TTHERM_00727710 cDNA ORF clone, Tetrahymena thermophila SB210

Gene
Clones
gRNAs

Summary

Gene Symbol	TTHERM_00727710
Entrez Gene ID	7846297
Full Name	transmembrane protein, putative
Gene Type	protein-coding
Organism	Tetrahymena thermophila SB210

mRNA and Protein(s)

mRNA	Protein	Name
XM_001022656.2	XP_001022656.2	transmembrane protein, putative

Pathways from BioSystems
Gene Ontology

The following TTHERM_00727710 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the TTHERM_00727710 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OTe55147	XM_001022656.2 Latest version!	Tetrahymena thermophila SB210 transmembrane protein, putative partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1301.30 $1859.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

TTHERM_00727710 ( XM_001022656.2 ) cDNA ORF clone, Tetrahymena thermophila SB210 -> XP_001022656.2 Tetrahymena thermophila SB210 transmembrane protein, putative partial mRNA.

Price & Availability↑

CloneID	OTe55147
Clone ID Related Accession (Same CDS sequence)	XM_001022656.2
Accession Version	XM_001022656.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7602bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1433692800000
Organism	Tetrahymena thermophila SB210
Product	transmembrane protein, putative
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NW_002476251). On Jun 9, 2015 this sequence version replaced XM_001022656.1. COMPLETENESS: incomplete on both ends.

ATGAATTCTA ACTCAAATTA AAAACCTGAT GGTCAAATAC TAAAAAAGTA AGCAACTCTA 
CAAAGATTAA ATTCTCGAAT AAATGAATAA TTCATTAAAA GCAAGCTCCT TTAAATGAAA 
AACGATTATA AACAAAAGAA ATTTCATAAT TAGATACCCT CAAGATCAAT TTTAATATGC 
CAAGACGAAT AAGATAATCT ACGCTCATTT TTTGTATAAA ATTCAGCTAA TATAGATAAT 
AAAATTGAAG AAAAAGAAAA ATATGTAGGG GAAATTTAGA ATTTTATTAG TGTAGATGAT 
AAAAATAATG AAGAATACGA AGAAGATATT TCTGACGTTG AAAGCAACAA AGATGATAAC 
TAAGATGATT TTATTTAAGA AAGCCAATTA ATATCTGATA AAGCTGAAAA GGAATACTTA 
GAAGAATGTT AGCTTGGTTA AATTAAATTA AAGCAAATAG AATCTGTAGC GTTGAATGAA 
AAAATATATG ATAATAACAA AGAAAAAGAA ACTGATTAAC TAACAAATAA CGAAAAATCT 
TTATAATTGA GAAATAAGTT AAGTGGTAGT AAGACTAATA TTATATAAAA ATAGTAACAT 
AATGATGAGC AAAATATTCA ATTACCTCAG TAAAATAATT TATCTTAAAC AAAATAATAA 
TAATCAGGTC AAATGAAAAA CTCTACAATC AATTAAAAAA AATTAATTGA ATTTAATATA 
AAAATTATCA TGAAAAATTA AAAATAGGAT AAAAATTTAA TCTTAGAATA TGGTGATTTC 
CTTGATTTGA TTGAAGAAGT AACATTCGAG AATATAAGGA CAATAAATGT ATTCAAGATA 
TTAAAAGTAT TTTAAAGAAT AAAGATTTTG AGAGTTGTTT ATTCAATATT CGATATCAAT 
AGCTGCAGAT AGTATATATT TGATTGTAAA AATTATTTAG AAAATATATT CTTTTTGAGA 
TCTAGTCCAG CTGTATTTTC TAGTAGAATT TTCAAAAAAG CAGATTAGAT AGAAAATATA 
ACCAGAGAAT CAGCGATGTT TATAATGTTT TAACAAGATT ATTTAAAAAT CTAATATATT 
GAATAGATTG ATTAAATAGT CTTAGATTTG TTAGAGTTAA ATACTTTTTA ATCAGTAAGA 
ATTATCTAAA ATTACAATCC TCTCATATTA GTAGATTATT AGTAATTACT TGGTGAATAT 
AGATAACTTA TTTTATAAAA TACTAATGAG GAAGACCAAA AGTCTAAAAA TACTTTATAA 
GATTTTGAAG AGGATTAATA TTTATCCAGA TTAGAAGAAG GAACAGCTGA TTTTTATAAT 
TACCTGTTTC TAGATATTAA AAACGAAGAA TCTTTAAATG ACGAAGAAAA AAATTAACTT 
AAATAAATAA AATTTTATAA AAGTGTCTCT AGTATAGAGT AAGACATTTT TAGGAAAAAG 
AAATTCGATA GCTTTGTAAT AGAGAAGCGT TTTTCGAGTA AGAAGAATCC AAAAAAAGTA 
AAAACAAGAA TAGAACTTAT AATAAATAAA TTTCATTTTG AAGACATACC ATCCTTTATT 
TACTTGACTA CTATAATAGA CTAGTATTCT AGTATAATTA CGTCTATTAA GTTTATGAGA 
TTAGGAAGGA TTAAAGATAA TCTCTTTTCT ATGCTTTTTT AAAAACTTAG AGATCTTGAT 
AGAATTTAAG TTCTTCTCTT TTATGACACT GCTCTTTATC CATAAAATTT AGAAGAAATT 
AAATTATTCA TGAATTGGCA ACTTGGAAAA GTTAAAAAGA TAGGATTTGT CTAAAACTTT 
TTGAATCAGC TAGACTTGAA CTTCTTATTT GATACATCTC TCACTAAATA CCCTAGAAAT 
AACTAATAAT TAAACGATAT TTATTTTTTA AAATACTTTA ACTCAAATTT AGTTAAGAGT 
TACTCATTAA TCATGATAGA TCAAAATTAT ATCTCATAAA ACCTTTAATC ACTCGTTTAA 
CAAAATTTAA AACCTATTTC ATAAAAGGGA ATATACTACA ACCCAAATTT AGGGTATGAT 
TTTAAATTCA ATTAAGCAAA GTTAGAAACA TTGGATTTAA AAACTTAATG GCTAAGCATA 
AAACATTCTA TTGAAAATGA TCAAATTGAG GACTTATAGA TTGAATATTA TAATGATTTT 
TTAATCGATA ATAAGAATGA GGTAGGACAT AATTTTGATT TAACTATTTA CTCAAGAAAA 
TCTTTAAAAG ACATAGATAT TAACCTAATA TATTTCAATA TTAATCAAGT TGAAAGAGTA 
CTCTCTTTAA GAAATAAAAT TGGGATGTAT TGTCTTAAGA TTTTATCTAA CATAGATAAT 
TATTTAAAAA ATTTAAAGCT TAGAGATTTG TTGGTATAAA AGTATGTTGG CTCAACTCTT 
ACTATTGTAA ATTTAGATAT TTTATTTGTT CCTACTCTCG AAAATATTTC TCTGTTTTTA 
TAGTCCGAAA TAGCTCAGCT AAGCTAAGGA TTGAATAAAT GTGCTCCGTA TTTAAAAAGA 
TTTAAAATTT CTGGAGATGT AGGATAACTT ATACCTATGA ATAATTTATT AAAAGGAGTA 
GATTGGAGTA AATTCAAGTA ATTATAAAAA ATCATATTTT CAAGATGTAA TTTTGATGAG 
GAAAGCTTAT TTGAATTATT TAATTCACTA AATGATACAA GCTTAGAAAA AATTAAATTA 
GTAAGTATCA AATGTAGTGA GAAATTCTTA TATGCAATCG ATTGGAAAAG ATTCAAACAA 
ATTAATTTCA TAGAATTAAC ATCAGTAATT TTTAACATAA AAAATGATAA TAAAAGCTTT 
TATGTAACTG ATATTTGCGA ACCTATAGCT CATTAACTCA AAACACTTAA TTTTAACCGT 
ATAAATATAC CTTAGCATTA CTTGGGTGCA TTTAATCCAT CAAAATATAC AAATCTTACA 
AGTTTTAAAG CCAGTAAATT TTTCTAAAAC TATCAAAATA TGCTATAAAT GGTAAATATC 
AAATAGCTTT AATAGAAAAA TGATAATGGT GAAAAATAAA AAAAAGATGA AAATGCTAAT 
CAAAAAAAAA ATATAGAATT GAGTAATTAA AACAATACTT AAAAAAATGA TACAAATAAT 
CAAGAAGGCA ATGAAGAAGA AGAAGAGGTT TTAGACCATG AATAAAAAAC AGCAAAGATT 
TATCAAAAAA TATAAAATCA GATAATAAAA GATGCTATTA AAGAAGATGA TAAAAAATAA 
TTGTTTGTTT AAAAGTAAGA CGAACTTACA GATTAAATTG TTTATAAATC ATTTGATAGA 
ATTAGCATAA AGCTTAACTC TGATTATATC TTTAGTCAAT ATAATTGTGA AAATGTAGAT 
TTAAAATACT TAGACATGTG TGTAAAATCA ATCAATTTAA CCACCTTATC TCCTTCATAG 
CTTACTAATT ATGATTAAAA AATAATATTT AGTAAACTAT AAAATGTTGA AGAAATATTT 
TGCTGGTAAA ATTTCAATGA TAAAGAAGCA AAAAAACATA ATTCATAACT GGAAAGTATT 
ATTAATTAAT TATCCAACTA ATTAAAAGTG TTAGATTTAA GAAGGAGTAG TATAAGTTTA 
GTAAAAATTT TTGACTCTTA AAATCAAAAA TAATTTTCGC AATTAAAAAT TTTAAAAGTA 
ACAAGATCGT TCTTCTTTTC GTTCAAACGA ATTTAAGCTT TCATTGATAG AATATCTTCA 
ACTATTGAAT GTATAGAAAT ATTAAATATT ACAGAAAGCT AATAAAATTA AACTAAGAAA 
GATAATTAAA TCATTGATTT TTCTAAATTA GTCAATCTTC GAACTTTCAT TATTCATGAT 
TCTATTAACA ATTAGGTTTA AAAACCTTTA TAAACCTTAC CAAAATAAAT TTAAAACTTA 
AAACTGTTTT CTCTAAAATA AAATTTAGAT TTTTTGTAAA TAAATGAATT TAAATAGCTT 
ACAAATTTAA CAATTTCTAA CAATCAAAAT CTTATATAAA AAAATAAATC CTTTTTTGAT 
TAAAAAAATA AATCTATTAA GTTTTTGTGT CTAAAAAATA ATTCTCTAAG AATGTAATAG 
TTAGAAAATC TTAAAATTAA TAATTTAGAA AATCTAGAGA CTCTTATTTT AAATCATAAT 
CCACTTTACA AAGATAATTT AGATAATTAT GCAAGCTTTT TTAACTCTGA AGAAAGCTTC 
GATTCATTAA AACAAGAGGA AATTTTTTAA GGTGAAAATT AGTATGCCAG TGATTAAGAA 
GAGGAAGAAA GTAATACTGA TGAAGATGAA GAAGAAGACG ATGATGATGA TCATGATGAA 
TAAGAAGAAG ACGATGATGA TGATGAAGAA GAAGAAGAAG AGGATAGTGA AAATGAAGAG 
AATGAAAGCT AATATGAAGA TTCGGAAATT AATAGTGATG AAGAAAATAG TGAGGAAGAT 
GAAGAAAGAA GTGGAGAGGA TAGTTCATAA GATTTAAGCA TTAAAAAAAA AAAGAAAGTA 
TAGAAAAAAT AAAATAAATA AAAAAAATTA AAAAAAATGA AAATCAGTAA ATAAAATAAA 
AATGTTAATC CAAAAAAGAG TGGTTTTAAA TTTTTTTAAA TACTAGAATC ATTTAAAAGT 
AGTTTGATAA ATTTATGCAT GAGAAATTGT AATATAACTG AAGAAATATT TGATAAAGTA 
GATTTTACAA ATTTTAAGCA ACTCTCAAAG CTTGATATTT CAAGAAACAA TAACTTTTTC 
TTGTAAAATA AGCAAATATT TAAAAATATG CTAAACTAAA TATCTTGGAA CTTGTAAGAA 
CTAAATGTTG GCCAAACAAA TATTTGTCTT GAAAATAATC TATCTAATTT CGATTTTACA 
AAATTATAGA AGTTATCTAT TTTTAAGGCT AAATGGGCTT TAAAAATATT TAAATCATCT 
TAAAGCAACT AGACATTTTA ATCAAAAGAA GCTTTATTAT TTATGAATGC TATAATATCT 
TAATTATCTC AAATTTAGTT AACTGAATTA GTTATTGGAG CAAACTGGCA GTAGGCTAGT 
AAGCTAAAAT TTTTAGATAT CAGTAATATG AATTTAGAAA AATTCTTAGA AGTTTAGAGA 
AGAGATTAAG TAATCGAACT ATAAAAGTTA ATTTAAAATG TAGGGAAAAG ATTAGAAGTT 
TTAAATATGA ATTAATTAAT GATACAAGCT TAAGAATTAG AGATGATAAA TTGGCAAAAC 
CTTATTAATT TGAAATTATT AAATATATCA AATATCGATT GTTTTGAATA GAATTTTCAT 
ATTATTGGAA AAATTACTGA AAAAATGATT AAATTAGAGC AGATGATTTT AAAAGACGAA 
GGTATTGAAG AATAAATACT TACTTTACCA TGGAAAAATT TTTCTAATAT AAGAAAGGTT 
ATTTTGGAAA AGGATTAATA AACAAATATT TATTATTATT CATTTTAGAA TAACATTTTT 
AAGAGTTACT TGAAAAACGA TTATAGTTTT GCCATGATGC CTCTTTAATT ATTTAATGCT 
AGAATTTTGA ATCCTTCTCA CGCTTATTAC TAATAATATT TAAAATTATA GAACGAAAAA 
GAAGACTTTT CAAATAAAAA TCACTTGAAT AGATTAGAAT AAAATTAATA AAAAACTTTG 
ATTTTAGATA GAATCAAATA CAATTTTCCA TTTTAACCTC TTTCAGAAAT AGACGAGAAA 
TATGCTTCGC AAATGTCTTA TTCCACTTAT GGGATGGTTA TGTGGAATTT GGTTCGATTT 
GACATTCTTT TGAAAGCTGA TTTAAATTAG CTTTAAAAAA TTCTTTCTCA ATAAGCATTT 
AATTAAAAAA TAATATATTT TAATAATATA AATTGCTATT TGTTTTTTAT TTTCTCTAAA 
ATAAATGATG AAAGCTTTTT AGGTTTGATT TTAAACTCTT ACCTACTTGA ATCAAATCAA 
ATAGAAACAG TATTTATTTA ACAATTGAAT GACGATAAAT TTTTTTATTC AGGAAGCATA 
AATATTTCTG TTATCTATAA GCTAATGGAT ATTTTTTTCA ATAGACCAAC AAAAAGGATA 
ATTCTTAGCT ATTCCCTAAA TTTAAACAGA AATTAGATTA TGAATGAATT AACTTCCTCC 
TCTTAAAAAG AAAGTCTTTA CGAAGAAGAA TAATTCAGCT CTTCTTTTTA AGAAAATTTA 
GACCAAAACT CATAAAAATT TGATAAAATT GTAGAGCTTA ACAGAAATCT GAATTTTATA 
AATGAATATA CTATGCTAGA CTTTTTGAAT TTTGCTCCAT CACAAGAATT AAATCTATCA 
AATTACAGTA TTTAATTTTT TAAATATCTC TATCTCATAT AAATGTATTC TCCTTACAGC 
TATAAGACCA CCATAAACTA TTCGATTGAT TCATTGTCAA ATAATAGTAT TTCGGCCAGG 
TTGTTAAAAA TTTTGTACTA AAGAGGAAAA AATGGAAAAC TCTACAAATT TTTAAACTTA 
TTTTATTAGG ACACAACTTT TTACAAATTC GATGAATCTG TTATTTTACT AAATACAAAG 
CTTTCTTTAT CGAAAGAAAA TATATTTTTA AAATAATTAA TGCTCTTTAT GATTCCTATT 
TATTATTTTA TTTGCATATT CGGTCCTTTA TATTTCACTT ATAGGTAAGA TTTAATAGAT 
TCATGTGGTA AAGGTCTGTC ATGGCACTCT TACTATGTTT ATCTTGCCTT TGCAATAATC 
AGTTTTTTGA TGGAATATTC TTTGTATTCT AAAATATTAA GCACAACTTC TCATTTAATC 
CCTGCGGCTT CAATAACTCC TTCTACTTCT TTAATAGGAA TTTTATTAAG TGAAATTAAA 
AAAGTGATAA ATCAGAATAG AAGCTATCTC TATATTTTTT CTCTAGCAAG CTCTTAGATT 
ACAAGATACA ATTACTTCAC AGACATAGGT TTCATTGTTA GTTGCTATTA ATGCCAAAGA 
CCTAAACTAA TGATTGCATC AATAGTTTTT GTAGGATTAC ATACTCTGAT AAATTTAATA 
TATTATATCC TACTGCTTTT CTAAAAGTCA AAAAAATTTT TAATTCCAAC TTCTGAAATT 
GATAAATTTT ATCAAATTTC AACATTACTT GAATTTCACG CTGTCTCAGA TACACTAGAT 
TTAATATCAC CTTCTAATGT TGTTGTATTT CCTGTTTTTT TAAGAAAAAT CAGTTTATTC 
AGATTGAGAA CTATAGCAGG GATAGCTTTT AACGGAAGAA TTTATAATGC AATACAAAGA 
TTTTTACTCG AAGATTTTCC ATAGCTTATA ATTTAGATAA TATTTCTTAT TTTAGAAAAT 
AAGGCAAATA TTAACATTAT ACTTTCATTT GTAGCTACCA TAGTTACTTT GCTTATATCA 
ATGTATAAGT TCCTTTCAAT TAGACCATCT ACAATAAATT AGCTTGATTT TGATTTTTTG 
AACTAAACAA AAAAAGAAAA TTCATGGAAA TTTGAAAAAA AGATACACCA ACTCTCAAAA 
AAAACAAATT AAAATATAAT CGATAATATC AAAAAACTTA ATAGAAATAC AGAATATTCA 
TAAATACTAA ATGATTTCTA TAAATTTAAT TAATTAGATT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_001022656.2
CDS	1..7602
Translation	MNSNSNKPDGQILKKATLQRLNSRINEFIKSKLLMKNDYKQKKFHNIPSRSILICQDEDNLRSFFVNSANIDNKIEEKEKYVGEINFISVDDKNNEEYEEDISDVESNKDDNDDFIESQLISDKAEKEYLEECLGIKLKQIESVALNEKIYDNNKEKETDLTNNEKSLLRNKLSGSKTNIIKHNDEQNIQLPQNNLSTK*SGQMKNSTINKKLIEFNIKIIMKNKDKNLILEYGDFLDLIEEVTFENIRTINVFKILKVFRIKILRVVYSIFDINSCRYIFDCKNYLENIFFLRSSPAVFSSRIFKKADIENITRESAMFIMFQDYLKIYIEIDIVLDLLELNTFSVRIINYNPLILVDYLLGEYRLILNTNEEDQKSKNTLDFEEDYLSRLEEGTADFYNYLFLDIKNEESLNDEEKNLKIKFYKSVSSIEDIFRKKKFDSFVIEKRFSSKKNPKKVKTRIELIINKFHFEDIPSFIYLTTIIDYSSIITSIKFMRLGRIKDNLFSMLFKLRDLDRIVLLFYDTALYPNLEEIKLFMNWQLGKVKKIGFVNFLNQLDLNFLFDTSLTKYPRNNLNDIYFLKYFNSNLVKSYSLIMIDQNYISNLSLVQNLKPISKGIYYNPNLGYDFKFNAKLETLDLKTWLSIKHSIENDQIEDLIEYYNDFLIDNKNEVGHNFDLTIYSRKSLKDIDINLIYFNINQVERVLSLRNKIGMYCLKILSNIDNYLKNLKLRDLLVKYVGSTLTIVNLDILFVPTLENISLFLSEIAQLSGLNKCAPYLKRFKISGDVGLIPMNNLLKGVDWSKFKLKIIFSRCNFDEESLFELFNSLNDTSLEKIKLVSIKCSEKFLYAIDWKRFKQINFIELTSVIFNIKNDNKSFYVTDICEPIAHLKTLNFNRINIPHYLGAFNPSKYTNLTSFKASKFFNYQNMLMVNIKLKNDNGEKKKDENANQKKNIELSNNNTKNDTNNQEGNEEEEEVLDHEKTAKIYQKINQIIKDAIKEDDKKLFVKDELTDIVYKSFDRISIKLNSDYIFSQYNCENVDLKYLDMCVKSINLTTLSPSLTNYDKIIFSKLNVEEIFCWNFNDKEAKKHNSLESIINLSNLKVLDLRRSSISLVKIFDSNQKFSQLKILKVTRSFFFSFKRIAFIDRISSTIECIEILNITES*NTKKDNIIDFSKLVNLRTFIIHDSINNVKPLTLPKINLKLFSLKNLDFLINEFKLTNLTISNNQNLIKNKSFFDKNKSIKFLCLKNNSLRMLENLKINNLENLETLILNHNPLYKDNLDNYASFFNSEESFDSLKQEEIFGENYASDEEEESNTDEDEEEDDDDDHDEEEDDDDDEEEEEEDSENEENESYEDSEINSDEENSEEDEERSGEDSSDLSIKKKKKVKKNKKKLKKMKISKNKNVNPKKSGFKFFILESFKSSLINLCMRNCNITEEIFDKVDFTNFKQLSKLDISRNNNFFLNKQIFKNMLNISWNLELNVGQTNICLENNLSNFDFTKLKLSIFKAKWALKIFKSSSNTFSKEALLFMNAIISLSQILTELVIGANWQASKLKFLDISNMNLEKFLEVRRDVIELKLINVGKRLEVLNMNLMIQAELEMINWQNLINLKLLNISNIDCFENFHIIGKITEKMIKLEQMILKDEGIEEILTLPWKNFSNIRKVILEKD*TNIYYYSFNNIFKSYLKNDYSFAMMPLLFNARILNPSHAYYYLKLNEKEDFSNKNHLNRLENKTLILDRIKYNFPFPLSEIDEKYASQMSYSTYGMVMWNLVRFDILLKADLNLKILSQAFNKIIYFNNINCYLFFIFSKINDESFLGLILNSYLLESNQIETVFIQLNDDKFFYSGSINISVIYKLMDIFFNRPTKRIILSYSLNLNRNIMNELTSSSKESLYEEEFSSSFENLDQNSKFDKIVELNRNLNFINEYTMLDFLNFAPSQELNLSNYSIFFKYLYLIMYSPYSYKTTINYSIDSLSNNSISARLLKILYRGKNGKLYKFLNLFYDTTFYKFDESVILLNTKLSLSKENIFLKLMLFMIPIYYFICIFGPLYFTYRDLIDSCGKGLSWHSYYVYLAFAIISFLMEYSLYSKILSTTSHLIPAASITPSTSLIGILLSEIKKVINQNRSYLYIFSLASSITRYNYFTDIGFIVSCYCQRPKLMIASIVFVGLHTLINLIYYILLLFKSKKFLIPTSEIDKFYQISTLLEFHAVSDTLDLISPSNVVVFPVFLRKISLFRLRTIAGIAFNGRIYNAIQRFLLEDFPLIIIIFLILENKANINIILSFVATIVTLLISMYKFLSIRPSTINLDFDFLNTKKENSWKFEKKIHQLSKKTNNIIDNIKKLNRNTEYSILNDFYKFNLD

Target ORF information:

RefSeq Version	XM_001022656.2
Organism	Tetrahymena thermophila SB210
Definition	Tetrahymena thermophila SB210 transmembrane protein, putative partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_001022656.2

ORF Insert Sequence:

ATGAATTCTA ACTCAAATTA AAAACCTGAT GGTCAAATAC TAAAAAAGTA AGCAACTCTA 
CAAAGATTAA ATTCTCGAAT AAATGAATAA TTCATTAAAA GCAAGCTCCT TTAAATGAAA 
AACGATTATA AACAAAAGAA ATTTCATAAT TAGATACCCT CAAGATCAAT TTTAATATGC 
CAAGACGAAT AAGATAATCT ACGCTCATTT TTTGTATAAA ATTCAGCTAA TATAGATAAT 
AAAATTGAAG AAAAAGAAAA ATATGTAGGG GAAATTTAGA ATTTTATTAG TGTAGATGAT 
AAAAATAATG AAGAATACGA AGAAGATATT TCTGACGTTG AAAGCAACAA AGATGATAAC 
TAAGATGATT TTATTTAAGA AAGCCAATTA ATATCTGATA AAGCTGAAAA GGAATACTTA 
GAAGAATGTT AGCTTGGTTA AATTAAATTA AAGCAAATAG AATCTGTAGC GTTGAATGAA 
AAAATATATG ATAATAACAA AGAAAAAGAA ACTGATTAAC TAACAAATAA CGAAAAATCT 
TTATAATTGA GAAATAAGTT AAGTGGTAGT AAGACTAATA TTATATAAAA ATAGTAACAT 
AATGATGAGC AAAATATTCA ATTACCTCAG TAAAATAATT TATCTTAAAC AAAATAATAA 
TAATCAGGTC AAATGAAAAA CTCTACAATC AATTAAAAAA AATTAATTGA ATTTAATATA 
AAAATTATCA TGAAAAATTA AAAATAGGAT AAAAATTTAA TCTTAGAATA TGGTGATTTC 
CTTGATTTGA TTGAAGAAGT AACATTCGAG AATATAAGGA CAATAAATGT ATTCAAGATA 
TTAAAAGTAT TTTAAAGAAT AAAGATTTTG AGAGTTGTTT ATTCAATATT CGATATCAAT 
AGCTGCAGAT AGTATATATT TGATTGTAAA AATTATTTAG AAAATATATT CTTTTTGAGA 
TCTAGTCCAG CTGTATTTTC TAGTAGAATT TTCAAAAAAG CAGATTAGAT AGAAAATATA 
ACCAGAGAAT CAGCGATGTT TATAATGTTT TAACAAGATT ATTTAAAAAT CTAATATATT 
GAATAGATTG ATTAAATAGT CTTAGATTTG TTAGAGTTAA ATACTTTTTA ATCAGTAAGA 
ATTATCTAAA ATTACAATCC TCTCATATTA GTAGATTATT AGTAATTACT TGGTGAATAT 
AGATAACTTA TTTTATAAAA TACTAATGAG GAAGACCAAA AGTCTAAAAA TACTTTATAA 
GATTTTGAAG AGGATTAATA TTTATCCAGA TTAGAAGAAG GAACAGCTGA TTTTTATAAT 
TACCTGTTTC TAGATATTAA AAACGAAGAA TCTTTAAATG ACGAAGAAAA AAATTAACTT 
AAATAAATAA AATTTTATAA AAGTGTCTCT AGTATAGAGT AAGACATTTT TAGGAAAAAG 
AAATTCGATA GCTTTGTAAT AGAGAAGCGT TTTTCGAGTA AGAAGAATCC AAAAAAAGTA 
AAAACAAGAA TAGAACTTAT AATAAATAAA TTTCATTTTG AAGACATACC ATCCTTTATT 
TACTTGACTA CTATAATAGA CTAGTATTCT AGTATAATTA CGTCTATTAA GTTTATGAGA 
TTAGGAAGGA TTAAAGATAA TCTCTTTTCT ATGCTTTTTT AAAAACTTAG AGATCTTGAT 
AGAATTTAAG TTCTTCTCTT TTATGACACT GCTCTTTATC CATAAAATTT AGAAGAAATT 
AAATTATTCA TGAATTGGCA ACTTGGAAAA GTTAAAAAGA TAGGATTTGT CTAAAACTTT 
TTGAATCAGC TAGACTTGAA CTTCTTATTT GATACATCTC TCACTAAATA CCCTAGAAAT 
AACTAATAAT TAAACGATAT TTATTTTTTA AAATACTTTA ACTCAAATTT AGTTAAGAGT 
TACTCATTAA TCATGATAGA TCAAAATTAT ATCTCATAAA ACCTTTAATC ACTCGTTTAA 
CAAAATTTAA AACCTATTTC ATAAAAGGGA ATATACTACA ACCCAAATTT AGGGTATGAT 
TTTAAATTCA ATTAAGCAAA GTTAGAAACA TTGGATTTAA AAACTTAATG GCTAAGCATA 
AAACATTCTA TTGAAAATGA TCAAATTGAG GACTTATAGA TTGAATATTA TAATGATTTT 
TTAATCGATA ATAAGAATGA GGTAGGACAT AATTTTGATT TAACTATTTA CTCAAGAAAA 
TCTTTAAAAG ACATAGATAT TAACCTAATA TATTTCAATA TTAATCAAGT TGAAAGAGTA 
CTCTCTTTAA GAAATAAAAT TGGGATGTAT TGTCTTAAGA TTTTATCTAA CATAGATAAT 
TATTTAAAAA ATTTAAAGCT TAGAGATTTG TTGGTATAAA AGTATGTTGG CTCAACTCTT 
ACTATTGTAA ATTTAGATAT TTTATTTGTT CCTACTCTCG AAAATATTTC TCTGTTTTTA 
TAGTCCGAAA TAGCTCAGCT AAGCTAAGGA TTGAATAAAT GTGCTCCGTA TTTAAAAAGA 
TTTAAAATTT CTGGAGATGT AGGATAACTT ATACCTATGA ATAATTTATT AAAAGGAGTA 
GATTGGAGTA AATTCAAGTA ATTATAAAAA ATCATATTTT CAAGATGTAA TTTTGATGAG 
GAAAGCTTAT TTGAATTATT TAATTCACTA AATGATACAA GCTTAGAAAA AATTAAATTA 
GTAAGTATCA AATGTAGTGA GAAATTCTTA TATGCAATCG ATTGGAAAAG ATTCAAACAA 
ATTAATTTCA TAGAATTAAC ATCAGTAATT TTTAACATAA AAAATGATAA TAAAAGCTTT 
TATGTAACTG ATATTTGCGA ACCTATAGCT CATTAACTCA AAACACTTAA TTTTAACCGT 
ATAAATATAC CTTAGCATTA CTTGGGTGCA TTTAATCCAT CAAAATATAC AAATCTTACA 
AGTTTTAAAG CCAGTAAATT TTTCTAAAAC TATCAAAATA TGCTATAAAT GGTAAATATC 
AAATAGCTTT AATAGAAAAA TGATAATGGT GAAAAATAAA AAAAAGATGA AAATGCTAAT 
CAAAAAAAAA ATATAGAATT GAGTAATTAA AACAATACTT AAAAAAATGA TACAAATAAT 
CAAGAAGGCA ATGAAGAAGA AGAAGAGGTT TTAGACCATG AATAAAAAAC AGCAAAGATT 
TATCAAAAAA TATAAAATCA GATAATAAAA GATGCTATTA AAGAAGATGA TAAAAAATAA 
TTGTTTGTTT AAAAGTAAGA CGAACTTACA GATTAAATTG TTTATAAATC ATTTGATAGA 
ATTAGCATAA AGCTTAACTC TGATTATATC TTTAGTCAAT ATAATTGTGA AAATGTAGAT 
TTAAAATACT TAGACATGTG TGTAAAATCA ATCAATTTAA CCACCTTATC TCCTTCATAG 
CTTACTAATT ATGATTAAAA AATAATATTT AGTAAACTAT AAAATGTTGA AGAAATATTT 
TGCTGGTAAA ATTTCAATGA TAAAGAAGCA AAAAAACATA ATTCATAACT GGAAAGTATT 
ATTAATTAAT TATCCAACTA ATTAAAAGTG TTAGATTTAA GAAGGAGTAG TATAAGTTTA 
GTAAAAATTT TTGACTCTTA AAATCAAAAA TAATTTTCGC AATTAAAAAT TTTAAAAGTA 
ACAAGATCGT TCTTCTTTTC GTTCAAACGA ATTTAAGCTT TCATTGATAG AATATCTTCA 
ACTATTGAAT GTATAGAAAT ATTAAATATT ACAGAAAGCT AATAAAATTA AACTAAGAAA 
GATAATTAAA TCATTGATTT TTCTAAATTA GTCAATCTTC GAACTTTCAT TATTCATGAT 
TCTATTAACA ATTAGGTTTA AAAACCTTTA TAAACCTTAC CAAAATAAAT TTAAAACTTA 
AAACTGTTTT CTCTAAAATA AAATTTAGAT TTTTTGTAAA TAAATGAATT TAAATAGCTT 
ACAAATTTAA CAATTTCTAA CAATCAAAAT CTTATATAAA AAAATAAATC CTTTTTTGAT 
TAAAAAAATA AATCTATTAA GTTTTTGTGT CTAAAAAATA ATTCTCTAAG AATGTAATAG 
TTAGAAAATC TTAAAATTAA TAATTTAGAA AATCTAGAGA CTCTTATTTT AAATCATAAT 
CCACTTTACA AAGATAATTT AGATAATTAT GCAAGCTTTT TTAACTCTGA AGAAAGCTTC 
GATTCATTAA AACAAGAGGA AATTTTTTAA GGTGAAAATT AGTATGCCAG TGATTAAGAA 
GAGGAAGAAA GTAATACTGA TGAAGATGAA GAAGAAGACG ATGATGATGA TCATGATGAA 
TAAGAAGAAG ACGATGATGA TGATGAAGAA GAAGAAGAAG AGGATAGTGA AAATGAAGAG 
AATGAAAGCT AATATGAAGA TTCGGAAATT AATAGTGATG AAGAAAATAG TGAGGAAGAT 
GAAGAAAGAA GTGGAGAGGA TAGTTCATAA GATTTAAGCA TTAAAAAAAA AAAGAAAGTA 
TAGAAAAAAT AAAATAAATA AAAAAAATTA AAAAAAATGA AAATCAGTAA ATAAAATAAA 
AATGTTAATC CAAAAAAGAG TGGTTTTAAA TTTTTTTAAA TACTAGAATC ATTTAAAAGT 
AGTTTGATAA ATTTATGCAT GAGAAATTGT AATATAACTG AAGAAATATT TGATAAAGTA 
GATTTTACAA ATTTTAAGCA ACTCTCAAAG CTTGATATTT CAAGAAACAA TAACTTTTTC 
TTGTAAAATA AGCAAATATT TAAAAATATG CTAAACTAAA TATCTTGGAA CTTGTAAGAA 
CTAAATGTTG GCCAAACAAA TATTTGTCTT GAAAATAATC TATCTAATTT CGATTTTACA 
AAATTATAGA AGTTATCTAT TTTTAAGGCT AAATGGGCTT TAAAAATATT TAAATCATCT 
TAAAGCAACT AGACATTTTA ATCAAAAGAA GCTTTATTAT TTATGAATGC TATAATATCT 
TAATTATCTC AAATTTAGTT AACTGAATTA GTTATTGGAG CAAACTGGCA GTAGGCTAGT 
AAGCTAAAAT TTTTAGATAT CAGTAATATG AATTTAGAAA AATTCTTAGA AGTTTAGAGA 
AGAGATTAAG TAATCGAACT ATAAAAGTTA ATTTAAAATG TAGGGAAAAG ATTAGAAGTT 
TTAAATATGA ATTAATTAAT GATACAAGCT TAAGAATTAG AGATGATAAA TTGGCAAAAC 
CTTATTAATT TGAAATTATT AAATATATCA AATATCGATT GTTTTGAATA GAATTTTCAT 
ATTATTGGAA AAATTACTGA AAAAATGATT AAATTAGAGC AGATGATTTT AAAAGACGAA 
GGTATTGAAG AATAAATACT TACTTTACCA TGGAAAAATT TTTCTAATAT AAGAAAGGTT 
ATTTTGGAAA AGGATTAATA AACAAATATT TATTATTATT CATTTTAGAA TAACATTTTT 
AAGAGTTACT TGAAAAACGA TTATAGTTTT GCCATGATGC CTCTTTAATT ATTTAATGCT 
AGAATTTTGA ATCCTTCTCA CGCTTATTAC TAATAATATT TAAAATTATA GAACGAAAAA 
GAAGACTTTT CAAATAAAAA TCACTTGAAT AGATTAGAAT AAAATTAATA AAAAACTTTG 
ATTTTAGATA GAATCAAATA CAATTTTCCA TTTTAACCTC TTTCAGAAAT AGACGAGAAA 
TATGCTTCGC AAATGTCTTA TTCCACTTAT GGGATGGTTA TGTGGAATTT GGTTCGATTT 
GACATTCTTT TGAAAGCTGA TTTAAATTAG CTTTAAAAAA TTCTTTCTCA ATAAGCATTT 
AATTAAAAAA TAATATATTT TAATAATATA AATTGCTATT TGTTTTTTAT TTTCTCTAAA 
ATAAATGATG AAAGCTTTTT AGGTTTGATT TTAAACTCTT ACCTACTTGA ATCAAATCAA 
ATAGAAACAG TATTTATTTA ACAATTGAAT GACGATAAAT TTTTTTATTC AGGAAGCATA 
AATATTTCTG TTATCTATAA GCTAATGGAT ATTTTTTTCA ATAGACCAAC AAAAAGGATA 
ATTCTTAGCT ATTCCCTAAA TTTAAACAGA AATTAGATTA TGAATGAATT AACTTCCTCC 
TCTTAAAAAG AAAGTCTTTA CGAAGAAGAA TAATTCAGCT CTTCTTTTTA AGAAAATTTA 
GACCAAAACT CATAAAAATT TGATAAAATT GTAGAGCTTA ACAGAAATCT GAATTTTATA 
AATGAATATA CTATGCTAGA CTTTTTGAAT TTTGCTCCAT CACAAGAATT AAATCTATCA 
AATTACAGTA TTTAATTTTT TAAATATCTC TATCTCATAT AAATGTATTC TCCTTACAGC 
TATAAGACCA CCATAAACTA TTCGATTGAT TCATTGTCAA ATAATAGTAT TTCGGCCAGG 
TTGTTAAAAA TTTTGTACTA AAGAGGAAAA AATGGAAAAC TCTACAAATT TTTAAACTTA 
TTTTATTAGG ACACAACTTT TTACAAATTC GATGAATCTG TTATTTTACT AAATACAAAG 
CTTTCTTTAT CGAAAGAAAA TATATTTTTA AAATAATTAA TGCTCTTTAT GATTCCTATT 
TATTATTTTA TTTGCATATT CGGTCCTTTA TATTTCACTT ATAGGTAAGA TTTAATAGAT 
TCATGTGGTA AAGGTCTGTC ATGGCACTCT TACTATGTTT ATCTTGCCTT TGCAATAATC 
AGTTTTTTGA TGGAATATTC TTTGTATTCT AAAATATTAA GCACAACTTC TCATTTAATC 
CCTGCGGCTT CAATAACTCC TTCTACTTCT TTAATAGGAA TTTTATTAAG TGAAATTAAA 
AAAGTGATAA ATCAGAATAG AAGCTATCTC TATATTTTTT CTCTAGCAAG CTCTTAGATT 
ACAAGATACA ATTACTTCAC AGACATAGGT TTCATTGTTA GTTGCTATTA ATGCCAAAGA 
CCTAAACTAA TGATTGCATC AATAGTTTTT GTAGGATTAC ATACTCTGAT AAATTTAATA 
TATTATATCC TACTGCTTTT CTAAAAGTCA AAAAAATTTT TAATTCCAAC TTCTGAAATT 
GATAAATTTT ATCAAATTTC AACATTACTT GAATTTCACG CTGTCTCAGA TACACTAGAT 
TTAATATCAC CTTCTAATGT TGTTGTATTT CCTGTTTTTT TAAGAAAAAT CAGTTTATTC 
AGATTGAGAA CTATAGCAGG GATAGCTTTT AACGGAAGAA TTTATAATGC AATACAAAGA 
TTTTTACTCG AAGATTTTCC ATAGCTTATA ATTTAGATAA TATTTCTTAT TTTAGAAAAT 
AAGGCAAATA TTAACATTAT ACTTTCATTT GTAGCTACCA TAGTTACTTT GCTTATATCA 
ATGTATAAGT TCCTTTCAAT TAGACCATCT ACAATAAATT AGCTTGATTT TGATTTTTTG 
AACTAAACAA AAAAAGAAAA TTCATGGAAA TTTGAAAAAA AGATACACCA ACTCTCAAAA 
AAAACAAATT AAAATATAAT CGATAATATC AAAAAACTTA ATAGAAATAC AGAATATTCA 
TAAATACTAA ATGATTTCTA TAAATTTAAT TAATTAGATT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

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TTHERM_00727710 cDNA ORF clone, Tetrahymena thermophila SB210

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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