGCCCGGCGA GGCCTGCGGC GGTCGGCAGC CTTCGCGGGG GCCGGTGGCG 
CCTCATATGG GGAGTCGTGT GGGGTCGACG AAATCGCGAA CGACGTGGAA AGAAGTGGAC 
ATGTTCCTGA GGCTCGTACG AAAGTGCAAG TTCTCCGAAG CGTTCCTCGA CACCCTCAAA 
ACAGAGAACG CCCAGCTGTA CGCGCTGACA CGGCAGTACG CGGCGCTACT GCGACAGCAG 
ACGCCTCCCC TCCTCGACTG CTCAGACCTC ATTCTCCTCA CTCTCGCACT GCTAGAGAGT 
CAACCAAACA TCCGGAGAGA CCTGGCAACA GCGTACCCCA TCGTCGTCGT CGACGAGTTC 
CAAGACACCA GTCTCCCCCA GTTCAAAGTT GTCAAGGCCC TGGCTCTCGG CCGCCAAGAA 
CAAGAGAGAG AACAAGCGAG AGAACGAGCA TGGATGCATG AGACTATGGA GAGAGAACGT 
GGAGAAGGAC GCGCGGGCTT CTGCGCCGGA AATCCAGGGA GGCAAGAAGC AACGAGACAA 
AGGCGAGGCG GGGTCACAGT GGTTGGAGAT GACGACCAAT CCATTTACGG ATTCCGAGGC 
ATCGACGCCA AAAATTTTTT GATTTTCGAT CGCGCTTTCC CCGATCGTTT GGAATTTCAC 
CTCTCCTGCA ACTATCGCTC CGTTGCCCCC ATCGTCTCTG CGAGTTTGGC GCTGATCAAC 
CACAATCGCC ACCGGAGACT CAAACGCCTC TACCCCGCCG CTCGGCCCTT TTCGTCTTTT 
CTCTCTTCTT TCCTGTTTCA AGCCGTTCTG CCTCCACATC AGATGCCCTC TCCCCCTTTG 
GGCTCTGCTT CAGCCGCCAT TCACTACACT CCTTCCCATC AAGCTAACGC CCTCACAAAC 
AGTTCTGCCG CTGCTTCCTC CTGTCCTCCG TCTGCTCCTG GATGTTCTTC TGCTGCCGCA 
TCATCAACAC CGTCTTCGTC TTCTTCTTCT TGTTCTTCTC TCGCGTCGTC TCCGTCGCCG 
TCGGCAGGTC TTCTGTCGCT GTTTCCACCT GCAATGTGTG CGGTGCTGTC TTCTCCGAAG 
GCGGAGGCTG CCTACATTCT TCGGTTCATC GTCGCGCTCA AGCAGACGCG AAAGTTGTGT 
TGGGGAGACT TTGTGATTCT CTCCCGCACG AACAGAGGCC TGAAGGAGTT GGAAGAGCTG 
CTGCAAGATC CCCAGGTTCT TCACTCCGCA TTTCGTTCGT CGCCTTTGCC CGACGCGGGA 
CCGTTGCCCA TCCTCTCTCT CTCCCCTTCA CTCGGGGAAG ATGAAGTGGA AAACCAAAGA 
GCGTCTTCTT CGGCTTCTTC TCCGTCGTCT CTCGCGGCTC CTCGTTCTCC TCTTGCGTCT 
TTGTCTTCGA CTCTGTGTTT GTCTCCAGTG ACATCTCCGC CGTCTCTGTG GTATCGACCA 
GACCGACCGC CAGATCCCTC GCTGTTGTGT CCTCTCTGGG CGTCGTCTGC GTTTCCAGAG 
GCACTGCCGC TGCACAGCAC CGCTCTAAAG CGTCGCGGGA CTCAGATGCT GCGGAAACAA 
GGCGTACTTC TCGTCTGTGG CTACTTGCGA CTCGCTCTCG ATCCTCACCA CGACGCGTCT 
TTCCTGCGGG TGCTCAACCG ACCCAGACGC GGCCTCGGTC TCGCCGTCGT CAGGGCGCTG 
TGCCGATGTC TGCAGACGCT CGAGGGGGAC GCTGCGTCTC CCGCCGAAGC CGGCAAGCCT 
CGTAGCGAAG CAGGAAACGA AGAGACGGAC GCGGCAGAAG AAGTCGAAGA AGGAGAGAGG 
GAAGGAGTTG AGAGAGAAGG AGAGGGGAAG GGAGGACGCA GGAGAAAGCG AGGGCGAGAG 
GCGCGAGCCC AGGAGGGAGC AAGGCAGGAC GGGGAGGAAG GCGAAGAAGG AGAGGGAGGC 
GGAAGAAGAA AAGAGGCGGT AACGAAGAGT CTGGTGTGCT GGGATGCGAA GTGGAGAGAA 
GGCCCTCTCG AGGGAATCGA CCGTCTCCGG AAACACGAAC GGCAGTGTGA CGCCCATCGT 
GGGTCGCGCG TTTTCTCCCT CTACGAGGCG GCCTCCATCA TCGCTGAACT CGAAGAAACA 
GCAGAGAACG GCGAAGGAGA GCGAAAACGC AACGACGAAA CAAACACACA GCAGGAAGGA 
GAAGCAGGCA CGTCTTCTCG ATCTTCATCT TCTTCATCGG CAAATCCGTC GTTTTCTACA 
CCTTCACCTT CGGTCTCCTC GGTCTCTGGT TCATCGCCTT CTTCGGTGGA CTTGCCTGTT 
GAAGACCATG CGACCGAACG GCTGTCGAGG GAGAAGAAGG AAACTCGATT GCTGTCTGGG 
TCTTTACTGA AGCAGACAAA ATGCCCTGCC TCTGCGCTGC CGCGACTGCG GCGTTTCCTC 
GAGCAGCTCC AGGCGCTGAG AAGCTTGGCG AGGAGTCGTA CTGCGGGAGC CTCCGACCTC 
GTCCTCTTCG TCCTCAAAGC CTTTGGCCTC GAGGCCTTCC TCGCAGGTGT CTCCGGGGAG 
ACAGAAGGCA CAGAGACGCT CCCGAAGACG GAGACAGAGA AACAAACGAC GGAGACGGCC 
GATCAGAAGA CCCTGGAGAG GAGGAAGGAG AGGGCGGAGA AGAAGAAGGG AAGGAAGAAG 
GCGGCTGGCG CTGGACGAGA CGATGGGGAT GAGGTCTGCC GGCAAGCGGA AGAGGAAACA 
TGGGGAGAGT GTGGAGCAGG CGAGAAGAGA GGCGACAGCG CCGTAAGAGC TTCCGGAGAG 
GGGACAGAGC GAGAGATGCA GGCGAAGAAG TTGAGGAAGA AGAACGACGG CCCCGATCGA 
GAAAAAAGGA CCCCCATCGA GGAGAAGCGA CAGACAGAAA AGAGAGACAG AGAATCGAAG 
ACGGATCCGA CGTCTTCGAC ATCTGTGTGT TCTTCTGAAA ATAAACTGGA AGACGCCTGG 
ACGCTCCTGA GGCTTGCTGA GGGATACACG CCGAATGCGT TGCAGCCGAC AGGGTGGGAT 
TGTCTCTCGC GTTTTCTGAA AGATTTGACG AACGACAGAT GCGGAGAAGC AGAGACGCTT 
CAGCGAAGAA ACTCGATTTT CCTCTCCACC ATTCATCAAG CCAAAGGCCT CGAATGGCCG 
GTCGTCATCC TCGCGAAAGC CAACGAGGGA CATCTTCCAC TGTCCAACGA AGACACCTCG 
GGATCGTTTG TGAGCCTTCC AGCGCGGATC CCATTGACAT CTGTTTCCTC GTTCTCCTCT 
TCGGTTCCGC CCTCCTCGTC CCCGCCGTTC TCTCCCTCTC GTTCCTCCTC GGTTTTGCCT 
ACCAACCGAG CAACCTCGTT CACTTCGTCC TTTACTCCAT CTTCCTCCTT TACGACTCCA 
TCTTCCTCCT TTACTACTCC ATCTTCGTCT TCTTCCTTTG GTTCATCTTC TTCCTCTTCC 
TGCGGTGTGC CGTCTTCTTT CTCCTCTTGC TGTAGCGCGT CTTCGTCCGC TTCGTCTTCT 
TCTTCGTTCG TCTTGGTTCC TCCTCTGTCT TCTTCGTCGT CCGCCGCTGC CTCCGCTGTC 
TCCTCTGTCT CCGCTGTCTC TGCCTCGCTG ACCGCGGAGG CCTGGCAGAA GCGTCGCACG 
CAAGAAGAGC GAGTGTGGGC GATGTGGACC GAGAAGAAGA AGCTCGAGGA GCAGGAGCTC 
GAGGAGGAAC GGCGTCTCTG CTACGTCGCG CTGACGAGGG CAAAGAGCAT GCTCCTCGTC 
ACTTGCTCGA AGACGGAGAA GAGCGGCCAG AGTCTCCGAG TTTCGCGCTT TGTTTCCGAG 
GCGAAACTCT TCCACTTCCA CCCGCTCTCG GCGGCCTCGG GCGTCCATCC CAAGTCTGAG 
AAAGACCCAT GTCGGTGCCG CAGCGCAGGA ACCTCGACCG ACGCCCGAGG CTCGAGAGAA 
GAAGAGACGG AGACCGACAG GGACGCGCAT GCAGAGCGAC GAAAACGTGA GGAGAGGAAC 
AGCCAACTCG GACACCGAGT CGCGAAAGGA AGATTACAGG AGGAAGGAGA GACTGGCGGC 
AACGAAGAGG AATCCCTAAC GAAAGGCAAG CTCACAGCGG AGGGCCAAAA AAAAGAAGAA 
GACTGGACAG GAGAGACGAA GGCACACCAG AACGGGACCG ACACAGAGAG TGAGCGCAAC 
AGAGAGATGA AGATGAAGGG AGAACATGAC GCGAGCGAAG AAGATATCAA AATCGACACA 
GACATCAGTG ACCTTCTGGA TTTGAGCAGC GTTTCGGAGA GAACAGGACA GTGGGGGCGA 
CTCCTGGAAG CAAGAAAAAA ACAGCAACAG AACCGTTCCT CTTCGTTCAG GTGTACGTCC 
CCTTCGTTCG GGCGTCTCGC GGCACCTTCA GCTGCTTCTC ACTCGGTTTC CGTCTCGAAT 
GAGGGAGTGC GTCCTGTCTC CGACGAGTCC TCCTCTTCCC CCTCTGAACT CGCGAAGGCG 
CTGCCTGCTG GAGCGCTTGG CGAAGGGAGA CGGCCTTCGC GATGTGGAGA CCTGAGCAGA 
GGGGAGACGC TGGACGCAAG TTCAGAGGGG GAAGAGCTGA AGAAACATGC AGGCGAGCGC 
GAGAGCGAAG AACGAAGTCG AACGCCTTCG CCGACAGAAG GCCCTGCAAG AAGAGAGGTT 
ATAGAAGAGG CTAGTGAGCG TCGAGGAGGC GACCCGAGAG CAGCAGAAGA GAAGCGGAGA 
GAAGAAACAA AAGCCGAGCA AGACGAAGAG AAACTCCTTC CATCGACATG CGCGCCGAAG 
AGAGAAGAAC AATACGAACG TCGGAACGCA GAGGATACGC AGAGGGAAGA AGCATCTCAG 
AGAGACGCAC AAGAAGCAGA GGCAGAAGAA GAACTGCAGA GGAAAGAAGA ATCCCGGAAC 
TCCAATCGAC AGACGCTGGA GATGTACGCG TTCGAGCCAG AAGACGACGA AGTCATCAGT 
TTGGGAAGTG ATCTTGACGA GCCGACAGCA GGGCCGATGG ACCCCTGCAG GGACGCGCCA 
ACGCCGGGGA AGGAGCCGGA GGGAAAGAGA CGCTCTGAGA TCGCCTGTTC GTCGCATGCA 
GAAATCCGAG AGGTTGTGAC AGCTCTCGAG GAAGCTGAAG AAGAGACTGG CGAAGAAGGG 
GAAGAGACAG GGAACGCGAT AGCGGAGAAA GAGAACTACG AGCAACCGAC AGCCGAAGAG 
AAGGACGAAC GAGTCGACGT CAGAGAGCAG AGCAAGGACT CGAGAGAAAA GGAGACAAGG 
CAGACTCCAA GGGTGGCAAG AAAGGCGACA GAGAGGAGAG ATGGGGAAGC AGATGTGATT 
GTCATCGACG AAGAAGAAGC GCCGGAAACG GCTCACGACA GAGAAACAGA GAAAACGCAA 
GAGACGACCG CAGAGCCAGA CAGCGTGTCG AAGGGCGAAG ACGAGATGGA GGAACCGACA 
GCGGTGGAAG TGGAAGACGA TGACGAAGTC GAAGCTCAAA GAGACCAAGA CGAAAGTGCG 
AGAAGCACAG ACGAAAACGA AGAAGGCGGA AGGAAAACAA GTAGCAAAGC CTTCTTCCGC 
AGCGCTCAAG ACCTGGCAAG ACGCGTCGCG CAGGCCTCCC GATCCTCGTC AGACTTTGCT 
AGACCAACAG CCTCCCTCCA TCGAGACGGC CACCATACAG CACTCAAACA CCGCGAGTTA 
CAGACTTCCA CGTTTTCTCT TCCTTCTTCT TCGCCTTCTT CTTCTTCGCC TTCTTCTTCT 
TCGCCTTCTT CTTCTCCTCT TCCATCTGCT TCGTCTTCGT CTTCAGGTTC TGCTCTCTCC 
TCTTCTGGGA GGTTGGAGGC TCCCGCGCGT TCGCTGGGCA GCGCCGCGGC ACCGGGCCGT 
CCGTGGCGGT GTTTCCTTTT GAAGAAAAAG TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_018635165.1
CDS	1..9573
Translation	MENTSLSRVPERGSRSRDSPRPPGSSSLHASSQPQSPLLARDIRPAGALSGVWTVRSLDAGIPPHAGSVTKRFASSASSLSLPETQRHEQARNSQACLLDRSSPRSLASSQLRLEPAKPWVSFQRSRAQRTDRAHVDATLMSEAGDQNSRTPGVSANASSVSVEQMRLLPREGQKSSDARDAGSANQECPRTSQTPSLERLPAPLPTGLASTTLLREGSAAVFASKGIPGRKTSENKSSLSPPGAVSHSVTQQRPAPHRSRPLWTSGVWTPHHEADREEGREGLGSCASSNAERNAVRSGTRETELKGRKEEESRALSRTEMKTNPSPARTSGSPQNRPRQPRPPVSSGFHPASAPFHSRPLGASPSSISPSSSSSLSSYSCASSSSSSSLSSYSCASSSSSSPSSSSPLSTASRPSATSGPAVSLVGATGLRVPANSTVPACGASASLASHPVSSEEERRRSVAMVRSLSSAAVSSFQSSFVPSACVQSSPTPACASSRPPASSFSSLSASLASSAASSTRLASDPSQSSLSSQSLHSSHSLHSSLPSSLPWTSPGRSRGLSSAVPLSPSAPRLHSESSDGTEKPPAAFGLPAGDSLPSASRELLRRSIPFNASSLGGGKTQPPSSRSATLPSSAPSSFASATTRDPGVSPSRHTSDFSRPVVVRCSSPSSSPSPSRASTPAPSSPVLPSAASSASSYVLVSSLRPPRRPSAFLKPSVISSASLPSLPGSAGGASSESSAIFQSDTDAMETTRGGNGQQCEDKDRNQNGLYVDERARRRRTPVVRAHEAKKEGGKRMRSDGEEREREAEGLAFRAVLPEKAKGTRREETEEEVCLKEEAKEEGDAEKAVRICEAEKLLPPHSKIFGQLSEEQRRVVLAPAHANLCVIAGPGSGKTTAITARILRLLLEGEGPILALTFTRRGAEELRTRVVDGFSSTLAEMRLLSGSRENGALPSAVSPRTNAATSSTASARTNGGDATIFNAPLAKLDWQRQTNWTHPFAPASAAGPHPRCDSAPVHPSVTCYSSSAVAPSASASFASLSASLGEQAPTSAAAAPGLPLFHDLAGRVLVGTFHKFGLLVLRRHGRAVGVPPSFLVATATRQGQLAREVLEAFKAREEARGAMLKSAREEPGRPQRACGVGNLEAAFQQVRRLHLLRMQRELAERQRERAARSRGGGPETAESRATGKTGETGEADICLDEAALDSDPDDLLDFLTDEEEEESGGFHSQHSGLPAAGRTTRREPGEACGGRQPSRGPVAPHMGSRVGSTKSRTTWKEVDMFLRLVRKCKFSEAFLDTLKTENAQLYALTRQYAALLRQQTPPLLDCSDLILLTLALLESQPNIRRDLATAYPIVVVDEFQDTSLPQFKVVKALALGRQEQEREQARERAWMHETMERERGEGRAGFCAGNPGRQEATRQRRGGVTVVGDDDQSIYGFRGIDAKNFLIFDRAFPDRLEFHLSCNYRSVAPIVSASLALINHNRHRRLKRLYPAARPFSSFLSSFLFQAVLPPHQMPSPPLGSASAAIHYTPSHQANALTNSSAAASSCPPSAPGCSSAAASSTPSSSSSSCSSLASSPSPSAGLLSLFPPAMCAVLSSPKAEAAYILRFIVALKQTRKLCWGDFVILSRTNRGLKELEELLQDPQVLHSAFRSSPLPDAGPLPILSLSPSLGEDEVENQRASSSASSPSSLAAPRSPLASLSSTLCLSPVTSPPSLWYRPDRPPDPSLLCPLWASSAFPEALPLHSTALKRRGTQMLRKQGVLLVCGYLRLALDPHHDASFLRVLNRPRRGLGLAVVRALCRCLQTLEGDAASPAEAGKPRSEAGNEETDAAEEVEEGEREGVEREGEGKGGRRRKRGREARAQEGARQDGEEGEEGEGGGRRKEAVTKSLVCWDAKWREGPLEGIDRLRKHERQCDAHRGSRVFSLYEAASIIAELEETAENGEGERKRNDETNTQQEGEAGTSSRSSSSSSANPSFSTPSPSVSSVSGSSPSSVDLPVEDHATERLSREKKETRLLSGSLLKQTKCPASALPRLRRFLEQLQALRSLARSRTAGASDLVLFVLKAFGLEAFLAGVSGETEGTETLPKTETEKQTTETADQKTLERRKERAEKKKGRKKAAGAGRDDGDEVCRQAEEETWGECGAGEKRGDSAVRASGEGTEREMQAKKLRKKNDGPDREKRTPIEEKRQTEKRDRESKTDPTSSTSVCSSENKLEDAWTLLRLAEGYTPNALQPTGWDCLSRFLKDLTNDRCGEAETLQRRNSIFLSTIHQAKGLEWPVVILAKANEGHLPLSNEDTSGSFVSLPARIPLTSVSSFSSSVPPSSSPPFSPSRSSSVLPTNRATSFTSSFTPSSSFTTPSSSFTTPSSSSSFGSSSSSSCGVPSSFSSCCSASSSASSSSSFVLVPPLSSSSSAAASAVSSVSAVSASLTAEAWQKRRTQEERVWAMWTEKKKLEEQELEEERRLCYVALTRAKSMLLVTCSKTEKSGQSLRVSRFVSEAKLFHFHPLSAASGVHPKSEKDPCRCRSAGTSTDARGSREEETETDRDAHAERRKREERNSQLGHRVAKGRLQEEGETGGNEEESLTKGKLTAEGQKKEEDWTGETKAHQNGTDTESERNREMKMKGEHDASEEDIKIDTDISDLLDLSSVSERTGQWGRLLEARKKQQQNRSSSFRCTSPSFGRLAAPSAASHSVSVSNEGVRPVSDESSSSPSELAKALPAGALGEGRRPSRCGDLSRGETLDASSEGEELKKHAGERESEERSRTPSPTEGPARREVIEEASERRGGDPRAAEEKRREETKAEQDEEKLLPSTCAPKREEQYERRNAEDTQREEASQRDAQEAEAEEELQRKEESRNSNRQTLEMYAFEPEDDEVISLGSDLDEPTAGPMDPCRDAPTPGKEPEGKRRSEIACSSHAEIREVVTALEEAEEETGEEGEETGNAIAEKENYEQPTAEEKDERVDVREQSKDSREKETRQTPRVARKATERRDGEADVIVIDEEEAPETAHDRETEKTQETTAEPDSVSKGEDEMEEPTAVEVEDDDEVEAQRDQDESARSTDENEEGGRKTSSKAFFRSAQDLARRVAQASRSSSDFARPTASLHRDGHHTALKHRELQTSTFSLPSSSPSSSSPSSSSPSSSPLPSASSSSSGSALSSSGRLEAPARSLGSAAAPGRPWRCFLLKKK

Target ORF information:

RefSeq Version	XM_018781771.1
Organism	Toxoplasma gondii ME49
Definition	Toxoplasma gondii ME49 UvrD/REP helicase domain-containing protein partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_018781771.1

ORF Insert Sequence:

ATGGAGAATA CGTCTCTCAG TAGAGTACCC GAACGGGGAA GCCGCAGTCG AGATTCGCCT 
CGTCCTCCCG GCTCTTCCTC CCTCCACGCC TCATCTCAGC CTCAGTCGCC GCTTCTCGCG 
CGCGACATCC GACCTGCGGG GGCCCTCTCG GGTGTATGGA CAGTCCGGAG CCTCGACGCT 
GGCATCCCGC CTCACGCCGG ATCGGTGACG AAACGTTTTG CGTCTTCTGC ATCTTCTCTG 
TCTCTTCCAG AGACTCAGCG TCATGAGCAA GCGAGAAATT CTCAGGCCTG CCTCCTGGAC 
AGGTCCTCTC CCAGGAGCCT GGCGAGCTCG CAGCTGCGAC TGGAGCCCGC GAAGCCGTGG 
GTGAGTTTCC AGAGAAGCAG AGCACAGAGA ACCGATCGAG CTCACGTGGA CGCGACGCTC 
ATGAGTGAGG CTGGGGACCA GAACTCCCGA ACTCCCGGTG TCTCCGCGAA CGCCTCCTCG 
GTTTCGGTGG AGCAGATGCG ACTTCTGCCG CGAGAAGGCC AGAAGTCGTC TGACGCGCGC 
GACGCCGGCT CAGCCAACCA AGAATGTCCG CGGACATCGC AGACGCCGTC GCTCGAAAGG 
CTTCCTGCGC CGCTTCCCAC AGGGCTCGCT TCGACGACCC TCTTGCGAGA AGGCAGTGCT 
GCTGTGTTTG CTTCCAAGGG AATTCCAGGA AGAAAGACGT CGGAAAACAA GTCGTCTCTC 
TCTCCACCGG GCGCCGTCTC ACACTCTGTG ACCCAGCAAC GCCCCGCTCC TCACAGGTCG 
CGTCCGCTGT GGACCTCGGG TGTCTGGACA CCTCACCATG AAGCCGACCG AGAAGAAGGA 
AGAGAAGGAC TCGGCTCTTG TGCTTCTTCG AATGCGGAGA GGAATGCGGT GCGCTCGGGT 
ACGAGGGAGA CAGAGTTGAA GGGGAGGAAG GAGGAGGAGA GCCGAGCGTT GTCAAGGACA 
GAGATGAAAA CGAATCCGAG TCCAGCTAGG ACTAGCGGGT CGCCTCAGAA CAGGCCCCGA 
CAGCCCCGAC CCCCCGTTTC GTCTGGTTTC CATCCTGCGT CTGCTCCCTT CCATTCTCGG 
CCACTTGGTG CCTCACCCTC GTCCATATCT CCGTCATCAT CTTCTTCTTT ATCCTCTTAT 
TCTTGTGCTT CGTCATCATC ATCATCTTCT TTATCCTCTT ATTCTTGTGC TTCGTCTTCC 
TCGTCTTCTC CTTCGTCTTC GTCGCCTCTT TCCACTGCTT CACGTCCCTC CGCGACTTCC 
GGTCCTGCCG TCTCTCTCGT CGGTGCCACT GGCCTACGCG TTCCCGCGAA CTCCACTGTT 
CCAGCGTGCG GCGCCTCTGC GTCTCTCGCG TCTCATCCAG TCTCCTCTGA GGAGGAGCGC 
AGAAGAAGCG TAGCGATGGT TCGCTCTCTC TCCTCTGCTG CTGTTTCCTC GTTTCAGTCT 
TCTTTCGTTC CGTCTGCATG CGTCCAATCC TCTCCGACAC CTGCCTGTGC TTCTTCGCGA 
CCGCCTGCCT CGTCTTTCTC CTCCTTGTCT GCCTCCTTGG CGTCTTCGGC TGCGTCGTCG 
ACTAGGCTCG CTTCAGACCC TTCGCAGTCT TCGCTTTCTT CACAATCTTT GCATTCTTCA 
CACTCTTTGC ATTCTTCGCT TCCTTCGTCT CTTCCATGGA CGTCTCCCGG AAGGAGTCGC 
GGGCTTTCGA GTGCGGTCCC TCTCAGCCCC TCTGCTCCCC GTTTACATTC GGAGTCTTCT 
GACGGTACGG AGAAGCCGCC GGCCGCCTTT GGCCTGCCTG CCGGCGATTC GCTTCCCTCG 
GCTTCTCGCG AGCTGCTGCG GCGCTCTATA CCCTTCAATG CGTCTTCGCT CGGCGGTGGA 
AAGACTCAGC CTCCCTCGAG TCGGTCTGCG ACCTTGCCTT CGAGTGCACC GTCGTCCTTC 
GCTTCCGCCA CGACCCGCGA TCCCGGCGTC TCTCCCAGTC GACACACATC TGATTTCTCT 
CGCCCCGTTG TTGTTCGTTG CTCATCCCCC TCTTCGTCGC CTTCGCCTTC GCGCGCTTCG 
ACTCCTGCAC CATCGTCACC TGTTTTGCCT TCAGCTGCTT CTTCTGCTTC GTCGTACGTT 
TTGGTTTCTT CTCTGCGTCC TCCCAGGAGA CCCTCTGCAT TCCTCAAGCC TTCTGTCATC 
TCTTCCGCGT CTCTCCCGTC ACTGCCTGGC TCTGCTGGGG GGGCGTCTTC GGAGAGTTCA 
GCCATATTTC AGAGTGACAC TGACGCGATG GAAACCACTC GTGGAGGGAA TGGACAGCAA 
TGTGAAGATA AGGACAGGAA TCAGAACGGC CTCTACGTAG ACGAGAGAGC GCGGCGGAGG 
CGTACACCCG TTGTGCGAGC GCATGAGGCG AAGAAGGAAG GAGGAAAGAG AATGAGAAGC 
GACGGCGAGG AGCGAGAGAG AGAAGCTGAG GGCCTCGCGT TTCGTGCTGT GCTTCCCGAG 
AAGGCGAAGG GGACGCGACG GGAGGAGACT GAGGAGGAAG TCTGTTTGAA AGAAGAAGCG 
AAAGAAGAGG GAGATGCAGA GAAAGCCGTC AGAATCTGTG AAGCGGAGAA GTTGCTCCCG 
CCTCACAGCA AGATTTTCGG ACAACTCTCG GAGGAACAAC GAAGAGTCGT CCTTGCCCCT 
GCGCATGCAA ATCTCTGCGT CATTGCTGGG CCTGGATCAG GGAAAACGAC GGCGATAACC 
GCTCGAATTC TTCGCCTCCT TCTTGAAGGC GAAGGTCCAA TCTTGGCTCT CACCTTCACG 
CGGCGAGGAG CCGAGGAGCT CCGGACGCGC GTGGTGGACG GTTTCTCTTC CACTCTCGCG 
GAGATGCGGC TTCTGTCGGG GTCGCGAGAG AACGGCGCCC TCCCCTCAGC TGTCTCTCCG 
CGAACGAACG CAGCCACAAG CTCCACAGCC TCCGCGAGAA CAAACGGCGG CGACGCCACG 
ATCTTCAACG CCCCGCTCGC GAAGCTCGAC TGGCAGCGGC AGACGAACTG GACGCATCCC 
TTCGCTCCAG CGTCTGCGGC AGGCCCTCAC CCGAGGTGCG ACTCCGCGCC TGTCCACCCG 
TCGGTCACCT GTTACTCTTC TTCGGCAGTC GCGCCTTCTG CTTCCGCCTC TTTCGCGTCG 
CTCTCTGCGT CTTTGGGAGA GCAAGCTCCA ACGTCCGCAG CTGCGGCGCC CGGCTTGCCC 
CTCTTTCACG ACCTCGCCGG TCGCGTCCTT GTCGGAACGT TTCACAAATT CGGTCTCCTC 
GTCCTCCGGC GCCACGGGCG CGCCGTGGGG GTCCCTCCCT CCTTCCTCGT CGCCACCGCG 
ACTCGCCAGG GCCAGTTGGC CCGCGAGGTC CTCGAGGCCT TCAAGGCCCG CGAGGAGGCC 
CGCGGGGCGA TGCTGAAGAG CGCGCGCGAG GAGCCAGGGA GGCCGCAGCG CGCGTGTGGG 
GTGGGGAATT TGGAGGCCGC CTTTCAGCAG GTTCGGCGCT TGCACTTGCT CCGCATGCAG 
AGAGAGCTGG CGGAGAGACA GAGAGAGAGA GCCGCCCGCT CCCGGGGCGG CGGCCCGGAG 
ACAGCAGAGT CACGAGCGAC AGGAAAGACG GGAGAGACAG GAGAGGCAGA TATCTGTCTA 
GACGAGGCGG CTCTCGACAG TGACCCAGAT GACTTGCTTG ACTTTCTGAC AGACGAAGAG 
GAGGAAGAAA GCGGGGGCTT CCACTCGCAG CACTCGGGAC TTCCGGCGGC GGGCAGAACA 
ACCAGGCGAG AGCCCGGCGA GGCCTGCGGC GGTCGGCAGC CTTCGCGGGG GCCGGTGGCG 
CCTCATATGG GGAGTCGTGT GGGGTCGACG AAATCGCGAA CGACGTGGAA AGAAGTGGAC 
ATGTTCCTGA GGCTCGTACG AAAGTGCAAG TTCTCCGAAG CGTTCCTCGA CACCCTCAAA 
ACAGAGAACG CCCAGCTGTA CGCGCTGACA CGGCAGTACG CGGCGCTACT GCGACAGCAG 
ACGCCTCCCC TCCTCGACTG CTCAGACCTC ATTCTCCTCA CTCTCGCACT GCTAGAGAGT 
CAACCAAACA TCCGGAGAGA CCTGGCAACA GCGTACCCCA TCGTCGTCGT CGACGAGTTC 
CAAGACACCA GTCTCCCCCA GTTCAAAGTT GTCAAGGCCC TGGCTCTCGG CCGCCAAGAA 
CAAGAGAGAG AACAAGCGAG AGAACGAGCA TGGATGCATG AGACTATGGA GAGAGAACGT 
GGAGAAGGAC GCGCGGGCTT CTGCGCCGGA AATCCAGGGA GGCAAGAAGC AACGAGACAA 
AGGCGAGGCG GGGTCACAGT GGTTGGAGAT GACGACCAAT CCATTTACGG ATTCCGAGGC 
ATCGACGCCA AAAATTTTTT GATTTTCGAT CGCGCTTTCC CCGATCGTTT GGAATTTCAC 
CTCTCCTGCA ACTATCGCTC CGTTGCCCCC ATCGTCTCTG CGAGTTTGGC GCTGATCAAC 
CACAATCGCC ACCGGAGACT CAAACGCCTC TACCCCGCCG CTCGGCCCTT TTCGTCTTTT 
CTCTCTTCTT TCCTGTTTCA AGCCGTTCTG CCTCCACATC AGATGCCCTC TCCCCCTTTG 
GGCTCTGCTT CAGCCGCCAT TCACTACACT CCTTCCCATC AAGCTAACGC CCTCACAAAC 
AGTTCTGCCG CTGCTTCCTC CTGTCCTCCG TCTGCTCCTG GATGTTCTTC TGCTGCCGCA 
TCATCAACAC CGTCTTCGTC TTCTTCTTCT TGTTCTTCTC TCGCGTCGTC TCCGTCGCCG 
TCGGCAGGTC TTCTGTCGCT GTTTCCACCT GCAATGTGTG CGGTGCTGTC TTCTCCGAAG 
GCGGAGGCTG CCTACATTCT TCGGTTCATC GTCGCGCTCA AGCAGACGCG AAAGTTGTGT 
TGGGGAGACT TTGTGATTCT CTCCCGCACG AACAGAGGCC TGAAGGAGTT GGAAGAGCTG 
CTGCAAGATC CCCAGGTTCT TCACTCCGCA TTTCGTTCGT CGCCTTTGCC CGACGCGGGA 
CCGTTGCCCA TCCTCTCTCT CTCCCCTTCA CTCGGGGAAG ATGAAGTGGA AAACCAAAGA 
GCGTCTTCTT CGGCTTCTTC TCCGTCGTCT CTCGCGGCTC CTCGTTCTCC TCTTGCGTCT 
TTGTCTTCGA CTCTGTGTTT GTCTCCAGTG ACATCTCCGC CGTCTCTGTG GTATCGACCA 
GACCGACCGC CAGATCCCTC GCTGTTGTGT CCTCTCTGGG CGTCGTCTGC GTTTCCAGAG 
GCACTGCCGC TGCACAGCAC CGCTCTAAAG CGTCGCGGGA CTCAGATGCT GCGGAAACAA 
GGCGTACTTC TCGTCTGTGG CTACTTGCGA CTCGCTCTCG ATCCTCACCA CGACGCGTCT 
TTCCTGCGGG TGCTCAACCG ACCCAGACGC GGCCTCGGTC TCGCCGTCGT CAGGGCGCTG 
TGCCGATGTC TGCAGACGCT CGAGGGGGAC GCTGCGTCTC CCGCCGAAGC CGGCAAGCCT 
CGTAGCGAAG CAGGAAACGA AGAGACGGAC GCGGCAGAAG AAGTCGAAGA AGGAGAGAGG 
GAAGGAGTTG AGAGAGAAGG AGAGGGGAAG GGAGGACGCA GGAGAAAGCG AGGGCGAGAG 
GCGCGAGCCC AGGAGGGAGC AAGGCAGGAC GGGGAGGAAG GCGAAGAAGG AGAGGGAGGC 
GGAAGAAGAA AAGAGGCGGT AACGAAGAGT CTGGTGTGCT GGGATGCGAA GTGGAGAGAA 
GGCCCTCTCG AGGGAATCGA CCGTCTCCGG AAACACGAAC GGCAGTGTGA CGCCCATCGT 
GGGTCGCGCG TTTTCTCCCT CTACGAGGCG GCCTCCATCA TCGCTGAACT CGAAGAAACA 
GCAGAGAACG GCGAAGGAGA GCGAAAACGC AACGACGAAA CAAACACACA GCAGGAAGGA 
GAAGCAGGCA CGTCTTCTCG ATCTTCATCT TCTTCATCGG CAAATCCGTC GTTTTCTACA 
CCTTCACCTT CGGTCTCCTC GGTCTCTGGT TCATCGCCTT CTTCGGTGGA CTTGCCTGTT 
GAAGACCATG CGACCGAACG GCTGTCGAGG GAGAAGAAGG AAACTCGATT GCTGTCTGGG 
TCTTTACTGA AGCAGACAAA ATGCCCTGCC TCTGCGCTGC CGCGACTGCG GCGTTTCCTC 
GAGCAGCTCC AGGCGCTGAG AAGCTTGGCG AGGAGTCGTA CTGCGGGAGC CTCCGACCTC 
GTCCTCTTCG TCCTCAAAGC CTTTGGCCTC GAGGCCTTCC TCGCAGGTGT CTCCGGGGAG 
ACAGAAGGCA CAGAGACGCT CCCGAAGACG GAGACAGAGA AACAAACGAC GGAGACGGCC 
GATCAGAAGA CCCTGGAGAG GAGGAAGGAG AGGGCGGAGA AGAAGAAGGG AAGGAAGAAG 
GCGGCTGGCG CTGGACGAGA CGATGGGGAT GAGGTCTGCC GGCAAGCGGA AGAGGAAACA 
TGGGGAGAGT GTGGAGCAGG CGAGAAGAGA GGCGACAGCG CCGTAAGAGC TTCCGGAGAG 
GGGACAGAGC GAGAGATGCA GGCGAAGAAG TTGAGGAAGA AGAACGACGG CCCCGATCGA 
GAAAAAAGGA CCCCCATCGA GGAGAAGCGA CAGACAGAAA AGAGAGACAG AGAATCGAAG 
ACGGATCCGA CGTCTTCGAC ATCTGTGTGT TCTTCTGAAA ATAAACTGGA AGACGCCTGG 
ACGCTCCTGA GGCTTGCTGA GGGATACACG CCGAATGCGT TGCAGCCGAC AGGGTGGGAT 
TGTCTCTCGC GTTTTCTGAA AGATTTGACG AACGACAGAT GCGGAGAAGC AGAGACGCTT 
CAGCGAAGAA ACTCGATTTT CCTCTCCACC ATTCATCAAG CCAAAGGCCT CGAATGGCCG 
GTCGTCATCC TCGCGAAAGC CAACGAGGGA CATCTTCCAC TGTCCAACGA AGACACCTCG 
GGATCGTTTG TGAGCCTTCC AGCGCGGATC CCATTGACAT CTGTTTCCTC GTTCTCCTCT 
TCGGTTCCGC CCTCCTCGTC CCCGCCGTTC TCTCCCTCTC GTTCCTCCTC GGTTTTGCCT 
ACCAACCGAG CAACCTCGTT CACTTCGTCC TTTACTCCAT CTTCCTCCTT TACGACTCCA 
TCTTCCTCCT TTACTACTCC ATCTTCGTCT TCTTCCTTTG GTTCATCTTC TTCCTCTTCC 
TGCGGTGTGC CGTCTTCTTT CTCCTCTTGC TGTAGCGCGT CTTCGTCCGC TTCGTCTTCT 
TCTTCGTTCG TCTTGGTTCC TCCTCTGTCT TCTTCGTCGT CCGCCGCTGC CTCCGCTGTC 
TCCTCTGTCT CCGCTGTCTC TGCCTCGCTG ACCGCGGAGG CCTGGCAGAA GCGTCGCACG 
CAAGAAGAGC GAGTGTGGGC GATGTGGACC GAGAAGAAGA AGCTCGAGGA GCAGGAGCTC 
GAGGAGGAAC GGCGTCTCTG CTACGTCGCG CTGACGAGGG CAAAGAGCAT GCTCCTCGTC 
ACTTGCTCGA AGACGGAGAA GAGCGGCCAG AGTCTCCGAG TTTCGCGCTT TGTTTCCGAG 
GCGAAACTCT TCCACTTCCA CCCGCTCTCG GCGGCCTCGG GCGTCCATCC CAAGTCTGAG 
AAAGACCCAT GTCGGTGCCG CAGCGCAGGA ACCTCGACCG ACGCCCGAGG CTCGAGAGAA 
GAAGAGACGG AGACCGACAG GGACGCGCAT GCAGAGCGAC GAAAACGTGA GGAGAGGAAC 
AGCCAACTCG GACACCGAGT CGCGAAAGGA AGATTACAGG AGGAAGGAGA GACTGGCGGC 
AACGAAGAGG AATCCCTAAC GAAAGGCAAG CTCACAGCGG AGGGCCAAAA AAAAGAAGAA 
GACTGGACAG GAGAGACGAA GGCACACCAG AACGGGACCG ACACAGAGAG TGAGCGCAAC 
AGAGAGATGA AGATGAAGGG AGAACATGAC GCGAGCGAAG AAGATATCAA AATCGACACA 
GACATCAGTG ACCTTCTGGA TTTGAGCAGC GTTTCGGAGA GAACAGGACA GTGGGGGCGA 
CTCCTGGAAG CAAGAAAAAA ACAGCAACAG AACCGTTCCT CTTCGTTCAG GTGTACGTCC 
CCTTCGTTCG GGCGTCTCGC GGCACCTTCA GCTGCTTCTC ACTCGGTTTC CGTCTCGAAT 
GAGGGAGTGC GTCCTGTCTC CGACGAGTCC TCCTCTTCCC CCTCTGAACT CGCGAAGGCG 
CTGCCTGCTG GAGCGCTTGG CGAAGGGAGA CGGCCTTCGC GATGTGGAGA CCTGAGCAGA 
GGGGAGACGC TGGACGCAAG TTCAGAGGGG GAAGAGCTGA AGAAACATGC AGGCGAGCGC 
GAGAGCGAAG AACGAAGTCG AACGCCTTCG CCGACAGAAG GCCCTGCAAG AAGAGAGGTT 
ATAGAAGAGG CTAGTGAGCG TCGAGGAGGC GACCCGAGAG CAGCAGAAGA GAAGCGGAGA 
GAAGAAACAA AAGCCGAGCA AGACGAAGAG AAACTCCTTC CATCGACATG CGCGCCGAAG 
AGAGAAGAAC AATACGAACG TCGGAACGCA GAGGATACGC AGAGGGAAGA AGCATCTCAG 
AGAGACGCAC AAGAAGCAGA GGCAGAAGAA GAACTGCAGA GGAAAGAAGA ATCCCGGAAC 
TCCAATCGAC AGACGCTGGA GATGTACGCG TTCGAGCCAG AAGACGACGA AGTCATCAGT 
TTGGGAAGTG ATCTTGACGA GCCGACAGCA GGGCCGATGG ACCCCTGCAG GGACGCGCCA 
ACGCCGGGGA AGGAGCCGGA GGGAAAGAGA CGCTCTGAGA TCGCCTGTTC GTCGCATGCA 
GAAATCCGAG AGGTTGTGAC AGCTCTCGAG GAAGCTGAAG AAGAGACTGG CGAAGAAGGG 
GAAGAGACAG GGAACGCGAT AGCGGAGAAA GAGAACTACG AGCAACCGAC AGCCGAAGAG 
AAGGACGAAC GAGTCGACGT CAGAGAGCAG AGCAAGGACT CGAGAGAAAA GGAGACAAGG 
CAGACTCCAA GGGTGGCAAG AAAGGCGACA GAGAGGAGAG ATGGGGAAGC AGATGTGATT 
GTCATCGACG AAGAAGAAGC GCCGGAAACG GCTCACGACA GAGAAACAGA GAAAACGCAA 
GAGACGACCG CAGAGCCAGA CAGCGTGTCG AAGGGCGAAG ACGAGATGGA GGAACCGACA 
GCGGTGGAAG TGGAAGACGA TGACGAAGTC GAAGCTCAAA GAGACCAAGA CGAAAGTGCG 
AGAAGCACAG ACGAAAACGA AGAAGGCGGA AGGAAAACAA GTAGCAAAGC CTTCTTCCGC 
AGCGCTCAAG ACCTGGCAAG ACGCGTCGCG CAGGCCTCCC GATCCTCGTC AGACTTTGCT 
AGACCAACAG CCTCCCTCCA TCGAGACGGC CACCATACAG CACTCAAACA CCGCGAGTTA 
CAGACTTCCA CGTTTTCTCT TCCTTCTTCT TCGCCTTCTT CTTCTTCGCC TTCTTCTTCT 
TCGCCTTCTT CTTCTCCTCT TCCATCTGCT TCGTCTTCGT CTTCAGGTTC TGCTCTCTCC 
TCTTCTGGGA GGTTGGAGGC TCCCGCGCGT TCGCTGGGCA GCGCCGCGGC ACCGGGCCGT 
CCGTGGCGGT GTTTCCTTTT GAAGAAAAAG TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

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TGME49_277550 cDNA ORF clone, Toxoplasma gondii ME49

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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